LOCUS       DAOSCR010000001        19161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_2005_length_19161_cov_3.758191, whole genome shotgun sequence.
ACCESSION   DAOSCR010000001 DAOSCR010000000
VERSION     DAOSCR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2005_length_19161_cov_3.758191"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..745)
                     /locus_tag="PLT69_00005"
     CDS             complement(185..745)
                     /locus_tag="PLT69_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ12862.1"
                     /translation="MERFRRKDTRGFTLVELALVLVIIGLLITGVLKGEALIQNAKIK
                     KLVNQKDSLTAAYYAFYDRYNVYPGDENQASIPPNDTHNGNQDGQITGNEYWYLFEDL
                     VLAQIINGNYNGTANSCPQNPYGSWAQIQWNAALQSNAVVFNAIPYDAAEQIDMKYDD
                     GIYNTGAIRANSAYTTQTNKILYWRM"
     gene            complement(1140..2222)
                     /locus_tag="PLT69_00010"
     CDS             complement(1140..2222)
                     /locus_tag="PLT69_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12863.1"
                     /translation="MPTPEGLAPAGVTASLPPVVHPGGVIHLLNAGGRLDVSGVGADV
                     WSRARRSVADPGECFACQDIVLRGAARSVALSGRMLACTTSRDAAVLIDLAGGGIEWL
                     FEGAGGVVSIRKDRVLAGPNASGDLSSVAWGSGRIWSRKASSWDASASTVYVADGASL
                     TAVGIDDGEPVCSIDAGHEITVVRYDPVTGEVAAGTSDGLVLGFDADLSWSSVLATGD
                     EEAVTDVRSFTLSGRRHVLAVTPDRVHLFSGAALTWSASIAPGRITGVTVMDAKVYLV
                     VWRVQDGIRGADEGVSALVVLDASTGGILEDTTLCTGVAFGPSLDADAGLLRLISFSG
                     QPLEIDVSSLPLLTPRFMGMKIRGVP"
     gene            complement(2232..3284)
                     /locus_tag="PLT69_00015"
     CDS             complement(2232..3284)
                     /locus_tag="PLT69_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12864.1"
                     /translation="MRFQNTCTSAFPSGERGFILALCLVFLTATIALTLAAASLALVH
                     LKSAGAFADILGRTLLAGEGDDAKRTSTFLGAPEGWDHIHFSSVLVETRTEGACAYSM
                     RVMQSQPCSPILPMDLVPARSPGILVLLDDSQLMRISSGFDYEPQALYLKNAAGQIVR
                     VSGCSEIASSLATTAGTFFRGDFGNSHERAPTGSSRTGAMSCWTFALSNVYHLLAGLD
                     HACFGLESTSGGLRAPFSPSPGPITRVLASACPTAPEAPLAEALFRAIDRFNDPRLEP
                     RHIVVATAGIPLSDGDLPGWLRDYDRDGDPMDTAVEGEGSHCLDDVAAYARSRGVRVH
                     VIGPDTPLLRAAASKG"
     gene            complement(3241..>3998)
                     /locus_tag="PLT69_00020"
     CDS             complement(3241..>3998)
                     /locus_tag="PLT69_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ12865.1"
                     /translation="EGARPLEHADRVAEGPGGLFADPPEKGMSLVELMVALACASLVT
                     IGLYHLYLVSSTFYKAARDTWSCMQSLRAASLELHTDLAQAACLMPRSLAFRVTSNGL
                     FIAGIPVTSQHPGIQVHPVKAPPYYAVIAETGGAALTLDTVDIDSDGRPDFWAFLGVI
                     TDTSAGVVSGSYRRGSLRVPIGHPADVRAGERIVPAVHYELKPDGLYRNSQLLAEAIT
                     RFEPRALGGDLSIFLEAASHEVSKHVHFRFPVR"
     assembly_gap    3999..4008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4355..6010
                     /locus_tag="PLT69_00025"
     CDS             4355..6010
                     /locus_tag="PLT69_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ12866.1"
                     /translation="MSHKPSYGNATIVVIVGMAIATALFLVVPLGGKLGDLREAAISF
                     IFKKPPRFVSLEADVEGVPNVVKAGQSLRIKGGETLIIRKITANTFFESYLTADVEGF
                     GRPNDVNEPIDTAEIRDRLMGTGVHSVPITVSYLDRVIAKVPLEISLTTQDFEDRLAA
                     AKTDKEKIAVLRSAHEAFPREETFLKRLTDLLSAQGDYASLIDIYKADSARDPQNTTL
                     LASLSLFYLKAGRLDEALDACKRIVDAGAADVEIYRRMAYIAGMKGDFDGRVSYLKKA
                     MELDPNNQDVVVDLAKTYEQAGFEGKAMEVYRGAGASARDREILVPLVRDALSRKDYR
                     EAKTLLERYVKQYPDDANALAQLAMVNGRLGETKSQIENYERAARISPDKAVLWYNLG
                     VSREKAGDHQGALEAYERVLGLAGGDTDALSGAARTALKLARYQQAKRYYEDLAKRQA
                     TRENLRGLVAASLGAKDEEAVIRACKAFLERYRDHDVAVILAGAYESRAASKQGRARL
                     DDLAKALDAYKTARAIDPSSKIASEKIPELSIAVIKLKKSASP"
     gene            6059..6709
                     /locus_tag="PLT69_00030"
     CDS             6059..6709
                     /locus_tag="PLT69_00030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ12867.1"
                     /translation="MRRMIAIGDIHGCLGKLEDLFRVIEPGADDRIVFLGDYVDRGDE
                     VFETVEFLVDCASRYDCVFLRGNHEDMFLQYLEFGGNERVFFANGGLRTIESYVGPDG
                     PASREQVQRSMPESHRRFYANLEWFHEEGGYLFVHAGLRPGVSLKDQDRRDLVWIRDE
                     FIARPTGLPLKVVFGHTPFSRPLVKPDKIGIDTGAVYGGPLTAVVLPEEKFLQSER"
     gene            6717..7388
                     /locus_tag="PLT69_00035"
     CDS             6717..7388
                     /locus_tag="PLT69_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ12868.1"
                     /translation="MAKTVSKDYYRTLGLSPDASTREIKKRYRELAMKFHPDRNPDDQ
                     EAAERFREITEAYGVLVDESRRRAYDARTQGAFDRAAVFEDIFANKDFREVFEGLPIS
                     RAWLERILWAGRIVAYEAIVVGGPPSAVLKRSAARLASEGARRMFHAVMDMHERLVLP
                     VADAGQGRTVTLEYRPGFTVRRIRVKIPPNTGQGTVLRIRGMGRRGPLGKAGDLYLHV
                     SIDGS"
     gene            complement(7358..8269)
                     /locus_tag="PLT69_00040"
     CDS             complement(7358..8269)
                     /locus_tag="PLT69_00040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ12869.1"
                     /translation="MSKEKNATRILIVDDEPETLDLIADFLVGGGFEVATATTGEEAL
                     TLVQKDLYHIAVVDLHLPGMTGSELLRKIKSSRNDVQVIMITGYGTIRDAVECMKLGA
                     SDFITKPILPDHLSLTIRRILEEARLKEEAELADYYRNLSRTDELTGLYNFRHLMTVL
                     KNEVSRHLRYNRVLSLGMIDIDDFKIFNDTKGHEEGNVLLGRLAHVFKHNTRNCDILA
                     RYGGEEFVILFPETSIEEASVVSARILATVEANLGITVTIGLASLPEHSSDASELIRM
                     ADTAMYWGKARGKNQVVLYEPSMDTCR"
     gene            8895..9728
                     /locus_tag="PLT69_00045"
     CDS             8895..9728
                     /locus_tag="PLT69_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ12870.1"
                     /translation="MTEKPLLWEMEQACPLGLVERHIEGFPTLDASRMRIFRMLQDET
                     AGHEEIERAVSCDPAISARVVKLANSPFYRHSDHHVGIAKAIRTIGLDMVKCIALSAA
                     VIDAFRSQTEVMGMLWAHSYAVAVVAGSLGQTRNERDVLFTGGLLHDLGRMVLLSADA
                     EGYPGLCKTSRLWPEPLQERERYGIDHCSAGGILAERWRFPPEVVQVIKAHHEPRERF
                     SATIGLIDFIASAEEAGMDGGTPDYERFAPACAEHAYKELVNTVRNRYRSNSAVVDNL
                     C"
     gene            9746..11857
                     /locus_tag="PLT69_00050"
     CDS             9746..11857
                     /locus_tag="PLT69_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ12871.1"
                     /translation="MNNLSDRMGGTPESVTDRERILLDLLGKTSGFYEQTIGILSSIF
                     EISQYVSEEDRSRDFFTYITDVLVSESRCENASIFMVEGDEIVLKAASGKTLQNANTD
                     LRIPLGKGVAGTCASRGRPIVVGDVEECEFFMRMDSARVNIGSMLCVPIKEGRSTVGV
                     MNLSHSSKGFFDVHSIRVFELLGLLIGNLFTLVHVSEVFRRRNVDLGHTLRAKEDSLR
                     ILTQRYKAVVDATEEMICIADTQGSVLFSNQALQRRHGSRPAHLAELFGDAAARTVIE
                     RARALHGGESADFELTVRTGSRDEMIGQFLLVPLEGGEILVVVRDVTARVKIEQKTRQ
                     TEKLTSLGLLTSGIAHELNNKLTPIMGFLELIDASGLSERDKKRLSIIVNSAQSAKGI
                     VESLLKFSRNRPPERTVFDMREVIQRAVTLYAPICKKRGIRIVAENPKGSLRVKADMN
                     CMEQVLVNFISNAIDAIGESQGTIWVRSALEDDCVSVCVEDTGPGIPEDIMDRIFDPF
                     FTTKANDKGTGLGLSICFGIVTEHRGDITVENTGGGARVTMKIPASCDLDEDLTPPEK
                     VHAADTLGFGEMQGLIMVVEDEEALLDLMVDTLSPHFHVMTFDNGRSAFEHLDEYSWE
                     LIISDLRMPVMNGMEFYEEAVRRNPSLKNRFMFITGDTYDYQVKEFLERNKVTYLRKP
                     FRISELKETVSRQLQWVKTTS"
     gene            11836..12549
                     /locus_tag="PLT69_00055"
     CDS             11836..12549
                     /locus_tag="PLT69_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ12872.1"
                     /translation="MGEDNELKKRILVVDDEEIVRYSLKNILAARGYEVDDVPSAERA
                     LELFREKTYHLVLTDLVLEGMGGLELLENVKVISPRTLVIVITGYGSLKTAIRALRLG
                     VYDYLLKPCEEEELVLRVKRALEMQNFGMEQQRLQELSAIAKTAVTLSDRINTPLSII
                     LGNIEMLQLKDGVGTRPDVRETLRLMEEQVFVIKRVMEKLANLTIAHTKKYPALMGCE
                     IIDLAREDSHGGGQEHPDR"
     gene            12521..12913
                     /locus_tag="PLT69_00060"
     CDS             12521..12913
                     /locus_tag="PLT69_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ12873.1"
                     /translation="MAGKSILIVDDSPEMVRMVAEYLNMHGYLVHTALDGDEALSCLG
                     TRDVEVVVSDIHMPRIDGFTLMEEIRTRYPNVPVILVTGYSVREAERIALERGANAFM
                     AKPFRLRDLKSVIDRVSSEADPYPAGRM"
     gene            12921..13379
                     /locus_tag="PLT69_00065"
     CDS             12921..13379
                     /locus_tag="PLT69_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ12874.1"
                     /translation="MKKSKVKKPQKILCYIDFAKSSAYVVEYAHKLACLLEAELFVLH
                     TVTDIRSAAGFYVPHINTDKLEEEVVKAAKDKMYAICSQVGDECVDARHRLVTRGVPI
                     EAINKVIKEKEIDLLVVGHEVSTGALKSFKGDQAEKFMKNPTIPFLVLPV"
     gene            complement(13424..13687)
                     /locus_tag="PLT69_00070"
     CDS             complement(13424..13687)
                     /locus_tag="PLT69_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12875.1"
                     /translation="MRDAHTMGFDTLLPLVILVLFFAVPSLFRFLARHSKTGLPRSVG
                     GPTEAPSARREDEPEGENPWRDHEAERHLSGEPDNRPIHPRWF"
     gene            complement(13752..14348)
                     /gene="yihA"
                     /locus_tag="PLT69_00075"
     CDS             complement(13752..14348)
                     /gene="yihA"
                     /locus_tag="PLT69_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOJ12876.1"
                     /translation="MKVRIRDASFSGVYPHVDMGLPQVAFAGRSNVGKSSLINALLNR
                     KNLVHTSKKPGKTRTVNFYRVESVELAPLYLVDLPGYGYAEAPKTVIEAWRQAISSYL
                     AGAAALRLLVLLVDIRRDIGDHERLLIQLASKTKAGLLVAATKGDKLGHGQRTLRVAE
                     LSRQAQAGVVLTSAHDRTGVDDLWDHIASAVRQEGRPR"
     gene            complement(14345..>14834)
                     /locus_tag="PLT69_00080"
     CDS             complement(14345..>14834)
                     /locus_tag="PLT69_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ12877.1"
                     /translation="DVITVNAARKTYAMTGWRLGWAIFPAHMIRSVQKLQQNLMICAP
                     SVAQWAGVAALTEAGPDVERMHRTFGRRRKVMLDEMARWRFNVEVEPTGAFYVLVNMR
                     KYTKDSRAFAMEILERTGVGVTPGIDFGSGAEGYIRFSYANSETNIVEGISRVARFLE
                     SV"
     assembly_gap    14835..14844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14845..15539)
                     /locus_tag="PLT69_00085"
     CDS             complement(<14845..15539)
                     /locus_tag="PLT69_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ12878.1"
                     /translation="MKPSSTSLDIQPFLVMDVMERANTLASAGRDVIHLEVGEPDFDT
                     PPCIRRAAWEAMEKGKTHYTDSLGIPELREAISGHYARTYGVKVDVDRIVVTAGSSPA
                     LLLAMACLIDAGDTVMMTDPYYACYPNFVKVLKGTPLFVPTGSADGFQLDPGKAHALI
                     KPSTRAIVVNSPANPTGVCLDAEHMKAIASLGVPVISDEIYHGLVYESEQHTILEFTD
                     EAVVINGFSKAYA"
     gene            complement(15728..15967)
                     /gene="xseB"
                     /locus_tag="PLT69_00090"
     CDS             complement(15728..15967)
                     /gene="xseB"
                     /locus_tag="PLT69_00090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOJ12879.1"
                     /translation="MKTRKKGKGYSEVLRELEETIERMGKGEVPIDELEETIRQASEK
                     IRFLRERLKATEAEVTRVLAEIEQGPSGSDGQKEP"
     gene            complement(15933..17420)
                     /gene="xseA"
                     /locus_tag="PLT69_00095"
     CDS             complement(15933..17420)
                     /gene="xseA"
                     /locus_tag="PLT69_00095"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOJ12880.1"
                     /translation="MSCLRVSEVNEYARQALAQRFGSPVWVVGEIHGLKTHAKSGHVY
                     FDLVEKGPDTNEAYIAKIACAFFQGSVAAWRRSLAANGFRDFDLASGMEVKLRAKVDL
                     FVREGRYQLIVSEVDPTYTLGAIARIRAKTIELLKASGLLEKNRSLQLGPVPLRIGLV
                     TSVDSAAYRDFTSIIQKSGFAVSVFVFDAHMQGDRAAGEVARGIRVLAAIEGLDVIAI
                     VRGGGAKTDLIAFDDIGICRAIATCPVPVITGIGHEIDLSVADMVAHRCLVTPTDAAR
                     FLVSRLEEAWSFLEGSASLLEKSSLQTIDMASRALELACTRLALYARTRTQRMRSALE
                     SAAARFTAGASSTAALHTERLLGLARSLGHRASGCLREHAALLDSRSALLAGATRHVL
                     YRGLAEFSATLDSRLRDLSQGIGVQLERLDRLERAANVLHPRHTLDRGYSLTLDSAGR
                     VCTDARHVGLGASITTVLAHGRLHSTVERKEHADDEDAKEGQRIL"
     gene            17531..>18102
                     /locus_tag="PLT69_00100"
     CDS             17531..>18102
                     /locus_tag="PLT69_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOJ12881.1"
                     /translation="MAYQFVKADITDAVAVVEMDRKHELNALSFGLMREIHHVFSSVL
                     HESSSVRVVVFTGGDECFSAGIDLKEAPYFSAEDAAEYFRLALELYTLLLDQDKIIIT
                     AVSGIAFGGGFNLALMGDMIIASESAIFAHPEIKYGFNPLLTPLVARVGMARARELAL
                     RGDPIGAREAYGIGLVNRVVAPEFFRDDVMG"
     assembly_gap    18103..18112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18113..18320
                     /locus_tag="PLT69_00105"
     CDS             <18113..18320
                     /locus_tag="PLT69_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12882.1"
                     /translation="AGQLVGRSPEVVKAVKRSLDVVSRLDAKAALEYELEMTSMLFNI
                     KGDIRERMKAVVKSKGHEDTSCKG"
     gene            18295..19032
                     /locus_tag="PLT69_00110"
     CDS             18295..19032
                     /locus_tag="PLT69_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOJ12883.1"
                     /translation="MRTLPARAETFPFDPAGLHAVLFAHYGPSHWWPGETPFEVMVGA
                     VLTQNTAWRNVEKAIANLKASSLLSVEGIHGLSPEELARLIRPAGYYNVKAARLKNLV
                     TLITRDFGASLERLFMLSDSDLRLALLGVNGIGRETADSICCYAANRLVFVVDAYTKR
                     ILSRHGVIGPGADYEEVRALFEGSLPRNLGVYKDLHAHLVYVGKDYCRAKRVRCGDCP
                     LGGPGCLPIPAGDGSLRPQAGAPGSPE"
     gene            19042..>19161
                     /locus_tag="PLT69_00115"
     CDS             19042..>19161
                     /locus_tag="PLT69_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ12884.1"
                     /translation="MEIFDVIVVGGGHAGVEAALASRRLGMDTALFTLSVDAIG"
BASE COUNT         3532 a         5811 c         6462 g         3326 t
ORIGIN      
        1 ctcttctttt ccatggccac cttccgccgg gccccgtctc ctcgccgggg gcctcctcag
       61 ctgcgatctt cccacggttg caacagcccc ggtctctgcg agacgggctc gatcgtcctc
      121 gccgggatga aggcacctgc cttcgagcga ggctttccgg gcgaaccggg atgtgccttg
      181 cgggtcacat gcgccagtag aggatcttgt tcgtctgggt cgtgtaggcg ctgtttgccc
      241 ggatggcacc ggtgttgtaa atgccgtcgt cgtacttcat gtcgatctgc tcggcggcgt
      301 cgtacgggat cgcgttgaac acgaccgcgt tggactggag tgccgcgttc cactggatct
      361 gtgcccagct cccgtacggg ttctggggac agctgttggc ggtcccgttg tagttgccgt
      421 tgatgatctg ggcgagaacc aagtcctcga agaggtacca gtactcgttg cccgtgatct
      481 gaccgtcctg gttgccgttg tgcgtgtcgt tgggcgggat gctcgcctgg ttctcgtcgc
      541 cggggtacac gttgtacctg tcgtagaagg cgtagtaggc ggcggtcagc gagtctttct
      601 ggttgacgag cttcttgatc ttggcgttct ggatgagggc ctctcccttg aggacgcccg
      661 tgatgagcag gcctatgatc acgagcacca gcgccagctc cacgagggtg aaacccctgg
      721 tatccttgcg tctgaacctt tccatgtggg ttcctcctgt tctctttttc attgccgggc
      781 ccaccctcgc agggggtgaa acctgcatga catatcggaa cgggggtgag cgcacttgaa
      841 tacacccgca cgagaagggg cttcccgccc tcctccaggt gcacagcgtc gccgcctgac
      901 cttcggacag tcatgcccac gggaaaggtc gggacgggct ctgcagtctc acaagacatc
      961 cgtggcatgc tgtatgtcgg gagggaaagg tggtgtgcat gcggggtgcc ctgtgagggt
     1021 ctcggcccat ggcatgggtg cgcggagcgg gagatgggag ggtgacccgc aaggtctccc
     1081 cgattgtcct tcccggccgg catccctggg acggggcgtg agcgctgatg cgtcggccct
     1141 taagggaccc cgcggatctt catgcccatg aagcggggtg tgaggagggg gagggacgag
     1201 acatcgatct cgaggggctg acccgagaac gagatgaggc gcaggagccc cgcgtctgca
     1261 tccaggctcg ggccgaacgc cacgccggtg cacagggtcg tatcctccag gattccgccc
     1321 gtggacgcgt cgagcacgac gagagcggag acgccctcgt ccgcgccgcg gataccgtct
     1381 tggacccgcc acacgaccag gtacaccttg gcatccatga cggtgacccc ggtgatgcgg
     1441 ccgggagcga tggatgcgga ccaggtcaag gctgctccag aaaagaggtg gaccctgtcg
     1501 ggggtgaccg cgagaacgtg ccttctgccg gaaagggtga aggacctgac gtctgtgacc
     1561 gcttcttcgt cacccgtggc aagcacgctc gaccacgaca gatcggcgtc gaaacccagg
     1621 acgagcccgt cggacgttcc ggcagccacc tcgcccgtta ccgggtcgta cctgacgacc
     1681 gtgatctcgt ggcctgcatc gatgctgcaa accggttctc cgtcgtcgat gcccacggcg
     1741 gtgagggaag ccccgtccgc cacgtagacc gtggatgccg atgcgtccca ggacgaggcc
     1801 ttccggctcc agatgcgtcc agatccccat gccacggacg aaaggtcgcc cgaggcgttc
     1861 gggcctgcga gcacccggtc tttcctgatc gagacgaccc ctcctgcgcc ttcgaagagc
     1921 cactcgatgc cgcctccggc gaggtcgatc aggacggcgg catccctcga ggtggtgcac
     1981 gcgagcatgc gtcccgagag cgcgacggaa cgggccgcgc ccctgagaac gatgtcttgg
     2041 caggcgaagc actcgccggg atcggccacg gaccgccgcg cccgtgacca cacgtcggcg
     2101 cccacccccg aaacgtcgag cctgcctccc gcgttcagga ggtggatcac cccgcctggg
     2161 tgaacgacgg gcgggaggct cgccgtgaca cccgcaggcg ccaggccttc gggtgtgggc
     2221 atgaacatgc ctcagccctt tgaggccgca gcccggagaa ggggtgtgtc gggaccgatc
     2281 acgtgcaccc tgacgccccg ggaccgtgcg tatgccgcga cgtcgtccag gcagtgggac
     2341 ccttcacctt cgacggcggt gtccatggga tccccgtccc tgtcgtagtc cctgagccag
     2401 ccgggcaagt ccccgtccga gagggggatc ccggccgtgg cgaccacgat atgcctgggt
     2461 tcgagcctgg gatcgttgaa cctgtcgatc gccctgaaga gcgcctccgc caggggagcc
     2521 tcaggcgccg tgggacaggc cgaggcgagg accctggtga tggggccggg agaaggggag
     2581 aagggggccc tcaagccgcc tgaggtgctc tccagcccga agcaggcatg atcgaggccg
     2641 gcaaggaggt ggtagacgtt cgagagggcg aacgtccagc acgacatggc gcccgtgcgg
     2701 gaggagccag tgggggcgcg ttcgtggctg ttcccgaaat cacccctgaa gaaggtgccg
     2761 gcggtggtcg caaggctcga tgcgatctcg ctgcagcctg agacacgcac gatctgtccc
     2821 gccgcgttct tgaggtagag tgcctggggc tcgtagtcga aacccgacga gatgcgcatg
     2881 agctgcgagt cgtccaggag caccaggatg ccaggagatc gggccgggac aagatccatg
     2941 ggaaggatgg gcgagcacgg ctggctctgc atgacgcgca tggagtatgc acaggcgcct
     3001 tcggtcctcg tctcgacgag taccgaggag aagtggatgt ggtcccaacc ctccggcgcg
     3061 cccaggaagg tggatgtccg cttcgcatcg tcaccttcac cggcgagcag ggtgcgtccg
     3121 aggatgtccg cgaaggcacc cgccgacttg aggtggacga gggccaggga ggcggcggcg
     3181 agggtcaggg ctatcgttgc ggtgaggaag acgaggcaga gcgcaaggat gaagccgcgc
     3241 tcacctgacg ggaaagcgga agtgcacgtg ttttgaaacc tcatgagagg cggcctccag
     3301 aaagatggag agatctcccc cgagtgcgcg gggttcgaac ctcgtgatcg cctcggcgag
     3361 aagctgggag ttcctgtaga gaccgtccgg cttgagctcg tagtggacgg cgggcacgat
     3421 ccgctcgccg gcgcgcacgt cggccggatg gccgatgggt acgcgcaggg aaccccgccg
     3481 gtacgaaccg gacacgacgc ccgccgacgt gtcggtgatg acccccagga aggcccagaa
     3541 gtccggcctg ccgtcactgt cgatatccac ggtgtcgagc gtcaaggccg ccccgccggt
     3601 ctcggcgatg acggcgtagt acggcggggc cttgaccggg tggacctgga tccccgggtg
     3661 ctgggaggtg acgggtatgc cggctatgaa cagaccgttg gacgtgaccc tgaacgccag
     3721 gctgcgaggc atgaggcacg cagcctgcgc caggtccgtg tgcagctcga gcgaggccgc
     3781 cctcagggac tgcatgcagg accaggtatc cctcgcggcc ttgtagaacg tggaggacac
     3841 gaggtacagg tggtacaggc cgatggtcac gagcgaggcg cacgcgagcg caaccatgag
     3901 ttccacgagg ctcatgccct tctccggtgg gtcggcaaaa aggcctccgg ggccctcggc
     3961 aacccggtcc gcgtgttcga ggggtctcgc gccctctcnn nnnnnnnngc agaaaggggt
     4021 ctgtgctcca aaggggggta agcgacgggt atgcatcaat ggagcgtcag gggagcatgg
     4081 cgtgccgcgg ttgcacgaga tcctgcggct tcgcgcggtg gactgccgtt cggcctcctc
     4141 tcaaggggca aggctcgctc cctcatgaca tgccagacac ccatctttac cacccgtccg
     4201 tcttggagtc aagcacaggg gaagatcgcg ggcaacggcg agcgaccggg gccgcgagcg
     4261 gcgggaaagc gttcccggga gaaggcggcg catgagggga aaaagcccgt ggacatgccg
     4321 gggagatctt gataatacat ccagccagtc ccctatgtcg cacaaaccgt cctatggaaa
     4381 cgccacgatc gtagtcatcg tggggatggc aatcgcaacg gcgctcttcc tcgtcgtgcc
     4441 gctgggcggc aagctcggcg atctcaggga ggcggccatc tcgttcatct tcaagaaacc
     4501 gccgcgtttc gtgtcgctcg aggcggacgt ggagggcgta ccgaacgtgg tcaaggccgg
     4561 gcagtcgctg aggatcaagg gcggcgagac gctcatcatc aggaagatca cggcgaacac
     4621 cttcttcgag tcctacctta cggccgacgt cgagggattc ggcaggccca acgacgtgaa
     4681 cgagcccata gacaccgccg agatcaggga ccgtctcatg ggcaccggcg tccactccgt
     4741 gccgatcacg gtctcttacc tggatcgtgt catagccaag gtcccgctcg agatctccct
     4801 tacgacgcag gacttcgagg acaggctcgc tgcagcgaag accgacaagg agaagatcgc
     4861 cgtcctgagg tccgcccacg aggccttccc aagggaggag accttcctca agaggctcac
     4921 ggacctcctt tccgcccagg gtgattatgc gtccctcatc gatatctaca aggcggactc
     4981 ggcccgggac ccgcaaaaca cgacgctgct ggcttccctg tcgctcttct acctgaaggc
     5041 gggcaggctc gacgaggccc tggacgcctg caaacggatc gtcgacgcag gagcagccga
     5101 cgtggagatc taccggagaa tggcctacat agccggcatg aagggtgatt tcgacggcag
     5161 ggtgagctac ctcaagaagg ccatggaact cgacccgaac aaccaggatg tggtggtcga
     5221 cctcgccaag acctacgagc aggccggctt cgagggcaag gccatggagg tgtacagggg
     5281 ggcgggcgcc tctgccaggg acagggagat cctcgttccg ctcgtgaggg acgcgctctc
     5341 gaggaaggat taccgcgagg ccaagaccct cctcgaacgg tacgtgaagc agtaccccga
     5401 cgacgcgaac gcgctggcac agctggccat ggtcaacggg aggctcggtg agacgaagtc
     5461 ccagatagag aactacgagc gcgccgcaag gatctctccc gacaaggcgg tgctctggta
     5521 caacctcggg gtgtccaggg agaaggccgg ggatcaccag ggggccctgg aggcctacga
     5581 gcgggtgctc ggccttgcgg gcggggacac ggacgccctc tcgggcgcgg cgcgcaccgc
     5641 cctcaagctc gcccgctacc agcaggccaa gaggtactac gaggacctgg cgaagagaca
     5701 ggccacgagg gaaaacctca ggggcctcgt ggcggcgtca ctgggggcca aggacgaaga
     5761 agcggtgatc agggcgtgca aggccttcct ggagaggtac agggaccacg acgtcgcggt
     5821 gatcctggcc ggtgcatacg agtcccgcgc tgcatccaag cagggccgtg cgaggctcga
     5881 cgacctcgcc aaggccctcg atgcgtacaa gacggcacgc gccatagacc cgagctcgaa
     5941 gattgcgtct gagaagatac cggagttgag catcgcggtg atcaagctga agaagtctgc
     6001 gtcaccgtga cggcaccctc gaggaaagaa cggggcgaaa gggaagcaac gacgatccat
     6061 gcgcaggatg atagccatag gggacatcca cggctgcctc ggcaagctcg aggacctgtt
     6121 cagggtgatc gaaccgggcg ctgatgaccg catcgtgttc ttgggcgact acgtggaccg
     6181 aggcgacgag gtgttcgaga cggtcgagtt cctcgtcgat tgtgcatcga ggtacgactg
     6241 cgtgtttctc aggggcaacc acgaggacat gttcctccag tacctcgagt tcggcgggaa
     6301 cgagagggtc ttcttcgcga acggcgggct caggacgatc gagtcctatg tcggccccga
     6361 cgggcccgcc tcgagggaac aggtgcagag gtccatgccc gagtcccacc gcaggttcta
     6421 tgcgaatctc gagtggttcc acgaggaggg gggctacctc ttcgtgcatg cgggcctccg
     6481 gccgggagtc tcccttaagg atcaggacag gagggacctg gtctggatca gggacgagtt
     6541 catcgcgcgc cccacgggcc tccccctcaa ggtcgtcttc ggccacaccc ccttttccag
     6601 gccgctggtg aagccggaca agatagggat agacaccggg gccgtctatg gggggccgct
     6661 caccgccgtc gtcctgccgg aggagaagtt cctgcagtcg gaaaggtagg ggccctgtgg
     6721 cgaaaacggt gtcgaaggac tattaccgga cgctcggcct ttcccccgac gcctcgaccc
     6781 gcgagatcaa gaagcgctac agggagctcg ccatgaagtt ccacccggac cgcaaccctg
     6841 acgaccagga ggcggccgag cgcttcaggg agatcaccga ggcctacggg gtgctcgtgg
     6901 acgagtccag gaggagggct tacgacgcgc gcacgcaggg ggcgttcgac cgggcggccg
     6961 tgttcgagga catcttcgcg aacaaagatt tcagggaggt cttcgagggg ctgcccataa
     7021 gcagggcctg gctggaacgg atcctgtggg cgggccgtat cgtggcctac gaggcgatcg
     7081 tggtaggggg gccgccgtcg gcggtcctca agcgttcggc ggcgaggctc gcctcggagg
     7141 gtgcgcggcg catgttccac gcggtgatgg acatgcacga gcgtctcgtc ttgcccgtgg
     7201 ccgacgcggg ccagggccgg acggtgaccc ttgagtaccg gccgggattc accgtgcggc
     7261 gtatcagggt gaagatcccc ccgaacaccg gtcagggcac cgtcctgagg atcaggggca
     7321 tgggcaggag ggggcccctg gggaaggccg gggatctcta cctgcacgtg tccatcgacg
     7381 gttcgtagag gaccacctga ttcttgccgc gcgccttacc ccagtacatg gcggtgtccg
     7441 ccatcctgat gagctccgag gcgtcgctcg agtgctcggg caggctcgcg agccctatgg
     7501 tgacggtgat gccgaggttg gcctccaccg tggccaggat gcgtgcggaa accaccgagg
     7561 cctcctcgat ggaggtctcg gggaagagga tcacgaactc ctcgccgccg tagcgggcca
     7621 ggatgtcgca gttgcgggta ttgtgcttga agacgtgcgc gagcctgccg aggagaacgt
     7681 ttccctcctc gtgacccttg gtgtcgttga agatcttgaa gtcgtcgatg tctatcatgc
     7741 ccagagagag cacccggttg tacctgaggt gcctggagac ctcgttcttg agcacggtca
     7801 tgaggtggcg gaagttgtac aggcccgtga gctcgtccgt gcgggagaga ttgcggtagt
     7861 aatccgcgag ctccgcctcc tccttcagcc tcgcctcctc gaggatcctc cggatggtaa
     7921 ggctcaggtg gtcggggagg atgggcttcg ttatgaaatc ggacgcaccg agtttcatgc
     7981 attcgaccgc gtccctgatg gtcccgtaac cggtgatcat gatgacctgg acatcgttcc
     8041 tcgaactctt gatcttcctg aggagctccg aacccgtcat gccggggagg tggaggtcga
     8101 ccacggcgat gtggtagagg tccttctgga cgagggtcag cgcctcctcg cccgtggtgg
     8161 ccgtggcgac ctcgaatcct ccgccgacga gaaagtcggc gatgagatcg agggtctccg
     8221 gctcgtcatc cacgatgagg atccttgtcg cgttcttctc ttttgacatc tctctcgaca
     8281 tcgaatggct cgaaggcggc gcctcccccc tttcgcctgc cataataacg tcggtgtctt
     8341 gcatggcaac tatctctctc aagtctctat aacggatagt cgggcaagaa tcatgaggga
     8401 tttatgaaga cgtggttcaa cgcggtggtc tccgccatgc cgggtggggc gggcacccgg
     8461 agcgcgcgga tgcgtaccag cgttcatgac ccttgcgagg atacggtctg gagcgatgcc
     8521 ggccgtgggt ctcgagcgat gtggcccgcg tcaccctcgg catgacccgc gcaggcgttc
     8581 ccgtcccttc ccgcgtgggc gagcgtccgc ccggtgattc ccgcgaccag gccgtgggtg
     8641 ccctttgccg accgtcgagg gtgagaacgc cgtcgtgtga ccacgggaca acccagctgg
     8701 agacgcctga ggtgtacgca aagcaaaagg cagtgagaac gccgtcgggt gaccgtggga
     8761 caacccaggg agcctttgac cgcctcgccc cgtccgggcg cggggatgcc cgcggatgcg
     8821 agaggtcttg ccgactggcg gtgcaccatg gcgagacgac gggagagagg acgctcggag
     8881 ggggctcaag aacggtgact gaaaaaccgt tattatggga aatggaacag gcttgccctc
     8941 ttggtctcgt cgagcgtcac atcgaggggt tccccaccct ggatgccagc cgcatgcgga
     9001 ttttccgcat gttgcaggac gagacggccg gacacgagga gatcgagcgg gcggtgtcgt
     9061 gcgacccggc tatctcggcc agggtggtca agctcgcgaa ctcacccttc taccggcact
     9121 cggaccatca cgtggggatc gccaaggcca tcaggaccat cggcctggac atggtgaagt
     9181 gcattgcgct gtctgcggcc gtgatcgatg cgttcaggtc acagaccgag gtgatgggga
     9241 tgctgtgggc gcactcctat gccgtggccg tggtggccgg gtctctcggt cagacaagga
     9301 acgagcggga tgtcctcttc acgggcggcc tgctccacga cctggggagg atggtcctgt
     9361 tgagcgcgga tgcagagggt taccccggtc tgtgcaaaac gtcccgcctt tggcccgagc
     9421 cccttcagga gcgcgaacgt tacggcatcg atcactgcag tgcagggggg atcctcgccg
     9481 agaggtggcg gttccccccc gaggtggtgc aggtgatcaa ggcgcaccac gaaccccggg
     9541 agcggttctc ggccacgatc gggctcatag acttcatagc ctcggcggag gaggcgggca
     9601 tggatggcgg gaccccggat tacgagcggt ttgcgccggc atgcgccgaa cacgcttata
     9661 aagaacttgt aaacacagtg cgcaacaggt atagatcgaa ctcagccgtt gtcgataacc
     9721 tgtgttgaga ggcattgggc gtgccgtgaa taacctgagc gatcgaatgg ggggcacacc
     9781 ggaatccgtc accgaccggg agcgtatcct tctcgatctg ctgggcaaga cctcgggctt
     9841 ctacgagcag accatcggca tcctctcttc gatcttcgag atctctcagt acgtctccga
     9901 ggaggacagg agccgggact tcttcacgta catcaccgat gtcctggtgt ccgagtctag
     9961 atgcgagaac gcgtccatct tcatggtgga gggtgacgag atcgtcctca aggcggcctc
    10021 aggcaagacc ctgcaaaacg cgaacaccga cctgaggatt cccctcggaa agggcgtggc
    10081 cggcacgtgc gcaagccggg gcaggcccat cgtcgtcggg gatgtcgagg agtgcgagtt
    10141 cttcatgagg atggactccg cgcgggtgaa catcggctcc atgctctgcg tgcccatcaa
    10201 ggagggcagg agcacggtgg gtgtcatgaa cctctcgcac tcttcgaagg ggttcttcga
    10261 cgtccattcc atcagggtgt tcgagctgtt gggactcctc atagggaacc tcttcaccct
    10321 cgtccacgtg tccgaggtgt tcaggcggag gaacgtggac ctcggccaca ccctgcgggc
    10381 caaggaagat tccttgagga tcctcacgca gaggtacaag gcggtggtgg acgccaccga
    10441 ggagatgatc tgcatcgccg acacgcaggg ctctgtcctt ttctcgaacc aggccctgca
    10501 gagaaggcac ggctccaggc cggcgcactt ggccgaactc ttcggagacg ctgccgcccg
    10561 gaccgtcatc gagagggcgc gcgccctgca cggcggcgaa tcagcggatt tcgagctgac
    10621 cgtgaggacg ggatccagag acgagatgat cggccagttt ctcctggtgc ccctcgaggg
    10681 aggcgagatc ctcgtcgtgg tgcgcgacgt gacggccagg gtgaagatcg agcagaagac
    10741 caggcagacc gagaagctga catcgctcgg cctgctcacc tccgggatag cgcacgagct
    10801 caacaacaag ctgaccccga tcatgggctt cctcgagctc atagacgcga gcgggctctc
    10861 ggagagggac aagaagaggc tctcgatcat cgtgaactcg gctcagtcgg ccaaggggat
    10921 cgtcgagtcc ctgctcaagt tctccaggaa caggcccccg gaaaggaccg tgttcgacat
    10981 gcgcgaggtg atccagaggg cggtcaccct gtatgccccc atctgcaaga agcgcggcat
    11041 acgcatcgtc gcagagaacc cgaaggggtc tctgcgcgtg aaggccgaca tgaactgcat
    11101 ggaacaggtt ctcgtgaact tcataagcaa cgcgatcgac gccataggcg aatcccaagg
    11161 caccatatgg gtgaggtcag cgctcgagga cgactgcgtc agcgtgtgcg tcgaggacac
    11221 gggcccgggt atcccggagg acatcatgga ccggatattc gacccgttct tcaccaccaa
    11281 ggcgaacgac aaggggacgg ggctcgggtt gtcgatatgc ttcgggatcg tcacggaaca
    11341 caggggtgac atcacggtcg agaacacggg cggcggagcc cgggtcacca tgaagatccc
    11401 cgcctcgtgc gatctcgatg aggacctcac ccctcccgag aaggtgcacg cggcggacac
    11461 gctcggtttc ggcgagatgc agggcctcat catggtggtg gaggacgagg aggccctcct
    11521 cgacctcatg gtcgacacgc tgagccccca cttccatgtc atgaccttcg acaacgggag
    11581 gtccgcgttc gagcacctcg acgagtattc ctgggagctc atcatctcgg acctcaggat
    11641 gcccgtgatg aacggcatgg aattctacga ggaggcggtg aggcgcaacc cttcgctcaa
    11701 gaacaggttc atgttcatca ccggtgacac gtacgactac caggtgaagg agttcctcga
    11761 acggaacaag gtcacctacc tcaggaagcc cttcaggata tccgagctga aggagacggt
    11821 ctcgaggcag ctgcaatggg tgaagacaac gagctgaaaa aaaggatcct cgtggtggac
    11881 gacgaggaga tcgtccgcta ctctctcaaa aacatccttg ccgcgcgggg atacgaggtt
    11941 gatgacgtcc ccagcgcaga gagggccctg gagctcttca gggaaaagac ctaccacctc
    12001 gtgctcacgg atctcgtgct cgagggcatg ggagggctcg agctcctgga gaacgtcaag
    12061 gtcatctccc ccaggacgct cgtgatcgtg atcacggggt acggttcgct caagacggcg
    12121 ataagggcgc tcaggctcgg ggtgtacgac tacctcctca agccgtgcga ggaggaagag
    12181 ctcgtgttga gggtgaagag ggccctggag atgcagaact tcggcatgga acagcaacgt
    12241 ctccaggagc tgagcgccat cgccaagacc gcggtcaccc tgtcggaccg catcaacacg
    12301 cctttgtcca tcatcctcgg gaacatcgag atgctccagc tcaaggacgg ggtggggacc
    12361 aggccggatg tcagggagac cctccgcctg atggaggaac aggtgttcgt cattaagagg
    12421 gtgatggaga aactggcgaa cctgaccatc gcccacacaa agaagtaccc tgcactcatg
    12481 ggttgtgaga taatcgacct cgcgagagag gactctcacg gtggcgggca agagcatcct
    12541 gatcgttgac gattccccgg agatggtccg gatggtggcc gagtacctga acatgcacgg
    12601 gtacctggtg cacacggccc tggacgggga cgaggcgctg tcgtgtctcg ggaccaggga
    12661 cgtggaggtg gtggtgagcg atatccacat gcccaggatc gacgggttca ccctgatgga
    12721 agagatacgc acgcgctacc ccaacgtgcc ggtgatcctc gtcacgggat actcggtccg
    12781 cgaggcggag cggatagcgc tggagcgcgg ggcgaacgcg ttcatggcga aaccgttcag
    12841 gctcagggac ctcaagagcg tgatagaccg ggtgtcctcg gaagcagacc catatcctgc
    12901 cgggaggatg tagagagacg atgaagaagt ccaaggtgaa aaaaccgcag aagatcctgt
    12961 gctacatcga tttcgccaag tccagcgcct acgtggtcga gtacgcccac aagctggcct
    13021 gcctgctcga ggccgagctc ttcgttctcc acaccgtcac ggacatacgg agcgctgcag
    13081 ggttctacgt gccgcacatc aacaccgaca agctggaaga ggaggtcgtc aaggcggcca
    13141 aggacaagat gtacgccatc tgcagccagg tcggcgacga gtgcgtcgac gcgagacacc
    13201 gcttggtcac caggggggtg cccatagagg cgatcaacaa ggtgatcaag gaaaaggaga
    13261 tcgatcttct cgtggtcggc cacgaggtct cgaccggggc gctcaagagc ttcaaggggg
    13321 accaggcgga aaagttcatg aagaacccca ccatcccgtt cctcgtgctg cccgtgtgac
    13381 cggcgggcgc cctctgcgcc ttgggggcgg gaaggcccgg ctctcagaac catctggggt
    13441 ggatgggcct gttgtctggc tctcccgaga ggtgccgttc ggcttcgtga tcccgccacg
    13501 ggttttcgcc ctccggctcg tcttccctcc gggctgacgg cgcttccgtg gggccgccga
    13561 ccgatctcgg caggcctgtc ttggaatgcc tcgccaggaa ccggaacaac gagggtacgg
    13621 cgaagaagag caccaggatg acgaggggca ggagcgtgtc gaaccccatg gtgtgcgcgt
    13681 ctctcatggc ggagggaatg atagcacaac ctgccgggcg atgaacaggg ggatcgggat
    13741 ccactccctg gtcatcgagg tcggccttcc tgccggaccg cagacgcgat gtggtcccag
    13801 aggtcatcca cccccgtgcg gtcgtgggcg gaggtgagca ccacgccggc ctgtgcctgc
    13861 ctcgagagtt cggcgactcg cagtgtcctt tgcccatggc ccagcttgtc gcccttggtc
    13921 gcggccacga ggagccccgc tttcgtcttc gaggcgagct gtatgagcag gcgctcgtgg
    13981 tccccgatgt cccggcggat gtccacgagg agcaccagga ggcggagggc ggcggcgccc
    14041 gccaggtagg aggagatcgc ctggcgccag gcttcgatga cggtcttcgg cgcctctgca
    14101 tagccgtaac cggggaggtc gacgaggtag aggggcgcaa gctcgacgga ttccacccgg
    14161 tagaagttga ccgtcctcgt cttgccgggc ttcttgctcg tgtgcaccag gtttttgcgg
    14221 ttgaggagcg cgttgatgag cgaggacttg ccgacgttgg acctgcccgc gaacgccacc
    14281 tggggcaacc ccatatcgac gtggggatac acgccggaaa acgatgcgtc cctgatcctc
    14341 accttcatac ggactcgagg aaccgtgcca cgcggctgat cccctccacg atgttcgtct
    14401 ccgagttggc gtagctgaac cggatgtacc cctctgcgcc gctgccgaag tcgatcccgg
    14461 gggtcacgcc cacacctgtg cgttcgagga tctccatggc gaaggccctg gaatccttcg
    14521 tgtacttccg catgttcacg agcacgtaga atgcgcctgt gggctcgacc tctacattga
    14581 accgccacct ggccatctcg tcgagcatga ccttcctgcg gcgaccgaat gtccggtgca
    14641 tccgttccac gtccgggccg gcttccgtca gcgccgcgac ccccgcccac tgggccacgg
    14701 acggcgcgca gatcatgagg ttctgctgga gcttctgcac ggagcggatc atgtgggcgg
    14761 ggaagatcgc ccagccgagc ctccagcccg tcatggcata agtcttgcgg gcagcgttta
    14821 ccgtgatgac gtccnnnnnn nnnnatggca taggccttgg agaagccgtt gatcaccacg
    14881 gcctcgtcgg tgaactcgag gatcgtgtgc tgctcagact cgtagacgag gccgtggtag
    14941 atctcgtccg agatcacggg gacgcccaag gacgcgatgg ccttcatgtg ctcggcatcc
    15001 aggcagacac ccgtggggtt cgcgggggag ttcacgacga tcgccctggt ggacggcttg
    15061 atgagggcgt gggccttgcc ggggtcgagc tggaacccgt ctgcgctgcc ggtgggcacg
    15121 aagaggggtg tgcccttgag caccttgacg aagttcgggt agcacgcgta gtacgggtcg
    15181 gtcatcatga ccgtgtcgcc ggcgtcgatg aggcatgcca tggccaggag gagcgccggg
    15241 gacgaccccg ccgtcaccac gatgcggtcc acgtccacct tgaccccgta cgtccgggcg
    15301 tagtgccccg agatcgcctc cctcagctcg ggtatgccca gggagtcggt gtagtgggtc
    15361 ttgccctttt ccatggcctc ccaggccgcc ctccggatgc atgggggggt gtcgaagtcc
    15421 ggctccccca cctccaggtg gatcacgtcc ctccccgccg aggccagggt gttcgccctc
    15481 tccatgacgt ccatgacgag gaagggctga atgtcgagag aggtggagga aggcttcatg
    15541 ccgtcggtat agcagagctc gcggatcgtg gtcaatcggg cggcgccggc ttccgttcgt
    15601 ggcgggagcc cctcgagcgg ggggtttcgg ggaaaaacag gcccaagcgg accgggatgt
    15661 ggcgggggct cctcgagcag gggggctccg gccgagatgc ggatcgtccc gggaaggatc
    15721 gccgagctca aggctctttc tggccgtcgg atcccgaagg tccctgctcg atctcggcca
    15781 gcaccctcgt cacctcagcc tcggtcgcct tcagtcgttc cctgaggaag cggatcttct
    15841 cggacgcctg cctgatcgtc tcctcgagct cgtcgatggg gacttcgccc ttgcccatcc
    15901 tctctatggt ttcctcgagc tctctgagga cctcagagta tcctttgccc ttctttcgcg
    15961 tcttcatcgt ctgcgtgttc ctttctctcg accgtcgaat gcagcctgcc gtgagcgagg
    16021 acggtcgtta tcgatgcacc cagcccgacg tgacgagcat ccgtgcatac ccttccggcg
    16081 ctgtcgaggg tcagggagta tcccctgtcg agcgtgtggc gcggatggag gacgttcgcc
    16141 gccctctcga gcctgtcgag gcgttccagc tgcaccccga tgccctgcga caggtccctg
    16201 aggcgggaat ccagggtcgc ggagaactcg gcgaggcccc ggtacaggac atgcctggtc
    16261 gcgcccgcga gcagggcgga cctcgagtcg agcaaggcgg cgtgttcgcg caggcacccc
    16321 gaagccctgt gcccgaggga tctcgcgagc cccaggagcc tttccgtgtg gagggccgcc
    16381 gttgaggatg caccggccgt gaaccgggcg gcggccgatt ccaaagcgga tcgcatgcgt
    16441 tgagtccgcg tcctggcgta cagggcgagc ctcgtgcagg cgagctcgag cgcgcgcgat
    16501 gccatgtcga tggtctgcaa ggaggacttc tcgaggaggc tcgccgagcc ctcgaggaac
    16561 gaccacgcct cctcgagccg agagacgagg aatctcgccg cgtccgtagg ggtgacgagg
    16621 catcggtgag cgaccatgtc ggccacggac aggtctatct cgtggccgat gccggtgatc
    16681 accgggaccg ggcaggtcgc tatggccctg cagatgccga tatcgtcgaa ggcgatgaga
    16741 tcggtcttcg cgccgccccc cctgacgatg gctatgacgt ccaggccctc gatggcggcc
    16801 aggaccctga tcccccgggc cacttccccg gcggccctgt cgccctgcat gtgggcgtcg
    16861 aagacgaaca cggacacggc gaacccgctc ttttggatga tgctcgtgaa gtcgcggtac
    16921 gcggccgagt ccaccgacgt gacgagacct atcctgagcg ggacgggacc cagctggagt
    16981 gacctgttct tctcgagaag gccggaggcc ttgagcagtt ctatggtctt ggcccggatc
    17041 ctggctatgg cgccgagggt gtaggtcggg tccacctcgc tcacgatgag ctggtatctc
    17101 ccttcgcgca cgaagaggtc gaccttggcc ctgagcttca cctccatgcc ggatgcgagg
    17161 tcgaagtccc gaaagccgtt tgccgcaagc gacctgcgcc atgccgcgac ggagccctgg
    17221 aaaaaggcac aggcgatctt ggcgatatag gcctcgttcg tgtccgggcc cttttccacc
    17281 aggtcgaagt agacgtggcc ggacttggcg tgggtcttga gcccgtggat ctcccccacc
    17341 acccagaccg gggacccgaa cctctgggcc agggcctgcc gggcgtactc gttcacctcg
    17401 ctgactcgaa ggcagctcac tcaagaatac cgccttacca gaaagagcga ggaactatca
    17461 agcattcccc gattgacagg gtgggtgcct ggttgtagat gtcgtacaac acgcatggga
    17521 gggaaccccg atggcctacc agttcgtcaa ggccgacata acggacgccg tcgcggtcgt
    17581 ggagatggac cgcaagcacg aactcaacgc gctctccttc gggttgatga gggagatcca
    17641 ccacgtgttc agctcggtgc tccacgagtc gtcttccgtg cgggtcgtcg tgttcacggg
    17701 aggcgacgag tgcttctcag ccgggatcga tctcaaggag gccccgtact tcagcgccga
    17761 ggatgcggcc gagtacttca ggctggccct cgagctgtac accctgctgc tcgaccagga
    17821 caagatcatc atcaccgcgg tcagcggcat agccttcggc ggcggtttca acctcgcgct
    17881 catgggcgac atgatcatag cgtcggagag cgccatcttc gcgcacccgg agatcaagta
    17941 cggcttcaat cccctgctca cccccctggt ggcgcgcgtt gggatggcac gagccaggga
    18001 actcgcgctc aggggcgatc cgataggcgc acgcgaggcc tacgggatcg ggctcgtcaa
    18061 cagggtggtg gcgcccgagt tcttcaggga cgacgtgatg ggnnnnnnnn nncgcgggcc
    18121 agctcgtagg caggtcgccg gaggtggtca aggccgtaaa gaggagcctc gacgtggtga
    18181 gcaggctcga cgcgaaggcg gcgctcgaat acgagctcga gatgacttcc atgctcttca
    18241 acatcaaggg tgacatcagg gagaggatga aggccgtcgt caagagcaag ggacatgagg
    18301 acacttcctg caagggctga gacgttcccg ttcgaccccg ccggactcca tgccgtcctt
    18361 ttcgcccact acgggccctc gcactggtgg cccggagaaa cccccttcga ggtgatggtc
    18421 ggcgccgtcc tcacccagaa caccgcttgg agaaacgtcg agaaggccat cgcgaacctc
    18481 aaggcctcgt ccctcctgag cgtcgagggc atccacggcc tgagcccgga agagctcgcg
    18541 cggctcatca ggccggctgg ttactacaac gtcaaggccg cgcgcctgaa gaacctggtc
    18601 accctgatca cgagggattt cggcgcctcg ctcgagaggc tcttcatgct cagcgactcc
    18661 gacctgcgcc ttgcgctcct gggggtgaac gggataggca gggagaccgc ggacagcata
    18721 tgctgctatg cggcaaaccg gctcgtgttc gtcgtggacg cgtacaccaa gaggatcctc
    18781 tcgcggcacg gcgtcatagg gcctggtgcg gactacgagg aagtcagggc cctcttcgag
    18841 ggctcgctgc ccaggaacct gggggtgtac aaggatctcc acgcgcacct cgtgtacgtg
    18901 ggcaaggatt actgcagggc gaaaagggtg cggtgcggcg attgcccgct cggtggacca
    18961 ggctgtctcc cgatcccggc aggagatgga agccttcgcc cccaggcggg cgcaccggga
    19021 agccctgagt gagggtgggt cgtggagatc ttcgacgtca tcgtggtcgg cggggggcac
    19081 gccggggtcg aggccgcact cgcctcgcgc aggctcggca tggacacggc gctcttcacc
    19141 ctgtccgtgg acgccatcgg g
//
LOCUS       DAOSCR010000002        18866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_2039_length_18866_cov_3.074371, whole genome shotgun sequence.
ACCESSION   DAOSCR010000002 DAOSCR010000000
VERSION     DAOSCR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_18866_cov_3.074371"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1614
                     /locus_tag="PLT69_00120"
     CDS             121..1614
                     /locus_tag="PLT69_00120"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOJ12885.1"
                     /translation="MAGRLTRYALCVLAAVFLCVSCTRDAGQEKKAPSPGAAPSGGLI
                     KQETERSSSLAKGEIDFRNVIVEVARKNIPAVVHIEVTQSQEIANPFSPFENDPFFRR
                     FFNFPEGPRKFKRELKGIGTGMIISEDGYILTNNHVVAGASQLQVILSNGRQFGAKIV
                     GADPKTDLAVIRINADEKLPTVVFGDSDNVQVGEWVVAIGHPRGLDQTVTQGIISAKH
                     RKGITDPDTYQDFIQTDAAINPGNSGGPLLNLDGQVIGVNAVIASQSGGSEGLGFAIP
                     SNMAVHIARSLIATGKVQRGWLGVSIQDIPFEKMKELKLDSPKGVYVADVLSGGPAHK
                     AGVRPEDVILSVDGKEVADSAELRNLIASTPVGKVVRLGILRSGKRITLDVKVGNLED
                     SVKFMAVGLREKLGIDVRPVSDKERDRYGLDKDQGVAISWVDPKGPLGKAGFEVGDLI
                     LGVEGRPVEGVEDFVSLAGAIKKGQHVSVYALDHRTGRTGTIEVEVR"
     gene            complement(1645..4737)
                     /locus_tag="PLT69_00125"
     CDS             complement(1645..4737)
                     /locus_tag="PLT69_00125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOJ12886.1"
                     /translation="MADLQNRHWQALALLRAVGEPVESDLVSALVPLRQAELQGFLRA
                     GFDAGLVLVHSDGRIALARDLPHEVVRKLNRITTREHLLTMIEKIRRLGLQESLSGRA
                     FVSLLAKAGRDHDAAVFAAESARRSVRAGDKREALDLLETAHALAAPHIGMPEWDKLF
                     LDTTNELCRLRLNLTRDIHGIPAILEQAYPVAERIGDLRTLARCDLVTGLYRYIIGRT
                     AEGLGLIASGLDQARALGDEDIMAVSAEFRGVYYYLQGMYKEAVDSFEVVVRLGAKAP
                     TFLPEHLASHSALGYCSALLGQYHRAVGVLDSHWRRSKMAGNDRNTCFFEALLGIVLI
                     IMGRRQEAHEHLLGALREAEDGGNIPALHVVQKGLAYYSYFEGDLEEAYRITKDTVYA
                     EAIGPQYNWPVTLEMLYAFQAKGYPPIPGFGFEEEMERVLSGPNVHLRGVALRLRALQ
                     ARSRGDGPEVVLPLLEASESDLLRTGDTIELAKTRSEMARVKLDQGDRAAARNLALMA
                     WEGLGGYGRELFPDDLAPLLGTGAAGRALPGRKEFLDRFMDLMDEFVPSTDREELLTR
                     LVSAAGRFFGAERGGLFWFTGPRETARPVLRASFNLDRSEVFSEEFRSTLGLVFKAFR
                     NGEPLVLRPGGVTRGTAKHQAVAVICLPVVVSSEVAGVLYLDNTYVDPAGEVIDRDVV
                     VRVARHIGVSIERILHFTGMMDAYRSRLSTARTSEGEGSAPGAILGQSPVMTALLAQA
                     DQAAGTDATVLITGETGSGKELLARRIHDMGRRKAGPFVVVDLAAVPETLVESELFGH
                     EKGAFTGAERQKQGKVELAHTGTLFIDEIGDVPPSVQVKLLRVLQEKSFTRVGGTRAM
                     TSDFRLVAATNRDLKKDMEEGRFRQDLYYRLNVVPLALPPLRQRGSDVVLLAQEFLDQ
                     YARKYHRTLPGLTEEDIRAITAYPWPGNVRELKNVMERTAILSSPDRLLLSLPVSHGE
                     DPESPFADRPTMEELQRRYIKHVLGLTGGRIGGPGGAARILGMKRTTLQARMRKLGIA
                     "
     gene            4947..6305
                     /locus_tag="PLT69_00130"
     CDS             4947..6305
                     /locus_tag="PLT69_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ12887.1"
                     /translation="MAEQSSQKGWRDEYKNKLVTAEEAAAQVQSGDWIVVPPVNSAPI
                     DILNALAARKDEIQKVKIASNLLIYPFAFLGGDYVGKITYYSGYCGPLERMFMPQGNV
                     VPFPMHLSKAYKALDRAKAHDVAMIEVTPPDDRGYMNFGPCGVAYGRYATKKCRKVIA
                     QVNSKTPWVHGIENVVHVSEVDFIVEKDHDLPVVPEIEITDVEKKIGEHIAERIEDGA
                     TIQLGIGGIPNAVAYFLHEKKDLGVHAEILSDAIAELAELGVITGRKKTIFRDKIAVG
                     GLIIGSKRLLDFCDDNPTILSMPIQIANHYDTIRRHDNLCSINSALTVDLTGQIASET
                     IGHLQYSATGGQADFVRGALASKGGKSFIALPATGRNKDGSLMSRIVLNFAPGTVVTT
                     PRSDAMYIVTEYGVADLYMRSIPDRVKAMISIAHPDFREDLERQAFEAGLLIPATLPV
                     NV"
     gene            6476..8191
                     /locus_tag="PLT69_00135"
     CDS             6476..8191
                     /locus_tag="PLT69_00135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ12888.1"
                     /translation="MNYLIQPKKFKSLMADEGSRAIMEKTIAFFENMGKQRLLDDYHR
                     AIWYREFIDFIGRERIFFKLLTPSQYADGDPDARWDTARNSEYSELLAFYGLGYWYCF
                     QVTILGLGPIWMSPNEKAKRKAAKLLREGAIFAFGLSERAHGADIYSTETALTPKGDG
                     TYVANGEKYYIGNGNEAEMVSTLGKVKDGTDDYVFFVTNYRHKAYQLKKNVINHQEYV
                     SNFALNDYPVTEDDILSRGPHAWDCALNTVNIGKFNIGPASIGLAEHCFYEAITHASN
                     RILYGMRVTDMPHVRKNFMDAWLRLVAMKMYQRRATDYFRNASPKDRRYLLYNPTSKM
                     KVTLQSEDVVNLLWDVIAAKGFEKDTYFHMAASDMRGPSKLEGTVHVNVQLIRKFMKN
                     YFFNPAEYAPVPPDFSLKDDMFLFDQGPTKGLGSIQFHDYKPVFDAFKSLPNVGAFMK
                     QTEIFKEMLEKAGPDKAQDMDPSWSLPLGEMFSIVVYGQLILEQAQIDKVEDDVVNQI
                     FDFMVRDFAHFALQIYGNHASTDEQRGYCTKIMLIKAVPAPEQYQRVWEKYVSALNGE
                     YEMNP"
     gene            8619..8867
                     /locus_tag="PLT69_00140"
     CDS             8619..8867
                     /locus_tag="PLT69_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12889.1"
                     /translation="MGNVINIIRREGGDQALRKADAAKERKEIEDVVSIENRQASSPE
                     VKSVDEARDLLASVVEALKHAPTDLYALDVNRVSVMIS"
     gene            complement(9225..10340)
                     /locus_tag="PLT69_00145"
     CDS             complement(9225..10340)
                     /locus_tag="PLT69_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ12890.1"
                     /translation="MMVMSAFILVVVVLAVGVLVWIVADTKRSQPPAKPTRKAPARYV
                     SVSPQEDIGSFLEVYNQEWKPDRDALSHAPESVLELVEKIEQISPLITELSSKLNDPD
                     INPREISKIIVQDQGLTSFILRRVNSPYYGLLQKVDNIFNAIVILGYNEIYRIVMEER
                     TRKIGIRQDKADWVHANVTSTIAAYLTGVSRIGVPGGTMVTLGMLHDIALTVMAASLP
                     APPGGFSKDPRERLRQETEHYGIDHATLGGLLAKHWGMPQRLVACIAAHHWPMFWPVR
                     ELAKAHGDIVKELSILAISDVAARAFTQEISGPYIGDDYYRFVNRPPRVEDILTPEIT
                     RDIKRIYRVMLDDTLEHGEEPETSQTTRSAESTPAKD"
     gene            10864..11763
                     /locus_tag="PLT69_00150"
     CDS             10864..11763
                     /locus_tag="PLT69_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ12891.1"
                     /translation="MVEHVETPSQREKILAILERAPDILTLPGIIQQILEVTSRKNSS
                     ASDLTAIIESDPALTSRVIAVANSPYYGFVKKITNVSHAIVVLGFREIQNIALSMSVL
                     KMFDPKGSEFVDQLWKHSFCVGVASRMIAEHLRLKVEGKYFVGGLLHDVGKIFFSQYV
                     FTSFDRLLSVLWEEDTMESYHALEERMLGITHQEVARILLGEWMFPAEIVDAVACHHD
                     PASSEVDRLFACCIHLADVICTAKGITPLKDRHFVLIDRGVLRFLEECGHTLTTSDVK
                     SFMDRLDLEIERQSTYLSAFRNR"
     gene            complement(12085..13281)
                     /locus_tag="PLT69_00155"
     CDS             complement(12085..13281)
                     /locus_tag="PLT69_00155"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOJ12892.1"
                     /translation="MKRIILLGDGMADEPLPELGSKTPLECARIPNMDRLSVSGELGM
                     VRTVKEGFPPGSDVANMAVMGLDPSRYYTGRAPIEAVSMGVVLGEGDTAFRINLVTIS
                     RRDGTLFMEDYCSDHISTQEAGALIEDLKSIVQDMPGASMHPGIEYRHLLVLRGYDNP
                     GLKTTPPHDITGEPVDAYLPNDELLMEIIRRSMALLENHAVNRSRLSRGLRPATALWP
                     WGEGKAMRVPTLEQEYGITGAMISAVDLLKGLGKLRGMEVIEVPGATGWLDTNYEGKA
                     SSALEALGRHDLVYVHVEAPDEAGHSGSVEKKIQAIEDFDARIVGPVLEGLGRLGSPF
                     RLLVMPDHPTPICKKTHTADPVPYILYDSGAPGKTPGRRFTEAHARATGVFVEEGSSM
                     LARLIG"
     gene            complement(13312..14637)
                     /locus_tag="PLT69_00160"
     CDS             complement(13312..14637)
                     /locus_tag="PLT69_00160"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ12893.1"
                     /translation="METIRIGLIGLGTIGTGVARTLMENRGLLEDRLGFSLSLKGIAD
                     IDITRDRGIAIPQGLLTTDAMRLVEDPDIDIIIELVGGIEPAKTFILKALERGKHVVT
                     ANKALLATHGKEIFEAAQTFGRDLYYEAAVGGGIPIIKTLREALVANNYAFIYGILNG
                     TCNYILTEMGEKGSSFDEALSSAQKLGYAESDPTLDIEGIDAAHKLALVTSLAYGVPP
                     DMNGVYVEGITRLDKMDVTFARDLGYKVKLLALAIDRGDSVEARVHPTLVPTNYLLSK
                     VDGVFNAFYVRGDIVDSTLFYGRGAGMMPTASAVVSDLADIGRNILSGTAGRIPHLGY
                     RKGSSSPKRYLPIDDVVTNYYLRINALDRPGVLAKVAGILGERGISIVSVIQKGTDSV
                     DFVPVVMLTHEAREAGVKSAVEAISSLSEVNGKPVVIRVEDKKLKNSPS"
     gene            complement(14619..15632)
                     /gene="lipA"
                     /locus_tag="PLT69_00165"
     CDS             complement(14619..15632)
                     /gene="lipA"
                     /locus_tag="PLT69_00165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOJ12894.1"
                     /translation="MGEHGTPDVMPSCASRAPVPGEDTQEKTVEGRGSSSRLPAWVHV
                     RRDHGCLHEMKALLRGCGLSTVCEEARCPNASLCFANKTATFLILGDVCTRACRFCGV
                     AKGVPAPVDPTEPARVARAARMLGLEHVVITSVTRDDLEDKGARAFARTIRAIKDSIP
                     ACTVEVLTPDFGGLRDPLSLVLSERPDVFGHNVETVGRLQEKVRPGADLGRSLGLLEA
                     ARELAPHTVVKSGFMVGLGERADEIEDLLRSLAGAGCDIVTIGQYLRPSMRQVEVRRY
                     WEPEAFETWAGLAKSLGIGYCVAGPLVRSSFRARETLDDIRTARGSGDRSPTKEKVWR
                     RSG"
     gene            complement(15642..15926)
                     /locus_tag="PLT69_00170"
     CDS             complement(15642..15926)
                     /locus_tag="PLT69_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HOJ12895.1"
                     /translation="MRVIKALAFVALAVIGVLFAASNQEPATVHLYSFKTLTYPLYLV
                     LFGCLAAGTAVSILSALAASNDGKEIKALSRRRDELRERLKKSGGSGGQR"
     gene            complement(15923..16390)
                     /locus_tag="PLT69_00175"
     CDS             complement(15923..16390)
                     /locus_tag="PLT69_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOJ12896.1"
                     /translation="MKNLWAPWRMEYILSDKDGGCVLCEAPRREDGLILTSGATCYAI
                     MNRYPYTTGHTMVVPFRHVADICSLTPEESAELMAVSQKIVEATRCALKPDGFNLGIN
                     IGEVAGAGIAGHLHLHVVPRWEGDTNFMPAVGHTRVISEHIEQTHKKIREALS"
     gene            complement(16387..16875)
                     /locus_tag="PLT69_00180"
     CDS             complement(16387..16875)
                     /locus_tag="PLT69_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ12897.1"
                     /translation="MTRIGVVSDTHLRARERILEEIASMHFKDVDLVLHAGDIVCMDV
                     LGVFEEAGHTVVAVCGNMDGQDVRAKLPMSRTLAVEGVTIGLIHGWGSPEGIRSRLRR
                     ALPDAGVIVYGHTHQAYAGTQDGTYFFNPGSPTDNRFTTMRSVGIITVDSERVEGTII
                     EL"
     gene            complement(16944..18200)
                     /locus_tag="PLT69_00185"
     CDS             complement(16944..18200)
                     /locus_tag="PLT69_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ12898.1"
                     /translation="MDHRIAVIGLGYVGLPLAVEFGRRYPTLGFDINPRRIEELRSGM
                     DRTREVSPAELAEARLLDYTFDASDLERCNTYVVTVPTPIDAYNRPDLSLLMDACNTV
                     GRALGKGDIVIFESTVYPGCTEEECVPALERASGLTYNEDFFCGYSPERINPGDRRHR
                     LTDIVKVTSGSTPRTARIVDELYRSIIRAGTHPAPSIKVAEAAKVIENAQRDINIAFV
                     NELALIFARMGIKTSDVLAAASTKWNFLSFSPGLVGGHCIGVDPYYLTHKAEGLGYIP
                     QVILAGRRINDSMGRYVATELIKMMVKRKHRITDSRVLVLGVTFKENCPDIRNSKVMD
                     IITELREFECTVDVFDPVADPQDVRDAYGIEIMASEDEIQGTYEGIVAAVAHDAFRDM
                     DLSRLKAPGCVVYDVKSIYPDADAYL"
     gene            18509..>18866
                     /locus_tag="PLT69_00190"
     CDS             18509..>18866
                     /locus_tag="PLT69_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOJ12899.1"
                     /translation="MNGHISRIGVFDSGIGGLTVLKCLLDLVPAREMIYLGDTARLPY
                     GTKSPRTIVRYSLQCGRFLAEKGVDMLVVACNTASAHAVPALREELHIPVVGVVEAGS
                     RAAVETGGRKIGVIGTS"
BASE COUNT         3290 a         5999 c         6252 g         3325 t
ORIGIN      
        1 aacgatactc cggtgtatac accctcgaac cgtggctgcc ccgggcggtg ccgcgggcaa
       61 gagccccttg cgtgggagcg agttcgtgct attatgggtg gcccggacaa ggaggtgtac
      121 atggctggta ggctcactcg ttacgcgctc tgcgtgctgg cggcggtctt tttgtgcgtc
      181 tcgtgcaccc gggacgcagg gcaggagaaa aaggcgcctt cgcctggggc ggccccctcg
      241 ggcggcctca tcaaacagga gacggaacgc tcttcctccc tcgccaaggg agagatcgac
      301 ttcaggaacg tgatcgtcga ggtggccagg aagaacatcc ctgccgtggt gcacattgag
      361 gtgacgcaga gccaggagat cgcgaacccc ttctcgccct tcgagaacga ccccttcttc
      421 cgccggttct tcaacttccc cgaaggcccg cgcaagttca agagggagct caaggggatc
      481 gggacgggga tgatcatcag cgaggacggg tacatcctca cgaacaacca cgtggtggcc
      541 ggggcgagcc agctccaggt catcctctcg aacggcaggc agttcggcgc gaagatcgtg
      601 ggggcagacc ccaagacaga cctcgcggtg atcaggatca acgcggacga aaagctcccc
      661 acggtcgtgt tcggggactc ggacaacgtc caggtgggtg agtgggtggt ggccatcggg
      721 cacccgcggg ggctcgacca gaccgtgacc cagggcatca tcagcgccaa gcaccgcaag
      781 gggatcaccg acccggacac ctaccaggac ttcatccaga cggacgcggc catcaacccc
      841 ggcaacagcg gcggccccct gctgaacctc gacgggcagg tcataggcgt gaacgccgtg
      901 atcgcctcgc agtccggcgg atcggagggc ctcggcttcg ccatcccgag caacatggcc
      961 gtgcacatag cccgttcgct catagccacg ggcaaggtcc agcgtgggtg gctcggggtc
     1021 tcgatccagg acatcccctt cgagaagatg aaggagctca agctcgacag ccccaagggc
     1081 gtctacgtgg cggacgtgct cagcggcggg ccggcgcaca aggccggtgt caggcccgag
     1141 gacgtgatcc tgtcggtcga cggcaaggag gtggcggact cggccgagct caggaacctc
     1201 atcgcgtcca cgcccgtggg aaaggtcgtc aggcttggga tcctgcgttc cggcaagagg
     1261 atcacgctcg acgtcaaggt ggggaacctc gaggactcgg tcaagttcat ggccgtgggg
     1321 ctgagggaga agctcgggat tgacgtgagg ccggtgtccg acaaggagcg tgacaggtac
     1381 ggcctggaca aggaccaggg cgtggcgatc agctgggtgg acccgaaggg gccgctcgga
     1441 aaggccggtt tcgaggtcgg cgacctcata ctgggcgtcg agggcaggcc ggtcgagggc
     1501 gtggaggact tcgtctccct cgcgggcgcc atcaagaagg ggcagcacgt gtcggtctac
     1561 gccctggatc accgcacagg caggacgggg accatcgagg tggaggtgcg ctaggctccc
     1621 cctgcgcgtg cacggggatg cccctcaggc gatcccgagc ttcctcatcc tcgcctgcag
     1681 cgtggtgcgc ttcatgccca ggatccgggc cgcgcccccg gggccgccga tgcgcccgcc
     1741 ggtgaggccg agcacgtgct tgatgtagcg gcgctggagt tcctccatgg tgggcctgtc
     1801 cgcgaacggg gactcggggt cctcgccatg ggagacgggc aggctgagca ggaggcgatc
     1861 cggcgaggag aggatggcgg tcctctccat cacgttcttg agctcccgga cgtttcccgg
     1921 ccacgggtag gccgtgatcg cccggatgtc ctcctccgtg aggccgggca gggtgcggtg
     1981 gtatttcctg gcgtactggt ccaggaactc ctgcgccaag agcaccacgt ccgatccccg
     2041 ctggcgcaga gggggcaggg ccaggggcac cacgttgagc cggtagtaga ggtcctggcg
     2101 gaagcggcct tcctccatgt ccttcttcag gtcgcggttg gtggcggcca cgagccggaa
     2161 gtcggaggtc atggcgcggg tgccgcccac gcgggtgaag ctcttctcct ggagcacccg
     2221 caacagcttc acctggaccg aggggggcac gtcgccgatc tcgtcgatga agagcgtccc
     2281 ggtgtgcgcg agctcgacct tgccctgctt ctgcctttcc gcgcccgtga acgcgccctt
     2341 ctcgtggccg aagagctcgc tctccaccag ggtttcaggc acggcggcca ggtccaccac
     2401 cacgaaggga ccggccttgc gcctgcccat gtcgtggatc ctgcgggcca ggagctcctt
     2461 gccggagccc gtctccccgg tgatgagcac cgtggcgtcc gtgccggcgg cctggtcggc
     2521 ctgggcgagc agcgcggtca tgaccgggct ctgcccgagg atggcccccg gtgcggaccc
     2581 ttcgccctcc gaggtcctgg ccgtagagag ccgggacctg taggcgtcca tcatgccggt
     2641 gaagtggagg atgcgctcga tggacacccc gatgtggcgg gccaccctga ccaccacgtc
     2701 ccggtcgatg acctcgcccg ccgggtcgac gtaggtgttg tcgaggtaca ggaccccagc
     2761 cacctcagag gagaccacga cgggcaggca gatgacggcc acggcctggt gcttcgcggt
     2821 tccgcgggtg acgccgcccg ggcggagcac caggggctcg ccgttgcgga aggccttgaa
     2881 cacgagcccc agagtggagc ggaactcctc ggagaagacc tccgagcggt cgaggttgaa
     2941 cgatgccctc aggaccggcc gggccgtctc cctcggcccg gtgaaccaga acaggcctcc
     3001 gcgctcggcg ccgaagaacc tgcccgcggc cgatacgagg cgggtgagga gctcctcgcg
     3061 gtcggtgctg ggcacgaact cgtccatgag gtccatgaag cggtccagga actccttcct
     3121 cccgggcagg gccctgcccg cggcccctgt ccccagcagg ggcgcgagat cgtccgggaa
     3181 gagctccctg ccgtagccgc cgaggccctc ccaggccatg agtgcgaggt tccgggccgc
     3241 cgcgcggtcg ccctggtcga gcttcacgcg ggccatctcg gagcgggtct tggcaagctc
     3301 gatcgtgtcg ccggtcctga gcaggtccga ctcgctcgcc tccaggagcg ggagcaccac
     3361 ctccgggccg tcgcccctgg agcgggcctg gagcgcgcgc aggcgcagcg ccaccccccg
     3421 caggtggacg ttgggccctg acaggaccct ttccatctcc tcctcgaaac cgaagccggg
     3481 aatgggcggg tagcctttcg cctggaaggc gtagagcatc tccagggtga cgggccagtt
     3541 gtactggggc ccgatggcct cggcgtagac cgtgtccttc gtgatccggt acgcctcctc
     3601 aaggtcgccc tcgaagtagc tgtagtaggc gagccccttc tggaccacgt gcagggcggg
     3661 gatgttgccg ccgtcctcgg cttctctcaa ggcgccgagg aggtgctcgt gcgcctcctg
     3721 cctcctcccc atgatgatga gcacgatgcc gagcagggcc tcgaagaaac aggtgttccg
     3781 gtcgttcccg gccatcttcg acctgcgcca gtgggagtcg agcacgccca cggcgcggtg
     3841 gtactggccc agcagggctg agcagtagcc cagggccgag tgcgaggcca ggtgctcggg
     3901 gaggaaggtg ggcgccttgg cgccgagccg caccacgacc tcgaagctgt ccacggcctc
     3961 cttgtacatg ccctggaggt agtagtagac gcccctgaac tcggcagaga cggccatgat
     4021 gtcttcgtct cccagggccc ttgcctggtc caggcccgag gcgatgagcc cgagcccctc
     4081 ggccgtgcgg ccgatgatgt accggtacag gcccgtgacc aggtcgcagc gggcgagcgt
     4141 ccgcaggtcg ccgatccgtt cggccacggg gtaggcctgc tcgaggatgg cggggatccc
     4201 gtggatgtcg cgggtcaggt tgagccgcag gcggcacagc tcgttggtcg tgtcgagaaa
     4261 gagcttgtcc cactcgggca tgccgatgtg aggggctgcc agtgcgtggg cggtctccag
     4321 gaggtccagg gcctcccgct tgtcccccgc acggacggac ctccgggccg actccgcggc
     4381 gaacacggcg gcgtcgtggt cacggcccgc ctttgccagg agcgagacga aggcgcggcc
     4441 gctcaggctc tcctggaggc cgagcctgcg tatcttctcg atcatggtca agaggtgctc
     4501 cctggtggtg atgcggttca gcttccggac gacctcgtgg ggcaggtccc gtgccagggc
     4561 gatgcggccg tcggaatgga cgagcacgag accggcgtcg aagccggcgc gcaggaaccc
     4621 ctgcagctcc gcctgtcgca gcgggacaag ggccgacacg aggtcggact ccaccggctc
     4681 cccgacggcc ctgagcagcg ccagggcctg ccagtgcctg ttctgcagat ccgccatgat
     4741 gcacctcctt tgggcgttca cggagtgtag gcccctttgc cgcaggaggc aagacgatat
     4801 ttgggcggac agtgacgaca tatgggcagt tccgctgcat cccaactgaa atctcccatg
     4861 aaatgaatag cgtgagaatg ccccgggtgt ggcacgcctt ctgctcgtac cccggctgac
     4921 caccacatac aaccggggag gacgccatgg cggaacagtc gtcgcagaag ggatggaggg
     4981 atgagtacaa aaacaagctc gtgacggcgg aagaggccgc ggcccaggta cagtcaggcg
     5041 actggatcgt ggtcccgccg gtcaacagcg cgcccataga catcctgaac gcgcttgctg
     5101 cccgcaagga cgagatacag aaggtgaaga tcgccagcaa cctcctgata tacccctttg
     5161 cgttcctggg aggggactat gtcggcaaga tcacgtacta cagcgggtac tgcggcccgc
     5221 tggagaggat gttcatgccc caggggaacg tggttccctt ccccatgcac ctttccaagg
     5281 catacaaggc gctggaccgt gccaaggcgc acgatgtggc catgatcgag gtgaccccgc
     5341 ccgacgaccg gggctacatg aatttcggcc cctgcggcgt ggcctatggc aggtatgcca
     5401 cgaaaaagtg caggaaggtc atcgcccagg tgaacagcaa gaccccgtgg gtccatggca
     5461 tcgagaacgt ggttcacgtg agcgaggtgg acttcatcgt ggaaaaggac cacgatctcc
     5521 ccgtcgtgcc cgagatcgag ataacggacg tggagaagaa gatcggcgag cacatagcag
     5581 agcgcatcga ggacggcgcc accatccagc tgggcatcgg gggcatcccg aacgccgtcg
     5641 cctacttcct ccacgagaag aaggacctgg gggtccacgc ggagatcctc tccgacgcca
     5701 tcgccgagct cgcggagcta ggcgtcatca cgggcaggaa gaagaccatc ttccgcgaca
     5761 agatcgcggt gggcggcctg atcatcggct ccaagaggct gctcgacttc tgcgacgaca
     5821 acccgaccat cctgtccatg cccatccaga tcgcgaacca ctacgacacc atcaggcgcc
     5881 acgacaacct gtgctccatc aactcggcgc tcaccgtgga cctcacgggg cagatcgcgt
     5941 ccgagaccat cggccactta cagtacagcg caacgggcgg ccaggcggac ttcgtgcgcg
     6001 gcgcgctcgc ctcgaagggc gggaagtcgt tcatcgccct gccggcaacg ggcaggaaca
     6061 aggacgggtc cctcatgtcg cggatcgtgc tgaacttcgc gccgggcacc gtggtgacca
     6121 cgccccggtc cgacgccatg tacatcgtta ccgagtacgg cgtggcggac ctgtacatga
     6181 ggtccatccc ggacagggtc aaggccatga tctctatcgc ccacccggac ttccgggagg
     6241 acctggagag gcaggccttc gaggcggggc tcctgatccc cgcgacactg ccggtgaacg
     6301 tctgagggcg cggggtgtgt aaaagtagcg tgaacgatcg tttcacaaaa gacgtttcct
     6361 tttgcggcac gatcgtgtat gaaaggaagg ttccgtgtct ccctgtgccc gcagggggtc
     6421 acgggtgcct ttggaattcg atgattccgt caagaaccga gtaaaggaga gtgatatgaa
     6481 ttatctgatc cagccaaaaa agttcaagag tctcatggcc gacgagggct cgcgggccat
     6541 catggagaag accatcgcct tcttcgagaa catggggaaa cagaggcttc tcgacgacta
     6601 ccacagggcc atctggtacc gcgagttcat cgacttcatc ggcagggaac ggatcttctt
     6661 caagctgctc acgcccagcc agtatgcgga cggagacccg gacgcccgct gggacacggc
     6721 gcgcaattcc gagtactcgg agctgcttgc cttctacggg ctcgggtact ggtactgctt
     6781 ccaggtgacc atcctcgggc tcgggccgat ctggatgagc cccaacgaga aggcgaagag
     6841 gaaggccgcg aagctcctca gggagggcgc catcttcgcc ttcggcctgt cggagagggc
     6901 ccacggagcg gacatctatt ccaccgagac cgcgctcacc ccgaagggcg acggcacgta
     6961 cgtggccaac ggcgagaagt actacatcgg caacggcaac gaggcggaga tggtctcgac
     7021 gctcggcaag gtgaaggacg ggacggacga ctacgtgttc ttcgtcacca actaccggca
     7081 caaggcatac cagctcaaga agaacgtcat aaaccaccag gagtacgtct ccaacttcgc
     7141 cctgaacgac taccccgtca cggaggacga catcctctcc agggggccgc acgcgtggga
     7201 ctgcgcgctc aacacggtca acatcggcaa gttcaacatc ggcccggctt ccatcgggct
     7261 ggccgagcac tgcttctacg aggccatcac gcacgcgtcc aacaggatac tctacggcat
     7321 gagggtcacc gacatgcccc acgtccggaa gaacttcatg gacgcctggc tcaggctcgt
     7381 ggccatgaag atgtaccagc gccgcgccac cgactatttc cgcaacgcat cccccaagga
     7441 caggcgctac ctcctctaca accccacgag caagatgaag gtcacgctcc agtccgagga
     7501 cgtggtgaac ctcctgtggg acgtgatcgc ggccaagggg ttcgagaagg acacgtactt
     7561 ccacatggcg gcctcggaca tgcgcggccc gtccaagctc gagggcacgg tgcacgtcaa
     7621 cgtccagctg atacggaagt tcatgaagaa ctatttcttc aacccggccg agtacgcacc
     7681 cgtgccgccc gacttctccc tcaaggacga catgttcctc ttcgaccagg gtccgaccaa
     7741 gggcctgggg tccatacagt tccacgacta caagcccgtc ttcgatgcgt tcaagtcgct
     7801 gccgaacgtg ggagcgttca tgaagcagac cgagatattc aaggagatgc tcgagaaggc
     7861 gggtccggac aaggcgcagg acatggaccc gtcgtggtcc ttgccgctgg gcgagatgtt
     7921 ctccatcgtg gtctacggcc agctcatcct cgagcaggcg cagatagaca aggtcgaaga
     7981 cgacgtcgtc aaccagatct tcgactttat ggtgcgtgac ttcgcccact tcgccctcca
     8041 gatctacggc aaccacgcct ccacggacga gcagcgcggg tactgcacga agatcatgct
     8101 catcaaggcg gtgcctgcac ccgagcagta ccagagggtg tgggagaagt acgtgtcggc
     8161 cctcaacggc gagtacgaga tgaacccgta acggactgag ggccggaggg ggctgcatcc
     8221 ccctccggcc caatccgtac ctgcatccgt cggctgcccg gggcacgggc ctgctccggg
     8281 gggcccgggt cacgacgtcg tcctgcgggc atgtgacgcg ggcagcaagg ggcgaggccg
     8341 ggtcgatgaa gaccttcccg cttccccgcc gtgacctgtc cggaaccctt tggccgggaa
     8401 tggaggcgag cctctgcggt ccgtccatgg tgatcgcggg taccgaaaat gcccgtttca
     8461 ctccgggtgg gccgtggatg cctcggcggc cggcttcacg gggcgttccc gacccctcga
     8521 tgaccccccc tgaaaatggt ctcaagtaaa gagtcgtgat gccgataaga taagcgaaga
     8581 actctcgaaa ggggaccgcg atgaaggtca gtcctgaaat gggtaatgta atcaacataa
     8641 taagaagaga gggtggggat caggccctca ggaaggccga tgcggccaag gagagaaagg
     8701 agatcgagga cgtcgtctcc atcgagaaca ggcaggcatc gagccccgag gtgaagagcg
     8761 tggacgaggc gagggatctc ctggcgtccg tggtcgaggc cctcaagcac gcacccacgg
     8821 atctctatgc cctcgacgtg aaccgcgtct ccgtcatgat cagctgaagc aggcccttcc
     8881 cacagaagag acagaaccac aggccgcctc cgtgcggtct gtggtttttt ccgtgtggtc
     8941 gcccccgctc gtccctgtct ccatacccgt cacacattct cgaagacccc gtgtttcctt
     9001 gccgatccgg tgggcaagaa aggtctctgc gacatgccgt tgccgcaagg ccccgggcgc
     9061 ctggtgaccc tccggcggtt cggatgaatt ttgtcctttg ccgccaggcc ttcggcacaa
     9121 ggcgcgcgcg ggtatgagtc cagggccggg gccatgtcga tatggccctg attcggcaca
     9181 aggctcgtgc gggtatgaga ggggggaggc gatcctgggt gccgtcagtc cttcgcaggg
     9241 gtggactcgg cggaacgggt cgtctgtgag gtctcgggct cttccccgtg ttccagcgtg
     9301 tcgtcgagca tgaccctgta gatgcgcttt atgtcacgcg tgatctcggg cgtcaggatg
     9361 tcctcgaccc tgggcggcct gttgacgaag cggtagtagt cgtcgccgat gtacggcccc
     9421 gagatctcct gtgtgaaggc gcgcgccgcc acgtcggaga tggcgaggat ggagagttct
     9481 ttcacgatgt ccccgtgcgc cttggcaagc tcgcggacag gccagaacat cggccagtga
     9541 tgggccgcga tgcaggcgac aagcctctga ggcatgcccc agtgctttgc gaggagccct
     9601 ccgagcgtcg cgtggtcgat gccgtagtgc tccgtctcct gcctgagccg ctccctgggg
     9661 tctttcgaga agccgccggg gggtgcgggc aaggacgcgg ccatgacggt gagcgcgata
     9721 tcgtggagca tgccgagggt caccatggtc cctccaggca cgccgatgcg gctcacaccc
     9781 gtgaggtagg ccgctatggt ggaggtgacg ttcgcgtgga cccagtcggc cttgtcctgg
     9841 cggatgccga tcttccgtgt gcgctcctcc atgacgatcc ggtagatctc gttgtagccg
     9901 aggatgacga tggcgttgaa gatgttgtcc accttctgca gcaggccgta gtacggcgag
     9961 ttcaccctcc tcaggatgaa ggaggtcagg ccctggtcct ggacgatgat cttgctgatc
    10021 tccctcgggt tgatgtcagg gtcgttgagc ttcgaggaga gctcggtgat gagcggggag
    10081 atctgctcga tcttctccac gagttcgagc acggactcgg gcgcatggga gagggcgtcc
    10141 ctgtcgggtt tccattcctg gttgtagacc tcaaggaaag aaccgatgtc ctcctgcggc
    10201 gagacgctga cgtatctggc gggggccttc cttgtcggct tggccggggg ttgcgaacgc
    10261 ttggtgtcgg ccacgatcca gaccagcacg ccgacggcaa ggaccacgac gacaagaatg
    10321 aaggcgctca tcaccatcat ggtctcttat cgggaatcga taggctattc tttaagtgca
    10381 tgaccgttct gtcatcggca gacgtcgagc accccctcgt cctgaagaaa agcagcccgg
    10441 actcgatcgt gagccccgct cgcgcctcgc atccacggtc tttcgtgggg aggacccccg
    10501 ccttccggtc gagacgtgac cgggcgtaac ccgtggttca aaagtgcacg aggggcctcg
    10561 gagagagggt cgtccgggca ggcaaagttc gttcacgggg gccgtccttt cccatgcggg
    10621 tatgcgtgaa aggcctttgc cccctggtga atgtctttgc ccaggcgcac ccggtgccgt
    10681 gacagccgcg ggtgttcaag tgggccggtc ccctttccga aggaacaggt atggcagaca
    10741 ggcggacacc gagcctcagt gtgggatgcg tcccgccggg gaaagggcgc acatgtgtgg
    10801 cggcccggca ggctggccgc gtcgcatgcc gtgaagaggt gacacccgag agaaggcgcg
    10861 atcatggtag agcacgttga gacgccatcc cagcgggaga aaatcctcgc gatcctcgag
    10921 agggcgcccg atatcctgac ccttcccggc atcatccagc agatcctgga ggtgacgtcc
    10981 aggaagaact cctccgcttc ggatctcacc gcgatcatcg agagcgaccc ggcgctcacg
    11041 agcagggtga tcgccgtggc caactcgccg tattacgggt tcgtcaagaa gatcacgaac
    11101 gtgtcgcatg cgatcgtggt gctgggtttc agggagatcc agaacatcgc gctcagcatg
    11161 tcggtgctca agatgttcga ccccaagggc tcagagttcg tggaccagct gtggaagcac
    11221 tcgttctgcg tcggtgtggc ctcgaggatg atagccgagc acctgaggct gaaggtggag
    11281 ggaaagtact tcgtgggagg gctcctccac gacgtaggga agatcttctt ctcccagtac
    11341 gtgttcacga gcttcgacag gctcctgagc gtcctgtggg aggaggacac catggagagc
    11401 taccacgcgc tggaggagag gatgctcggg atcacccacc aggaggtggc gaggatcctc
    11461 ctcggcgaat ggatgttccc tgccgagatc gtcgacgccg tggcctgcca ccacgacccc
    11521 gcttcctcgg aggtcgaccg cctcttcgcg tgctgcatcc acctggcgga cgtcatctgc
    11581 accgccaagg gcatcacccc gctgaaagac cgtcacttcg tgctcatcga ccggggggtg
    11641 ctccggttcc tcgaagaatg tgggcacacg ctcaccacct ccgacgtcaa gtccttcatg
    11701 gacaggctcg acctcgagat cgagcgacag agcacgtacc tgtccgcctt caggaaccgg
    11761 taaagaaggg cgtgcggctc tgggtgcgaa cctgcttcac ctcgccacgg tatgggttcc
    11821 ggcatggggc gagtgcggtc cgtgcggaga ctcgacggga ccgacatgaa cgtgcgaggt
    11881 ctgcccttgg ccccggtgaa ggcgtttcgg cccctggcgg tgactccacg gtatcacggt
    11941 cctcatgggc cgagaagggc ccgcaaccgt aagagggcat gggaccccga gggtgaagtg
    12001 gtcacgtcgg gcctgtaggc gggccgcacg tggcaggccc cgggcgctct tcacaggccg
    12061 gctcacgcca tcgaccggca gggctcagcc gatgagcctg gccagcatgc tcgatccctc
    12121 ctcgacgaac acgccggtcg ctctcgcgtg ggcctccgtg aacctcctgc cgggggtctt
    12181 gcctggcgca ccgctgtcgt agaggatata gggcacagga tcggctgtgt gcgtcttttt
    12241 gcagatgggg gtggggtggt cgggcatgac gagcagcctg aacggtgagc ccaggcggcc
    12301 caggccttcc aagaccgggc ccacgatccg tgcgtcgaag tcctcgatgg cctggatctt
    12361 cttttctacg ctgccgctgt ggccggcctc gtccggggcc tccacgtgca cgtagacgag
    12421 atcgtggcgc ccaagcgcct ccagggcgga ggaggccttc ccctcgtagt tcgtgtcgag
    12481 ccaccccgtg gcgccaggta cctctatgac ctccatgccc ctgagctttc cgaggccctt
    12541 gaggaggtcc acggccgaga tcatggcacc cgtgatgccg tactcctgct caagcgtcgg
    12601 cacccgcatg gccttgccct cgccccaggg ccagagggcc gtggcgggcc tgagccccct
    12661 ggacagcctg ctcctgttga cggcatggtt ctcgagcagg gccatggaac gcctgatgat
    12721 ctccatgagc agctcgtcgt tcgggaggta cgcatcgacg ggctcgccgg tgatgtcgtg
    12781 cggtggggtg gtcttgaggc cggggttgtc gtagcccctg aggaccagga ggtggcggta
    12841 ctcgatcccc gggtgcatgc ttgcgcccgg catgtcctgc acgatggact tgaggtcctc
    12901 gatgagcgca cctgcctcct gcgtggatat gtggtccgag cagtagtctt ccatgaacag
    12961 ggtgccgtcg cgccgggaga tggtgacgag attgatcctg aaggccgtat ccccctcacc
    13021 cagcacgacc cccatgctca cggcctcgat gggagccctg ccggtgtagt acctggacgg
    13081 gtccagcccc atgaccgcca tgttcgccac gtcgctgccc ggcgggaacc cctctttcac
    13141 ggttctcacc atgccgagtt cgcccgagac ggagagcctg tccatgttcg ggatgcgcgc
    13201 gcactccaag ggcgtcttcg agccgagttc tggaagcggc tcgtcggcca tgccgtcacc
    13261 gagcaggatg atcctcttca ccttcgttgt cctccgacgt tcaaaaaagg cctaggacgg
    13321 agagttcttg agcttcttgt cctcgacgcg gatcaccacc ggcttcccgt tcacctcgga
    13381 caggctcgat atggcctcca cggcgctctt cacgccggcc tcccgcgcct cgtgggtgag
    13441 catgaccacg gggacgaaat ccacgctgtc cgtccccttc tggatgaccg agacgatcga
    13501 gatgccgcgc tcgcccagga tccctgccac cttggccagc acgcccggcc tgtcgagggc
    13561 gttgatcctc aggtagtagt tcgtcaccac gtcgtcgatc ggcaggtacc tcttgggcga
    13621 cgagctgccc ttgcggtagc ccaaatgcgg tatcctgcct gcggtgcccg acaggatgtt
    13681 cctccctatg tcggccaggt cgctcacgac cgcggatgcg gtgggcatca tgcccgcacc
    13741 cctgccgtag aagagggtgg agtccacgat gtccccccgc acgtagaagg cgttgaacac
    13801 gccgtccacc ttggagagca ggtagttggt ggggaccagg gtggggtgga ccctcgcctc
    13861 cacgctgtcg cccctgtcga tggccagcgc aagcagcttc accttgtagc cgaggtcgcg
    13921 cgcgaacgtg acgtccatct tgtcgagccg ggtgatcccc tccacgtaga ccccgttcat
    13981 gtccgggggt acgccgtagg cgaggctggt cacgagcgcg agcttgtgcg ccgcgtcgat
    14041 cccctcgatg tcgagggtgg ggtcgctctc cgcgtaaccg agcttctgcg cgctcgagag
    14101 cgcctcgtcg aagctcgagc ccttctcgcc catctcggtg aggatgtagt tgcacgtgcc
    14161 gttgaggatc ccgtagatga acgcgtagtt gttcgccacg agggcttccc tcaacgtctt
    14221 gatgatgggt atgccgcccc cgacggcggc ctcgtagtag aggtccctgc cgaaggtctg
    14281 cgccgcttcg aagatctcct tgccgtgggt ggccaacagg gccttgttgg ctgtgaccac
    14341 gtgcttgccg cgttcgaggg ccttcaggat gaacgtcttg gccggctcga tcccgcccac
    14401 gagctcgatg atgatgtcga tgtcgggatc ttcgaccagc cgcatcgcgt ccgtggtgag
    14461 gaggccctgg ggaatggcta tgccgcgatc ccgcgtgatg tcgatgtcgg cgatcccctt
    14521 cagggacagg gaaaacccga gcctgtcctc gaggagtccg cggttttcca tgagggtcct
    14581 ggcgaccccg gtcccaatgg tgccgaggcc gatgaggcct atcctgatcg tctccatacc
    14641 ttctccttcg tcggtgaacg gtcccctgag ccgcgcgcgg tcctgatgtc gtcgagggtc
    14701 tcccgagccc tgaaagaact cctcacgagc ggcccggcta cacaatatcc gatgccgagc
    14761 gatttcgcaa ggccggccca agtctcgaag gcctcaggtt cccagtatcg acggacctct
    14821 acctggcgca tcgagggcct caggtactgg cctatggtca cgatgtcgca accggcgccc
    14881 gcgagcgagc gaaggaggtc ctcgatctcg tctgcgcgct cgccgagacc gaccatgaat
    14941 cccgacttga cgaccgtgtg aggggccagc tcccgggccg cctcgaggag gccgaggctc
    15001 cggcccaggt cagccccggg gcggaccttc tcctgcagcc tcccgacggt ctccacgttg
    15061 tgtccgaaca cgtcgggccg ctcgctcaag acgagtgaga gcgggtccct gaggcctccg
    15121 aaatccgggg tcagcacctc cacggtacag gcggggatcg agtctttgat cgctcgtatg
    15181 gtccgggcga aggcgcgggc gcccttgtcc tcgaggtcgt cccgggtgac cgaggtgatc
    15241 acgacgtgct cgaggcccag catgcgcgcc gcacgcgcca cccgggcagg ctcagtgggg
    15301 tcgaccggcg ccgggacgcc cttcgccacc ccgcagaaac ggcacgccct cgtgcacaca
    15361 tcgccgagga tgaggaaggt ggccgtcttg ttcgcgaaac agagggaggc gttcggacag
    15421 cgggcctcct cgcacacggt ggagaggccg caccccctga ggagggcctt catctcgtgc
    15481 aggcacccgt gatcccggcg gacgtgcacc cacgcgggca gcctggaaga cgagccccgg
    15541 ccttccacgg tcttttcttg cgtgtcctct cccgggaccg gggcgcggga tgcgcaagac
    15601 ggcatcacat ccggcgttcc atgctctccc atctcggtct tctacctctg gcccccggat
    15661 ccgccggact tcttgagcct ctccctgagc tcgtccctgc gcctcgagag cgccttgatc
    15721 tccttgccgt cgttcgatgc ggcaagggcg gacaggatgg agaccgccgt gcccgcggcg
    15781 aggcagccga acagcacaag gtagaggggg taggtcaggg tcttgaacga gtagaggtgg
    15841 acggtggcgg gttcctggtt ggaggccgcg aagaggacgc cgatgacggc gagcgccacg
    15901 aaggccaggg ccttgatgac cctcaagaca gggcctccct gatcttcttg tgcgtctgct
    15961 cgatgtgctc ggagatcacg cgggtgtgtc ccacggccgg catgaaattg gtgtcccctt
    16021 cccaccgggg caccacgtgg aggtggaggt ggccggcgat gcccgcgccc gcgacctcgc
    16081 cgatgttgat gccgaggttg aacccgtccg gcttgagcgc gcatctcgtg gcctcgacga
    16141 tcttctggga aaccgccatg agctcggccg actcctcggg ggtgagcgag cagatgtccg
    16201 cgacgtgtcg gaagggtacc accatggtgt gccccgtcgt gtaggggtag cggttcatga
    16261 tcgcataaca ggtggcgccc gaggtgagga tgaggccgtc ttccctcctc ggcgcctcgc
    16321 acaggacgca cccgccgtcc ttgtccgaga ggatgtattc cattctccac ggcgcccaca
    16381 ggtttttcat agctcgatga tcgtcccttc cacgcgctct gagtccaccg tgattatacc
    16441 caccgagcgc atggttgtaa atcgattgtc ggttggggac cccgggttga agaagtaggt
    16501 gccgtcttga gttccggcat acgcctggtg ggtgtgcccg tagacgatca ccccggcatc
    16561 gggcagcgcc cggcgcaggc gtgacctgat cccctcgggc gatccccagc cgtggatcag
    16621 cccgatggtg accccttcca cggccagggt cctggacatg ggcaacttcg cccgcacgtc
    16681 ctggccgtcc atgttcccgc acacggcgac cacggtgtgc cccgcctcct cgaagacgcc
    16741 gagcacgtcc atgcacacga tgtcacccgc gtggagaacc aggtcgacgt ccttgaagtg
    16801 catgctcgcg atctcttcga ggatcctctc cctggccctc aagtgggtgt cggagaccac
    16861 gccgatacgt gtcatgtcct ccccctcctc catggtatgg tcgacagtgt ccagcctgcc
    16921 cgtcgttcag ggctcgcacg ggcctagagg tacgcgtcag cgtcgggata gatgctcttg
    16981 acgtcgtaca cgacgcagcc gggcgccttc aggcgcgaca ggtccatgtc cctgaacgcg
    17041 tcgtgcgcca ccgcggcgac gatgccctcg taggtgccct gtatctcgtc ttccgatgcc
    17101 atgatctcga tgccgtatgc atccctcacg tcttgggggt ctgcgaccgg gtcgaagaca
    17161 tccaccgtgc actcgaactc cctcagctcg gtgatgatgt ccatgacctt ggagttcctg
    17221 atgtcagggc agttctcctt gaaggtgacg ccgaggacga gcacgcggct gtccgttatc
    17281 ctgtgcttcc tcttgaccat catcttgatg agctcggtgg ccacgtacct gcccatgctg
    17341 tcattgatcc tgcggccggc gaggatgacc tgggggatgt agccgagccc ctcggccttg
    17401 tgcgtgaggt agtacgggtc gacgccgatg cagtgccccc ccacgagccc gggggaaaag
    17461 ctcaggaagt tccacttggt cgatgcggcc gcgagcacgt ccgaggtctt gatgcccatg
    17521 cgggcgaaga tgagggcgag ctcgttcacg aaggcgatgt tgatgtcgcg ctgggcgttc
    17581 tcgatgacct tggccgcctc cgccaccttg atggaagggg cggggtgggt gcccgccctg
    17641 atgatggagc ggtagagttc gtccacgatc cgggccgtcc gaggggtgga gccggaggtc
    17701 accttgacga tatctgtgag gcggtggcgc cggtcgccgg ggttgatgcg ctccgggctg
    17761 tacccgcaga agaagtcctc gttgtacgtc aggcccgagg ccctctcgag ggcggggacg
    17821 cactcctcct ccgtgcagcc ggggtacacc gtggactcga agatcacgat gtcgcccttg
    17881 ccgagggccc tcccgacggt gttgcacgcg tccatgagga ggctgaggtc gggcctgttg
    17941 taggcgtcga tcggggtggg gacggtcacg acgtacgtgt tgcacctctc aaggtctgat
    18001 gcatcaaagg tgtagtcgag caggcgcgcc tcagcaagct ccgcggggct cacctcgcgg
    18061 gtcctgtcca tacccgagcg cagttcctct atcctccgcg ggttgatgtc gaaccccagc
    18121 gtgggatagc gcctgccgaa ctcgacggcc agggggagcc cgacgtagcc gagcccgatc
    18181 actgcgatgc ggtggtccat cgcttctcct tccgttcgct gatgggattg tcgtggtttg
    18241 gagagggtat atacagcaca acccgaaaac atgaccaccc caaaaatgtc ggggcaccgg
    18301 cggggtccga ggccgtcggg tgtcccaaac ctcgaagcgc ctgtacgcgc ctgcgcgtcc
    18361 cccaggatgg gcgctcgagg aggagtgcac gaccgtcagc ccgggcagac gacgggttcg
    18421 ttctcggccc gccttgacag ggggagggaa accttctaga gagataccgg ctcctgctcc
    18481 acactacccg cgaggaagct ggctccccat gaacggacac atttcgcgca tcggcgtatt
    18541 cgactcgggc atcggcgggt tgaccgtgct caagtgcctc ctcgatctcg tcccggccag
    18601 ggagatgatc tacctcggcg acacggcccg gctgccgtac ggcacgaagt ccccccggac
    18661 gatcgtgcga tacagcctcc agtgcggcag gttcctggcc gagaaggggg tggacatgct
    18721 cgtcgtggcg tgcaacacgg ccagcgccca cgcagtcccg gccctgaggg aggagcttca
    18781 catccccgtc gtgggtgtgg tggaggctgg ttccagggcc gcggtggaga caggcggcag
    18841 gaagatcggg gtcatcggca cctcgt
//
LOCUS       DAOSCR010000003        15257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_2665_length_15257_cov_3.514669, whole genome shotgun sequence.
ACCESSION   DAOSCR010000003 DAOSCR010000000
VERSION     DAOSCR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 15257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15257
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2665_length_15257_cov_3.514669"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..321
                     /gene="rpmH"
                     /locus_tag="PLT69_00195"
     CDS             187..321
                     /gene="rpmH"
                     /locus_tag="PLT69_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOJ12900.1"
                     /translation="MKRTFQPHNTRRKRTHGFLVRMRTKGGRLVINRRRAKGRKRLSV
                     "
     gene            321..689
                     /gene="rnpA"
                     /locus_tag="PLT69_00200"
     CDS             321..689
                     /gene="rnpA"
                     /locus_tag="PLT69_00200"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOJ12901.1"
                     /translation="MPDERFPSRERLRKRAEFKRVEQYKTARINTRHLVIVAAPNDLE
                     HTRIGITASRKIGGAVSRNRVKRLLREVYRRNKPSFPRGHDVVLIAKNDMSDITYHDL
                     AGEVTAALGSLPWERKPSSH"
     gene            662..901
                     /gene="yidD"
                     /locus_tag="PLT69_00205"
     CDS             662..901
                     /gene="yidD"
                     /locus_tag="PLT69_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOJ12902.1"
                     /translation="MGKKALIALIRIYQAIVSPMLGPACRFHPSCSAYAIEAIDTHGP
                     LRGTILAAWRILRCNPFCKGGFDPVPGTKTPGECT"
     gene            898..2529
                     /gene="yidC"
                     /locus_tag="PLT69_00210"
     CDS             898..2529
                     /gene="yidC"
                     /locus_tag="PLT69_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOJ12903.1"
                     /translation="MMDENQRTMLALAISALILVAWFALFDSLNIGRKEAPETANQEK
                     KATSAPQTLQGKPGQAPGNLPAGPAPVQAAAPQQETERTIVVDTDLYRATFSTRGARL
                     TSFKLKKYPITMEKGSDPVEMIKAPVPGLDIEGIGSDAGLVFTTPARDRVTLNGSGGS
                     IEFRREVAAGVTLVKTFTFQPGSYLVGFRVGLSNATGSPVRLSGRLVFDSVYPLSEKI
                     KGYAFQGPVLLNDNRLEEFKIDKVKKPGAYREFTGSIGWFGVEDKYFLHTLVIPRPYQ
                     TTVTVRRLDDRVVELSCALPTLTVKQATAVAIDHSLFIGPKELNTLKAAGNELARALD
                     FGFFDFIAKPLLVAMNWINRYTLSYGISIIVLTVIIKILLYPLTLKSFKSMKELQKIQ
                     PLMKEIQNQYKDDKQKMNQELMRLYQEHKINPMGGCLPMLLQIPILFALYRVFYQAIE
                     LRHTPFHIVGTWLPDLSEKDPYYITPILMGLTQFVLQKMTPATGDPTQQKVMLMMPIV
                     FTFLFLNFPSGLVIYWLVSNLLSIVQQAYINRKHA"
     gene            2540..3286
                     /gene="jag"
                     /locus_tag="PLT69_00215"
     CDS             2540..3286
                     /gene="jag"
                     /locus_tag="PLT69_00215"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOJ12904.1"
                     /translation="MEDHAQEFEAKTVDEAIILAMKTLHADFEDLDITVVSEGSKGLF
                     GIVGSKNAKILARKKGPGSRPGTDRVVPPPEAPAPRQPVDTGRPGSPDTQAAPAQDVL
                     EQAKEVVSRILGLIGLRAEVRPRDDGMLEIVGDGSGIIIGKHGQTLDALQFIVNRIVN
                     KSRQDQVHVTIDTEGYRKRHIDHLKALAIKMGQKAKKTGHPVTLEKMNPYERRIIHLA
                     LKDERFLDTKSIGEGVYKRVMIVPKRASRR"
     gene            3301..4038
                     /locus_tag="PLT69_00220"
     CDS             3301..4038
                     /locus_tag="PLT69_00220"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12905.1"
                     /translation="MYAESTPHGTGGISLVRISGPNTSAVLSRICSRRSFAPHVQHLT
                     VLRDEAGATVDQVTVVFNPQPRSYTGEDLAEIGCHANPIVVERILGVIASTGLARRAA
                     PGEFTMRAFLNGKMDLVQAEAVRAFIESTTDAGCDMARRLLEGGLSSRIKKLRASISE
                     ILADIEASFISEDEVVAAEELAARIEPLAAELAGMSGDACRGLKVYGGVTTTIAGLPM
                     WGSRPSSTPSWAATGPSSTTRRGRPGT"
     gene            3954..4601
                     /locus_tag="PLT69_00225"
     CDS             3954..4601
                     /locus_tag="PLT69_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ12906.1"
                     /translation="MGKSSLFNAIVGSDRAIVHHEEGTTRDVIRERVTIDGMDFLFHD
                     TAGIRTTPSGPEQAGVERAIAAVKASDLLLYVVDARVGLQPHESQWLGLGGTTILVVN
                     KIDLVKEPAITETGYRTVMVSAKHSTGIDALKSAMVETFRPANPEVFLDRHAHLLSEA
                     SRHLDSCLSALSQGMTLDVASIELSGALRALGAITGESADMDILEQVFSRFCIGK"
     gene            4603..5145
                     /locus_tag="PLT69_00230"
     CDS             4603..5145
                     /locus_tag="PLT69_00230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ12907.1"
                     /translation="MDDRLAKWMGELRRFNPRLHLVGPSMLEHLEEEVEVTSRLLEGI
                     TEPAIADLGSGSGLMAFVYKALHPDSIVTCVERSVKKCDFLRHTAEVLGLEGFCVLCE
                     DLLASGRRFKAAMARSFSPVATLGKICSRVLVEGGRLYYLSSSNPQEMGSGFRLDGIQ
                     TARSGTGLTLNLWRFTRLRP"
     gene            complement(5126..5569)
                     /locus_tag="PLT69_00235"
     CDS             complement(5126..5569)
                     /locus_tag="PLT69_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ12908.1"
                     /translation="MASSHSLRGDLLFLRERMSRAFEESMQDSVMVAGPGRFVPCVDI
                     YEDDSTLYVKIELPGVRREDIALDITDGVLTISGTKPCDHVDAAENFHLVERQYGLFR
                     RSFSLPESIDLDRVEASFNAGVLEISLPRLETRVSRKIPVTGEAG"
     gene            complement(5583..6440)
                     /locus_tag="PLT69_00240"
     CDS             complement(5583..6440)
                     /locus_tag="PLT69_00240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOJ12909.1"
                     /translation="MHLPVPYHHEVAALVAKFDQIPALTKEEEFELAVRLREQGDVDA
                     AHRLITANLRNVVRIAMDYAGYGLPLEDIIQEGTIGLMIAVKKYDPHKGYRLMTYAVW
                     WIKAMIHDYILRFFSQVKLGTTKLQKKLFYGLNRMSSEEEVNHEPIGEQSRALSARLD
                     ADPRRIEEIISRLTGRDQSLDTPMAEDSDSSFLDFLADTRPGPEDRVIESQRSAFVKR
                     QIESALAKLTEREREIARHRLMAEQPMTLEDLGRRFSISKERVRQIENAAKGKLKKAL
                     AGNLELMLQ"
     gene            6588..8126
                     /locus_tag="PLT69_00245"
     CDS             6588..8126
                     /locus_tag="PLT69_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ12910.1"
                     /translation="MMSFRAVIISGVVFAVMGCSSGLHYTYPPPHHPSPVLDLAKVQD
                     MQETKPRDHHQPPHQSPAIPTTEETKAIPRGNLASLGVAPDQSVSRHPASSSPEDEHD
                     RAPAGRGEIRDTTTAGEALPDGTWLPAGGLRPRVNAWIERLTGKEKDTFSSQLARLER
                     IRPTIERIFSEQGVPAELSYLCLVESGARPDAMSPSGAVGYWQFMPDTARRFGLQVNR
                     YVDERKNLEKSTLAAASYLKHLYAMFGDWYLAIAAYNAGEGTVMRLMQAHRLRSYWDI
                     DAGMAIKYETIDFVPKYIATVIIAHTYERYGLKPPSSPLSRGLGIASGGYLPGSEPYG
                     QSSRSRMALAPPLAVTDAPLGTRTSSERDAPQAGDTTADAGGRDCLRPLTHTVKKGDT
                     LYSLAKRYDTTVKSLCEANHLKSSRHIHPGMRLEIPAAGKTPPRGTPDREPRVSGAAQ
                     MKAKGGPAREGKNTPTYTVKKGDTLWSVARRYEVRPADLAAWNDIGPDGVIKPGERLV
                     IRPR"
     gene            8775..10070
                     /locus_tag="PLT69_00250"
     CDS             8775..10070
                     /locus_tag="PLT69_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ12911.1"
                     /translation="MKKLSFALACMCTLALTIGAPRHLQGEATWSQASYVTDEDLFCV
                     TGWSDGEIYACGEDGELVGFDGVSWDEYPVPRAFDLRAIALLATGDLLVTGDMGIVLT
                     GSPGGVLAEAYPVLTDLNALWLSSDDAVITCGDLGKVLVSDGDSWSDMSPENAQAFDL
                     YCAWGTGEEDFWVGGSFGRLWHYRSGRWASYPMTDLTTQTFRALWGASEDAVFAAGTL
                     GHILAYDGSSWSIVHTTDGLAVYALWGTSDDDIFASGEMGFVYHYDGGTWSRIRSPGT
                     LDIYAIWGGTSGRVYFVGENGLFLVYSREDTYAPSVVHTSPTGGSTGVPRDTSVVFVL
                     SEEVDPSTVDNASVRLTDGNQEVQARVSSSANRVTLTPEEALEPSTRYTATLSQQVAD
                     PSGNRLGQAYTISFTTAATTGEDDGGGGSGCFVACCSAY"
     gene            complement(10404..12062)
                     /locus_tag="PLT69_00255"
     CDS             complement(10404..12062)
                     /locus_tag="PLT69_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ12912.1"
                     /translation="MRWGIAAFLAAVLLSPVSPHAQDITLVKHARRIAPPPGDTYVVK
                     QGDTLRRILMEVYRARENDLPDLYARFRQLNPDIADLDSLMVGKSIKIPVNSVAGRER
                     APAGTPSGRLGVEEVSAEEYVVRQGEYLSKILKEIYGVPDDLVFSRYIEMVKRLNPQI
                     TDPNRILAGQKIRIPSVRQILADSKQGQGKNGRKAGPAGDEALAVDRENGGQDAGPRA
                     GADQATGATNETGRVARAKDVKGKLLPALEAMGGSSQGTGTYYVPVTGGAGMAIDTRE
                     IPVMELDTGKKIILDLEGKITPEVRSYIEKAFPSISVVSGPEADMESLIDRVLSVSGF
                     FSVNKDPAPLMVGSEEKVSLFGKWLVYKDFSRRNVYVINLLGERDSKIPQPIRRYVQA
                     FGIDIVEIGGVEPRVRTDRPMAPVSVSGSYRELFKALSIPFERDKEIELVSGGPVRIV
                     YKAPVVSGRVIVADGSPDAQTASLLSKRSFQVFDGSRASPQEVLAGLGFTLEGPPVRL
                     KVAQGKAELEIPAVKTGATMILLSRIDQVIQQYIASLGYRLIVW"
     gene            complement(12135..14459)
                     /locus_tag="PLT69_00260"
     CDS             complement(12135..14459)
                     /locus_tag="PLT69_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOJ12913.1"
                     /translation="MRWLKFVLLALLITVCFVSLWTYLIIFRDLPSIEDLKAPESTRV
                     SKVTAEDGTVLGYFIPEGRVVMNGHGIPLTLKQAIIAAEDSTFYDHIGLEPRRILSAL
                     VADIRANSFVQGASTITQQVVRSYLLTREKTLTRKIKEIVLALRIERALTKPQILNLY
                     LDRVYLGSGAYGVQAASLRYFGKECRELELSEMAMIAGLAPAPARYSPLNDFTACKRR
                     QWYVLNRMVEEGYITEEEARAAYGEPLRIVAKESALFTEEPYVTEYARTLVVQQLGEE
                     IFDRGIVVRTSIVPSLQRAAYRAVRKGVMELEMRQGKYAGPVRGVIQPDGSMKPFTIE
                     DKERLFSFQTNQIEWKGYEPYELYWAEVVSVEPLVVKLGTRRVELGPASYEWINPKGR
                     WSPAKHLRPGDLIMVCYTPQGFVISQKPTVQGVLLAFDLNTGGILAMVGGVDYSQSQF
                     NRAVFAKRQSGSAIKPFIYAAAIDKGMTPATIVFDAPVSYNAGADEEPWQPKNFENKF
                     FGATSLRTGLVLSRNVVTIKILGEIGIDYAREYIDRFDMKTKLPRNLSIALGSADVTP
                     YNLFKGYAVFALYGRRFEPHLIESVVQSGKGTILQAPPPIRQVAQTGEPGGPVYEQVI
                     SPQTAYIVTDILKDVVREGTGWRAKALGRPVAGKTGTSDDSRDTWFVGYTPDVLCGVW
                     IGYDDMRSLGASETGGRTACPVFVDFMVSALRDRQAKDFNVPEGVVFARVDPRTGKVQ
                     QSMDDQSRFEVFKEDSVPVQDEQSSSDQLLKEVF"
     gene            14591..>15257
                     /locus_tag="PLT69_00265"
     CDS             14591..>15257
                     /locus_tag="PLT69_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ12914.1"
                     /translation="MSREYSPQAVETKWQKIWEENNLFRVTEDPSRRKYYLLEMFPYP
                     SGRIHMGHVRNYTIGDVVARYKKMQGFNVLHPMGWDAFGMPAENAAIASGVHPKKWTY
                     ENIAYMKAQLKRMGFSYDWEREISTCDPDYYRWEQLFFIQMFERGLVYRDRTFLNWCD
                     SCKTVLANEQVEDGLCWRCGTQVFLKEHDQWFFKITRYAQKLLDNIERLKEGDMLESS
                     FGEF"
BASE COUNT         2826 a         5066 c         4672 g         2693 t
ORIGIN      
        1 cgatcccctc ggtgagctct cgggaacaga aaatccctgg catgaagccc ctatacccca
       61 ccgaacccac acgtgacaag tgaaagccct tgaaatcgag cgattcaggg cccttttctt
      121 tcttgacatc cattctcccg catgtaaata cttaccgctc aaaatcactc gatctcggag
      181 tacgtcatga agcggacatt tcagcctcac aacaccaggc gcaagcgcac gcacgggttt
      241 ctcgtccgca tgcgcaccaa ggggggaagg ctcgtcataa accggaggag ggcgaagggc
      301 aggaagcgcc tgtctgtctg atgccggacg agaggtttcc cagccgggag cgactgcgga
      361 agagggccga gttcaagcgg gtggaacagt acaagacggc aaggatcaac accaggcacc
      421 tcgtcatcgt cgccgcaccc aacgacctgg aacacacgcg tatcgggata acggcaagca
      481 gaaagatagg gggcgcagtc tcgcggaaca gggtgaagag gctcttgagg gaggtctaca
      541 ggaggaacaa gccgtccttc ccgcgtggac acgacgtcgt cctcatcgca aagaacgaca
      601 tgtcggacat cacctaccac gacctcgcag gcgaggtcac agccgcgctc ggttctctcc
      661 catgggaaag aaagccctca tcgcactgat caggatctac caggcgatcg tctcacccat
      721 gctcggcccg gcctgcaggt ttcacccctc gtgctcggct tacgcgatcg aggccataga
      781 cacccacggc ccgttgcgcg gaacgatcct ggccgcctgg aggattctac gctgcaaccc
      841 cttctgcaag ggggggttcg acccggttcc cggcacgaag acacccggag aatgcacatg
      901 atggatgaaa accagaggac gatgctcgcg ctggcaatca gcgccctgat ccttgtcgct
      961 tggttcgcct tgttcgactc cctgaacatc ggccggaagg aagcccccga gacggctaac
     1021 caggaaaaga aggcgacctc ggccccacag accctccagg ggaaacccgg ccaggcgccg
     1081 ggcaacctgc cggccggccc ggcgcccgtg caggccgctg caccccaaca ggaaacggag
     1141 cgcaccatcg tcgttgacac cgacctctac cgggcaacct tctccacgag gggggccagg
     1201 ctcacctcct tcaagctcaa gaagtacccc atcaccatgg aaaagggctc cgaccccgtc
     1261 gagatgatca aggcccccgt ccccgggctc gacatcgagg ggatagggag cgatgcgggg
     1321 ctcgtcttca cgaccccggc gcgagacagg gtgacgctca acgggtccgg cgggtcgatc
     1381 gagttcagga gggaggtcgc tgccggcgtc accctcgtga agacgttcac cttccagccg
     1441 gggtcctacc tggtcggctt ccgcgtgggc ctttccaacg cgaccggttc ccccgtgcgg
     1501 ctctcgggaa ggctcgtctt cgacagcgtc taccccttga gcgagaagat caagggctat
     1561 gccttccagg gcccggtcct cctgaacgac aaccgcctgg aggagttcaa gatcgacaag
     1621 gtcaagaagc cgggggcgta ccgggaattc accgggagca tcgggtggtt cggggtcgag
     1681 gacaagtact tcctccacac actggtcatc ccgaggccct accagacgac ggtcaccgtg
     1741 aggcgcctcg atgaccgcgt ggtggaactc tcctgcgcgc tgcccaccct cacggtgaaa
     1801 caggccacgg ccgtcgcgat cgaccattcc ctgttcatag gcccgaagga actcaacacc
     1861 ctcaaggcgg cggggaacga gctggcaagg gcgctcgact tcgggttctt cgacttcata
     1921 gcgaaacccc tgctcgtggc gatgaactgg atcaaccgct acaccctgag ctacggcatc
     1981 agcatcatcg tgctcaccgt gatcatcaag atcctgctct acccgctcac cctcaagagc
     2041 ttcaagtcca tgaaggagct gcagaagatc cagccgctca tgaaggagat ccagaaccag
     2101 tacaaggacg acaagcagaa gatgaaccag gagctcatgc gcctctacca ggagcacaag
     2161 atcaacccca tgggaggctg tctccccatg ctcctgcaga tccccatcct cttcgcgctc
     2221 tacagggtgt tctaccaggc catcgagctg aggcacacgc ccttccacat cgtgggcacc
     2281 tggctgccgg acctctccga gaaggacccc tactacatca cgcccatcct gatgggtctc
     2341 acccagttcg tcctccagaa gatgacgcct gcgaccgggg acccgaccca gcagaaggtc
     2401 atgctgatga tgccgatcgt cttcacgttc ctgttcttga acttcccgtc cggcctggtc
     2461 atatactggc tcgtgagcaa cctcctgtcc atcgtgcagc aggcgtacat caaccggaaa
     2521 cacgcatagg agctgtgtta tggaagacca cgcacaggaa ttcgaggcca agaccgtgga
     2581 cgaggccatc atcctggcca tgaaaacgct ccacgcggat ttcgaagacc tcgacataac
     2641 ggtcgtctcg gagggatcga agggcctctt cggcatcgtg ggctcgaaga acgcgaagat
     2701 cctcgcgcgc aagaagggcc cgggctcaag gccgggaacc gacagggtcg tcccgccgcc
     2761 ggaggccccg gccccgcggc agccggtgga cacaggccgc cccggctcac ccgacaccca
     2821 ggcggccccg gcgcaagacg tcctcgagca ggcaaaggag gtcgtctcga ggatactcgg
     2881 gctcatcggg ctgagagcgg aggtcaggcc tcgagacgac gggatgctgg agatcgtcgg
     2941 cgacgggtcc ggcatcatca tcggcaagca cggccagacc ctggacgccc tgcagttcat
     3001 cgtgaaccgc attgtcaaca agtcgaggca ggaccaggtc cacgtcacca tcgacaccga
     3061 agggtaccgc aagaggcaca tcgaccacct gaaggccctg gcgatcaaga tgggccagaa
     3121 ggcgaagaag accgggcacc cggtaaccct agagaagatg aacccttacg agcggaggat
     3181 catccacctc gccctcaagg atgagcgctt cctggacacc aagagcatcg gcgagggggt
     3241 gtacaagagg gtcatgatcg tccccaagag ggcctcgagg cgataaggat cgaagacacc
     3301 atctatgccg agtccacacc gcacggcacg ggcggcataa gcctcgtgcg catcagcgga
     3361 cccaacacgt ccgccgtcct ctcgaggatc tgctcgaggc gctccttcgc cccccacgtg
     3421 cagcacctca ccgtcttgcg ggacgaggcg ggcgccaccg tggaccaggt caccgtcgtg
     3481 ttcaacccac aaccacgctc gtacacgggc gaagatctcg cggagatcgg ctgccacgcc
     3541 aaccccatcg tggtggaacg catcctgggg gtcatagcat ccacggggct cgccaggcgg
     3601 gccgcccccg gcgagttcac catgcgggcg tttctgaacg ggaagatgga cctcgtccag
     3661 gccgaggcgg tgagggcctt catcgagtcg acgaccgacg cggggtgcga catggcgcgc
     3721 aggctcctag agggcgggct ctcctcgagg atcaagaaac tgagggcctc gatctcggag
     3781 atcctcgcgg acatcgaggc gtccttcatc tccgaggacg aggtcgtcgc ggcggaggag
     3841 ctcgcggcga ggatcgagcc cctggcggca gagcttgcgg ggatgtcggg agacgcatgc
     3901 cgcggcctca aggtctacgg cggggtcacc acgaccatcg cggggctccc aatgtgggga
     3961 agtcgtccct cttcaacgcc atcgtgggca gcgaccgggc catcgtccac cacgaggagg
     4021 ggacgaccag ggacgtgatc agggagcgcg tcaccatcga cgggatggac ttcctcttcc
     4081 acgacacggc cggcatcagg accacgccct cggggccgga acaggccggg gtggaacggg
     4141 cgatcgcggc ggtgaaggca tctgacctcc tcctctacgt cgtggacgcg cgcgtggggc
     4201 tccagccgca cgagtcacag tggctggggc tcggcgggac cacgatcctg gtggtgaaca
     4261 agatcgacct cgtgaaggag ccggcgatca ccgagacagg gtatcgcacg gtgatggtat
     4321 ctgccaagca ttccaccggg atcgacgcgc tcaagtcggc catggtggag acgttcaggc
     4381 cggcgaaccc ggaggtgttc ctggacaggc acgcccacct cctctcggag gcatcccggc
     4441 acctcgactc gtgcctctcg gccctgtccc aggggatgac cctggacgtg gcgtcgatcg
     4501 agctgtcggg cgcgctgcgc gcgctcggcg cgatcacggg cgagtcggcg gacatggaca
     4561 tcctggagca ggtcttctcg aggttctgca taggaaagtg acatggatga ccgtctcgcc
     4621 aagtggatgg gggaactcag gagattcaac cccaggctgc acctggttgg cccctccatg
     4681 ctcgagcacc tcgaggagga ggtcgaggtc acgtcccgtc ttctcgaggg catcaccgag
     4741 ccggcgatcg cggatctcgg ttccggttcg gggctcatgg ccttcgtgta caaggcgctc
     4801 caccccgact ccatcgtgac gtgcgtcgag aggagcgtga agaagtgcga cttcttgagg
     4861 cacaccgcgg aggtcctcgg cctcgagggt ttctgtgtcc tgtgcgagga cctgctcgcg
     4921 agcgggcgcc ggttcaaggc ggccatggcc cgctcgttct cgccggttgc gaccctcggg
     4981 aagatatgct cgagggtgct tgtcgagggg gggaggctct actacctctc ctcgtcgaac
     5041 ccgcaggaga tgggaagcgg cttcaggctc gacggcatcc agacggcccg gtcgggcacc
     5101 gggctgaccc tcaacctttg gagattcacc cggcttcgcc cgtgacgggt atcttcctgg
     5161 acaccctggt ttccagcctg ggcagcgata tctcgaggac acctgcgttg aagctggcct
     5221 ccacccggtc gaggtcgatc gactcgggca ggctgaacga cctcctgaac agaccgtact
     5281 ggcgctccac caggtggaag ttctccgctg cgtccacgtg atcgcacggc ttggtgccgc
     5341 tgatggtgag caccccgtcc gtgatgtcca gcgcgatgtc ctcccgcctg acccccggca
     5401 actcgatctt gacgtagagg gtcgagtcgt cctcgtatat gtccacgcag ggcacgaacc
     5461 tcccaggccc tgccaccatg acgctgtcct gcatgctctc ctcgaacgcc ctgctcatcc
     5521 tctccctcag aaagaggagg tcgcccctga gcgagtgtga cgacgccatg gcccgtaccg
     5581 cctcactgca gcatgagctc gaggttcccg gcgagcgcct tcttgagctt ccccttggct
     5641 gcgttctcga tctgcctcac gcgctccttg gagatcgaga agcgcctgcc gagatcctcg
     5701 agggtcatgg gctgctcggc catgagcctg tgcctcgcga tctcccgctc acgctctgtg
     5761 agcttggcga gggcggactc tatctgcctt ttcacgaacg cagacctctg cgactcgatc
     5821 accctgtcct cggggcccgg cctcgtgtcg gcgagaaagt cgaggaagct cgaatcgctg
     5881 tcttccgcca tgggcgtgtc gagggactgg tctctccccg tgagcctgga gatgatctcc
     5941 tcgatcctcc tcgggtcggc gtcgagccgc gcggagagcg cccttgactg ctccccgata
     6001 ggttcgtggt tcacctcctc ctcgctggac atgcggttca ggccgtagaa gagcttcttc
     6061 tgcagcttgg tcgtcccgag cttgacctgc gagaagaacc tcaggatgta gtcgtggatc
     6121 atcgccttga tccaccacac cgcgtacgtc atgagcctgt aacccttgtg ggggtcatac
     6181 ttcttgaccg cgatcatgag cccgatggtg ccttcctgga tgatgtcctc gaggggcagg
     6241 ccgtaccccg cgtaatccat cgcgatccgg accacgttgc ggaggttcgc ggtgatgagc
     6301 ctgtgggccg cgtccacgtc cccctgttcc ctgagcctca cggcgagctc gaactcctcc
     6361 tccttggtga gcgctgggat ctggtcgaac ttggcgacca gggcagccac ttcatgatgg
     6421 taaggtactg gaagatgcat gtctccactc ctttgttttc tgaaccgccc atgaaaaatc
     6481 tcaatgaaaa tctatcggtt cagccggtcc gtaattttag atgaaaaaaa gggttgaagg
     6541 tttcacccga tccggggtat aggtctgccc attcggggag acatgctatg atgtcattca
     6601 gggcagtgat catctccggt gtcgtattcg ctgtcatggg gtgctcatcg gggctccact
     6661 acacctaccc cccgccacac cacccgagtc ctgtactgga cctggccaag gtacaggaca
     6721 tgcaggaaac gaaacccagg gaccatcacc aaccacccca ccaatcacca gcaatcccca
     6781 ccacggaaga gaccaaggcc atccccaggg ggaacctcgc ctctctcggc gtggcaccgg
     6841 accaatcggt ttcccgacac ccggcctcat ccagcccgga ggacgagcac gatcgagccc
     6901 cggcgggcag gggcgagatt cgggacacga ccacggccgg tgaggccttg ccggacggga
     6961 cgtggcttcc cgcaggcggc ctgaggccca gggtgaacgc ctggatcgag cggctcacgg
     7021 gcaaggaaaa agacacgttc tccagccagc tggcgaggct cgagaggatc cggccgacca
     7081 tcgagcgcat cttctccgaa cagggggtgc ccgccgaact ctcctacctc tgcctcgtgg
     7141 agagcggcgc caggccggac gccatgtcgc cctcaggcgc tgtgggctac tggcagttca
     7201 tgccggacac cgcccggagg ttcgggctcc aggtgaacag gtacgtggac gaacgaaaga
     7261 acctcgagaa gtcgaccctg gctgccgcgt cgtacctcaa gcacctctac gccatgttcg
     7321 gggactggta cctggccata gcggcctaca atgccggcga agggaccgtc atgcgcctca
     7381 tgcaggccca ccggttacgc tcctactggg acatcgacgc cgggatggcc atcaagtacg
     7441 agaccatcga cttcgtaccc aagtacatcg ccacggtcat catcgcgcac acctatgagc
     7501 ggtacgggct gaaaccaccc tcttcgccct tgagccgcgg tctcgggatc gcctctgggg
     7561 ggtatctccc ggggtccgag ccgtacgggc agtcctcgcg gtcccgcatg gccctggcac
     7621 cgcctctggc cgtcaccgac gcacccctgg gaaccaggac ctcatcggag cgcgacgccc
     7681 cccaggcggg cgacacaacg gcggacgctg ggggacggga ttgcttgcgg cccctgaccc
     7741 acaccgtgaa aaagggcgat accctctact cgctcgcgaa gaggtacgat accacggtga
     7801 agtccttgtg cgaggccaac cacctgaaat cctccaggca catccacccg ggcatgagac
     7861 tcgagatccc cgcggccgga aagacccctc cccgggggac acctgaccgg gaaccccggg
     7921 tgagcggcgc cgcacagatg aaggcgaaag gcgggccggc ccgcgaagga aagaacaccc
     7981 cgacctacac cgtgaagaag ggcgacaccc tctggtccgt ggcccgccgc tacgaagtga
     8041 ggcctgccga cctggcggca tggaacgaca tcgggcccga cggcgtcatc aagcctggag
     8101 aacggctggt gatccgcccc aggtgaaccc gggcggaaag gcctcacggc gatccagaaa
     8161 cctcgacggg tctccccggt gcggtcggga ccgcccggta ggctcgcttt gaagatgcag
     8221 agggcggacg tttccctgtc cccgcccagg aaagcaggat accccgatgc aaaggccgct
     8281 ggagccggtg tcctgccgat ccctcgtgaa caggggggaa ccgcgttcgt gaatccgtaa
     8341 gcgcggaatg ggaggcgccg gacctgctcg caggcgagcg cccccctgtg cgggagctgc
     8401 gggcacactc ccggtctcag gcgaagccgg tcgagaaccc tcacggtctc ctgcaggatc
     8461 gatcgccccg gcgttggcgt cgtcgtctcc ggttttcttt cgatgcgggc ttcggatact
     8521 tcgagccagt tcttcctccc gtcgtcctca ttcacgttct cggctttctc tccctttgcg
     8581 gtgaactctt cccgcctttg gacgcccggc catcagccgc acgcctcacc cccttcccgc
     8641 cgggaccggg atcgggacgc aaccacaccg caaagccttt tcttttcacc cttcaggatg
     8701 tattacaacc atgggaaggg ccggaaggtc cgggtacgcc cgtctcggac ccaggaaggc
     8761 ccggggagga ccagatgaag aagttgtctt tcgccctcgc atgcatgtgc accctcgccc
     8821 tgacgatcgg cgccccgcgg cacctccagg gggaagccac gtggtcgcag gcctcgtatg
     8881 tgaccgacga ggacctgttc tgtgtgaccg ggtggtcgga cggcgagatc tatgcctgcg
     8941 gggaggacgg ggagctcgtc ggtttcgacg gggtgtcctg ggacgagtac cctgtcccca
     9001 gggccttcga tctcagggcg atcgccctgc tcgcaacggg cgacctcctc gtcacgggcg
     9061 acatggggat cgtgctcacg ggttcccccg ggggggtcct ggccgaggcg taccccgtgc
     9121 tgacggatct caacgccctg tggctctctt ctgacgacgc cgtgatcacc tgcggtgatc
     9181 tcgggaaggt gctcgtctcg gacggcgact cgtggagcga catgagcccc gaaaacgccc
     9241 aggcgttcga cctctactgc gcgtggggca ccggggagga ggacttctgg gtcggcggct
     9301 ccttcggacg tctctggcac taccgttcgg ggagatgggc gtcatatccc atgaccgacc
     9361 tcaccacgca gaccttcagg gccctgtggg gcgcatccga ggacgccgtg ttcgctgcgg
     9421 ggacgctcgg ccacatcctc gcctacgacg gatcgtcgtg gtccatcgtg cacacgaccg
     9481 acgggctcgc cgtctacgcc ctctggggca cctcggacga cgacatcttc gcatccgggg
     9541 agatggggtt cgtgtaccac tacgacggcg gcacctggag ccgtatccgc agccccggca
     9601 ccctggacat ctacgcgatc tggggcggga cctctggcag ggtgtatttc gtgggtgaga
     9661 atggcctgtt cctcgtgtac tccagggaag acacgtacgc cccgtccgtg gtgcacacaa
     9721 gccccacagg ggggtccacc ggcgtaccga gggatacgtc cgtggtcttc gtgctgagcg
     9781 aggaagtcga tccctcgacc gtcgacaacg cctcggtcag gctcactgac gggaaccaag
     9841 aagtgcaggc gagggtctcg agctcggcga acagggtgac cctgacaccg gaggaggccc
     9901 tcgagccatc cacccgctac accgcgacgt tgtcccagca ggtggcggac ccgagcggga
     9961 accgcctcgg gcaggcctac accatcagct tcaccaccgc tgcaaccacc ggggaagacg
    10021 atgggggcgg gggctctggc tgcttcgtgg cgtgctgctc ggcgtactga ccgaagcggg
    10081 gaaagaccac gtgtaaaaaa tcatgcggag cgcttctccc cgacaagggg aatccccgca
    10141 aacccggcct ctgggcgcca agaccgcggg cagccttttg cagccgtctt cgggttcgtg
    10201 cggccggcat cccgggcggc ctgccgttcg cgcgcaacgc ctggacaagc aggagaaggc
    10261 ctcctcgggc ttccttcgca cccgggggct cgttcggccc taagccacgg cgccgggctg
    10321 aatcgccgga gcacctcgaa cgtccgggag aaggccgagg caggttcctc aacgtctcac
    10381 ggaaggcttg gcctgcccac cgctcaccac acgatcagcc tgtagccgag cgatgcgata
    10441 tactgctgga tcacctggtc gatcctgctc aataggatca tggtggcgcc ggtcttgaca
    10501 gccgggatct cgagctcggc ctttccctgg gccaccttga gcctgacggg cggcccctcg
    10561 agggtgaagc cgaggcccgc caggacctcc tgggggctcg cgcgagaccc gtcgaacacc
    10621 tggaacgacc gcttcgagag caaggacgcg gtctgggcat ccggtgaccc atcggccacg
    10681 atcaccctgc cggacaccac aggggccttg tacacgatgc gcacgggtcc gccggagacg
    10741 agctcgatct ccttgtccct ctcgaagggt atggacaggg ccttgaacag ctccctgtag
    10801 gaaccgctca cgctcaccgg cgccatgggc ctgtccgtac gcaccctcgg ctcgacgccc
    10861 ccgatctcga cgatgtcgat gccgaaagcc tggacgtagc gcctgatggg ctgtgggatc
    10921 ttgctgtccc tctcgccgag gaggttgatc acgtacacgt tcctccgcga aaagtccttg
    10981 tatacaagcc acttgccgaa cagggagacc ttctcctccg agccgaccat caagggggcc
    11041 gggtccttgt tgaccgagaa gaacccggac acgctcagga ccctgtcaat gagactctcc
    11101 atgtccgcct cgggcccgct gacaacggag atcgacggga aggccttctc tatgtacgat
    11161 ctcacctcgg gggtgatctt cccctccagg tcgaggatga tcttcttgcc ggtgtcgagt
    11221 tccatcacgg ggatctcccg ggtgtctatg gccatgccgg ccccacccgt gaccgggacg
    11281 tagtacgtgc ccgtgccctg actggacccc cccattgcct ccagggcggg aaggagtttc
    11341 cccttcacgt ctttggcccg cgcgaccctg cctgtttcgt ttgtggcccc tgtcgcctgg
    11401 tccgcacccg ctcgcggccc tgcgtcttgc ccgccgtttt cccgatcgac cgcaagggcc
    11461 tcgtcccctg caggacccgc cttccggccg ttctttccct ggccctgctt cgaatccgcg
    11521 aggatctgtc tcacgctcgg gatccgtatc ttctggcccg cgaggatcct gttggggtcg
    11581 gtgatctggg gattgagcct cttcaccatc tcgatgtacc tgctgaacac gagatcgtcg
    11641 ggcaccccgt agatctcctt caggatcttg gagaggtact ccccctgcct caccacgtac
    11701 tcctcggcgg agacctcttc cacccccagc cgcccgctcg gcgtgcctgc aggcgcgcgc
    11761 tccctccctg ccaccgagtt tacgggtatc tttatggact tgcccaccat gagggaatcg
    11821 aggtccgcta tgtccgggtt caactgcctg aatcgggcgt agaggtctgg aagatcgttt
    11881 tcccgagccc ggtatacctc catgaggatc cttcgcaggg tgtcaccctg cttgacgacg
    11941 taggtgtcgc cgggcggggg tgcgatacgc ctcgcgtgtt tgaccagggt gatgtcctgt
    12001 gcatggggag acacagggga cagcaggacc gccgcgagga aggccgcaat gccccaccgc
    12061 attcagcaga tccccgcaag cggtatcatg agacgaccct ttcggggcaa ccagaccgca
    12121 agacttccct gcattcagaa gacctccttg aggagctgat ccgaactgct ctgctcgtcc
    12181 tgcacgggca cggaatcctc cttgaagacc tcgaaccgcg actggtcgtc catggattgc
    12241 tgtacctttc ccgtccgtgg atccacccgc gcgaacacca ccccctccgg gacgttgaaa
    12301 tccttcgcct gcctgtccct gagggcgctc accatgaagt ccacgaacac ggggcacgcc
    12361 gtcctgcccc ctgtctcgct ggcacccagc gacctcatgt cgtcgtaccc tatccacaca
    12421 ccgcacagga cgtctggcgt gtaccccacg aaccacgtgt cgcggctgtc gtcgctggtc
    12481 cccgtcttcc ctgccaccgg cctccccaga gccttcgccc tccacccggt accctccctc
    12541 accacgtcct tgaggatgtc ggtgacgatg taggccgtct ggggcgatat gacctgctcg
    12601 tacacggggc cccccggctc gcctgtttgg gccacttgcc tgatgggcgg gggggcctgc
    12661 aggatcgtcc ccttgccgga ctggacgacg ctctcgatga ggtggggctc gaatctccgc
    12721 ccatagagcg cgaataccgc gtaccccttg aagaggttgt acggggtgac gtccgctgag
    12781 ccgagggcta tggagaggtt gcgggggagc ttcgtcttca tgtcgaagcg gtcgatgtat
    12841 tccctcgcgt agtcgatgcc gatctccccg aggatcttga tggtcaccac gttcctcgac
    12901 agcacgaggc ctgtcctgag cgacgtggcg ccgaagaact tgttctcgaa gttcttcggc
    12961 tgccatggct cctcgtcagc accggcgttg tagctcaccg gcgcgtcgaa gacgatggtc
    13021 gcgggtgtca tccccttgtc gatggccgcg gcgtagatga agggcttgat cgcgctgccg
    13081 ctctgcctct tcgcgaacac cgccctgttg aactggctct gggaatagtc caccccgccc
    13141 accatcgcaa gtatgccgcc cgtgttcagg tcgaacgcga ggagcacccc ttggaccgtc
    13201 ggcttctggg atatcacgaa cccctgcggg gtgtagcaca ccatgatgag gtcacccggt
    13261 ctcaggtgct tcgccgggga ccacctgccc ttcgggttta tccactcgta actcgcgggc
    13321 cccagctcga ccctcctggt cccgagcttc acgaccaggg gttccaccga cacgacctcc
    13381 gcccagtaca gctcgtaggg ctcgtacccc ttccactcga tctggttggt ctggaacgag
    13441 aacaacctct ccttgtcctc tatcgtgaag ggcttcatgg aaccgtccgg ctggatgacg
    13501 ccccggacgg gaccggcgta cttcccctgc ctcatctcga gctccatgac gcccttgcgg
    13561 accgcccggt acgccgcccg ttggagcgag gggacgatgg acgtccgcac gactatgccc
    13621 ctgtcgaaga tctcctcccc cagctgctgg accacgagcg tccgggcgta ttccgtgacg
    13681 tacggctcct ccgtgaacag cgccgattcc ttggccacta tcctcagcgg ttctccgtac
    13741 gccgccctcg cctcttcctc ggtgatgtag ccctcctcaa ccatgcggtt caggacgtac
    13801 cactgcctcc tcttgcacgc ggtgaaatcg ttcaggggcg agtaacgcgc aggggccggc
    13861 gcgagacccg cgatcatggc catctccgag agctcgagct cccggcactc cttgccgaaa
    13921 tagcgcaggc tcgccgcctg cacgccgtag gccccgctgc cgagatagac cctgtcgagg
    13981 tagaggttca ggatctgggg tttggtcagc gcccgctcga tcctgagggc gagcacgatc
    14041 tccttgatct tcctggtcag ggtcttctcc ctggtgagga ggtacgacct gacgacctgc
    14101 tgggtgatgg tggaagcccc ctgcacgaac gagttggccc ggatgtccgc gacgagggcg
    14161 ctcaggatcc tgcgcggctc gaggcctatg tggtcgtaga acgtcgagtc ctcggccgcg
    14221 atgatggcct gcttcaaggt gagggggatg ccgtggccgt tcatcacgac cctgccctcg
    14281 ggtatgaagt agcccaggac cgttccatcc tcggccgtca ccttgctcac cctcgtcgac
    14341 tcgggggcct tgaggtcctc tatggagggg aggtcccgga agataatgag gtacgtccac
    14401 agcgagacga agcataccgt gatgaggaga gccagcagga cgaacttgag ccatctcata
    14461 gtgattcctt ttctatcagc ccatgcacga aatgcaagcg ccaatacccc cttcaagggt
    14521 tcgagcgctc tggactcgca agaccccgat atggtaatga tgcggcaacc atcgcacagg
    14581 aggcgccagg atgtcccgcg agtacagtcc gcaggccgtt gagaccaagt ggcagaagat
    14641 atgggaagag aacaacctct tcagggtcac cgaggacccc tcccgcagga agtactacct
    14701 cctcgagatg ttcccgtatc cctccggccg catccacatg ggtcacgtgc gcaattacac
    14761 gataggcgac gtggtcgccc gttacaagaa gatgcagggt ttcaacgtgc tccaccccat
    14821 gggctgggac gccttcggca tgccggccga gaacgccgcc atcgcgagcg gggtccaccc
    14881 caagaagtgg acgtacgaga acatcgccta catgaaggct cagctcaagc gcatggggtt
    14941 cagctacgac tgggaacgcg agatctccac ctgcgacccg gactactaca ggtgggaaca
    15001 gctcttcttc atccagatgt tcgagagggg gctcgtctac agggacagga ccttcctcaa
    15061 ctggtgcgac agctgcaaga cggtcctcgc gaacgagcag gtggaggacg gcctgtgctg
    15121 gcgctgcggc acccaggtct tcctgaagga gcacgaccag tggttcttca agatcacgag
    15181 gtacgcccag aagctgctcg acaacatcga gcggctgaag gaaggcgaca tgctggagtc
    15241 cagcttcggc gagttcg
//
LOCUS       DAOSCR010000004        14839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_2744_length_14839_cov_3.581913, whole genome shotgun sequence.
ACCESSION   DAOSCR010000004 DAOSCR010000000
VERSION     DAOSCR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2744_length_14839_cov_3.581913"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(792..1604)
                     /locus_tag="PLT69_00270"
     CDS             complement(792..1604)
                     /locus_tag="PLT69_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ12915.1"
                     /translation="MQPAEVTPAVTLAGVTFSYPQTLALAGIDLEVPSGSYCGIVGPN
                     GSGKTTLAYLISGVLKPTSGTVDTKGRRVGLVLGNPANQIVSLVVEEDVAFGPENLGL
                     PAFEIDRRVTRSLEAVHCQHLRHSLTSELSGGQLSKIVYAGQLAMEVDVLVLDEGTAM
                     LDPASRSQVLDQIRELNATLKTTVIHITHRLDDLSAADMVLVMVGGKIVHRATGAIEL
                     ARKASELAGAGVEAGHEIVYRCFLADMGIEEEDLEKATELLAERLRQARRCR"
     gene            complement(1604..2068)
                     /gene="rplQ"
                     /locus_tag="PLT69_00275"
     CDS             complement(1604..2068)
                     /gene="rplQ"
                     /locus_tag="PLT69_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOJ12916.1"
                     /translation="MYHRIAGRKLQRTTDHRQALLRNLVTSLVMNERLTTTEAKAKEL
                     RKLADWMITLGKKGDLASIRRAERVLRSKAAIGKLFKELAPLFANRNGGYTRIIRTSV
                     RRGDHAPMAIIEWTEKKAAEKSPGKEKKEPSRKGLFSRKKEKKAEKAPEAED"
     gene            complement(2085..3122)
                     /locus_tag="PLT69_00280"
     CDS             complement(2085..3122)
                     /locus_tag="PLT69_00280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ12917.1"
                     /translation="MIEKNWKEIIKPTKLEIVEETYSDTYARFNAQPLERGFGVTIGN
                     SLRRILLSSIYGSAIIGVRFDESLGVLHEFSSIPGVYEDVTDIILNLKKVVMRMHTDK
                     PQTVTLEKKGPCEVKAGDIDTAGKMDVINPDQLIATIQTDLTFKLQLRVDWGRGYVPA
                     ELNKEKLDVHGWIPVDSVFSPVRRVSFNVTPTIVGRRTDYDRLSLEVWTNGVVTPDDA
                     LAYAARIQKEYMDVFINFHEEEIEHMGEEEGPEQELNDNLFRTVEELELSVRSANCLK
                     NANMSYIYELVQKSESELLKTKNFGRKSLEEIKEKLAKMGLHIGMKLPNLPSPEEIEA
                     RRRRMEEEESK"
     gene            complement(3146..3772)
                     /gene="rpsD"
                     /locus_tag="PLT69_00285"
     CDS             complement(3146..3772)
                     /gene="rpsD"
                     /locus_tag="PLT69_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOJ12918.1"
                     /translation="MSRYTGAVCKICRREGQKLFLKGERCYTDKCAIEQRAYPPGQHG
                     QGRVRVTEYRLHLREKQKVRYTYGVSESQFRRYYDMAKRTRGVTGEQLLQLLERRLDN
                     IVYRMGFSSSRNAARQLINHGHILVNGRKVDIPSYLVKAGDVVEVKEKSKKMESIGAS
                     LENREQRGFAEWLEVDKEARRGRIKALPERKDMPTSIQETLIIEFYSK"
     gene            complement(3791..4183)
                     /gene="rpsK"
                     /locus_tag="PLT69_00290"
     CDS             complement(3791..4183)
                     /gene="rpsK"
                     /locus_tag="PLT69_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOJ12919.1"
                     /translation="MAKARQRTGRKRVRKDVPEGVVHIQATFNNTIITITDPQGNTLA
                     WSSAGAQGFKGSRKSTPFAAQVAAQEACKKAQEFGLKSAFAYIKGPGSGRESALRAIN
                     ASGIRITGIRDVTPIPHNGCRPPKRRRV"
     gene            complement(4208..4576)
                     /gene="rpsM"
                     /locus_tag="PLT69_00295"
     CDS             complement(4208..4576)
                     /gene="rpsM"
                     /locus_tag="PLT69_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOJ12920.1"
                     /translation="MARIAGVDLPRNKRIDIALTYIYGIGRTRASEIVRAVKVDPAKK
                     TDELSEHDIAAIRDYIDKNLTVEGDLRREVSMNIRRLMDIASYRGIRHRKGLPVRGQN
                     THNNARTRKGPRRLVVGKKK"
     gene            complement(4592..4705)
                     /gene="rpmJ"
                     /locus_tag="PLT69_00300"
     CDS             complement(4592..4705)
                     /gene="rpmJ"
                     /locus_tag="PLT69_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOJ12921.1"
                     /translation="MKVSASIKKRCRKCKIIRRSGVVRVICENPRHNQRQG"
     gene            complement(4719..4934)
                     /gene="infA"
                     /locus_tag="PLT69_00305"
     CDS             complement(4719..4934)
                     /gene="infA"
                     /locus_tag="PLT69_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOJ12922.1"
                     /translation="MAKDVIEAEGIVVEPLPNAMFRVELANGHQILAHISGKMRMHYI
                     RILPGDKVQVEISPYDLTKGRIVYRSK"
     gene            complement(4948..5592)
                     /locus_tag="PLT69_00310"
     CDS             complement(4948..5592)
                     /locus_tag="PLT69_00310"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOJ12923.1"
                     /translation="MNIILIGPPGAGKGTQAKRMIDRLGVPQISTGDMFRAAVKEGTP
                     MGLKAKEYMDKGALVPDEVVIGVVKERFGKPDCAKGFILDGFPRTLEQAKALDGLLKG
                     MGKSLDHVVVIEVPDDYLVKRLTGRRTCRGCNYMHHVEFDPPKKEGVCDKCGGELYQR
                     DDDQEATIRSRLKTYHDQTSPLIDYYSKQGVVRTIDGTKSMEQVQEAIQKAIGA"
     gene            complement(5622..6935)
                     /gene="secY"
                     /locus_tag="PLT69_00315"
     CDS             complement(5622..6935)
                     /gene="secY"
                     /locus_tag="PLT69_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOJ12924.1"
                     /translation="MANGYGFSNIAKVPELWNRILFTLAMLLVYRLGIHIPTPGIDSA
                     IMKEWFDQQQGTLLGMFDMFSGGGLRGLSIFALGVMPYISASIIVQLLCMIYPPFEQL
                     QKEGATGKHKLTVYTRYLTVLLCLIQSHFMAFGVEQMTVAGQSVVSNPGWGFRMVAMI
                     TMTTGTCFLMWLGEQITERGIGNGISLIIFAGIVARLPLAIINTFRLVRTGEMSIIAL
                     ILVVVLVAVVIAGIVYFETSQRRIPIQYPKQIKGRRIYGGQSTHLPLKINISGVIPPI
                     FASSIIVFPATIAQFFPDVGFMKTLAGALSPTGFWYNVIYVIAIVFFTYFYTAVIFNP
                     LDVAENLKKNGGYIPGIRPGKATAEYLDRVLSRLTFIGAIYLSIVCVLPMILISWTNA
                     PFYFGGTSLLIVIGVSLDTMSQIESHLITRQYDGLLKTGRIRSRR"
     gene            complement(6946..7386)
                     /gene="rplO"
                     /locus_tag="PLT69_00320"
     CDS             complement(6946..7386)
                     /gene="rplO"
                     /locus_tag="PLT69_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006736157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOJ12925.1"
                     /translation="MDLSNLKPADGSRKKKKRVGRGNSSGMGTTCGRGTKGQKARSGG
                     NVPQWFEGGQMPIYRRLPKRGFKNRTRIEYAVVNVKDLARFEKGSVVDRDALRMRGLI
                     PSLSVPVKLLADGEIDVPLTVRLDKASSAAIEKISRAGGTFEHI"
     gene            complement(7391..7573)
                     /gene="rpmD"
                     /locus_tag="PLT69_00325"
     CDS             complement(7391..7573)
                     /gene="rpmD"
                     /locus_tag="PLT69_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOJ12926.1"
                     /translation="MAGKIKVTLYRSVIGCTEKQKAIVAALGLKRRGRSRVLPDNPAT
                     RGAVAKVPHLVKWEEA"
     gene            complement(7599..8105)
                     /gene="rpsE"
                     /locus_tag="PLT69_00330"
     CDS             complement(7599..8105)
                     /gene="rpsE"
                     /locus_tag="PLT69_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOJ12927.1"
                     /translation="MARFEQIDENQEQLIDKVVYINRVAKVVKGGKRFRFSALVVVGD
                     GNGKVGHGLGKAKEVSVSIRKGYESARKAMRDVPIVSGTVPHEIIGEFGSSRVIVKPA
                     PEGTGIIAGNAVRAVMEAAGYRNVVAKTLGSRNHNNVIRATFDALDRLETREYVEAKR
                     GKTLDLGS"
     gene            complement(8123..8485)
                     /gene="rplR"
                     /locus_tag="PLT69_00335"
     CDS             complement(8123..8485)
                     /gene="rplR"
                     /locus_tag="PLT69_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOJ12928.1"
                     /translation="MSKKTERQAWQLRKERIRKRITGTADRPRLSVYRGLRHIYTQII
                     DDENGVTLAAASTLSPEIKGKVKGANIAAARLVGELLAAKARDKGIDKVVFDRSGYRY
                     HGRVKAVAEAAREGGLKF"
     gene            complement(8518..9060)
                     /gene="rplF"
                     /locus_tag="PLT69_00340"
     CDS             complement(8518..9060)
                     /gene="rplF"
                     /locus_tag="PLT69_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOJ12929.1"
                     /translation="MSRIGKLPIPIPKGVSVTFDGETLTVKGPKGELKKRCVGNLDVV
                     IEEKELRFVPKDEERKTKAFHGLMRALANNMVKGVSEGFTRRLRIVGIGYRGEAKGNT
                     LVLNVGYSHPVEFRLPEGVSGTVSKDGLIEITGIDNELLGDVAARIRKIRKPDSYAGK
                     GIRYDGEQVRLKPGKTAAKG"
     gene            complement(9077..9469)
                     /gene="rpsH"
                     /locus_tag="PLT69_00345"
     CDS             complement(9077..9469)
                     /gene="rpsH"
                     /locus_tag="PLT69_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOJ12930.1"
                     /translation="MHTDPIADMITRIRNAAGAQKESVDIPASKMKIAIADILQKEGY
                     IKSYRVIDDGRQGMLRVSLKYFNSRHVISGIERVSKPGRRIYRGAAEIEKVRGGLGIA
                     IVSTSEGIMTDHNARAKNKGGEVLLRVW"
     gene            complement(9483..9668)
                     /locus_tag="PLT69_00350"
     CDS             complement(9483..9668)
                     /locus_tag="PLT69_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOJ12931.1"
                     /translation="MAKKSMIAKQRRKPKFKVRQYNRCQLCGRPHGYYRKFGICRICL
                     RNHAMRGEIPGVKKASW"
     gene            complement(9682..10221)
                     /gene="rplE"
                     /locus_tag="PLT69_00355"
     CDS             complement(9682..10221)
                     /gene="rplE"
                     /locus_tag="PLT69_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOJ12932.1"
                     /translation="MARLKEYYLKEIVPALRTEYGYKNVHQVPKLVKIVLNMGLGDAV
                     QDIKIIDRAAEELALITGQKPVVRKARKSIAAFKLREGMPVGCSVTLRGERMYEFLDR
                     LINAVLPRVRDFRGVSPKGFDGRGNYTMGLNDQSVFPEINYDAIDKLRGMNITIVTTA
                     GSDDEGRSLLAAFGMPFRK"
     gene            complement(10231..10566)
                     /gene="rplX"
                     /locus_tag="PLT69_00360"
     CDS             complement(10231..10566)
                     /gene="rplX"
                     /locus_tag="PLT69_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOJ12933.1"
                     /translation="MAVVRHETGRIRKGDTVIVLTGKNKGKQGQVIRVVPKKDRVVVE
                     RVNMIKRHTRPSQKSQGGIVEKEAPIHLSNVAIVCPACKKAVRIGFKFHEDGTKVRYC
                     RSCSEELDK"
     gene            complement(10576..10944)
                     /gene="rplN"
                     /locus_tag="PLT69_00365"
     CDS             complement(10576..10944)
                     /gene="rplN"
                     /locus_tag="PLT69_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOJ12934.1"
                     /translation="MIQQQTRLKVADNSGAKEVMCIKVLGGTRRRYASLGDIIVVSVK
                     DAIPRSKVKKGDVVRAVVVRTTRTHKRPDGTSIRFDENSAVILNNQNEPVGTRIFGPV
                     ARELRSKNFMKIVSLAPEVL"
     gene            complement(10976..11233)
                     /gene="rpsQ"
                     /locus_tag="PLT69_00370"
     CDS             complement(10976..11233)
                     /gene="rpsQ"
                     /locus_tag="PLT69_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOJ12935.1"
                     /translation="MTEQARKLEFEGIVTSDKMDKTVVVTVERLEKHPVFGKYVRRRA
                     KFKAHDERNECRPGDRVLIRECRPISREKCWRVVKILERQE"
     gene            complement(11230..11418)
                     /gene="rpmC"
                     /locus_tag="PLT69_00375"
     CDS             complement(11230..11418)
                     /gene="rpmC"
                     /locus_tag="PLT69_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOJ12936.1"
                     /translation="MKAKEIREKSPAELSELLLKLERELLDARFKKATGQLKDTSIIS
                     KLRRDIARINTIARERVQ"
     gene            complement(11418..11831)
                     /gene="rplP"
                     /locus_tag="PLT69_00380"
     CDS             complement(11418..11831)
                     /gene="rplP"
                     /locus_tag="PLT69_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOJ12937.1"
                     /translation="MLMPNKVKHRKQQRGRLTGSPERGSRVSFGTYGLQSLEPHWITA
                     RQIESARIAITRSIKRGGKVWIRVFPDKPITKKPAETRMGKGKGAPEEWVAPVRPGRI
                     LFEIDGVTEEQAKEAFRLASHKLPVKTRFVVREGV"
     gene            complement(11846..12493)
                     /gene="rpsC"
                     /locus_tag="PLT69_00385"
     CDS             complement(11846..12493)
                     /gene="rpsC"
                     /locus_tag="PLT69_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOJ12938.1"
                     /translation="MGQKIHPYGFRLGVTKDWKAKWYADKGFGELLVEDRKIRDFITK
                     NLSHAGISNIIIERAAGKVKLVISTGRPGLVIGRKGQGAETIKNDLAKLVKKEVMLEI
                     KEVRRPETDAQIVAEGIASQLERRVAYRRAMKRAVSQALRMGAQGIKVACAGRLAGAE
                     IARSEWYREGRVPLHTLRSDVDYGFKEAFTIYGVIGVKVWIYHGDVIKKQEEASA"
     gene            complement(12508..12840)
                     /gene="rplV"
                     /locus_tag="PLT69_00390"
     CDS             complement(12508..12840)
                     /gene="rplV"
                     /locus_tag="PLT69_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOJ12939.1"
                     /translation="MEARAVLRYSRVSPQKTRLLADEVRNKPIAEALRMLNLLGTKRA
                     RIIAKVLNSAVANALQTGMVDVDNLYVKSIQVDEGPMLKRWMPRAQGRANRILKRTSH
                     IAVVLEEL"
     gene            complement(12844..13125)
                     /gene="rpsS"
                     /locus_tag="PLT69_00395"
     CDS             complement(12844..13125)
                     /gene="rpsS"
                     /locus_tag="PLT69_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOJ12940.1"
                     /translation="MSRSLKKGPFVDDHLLKKVLQAQETGSRKVIKTWSRRSVILPEF
                     VGLTFAVHNGKKFVPVYCTEEMVGHKLGEFSPTRTFYGHAADRKAQKKG"
     gene            complement(13129..>13569)
                     /gene="rplB"
                     /locus_tag="PLT69_00400"
     CDS             complement(13129..>13569)
                     /gene="rplB"
                     /locus_tag="PLT69_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ12941.1"
                     /translation="NVMSLKMIPLGTNVHNVEFRPGKGGQLIRAAGTAGQVLAKEGDY
                     VQIKMPSGEVRKIFAECKATIGQVGNVSHSNITIGKAGRNRWLGIRPTVRGVAMNPVD
                     HPHGGGEGRVKGNHPVTPWGVSTKGHKTRKRTKDSTKYIVKRRK"
     assembly_gap    13570..13579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13580..13946)
                     /gene="rplB"
                     /locus_tag="PLT69_00405"
                     /pseudo
     CDS             complement(13580..13946)
                     /gene="rplB"
                     /locus_tag="PLT69_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            complement(13950..14240)
                     /gene="rplW"
                     /locus_tag="PLT69_00410"
     CDS             complement(13950..14240)
                     /gene="rplW"
                     /locus_tag="PLT69_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOJ12942.1"
                     /translation="MKNATRILKRPLITEKGTDLKEKGNKVMFSVALDANKIEIKKAV
                     EELYGVHVLDVRTMRVKGKSKRWGFRRHQRPDWKKAIVTLGKGETIEFYEGV"
     gene            complement(14237..>14839)
                     /gene="rplD"
                     /locus_tag="PLT69_00415"
     CDS             complement(14237..>14839)
                     /gene="rplD"
                     /locus_tag="PLT69_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOJ12943.1"
                     /translation="NIAGEKVREIQLDDEVFGCEVKPHLLHDVVVWQMANRRAATAKT
                     KGRSEVSGGGRKPWRQKGTGRARAGTNRSPLWRHGGVVFGPNGRRYAMDLPKKVRRQA
                     LRCALSMKAGENMLRVIDSMDLGVPKTKEYVKALGALGMENALVVVGEPTENTSLASR
                     NHPMSKLMQPAGLNVYDVLKYRGLVLDLKAVELIVERLKS"
BASE COUNT         2235 a         4986 c         4259 g         3349 t
ORIGIN      
        1 ttatcgtatg acgatattct ggatgtccta tcgcaggcgg tcgagggtgt caatacacaa
       61 tctatcgggc ctcagccgtc tggggcggcc cttcctctgc ccacgcccct cttcccgaac
      121 tcccccctga acacgctccc ccatgaagat gaacaggcgt ggcatcattc cccatgacga
      181 tctccggccc cgttcatgat gccgatgtac aaccggccct tgtcttgtcg gtcgcgcggg
      241 gcatcgacac cctgcccggc cgcacgcgac ccccgtgaga cacccttggg agatcgtggc
      301 gtgcgccttg tctttcctcg cggagaaacc gtccgccgct tgagcgggcg gcccccatgg
      361 ccgccatctt gctgggcggc ctctggatga gcattgcgtg agacgatgcc gcggcatagg
      421 ccccacatcg gggcaccacc ttgccgagag cgattgtcgt tgacgcgccg gggtcgagct
      481 gccctccgtc aagtcgagga agactccgcc cggcacgtca catgccgcca tggcagggcc
      541 ccgatcacgc ggggcgacat cgacaccgca agcacttgcc tgcagcgtgg gcgcggcgtt
      601 gtgtggggga ccgaccgcgt cggccaccgt gtgccgcgtt cggcctcctg ccgccacgga
      661 gagcgggcaa gagcgccgag gccgctcatg cccccttctt ccgggagcgc ggctgtgcat
      721 tcacggattg gggaggagac ggcctgggga gacctcgcga cggtgaagag ccggcagtgc
      781 gaccggccgc atcaacggca tcgacgggcc tgtctcagcc tctcagcgag tagttcggtc
      841 gccttctcga gatcctcttc ctcgatcccc atgtccgcga ggaaacacct gtagacgatc
      901 tcgtggccgg cctccacccc tgcaccggca agctcggagg ccttgcgggc gagctctatc
      961 gcccctgtgg cgcggtggac gatctttccg ccgaccatga cgagaaccat atcggccgcc
     1021 gagaggtcgt cgagacggtg ggttatgtgg atgacggtcg tcttgagggt cgcgttgagc
     1081 tcacggatct ggtccaagac ctgggacctg cttgcggggt cgagcatggc cgtgccctcg
     1141 tccaggacca gcacgtccac ctccatcgcc agctggccgg catagacgat cttcgagagc
     1201 tgcccgcccg agagttcgga ggtgaggctg tgcctgaggt gctggcagtg caccgcctcg
     1261 agcgatcgcg tgacgcgccg gtctatctcg aaagcgggca gccccaggtt ttcagggccg
     1321 aacgccacgt cctcctccac gacgagcgag acgatctggt tcgccgggtt gccgagcacc
     1381 aggccgaccc gcctgccttt cgtgtcaacc gttccggagg tgggcttcag gacgcccgat
     1441 atgaggtacg cgagggtggt cttgccgctg ccgttgggac cgacgatccc gcaatacgaa
     1501 ccggagggga cctcaaggtc gatcccggcc aaggcgagtg tctggggata cgaaaaggtc
     1561 acgcccgcca acgtgactgc gggcgtgacc tcggcaggtt gcatcaatcc tcggcctctg
     1621 gggccttctc ggccttcttc tccttcttcc tggagaagag gccctttctc gacggctctt
     1681 tcttttcctt gcccggggac ttttccgcgg ccttcttctc ggtccactcg atgatggcca
     1741 tgggtgcgtg gtccccacgc ctcacgctgg tgcggatgat cctggtgtag cccccgttcc
     1801 ggttcgcgaa caggggggcg agctccttga agagcttgcc tatcgccgcc ttcgacctga
     1861 gcacgcgctc cgccctcctg atcgaggcga ggtcgccctt cttgccgagg gtgatcatcc
     1921 agtccgcgag cttcctgagc tccttggcct tcgcctcggt cgtggtgagc ctttcgttca
     1981 tcacgagcga ggtcaccagg ttcctgagca gggcctgcct gtggtcggtg gtgcgctgga
     2041 gtttccttcc tgctatgcga tggtacatgg cgtgtgctcc gatcctactt gctttcttct
     2101 tcttccatcc gcctcctgcg cgcctctatc tcttcggggg acggcaggtt cggcagcttc
     2161 atgccgatgt gaagccccat cttggcgagc ttctccttga tctcctcgag cgacttcctt
     2221 ccgaagttct tggtcttcag cagctcggat tccgacttct ggaccagctc gtagatatag
     2281 gacatgttcg cgttcttcag gcagttggcg gacctgacgg agagctccag ttcctcgacg
     2341 gtcctgaaga ggttgtcgtt gagctcctgc tcgggaccct cctcctcgcc catgtgctcg
     2401 atctcctcct cgtggaagtt gatgaacacg tccatgtatt ccttctggat ccgggcggcg
     2461 taggcgaggg cgtcgtccgg cgtcaccacg ccgttggtcc acacctcgag ggaaagacgg
     2521 tcgtagtccg tcctgcggcc cacgatggtg ggggtcacgt tgaaggacac cctgcgcacg
     2581 ggcgagaaca cggagtccac ggggatccag ccgtggacgt cgagcttttc cttgttgagc
     2641 tccgccggca catagcccct tccccagtcg accctcagct ggagcttgaa ggtgaggtcg
     2701 gtctggatcg tggcgatgag ctggtccggg ttgatcacgt ccatcttccc ggccgtgtcg
     2761 atgtcacctg ccttgacctc gcagggcccc ttcttctcga gggtcaccgt ctggggcttg
     2821 tcggtgtgca tgcgcatcac gaccttcttg aggttcagga tgatgtccgt gacgtcctcg
     2881 tacacgccgg gaatgctcga gaactcgtgg agcacgccca gagactcgtc gaacctcaca
     2941 ccgatgatcg cggaaccgta gatggaggac aggaggatcc tcctcaggga gttgccgatc
     3001 gtgaccccaa agccgcgttc gaggggttgc gcgttgaacc gggcgtaggt gtccgagtac
     3061 gtctcctcca cgatctccag cttggtcggt tttatgatct ctttccagtt tttctctatc
     3121 atccctcacc cgtgcctttg gattcttatt tcgagtagaa ctcgatgatc agagtctcct
     3181 ggatggacgt gggcatgtcc ttgcgctcag gcagggcctt gatgcgcccc ctgcgggctt
     3241 ccttgtccac ctcgagccac tcggcgaaac ccctctgttc gcggttctcg agggaggcgc
     3301 cgatggattc catcttcttg ctcttctcct tgacctcgac cacgtcccca gccttcacga
     3361 ggtacgacgg gatgtccacc ttgcggccgt tgacgaggat gtgcccgtgg ttgatgagct
     3421 gccgcgcggc gttgcgcgag gaagagaagc ccatgcggta gacgatgttg tcgagccgcc
     3481 tctcgaggag ctggaggagc tgttcgccgg tgacgccgcg ggtcctcttg gccatgtcgt
     3541 agtacctgcg gaactgcgac tcggagacgc cgtaggtata cctgaccttc tgcttctcgc
     3601 gcaggtgcag ccggtactcg gtgaccctca ccctgccctg gccgtgctgg cccgggggat
     3661 aggcacgctg ctcgatggcg cacttgtccg tgtaacaccg ctcacccttg aggaagagct
     3721 tctgcccctc cctgcgacag atcttgcaaa cagctcccgt gtaccttgac aagacgtggt
     3781 cctccttctg ttacaccctt ctgcgtttgg gcggcctgca cccgttgtgg gggatcgggg
     3841 tgacgtcgcg gatccccgtg atcctgatac cgctcgcgtt gatcgccctg agcgccgact
     3901 cgcggccgct gcccgggccc tttatgtagg cgaacgcgct cttgagcccg aactcctggg
     3961 ccttcttgca cgcctcctgc gccgccacct gggccgcgaa aggcgtggac ttgcgcgacc
     4021 ccttgaaccc ctgcgcgccg gccgaagacc acgcgagggt gttgccctgc ggatccgtta
     4081 tcgtgatgat ggtgttgttg aaggtggcct ggatgtggac gacaccctca ggcacatcct
     4141 tgcgtaccct cttccgccct gtcctctgtc ttgctttcgc catgaaggat actccttgtc
     4201 tgagacctta cttcttcttg cccaccacga gacggcgcgg ccccttcctg gtcctcgcgt
     4261 tgttgtgggt gttctggccc cgcacgggca gacccttgcg gtgcctgatc ccccggtagg
     4321 acgcgatgtc catgagccgc ctgatgttca tgctgacctc cctgcgcagg tcaccctcga
     4381 ccgtcaggtt cttgtcgatg tagtccctga tggcggcgat gtcgtgctcg gacagttcgt
     4441 cggtcttctt ggcggggtcg accttcacgg ccctcacgat ctcggaggcc ctcgtcctcc
     4501 cgatcccgta gatgtacgta agggcgatgt cgatacgctt gttccgcggt aagtctactc
     4561 cagctatcct agccaagcct tacaacctcc tttacccctg tctctggttg tgccgcgggt
     4621 tctcgcagat cacccgcacg acgccgctgc gcctgatgat cttgcacttc ctgcaccgct
     4681 tcttgattga tgcgctcacc ttcatgtccg cctcgttctc acttggatct gtatacgatc
     4741 cttcccttcg taaggtcgta cggcgaaatc tcgacctgca ccttgtcacc gggcaggatc
     4801 cgtatgtaat gcatcctcat cttgcccgag atatgcgcca gtatctggtg cccgttcgcg
     4861 agctccaccc tgaacatggc gttcgggagg ggctccacca cgatcccttc ggcctcgatg
     4921 acatccttcg ccatgacgtc ttgtgcgcta cgctcctatc gccttctgga tggcttcctg
     4981 cacctgctcc atcgacttcg tgccgtctat cgtcctgacc accccctgct tggagtagta
     5041 gtcgatgagc ggcgacgtct gatcgtgata cgtcttcagt ctgctgcgga tcgtggcttc
     5101 ctggtcgtcg tccctctggt agagctcgcc tccgcacttg tcgcagacac cctctttctt
     5161 cggcgggtcg aactccacgt ggtgcatgta gttgcacccg cggcacgtcc tcctgcccgt
     5221 gagcctcttg accaggtaat cgtccggcac ctcgatgacg accacgtgat cgagactttt
     5281 acccattccc ttgagcagcc cgtcaagcgc cttcgcctgc tccagggtcc tcgggaagcc
     5341 gtcgaggatg aaacccttgg cgcagtcggg ctttccgaag cgctccttca ccacgccgat
     5401 caccacctcg tcgggcacga gcgcgccctt gtccatgtat tccttggcct tgagccccat
     5461 gggggtccct tccttcaccg ccgccctgaa catgtccccc gtggagatct gcggcacgcc
     5521 aagcctgtcg atcatcctct tcgcctgtgt tcccttccct gcgcccggcg gtccaatcaa
     5581 gatgatgttc atgctcattc cccctcggtg agttgtgggt gctaccttct gctgcgtatg
     5641 cgaccggtct tgaggagacc gtcgtactgt ctggtgatga ggtgggactc tatctggctc
     5701 atggtgtcga gagacacgcc gatcacgatg agcagcgacg tgccgccgaa gtagaacggg
     5761 gcgttcgtcc aggagatgag gatcatgggc agcacgcaca cgatgctcag gtagatggcc
     5821 ccgatgaagg tgagccgtga caggaccctg tcgaggtact ccgcggtggc cttgccgggc
     5881 ctgatcccgg ggatgtagcc gccgttcttc ttgaggtttt ccgccacgtc gagcgggttg
     5941 aagatgaccg cggtgtagaa gtaggtgaag aagacgatgg cgatcacgta gatgacgttg
     6001 taccagaacc ccgttggcga gagcgcccct gcgagcgtct tcatgaagcc cacgtccggg
     6061 aagaactgcg cgatggtcgc cgggaacacg atgatcgacg acgcgaagat gggcgggatg
     6121 acgccggaga tgttgatctt gaggggcagg tgcgtgctct ggccgccgta gatcctcctg
     6181 cccttgatct gcttcgggta ctggatgggg atgcggcgct gcgaggtctc gaagtacacg
     6241 atgcccgcga tcaccacggc cacgagcacg accacgagga tgagcgcgat gatcgacatc
     6301 tcgcccgtgc gcacgagcct gaaggtgttg atgatggcga gcggcaacct cgccactatg
     6361 ccggcgaaga tgatgagcga gatcccgttg ccgatgcccc gctccgttat ctgctcgccg
     6421 agccacatga ggaagcacgt gccggtggtc atggtgatca tcgcgaccat cctgaaaccc
     6481 cagcccgggt tgctcaccac cgactggccc gcgacggtca tctgttcgac gccgaaggcc
     6541 atgaagtgac tctgtatgag gcacaacaag acggtgaggt accgcgtgta gaccgtcagc
     6601 ttgtgttttc ccgtcgcgcc ttccttctgg agctgctcga agggcgggta gatcatgcac
     6661 aagagctgca cgatgatgga tgcgctaatg tagggcatca cgcccagtgc gaagatcgac
     6721 aaccccctca ggccgccgcc cgagaacatg tcgaacatcc cgagcagcgt cccctgctgc
     6781 tggtcgaacc actccttcat gatggcggaa tcgatgcccg gcgtgggtat gtgtatgccc
     6841 agcctgtaca cgagcagcat ggcaagggtg aagagaatcc tgttccacag ctcgggaacc
     6901 ttcgctatgt tgctgaaacc gtacccgttg gccacgtgat ggtcctcaga tgtgctcgaa
     6961 ggtgccgccg gccctgctga tcttctctat ggccgcgctg gaggccttgt cgagtctgac
     7021 ggtgagcgga acgtctatct cgccgtcggc caagagtttc accggtaccg acagggaggg
     7081 gatgagaccc cgcatcctga gcgcgtcgcg gtcgaccacg gaacccttct cgaaccgggc
     7141 caggtccttg acgttgacca cggcgtactc gatgcgggtg cggttcttga acccgcgctt
     7201 gggaagtctc cggtaaatgg gcatctggcc gccctcgaac cactggggca cgttcccgcc
     7261 cgagcgagcc ttctgtccct tggtgcctcg gccgcaggtc gtccccatgc cggaggagtt
     7321 gccccggccc acgcgcttct tcttcttcct cgacccgtcg gccggcttga ggttggatag
     7381 atcgatcatc tcacgcctcc tcccatttca ccaggtgggg aacctttgcg accgcgcccc
     7441 gcgtcgcggg gttgtccggc agcaccctcg acctgccgcg cctcttgagg ccgagcgcgg
     7501 cgacgatcgc cttctgcttc tctgtacaac cgatcaccga ccgatacagc gtcaccttga
     7561 tcttgcctgc catggtctcc acccgttcct tgctaccttc acgaacccag atccagcgtc
     7621 ttgccgcgct tggcctcgac gtactccctc gtctccagac gatcgagcgc gtcgaaggtg
     7681 gcccgtatga cgttgttgtg gttgcgcgat cccagcgtct tggccaccac gttccggtac
     7741 cctgcggcct ccatcacggc cctcaccgcg ttcccggcga tgataccggt gccttccggc
     7801 gcaggcttca cgatcaccct ggaagagccg aactccccga tgatctcgtg cgggaccgtg
     7861 cccgagacga tggggacgtc cctcatggcc ttcctggcgg actcgtatcc cttgcggatg
     7921 ctcacggata cttccttggc cttgcccagc ccgtgaccga ccttcccgtt gccgtcgccc
     7981 accaccacga gtgcgctgaa acggaacctc tttccgccct tgacgacctt cgcgacacgg
     8041 ttgatgtaga cgaccttgtc gatcaactgt tcctggttct cgtcgatctg ctcgaatcta
     8101 gccaatggtg cacctcgctt gtctagaact tcagcccacc ctcgcgggcc gcctcggcca
     8161 ccgccttgac gcggccgtgg taccggtacc ctgagcggtc gaacacgacc ttgtcgatcc
     8221 ccttgtccct ggccttggcg gcgagcagct cgcccacgag ccttgccgcc gcgatgttcg
     8281 cccccttgac cttccccttg atctcggggc tcaaggtgga ggcggcggcc agggtcacac
     8341 cgttctcatc gtcgatgatc tgggtataga tatgcctcag accgcggtac acgctcaggc
     8401 gcggcctgtc ggccgtgccc gtgatcctct tgcgaatcct ttccttcctg agctgccacg
     8461 cctgccgttc tgtcttcttg ctcacttcac gccactcctt gtctcactgc atgggcatca
     8521 gcccttggcg gccgtcttgc cgggcttgag cctgacctgc tccccgtcat agcggatacc
     8581 cttgcccgcg tacgagtcgg gcttgcggat cttgcggatg cgcgctgcga cgtcgccgag
     8641 gagctcgttg tctatgccgg tgatctcgat gaggccgtcc ttgctcaccg tgccgctcac
     8701 cccctcgggc aggcggaact cgacgggatg ggaatacccc acgttcaaca ccagggtgtt
     8761 gcccttggcc tctccacggt acccgatgcc gacgatcctg agcctcctgg tgaagccctc
     8821 ggagaccccc ttcaccatgt tgttcgcgag cgccctcatg agcccgtgga atgccttcgt
     8881 cttgcgctcc tcgtccttcg ggacgaagcg gagttccttc tcctctatca ccacgtccag
     8941 attcccgacg caacgcttct tgagctcgcc cttgggcccc ttgaccgtga gcgtctcgcc
     9001 gtcgaaggtg acgctcaccc ccttgggaat gggtataggc agttttccta ttcttgacat
     9061 cgccatggtc agctcctcac caaaccctca gcaggacttc gccgcccttg ttcttggccc
     9121 tggcattgtg gtcggtcatg atgccttcag acgtgctcac gatggctatg ccgagcccgc
     9181 cccggacctt ctctatctcg gccgccccac ggtaaatcct cctgcccggt ttcgagacgc
     9241 gctcgatgcc gctgatcacg tggcgggagt tgaagtactt gaggctcacg cggagcatcc
     9301 cctgcctgcc gtcgtcgatc acgcggtagg actttatgta accctctttc tggaggatgt
     9361 ccgcgatcgc tatcttcatc ttcgacgcag ggatgtcgac gctctccttc tgggcccctg
     9421 cagcgttccg gatcctggtt atcatgtccg ctatgggatc ggtgtgcatc tgcgttcccc
     9481 tcttaccagc tggctttctt cacgcccggg atctcgcccc tcatggcatg gttcctgaga
     9541 catatcctgc agatcccgaa cttgcggtag tacccgtgcg gcctgccgca aagctggcac
     9601 ctgttgtact gcctcacctt gaacttgggc ttcctgcgct gcttagcgat catcgatttc
     9661 ttcgccatga catcccctca tctacttccg aaacggcatt ccgaaggcgg caagcaggct
     9721 cctgccctcg tcgtcgctgc cggcagtggt gacaatggtt atattcatgc ccctgagctt
     9781 gtcaatcgcg tcatagttga tctcggggaa gaccgactgg tcgttcaggc ccatggtgta
     9841 gttgcccctt ccgtcgaagc ccttggggga gacgcccctg aagtccctca cacggggaag
     9901 caccgcgttg atgagcctgt ccaggaactc gtacatgcgc tcacccctga gcgtcaccga
     9961 gcagcccacc ggcatgccct ccctgagctt gaacgcggcg atggacttgc gggccttgcg
    10021 taccaccggc ttctgcccgg tgataagggc cagttcctcg gcggcccggt cgatgatctt
    10081 gatgtcctgc accgcatcgc cgagccccat gttcaggacg atcttgacga gcttcggcac
    10141 ctggtggacg ttcttgtacc cgtactccgt cctgagggcg ggaacgatct ccttgaggta
    10201 gtattccttg agccttgcca tctcacatcc tcacttgtcc agttcctcgc tgcagctcct
    10261 gcagtacctg accttggtcc cgtcctcgtg gaacttgaag cctatgcgga ccgccttctt
    10321 gcaagcgggg cagacgatgg ccacgttgga gaggtggatc ggcgcctcct tctccacgat
    10381 cccgccctgt gacttctgcg acggcctcgt gtgccgcttg atcatgttga cccgctcgac
    10441 gacgacgcgg tccttcttgg ggacgacacg gatcacctga ccctgcttcc ccttgttctt
    10501 cccggtgagg acgatcaccg tgtctccctt tcttatcctg ccggtctcat gccttaccac
    10561 tgccatcgcg cacctctaga gaacctctgg agccagggac acgatcttca tgaagttctt
    10621 gcttctcagc tccctggcca ccggcccgaa gatacgagtc ccgaccggct cgttctggtt
    10681 gttgaggatg acggccgagt tctcgtcgaa cctgatgctc gtcccgtccg gcctcttgtg
    10741 cgtcctcgtc gtgcggacca ccacggccct caccacgtca cccttcttca ccttggacct
    10801 cgggatcgcg tccttcacgc tcaccacgat gatgtccccg agggaggcgt acctgcgcct
    10861 ggtgcccccc aggaccttga tgcacatgac ctccttggct cccgagttgt ccgcaacctt
    10921 gagtcttgtc tgctgttgga tcatgatcgg cctccgccac ctgcacctct accggctact
    10981 cctgtctctc gaggatcttc acaaccctcc agcacttctc tctgctgatg ggcctgcatt
    11041 cgcggatcag caccctgtcc ccgggtctgc actcgttgcg ttcgtcgtgc gccttgaact
    11101 tcgcacgcct gcgcacgtac ttgccgaaca ccgggtgttt ctcgaggcgc tcgaccgtga
    11161 ccaccacggt cttgtccatc ttgtcgctcg tgacgattcc ttcgaattca agcttcctgg
    11221 cctgttccgt cattgcactc tctcccgcgc gatcgtattg atacgtgcga tgtctcgacg
    11281 cagcttggag atgatcgacg tgtccttgag ctggccggtc gccttcttga accgggcgtc
    11341 gaggagttcc ctctccagct tcaagaggag ctccgagagc tcggcagggc tcttctcgcg
    11401 tatctccttg gccttcatca cacaccctcc ctgaccacga acctcgtttt cacgggcagc
    11461 ttgtgggaag ccaggcggaa cgcctccttt gcctgttcct cggtcacgcc gtcgatctcg
    11521 aagaggatcc tgccgggtct caccggtgcg acccactctt cgggagcgcc cttcccctta
    11581 cccatgcggg tctccgccgg cttcttggtg atgggcttgt ccgggaagac ccgtatccag
    11641 accttccctc cgcgcttgat ggaccgggtg atggcgatac gggccgactc tatctgcctc
    11701 gcggttatcc agtggggttc gaggctctga aggccgtacg tcccgaagga gacgcgcgag
    11761 ccgcgttccg ggctgcccgt gagcctgccc cgctgctgtt tcctgtgctt gaccttgttc
    11821 ggcatcaaca tgactcatgc actccttacg cgctcgcctc ttcctgcttc ttgatgacat
    11881 cgccgtgata gatccagacc ttcacgccga tgaccccgta gatggtgaag gcctccttga
    11941 acccgtagtc cacatcgctc ctgagcgtgt ggagcggcac gcgaccctcg cggtaccatt
    12001 cgctcctggc gatctcggcg ccggcgagcc tccccgcgca ggcgaccttg atgccctgcg
    12061 cgcccatcct gagcgcctgg ctcacggccc gtttcatggc cctcctgtag gccacgcggc
    12121 gctcgagctg gctggcgatc ccctccgcca cgatctgagc atcggtctcc ggccgcctca
    12181 cttccttgat ctcgagcatc acttccttct tcacgagctt ggcgaggtcg ttcttgatgg
    12241 tctccgcgcc ctgccccttc ctgcctatca cgagacccgg cctgcccgtc gagatcacga
    12301 gcttcacctt gcccgccgca cgctcgatga tgatgttgga gatccccgcg tgactcaggt
    12361 tcttggtgat gaagtccctg atcttgcgat cctcgacgag gagctcgccg aagcccttgt
    12421 cggcgtacca cttcgccttc cagtccttgg tcacgcccag ccggaacccg tatggatgaa
    12481 tcttctgtcc caccttcgac ccctttccta gagctcttcg aggaccaccg ctatgtggct
    12541 ggtccgcttc aggatgcggt tggcgcggcc ctgtgccctc ggcatccacc tcttcagcat
    12601 gggaccctcg tcgacctgga tggacttcac gtagaggttg tccacgtcga ccatgcccgt
    12661 ctggagggcg ttggcaaccg cggaattgag cacctttgcg atgatgcgcg cacgcttcgt
    12721 gccgagcagg ttgagcatcc tgagcgcttc cgcgatgggc ttgtttctca cctcgtcggc
    12781 gagcagtctc gtcttttggg gcgagaccct cgagtatctg agaacggctc gtgcttccat
    12841 gggctacccc ttcttctgcg ccttcctgtc ggccgcgtgg ccgtagaacg tgcgtgtggg
    12901 agaaaactcc ccgagcttgt gccccaccat ctcctcggtg cagtacaccg ggacgaactt
    12961 cttgccgttg tggacggcga aggtcagccc cacgaattcc gggagaatga ccgagcgcct
    13021 cgaccacgtc tttatgacct tgcggctccc tgtctcctgg gcctgcagga ccttcttcag
    13081 gaggtggtca tccacgaacg ggcctttctt cagtgaacgg gacacgtctt acttcctcct
    13141 cttcacgatg tacttggtgg agtccttggt ccgcttgcgg gtcttgtgtc ccttggtgga
    13201 gaccccccaa ggcgtcacgg ggtggtttcc cttgacgcgg ccttctcctc caccgtgggg
    13261 atgatcgacg gggttcatgg ccacaccgcg caccgtcggc cgaatgccga gccagcggtt
    13321 ccttccggcc ttcccgatgg taatgttgga atggctgacg ttgcccacct ggccgatggt
    13381 cgccttgcac tccgcgaaga tcttcctcac ctcgcccgag ggcatcttga tctggacgta
    13441 gtcgccctct ttggcgagca cctgccccgc cgtgcccgcc gccctgatga gctgtccgcc
    13501 cttgccgggc ctgaactcca cgttgtggac gttcgtgccg agggggatca tcttgaggct
    13561 catgacgttn nnnnnnnnnc cgcctcctcc gaggtgatga ccgtgctgcc cacggccagc
    13621 ccgtcaggcc agaggatgta ccgtttctcg ccgtccgcat actggacgag ggagatcctc
    13681 gccgagcggt tggggtcgta ctccacggtc atcaccttgc cggggacccc caccttgtcg
    13741 cgcttgaagt cgatgatgcg gtacagcctc cgactccccc caccctggtg gcgcacggtg
    13801 atcctgccga ggttgttcct cccgctgtgc ttcttgaggg tgacggtcaa ggaccgctcg
    13861 ggttcctgcg ccgtgatctc ctcgaaggtc gagacggtca tgccgcgcct tccggcggac
    13921 gtcggtttga agctacgaat gctcatcggt catacccctt catagaactc gatggtctcg
    13981 cccttcccga gcgtgacgat cgccttcttc cagtcgggcc gctggtgtct cctgaagccc
    14041 cacctcttgg acttgccctt gaccctcatg gtcctcacgt cgagaacgtg cacgccgtag
    14101 agctcttcca cggccttctt gatctcgatc ttgttggcgt cgagagccac ggagaacatc
    14161 accttgttcc ccttctcctt gagatccgtg cccttttccg tgatcaaagg gcgcttgagt
    14221 atgcgggtcg cgttcttcat gacttcagcc tctcaacgat gagctccacg gccttgaggt
    14281 cgagcaccag tcccctgtac ttcagcacgt cgtagacgtt caggcccgcc ggctgcatga
    14341 gtttgctcat ggggtggttc cgggaggcga gagaggtgtt ctccgtcggc tcgcccacga
    14401 ccacgagggc gttttccatg ccgagggcgc ccagggcctt cacgtactcc ttcgtcttgg
    14461 gaaccccgag atccatgctg tctatcaccc tcagcatgtt ctcgccggcc ttcatgctca
    14521 gcgcgcacct gagcgcctgc cgccttacct tcttggggag atccatggcg tagcgcctgc
    14581 cgttcgggcc gaagaccaca ccaccgtgcc tccacagcgg cgaacggttc gtaccggcac
    14641 gggcgcgtcc cgtccccttc tgtctccacg gtttcctgcc gccgcccgag acctcggagc
    14701 ggcccttcgt cttggcggtc gccgcccggc ggttcgccat ctgccagacg accacgtcgt
    14761 ggaggagatg cggcttcacc tcgcagccga agacctcgtc gtcgagctga atctccctga
    14821 ccttctcccc agctatgtt
//
LOCUS       DAOSCR010000005        12757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_3313_length_12757_cov_3.578413, whole genome shotgun sequence.
ACCESSION   DAOSCR010000005 DAOSCR010000000
VERSION     DAOSCR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 12757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12757
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_12757_cov_3.578413"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1127
                     /gene="tsaD"
                     /locus_tag="PLT69_00420"
     CDS             48..1127
                     /gene="tsaD"
                     /locus_tag="PLT69_00420"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOJ12944.1"
                     /translation="MNPPPYILGIETSCDETAAAVVTGGGRVLSNVVASQVKDHEAFG
                     GVVPEIASRRHVELIEPVVKSALAQAGVTLSDIGAVAVTQGPGLVGALLVGISYAKAL
                     AYGLGVPVCGVNHLEGHLAAAKIEHELTYPHVGMVVSGGHTSLYRVDDEDTIELVGQT
                     QDDAAGEAFDKVAKLLHLGYPGGVVIERIAKGVDPGAFELPRPMISDASFDFSFSGLK
                     TAVLHLVQKEGIFDSADLGFSGLPEPRKTAPGKESLIPLIAAAFQAAVVDVLATKATR
                     AVKAFGMDTLVVSGGVASNTALREELRARCADEGVRLVIPSRAYCTDNAAMIGVAGLH
                     HLARGRTAGLDMNALSRWDIAASRR"
     gene            1157..1969
                     /gene="rsmA"
                     /locus_tag="PLT69_00425"
     CDS             1157..1969
                     /gene="rsmA"
                     /locus_tag="PLT69_00425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ12945.1"
                     /translation="MRPKRSLGQNFLTNAGIARKMAELACIGPGDTVVEVGPGRGMLT
                     RCLLSSAGRVLAVEKDDGLVDHLRHVFSGEDRLVLVHGDILECDLHELVPQGAKIVAN
                     LPYNIASRFVLRLTEAAGAIDRAVLMLQKEVADRLCAKPSDTAYSALTVIVSASFSTS
                     PGFTVGPANFFPRPKVDSRVVVLTPRSPAGDMPDPGIFARVVRSAFSRRRKVLANTLV
                     HLEGMDARSLSSIAGACSVDLRSRPQDVSVDAYLSFCREYEAFLGQGRTLHR"
     gene            2345..3712
                     /locus_tag="PLT69_00430"
     CDS             2345..3712
                     /locus_tag="PLT69_00430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOJ12946.1"
                     /translation="MKGTRIVTASAIVLLLLASALHASSYRTDRIIVKFRQAVPGTAS
                     AVAALSSRPVAIAVDDAQKAIAELEASGEVEYAEPDYIIEAEEVPSDWPYRGQWAQLA
                     IEQAWDLFSSSGGSSSVIVAVVDSGVDFSHPDLSGVLVKGYDFVAKDDVPEDNSGHGT
                     KVCGVLGAVGGNGIGIAGVAWEVDLAVMPLKFMDTDSSGKTTGNLSDAIKAIYYAVDH
                     GARIINASWGFYDYSRSLEEAIEYARSKGVLVVCSAGNKGQDNDVLDHYPSNYPQDNI
                     VAVAALGTDGTLAAFSNYGVKNVDVAAPGVGLVTTTLNGGYASWVSGTSYSTPFVSGV
                     AAMLLATDDTISPEEARDILIRSCSQSPQAASFAIAGLGCINAFEALALAGSHDETSS
                     QDSGGEPSRTAEGSSDQGMDDGGSSGGSGCLISTAAPSPSIGMLLPVLLTIVLFRTRR
                     TGLPE"
     gene            complement(3872..5161)
                     /locus_tag="PLT69_00435"
     CDS             complement(3872..5161)
                     /locus_tag="PLT69_00435"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOJ12947.1"
                     /translation="MAEVVIIGGARTAVGAFGGSLKDVPAKDLGALVIRETLLRLGLA
                     PEVSAPAKADGPDTFKGAPLSEIEGKYANWPEGSMKIMVDEVIMGNVLMSGQGQNPAR
                     QAMIRAGLPKETPAFTINKVCASGMKAIALGAQSIKAGENEVVIAGGMENMSDVPIAL
                     PKARWGYRMDMPYGQTVDLMVFDGLYEIFYGYHMGVTAENIAQKYGITREEQDRLGVD
                     SHARARAAIASGILKGEIVPVKIPQKKGDPLVFDVDERPMDTNLEKMSKLPTVFKKDG
                     TVTAGNASGINDAAAALLLMSEAKAKDLGLKPIARIKGYAAGGVDPSYMGLGPIPAVR
                     KLLRITGTTIKDYGLFELNEAFASQAIACMRELGMDWSITNVNGSGISIGHPIGCTGA
                     RIVLALINEMQRRNVSLGLATLCIGGGMGMALAVEAV"
     gene            5326..6204
                     /locus_tag="PLT69_00440"
     CDS             5326..6204
                     /locus_tag="PLT69_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ12948.1"
                     /translation="MELDYRAILNRYAVSLMEGRRLSPETIRAYVKDLEDLVAFLGEH
                     GFPVPDRRLVRSYLLRLTSRYDRSSVNRKLSAARGFFGYVAKVHGLGANPFGHVRSLK
                     RARSLPRFLSPDEIFDLIEKVSDPRDRALLELLYSTGIRVGEAEGLKCCDVDMQEGFV
                     LVTGKGSKQRMVPVGSRAIEAVRTYLLTRGIEDPLYCTEPLFLGRRGGRLCSRVMRRI
                     VEGWAMQAAIAKRVSPHVIRHTFASHMLDAGADLRSIQEMLGHASLSTTQRYTHLHVD
                     KLMEVYDKAHPRAREG"
     gene            6204..6737
                     /gene="hslV"
                     /locus_tag="PLT69_00445"
     CDS             6204..6737
                     /gene="hslV"
                     /locus_tag="PLT69_00445"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOJ12949.1"
                     /translation="MDIHATTILCVRRFGRVVMAGDGQVTMSSVVLKATAKKVRKLYE
                     GKVLAGFAGATSDAFTLFERFEDKLKEFRGNLTRAAVEMGKDWRTDRVLRRLEALMIV
                     ADPSKTFLLSGMGDVVEPDDEVCGIGSGGHYAMAAAKALIRHTDLDAPSIAKISMEIA
                     SEICIYTNREIHLEELP"
     gene            6734..8044
                     /gene="hslU"
                     /locus_tag="PLT69_00450"
     CDS             6734..8044
                     /gene="hslU"
                     /locus_tag="PLT69_00450"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOJ12950.1"
                     /translation="MKDLTPRQIVSELDRYIVGQDRAKRAVAIALRNRWRRQNVPEEM
                     RDEIYPKNIILIGPTGVGKTEIARRLARLAHAPFIKVEASKFTEVGYVGRDVESIIRD
                     LMRTGVQLVQAEESEKVRAKAVEAAKERVLDHLLPAQKDTEIHAESREKLKRMLEEGV
                     LDDRFVEVEFRETQMPTIEVFSGQGLEEMGIDFKEMFSSMLPKRTKRRRMSVKEALEF
                     LTNQEIARLVDMDKVTQEARRRVENTGIVFIDELDKIAGRESGRGPDVSREGVQRDLL
                     PIVEGTTVNTKYGPVNTDHILFIGAGAFHVSKPADLIPELQGRFPIREELGPLTKDDF
                     VRILTEPKNALTRQYQALLATEGIELTFTEEAIAQIAAIAQHINDQTENIGARRLHTV
                     MEKLLEEVSFAAGERAGPVNIDGDYVRERLEPLMAREDLSRYIL"
     gene            8214..9191
                     /locus_tag="PLT69_00455"
     CDS             8214..9191
                     /locus_tag="PLT69_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ12951.1"
                     /translation="MIPKGKFVSEIQDGDRVKAVFLLADRRLLTARNGKPYGRVVVSD
                     KTGDITGLIWDDAPEQISAVSPGHVVGVRATAESYDRKVQLRIEKITRLADKEVDLSA
                     LIPTSPRDLPALEALLDEAIDGMKDPFLKALVNRVFARPGVRESFVKAPAAKGIHHIG
                     GLLEHTVTVIRAADALLPVFAHLELNRDMIIAGAILHDLGKIYEYTYGKVIDMTPVGR
                     LLGHIYLSAHMVDEEACAIEGFPEELRLQILHMILGHHGQLEFGSPKLPMTREAVFLH
                     MMDDLDAKLTGITSLIQATPEDEPFTAFSSVYNRFLYTRTYRDDPGEEK"
     gene            complement(9687..9929)
                     /locus_tag="PLT69_00460"
     CDS             complement(9687..9929)
                     /locus_tag="PLT69_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ12952.1"
                     /translation="MKTKTLNHIILMSAWSFTLVVSSFLFLFVGRWIDVRLGTEPAFM
                     LGLFVLAVGLCVGRIYTDYIRTKDELNIRRVRPHHL"
     gene            complement(10037..10312)
                     /locus_tag="PLT69_00465"
     CDS             complement(10037..10312)
                     /locus_tag="PLT69_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12953.1"
                     /translation="MKRTVQTVVVALFVVLITSTFAFAAAATAGGSNFPYFHLGCLIM
                     GGLTIVSLKHRYPRIYMSEAVGSFALYAVLVALFTAPVIEAVKTIVS"
     gene            10542..11237
                     /locus_tag="PLT69_00470"
     CDS             10542..11237
                     /locus_tag="PLT69_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOJ12954.1"
                     /translation="MLQSGYPAERCGLVKVRPGKRHVRRGRPSDETDGQDERESQTPP
                     KVLVLLGSPRRKGNSTLLAQRIAKGAEKAGASVEYVYLHGLDIRACTGCMKCQRKGAK
                     GCVIKDDMQDLYRLMKECDAWVVASPVYWFTVSAQLKLWMDRCLAFTSYTPDAFSGKR
                     IAIAMTYGGEDPFDSGCINALRTFQDAYSYVGARIVGMVYGSAMAEGEIAKDAGVLSK
                     AEDLGHLLVAADG"
     gene            complement(11689..11967)
                     /locus_tag="PLT69_00475"
     CDS             complement(11689..11967)
                     /locus_tag="PLT69_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12955.1"
                     /translation="MDYLVVRCAFCTRRYLVEIEGIDAWWASCPCGAHGFAEDDGGLG
                     VDADSRPGFTVKEVSSGLWGEALLSDPAVVYVDEWARRWYVFWVLPQG"
     gene            12072..>12757
                     /locus_tag="PLT69_00480"
     CDS             12072..>12757
                     /locus_tag="PLT69_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ12956.1"
                     /translation="MARVRFEDTSCGIDAAVTKALDSFGSPRSLLRRSGAVFLKVNLI
                     DSKPHVFTSVEVVRAIVTRFLAGGARAVYVIENSTQGNFTRLVFEATGMRRMCEETGA
                     IPVYLDETAEMPMYLERLGSFVRIPDFVHEHLVRKRDENLYISVPKLKTHSMSTVTLG
                     IKNQFGLLHHRSRIPDHNFRLHQKIADIYANIRPDLTLIDATEATNHGHYPATGFASE
                     CVVPLDLVIAG"
BASE COUNT         2355 a         4250 c         4067 g         2085 t
ORIGIN      
        1 aaggacgagc cttgagccgc atagctggac gcccgcgcga gcgcgagatg aacccgcccc
       61 cctacatcct cggcatcgag acctcctgcg acgagacggc cgccgccgtg gtcacgggag
      121 gcggccgagt cctctccaac gtggtggcga gccaggtgaa ggaccacgag gccttcggcg
      181 gggtcgtgcc ggagatcgcg tcccgcagac acgtggagct catagaaccg gtggtgaaga
      241 gcgcgctcgc tcaggcgggc gtcacgctgt cggacatcgg cgccgtcgcc gtcacccagg
      301 gaccgggtct cgtgggagcc ctgctcgtgg gcatctcgta cgccaaggcg ctcgcgtacg
      361 ggctcggagt gcctgtctgc ggggtcaacc acctcgaggg ccaccttgcg gcggcaaaga
      421 tcgagcacga gctcacctac ccgcacgtgg gcatggtggt ctctggcggc cacacctcac
      481 tctacagggt cgatgacgag gacaccatcg aactcgtggg ccagacccag gacgacgccg
      541 cgggcgaggc cttcgacaag gtcgccaagc tcctccacct gggctatccc ggcggcgtgg
      601 tcatcgagcg gatcgccaag ggggtggacc ctggggcctt cgagttgccg aggcccatga
      661 tcagcgacgc gtccttcgac ttcagcttct ccggcctcaa gaccgcggtg ctccacctcg
      721 tgcagaaaga ggggatattc gactccgcag acctcggctt ctcgggcctc ccggaaccga
      781 gaaagaccgc gccgggcaag gagagcctga tccccctcat cgccgccgcg ttccaggcgg
      841 ctgtggtgga cgtgctcgca acgaaggcca cccgcgccgt gaaggccttc ggcatggaca
      901 cgctcgtggt ctcgggcggc gtggcgagca acacggcgct cagggaggag ctccgggcgc
      961 gatgcgccga cgaaggggtc aggctcgtca tcccgagccg ggcctactgc acggacaacg
     1021 ccgccatgat cggggtagca gggcttcacc acctcgccag gggcaggacc gcggggctgg
     1081 acatgaacgc cctgtcgagg tgggacatcg ctgcatccag gcggtgaccc cccgcgtgga
     1141 ggcgcgcgca cgtggaatga gaccgaagcg ctccctcggc cagaactttc tcacgaacgc
     1201 gggcatcgcc cgcaagatgg ccgagctcgc ctgcataggc cccggtgaca cggtggtcga
     1261 ggtcggcccg ggcaggggca tgctcacccg ctgcctgctc tcctctgcgg gaagggtcct
     1321 ggccgtcgag aaggacgacg gactggtcga tcacctaagg cacgtctttt cgggcgaaga
     1381 caggctcgtg cttgtccacg gggatatcct cgagtgcgac ctccatgagc tcgtcccaca
     1441 gggcgccaag atcgtcgcca acctgcctta caacatcgca tcccggttcg tcctgaggct
     1501 gaccgaggcg gcgggcgcaa tcgacagggc ggtgctcatg ctccagaagg aagtcgcgga
     1561 tcgcctgtgc gcgaaacctt ccgacacggc gtactccgcc ctgaccgtga tcgtctccgc
     1621 ctcgttcagc acatctcccg gattcacggt cgggcctgca aacttcttcc cgaggccgaa
     1681 ggtggattcc cgggtcgtgg tcctcacccc gaggtctccc gccggggaca tgccggatcc
     1741 gggtatcttc gcgagggtgg tgaggtccgc gttcagccgg aggcgcaagg tgctcgcaaa
     1801 cacccttgtc cacctcgagg gcatggatgc ccgctccctc tcctccatcg ccggggcgtg
     1861 ctccgtggat ctcaggtcac ggccccagga cgtttccgtg gatgcgtacc tgtccttctg
     1921 cagggagtac gaggccttcc tcggccaggg ccgcaccctg catcggtagc acccccatca
     1981 tccttcggcg cgccaggcaa gggaaccggg tgacaacccg ttcgacagcg gggcaagcct
     2041 cggggcgccc ctgcgtggag acctgcacag aggccccgcc ccatcaggcg cacccgaggg
     2101 cgacgatcca acccatgcgc gccgggcacc acgccggtcg acagttacac cggttcgatc
     2161 cgagccctgt aatccggatt acagggctga aacgcttgta accgtcaaca tcgccgtcat
     2221 tttcctctcg acatgatcta tctatttatt atttctgtgc tttttgattg tgctctcatc
     2281 gccaaggatt tggcacccgg cttgccttgt ccctgtctga tcattcgatc atggggggta
     2341 acaggtgaaa gggacacgga tcgtcactgc atcggccatt gtcttgctgc tcctggcaag
     2401 cgcgctccac gcatcttcct accggacgga ccggatcatc gtgaagttca ggcaggccgt
     2461 ccccggcacg gcatcggcag ttgccgccct ctcgtcgagg cccgtcgcga tcgcggtgga
     2521 cgacgcccaa aaggccatcg ccgagctcga ggcatcgggg gaggtggaat acgccgaacc
     2581 cgactacatc atcgaggcgg aagaggtccc ctcggactgg ccgtaccggg gacagtgggc
     2641 tcagctggcg atcgagcagg cgtgggacct gttctcatct tcgggaggct cgtcgagcgt
     2701 catcgtggcg gtcgtcgact ccggtgtgga cttctcccac ccggacctct ccggggtcct
     2761 cgtcaagggg tatgacttcg tcgcgaaaga cgacgtcccc gaggacaact cgggtcacgg
     2821 caccaaggtg tgcggcgtcc tcggggcggt cggcggaaac ggcatcggca ttgcgggggt
     2881 ggcctgggaa gtggaccttg ccgtcatgcc cctgaagttc atggacaccg acagctcggg
     2941 caagaccaca ggcaacctct ccgatgcgat caaggccatc tactacgcgg tggaccacgg
     3001 cgcccggatc atcaacgcga gctggggctt ctacgactac tccaggtcgc tcgaggaagc
     3061 catcgagtat gcgcgaagca agggcgtgct cgtcgtgtgc tccgcgggga acaagggcca
     3121 ggacaacgat gtcctcgacc actacccttc caactatccg caggacaaca tcgtcgccgt
     3181 ggcagcactg gggacggacg gcacgcttgc cgccttctcg aactacggcg tcaagaacgt
     3241 cgacgtggcc gctcccgggg ttggcctcgt caccaccacg ctcaacggcg gctacgcctc
     3301 ctgggtctcg ggcacgtcgt actccacacc cttcgtgagc ggcgtggcgg cgatgctcct
     3361 ggccacggac gacaccatct cgccggaaga ggccagggac atcctcatcc ggtcctgctc
     3421 ccaaagcccg caggcggcgt ctttcgccat agccgggctc ggctgcataa acgccttcga
     3481 ggccctggcc cttgcaggca gccatgacga gacctcctcc caggattcgg gcggcgaacc
     3541 ctcgaggacg gcggagggct catcggacca gggcatggat gacggcggct cgtcaggcgg
     3601 ctcagggtgc ctgatctcga ccgcggcgcc ttcgccgtcg ataggcatgc tcctcccggt
     3661 cctcctcacg atagtcctgt tccggaccag gcgcacaggc ctgccggaat agcctatacc
     3721 ccccagcccc ccgccctgcg gggggcctcc ccaaagagaa cgggggccgc gctcaaaacg
     3781 cggcccccgt ggctttctcc ggcccgaagg cagcctcgta ccaccttccc cgggcttatc
     3841 cttcgagcat gtatcaagcc ccaagccttc cctagaccgc ctccacggcg agcgccatgc
     3901 ccatgccgcc tccgatgcac agggtggcca gcccgaggga gacgttcctc ctttgcatct
     3961 cgtttatgag cgcaagcacg atgcgcgcac ccgtacagcc gatgggatgg ccgatggaga
     4021 tgcccgagcc gttcacgttg gtgatcgacc agtccatgcc gagctcccgc atgcaggcta
     4081 tcgcctggga ggcgaaggcc tcgttgagct cgaagaggcc gtagtccttg atcgtggtgc
     4141 cggtgatgcg caggagcttg cgcacggccg gtatcgggcc gaggcccatg taggacgggt
     4201 cgaccccgcc cgcggcatac cccttgatcc gggcgatggg cttcaggccc aagtccttgg
     4261 ccttggcctc gctcatcaac aggagggcgg ctgccgcgtc gttgatcccg gaggcgttac
     4321 ctgccgtgac cgttccgtcc ttcttgaaca ccgtggggag cttggacatc ttctcgaggt
     4381 tcgtgtccat gggccgctcg tccacatcga acaccagcgg gtcgcccttc ttctggggga
     4441 tcttcaccgg cacgatctcg cccttcaaga tgcccgaggc gatggcggcc ctcgcccgcg
     4501 cgtggctgtc gacccccagc ctgtcctgct cctcgcgggt gatcccgtac ttctgcgcta
     4561 tgttctccgc cgtgacaccc atgtggtacc cgtagaagat ctcgtagagg ccgtcgaaga
     4621 ccatgaggtc gacggtctgg ccgtagggca tgtccatgcg gtagccccac ctggccttgg
     4681 gcagggcgat ggggacgtcg ctcatgttct ccatgccgcc cgctatcacc acctcgttct
     4741 cgcccgcctt gatggactgt gcaccgagcg caatggcctt catgcccgag gcgcacacct
     4801 tgttgatggt gaaggccggc gtctccttcg ggaggcctgc cctgatcatc gcctgacgtg
     4861 cagggttctg gccctggccg gacatgagga cgttgcccat gatgacctcg tccaccatga
     4921 tcttcatcga gccttccggc cagttcgcgt acttcccctc gatctcgctt aagggcgctc
     4981 ccttgaaggt gtccggcccg tcggccttgg caggcgccga gacctcgggc gcgagcccca
     5041 gcctgaggag ggtctcgcga atgaccagcg cacccagatc ctttgcaggc acatccttga
     5101 gcgaaccacc gaaagcgccg accgcagtcc tcgcgccgcc tatgatcacc acttcagcca
     5161 tagagaggtt cctcctgaag attcgtttgt ccttccgtct cggccgatcc ggccgcctgg
     5221 agactatacc atcgcgcccg gcttttggga accttcacct tggggcgcac ccggaaaaga
     5281 cgaccttcct tgattcatgc acctcgaggg tgtacaaccc ctcgcatgga actcgactac
     5341 cgggccatcc tcaaccgcta cgccgtctcc ctcatggagg ggaggaggct ctcgccggag
     5401 acgatcaggg catacgtgaa agacctcgag gacctggtgg ccttcctcgg cgagcacggt
     5461 ttcccggtgc cggaccggag gctcgtccgc tcctacctgt tgaggctcac gtcccggtac
     5521 gaccgctcgt cggtcaaccg caagctgagc gcggcgcggg ggttcttcgg gtacgtggcc
     5581 aaagtccacg gcctcggcgc caacccgttc ggccacgtga ggtccctcaa gcgggccagg
     5641 tccctgccca ggttcctgtc ccccgacgag atcttcgatc tcatcgagaa ggtgtccgac
     5701 ccccgcgacc gcgccctcct cgagctcctc tactcaaccg gtatccgcgt cggcgaggcg
     5761 gaggggctga agtgctgcga cgtggacatg caggagggtt tcgtgctcgt gaccgggaag
     5821 ggatccaaac agcggatggt ccccgtgggg agcagggcca tagaggccgt gaggacctac
     5881 ctcctgacca gggggataga ggaccctctc tactgcaccg aaccgctgtt cttgggcagg
     5941 aggggaggtc ggctctgctc gcgcgtcatg aggaggatcg tcgagggctg ggcgatgcag
     6001 gcggccatcg ccaagagggt gagcccgcac gtgatcaggc acacgttcgc cagccacatg
     6061 ctcgacgccg gggccgacct caggtccatc caggagatgc tcgggcatgc aagcctctcg
     6121 accacccagc ggtacacgca cttgcatgtg gacaaactca tggaggtgta tgataaagca
     6181 catccgagag ccagggaggg atgatggaca tccacgcgac gacgatcctc tgcgtgaggc
     6241 gtttcggcag ggtggtcatg gccggggacg gccaggtcac catgagctcg gtcgtgctca
     6301 aggccaccgc gaagaaggtg cgcaagctct acgaggggaa ggtcctcgcg gggttcgcgg
     6361 gggccacctc ggacgcgttc acgctgttcg agcgcttcga ggacaagctc aaggagttcc
     6421 ggggcaacct cacccgggcc gccgtggaga tgggcaagga ctggcgcacc gacagggtgc
     6481 tcaggaggct ggaggcgctc atgatcgtcg cggatccatc caagaccttc ctcctctccg
     6541 gcatgggcga cgtggtcgag ccggacgacg aggtctgcgg cataggctcc ggcgggcact
     6601 acgccatggc ggcagccaag gcgctcatca ggcacacgga cttggacgcc ccgtcgatag
     6661 ccaagatctc catggagatc gcctccgaga tctgcatcta caccaaccgt gagatccacc
     6721 tcgaggagct cccatgaaag acctcacacc caggcagatc gtctccgagc ttgaccgcta
     6781 catcgtcggc caggacaggg ccaagcgggc cgtggcgatc gcgctgagga accgttggcg
     6841 gaggcagaac gtccccgagg agatgaggga cgagatctac cccaagaaca tcatcctcat
     6901 cgggcccacc ggcgtgggca agaccgagat agcacggcgg ctggcaaggc tcgcccatgc
     6961 ccccttcatc aaggtcgagg cctcgaagtt caccgaggtg ggctacgtgg gcagggatgt
     7021 cgagtccatc atccgcgacc tcatgcggac aggggtccag ctcgtccagg ccgaggagag
     7081 cgagaaggtc agggccaagg ccgtggaggc agccaaggag cgtgtccttg accacctgct
     7141 gcccgcgcag aaggacacgg agatccatgc cgagagccgg gagaagctca agaggatgct
     7201 ggaagagggg gtcctggacg acaggttcgt ggaggtcgag ttccgggaga cccagatgcc
     7261 gaccatcgag gtattctcgg gccaggggct cgaggagatg ggcatcgact tcaaggagat
     7321 gttctcgtcc atgctcccca agcgcacgaa aaggcgcagg atgagcgtga aggaggccct
     7381 cgagttcctc acgaaccagg agatcgcccg cctcgtcgac atggacaagg tgacccagga
     7441 ggcgcgaagg cgtgtcgaga acacgggcat cgtgttcata gacgagctcg acaagatagc
     7501 aggcagggaa tcgggcaggg ggcccgacgt gtcccgcgag ggcgtccaga gagacctgct
     7561 ccccatcgtg gaggggacca cggtcaacac caagtacggc cccgtgaaca cggaccacat
     7621 cctgttcata ggcgccggcg ccttccacgt gagcaagccc gcggacctca tccccgagct
     7681 ccaggggcgc ttccccatcc gcgaggaact cggaccgctc accaaggacg acttcgtgag
     7741 gatcctcacc gaaccgaaga acgcgctcac gaggcagtac caggcgctgc tcgccaccga
     7801 gggcatagag ctcaccttca ccgaggaagc gatcgcccag atagccgcca tcgcccagca
     7861 catcaacgac cagacggaga acatcggggc gcgcaggctc cacacggtga tggagaaact
     7921 cctcgaggag gtctccttcg cggcgggcga gagggccggg ccagtgaaca tcgacgggga
     7981 ctacgtgagg gaaaggctcg aacccctcat ggcgagggaa gacctgagca ggtacatcct
     8041 ctgaagacgc ttccatcgcc gagaaccgcc cgcggcggcg tgcctcgtgc ccacgcgcgg
     8101 catgcccctg acgggcccac ccgggagggc ctgcctgtac ggcaaggcgc ccctttgccg
     8161 ggcgttgccc ggacaaacgg ccgggtgtat gatcgtcgtc tgccggagga ctcatgatac
     8221 ccaagggaaa gttcgtctcc gagatccagg acggggaccg cgtcaaggcg gtcttcctgc
     8281 tcgcagaccg caggctgttg accgccagga acggcaagcc ctacgggagg gtggtggtct
     8341 ccgacaaaac cggcgacatc acaggcctca tttgggacga cgcacccgag cagatatcgg
     8401 ccgtctcgcc cggccacgtg gtgggagtgc gcgcgaccgc cgagtcctac gacaggaagg
     8461 tccagctgag gatcgagaag ataacgaggc tcgccgacaa ggaggtcgac ctctcggcgc
     8521 tcatccccac gagcccccgc gacctgcccg cgctggaggc cctcctcgac gaggccatag
     8581 acggcatgaa ggaccccttc ctgaaggccc tcgtgaaccg ggtattcgcc cggccgggag
     8641 tcagggagtc cttcgtgaag gcgcctgccg ccaagggcat ccaccacata ggcggtctcc
     8701 tcgagcacac cgtgaccgtg atcagggcgg cagacgcact gctgccggtg ttcgcccacc
     8761 tcgagctcaa cagggacatg atcatcgcgg gcgccatcct gcacgacctc gggaagatct
     8821 acgagtacac ctacggcaag gtcatcgaca tgacgccggt gggaaggctc ctgggccaca
     8881 tctacctctc cgcgcacatg gtggacgagg aggcctgcgc catcgagggc ttccccgagg
     8941 aactcaggct ccagatcctc cacatgatcc tgggccacca cggacagctc gagttcggct
     9001 cgccgaagct gcccatgacg agggaggccg tcttcctcca catgatggac gacctcgacg
     9061 ccaagctcac gggcatcacg tccctcatcc aggcgacacc cgaggacgag cccttcacgg
     9121 cgttctcgag cgtgtacaac cgcttcctct acacccgcac ctacagggac gaccccgggg
     9181 aagagaagtg agcctgcctg agcggttggg caggcttttt tcttgtcggc accccacgat
     9241 gcaccggccg ggcaaggggc gtgaccacac cgcccaggca ggcacccgcc aagacacccc
     9301 agggggcccg gcatatcgct aacttcacct gtcgccacgt ggccggccga gcctgcgacc
     9361 cgaggtgaac ggtcgttctc tgtgcggcat tcgcgagggc gaggatggtc gggctccgca
     9421 cggatggggg ctggggggtg tgcgcctctc ctggttgcac agaggctgtc tttgcgcagg
     9481 catggtcaag caccgggcac ggtcccgacc ggccgccagg accagggccc ccgagggagg
     9541 gccggggcct tcacccgtgc ctggagacga gaagggctcg cggtctcatc caaggtggtc
     9601 cagggtcgat ccatgcatgg tgcgtccgaa ccgtaccggg agggggcggt ctccgtcgcc
     9661 ccctctccgg ccgcaagacg tcacgatcac agatggtggg gtctcacacg cctgatgttc
     9721 agctcgtctt tggtcctgat gtagtccgtg tagatcctcc cgacgcagag ccccacggcg
     9781 aggacgaaga ggccgagcat gaacgccggc tcggtgccga gcctgacgtc gatccacctc
     9841 ccgacgaaga ggaagaggaa ggacgagacc acgagggtga aactccacgc gctcatgagg
     9901 atgatgtggt tgagcgtttt ggttttcatc cctgtccttc gggtctttcc gcctttaccc
     9961 ctcatggcgc cctccttccc cggagagtgc acgcccaccg acggggcgtg cacgccggaa
    10021 cggatatatg ctctcctcag ctcacaatgg tcttcaccgc ctcgatcacg ggcgcggtga
    10081 agagggccac gagcactgca tacaatgcga aggaaccgac ggcctcgctc atgtagatcc
    10141 tcgggtagcg gtgcttcagg gacacgatgg tgagcccgcc catgatcagg cagccgaggt
    10201 ggaagtacgg gaagttcgac ccgccggcgg ttgccgcagc ggcgaaggcg aaggtggacg
    10261 tgatgagaac gacgaagaga gcgacgacca ctgtctgcac tgtccttttc attgtcttgt
    10321 cctcccgaag agttcttttc gttccaccct tggtatagca gggggcgtgc cagggtttcc
    10381 cgccgcggcc caggcttttt atcatgttga tatctctggt ttttctgaac tcccgagcag
    10441 gcgcgccccc gtggaggccg taaaacgagg cgctccggtt caaagaatct ttcaagggac
    10501 tcgacgaaac gtcgaaagga ctcccgccct cgacgcgcgg cgtgctacaa tcgggatacc
    10561 ctgccgaacg ctgcggcctt gtcaaggtcc ggccggggaa acgccatgtc cgccggggga
    10621 ggccttcgga tgaaacggat ggacaagatg aacgggaaag ccaaacgccg cctaaggtcc
    10681 tcgtcttgct ggggagcccc aggagaaagg ggaacagcac gctgctcgca cagaggatag
    10741 cgaaaggggc cgagaaagcg ggcgcatccg tcgagtacgt ctaccttcac gggctcgaca
    10801 tcagggcatg cacgggctgc atgaagtgcc agcgcaaggg tgcgaaggga tgcgtgataa
    10861 aggacgacat gcaggacctc tacaggctca tgaaggagtg cgacgcgtgg gtggtcgcct
    10921 ccccggtcta ctggttcacg gtctcggcgc agctgaagct ctggatggac cgctgccttg
    10981 ccttcacctc ctacaccccg gacgcctttt cgggaaagcg catcgccatc gccatgacct
    11041 acggcggaga agacccgttc gattcgggct gcattaacgc cctgcgcacc ttccaggacg
    11101 cgtactccta cgtgggcgcc cgcatcgtgg gcatggtgta cggcagcgcc atggctgaag
    11161 gcgagatcgc gaaagacgcc ggggtcttgt cgaaggccga ggacttgggc cacctcctcg
    11221 ttgcggcaga cggatgaggc cttcgggtcg acccgggcaa agagacaccc tctacctgcg
    11281 ccgggcgacc tcgacccgtt cacgtactaa gcctcacgcc cgaggccggg agggacggcc
    11341 ggaacccccg aaggcccggg aaccccaccc ggacgaacag ggaacacccc gtcactcttt
    11401 taggacacct gtctccaagg gggaaaaggg tcagccatga ggtatccgca agctctcggg
    11461 gaatcccagg aggatcgcct cccggaggca ttcctctttg agcggaggcc ccgggcaggg
    11521 agaaccgccc accgtttttt accggcgccc ttgcccgcac ccgtcgagac cgagatcagg
    11581 ccgcccaccg ctgagcctgg cgcccagacg gccctgacgg ggcagggctc cccagggatc
    11641 agcgctcaaa acgggtgggg atccccctcc ggcctgagaa agacggcctc atccctgcgg
    11701 caggacccag aagacgtacc acctccgcgc ccactcgtcc acgtagacca cggccgggtc
    11761 cgacaagagc gcctcacccc agaggcccga agagacctcc ttgaccgtga acccggggcg
    11821 cgaatccgca tccacgccca ggcccccgtc gtcctcggca aagccgtgcg cgccgcacgg
    11881 gcaggaggcc caccaggcgt cgatcccctc tatctccacc agataccgcc tcgtgcagaa
    11941 ggcgcacctc acgaccaggt agtccatccc ctcatgtgtg acccctctcg ggtggtcttg
    12001 tcaatgcctg ccgccgcttg actgtcgtgg ggaaatgggt cacaagaacc cgtaagggga
    12061 ggacgacagc catggcgaga gttcgcttcg aggacacttc ctgcgggatc gacgcggccg
    12121 tgacaaaggc gctcgactcc ttcggttcgc cgcggtccct gctcaggcgc tcgggggccg
    12181 tgttcctcaa ggtcaacctc atcgactcca agccccacgt gttcacctcc gtggaggtgg
    12241 tcagggcgat cgtcacccgc ttcctcgcgg gcggcgcccg ggccgtctac gtgatcgaga
    12301 acagcaccca gggcaacttc acgaggctcg tgttcgaggc cacgggcatg aggaggatgt
    12361 gcgaggagac aggggcgatc cccgtgtacc tggacgagac cgcggagatg cccatgtacc
    12421 tggagcggct cgggagcttc gtcaggatcc ccgacttcgt ccacgagcac ctcgtgagga
    12481 aacgtgacga gaacctctac atcagcgtgc ccaagctcaa gacccactcc atgtccacgg
    12541 tcacgctcgg gatcaagaac cagttcggtc tcctccacca cagaagccgc atccccgacc
    12601 acaacttccg gctgcaccag aagatcgccg acatctacgc gaacatccgg cctgacctca
    12661 ccctcatcga cgccacggag gcgaccaacc acggccacta ccccgcaacg gggttcgcaa
    12721 gcgagtgcgt ggtgcccctc gatctcgtca tcgcggg
//
LOCUS       DAOSCR010000006        12753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_3315_length_12753_cov_3.913136, whole genome shotgun sequence.
ACCESSION   DAOSCR010000006 DAOSCR010000000
VERSION     DAOSCR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12753
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_12753_cov_3.913136"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..676)
                     /locus_tag="PLT69_00485"
     CDS             complement(203..676)
                     /locus_tag="PLT69_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOJ12957.1"
                     /translation="MERSWQDVLDRFHYGIYLVTIASDGMYNAMVASWVAQCSHEPPL
                     ITLAIRNTRLSREQIMNSGAFTINVLPVDALQDIGRFKISDWRRKLEGVEYELSPGGA
                     PVLKDAVGYLDCTVERAIETGDHTLFVARVTSGGIVKDGEALTTREYQGRYRGDR"
     gene            complement(808..1788)
                     /locus_tag="PLT69_00490"
     CDS             complement(808..1788)
                     /locus_tag="PLT69_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOJ12958.1"
                     /translation="MDDTQIIELGKEAVRIEIEGLSALLDQVGPAFAKAVRLIAGLEG
                     KAVLTGVGKSGIIARKVASTMVSIGIPAVFMHPVDGLHGDLGSIMGTDAVVIFSNSGR
                     TREILDLLDALAKIGVPVVSVTSDPSSPMAERSDIVLQCRVAREACNLNLAPTASTTA
                     QLALGDALAVVVSRIKGFDTEAFRALHPAGELGRQLMARISQIMITGRRIPLVAPDTP
                     LERLVREMTDKGLGFTLVADAGDVVGMITDGDLRRVLLKHGNRLEGLVALDIMTPNPK
                     RIEQGKLAIDALDMMEKHQITSLVVTDEQGVFQGVVHLHDLLGRGKLGFR"
     gene            complement(1869..3170)
                     /gene="rimO"
                     /locus_tag="PLT69_00495"
     CDS             complement(1869..3170)
                     /gene="rimO"
                     /locus_tag="PLT69_00495"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ12959.1"
                     /translation="MKRFSMVSLGCPKNLVDSEYICERFVQAGYTLVPGAEDADLVVI
                     NTCAFLTSAVEESITALLEAVDSGREVICAGCLVSRYGRELLHEMPEIVLFAGPGTYE
                     GIVEAYEQGAGILEPVFEGVVKRAFTSNPHSAYVKVSEGCSNRCGYCLIPSIRGPLVS
                     KPPEEVLEECRILAGQGVVEVILVAQDLGNYGTDLGMTDGLARLLDMVSTVEGLEWIR
                     LMYVHPASLSGAVVEHLGKNPKVCPYIDMPIQHVSDRVLEAMGRKGGAHAVRRAFDLL
                     GSASPEVWVRTSLMVGHPGEDDEAFEELERFVASGRVHHLGVFVYSPEEGTRSASLRG
                     RPARAVAARRRRRIMGIQQEVSKKRLKNMVGGEIPVLVEGPHPETDLLASGRAAFQAP
                     EVDGMVVITEGDARPGTIVAVQVTRAWEYDLVGRIVRVVTG"
     gene            complement(3180..4415)
                     /gene="glgC"
                     /locus_tag="PLT69_00500"
     CDS             complement(3180..4415)
                     /gene="glgC"
                     /locus_tag="PLT69_00500"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOJ12960.1"
                     /translation="MAYVNPNDVEVVILAGGRGERLYPLTKDRAKPAVPFGGMYRIID
                     FTLSNCVNSGLKKIYVLSQYKSHSLNRHIQRGWLPFFSYPMGEFIYDIPAQQRIGSSW
                     YEGTADAVFQNIYSLQQTNTPLTLILSGDHVYQMDYREMIGFHLDTKADVTIGVVAMP
                     VETASQFGVVRADEAMRVVGFQEKPKKDPYTMPGDRSKVLVSMGIYVFHTSTLIRRLV
                     EDAKLKTSSHDFGKDIIPRMVKNAAVMAFPFKDIMGESYWRDIGTLDAYYEANMDLVS
                     VTPKCNLYHTSWPVHTVYSPYPPSKMVFAGGEDGKRIGQVLDSIVCGGSIVSGGRVER
                     SIISPNVRVNSYAEIEDSILMEGVEVGRHCRIRRTIVDKRVKIPPRTTIGYDAEEDAR
                     RFDISPGGIVVVPKNTVFD"
     gene            complement(4417..4773)
                     /locus_tag="PLT69_00505"
     CDS             complement(4417..4773)
                     /locus_tag="PLT69_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ12961.1"
                     /translation="MKRILIVEDDRSIQELLKQELNDEGYATSIAGNGREALDLLRSD
                     AKFDLIVLDLRMPAMDGFETMGHILKLKEETPVVIHTAYPSYKNDALAQAADAFVVKS
                     HDLTALKKTIFSLIGK"
     gene            complement(4871..5260)
                     /locus_tag="PLT69_00510"
     CDS             complement(4871..5260)
                     /locus_tag="PLT69_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ12962.1"
                     /translation="MRFRKARRDRRTSVLDVTPLVDVVFLLQIFFLLTLGSPLVLSEV
                     DLPATISGKNVVSPASTVIVLADGVVVDGSKAAEEAKAIASLPKGRQVVVMAPKGILY
                     ERVMKVLDTLRLTGHARISLATKPVGR"
     gene            complement(5214..5876)
                     /locus_tag="PLT69_00515"
     CDS             complement(5214..5876)
                     /locus_tag="PLT69_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ12963.1"
                     /translation="MTLWEFFLKGGPLMWPLLVCSVVALAIIVERMFALRQSRIIPRD
                     LIEEVERLVRMGRLGDIEQLLKRNSSPICPVIMVAIKNAGMRREIIKDYMEVAGAGEA
                     YAIERYLDILGIIAAIAPLLGFLGTVTGMLASFQAVAGSAGGANSSQLLAAGISEALI
                     TTVAGLAIGIPVFVCYRLLVARSDYLVMEMEKTSARILEYLKGDAHEVQKGAKGQEDL
                     GS"
     gene            6169..7170
                     /gene="ricT"
                     /locus_tag="PLT69_00520"
     CDS             6169..7170
                     /gene="ricT"
                     /locus_tag="PLT69_00520"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOJ12964.1"
                     /translation="MTLMEQESRDIRLVCVRLENIPLTAVYEAGGFEVSQGDRVLVNT
                     ESGQCVARVIGFADPAKIPELENISPIVRPLGAEDLERLERIRHKERECFEFCQQRIA
                     ARGLPMKLIRVEQFFEENKITFYFSAEGRVDFRDLLRDLVERYRTRIELRQVGTRQES
                     AMLGGIGSCGRELCCATFLTNFQRISVKMAKYQNMTLNPTKISGLCGKLKCCLAYEKE
                     SYANLIENLPKPGRKVYLAEGEATVVSINVINQTFVAKLSDRRFVKARVEDVLTEEEF
                     KNQPQRKPQLPEPEPSVSAEAGADDGLPMEPLVVPQQKPKKKRPRHRGARKKRSPKA"
     gene            7167..9083
                     /gene="metG"
                     /locus_tag="PLT69_00525"
     CDS             7167..9083
                     /gene="metG"
                     /locus_tag="PLT69_00525"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ12965.1"
                     /translation="MSRYFYVTTPIYYVNAKPHLGHVYTTVIADVLARFHKLSGDVVF
                     FLTGTDEHGDKIMETARSLGTTPRELADENSQRFRDLWPLLEVRHDRFIRTTEPDHVQ
                     TVQRILQEVYDRGDIYFGEYGGYYCTGCERFMTETEMEDGACPIHKRPLSFVQEKNYF
                     FRMEKYRPWLVEHIQANPDFIRPERYRNEVLGMLREPIDDLCISRPKSRLTWGIELPF
                     DRDYVCYVWFDALINYLTGIGYPDGAGFDPLWKEAQHLVGKDILKPHAVYWPTMLKAI
                     GLEPYRHLNVHGFWNVDAAKMSKSLGNVVDPLDLKDKYGVDAFRYFLVRDMVFGLDAN
                     FSEEALIERYNADLANDLGNLVSRSTKMVTRYFDGVLPAPSGDTTDMERELVDRASVV
                     VGEFKERMEALELHTALISVWSLISAANRYVDRASPWELAKKDRARLSTVMYHLVETI
                     RIVAVLINPVMPLASRKILDLVGWTGEVSLNDVLPFGKLTPGVSVSPAEALFPRVERL
                     AKAACDAAPTKQQKEEKRPGGPSNLVDISRFGEIEIRVGRVLEASRVEKSDKLLALTV
                     DVGRRIRIVAGIGTSYTPEELAGKHIAVVTNLKPATLMGLKSEGMLLATDCEEGGLAL
                     VGFDRTPKVGAKVR"
     gene            9094..9438
                     /locus_tag="PLT69_00530"
     CDS             9094..9438
                     /locus_tag="PLT69_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12966.1"
                     /translation="MQKVPIELVKPGMVLAKTVCNESGMALCGEGTELTDTIIERLKR
                     MNISHVVLKGHPVDMGESKTTEEKLADLEARFERCRKDPLMERVLEAARAALVSLDEE
                     RAEQEQREEGSR"
     gene            9435..10271
                     /locus_tag="PLT69_00535"
     CDS             9435..10271
                     /locus_tag="PLT69_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ12967.1"
                     /translation="MSTKVQHLKVLVRRIQSLPTLPQVVHKLMTMVESPDVSPKDVGK
                     LISTDQVLGARVLRLVNSPFYGFPGRISSISHAIILLGFNVIKGVVLSASVFELMERT
                     MVGLWEHSLGSAVAAGTLGKALGMSDPEEISTAALLHDIGKVLVKVSLPDDYAEIERL
                     VTEERISFREAELKALKVDHAQIGQWLSREWNLPERLSIPITWHHEPEEAVKFKDRCA
                     AVHVSDALVRACGVGNGGDPWVPKISQWAWETLGMDRVDLKDLMKRIMEELEVVEMYH
                     QS"
     gene            10285..11205
                     /locus_tag="PLT69_00540"
     CDS             10285..11205
                     /locus_tag="PLT69_00540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ12968.1"
                     /translation="MKAIYITDWPDNAEARDLLESHVSSLVTVTPTQDIISLVYMDPP
                     DLILVDERILAGAGRKTLEEFMGTIVGHVPVAAVLEGYDREGDEWMDLPIDDYIVLTD
                     QERAMQWRLAFMAKRSTREMDLNPLTRLPGNESIIRRIQEVIDAREEAAIAWVDIDNF
                     KPFNDRYGFSRGDEVILATARIITNAAREITPEKVFVGHVGGDDFVFVCPAGMVGRFC
                     EDVIDRFDTVIKNFYNDEDVETGGIVSKGCDGVERKYGAMTVSIAVVLNVNGRYSHYG
                     QAAQDASDIKKYLKGLKGSNYMIDRRAGRS"
     gene            11202..12497
                     /gene="purB"
                     /locus_tag="PLT69_00545"
     CDS             11202..12497
                     /gene="purB"
                     /locus_tag="PLT69_00545"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOJ12969.1"
                     /translation="MIQRYTRKRLAEIWSDENRYRKWLDVELAVCEAWAELGRIPPES
                     METIRRRAAFSIERIEEIEAVTRHDVIAFVSAVAEHVGEDARFIHLGLTSSDVLDTAY
                     ALLLKESGMVIVEDIQDMRAVLKDRAYEFKDLPAIGRSHGVHAEPITFGLKFALWYAE
                     MGRNLERMMRAIDRISFGKISGAVGTFANVPPEVEASVCARLGLKPAPISTQVLQRDR
                     HAEFFCTLAIVGASAEKIATEIRHLQRTEVLEATEPFGAGQKGSSAMPHKKNPVLTEN
                     ICGLARLLRGYAMSALENVALWHERDISHSSVERVIGPDATTVLDFMLARLTGVLRGL
                     EVHTDNIRRNLELTGGLWHSQNVLLALVDRGVARDTAYTWVQRNALKAWETKADFKGL
                     LLQDPDVARTLDPRTIDDLFDLSHHLAHIDAIFERVFNE"
     gene            12490..>12753
                     /locus_tag="PLT69_00550"
     CDS             12490..>12753
                     /locus_tag="PLT69_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOJ12970.1"
                     /translation="MSDIDDLRDIVKRDAVRFGKFILSSGKESDLYVDLRMVTLHPRG
                     ASLIGRLIFDRIRDLEIDAVGGMSIGADPIAVSVSLAAFSEGRQ"
BASE COUNT         2418 a         4142 c         4017 g         2176 t
ORIGIN      
        1 gccgcgaacc ctcggtcccc cggccagggg cctcttgacg ggccgacaat gctccctcgg
       61 aaggatcaac accgcactcc ctcagctctt gcccgctctg tgtggcatct ccatgggtgt
      121 gcgggtgatc ggggcgtcct gcagacagaa cacctcgaac ccgcggacag gggggctcgc
      181 accctgtcgg cccgggtgca acctacctgt cacccctgta cctgccctgg tactcgcgcg
      241 tcgtgagggc ttcgccatcc ttgacgatcc cgcccgaggt cacgcgcgcg acaaagagcg
      301 tgtggtctcc tgtctcgatg gcgcgctcga ccgtgcagtc gaggtacccc accgcatcct
      361 tgaggaccgg agccccaccc ggcgaaagct cgtactccac cccctcgagt ttgcgcctcc
      421 agtcgctgat cttgaaacgt cctatgtcct gcagggcgtc caccggcaac acgtttatgg
      481 tgaacgcccc cgagttcatg atctgctccc tggagagcct cgtgttcctg atcgcgagcg
      541 tgatcagggg cggctcgtgg gagcactgcg caacccacga tgcgaccatg gcgttgtaca
      601 tcccgtcgga tgcgatcgtc acgaggtata tgccgtagtg gaacctgtcc aagacgtcct
      661 gccacgatct ttccatgacg agcccccttt ctctcggccc ccgaaccatc ggccgtgttc
      721 ctcctatagg gcacacggct cccggcggaa agcccaggtc cacaagcggg tgggcggcgc
      781 aggccgcgcc ctgcttcggg ccccttctca cctgaagccg agtttgcccc tgcccaagag
      841 atcgtgaagg tggaccaccc cctggaagac accctgctcg tcggtgacca cgagcgaggt
      901 tatctggtgt ttctccatca tgtcgagcgc gtcgatggcg agctttccct gctcgattcg
      961 cttcgggttg ggcgtcatga tgtcgagcgc gacgagcccc tcgagcctgt tgccgtgctt
     1021 gagcaggacc cttctgaggt cgccgtccgt gatcatgccg acgacgtccc cggcgtcggc
     1081 gaccagggtg aacccgagcc ccttgtcggt catctcccgg acgagccgct cgagcggggt
     1141 gtccggcgcc acgaggggga tgcgccggcc cgtgatcatg atctgcgaga tccgcgccat
     1201 gagttgcctg ccgagctcgc ccgccgggtg gagcgcccgg aaggcctcgg tatcgaaccc
     1261 cttgatcctg gatacgacca ccgccagcgc gtcgccgagg gcgagctgcg ccgtggtgga
     1321 ggccgtcggc gcgaggttga ggttgcacgc ctcgcgagcg accctgcact ggagcactat
     1381 gtcggaccgc tccgccatgg gcgaggaagg gtccgaggtg acggacacga cgggcacccc
     1441 gatcttcgca agggcgtcca gaaggtccag gatctcgcgc gtcctgccgc tgttggaaaa
     1501 gatcaccacc gcatccgtgc ccatgatgga gccgaggtct ccgtgaaggc cgtcgaccgg
     1561 gtgcatgaat accgcgggta tcccaatgct caccatggtg gacgcgacct tcctcgcgat
     1621 gatcccggac ttcccgacgc cggtgagcac cgccttgccc tcgagcccgg cgatgaggcg
     1681 tacggccttg gcgaaggcgg ggccgacctg gtcgaggagg gcgctcaacc cctcgatctc
     1741 gatacgtacc gcctcctttc ccagttctat gatctgagtg tcatccatgc cacgctccaa
     1801 agatcagccc tcccgggggc ggccgaaaga cccgcggccg tcctcgcgtc gacgatccca
     1861 cctgaagatc accctgtaac gacccgaacg atcctcccca cgagatcgta ctcccacgcc
     1921 cgggtgacct gcaccgccac gatcgtccca ggcctggcgt caccctcggt gatcaccacc
     1981 atgccgtcca cctcgggcgc ctggaaggcg gcccttccgc tcgcgaggag gtccgtctcg
     2041 gggtggggac cctccaccag tacaggtatc tcgccgccca ccatgttttt caacctcttt
     2101 ttcgacacct cctgctggat gcccatgatc cttcgccttc tccttgcggc gacggccctg
     2161 gcaggcctgc cccggaggga cgcgctcctc gtcccctcct ctggcgagta gacgaagacg
     2221 cccaggtgat ggaccctgcc ggaggccacg aagcgctcga gctcctcgaa ggcctcgtcg
     2281 tcctcacccg ggtgacccac catgaggctc gtcctcaccc agacttccgg agacgccgaa
     2341 ccaaggagat cgaaggccct cctcaccgca tgggcgccgc ctttcctgcc catggcctca
     2401 agcaccctgt cggagacgtg ctgtatgggc atgtcgatgt agggacatac cttcgggttc
     2461 ttcccgaggt gctccacaac cgccccggac aatgaggcgg ggtgcacgta catgagcctg
     2521 atccactcca gcccctccac ggtgctcacc atgtcgagga gcctggccag gccatcggtc
     2581 atgcccaggt ccgtgccgta gttccccagg tcctgggcca cgaggatcac ctcgaccacg
     2641 ccctgtcctg caaggatgcg gcattcttcc aggacctcct cgggcggctt gctcacgagg
     2701 ggccccctga tggacgggat gagacagtac ccgcacctgt tggagcaccc ctcgctcacc
     2761 ttcacgtagg cgctgtgggg gttcgaggta aaggccctct tcacgacccc ctcgaagacc
     2821 ggctcgagga tacccgcccc ctgctcgtag gcctcgacga tgccctcgta cgtacccggc
     2881 ccggcaaaca ggactatctc gggcatctcg tgcaagagct ccctcccgta gcggctcacc
     2941 aggcaccctg cgcagatcac ctccctgccg gaatccaccg cctccaagag ggccgtgatc
     3001 gactcctcca ccgcggaggt caggaaggcg cacgtgttga tcaccacgag atcggcgtcc
     3061 tcggcgccgg ggacgagcgt atagcccgct tgcacgaacc tctcgcagat gtactccgag
     3121 tccacgagat tcttggggca gccgagactc accatcgaga agcgcttcat cgatgggctt
     3181 cagtcgaaca cggtgttctt cggcacgacc acgatgccgc cgggcgagat atcgaagcgc
     3241 cgggcgtctt cctcggcgtc gtacccgatg gtggtgcgag gcgggatctt cacccgcttg
     3301 tcgacgatgg tacgcctgat cctgcagtgc ctgccgacct ccacgccctc catgaggatc
     3361 gagtcctcga tctcggcgta gctgttgacc cgcacgttcg gggagatgat ggacctctcc
     3421 accctgccgc cggacacgat ggacccgccg cagacgatcg agtcgagcac ctggcctatc
     3481 ctcttcccgt cctctccgcc ggcgaacacc atcttcgagg gggggtacgg gctgtacacc
     3541 gtgtggacgg gccaggaggt gtggtagagg ttgcacttgg gcgtgaccga gacgaggtcc
     3601 atgttggcct cgtagtatgc gtcgagggtc ccgatgtccc tccagtaaga ctcacccatg
     3661 atgtccttga agggaaacgc catgacggcc gcgttcttga ccatgcgggg gatgatgtcc
     3721 ttcccgaagt cgtggctgct cgtcttgagc ttggcatcct ccacgagcct ccgtatgagc
     3781 gtggaggtgt ggaagacgta gatccccatg gacacgagca ccttgctccg gtccccgggc
     3841 atggtgtacg ggtctttctt gggtttctcc tggaacccca caacccgcat ggcctcgtcc
     3901 gccctgacca ccccgaactg ggacgccgtc tccacgggca tggccacgac gccgatcgtc
     3961 acgtcggcct tggtgtccag gtggaaaccg atcatctccc ggtaatccat ctggtagacg
     4021 tggtcgccgg agaggatgag cgtcagcggg gtgttcgtct gctggagaga gtagatgttc
     4081 tggaacacgg cgtcggccgt tccctcgtac cagctcgacc cgatacgctg ctgggcgggt
     4141 atgtcataga tgaactcgcc catggggtac gagaagaagg ggagccaccc gcgctggatg
     4201 tgtcggttca gggagtggga cttgtactgg gagagcacat agatcttctt gagaccggag
     4261 ttcacgcaat tggacagcgt gaagtcgatg atccggtaca tgccgccgaa gggaactgcg
     4321 ggcttggccc ggtccttggt caggggatag agcctctccc ccctgcctcc cgcaagaatg
     4381 accacctcca catcgttcgg gttgacgtat gccatttcat tttccaatca gagaaaatat
     4441 cgtctttttc agggcggtga gatcgtggga cttcaccacg aaggcgtccg ccgcctgggc
     4501 gagggcgtcg ttcttgtagc tcgggtatgc ggtgtggatg accacggggg tctcctcctt
     4561 cagcttgagg atgtggccca tggtctcgaa cccgtccatg gccggcatgc gcaagtcgag
     4621 cacgatgagg tcgaacttgg cgtccgagcg caagagatcg agcgcctccc tgccgttccc
     4681 cgcgatactc gtcgcgtacc cctcgtcgtt gagctcctgc ttcaggagtt cctgaatgct
     4741 ccggtcatct tcaactatca ggatacgctt cattttcaac cagtaatatt aatgagatga
     4801 gcataccatg tcaaacccta tcagggaatc tccctcgccg ggggctgaaa ggacaagccg
     4861 cctgcgcccc tcagcggccc accggctttg tcgcgaggga gatgcgggca tggcctgtca
     4921 ggcgcagggt gtcgaggacc ttcatgaccc tttcatagag gatgcccttg ggggccatga
     4981 ccacgacctg cctccccttg ggcagcgagg cgatcgcctt ggcctcctcg gcggccttgc
     5041 tcccgtccac cacgaccccg tcggcgagca cgatcaccgt gctggccggc gagacgacgt
     5101 tcttcccgct gatcgtggcc ggcaggtcca cctcgctcag cacgagcggg ctgcccaggg
     5161 taagcaggaa aaagatctgg agcaggaaga cgacgtccac caagggggtg acgtcaagaa
     5221 ccgaggtcct cctgtccctt cgcgcctttc tgaacctcat gggcgtctcc cttaaggtat
     5281 tcgaggatcc tggcggaggt cttttccatc tccatgacga ggtagtcgct gcgggccacg
     5341 agcaggcgat agcacacgaa gaccgggatc cctatggcga gcccggccac cgtcgtaatg
     5401 agcgcctcgg agatgcccgc cgccaagagc tggctggagt tcgccccgcc tgcgctgccg
     5461 gccacggcct ggaaggacgc gagcatgccc gtgacggttc cgaggaaccc gagcaggggc
     5521 gctatcgcgg cgatgatgcc gaggatgtcc aggtacctct cgatggcgta ggcctctccc
     5581 gctcccgcca cctccatgta gtccttgatg atctccctgc gcatgccggc gttcttgatc
     5641 gcgaccatga tgacggggca gatcggggaa gagtttctct tgaggagctg ctcgatgtcg
     5701 ccgagcctcc ccatgcgcac gaggcgctcc acctcctcga tcaggtcccg cgggatgatc
     5761 cggctctgcc tgagggcgaa catcctctcg acgatgatgg ccagggcaac caccgaacag
     5821 acgagcaacg gccacatgag cggtccgccc ttgagaaaga attcccacag cgtcatagcc
     5881 cctcctgctc gttcggcgat accatacgcg tcttaccaca tcgtctcgag catgaaaagg
     5941 cctctcgccc ccttaagcgc cccgtctcgt cgtccgaggc agcaccgccc gcgcccatcc
     6001 ccccgccaag gcacggcaat cacgaccttt cagcccctcg caccggagaa caccccggag
     6061 aataagcccg gcccttcgcc ccccggacac gaccagcacc ctttgacccg cctcggttct
     6121 cttccctgag aaagataggc ttgctatgct cgcgccatgg ttgctaacat gaccctaatg
     6181 gagcaggaat cgagagatat cagactcgtg tgcgtgagac tcgagaacat ccccttgacg
     6241 gccgtctacg aggcgggcgg gttcgaggtc tcccaggggg accgcgtgct ggtgaacacc
     6301 gaatccggcc agtgcgtggc ccgcgtgatc gggttcgcgg acccggccaa gataccggaa
     6361 ctcgagaaca tcagcccgat cgtcaggccg cttggcgccg aggatctcga gcgtctcgag
     6421 cgcatcagac acaaggagcg ggagtgcttc gagttctgcc agcagaggat agccgcccgg
     6481 gggcttccca tgaagctcat acgtgtcgag cagttcttcg aggagaacaa gatcaccttc
     6541 tacttctccg cagagggccg ggtggacttc agggacctct tgagggatct ggtcgaacgc
     6601 tatcgaaccc gcatcgagct caggcaggtg ggcacccggc aggagtcggc catgctcggg
     6661 ggcataggga gttgcggcag ggagctgtgc tgcgcgacgt tcctgacgaa cttccagcgc
     6721 atctcagtca agatggccaa gtaccagaac atgaccctga accccaccaa gatctccggc
     6781 ctgtgcggaa agctcaagtg ctgcctcgcc tatgagaagg agtcgtacgc gaacctcatc
     6841 gagaacctgc cgaaacccgg caggaaggtg tacctggcag agggggaggc cacggtggtg
     6901 agcatcaacg tcatcaacca gaccttcgtg gcgaagctct ccgacaggcg cttcgtgaag
     6961 gcccgcgtgg aagatgtcct gacagaggag gaattcaaga accagcctca acgcaagccc
     7021 cagctcccgg agccggaacc ctccgtgagc gcggaggcgg gggccgacga cgggttgccc
     7081 atggagcccc tcgtggtacc acagcagaag ccgaagaaaa agaggcccag gcaccgcggc
     7141 gcgcgcaaga aaaggagccc gaaggcatga gcaggtattt ctacgtgaca acgcccatct
     7201 actacgtgaa cgcgaaaccg cacctcggcc acgtctacac gaccgtgatc gcagacgtac
     7261 tggcccgttt ccacaagctt tcgggcgacg tggtcttctt cctcaccggc accgacgagc
     7321 acggggacaa gatcatggag accgcccggt cgctcggcac caccccaaga gagctcgcgg
     7381 acgagaacag ccagcgattc agggacctgt ggccgctgct cgaggtgagg cacgaccgct
     7441 tcatccggac cacggaaccg gatcacgtgc agacggtcca gcgcatcctc caggaggtat
     7501 acgacagggg ggacatctac ttcggcgagt acggcggcta ctactgcacg ggatgcgagc
     7561 gattcatgac cgagaccgag atggaggacg gggcctgccc gatccacaag cggccgctct
     7621 ccttcgtcca ggagaagaac tacttcttcc gcatggagaa gtacaggccg tggctggtcg
     7681 aacacatcca ggccaacccc gatttcataa ggccggagag gtaccggaac gaggtgctcg
     7741 gcatgctcag ggagcccatc gacgacctgt gcatctcgcg cccgaagtcg cggctcacct
     7801 gggggatcga gctccccttc gacagggatt acgtgtgtta cgtctggttc gacgccctca
     7861 tcaactacct tacgggcatt ggctaccctg acggcgccgg gttcgacccc ttgtggaagg
     7921 aggcccagca cctcgtgggc aaggacatcc tcaagcccca cgccgtgtac tggccgacca
     7981 tgctgaaggc catcggcctc gagccctacc gtcacctcaa cgtccacggg ttctggaacg
     8041 tggacgccgc gaagatgtcc aagagcctgg gcaacgtggt cgaccccctg gacctcaagg
     8101 acaagtacgg cgtggacgcc ttcaggtact tcctcgtgcg ggacatggtc ttcggcctcg
     8161 acgcgaactt ctccgaggag gcgctcatcg agcgctacaa cgcggacctc gccaacgacc
     8221 tcgggaacct ggtgagcagg agcaccaaga tggtgacgag gtacttcgac ggtgtcctcc
     8281 cggccccgtc cggggacacc acggacatgg aacgtgagct cgtggaccgg gcatctgtgg
     8341 tcgtgggcga gttcaaggaa aggatggagg ccctcgagct ccatacggcc ctgatctctg
     8401 tctggtcgct catctccgcg gcgaacaggt acgtggaccg ggcctccccc tgggagctcg
     8461 caaagaagga cagggcgagg ctctccacgg tcatgtacca cctcgtggag accatccgca
     8521 tcgtggcggt cctcatcaac cccgtgatgc cgctcgcctc gaggaagatc ctcgacctgg
     8581 tggggtggac gggcgaggtg tccctcaacg acgtccttcc cttcgggaag ctgacgcccg
     8641 gcgtgagcgt gagcccggcc gaggcgctct tcccgcgggt ggagcggctg gccaaggccg
     8701 cctgcgacgc cgcgcccacg aagcagcaga aggaagagaa acgccccggc ggcccttcca
     8761 acctcgtgga catcagccgg ttcggcgaga tcgagatcag ggtgggccgg gtgctggagg
     8821 cctcgagggt ggaaaaatcg gacaagctct tggccttgac ggtggacgtg gggaggcgga
     8881 tcaggatcgt tgcggggatc gggacctcgt acaccccgga ggaactggca ggcaaacaca
     8941 tagcggtggt gaccaacctg aagcccgcca ccctcatggg gctcaagagc gaggggatgc
     9001 tccttgcgac cgactgcgag gaaggcgggc tggcgctcgt gggcttcgac cgcaccccca
     9061 aggtgggcgc gaaggtgcgc tgagggtgac gccatgcaga aggtcccgat agaactggta
     9121 aaaccgggca tggtactcgc caagaccgtg tgcaacgagt cgggcatggc cctgtgcgga
     9181 gaggggacgg agctgaccga cacgatcatc gagcgtctca agcgcatgaa tatctcgcac
     9241 gtcgtcctca agggccaccc cgtggacatg ggagagagca agaccaccga ggagaagctc
     9301 gccgatctcg aggcgaggtt cgagcgatgc cgcaaggacc ctctcatgga aagggtgctc
     9361 gaggccgctc gagcggcgct cgtctccctc gacgaggagc gggcggagca ggaacagaga
     9421 gaggagggct cacggtgagc accaaggtgc agcacctcaa ggtcctggtg cgtcgcatcc
     9481 agagcctgcc cacgctgccg caggtggtgc acaagctcat gaccatggtc gagagccccg
     9541 acgtctcgcc gaaggatgtc gggaaactca tctccaccga ccaggtgctg ggggccaggg
     9601 tcctcaggct cgtgaactcc ccgttctacg ggttcccggg gaggatctcc tccatcagcc
     9661 acgcgatcat cctcctcggc ttcaacgtga tcaagggcgt ggtcctgtcc gcgtcggtct
     9721 tcgagctcat ggagaggacc atggtgggcc tctgggaaca ctccctggga agcgccgtcg
     9781 cggcggggac cctgggcaag gcgctcggca tgagcgaccc ggaggagatc tcgaccgccg
     9841 cgctcctcca cgacatcggg aaggtcctcg taaaggtgtc cctccctgac gattacgcag
     9901 agatcgagcg gctcgtcacc gaggagagga tctccttcag ggaggccgag ctcaaggccc
     9961 tcaaggtgga ccatgcccag ataggccagt ggttgagcag ggagtggaac ctgcccgaga
    10021 ggctctccat ccccatcacc tggcaccacg aaccggagga ggcagtcaag ttcaaggacc
    10081 gctgcgccgc ggtccacgta tccgacgccc tggtgagggc atgcggggtg ggaaacggcg
    10141 gcgacccatg ggtcccgaag atctcgcaat gggcgtggga gacgctcggg atggaccgcg
    10201 tggacctcaa ggacctcatg aagaggatca tggaggaact cgaggtggtg gagatgtacc
    10261 accagtctta ggagtgaacc ccggatgaag gcgatataca tcaccgactg gccggataac
    10321 gccgaggcca gggatctcct cgagtcccac gtaagctcgc tggtgaccgt cacaccgacg
    10381 caggacatca tctccctcgt ctacatggac ccgcctgatc tcatcctcgt ggacgaacgg
    10441 atcctggcag gcgccggccg gaagaccctc gaagagttca tgggtaccat cgtcggccac
    10501 gtgcccgtgg ccgccgtact cgaagggtac gacagggaag gcgacgagtg gatggatctc
    10561 cccatcgacg actacatcgt gttgaccgac caggaaaggg cgatgcaatg gcgcctcgcc
    10621 ttcatggcca agcggtcgac gagggagatg gacctgaatc ccctcacgag gctgcccggg
    10681 aacgagtcca tcatccgcag gatccaggag gtcatcgacg ccagggagga ggctgccatc
    10741 gcctgggtgg acatagacaa cttcaagccg ttcaacgacc gctacgggtt ctctcggggg
    10801 gacgaggtca tcctggcgac ggcgaggatc atcacgaacg cggcgaggga gatcacgccc
    10861 gagaaggtct tcgtcggcca cgtcgggggt gacgacttcg tgttcgtctg ccccgccggg
    10921 atggtcggca ggttctgcga ggacgtgatc gacaggttcg acaccgtgat aaagaacttc
    10981 tacaacgacg aggacgtgga gacagggggc atcgtgtcca agggctgcga cggcgttgaa
    11041 aggaaatacg gcgccatgac cgtgtccatc gcggtcgtgc tcaacgtcaa cgggaggtat
    11101 tcccattacg gccaggccgc gcaggacgcg tccgacatca agaagtacct caagggtctc
    11161 aaggggagca actacatgat agacaggagg gcgggaagga gttgatacag aggtacacga
    11221 ggaagagact cgccgagatc tggtccgacg agaacaggta cagaaagtgg ctcgacgtcg
    11281 agctcgccgt gtgcgaggcc tgggccgagc tcggcaggat accaccggag agcatggaga
    11341 cgatcaggcg acgggccgcc ttcagcatcg agcgcatcga ggagatcgag gccgtcaccc
    11401 gccacgacgt gatcgcgttc gtgagcgcgg tggccgagca cgtcggagag gacgcccgct
    11461 tcatccacct cggcctcacc tcgtccgacg tcctggacac ggcctacgcc ctgctcctga
    11521 aagagagcgg aatggtcatc gtcgaggaca tccaggacat gcgggccgtc ctcaaagacc
    11581 gcgcctacga gttcaaggac ctcccggcca tcggccgctc ccacggggtg cacgccgagc
    11641 ccatcacctt cggcctgaag ttcgccctgt ggtacgcgga gatgggccgc aacctcgagc
    11701 ggatgatgcg tgccatcgac aggatctcct tcggcaagat ctcgggagcg gtggggacct
    11761 tcgcgaacgt cccgcccgag gtcgaggcgt cggtgtgcgc gcgcctcggg ctcaagccgg
    11821 cgcccatcag cacgcaggtc ctccagcggg accgtcacgc cgagttcttc tgcacgctcg
    11881 cgatcgtggg cgcgagcgcg gagaagatcg cgacggagat caggcatctc cagcgcaccg
    11941 aggtgctgga ggcgaccgag ccgttcgggg cgggccagaa gggttcctca gccatgcccc
    12001 acaagaagaa ccccgtgctc accgagaaca tctgcggcct ggcaaggctc ctgagggggt
    12061 acgccatgag cgcgctcgag aacgtggccc tgtggcacga gcgggacatc agccactcga
    12121 gcgtcgagcg cgtgatcggg cctgacgcca cgaccgtgct cgacttcatg ctcgcgaggc
    12181 tcaccggcgt gctcaggggg ctcgaggtgc acacggacaa tatcaggcgc aacctggagc
    12241 tcaccggcgg gctgtggcac tcgcagaacg tgctcctcgc cctcgtcgac aggggggttg
    12301 cgagggacac cgcctacacg tgggtgcagc gcaacgccct caaggcatgg gagacgaagg
    12361 cggacttcaa ggggctcctc ctccaggatc cggacgtcgc ccgcacgctc gacccacgga
    12421 cgatagacga tctcttcgac ctgtcacacc acctggcgca catagacgcc atcttcgaaa
    12481 gggtcttcaa tgagtgacat cgacgatctc agggacatcg tgaagcgcga cgcggtccgt
    12541 ttcggaaagt tcatcctgtc ctccggcaag gagtcggacc tctacgtaga cctccgcatg
    12601 gtcactctcc acccgagggg ggcctccctc atcggcaggc tcatcttcga ccggatcagg
    12661 gacctcgaga tcgacgcggt cggcggcatg tccataggcg cggacccgat agccgtgtcc
    12721 gtgagccttg cggcattctc ggagggcagg cag
//
LOCUS       DAOSCR010000007        11777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_3662_length_11777_cov_3.491042, whole genome shotgun sequence.
ACCESSION   DAOSCR010000007 DAOSCR010000000
VERSION     DAOSCR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_11777_cov_3.491042"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLT69_00555"
     CDS             <1..296
                     /locus_tag="PLT69_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOJ12971.1"
                     /translation="VCCGEPMRRLEENTVDAAREKHVPVIEKAPGGYVVKVGSVAHPM
                     EERHYIEWIELVADGVALRKFLRPGDEPVARFHTDAESVHAREYCNLHGLWKA"
     gene            313..828
                     /locus_tag="PLT69_00560"
     CDS             313..828
                     /locus_tag="PLT69_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOJ12972.1"
                     /translation="MLSERLEEALNRQINAELYSAYLYLSMEAYFRSVNLEGFARWMR
                     AQALEEMTHADRFAKYISNRQGRVVLDGIARPPVDWDSPRDVFEKVLEHERKVTALIF
                     DLVALAEKEKDYSTGSMLQWFVNEQVEEEATAEGILGQLKLVGDNGNGLLMIDRELGT
                     RVFAPEPPAGR"
     assembly_gap    1484..1493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1494..2609
                     /locus_tag="PLT69_00565"
     CDS             <1494..2609
                     /locus_tag="PLT69_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOJ12973.1"
                     /translation="RGSTYNAYLILADKVTLVDTVKAPFLHEMMARISNLIDPRDIDI
                     IISNHSEMDHSGSLREVVAQVAPEQVIASVMGAKALKEHHLAGDGIVPVKDGQDLSLG
                     NMRVRFMETRMLHWPDSMFTYLEDERILFSQDAFGMHLATGERFADEIDRDILRYEAA
                     TYYANILLPYSGLVLKLLDRVAASGMDVSVIAPDHGPIWRTGIDAVIGWYRAWAQQKP
                     TDKAVVVYGTMWHSTELMARAIEEGLLAGGARVKVMSMEAFHRSDVAYEVLDAGALFA
                     GSSTLNNNMLPAMADVLTYLKGLRPANLVGAAFGSYGWSGEAAGHVQDILAEMKVELV
                     AEPVKSLYVPDDHVLRECFDLGMKAAQIVRQKSGRHG"
     gene            2676..2834
                     /locus_tag="PLT69_00570"
     CDS             2676..2834
                     /locus_tag="PLT69_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOJ12974.1"
                     /translation="MKRYVCGVCGYIYDPEKGDPEGNIPAGTPFEKLPDDWTCPVCGA
                     EKSEFSPE"
     gene            2958..3815
                     /locus_tag="PLT69_00575"
     CDS             2958..3815
                     /locus_tag="PLT69_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOJ12975.1"
                     /translation="MREYPLRSSPPGPELGSLLEEVYGVYHRPEYIHPDPLEFPRMFD
                     DPLDREIAALVSSSLAYGNVRAIRSSLSAVFCRMGSSPRRFVEFSSRGGLEGAFSGFR
                     HRFTGDAELVDFLWALGRVVEGFGSLKACFLEGFNPADPDVFSALGAFTSKIAAFMRA
                     GKNSLLPCPGSPSACKRLNLFLRWMVRHDEIDPGGWPEVPKSSLVVPLDVHMHAIGLR
                     LGLIRRRSADRKAALEMTDALRNIDPNDPVRFDFSLTRIGMRRLLGRLTDAPCGTGRH
                     DEDIPVGGL"
     gene            3817..4314
                     /locus_tag="PLT69_00580"
     CDS             3817..4314
                     /locus_tag="PLT69_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ12976.1"
                     /translation="MDERIKEAIHQAFTGEAKAHLRLKVYAEKAEEEGFPQLAKLFRV
                     IAFSEELHGKRALRLLKEIKSTQDNLAESFESETKVAGVAYDAFVRLAEELGDRAASL
                     YFSQSKDAEEIHAKLYKEAMDHVLEERDTTYYVCTVCGYVSDGVLPEECPICGAKKDV
                     FVTFS"
     gene            complement(4423..5214)
                     /locus_tag="PLT69_00585"
     CDS             complement(4423..5214)
                     /locus_tag="PLT69_00585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOJ12977.1"
                     /translation="MRDINAPANPLEMLAYILASQIHDHKVVYVGTGLPMIAGILAKK
                     THAPNITMVYESGGQDPLPGDMPWSVGCPFTWRKSPVVMEMAYSFAQCYNGYVDIAFL
                     GFAQVDMYGNVNTHQIGKDLLNPTVRMTGSGGNNDLSSLAEEVVLVGLQTPEKFVKEV
                     DFITSVGHLRGGETRKEAGLLGKGPVAVVSQCGVFDFEPVSKRMRVKSLMPGWTFDFA
                     QLFTGFELMRPEGDIPQTEVIPEDILKLLRTEVDPRGVFTTMPTS"
     gene            complement(5228..6316)
                     /locus_tag="PLT69_00590"
     CDS             complement(5228..6316)
                     /locus_tag="PLT69_00590"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOJ12978.1"
                     /translation="MATYQYNQEALANLKIEDPSILDRLIDFESARLVHLKKERAKVD
                     KRMSLKEAIATYVEDGDVMTDAGFAYVRTPHQAYWEIMRQDKKRLQCIGAPNTNHSFL
                     IFNNNVDYSHNSYVGVEMRGIDRNYDRMLRAGRVKILSEWSHGGVALGLKAAQLGTPG
                     VFSKQMLGSDILKYNPYLKVMQNPMRDDPDPVVFIPALYPDVTIIHCQLADKYGNAYI
                     YGPTVNDLATAAAARKVIITAEEIVPESDIRNRQGTVIPFFYVDAVVELPFGAVPGNM
                     PGYYYWSRQWWEKLIRLGCTSDENLKKFFDEWVFGVKDQFEFVDKLGGAKWIAEARIQ
                     TKAAEGDHEDIDFTYEEYTRTHDSGLYY"
     gene            complement(7266..8567)
                     /locus_tag="PLT69_00595"
     CDS             complement(7266..8567)
                     /locus_tag="PLT69_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12979.1"
                     /translation="MGKSAVPFLCVIVVSLGSAPGEGIASQDIIFPPLITVSTEHGRT
                     STSLFGPLVAYDEGFFAVRPLFTRDRTKDVTTFLYPLGKSTPEGSYFVPIMRSGTSDD
                     HRTFDLFPYFSGTYRNRPYSGVFPLYGRMEHRLGFDEAEFFLWPLYSRTTTAGVSTRA
                     VFWPVFSWSDSGMFRVFPLFGRETEKDTTRTFALWPVFHLERSPERLMLAALPLFRYE
                     TGPSHGNLSIVWPLFSYSRDDAAGHTSVDAPWPLVRFASGGYEETRLFPLFWAKEEGP
                     SYRYLSVAWPLWSSSSSAEGEVAKTASSVLILNRYTTTRGPEGVRSRSLTLWPLFHFS
                     RDGTRSRWFFPAIVPLFHDEPFMSSWGEVLTLAKGSSGPEGVEWDILWKTLSVKTSPA
                     YSRWWFSFLVSHEQTLRGRRWGFLGGLIGTPRPKEQAEGVN"
     gene            complement(8571..9596)
                     /locus_tag="PLT69_00600"
     CDS             complement(8571..9596)
                     /locus_tag="PLT69_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOJ12980.1"
                     /translation="MSNEARVGIFVVVVFAVFVVLSMKIGELSFNKKKTYPVTMVFST
                     VEGLKVSSPLELAGVEVGKVSGITLNKDFTAVVRAEINEDVKLPVDSSATIATKGVLG
                     DKIIQITPGVSRSYLEPGGNLARTTVPPSLETLLTQVGELAQNLTELSESLNQSLGDG
                     QAMREIVTNIRDLSEVASTLARENKDDLEAIISNTHRITDEFVTVSENLASSSRDLGE
                     ITRTVNSGQGTLGRLVKDEELYASLVTFTEQASELLEKMNGDSSLGLLLSDTSLYEDL
                     SKTAENLRTITDAMASGKGTIGRLMTDDEIYDEMKDALASANKAMKGIEEQTPITVMG
                     TVLGIVW"
     gene            complement(9593..11077)
                     /locus_tag="PLT69_00605"
     CDS             complement(9593..11077)
                     /locus_tag="PLT69_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ12981.1"
                     /translation="MMLARLEENRGNLVNALYLYARVGDPYAELAKARVYFMMDDSVA
                     ALASLQKVIDEGTYTQEALEQRIRIHARGGNWPLAMQDMLEIARKHPENVQIQMNLAN
                     LCIITQDFERAKTVLKGLIGKADDALVHYTMAKACLGERDFACSKEELNKSIEAKSDF
                     VPAYLDLARIHTLLGETAQAEATYLRLLDVDPLSSEAHLALVDHYIQQKRYREAVDHL
                     ETFYELNPQAQVLRKLVVLELQEGLFEKALAHIRDIKDMTEDDKYYLALTYAGLERYE
                     EALNTLNEISVSGRLGCETAMLASSVLKSMNRDEESIAVLESAWKEYGQTGTCNEIGY
                     QLATELDTAGRRDDGLRIAMELLEKDGKDPIALNFVGYVWADRGVNLDDAYRMIKEAL
                     SAKPDDPYILDSMAWVLHRLGRNEEALGYITRSLEKIGDDPTVNEHMGDILKALGRMD
                     KALDYYLKSSILSRSTTKDLKDKIIDFLRQDRMQGTSTQERSIP"
     assembly_gap    11127..11136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11265..>11777)
                     /locus_tag="PLT69_00610"
     CDS             complement(11265..>11777)
                     /locus_tag="PLT69_00610"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ12982.1"
                     /translation="RSLRKVTDAHLDCHLMIVDPERYIDDFAQSGANGITVHAEATRH
                     LQRTLCSIKERGLVSGVSLNPSTSLDTVAYCLDVIDLLLVMTVNPGFGGQTFIEAMYR
                     KIAQAREMIGTRAIQLQVDGGVTKENIGRLVRLGVDNVVAGSSVFHTDDPATTVREML
                     AIASSSAKHE"
BASE COUNT         2096 a         3581 c         3838 g         2242 t
ORIGIN      
        1 tcgtgtgttg cggtgaaccc atgagacgtc tcgaggagaa caccgtcgat gcggcgcgcg
       61 agaagcatgt cccggtgatc gagaaggccc caggggggta tgtggtgaag gtggggtcgg
      121 ttgcgcaccc catggaggaa aggcactata tcgagtggat agagctcgtg gccgacggcg
      181 tggcgctcag gaagttcctg aggcccggcg atgagcccgt cgctcggttc cacaccgatg
      241 cagagagcgt ccacgcccgg gagtactgca atctccacgg gttgtggaag gcgtgaaagg
      301 gagggtgacg ccatgctcag cgaacggctt gaggaggcat tgaacaggca gatcaacgcg
      361 gaactctact cggcgtacct ctacctgtcc atggaagcgt acttccgctc ggtgaacctc
      421 gaggggttcg cccggtggat gagggcccag gctctcgagg agatgacgca tgcggacagg
      481 ttcgcgaagt acatctcgaa ccggcagggc agggtcgttc tcgacggcat agcccgccct
      541 ccggtggact gggattcccc ccgggatgtc ttcgagaagg tcctcgagca cgagaggaag
      601 gtgaccgcgc tcatcttcga tctcgtggcg ctcgccgaga aagagaagga ctactcgaca
      661 ggctccatgc tccagtggtt cgtgaacgag caggtggagg aagaggcgac cgcagagggg
      721 atcctcgggc agctcaagct cgtgggcgac aacgggaacg gcctgctcat gatcgaccgg
      781 gagctcggca cccgcgtgtt tgcaccggag cccccggcag gccggtaagc ttgcgggcag
      841 gcccttgccc cctgaccgga aacatgaggc gggcatgacc gaggggtccg aggagcactc
      901 ccgtcgtctc tcgtgttccg ggaggggccg ggtacagggc ggcaccccgt gatccccggg
      961 aaaagggtgg cggctctgcc gtctttgggt atcccctcgc gactgagacg aatcggcgcg
     1021 cggtcgaaca tgttcggccg tccacccctg gaggcagagg gaaccagggg gattccgaga
     1081 gcgaggcagg cgtcaaaggc agggccctgt ctcgtcgaac ctgtccgccg aggcctcgcc
     1141 gcaagcgggc acaggggcgg caggtggtgt cgctaccggg gaaaagacat cccatgtatg
     1201 gcgtcatgag gcagggggaa gcccgggagg cagacgggag gccgctacga gatgcgttct
     1261 ctcatacggg aaggcgggtg tgaaaggtgg attaccgtgc gtccttttca tgcatgaagc
     1321 gggcctggat tgacaatgag catccagctg tgggacactc catgagatcg atgcgcgggg
     1381 atccggttcg tctgaacagc cgatgtattc ggggaggtga tcatgactga cacgttcaaa
     1441 cccatacggg taagcgacaa ggtgtactgg gtgggtgccg tcgnnnnnnn nnnaggggga
     1501 gcacctacaa cgcgtacctg atcctcgcgg acaaggtgac cctcgtcgat acggtcaagg
     1561 cgcccttctt gcacgagatg atggcccgca tctcgaacct catcgatccc agggacatcg
     1621 acatcatcat ctccaaccac tcggagatgg accattccgg gtcgctcagg gaggtggtgg
     1681 cccaggtggc tcccgagcag gtgatcgcct cggtcatggg tgccaaggcg ctcaaggagc
     1741 accacctggc aggcgacggg atcgtccctg tcaaggacgg ccaggacttg agcctcggga
     1801 acatgcgggt aaggttcatg gagacgagga tgctccactg gccggacagc atgttcacct
     1861 acctggagga cgagcggatc ctgttctcgc aggatgcctt cggcatgcac ctcgcgaccg
     1921 gggaacggtt cgccgacgag atcgaccgtg acatcctgag atacgaggcg gccacgtact
     1981 atgccaatat cctgctcccc tactcgggcc tcgtcctcaa gctgctcgac cgggttgcgg
     2041 catcgggcat ggacgtctcc gtgatcgcgc ccgaccacgg gcccatctgg cgcacaggca
     2101 tcgatgccgt gatcggctgg taccgtgcgt gggcccagca gaagcccacc gacaaggcgg
     2161 tggtcgtcta tggaaccatg tggcacagca ccgagctcat ggcccgcgcc atcgaggagg
     2221 ggctcctggc gggcggcgcc agggtcaagg tgatgtccat ggaggccttc cacaggagcg
     2281 atgtggccta cgaggttctc gacgcagggg cgctgttcgc cgggtcctcc acgttgaaca
     2341 acaacatgct gcccgccatg gccgacgtgc tcacctacct caagggcctg aggcccgcga
     2401 acctcgtggg agcggcgttc ggctcgtacg ggtggagcgg cgaggccgca ggacatgtac
     2461 aggacatcct cgccgagatg aaggtcgagc tcgtggccga acctgtgaag tccctctacg
     2521 tgcctgacga ccatgtcctg agggagtgct tcgacctggg catgaaggcc gcgcagatcg
     2581 tcaggcaaaa aagcgggcgg cacgggtgac caccgggtcc tgccggggat tcagggtctt
     2641 gtgtaaagga ctgcatgatc agaatggagg gcatgatgaa gagatatgtg tgcggtgtgt
     2701 gcggttacat ctacgacccc gaaaagggcg accccgaggg gaacatcccg gccggaaccc
     2761 ccttcgagaa gctccctgat gactggacgt gccctgtctg cggcgccgaa aagagcgagt
     2821 tctcgcccga gtgacgcctg caccgggtgc ggccgggtga ggcccgtggc gcgtcctcga
     2881 ggtggagccc gccgtcaaga gcggaccaca aggcggtatc cgtgcgtccc cgttgcgtag
     2941 gggccaacaa gggcgtcgtg cgggaatacc ccctgagatc gagccctcct ggacctgagc
     3001 tgggctcgct cctggaagag gtctacgggg tctatcacag gcccgaatac atacaccccg
     3061 atcccctcga gttcccccgc atgttcgacg accccctcga cagggagatt gcggcgctgg
     3121 tgtcgtcctc gctcgcatac gggaatgtca gggcgatccg ttcgagcctt tccgcggtgt
     3181 tctgccggat gggctcctcc ccgcgccgtt tcgtggagtt ttcctcgcgg ggcgggctcg
     3241 agggtgcgtt ttcgggattc aggcatcgct tcacgggcga tgccgagctc gtcgacttcc
     3301 tgtgggccct cggcagggtg gtggaggggt tcgggtcact gaaggcctgt ttcctcgagg
     3361 gcttcaaccc tgccgacccc gacgtgttca gtgcgctcgg cgcgttcacc tcgaagatcg
     3421 ccgctttcat gcgggccggc aagaacagcc tgctcccatg ccccgggtcc ccaagcgcct
     3481 gcaagaggct caacctcttc ctcaggtgga tggtgcggca cgacgagata gaccccggcg
     3541 ggtggccgga ggtccccaaa tcgagcctcg tggtgccttt ggatgtgcac atgcacgcca
     3601 tagggctccg cctgggcctg atccgcaggc gaagcgccga taggaaggcc gcgctcgaga
     3661 tgacggatgc actcaggaac atagacccga acgatccggt ccggttcgat ttctcgctca
     3721 cccgcatcgg catgagaagg cttctgggtc gcctgacgga cgcaccatgc gggacgggcc
     3781 gacatgatga agacatacct gttggagggc tttgacatgg acgagaggat caaggaagcg
     3841 atccatcagg cgttcacggg cgaggcgaag gcccacctga ggctcaaggt ctacgcggag
     3901 aaggccgagg aggaggggtt cccccagctg gcgaagctct tccgcgtgat cgcgttctcg
     3961 gaagagctcc atgggaagag ggccctgagg ctcctcaagg agatcaaatc cacccaggac
     4021 aacctcgcgg agagcttcga gtcggagacg aaggtcgccg gggtggccta tgacgccttc
     4081 gtgaggctgg ccgaagagct tggcgacagg gcggcatcgc tctacttcag ccaatcgaag
     4141 gacgccgaag agatccatgc gaagctctac aaggaggcca tggatcacgt gctcgaggag
     4201 agggacacca cgtactacgt ctgcaccgtg tgcggatacg tgtccgacgg cgtcctaccg
     4261 gaagagtgcc cgatctgcgg cgcgaagaag gacgtgttcg tcacgttctc ctgatcgaga
     4321 aacccccatg ctgccgggcc ggtgagacgc ttgcggcgcg gcatgcatcg ctgcgacgcg
     4381 tcgatgaagg gccggcaggg agacggccgg ccgagggccg ggtcacgacg tgggcatggt
     4441 ggtgaacacg cccctcggat ccacctctgt cctgagcagc ttgaggatgt cctccgggat
     4501 gacctcggtc tgcgggatgt ctccctcggg cctcatgagc tcgaacccgg tgaacagctg
     4561 cgcgaagtcg aaggtccacc cgggcatgag ggatttcacc ctcatgcgct tcgagacggg
     4621 ctcgaagtcg aagaccccgc actgggaaac gacggcgacc gggcccttcc cgagcagacc
     4681 ggcctccttg cgtgtctccc cgcccctgag gtgtcccaca gaggtgatga aatccacctc
     4741 cttgacgaac ttctcgggtg tctggagacc cacgaggacc acctcctcgg cgagcgaaga
     4801 gaggtcgttg ttcccccccg atccggtcat gcgcaccgtc gggttcagga gatccttgcc
     4861 aatctggtgg gtgttcacgt tcccatacat gtccacctgt gcgaagccca gaaaggcgat
     4921 gtccacatac ccgttgtaac actgggcgaa agagtaggcc atctccatga ccaccgggga
     4981 tttcctccag gtgaacgggc accccaccga ccagggcatg tctcccggca gggggtcctg
     5041 gccgcccgat tcgtagacca tggtgatatt gggtgcatgg gtcttcttcg ccaggatccc
     5101 ggcgatcatg gggagcccgg tccccacgta gaccaccttg tgatcgtgga tctggctcgc
     5161 gaggatgtac gcgagcatct cgagcgggtt cgcgggtgcg tttatatctc tcatgtgccc
     5221 tcccgtctca gtagtagagc ccggaatcgt gtgtccgggt gtattcttcg tacgtgaagt
     5281 cgatgtcctc gtggtcgccc tcggccgcct tggtctggat cctcgcctct gcgatccact
     5341 tcgcgccgcc cagcttgtcc acgaactcga actggtcctt gacgccgaac acccactcgt
     5401 cgaagaactt cttgaggttc tcgtcgctgg tgcagccgag cctgatgagc ttttcccacc
     5461 actgtctcga ccagtagtag tagccaggca tgttccccgg gacggcgccg aaaggcagct
     5521 cgaccaccgc atccacgtag aagaagggga tgaccgttcc ctgcctgttc ctgatgtcag
     5581 actcgggcac gatctcctcg gccgtgatga tgaccttgcg ggctgcggcg gccgtggcga
     5641 gatcgttcac cgtcgggccg tagatgtagg cgttcccgta cttgtcggcg agctggcagt
     5701 ggatgatcgt gacgtccggg tacagggccg gtatgaagac caccgggtcg gggtcgtccc
     5761 tcatggggtt ctgcatgacc ttcaggtacg ggttgtactt gaggatgtcc gagccgagca
     5821 tctgcttgct gaagacgccc ggcgtcccca gctgcgcggc cttgagaccc agcgcgaccc
     5881 cgccgtggct ccactcggag aggatcttca cgcggccggc cctcagcatg cggtcgtagt
     5941 tcctgtctat gccccgcatc tccacgccca catacgagtt gtgggagtaa tcgacgttgt
     6001 tgttgaagat gaggaaacta tggttggtgt tgggggcgcc gatgcactgc aggcgcttct
     6061 tgtcctggcg catgatctcc cagtacgcct ggtggggtgt gcgtacgtac gcgaatccgg
     6121 cgtccgtcat cacgtcgccg tcctccacgt acgtggcgat agcctccttg aggctcatgc
     6181 gtttgtccac cttcgcccgt tcctttttca ggtgcacaag gcgtgcggac tcaaagtcga
     6241 tgagcctgtc gagaatgctc ggatcctcta tcttgaggtt tgcgagggcc tcctggttgt
     6301 actgatacgt tgccatcgat gctctccttg cacgaattcg acgcgatggc aggtagttca
     6361 gcaagagacg tgccataggg aagctatcgt ccacacgttg tgtatttatt cgataccacc
     6421 cgggatttcc ccggccaagg gtcttcgagg gggatctcgt gccgcgaagt tcccgggtga
     6481 accaggggtt cttccgccat agtatggtga aaacaccata tcttcgcgac caggcggcct
     6541 cgggctgggt gagggctctg gcaaaaaagg gtgcaaacac cacagccctg tcttcgtgca
     6601 cggcttgcgg caggcacggt gaattcgaga ccgagcccat gatccacggg ccctttgcct
     6661 tcccggctct cgggagccgg accttcgggc ctgaggctcg gcctcttcac ctggccgtgc
     6721 agggtatctc tcgcatgacc tcggtgacga gacctcgcgt gcacggacac cgatcacgct
     6781 cgccagcggc atcgccctct tcctgggacg agacctcgcg tgcacggaca ccgacccggt
     6841 aacgtctgac ggcgcgaccc cgtaggagac ggttcctcgc gtgcgcggac accgagctgc
     6901 gggcgagagg gttacaccct ggctggtcac gacgatccct cgcgtgcacg gacatcgacg
     6961 ccgatccggt gcgcctccgc gtgtcttcgg ggcccgcgtt catgaacggc ctgctgcatg
     7021 ccacgggtgg ggtggcggtc tcgttcttta gccgggaggc gcactgcggg gtgaatgctc
     7081 gacgcgcaca ccgggtatcc ctggccccac gcgtttcccg gaggaagcgg ggatctggag
     7141 agatggatgc tcgacgcgca caccgtggac cccaaagcgg agagagagag gcggctcccc
     7201 atcatggcgc agaccaaggc gcgcgcccga ggggatccgg ccgataccct cgcggcaggg
     7261 gggcctcagt tcacgccttc ggcttgctcc ttagggcgcg gggtccctat gaggccgccc
     7321 aaaaagcccc accgccgacc ccttaaggtc tgctcgtgcg agacgaggaa ggagaaccac
     7381 catctgctgt atgcagggga ggttttcacg gacaacgtct tccacaggat gtcccattcc
     7441 acgccctcag gccccgagga ccccttggcc agggtcaaga cctcccccca cgacgacatg
     7501 aacggctcgt cgtgaaagag cgggacgatc gccgggaaga accagcggct gcgtgtccca
     7561 tcccgagaga agtgaaagag aggccacagg gtgagggatc tcgagcggac cccctcgggc
     7621 cccctggtgg ttgtgtacct gttgagaatg agcaccgacg aggccgtttt ggcaacctcc
     7681 ccttcggcgg acgatgagga cgaccacaag ggccacgcga ctgacaggta cctgtatgag
     7741 gggccctctt ctttggccca gaagagcggg aacaaccgtg tctcctcgta gcctcccgac
     7801 gcgaagcgga ccaggggcca gggtgcgtcg acgctcgtgt ggcctgccgc atcgtcacgg
     7861 ctgtaagaga agagaggcca gacgattgac aggttcccgt gcgaaggccc ggtctcgtac
     7921 ctgaagaggg gaagggcggc cagcatcagt ctttctgggc tcctctccag gtgaaagacg
     7981 ggccagaggg cgaaggtcct cgtggtgtcc ttctcggtct ccctgccgaa gagcgggaac
     8041 accctgaaca tgccagagtc gctccaggag aacacgggcc agaaaacggc gcgggtcgag
     8101 acgcccgccg tggtggtcct cgagtagagg ggccacagga agaactcggc ctcgtcgaag
     8161 ccgagcctgt gctccatgcg gccgtagagc gggaacaccc ccgagtaagg cctgtttcgg
     8221 taggtgcccg agaagtacgg gaagaggtcg aacgtgcgat gatcgtcgct ggtgccgctc
     8281 ctcatgatgg gtacgaaata cgagccctcg ggcgtcgatt tgccgagggg gtagagaaag
     8341 gtcgtgacgt ccttcgtcct gtccctggtg aagagggggc ggacggcaaa gaagccctcg
     8401 tcgtatgcga ccagggggcc gaacaggctc gtcgatgtgc ggccgtgctc tgtcgagacg
     8461 gtgatcaggg gcgggaagat gatgtcctgg gaggcgatcc cctcgcccgg tgcagacccc
     8521 agggagacga cgatcacgca caggaacggt acggcactct tccccatgcc ctaccacacg
     8581 atgccgagaa ccgttcccat gaccgttatc ggtgtctgtt cctcgatccc cttcatggcc
     8641 ttgttcgcgc tcgccagggc gtctttcatc tcgtcgtaga tctcgtcgtc cgtcatgagc
     8701 ctgccgatgg tgcccttgcc cgaggccatc gcgtcagtta tggtacgcag gttttctgcc
     8761 gtcttggaaa gatcctcata cagggaggtg tcgctcagga ggagacccag ggaactgtcg
     8821 ccgttcatct tctccaggag ttcagaggcc tgctcggtga aggtgaccag ggatgcgtag
     8881 agctcctcgt ccttgacaag ccttccgagg gtcccctggc ccgagttcac ggtccgggtg
     8941 atctcgccga gatccctgct cgagctcgcg aggttttccg agacggtcac gaactcgtcc
     9001 gtgatcctgt gcgtgttgga gatgatggcc tccaggtcgt ccttgttttc cctggccagg
     9061 gtggacgcca cctcgctcaa gtcgcggatg ttcgtcacga tctccctcat ggcctggccg
     9121 tctcccaagg actggttcag ggattcgctg agctcggtga ggttctgcgc gagttccccc
     9181 acctgggtga ggagtgtttc cagtgacggc ggcaccgtcg tccttgccag gttgcccccg
     9241 ggttcgaggt aggaacggga cacgcccggc gtgatctgga tgatcttgtc gccgagcacg
     9301 cccttggtgg cgatggtcgc actcgagtcc accgggagct tcacgtcctc gttgatctcc
     9361 gccctcacga ccgccgtgaa atccttgttc aaggtaatgc cgctcacctt ccccacctcc
     9421 acccccgcga gttcgagggg ggatgaaacc ttgagaccct cgacggtgga gaacaccatg
     9481 gtgacgggat aggtcttctt cttgttgaag ctcagctcgc cgatcttcat ggagagcacc
     9541 acgaagacgg cgaagaccac gaccacgaag attcctacac gggcttcgtt gctcacggta
     9601 tcgacctctc ctgggtggat gtgccttgca tcctgtcctg cctgaggaaa tcgatgatct
     9661 tgtccttgag atccttggtc gtcgaacggc tgaggatgga cgatttcagg tagtagtcca
     9721 acgccttgtc catgcggccc aatgccttca ggatgtcgcc catgtgctcg ttgaccgtgg
     9781 ggtcgtcccc gatcttctcg agagacctgg tgatgtagcc taaggcctcc tcgttgcggc
     9841 cgagccggtg gagcacccat gccatggagt cgaggatgta cgggtcgtcg ggctttgccg
     9901 agagggcctc cttgatcatc cggtaggcgt cgtcgaggtt cacgccgcgg tcagcccaga
     9961 cataccccac gaaattgagc gcgatggggt ctttcccgtc cttctccagg agctccatgg
    10021 caatgcgcag cccgtcatcc ctcctccccg ccgtgtcgag ctcggtcgcc agctggtagc
    10081 cgatctcgtt gcacgtgccg gtctggccgt actccttcca ggccgattcc agcacggcga
    10141 tggattcctc gtccctgttc atgctcttca ggacggatga ggcgagcatg gcggtctcgc
    10201 accccagcct ccccgagacg gagatctcgt tgagcgtgtt gagcgcctct tcgtaacgct
    10261 cgagcccggc gtaggtgagc gcgagatagt acttgtcgtc ctcggtcatg tctttgatat
    10321 ccctgatatg ggccagggct ttctcgaaca acccctcctg cagttcgagg acgacgagct
    10381 ttctcagtac ctgcgcctgg gggttgagct cgtagaacgt ctcgaggtgg tcgaccgcct
    10441 cccggtatct cttctgctgg atgtagtggt caaccagggc gaggtgggcc tcgctggaga
    10501 ggggatcgac atcgaggagc ctcaggtagg tggcctccgc ctgcgccgtt tcgcccagca
    10561 gggtgtgtat gcgggccagg tcgagatagg ccgggacgaa gtcggacttg gcttcaatcg
    10621 acttgttcag ctcctccttg gagcatgcga aatcacgttc acccaggcag gccttcgcca
    10681 tggtgtagtg cacgagggcg tcgtcggcct tcccgatcag ccccttgagc accgtcttgg
    10741 cgcgctcgaa gtcttgcgtg atgatgcaca ggttggccag gttcatctgt atctgcacgt
    10801 tctccgggtg cttgcgcgcg atctccagca tgtcctgcat ggccagaggc cagttcccgc
    10861 cgcgtgcgtg tatccgtatc cgctgctcga gagcctcctg ggtgtacgtg ccctcgtcga
    10921 tgaccttctg cagggaggcg agcgcggcga cgctgtcatc catcatgaag tagaccctgg
    10981 ccttggccag ctccgcgtac ggatcaccca cccgcgcata gaggtagagg gcgttcacca
    11041 ggttccccct gttctcctcg agccttgcga gcatcatgag cagccgcggg ttgtaaccca
    11101 gtgcgggccg gtttcaacgt ggggagnnnn nnnnnngccg taccggcacc tggaagtggc
    11161 tgcatgccga caccgtcagc gccgcgagca gcgccgcgag gagacgcacc ctggatgcgg
    11221 tctccctcgg accgaccggg gagcctgccc gtgcaccccg gctttcactc atgttttgca
    11281 ctcgaagagg caatggcgag catctccctt accgttgtgg cggggtcatc ggtgtggaaa
    11341 acgctcgacc ccgcgacgac attgtctaca cccaaccgta cgagcctgcc aatattctcc
    11401 ttcgtgacgc cgccgtccac ctggagctgg atggcccgcg tgccgatcat ctcccttgcc
    11461 tgggcgatct tgcggtacat cgcctctatg aaggtctggc ccccgaaccc ggggttgacg
    11521 gtcatgacga ggagcaggtc gatgacgtcc aggcagtacg ccacggtgtc gaggctcgtc
    11581 gaggggttga gggacacccc cgagacgaga cccctctcct tgatggaaca aagggtgcgc
    11641 tggaggtgcc tcgtggcctc cgcgtgaacg gttatgccgt ttgcgccgct ctgcgcgaag
    11701 tcgtcgatgt accgctcggg gtccacgatc atgaggtggc agtcgaggtg ggcgtcggtc
    11761 acctttctga gggagcg
//
LOCUS       DAOSCR010000008        11451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_3780_length_11451_cov_3.121885, whole genome shotgun sequence.
ACCESSION   DAOSCR010000008 DAOSCR010000000
VERSION     DAOSCR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3780_length_11451_cov_3.121885"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..669)
                     /locus_tag="PLT69_00615"
     CDS             complement(109..669)
                     /locus_tag="PLT69_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOJ12983.1"
                     /translation="MAEEEKDQKKVTQESPKKKFPVLVVVIALCVILVAGGAVGAYFM
                     FFASKKKGEGDHASAEVKQEQVQKKGEAGDAHGGGPGGEGGGSGSVKPLDPFIVNLAD
                     AQGQRYLKAVMQLETDNPSADSEINAKLPQIRDEILMILSNKTFDDVSTVAGKRMLKR
                     EIASAVNRYLSSGQVTNVFFTEFVVQ"
     gene            complement(695..1408)
                     /locus_tag="PLT69_00620"
     CDS             complement(695..1408)
                     /locus_tag="PLT69_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOJ12984.1"
                     /translation="MNTTGTKIVKVRRPDCLGWMTTFSDMVTLLLTFFVMIIAMSSLD
                     SKALKETFGYFRWAAGVLEFPDSQELRVHKDEVQRERVVETSLQALSDGVDRALREQA
                     AGLAGKGKDLVEVVEDRRGLAVRIKGDVLFPEGASTISEDARPVLMAVASVLKETSGT
                     ISVEGHTDAVGGEMDNRYLSLERASRVADFLVMEAGLKASRFCVAGYGMTKPVATNET
                     PSGRRKNRRVEIVLLRNTL"
     gene            complement(1405..2199)
                     /locus_tag="PLT69_00625"
     CDS             complement(1405..2199)
                     /locus_tag="PLT69_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ12985.1"
                     /translation="MARRKIVYEDPPVNSGMFLYCSLILIMLTFFVVLASMSVQDSRR
                     QRLAIGSILGSFGVMPGGRSPFSSKDVRSLSPLSPPMDERPVSIEIIEDAIQATGRYR
                     DIKVTRGRLGVTVTLPAAILFEDGTDRISPGSLEVLGAVANLLKRMDNEILVVGHTDS
                     IPVDRPPYGSNWGLSGARALSVLSYFREKGVRPERMTAYGMGSTRPVADNETEEGRRT
                     NSRVEIVMVGELPGDVAMEEKKGEAPERRPRSFFYRGYRIDLEDRR"
     gene            complement(2203..2982)
                     /locus_tag="PLT69_00630"
     CDS             complement(2203..2982)
                     /locus_tag="PLT69_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOJ12986.1"
                     /translation="MDLATVIGVLGAFGLVIVGISAGGPLTLFVNVPSLLITVGGTFG
                     FIFINYPLKDVLGLFGVVRNVFMAPRRDVRSLIPTFVDFATKARREGILALESAAEEL
                     RDPFFRHGLELVIDGLEPQSIREILETEIEHIEHRHTRGADILNSMGTFAPAMGMVGT
                     LIGLVQMLQSMSDPSTIGPAMAVAIITTFYGAIMANLVALPMAGKLTMRSREEVLEKG
                     MILEGIMAISAGDNPRIVERKLHSFLSPSMREQVVARSRED"
     gene            complement(3252..4763)
                     /locus_tag="PLT69_00635"
     CDS             complement(3252..4763)
                     /locus_tag="PLT69_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOJ12987.1"
                     /translation="MSISSSLFSGISGLSTHGNAMSVIGDNIANVNTIGFKSSRTTFQ
                     DVLAQSVATSAGTAQIGRGAALSAIDTLFQQGSFESTSNATDLAIGGNGFFIVSPRGS
                     ETEYYTRAGQFRFDADGYFVNPEGYVVKGWALDENGNDVGTLQDIRLSAFTSPPEATN
                     TVTAITNLDSQETSHSASLFGAWNANDPTGTVIGDANYGYQTSIRVYDALGNSHDITI
                     IYDKLNSAASGNEYDDGSVDIENSWEYIVTCNPADDLRPDFETADARGILMRGTLTYD
                     AASGSLRSMSAFVHAGGDPSDPANWVPATSFSPDGYPEFTAQFVSGVDQTMAVRFGAR
                     STDNTWTGAASIADIATDGLPTSSWEPQALTSTQYASSSTTVYQTQDGYGTGFLESIS
                     VSTDGVMVGHYSNGQILNLYRVGLAKFNNQQALFKVGGNLWSATRESGDAITGHPGEN
                     GLGRISPNSLEQSNVDIAAEFVKMITTQRGFQANSRIITTVDDMLQELINLKR"
     gene            complement(4787..5458)
                     /locus_tag="PLT69_00640"
     CDS             complement(4787..5458)
                     /locus_tag="PLT69_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOJ12988.1"
                     /translation="MAVGLDNILAVQSAADNASAGTSITKVDEQKNMFLKLLVKQLQY
                     QDPLNPLENTEFATQLAQFSMLESMNTMSENITLMTQFQNSMNSMEAASFIGKTVNAS
                     GNVISFTGAETAVDFALADNASEVRLTIYNESGTPVRTMTMNDVAKGDVSWRWDGRND
                     RGETVSPGRYYFTVGATGYDGSAVNTSTYANGVVTGVRFDNGTIYLQVGDKEVRLADI
                     TRISG"
     gene            complement(5481..7091)
                     /locus_tag="PLT69_00645"
     CDS             complement(5481..7091)
                     /locus_tag="PLT69_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOJ12989.1"
                     /translation="MDAPVGALQKAYCPSAGQGPFEPWLEEASSAPGLSGTGPQAPGL
                     GLTREDKGGVTQVTPQSAVIYLFALGSMGLSPNDIRAVVCGDPSGLSDDGLMHILETS
                     GYDPALVRQVMDDAGLCAMLKESCVGALSSMLTEGGAGEGMGPHSPDAGWGSNQLGPV
                     RPQVGAHGLLLVEAESEQASQGNTVDGAASSNFTGGAPARRVTEPLVQVLAYSVRKAL
                     EGCPGLQPEQPGVSSNVDGLGSGASPSGAQADGVGEDPSTPGPALEELVGGTRVLEET
                     FGVENDTLRDLVFSAEPSARRSALTEAISRMTRGLDEGGRDGLQAGLKKALSVLKTVL
                     SKDEYSELVASLKAAGHEVPAGVAGVPVNKGLFEAFAHRLAHVPEGALQPQLEGALDQ
                     VRQAIFTLPRAGEGSVSIRIHPPMLGRVDIDVFLEGGQVTATFKVDQPLTRDLIQQNM
                     QVLKETLAECGIKAQQLVVTSEANTWADRQGPLVWIGSDGPGGKSNGQGRSNEGKGQW
                     QERTHAPVTYDVASPGTYVEGTGLDLIA"
     gene            complement(7554..8675)
                     /locus_tag="PLT69_00650"
     CDS             complement(7554..8675)
                     /locus_tag="PLT69_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ12990.1"
                     /translation="MTKSSREFGLILGAHTLFFLNFSELILLPVYFTDTGYSPGRIGA
                     FMAAFSVAVVASLPVCGLLSDRLPRRRMFALGALLMAVPSALYGALPRPDLAIPILRA
                     LQGIGFSSAFGIMGAMVADLDSHEDTRTRLGVLTAAGMATHAAGPALGEFLVARWGYQ
                     ALFFSSASFGLASLAVSFFLPGKTPPRAEAFPAGRSLIRPAWASAVLGAVFGGTTVFV
                     PPLVDARVQGGAALFFVSLVAGSLAVWAALSRHRNTSGWAPPRAAWIAASVLTTLLPI
                     LLSRTGRPAGYLILGTLFGAGYGYLYPSLNSVMIETSRNRAGSANAFFVWSFNIGMLV
                     STLGLGMAIESQGIEAPMRILGLSCLVLLLWARVKRTPR"
     gene            complement(8656..9315)
                     /locus_tag="PLT69_00655"
     CDS             complement(8656..9315)
                     /locus_tag="PLT69_00655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ12991.1"
                     /translation="MGRPGVIVSFDLDGTLTDHAFVDGVWNEGLPELVSRRLHVPLDE
                     ARDMCARAYAREGDDSVAWYRLPYWLAFFGIEDVRAEDLIERYTGRIRAFEDGLRSLA
                     RLSDMGVGLVLFSNATRAFLDRELEVTGLKRFFGEVVSLPDDWGTLKACPDAFVRLGS
                     LAGGVLVHVGDHVRYDYEVPMSVGVRAYHVWRGTGRRLQCSMETLDTLVEALGRDKVI
                     P"
     gene            complement(9297..10925)
                     /locus_tag="PLT69_00660"
     CDS             complement(9297..10925)
                     /locus_tag="PLT69_00660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ12992.1"
                     /translation="MAPSEDLLGRFGPSIEEVRRAVDAMPSPDGAASAYSFPLSLRSP
                     TGEDIGLSCTVINLDRCRVLFLEEPRSAQRPLFPLEDRDIFVRMFTNSPVAAIAVDKG
                     LIAYANDRFSRIMDMGRDALLGRELSGFVSRHSLNTLLGASKRWCADDGAPPEPVEVM
                     FVTPSGRRVRMEVRGGCTERRGRKVVWMVMTDLSRVESLERRLAEERQRYEELFDKMP
                     IGILYVDPRGRIVECNRAVSTMTGFPAERIRGFLFTEFVDDEQVDELRDAFGQLFAHG
                     HEITRREGTIKTAGGRTITIEYDARPIYRKGRCTMALMVFTDVTAKKALERELIEKNE
                     EMERTLWDMAEVKDALEARAGELNRATEELKLLNERLNLLSITDGLTELYNHRHFQDR
                     LTEEMERLNRAKDGSLSLLMIDIDDFKSFNDTYGHQCGDMVLKHISFLIKSTIRSIDI
                     VARYGGEEFAVILPNATKEQALLVAERICQVIRSTPFSYGGASNAKVTVSIGVGTVDK
                     GVTDKSMLVARADEALYSAKAKWKDRVEVWEDPG"
     gene            complement(10982..>11451)
                     /locus_tag="PLT69_00665"
     CDS             complement(10982..>11451)
                     /locus_tag="PLT69_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ12993.1"
                     /translation="MGHWHDDATLEDAPRIVFPMPEVDLSDKRVLVVDDVSDEGCTME
                     GVVAYLRGKVESIRTAVVVSKAQPRLRADYSAMTIDEWRWVFFPWSLHEDLLSFTEQV
                     LNATGGATAEDIVRILGESMSVDIARHEIERVLADMQRSGEVKEDGNRVWTLI"
BASE COUNT         1903 a         3837 c         3568 g         2143 t
ORIGIN      
        1 ctcgtcctcg gcccgcacgt ccgtctcggt ggggatgtcc ccgccgatca cgcctttcaa
       61 gagggcatca acttcttcct gggtcaggat ctgtcccatg tggactcctc actgcacgac
      121 gaattccgtg aagaagacgt tggtcacctg ccccgagctc aggtaacggt tgaccgcgct
      181 cgcgatctcg cgcttgagca tgcgcttgcc cgcgacggtg gacacgtcgt cgaatgtctt
      241 gttggagagg atcatcagga tctcgtcgcg gatctgcggc agctttgcgt tgatctcgct
      301 gtcggcagac gggttgtcgg tctcgagctg catgaccgcc ttgaggtagc gctgtccctg
      361 ggcgtccgcc aggttcacga tgaaggggtc gagcggcttg acagagcctg agccgccgcc
      421 ttcaccgccg gggcccccgc catgggcatc ccctgcctcc cccttcttct ggacctgttc
      481 ctgcttgacc tccgccgacg cgtggtcgcc ctcgcctttc ttcttggaag cgaagaacat
      541 gaagtaagcc ccgaccgccc cgcccgcgac gaggatcacg cacagcgcaa tgacgacgac
      601 gagcaccggg aacttcttct tggggctttc ctgcgtcact ttcttctggt ccttttcctc
      661 ttcagccatg gaatcctcca tcgtctttca ggtgttacaa ggtgtttctc agaaggacga
      721 tctccaccct cctgtttttc cgcctgccgc ttggggtttc attcgtggcc acgggcttcg
      781 tcatgccgta tccagccacg cagaacctcg acgccttgag gcccgcctcc atcaccaaga
      841 aatcggccac gcgcgacgcc ctctcgagcg agaggtaccg gttgtccatc tcgccgccga
      901 cagcgtccgt gtgaccctcg acggagatcg tcccgctcgt ctccttcagc acggacgcga
      961 ccgccatgag caccgggcgg gcgtcctctg agatcgtcga ggccccctcg gggaagagga
     1021 cgtcgccctt gatccgcacg gccagccctc tccggtcctc caccacctcc acaaggtcct
     1081 tgcctttgcc ggcaagcccc gcggcctgtt ccctcagggc cctgtcgacc ccatcggaaa
     1141 gcgcctgcag ggaggtctcc accacgcgct ccctctgcac ctcatcctta tggacacgaa
     1201 gctcctggga gtctggaaac tcgaggacgc ctgcggccca cctgaaatac ccgaaggttt
     1261 ctttgagggc cttgctgtcc agggagctca tggcgatgat catgacgaag aaggtgagca
     1321 ggagcgtgac catgtccgag aacgtggtca tccaccccaa gcagtcgggc cgcctcacct
     1381 tcacgatctt cgtacccgtc gtgttcatcg cctatcttcc aggtctatcc tgtaacccct
     1441 gtagaagaag gatctgggcc ttcgttcggg agcctcccct ttcttctcct ccatcgccac
     1501 atcccccggg agctccccga ccatgacgat ctccaccctg ctgttggtcc tcctcccctc
     1561 ttccgtctcg ttgtcggcca cgggtctcgt cgatcccatg ccgtaggctg tcatccgctc
     1621 gggcctcacg cccttctcgc ggaaatagga gaggacggag agggccctcg cccccgagag
     1681 accccagttc gacccgtacg gcggcctgtc gacggggatg gaatccgtgt ggcccacgac
     1741 gaggatctcg ttgtccatcc gcttcagaag attcgccacc gcgccgagga cctcgaggct
     1801 gcccggcgat atcctgtcgg tgccgtcctc gaacaggatg gctgcaggga gggtcacggt
     1861 caccccgagc ctgcccctgg tcacctttat gtcacggtac ctgccggtgg cctggatggc
     1921 gtcctcgatg atctcgatgc tcaccggtcg ttcgtccatg gggggtgaaa gggggctcaa
     1981 cgacctcacg tccttggagg agaacgggct cctcccgccg ggcatgacgc cgaaactccc
     2041 caggatcgac cctatggcga gcctctgtct cctgctgtcc tggacgctca ttgaggcgag
     2101 aaccacgaag aaggtgagca tgatgaggat gagggagcag tagaggaaca tgcccgagtt
     2161 caccggaggg tcctcgtaga cgatcttcct gcgggccatg gatcagtcct ccctggaccg
     2221 tgcgacgacc tgctcgcgca tggacggcga gaggaacgag tggagcttgc gctcgacgat
     2281 cctcgggttg tcgcccgccg agatggccat gatgccctcg aggatcatcc ccttctcgag
     2341 gacctcttcc ctggaccgca tggtgagctt gcccgccatg ggcaacgcca ccaggttcgc
     2401 catgatggcc ccgtagaacg tcgtgatgat ggccaccgcc atcgcgggcc cgatggtgga
     2461 gggatcggac atcgactgga gcatctgcac gaggccgatc aaggtcccca ccatgcccat
     2521 ggcgggggcg aatgtcccca tggagttcag gatgtcggca cccctggtgt gcctgtgctc
     2581 gatatgctcg atctcggtct cgaggatctc cctgatcgac tggggctcga gcccgtctat
     2641 gacgagttcg aggccgtgcc tgaagaacgg gtccctcagc tcttccgccg cgctctcgag
     2701 cgccaggatc ccctccctgc gcgccttcgt cgcgaagtct acgaaggtcg ggatcaggct
     2761 gcggacgtcc cgcctgggcg ccatgaagac gtttcgcacc acgccgaaga ggccgagcac
     2821 gtccttgagg gggtaattga tgaagatgaa cccgaacgtg ccccccaccg tgatgagcag
     2881 gctcggtacg ttgacgaaca gggtgagcgg gcctcctgcc gatatcccca cgatgacgag
     2941 cccgaaagcc cccagtacgc ctatgactgt tgcaagatcc atggacctcc ccttgccttc
     3001 tcgatcgtcc tctctgggct cgatgaaagc aaggggtatg ccatgagcct cccttctttt
     3061 atctcattga aatgccgtat cttttcgatg atcaggatgg gcaaggagcc gggcccggcc
     3121 agtcactatt ttgacgcatc tggcctgccg gccctgcacg ttacgctgta tccgggcggc
     3181 ccggatccct gcctcaaggt tcggaggcgg gaggacccgg gccgcctgtc agcacgtggc
     3241 gtcaggggtc atcagcgctt caggttgatg agctcctgga gcatgtcgtc cacggtcgtg
     3301 atgatcctcg agttggcctg gaagcccctc tgcgtggtga tcatcttcac gaactcggcg
     3361 gctatgtcca cgttcgactg ctcgagggag ttgggggaga tcctccccag gccgttctcg
     3421 ccggggtggc cggtgatggc gtcaccggac tccctggtgg ccgaccagag gttgcccccc
     3481 accttgaaca gggcctgctg gttgttgaac ttggcaagcc ccacccggta gaggttcagg
     3541 atctggccgt tgctgtagtg acccaccatg accccgtccg tgctcaccga gatgctctcg
     3601 aggaaacccg tgccgtagcc gtcctgggtc tggtacaccg tcgtcgagga cgaggcgtac
     3661 tgggtggagg tcagggcctg gggctcccag gaagaagtcg gcaggccgtc ggttgcgatg
     3721 tcggcgatcg acgccgcccc ggtccacgtg ttgtccgtgc tcctggcgcc gaacctgacc
     3781 gccatggtct ggtccacgcc cgagacgaac tgggccgtga actcggggta gccgtccggc
     3841 gagaaggacg tcgcgggcac ccagttggcc gggtccgacg ggtctccgcc cgcgtgcacg
     3901 aacgcgctca tggaacggag ggaaccgctc gccgcgtcgt aggtgagcgt gccgcgcatg
     3961 aggatgcccc tggcgtcggc ggtctcgaag tcgggcctga gatcgtcagc cgggttgcac
     4021 gtcacgatgt actcccagga gttctcgatg tccacggacc cgtcgtcgta ctcgttcccg
     4081 ctggccgcgg aattgagctt gtcgtagatg atggtgatgt cgtgggagtt gccgagggcg
     4141 tcgtagacgc ggatcgaggt ctggtagccg tagttcgcgt caccgatgac ggtgcccgtg
     4201 gggtcgttcg cgttccatgc cccgaacagg ctcgcggagt gagatgtctc ctggctgtcg
     4261 aggttcgtga tggcggttac ggtgttggtg gcctccggcg gcgaggtgaa ggcgctgagc
     4321 cggatgtcct ggagcgtgcc gacgtcgttg ccgttctcgt cgagcgccca ccccttgacg
     4381 acatacccct cggggttcac gaagtagccg tctgcgtcga acctgaactg cccggccctc
     4441 gtgtagtact cggtctcgct ccctctcggg gacacgatga agaagccgtt gccgccgatg
     4501 gcgaggtcgg tggcgttgga cgtggactcg aaggacccct gctggaagag ggtgtcgatg
     4561 gcgctcagcg ccgcgccgcg gccgatctgg gcggtgccgg ccgaggtcgc gaccgactgc
     4621 gcgagcacgt cctggaacgt ggtcctgctc gacttgaacc cgatggtgtt gacgttcgcg
     4681 atgttgtccc cgatcacgct catggcgttc ccgtgcgtgg acagcccgct gatcccggaa
     4741 aacagtgaac tcgatatgct catgtgatcc tccttccctt cgtgctctat ccggatatcc
     4801 tggtgatatc cgcaagcctg acctccttgt cgccgacctg cagatagatc gtgccgttgt
     4861 cgaacctcac gccggtcacg acgccgttgg cgtacgtgct ggtgttcacc gcagaaccgt
     4921 cgtagccggt cgcgcccacg gtgaagtaat acctgcccgg gctcacggtc tcgcccctgt
     4981 cgttcctgcc gtcccacctc cacgagacgt cgcccttggc cacgtcgttc atggtcatgg
     5041 tcctcacggg ggtccccgac tcgttgtaga tggtcagcct cacctccgag gcgttgtccg
     5101 ccagggcgaa gtcgacggcg gtctccgctc ccgtgaacga tatgacgttg ccggacgcat
     5161 tcacggtctt gccgatgaag gacgcggcct ccatgctgtt catggagttc tggaactgcg
     5221 tcatgagcgt gatgttctcg ctcatggtgt tcatggactc gagcatggag aactgggcga
     5281 gctgtgtcgc gaactcggtg ttctcgagcg ggttgagcgg gtcctggtac tggagctgct
     5341 tgacgagcag cttgaggaac atgttcttct gttcgtcgac cttcgtgatc gacgttcccg
     5401 cggatgcgtt gtccgcagcc gactgcaccg caagtatgtt gtcgagacct actgccatgt
     5461 cttcctcctt aggccgtccc tcaggcgatg agatccaggc cggtcccctc cacgtacgtg
     5521 ccgggggacg cgacgtcgta ggtgacgggc gcatgggtgc gctcctgcca ctgtcccttt
     5581 ccctcgttcg acctgccctg gccgttcgac ttgccgcccg ggccgtccga gccgatccac
     5641 accaaaggcc cctgcctgtc ggcccacgtg ttcgcctccg aggtcaccac gagctgctgg
     5701 gccttgatgc cgcactccgc gagcgtttct ttcaggacct gcatgttctg ctggatgagg
     5761 tcgcgggtga ggggttggtc gaccttgaac gtcgccgtca cctggccgcc ctcgagaaag
     5821 acgtcgatgt ccaccctgcc cagcatgggc gggtggatcc tgatggagac agacccctca
     5881 cctgctcgcg gcaaggtgaa gatggcctgc ctcacctggt ccaatgcccc ctcgagttgg
     5941 ggctggaggg cgccttcagg cacgtgggcg agccggtggg cgaaggcctc gaacaaaccc
     6001 ttgttcaccg ggacccccgc gacacctgcg ggcacctcgt gccctgcggc cttgagcgag
     6061 gcgaccagct ccgagtactc gtccttggac aagaccgtct tgagaacact cagcgccttc
     6121 ttgagaccgg cctggaggcc gtctcgaccc ccctcatcga ggccccgggt catgcgcgag
     6181 atcgcctccg tcagggcgga cctcctcgcg gaaggctccg ccgagaacac gaggtctctc
     6241 agcgtgtcgt tctccacccc gaacgtctct tcgagcaccc tcgtcccgcc cacgagttcc
     6301 tcgagggcag gcccgggggt cgacgggtct tcaccgacac cgtcagcctg tgcacccgac
     6361 ggcgatgcac ccgacccaag accgtccacg ttcgacgaaa cgcccggctg ctccggctgc
     6421 agccccgggc agccctcgag cgcctttctc acggagtatg cgagcacctg caccaggggc
     6481 tcggtcaccc tgcgtgcagg cgcacccccc gtgaagttcg aagacgccgc accatcgacg
     6541 gtgtttccct gagaggcctg ctccgactcc gcctcgacga gcaggagacc gtgggcaccc
     6601 acctgtggtc gcacgggtcc gagctggttc gagccccatc cggcgtccgg ggagtggggc
     6661 cccattcctt ctcccgcccc accttctgtg agcatcgaag acagcgcccc cacgcaagac
     6721 tccttgagca tggcgcacaa gccggcgtcg tccatcacct gtctcaccag cgccgggtcg
     6781 taacccgagg tctcgaggat gtgcatcagg ccgtcgtcgc tcaggcccga ggggtctcca
     6841 cacaccacgg cgcgaatgtc gtttgggctc aaccccatgg aaccgagggc gaagaggtag
     6901 atgacagccg attgcggggt gacctgcgtg acaccgccct tgtcctcgcg tgtcagcccc
     6961 aggccgggag cctgcggccc cgtcccggag agaccggggg ccgaggacgc ctcctccagc
     7021 cagggctcga aaggcccctg tcccgctgaa gggcaatacg ccttctgcaa ggcgccgacc
     7081 ggtgcatcca tgcatcccat gagtacaggt aggggagatg gcatgttctc ctccgctctc
     7141 ttgtcgtttc cccctgcaga gcaatggcgg tgccaagggg cgcgaacgtg catgccggcc
     7201 gcttcatggg ccgcaccacg ggcccgggag ggaaattatg cacacacctc ccgggcaaat
     7261 cttgccggga gggccctgtt gtcaccaaga cgccccccga cccacagggc ccctcgagcc
     7321 tggctgcctt ctccttgaaa ggccggacat ggggcggaga gtgtcgatga taggacagga
     7381 caaggtcgcg gcgcgtgacg gatcctcgca gcacgagagg gcatgtcagg ctcgcacgcc
     7441 ttgtaccacc cgaggacgcc ggtcactgcc ccacgggccc gggcggacac ccctcgccgt
     7501 cgagccgggc cttgccgggc gggggttgtg ccggcgcgcc cacgcacggg gcctcaccgt
     7561 ggcgtccgct tcacacgggc ccacagcagg aggacgaggc aggacaggcc gaggatcctc
     7621 atcggcgcct cgattccttg ggactctatg gccatcccga gccccaaggt ggagacgagc
     7681 atgccgatgt tgaaggacca gacgaagaag gcgttggcag agcccgcccg gttgcgggag
     7741 gtctcgatca tgaccgagtt caggctgggg tagaggtacc cgtaacccgc accgaacagg
     7801 gtgccgagga tgaggtagcc ggcagggcga ccggtcctgc tcaagaggat gggcaagagc
     7861 gtcgtcagga ccgatgccgc gatccaggcg gccctcggcg gagcccatcc cgaagtattc
     7921 ctgtgccgcg agagggccgc ccacaccgcc aaggagccgg ccacaaggga aacgaagaag
     7981 agcgccgccc ccccctgcac gcgggcatcc accagcgggg ggacgaacac ggtggtgccg
     8041 ccgaagaccg cgcccaagac cgcgctcgcc catgccggcc tgatcaggga ccggccggcc
     8101 gggaaggcct cggcccgtgg cggggtcttt ccaggaagga aaaaggagac agccagggaa
     8161 gccagcccga acgatgccga gctgaaaaag agcgcctggt agccccagcg ggccaccagg
     8221 aactccccca aggcagggcc tgccgcatgg gtcgccatcc ccgccgcggt gagaacgccc
     8281 aggcgtgttc gcgtatcctc gtgcgagtcg aggtccgcga ccatcgcgcc catgatgccg
     8341 aaggcgctcg agaagccgat gccttggagg gccctgagta tcgggatcgc caggtccggc
     8401 cggggcagtg cgccgtacag cgcgctcggc acggccatga gcagggcgcc gagggcgaac
     8461 atgcgcctcc tggggagacg gtccgacagc aggccgcaca ccggcaggct cgctaccacc
     8521 gccacgctga acgcggccat gaacgcgcct atgcggcccg ggctgtagcc ggtgtccgta
     8581 aagtacacgg gcaggaggat gagctcggag aaattcagga agaagagggt gtgggccccg
     8641 aggatgaggc cgaattcacg ggatgacttt gtcacggccc agggcctcga ccaaggtgtc
     8701 gagtgtctcc atcgagcact gaagcctcct gcccgtgcct ctccacacgt ggtacgcccg
     8761 caccccgacg ctcatgggca cctcgtaatc gtaccggacg tggtcgccca cgtggaccag
     8821 gaccccgcct gcgaggctcc cgaggcgcac gaatgcatcg gggcatgcct tgagcgtgcc
     8881 ccagtcgtca ggcagcgaga cgacctcgcc gaagaagcgc ttgagtcccg tcacctcgag
     8941 ctccctgtcg agaaaggccc tggtcgcatt ggagaacagg acgagcccca cccccatgtc
     9001 actcaggcgc gcaagagacc tcaaaccgtc ctcgaaggca cggatcctcc cggtataacg
     9061 ctcgatgaga tcttcagccc ggacgtcctc gataccgaag aatgcgagcc agtaagggag
     9121 cctgtaccag gcgaccgagt cgtcaccttc ccgggcgtag gccctcgcgc acatgtccct
     9181 ggcctcgtcg agggggacgt ggagcctcct ggagaccagt tcaggcaggc cctcgttcca
     9241 caccccgtcg acaaacgcat ggtccgtgag ggtcccgtcg aggtcgaagg agacgatcac
     9301 cccgggtctt cccatacctc gaccctgtcc ttccacttcg ccttcgccga gtacagcgcc
     9361 tcgtccgccc gcgccacgag catggacttg tccgtgaccc ccttgtccac ggtccccacc
     9421 cctatgctca ccgtcacctt cgcgttcgag gcccctccgt agctgaacgg cgtcgaccgt
     9481 atcacctggc aaatgcgctc tgccaccaag agggcctgtt ccttggtggc gttcggcagg
     9541 atgacggcga actcctctcc gccgtaccgg gcgacgatgt cgatgctccg gatggtgctc
     9601 tttatgagga aggagatgtg cttgagcacc atgtcaccgc actggtggcc gtaggtgtcg
     9661 ttgaagctct tgaaatcgtc tatgtcgatc atgagaagcg acagcgagcc gtccttcgcg
     9721 cggttgaggc gttccatctc ctcggtgagg cggtcctgga aatggcggtg gttgtagagc
     9781 tcggtgaggc cgtctgtgat ggacaggagg ttcagtctct cgttgaggag cttgagctcc
     9841 tcggtcgcac ggttgagctc gccggccctt gcctcgagtg cgtccttgac ctccgccatg
     9901 tcccacaggg tgcgttccat ctcctcgttc ttctctatga gctctctctc cagggccttc
     9961 ttggcggtga cgtcggtgaa gaccatgagg gccatggtgc acctgccctt gcggtatatc
    10021 ggcctggcgt cgtactcgat ggtgatcgtc ctgccgcctg cggtctttat cgtgccctcc
    10081 cgcctggtta tctcgtgccc gtgggcgaag agctggccga aggcatcgcg gagctcgtcc
    10141 acctgctcgt cgtccacgaa ctccgtgaac agaaagcccc ttatcctctc ggccggaaac
    10201 ccggtcatgg tggatacggc cctgttgcac tccacgatcc tgcccctggg gtccacgtac
    10261 aggatgccga tgggcatctt gtcgaacagt tcttcgtacc gctgccgttc ttccgcgaga
    10321 cgcctctcga gcgactcgac cctgctgagg tcggtcatca ccatccatac gaccttcctc
    10381 ccgcggcgct cggtgcaccc acccctcacc tccatgcgga cccgcctgcc tgagggggtc
    10441 acgaacatca cttccacggg ctcgggggga gcgccgtcgt cggcgcacca cctcttggac
    10501 gcgccgagaa gggtgttgag gctgtgcctc gacacgaacc ccgagagttc cctgccgagc
    10561 agggcatccc ggcccatgtc catgatcctc gagaagcgat cgttggcgta tgcgatgagg
    10621 cctttgtcca ccgcgatcgc ggcgacaggg ctgttcgtga acatgcggac gaagatgtcg
    10681 cggtcctcga gggggaaaag gggtctttgt gccgagcggg gctcttccag gaagagcacc
    10741 cggcatcggt ccaggtttat cacggtgcag gaaaggccga tatcctcccc ggtaggcgag
    10801 cggaggctca aggggaagga gtaggcgctc gccgccccat cgggagaagg catggcgtct
    10861 accgccctcc tcacctcctc gatggacgga ccgaaccttc ccagcaaatc ctcgctcggt
    10921 gccaaggcgc ccccctcttc gaagaccgca acatagggcg acagaagcgc ttgcaagaac
    10981 cctatatcaa ggtccatacc ctgttgccgt cctcttttac ctcgcccgag cgctgcatgt
    11041 cagcgaggac cctctcgatc tcgtgcctcg ctatgtccac ggacatcgat tcgccaagga
    11101 tcctcacgat gtcctcggcc gtggcgccgc ccgtggcgtt gaggacctgc tccgtgaacg
    11161 agaggagatc ctcgtggaga gaccacggga agaacaccca cctccactcg tcgatggtca
    11221 tggcgctgta gtccgccctg agccggggtt gggccttgct cacgacgacc gccgtcctga
    11281 tgctctccac cttccccctc aggtaggcca ccaccccttc catggtgcac ccctcgtcgg
    11341 acacgtcgtc gaccacgagc acccgcttgt cggagagatc gacctcgggc atggggaaga
    11401 cgatccgggg tgcgtcctcg agggtggcat cgtcgtgcca gtgacccatc t
//
LOCUS       DAOSCR010000009        11273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_3843_length_11273_cov_3.426457, whole genome shotgun sequence.
ACCESSION   DAOSCR010000009 DAOSCR010000000
VERSION     DAOSCR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_11273_cov_3.426457"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLT69_00670"
     CDS             complement(<1..429)
                     /locus_tag="PLT69_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ12994.1"
                     /translation="MGRYIELYRDTWAMMDSFMQLFPDLGPAHPARMDGHGSGPILSD
                     VLVKVIEKDREAIVKAWADEVKSSIPALAPAIDEIERLNRAVLEGVRLGLRGQRHPFD
                     RDMLRREGRSLREKGLPLPEILDALALSRKNIWVHVIGKRI"
     gene            complement(414..902)
                     /locus_tag="PLT69_00675"
     CDS             complement(414..902)
                     /locus_tag="PLT69_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ12995.1"
                     /translation="MRRSRAYCAYCGASLVRRDIEGRERDGCPACGAVFYENPLPVAS
                     AIVVDDDRRILLVRRNNEPYRGMWCLPIGFAEAGEEVKDAALRELEEETGVRGTVVRL
                     IDVDTVENDYYGSLAIVTYEVRATGGRLRPGDEPRTPPISPSSISRRWRGRPTRRRWA
                     GT"
     gene            complement(899..1765)
                     /locus_tag="PLT69_00680"
     CDS             complement(899..1765)
                     /locus_tag="PLT69_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ12996.1"
                     /translation="MHQLFSEEEIRKTYEELLWHKRSRDIILEYALNRSDVREEALRG
                     LDLGGAREVIDLGCGYGFFTEALGGRLHRKARVIGVDVVNHRNREAFVDTVTCMGYEA
                     AFIQGSADIVADLETARFDLAIASYSLYFFPHLVPEISRILKDTGVFIAVTHSETSLG
                     EIISHVVRSMEEHGIAPAGDLRINKLFKAFSVEGGRQLLEPHFHQVECIPFANALAFP
                     LDRFEACIDYLDTKKHLLLKEVADHRPEALEAVMDALVRNLFDHARSHGKVVVSKDDG
                     IFRCRRPVRRGP"
     gene            1869..2879
                     /locus_tag="PLT69_00685"
     CDS             1869..2879
                     /locus_tag="PLT69_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ12997.1"
                     /translation="MAEQDVYRELAKRLMMENSETVREIWRIVCSEEEAAIIARMPAT
                     VDELCEAFGKGREEMQAVIDGLFARGAVFDYVKDGVTMYRGPRHIVQFHDATILWPDA
                     PPELFDLWRSFSETDYPGLLELVTSLKLPSFMRVIPIGETVEARNQVLAFEDAAAMLG
                     SAREIAVTTCVCRKLMGRCDAPLEVCLQLDRGAEYTIKRGTGRRVDVGEALEILARAR
                     DAGLVHMTENTAGRSNVLCNCCSCCCEMLRFATDTRTKGVVAPSRFRPRVDAGTCSSC
                     GACAEVCPVGAMGVPEGGTAVVDEAACIGCGLCANSCPLDAIQLVEVRPREFIPGADR
                     AQ"
     gene            complement(3394..7212)
                     /locus_tag="PLT69_00690"
     CDS             complement(3394..7212)
                     /locus_tag="PLT69_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ12998.1"
                     /translation="MLAALFSSNPTAMAVSELPTGKIVEVNEAFVSLTLFAREELVGR
                     EVTGLGIWADPQERERYLELLRTEGAAREFRCRLRRKDGELRDVVLNGSMVRGHRSGG
                     IVVSFLRDVTDQLRLQKEIAQSEERYRRITSAVTDYIYTVHIEGGNVVRTVHGAACEA
                     VTGYTPEDFERDPFLWYTMIFEDDREAVREHFNRVTTGERCQPIEHRIRRKDGTVRWV
                     SNIPVIHRDASGNIVGYDGVLSDITERKLAEKELKESEERFKALHEASFGGIGIHDRG
                     LILDCNRGLEVITGYSREELIGMNGLMLIAPSWREHVMEKIVSGYERPYDVEGIRKDG
                     TIYPLEIQGKQIPYHGKTVRVTEFRDITWRKRIEDEIRVRQARIDSIFRAAPVGIGMV
                     VDRVIQEVNDRVCEMTGYSREELTGVSARVLYPDDDEFERVGREKYAQLEKAGVGTVE
                     TRWKRKDGSIIHIILSSAPIDPRDPGTGYTFTALDITMRKLAEDRLKESEERYRLMAE
                     LTGKLVYDVDLDAGRHTWDGAVEVISGYTREEFASVDMAGWTRMIHPEDRPGVIMAFE
                     RALNTRKPFRAEYRILRKDDKTIYVEDNGVFVTDKSGRPYRMLGSIGDITARRLSELA
                     LKESEERYRNMFDNSHAVMLLVDPASGAIVDANPAACSYYGYSKNELVSMKITDINTL
                     SPREIFAEMERARSSRKRYFNFKHRLADGVVRDVEVFSGPITVEGRTLLFSIVHDVTD
                     RRLAEEALRRNEERFRQILQNIADIIVILDSQGRITYETPSLARVLGYAPGHLVGASP
                     LDLVHPEDKELVARDLTGVYEGTNNGIPTEFRILKADGTWIYLEAIGSRLEGESRQGS
                     VIITARDVTDRKKAEGAKMEMERRMLHAQRLESLGVLAGGIAHDFNNLLMAILGNIDL
                     ARIDVPPGSRAMTFLDNAMMASRRAADLTNQMLAYSGRGRSEFEEIELSGFIQGMTDL
                     IRASISKGAVLTVRAAEGLPPVKADPDQIRQVVMNLVVNASEAIGDAPGSITVSTDLV
                     YCSEGDLSLSRLPDKPAAGEYVVLEVRDTGCGMDENTLELLFDPFFSTKFTGRGLGMA
                     AVSGIVKAHKGAITVESEPGKGTTFRVYLPVAPGKGEPRGGASERGGATDGDTAREGM
                     AGPSEGFLVLVVDDEDLVRETCASMISRLGHRVLTASDGMQALEIYKEKRDKIACVVL
                     DLTMPVMDGIETLAALRRMDEGVKVILSSGYDVAEAGRMFEGLGATGFIKKPYSMEQL
                     RRVLEGVKPD"
     gene            complement(7724..10687)
                     /locus_tag="PLT69_00695"
     CDS             complement(7724..10687)
                     /locus_tag="PLT69_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ12999.1"
                     /translation="MNRLLGRMSEFIVRQRVAVLAVTVAVTCVMVFMIATRITVNNDH
                     DTWLPKHNEVAKLLRQADEDFSSNVMLFAVIEFKDGVFKPENLAVVDRITKELEKMDE
                     LFNVTSVTNIIDIKKTQDGIEVSDLVRSVPKDGGEALGLRDYVMSKETYVNTVVSADE
                     RFTCVMTNIKSDLDEVKVAEKIYRTIDTIAGGHVRYYGGDPSVNYYLNYYMVEDLKKL
                     LPLGVIVVMTVIFLGLRRVSGVVLPMTIVAFAIVWTFGLQAIFSFPVNILSPAVVVLL
                     IALGTDYAVHYYNHHMKRGDVRTSTAEISLPVTMSAFTTIVSLLTFATTRIEVFKNFG
                     LELAFGLAAAWILSIVLMAVFIAIFKVNPQQGADDEGPRQTTFTRATDSLGAFVQRHA
                     KAILVVVGLASVGMAFGVPRISTNVDFIGQLPKDSPPRRGCDILQEHFSGMYPFNLYV
                     RADIQDPAVMNRMQYLENCMRTENGASGFTSINALIAQENWLLNGVYAIPETREGIAN
                     LWFMLEGHDVLKTFVTEGRDKALVNSIVNESSTEDMRFVADRLDAILQGRLSEEIVTV
                     DPSRLTEEGRGALEKVMIRDAAEQVAWLARYYDKKTVVDPLMVEEALTKALHGIEGLV
                     DVEAVRRDVEAFLKTETVEILPEALVAGIMERVGGLVGAGLTREAADDIEGMIVASGA
                     MGAEDAKTTVEGMSRRLDSSLRIQKAVALAKALTSLIPEGIRGNENFVKRTEGVLWGL
                     FSPRPVMFSRQVESVPGISGAIAGSVRAKIDQAGMPATVRLVHHLLISSQVQSLVLAI
                     CAVLVMVSLAHRSLRRGLASMLTVLVPLVFILGFMGLVGMPLDIGTVLCGSLIVGLGI
                     DGSIHFLHYYSRLHEQGTPKDTAIRMTMGHVGKAVCTANGTTLAGFLVCLISDVTAVR
                     NFATLNSIAIVFVTASVMTLLPALVTILHLDRMDDVEEGSRTGTTTVFRLRRPACKGM
                     EKGEEERKAARAAGG"
     gene            complement(11066..>11273)
                     /locus_tag="PLT69_00700"
     CDS             complement(11066..>11273)
                     /locus_tag="PLT69_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13000.1"
                     /translation="LYSGHVSGIPYATHDLHAYTEKVDGEERTIALARVCVTSLDLGG
                     TSSRGSFLMRIANVREIALEGLTP"
BASE COUNT         1825 a         3815 c         3469 g         2164 t
ORIGIN      
        1 gatacgcttc ccgatcacgt gcacccagat gttcttcctg ctgagcgcca gggcgtcgag
       61 gatctcgggc aggggcagcc ccttctccct gagggacctg ccctcccgcc tgagcatgtc
      121 ccggtcgaag gggtgtctct gccccctgag ccccaagcgg accccctcga gcaccgctcg
      181 gttgagcctc tcgatctcgt cgatggcggg cgcgagtgcg gggatcgagg atttgacctc
      241 gtcggcccag gccttgacga tcgcctcgcg gtccttctcg atcaccttca ccagcacgtc
      301 cgacaggatg ggcccgctgc catgcccgtc catcctcgcg gggtgagcgg gcccgaggtc
      361 agggaagagc tgcatgaacg agtccatcat ggcccaggtg tccctgtaca actctatgta
      421 ccggcccacc gccttctcgt tggacgacca cgccagcggc gggagatcga agatggggaa
      481 ataggcggcg tccgaggctc gtcccccggc ctgagcctcc cgcccgtggc cctgacctcg
      541 taggtgacga tggcgaggct cccgtagtag tcgttctcga ccgtgtccac gtcgatgagc
      601 ctcacgaccg tccctctcac gcccgtctcc tcctcgagct cccggagggc tgcgtccttg
      661 acctcctccc cggcctccgc gaacccgatg ggaaggcacc acatgcccct gtagggctcg
      721 ttgttcctcc tgacgaggag gatccgccgg tcatcgtcca ccacgatggc gctcgccacg
      781 ggcagggggt tctcgtagaa caccgcgccg catgccgggc acccgtccct ctccctcccc
      841 tcgatgtcgc gcctcacgag agaggcgccg caatacgcgc agtacgccct gcttctcctc
      901 atggccccct cctcacaggc ctccggcacc tgaagatgcc gtcgtccttg ctcacgacga
      961 cctttccgtg agatctcgcg tggtcgaaca ggttcctcac gagcgcgtcc atcaccgcct
     1021 cgagcgcctc cggcctatgg tccgccacct ccttgaggag gaggtgcttc ttggtgtcga
     1081 ggtagtcgat gcaggcctcg aagcggtcca ggggaaacgc cagcgcgttg gcgaagggga
     1141 tacactccac ctgatggaag tggggctcga ggagttgacg ccccccctca acggagaagg
     1201 ccttgaagag tttgttgatc ctgagatcgc ctgccggggc tatcccgtgt tcttccatcg
     1261 accgcaccac gtgggagatg atctcgccga gagaggtctc gctgtgggtc acggcgatga
     1321 agacccccgt gtccttgagg atgcgcgaga tctcgggcac gaggtggggg aagaagtaga
     1381 gcgaatagct cgctatcgca agatcgaacc tggccgtctc gaggtcagcc acgatgtcgg
     1441 cgctgccctg gatgaacgcc gcctcgtacc ccatgcaggt caccgtgtcg acgaaggcct
     1501 ctcggttgcg gtgattcacc acgtcgacac ctatgacgcg ggccttcctg tggagcctcc
     1561 ccccgagtgc ctcggtgaag aacccgtagc cgcacccgag gtcgatcacc tccctggcac
     1621 cgcccagatc gagccccctc agcgcctcct ccctcacgtc ggacctgttc agagcgtact
     1681 cgaggatgat gtccctgctc ctcttgtgcc agaggagctc ctcgtaggtc ttcctgatct
     1741 cttcttccga gaagagctgg tgcatgcctt ccgtccctcc gacaacccgc tcgatgcatg
     1801 tcataccata cgacagcctt gcatggactt catcggcagg gctatgatga aactataagg
     1861 aggtgggcat ggccgaacaa gacgtgtaca gggaactcgc taaaaggctc atgatggaga
     1921 actccgagac ggtgcgcgag atctggcgta tcgtgtgctc cgaggaagag gcggcgatca
     1981 tcgcccgtat gcccgcgacc gtggacgagc tctgcgaggc gttcgggaag ggcagggaag
     2041 agatgcaggc cgtcatcgac ggtctcttcg ccaggggcgc cgtcttcgac tacgtcaagg
     2101 acggggtcac gatgtaccgg gggccccgcc acatcgtcca gttccacgac gccacgatcc
     2161 tgtggcctga cgccccccct gagctgttcg acctctggcg ctccttctcg gagaccgact
     2221 atcccggcct cctggaactc gtcaccagcc tcaagctgcc gtccttcatg cgggtgatcc
     2281 ccatcggcga gaccgtcgag gcgcgcaacc aggtgctcgc tttcgaggac gcggcggcca
     2341 tgctggggtc cgcccgggag atagcggtga ccacctgcgt gtgccgcaag ctcatgggga
     2401 ggtgcgacgc gcccctcgag gtgtgcctgc agctggaccg cggggcggaa tacaccatca
     2461 agcggggcac gggcaggcgc gtggacgtcg gggaggccct cgagatcctc gcgcgcgccc
     2521 gggacgccgg cctcgtccac atgaccgaga acacggccgg caggtcgaac gtcctttgca
     2581 actgctgcag ctgctgctgc gagatgctca ggttcgcgac cgacacaagg acgaagggcg
     2641 tggtggcgcc gagcagattc aggccccggg tggacgccgg gacgtgctcg tcgtgcgggg
     2701 catgcgccga ggtctgcccc gtgggcgcca tgggtgtgcc ggaagggggg acggccgtcg
     2761 tggatgaggc cgcgtgcatc ggctgcggcc tgtgcgcgaa ctcgtgccct ctggatgcca
     2821 ttcagctcgt cgaggtgagg cccagggagt tcatacccgg agcggaccgc gcccagtgac
     2881 atggcgccgt ggactgctcc ggcaggcagc ctcgggtccc tcccggcgcg cagtcaagac
     2941 gcttgggcct aggcaaacga agagccttct ccacgaggga ctcttgcacc ggcgtccacc
     3001 acggtcgggt ctccctcggg gaggtcgtcc ggaagcgggg gttcctgggg atacaatctc
     3061 ttcgacgagt tcatcggtct tggtctggcc gtcgaggtcg cgcggaagca ggggtccctc
     3121 gggatccctc gcgccctcag acacgcgagg ggggcatcgc cggcctgcgt ccccgcaggc
     3181 tccgctgccg agggagcaca aagcgccagg gcggggaagc gggcctctcc aggaggccgg
     3241 cacctgcggg gcctgcacgc ctcaggcgcc ctccggcagg gcttcgaccc ctgccgaggg
     3301 catcatccgc ccttcgcatg ccctgcggat gcaaggttca ccggtttcca ccccgcagga
     3361 ggcccctcgc cgtcatagtg tcacgacacg ggctcagtcc ggcttcaccc cctcgagcac
     3421 ccgcctgagc tgttccatgc tatacggctt cttgatgaaa ccggtcgccc ctaggccctc
     3481 gaacatcctg cccgcctccg cgacgtcgta gccgctcgac aggatgacct tcaccccctc
     3541 gtccatgcgc ctgagtgcgg cgagggtctc tatgccgtcc atgaccggca tggtgaggtc
     3601 gagcacgacg caggcgatct tgtcacgctt ctccttgtag atctcgaggg cctgcatgcc
     3661 gtctgatgcc gtgagcaccc ggtggccgag ccgggagatc atggaggcgc aggtttccct
     3721 caccaggtcc tcgtcgtcca ccacgagcac gagaaagccc tccgaggggc ctgccatgcc
     3781 ctcacgggca gtatccccgt ccgtcgcccc gcccctttcc gacgcccccc ctctgggctc
     3841 cccctttccg ggggccacag gcaggtacac cctgaaggtg gtacccttgc ccggctcgga
     3901 ctccaccgtg atcgcgccct tgtgtgcctt cacgatgccg ctcacagcgg ccatgccgag
     3961 gccccttccc gtgaacttgg tggaaaagaa ggggtcgaac aagagctcca gggtgttctc
     4021 gtccatgccg cagccggtgt cgcggacctc caacaccacg tattcccctg cagcaggctt
     4081 gtcaggaagg cggctcaggc tcaggtcgcc ctcggagcag tacacgaggt ccgttgaaac
     4141 ggttatggag cccggtgcat caccgatggc ctcggaggcg ttcacgacga ggttcatgac
     4201 gacctgccgg atctggtccg ggtcggcctt gacggggggg agtccctcgg cggccctcac
     4261 cgtgaggact gcccccttgg agatggacgc cctgatgagg tccgtcatcc cctgaatgaa
     4321 cccgctcagt tcgatctcct cgaactccga cctgcccctg cccgagtagg cgagcatctg
     4381 gttcgtgagg tccgcggccc tcctggacgc catcatggcg ttgtccagga aggtcatcgc
     4441 cctggacccg ggggggacgt ctatccgtgc caggtcgata ttccccagga tggccatgag
     4501 gaggttgttg aagtcgtgtg ctatgccgcc ggccagcacg ccgaggctct cgagcctctg
     4561 cgcgtggagc atccgcctct ccatctccat cttcgcaccc tcggccttct tgcggtcggt
     4621 cacgtcgcgc gccgtgatga tcacgctgcc ctgccggctc tcaccctcga gcctgctgcc
     4681 tattgcctcc aggtagatcc acgtcccgtc cgctttcaat atgcggaact cggtggggat
     4741 gccgttgttc gtcccctcgt agacccctgt gagatcgcgg gccacgagtt ccttgtcttc
     4801 gggatggacg aggtcgaggg gggatgcgcc cacgagatgg cccggcgcgt aaccgagcac
     4861 gcgcgccagg gaaggggtct cgtaggtgat gcggccctgg ctgtcgagga tcacgatgat
     4921 gtccgctatg ttctgaagga tctgccggaa ccgttcctcg ttccgcctga gggcctcttc
     4981 ggcgagcctc cggtcggtca cgtcgtgaac gatggagaac aggagggttc ggccctcgac
     5041 cgtgatgggc ccgctgaaga cctccacgtc ccgaacgact ccgtccgcaa ggcggtgctt
     5101 gaagttgaag taccgcttcc tgctggagcg cgcccgctcc atctcggcga agatctccct
     5161 tgggctgagc gtgtttatgt ccgtgatctt catcgagacg agctcgtttt tggaataccc
     5221 gtagtacgag cacgcagcag ggttcgcgtc cacgatggcg ccgctcgccg ggtctacaag
     5281 gagcatgacg gcgtgcgagt tgtcgaacat gttgcggtag cgctcctcgc tctccttgag
     5341 cgccagctcg gagagcctgc gggccgtgat gtcccctatg ctcccgagca tgcggtaggg
     5401 cctcccggac ttgtcggtca cgaacacgcc gttgtcctcg acgtatatgg tcttgtcgtc
     5461 tttcctgagg atgcggtact cggccctgaa gggcttgcgc gtgttgagcg ccctctcgaa
     5521 cgccatgatc actccgggcc tgtcctcggg gtggatcatg cgcgtccagc ccgccatgtc
     5581 caccgatgcg aactcctccc tcgtgtaccc ggagatcacc tccacggcgc cgtcccaggt
     5641 gtgcctgccg gcgtcgaggt cgacgtcgta gaccagcttt ccggtgagct cggccatgag
     5701 gcggtatcgc tcctcgctct ccttgagcct gtcttcggcg agcttccgca tggtgatgtc
     5761 gagggcggtg aaggtgtacc ctgtgccggg atctcggggg tcaatgggcg cagagctgag
     5821 gatgatgtgg atgatggacc cgtccttcct cttccacctc gtctcgacgg ttcccacccc
     5881 ggccttttcc agctgggcgt acttctccct gcccacgcgc tcgaactcgt catcgtccgg
     5941 gtacaggacg cgagcagaga ccccggtgag ttcctccctg ctgtacccgg tcatctcgca
     6001 tacgcggtcg ttcacctcct gtatcaccct gtcgaccacc atcccgatac cgacgggcgc
     6061 agccctgaag atgctgtcga tcctggcctg gcgcaccctg atctcgtcct cgatccgctt
     6121 tcgccaggtg atgtccctga actccgtgac cctcaccgtc ttcccgtggt aggggatctg
     6181 cttcccctgg atctcgagcg ggtagatcgt gccgtccttg cggatcccct ccacgtcgta
     6241 gggcctctcg tagcccgaga cgatcttctc catgacgtgc tcacgccagg agggggcgat
     6301 gagcatgagc ccgttcatgc ctatgagctc ctcgcggctg taacccgtga tgacctcgag
     6361 gcccctgttg cagtcgagga tgaggcccct gtcatggatc ccgatgcccc cgaaggatgc
     6421 ctcgtgcagg gccttgaacc gctcctcgct ctctttgagc tccttctccg cgagcttgcg
     6481 ctcggtgatg tcgctcaaga caccgtcgta gcccacgatg ttcccggatg cgtccctgtg
     6541 gatgacgggt atgttgctca cccacctgac cgtgccgtcc ttgcgcctta tgcggtgctc
     6601 gatgggctgg cagcgctctc cggtcgtcac cctgttgaag tgttccctca cggcctccct
     6661 gtcatcctcg aagatcatgg tgtaccacag gaacgggtct cgctcgaagt cctcgggagt
     6721 gtacccggtg accgcctcgc aggccgcgcc gtgcaccgtg cgcaccacgt tgccgccctc
     6781 gatgtgcacc gtgtagatgt aatccgtgac cgcggaggtg atgcgacggt atcgctcctc
     6841 gctctgtgcg atttccttct ggagcctgag ctggtcggtc acgtccctga ggaagctcac
     6901 cacgatccct ccggagcgat ggccgcggac catggagccg ttgaggacca catctcgcag
     6961 ctcgccgtcc ttgcgcctga gcctgcacct gaattcccgt gccgcgccct cggtgcgcag
     7021 gagttccagg tagcgctccc tctcctgtgg gtccgcccag atgccgaggc ctgtaacctc
     7081 gcggccgacg agttcttccc tggcgaagag ggtcaggctc acgaaggcct cgttcacctc
     7141 gacgatcttt cccgttggca gttcggacac cgccatggcg gtggggttcg acgagaagag
     7201 ggctgcgagc acacgtgagg tcaggggccc ggacctatgg cttttcgacg caacacgggg
     7261 accgtccgta ccgggcgccc cccggccctt ccttcttgca tccatatccc ttcctctccc
     7321 cctggggcac gtcgtactcg gcgtccctgg aagaaaagaa taagacgttc tttcttcctg
     7381 tcaatgaggc gccgggcgcg gtcacccgtc gtccgtgcac ggacgctccg gccgcacccg
     7441 agcccgcagg tcacccctgc atccgccctt cgtggagaca gcttaggccc tcgatcgttg
     7501 accgccttgc tcgtgggcca ccccgcatgc tgcaacgact cccgtcatcg ggcatcgaga
     7561 acaagcgctt tggcctcggg tcgcccatcg ccttcggcca tcgacgggat gagccgcggg
     7621 aacaagagtc cggtcttcca aggccgcacc cccgtccccc ctggcctgcg gtccccgtga
     7681 agatccgcga gagaaaaggg cggttcacct cccgcatctc ccctcatccg cctgccgccc
     7741 gggcggcctt tcgttcctct tcccctttct ccatcccttt gcaggcgggc ctcctgagcc
     7801 tgaacaccgt cgtggtcccg gtcctcgagc cctcctccac gtcgtccatc ctgtccaggt
     7861 gaaggatggt gacgagcgcc ggcaggaggg tcatgaccga tgcggtgacg aacacgatgg
     7921 cgatcgagtt caacgtggcg aagttcctca ccgcggtcac gtccgagatc aggcacacga
     7981 gaaaaccggc cagcgtggtc ccgttcgccg tgcacaccgc cttgcccacg tgccccatgg
     8041 tcatgcgtat ggcggtgtcc ttgggcgtgc cctgctcgtg gagcctgctg tagtagtgca
     8101 ggaaatggat cgacccgtcg atcccgagcc ccacgatgag tgacccacag agcacggtgc
     8161 cgatgtcgag gggcatgccc acgagcccca tgaacccgag gatgaacacg agcggcacga
     8221 ggaccgtcag catggacgcc agcccgcgcc tcagcgagcg atgggccagg gagaccatga
     8281 cgagcaccgc gcagatggcc aggaccaagc tctggacctg gcttgagatg aggaggtggt
     8341 gtacgagtct caccgtcgcg ggcatccccg cctggtcgat cttcgccctc accgagcctg
     8401 ctatcgcccc ggagatgccc ggtacagact ccacctgcct cgagaacatc accggacggg
     8461 ggctgaagag gccccagagg acgccctccg tcctcttcac gaagttctcg ttcccgcgga
     8521 tcccctcggg tatcaggctg gtcagcgcct tggcgagcgc caccgccttc tggatcctca
     8581 ggctcgagtc gaggcgtctc gacataccct cgaccgtggt cttcgcgtct tccgcaccca
     8641 tggcgccgga cgcgacgatc atgccctcga tgtcgtcggc cgcttcgcgg gtaaggcccg
     8701 cgcccacgag tccgccgacc ctctccatga tcccggcgac cagggcctcc ggcaggatct
     8761 ccacggtctc tgtcttgagg aaggcctcga cgtccctcct cacggcctcg acatccacga
     8821 gcccttcgat accgtgcagg gccttcgtga gggcctcctc caccatgagc gggtcaacga
     8881 cggtcttctt gtcgtaatac ctggcgagcc acgcgacctg ctcggccgca tccctgatca
     8941 tgaccttctc cagcgcgcct cgcccctctt cggtgagcct ggacgggtcc accgtgacga
     9001 tctcctcgga caagcggcct tgcaggatgg catccaggcg gtcggccacg aagcgcatgt
     9061 cctcggtgga cgactcgttc acgatggagt tcaccagggc cttgtccctg ccctcggtga
     9121 cgaaggtttt gaggacgtcg tgaccctcga gcatgaacca caggttcgct atcccctccc
     9181 tcgtctcggg gatggcgtac acaccgttca gcagccagtt ctcctgcgct atgagggcgt
     9241 tgatggaggt gaaacccgag gcgccattct cggtacgcat gcagttttcc aggtattgca
     9301 tgcggttcat gacggcgggg tcttggatgt cggcgcgcac gtagaggttg aacgggtaca
     9361 tgccgctgaa gtgctcctgc aggatgtcac atcccctcct cggcggactg tccttgggga
     9421 gctggccgat gaagtcgacg ttcgtgctta tcctcggcac gccgaacgcc atgccgacgc
     9481 tcgccaggcc gaccaccacc aggatggcct tcgcgtgcct ctgcacgaag gcccccaggc
     9541 tgtcggtcgc ccgcgtgaag gtcgtctgcc tggggccttc atcgtcagcg ccctgctggg
     9601 ggttgacctt gaagatcgca atgaacacgg ccatgagcac gatggagagg atccacgccg
     9661 cggccaggcc gaaggcgagc tcgagcccga agttcttgaa gacctctatg cgcgtggtgg
     9721 cgaaggtgag gagactcacg atggtggtga acgcgctcat ggtcacgggc agcgatatct
     9781 ccgccgtgga ggtgcgcacg tcgccgcgct tcatgtggtg gttgtagtag tgtaccgcgt
     9841 agtcggtccc gagcgcgatc agcaggacga cgaccgcagg gctcaggatg ttcacgggga
     9901 aggagaagat cgcctggagg ccgaaggtcc acacgatggc gaacgcgacg atggtcatgg
     9961 ggagcacgac gcccgacacc ctcctcaggc ccaggaagat cacggtcatg accacgatca
    10021 cgccgagcgg caacagcttc ttcaggtcct cgaccatgta gtaattcagg tagtagttca
    10081 ccgatgggtc gccgccgtaa tagcgcacgt gcccgccggc gatggtgtcg atggtccggt
    10141 agatcttctc cgccaccttg acctcgtcca ggtcgctctt gatgttggtc atgacgcagg
    10201 tgaaccgttc gtccgcggat accacggtgt tcacgtaggt ctccttggac atcacgtaat
    10261 ccctcagccc cagcgcctcc ccgccgtctt tcggcacgct cctgacgagg tcggacacct
    10321 cgatgccgtc ttgcgtcttc ttgatgtcga tgatgttggt cacgctggtg acgttgaaga
    10381 gctcgtccat cttctcgagc tccttcgtga tgcggtccac gaccgccagg ttctcgggct
    10441 tgaacacccc gtccttgaac tcgatcacgg cgaacagcat cacgttcgac gagaaatcct
    10501 cgtccgcctg gcgcaacagc ttcgcgacct cgttgtgctt cggcagccag gtgtcatggt
    10561 cgttgttcac ggtgatcctg gtcgctatca tgaagaccat gacgcaggtg accgccaccg
    10621 tcacggcgag gaccgcgacc ctctgcctga cgatgaattc gctcatcctt ccgagaagcc
    10681 tgttcattgg tgaacccccc gcttcattcc atagtatgcc gcatctccgc ccacgcacgc
    10741 aggcgatcgt tcagacacac ccgggtaccg agcccatcgg gatacaccgt agtcccccca
    10801 ccttcggccg agccagacgg catgcaggcg tcaatgtatt atagcaagca tcaatgtgaa
    10861 atgaaatctc atttcttgac cgtggacgga aacgttgtcg ccgccgaggt cctttcgggc
    10921 tgcctgaaca tgtgaaaatt gccgggggaa aagcaggttc aagccctgcc gggaactgga
    10981 cggcgcatgc gccactgcat gaacccatcg agcgcgtcca ccatgaaaac aaggtggtgg
    11041 gcgggcaacg acgacacggg ccgcgtcagg gcgtgagacc ttcaagcgcg atttcccgca
    11101 cgttcgcgat gcgcatgagg aacgaccccc tggaggaggt cccgccgaga tcgaggctcg
    11161 tcacgcacac ccgcgcgagc gctatggtcc tctcctcgcc gtcgaccttt tccgtgtacg
    11221 cgtggaggtc gtgggtggcg taggggatgc ccgagacgtg cccgctgtag agc
//
LOCUS       DAOSCR010000010        10870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4013_length_10870_cov_3.588904, whole genome shotgun sequence.
ACCESSION   DAOSCR010000010 DAOSCR010000000
VERSION     DAOSCR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_10870_cov_3.588904"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLT69_00705"
     CDS             <1..211
                     /locus_tag="PLT69_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13001.1"
                     /translation="HLVTIGCGCHNRRLIEEQYGDTIEFLDSYRQLPKALEHLFRRVF
                     VYGAKPGKPARAALDAARETLPDKG"
     gene            224..634
                     /locus_tag="PLT69_00710"
     CDS             224..634
                     /locus_tag="PLT69_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13002.1"
                     /translation="MELSRQVREYLEQEGKSVFIASSDAQGRVNVAMFGSVRLAPDGM
                     VELMLGDNRTWSNLKENGSAACMVTMHGTSGLSTKGCRLYLRLAEAKDEGADVEAFRE
                     RLAARIGKAADMMRHLVRFEVVEARPIIDLGQGV"
     gene            713..1513
                     /locus_tag="PLT69_00715"
     CDS             713..1513
                     /locus_tag="PLT69_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOJ13003.1"
                     /translation="MNDQTVLLTEVRSNIGTITLNRPHSKNALSIDLLVEIHRTLTAW
                     SRSDDIRCVIVTGGTSRAFSSGYDISSIPTMMTPEIEEVMHRGNPLEFALDAVFSYPY
                     PTIAMMNGYAFGAGLNLALCCDMRIAASDVKVGMPPARLGLVYHPDGLRRFIEVVGMA
                     RTREIFFTGNTYEGEEALAIGLVDRLVPREELEDTVYAVAETIAGNAPLALKGTKRIL
                     SMISERTRLGEEEMNEARRLMREAFESDDLKEGQVAFFEKRPPRFSGK"
     gene            1601..3172
                     /locus_tag="PLT69_00720"
     CDS             1601..3172
                     /locus_tag="PLT69_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ13004.1"
                     /translation="MAIEWNIGFIPYKRAQLTPDRTALVYEDQPVTYRELNQRVNKAA
                     HMLEKKGIKKGDRVALLLLNCVEFLEVYFALAKLGAILVPLNWRLAGPELEYQLNNCD
                     VRMLVFHDVFLGSVDIIRERIKVEQDKFLFLESGSKGLPGLEPPKCPFWAQDYLSLVE
                     PESTDEPEPCEPVTLDDPLAILYTSGVTGSPKGAVVSHLQTFFKNFQVGLYIDASQDD
                     IVIAQMPLFHSGGLFIVATPALCAGMTLVMRRGFDPNEFAEDIQRYRGTIVFALTTMW
                     RMILETGKLDSIDVNSVRRVVGGGERTPPSLFDELAKRNLFMQQGFGQTENSAMMLLP
                     REDIKRKMGSIGKPGFFTEVWIDDGKGRRLPPGEIGEICAKGPTVMSGYWNMPEETAK
                     AVTDGGVLHTGDLGYMDEEGYFYIVDRVKDMYRSGGENVYPAEVEKILSTHPKISMVA
                     IIGVPDDRWGETGMAFVVPKNGEKITENEVLDYLKDKVARYKYPSKVMFMDELPLTAT
                     MKVKKAELKARYGRV"
     gene            3176..4138
                     /locus_tag="PLT69_00725"
     CDS             3176..4138
                     /locus_tag="PLT69_00725"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOJ13005.1"
                     /translation="MTTRGFSNRVNELLGSRYPIVAGPMRLISLGGMAASVSEAGGFG
                     VIAASGLNAKELEREVSLARSLTDRPFGVNIPLYRENAREVIDAALAMGIGVIYTSAG
                     DPQRMVERIKSRGAKVIHKVSSLNMARKAAGAGVDAVVAMGCEAGGHIGKDLVTMFCL
                     VRSITRTLDIPVIASGGIGDGRGLLAALILGAEGVEIGTRLVATRECPVPAFFKEALC
                     SAECTSTLVLGRETMPIRVLANAMALRAAGMASGSLDVEMAARGDDLYAPRAREKDSA
                     IMPCGQVAGLVDGVLEVREVIDSMVSQARAMARGIGESEGWWLA"
     gene            4228..4925
                     /locus_tag="PLT69_00730"
                     /pseudo
     CDS             4228..4925
                     /locus_tag="PLT69_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            5084..6130
                     /gene="mdh"
                     /locus_tag="PLT69_00735"
     CDS             5084..6130
                     /gene="mdh"
                     /locus_tag="PLT69_00735"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOJ13006.1"
                     /translation="MRKKVSFVGAGNVGATAALRLAELDLADIVLVGGVHEGMSAGKA
                     LDMAEAAPLEKHHSRISGYTNDFDEVAGSDIVVISAGVARRPGMSRDDLLTTNRDVVR
                     GLVERIVPIAGDAVFIIVTNPLDAMCHVVLETSGFPRTRVLGMAGVLDSARFATFIAQ
                     ELNVSVENVSAFVLGGHGDTMVPLPRYTCVAGIPVTQLIPSQRLEEIIERTRNGGSEI
                     VNLLKVGSAYYAPACAVKEMCEAILKDKKKILPCSAYLQGEYGVDGLFVGVPVKLGSK
                     GMEEIIEVDLLPQEREAFLRSAQAVRSLVEAMRRLNGKDSLKSQATGQDNRQARQAKR
                     RGSTPRPGVPLTRE"
     gene            6404..8278
                     /locus_tag="PLT69_00740"
     CDS             6404..8278
                     /locus_tag="PLT69_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ13007.1"
                     /translation="MNLERESPPGSSRASGAVLWYVVFNFAVSLCLCYAYILFTPDMD
                     TPLSRVFVHGALVSNTAMLYLVLSLVFGALSLAPGVGVGLLWFMTGFFTVLHMLTVLD
                     IIVFRIFRYHINSMVITLVFTEGARDSLHIGTLTVLTYAAASACVLVLEIWLARLSRR
                     IPSAKPSARRALVFILAFALLFVTAEKATYAFADLFGVKEVTRYNRVFPLYQRLTVKR
                     LARKYLGVKTDPEFKLSASGRTLKYPAQPLVRDPSRGGKLYNIVWIVIDAWRFDMLNA
                     EVTPNILKFSESSLVFANHRSGGNATRFGIFTLFYGLYGTYWHPFLAEGQPPVFLDEL
                     MKLGYDFRIISSSSLSNPEFRRTVFVKLGPYITDHLDGVKADERDPELARTFVDWLNK
                     RDKSRPFFSFLFFDAPHGPYSYPDTFEKFRPSNKNPNYVTAGRKDAHALFNSYKNAVF
                     FDDHLTGMILDALERRGLMEDTIVLITGDHGEEFYETGYWGHTSTFSVYQTGVSFVLY
                     IPGMPHGVIRYPTSHLDVVPTFMRILGYRTPPAAYSHGEDLLGKRPRKYAVVSGWDEA
                     ALADEVYTIVMPTQSHVAAGPEVRLTRGWRLADDERDVLRRRQADILTVMMEMGAFLK
                     "
     gene            complement(8608..9003)
                     /locus_tag="PLT69_00745"
     CDS             complement(8608..9003)
                     /locus_tag="PLT69_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOJ13008.1"
                     /translation="MGKYGDLFQSDDWKKEKHVPVIECPDVVKAEEPFSVKVSLGKEI
                     AHPNTTEHHIRWIQLTFKPEGDKYAYHVGTFEFTAHGECVEGPNKGPVYTGHTATAEL
                     KVSKPGTLVAASYCNIHGLWESSKDIKIG"
     gene            9715..>10870
                     /locus_tag="PLT69_00750"
     CDS             9715..>10870
                     /locus_tag="PLT69_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13009.1"
                     /translation="MVRRAQQGKGRGGQSTARPGNDFLSTLAFVTDLLPDPTFAIDLG
                     GTVILWNRAMEGLTGVSARDMIGRSGYAYAVPFYGEPRPMLVDLLLKQDTVYEARYDV
                     VRRENDTITAEGPVTIAGVRHYLWGKATKLFDPQGKAIGVIELLRDTTDRKAAEEALR
                     QSEARYKDIFLNVSDLLYLHDLNGTFIETNLATKKETGYTEDDLAQMNIRDILPERHR
                     SRFAHYLQSVLEKGADEGVFTIVTKDGRERAFEYRNSLVHDEHGVPVGVRGSARDITK
                     RLELQKQLRRERDFITTIIDTSPAFFDAIDMTGRVIMMNKVMLEALGYAPGEVLGLDY
                     AETFLSPEEREDFASVVRSLVSTKRPVIHEKWMLKKNGERVLVEWHSRTVL"
BASE COUNT         2128 a         3271 c         3564 g         1907 t
ORIGIN      
        1 ccacctcgtc acgatcgggt gcgggtgcca caaccggcgc ctcattgagg aacagtacgg
       61 tgacaccata gagtttctcg actcgtacag gcagctcccc aaggcgctcg agcatctctt
      121 caggagggtg ttcgtctacg gcgcgaagcc gggcaagcct gcccgggcag ctctagacgc
      181 cgcccgggaa acgcttccgg acaagggttg agggaggaca cgtatggaac tcagtcggca
      241 ggtcagggag tacctcgagc aggagggcaa gagcgtcttc atcgcgagct cggacgccca
      301 aggcagggtg aacgtcgcca tgttcggctc ggtgaggctg gcgccggacg ggatggtcga
      361 gctcatgctc ggggacaaca gaacctggtc gaatctcaag gaaaacggct cggccgcgtg
      421 catggtgacc atgcacggta ccagcggcct gtccacgaag gggtgcaggc tttacctgag
      481 gttggccgag gccaaggacg agggggcaga cgtggaggcg ttcagggaac gccttgcagc
      541 ccgcatcggg aaggcggccg acatgatgag gcacctcgtc aggttcgagg tcgtcgaggc
      601 gaggcccatc atcgatctcg gccagggagt ctgatccttg aaagggcttg agggataccg
      661 ggacatgagg aatagtcggg tcaccactca tgaagcaaag gagggacatt ccatgaacga
      721 ccagaccgtc ctcttgaccg aggtgcgctc gaacatcggc accatcaccc tcaacaggcc
      781 acacagcaag aatgcgttgt ccatcgatct cctcgtggag atccacagga cgctcactgc
      841 ctggtcccga tcggacgaca tcaggtgcgt gatcgtaaca ggcgggacgt ccagggcgtt
      901 ctcgtcggga tacgacatct cctcgatccc aaccatgatg acgcccgaga tagaggaggt
      961 catgcacagg ggaaacccgc tggagttcgc cctggatgcg gtcttctcgt acccgtatcc
     1021 caccatcgcc atgatgaacg gctacgcctt cggcgccggc ctcaacctcg cactgtgctg
     1081 cgacatgcgc atcgccgcgt cggacgtgaa ggtggggatg ccccctgcga ggcttgggct
     1141 cgtctatcac ccggacggcc tgagacgttt catcgaggtc gtgggaatgg caaggacgag
     1201 ggaaatcttc ttcacgggga atacgtacga gggggaggag gccctcgcca tagggctggt
     1261 cgaccggctc gtgccgagag aagagctcga agacacggta tatgccgtcg ccgagaccat
     1321 agcgggcaac gcccccctcg ctctcaaggg caccaagcgc atcctctcga tgatatccga
     1381 gcgcacgagg ctcggcgagg aagagatgaa cgaggcgagg aggctcatgc gcgaggcgtt
     1441 cgaaagcgac gacctcaagg aaggccaggt cgcgttcttc gagaagaggc ccccgcgatt
     1501 ctcggggaag tgacgaagga acggggttct atcgtgagtg attgacggtg atgggatgcg
     1561 gctgggtgcg agaaaggggg gaaaggaggc gtgttcgtcc atggccatcg aatggaacat
     1621 aggctttatc ccgtacaagc gtgcgcagct gacacccgac aggacggccc tcgtctacga
     1681 ggaccagccc gtcacctacc gcgagctcaa ccagagggtc aacaaggcgg cccacatgct
     1741 cgagaagaag gggatcaaga agggtgaccg cgtggccctg ctcctgctca actgcgtgga
     1801 gttcctcgag gtctatttcg cgctcgcgaa gctcggagcc atcctcgtcc cgctcaactg
     1861 gaggctcgcg gggcctgagc tcgagtacca gctcaacaac tgcgacgtga ggatgctcgt
     1921 attccacgac gtgttcctgg gcagcgtgga catcatcagg gagaggataa aggtggagca
     1981 ggacaagttc ctgttcctgg agagcgggtc aaagggcctt ccaggtctcg aaccgcccaa
     2041 gtgccccttc tgggcgcagg actacctctc gctcgtcgag cccgagagca ccgacgagcc
     2101 cgagccgtgc gagccggtga ccctcgacga ccccctggcc atcctctaca cctcgggcgt
     2161 gaccgggtcc cccaaggggg cggtggtctc ccacctccag accttcttta agaatttcca
     2221 ggtggggctc tacatcgacg cgtcccagga cgacatcgtg atcgcccaga tgccgctgtt
     2281 ccactcggga gggctcttca tcgtcgcgac gccggcgttg tgtgcgggca tgacgctcgt
     2341 catgaggagg ggcttcgacc cgaacgagtt cgccgaggac atccagcggt acaggggcac
     2401 catcgtgttc gccctcacca cgatgtggcg catgatcctc gagaccggaa agctcgactc
     2461 gatcgacgtg aattccgtca ggcgcgtggt gggcggcggg gagaggaccc cgccctcgct
     2521 gttcgacgag ctcgccaagc gcaacctctt catgcagcag gggttcgggc agacggagaa
     2581 ctcggccatg atgctcctcc cccgtgagga catcaagcgc aagatggggt ccatcgggaa
     2641 gcccggtttc tttaccgagg tctggataga cgacgggaag ggcaggaggc tgcctcccgg
     2701 ggagatcggc gagatctgcg cgaaaggccc gacggtcatg agcgggtact ggaacatgcc
     2761 cgaagagacc gcgaaggcag taaccgacgg cggagtgctc cacacgggag acctggggta
     2821 catggacgag gaagggtact tctacatcgt cgaccgcgtc aaggacatgt acaggagcgg
     2881 cggggagaac gtctaccccg ccgaggtcga gaagatcctc tccacccacc cgaagatctc
     2941 gatggtggcc atcatcgggg tgccggacga tcgctgggga gagaccggca tggcgtttgt
     3001 cgtgccgaag aacggcgaga agatcacgga gaacgaggtg ctcgactacc tgaaggacaa
     3061 ggtggcgagg tacaagtatc cctcgaaggt catgttcatg gacgagttgc ccctgacggc
     3121 caccatgaag gtgaagaagg cggaactcaa ggcgaggtac ggcagggtat agggtatgac
     3181 gacgcggggt ttcagcaaca gggtgaacga gctcttgggc tcgcgttatc ccatcgtggc
     3241 cggtcccatg cggctcatct cccttggggg catggccgcg agcgtgtccg aagcaggcgg
     3301 cttcggcgtc atcgcggcct ccggcctgaa cgccaaggag ctcgagcgcg aggtgagcct
     3361 cgcgcgctct ctcacggaca ggccgttcgg cgtgaacatc cccctgtacc gggaaaacgc
     3421 ccgggaggtc atcgatgccg ccctggccat gggcataggc gtcatctaca cctcggcggg
     3481 tgacccgcag aggatggtgg agaggatcaa gtcgcgcggg gccaaggtga tacacaaggt
     3541 ctccagcctg aacatggcgc gtaaggctgc cggggcgggc gtcgacgcgg tggtggccat
     3601 ggggtgcgag gcagggggac acatcgggaa agacctcgtg acgatgttct gcctcgtgag
     3661 atccatcacg cgcacgctcg acatcccggt catcgcctcg gggggcatag gggacgggag
     3721 ggggctcctc gctgcgctca tcctcggggc cgagggcgtc gagatcggca cgaggctggt
     3781 ggccacgagg gaatgccccg taccggcctt cttcaaagag gcattgtgca gcgcggagtg
     3841 cacctccacg ctcgtgctcg gcagggagac gatgcccata cgcgttctgg ccaacgccat
     3901 ggccttgcgc gccgccggca tggcgtcagg cagcctggat gtggagatgg ctgcgagggg
     3961 agacgatctg tacgcacccc gtgcgcgcga gaaagactcc gccatcatgc cctgcgggca
     4021 ggttgccggg ctcgtggacg gggttctcga ggtccgcgag gtgatcgatt cgatggtcag
     4081 ccaggccagg gccatggctc gaggtatcgg cgagagcgaa gggtggtggc ttgcatgaac
     4141 ttcgagcaca tcatcctgga cgtggacgac atggtggcga cgatcaccct gaacaggcct
     4201 gaacgcaaga acgccttcgg cggtctcatg cgccaggaac tcgtggatgc cttgacggag
     4261 gtcgcagtcc gcgaggatgt gagggtcgtc gtgatcaccg gtgcggggga tgccttctgc
     4321 accggtggcg acgtggggga gttcgcctcg ggcacgacga aggcgctgtc caaggtcgca
     4381 agcaacgagc gtcatgccat gtgccgggcc gtgctcgcca tcgattccat ggagaagccg
     4441 gtgatcgcat cggtgaacgg gatcgccgcc ggtgggggat gcaacctcgc ccttgcctgt
     4501 gacatgcgga tagcctcgga cagggcgaga ttcagccaag tcttcgccaa gaggggcgtc
     4561 cacccggact ggggggcatc tatttcctgc cccgcctcgt ggggtactcg agggcgtgcg
     4621 agctcgtgtt cacggccagg accgtggacg ctctcgaggc gctgtcgatg gggctcgtca
     4681 acaaggtggt cccgcacggg cgcctcgagg cggaaacgag ggaacttgcg gggaccatcg
     4741 catcaaacgc gccgcttccg atagcgttcg caaagcgagg tctcaggaat ttctgcaggt
     4801 gggacctccc ccaggcgctc gactacgagt cgtacgtgct cgaggtgctc atgaagagcg
     4861 aggatatcgt cgaggggttc tccgccttcc tcgagaagag gcggccccgt ttcaagggga
     4921 gataggctgg ggactgcatc ggtacgggag tcggccgcgt tggactaaac accgaggcat
     4981 tgtttggaag ccggccgaag gcgcgaaaaa ggcgatgccc ggctcaggga cccgggcggc
     5041 catgggagag acgtgttcgg tgcaacctga agggaggcag aagatgcgga aaaaggtatc
     5101 gttcgtgggt gcaggcaacg tcggtgcaac agcggcgctg aggctggccg agctggatct
     5161 cgcggacatc gtcctcgtgg gcggggtgca cgagggcatg tcggccggga aggccctgga
     5221 catggccgag gcggcccccc tcgagaagca tcacagcagg atatccgggt acaccaacga
     5281 cttcgacgag gtcgcgggct ccgacatcgt cgtcatctct gcgggagtgg cgaggagacc
     5341 gggcatgagc agggacgatc tcctcacgac caaccgcgac gtggtaaggg gcctcgtcga
     5401 gcggatcgtc cccatagccg gggatgccgt gttcatcatc gtgaccaacc ccctcgacgc
     5461 catgtgccat gtggtgcttg agacgagcgg gttcccgcgc accagggtgc tgggcatggc
     5521 gggcgtcctc gactccgcca ggttcgcgac gttcatcgcg caggaactga acgtctccgt
     5581 cgagaacgtc agcgccttcg tgctgggcgg gcacggcgac accatggtgc cgctgcccag
     5641 gtacacgtgc gtggccggta tcccggtcac ccagctcata ccctcgcaga ggctcgagga
     5701 gatcatcgag aggacacgca acggcggctc ggagatcgtg aacctcctca aggtcggcag
     5761 cgcctactat gcgcctgcct gcgcggtcaa ggagatgtgc gaggccatcc tcaaggacaa
     5821 gaagaagatc ctgccctgct cagcctacct ccaaggggaa tacggggtgg acggcctctt
     5881 cgtgggtgtc ccggtgaagc tcggctcgaa gggcatggag gagatcatcg aggtcgatct
     5941 gctgccgcag gagcgcgagg cctttctcag atccgcacag gcggtccgct ccctcgtcga
     6001 ggcgatgcgg aggctcaacg gaaaggacag cctgaagagt caggccacgg gtcaggacaa
     6061 caggcaggca cggcaagcga agcggagggg ttcgaccccc cgcccggggg ttcccctgac
     6121 ccgggaatga aggggccgca ccccccgcga gggtgcatcc gcaggtctcg acccccagac
     6181 gataggcccc tcttgggtgc gggggttgag acggcgggct cgaagggcga gagacggacg
     6241 acataaccca tgccgcttgt tttttgaaag gcccgggccc atccggtgcg tttcctttca
     6301 cggaaaccgt ccgatgaccc caccgtaggc cgggtgagcc cgggcccgtt ttttccccaa
     6361 tactttaccg agggcggcct ctgtgctaat gaggccaaag gccatgaatc tcgagcggga
     6421 atccccacca ggaagcagtc gggcgagcgg cgccgtcctc tggtacgtgg tgttcaactt
     6481 cgcggtctcc ctctgcctct gctatgcata catcttgttc actcccgaca tggacacccc
     6541 cctcagccgg gtgttcgtgc acggggcgct cgtctccaac acggcgatgc tgtacctcgt
     6601 tctctccctg gtgttcggtg ccctgtcgct ggcgcctggc gtcggggtgg ggctattgtg
     6661 gttcatgacg gggttcttca ccgtgttgca catgctcacc gtgctcgaca tcatcgtgtt
     6721 caggatcttc aggtaccaca tcaactcgat ggtcatcacc ctggtgttca cggagggcgc
     6781 cagggactct ctccacatcg gcaccctcac cgtgctcacg tacgcggccg cctcggcctg
     6841 tgtgctcgtc ctcgagatct ggctggcccg cctgtcgagg aggatcccct cggccaagcc
     6901 ctccgcacgg agggccctcg tcttcatcct tgcgtttgcc ctgctcttcg tgaccgccga
     6961 gaaggccacg tacgccttcg ccgatctctt cggcgtcaag gaggtgacca ggtacaacag
     7021 ggtcttcccc ctctaccagc gcctgacggt aaagcgtctc gcccgcaagt acctgggcgt
     7081 caagacagac cccgagttca agctgagtgc gtccgggcga accctgaagt acccggccca
     7141 acccttggtc cgggatccgt ccaggggggg aaaactctac aacatcgtct ggatcgtgat
     7201 cgacgcgtgg cgtttcgaca tgctcaacgc ggaggtcaca cccaacatcc tgaagttctc
     7261 ggagagttcc ctcgtgttcg caaaccacag gagcgggggc aacgccacgc gtttcgggat
     7321 cttcaccctc ttctacgggc tgtacggcac gtactggcac ccgtttttgg ccgaggggca
     7381 gccgcccgtg ttcctcgacg agctcatgaa gctcggttac gatttcagga tcatctccag
     7441 ctcgtccctc tcgaacccgg agttcagaag gacggtgttc gtgaagctcg gcccgtacat
     7501 caccgaccat ctggacggcg tcaaggccga cgagagggac cccgagctgg cgcggacctt
     7561 cgtggattgg ctcaacaaga gggacaagtc acgcccgttc ttctctttcc tcttcttcga
     7621 cgccccccac ggaccttact cctaccctga caccttcgag aagttccgcc cgtcgaacaa
     7681 gaaccccaac tacgtcacgg ccggcaggaa ggacgcccac gccctcttca acagctacaa
     7741 gaacgcggtc ttcttcgacg atcacctgac cggcatgatc ctggacgcgc tcgagaggcg
     7801 cggcctcatg gaagacacca tcgtcctcat cacgggggat cacggcgagg agttctacga
     7861 aaccggctac tgggggcaca cgagcacgtt ctcggtctac cagaccgggg tgtcgttcgt
     7921 cctgtacatc ccgggcatgc cccatggcgt gatacggtac cccacgagcc atctcgacgt
     7981 ggtgcccacc ttcatgcgca tcctcgggta ccggacgccc cctgcagcct actcccacgg
     8041 cgaggatctc ctcgggaaac gtccgaggaa atacgcggtc gtctcggggt gggacgaggc
     8101 ggccctcgca gacgaggtgt acaccatcgt catgccgacg cagagccacg tggccgcggg
     8161 ccccgaggtc cgcctcacgc ggggctggcg tctcgcggac gacgagagag acgtgctcag
     8221 gcgaaggcag gccgacatcc tcaccgtgat gatggagatg ggcgccttcc tcaagtgatg
     8281 cgggcacccc cggtccaggg gtgaaggcca tcacgcacgc tgctgatctc agacaggcat
     8341 cctcgaaaac acagctggaa gaccggcatc acccggtgca ggcccggatc gtgtggtggt
     8401 gcgggctgaa gggacgagta cctaccggtc atggaagagg gtggatgccg agcctgcctc
     8461 ccggaaaggc tttcccggag gctttgcggg gagtgacccg cggccgccgg gtgcgggacc
     8521 acctcaaggt ggccggtgag cgcgcagggc ggccactagg gcctcgggac ggcccggctc
     8581 atgaatgccc gcgctgcagg cgggatctca cccgatcttg atgtccttcg aactctccca
     8641 gagcccgtgg atgttgcagt acgaggccgc gaccagggtg ccgggcttgc tcaccttgag
     8701 ttcggcggtc gcggtgtggc cggtgtacac ggggcccttg ttgggtccct cgacgcactc
     8761 gccgtgggcg gtgaactcga aggtgcccac gtggtatgcg tacttgtcgc cctcgggctt
     8821 gaaggtgagc tggatccacc tgatgtggtg ctcggtggtg ttggggtggg cgatctcctt
     8881 gcccagggac accttcacgg aaaacggctc ttccgctttt accacgtccg ggcactcgat
     8941 gaccggcacg tgcttttcct ttttccagtc gtcactctga aacaggtcgc cgtatttccc
     9001 catgtctcat cctccttttc gttctcacgg tctgaaaggg agcgcccgcc tcgggcggga
     9061 ccccatccgg ccgacatgac agggttcgtc gtcggacagg gcgcccgagc agcctccgcc
     9121 ggcgtgatgc cgcatgccaa aggtgagtgg tcattctagc acgaaaccgc cttcgtgcaa
     9181 aaggccgggc aagagagacg ttgtccgggt cttcaggggg ccaggggccg gccgagcagg
     9241 aaccttcagg ggggggattt cagccgtcac cgatcgtgcc ctatccccag gctcgttctt
     9301 cagtatgcaa tttgcgacgg tcctgcatcg agcccgtcat ccctgtctga agacgcctgt
     9361 atcgatgaag gtatgtccgg gttcaagcct cggatgaggg tgataacggc gaacaatgag
     9421 aagatagcaa tgggctcatg gtccacagta cacatcgaga gtattgacgg cgaggaacgc
     9481 ccgggtccca gggtgccgtg ttaatcgtgc aacgtgcgtt gcgtgtcttg tggtgcagtt
     9541 cgatatcgga gcttgacggg tcgaaggatg agtataatat gtaggtcaac gtcgctccag
     9601 atccggcggg tagggcagcg tggttctccc ctatgcggaa cgttcgatcc cgggagaaaa
     9661 caatcatttt ccagcacgta acggcgatga cgtcgaccaa gtgtcacgct ccatatggta
     9721 agaagagcgc aacagggaaa aggccgaggc ggacagtcca cagctcgtcc gggcaatgat
     9781 ttcctctcca ccctcgcctt cgtcaccgat ctcctccctg atcccacgtt cgcgatcgat
     9841 ttgggcggca ccgtgatcct ctggaaccgg gcaatggagg gcctcaccgg ggtcagtgcg
     9901 cgggacatga tcggcaggtc gggctacgcc tacgcagtac ctttctacgg cgagccgagg
     9961 cctatgctcg tggatctcct tttgaaacag gacaccgtgt acgaggcacg atacgacgtg
    10021 gtcaggcgcg agaacgacac gatcaccgcg gaaggtcccg tgaccatagc gggagtcagg
    10081 cattacctct ggggcaaggc cacgaaactc tttgaccccc aggggaaggc catcggcgtc
    10141 atcgagctgc tcagggacac gacagacagg aaagcggccg aggaagccct caggcagagc
    10201 gaggccaggt acaaagacat ctttctcaac gtatcagacc tgttgtacct ccacgatctg
    10261 aacggaacat tcatcgagac caacctcgcc accaagaagg agaccgggta cacggaagat
    10321 gaccttgcgc aaatgaatat ccgggacatc cttcctgaaa gacaccgctc gcgattcgca
    10381 cactacctgc agagcgtcct ggaaaagggc gcggacgagg gtgtcttcac catcgtaacc
    10441 aaggacggaa gagaacgcgc cttcgagtac cggaactcgc tcgttcatga cgagcatggc
    10501 gttccggtcg gtgtacgcgg ttccgcccgg gacatcacga aaagactcga gctacaaaag
    10561 cagctccgcc gtgaacgcga tttcatcacc accatcatcg acacctctcc ggcgttcttc
    10621 gatgccatcg acatgacggg tagggtcatc atgatgaaca aggtcatgct cgaagccctg
    10681 ggatatgcgc cgggagaggt tcttggtttg gactacgcag aaacgttcct ttcgccggag
    10741 gagcgggagg acttcgcatc ggttgtccgc agcctcgtct cgacaaagag gcccgtgatc
    10801 cacgaaaagt ggatgctcaa gaagaacggt gagagagtcc tcgtcgagtg gcacagcagg
    10861 acggtactca
//
LOCUS       DAOSCR010000011         9862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4500_length_9862_cov_3.882737, whole genome shotgun sequence.
ACCESSION   DAOSCR010000011 DAOSCR010000000
VERSION     DAOSCR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_9862_cov_3.882737"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLT69_00755"
     CDS             <1..702
                     /locus_tag="PLT69_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOJ13010.1"
                     /translation="ELDELLHGGLERGTVTLLTGPSGVGKSTLGMAFMKEAAGRGERS
                     VMYTFEEEVELLLARCEAVNIPAYIMVERGTLAVEKIEPLRYSPEEFACAVRREVEGK
                     GSRIVMLDSVSGYRLSLRGEDMTGHLHALCTYLKNMGVTVLLVNEVEHITGEFRATEV
                     GISYLADNIVFLRYLEMQGELHKAIGVLKKRLSDFERTLREFQITRYGLKVGKPLVGL
                     RGILTGTPEWTRPPG"
     gene            699..1079
                     /locus_tag="PLT69_00760"
     CDS             699..1079
                     /locus_tag="PLT69_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13011.1"
                     /translation="MRDGPPRPSPAILVAGGNRRNLELLGRSLEADGWRVLTAACMEE
                     LDRALSVPEEIGLALVDIAGFGRDIWERCESLRARGVMLLVISPRRSQAIQQESLAHG
                     ARGVLVKPLSVREVMTLVRGLLRE"
     gene            1737..2897
                     /locus_tag="PLT69_00765"
     CDS             1737..2897
                     /locus_tag="PLT69_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ13012.1"
                     /translation="MAKILLMVPHGPNRDLIAACLAHNHEVIGVEDDDLGKSFDLGIV
                     DARVLSMKRGLIVERRHSEDFFLPFMAVVSRKDVTLISSHLWEVVDDLVTAPIMKIEL
                     CARVEILLRARRLSIEVREHEIAGRIMSEAKAELERMVEERTAELRASNDRLMELDRL
                     KNMFIAAMSHELRTPLNAIIGFTGVMLQGISGEITKEQQKQLGIVMDSARHLKSLIDG
                     LIDVGRIESGWVDMHPEDVDVEGLVREVAGMMEAMASSKGVRVEVRTRGPLGTLTDRK
                     LLKQVLVNIVGNAVKFTERGVVSIDAARERGVLVIEVRDTGRGIPAERMEDLFAPFCR
                     IEPDQDKPREGAGIGLYLSKKIVNHLGGDICAESTYGQGSTFKVMLPAGRAG"
     gene            3072..3815
                     /locus_tag="PLT69_00770"
     CDS             3072..3815
                     /locus_tag="PLT69_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13013.1"
                     /translation="MRILIVDDQEYSRYLLETILKASGHEVTAAVNGLDALEKMERAG
                     FDLVISDILMPVMDGYTLCRRCRADERFRDILFVFYTGTYTDERDEALAYSLGADLFL
                     RKPLSPNELVAKLEGLLSAGRSGPAPTSPGEVDGGVLELYNERLVRKLEGKMQELEEE
                     RGRRNAILLEVQRKNRSLRMIKECMSALMHSREEQDLFSSIARVVVDSGGFSVCWIGM
                     VGPDGLVHPEASWCRLPGVEFRVPQACLG"
     gene            3834..5639
                     /locus_tag="PLT69_00775"
     CDS             3834..5639
                     /locus_tag="PLT69_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13014.1"
                     /translation="MAECMRSGKIAVRTPPWDGLCRDVSLNSVKFGYVGEMAVPLVVQ
                     GRPIGVMLLYVPEQAALDEEDVSLLSGLAEDMAVHVVSLRNERERLAAETLLARSEER
                     FRIMAETNPDTIFLLAREGLVEYLSPAAGRLFGRPVTELTGMRFTDFFDGDDALDAVR
                     AFDEVCSGATVRNRELVISLPGGGKSFIEISAAPLVKEGVVEGVLGVARDIGRRKEIE
                     SQLLASQKMEVLGSMAAGIAHDFNNILSAIVGYAELMLEKAGSDAAGSKDLAAILDAS
                     MKARDLVRGLLNLSRLTPEKPTALRPAEVIRETMRLMRSCVHANIEIALNARSTTRIL
                     SDPARLHQMVLNLVVNAAAAIGSRPGRITIDVEDTQAPAGVQGTAAAPRTYVKVSVSD
                     TGCGIAPEILPDIFKPFFSTRHDRGGTGLGLFMVKTLVEEHGGFVEVNSVVGSGTRFD
                     LLFPAFEGEGEGVQAESEEESPRGSGRILFVDDEQSIAVIAHEMLSGLGYQVTSVSSG
                     IEALDLFGKDPTGYDLVVTDVAMPVMTGVDLAREVLKIRPGMPVILCSGTHDGLLETE
                     AEALGVRAFLLKPFKRLDLARVVMSAMGAPRLTAG"
     gene            complement(5657..6475)
                     /locus_tag="PLT69_00780"
     CDS             complement(5657..6475)
                     /locus_tag="PLT69_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOJ13015.1"
                     /translation="MDRGYGVRLERQGPVAVVTFDRPERKNAFDEHMWGCLEAAVADI
                     RQRPPRAVVVTGAGGRAFSAGFDVNPDNPMVERLARAVERHDRGPAWELITRIRGAVD
                     SLVSIEVPVIAAINGLAYGGGAEIACRCDLRVMDPSGVICFSETRLGLMPDHGGVVGL
                     ARLVGASRAADLILTAREVGAEEALSMGLVNRVSAPGKALDEAMDLACAIASNGPRAV
                     RSALSVIRRVADLDALSALELETETAVDLIASGECIHGITAFLSKQKAVFPDPA"
     gene            complement(6482..7042)
                     /locus_tag="PLT69_00785"
     CDS             complement(6482..7042)
                     /locus_tag="PLT69_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ13016.1"
                     /translation="MELQDAIERRRSVRKFTDEPITDEEIRRLLEAARHAQSWANTQV
                     WEFVVVRDRQSIEKIVSTYAQGNPATKCSLAASALVVACAKTGVSGCYGGKDVTKFSN
                     WFMFDLGVAVQNFCLKACELGIGTVVVGLLDHDACKEVVGLPEGYEVVAVLPLGRPAT
                     EPKAGPRRKDLGEFVHLDRFGTPFPG"
     gene            7268..8449
                     /gene="larC"
                     /locus_tag="PLT69_00790"
     CDS             7268..8449
                     /gene="larC"
                     /locus_tag="PLT69_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOJ13017.1"
                     /translation="MKVLVVDPAGGVAGDMILAGLIHLGCPPGLVEESLDALGLGRCG
                     LKVTRREEGGVGCLHVEFEANRDDEQRPYRRIRDVILPRLEPGPRDLAARIFEVLARA
                     EAAVHGVEPEDVHFHELGALDSIFDIVGIAVALDRLGVSRLYTRPVPLGSGWVDSSHG
                     RLPVPAPATMRLLEGFPVRFEGPPSEIATPTGAAVIAALASPLRRPPVLRVSGTGYGC
                     GSKRFENWPNVCRLVLCEDEGSCGCERVFVVEADIDDMTPEDASSALARIMEAGALDA
                     GFTHRTMKRSRPGYTIQAICPEGVLARVVESFVKHTSTLGVRYHAVERLALPRRVYGV
                     DTPFGEISVKEAVLPDGTVRVKPEHRDLERISSRTGKSVLELRAEVELAIAGRKGDEP
                     T"
     gene            8807..9607
                     /gene="larB"
                     /locus_tag="PLT69_00795"
     CDS             8807..9607
                     /gene="larB"
                     /locus_tag="PLT69_00795"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOJ13018.1"
                     /translation="MDDRGERRTLSERVQNLLDEYKQGRVDAAEAQAGLMRLVYEEGP
                     GLLLDVHRRRRTAFPEVVYAPGKTTRQVIDAAAGLLGENPFVFVWGADGSQEAELRKR
                     FAGMEVRRENRLLLVGRNEAGTGTGCAGIITAGTSDIPYAKECSLMLETLGYGVITAL
                     DMGAAGMHRPLIGLEKARDADVLVVFAGMDGVLPTLVASLTDRPVVAVPTPVGYGYGG
                     AGHAALGTMLQCCVPGVVVVNIGNSVGAAAAAVRILNAVRRAAGGMGG"
     gene            9591..>9862
                     /locus_tag="PLT69_00800"
     CDS             9591..>9862
                     /locus_tag="PLT69_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOJ13019.1"
                     /translation="MEWVDRLAEAIRDKGRLIVLFSGGLDSTVLASVAKRELGGQALA
                     LTFASPVMPASELDAACKTAGSIGIAHVLLPMDELEEAAFAANPPE"
BASE COUNT         1710 a         3061 c         3515 g         1576 t
ORIGIN      
        1 gagctggatg aactccttca cggcggtttg gagcgcggta cggtgaccct cctcaccggc
       61 cccagcggcg tgggcaagag cacgctgggc atggcgttca tgaaggaggc tgcaggcagg
      121 ggcgagcgat cggtgatgta taccttcgag gaagaggtgg aactgctgct tgcccgctgc
      181 gaggcggtca acatccctgc ctacatcatg gtggagcgcg gcaccctcgc cgtggagaag
      241 atagagccgc tgcgctatag ccctgaggag ttcgcatgcg cggttcgcag ggaggtggag
      301 ggaaagggtt cccggatcgt gatgctcgac agcgtctccg gataccgtct ctccctgcgc
      361 ggcgaggaca tgaccggcca cctgcacgcc ctgtgcacgt acctgaagaa catgggcgtg
      421 acggtgctgc tggtcaacga ggtggaacac atcaccggcg agttccgcgc aaccgaggtg
      481 ggcatcagct acctggcgga caacatcgtc tttttacgct acctggagat gcagggcgag
      541 ctgcacaagg ccataggcgt cctgaaaaag cggctgagcg acttcgaaag gacgctgcgc
      601 gagttccaga tcacccgata cggcctcaag gtgggcaagc cgctcgtggg gctgcgcggc
      661 atcctcacgg gaacgcccga gtggacgagg cctcctggat gagggacggg cccccaagac
      721 caagccccgc gatcctggtg gcgggcggca accgccgcaa cctggaattg ctgggccgga
      781 gcctggaagc ggatggctgg cgggtcctca ccgccgcctg catggaggaa ctggaccggg
      841 cgctttccgt gccggaggag atcggcctgg ccttggtcga catagccgga ttcgggcgcg
      901 acatctggga gcgctgcgag tccttgcggg cccggggcgt catgctgctg gtgatctcgc
      961 ccaggcgaag ccaggcaatc cagcaggaaa gcctcgccca cggcgcacgc ggcgtcctgg
     1021 tgaagcccct ttcggtgagg gaggtgatga cgctcgttcg aggcctgctc cgggagtgag
     1081 tgcgaggggg tgctcacctc gggtaagggg gccccctgcg ccgggtcgga ccgagccgta
     1141 aggagaggcg tcagcacggc ccagtgatca tgtctctcga ggagacagtc ctccggaaac
     1201 cccctggtgg tcggtgccgg gcccaacccc aaggataggg cccggcccat cgcgacggaa
     1261 tacgctccca tctgccggat atctttctta gaaaacaggg atgaggccac cccctcgagt
     1321 gtttactgaa cggtgctcag gtggacatac gggcaggggt cattttcgac gagttgaaca
     1381 agggggacat ttctaacgag tcttgacaca aaaggagtga tggcccttga caatccagga
     1441 tgggtggtta ttttgttgat tttgaattgg ctaaaaccct cggcagggtt aaggatcaag
     1501 ggctgctttt cggggaagcg ggaggcaagg cgggatcccc ggaggcgtgg cgaagcctct
     1561 cccaggtaca agtcccggta tgatgggata gccgggctcc ggcagggccg tgagcagggg
     1621 ccgacaaacc gtgtgagggc gtggccgccg cggcgctctc ggctgccgtg gatatgcacg
     1681 gccgcccgga cggcatgggt gggaggaaga acgcgggcgg cggggtgtgt gaggacatgg
     1741 cgaagattct cctcatggta ccgcacggtc cgaacaggga cctcatcgcg gcttgcctgg
     1801 cgcacaacca cgaggtgatc ggcgtcgagg acgatgatct cgggaaaagc ttcgacctcg
     1861 ggatcgtcga cgcaagggtc ctcagcatga agagggggct catcgtggag cgcaggcata
     1921 gcgaagactt cttcctcccc ttcatggcgg tggtctccag aaaggacgta accctcatca
     1981 gcagccacct gtgggaggtg gtcgacgact tggtcaccgc gccgatcatg aagatcgagc
     2041 tctgcgcccg cgtggagatc ctcctcaggg cgaggaggct ctcgatcgag gtgcgcgaac
     2101 acgagatcgc ggggcggatc atgagcgagg ccaaggccga gctcgagcgc atggtggagg
     2161 agaggacagc ggagctcagg gcctccaacg accgcctcat ggagctcgac aggctgaaga
     2221 acatgttcat agccgccatg agccacgagt tgaggacacc gctgaacgcg atcatcggct
     2281 tcacgggcgt catgctccag gggatctcgg gggagatcac caaggagcag caaaaacagc
     2341 tgggcatcgt gatggacagc gcccggcacc tgaaatccct catcgatggg ctcatcgacg
     2401 tggggaggat agagtccggc tgggtcgaca tgcacccgga ggacgtggac gtggaagggc
     2461 tcgtgaggga agtggccggg atgatggagg ccatggcgtc gtccaagggt gttcgtgtgg
     2521 aggtccgcac gaggggaccc ctcgggaccc tcaccgacag gaagctcctc aagcaggtcc
     2581 tcgtgaacat cgtggggaac gccgtcaagt tcacagagag gggggtggtg agcatcgatg
     2641 ccgcaagaga gcggggcgtc ctcgtcatag aggtcaggga cacgggtcgg gggatcccgg
     2701 cggaacgcat ggaggacctc ttcgcgccgt tttgcaggat cgagccggac caggacaagc
     2761 cccgcgaggg ggcgggcata gggttgtacc tctcgaagaa gatcgtcaat cacctgggcg
     2821 gggacatatg cgcggagagc acgtacgggc aggggagcac gttcaaggtg atgcttcccg
     2881 cgggtcgtgc cgggtagggc gcgtcgcagg gtgccgcgcc tgcgcggaaa ggcccatggg
     2941 gcctctcgtg taccgggcgc aaacgggagt gtatgcaggg tgtacggaac ggacgatgcc
     3001 gctggaggtc gcgaagggcc ttctcggtac acagaacggg ttggagggaa aggccgcgca
     3061 ggagaagaac gatgaggata ctgatcgtag atgaccagga gtactcgcgc taccttctcg
     3121 agacgatcct gaaggcatcc ggacacgaag tgaccgcggc ggtcaacggg ctggatgccc
     3181 tggaaaagat ggagagggcg ggcttcgacc tggtgatctc cgacatcctc atgccggtca
     3241 tggacgggta caccctgtgc aggaggtgca gggccgacga gaggttccgg gatatcttgt
     3301 tcgtcttcta caccgggacc tacacggacg agagggatga ggccctggcg tacagcctcg
     3361 gggccgacct gttcctccgc aagcccttgt ccccgaacga gctcgttgcg aaactcgagg
     3421 ggttgctctc ggcggggcgg tccggtcctg ccccgaccag ccccggcgag gtggatggag
     3481 gcgtcctcga gctctacaac gaaaggctcg tgagaaagct ggaagggaag atgcaggagc
     3541 tcgaggagga gaggggcagg cgcaacgcca tcctcctgga ggtccaacgg aagaaccgct
     3601 ccttgaggat gatcaaggag tgcatgagcg ccctcatgca ctcccgagag gagcaggacc
     3661 tcttctcgtc catagcccgg gtggtcgtgg actcgggcgg gttctccgtg tgctggatcg
     3721 ggatggtggg cccggacggg ctcgttcacc cggaagcctc ctggtgccgg ttgccgggcg
     3781 ttgagttcag ggtgccacag gcgtgcctgg gctaaggcta cgaggcgtgc ccgatagcgg
     3841 agtgcatgag gagcgggaag atagcggtca ggacgccgcc gtgggacggc ctgtgcaggg
     3901 acgtctctct caactcggtg aagttcggct acgtcggcga gatggccgtc cccctcgttg
     3961 tccagggcag accgatcggc gtcatgctcc tgtatgtgcc ggaacaggcg gccctcgacg
     4021 aggaggacgt gtctttgctc tcggggctcg ccgaggacat ggccgtgcac gtcgtgtccc
     4081 tgcgcaacga gcgcgagcgg ctggcagcgg agacactcct cgcgcgcagc gaggagcgct
     4141 tcaggatcat ggccgagacg aacccggaca cgatcttcct tctcgccagg gaagggctcg
     4201 tcgagtacct ctccccggcc gcggggcgct tgttcggtcg gcccgtgacg gagcttacgg
     4261 gcatgaggtt caccgacttc ttcgatgggg acgacgcttt ggatgccgtt cgggcgttcg
     4321 acgaggtctg ctcgggcgcc acggtgagga acagggaact cgtcatcagt ctccccggcg
     4381 gtggcaagtc cttcatcgag atcagcgccg cacccctggt gaaggagggg gtcgtggaag
     4441 gggttctcgg cgtggccagg gacatcggca ggcgcaagga gatcgagagc cagcttctcg
     4501 cctctcagaa gatggaggtc cttggctcga tggccgcagg catagcccac gacttcaaca
     4561 acatcctgtc cgccatcgtg gggtatgccg agctcatgct cgagaaggca ggatccgacg
     4621 cggccgggtc caaggacctg gccgccatcc tggacgcctc catgaaggcg agggacctcg
     4681 tcaggggtct cctcaacctg agcaggctga cgcccgagaa gccgacagcg ctccgccctg
     4741 cagaggtcat cagggaaacc atgaggctca tgcggtcctg tgtgcacgcg aacatcgaga
     4801 tcgcgttgaa cgcccgaagc accacccgga tcctgtcgga ccctgcgagg ctccaccaga
     4861 tggtcctcaa cctcgtggtc aacgcggcgg cggccatcgg ctcacgcccg ggcaggatca
     4921 ccatagacgt cgaagacacc caggcccccg ccggggtaca aggcacagct gccgcacccc
     4981 ggacgtacgt gaaggtctcg gtttcggaca ctgggtgcgg gatagccccg gagatcctcc
     5041 ctgacatctt caagcccttc ttctccacca ggcacgacag gggcggaaca ggcctcggcc
     5101 tgttcatggt gaagaccctc gtggaggagc acggcggttt cgtggaggtc aacagcgtcg
     5161 tgggatcggg cacgaggttc gacctcttgt tcccagcctt cgagggcgag ggcgaggggg
     5221 tgcaggccga aagcgaggag gagagcccca ggggctccgg ccgcatcctc ttcgtggacg
     5281 acgagcagtc catcgccgtc attgcgcacg agatgctctc gggcctcggg taccaggtga
     5341 cgtctgtctc gagcggcatc gaggccctcg atctcttcgg gaaagacccg accggctacg
     5401 acctcgtggt aaccgacgtc gccatgcccg tgatgacggg cgttgacctc gcccgcgagg
     5461 tgctgaagat caggcccggt atgcccgtca tcctctgttc cggcacccac gacggtttgc
     5521 tcgagacgga ggccgaggcc ctgggcgtcc gcgccttcct cctcaagccc ttcaagaggc
     5581 tcgatctcgc ccgggtggtc atgagcgcga tgggtgcacc ccgcctcacc gctggatagt
     5641 ggggcggccc ccggtctcag gccgggtcgg gaaacaccgc cttctgcttg gacaggaagg
     5701 cggtgatgcc gtggatgcac tcgccggagg cgatgaggtc aaccgcggtc tcggtctcga
     5761 gctcgagcgc ggagagtgcg tccaaatccg ccacccgccg tatgacggac agggcgctcc
     5821 gtactgcgcg cgggccattg gacgcgatgg cgcaagcgag gtccatggcc tcgtccaggg
     5881 ccttgccggg cgcgctcacg cggttgacca gccccatgga cagcgcctcc tcggcgccga
     5941 cttccctcgc ggtgaggatc aggtcagcgg cccgggacgc cccgacgagc cttgcgagcc
     6001 cgaccacgcc gccgtggtca ggcatgaggc cgagcctcgt ctccgagaag cagatgacgc
     6061 ctgaggggtc catgacccgg aggtcgcacc tgcacgcgat ctcggccccg cccccgtagg
     6121 cgaggccgtt gatcgccgcg atgacgggca cctcgatgga cacgagggag tcgaccgcgc
     6181 cgcggatcct cgtgatgagc tcccatgcag ggccgcggtc gtgcctctcg acggcgcgcg
     6241 cgagcctttc caccatggga ttgtcggggt tgacgtcgaa gccggcgctg aaggccctcc
     6301 cgcctgcgcc cgtgacgacc accgcgcgcg gcggcctttg cctgatgtct gcaacggcgg
     6361 cctccaggca cccccacatg tgctcgtcga aggcattctt ccgctccggc ctgtcgaacg
     6421 tgaccacggc cacgggccct tgcctctcga gcctgacccc gtaccccctg tccacggcgc
     6481 cctaccctgg aaacggggtc ccgaagcggt ccaggtgcac gaactcgccg agatccttcc
     6541 tgcgcgggcc cgccttcggc tccgtggccg gcctgcccag gggcagcacg gcgaccactt
     6601 cgtagccctc cggcagcccc acgacctcct tacacgcgtc gtggtcgagg agccccacga
     6661 ccacggtgcc gatcccgagc tcgcaggcct tcaggcagaa gttctggacc gccacgccca
     6721 ggtcgaacat gaaccagttc gagaacttgg tcacgtcctt gccgccgtag caacccgaca
     6781 cccctgtctt cgcgcaggcg accacaaggg ccgaggcggc gagggagcac ttcgtcgccg
     6841 ggttgccctg cgcgtacgtc gagacgatct tctcgatgct ctgcctgtcc ctcaccacca
     6901 cgaactccca gacctgggtg ttcgcccacg actgggcgtg cctggccgcc tcgaggagcc
     6961 gccgtatctc ctcgtcggtt atgggctcgt cggtgaactt tcgtacgctc ctgcgcctct
     7021 cgatggcatc ctggagttcc acgccacacc cccctttcat ggaatgcata gtcttgcctg
     7081 ggtactcccc tccccatcat gcaccctgaa cgaagacggc gcaacaccca acatcaggcc
     7141 gaggtgtcat cggggatggc gcggcctgtc gagactctct tgccgcgggt gacgaaaaag
     7201 ggtagtgatc aggagacgtt tcggatgacg acagaccggt tcacaccatg accacagggg
     7261 gctctccatg aaggtgctcg tcgtggatcc cgcaggcggc gtcgcgggcg acatgatcct
     7321 ggccgggctc atccacctcg gctgccctcc cgggctcgtc gaggagtcct tggacgcgct
     7381 cggcctcggc aggtgcggcc tcaaggtgac gaggcgagag gagggcggcg tcggctgcct
     7441 tcatgtcgag ttcgaagcga accgggacga cgagcagagg ccctaccgcc ggatacgcga
     7501 cgtgatcctg ccgaggctcg agcccggccc ccgggacctg gcggccagga tcttcgaggt
     7561 cctcgcccgg gccgaggccg ccgtgcacgg ggtggaaccc gaagacgtgc acttccacga
     7621 gctcggcgcc ctggattcca tcttcgacat cgtgggcatc gccgtggccc tcgatcggct
     7681 cggcgtgagc aggctctaca cgaggcccgt gccgctcggg tcgggctggg tggactcgtc
     7741 ccacgggcgg ctgccggtgc ccgcccctgc gaccatgagg ctgctggagg gtttcccggt
     7801 taggttcgaa gggcccccgt ctgagatcgc cacccccacg ggcgctgcgg tgatcgcggc
     7861 gctcgcaagc cccctgcggc ggccgcccgt gctcagggta tcgggaaccg gctacgggtg
     7921 cggatccaag aggttcgaga actggcccaa cgtgtgcagg ctggtgctct gcgaggatga
     7981 ggggtcttgc gggtgcgaga gggtcttcgt ggtggaagcg gacatcgacg acatgacccc
     8041 cgaggacgcg tcctcggccc tcgcgcgcat catggaggcc ggcgcgctgg atgcgggctt
     8101 cacgcacagg accatgaagc gctcgagacc gggctacacc atccaggcga tctgcccgga
     8161 gggcgtcctg gcccgggtcg tcgagtcgtt cgtgaagcac acgtcgaccc tcggcgttcg
     8221 ctaccatgcg gtcgagcgcc tcgcgctgcc gaggcgggtc tacggcgtgg acacgccctt
     8281 cggggagatc tccgtcaagg aggcggtgct gccggacggc accgttcgtg tcaagcccga
     8341 gcacagggac ctggagcgga tatcgagccg gacaggcaaa agcgtcttgg agctcagggc
     8401 ggaggtagag ctcgccatcg cgggccggaa aggggacgaa cccacctagg ggcgtgggtg
     8461 agggtcaggg gtcggcccgg gtgtgcggcg agccccgcgg gtccgtgggg accggcggat
     8521 tcacagggtc ggcccgtggg caggaaaggg cccggccgcc ttgcccgacg ctaaccctca
     8581 ggccgcctta aggtgtccag gccgcacccg gtgccaggcg ggttccgccg gtcgcgtggg
     8641 gccccgggca cgaagcgggg tctcccaggg gccgcgcggc ccctcgcggg acgggcgagg
     8701 cctcgtgccc ggaggtggtg ggcacggggg gtaaacgacg ggagaggtca caggggatgg
     8761 gtcgtcggcg ccgggcggac ccgggccgcc tgatgggggg atttcgatgg atgaccgcgg
     8821 cgagaggcga acgctttccg agcgggtgca gaacctgttg gacgagtaca agcaggggcg
     8881 cgtggacgcc gccgaggccc aggccgggct catgaggctc gtctacgagg agggccccgg
     8941 gctcctcctc gatgtgcaca ggagacggcg caccgccttc ccggaggtgg tctacgcgcc
     9001 gggaaagacg acgaggcagg tgatcgacgc cgccgccggg ctgctcgggg agaacccctt
     9061 cgtgttcgtc tggggtgcgg acgggtccca ggaggccgag ctcaggaaac gctttgcggg
     9121 catggaggtg aggagggaga acaggcttct cctcgtgggc aggaatgagg cgggaaccgg
     9181 gaccggttgt gccggcatca tcacggcggg gacctcggac ataccctatg ctaaggagtg
     9241 cagcctcatg ctcgagacgc tcgggtacgg cgtaatcacg gccctcgaca tgggggcggc
     9301 gggcatgcac cgtccgctca tagggctcga gaaggcccgc gacgcggacg tgctcgtcgt
     9361 gttcgcgggc atggacgggg tgctcccgac gctcgtggcc tcgctcacgg acaggcccgt
     9421 cgtggcggtg cccacccccg tggggtacgg ctacggggga gcgggccatg ccgcgctggg
     9481 caccatgctc cagtgctgcg tccccggggt ggtcgtggtg aacatcggca acagcgtggg
     9541 cgctgccgcc gcagcggtac gcatactgaa cgccgtgagg agggccgctg gtggaatggg
     9601 tggataggct tgcggaggcg atcagggaca agggccgtct catcgtgctc ttctccggcg
     9661 ggctcgacag caccgtgctc gcgtccgtgg cgaagaggga gctcggcggg caggccctgg
     9721 cgctcacctt cgcctctccc gtcatgcccg cgagcgaact cgatgcggca tgcaagaccg
     9781 ccggctcgat agggatcgcc cacgtgctcc tccccatgga cgagctcgag gaggctgcgt
     9841 tcgcggcgaa cccgcccgag ag
//
LOCUS       DAOSCR010000012         9798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4542_length_9798_cov_3.139279, whole genome shotgun sequence.
ACCESSION   DAOSCR010000012 DAOSCR010000000
VERSION     DAOSCR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4542_length_9798_cov_3.139279"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLT69_00805"
     CDS             complement(<1..565)
                     /locus_tag="PLT69_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13020.1"
                     /translation="MKIARKSRIVFFLFLVACALQATGSAQQVHIDVEAPGFARLKVA
                     MPPFAGDPATAQSLWKVCARDLAITGLFDVIDPASYVNPAPAPRIALQSLKDYALIGA
                     DYAVAAMVTIQDGAVRVRFHMVEVSTATVVDDTLYTASAGEPHRAVHAFLGSQLESDL
                     LGRRRGISRTTTTPPPRTGPPSLGTRRR"
     gene            complement(562..1269)
                     /locus_tag="PLT69_00810"
     CDS             complement(562..1269)
                     /locus_tag="PLT69_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOJ13021.1"
                     /translation="MGRYLAISVGCHAVILLAFLGFVWSGPSADSQGVYEVEIVAGGP
                     SGEAAADLGGAAAGAPGKRFVYSRTGRAVSIGEVAKERAGKEKGPELDVSREVPRSAE
                     GPEDAGNVSDLIRTPTGSQPGAGSPGPAGGGGAAAIAVWKRRVRGMVEAVWRTPPEIE
                     AVDVTLKTTYLLRVDRSGELIQKRLLVSSGNAPFDRSVLVALGRIQRLPPPPLALIAG
                     GDWVEVTMSFTPPKGAQ"
     gene            complement(1285..1692)
                     /locus_tag="PLT69_00815"
     CDS             complement(1285..1692)
                     /locus_tag="PLT69_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HOJ13022.1"
                     /translation="MQTNKPRTFLAEINVTPFVDVMLVLLIIFMVSAPMMKEGMKVDL
                     PEAEARALGETQDLVITVGPEGTIDINGSKVELERLGMILGQVKEQRNVDAVYLQADR
                     SIPYGFVVKVMSVVRSAGLTRIGLVTQPPVGQR"
     gene            complement(1679..2392)
                     /locus_tag="PLT69_00820"
     CDS             complement(1679..2392)
                     /locus_tag="PLT69_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ13023.1"
                     /translation="MPPFESNAYAAGVQFKGGLATMIVDADPVVKLVLLVLVVFSVFS
                     WGIIIQKWVTLSRARKRAHGILEAAMGASGLAKIKDAATKAGECPLAHVLAEGMDELA
                     RLTARAGAISQPMLENVDARLMNASARQVMALSHGLGFLASISNASPFIGLFGTVWGI
                     MDSFRHIGIMGSANLATVAPGISEALVATAAGLFVAIPATLFYNYFTSLVTTSSGQMD
                     QFRVDLVNLLRRGAFHADK"
     gene            complement(2445..3482)
                     /locus_tag="PLT69_00825"
     CDS             complement(2445..3482)
                     /locus_tag="PLT69_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13024.1"
                     /translation="MEDIVLDVCTPFGYMGSMNLETFREVLELAPVGVLLADEHGGVL
                     WANGAALELLGISWQNLPELNVDGLMDADSPRRETMTRTKGNEHVTLRLMKRTMKNGM
                     SVVFLSDISEVHRLQSEILKMDKLASVGELTSGIAHEIRNPLAGIKTTAQALDRELNP
                     QDPRRAYLSRIIAEIDRLNKLLTGFFDFARPRKLALVSCDLAKVVEAALGMVKAHARE
                     NRVRTLEFYPRAEVAVKADPNLLQQVFMNVFLNAIEAMEFGGRMEVHVLDRGKHVEVV
                     VSDTGRGVPENVQGKIFDPFFTTKPKGVGLGLSISYRIVKQHAGDITFESSPGGTTFI
                     ITLPKDPGKAG"
     gene            3635..4078
                     /gene="mraZ"
                     /locus_tag="PLT69_00830"
     CDS             3635..4078
                     /gene="mraZ"
                     /locus_tag="PLT69_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ13025.1"
                     /translation="MFKGHHVNTINEKGRLSIPSRFRELLNESGADHFVVTKSVDPCL
                     WVFTPEGWERLEKIASGLSTMDRLQMAFIRHVVGSAEDCPVDAQGRILIPMSLRSYAG
                     LKKKCLIVGLINRIEIWNNDTYDVETSGAFEQLRNSREALAAFGL"
     gene            4083..5006
                     /gene="rsmH"
                     /locus_tag="PLT69_00835"
     CDS             4083..5006
                     /gene="rsmH"
                     /locus_tag="PLT69_00835"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ13026.1"
                     /translation="MVHVPVLLKEVIELMAPFDGGRYFDGTLGDGGHASAILEASSPS
                     GELAGCDVDREAVERAGRSLGRYAGRAHLFVGSYTSIDRITSGLGWEGLDGILVDLGV
                     SSRDLLDAQRGFSFTLDGPLDMRYDRSRGVTAHEVVNRYDERELARIIREYGEERFAK
                     RIARRIVEARPIETTATLASVVSEAIPRSSWPRKIHPATKTFQAVRMEVNAELENLSE
                     FLPKAASLLNPGGVLAVISFHSLEDRIVKRFMSPEAGRPFPGRRPPVSPDAAGFALER
                     VTKKPVTASPGEIAANPRARSAKLRAARRVA"
     gene            5003..5293
                     /gene="ftsL"
                     /locus_tag="PLT69_00840"
     CDS             5003..5293
                     /gene="ftsL"
                     /locus_tag="PLT69_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOJ13027.1"
                     /translation="MTSADLKRMLGPLLVMVSSLAFWASVHVWSRHQVIELGYALSRE
                     QARMEQLASQNRALRIEISTLKSPKRLEAIAQKDLGLTPPRPEQVVYLWSNE"
     gene            5278..6984
                     /locus_tag="PLT69_00845"
     CDS             5278..6984
                     /locus_tag="PLT69_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ13028.1"
                     /translation="MVKRVRIIQTVMLATLGILAIRAFEFQVVRSEEIIDRANKKYER
                     IVELTPNRGAIFDRNNQPLAESVPVKSIAANPKAITNPREASARLARILSLDRRSLEK
                     RLSQDKAFTWIKRQVDPSVAKQVKKLGIDGLSFFDEAKRFYPESESMANLIGIVGIDG
                     KGLEGIELSYDSTLRGQDRRITVNKDGKGRIIYARGVAPDNNQDGATLWLTVDRRIQH
                     VAYEMLKKTVVDRNAESGFVIISNPITGEILALASYPSFDPNLTSFRSLAGHTNMAVT
                     HVFEPGSVIKPLWVSWGLEKKLFDLSKSIYCENGAYTFHRVTIHDHEKYGWLGVTDII
                     KYSSNIGIAKLTDQLRASDMYECLSEFGLVDPTGIDFPGEPRASIRNPGSWRAVDKAN
                     ISFGQGFAVTGIQLLTAFNAMINGGMVVRPYLVAKKADAKHKVVTETSSLLLRRAISE
                     QTSAQILGVMKSVTSKGGTGEAAAVPTFEVFGKTGTAQKVDQLTGTYVKGDYVSSFIG
                     GVIDASGRPRVTMMVCINEPRGGYYASVVACPLFRDIVTQCATIMELNPNMRVAGREA
                     GQ"
     gene            6981..8429
                     /locus_tag="PLT69_00850"
     CDS             6981..8429
                     /locus_tag="PLT69_00850"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOJ13029.1"
                     /translation="MRASELAAALRDAVVSGPDVPFGALRADSKRVAPGDAFIALEGS
                     TTDGHLFIGDAVSRGASLVVCNAGRAEDLGGATFVTVPDTKVALKTILGLLHPRAKGV
                     RMIGVTGTNGKTTTTYLVESILKVEGIPCGLIGTIEVRHPQGRLSSTLTTPGPVDLYE
                     LLDGMAASGAQACVMEVSSHALDQDRVLGIAFECAVFTNLSQDHLDYHHDMESYFSAK
                     RRLFTEHLEGTAVVNGDDPYGRRLASEIPQAVEFGFDEHAPIHPVSMENTPEGLSLEL
                     ATPRGEIALKSRLRGAMNAANIMAAVGACQAMGCTNEGISAGIAALTGVPGRMEPVPN
                     RRGLTILVDYAHTPDALDTVLKDAKAFTKGRLIVVFGCGGDRDRTKRPVMGSIAAGRA
                     DLVFVTSDNPRTEDPEAIIGEILAGIPGGSNVHVEPDRAMAIARAVSEMRADDCLVIA
                     GKGHEDYQIVGTTKIPFDDRACVRSFLGEEDR"
     gene            8426..>9798
                     /gene="murF"
                     /locus_tag="PLT69_00855"
     CDS             8426..>9798
                     /gene="murF"
                     /locus_tag="PLT69_00855"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOJ13030.1"
                     /translation="MNGISARDVVAFTDGYLLSGDIDTVFTGVSTDSRSIGPGELFVA
                     LRGERFNGHAYVSQALERGAAGAVVMEEIPRAGGVIISVEDSLQALGDIAAGVRGRHD
                     VAIVGVTGSTGKTTVKELCASILSLAGPCLKTEKNYNNLVGVPLTLLSLRPDHEFAVI
                     EMGTNRFGEIDRLSSITRPLVSIITNINPVHLSGLRSISGIIREKQAIFRNTAKCGCA
                     VINPSLEYMDGVEIPPQLQRITFSHTTKADISLTGIRHRGLDGSDIDVDLAGKPIQVH
                     VPLPGMHNVINALGACACAVALNMDPEVIAEGIRTARFPGMRSEIVISDHLTIINDCY
                     NANPASMKAALSMLAESPHTFKVAVLGDMLELGGDAAFWHEELGRWVAAFGIDRLVCI
                     GEMADVVCSAAVAAGMAGRDVFRVHTKSEILHNLQDVFDKDAMVLVKASRALELDQVV
                     NQLKQVA"
BASE COUNT         1805 a         3257 c         3143 g         1593 t
ORIGIN      
        1 gccgacgccg agtgcccaaa gatggaggac ccgtgcgtgg cggcggagtt gtcgttgttc
       61 gagatatgcc gcgtcggcgg ccgagtaagt cgctttcaag ttggctccca aggaaggcgt
      121 gcacggcgcg gtggggctca cctgcgctcg ccgtgtacag ggtgtcgtcc accacggtgg
      181 cggtggacac ctcaaccatg tggaatctca cgcgcacggc cccgtcctgg atggtgacca
      241 tggccgccac cgcgtagtcc gcgccgatga gcgcgtagtc cttgagcgac tggagggcta
      301 tcctgggtgc gggtgcgggg ttcacgtacg acgcggggtc tatgacgtcg aacaacccgg
      361 tgatggcgag gtccctggcg cacaccttcc agagggactg cgccgtggcg gggtcgccgg
      421 cgaacggcgg catcgcgacc ttgagcctcg cgaacccggg cgcctccacg tcgatgtgca
      481 cctgctgggc cgaacccgtg gcctgcaacg cacaggcaac gaggaacaag aaaaacacga
      541 tcctgctctt tcgggcgatc ttcattgcgc tcctttcggc ggcgtgaaag acatcgtgac
      601 ctccacccag tcgccgcccg cgatgagggc gagaggaggc gggggcagcc tctgtatcct
      661 gcccagggcc acgagcaccg agcggtcgaa cggcgcgttc cccgacgaca ccaaaagcct
      721 cttctggatg agctcccccg agcggtccac cctgagaagg tacgtggtct tcagggtcac
      781 gtccaccgcc tctatctcgg gaggggtcct ccagacggcc tcgaccatcc ccctgacccg
      841 tctcttccac acggcgatgg ccgccgcccc tccaccgcct gcgggccccg gcgagcctgc
      901 gcctggttga gaccccgtgg gggtcctgat gaggtccgac acgttgcccg catcctccgg
      961 gccttcggcc gaccggggga cctcacggga cacgtccagt tccgggccct tctccttccc
     1021 tgccctttcc ttggccacct cgccgatcga gaccgccctg ccggtcctcg agtagacgaa
     1081 cctcttgccg ggcgccccag ccgccgcccc gccgaggtcc gccgcagcct cgcccgaagg
     1141 tcctcccgcg acgatctcca cctcgtagac accctgggag tcggccgagg ggcccgacca
     1201 cacgaacccc aagaacgcca ggaggatcac ggcgtggcac cctaccgaga tggcaaggta
     1261 tctccccatc gcaccccgtc gccttcaccg ctgaccgaca ggcggctgcg tcaccaggcc
     1321 gatcctcgtc aggcccgcgg atctcacgac ggacatgacc ttcaccacga agccgtaggg
     1381 gatcgacctg tccgcctgga ggtacaccgc gtccacgttc ctctgctcct tcacctggcc
     1441 gaggatcatc ccgagacgct cgagctcgac cttggacccg ttgatgtcta tggtcccctc
     1501 gggccccacg gtgatcacga ggtcctgggt ctcgcccagg gccctggcct ccgcctcggg
     1561 gaggtcgacc ttcatgccct ccttcatcat gggcgcggac accatgaaga tgatgaggag
     1621 cacgagcatc acgtcgacga acggcgtgac gttgatttcc gcaaggaagg tcctgggttt
     1681 atttgtctgc atggaaggcc cccctgcgca ggaggttcac gagatcgacc cggaactggt
     1741 ccatctggcc ggaagaggtc gtgacgaggc tcgtgaagta gttgtagaag agcgtggccg
     1801 gtatggccac gaagaggccc gccgctgtgg ccacgagcgc ctcggagatg cccggcgcga
     1861 cggtggcgag gttggcggac cccatgatcc cgatgtgcct gaacgagtcc atgatgcccc
     1921 agaccgttcc gaacagcccg atgaacgggc tcgcgttgct gatgctggcc agaaaaccca
     1981 gaccgtggga cagggccatg acctgcctgg cagacgcgtt catgagcctg gcgtccacgt
     2041 tctcgagcat gggctggctg atggcgcccg ccctggccgt gagcctcgcg agctcgtcca
     2101 tgccctccgc gagcacgtgg gccagggggc actcccctgc cttggtcgcg gcgtccttga
     2161 tcttggccag gccggaagcg cccatcgcgg cttcgaggat gccgtgcgcc ctcttccttg
     2221 cccgcgagag cgtgacccac ttctggatga tgatgcccca cgagaagacg gagaacacga
     2281 cgagaaccag cagcacgagc tttaccacgg gatcggcgtc cacgatcatg gtggcgagcc
     2341 ctcccttgaa ctggactcct gctgcataag cgttcgactc gaaaggcggc atgtcattcc
     2401 tccttgtgct gttcaatacc ttgccaagag acccccgacg cggatcaccc cgccttgccc
     2461 gggtccttgg gcagggtgat gatgaaggtc gtcccgccgg gcgatgactc gaacgtgatg
     2521 tcacccgcat gctgcttgac gatcctgtac gagatggaca gccccaggcc cacgcccttg
     2581 ggcttggtcg tgaagaaggg atcgaagatc ttgccttgca cgttctcggg cacccccctg
     2641 cccgtgtccg agaccaccac ctccacgtgc ttccccctgt cgaggacgtg cacttccatc
     2701 ctcccgccga actccatggc ctctatggcg ttgaggaaca cgttcatgaa cacctgctgg
     2761 aggaggttcg ggtcggcctt cacggcgacc tccgcccggg ggtagaactc gagggtcctc
     2821 accctgttct ctctcgcatg ggccttcacc atgcccaggg cggcctccac gaccttggcg
     2881 aggtcgcagg agacgagggc cagcttgcgc ggcctggcga agtcgaagaa ccccgtgagg
     2941 agcttgttga gcctgtcgat ctccgcgatg atccgcgaga gataggctcg gcgcggatcc
     3001 tgcgggttga gctcgcggtc gagtgcctgg gcggtcgtct tgatgccggc cagcgggttc
     3061 ctgatctcgt gcgctatgcc cgaggtgagt tcgcccacgg aggcgagctt gtccatcttc
     3121 aggatctcgc tctgaagcct gtggacctct gatatgtcgc tcaggaacac caccgacatg
     3181 ccgttcttca tcgtccgctt catgagcctc agcgtgacgt gctcgttccc cttggtgcgc
     3241 gtcatggtct ccctgcgggg ggagtcggcg tccatgagcc cgtccacgtt cagctcggga
     3301 aggttctgcc aggatatgcc caacaactcc agcgcagcgc cgttcgccca caggacccca
     3361 ccatgctcgt ccgcaagcag aacccccacg ggggcgagct ccaacacctc cctgaaggtc
     3421 tccaggttca tggagcccat atagccaaag ggcgtgcaaa catccagtac gatgtcttcc
     3481 atgacacttt tttccacttt cccatgcaca cccattctgc gctatattct gtgcattgac
     3541 cccttccccc accgaccaac ttcatgttga cttcatgagg tgtttcgttt atatgacata
     3601 aagtgtcata ttgtgtcaat gcgaggggat gggggtgttt aaaggtcacc acgtcaacac
     3661 catcaacgag aagggtcggc tgagcatacc gtccaggttc agggaactcc tgaacgagag
     3721 cggggccgac cacttcgtcg tcaccaagag cgtcgacccc tgcctgtggg tgttcacgcc
     3781 cgagggctgg gagaggctcg agaagatagc ctccggcctc tccaccatgg acaggctcca
     3841 gatggccttc atcaggcacg tggtggggtc tgcggaagac tgccccgtgg acgcccaggg
     3901 aaggatcctc atacccatga gcctgagatc gtatgcgggc ctgaagaaga agtgcctgat
     3961 cgtcgggctg atcaaccgca tcgagatctg gaacaacgac acctacgacg tcgaaacgtc
     4021 cggcgcgttc gaacagctca ggaactcgcg cgaggccctg gcggccttcg gcctgtagga
     4081 ccatggtcca tgtccccgtc ctgctcaagg aggtcatcga gctgatggca cccttcgatg
     4141 gaggcaggta tttcgacggc accctggggg atggcggcca cgcctcggcc atcctcgagg
     4201 ccagctcgcc gtccggggag ctggccggct gcgacgtaga ccgcgaagcc gtcgagaggg
     4261 cgggcaggtc gctcggccgg tacgccggca gggcccacct cttcgtcggc agctacacgt
     4321 ccatcgaccg gatcacaagc ggcctgggct gggaaggcct cgacggcata ctcgtcgatc
     4381 tcggcgtgtc cagtcgcgac ctgctcgacg cccaaagggg gttctcgttc accctggacg
     4441 gccccctgga catgaggtac gaccgctcga ggggcgtgac ggcacacgag gtggtgaacc
     4501 gctacgacga gcgcgagctc gcccgcatca tccgtgaata cggggaggaa cgcttcgcca
     4561 agaggatcgc gagaaggatc gtggaggcga ggccgatcga gaccacggcg acgctcgcat
     4621 cggtggtgag cgaggcgatc ccgagatctt cctggccgag gaagatccac cccgccacga
     4681 agacgttcca ggccgtccgc atggaggtga acgcggaact ggagaacttg agcgagttcc
     4741 tccccaaggc ggcgtccctg ctcaacccgg gaggggtgct tgcggtcatc tcgttccatt
     4801 ccctggaaga ccgcatcgtc aagaggttca tgtcccccga ggcgggcagg cctttccccg
     4861 gcaggcggcc gccggtgtca ccggacgccg ccgggttcgc cctcgagcgg gtcacgaaga
     4921 agccggtcac cgcctccccc ggggaaatcg cggcgaatcc ccgcgcccgc agcgcaaagc
     4981 tcagggccgc caggagggtc gcatgacgtc ggcggacctc aagaggatgc tcggccccct
     5041 gctcgtgatg gtctcgtcgc tggccttctg ggcgtcggtg cacgtgtggt ccaggcacca
     5101 ggtgatcgag ctgggctacg cgctcagccg ggaacaggca cgcatggaac agctcgcgag
     5161 ccagaacagg gcgctgcgca tcgagatctc cacgctcaag agcccgaagc ggctcgaggc
     5221 gatcgcgcag aaagacctgg ggctcacgcc ccccaggccg gagcaggtgg tgtacctatg
     5281 gtcaaacgag tgaggatcat ccagaccgtc atgctcgcca ccctggggat cctggcgatc
     5341 cgcgccttcg agttccaggt cgtgcgctcc gaggagatca tcgacagggc gaacaagaag
     5401 tacgagcgca tcgttgagct cacccccaac cgcggggcca tcttcgaccg gaacaaccag
     5461 ccgcttgccg agagcgtccc cgtcaagtcg atcgccgcga accccaaggc catcaccaac
     5521 ccgagggagg catcggcccg cctggcgcgc atcctctccc tcgacaggcg ctcgctcgag
     5581 aagagactct cccaggacaa ggcgttcacg tggatcaaga ggcaggtcga cccgagtgtg
     5641 gcgaagcagg tcaagaagct cggcatcgac ggtctctcct tcttcgacga ggccaagcgc
     5701 ttctaccccg agagcgagtc catggccaac ctcataggca tcgtgggcat cgacgggaaa
     5761 ggcctggagg gcatcgagct ctcctacgac tcgaccctca gggggcagga tcgcaggatc
     5821 accgtgaaca aggacggcaa gggaaggatc atctacgccc gcggcgtggc gcccgacaac
     5881 aaccaggacg gcgcaacgct ctggctcacc gtcgaccgga ggatccagca cgtggcctac
     5941 gagatgctga agaagaccgt cgtcgatcgc aacgcggagt cggggttcgt catcatatcc
     6001 aacccgatca ccggcgagat cctggcgctg gcctcctacc cgagcttcga ccccaacctc
     6061 acgagcttca ggagccttgc cggccacacg aacatggccg taacccacgt gttcgagccg
     6121 ggcagcgtga taaagcccct gtgggtcagc tgggggctgg aaaagaagct gttcgacctc
     6181 tccaagagca tctactgcga aaacggcgcc tacaccttcc acagggtcac catccacgac
     6241 cacgaaaaat acggatggct gggcgtgacc gacatcatca agtacagcag caacatcggc
     6301 atagccaagc tcacggacca gctcagggcg tccgacatgt acgagtgcct gagcgagttc
     6361 ggcctggtcg accccaccgg gatcgacttc cccggcgaac cgagggcatc catccgcaat
     6421 cccggctcct ggagggcggt cgacaaggcg aacatctcct tcggccaggg gttcgcggtc
     6481 accgggatac agctcctcac cgcattcaac gccatgatca acggcgggat ggtggtgagg
     6541 ccctatctcg tcgcgaagaa ggcggacgcc aagcacaagg tcgtcaccga gacgagttcc
     6601 ctgctcctgc gcagggcgat ctccgagcag acgagcgccc agatcctggg ggtcatgaag
     6661 tcggtgacct ccaagggcgg gacgggggaa gcggcagcgg tgccgacctt cgaggtcttc
     6721 ggcaagacag gcacggcgca gaaggtggac cagctcacgg gcacctacgt caagggtgat
     6781 tacgtctcgt cgttcatcgg cggggtcatc gacgcctcgg gcaggccgag ggtgaccatg
     6841 atggtctgca tcaacgaacc gcggggcggc tactacgcga gcgtggtcgc ctgcccgctc
     6901 ttcagggaca tcgtgaccca gtgcgccacc atcatggagc tcaacccgaa catgcgggtc
     6961 gccgggaggg aggccgggca atgagggctt ccgagcttgc agcggcactg agagacgcgg
     7021 tggtctcggg cccggacgta ccgtttggcg cccttcgtgc cgactccaag agggtcgccc
     7081 cgggcgacgc cttcatagcg ctcgagggga gcaccacgga cggccacctg ttcatcggcg
     7141 acgccgtcag ccgcggggcc tcgctcgtgg tatgcaacgc cggccgcgct gaggacctcg
     7201 gaggcgccac cttcgtgacc gtgccggaca cgaaggtggc cctgaagacc atcctcggcc
     7261 tgcttcaccc ccgcgccaag ggggtgagga tgatcggcgt gacgggcacg aacggcaaga
     7321 ccaccaccac ctacctggtg gagtcgatcc tcaaggtgga gggcatcccg tgcggtctca
     7381 tcggcaccat cgaggtgcga cacccgcagg gccgcctgtc ttccacgctc acgacgccgg
     7441 ggccggtgga cctgtacgag ctcctcgacg gcatggcggc ctcgggcgca caggcgtgcg
     7501 tgatggaggt gagctcgcac gcgctcgacc aggacagggt cttgggtatc gcgttcgagt
     7561 gcgcggtgtt cacgaacctc agccaggacc acctcgacta ccaccacgac atggagagct
     7621 acttttcggc caagcgcagg ctcttcaccg agcacctgga agggaccgcg gtcgtcaacg
     7681 gggacgaccc gtacgggaga cggctcgcct cggagatccc ccaggccgtc gaattcgggt
     7741 tcgacgagca tgcccccatc caccccgtct ccatggagaa cacccccgaa ggcctttccc
     7801 tggagctcgc caccccgagg ggcgagatcg cgctgaaaag ccggctcagg ggcgcgatga
     7861 acgcggcgaa catcatggcc gcggtaggcg cgtgccaggc gatggggtgc acgaacgagg
     7921 gcatatccgc gggcatcgcg gcgctgacgg gcgtgcccgg gaggatggaa cccgttccca
     7981 acaggagggg actcaccatc ctcgtcgatt acgcgcacac gcccgatgcg ctcgacacag
     8041 tgctcaagga cgcgaaggcc ttcacgaagg gcaggctcat cgtggtcttc gggtgcgggg
     8101 gcgaccgcga ccgcaccaag aggcctgtca tgggctccat cgccgcaggc agggccgacc
     8161 tggtcttcgt cacctccgac aacccccgca ccgaggaccc cgaggccatc atcggcgaga
     8221 tcctggccgg gatcccaggc ggctcgaacg tccacgtgga gccagaccgc gctatggcca
     8281 tcgcgcgggc cgtctccgag atgcgtgcgg acgactgcct cgtgatcgcg ggcaagggcc
     8341 acgaggacta ccagatcgtg ggaacgacga agatcccctt cgacgaccgc gcgtgcgtga
     8401 ggtcattcct cggggaggaa gatcgatgaa cggcataagc gcaagggacg tcgtcgcctt
     8461 cacggacggg tatctcctct cgggcgacat cgacaccgtg ttcacggggg tgtccaccga
     8521 cagcaggagc atcggcccgg gcgagctctt cgtggcgctc aggggagagc ggttcaacgg
     8581 gcacgcgtac gtgtcgcagg cgctggagag aggggccgcg ggggccgtgg tcatggagga
     8641 gataccgcgt gcgggcgggg tgatcatatc cgtggaagac agcctgcagg ccctcggcga
     8701 catcgcggca ggcgtgaggg ggcgacacga cgtcgccatc gtgggggtca ccggttccac
     8761 gggcaagacc acggtcaagg agctgtgcgc gtccatcctg tcccttgcag ggccgtgcct
     8821 gaagacggag aagaactaca acaacctcgt gggcgtcccc ctgaccctgc tctcgctgag
     8881 gccggaccac gagttcgcgg tcatcgagat ggggacgaac cgcttcggcg agatcgaccg
     8941 cctctcctcc atcacgaggc ccctcgtgtc catcatcacg aacatcaacc ccgtgcacct
     9001 gagcgggctc aggagcatct ccggcatcat cagggagaag caggccatct tcaggaacac
     9061 ggccaagtgc ggctgtgcgg tcatcaaccc ctcgctcgag tacatggacg gggtggagat
     9121 cccgccccag ctccagcgga tcaccttctc gcacaccacg aaggccgaca tctccctcac
     9181 ggggatcagg cacagaggtc tcgacggctc ggacatagac gtcgacctcg cgggcaaacc
     9241 catccaggtc cacgtgccgc tgcctggcat gcacaacgtg atcaacgccc tcggggcctg
     9301 cgcctgcgcg gtcgcgctca acatggaccc cgaggtcata gccgagggga tacgcacggc
     9361 gaggttcccg ggcatgcgct cggagatcgt gatctccgac cacctcacca tcatcaacga
     9421 ctgctacaac gcgaacccgg cctcgatgaa ggcggccctg tccatgctcg ccgagtcgcc
     9481 gcacaccttc aaggtggccg tgctcgggga catgctggaa ctgggcggcg acgcggcatt
     9541 ttggcacgag gagctcggca ggtgggtggc cgcctttggg atagaccgcc tggtgtgcat
     9601 aggcgagatg gcggacgtcg tgtgttccgc cgccgtcgcc gcgggcatgg cgggccggga
     9661 cgtcttcagg gtgcacacca agagcgagat cctccacaac ctccaggacg tgttcgacaa
     9721 ggacgccatg gtcctcgtga aggcctcgcg cgccctcgag ctcgaccagg tggtcaacca
     9781 gctcaagcag gtggcctg
//
LOCUS       DAOSCR010000013         9669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4614_length_9669_cov_4.008321, whole genome shotgun sequence.
ACCESSION   DAOSCR010000013 DAOSCR010000000
VERSION     DAOSCR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_9669_cov_4.008321"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /gene="infB"
                     /locus_tag="PLT69_00860"
     CDS             complement(<1..1907)
                     /gene="infB"
                     /locus_tag="PLT69_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ13031.1"
                     /translation="MTTKKMRVFELAKELSLESKDLLRIAKDLAISADNPMSVLDVHD
                     VERIKKRIERETQKKEEPQVQEVYEEKRVSTTVIRRRVKAAQPPEQVAAQQASPAEQE
                     AATPETPEAREAPEKAPEAKKPARKREAPQKERPLAAGVDTAPPPAKEEAVLTAEVEL
                     PGIVEAPASQAAPEEVRAETEAPPEMVQETAAEKEAAKAKKKEAPVIVPPLGLSPEEL
                     EKKEAPKEIKKKEKKKGKRPLKVVELLDEEVGVEEEILPAKAAVPAAVEPEKADEPAV
                     MEVYTQVDLYGAGRGPAGKRLKKKKDKRGVGPAQPARKKKLRIGDTITVANLAKEMGL
                     KVSEVIKTLMGMGVMASQNQYISSEEAILAASELGFEVEEAKDEIHEEFFAEPVYEEE
                     AYAARPPVVTVMGHVDHGKTSLLDAIRSENVIDTEFGGITQHIGAYQAHCKGRLITFI
                     DTPGHEAFTSMRSRGAQVTDFVVLVVAADDGVMDQTREAINHARAAKIPILVAVNKID
                     KPNANPTRVREQLSELGLVPEEWGGDTIFVDVSAKKHEGIEDLLEMILLQADMMDIKA
                     RKGGPARGVVLESKLDKNRGPMCTLLIQQGVLKKGDIFIIGTKWGKARAMYDFRGNPI
                     EEAQPAAPVEI"
     gene            complement(1904..2422)
                     /locus_tag="PLT69_00865"
     CDS             complement(1904..2422)
                     /locus_tag="PLT69_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOJ13032.1"
                     /translation="MGCYKVSSKSELIRFVTRGPSGVVFDERQTMPGRGFYLCPSREC
                     FMAAPRNKRARGAISSSQAAQWLFHRVLDELAGSLREMLRGCGHAGRHTEGEGSSRVA
                     GAVIVAGAAPGDVDLAPPDREPGPTAPVYTIPKDILGDRPMVVIDDKAPGFKALIRTV
                     RLYERLCSGGRS"
     gene            complement(2445..3698)
                     /gene="nusA"
                     /locus_tag="PLT69_00870"
     CDS             complement(2445..3698)
                     /gene="nusA"
                     /locus_tag="PLT69_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOJ13033.1"
                     /translation="MVLNLSKVIELIVKEKGLRREDLVRVVEEAVQNAAKKVFGDYDN
                     IEAHYNSELDEVEVFQFKVVTDQDDFDPNFEIPLKKARELDPSCQIGDELGEKLDVSK
                     LGRIAAQSAKQVIVQKVKEVERDAIYNEFIGYKGEIVSGFVHRFDRRDAIVSLGKAEA
                     VLEEKEQIPGEVLRRGDRVQALVLDVTKSPKSQHLIKLSRTHPRFLHKLYEMEVPEVA
                     EGVVEIVAIAREPGVRAKIAVRSHDPKVDPVGTCVGVRGARVQNVIQELKGERQDIVV
                     WAEDPVHFVCNALAPARAQKVIVDKAGKSMDVVVAEDQLSLAIGRKGQNVRLVAKLTG
                     WKIDVRSEGSEVVVSSEDKKSIERLADGEIDHSVVEVLMDAGYKTLDDLAKAKEEDLI
                     EFEGIDEALAKKIIERASAVGAGEA"
     gene            complement(3708..4115)
                     /gene="rimP"
                     /locus_tag="PLT69_00875"
     CDS             complement(3708..4115)
                     /gene="rimP"
                     /locus_tag="PLT69_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOJ13034.1"
                     /translation="MLTDLVKPELAMMGFELIELEYAGKTLRIVIDRPGGVSMDDCVS
                     VTRRIGMVLDASDPISSSYRLEVSSPGLTRRIKAPWEYDHFSGRKVRVQTVSGSTVRG
                     TIKGLDGDRVALVVDGVETSLGLDEILKANLDF"
     gene            complement(4219..4294)
                     /locus_tag="PLT69_00880"
     tRNA            complement(4219..4294)
                     /locus_tag="PLT69_00880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4259..4261),aa:Gly,seq:ccc)
     gene            complement(4642..5040)
                     /locus_tag="PLT69_00885"
     CDS             complement(4642..5040)
                     /locus_tag="PLT69_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ13035.1"
                     /translation="MIKRLELKTSARTQLVDITAQVKGILRNENITSGMAFVYVPHTT
                     CGITINENADPSVKDDILRCLERLVPQGDHYRHAEGNADAHIKATLVGSSVALIIEGG
                     APVLGQWQAIYLAEFDGPRKRSVLVKIMTS"
     gene            complement(5126..5926)
                     /locus_tag="PLT69_00890"
     CDS             complement(5126..5926)
                     /locus_tag="PLT69_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ13036.1"
                     /translation="MVDSHNHIHFREFRKDLDEVLGRAVSAGLTAMLTVGIDPKDSKV
                     AVAMASSNELVYASVGIHPQMASRFGPDDVYRLEGLCTPRTLAVGETGFDLYRSPQTR
                     NEQEEIFKAHIVLAKRLGLLLVIHDRDAHDETVAVLDESEGWDAGGVFHCFSGDVELA
                     RKVVENGFLVSIPGVVTYHNADKLKDVVRDIPLEALLVETDAPYLAPVPMRGKRNEPS
                     FVRHAVEEIARIKGVSAQDVARVTSDNFKRVFFKRARGAEGDHERVHS"
     gene            complement(5920..6711)
                     /locus_tag="PLT69_00895"
     CDS             complement(5920..6711)
                     /locus_tag="PLT69_00895"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOJ13037.1"
                     /translation="MSRKEDMLAFAVDLAQKAALMALHGARDMEGVEVSMKSPTEVVT
                     SIDRKLEEFIVESIARRYPGHGIVAEEDTSRGVGQSHTWFVDPLDGTNNFVRGIPFYG
                     TSIALAIDGKPHLGVCALPVTGEIFWAQSGCGAFANGEKIRVSNTAFLKEAIVAVGLP
                     VLQGGHTGPLERLVKLVPGVRGIRRLGSAALDFCLVASGRLDALWEEGLKPWDVAAGA
                     VILSEAGGVVTRVDGSRFDLYKGDVVGGNEAMNRSVLASFGVARW"
     gene            6737..7270
                     /locus_tag="PLT69_00900"
     CDS             6737..7270
                     /locus_tag="PLT69_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13038.1"
                     /translation="MKNVYKIKRALVVPMAIALVISLPVFLDVFRRGYETRYVVMASL
                     LVILFYLFTVNNMIKRVVLDETGISLRGLFGTAKAPFDEIKRVDGIRFGSRQFIALTA
                     RKNLLIPNNFERFSSLVEEIESRVPKETIADGFLHLKEHIVNRKSDITMAWIVVLLLV
                     LCNVLLFFPHGRMFSFD"
     gene            7443..8237
                     /locus_tag="PLT69_00905"
     CDS             7443..8237
                     /locus_tag="PLT69_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13039.1"
                     /translation="MQGKIICISNQKGGVGKTTTAINLGASLAVLGRRVLIVDLDPQA
                     NATTGLGIDPSSCDRHIYHVMIGDCSLEDILTGTKVTGLFLAPSHTDLVGTDVELMDA
                     NERERILLHAFSSMSSLFDYVLIDCPPSLGLLTLNGLAAAHSLLIPVQCEYYALEGLK
                     SLLNTFHLVKKRLNHALCIEGFLLTMFDSRTRLSHEISLNLREHFSSQVFDTVIPRNT
                     RLCEAPSHGLPICLYDITSKGAQHYLMLAREIMNREVSHEDKTSSR"
     gene            8212..9066
                     /locus_tag="PLT69_00910"
     CDS             8212..9066
                     /locus_tag="PLT69_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ13040.1"
                     /translation="MKTRPRLGKGIDALIPADTRATSIADVIELPLGDIRPNPIQPRK
                     NMVGEKLNELAQSIKMHGLISPILVRRVNNRYEIIAGERRYHACRIAGLEKVPAIVKD
                     VSDDTSFKLSLIENLQREDLNPMEEAEAYATLREQFGLTHQEIADSVMKDRSTISNSL
                     RLLGLPEDMKKSLRDGSLTPGHARAILMMESEEDRLNLYRQIISKGMSVREAERRAQL
                     KKTAPRRKASDPYLDGISAHLTDILSTRVICSWTRKHGRITIEVSSKEELERIVSFLS
                     RSSEPMLS"
     gene            9256..>9669
                     /locus_tag="PLT69_00915"
     CDS             9256..>9669
                     /locus_tag="PLT69_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13041.1"
                     /translation="MKWTCSKDIVQEMLSKTQGFTERKTTLSILGHVLLVAGEETLKI
                     RATDLHTSIEVHSPCLVERPGRCAINAKGFLDVVRELPSGDMVFETDDDGSRAHLVLE
                     NKKIILNIMDPDEFPSIDLNVPSSGTHIPVEAMKHL"
BASE COUNT         1805 a         3099 c         2597 g         2168 t
ORIGIN      
        1 atgatttcca cgggcgccgc gggctgagcc tcctcgatgg ggttccccct gaagtcgtac
       61 atggccctgg ccttgcccca cttggtgccg atgatgaaga tgtcgccctt cttgagcacc
      121 ccctgctgga tgaggagggt gcacatcggg cctcggttct tgtcgagctt cgattcgagc
      181 acgacgccgc gggcggggcc acctttcctg gcctttatgt ccatcatgtc cgcctgcagg
      241 agtatcatct cgaggaggtc ctcgatgccc tcgtgcttct tggctgaaac atccacgaag
      301 atggtatccc cgccccactc ctccgggacg aggccgagct cggagagctg ctcccggacc
      361 cgagtgggat tggcgttcgg cttgtcgatc ttgttcaccg cgaccagtat ggggattttg
      421 gccgccctcg cgtggtttat ggcctccctc gtttggtcca tcaccccgtc atcggccgcg
      481 accaccagga caacgaagtc cgtcacctgg gcgcccctcg aacgcatcga ggtgaacgcc
      541 tcgtgccccg gggtgtctat gaacgtaatg agcctgccct tgcaatgcgc ctggtaggcg
      601 ccgatgtgct gggtgatacc gccgaattcg gtatcaatga cgttctccga tcggatggca
      661 tcgagaagcg aggtcttccc gtggtcgacg tgccccatga cggtgacgac gggcggcctg
      721 gccgcatagg cctcttcttc gtagacgggt tccgcgaaga attcctcgtg gatctcgtct
      781 ttggcttctt ctacctcgaa gcccagctcg gacgccgcca ggatggcctc ctcggaggat
      841 atgtactggt tctggctcgc cattaccccc atgcccatga gggtcttgat cacctccgac
      901 accttcaggc ccatctcctt ggcgaggttc gcaaccgtga tggtgtcacc tatgcgaagc
      961 ttcttcttcc tcgccggttg agctggccca accccgcgct tgtccttctt cttcttcagg
     1021 cgttttcccg caggacccct ccctgcaccg tagaggtcca cctgggtgta aacctccatg
     1081 acggccggct cgtccgcctt ttccggctcg acagccgccg gcacggcggc ctttgcaggc
     1141 aggatctcct cctcgacccc cacttcctca tcgaggagct caaccacctt gagaggcctc
     1201 ttcccctttt tcttttcttt tttctttatc tctttcgggg cttccttctt ctcgagctcc
     1261 tcgggcgaca gtccgagggg tgggacgatg acgggcgctt ccttcttctt cgcctttgcc
     1321 gcctccttct ctgcagcagt ctcctggacc atctcggggg gcgcctcggt ctcagcccgc
     1381 acctcctcgg gcgcggcctg agaggccggg gcctcgacga taccgggcag ctccacctcc
     1441 gccgtcaaaa cggcttcctc ctttgccgga ggaggcgctg tatccactcc ggcagccagg
     1501 ggcctctcct tttgaggggc ctcccgcttg cgcgcgggct tcttcgcttc cggggccttc
     1561 tccggggcct cgcgggcttc cggggtttcc ggcgtggccg cttcttgctc ggccggggat
     1621 gcctgttgcg ctgcgacctg ttcagggggc tgcgcggcct tcaccctgcg ccggatcacg
     1681 gtcgtgctca cgcgcttctc ttcgtacacc tcctggacct gcggttcttc tttcttttgg
     1741 gtctccctct ctatcctctt cttgatccgc tccacgtcat ggacatcgag cacgctcatg
     1801 gggttgtcgg ccgatatggc gaggtccttg gcaatacgca ggaggtcctt ggactccagg
     1861 gaaagctctt tggccagttc aaaaaccctc atcttcttcg tcgtcatgat cgccccccgg
     1921 aacacaaacg ctcgtacagt cgaactgtcc tgatcaacgc cttgaatccc ggcgccttgt
     1981 cgtcaataac caccataggc cgatccccca ggatgtcttt cgggatcgtg tacaccgggg
     2041 ccgtggggcc tggctcacga tcaggcggcg ccaggtccac gtctccaggg gctgccccgg
     2101 cgacgatgac ggccccggcg accctggacg atccctctcc ctcggtatgc ctccccgcgt
     2161 gcccacaccc gcggagcatc tccctgaggg agccggcaag ctcgtccagg acacggtgaa
     2221 agagccattg cgcggcttgg ctcgaggaga tggcgcccct ggctcgcttg tttcgcggcg
     2281 ctgccatgaa gcactccctg gatggacaga gatagaaacc cctccctggc atcgtttgcc
     2341 gttcgtcgaa cacgacccct gacgggcctc gggtcacgaa cctgatgagc tcgcttttcg
     2401 aagacacctt gtagcacccc acacactgtc tgatgggtcc ggatctacgc ctcacctgcc
     2461 cccaccgcgc tcgcacgttc gatgatcttt ttcgcaaggg cctcgtcgat cccctcgaac
     2521 tctatcagat cctcttcctt cgccttcgcg agatcgtcca aggtcttata ccctgcatcc
     2581 atgaggacct ccaccacgct gtgatcgatc tcgccgtcgg ccaggcgctc tatggatttc
     2641 ttgtcctcgg aagagaccac cacttcagac ccttccgacc gcacgtcgat cttccagccc
     2701 gtcagcttgg caacgagccg cacgttctgc cccttcctgc cgatggcgag gctgagctgg
     2761 tcctccgcca ccaccacgtc catgctcttg cctgccttgt ccacgatgac cttctgggcg
     2821 cgtgccgggg ccaacgcgtt gcacacgaag tggaccggat cctcggccca cacgacgata
     2881 tcctgccgct cccccttgag ctcctggatc acgttctgga ccctggcccc cctcactccc
     2941 acacaggtgc ccaccgggtc aaccttgggg tcgtgcgaac ggaccgcgat cttggccctc
     3001 acgcccggct cgcgcgcgat ggccacgatt tccacaacac cctcggccac ctcggggacc
     3061 tccatctcgt acagtttgtg gaggaacctg ggatgtgtcc tcgaaagctt gatcaggtgt
     3121 tgcgatttgg gggacttggt cacatccagg accaaggcct gtacgcggtc tcccctgcgg
     3181 agcacctcgc cagggatctg ctctttttcc tccaggacgg cctcggcctt gcccaggctc
     3241 acgatcgcat ccctcctgtc gaaccgatgg acgaacccgg agacgatctc gcccttgtag
     3301 ccgatgaact cgttgtagat ggcgtccctc tcaacctcct tgaccttttg cacgatcacc
     3361 tgcttggcgc tctgtgccgc tatcctcccg agcttggaga catcgagctt ctccccgagc
     3421 tcgtccccga tttggcacga tggatcgagc tccctggcct tcttgagggg gatctcgaaa
     3481 ttcggatcga agtcgtcctg gtcggtgacg accttgaact ggaagacttc gacctcgtcc
     3541 agctccgagt tgtagtgggc ctcgatgttg tcgtaatccc cgaatacctt cttggcagcg
     3601 ttctgtaccg cctcttccac aactcttacg aggtcttctc tgcgaagccc cttctctttg
     3661 acgatgagct cgatgacttt cgataggttc agaaccatac ccactcccta gaaatcaaga
     3721 ttggccttga gtatctcgtc aagccccaag gaggtttcca ctccgtcgac caccaatgcg
     3781 acccgatccc catcgaggcc cttgatggta cccctcaccg tgcttcccga aacggtctgc
     3841 accctgacct ttcttcctga gaaatgatcg tactcccaag gggccttgat cctcctggta
     3901 agacccggcg aagacacctc gagcctgtag gaggaggata tcgggtcgct ggcgtcgagg
     3961 accatgccga ttctcctggt gacagacacg cagtcgtcca tggacacccc gcccgggcgg
     4021 tctatgacga tccggagcgt cttgcctgca tactcgagct cgatgagctc gaagcccatc
     4081 atggccaact ccggcttcac caagtcggtg agcatcgcga tcatttctct ttccgacatg
     4141 ttttacgaaa caaaaaagtg ggcaattgcc cacttctccg cctccttctc ctcacgtttt
     4201 acgtgccttg tgacacgctg gagcgggcaa cgggattcga acccgcgaca ttcagcttgg
     4261 gaagctgaca ctctaccaac tgagctatgc ccgcactgaa tccatgtaat agatcacgtt
     4321 ttctcgtctt gtcaaggcgc tcttctgtgc ggggaaggaa caggccctgt ctcggtcaaa
     4381 aggaaaaggg ccggttctcc gcggtcttca tcacggtacc cgggggcgca ccgtgggcag
     4441 ggtccggatc gatcagggcg gtccgtcacg ccgtgcgtac gcgttgaatg cctttcgtgt
     4501 tcgagcacct catccgtgcc cctcctgcgc gatggatcag ggcaagagac gctcctcgac
     4561 gtgaagacgc acgccattga tcgtttccgg cgtttcccgt cttccgggcg atcgcgtcga
     4621 gaaggcgggc gcgccagggg ttcaactcgt catgatcttc acgagaacgg atctctttcg
     4681 ggggccgtcg aattccgcca ggtagatagc ctgccactgg cccaagacgg gcgctccgcc
     4741 ctcgatgatg agggcgacgc tcgaaccgac aagggtcgcc ttgatgtggg catcggcgtt
     4801 tccctctgcg tgacggtaat ggtcgccctg gggaacgagt ctttccagac agcgcaggat
     4861 atcgtccttc acgcttggat cagcgttttc gttgatggtg atgccgcagg tggtgtgcgg
     4921 cacgtagacg aatgccatac cagaggtgat gttctcgttg cggagtatcc ccttgacctg
     4981 ggccgtgatg tcaacgagct gcgtgcgcgc cgacgtcttg agttcgagtc gcttgatcat
     5041 ggcctttccc tccagcataa gagacggcga tcacacgaca gggtcatgca acggccatcc
     5101 ctgcctgcgg tcgcaccatg gatcttcagg agtgcaccct ctcatggtcg ccttctgcac
     5161 ctcttgcgcg tttgaagaaa acccttttga aattatcgga agttacacgt gcaacgtctt
     5221 gggccgagac gcccttgatc ctggcgatct cctcaaccgc gtgccttacg aaagaaggct
     5281 cgttcctctt tcctctcatg ggtaccgggg caaggtatgg ggcgtccgtc tccacgagca
     5341 aggcctccaa gggtatgtca cgcacgacat ccttgagctt gtcggcgtta tgataggtga
     5401 cgacgcctgg gatcgacacg aggaaaccgt tttccaccac cttcctggca agctcgacgt
     5461 caccggagaa gcagtggaaa accccgccgg catcccatcc ttcagactcg tcgagcaccg
     5521 ccacggtctc gtcgtgtgcg tcgcgatcat ggatgacgag cagaaggccg agccttttcg
     5581 cgaggacgat gtgggccttg aagatctctt cctgttcgtt tctggtctgg ggtgagcgat
     5641 agagatcgaa ccccgtctcc cctacggcga gggtcctggg agtgcagaga ccctcgagcc
     5701 ggtacacgtc atccggccca aaacgcgatg ccatctgagg atgaataccc acggaggcat
     5761 agacgagctc gttcgacgaa gccatggcta ccgccacctt gctgtctttc gggtctatcc
     5821 ctacggtgag cattgccgta agaccggcgc tcaccgcccg tccaaggacc tcgtcgagat
     5881 ccttcctgaa ctccctgaaa tgaatgtgat tgtgagaatc taccatcgtg cgacgccgaa
     5941 cgatgcaagg accgacctgt tcatggcctc gtttccgcca accacatccc ccttgtacag
     6001 gtcaaaccgg cttccgtcaa cgcgggtaac gacgccgcct gcctccgaga ggatcacggc
     6061 tccggccgct acatcccatg gcttgagccc ctcctcccac aaggcgtcga gccttccgct
     6121 tgcgaccagg cagaagtcaa gcgccgctga tccgagcctc ctgatcccgc ggaccccggg
     6181 aaccagcttg accagacgtt cgagtggccc ggtgtgaccg ccctggagca cgggaagacc
     6241 caccgccacg atcgcctcct tgaggaaggc ggtgttcgac acccgtatct tctctccgtt
     6301 cgcgaaggcc ccgcaccctg attgcgccca gaagatctct ccagtgacag gaagggcgca
     6361 taccccgagg tgtggcttcc cgtcgatcgc aagggcgatc gacgtaccat agaagggtat
     6421 cccgcgcacg aaattgttgg tcccgtcgag gggatcgacg aaccacgtgt ggctttgacc
     6481 cactcccctt gacgtgtcct cttctgccac gatcccgtgc ccgggatatc tgcgggcaat
     6541 gctctcgacg atgaactcct ccagcttcct gtcgatgctc gtgaccacct ccgtgggcga
     6601 cttcatggag acctcgacgc cttccatgtc ccgtgcgccg tggagtgcca tcagggcggc
     6661 cttctgtgcg agatcgacag cgaaagccag catgtcttcc ttgcgtgaca tgaccgtttg
     6721 tgtcatcatg gaatagatga aaaacgttta caagatcaaa agggcgctcg tcgtccccat
     6781 ggcgatcgcc ctcgtcattt cccttcccgt tttcctcgat gtctttcgca ggggttacga
     6841 aacgaggtac gtggtcatgg cctcgctcct cgtcatcctc ttctacctgt tcacggtgaa
     6901 caacatgatc aagcgggtcg tactcgatga aacgggcata tccctacggg ggctcttcgg
     6961 gacggcgaaa gcgccctttg acgagatcaa gagggtggac ggcatacgtt ttggctcgcg
     7021 tcagttcata gctctcaccg cccggaaaaa cctcctgata cctaacaact tcgagaggtt
     7081 ttcctccctc gttgaggaga tcgagtctcg cgtacccaaa gagacgatcg cagatgggtt
     7141 cttgcacctc aaggaacaca tcgtgaatcg aaaaagcgac ataaccatgg cctggatcgt
     7201 cgtcctgctc ctcgttttgt gtaacgttct cctgttcttc ccgcatggga gaatgttctc
     7261 atttgattaa cgtcctgcgc atcctgatcg ccggcacctt tcatgcatca tgaaccagct
     7321 tctgtggccc ctaaagacca tttccgtgac acatggctta taagtcattg atttctttcc
     7381 atttttgttt cacgtgaaac tggttattca ccttttgctc ctcttgtgtt agtggtccgg
     7441 ttgtgcaggg caagatcatc tgtatctcga atcagaaggg gggagtggga aagaccacca
     7501 ctgccatcaa tctcggggcg tccctggccg ttctcgggag gagggtcctc atcgtggatc
     7561 tggatccaca ggcgaacgcc accacgggtc ttgggatcga tccttcatcc tgtgatcgtc
     7621 acatctatca cgtgatgatc ggcgattgct cgctcgagga tatcctgacc ggcacaaagg
     7681 tcaccggcct cttcctggcc cccagccata ccgacctcgt ggggacggat gtagagctca
     7741 tggacgcaaa cgagcgggaa cgcatcttgt tgcatgcctt ctcttcgatg tcttccctct
     7801 tcgattacgt cctcatagac tgtcctcctt ccttggggct cctgacgctc aacggtcttg
     7861 ccgcggccca ctcgctcctc atcccggttc aatgcgaata ctacgcgctc gagggcctca
     7921 agagccttct caacacgttc catctcgtca agaagcgtct caatcatgcg ctctgtatcg
     7981 agggcttcct cttgaccatg ttcgactcgc gaactcgact cagccacgag atatccttga
     8041 atctccgtga acacttctct tcccaggtat tcgacaccgt gatcccacgg aatacaagat
     8101 tgtgcgaggc cccgagtcac ggtctcccca tttgcctgta cgatatcacg tcgaagggtg
     8161 cgcagcacta cctgatgctc gcaagggaaa tcatgaacag ggaggtttcc catgaagaca
     8221 agacctcgtc tcggtaaggg gatagacgcc ctcatccctg cggataccag ggcaacgtcc
     8281 atagcggacg ttatagagct tcccttggga gacattcgcc ccaatcccat tcaaccgcgg
     8341 aagaacatgg ttggtgagaa gttgaacgag ctcgcccagt ccatcaagat gcacggtctc
     8401 atttccccta tcctggtaag acgggtgaac aaccgctacg agatcatcgc gggcgagcgc
     8461 aggtatcacg cttgccgcat cgccgggctc gagaaggtac ctgccatagt caaggacgtg
     8521 tccgacgata catcgttcaa gctctccctc atagagaacc tccagcgtga ggacctgaat
     8581 cccatggaag aggcggaagc gtacgcgacc ctcagggaac agttcggctt gacacaccag
     8641 gaaatcgccg actcggtcat gaaggatagg tccaccataa gcaattctct caggctcctg
     8701 ggccttcccg aggacatgaa gaagtccctg agggatgggt ccctcacccc tggtcatgcg
     8761 agggccatcc tcatgatgga aagcgaggag gaccggctca acctctatcg ccagatcata
     8821 tcgaaaggca tgagcgtcag ggaggcagag aggagggccc aactcaaaaa gaccgccccc
     8881 aggcgcaagg cctccgaccc ttatctcgac ggcatctctg ctcaccttac cgacatcctc
     8941 tccacccggg tcatctgttc ctggacgaga aagcacggca ggatcaccat agaggtctct
     9001 tccaaagagg aactggaacg gatcgtatcc tttctgtcgc gttccagcga accgatgctc
     9061 tcgtgaggtc acctacatgg ttatgaacac cttctctaca tgtcgacagg accgcttccc
     9121 ccgacacgat ccgctccttg tttccgacat gcctccacag tatggatgaa cagtatgttc
     9181 ggtgggttgg gagagttgtg tacatatcga cacgttcttt ctattctatg gaaggtttca
     9241 tcaggaggat aaaagatgaa atggacatgc tcgaaggaca tagttcagga aatgctttcc
     9301 aagacccaag ggtttaccga gcgcaagacc acgctttcca tactcggtca tgtcctcctc
     9361 gtggcaggtg aagaaaccct gaagatcagg gccacggatc ttcacacatc gatagaggta
     9421 cactctccat gcctcgtgga aaggccggga aggtgcgcca ttaatgcgaa ggggttcctg
     9481 gatgtggtaa gggaactccc ttccggggac atggtattcg aaaccgacga cgatgggtca
     9541 agggcgcacc tggttctcga gaacaagaag atcattctca acatcatgga tcccgacgag
     9601 ttccccagca tcgatctcaa cgtaccctcc tccggcacgc acatccccgt ggaggccatg
     9661 aaacatctc
//
LOCUS       DAOSCR010000014         9665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4616_length_9665_cov_3.525702, whole genome shotgun sequence.
ACCESSION   DAOSCR010000014 DAOSCR010000000
VERSION     DAOSCR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4616_length_9665_cov_3.525702"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLT69_00920"
     CDS             complement(<1..1215)
                     /locus_tag="PLT69_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOJ13042.1"
                     /translation="MRTRPTVTAAFILATLVTVVFSVQGVQAHEIPHTVRPGDTLASI
                     AASYVELADAYTKKEFIEEIKRVNNLGDRGLSVGMTLAIPVVRSEPLRPSSVKKPRDF
                     VAKGAYINQFTAGTRQVFTVARRLKACGGNTIVFDAKEVEGLPTYRSAVPREHLGLSS
                     YAYTIEDIHKLVEYLHAMGMHVVARVCMFRDFYNSTNRAAWRFADEWVNPANPAVQEY
                     NLAIVRELLDSGVDEIQVDYFRYPADGKTQTGLEGKSRSDVLCDFARRLRGLTQAKGV
                     VLSLDMFGIVIWQKKEDILILGQDVVRFKDHFDVISPMLYPSHFVKNFSGVANPADEP
                     YLFVNRGVKRMKALVGDAVVIRPWLQSFPLRVKIGYNAAYVRNQIHATCDAGGTGWLL
                     WSPKNRYEEAFRA"
     gene            complement(1351..2220)
                     /locus_tag="PLT69_00925"
     CDS             complement(1351..2220)
                     /locus_tag="PLT69_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOJ13043.1"
                     /translation="MAENKEKVIICAALAGALTFKHQNPAVPYTPEEFAREAARCFAA
                     GAAMVHVHARDDSGMPTHDIDRVRAIHDAIKEKTPELIVNLTSAVGVGVKAEERIAQI
                     LAVKPAMASLNTNTMNFSLVDRSTGAILYDAVFENTFTMLQDFGRAMEENDIKPEIEC
                     YDMGGIDNTLLVAKQGFFSKPMNFNFVWGVAGGQRFRPEAFIAMVHALPEGSTFTTCG
                     VGIDQFPAVTMSCLMGGHMRVGLEDNIRLPNGELAKGSWEQVLWAVKIASALGREPAG
                     PDEARRILGIRQK"
     gene            complement(2289..2885)
                     /locus_tag="PLT69_00930"
     CDS             complement(2289..2885)
                     /locus_tag="PLT69_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13044.1"
                     /translation="MARNMKQAMIIDAALEVFREKGYANARMADIARRAGVSYGLVYH
                     YFGSKEVLFDIIVESWWNDLYAMMEREIASDAPTRDKLVHIIAFFLDTYGSKPNLMSI
                     FVSEVCRSSVYHTKEGLSRFLKFFNLCEQIMLQGQESGFLSKEIPPHHLTYIFYGAIE
                     TFISVMVLGKEPMTKKRHERAMNAILRVFTDGASSRAH"
     gene            3350..3808
                     /locus_tag="PLT69_00935"
     CDS             3350..3808
                     /locus_tag="PLT69_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOJ13045.1"
                     /translation="MKDGLRDVTASARIRIDREGRWYYNDAEIVNPMVLRTFFNALTR
                     DDHGRYCLVIEPEICYVEVEDTPLVVSCIRGEPDTGLFLRLNNLETYPLDPSRLSIGD
                     ANVLYYTLPDGMKVRFSRQAYYSLALMMEELDGSIVLTIAGKPHPICPAE"
     gene            3912..4406
                     /locus_tag="PLT69_00940"
     CDS             3912..4406
                     /locus_tag="PLT69_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ13046.1"
                     /translation="MRRLLWLSVCLLVCVLVVGFLPQAAKADDEAAKKELVSAVNYAV
                     GVIEAKGKAALDELKKYRFGEGNYVYVTDMGAVVIMHPVAPELLNKDCTQIKDAKGKF
                     FGAEMKGKAEKYGSGWTSYWWPNPKKNNAPDLKCAFFKTANMAGTKVIVYAAAFGISE
                     AGCQ"
     gene            complement(4530..5900)
                     /gene="fliD"
                     /locus_tag="PLT69_00945"
     CDS             complement(4530..5900)
                     /gene="fliD"
                     /locus_tag="PLT69_00945"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ13047.1"
                     /translation="MATYISGLTSGLDWQSVISDLMAIERRPITLLEERKTALEAQKS
                     AWREVNTKLNSLMSAAQALSSSDDFEVYSGQAAISGSSKSVSDILDYAVGTNASQGSY
                     SITVNRLAQAQKFASAAFASDDTELGISGTVTLNGRDLTIDATDTLEDIRDKINALNS
                     GDDPAGVTASIIKVADDEYRLTVASKETGASGIDIVDGSGVFSSGLGMNETVSGQDAE
                     IVVEGFTITRSSNLISDVIAGVTLNLKGADESATITLTVSRDNSGVKDKIESFVSAYN
                     DLMSYIDDQNTPAGDTEKPLYADSSLAQLKKTIRNAILSGVDGLSSTLDHLSLIGVNI
                     DKYGKLSIDDDTLEGYLETNFEDVVSLFSADGLTYETTGVASRLEEAIDAMTDSIDGY
                     VVTRQESLQSQMDKLDRKIEDMEERLERKQKALESKFVAMETMLSTLQAQQDWLSSQI
                     STLVSK"
     gene            complement(5970..6350)
                     /locus_tag="PLT69_00950"
     CDS             complement(5970..6350)
                     /locus_tag="PLT69_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOJ13048.1"
                     /translation="MAMKIESLTVMPGDRLSRDGPVPQPVAVSKPRIPDSDSDSQAAT
                     KPLQKDDRSADEMKKDIEAINEQLAVLNQSLQFRIDEESKDVVIRIVDKDSGEVIKQI
                     PPEEIMNLRRRLREMSSLFVERTV"
     gene            complement(6411..7241)
                     /locus_tag="PLT69_00955"
     CDS             complement(6411..7241)
                     /locus_tag="PLT69_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOJ13049.1"
                     /translation="MALRINTNVAAINAHRQLLVTEAGMNKSMERLSSGYRINKAADD
                     AAGLAIANRLRANVKSLTVASRNVTEGKALLQIAEGSANQIESILERMKELAVQASSA
                     NAGTDRDKLDAEFQSLIDEIDRIVDDTNYQGTKLLDGNFDGTFQVGAMNAADSSGSSQ
                     INIAISGLDTTTLGISTAAVDSAAHADSAIGLIDDAINTLAGVLGDIGATMNRLDYTY
                     SNLQVQIENFSAAESVIRDVDMASEMVTFTKSQILLQAGTAMLAQANMSSQVVLSLFG
                     "
     gene            7795..>9665
                     /locus_tag="PLT69_00960"
     CDS             7795..>9665
                     /locus_tag="PLT69_00960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ13050.1"
                     /translation="MERPLVTIACTTYNHEKYIGQTIDAFLEQRTTFPVEIIINDDAS
                     TDNTPRIIREYAENHPGLIVPIFQAENQYSKGRKPFIEFVLPRARGTYIALCEGDDYW
                     TDPAKLQKQVDFLEANPDYVLCYHNARIVDEYGALVSASKLPDDLKRDLSAEELIRGA
                     MILTLTLCFRNIVKEIPEELFKVYNGDKFFTSLLGRHGKGKYMDDITDAVYRKHSDSI
                     WSSLDPVRQIYHNGVTRAWMHRYYKRIGETHHADYFRDQVIMHFTRTLQEIAARTGKA
                     YDDIVEDIFSNFTDIVDQASQERLRSLMTPARQVIHSTAATASSAAHLTAGQSGKTGA
                     ARLSDVPPDGEYMSCTYIENAIAFYYDNLVYTCCYPGYIQDEGILADLSRGPLNTADI
                     IARKREIIRRRMNKEFGGCAGCPRFVKKRWRRNWDISISHVNLNHLLACNLKCLHCGY
                     VEKVGRHQPTSKESIMDAVATLQEGGLLEPGAEFYIGNGEPSINADMDDLIAFLLDRN
                     YSVVVQSNGTVYKDIYAAGVNAGKLRLILTPDAGTRDSYLKIKGRDYFHRVWRNIRKY
                     ASETSGNITVKLILQEGNINDAEPFLDLCDSARVRDIVVDVDLHIRDRISKEIAAAA"
BASE COUNT         1788 a         2964 c         2875 g         2038 t
ORIGIN      
        1 ggccctgaac gcctcttcgt agcggttctt cgggctccag aggagccatc ccgtcccgcc
       61 tgcgtcgcac gtggcatgta tctggtttct cacgtacgcg gcgttgtacc cgatcttcac
      121 cctcagcggg aacgactgca gccacggcct gatgaccacc gcatccccca ccagggcctt
      181 catccgtttg acccccctgt tcacgaacag gtacggctcg tcagcggggt tggccacccc
      241 ggagaagttc ttgacgaagt gcgacgggta gagcatgggc gagatcacgt cgaagtggtc
      301 cttgaaccgc accacgtcct ggccgaggat gagtatgtct tctttcttct gccagatcac
      361 gatcccgaac atgtcgagag acagcacgac gcccttggcc tgggtgaggc cgcgaagtcg
      421 cctggcgaag tcgcagagca cgtcgctgcg cgactttcct tccaggcccg tctgtgtctt
      481 gccgtccgca gggtagcgga agtagtccac ctgtatctcg tcgacgcccg agtccagcag
      541 ttccctgacg atggcgaggt tgtactcctg caccgccggg ttcgcggggt tcacccactc
      601 gtcagcgaac ctccatgcag ccctgttcgt ggagttgtag aaatcccgga acatgcacac
      661 gcgtgccacc acgtgcatcc ccatggcgtg gaggtactcc accagcttgt ggatgtcttc
      721 tatggtgtat gcgtaggatg acagccccag gtgttccctc gggacggcgc tccggtaggt
      781 ggggagcccc tccacctcct tggcgtcgaa cacgatggtg ttgccgccgc acgccttgag
      841 cctcctggcg acggtgaaga cctgcctcgt tccggccgtg aactggttga tgtatgcgcc
      901 cttggccacg aagtcccgtg gcttcttgac gctcgacggc ctgagcggct cactgcggac
      961 caccggtatg gccagggtca tgcccacgct gagccccctg tcaccgaggt tgttgacccg
     1021 cttgatctcc tcgatgaact ctttcttcgt gtacgcatcc gcgagctcta cgtacgaggc
     1081 ggcgatcgat gccagcgtgt cgcccggcct gaccgtgtgg gggatctcgt gggcctgcac
     1141 cccctgcacg gagaagacca ccgtcacgag ggtcgccagg atgaacgccg ctgtcaccgt
     1201 cggccgtgtc cgcatatcac tccacctcgt ccattgccgg atacgcgcga ccccgccaaa
     1261 gacccgtgtc ggcgaccccg cacatcctcc ctctgcccat ggggccgccg tccgagagcc
     1321 gagccgcaag aagatcctcc gcatgagcgc tcacttctgc ctgatgccca ggatgcgccg
     1381 ggcctcgtcg ggtcctgcag gctccctgcc gagcgcggac gcgatcttga ccgcccacag
     1441 gacctgctcc cagctgccct tcgcgagttc gccgttggga agccgtatgt tgtcctccag
     1501 gcccacgcgc atgtgccccc ccatgaggca gctcatggtg acggccggga actggtcgat
     1561 ccccaccccg caggtcgtga aggtcgagcc ttcaggaagg gcgtgcacca tcgctatgaa
     1621 cgcctcgggc ctgaaacgct ggccgcccgc cacgccccag acgaagttga agttcatcgg
     1681 cttggagaag aacccctgct tcgcgaccaa gagcgtgttg tctataccgc ccatgtcgta
     1741 gcattcgatc tcgggcttga tgtcgttctc ctccatggcc ctgccgaagt cctggagcat
     1801 ggtgaaggtg ttctcgaaga ccgcgtcgta gaggatcgcg ccggtcgacc ggtcgacgag
     1861 gctgaagttc atggtgttgg tgttgagcga tgccatggcg ggcttcaccg caaggatctg
     1921 ggcgatcctc tcctcggcct tcacccccac ccccacggcc gaggtgaggt tcacgatgag
     1981 ctccggggtc ttctccttga tggcgtcgtg gatcgccctc acgcggtcga tgtcgtgggt
     2041 aggcatgccg gagtcgtccc gcgcatggac gtgcaccatg gcggcccccg ctgcgaaaca
     2101 ccttgccgcc tcccgggcga attcctcggg cgtgtagggc accgccgggt tctggtgctt
     2161 gaaggtcagg gcgcctgcga gggcggcgca tatgatcacc ttttccttgt tttcagccat
     2221 ggcattcctc ttcggccgcc cccctcgatg acctgaaggg gtggacggcc cctcccggcc
     2281 aaaaaggcct agtgcgccct ggaactggca ccatcggtga agaccctgag gatggcgttc
     2341 atggcgcgct cgtgacgctt cttcgtcatg ggctccttgc cgagcaccat gaccgagatg
     2401 aaggtctcga tggcgccgta gaagatgtac gtgaggtggt gcggcgggat ttccttgctc
     2461 aagaacccgc tctcctgccc ctggagcatg atctgctcgc agaggttgaa gaacttgagg
     2521 aacctcgaga gcccttcctt ggtgtggtac accgagctcc tgcatacctc gctgacgaag
     2581 atggacatga ggttcggctt gctgccgtag gtgtcgagga agaaggcgat gatatggacg
     2641 agcttgtccc tcgtgggcgc atccgacgcg atctcccgct ccatcatggc gtagaggtcg
     2701 ttccaccagg attccacgat gatgtcgaac agcacctcct tgctcccgaa gtagtggtac
     2761 acgaggccgt aggacacgcc ggcgcggcgc gcgatgtccg ccatgcgcgc gttcgcgtac
     2821 cccttctcgc ggaagacctc cagggccgcg tcgatgatca tcgcctgctt catgtttcga
     2881 gccatagaca acccttcgat ctcggtatgg tgccaagtat tctgccttgg atgctcgcgc
     2941 ctcgtcaagc ccggaatcga ctcgcggatc gagagcctcg acccgcaggc tgcctgtagg
     3001 ttgcgtcggg ccgctctcga cccggcaacc gggagggttg tctccaaaga ccgctgcctc
     3061 tcgcgcgtcc acgacccggc tcggttcaag acgaccctgt ctgcggggtc gtatacctgt
     3121 tcggcgcgga gctgacccga tccttccaca aacctggtcg atttccgtcc acgacgagaa
     3181 ggtcaccctt gcagggcggg gttttaaggc tggacagccc ctgccgggct gcagcatgga
     3241 aggcgcgggt cacgccggag cccgcccctg cggcagggga tgcatttccc gcgtccacgt
     3301 gctatcatct catggaacac catcgagcgg attcacgaac ggagacgaca tgaaagacgg
     3361 cttgagagac gtgacggcat ccgcgaggat ccggatagac agggaaggcc gctggtacta
     3421 caacgacgcg gagatcgtca accccatggt cctcaggacc ttcttcaacg cgctgacgag
     3481 ggatgatcac gggaggtact gcctcgtcat cgagccggag atctgctatg tggaggtgga
     3541 ggacacgccc ctggtggtct cgtgcatcag gggggagccc gacacagggc tcttcctcag
     3601 gctcaacaac ctcgagacgt atcccctgga cccgagccgc ctgtccatag gggatgcaaa
     3661 cgtcctgtac tacacgctgc ccgacggcat gaaggtgcgc ttctcgagac aggcgtacta
     3721 cagcctggcc ctcatgatgg aggagctaga cggctcgatc gtcctcacca tcgcgggcaa
     3781 gccccacccc atctgccctg ccgagtgaac catcacccat catatccata cgttatcacg
     3841 ttcattattc ggtattgata ttcggcctca ttcatccgat atacacggga aacttacccc
     3901 ggaggtgtat catgagaaga ctcctttggt tgtccgtctg tctccttgtc tgtgtactgg
     3961 ttgttgggtt cttgccccag gcggcgaagg ccgacgacga ggcggcgaag aaagagctcg
     4021 tgagcgccgt caattacgcc gtcggcgtca tcgaggcgaa gggcaaggcc gccttggacg
     4081 agctgaagaa ataccgtttc ggcgaaggca actacgtcta cgtcacggac atgggggcag
     4141 tggtgatcat gcaccctgtc gcgcccgagc tgctcaacaa ggactgcacg cagatcaagg
     4201 acgcaaaagg caagttcttc ggcgcggaga tgaagggcaa ggcggagaag tacggctcgg
     4261 gctggacgtc gtactggtgg cccaacccga agaagaacaa cgcaccagat ctgaaatgcg
     4321 ccttcttcaa gacagcgaac atggcgggca ccaaggtgat cgtctacgcc gccgcgttcg
     4381 gcatatcgga ggcgggctgc cagtaacggc acggcagaaa gagcggcaag cgggccatac
     4441 agaaacaacc gggcggcttt cagggaagcc gcccggttgt tcttgtttcc gtcgatggtt
     4501 ccgatccccg cccttgagac gccctcaggt cacttcgata cgagcgtgct gatctggctg
     4561 gagagccagt cctgttgcgc ctgcagcgtg ctcagcatgg tctccatggc cacgaacttg
     4621 ctctcgagcg ccttctgttt cctctcgaga cgctcctcca tgtcctcgat cttcctgtcg
     4681 agcttgtcca tctgcgactg gagggattcc tgcctggtca ccacgtaccc gtcgatgctg
     4741 tcggtcatgg cgtctatggc ctcttcaagc ctcgaggcga cgccggtggt ctcataggtg
     4801 aggccgtccg cgctgaacag gctcaccacg tcctcgaagt tcgtctcgag gtacccttcc
     4861 agcgtgtcgt cgtcgatgga cagcttgccg tacttgtcga tgttcactcc tatgaggctc
     4921 aggtgatcga gcgtggacga aagaccgtcc acaccggaga ggatggcgtt gcggatcgtc
     4981 ttcttcagct gggcgagcga ggaatccgca tagaggggtt tctcggtgtc gcccgcgggc
     5041 gtgttctggt cgtctatgta agacatgagg tcgttgtagg cgctcacgaa actctctatc
     5101 ttgtccttta cgcccgagtt gtcgcggctt accgtgagcg tgatcgtggc gctctcgtcc
     5161 gcacccttga ggttcagggt cacgcctgcg atgacgtcgc ttatcaggtt ggaactcctc
     5221 gtgatcgtga acccctcgac cacgatctcc gcgtcctggc cggagacggt ctcgttcatg
     5281 ccaaggcccg aggagaacac gcccgagccg tcgacgatgt cgatgccgga tgcgcccgtc
     5341 tccttggagg ccacggtgag ccggtattcg tcgtctgcga ccttgatgat cgaggcggtc
     5401 acacctgccg ggtcatcccc ggagttcagg gcgttgatct tgtcccggat gtcttcgagc
     5461 gtgtccgtcg cgtcgatcgt caggtccctg ccgttgagcg tgaccgtgcc ggagatcccg
     5521 agctccgtgt cgtcggaagc gaacgcggcg cttgcgaact tctgcgcctg ggccagcctg
     5581 ttgacggtga tcgagtacga gccttggctc gcgttggtcc ccaccgcgta gtcgaggatg
     5641 tcgctcaccg acttgctcga tccgcttatg gccgcctgcc cgctgtagac ctcgaagtcg
     5701 tcgctcgagg agagggcctg ggcggccgac atgagcgagt tgagcttcgt gttgacctct
     5761 cgccacgccg acttctgggc ctcgagcgcg gtcttcctct cttcgaggag cgtgatgggc
     5821 ctgcgctcga tggccatgag gtcagagatc acggactgcc agtcgagccc cgaagtgagc
     5881 cccgagatgt acgttgccat gggttccccc tcttcccccg aaagatcggc cccgcccccg
     5941 cgtccaacgg gagcgggcct tgtctctccc tacacggtcc gctccacgaa cagcgaagac
     6001 atctccctca ggcggcgcct gaggttcatg atctcctcgg gagggatctg cttgatcacc
     6061 tcaccgctgt ccttgtccac gatcctgatc accacgtcct tcgactcctc gtctatcctg
     6121 aactgcaggg actggttcaa gaccgcaagc tgctcgttga tcgcctcgat gtccttcttc
     6181 atctcatcgg cggaccgatc gtctttctgg aggggtttcg tcgccgcctg cgaatcgctg
     6241 tcgctgtccg ggatcctggg cttcgacacg gccactggct gaggaaccgg gccatcccgg
     6301 ctgagcctgt cgcccggcat cacggtcaag ctctcgatct tcatggccat acccgcctct
     6361 cacgctgcgg gtcacggggc gtcaccccgt gacccgcgcg ttctcagtca ttacccgaag
     6421 agactcagga cgacctggct cgacatgttg gcctgcgcga gcatggcggt gccggcctgg
     6481 agcaggatct ggctcttggt gaaggtcacc atctccgagg ccatgtccac gtccctgatg
     6541 acggactcgg cagccgagaa gttctcgatc tgcacctgga ggttggagta ggtgtagtcg
     6601 aggcggttca tggtcgcgcc gatgtcgccg aggacgcctg ccagggtgtt gatggcatcg
     6661 tcgatgagcc cgatggcgga gtcggcatgc gccgcggagt cgaccgccgc tgtcgagata
     6721 ccgagggtcg ttgtgtcaag gccgctgatg gcgatgttga tctgggagct cccgctcgag
     6781 tcggccgcgt tcatggcgcc gacctggaac gtgccgtcga agttgccgtc gaggagcttg
     6841 gtgccctggt aattcgtgtc gtccacgatg cggtcgatct catcgatgag cgactggaac
     6901 tctgcatcca gcttgtcgcg gtcggtgccc gcgttcgccg aggatgcctg gacggcgagt
     6961 tccttcatcc tctcgaggat gctctcgatc tggttggccg aaccctcggc gatctgcagc
     7021 agcgccttgc cctctgtcac gttgcgggat gcgaccgtga gcgacttgac gttggcgcgg
     7081 agcctgttgg cgatggcgag gcccgcggcg tcgtcggccg ccttgttgat gcggtagccg
     7141 ctggagagcc gctccatgct cttgttcatg ccggcttcgg tgacgaggag ctgacggtgt
     7201 gcgttgattg ccgctacgtt ggtgttgatg cgaagtgcca tgattcaatc ctccttgatt
     7261 tttgtttggt tccgaccatc cgtggccggg gtgttcattt gtgatcagtt tttcggtact
     7321 gatcagacct cctgtttcct cacctccttt ttaggttttt ttggtcatac agtctctctt
     7381 atcgtcactc cgtttgttaa ctttagcatt tttcttgcgc gcctctatat tctgtcctcg
     7441 ggtaggcacc gggaagcggc aaaacacccc tgctccatca gacgccaggt ccggaccttc
     7501 cttttgttgt tcattgttgt ttaccgggct cacgacaacg ttgcgcaggg ctgcccgtca
     7561 atttattgac aaataccccc cgcgacgatt tccatcccat cgatattatt atgcattttt
     7621 aagggcattg gcgggcatgt atcttgctaa aaattgcaga caaaggcagg cccatgatgc
     7681 acgacagctc tatatgtctt tatccatggg tggttgatga cgaatggcca cgagatcgtt
     7741 ccatgagccg ggcatggagc tctgcaaacc cacttgaggg acagggaagc tgatatggaa
     7801 agacctctcg tgactattgc ctgcacaact tacaaccacg agaagtacat cggtcagacc
     7861 atcgatgcct tcctggaaca gaggacgacc ttccccgtcg agatcatcat caatgatgac
     7921 gcctccacgg acaacacccc ccggatcata cgggagtatg ccgagaacca ccccggcctg
     7981 atcgttccca tctttcaggc ggagaaccag tactcgaaag gaaggaaacc cttcatcgag
     8041 ttcgtgttac cgcgggcacg gggaacctat atcgccctgt gcgagggtga cgactactgg
     8101 accgatcctg ccaagctcca gaagcaggtg gatttcctgg aagccaaccc cgactatgtc
     8161 ttgtgttacc acaacgccag gatagtcgac gaatacggtg cactggtgag tgcatccaag
     8221 ctgcctgatg acctgaagcg ggatctgtcc gccgaggaat tgatcagggg cgccatgatc
     8281 ctcactctga ccctttgctt ccgcaacatc gtcaaggaga tcccggaaga gctgttcaag
     8341 gtgtacaacg gggacaagtt cttcacctct ctgctgggca ggcacggcaa aggcaagtac
     8401 atggacgata tcacggacgc cgtgtaccgg aagcattcgg attccatctg gtcgagcttg
     8461 gacccggtcc gccagatcta ccacaatgga gtgacccggg cttggatgca ccgttattac
     8521 aagaggatcg gcgagacgca ccatgccgac tattttcgtg accaggtgat tatgcatttc
     8581 acgcgaacgc tccaggaaat agccgcaaga accgggaagg catatgatga tattgtagaa
     8641 gacatcttca gcaacttcac ggatatcgtc gaccaggcat cacaggagcg attgcggtca
     8701 ctcatgaccc ctgcgcgtca ggtaatccat tccaccgcgg caacagcttc atcggcagcc
     8761 catctcaccg ccggacaatc cggtaaaacc ggggcggccc gtttatccga cgtccctccg
     8821 gatggtgaat acatgagctg cacctacata gagaacgcca tcgccttcta ttacgacaac
     8881 ctcgtctaca catgctgtta tccgggctac atccaagacg agggaatcct tgcggacctt
     8941 tcccgcggcc cgttgaacac ggctgacatc attgcgagga agagagagat catccgaagg
     9001 aggatgaaca aagagttcgg cggctgtgca ggctgtccac ggttcgtgaa aaagaggtgg
     9061 cgccggaact gggatatctc catctcccac gtgaatctca atcatttgct tgcgtgcaac
     9121 ctgaaatgtc tccactgcgg atacgtggag aaggtcggga gacaccagcc gacttccaaa
     9181 gagtcgatca tggatgccgt ggccaccctg caagagggag ggttgttgga gccgggcgcc
     9241 gagttctaca tcgggaacgg tgagccctcc atcaatgccg acatggacga cctgatcgct
     9301 ttcctgctgg acagaaacta ttctgtcgtg gtccagtcca acggcacggt atacaaggac
     9361 atctatgccg ccggagtgaa tgccggaaag ctgaggctga tcctcacccc cgatgccggc
     9421 acacgggact cctacctgaa gatcaagggg agggattact tccaccgggt ctggaggaac
     9481 atcaggaagt atgcctcgga aacgtcaggc aacatcacgg tgaagctcat cctgcaggag
     9541 gggaacatca acgatgcgga gccgtttctg gatctctgcg attcggcaag ggtgagagac
     9601 atcgtggtgg atgtcgatct gcacatacgg gatcgcatca gcaaagagat tgcggctgca
     9661 gcaca
//
LOCUS       DAOSCR010000015         9639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4631_length_9639_cov_3.368635, whole genome shotgun sequence.
ACCESSION   DAOSCR010000015 DAOSCR010000000
VERSION     DAOSCR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4631_length_9639_cov_3.368635"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLT69_00965"
     CDS             <1..938
                     /locus_tag="PLT69_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13051.1"
                     /translation="GFDPKVGVILMAATNRPDILDPALLRPGRFDRHVLVDRPDKKGR
                     EEILRVHMKNVKTAPDVDPGRIASMTPGMVGADLANIVNEAALLAVRKGKREVGMEEF
                     EEAVERIVAGLEKKNRLINERERKIVAHHEMGHAIVAMSLPGADRVQKISIIPRGIAA
                     LGYTMQVPTDDRFLMSRAELLDKIATLLAGRAAEELVFSDVSTGAHNDLARATDIAKS
                     MVKEYGMSEKLGQVYLSSDRGPRLLDIGKVERTDYSEATAQAIDDEVRRIIQDQYRVA
                     RDILSSRMDVLEKGAQMLLEREKLDGEEVASLLGA"
     gene            1800..4061
                     /locus_tag="PLT69_00970"
     CDS             1800..4061
                     /locus_tag="PLT69_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13052.1"
                     /translation="MKDDLFIQCFNTSPIPGVITTAADGVIVEVNDAFVQMSGYRRED
                     LVGRRTLETGLWVDPADRARVLAELGAKGKVQNLEVRFKTASGSEHDCILNGVSFTYS
                     QVPCHLFMAVDITRRRRTQDALEKAEARYRALVENSLSGVAIFRAVKDGGDFEYVEFN
                     RAAERIDSIKREDVVGKTLLEVFPGSRASGLYQALLRVYRTGVGEYLPVYFYKDQRIS
                     GWRESFVYRLPSGELVEVYSDQTALIRAQRDLSERNRALTSLIGNLPGMVYRCRHDRD
                     WTMVAVSEGCLALTGYAVEDLVNNEKIAWGRVIVPKDRDRVWDEVNRALEKRENYELT
                     YRIRTAGGDVRWVWDHGCGVFSPEGRLLFLEGFAFDITEHRRLQDKLRMTEERFARIF
                     QASPDWVSITAMEDGAYRDVNEGYLKASGYTRDEIIGRTSLEIDIWERPSDRKRIVRK
                     IKEQGSVRNEEVRFRTKSGELKHVLWSAVPITLDDEECILGWGRDITEYKLLEEELMR
                     AYKMEAVGRLAGTIAHDFNNYLTAIDGFCELILLKADDKESVVRSVGKIREVKKSASS
                     LIKQLLSFSRKQPARPVPVDLNEVVTSMKDLLTPVMGQKVDLSMELSGEPCVVLADRS
                     QLEQTIMNLSLNARDAMPRGGRFTVKTAHETIDETSAGMHVDLEPGRYISLTVSDTGL
                     GMDEETASHIFDPFFTTKSMGKGSGLGLTIVYNIVRQWGGCITLQSEPGKGSTFTIYL
                     PAHAEAQRREARP"
     gene            complement(4259..6235)
                     /locus_tag="PLT69_00975"
     CDS             complement(4259..6235)
                     /locus_tag="PLT69_00975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ13053.1"
                     /translation="MVFCDNPKARGPMMHCGFADADARISQLKTLYPDRFPEPGAIFG
                     HIKRGDRIFVATGCAEPRHLSRSFLDHARANPKAFFDAELSHLVALGLTGEPLDDFRP
                     NFRQHFFFVAHSTRDAVTRGLADYTPSAMSQVSRLIKRGLMPVDVAFIQVSPPGSSGR
                     LSLGVSVDVMKEVIENSRLVIAQVNAFMPYTHGDSTIDPEQVDFFIHHDEPLVEFHYP
                     PPDDVTRAIGSYVARLVPDGSTIQAGYGRVVNAVLEGLGTKRHLGVHTEMLSSGIIDL
                     MRAGVVDNAKKSADALKTVASLCLGTSDMFAFMHDNPAIAFMPSSYTNDPSVIARQER
                     MVAINSALQIDLTGQASSESFGPGFYSGIGGDVDFMRAVSASPSGRTILAMPSTAKGG
                     AVSRIVARLGEGAGVTYNRSDIQYVVTEYGICHLQGKNIRERAMSLVAVAHPRFRPSL
                     VEEAKRLGLVPEDQPFFTGPSGLYPTDVETHRTTRTGLTVFLRPIKISDEELHKKFVY
                     SLSERTLTMRFLSPKTEFPRRERQRLVVIDYTRHMAILALLEGPETEEIVGIARYAVE
                     GDEHKAALAVVVKDDFHNQGIGTVLLDYVTFLARKQGLLGFTAEVFADNRAVMRMLRR
                     MREMGHDVKSSISDGVLSVTITFGHMDRPQRQES"
     gene            6332..7051
                     /locus_tag="PLT69_00980"
     CDS             6332..7051
                     /locus_tag="PLT69_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOJ13054.1"
                     /translation="MAVVEWKKDGTVAVLTMNTSENRHNPSYASGMLSCLDEIEADQD
                     IKSIVLASVDRKNWSLGIDLNWMMDAVNRGAGDEIRAFMYSLNTLFKKILTIPMPVIA
                     AINGHAFGDGAIIACTCDFRFMRSDRGFFCFPEVDINIPFLPGMLAMVKKAIPYYKVE
                     ELVFTGKRATGKELEECHAVVKACEGEEELMKEAMAFARTFAKGRVIFREHKWRLNRH
                     IVEIMDREDPAYIDPLNLMVS"
     gene            7077..8093
                     /locus_tag="PLT69_00985"
     CDS             7077..8093
                     /locus_tag="PLT69_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ13055.1"
                     /translation="MRGPGRESNGRFMPPYWLRGAYTQTVLASSRVRAVGAGDLEGSS
                     REITLTVTGGVRLVGSLSHSPGRKGLVILLHGWEGSARSTYILHAGRFFHRKGYSVFR
                     LNFRDHGDTHHLNEGLFRAVMLDEVFQAVSLAAGFSGEAPCYLMGFSLGGNFALRIAR
                     HCAQEPITHLRHVLAVSPVIDPSHATDAIDRSPLLRWYFVKKWRRSLRRKQELFPSLY
                     DFSAVLAERSVRAMTEALFSSLKAEYDTEGYFRSYAIHPETLEDVTVPTTIVTAQDDP
                     AIPVEDFSRLRLSPAVSLVIHDFGGHNGFLEGLFGPTWCEREALRLFDADSTLRPPPP
                     AGPA"
     gene            8371..9111
                     /locus_tag="PLT69_00990"
     CDS             8371..9111
                     /locus_tag="PLT69_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ13056.1"
                     /translation="MSEAKPIHDLLEFVSCSLGEAAGPEYRHEAALLQIELFENMVQN
                     CAVAIFAIDVRHRVIYWNKACEALTGHPAHRMLGTTDHWMPFYAERRPTLSDLIVDGE
                     TSRVDDLYTLHGPSLLVPEGLHAEGWFQDVGGRRRYLIFDASPVRLGSGELLGAVETL
                     QDITELKQMEEDKERLNKELVDAMNQIKTLRGLIPICAACKKIRDDKGYWTHLEHYLE
                     EHSDAVFSHGLCPDCFREYFPEASKKKD"
     gene            9299..>9639
                     /locus_tag="PLT69_00995"
     CDS             9299..>9639
                     /locus_tag="PLT69_00995"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ13057.1"
                     /translation="MLTSGQMERYAGVLMWGLATARGRRCKKGDIVLIRADERALPLA
                     ERVYELVVEMGCHPVMRVTLTPVMERAFYGKSSTSQLVFCTPGERELFSSLNGLVTIR
                     APESITHLADV"
BASE COUNT         1785 a         2950 c         3271 g         1633 t
ORIGIN      
        1 acgggttcga tcccaaggtc ggggtcatcc tcatggccgc cacgaacagg cctgacatcc
       61 tcgacccggc gctgctcagg cccgggaggt tcgacaggca cgtcctcgtg gacaggccgg
      121 acaagaaagg ccgggaggag atcctcaggg ttcacatgaa gaacgtcaag acggctccag
      181 atgtggatcc gggccgcatc gcatccatga ccccgggtat ggtgggtgcg gatctcgcga
      241 acatcgtgaa cgaggccgca ctgctcgcgg tacgcaaggg caagagggaa gtgggcatgg
      301 aggagttcga ggaggccgtc gagaggatcg tcgcaggcct tgagaagaag aacaggctca
      361 tcaacgagcg tgagcggaag atcgtcgccc accacgagat gggccacgcc atagtcgcca
      421 tgagccttcc cggcgcggac cgcgtccaga agatatccat catcccccgc ggcatagcgg
      481 cgctcggcta taccatgcag gtgccgaccg acgacaggtt tctcatgagc agggccgagc
      541 tcttggacaa gatcgcgacc ctgctcgccg ggagggcggc cgaggaactc gtgttctcgg
      601 atgtctccac cggggcgcac aacgaccttg cacgcgcaac ggacatcgcg aagagcatgg
      661 tcaaggaata cggcatgagc gaaaaactcg gccaggttta cctctcgagc gaccggggac
      721 ctcggctcct cgatatcggg aaggtcgagc gcacggacta cagcgaggcg accgcgcagg
      781 cgatcgacga cgaggtcagg cgcatcatcc aggaccagta ccgtgtcgca cgggacatcc
      841 tctcctcgag gatggacgtg ctcgaaaagg gcgcgcagat gctcctggag agggaaaagc
      901 tggacggcga ggaggtggcc tcgctcctcg gcgcgtaagc ggcgccttga gcgtcgctgc
      961 cggtctcgtg ccgcctccgg cggggagcgg gcagaatccg tggcaagaga caggcttctc
     1021 tcgaggcttc tctctcggga ccccatcctc gaactgtcca ggacccgctg ccgtcctgcg
     1081 tcctgccagg gggaggatgc ggaccgtatc gtcgaggagg ccctgcggcc ctccgcggag
     1141 gtgaccttgc ggttctccag gcgtcaatgg cccgtgcgac cgtttcccac cttgagccct
     1201 tcacggctgc gtgcacctgt ggggtgtcgc atgcgggcat ggcgggactt tgtccggaca
     1261 tgtcccgcga aaggaacagg ggcgcccaag caggcgatgg ttcccggtgt gcatgggcag
     1321 gcgtccaggt cgaatgaaag ggccgtctcc ccctcgtgac ggtggcggtc cccagggcct
     1381 ggatacccag aggtccgcaa gtcctggaca gggcatgacc cttgccgcgg ccctatccta
     1441 cgggtagggc ttggtccctc ccatgactgg ggcacggtgc ttacgtggac atacgggcag
     1501 gggacatgtt gaacgaggtt atcaagggga cattccggac gagtcttgac acgtgagccg
     1561 gcaaaccttg actctcggtg gctatgcatt atgaatgagc aggcagagga gcatgtccag
     1621 gaccgagccc tctgtttccc tgcgcccgag gggtccatgg catcgggtct tcagtcgggg
     1681 gcgccgaacg tgaccttcag gcctcgaggc ggcggcgaca gcgactgcag ggcgggaatg
     1741 gaccggtccg ccgggcggcc gtgaagcctc cacggtgagg ccggctggag ggtatgtgca
     1801 tgaaggacga cctgttcatc cagtgtttca acacgagccc cataccaggc gtcatcacga
     1861 cagcggctga cggggtgatc gtcgaggtca acgacgcctt cgtccagatg agcgggtacc
     1921 ggagggagga tctcgtcggg cgacgcacgc tcgagacggg cctgtgggtc gacccggccg
     1981 acagggcgcg ggtgctcgcc gagcttggtg ccaaagggaa ggtccagaat ctcgaggtta
     2041 ggttcaagac cgcctcgggg tccgagcacg actgcatcct caacggggtc tccttcacct
     2101 actctcaggt gccgtgtcac ctcttcatgg cggtggacat aacgagacgc aggaggactc
     2161 aggacgccct ggagaaggct gaggcgaggt accgcgccct ggtggagaac agcctgagcg
     2221 gcgtggcgat cttcagggcg gtcaaggacg ggggggactt cgagtacgtg gagttcaaca
     2281 gggcggccga gcggatcgac tcgataaaac gggaagacgt cgtcgggaag acgctcctgg
     2341 aggtctttcc cgggtcccgg gcctcggggc tgtaccaggc cctcctcagg gtctacagga
     2401 cgggggtcgg agagtacctg cccgtgtact tctacaaaga ccagaggata tcgggctggc
     2461 gcgagagctt cgtgtacagg ctgccgtcgg gcgagctcgt ggaggtctac tcggaccaga
     2521 cggccctcat cagggcccag cgggacctct ccgagcgcaa ccgggcgctc acctcgctca
     2581 tcggcaacct gccgggcatg gtgtacaggt gcaggcacga ccgtgactgg accatggtgg
     2641 ccgtgagcga gggctgcctc gctctcacgg ggtatgccgt cgaggatctc gtgaacaacg
     2701 agaagatcgc ctggggcagg gtgatcgtcc cgaaagaccg cgacagggtg tgggatgagg
     2761 tgaacagggc tctggagaag agggagaact acgagctgac ctaccgtatc aggacagccg
     2821 gcggtgacgt caggtgggtc tgggaccatg gctgcggggt cttctcgccc gagggcaggc
     2881 tcctcttcct cgaggggttc gccttcgaca tcacggaaca caggcggctc caggacaagc
     2941 tgcgcatgac cgaggaacgc ttcgcgagga tcttccaggc gagccccgac tgggtctcca
     3001 tcacggccat ggaggacggc gcgtaccggg acgtgaacga gggctatctc aaggcgtcgg
     3061 gttacacgag ggacgaaatc ataggccgca cctctctcga gattgacatc tgggaaaggc
     3121 cctccgaccg caagaggatc gtgaggaaga tcaaggagca ggggtctgtc cgcaacgagg
     3181 aggtacggtt caggaccaag agcggcgagc tcaagcacgt cctgtggtcg gcggtcccca
     3241 taaccctcga cgacgaggag tgcatcctcg ggtggggcag ggacatcacc gagtacaagc
     3301 tcctcgagga ggagctcatg cgggcctaca agatggaggc agtcggcagg ctcgccggga
     3361 ccatcgccca cgacttcaac aattacctca ccgccatcga tgggttctgc gagctcatcc
     3421 tgctcaaggc cgacgacaag gaatctgtcg tcaggagcgt ggggaagatc agggaggtca
     3481 agaaatcggc ctcctccctc atcaagcagc tcctgtcgtt cagcaggaag cagcctgcca
     3541 ggccggtgcc tgtggacctg aacgaggtcg tgacgagcat gaaggacctc ctcacgccgg
     3601 tcatgggcca gaaggtggat ctgagcatgg agctctccgg ggagccgtgc gtcgtgctcg
     3661 ccgacaggag ccagctggag cagacgatca tgaacctctc gctgaacgcg agggacgcga
     3721 tgccccgggg gggcaggttc acggtgaaga ccgcgcatga gaccatcgac gagacctctg
     3781 ccggcatgca cgtggacctc gagccgggaa ggtacataag cctcaccgtg tccgacacgg
     3841 gcctcggcat ggacgaggag accgcatcgc acatcttcga ccccttcttc accaccaagt
     3901 ccatgggcaa gggcagcgga ctcggcctga ccatcgtcta caacatcgtg aggcagtggg
     3961 gcgggtgcat aaccctccag tccgagccgg ggaaggggtc gaccttcacg atatacctgc
     4021 ccgcgcacgc cgaggcccag aggcgggagg cgaggccgtg acgagaaggg cctttagctt
     4081 cggcggcctg ccccggcggg aaaggcggcg ggtggagagg gggcctgccg ctcatgcggg
     4141 ccttcccggc acgtgaccgg acggcggccg ttggtgggct gtgccggccg ggtccgccgt
     4201 ggggttctct tcgccgggga aaagagccgg gcggcacggc tacgggtgtt caggcctgtc
     4261 aggactcctg cctctggggt ctgtccatgt ggccgaaggt gatcgtgacg gagaggacgc
     4321 cgtcgctgat cgagctcttc acgtcgtgtc ccatctcgcg catgcgcctg agcatgcgca
     4381 tcacggccct gttgtccgcg aagacctcgg cggtgaagcc gaggaggccc tgcttgcgtg
     4441 cgaggaaggt cacgtagtcg agcagcacgg tgccgatgcc ctggttgtga aagtcgtcct
     4501 tgaccaccac ggcgagagcg gccttgtgtt cgtcgccctc gacggcgtaa cgcgctatgc
     4561 cgacgatctc ctccgtctcg gggccttcca ggagggccag gatggccatg tgccgcgtgt
     4621 agtctatgac caccagtctc tgcctctccc tcctggggaa ctcagtcttc ggggagagga
     4681 agcgcatggt cagcgtcctc tcggagagcg agtatacgaa cttcttgtgc agctcctcgt
     4741 ccgatatctt gatgggcctc aggaacacgg tcaggccggt tctcgtggtg cggtgggtct
     4801 ccacgtcggt ggggtacaag ccgctggggc cggtgaagaa cggctgatcc tccggaacga
     4861 ggccgagccg tttcgcctcc tcgacgagag agggcctgaa cctggggtgc gcgaccgcga
     4921 cgagggacat ggcccgttcc ctgatgttct tcccctgaag gtggcagatg ccgtactccg
     4981 tgaccacgta ctgtatgtcc gaccggttgt acgtcacgcc cgcaccctcg cccaggcgcg
     5041 ccacgatgcg cgagacggcg ccccctttcg ccgtcgaggg catggcgagg atggtcctgc
     5101 cggaggggga tgcggacacg gccctcatga agtcgacgtc gccccctatg ccgctgtaga
     5161 aaccgggccc gaacgactcg gacgaagcct ggcccgtgag gtctatctgg agggcggagt
     5221 tgatcgccac catgcgctcc tgcctggcga tgaccgaggg gtcgttggtg taggaggagg
     5281 gcatgaaggc gatcgcgggg ttatcgtgca tgaaggcgaa catgtcggac gttcccaggc
     5341 acaaagacgc gaccgtcttg agcgcatcgg cgctcttctt ggcattgtcc accacgcccg
     5401 ccctcatgag gtcgatgatg ccgctcgaga gcatctcggt gtggactccc aggtgcctct
     5461 tcgtgcccag accctcgagc acggcgttga ccaccctgcc gtaacccgcc tggatggtgc
     5521 tcccgtccgg cacgagcctt gccacgtacg atcctatggc gcgggtgacg tcgtcgggcg
     5581 ggggatagtg aaactccacg aggggttcgt cgtggtggat gaagaagtcc acctgctccg
     5641 ggtcgatggt gctgtccccg tgggtgtagg gcatgaacgc gttcacctgg gcgatgacga
     5701 gccgcgagtt ctcgatgacc tccttcatga cgtccacgct cacccccagg ctcagcctgc
     5761 ccgacgagcc gggcggggac acctggatga acgcgacatc cacgggcatg agcccccgct
     5821 tgatgagcct cgacacctgg gacatggccg agggcgtgta gtcagccagc cccctcgtca
     5881 ccgcgtccct ggtgctgtga gccacgaaga agaaatgctg gcggaagttc ggcctgaagt
     5941 cgtccagggg ttcgccggtg agtcccaggg cgaccaggtg ggagagctcc gcgtcgaaga
     6001 atgccttcgg gttggcccgc gcatggtcca ggaaggacct cgagaggtgc ctcggctcgg
     6061 cgcaccctgt cgcgacgaag atcctgtccc ccctcttgat gtgcccgaag atggcccccg
     6121 gctcgggaaa cctgtccggg tagagcgtct tgagctggct gatcctcgca tcggcgtcgg
     6181 cgaaaccgca gtgcatcatc ggcccccttg ccttggggtt atcgcagaat accatcgacc
     6241 catcaagggt caaccgcgcg gggaacgaat gaatttccaa tgttgaccca aggtgtgcta
     6301 cagtgtccca tatcatcgag aggagggcgc catggcggta gtggagtgga agaaggacgg
     6361 gacggtcgcc gtccttacca tgaacaccag cgagaacaga cacaaccctt cgtacgcctc
     6421 cggcatgctt tcgtgcctgg acgagatcga ggccgaccag gacatcaagt ccatcgtgct
     6481 cgcgtccgtg gacaggaaga actggtccct ggggatcgac ctcaactgga tgatggacgc
     6541 ggtgaacagg ggggcggggg acgagatcag ggcgttcatg tacagcctga acaccctgtt
     6601 caagaagatc ctcaccatcc ccatgcccgt gatcgcggcg atcaacgggc acgcattcgg
     6661 tgacggcgcg atcatcgcgt gcacctgcga cttcaggttc atgcgctcgg acaggggctt
     6721 tttctgcttc cccgaggtgg acatcaacat cccgtttttg cccggcatgc tcgccatggt
     6781 gaagaaggcg atcccctact acaaggtcga ggaactcgtc ttcaccggca agagggccac
     6841 gggcaaggag ctcgaggagt gccacgcggt cgtgaaggcc tgcgaggggg aggaggagct
     6901 catgaaggag gccatggcct tcgcgcggac gttcgcaaag ggcagggtga tcttcaggga
     6961 gcacaagtgg cgtctcaaca ggcacatcgt ggagatcatg gaccgggaag accctgcgta
     7021 catagacccg ctcaacctca tggtgtcctg atcccgcgtg gagggggagg ggccgggtga
     7081 gaggtcctgg cagggaatcg aacggccggt tcatgccgcc gtactggctc aggggcgcct
     7141 acacccagac ggtcctcgcc tcgtcgaggg tcagggccgt gggtgccggc gacctggagg
     7201 gctcgtccag ggaaatcacc ctcaccgtga caggcggcgt ccgcctggtg ggctccctct
     7261 cccattctcc gggaaggaag ggcctcgtga tcctcctcca cggatgggag gggagcgccc
     7321 gttcgaccta catcctccac gcggggaggt tcttccaccg gaaggggtac tccgtgttca
     7381 ggctcaactt cagggaccac ggggacaccc accacctcaa cgagggcctg ttcagggccg
     7441 tcatgctcga cgaggtgttc caggccgtat cccttgcagc ggggttctcg ggagaggccc
     7501 cctgctatct catgggtttt tccctgggcg gcaacttcgc gctcaggatc gcgaggcact
     7561 gcgcgcagga gccgataacc catctcaggc acgtgctcgc cgtgagcccg gtcatcgacc
     7621 cttcgcacgc caccgacgcc atagaccgaa gcccgctgct caggtggtac ttcgtcaaga
     7681 agtggaggag gtccttgcgg aggaagcagg aactcttccc ctcgctctac gacttctcgg
     7741 ccgtcctcgc ggagaggtcc gtcagggcca tgacggaagc actcttttcc tccctgaagg
     7801 cggaatacga caccgaaggc tacttcaggt cctacgcgat acaccccgag acgctcgagg
     7861 acgtgacggt ccccaccacg atcgtcacgg cgcaagacga cccggcgatc ccggtcgagg
     7921 atttctcccg cctcaggctc agcccggccg tcagtctcgt gatccacgac ttcggcggac
     7981 acaacgggtt cctcgagggt ctcttcggcc ccacctggtg cgagagggag gccctccggc
     8041 tcttcgatgc ggactccacg ctcaggccgc ctccgcctgc cgggcccgcc tgaggttcca
     8101 ctgcctgcag tggttcgcgc cttgcgaggg tcgtgcactc cgggtgcggc tgccaggcgg
     8161 aaccggcgcg tcgtgacctg gtcatggtat gaggcgcgcc tttgaagcgg gcatgtgcgg
     8221 catccgagcg cggcccggca tggtgtgagg ctctcctggg aacctcgcgc aacgaggcct
     8281 tcttctgcgt gcgaccctgc gtgtgggcca agcggggaat ccttgaaaag gcgtccggcc
     8341 gccattatta ggtgggtacg ggagcgagcg gtgagcgagg caaaacccat ccatgacctt
     8401 ctcgagttcg tctcgtgcag cctgggcgag gcggcgggcc ccgagtaccg gcacgaggcc
     8461 gccctcctgc agatcgagct gttcgagaac atggtgcaga actgcgcggt ggcgatcttc
     8521 gctatcgacg tgaggcaccg ggtgatctac tggaacaagg cctgcgaggc gctcaccggt
     8581 cacccggccc accgcatgct ggggacgacc gatcactgga tgcctttcta cgcagagagg
     8641 aggccgacgt tgagcgacct catcgtcgac ggcgaaacat cccgggtcga cgacctctac
     8701 accctgcacg gtccgtccct cctcgtgccc gaaggcctcc acgcggaggg ttggttccag
     8761 gacgtggggg gccgaaggcg ctatctcatc ttcgacgcct cgccggtccg cctggggagc
     8821 ggggaactct tgggcgcggt ggagacgctc caggacatca ccgagctcaa gcagatggag
     8881 gaggacaagg agaggctcaa caaagaactc gtcgatgcca tgaaccagat caagacgttg
     8941 aggggcctca tccccatctg cgccgcctgc aagaagatca gggacgacaa ggggtactgg
     9001 acccatctcg agcactacct cgaggaacac tccgacgccg tgttcagcca cgggctgtgc
     9061 cccgactgct tcagggaata cttcccggag gcgtccaaga agaaggattg agcggcatgc
     9121 cccgcccgag gccttctcgg gccgcgcccg cctgcgggtc attcccctgg ccgcctgttg
     9181 cccggcccgc acgcaggaag cccttcgccg taagacgcca tgccgcaagg tgagagcggg
     9241 cttgtgcgaa ggcggttttg ctgttagatt cgcgtgtcag tattcaagag gggtgatcat
     9301 gctcacgagc ggacagatgg agaggtatgc cggggtgctc atgtgggggc tggcaacggc
     9361 caggggtaga cgctgcaaga agggcgatat cgtgctcatc agggcggacg aaagggccct
     9421 gccgctcgcc gagcgggtat acgagctcgt cgtggagatg gggtgccacc cggtcatgag
     9481 ggtcacgctg acccctgtca tggagcgcgc cttctacggg aagtcctcga cctcccagct
     9541 cgtgttctgc acaccgggcg agagggagct gttctcgtcg ctcaacgggc tcgtcaccat
     9601 ccgtgcgccc gaatccatca cccacctggc ggacgtgag
//
LOCUS       DAOSCR010000016         9334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4838_length_9334_cov_3.416424, whole genome shotgun sequence.
ACCESSION   DAOSCR010000016 DAOSCR010000000
VERSION     DAOSCR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_9334_cov_3.416424"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1307)
                     /locus_tag="PLT69_01000"
     CDS             complement(234..1307)
                     /locus_tag="PLT69_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13058.1"
                     /translation="MPDRDLLDEEIIDLTDLLEEGTPSGKPPEEAARKRPVTEPDSFD
                     LGKELATDLDIPIEEIDQDTAGILAEDGRATKAGPDMGIPLEETPATLQMRPEREPAL
                     STETELALKEAAEELGLAGEEELGLKPVSDVETLLGEPPAQGRAHGEPKPALSTEIDL
                     ALKEAAEELGLAGEEELGLKPVSDVETLLGEPPAQGRAHGEPKPSLSTEIDLALRETL
                     EEVPLTEKEQEVLLSETPEEGAEVTPAKALGGLAEPTPPSDREAVPGPGEAEAGPVPP
                     ALAGADRREPCLAEGGVPVGDVMGAVVEDFRKDMPALLESIVRPLVQELVQEIVASTR
                     EVLPGLVEKVIREEIEKLKKLAL"
     gene            complement(1351..2673)
                     /locus_tag="PLT69_01005"
     CDS             complement(1351..2673)
                     /locus_tag="PLT69_01005"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOJ13059.1"
                     /translation="MNQEAACRSIETTIKSLGRATIDSPVSGSTIFVEDHERVVIDTS
                     LGFFKRCMETGSEPVSFELAGPRRKIYFDPSKTKCAIVTCGGLCPGINDVIRSLVLEL
                     HYAYRVENIYGVPYGLQGFIPSYGHALVELTPDAVEHIHELGGTVLGSSRGSQDIGEI
                     VDALERLNMNIVFFIGGDGTLKAASSVAREALERGYRCAVIAIPKTIDNDIAFVSKTF
                     GFDTAVEEARKVITCAHAEAKGAPNGIGLVKLMGRDSGFIAANAALASRDVNFVLVPE
                     EDFDLEGPRGFLCALEKRIRTRGHAVVVVAEGAGQKFFTDMPEEKDASGNVRKHDIGV
                     YLKERIERYFQEVALPVNLKYIDPSYTIRSVTANASDSIFCGFLAQMAVHAGMCGKTD
                     MLIGLWNNVYVHIPTEICMQGRKKIDVGGPLWMSVREATGQPVMVNEG"
     gene            2789..3166
                     /locus_tag="PLT69_01010"
     CDS             2789..3166
                     /locus_tag="PLT69_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13060.1"
                     /translation="MPARVRSIFCARPRYAIGLLVGRHRDGLDKTEKELLEEMAKALG
                     NTGHQLEEVIEEMKALEEVMERTEDVEEYNLLVERFNDLHRTAMARREMLMIHREAIK
                     IYKHTVIDAVYPIPERKKKRTAP"
     gene            3163..3891
                     /locus_tag="PLT69_01015"
     CDS             3163..3891
                     /locus_tag="PLT69_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ13061.1"
                     /translation="MSADTRHDLIVIGGGPAGYTGALLGAKKGLKVCIVEQDRWGGTC
                     TNKGCIPTKAYMESLRVKGLVEKASRYGIEIEGAFQVKASLEAMRKRTERIVKRLAKG
                     VEFLLAKAGVTLIGSTARLEGARRVAAGGRVMEASAVLIATGARPRMPASFTLPGFIT
                     SDEVFDLERAPTSLVIVGAGAVGMEMAHIFSRLGTEVVVLEEQDRVLPLEDEEVSRLL
                     ASRYREVRFVTGARILPPGPVTAA"
     gene            3888..4535
                     /locus_tag="PLT69_01020"
     CDS             3888..4535
                     /locus_tag="PLT69_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ13062.1"
                     /translation="MTLDVETASGRTTEKAELCLVCTGREPVVPEGVQGSGVEVGEGG
                     GIHVDGHMRTSVPGVYAAGDVTGSHMYAYVASREAEVAVMGIVGEETAAMRYDLIPRV
                     VFTDPEVASVGKGVAGDREPGRQGVFPVSALGRARTMDESEGFARVACAPGGEISSVS
                     IAAPHATELIAWATLALNQGLTLEDFLEPICPHPTLSEILREAALDAAGLCIHKP"
     gene            4616..4996
                     /gene="hisI"
                     /locus_tag="PLT69_01025"
     CDS             4616..4996
                     /gene="hisI"
                     /locus_tag="PLT69_01025"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOJ13063.1"
                     /translation="MGKLLDFGKAGGLVPAIAQDAATGEVLMLAYMNEESFEETLRTG
                     RACYFSRSRNRLWRKGEESGNVQEVVEVLVDCDQDAVLLRVNQVGGAACHTGYRSCFH
                     RAVRDGALATTGERVFNPEEVYKK"
     gene            4993..5871
                     /gene="hisG"
                     /locus_tag="PLT69_01030"
     CDS             4993..5871
                     /gene="hisG"
                     /locus_tag="PLT69_01030"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOJ13064.1"
                     /translation="MSLIRLALPKGSLQTSTIELFRDAGWNISTSSRSYFPSIDDKDI
                     ECALVRAQEVSRYVESGLFDLALTGKDWILENGSDVVEVADLVYSKATYTPARWVLAV
                     DEKSPVRSIEDLRGKKIATELVNFTRRFFAERGIEVEVEFSWGATEAKVVEGLADAIV
                     EVTETGSTIRAHNLRIVDTLLVTNTKLIANRQSWANEAKRAKIEQIALMLRSALQAVG
                     MVGLKMNAPREKLEEVIAMLPALTSPTVSPLHDPGWFSLEVIVNESHSRSLIPELLKA
                     GARGIIEYPLKKVLDG"
     gene            5868..6614
                     /gene="map"
                     /locus_tag="PLT69_01035"
     CDS             5868..6614
                     /gene="map"
                     /locus_tag="PLT69_01035"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOJ13065.1"
                     /translation="MIVLKSPREIATMREANRILALLFEHLQGLIQPGVTTAELDQEA
                     ELFIRSHNAVPAFKGYRGYPATLCTSVNEEVIHGIPSKRKLRKGDIVSIDVGAVYEGF
                     YSDSARTFPVGEVSDDALRLMEATERALMEGIERARPGNRLHDISAAIQDVVERAGFS
                     VVREFVGHGIGRSLHEDPQVPNFGRKGSGPLLQEGMTLAIEPMVNQGTWKVEILDDGW
                     TAVTADGSLSAHFEHSIAITSNGPVILSVL"
     gene            complement(7227..8585)
                     /locus_tag="PLT69_01040"
     CDS             complement(7227..8585)
                     /locus_tag="PLT69_01040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ13066.1"
                     /translation="MKIKRVLPEIILLVSDVVVSWGAVHLAKYLQFLLPEVCLPDELL
                     GLKAGVYSAVTVLIFYFQDLYDWRFFKRISELASSILLAEGLCLIILAFLFFVIPSVG
                     IERDVLFMAIGITIVGTSAVRYALTRVRGSRYAGIRVVLFGDGENAKFLFREMRSSGY
                     PVLFEGYVGKVNWDLPLKRLGPAEEVRDIVERHDPDMLVVAPDGWRGTLPVEDLLKIK
                     LSMCEVVDAPTFYERVTGRILVEEIRPSSIIFTEGYQSSRLQDIVKRAFDLVFAVVGL
                     VLCAPVMLLTALAIRLDSPGPVFYFQNRVGKDGRDFKVIKFRSMYKDAEKDGPKWASE
                     HDPRITRVGRIIRKLRIDELPQFINVIKNDMSFVGPRPERRFFVDQLEKAIPFYALRL
                     HAKPGITGWAQINYPYGDTFDDAKEKLKYELYYMKHRSLWLDLVIIFQTIKIALKGRG
                     AQ"
BASE COUNT         1722 a         3024 c         2996 g         1592 t
ORIGIN      
        1 atgacggcgt gggccggaga accattggac agcacccccg aggccccctc ggaggaagag
       61 cctccccttc cccgtctatg acccgggagg aaggggcctg gcatgctgca ggcatgcggg
      121 agacaggcgg cgtgacctcc ctgtgggtgc gaggcctcca tgccctggct gcccgagacg
      181 acgggcaccg tcttccgcgg gaaagcagcg ccgcttcctg gaaacgggcg ccttcacagg
      241 gcgagcttct tcagcttctc gatctcctcg cggatgacct tctcgacgag acccgggagc
      301 acctccctgg tcgaggccac gatctcctgc acgagttcct ggacgagcgg cctcacgatg
      361 ctctccaaga gggcgggcat gtccttcctg aagtcctcga ccacggcgcc catgacgtcc
      421 ccgaccggca cgccgccttc tgcaaggcaa ggctcccgac ggtcggcacc tgccagggca
      481 ggggggacgg gaccggcctc agcctcccca gggccgggca cagcctcacg gtctgaggga
      541 ggggtcggct cggcgaggcc gcctaaggct tttgcgggtg tcacttcggc gccctcttcc
      601 ggcgtctcgc tcaacagcac ctcctgttcc ttctccgtca aggggacctc ctcgagggtt
      661 tctctcaagg ccaggtcgat ctccgtggac aaggagggct tgggctcccc atgcgcccgt
      721 ccctgggcgg ggggttcccc gagcagcgtc tccacgtccg agacgggctt gagcccgagc
      781 tcctcttccc cggccaggcc cagctcctcc gccgcctcct tcagggccag gtcgatctcc
      841 gtggacaagg caggcttggg ctccccatgc gcccgtccct gggcgggggg ttccccgagc
      901 agcgtctcca cgtccgagac gggcttgagc ccgagctcct cttccccggc cagacccagc
      961 tcctccgccg cctccttcag ggccagctcg gtctccgtgg acaaggcggg ctcccgctcc
     1021 ggccgcatct gcaaggtggc gggggtttct tccagcggta tccccatgtc ggggcccgcc
     1081 ttggttgccc ggccgtcctc cgccaggatc cccgccgtgt cctggtctat ctcttcgatc
     1141 ggtatatcga gatcggttgc gagctccttg cccagatcga acgagtccgg ttcggtcacc
     1201 ggcctcttcc tcgccgcctc ttccgggggt ttccccgagg gggtaccctc ctcgaggagg
     1261 tcggtgaggt ctatgatttc ctcgtccaag agatccctgt ccggcatggc aatctccttt
     1321 ttcgaccggc tccgcaacaa ccggaacccc tcagccctcg ttcaccatga cgggctgtcc
     1381 cgtggcctct ctcacgctca tccacaacgg cccgccgacg tctatcttct tcctgccctg
     1441 catgcagatc tcggtgggta tatgtacata tacattattc cacaggccga tcaacatgtc
     1501 cgttttgccg cacatgccgg catgtaccgc catctgtgca aggaaaccgc agaagatcga
     1561 gtcggaggcg ttggcggtca cgctccttat ggtgtagctc gggtctatgt acttcaggtt
     1621 gaccggcaga gcgacctctt ggaagtacct ctcgatcctt tctttcaggt agacgccgat
     1681 gtcgtgcttc ctcacgtttc ccgaggcgtc tttttcctcg ggcatgtccg tgaagaactt
     1741 ctggcctgca ccctcggcaa cgaccaccac ggcatggccc cttgtccgta tcctcttttc
     1801 gagggcgcag aggaaccccc gcgggccttc gaggtcgaag tcctcttccg ggacgaggac
     1861 gaagttcacg tccctcgatg cgagggccgc gtttgccgct atgaatcccg agtccctccc
     1921 catgagcttg acgaggccga taccgttggg agcacccttc gcctccgcgt gggcgcacgt
     1981 gatgacctta cgcgcctcct cgacggcggt atcgaagccg aatgtcttgg atacaaaggc
     2041 gatgtcgttg tcgatggtct tcgggatggc gatgaccgcg cacctgtacc ccctctcgag
     2101 ggcctcccgg gcgaccgatg atgcggcctt cagcgtcccg tcacccccga tgaagaacac
     2161 gatgttcatg ttgagccgtt cgagcgcgtc cacgatctcg ccgatgtcct gggagcccct
     2221 ggaagacccc aggaccgtcc ccccgagctc gtggatgtgc tcgaccgcat ccggggtgag
     2281 ctccacgagc gcgtggccgt acgacggaat gaaaccctgg agcccgtacg gcacaccgta
     2341 gatgttctcc acgcggtagg cgtagtgcag ctcgagcacg aggctccgta tgacgtcgtt
     2401 gatgccgggg cacaaccctc cgcaggtgac gatggcgcac ttggtcttcg aggggtcgaa
     2461 gtagatcttc cggcgcggac ccgcgagctc gaaggacaca ggctcggagc cggtctccat
     2521 gcaccgcttg aagaacccca gggaggtgtc gatgaccacc cgctcgtgat cctccacgaa
     2581 gatggtgctc ccggagacgg gagaatcgat ggtcgccctt cccaggctct tgatcgtcgt
     2641 ctcgatcgaa cggcaggcgg cctcctggtt catggtcgtc gtctcctttc ctagccggcc
     2701 tatcgacttt ccccgcggtt ttcttgaaga cggccgtcag gttccattgt accacgcatg
     2761 gtatatcgtg aacacagggg ttcatgtcat gcctgcacgg gtccgaagca tcttttgcgc
     2821 gaggccccgc tatgctatag gcttgctcgt gggcaggcac agggacggac tggacaagac
     2881 cgagaaagag ctcctcgagg agatggcaaa ggccctcggc aataccgggc accagctcga
     2941 ggaggtcatc gaggagatga aggccctcga ggaggtcatg gaacggaccg aggacgtcga
     3001 ggagtacaac ctgctggttg agaggttcaa cgaccttcac aggacggcta tggcgaggag
     3061 agagatgctc atgatccacc gcgaggccat aaagatctac aagcacacgg tcatagacgc
     3121 cgtctacccc atccccgagc ggaagaagaa gaggaccgcg ccgtgagcgc cgacacccgg
     3181 cacgacctca tcgtcatcgg cggcgggccc gccggataca ccggggcgct cctcggcgcg
     3241 aagaaggggc tcaaggtgtg catcgtggaa caggaccgct ggggaggtac atgcaccaac
     3301 aagggttgca tccccacgaa ggcgtacatg gaatccctga gggtcaaggg cctcgtagag
     3361 aaggcatcgc gctatggcat cgagatcgaa ggcgctttcc aggtgaaggc atcgctcgag
     3421 gccatgagga agaggaccga gcgcatcgtc aagaggcttg ccaagggcgt ggagttcctc
     3481 ctggccaagg ccggcgtcac cctgatcggc tccaccgcac gcctcgaggg cgcacgcagg
     3541 gtggcggcgg gggggcgggt catggaggcc tccgccgtcc tcatcgccac gggggccagg
     3601 cccaggatgc ccgcatcgtt caccctgccg ggattcatca cgagcgacga ggtcttcgat
     3661 ctggagcggg cccccacctc cctcgtcatc gtgggagcag gtgcggtggg catggaaatg
     3721 gcgcatatct tctcccggct cgggacggag gtggtcgtcc tggaggagca ggaccgggtc
     3781 ctcccccttg aggacgagga ggtctcgagg ctcctcgcct ctcgatacag ggaggtccgg
     3841 ttcgtcaccg gggcgcgcat ccttccgccg gggccggtga cggccgcgtg acgctcgatg
     3901 tcgagaccgc gtcaggaagg acgaccgaga aggcggagct ctgcctggtg tgcaccggga
     3961 gagagccggt cgtgcccgag ggggtccagg gctcgggcgt ggaggtcggc gagggtgggg
     4021 gcatacacgt cgacgggcac atgcgcacga gcgtcccggg cgtctacgcg gcaggcgacg
     4081 tgacggggtc gcacatgtac gcgtacgtgg catcccggga ggctgaggtc gcggtcatgg
     4141 gcatcgtggg cgaagagacg gcggccatga ggtacgacct catcccccgc gtcgtgttca
     4201 ccgacccgga ggtcgcctct gtcggcaaag gtgtggcggg cgacagggaa cccggcaggc
     4261 agggggtctt cccggtctcg gccctcggga gggcgaggac catggacgag tccgaggggt
     4321 tcgcacgggt cgcctgcgct ccaggagggg agatctcctc ggtcagcata gccgcgccgc
     4381 atgccacgga gctcatagcc tgggcgaccc tcgcgttgaa ccaggggctc accctcgaag
     4441 acttcctgga gccgatctgc ccgcacccga cgctgtccga gatcctgagg gaggcggcct
     4501 tggacgcggc cggcttgtgc atccacaagc cctgatctca aagatacggg cataaccccg
     4561 cacgcttgac accgctcgag caaacctgtt acagctctgc tcgcaggagg cgtccatggg
     4621 aaagcttttg gatttcggga aggcgggcgg gctcgtgcct gcgatcgcgc aggatgccgc
     4681 cacgggagag gtgctcatgc tcgcctacat gaacgaggag tctttcgaag agaccctgcg
     4741 caccggcagg gcatgctact tcagccgctc gaggaacagg ctgtggcgca agggcgagga
     4801 gtccgggaac gtccaggagg tcgtcgaggt cctcgtggac tgcgaccagg acgccgtgct
     4861 cctcagggtc aaccaggtgg ggggcgccgc ctgtcatacc gggtaccgct cgtgcttcca
     4921 tcgtgccgtg agggacggcg cccttgcgac cacaggggag cgcgtcttca accccgaaga
     4981 ggtctacaag aaatgagcct gattcgactc gcactgccga agggcagtct ccagacctcc
     5041 accatcgaac tcttcaggga cgccggctgg aacatcagca cctcgtccag gagctacttc
     5101 ccctccatcg acgacaagga tatcgagtgc gccttggtca gggcgcaaga ggtgtccagg
     5161 tacgttgagt cggggctgtt cgacctcgcg ctcaccggca aagactggat cctcgaaaac
     5221 ggctccgacg tcgtggaggt cgcggacctc gtctactcga aggccaccta cacgccggcg
     5281 aggtgggtgc ttgcagtgga cgagaagtca cccgttcgga gcatcgaaga tctcaggggg
     5341 aagaagatcg caaccgagct cgtcaacttc acgaggcgct tcttcgccga gagggggatc
     5401 gaggtcgagg tggagttctc ctggggtgca accgaggcca aggtcgtcga gggtctggcg
     5461 gacgccatcg tggaggtcac cgagaccggt tcgaccatac gggcgcacaa cctgcgaatc
     5521 gtggacaccc tcctcgtcac gaacaccaag ctcatcgcga acaggcaatc gtgggcgaac
     5581 gaggcgaaaa gggccaagat agagcagata gcgctcatgt tgaggtccgc cctccaggcc
     5641 gtggggatgg tggggctcaa gatgaacgcg cccagggaga agctcgagga agtgatcgcc
     5701 atgctccccg cgctcacatc cccgacggtc tcccccctgc acgaccccgg ctggttctcc
     5761 ctcgaggtga tcgtgaacga gtctcactcg cgctccctga tcccggagct cctgaaggcg
     5821 ggcgccagag gcatcatcga atatcccctc aagaaagtcc tcgatggatg atcgtcctca
     5881 agtcgcccag agagatcgcc acgatgcggg aagcgaaccg tatcctggcg ctcttgttcg
     5941 aacacctcca gggcctgatc cagcccggcg tgaccaccgc cgagcttgac caggaggccg
     6001 aactcttcat ccgctcgcac aacgccgtgc ccgcgttcaa gggctaccgg gggtaccctg
     6061 cgacgctctg cacctccgtg aacgaagagg tcattcacgg catcccgtcg aagaggaagc
     6121 tcaggaaggg ggacatcgtg agcatagacg tgggtgccgt ctacgagggc ttctactcgg
     6181 actcggccag gaccttcccg gtgggcgagg tgtccgatga cgccctgagg ctcatggagg
     6241 cgaccgagcg cgcgctcatg gaggggatcg agcgggccag gcccggaaac cgcctccacg
     6301 acatctcggc cgccatccag gacgtggttg agagggccgg gttcagcgtg gtcagggagt
     6361 tcgtgggcca cggcatcggc aggtccctgc acgaggaccc ccaggtcccc aacttcggca
     6421 ggaagggttc gggcccgctg ctgcaggaag ggatgaccct cgcgatagag cccatggtga
     6481 accagggcac gtggaaggtc gagatcctcg acgacgggtg gaccgcggtg acggccgacg
     6541 gctccctgtc cgcccacttc gagcacagca tcgcgataac ctcgaacggc ccggtcatcc
     6601 tgagcgtctt gtagaggcgc aagccccctg ccccatcacg ccccggacag ctcacccgcc
     6661 tggccgcacc gctcccatac cctttcatgc cgggcatcca ggaagaggcg gcatgccatg
     6721 actcaaccac caggacgcgt cgacccggaa cgaacctgtc ataccgggtt tccaggaagg
     6781 gtcatcctcc cacgacctcg acccggtcgg atgcccgcgt tcgacaggtg ccgcctaacg
     6841 gccctcaagg ggccgaacac gagaaaccat agacctgata cagctcaaag accacgtgaa
     6901 tgcgggacca cgacgaggtg aaccgaggca ctgcccctgg acgcgtcgct cacccgatgg
     6961 ggaccgggaa ggaaagggcc ctgtggcgga gaggaaaagg cgggcccgtg gggaaccggc
     7021 catcgacgtg cacggccgag acgggtatcg ggcgtcccct gcctccctgc acggtgccgc
     7081 gggaagagac gcaggtccgg gcttcaggcc gttttgagcg tgtccacatc gttgaagttg
     7141 cgctggggtc cgtgatggaa gagacgcagg ccaagtttca ggccgttacg agacctaccg
     7201 ggaacgcgca aggccgcggg gacggctcac tgggcgcccc tgcccttgag cgcgatcttg
     7261 atcgtctgga agatgatcac cagatccagc cacaaggacc tgtgcttcat gtagtagagc
     7321 tcgtacttga gcttctcctt ggcgtcatcg aaggtgtccc cgtaggggta gttgatctgt
     7381 gcccagccgg ttatcccggg cttcgcatgg agcctgaggg cgtagaaggg gatcgccttc
     7441 tcgagctgat ccacgaagaa gcgcctctcg ggcctcggcc ccacgaagct catgtcgttc
     7501 ttgatcacgt tgatgaactg cgggagttcg tcgatcctca gcttcctgat gatcctgccc
     7561 acgcgcgtta tccgggggtc atgctcgctc gcccacttcg ggccgtcctt ctccgcgtcc
     7621 ttgtacatgg acctgaactt tatcaccttg aaatccctgc cgtccttccc cacgcggttc
     7681 tggaagtaga agaccgggcc gggcgagtcc agccggatgg caagggcggt cagcagcatg
     7741 accggcgcgc acaacacgag cccgacaacg gcgaagacca ggtcgaaggc gcgcttgacg
     7801 atatcctgga ggcgtgaact ctggtatccc tcggtgaaga tgatcgaact ggggcggatc
     7861 tcctccacga ggatcctgcc ggtcacgcgc tcgtagaagg tgggcgcgtc gacgacctcg
     7921 cacatggaca acttgatctt gagcaggtcc tcaacgggga gcgtccccct ccacccgtca
     7981 ggggcgacga cgagcatgtc ggggtcgtgc cgctcgacga tatccctgac ctcttcggcg
     8041 ggcccgagcc tcttgagggg caggtcccag ttgaccttcc cgacgtaccc ctcgaagagg
     8101 acagggtagc cggaagaacg catctccctg aacaggaact tcgcgttctc gccgtcgccg
     8161 aacaggacga ccctgatgcc tgcgtatcgg gagccccgca cacgagtgag cgcgtatcgc
     8221 acggccgagg tccccacgat ggtgatcccg atggccatga agagcacgtc cctctcgatc
     8281 ccgacagagg gtatgacgaa gaacaaaaac gcgaggatga tgaggcagag gccctcggcc
     8341 agcaggatcg aagacgccag ttcggagatc ctcttgaaga acctccagtc gtagaggtcc
     8401 tggaagtaga agatcaacac ggtcacggcc gagtagacgc cggccttgag ccccagcagc
     8461 tcgtcgggca ggcacacctc gggcaggagg aactggaggt acttcgcgag gtggaccgcc
     8521 ccccatgaga ccaccacgtc ggacaccagc aggatgatct ccggaagaac cctctttatc
     8581 ttcatgcacc cttctctcgc ctcgtcccca tccgtgcacg aacctatgac cgggacgggc
     8641 gcctcggctc gaaagcggtc accacgcagg tcccgacccc gtccaccatc cgtctcgacc
     8701 ggcacgaacc ggggagacgc ccggccgggc tccttgtgta cgccgcccgt cgaacaagct
     8761 gaccatgagc atataccgta tcaataccac acagataccg tgatcggaag gtcatgatca
     8821 cagagggtct cttgcgtggt ggccccggcg ggttcccggc ctcgaaaggc cggccgcgga
     8881 ggccggtgag cctttcatgg gaaagggccc gacccagctc ggatcactga cccaagcgga
     8941 tgggccccct gaaaggcatc gttccgtcaa gaccctcagc ggtgacatcg atcgtccatc
     9001 acgaaagccc cttccagaga gacaccctca cggggcgtgc ccacgttacg agagaaaacc
     9061 ccgtctcttt gccggtgggc atcgcggaca caccgagacc atgcaagata cggtcgtgca
     9121 tcgccgccgc cgagtcttac ggatcgatac cccttaccgg ggagccgccc tgcggcggca
     9181 cacgaggata cgccgtgaga agccgtggcc gcctcccagg tcgcctcagg cgagcgcccg
     9241 acggggcggt gtccggggag ggaggccgac accgtgatct cgtatgcacg aggggaagag
     9301 tcggactggg gtacaggact cagccggaga ggtg
//
LOCUS       DAOSCR010000017         9298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4869_length_9298_cov_4.084064, whole genome shotgun sequence.
ACCESSION   DAOSCR010000017 DAOSCR010000000
VERSION     DAOSCR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_9298_cov_4.084064"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..965)
                     /locus_tag="PLT69_01045"
     CDS             complement(147..965)
                     /locus_tag="PLT69_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13067.1"
                     /translation="MSGLARILCVDDEPNVLSAIRRVFIDEDYEVLTATSVEDGLRIL
                     SEVQPVQVVIADYRMPGINGVDFLKEVCRRWPDSVRIVLSGYADTAAVVDAVNEAGIY
                     KFMAKPWNDRDLKHTVHNAVEHYRLATENARLNRDLQRANDELKVLNENLEAMVEQQT
                     QELLVQNRILMRAQEILDSLPLAVVGVDGDGIVVQANRAAREILGVGGRGLIGMDRRD
                     ALPEVINSFCGLVIEKGSARCRGELYGSPVTLMGNELGSPSKREGVVIAAIRED"
     gene            complement(962..2995)
                     /locus_tag="PLT69_01050"
     CDS             complement(962..2995)
                     /locus_tag="PLT69_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13068.1"
                     /translation="MGAEALFQRILDALPVPVFFKDRSGAYLWCNKAFERFSGRSRKD
                     LRGLRVEDLVTSNEKVRLFREKDREILENPCEQSFDVAIGNAAGDIRDVRFTRAVVAG
                     AEGTPEGIVGVMLDLTETRRAEETLRESEAKYRELFENVSDILYYHDLEGNYVDSESN
                     LSLRREWRNLVGAEVSRMNIRDLIPERLKPGFDDYLQRILENGSDSGIVAVDDAEGNR
                     HYFEYSNHLVRKEGEPVGVRGFARDITDMVMARKALAKSEEKFRSILENMEEGYFETD
                     LAGRFTFANPSMCRAAGMDMEALLKTDVFAILDDESVQSLHDAFEEAYTTGKPIRNFP
                     LVIRSGVRGAVHAEATISLVVDSAGKKAGFRGVVRDVSERVSLERALLEKNAALEEMK
                     MGLERSNEELRKAYTELKDAHTQVIKAEKLASIGQLAAGVAHEINNPIGFIMSNLNTL
                     EKYLGRLLEYLGLLEELSGQLRGEGAWETAMQRRRDLKIGTILDDVMNVISESLEGAE
                     RVKKIVHDLKSFSRSDAPEETEADLNECIESTINIVWNEIKYKARLRKDLGELPSVRC
                     YPQQINQVFMNLLVNAAHAIDGSGEIIVKSREKDGWVEVSVEDTGCGIPGENLKRIFE
                     PFFTTKEKGKGTGLGLSISYDIVRRHGGEIRVESEPGRGSTFTVRLPVHRGAS"
     gene            3411..4103
                     /locus_tag="PLT69_01055"
     CDS             3411..4103
                     /locus_tag="PLT69_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ13069.1"
                     /translation="MYWTLRTVLGSYLYIVVMIVPHVIASLFSKTGDLPHLMARRWAR
                     FTLRCSGCRVILKGVENIPQGPAVYMANHVSHFDVFALLGYLEVQFRWTVKKELFRIP
                     LLGLAMRRAGYILVDRFNHEKAMESMKRAAATIRAGTSVLIFPEGTRSCDGELKYPFK
                     KGGFHLAREAGVPIVPVTVSGTYRILPKGGAIVRPGTITIVVGKPIDPAGHDIPSLME
                     AVYTAMKLGYTL"
     gene            4107..5369
                     /locus_tag="PLT69_01060"
     CDS             4107..5369
                     /locus_tag="PLT69_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ13070.1"
                     /translation="MIDDLLYRCPLCGAFDWLDKDRCVSCSATFRLTGRSAAEMNGER
                     APLSYWYDRTRGFDLACGPDGTIAKSRRVTLSREAASGMYHGFAGITAYHFTRARIDE
                     GTLTLTPSSLTFAGLAARMTIPMEGILSLTIESNTLIVVGAGHGALFFDFHEESGKKW
                     EDLLRKTLERHYEGDEIVEFYPRVRLRSGITRTPRTEAPARELRAPARRWYRKDTSLL
                     ETILKPLGKFLVRALLTVEVRGVENIPKSDPAVILPNHESFLDSVILRFLTPRTIWFM
                     AKNSEYRGAFMKWFLRHASTFPVRRYTIDVLAVRNAVRVVSSGNLLGIFPEGERTWDG
                     RMLPIRMGAARLVLALGVPVIPVGISGAYDLMPRWTHSIRRADVRVAIGEPLNLPKIP
                     VSRQTASDIDAASRAIREAIERLRGGSA"
     gene            5366..6985
                     /locus_tag="PLT69_01065"
     CDS             5366..6985
                     /locus_tag="PLT69_01065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOJ13071.1"
                     /translation="MNRVKVIPLGGVREFGLNSMVVRTSAGAVLIDAGLMFPKFNSTG
                     IEQLIPDFSYVVENQEAFQGIVLTHGHEDHIGALPYLLREAPIPVYGHPFALELARRK
                     LRECDADVRARLVPVYPGDRVELGGMEIAFTEVEHSTIGCYSLCMRTPQGIVVHSGDF
                     KTLPPTGFCPWGAPVELFVCDSTNAEIDPRNADEKVVCSCIEDIVKNTEGAVVVSTFS
                     SNVMRIRMLYEIAVTHNRKVTIIGRSLTQVVDIATDLGLLELESRDLVKPEEITTMDR
                     SRLMVICTGTQGEMYSVLSLIARGWHRFKVRSGDSVIISARIIPGNEIAIGRCIDQLI
                     DYGARVYYPDVAPVHATGHATHHQLVEAVKSLSPRIVMPVHGELRHMKALAELVSGSP
                     GARPEFVFARAGQEWSLADGRMELTGEVPHGKCFVDGELTGDIKDVVLRDRRHISEGG
                     LIVVFTVLDSANAEIVLGPDIMCKGVVPASMEAEIVEEIKQVAASALEASLTRDTDTQ
                     VAKNRVRESIGSMIKARLGKKPMIIPVIMEL"
     gene            7233..>9298
                     /locus_tag="PLT69_01070"
     CDS             7233..>9298
                     /locus_tag="PLT69_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOJ13072.1"
                     /translation="MPAKRERASDLPLHIIGLVLLFCAVFVFLSLVSYSPDDPGFSLS
                     RKADEVRNWMGFVGSYLADGLYLLVGVWSFSLPAFMVDYAYRYCLRKRQVRWLHPSST
                     AVISFAVLGMLHLAFPGGVGFIDPGMKAGGLLGKIIAESLTAYLNYWGSVVLLLMLLA
                     GGIMLGYEPRSALNALVASWAGLRERMARRRALTQPARPKPKRSGQDTGQAQTPARQE
                     GVKEPRITLSDNEPAVALEVPKQVTLDFIENYQLPSLSLLSNPPSRGRTITEKEFRAN
                     ASLILSKLKDFGVEGEILEIRPGPVITMYEFTPAPGIKINKIVSLADDLAMALKASPV
                     RVVAPIPGKDAVGIEIPNRTREIVGLRRILASKEFTTSTAPLTLALGTDIEGTPVVAN
                     LAKMPHLLIGGATGMGKSVGLNAMILSLLYKNNPSELKFIMIDPKRIELSHYEGIPHL
                     IHPVVTDPRHALPVLKWAVHEMEQRYALFKEHGLKGIDSYNKKIRKDDPNAGSTLACL
                     ANEDGTAPLGVLPKIVIVIDEMADLMMVNREVEVYIARLAQMARAAGLHLIVATQRPS
                     VDVITGLIKSNFPSRISFRVSSKIDSRTILDSSGADQLLDNGDMLLLTPGTTHLKRIH
                     GAYVSEEEIEKVISFIKEQQEPEYVREIDTHIEELAQDDGLTGADPGYDPMYDEALEF
                     VTSKGTA"
BASE COUNT         1677 a         3105 c         2819 g         1697 t
ORIGIN      
        1 ccgccacgcc acccccggct accaggtcca gggccgccca cgggtcgtcg aggaccgtga
       61 catcgagccc cgtgtcagca aacaggcgcc tgatggccct caggatgtgc tcgtcgtcgt
      121 ccaggaatac cacctttcct tccatctcag tcctctctga tcgccgcgat caccacgcct
      181 tccctcttcg agggagatcc cagctcgttc cccatgaggg tgacgggaga tccgtacagc
      241 tcccctctgc acctcgccga gcccttctcg atcacgaggc cgcaaaacga gttgatgacc
      301 tcgggcaacg cgtcgcgcct gtccatcccg atgagccccc tcccgccgac gccgaggatc
      361 tcccgcgcgg ccctgttcgc ctggacaacg atcccgtccc cgtcgacgcc aacgaccgcg
      421 agggggaggc tgtcgaggat ctcctgcgcc ctcatgagga tcctgttctg gacgaggagt
      481 tcctgggtct gctgttccac catggcctcg aggttctcgt tcaagacctt gagttcgtcg
      541 ttggccctct gaagatccct gttcagcctg gcgttctccg tggcgagcct gtagtgttcc
      601 acggcgttgt gaaccgtgtg cttcaaatcg cgatcgttcc agggctttgc catgaacttg
      661 tagatgccgg cctcgttgac cgcgtccacc accgccgcgg tgtccgcgta gccggaaagc
      721 acgatcctca cggaatcggg ccacctcctg cacacctctt tcaggaaatc aaccccgttg
      781 atcccgggca tgcggtagtc tgcgatcacg acctgcaccg gctgcacctc gcttaggatc
      841 ctgagcccgt cctcgacgct cgtcgcggtg agcacctcgt agtcttcgtc gatgaagacc
      901 ctccgtatcg cgctgagcac gttcggctcg tcgtccacgc agagaattcg agcaaggccg
      961 ctcatgaagc ccccctgtgc acgggcagcc tcaccgtgaa ggtcgacccc ctgcccggct
     1021 cgctctcgac gcgtatctcg ccgccgtgcc gcctcacgat gtcgtaggag atggacagcc
     1081 cgagccctgt ccccttgccc ttttccttgg tggtgaagaa cggctcgaag atcctcttca
     1141 ggttttcccc cgggatcccg catccggtgt cctcgacgct cacctctacc cagccgtcct
     1201 tttccctcga cttaacgatg atctccccgc tgccgtctat ggcgtgcgcg gcgttcacga
     1261 gcaggttcat gaagacctgg ttgatctgct gggggtaaca cctcacggag gggagctcgc
     1321 cgaggtcctt cctgagacgc gccttgtact tgatctcgtt ccagacgatg ttgatcgtgc
     1381 tctcgatgca ctcgttcagg tccgcctccg tctcctcggg ggcgtcgctg cgagagaagc
     1441 tcttgaggtc gtggacgatc ttcttgaccc tctccgcacc ctcgagggac tcggagatca
     1501 cgttcatgac gtcatcgagg atcgtgccta tcttgagatc ccgcctcctc tgcatggctg
     1561 tctcccacgc accctccccc cggagttgcc cggagagctc ctcgaggagc cccaggtact
     1621 cgaggagcct gcccaggtac ttctcgagcg tgttcaggtt gctcatgatg aacccgatgg
     1681 ggttgttgat ctcgtgggca acccctgcgg cgagctgccc gatggacgcg agcttctccg
     1741 ccttgatcac ctgggtgtgc gcatccttga gctccgtgta tgccttgcgc agttcctcgt
     1801 tggaccgctc gaggcccatc ttcatctcct cgagcgcggc gttcttctcc aagagggccc
     1861 gctcgagcga gaccctctcg ctcacgtccc tcaccacccc cctgaagcca gccttttttc
     1921 ccgcactgtc cacgacgagg gagatcgtcg cctcggcgtg tacggcgcct ctcacccctg
     1981 acctgatcac caggggaaag ttcctgatcg gcttcccggt cgtgtaggcc tcctcgaagg
     2041 cgtcgtggag agactgcacg ctctcgtcat cgaggatcgc gaacacgtcc gtcttcagca
     2101 gggcctccat gtccatgccc gcggcccggc acatggaggg gttcgcgaag gtgaacctgc
     2161 ctgcgaggtc cgtctcaaag tatccctctt ccatgttctc gagtatcgag cggaacttct
     2221 cctcgctctt ggcgagcgcc ttccttgcca tgaccatgtc cgtgatgtcg cgggcgaacc
     2281 cgcgcactcc gacgggctcg ccttcctttc gcacgaggtg attcgagtac tcgaagtagt
     2341 ggcggttccc ctctgcatca tccaccgcaa cgatgcccga gtcgctgccg ttctcgagga
     2401 tcctttgcag gtagtcgtcg aagccgggtt tcagtctctc ggggatgagg tcccgtatgt
     2461 tcatccggga gacctcggcg cccacgaggt tcctccactc gcgcctgagg gagaggttgc
     2521 tctcggagtc cacgtagttg ccttccaggt cgtggtagta gaggatgtcc gacacgttct
     2581 cgaagagctc ccggtatttc gcctcgctct ccctcagggt ctcctccgcg cgcctggtct
     2641 cggtgagatc cagcatgacg ccgacgatcc cttccggggt cccctcggcc cccgccacaa
     2701 cggcacgtgt gaacctgacg tcgcggatgt cacccgccgc attcccgatc gccacatcga
     2761 acgactgctc gcaggggttc tcgaggatct cccggtcctt ctccctaaac aggcgcacct
     2821 tctcgttcga cgtgacgagg tcctcgaccc tcagccccct gaggtccttt ctcgaacggc
     2881 ccgagaaacg ctcgaacgcc ttgttgcacc agaggtacgc gcccgagcga tccttaaaga
     2941 acaccggcac cggcagggcg tccaagatcc gctggaacaa ggcttcagcg cccactgcag
     3001 gcgggccatc gctgcatccg gtcgcaggat tcgaagagat cgtttcttca ccatggatgt
     3061 caggtgggtg tttcatgaat aacgtccctc tcgtgtcgta ccgccggtgt cctgccttct
     3121 cctcgacacg ttccacgtcc gcgggccttt gaagcctcac gcccctgcct gcccgagacg
     3181 ccaccggcag cgcggggtca tcgaggagac ccgtgggaca cctccgtctt cttctccctt
     3241 cctggtatat cgtcacctcc ggcctgcctc ttgagcatca tatccctgag aaaagcaagc
     3301 ccatcaccct gtccggccga gggctcaggg tggggtcggt accaccgggt gccccccgaa
     3361 gggaccccat ccctttgaca tctttgttaa agttatgtaa aacatacgcc atgtattgga
     3421 cgctcaggac agtcttgggt tcgtacctct acatcgtggt catgatcgtg ccgcacgtga
     3481 tcgcctccct gttcagcaaa acgggcgacc tccctcacct catggcgaga cggtgggccc
     3541 gcttcaccct caggtgttcg ggatgcaggg tgatcctcaa gggcgtcgag aacataccgc
     3601 aagggccggc ggtctacatg gccaaccacg tgagccactt cgacgtgttc gcgctccttg
     3661 ggtacctcga ggtgcagttc cgctggaccg tgaagaagga gctcttccgc atacccctct
     3721 tggggctcgc catgaggagg gcgggctaca tcctcgtgga caggttcaac cacgagaagg
     3781 ccatggagag catgaagagg gccgccgcca cgatacgtgc cggcacctcc gtcctcatct
     3841 tccccgaggg gacgcgatcc tgcgacggcg agctgaaata cccattcaag aagggcggct
     3901 tccacctggc cagggaggcg ggagtgccca tcgttccggt cacggtcagc ggaacgtacc
     3961 ggatcctccc caaggggggc gcgatcgtca ggccggggac catcaccatc gtggtgggca
     4021 agcccatcga cccggccggc cacgacatcc cgtcgctcat ggaggcggta tacacggcca
     4081 tgaaactcgg ttatactctt tgaccgatga tcgatgacct gctctaccgc tgccccctct
     4141 gcggcgcttt cgactggctc gacaaggaca ggtgcgtttc gtgcagcgcc acgttcaggc
     4201 tcacggggag gtcggcggcc gagatgaacg gcgagcgggc ccccctgtcg tactggtacg
     4261 acaggaccag agggttcgac ctcgcgtgcg gccctgacgg cacgatcgcg aagagccgcc
     4321 gcgtcacgct ctcgagagag gccgcaagcg gcatgtacca tggcttcgcc ggcatcaccg
     4381 cgtaccactt cacgagggcg aggatcgacg aggggaccct gacgctcacc ccctcgagcc
     4441 tcaccttcgc cgggctcgcc gcccggatga ccatccccat ggagggcatc ctctccctca
     4501 ccatcgagtc caacaccctc atcgtggtgg gtgcggggca cggcgccctc ttcttcgatt
     4561 tccacgagga gtcgggcaag aagtgggagg acctcctgag aaagaccctc gagcgccact
     4621 acgagggaga cgagatcgtg gagttctacc cgcgggtgcg gttgagatcc ggcatcaccc
     4681 gcacgccgcg cacggaggct cccgcaagag agctccgcgc gcccgcgagg agatggtaca
     4741 ggaaggacac gagcctgctc gagacgatcc tgaaacccct ggggaagttc ttggtcaggg
     4801 cgctgctcac cgtggaggtg aggggggtgg agaacatccc gaagagcgac ccggccgtca
     4861 tcctccccaa ccacgagtcg ttcctcgact cggtgatcct gcgcttcctg acgcccagga
     4921 ccatctggtt catggccaag aactcggagt acaggggcgc tttcatgaag tggttcctca
     4981 ggcacgcatc gacctttccc gtcaggaggt acaccatcga cgtgctggcc gtgcgcaacg
     5041 ccgtcagggt ggtctcctcg ggcaacctcc tcggcatctt ccccgagggc gagcgcacct
     5101 gggacgggag gatgctcccg atccgcatgg gcgcggcgag gctcgtgctg gcgctcgggg
     5161 tcccggtgat cccagtgggt atatcgggcg cctacgatct catgccccgg tggacccact
     5221 ccatccgcag ggccgatgtg cgggtcgcga taggagagcc tctgaatctc cccaaaatac
     5281 cggtttcgag gcagaccgca tccgacatag acgcggcgtc ccgcgcgatc agggaagcga
     5341 tcgagcgcct tcgcggggga tcggcgtgaa ccgagtgaag gtcattcccc tggggggcgt
     5401 cagggaattc ggcctcaact cgatggtcgt ccggacctcg gcaggcgccg tgctcataga
     5461 tgcggggctc atgttcccca agttcaactc caccgggatc gaacagctca tccccgactt
     5521 ctcgtacgtg gtggaaaacc aggaggcgtt ccaggggatc gtgctcaccc acggccacga
     5581 ggatcacata ggagcactgc cgtacctcct cagggaggct ccgatcccgg tctacggcca
     5641 cccgttcgcc ctcgagctcg ccaggcgcaa gctgagggag tgcgacgctg acgtgcgggc
     5701 gcggctcgtg ccggtgtacc ccggcgacag ggtggagctg ggggggatgg agatcgcctt
     5761 caccgaggtc gaacactcga ccatcggctg ctacagcctc tgcatgagaa cgccgcaggg
     5821 catcgtggtc cactcaggag acttcaagac cctgccgcct accggcttct gcccctgggg
     5881 cgcgcccgtg gaactcttcg tctgcgacag caccaacgcc gagatagacc ccagaaacgc
     5941 ggacgaaaag gtggtctgtt cctgcatcga ggacatcgtg aagaacaccg agggcgccgt
     6001 ggtcgtctcg acgttctcca gcaacgtgat gcgcatcagg atgctctacg agatcgccgt
     6061 gacgcacaac cgcaaggtca ccatcatcgg gaggagcctc acccaggtgg tggacatagc
     6121 gaccgacctg ggactcctgg agctcgagtc gcgcgacctc gtgaagccgg aagagatcac
     6181 gaccatggac aggtcgaggc tcatggtcat ctgcaccggc acccaggggg agatgtactc
     6241 ggtcctctcg ctgatcgcga gggggtggca ccggttcaag gtgcgctcgg gcgacagcgt
     6301 gatcatctcc gcgcgcatca tccccgggaa cgagatcgcc ataggcaggt gcatcgacca
     6361 gctcatcgac tacggggcga gggtctacta cccggacgtg gcgccggtgc acgccaccgg
     6421 tcatgccacg caccaccagc tcgtggaggc cgtgaaatcc ctctccccca ggatcgtcat
     6481 gccggtccac ggcgagctca ggcacatgaa ggcgctcgcc gagctcgtct ccggctctcc
     6541 tggagcgaga cctgagttcg tgttcgccag ggcgggccag gaatggtccc tcgccgacgg
     6601 caggatggag ctcaccggcg aggtccccca cggcaagtgc ttcgtggacg gcgaactcac
     6661 gggcgacatc aaggacgtgg tgctgaggga ccgcaggcac atatcggaag gcggcctcat
     6721 cgtcgtcttc acggtgctcg actcggcgaa cgcggagatc gtcctgggcc cggacatcat
     6781 gtgcaagggc gtggtgccgg cgagcatgga ggccgagatc gtagaggaga tcaaacaggt
     6841 cgcggcctcg gccctcgaag cctccctcac gagggacacc gacacgcagg tggcgaagaa
     6901 ccgggtgcgg gagtccatag ggtccatgat caaggccagg ctcggcaaga agcccatgat
     6961 catccctgtg atcatggagc tgtgagcggg ctctcctcca cgggctggga aagacggcgc
     7021 tcgagggggg catgcccatg atgcgacacc cgaaaccctc cgggaccgag cgccatgacg
     7081 accgacggac aatcgcctcc ggagaacgcc cgctcatcga gaggactttg acttcttcat
     7141 gatttctggt atggacgata cgagggcttt gaattacagc atgatgcagg aaatgcatcg
     7201 ataggcggga aaccgggcaa aaggggaggg tcatgcctgc aaaaagggag cgcgcaagcg
     7261 atctgcctct tcacatcatc gggctcgtgc tcctcttctg cgccgtcttc gtgttcctct
     7321 cccttgtgtc gtactcaccg gacgacccgg gtttctcgct ctccaggaag gcggacgagg
     7381 tccgcaactg gatggggttc gtgggctcgt acctggccga cggcctgtac ctcctcgtgg
     7441 gcgtctggtc gttttccctg cccgccttca tggtggacta cgcgtaccgg tattgcctca
     7501 ggaaaagaca ggtccggtgg ctgcacccct cgagcacggc ggtcatatcg ttcgcggtgc
     7561 tcggcatgct gcacctggcg ttccccgggg gcgtcgggtt cattgacccc gggatgaagg
     7621 caggcggcct cctgggaaag atcatcgcgg agtcgctcac ggcatacctc aactactggg
     7681 ggtcggtcgt cctgctcctc atgctgctgg ctggcggcat catgctcggg tacgagccga
     7741 ggtccgcgct gaacgcgctc gtcgcatcct gggccggcct gagagagcgc atggcaagaa
     7801 ggcgggcctt gacgcagccg gcgcgcccca agccgaagag gtcaggccag gacaccggcc
     7861 aggcacaaac cccggccagg caggaggggg tcaaggaacc ccggatcacg ctgtcggaca
     7921 acgagcccgc cgtcgccctc gaggtaccga aacaggtgac cctcgatttc atagaaaact
     7981 accagctccc ctccctctcc ctgctctcca accccccctc gcgggggcgc accatcacgg
     8041 agaaggagtt cagggcgaac gcgtccttga tcctgtcaaa gctcaaggac ttcggcgtgg
     8101 aaggcgagat cctcgagatc aggcccgggc cggtgatcac catgtacgag ttcacgcctg
     8161 caccgggcat caagatcaac aagatcgtct cgctcgccga cgacctcgcc atggcgctca
     8221 aggcctcgcc cgtgagggtg gtggccccca tccccggcaa ggacgcggtg ggcatagaga
     8281 tacccaaccg gacgagggag atcgtgggcc tcaggaggat cctcgccagc aaggagttca
     8341 ccacgtcgac cgcgccgctc acgctcgccc tcggcaccga catcgagggc acgcccgtgg
     8401 tggcgaacct ggcgaagatg ccgcatctcc tcatcggggg cgcaacgggc atgggcaaga
     8461 gcgtggggct caacgccatg atcctctcgc tcctctacaa gaacaacccc tcggaactga
     8521 agttcatcat gatcgacccg aagcgcatcg agctgtccca ctacgagggg atcccccacc
     8581 tcatccaccc cgtggtcacg gaccccaggc acgccctgcc cgtgctcaag tgggccgtgc
     8641 acgagatgga gcaacgctac gccctgttca aggagcacgg gctcaagggc atcgactcgt
     8701 acaacaagaa gatcaggaag gacgacccga atgcgggctc gacgctcgcg tgcctggcga
     8761 acgaggacgg gaccgccccc ctgggcgtgc tcccgaagat cgtgatcgtc atagacgaga
     8821 tggcggacct catgatggtg aaccgggagg tggaggtcta catagcgagg cttgcccaga
     8881 tggccagagc ggcgggcctc catctcatcg tggcgaccca gaggccctcg gtggacgtga
     8941 tcaccgggct catcaagtcg aacttcccct cgaggatctc gttcagggtc tcctcgaaga
     9001 tcgactcccg caccatcctc gattcttcgg gcgcggacca gctcctggac aacggcgaca
     9061 tgctcctcct cacgcccggg accacccacc tcaagcggat ccacggcgca tacgtctcgg
     9121 aggaggagat cgagaaggtc atctcgttca tcaaggaaca gcaggagccc gagtacgtca
     9181 gggagataga cacccacata gaggaactcg cgcaggacga cggcctcacc ggggcagacc
     9241 ccgggtacga ccccatgtac gacgaggcgc tcgagttcgt gacgagcaag gggacggc
//
LOCUS       DAOSCR010000018         9228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_4908_length_9228_cov_3.216505, whole genome shotgun sequence.
ACCESSION   DAOSCR010000018 DAOSCR010000000
VERSION     DAOSCR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4908_length_9228_cov_3.216505"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            878..2455
                     /locus_tag="PLT69_01075"
     CDS             878..2455
                     /locus_tag="PLT69_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HOJ13073.1"
                     /translation="MNALFVYPRIPRTYWSFTHALEFLSKKAAFPPLGLLTVAAMAPK
                     GWRRRLVDLNVRELTDEDLSWADSVFVSAMSVQKASLLEVVRRARRHGVRVVAGGPLF
                     TRDMTEYPEIDHVILGEAEVSFRRFVGDLENNAAARVYEEDGKPDLSLTPPPAWELID
                     FGDYESMLLQFSRGCPFDCEFCDIVRLNGRKPRTKDAGRFVAEVEDLYRRGWRGSVFI
                     VDDNFIGNRPRVKEMLRALASWMHHRGAPFHFFTEASIDLAQDPELMDLMVMAGFNKV
                     FVGIETPVDQSLKEAGKTQNLKTDLLEAVHTIQSRGIEVMGGFIVGFDSDPEDVFERQ
                     AEFIQKSGIVMAMVGLLEALKGTRLWARLASEGRLLDEASGDNTDGSLNFIPRMDREK
                     LVRGYRRLVELVYSPAHYYRRCLSFLRTCRFGRVSKIRLNGVAAFLKTIWRIGVKNEG
                     DLRRYYWRLLAWTLLRRPRSFGEAVRLMIVGVHFRKWLDELQAHEAERAGHPRFTLHH
                     GGIPGARQGDGRHSPRA"
     gene            complement(2481..4040)
                     /locus_tag="PLT69_01080"
     CDS             complement(2481..4040)
                     /locus_tag="PLT69_01080"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ13074.1"
                     /translation="MRGPIEEIVFFGDDVRIGRFPAHTRFVYANEALAALPDFDAELR
                     AALDRPLGAEPLEKQLGPKSRVTIAFDDPCLPVPLMLRDPRAKVVGEVLKRLFTIGIP
                     KERIRIVCANGLHRKWSLDELKTVLGRKVVSLMAERISCHDATLDDELSFLGTTSRGH
                     EVEVNRACVEADMLIYVNLNFTSMNGGAKSVLVGLGSWRSIRHHHGPAQWNTDASIMD
                     PATCPMHAILAEMYPLVQKGCNVFQIETVTNNNVWPFPLDLLLAPLGSEHGRKRAGAL
                     RRTLFEAASLAPEAFKGWVRKRLVRSDYRPVAVNAGAPGPVHARTLDVLFRQQNVAVE
                     SQADVVIYGVPSLSPYSSLSDFNPILLRSLVMGYLVGLFRGRPLVKKGGVVIACNPGF
                     EQFNERHHPAYIDFWKNHLPEYEDPVRCWEELSEPYAANERYLARYREDLAYHGAHGL
                     INWMWSGMGLKHVSSVILAGAREPLTARKIGFIPEPDLDRAIDRALETAGRDASITYQ
                     VIPPLFCVDVA"
     gene            complement(4095..4964)
                     /locus_tag="PLT69_01085"
     CDS             complement(4095..4964)
                     /locus_tag="PLT69_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ13075.1"
                     /translation="MKRFDFSGKRVLITGASAGIGRALSAQFARRGAILALGSLPSEA
                     GELESWARELRRTHGTEVHTFPVDLLEEGGPERLHHEVSLRFGKVYALVNNAGTVAYG
                     EFHETDWGRQEATLRLNLLVPMRLMHLFLGDMVRSGEGVVFNVSSVSALQPTPFQAVY
                     GASKAGLQSLSQAVRAELAGSGVTVCTLNPPYIDTRLLRTPGYPEDLRFFKISGKKSP
                     EWLAERAIRAFEEGRMMFIPGLANRIIHDLLVRVSPRGVVDAVSRFFLQGASRAAAKK
                     RAGRGRGTEGTNR"
     gene            complement(5090..5527)
                     /locus_tag="PLT69_01090"
     CDS             complement(5090..5527)
                     /locus_tag="PLT69_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ13076.1"
                     /translation="MFEKVLFPTDFSDVATKALQYVKELRQAGTREVTVLHVVDQANL
                     DLLGTYSAVGDLVNLEREILKRASDEVGFVANELRQCGFTVNERVMQGSPVRLILRLA
                     EEENPSLIVVGSHGKSNLEEMLMGSVSEKVVRKARHPVLVIKR"
     gene            complement(5963..6562)
                     /locus_tag="PLT69_01095"
     CDS             complement(5963..6562)
                     /locus_tag="PLT69_01095"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOJ13077.1"
                     /translation="MASRFPATVFLALVLGMFALAGRATAEVVEGPGPVSVSGALFER
                     TLKADGSTLRLAGAGLLRYRVVFKGYAAALYLGTGHAPDAVFGDVPKRLEIEYFHAIP
                     AQDFRDATRQGIEMNVARDELGVLKPRLDRILAAYLPVKPHDRYSFTYLPGRGSTLRL
                     NGRSLVTVEGLDVANAFLKIWLGPNPADRDLKSRLLGMP"
     gene            complement(6567..8003)
                     /locus_tag="PLT69_01100"
     CDS             complement(6567..8003)
                     /locus_tag="PLT69_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ13078.1"
                     /translation="MPAPASASTRLDAVVVGSGPNGLAAAVTLAGAGRSVKVYEAADT
                     PGGAVRSGQSILQGYVHDLGAAVFPMAVDTPFFRGVPMREFGLEFIFPEASVAHPLDD
                     GTAAVLWRDPERTAAGLGPDSNRYWALMRPVLGRWDELVDDLFAPLHLPRHPAALVSF
                     GLRACVPAAVLARAVFRTGRARALLAGLAAHSILPLWHPMSAAFAVIMAASGHLPGWP
                     VVRGGAGRLSAALVSYLLSLGGEIETGREVRDLRDVEPSRAVFFDTAPARALRIAGAT
                     LPPSYGRRLQAFRHGPGAFKVDWALSGPIPWKAPACREAATVHLGGTLEEIERAEAAV
                     WRGVPPEKPFVFLVQPSLFDASRAPAGRHTAWAYCHVPNGSAADMTGPVEAQVERFAP
                     GFKDLVLARCTMGPADLELFDMNCTGGDIAGGANTFTQVLGRPVFSANPYAMPAPGLY
                     LCSASTPPGGGVHGLCGYHAARAFLEGG"
     gene            complement(8007..9017)
                     /locus_tag="PLT69_01105"
     CDS             complement(8007..9017)
                     /locus_tag="PLT69_01105"
                     /inference="COORDINATES: protein motif:HMM:NF020906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ13079.1"
                     /translation="MTDGSFHVGGERGRIVLNGTITDSASTALGDAYAAAAVVPGIEL
                     DCAGIEHIDVSGMNELIKLRLRAGAQGRKITAVGVGPELMDVFRATRTVEAFAPRAAK
                     SQVAYDKARLAAWARPIERISLSEVPETAVNLNVDGLGVVGPVQGFGQLWEKTYRVRL
                     TGSAATPPEVVQALKDNFPSLQPPQNRFFPSSRGIAPGEVVLINAHTPVGLISTGVWV
                     VYADSGSFTFMTPQGHPESGWVSFTAYEADGCTVAQVRGFARANDPVYELGFRLMGSK
                     EQERIWTHVLESLARHFGVPGWVRMTKTCVGPDLQWDQALNVWYNAQIRTMLSSFRRA
                     FS"
BASE COUNT         1536 a         3079 c         3040 g         1573 t
ORIGIN      
        1 ttttttttgt cttcgcaggg ttatgtgctc gtgtctcccc ggggccgaac catgatgccc
       61 tccgggaagc gaggcaggag aggcgctgat ctcccttcaa ggccgcacga ccaggcattc
      121 gggcgtctct cctcgagcgt gcaccgtctt gcaggactgt tcggctggcc tgcaacctca
      181 ggcggccgtc ttcgcgcgtg ccacgcatcg ccgcaggcag gtagaggtgt ggtcccaccg
      241 ggaagggcgg tgaaagcccg cccgggtcgc acgggcgcct gaggcccaga gggccgtgga
      301 tgaagaagac cggttcaaag gcttacctct ctcatcacgt cgcacgagcg cccaggacag
      361 tcgtggatga cgggtctccg gttgaaggcg gtggcgcctt tgtcatgcat gcccggcgtg
      421 cacggcggcc gacccatctc ggctcgtgca tggacggtgc gtggaccacg ggttgtccaa
      481 cccgaagagc gctcgcccga gatggttccc agcacggaaa gaagagaacc gtgcggagaa
      541 tgcgcccgat gaggagatga gggatggacc accggtgcgt cttgcctgtc ctttctgcgg
      601 gccgaccccc tcacgcctgg aacggcgggt gacagaaaga ggcagacccg gcgcaggtgc
      661 gctcgccttt ccgtccgtcg tatccaaaag gaatgggggg aagaggtgcg agggatgaga
      721 acccctgtca tgtcagcctg ttttcaaaat gttgacaaac tctttgaggt acttttgcac
      781 tcgtttgcgc ctggcatgtg ctatggtctc ctccattgaa gggaatgcga tcctcaggtc
      841 gtgaggcatg gactggagac aaaggaggga aggaaagatg aacgcgctct tcgtataccc
      901 gcggatcccg cgcacgtact ggagtttcac ccatgcactc gaatttctct cgaagaaggc
      961 cgcgttcccc ccgctcggac tcctgacggt cgcggccatg gcgcccaagg ggtggaggag
     1021 gaggctcgtg gacctgaacg tccgcgagtt gaccgacgag gacctctcgt gggcggactc
     1081 ggtcttcgtg agcgccatga gcgttcagaa ggcgtctctc ctcgaggtcg tcaggagagc
     1141 gcggcggcac ggggtgaggg tggtggccgg aggccccctg ttcaccaggg acatgaccga
     1201 gtacccggag atcgaccacg tgatcctcgg ggaggcggaa gtgagcttca ggcgtttcgt
     1261 gggggacctg gagaacaacg ccgcggcgag ggtctacgag gaggatggga agccggacct
     1321 gtcgctgacc ccacctccgg catgggagct catcgacttc ggcgactacg agtccatgct
     1381 gttgcagttt tccaggggct gtccctttga ctgcgagttc tgcgacatcg tgaggctcaa
     1441 cggcaggaag ccccgcacca aggacgccgg ccggttcgtg gccgaggtgg aggatctcta
     1501 ccggaggggc tggaggggat cggtcttcat cgtggacgac aacttcatcg gcaacaggcc
     1561 cagggtcaag gagatgctca gggcgctcgc ctcgtggatg caccaccgcg gcgcgccctt
     1621 ccacttcttc acggaggctt ccatcgacct cgcccaggat cccgagctca tggacctcat
     1681 ggtgatggcc gggttcaaca aggtgttcgt cggcatcgag acccccgtcg accagagcct
     1741 gaaagaggct ggcaagaccc agaacctgaa gacggatctc ctcgaagcgg ttcacaccat
     1801 ccagtccagg gggatcgagg tcatgggcgg gttcatcgtg gggttcgaca gcgacccaga
     1861 ggacgtgttc gaacgccagg ccgagttcat ccagaagagc ggcatcgtca tggccatggt
     1921 ggggctcctc gaggcgctca agggaacgag gctgtgggcc aggctcgcct cggagggcag
     1981 gctcctggac gaggcgtcgg gggacaacac cgacggttcc ctcaacttca tcccgcgcat
     2041 ggacagagag aagctggtga gggggtacag gaggctcgtc gagctcgtct actcgccggc
     2101 ccactactac cggaggtgct tgtctttcct tcggacatgc aggttcgggc gggtctcgaa
     2161 gatccgcctc aacggcgtgg ccgccttcct gaagaccatc tggcgcatag gcgtgaagaa
     2221 cgagggagac ctcaggaggt attactggag gctcttggcc tggacgctcc tcaggcggcc
     2281 gcgttccttc ggcgaggcgg tgaggctcat gatcgtgggc gtgcacttca ggaagtggct
     2341 cgacgagctc caggcgcacg aggccgaaag ggctgggcac ccacggttca ccctgcacca
     2401 tggaggcatc ccgggcgccc ggcaaggcga cgggcgccac tctccccgcg cgtgatcgaa
     2461 acggcacggc ctgccgtctt tcaggcgacg tcaacacaga agagcggggg gatgacctgg
     2521 taggtgatgc tggcatccct gcccgccgtc tcgagtgcgc ggtctatcgc gcggtcgagg
     2581 tcgggctccg ggatgaagcc tatctttctc gccgtgagcg gttccctggc gcccgccagg
     2641 atcaccgaac tcacgtgctt gagacccatg ccgctccaca tccagttgat gagcccgtgg
     2701 gccccgtgat aggcgaggtc ttcccggtag cgggcgaggt acctctcgtt tgccgcatag
     2761 ggttccgaga gctcctccca gcacctgacc gggtcttcgt actcgggaag gtggttcttc
     2821 cagaaatcga tgtaggccgg atggtggcgc tcgttgaact gctcgaagcc ggggttgcag
     2881 gcgatcacca ccccgccttt cttcacgagc ggcctgcccc tgaacagccc caccaggtag
     2941 cccatgacga gagacctgag gaggatgggg ttgaagtcgg acagggagct gtacgggctc
     3001 aagctcggca ccccgtagat caccacgtcc gcctgagact cgacggccac gttctgctgc
     3061 cggaagagga cgtctaaggt ccgtgcgtga accggcccgg gcgcccccgc gtttaccgcc
     3121 accggcctgt agtccgagcg cacgaggcgt ttcctgaccc agcccttgaa cgcctcaggc
     3181 gcgagcgacg ccgcttcgaa gagcgtcctt ctcaacgccc ccgcccgttt ccggccatgc
     3241 tcggacccga gaggcgccag caggaggtcg agggggaacg gccagacgtt gttgttcgtc
     3301 accgtctcga tctggaagac gttgcagccc ttctgcacga gggggtacat ctcggcgagt
     3361 atcgcgtgca tcgggcaggt ggccgggtcc atgatggagg cgtccgtgtt ccactgcgca
     3421 ggcccgtggt ggtgccttat gctcctccac gaaccgagcc ccaccagcac gctctttgcg
     3481 ccaccgttca tgctcgtgaa gttcaggttc acgtatatga gcatgtccgc ctcgacgcac
     3541 gccctgttga cctccacctc gtgccccctg gaagtggtgc cgaggaagga cagttcgtcg
     3601 tcgagggtgg cgtcgtggca cgagatcctc tccgccatga gggagaccac cttccggccc
     3661 aggacggtct tcagctcgtc caggctccac ttgcggtgaa gcccgttggc gcagacgatg
     3721 cggatcctct ccttggggat gcctatggtg aagagccgct tgaggacctc cccgaccacc
     3781 ttcgccctgg ggtcgcgaag catcaggggg acgggaaggc aggggtcgtc gaaggctatg
     3841 gtgacgcggc tcttcgggcc cagctgtttc tccaggggtt ccgcgccgag tgggcggtcg
     3901 agcgcggctc ggagttcggc gtcgaagtcg ggcagggccg caagcgcttc gttcgcgtac
     3961 acaaacctcg tatgagccgg gaacctccct atgcgaacat cgtcgccgaa aaagacgatc
     4021 tcctcgatgg gccctctcat cacccacgcc cccttccggc ctaattcttc aggttgccgt
     4081 caatgggagt ttcttcatcg gttcgtgccc tcggtccccc ggcctcttcc cgcccttttc
     4141 tttgccgccg cccgggacgc gccctgcagg aagaaccgag acaccgcgtc caccacccct
     4201 ctcggcgaga cccgtacgag caggtcgtgg atgatcctgt tcgcaagccc cgggatgaac
     4261 atcatcctgc cctcctcgaa ggcgcgtatg gccctctcgg cgagccactc agggctcttc
     4321 ttgccgctga tcttgaagaa cctgaggtcc tcggggtagc cgggcgtcct gaggaggcgc
     4381 gtgtcgatgt agggcgggtt cagggtgcac acggtgaccc cactccccgc gagctccgcg
     4441 cggaccgcct gggagaggct ctgcagtcct gccttgctcg ctccgtagac ggcctggaac
     4501 ggggtcggct ggagcgcaga gacgctcgac acgttgaaca cgaccccttc gcctgagcgc
     4561 accatgtccc cgaggaagag gtgcatgagg cgcatgggca cgaggaggtt gagcctcagc
     4621 gtcgcctcct gccgccccca gtccgtctcg tggaactcgc cgtatgcgac agtgcccgcg
     4681 ttgttcacca gggcgtacac cttcccgaag cgcaggctca cctcgtggtg gagcctttcc
     4741 gggcccccct cctccagcag gtcgaccggg aaggtgtgca cctctgtgcc gtgggttctg
     4801 cgcagctccc gtgcccagga ctcgagctcg ccggcctcgc tgggaagaga acccagggcg
     4861 aggatcgccc cccgcctcgc gaactgcgcc gagagcgccc tgcctatgcc ggccgaggcg
     4921 ccggtgatga ggaccctctt tccggaaaag tcgaaccgct tcatcgtcct cctccctccg
     4981 tccaggcagc ccttgttgca tccccgatcc atccgcacgt actcgcgccc atgaagcggc
     5041 agttgcacca ggcctgcctg ctggttcggg ttcgccggtg cactcgatct cagcgtttga
     5101 tcacgaggac ggggtgtctc gccttccgca ccaccttctc ggagaccgag cccatgagca
     5161 tctcctcgag attgctcttc ccatgcgacc ccacgacgat gagggagggg ttctcctctt
     5221 ccgcgagcct caggatgagc ctcacgggcg aaccctgcat cacgcgctcg ttcacggtga
     5281 agccgcactg tctcagctcg ttggccacga atcccacctc gtcggatgcc cgcttgagga
     5341 tctccctctc gaggttcacg aggtccccga ccgcgctgta ggtgcccagg agatcgaggt
     5401 tggcctgatc cacgacgtgg agcaccgtga cctcgcgggt gcccgcctgc ctgagctcct
     5461 tgacgtattg gagggccttg gtcgcgacat cggagaaatc ggtggggaac aggaccttct
     5521 cgaacatggc aggcctcctt atgtacgtaa ttgagtgtgc caagaaacat agtaatgtct
     5581 tgtcgcaccg tgtcaacgct cgggaccggc cgtgccgtgt caagactcgt tcggaacgtc
     5641 cccttgctca cctcgttcac aatgtcccct gcccgtacgt gtacgtgagc accgtgtttc
     5701 agtcatggga gggaccacga ccaatccgta ggggaggggc cgccgccttc acgctcgacc
     5761 cgctcggctc cttccaccca aaaatccccg accgctcccg cttcttcccg ctcatcaaga
     5821 tcgcccccat ggcccctcca ggggaccgtc ccgccaaaac cctctcaagg ggacattcct
     5881 ctcgagccga aaggggacat tcgtgaaggg tcgggacata caccgtgagg taacatgtct
     5941 gcaaatgctg taagagaact gttcagggca tgcccaggag ccgactcttc aggtccctgt
     6001 ccgccgggtt gggcccgagc cagatcttca ggaaggcatt ggccacatcc aggccctcca
     6061 ccgtcacgag gctgcggccg ttgagccgga gcgtggaacc ccgccccggg aggtaggtga
     6121 aggaatagcg gtcgtggggc ttcacgggga ggtacgccgc caggatcctg tcgaggcggg
     6181 gcttcaggac gccgagctcg tctctggcca cgttcatctc gatgccctgc ctcgtggcgt
     6241 cccggaagtc ctgtgcgggg atggcgtgga agtactcgat ctccaggcgc ttgggcacgt
     6301 cgccgaacac ggcgtccggg gcatgccccg tgcccaggta gagggccgcc gcatacccct
     6361 tgaacaccac ccggtagcgc aggagccccg cccctgccag gcgcagggtc gatccatccg
     6421 ccttcagggt cctctcgaac agcgcgcccg agacggacac cgggccgggg ccctcgacca
     6481 cctccgccgt cgcgcggccg gcaagcgcga acatgcccag gacgagggcg aggaaaaccg
     6541 ttgcggggaa acgggacgcc acggcgtcac cctccttcca ggaaggccct ggcggcatgg
     6601 tagccgcaca gcccgtgcac gccgccgccc ggcggggtgg aggccgagca gaggtacaac
     6661 ccgggtgcgg gcatggcata gggattggcg ctgaacacgg gcctgcccaa gacctgcgtg
     6721 aacgtgttgg ccccgcccgc gatgtctccg cccgtgcagt tcatgtcgaa gagctccagg
     6781 tccgcgggtc ccatggtgca ccgggccagg accaggtcct tgaagcccgg ggcgaagcgc
     6841 tccacctggg cctcgacggg tccggtcatg tccgcggcag acccgttggg aacgtggcag
     6901 taggcccacg cggtgtgcct gcccgccggc gcccgggagg cgtcgaacag gctcggctgc
     6961 accaggaaca cgaagggctt ctccggtggg acgccccgcc acaccgccgc ctcggcgcgc
     7021 tcgatctcct ccagggtgcc gcccaggtgc accgttgccg cctccctgca ggcgggggcc
     7081 ttccacggga tggggcctga aagcgcccag tccaccttga aggcgccggg gccgtgccgg
     7141 aaggcctgga gcctgcgccc gtaggacggc ggaagcgttg ctcccgcaat ccgcagcgcg
     7201 cgtgcgggcg ccgtgtcgaa gaacactgcc cgcgacggtt ccacgtccct caggtctctg
     7261 acctcgcggc ccgtttcgat ctccccgccc agggaaagga ggtaggacac cagcgctgcg
     7321 gagagcctcc ccgccccgcc ccgcacgacg ggccagcccg gaaggtggcc gctggccgcc
     7381 atgatgacgg cgaaggccgc gctcatgggg tgccacagcg gcaggatgga gtgtgccgcg
     7441 agccctgcca gcagggcccg ggcgcgcccg gtccggaaca ccgcccgggc gaggacggct
     7501 gcggggacgc acgcccgcag gccgaaggaa acgagcgctg ccgggtggcg ggggaggtgg
     7561 agcggcgcga aaaggtcgtc caccagttcg tcccatcgcc ccagcaccgg ccgcatgagg
     7621 gcccagtacc ggttggagtc gggcccgagg ccggcggcgg tcctctccgg gtcgcgccac
     7681 aggaccgccg ctgtgccgtc gtccagggga tgggccacgg aggcctccgg gaagatgaac
     7741 tccaggccga actccctcat gggcacgccc cggaagaacg gggtgtccac ggccatgggg
     7801 aagaccgctg cgcccaggtc gtgcacatac ccctgaagaa tggactggcc ggacctcacc
     7861 gccccgcccg gggtgtccgc cgcctcgtac accttcaccg agcggcccgc cccggccagg
     7921 gtgactgccg cggccaggcc gttgggtcct gaccccacca cgaccgcgtc caggcgcgtg
     7981 ctggcgcttg caggggcggg catggcctag gaaaaggccc tcctgaagga cgagagcatg
     8041 gtccgtatct gggcgttgta ccagacgttc agcgcctggt cccactggag gtcgggcccc
     8101 acgcaggtct tggtcatgcg cacccacccg ggcacgccga agtgcctggc cagggactcc
     8161 aggacatggg tccagatgcg ctcctgctcc tttgacccca tgagccggaa tcccagctcg
     8221 tagaccgggt cgttggcccg ggcgaagccc ctcacctgtg ccacggtgca gccgtcggcc
     8281 tcgtacgcgg tgaagctcac ccagccggac tcggggtgcc cctggggggt catgaaggtg
     8341 aaggacccgc tgtccgcata caccacccac acgccggtgc tgatgagccc cacaggggtg
     8401 tgggcgttga tgagcaccac ctctcccggg gcgatgcccc tgcttgaagg gaagaagcgg
     8461 ttctggggcg gctgcaggct ggggaagttg tccttcaggg cctgcacgac ctcgggaggg
     8521 gtggcggcgg acccggtgag cctcacccgg taggtcttct cccagagctg gccgaaaccc
     8581 tgcaccgggc ccaccacgcc gagcccgtcg acgttgaggt tcaccgcggt ctcgggtact
     8641 tccgacaggg atatcctctc gatgggcctg gcccaggcgg caaggcgtgc cttgtcgtag
     8701 gccacctgag acttcgcggc ccggggcgca aaggcctcca cggtcctggt ggcccggaag
     8761 acatccatga gctccggccc caccccgacg gccgttatct tcctcccctg ggcgcctgcg
     8821 cggagcctga gcttgatgag ttcgttcatc ccggagacgt cgatgtgctc gatgcccgcg
     8881 cagtccagct cgatgccggg gaccacggcg gccgcggcat acgcgtctcc cagcgctgtc
     8941 gaggccgagt ccgtaatggt cccgttcagg acgatgcgcc ccctttcccc tccgacatgg
     9001 aacgatccat ccgtcatggt actcctcctg ttcatggaga gaacgtatat cgggccgttc
     9061 gtccggccag gccccaccac tatagggctt ctgtgccccc gagacaaccg ccccgttgtg
     9121 cctgcgcccc cgatgccgcc ccctgcgatg cgccttcgcc gatggtgacg atccccaggt
     9181 tgaaggactt gccgcccacc cggcggacac ccgctttagc gacggcgt
//
LOCUS       DAOSCR010000019         9055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5030_length_9055_cov_3.518444, whole genome shotgun sequence.
ACCESSION   DAOSCR010000019 DAOSCR010000000
VERSION     DAOSCR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5030_length_9055_cov_3.518444"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLT69_01110"
     CDS             complement(<1..481)
                     /locus_tag="PLT69_01110"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOJ13080.1"
                     /translation="MTDVDDRSIISPMARTPSRQTIIEEGARIVHARGFRAAGLKEIL
                     DAAGVPKGSFYFHFKSKEDFGLALVDHFQSLIESAGEKLLGDQGTPPLERLARFMDAY
                     ENMFEKMGFSRGCPIGNLMQEMSDVSEAFRERVARAYEGMHAAIQAVLDEAKGLGQVA
                     "
     gene            598..2265
                     /gene="pgm"
                     /locus_tag="PLT69_01115"
     CDS             598..2265
                     /gene="pgm"
                     /locus_tag="PLT69_01115"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOJ13081.1"
                     /translation="MAVHDLAGKPVPRSMLVDTASLVDAYYRTRPDPAHPGCRVSFGT
                     SGHRGSSIAASFNEDHILAITQAICDYKREFGITGPVFVGRDTHALSEPAFKTAIEVL
                     AANALTAMVDEDLGFTPTPSVSRAVIEYNRSRSSDPADGIVITPSHNGPEDGGFKYNP
                     PEGGPADTKTTGWIEKRANALLEGRLAGVRRIPYERALSAPTTTRYDFTGPYVEGLAS
                     VIDMGSIASSGLRLCADALGGSGASYWHRIAERYALSIDVLHDTADATFGFMTLDHDG
                     RIRMDCSSPYAMASLVALKDRYDLAFGNDPDFDRHGIVTGKAGLMNPNHFLAAAVVHL
                     FSSRPSWLPSSGVGKTLVSSAMIDRAAELVRRELFETPVGFKWFVEPLIGGRVAFAGE
                     ESAGASLVCMDGSVWTTDKDGIAMALVAAEMLARTGKDPSDLYRELEERFGVYHYERI
                     DVPAGPREKAALSGITPDMVEARVLAGDAVTAVLTRAPANGAAIGGIKVVTASGWFAA
                     RPSGTEDVYKIYAESFRGPDHLALIQEEARSLVSTVFGRQASTGGGA"
     gene            2262..3086
                     /locus_tag="PLT69_01120"
     CDS             2262..3086
                     /locus_tag="PLT69_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ13082.1"
                     /translation="MRGPGTAARPPGTTAKRFGLAGDLAWYLAFVGVVALIVGKGIAS
                     SGYTWQWYRVPRFFVEFTAAGVVLGPLSRGLLVTFKITLASLVLAFAFGVAAALMRLS
                     GSVAARAAARVYLELVRNTPLIIQIFFMYFVVSPLVGLDSFLTAVLALSLFEGAYLCE
                     IFRAGILSVARGQWEAAQSLGLSAAETYRHVILPQAVRKIVPTVTGQTVSLVKDSALV
                     STIAVYDLAMQAQAIVAETFLVFEVWLAVAAVYLAVTMSLSAAAHAVEERFRRFGA"
     gene            3117..3947
                     /locus_tag="PLT69_01125"
     CDS             3117..3947
                     /locus_tag="PLT69_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ13083.1"
                     /translation="MMKRLTVVCTILAFGLGCALSPAYAKSLREDLAGQSTIEQVLKR
                     GVLRVGMSTFVPWAMNDKKGNLIGFEIDVAKRLAADMGVKVEFVPTKWSGIIPALLTG
                     KFDIIIGGMGITPERNLKVNFSRPYDYTGMSIVANKKLAKGFSRLEDFDKPSVAIAAR
                     TGTTAAAAVKKYLPKASLRLFDDESQAVQELLNGRVHALVASAPLPAFKALEYPDRLF
                     MPVKGDFTREPIGFAVVKGDVDTLNFLDNWITVVEAEGWFKERKHYWFETKDWKGLVE
                     "
     gene            3987..4853
                     /locus_tag="PLT69_01130"
     CDS             3987..4853
                     /locus_tag="PLT69_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ13084.1"
                     /translation="MGWIDVVVGAACAALVLWLGYLMGSRVRYNWNWSVIPQFFFYYD
                     EAGSRWVPNLIVKGFLMTIKLSVWATLLATPLGVLTALLRIGRNLVGRLLGRTYVELV
                     RNMPPLVLVYIVYYFVSDQLVRALGVETLLLSLGPGMQGFLEGCCAPVTKMPVFASAV
                     FMLAIYEGAYIAEIVRAGVQSIDRGQWEASSALGFTRLQQLRHVIFPQALARTLPPYA
                     GQVISTIKDSAIVSVISIPELTFQGLEIMASTYRTFEIWITITALYFVLTFTCSLGVQ
                     RLEAALGARRGY"
     gene            complement(5218..5877)
                     /locus_tag="PLT69_01135"
     CDS             complement(5218..5877)
                     /locus_tag="PLT69_01135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ13085.1"
                     /translation="MGSTGAFFDFDRTLIDVESPRLGILYLRRHRLIGPAFLLKVACA
                     NLLYKRGAISDHAMARILIGFYKGRQPAPFEEGAQGYWREVIRPHLAPAVVHRLREHQ
                     ASGHVTVLVSAGLRYLLTPVAAELGFDHLLCTELEKGPDGSFTGGTVGPICSGAVKGE
                     LVRRLAEREGIDLRASYAYGDHESDLAMLELVGHPVAVEPTQRLRVEALKRNWPIISH
                     R"
     gene            6235..7011
                     /gene="rph"
                     /locus_tag="PLT69_01140"
     CDS             6235..7011
                     /gene="rph"
                     /locus_tag="PLT69_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOJ13086.1"
                     /translation="MASIEELLRDKGLQPPKATRRGDNRAPDEMRRAVITRHYCKHAL
                     GSCLIEIGDTRVVCTASVENKVPPHLKNTGLGWITAEYGMLPASTSTRTAREAARGRP
                     AGRTHEIQRLIGRSLRAAASLADLGERTVWIDCDVLQADGGTRTAAITGAYVALHDAV
                     ACMLKDQLIARSPLIEQVAAVSVGIVNGQVLLDLTYEEDSNASVDMNVVMTRSGRIIE
                     VQGTAEKTPFTLDELMALIGMAKKGIGELIESQELALKGT"
     gene            7008..7625
                     /gene="rdgB"
                     /locus_tag="PLT69_01145"
     CDS             7008..7625
                     /gene="rdgB"
                     /locus_tag="PLT69_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOJ13087.1"
                     /translation="MKILAATSNPGKISEIRRILGAGGITVVTPGEMGIEIDVEENGD
                     TFEDNAVAKAVAYLRASGMPSLADDSGLCVEALGGRPGVRSARFAGQGASDRENYELL
                     LRLMEGVSDRRAAFVCTAALALEDGRIVTATGRCEGLILEAPRGASGFGYDPVFLEPA
                     SGKTFAELAEDEKNAISHRGRALVALMATLEEMGLLPGKDPASCA"
     gene            8228..8629
                     /locus_tag="PLT69_01150"
     CDS             8228..8629
                     /locus_tag="PLT69_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOJ13088.1"
                     /translation="MPYALVDHTADMALRVRAPSIEALFAEAALGLCEVLDARTSYPE
                     ETLRVELEGMDMEDLLVRWLQEVLYLVGVKGFRVYTVGRIAVAGTCLAAELEGAFGCG
                     TLALEVKAVTYHGLSIERDGGDVTATVVLDV"
     gene            8641..>9055
                     /locus_tag="PLT69_01155"
     CDS             8641..>9055
                     /locus_tag="PLT69_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOJ13089.1"
                     /translation="MPQPVKRRIDEYRIEIERVPPMRSRGLVYANRAIERTIEHDNVL
                     QQVVNVACLPGIIGPSIAMPDIHWGYGFPIGGVAAFSLDEGVISPGGVGYDINCGVGC
                     ILTGLEEKDAALHTEVLLAALFNEVPSGVGSKGGIT"
BASE COUNT         1482 a         2916 c         3125 g         1532 t
ORIGIN      
        1 cggccacctg gcccaggccc ttcgcctcgt cgaggaccgc ctgtatggcg gcgtgcatgc
       61 cctcgtaggc ccgtgcgacc ctctccctga atgcctcgct cacgtcgctc atctcctgca
      121 tgaggttgcc gatggggcag ccgcggctga accccatctt ctcgaacatg ttctcgtagg
      181 cgtccatgaa gcgagcgagc ctttccaggg ggggagtccc ctggtcgccg aggagtttct
      241 cgcccgccga ctcgatgagg ctctggaagt ggtccacgag ggcgagcccg aagtcctcct
      301 tgcttttgaa atggaagtag aacgatccct tgggcacgcc cgcggcatcc agtatctcct
      361 tgagcccggc cgccctgaaa ccccgggcgt gcacgatgcg cgcgccttct tcgatgatgg
      421 tctgcctcga gggggtgcgg gccatggggg atataataga ccggtcgtct acatccgtca
      481 accccgtttc tcgcccgagg tttaccggtg agcacaattc gggtacaatg gcggggtcgt
      541 tgccggatgt cccgcgttcg gacgtttttc gatccacgat tgacaggagg gtgagtcatg
      601 gccgtccatg acctggcagg gaagcccgtg ccaagatcga tgctcgtgga caccgcctcg
      661 ctggtggatg cgtactaccg caccaggccc gacccggccc acccggggtg ccgcgtgtcg
      721 ttcggcacct cgggacacag ggggtcgtcg atcgccgcca gcttcaacga ggaccacatc
      781 ctggccatca cgcaggccat ctgcgattac aagagggaat tcgggatcac gggccccgtg
      841 ttcgtgggca gggacaccca cgcgctgtcg gagccggcct tcaagaccgc gatcgaggtg
      901 cttgcagcca acgctctcac ggcgatggtg gacgaggacc tggggttcac ccccacgccg
      961 tcggtgtcca gggccgtcat cgagtacaac cgctcgaggt catcggaccc cgcggacggg
     1021 atcgtgatca ccccttcgca caacggcccc gaggacggcg ggttcaagta caacccgccg
     1081 gagggaggcc ccgcggacac caagaccacg gggtggatcg agaaacgcgc gaacgccctg
     1141 ttggagggcc gcctggccgg tgtcaggcgg atcccctacg aacgggcgct gtcagccccc
     1201 accaccacca ggtacgactt caccgggccg tatgtcgagg ggctcgcctc ggtcatcgac
     1261 atgggatcca tcgcatcttc cggcctcagg ctctgcgccg acgccctggg cggttcgggc
     1321 gcgtcctact ggcaccggat agccgagcgc tacgccctgt ccatagatgt cctgcacgac
     1381 accgcggatg cgaccttcgg cttcatgacc ctcgatcacg acggccggat ccgcatggac
     1441 tgttcgtcgc cgtacgccat ggcgtccctc gtggcgctga aagaccgcta cgacctcgcc
     1501 ttcggcaacg acccggactt cgacaggcac gggatcgtga ccgggaaggc cgggctcatg
     1561 aacccgaacc acttcctggc ggcggccgtg gtgcacctct tttcctcgag gccctcctgg
     1621 ctgccgtcct cgggcgtggg caagacgctc gtgagcagcg ccatgattga cagggccgcg
     1681 gagctcgtcc gaagggagct cttcgagacc ccggtagggt tcaagtggtt cgtcgagccc
     1741 ctgatcgggg gccgcgtggc cttcgcggga gaggagagcg caggcgcgtc gctcgtgtgc
     1801 atggacggct cggtgtggac cacggacaag gacggcatcg ccatggcgct cgtcgcggcg
     1861 gagatgctcg cgcgcacggg caaggacccc tcggatctct accgggaact cgaagaacgc
     1921 ttcggcgtgt accactacga gaggatcgac gtgcctgcgg gcccgaggga gaaggcggcg
     1981 ctctcgggaa tcaccccgga catggtcgag gcgagggtgc tggccggcga tgcggtgaca
     2041 gccgtcctca cgagggcgcc ggcgaacggc gcggccatcg gcggcataaa ggtcgtaacg
     2101 gcctcgggct ggttcgctgc tcggccctcg ggcacggagg acgtctacaa gatctacgca
     2161 gagagcttca ggggtcccga ccatctggcc ctcatccagg aagaggcgcg ctcgctcgta
     2221 agcaccgtct tcggccgcca ggcctccacg gggggtgggg cgtgagggga cccgggaccg
     2281 cggcccgacc acccggcaca accgccaaac ggttcgggct cgccggggac ctcgcctggt
     2341 acctcgcctt cgtaggggtc gtcgcgttga ttgtggggaa ggggattgcc tcgtcggggt
     2401 acacctggca gtggtaccgg gtcccgaggt tcttcgtgga gttcacggcg gcgggtgtcg
     2461 tgctcgggcc gctttcgagg ggactgctcg tgaccttcaa gatcaccctg gccagcctcg
     2521 tgctcgcctt cgccttcggg gtggcggccg ccctcatgag gctctcgggg tccgtggcgg
     2581 cccgggcggc cgcccgggtg tacctcgagc tcgtgaggaa cacgccgctc atcatccaga
     2641 tcttcttcat gtacttcgtc gtctctcccc tggtgggact cgactcgttc ctcaccgcgg
     2701 tgctggcgct cagcctgttc gagggggcgt acctgtgcga gatcttccgc gccgggatcc
     2761 tgtcggtcgc gaggggccag tgggaggcgg cgcagagcct cgggttgagc gcggccgaga
     2821 cgtaccgcca cgtgatcctg ccccaggccg tacgcaagat cgtccccacg gtgaccggcc
     2881 agaccgtgtc cctcgtgaag gactccgcgc tcgtgagcac catcgccgtg tacgacctcg
     2941 ccatgcaggc gcaggcgatc gtcgcggaga cctttctcgt gttcgaggtg tggcttgcgg
     3001 tggccgcggt ctacctggcc gtcacgatga gcctgtcggc cgcggcgcac gcagtggagg
     3061 agaggttcag gcggttcggg gcgtgacccc cgcatcggga atcagggagg tgaaccatga
     3121 tgaaacggtt gacggtcgta tgcacgatcc tggcgttcgg actcggatgc gctctgtcgc
     3181 ccgcctacgc gaagagcctc agggaggatc tcgcaggcca gagcaccatc gagcaggtgc
     3241 tgaagagggg ggtcctcagg gtgggcatgt ccaccttcgt cccctgggcc atgaacgaca
     3301 agaagggcaa cctcatcggc ttcgagatcg acgtggccaa gaggctcgcc gccgacatgg
     3361 gggtgaaggt cgagttcgtg cccaccaagt ggtcgggcat catccccgcc ctgctcacgg
     3421 gcaagttcga catcatcatc gggggcatgg gcatcacccc ggagcgtaac ctcaaggtga
     3481 acttctcgag accatacgac tacacgggca tgtccatcgt ggccaacaaa aagctcgcca
     3541 agggcttctc gaggctcgag gacttcgaca agccctcggt cgcaatcgcc gcccgcaccg
     3601 gcaccacggc ggccgcggcg gtgaagaagt acctgccgaa ggcctcgctc aggctcttcg
     3661 acgacgagtc ccaggccgtg caggagctct tgaacggccg cgtgcacgcg ctcgtcgcct
     3721 cggccccgct gccggccttc aaggcgctcg aataccccga caggctcttt atgccggtga
     3781 agggggactt caccagggag cccatcgggt tcgcggtggt caagggggac gtggacaccc
     3841 tgaacttcct ggacaattgg atcaccgtgg tggaggccga gggctggttc aaggagagga
     3901 agcactactg gttcgagacc aaagactgga aaggcctcgt ggagtgacgg accgcagggg
     3961 ggcgtctcga ggtgagcagg aggcccgtgg gttggatcga tgtggtcgtc ggggcggcgt
     4021 gtgccgcctt ggtgctgtgg ctcggctacc tcatgggatc cagggtcagg tacaactgga
     4081 actggtccgt gatcccccag ttcttcttct actacgacga ggccggctcg cgctgggttc
     4141 cgaacctcat cgtgaagggg ttcctcatga ccatcaagct cagcgtgtgg gcgaccctgc
     4201 tcgccacgcc cctgggcgtc ctgacggcgc ttctcaggat cggcaggaac ctggtcggga
     4261 gactcctggg caggacctac gtcgagctcg tcaggaacat gccgcccctt gtgctcgtct
     4321 acatcgtgta ctacttcgtg agcgaccagc tcgtccgggc gctcggcgtc gagacgctcc
     4381 tcttgtcgct cggtcccggc atgcagggat tcctcgaggg ctgctgcgcc ccggtgacca
     4441 agatgcccgt gttcgcctcg gcggtcttca tgctcgccat ctacgaggga gcatacatcg
     4501 ccgagatcgt ccgggcaggg gtccagtcca tcgacagggg ccagtgggag gcatcgagcg
     4561 ccctcgggtt cactaggctc cagcagctcc gccacgtgat cttcccccag gccctggcgc
     4621 gcacgctgcc cccctatgcc ggccaggtga tctccacgat caaggactcg gccatcgtct
     4681 cggtcatctc gatcccggag ctcaccttcc agggcctgga gatcatggcg tccacctacc
     4741 ggaccttcga gatctggatc acgatcaccg ccttgtactt cgtcctgacc ttcacctgct
     4801 cgctgggtgt gcagaggctc gaggcggcgc tcggcgcccg caggggctat tgatgccggg
     4861 cggggtgatg ggcggccccg gcaagcggac accctttgat ggggccgctt cgagtcggcc
     4921 ctcgcatccg gcggggcagg ggataggtcg gtgtgcccga gcctgcacga aagagacggg
     4981 ggcctcgagg gccccaggtg gggtcgcaag cggggtaccg aagaccgtgc tcgacccgtc
     5041 gggaaaagag agagcccgcg gccgcgcaaa gaaggctgag gccgccttgc tccccacgtc
     5101 cacctgcccc tgggtcttgg gcccagggtg tggttgtcga gagggggaag gcgggcccgg
     5161 tctccgctgc cttttcccgt gcaggccagg atacctgagg tgcccgccgt agacgcgtca
     5221 gcggtgggaa atgatcggcc agttcctctt cagggcctcg accctgagcc tttgggtcgg
     5281 ctccacggcg acggggtgtc ccacgagttc cagcatggcg aggtcggact cgtggtcgcc
     5341 gtatgcgtag gacgcacgca ggtcgatacc ctccctttcg gccaggcgcc tcacgagttc
     5401 gcccttgacc gctcccgaac agatagggcc gacggtcccg ccggtgaacg agccgtccgg
     5461 acccttttcg agctccgtgc agaggaggtg gtcgaagccc agctcggccg caaccggcgt
     5521 gagcaggtac ctgagccccg ccgagacgag gacggtcacg tgccccgaag cctggtgctc
     5581 cctcagcctg tgcactacgg cgggggcgag gtgcggcctg atcacctcgc gccagtagcc
     5641 ttgggccccc tcctcgaagg gcgcaggctg cctgcccttg tagaagccga tgaggatcct
     5701 cgccatggca tggtcgctga tcgcacccct cttgtacagg aggttcgcgc atgcgacctt
     5761 gaggaggaag gcgggcccga tcaggcggtg ccgcctgagg tagaggatgc cgagcctcgg
     5821 gctctcgacg tcgatgagcg tcctgtcgaa gtcgaagaat gcgcctgtgg atcccatggg
     5881 tgtttccctg tcacaacgta caggaaaccg gccgacctgt gcaacggcga cgagcggcgt
     5941 gaagtgctcg atgacgttcc agcgcctcgc ggcatggagg cctttccctc caggggcgct
     6001 cttttcggtg tcgcgaggtg aagctgggca tccagccccc acccggtcgt cttcgcgagg
     6061 cgcctccccc tctcaggggg ccccggtctc tctcgcgtcc gccgacttgg tccgggggct
     6121 cccctcgaaa tgaggcatcc ggcagcttga agcctgggta tgatcctgcg ccgaggttga
     6181 cttcatgggg catggcccgt agaatgcccc aaaggacgcg aagggggctt ggcgatggca
     6241 tccatagaag aacttctgcg cgacaagggg ttgcagccgc ccaaggcgac gaggaggggc
     6301 gacaacaggg ccccggacga gatgaggcgc gccgtcatca cgaggcatta ttgcaagcac
     6361 gccctgggct cgtgcctcat cgagataggc gacacgaggg tggtgtgcac cgcgagcgtg
     6421 gagaacaagg tgccacccca cctcaagaac acgggtctgg gatggatcac ggccgagtac
     6481 gggatgctcc ccgcctcgac gagcacgagg accgcccgcg aggccgcgcg cgggaggccc
     6541 gccgggagga cccacgagat ccagcggctc atcggccgct cgctcagggc tgccgcgtcc
     6601 cttgccgacc tcggcgagcg gaccgtgtgg atagactgcg acgtgctcca ggccgacggc
     6661 gggaccagga cagcggccat caccggcgcc tacgtggcgc tccacgacgc cgtggcgtgc
     6721 atgctgaaag accagctcat cgcgaggagc cccctcatcg agcaggtggc cgccgtgtcc
     6781 gtgggcatcg tgaacgggca ggtcctcctc gacctcacct acgaggagga ttcgaacgcg
     6841 agcgtcgaca tgaacgtggt catgacgagg tcgggaagga tcatcgaggt ccaggggact
     6901 gccgagaaga cgccgttcac cttggacgag ctcatggctc tcatcggtat ggcgaaaaag
     6961 gggatcggcg agctcatcga gagccaggag ctcgcgctca agggcacgtg aagatcctgg
     7021 ctgccacctc caacccggga aagatctcgg agatcaggcg tatcctgggg gccggcggca
     7081 tcaccgtggt gacccccggc gagatgggca tcgagatcga cgtggaggag aacggggaca
     7141 ccttcgagga caacgccgtg gcgaaggccg tggcctacct cagggcgagc ggcatgccca
     7201 gcctcgccga cgactcgggg ttgtgcgtgg aggccttggg cggcaggccc ggggtgagat
     7261 ccgcgcgttt cgcgggacag ggggcctccg acagggaaaa ctacgagctc ctgctcaggc
     7321 tcatggaggg cgtgagcgac aggagggcgg ccttcgtgtg cacggcggcc ctcgcgctcg
     7381 aagacggccg catcgtaacg gccacgggca ggtgcgaggg cctgatcctc gaagcgccca
     7441 ggggcgcctc gggcttcggg tacgacccgg tgttcctcga gcccgcaagc gggaagacct
     7501 tcgccgagct cgcggaagac gagaagaacg cgatcagcca ccggggaaga gcgctcgtcg
     7561 ccctcatggc gaccctcgag gagatgggcc tgcttccggg caaggatccc gcatcgtgtg
     7621 catagggtgg ccgggctcgg cgaaagcctg gtgagcctgg ggcggggagc gcaccgcggc
     7681 cgcccgctct cccgggtcca ccccttcctg ccgggcgggg cccgggttct cagcgaaccc
     7741 tgagactgga ccccgttcgc atgtgggtga atcggggggg cacaccagaa aaagaccacc
     7801 cctaggcctg gacatcgtgc gcacgagggt gaatccgccc tccagtaccg ggaagcgagc
     7861 gacgacaggg ctggaccccg tgcgcatgag ggtgaatctt ccccgcccgc aggtcgtgga
     7921 ggcgcgctgg actgggcctt gtgcgcatga gggtgaatct cgggcggact tttacggtcc
     7981 gtctgtgcaa tccgcatctt ccatggggag cgtccctgcg gctttgtgca acgagcgcaa
     8041 cgtgagggcg cggggcgacc ccgtcctttc aagcgctctc cccaggtttc gtgcaaccgc
     8101 aggcccgcct tcgaggggga tgcaccgcct gccgttcggg catgggaggt cgatcgcgtc
     8161 ttttgcctgc gggcatgctg gatgtgtaca agcagcccct gttgtgatag cattttcttt
     8221 tccggccatg ccgtacgctc tcgtggacca taccgcggat atggcgcttc gggtcagggc
     8281 cccgtctatc gaggccctct tcgccgaggc cgccctcggc ctctgcgagg tgctcgacgc
     8341 ccgtacctcg tatcccgagg agaccctgcg cgtggaactc gagggcatgg acatggagga
     8401 cctcctcgtg aggtggttgc aggaggtgct ctacctggtg ggggtgaagg ggttcagggt
     8461 gtacaccgtg ggccgcatcg ccgttgcggg aacttgcctg gcggcggagc tcgaaggcgc
     8521 gttcgggtgc gggactctcg ccctcgaggt gaaggccgtg acctaccacg ggctctcgat
     8581 cgagcgcgac gggggggacg tcacggccac cgtcgttctc gacgtgtaat ggggggatcc
     8641 atgcctcaac cggtcaaacg ccgcatcgac gagtaccgca tcgagatcga gcgtgtcccg
     8701 cccatgagga gcagggggct cgtgtacgcc aacagggcga tcgagcgcac gatcgagcac
     8761 gacaacgtgc tgcagcaggt ggtgaacgtg gcgtgcctgc cgggcatcat cgggccctcg
     8821 atcgccatgc ccgacatcca ctggggctac gggttcccca tcggcggggt ggccgccttc
     8881 tcccttgacg agggggtcat ctccccggga ggggtgggct acgacatcaa ttgcggtgtg
     8941 gggtgcatcc tcaccgggct cgaggagaag gacgccgcct tgcatacgga ggtcctgctg
     9001 gcggcgctct tcaacgaggt cccctcggga gtggggtcca aaggcggcat caccg
//
LOCUS       DAOSCR010000020         8921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5130_length_8921_cov_3.687119, whole genome shotgun sequence.
ACCESSION   DAOSCR010000020 DAOSCR010000000
VERSION     DAOSCR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_8921_cov_3.687119"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLT69_01160"
     CDS             complement(<1..901)
                     /locus_tag="PLT69_01160"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ13090.1"
                     /translation="MSTSQDRIIIFDTTLRDGEQSPGASMDVHEKVLVAKQLEMLGVD
                     KIEAGFPISSPGDFDAVQAIAGEITKSSVVGLCRAKDEDIKAAWEAVKDARKPCIHTF
                     IATSDIHLKYQLRMSREEVLKAVEHSVGLCSSLCNDVEFSAMDATRSDWDYLADVYTV
                     AIEAGAKTINVPDTVGYTNPQEFYNLITHLRNKVRGIEKAVISVHCHNDLGMAVANSV
                     SAVMAGARQIECTINGIGERAGNTSLEEVVMIFNVRKDLYGVVPAIDTTLLYPTSRLV
                     SHITGIPVQPNKAVVGANAFAHES"
     gene            complement(867..1682)
                     /gene="pssA"
                     /locus_tag="PLT69_01165"
     CDS             complement(867..1682)
                     /gene="pssA"
                     /locus_tag="PLT69_01165"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOJ13091.1"
                     /translation="MRRFRPHGHKQEHNKPRVPFLPNFITTMSLFCGFYSIMFSLKGD
                     FRWASILILISGFIDGLDGRVARMTGTTSEFGKEYDSLSDVIAFGVAPGLMVYLWQLN
                     AFGRLGFVAAFLYVACGALRLARFNVDTSGSLTHFTGLPIPMAAATVTSTYLFTEELA
                     KVVGPMPGTVSLAILLGVYIVSFLMVSSVPYPSFKHVSYIKAHPFQLLVAGVLLLVVV
                     ALAPAVMLFVLTMTFVVGGALWSAGAYAVQVGRQKKGLKGGSHEHIPGQDHHI"
     gene            complement(1679..2302)
                     /locus_tag="PLT69_01170"
     CDS             complement(1679..2302)
                     /locus_tag="PLT69_01170"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOJ13092.1"
                     /translation="MRIRREGLPFVAGAAAVLALALVFGGRAARLLAGLPVLFVTWFF
                     RDPERVVPKGEGIVVSPADGEVIEVAETEEEIVGPCTKVAVFMNVFSVHVNRLPVSGR
                     VRETRYRPGTFEVASLGKKTEANERMMVYIDTDHGTFRVDQVAGLVARRISCWLSPGD
                     KAVMGERFGLIRFGSLLECWMPRGYKAVCERGQKVRAGESVIGRRSA"
     gene            complement(2345..3361)
                     /gene="ilvC"
                     /locus_tag="PLT69_01175"
     CDS             complement(2345..3361)
                     /gene="ilvC"
                     /locus_tag="PLT69_01175"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOJ13093.1"
                     /translation="MKVFYDKDADIKLIRSKKVAVIGYGSQGFAHSNNLKDSGVDVVV
                     GLRKGSPSAAKAKKAGLKVMTPSEAAKWADVIMMLIPDELQAKVYSSEIAAHMSKGKY
                     LVFAHGFNIHYGQIVPPDGVNCFMVAPKSPGHMVRYEFTQGRGVPMLIAVHADPSGDT
                     RALALSYAKAIGGGRAGILETTFKEETETDLFGEQVVLCGGTTALIQAGFETLVEAGY
                     SPEMAYFECLHELKLIVDLIYEGGISNMRYSISNTAQYGDLTRGPVIIDGAVRERMRK
                     ILADIQDGTFAKEWVLENMAGRPRFNALTRMGEEHPIEKVGEKLRAMMPWIAKEKKVD
                     KKLN"
     gene            complement(3358..3864)
                     /gene="ilvN"
                     /locus_tag="PLT69_01180"
     CDS             complement(3358..3864)
                     /gene="ilvN"
                     /locus_tag="PLT69_01180"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOJ13094.1"
                     /translation="MNERHAISILVDNQAGVLSRVVGLFSGRGFNIESLSVAPTLDPT
                     KSKMVITTTGNEQVLEQIKKQLNKLINVIKVVDFVDKDYVEREMILVKVKAKEQTRAE
                     VLRISDIFRARVVDVSPVSYTLALTGDQGKLRAFIELLTPMGIIDFVRTGTIVMEREM
                     QSRKRSDQ"
     gene            complement(3872..5569)
                     /gene="ilvB"
                     /locus_tag="PLT69_01185"
     CDS             complement(3872..5569)
                     /gene="ilvB"
                     /locus_tag="PLT69_01185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOJ13095.1"
                     /translation="MKKITGAQAFIEALRCEGVKHVFGYPGGAVLDIFDVIMDAHDIM
                     FVLTRHEQGAVHAADGYARASGKVGVALVTSGPGATNTVTGIATAFMDSIPVVVFSGQ
                     VSTSLIGNDAFQEADTVGITRPCTKHNYLVKDVEDLPRIIKEAFYIASTGRPGPVLVD
                     IPKDVMRGLVQFRYPEKVHLEGYCPTYRGHRGQIKRILSTILKSRHPVIYAGGGVILS
                     NASAELTRLARLLNIPVTTTLMGLGGFPADDPLWLGMLGMHGTYAANMAVTETDCLIA
                     IGARFDDRVTGKVDEFAPRARIIHIDIDPTSISKNVQVHLPVVGDCGNVMRDMLEELS
                     GMEREIEDLKRTVRPWIERLEEWKRDKPLRYDKGTGIKPQYVIEKIRELAPEDAIIST
                     EVGQNQMWTAQYYKFNAPRLFLTSGGLGTMGYGFPAAIGAQVAFPGRTVIDIAGDGSI
                     QMNIQELATAVQYRLPVKVMIINNGYLGMVRQWQSLFYKGRYSHTSMAVSPDFVKLAE
                     AYGAHGIRVEDPGDVEDAVRKALSMEGCVFVDFKVEPEEDVYPMVPAGAPINNMLLV"
     gene            complement(5666..5893)
                     /locus_tag="PLT69_01190"
     CDS             complement(5666..5893)
                     /locus_tag="PLT69_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOJ13096.1"
                     /translation="MEEADLQLIRKLIPRDEELKRLWDEHLEYEKKLEALGKRRYLST
                     EEDMKRKELQKLKLKGKDRIEEILRRYRSGK"
     gene            complement(6128..6544)
                     /locus_tag="PLT69_01195"
     CDS             complement(6128..6544)
                     /locus_tag="PLT69_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13097.1"
                     /translation="MGVASFLGWCTAAAVLSTLMNFVLKQVSRDYVKAISARRPSFAS
                     AYRNLVRLVVRTHPVFGFAALAAALAHVSYALLHGIVSLTGAATLVLLCACVSLGAFG
                     RYVVQRPRGWWIHLHRAAALLASLGLVLHVPLKAFV"
     gene            7111..8328
                     /locus_tag="PLT69_01200"
     CDS             7111..8328
                     /locus_tag="PLT69_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ13098.1"
                     /translation="MDIYIARQPIFSRTRKVVGYELLFRGGMENYFPGIDGDTATSRV
                     LSGTFFTTGIDRIAAGRQAYINFPRNLVLRKIPLMFPPETTTVEVLENVNADAEVVES
                     LREIASKGYTVALDDFSYRDGVGPLVDLCHIVKVDFVHSTPEEIRTIAGDMLSRGKRV
                     LAEKVETHADFERAIGLGFSLFQGYFFSKPEIIKDTDIPALKMNLLRIMGEAQQSDYD
                     VGRLKSLIEQDVGVSYKLLRYLNSPFFRRVSPITSIRQAIVMLGEKGIRAFISIILLA
                     ELGQDKPDELIKSSILRARMCELAGKKGGSRVDPSELFMMGLFSHIDAILDSDMKSVL
                     ERLPLAGEIKDALGDRTGPLAPYIELVEGYERGLWEESGRTSNELGIDEEDLPAIYMD
                     ALTYADALFDSSQ"
     gene            complement(8362..>8921)
                     /locus_tag="PLT69_01205"
     CDS             complement(8362..>8921)
                     /locus_tag="PLT69_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ13099.1"
                     /translation="YAFEYTRRRNRRKTLALVGKTNVLTYVFDLWERAFHEIGSKDYP
                     DITREYYHVDATCMWMVKNPEWFDVIVTENMFGDIITDLGAITQGGMGIAAGGNINPE
                     GVSMFEPIGGSAPKYTGMNVINPLAAIAAAQMMIETLSENRAAARIEKGIVKALERDI
                     QSLAAGKMGLGTREVGDKVAAYAAE"
BASE COUNT         1541 a         2957 c         2708 g         1715 t
ORIGIN      
        1 ccgactcgtg ggcgaaggcg ttcgcgccca ccacggcctt gttcggctgc acgggtatgc
       61 ccgtgatgtg ggaaacgagc ctgctcgtcg ggtagagcag ggtggtgtcg atggcgggca
      121 cgacgccgta gaggtccttg cggacgttga agatcatgac cacctcctcc agggaggtgt
      181 tgccggcgcg ctcgccgatg ccgtttatgg tgcactcgat ctgcctcgct cccgccatca
      241 ccgcggagac cgagttggcc accgccatgc cgaggtcgtt atggcagtgc acgctgatca
      301 cggccttctc tatccccctc accttgttcc tcaggtgggt gatgaggttg tagaactcct
      361 gggggttcgt gtaccccacc gtgtcgggca cgttgatcgt cttggcgccg gcctcgatgg
      421 ccacggtgta gacgtccgcg aggtagtccc agtcggatct cgtggcgtcc atggccgaga
      481 actcgacgtc gttgcacaac gaggaacaca ggccgaccga gtgctccacg gccttgagca
      541 cttcctccct ggacatccgg agctggtact tgaggtggat gtccgaggtg gctatgaagg
      601 tgtggatgca cggcttcctc gcatccttga ccgcttccca tgcggccttg atgtcctcgt
      661 ccttcgccct gcacaggccg acgaccgacg acttggtgat ctccccggca atcgcctgga
      721 ccgcgtcgaa gtccccgggc gaggatatgg ggaaaccggc ctctatcttg tccacgccga
      781 gcatctcgag ctgcttggcg accagcacct tctcgtggac gtccatgctc gcgccgggcg
      841 actgttcccc gtccctgagc gtcgtgtcaa atatgatgat cctgtcctgg gatgtgctca
      901 tgtgatccac ccttgagtcc tttcttctgc cttccgacct gcaccgcata cgcgcctgcg
      961 ctccacagcg ccccgccgac gacgaaggtc atcgtcagca cgaagagcat gaccgcgggc
     1021 gcaagcgcaa ccaccacgag cagcaggacc cccgcgacaa gcagctggaa cgggtgcgcc
     1081 ttgatgtagc tcacgtgctt gaagctcggg taggggacgc tgctcaccat gaggaacgaa
     1141 acgatgtaca cgccgaggag gatcgccagc gagacggtgc cgggcatggg acccaccacc
     1201 ttggcgagtt cctcggtgaa gaggtaggtg ctcgtcaccg tggccgccgc catggggatc
     1261 ggcagcccgg tgaagtgcgt gagggaaccc gaggtgtcga cgttgaagcg cgccagcctg
     1321 agcgccccgc aggccacgta caggaaggcg gcgacgaagc cgagcctgcc gaaggcgttg
     1381 agctgccaga ggtacaccat gagccccggg gccaccccga aggcgatcac gtcggagagg
     1441 gagtcgtact ccttcccgaa ctccgaggtg gttcccgtca tgcgcgcgac cctcccgtcc
     1501 agcccgtcga tgaagccgga tatcaggatg aggatcgagg cccagcggaa gtcccccttg
     1561 agggagaaca tgatggagta gaacccgcag aacaggctca tcgtggtgat gaagttcggg
     1621 agaaacggca ccctcggctt gttgtgctcc tgcttgtggc cgtgcggccg gaaacgcctc
     1681 atgcagacct ccttccaatc acgctctcgc ccgctctcac cttctgtccc cgctcgcaga
     1741 ccgccttgta tcccctcggc atccagcact ccaagaggga cccgaacctg atgagcccga
     1801 accgctctcc catgacggcc ttgtcacccg gggagagcca gcacgatatc ctcctcgcca
     1861 cgagccccgc cacctggtcc acgcggaacg tgccgtggtc cgtgtcgatg tacaccatca
     1921 tgcgctcgtt cgcctcggtc ttcttcccga ggctcgcgac ctcgaaggtg cccggcctgt
     1981 accgcgtctc cctcaccctc ccggaaacgg gcagcctgtt gacgtggaca ctgaacacgt
     2041 tcatgaagac cgccaccttc gtgcacgggc ccacgatctc ttcctcggtc tcggccacct
     2101 cgatcacctc cccgtcggcg ggggagacca cgatcccttc ccccttcggc accacgcgct
     2161 cggggtccct gaagaaccac gtgacgaaga ggaccggcag gccggccagc agccttgcgg
     2221 ccctgccccc gaagacgagg gcaagggcga gcaccgctgc agcgcccgca acgaacggca
     2281 acccttccct cctgatgcgc atctataccc tgtccttccg gccgcacgac atcctggagc
     2341 ggccctagtt cagcttctta tcaaccttct tctccttggc gatccacggc atcatcgccc
     2401 tcagcttctc gcccaccttc tcgatggggt gctcctcgcc catcctcgtc agcgcattga
     2461 agcggggcct gccggccatg ttctcgagca cccactcctt ggcgaacgtg ccgtcctgga
     2521 tgtcggccag gatcttgcgc atgcgttccc tcaccgcgcc gtcgatgatc acgggcccgc
     2581 gggtgaggtc gccgtactgc gcggtgttgc tgatggaata gcgcatgttg gagatcccgc
     2641 cctcgtagat gaggtccacg atgagcttga gctcgtgcag gcactcgaag tacgccatct
     2701 ccggcgagta gccggcctcg acgagcgtct cgaagcccgc ctggatcagg gcggtcgtgc
     2761 cgccgcagag gaccacctgc tcaccgaaga ggtccgtctc ggtctcctcc ttgaaggtgg
     2821 tctccaggat cccggccctt ccacccccga tggccttggc gtacgagagc gcgagagcgc
     2881 gcgtgtcacc cgaagggtca gcgtgcacgg cgatgagcat gggcacgccc ctgccctgcg
     2941 tgaactcgta cctcaccatg tggccgggtg acttgggggc gaccatgaag cagttcacgc
     3001 cgtcgggcgg cacgatctgc ccgtagtgga tgttgaagcc gtgggcgaag acgaggtact
     3061 tgcccttgct catgtgggcg gcgatctcgc tcgagtagac cttcgcctgc agctcgtcgg
     3121 ggatgagcat catgatcacg tcagcccact tcgccgcctc ggacggcgtc atgaccttga
     3181 ggcccgcctt cttggccttg gccgccgagg ggctgccctt gcgaagaccc accaccacgt
     3241 cgacccccga gtctttcagg ttgttcgagt gggcgaagcc ctgggacccg tagcctatga
     3301 ccgccacctt cttggacctg atgagcttga tgtccgcatc cttgtcgtag aataccttca
     3361 ttgatcgctc cttttcctgc tctgcatctc cctttccatg acgatggtcc ccgtccggac
     3421 gaagtcgatg atgcccatgg gcgtgagaag ctcgatgaaa gccctgagct ttccctggtc
     3481 ccccgtgagc gccagggtgt acgacaccgg cgacacgtcc accacccggg ccctgaatat
     3541 gtcggatatc ctgaggacct cggccctggt ctgctccttc gccttgacct tgacgaggat
     3601 catctccctc tccacgtaat ccttgtcgac gaaatccacc accttgatca cgttgatgag
     3661 cttgttgagc tgcttcttta tctgctcgag cacctgctcg ttgccggtgg tggtgatgac
     3721 catcttggat ttcgtggggt cgagcgtggg ggcgaccgag agggactcga tgttgaaccc
     3781 cctgccggaa aacagcccca cgacgcgaga gagaaccccc gcctggttgt ccacgagtat
     3841 ggaaatggcg tgtctttcgt tcatcgccgc ctcacaccaa gagcatgttg ttgatgggcg
     3901 cgcctgcggg caccatggga tacacgtcct cctccggctc caccttgaaa tccacgaaca
     3961 cgcacccctc catggacaag gctttcctga ccgcgtcttc cacgtccccc gggtcctcca
     4021 cccgtatccc gtgcgcgccg taggcctcgg cgagcttcac gaagtcgggc gagacggcca
     4081 tggacgtgtg ggaatacctg cccttgtaga agagtgactg ccactgcctc accatgccga
     4141 ggtacccgtt gttgatgatc atgaccttga cggggagcct gtactggacg gcggtcgcga
     4201 gctcctggat gttcatctgg atcgagccgt ctccggcgat gtctatgacc gtacggccgg
     4261 ggaaggcgac ctgcgccccg atggccgcag ggaacccgta gcccatggtc ccgaggccgc
     4321 cggaagtgag gaacagcctc ggcgcgttga acttgtagta ctgcgccgtc cacatctggt
     4381 tctggcccac ctcggtggag atgatggcgt cttcgggcgc cagttcgcgg atcttctcga
     4441 tcacgtactg gggcttgatg cccgttccct tgtcatacct gaggggcttg tccctcttcc
     4501 attcctcgag gcgctctatc cacggcctca ccgtgcgctt caggtcttct atctcgcgct
     4561 ccatgcccga gagttcctcg agcatgtcgc gcatgacgtt gccgcagtct cccaccacgg
     4621 ggaggtggac ctggacgttc ttgctgatgg aggtcgggtc gatgtcgatg tgtatgatgc
     4681 gggccctggg cgcgaactcg tccaccttgc cggtcaccct gtcgtcgaac cgggcgccga
     4741 tggctatgag acagtccgtc tcggtcacgg ccatgttggc ggcgtaggtg ccgtgcatgc
     4801 cgagcatgcc cagccacagc gggtcgtcgg ccgggaaccc cccgaggccc atgagggtgg
     4861 tcgtgaccgg tatgttgagg agccgcgcga gcctcgtgag ctccgcggac gcgttggaga
     4921 ggatcacccc gccgcccgcg tagatcaccg ggtgtctgct cttgagtatg gtggagagga
     4981 tgcgcttgat ctgccccctg tgcccccggt acgtggggca gtacccctcc aggtgcacct
     5041 tctccgggta cctgaactgg acgagccccc gcatcacgtc cttgggtatg tccacgagga
     5101 ccggccccgg cctgcccgtg gaggcgatgt agaacgcctc cttgatgatg cgcggcaggt
     5161 cttccacgtc cttgaccagg tagttgtgct tggtgcatgg tctcgtgatg ccgaccgtgt
     5221 ccgcctcctg gaaggcgtcg ttgccgatga ggctcgtcga gacctggccg gagaacacga
     5281 ccacggggat cgagtccatg aaggccgtgg ctatgcccgt cacggtgttc gtcgcccccg
     5341 gcccggaggt gacgagcgcg accccgacct ttccgctcgc ccgcgcgtac ccgtccgccg
     5401 catggaccgc cccctgctcg tggcgtgtca gcacgaacat gatgtcgtgc gcgtccatga
     5461 tgacatcgaa gatgtccagg acagccccgc cggggtaacc gaacacgtgc ttgacgccct
     5521 cgcatctgag cgcctctatg aacgcctgtg cgcctgttat cttcttcatt ggcgtgtttc
     5581 ctgaaacctc tctatatcta tagcatcacg ggtattcccc gccacatcgc cgcgtctcgg
     5641 gccaactcct tctaggcgac gggtctcact tcccgctcct gtacctgcga aggatctcct
     5701 cgatcctgtc cttgcccttg agcttgagct tctggagctc cttgcgcttc atgtcctcct
     5761 cggtgctcag gtaccttctc ttgccgagcg cctcgagctt cttctcgtac tcgaggtgtt
     5821 cgtcccagag tctcttgagt tcctcgtccc tgggaatgag cttgcgaatg agctggagat
     5881 cggcctcttc catatgctcc cccctctccg aacgataagg ttcatgtaga ccacatacaa
     5941 gtcactctgt caacagaggc ttccgacaac cgcgcacccg gcgagggcct cctggggcat
     6001 ccggcgtccg cctgcaggaa ggcatgcaga ggcggggcgg ggacacgaaa gtgcctcccc
     6061 cgggcgcggc cctggcacgc cgtgcgcgat gaaaccattc acgagacgga ccctcccccg
     6121 gcccggtcta cacgaacgcc ttgaggggca catggagcac caagcccagg gacgcgagca
     6181 gggcggctgc ccggtggagg tgaatccacc accccctcgg ccgctggacc acgtaacgac
     6241 cgaaggcgcc gagcgagacg catgcgcaca gcaggacgag cgtggccgca cccgtcaggg
     6301 acacgatccc gtgcaacagg gcgtacgata cgtgggcgag agccgctgca agggccgcga
     6361 acccgaaaac ggggtgcgtg cgcaccacga ggcggaccag gttccgatac gcggacgcga
     6421 acgagggcct tcttgcggat atcgccttga cgtagtcccg gctcacctgc ttcagcacga
     6481 agttcatgag tgtcgacagg acggccgcgg ccgtgcacca cccgaggaac gaagccaccc
     6541 ccatgcgtca caggcctccc atacccgcag attagacagc ggccgtcttc ccgtcatgtt
     6601 cctcttccgg tgtcatcatg cacacgatcg tggggcaggg tgcatggagc gttctggcac
     6661 gaaggcctcc ccaaggtctc cacccgtgca aggggtacat gaccggcccg ggggacgccg
     6721 tctttgcctt acgaaacatg ggagggggcg ggtatgggaa ggatgccgcg tggacgacac
     6781 acagggacga ggcatatgcg tcaaccgatc gccgtgaaca gtgcaggcac gcatcatcgc
     6841 gtaagacccc cttcccacgt ccatcccccc cttcagggag cgagcgtacc cgtcgaggcc
     6901 ccaaggcggt ccttgccgcc gtgcggtctc atcccggcct tgagcaccgg gcacgcaggc
     6961 gccgacaccc aggccccctg ccacgccggg agcatgtctc ctggccgatg gcgacgcgcg
     7021 atcggccggc ctgtcatgaa aaaacccgag agtacaaggc gatatgcggt gccgtaatgc
     7081 cgcgagactc gtcggccgat acatgttttc gtggatattt atattgcacg tcagcccatc
     7141 ttttcgcgga ccaggaaggt ggtcggctac gagctcctgt tcaggggggg catggaaaac
     7201 tacttccccg gcatagacgg tgacacggcc acttcgcgcg tcctgtccgg caccttcttc
     7261 accacgggca tagacaggat agcggcgggc cgccaggcgt acatcaactt tcccaggaac
     7321 ctggtgctga gaaagatccc tctcatgttc ccgcccgaga ccaccaccgt ggaggtcctc
     7381 gagaacgtga acgccgatgc cgaggtcgtg gagagcctga gggagatcgc ctcgaagggg
     7441 tacacggtgg ccctcgacga cttctcgtac cgagacggcg tggggccgct cgtcgatctc
     7501 tgccacatcg tcaaggtgga tttcgtgcac tcgacccccg aagagatacg caccattgcc
     7561 ggcgacatgc tgtccagggg caagcgggtg ctggccgaga aggtggagac gcacgccgac
     7621 ttcgaaagag ccatcggcct cgggttctcg ctcttccagg gctacttctt cagcaagcca
     7681 gagatcatca aggacaccga catccctgcc ctcaagatga acctgctgag gatcatgggc
     7741 gaagcccagc agagcgatta cgatgtcggc aggctgaagt cactcataga acaggacgtg
     7801 ggcgtgtcgt acaagctcct gaggtacctc aactccccgt tcttcaggag ggtctcgccc
     7861 atcacctcca tacgccaggc gatcgtcatg ctcggggaga agggcatacg ggcctttatc
     7921 tccatcatcc tccttgccga actggggcag gacaaacccg acgaactcat caagagctcg
     7981 atcttgaggg cgcggatgtg cgagctcgca ggcaagaagg gtggatcacg ggtggacccc
     8041 tccgagctct tcatgatggg cctgttctcc cacatcgacg ccatcctgga ctcggacatg
     8101 aagagcgtgc tcgaacgcct cccgctggcg ggcgagatca aggatgcgct gggggaccgt
     8161 acaggcccgc tcgcacccta catcgagctc gtggaggggt acgagcgggg tttgtgggaa
     8221 gagtccggac ggacttcgaa cgagctgggc atcgacgagg aggatctccc ggccatctac
     8281 atggacgccc tcacgtatgc ggacgccctc ttcgactcat cccagtgaca caaggcgggc
     8341 gggtcacccc gaccccgagg ctcactcggc tgcgtaggcg gcgaccttgt cccccacctc
     8401 tctcgtcccg agccccatct tcccggcggc cagggactgt atgtccctct cgagggcctt
     8461 cacgataccc ttctcgatgc gcgcggcagc ccggttttcg ctgagggtct cgatcatcat
     8521 ctgggcggct gcgatggcgg cgagggggtt gatgacgttc atcccggtgt acttgggtgc
     8581 ggaaccgcct atgggctcga acatcgagac gccctcaggg ttgatattgc cgcccgccgc
     8641 tatgcccatg ccgccctggg tgatggcgcc caggtcggtg atgatgtctc cgaacatgtt
     8701 ctccgtgacg atcacgtcga accactccgg gttcttgacc atccacatgc acgtggcgtc
     8761 cacgtggtag tactccctcg tgatgtcggg atagtccttg ctcccgatct cgtggaaggc
     8821 gcgctcccag agatcgaaga cgtaggtgag cacgttggtc ttccccacga gggcgagggt
     8881 cttcctcctg ttccgccttc gcgtgtactc gaacgcgtag c
//
LOCUS       DAOSCR010000021         8899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5140_length_8899_cov_3.435210, whole genome shotgun sequence.
ACCESSION   DAOSCR010000021 DAOSCR010000000
VERSION     DAOSCR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_8899_cov_3.435210"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2113)
                     /locus_tag="PLT69_01210"
     CDS             complement(113..2113)
                     /locus_tag="PLT69_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOJ13100.1"
                     /translation="MERWSRFFQSWQKDLVLWVFCMAFFACFRIAFLVVFRHRMEPTS
                     TFATILSAFLNGMRYDAKVSTTFMLVPFCFSTACIFKNVRVLAGRVRAATGLVFVCLS
                     SALCLITIEYFREYNDQFNHYLFGLIYDDRAAIFKTILSTYHASTHLVVFIVTVTLAG
                     ILLRRVLQTPLLEQGERDRIASRPALRRTVTVLIVAAIVFGLRGSIGAMPARERHAGI
                     TTDDFLNKTVLNPYSSLRYAIQRHIKISREKGVREYLPDGDVAKALRRVYGPRAVPGD
                     IDASLLRTAKGASARPPAHIFWIVAESYSAWPLEEKYASLHVADGLKELAHEGISCRP
                     FVASTQGTMSSLNTIVTGLPDADIQTNYRMSSKRPYPTSITTIFERLGYRTRAFYGGY
                     LTWQRVGDFLKDQGFDEVYGGGEMGSWLSANEWGVDDEHIFSFVLKTVKDEVPSFNLI
                     LTTSLHPPYTVDLPSKGFHRERLPSSIDGIPIDDDDARFLGHIWYADRCILLFCKEAS
                     SRYEESLFIVTADHPGRRWLAENPDPLERHLIPFVMYGRCLPRDTPHVIHTPGCHIDV
                     VPTVVELTAPKGFAYHTMGKDLLDSAYPAYAVGRDLFLTREALVDLRKAPRVIPLEGV
                     GPAVPLPFDVKDMQLLHDGIHGIAWWRVMKGPRTFEASPRPE"
     gene            complement(2425..2751)
                     /locus_tag="PLT69_01215"
     CDS             complement(2425..2751)
                     /locus_tag="PLT69_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13101.1"
                     /translation="MGKKEERLRTTWISFGAVILACTILAFSFAQAQQAPATKDTMTT
                     VMRSCSACHDTARVCRALGTRDAAGWERTIKRMVAKGASLGTEDIQAAAGLLATLAPG
                     SKPVCK"
     gene            complement(2939..4585)
                     /locus_tag="PLT69_01220"
     CDS             complement(2939..4585)
                     /locus_tag="PLT69_01220"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HOJ13102.1"
                     /translation="MAQRPTLKAEIARVLACTPAQRARGIIESPYTRQILEQLPPQEA
                     YLIMKESWGMDSQILLQYVPPETVCRFIDLDCWQRDSFSVDSAMEWLQELSLSSPETF
                     LQAVEALDMEILVLIFQQCFEVVHTRPTDENIQDLIDEGFESIDNVYFYRITGDEDHA
                     QFLKEVLSTLFTQRHDLYVTLLEAIMYELKSSLEEASYEKRTLRLVEMGFPSPEESVE
                     IYRRASIDRLLSLGIHRDKTPLLTRHLHMLPAEYLESFPGGTSLLAKALGRASDATRD
                     RFVYEMVYLSNKILMADYKPLNDSDELRAGMDKAGCLCTLGLKVAMREKGLPAHVILE
                     TVNAETLFSIGYNEALQLQHRLKRILDSVERSMIPTRMNGIVEGLLRKRPLYKDREFA
                     SPEDIDEVTGVLDTLEAMASVSDTLEWERAIPSLGGTNTGTALDLETVILTAAALNAV
                     TGETGFRPLTKDEFLAFVASATHVRGGKRTVKNRFERDLGVYLASLLLDAERRIRELV
                     TRALCLRIEDELGGILDTRTLDSRFVSCVTVRLEGASSRP"
     gene            complement(4672..5967)
                     /locus_tag="PLT69_01225"
     CDS             complement(4672..5967)
                     /locus_tag="PLT69_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ13103.1"
                     /translation="MRNTIRSAIALAVSVLIMAPVDCKAGSMTLEDALRVALENNPQI
                     KAQDQEVSIKEMEERSQLARMLPSADVSYGYVRLNEAPSITVPLAEPLTIPMGTKDNY
                     ELSLQAKQVLFAGGALYNAYLVSKNERHASMIERERASRQLKLQVIDAYYGVIKARQM
                     LEVAKSSMASVKSLLDMSQAFFDQGMIPKNNLLEAQVRYAETEQGLITAENAVRIAET
                     GFNLLLARAPSEEVSIAEEIPVAKVDMTLEQAIEQAFENRQEIRVAKTQLDSAHKGVT
                     IARSRFMPNVAATYTYTRSGEDPDVEEDSWKVGLGLSWNLFEGGAGVWGYNKASYTRA
                     KAEYLLESLKNMVTLEVKSAYLNTLEAQARLKVAERAIDQAQENFRIEKERYSFQVST
                     STDVLRAETLLAQARNNLISARADQAKAMASLLASMGTL"
     gene            complement(6307..7590)
                     /locus_tag="PLT69_01230"
     CDS             complement(6307..7590)
                     /locus_tag="PLT69_01230"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOJ13104.1"
                     /translation="MPKIRSFRGIRYNPERIGLDSVICPPYDVITNQQLEELYARSPY
                     NAVRLVLGKQHPKDDKDNNRYTRARDYFHQWLSEGVLIEDEKPAIYYHEHTFTLGDKT
                     YTRKGFIASVRLDDDDKKVIRPHEHTVKSPKLDRLRLMNEVRMNFSSVLGLYADSKKI
                     IETQVKPRLGDPHVEFTFRDETHRLWVIDETALVDKVSRLMQNKKIVLADGQHRYETA
                     KIYRDRMRAATGKSDGNQNFDYIQMYLVNVDEGVKILPMHRVILDSMGVGLVDLEYRI
                     KEIYNMIPYDNRKGFISALNKAGKGAVGLFVKGIPRFYLLQLAHEEDLERMMPQHIPH
                     QLRKNDVTILHDCVIEPVLGINANIENRRISYTSKAEEALDMVSKGQADIAFLLNPPS
                     IEEVFEIAEAGLRMPHNSTYFYPKVPTGLVFHSIG"
     gene            7842..8204
                     /locus_tag="PLT69_01235"
     CDS             7842..8204
                     /locus_tag="PLT69_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13105.1"
                     /translation="MASGIAIQYLKGYTWCMRYAGLALSMGVLLAFIVFLVNNSQEFR
                     LGFMGYELEAPLALWILMLVFFAAGTLPVLATEVFRSVRTRVRRRVLEAEIRSLEEEL
                     SSGSGPAKDSREPSPDKP"
     gene            complement(8157..8810)
                     /locus_tag="PLT69_01240"
     CDS             complement(8157..8810)
                     /locus_tag="PLT69_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13106.1"
                     /translation="MASPNHSEGEIFLKRRRIYGPDVRLIEKDGILLVEKTYRDRPLP
                     VRVAGRLLIRWETFVYSKLSGIPGIPVLATSPDRYTITTTFMGGGNLRARARRTPDSR
                     YFDELERLIDAVHERGVIHLDLRNRRNYGIDDMGRPYLVDFASSLYLPNKGLLWRLLR
                     HIDLMGLAKLKAKVNPALIRPGERRAHALGRTLSRLWIPPRVLRFVRGWLSGILRRP"
BASE COUNT         1517 a         2630 c         2884 g         1868 t
ORIGIN      
        1 gaaccgagaa tcgtcgcagc atatcattat agcatgtcgt ccggagatgt caatcgacaa
       61 tcaacgggtc ccccacggcc gacgtggttg accggaaaaa ggcttcggcc tctcactctg
      121 gccggggtga agcctcgaag gtgcgcgggc ctttcatcac cctccaccag gctatgccgt
      181 ggatgccgtc gtggaggagc tgcatgtcct tcacgtcgaa gggcaggggg acggcggggc
      241 ccacaccctc aagcggtatg acgcgaggtg ccttgcgaag gtccacgagc gcttccctgg
      301 tgaggaagag atccctgccc actgcatagg ccgggtaggc actatccagg aggtccttgc
      361 ccatggtgtg ataggcgaac cccttgggtg ccgtgagctc gacgaccgtc gggaccacgt
      421 cgatgtggca gccgggtgta tgtatgacgt ggggtgtgtc tctgggaagg caccgcccgt
      481 acatgacgaa cgggatgagg tgccgctcga gggggtccgg gttctcggcg agccatctcc
      541 tcccgggatg gtcggcggtc acgatgaaca agctttcctc gtaccgggat gacgcctcct
      601 tgcaaaagag caggatgcat ctgtccgcgt accagatatg cccgagaaac cgcgcgtcat
      661 cgtcgtcgat gggtatcccg tcgatcgagg aggggagtct ttcccgatgg aaccccttgg
      721 atgggaggtc cacggtgtac ggggggtgga gactcgtggt gaggatgaga ttgaagctcg
      781 gcacctcgtc tttcacggtc ttcagcacga aggagaagat gtgctcgtcg tccacccccc
      841 actcgttcgc ggacaaccag ctgcccatct cgccgcctcc gtagacctcg tcgaacccct
      901 ggtccttgag aaaatcgccc acgcgctgcc acgtcaggta tcccccgtag aacgccctgg
      961 tccggtagcc aaggcgttcg aagatggtcg tgatcgatgt ggggtagggc ctcttggagc
     1021 tcatgcggta gttggtctgg atgtcggcgt cgggcaggcc cgtcacgatg gtgttgaggc
     1081 tcgacatggt cccctgggtg gaggctacga aggggcggca ggagatgccc tcgtgtgcca
     1141 gctccttgag cccgtccgcc acgtggaggg aggcatactt ctcttcgagc ggccacgcgc
     1201 tgtagctctc ggccacgatc cagaagatgt gcgccggtgg tcgggcagag gcgccctttg
     1261 ccgtcctgag gaggctcgcg tcgatgtccc cgggtaccgc ccgagggccg tacacgcgcc
     1321 tcaaggcctt ggccacgtcg ccgtcgggga ggtactcccg gacccccttc tccctcgaga
     1381 tcttgatgtg tcgctggatg gcgtagcgca gcgaggaata ggggttcaaa acggtcttgt
     1441 tcaggaagtc gtccgtggtt atcccggcat gccgctcccg ggccggcatg gcgccgatgg
     1501 accctcgtaa cccgaacacg atggccgcca cgatgaggac ggtcacggtt ctacgcaggg
     1561 ccgggcgtga ggcgatacga tcgcgctcgc cctgctcgag caggggggtt tgaagaacac
     1621 gcctgaggag gatgcctgcg agggtgaccg tgacgatgaa gacgacgagg tgcgtggagg
     1681 catggtaggt cgagaggatc gtcttgaaga tagccgccct gtcgtcgtag atcaggccga
     1741 aaaggtagtg gttgaactgg tcgttgtact cccggaaata ctcgatggtg atcaggcaca
     1801 acgcgctcga caggcacacg aagaccagcc ctgtggccgc tcggaccctg ccggcgagca
     1861 cgcgcacgtt tttgaagata caggccgtgc tgaagcagaa tgggacgagc atgaaggtgg
     1921 tggaaacctt tgcgtcgtaa cgcatgccgt tcaagaaggc cgacaggatg gtcgcgaagg
     1981 tgcttgtcgg ttccatccgg tggcggaaga cgacgaggaa ggcgatcctg aagcacgcga
     2041 aaaaggccat gcagaagacc cagagcacga ggtctttctg ccagctttga aagaagcggg
     2101 accatctctc catcgccgac cttcacgtgt gtttcccgcg atcgaccggc actcgccaag
     2161 agcgcctgtc ggtcgatccc gacgtatcgt tagtatggcc tgtgcatacg ggtcaagggg
     2221 tcaatggggt gacgtcgaca ggggggggcg gtgagggatg ccgggtcccg gcgacaaggc
     2281 gctgtccgtc ttcgacgggg aaacggtgcc ccatcctgca atcgccgcga cggatcgcgc
     2341 tgtcggtgac cgcaccctga ggctttccgt gggccgccgg gcacgcgcag gctcgtgcgg
     2401 cttcaaaaga gggccgcttt catgctactt gcacacgggt ttcgaccccg gcgccagggt
     2461 tgccagcagc cccgccgcag cctgtatgtc ctccgtgccg aggcttgcgc ccttcgccac
     2521 catccgcttg atggtccgct cccaaccggc ggcgtccctc gtgccgagcg ccctgcacac
     2581 ccgggccgtg tcgtggcacg ccgagcagga cctcatgacc gtggtcatcg tgtccttcgt
     2641 ggccggagcc tgctgtgctt gtgcgaacga aaaggccagg atcgtgcagg ccaggatcac
     2701 tgccccgaac gagatccacg tggtacgcaa cctctcctct ttcttgccca tgcggcgcct
     2761 cctccaggcg gtgttgcgat atcctcttca tatagcggcg tcctggagga cttgcaaacg
     2821 acgggaaggg attccccgtg cacgagcatg ccgccctgcg tttccgcccc tgtcaggctg
     2881 catgggtgcc gggggtgacg tgcgggacgg gacggcggcc tcagtgggta tcgtgggatc
     2941 aaggtcgaga cgatgcgccc tcgagtctta ccgtgacgca ggagacgaaa cgactgtcca
     3001 gcgtccgggt gtcgaggatc ccaccgagct cgtcctcgat cctcaggcag agggcccggg
     3061 tcacgagttc ccggatgcgc cgctctgcat cgagcaagag tgaggccaga tagacgccga
     3121 ggtctcgctc gaaacggttt ttcacggtcc tcttgccccc gcgcacgtgc gtggccgagg
     3181 cgacaaaggc gaggaactcg tcctttgtta gcggccggaa cccggtttcc ccggtcaccg
     3241 cgttgagggc tgcggccgtg aggatgaccg tctccaggtc gagggccgta cccgtgttgg
     3301 tcccgccgag agagggtatg gccctctccc attcgagggt gtccgacacg gatgccatgg
     3361 cctccagggt gtccaggacc ccggtcacct cgtctatgtc ctcgggggaa gcgaattccc
     3421 tgtccttgta cagcggcctc ttcctgagga gaccttccac gatcccgttc atgcgggtcg
     3481 gtatcatgga gcgctccacg gagtcgagga tgcgcttgag acgatgctgg agctgaaggg
     3541 cctcgttgta gccgatcgag aagagggtct cggcgttgac ggtctcgagg atgacatgag
     3601 caggaagccc tttctccctc atcgcgacct tcagccccag ggtgcacaga cagcccgcct
     3661 tgtccatgcc cgccctcagc tcgtcgctgt cgttgagggg cttgtagtcg gccatgagga
     3721 tcttgttgga gaggtaaacc atctcgtaca cgaagcggtc gcgggtggca tcgctggcgc
     3781 gtccgagggc cttcgccagg aggcttgtgc ctcccgggaa actctcgagg tactctgccg
     3841 gcagcatgtg gagatgcctg gtcaaaagcg gcgtcttgtc cctgtggatc cccagggaca
     3901 agagcctgtc gatgcttgcg cggcggtata tctcaacgga ttcttcaggg ctcgggaagc
     3961 ccatctcgac gagcctcaag gtccttttct cgtacgacgc ctcctcgagc gagctcttga
     4021 gctcgtacat gatcgcttcg aggagggtga cgtagaggtc gtgcctctgg gtgaacaggg
     4081 tgctcaacac ctccttgagg aactgggcgt gatcctcgtc gcccgtgatc ctgtagaagt
     4141 agacgttgtc aatgctctcg aacccctcgt cgatgaggtc ctggatgttt tcgtcggtcg
     4201 gcctggtgtg gaccacctcg aagcactgct ggaagatcaa gaccaggatc tccatgtcga
     4261 gggcctccac ggcctggagg aaggtttcgg gggaggagag ggacagttcc tggagccatt
     4321 ccatggcgga gtcgacggag aaggagtccc tctgccagca gtcgagatcg atgaatcggc
     4381 agacggtctc gggtggtacg tactggagca ggatctggct gtccatcccc caggattcct
     4441 tcatgatgag gtacgcctcc tggggaggga gctgctcgag tatctgcctg gtgtacggcg
     4501 actcgatgat gcccctggcc cgctgggccg gagtacatgc cagcacccgg gcgatctcgg
     4561 ccttcagggt gggtctctgt gccatggcgg tgtggttagc acacctgtga ggggcctgca
     4621 acaagcggcg ggctcccttt gccaggggga tacaacctgt ccggtgcttc gctagagggt
     4681 gcccatcgat gcgagcagcg atgccatggc cttggcctgg tcagccctcg cggagatgag
     4741 attgttgcgg gcctgagcga gcagcgtttc agccctgaga acgtccgtcg aggtggaaac
     4801 ctggaacgag tagcgttctt tctctatcct gaagttttcc tgggcctggt ctatcgccct
     4861 ttccgccacc ttgagccggg cctgcgcctc gagcgtgttc aggtatgcgc tcttgacctc
     4921 gagggtgacc atgttcttca gggactcgag gagatattcc gccttcgctc tcgtgtagga
     4981 ggccttgttg tagccccaga cacccgctcc accctcgaac aggttccagc tcaggcccag
     5041 gcccaccttc caggaatcct cctccacgtc ggggtcctcg ccggacctgg tgtatgtgta
     5101 ggtggcggcg acgttgggca tgaaccgcga cctcgcgatg gtcacgccct tgtgcgcact
     5161 gtcgagctgt gtcttggcca ccctgatctc ctgtctgttc tcgaacgcct gttcgatggc
     5221 ctgttcgaga gtcatgtcca cctttgcgac cgggatttcc tccgcaatgg aaacctcctc
     5281 ggaaggggcc ctcgccagga ggaggttgaa gcctgtctct gcgatccgga cagcgttttc
     5341 cgccgttatg aggccctgct ccgtctccgc gtagcgcacc tgcgcctcga gcaggttgtt
     5401 cttggggatc atcccctggt cgaagaatgc ctgtgacatg tcgagcaggg acttgacgga
     5461 cgccatgctc gatttcgcca cctcgagcat ctgccgcgcc ttgatcacgc cgtaataggc
     5521 gtctatgacc tggagcttga gctgccggga ggcccgttcc ctttcgatca tggaggcatg
     5581 gcgttcgttc ttcgacacga gataggcgtt gtagagggcg cctcccgcga acagcacctg
     5641 cttcgcctgg aggctgagct cgtagttgtc cttcgtcccc atgggtatgg tgagcggttc
     5701 ggccaggggg acggttatgc tcggcgcctc gttcaggcgc acgtagccgt acgacacgtc
     5761 cgcgctcggg agcatgcggg caagctgaga gcgctcctcc atctccttga tcgaaacctc
     5821 ctggtcctgg gcctttatct gcgggttgtt ctccaaggcc acccgcagtg catcctcgag
     5881 ggtcatcgag ccggccttgc agtccaccgg cgccatgatc aagacagaga cggcgagggc
     5941 gatcgctgaa cgtatggtat ttctcatgtg cctctcctca tgtcgggata cacgcttcca
     6001 tgacatggca cagccttcga tggttctctt cgggcttgct cgcaaagagg atatgacaaa
     6061 gcggtgcgcg agtcaaatga acacgatcgc gaccaggtca agcgtgtcgt gtcgttcgtg
     6121 ccccgggtct cgtgacacgc ccttcgtgca cggcccggga tgagggcccg gcgggagaga
     6181 aaagcctgtg cagaccccta tccctcccgc ccaggggacc tttcctcgat cacgatcgag
     6241 ggcaacgggc gtggcgcccg agaaccccgt ggcgccgtga accaggacac cccgtgcagg
     6301 aggtctctac ccgatcgaat ggaagacgag acccgttggt accttggggt agaagtacgt
     6361 cgagttgtgg ggcatcctga ggcctgcctc cgcgatctcg aacacctcct cgatcgacgg
     6421 cgggttgagc agaaaggcta tgtccgcctg gcccttgctc accatgtcga gggcctcctc
     6481 cgccttgctc gtgtaagaaa tgcgcctgtt ctctatgttc gcgttgatgc cgaggaccgg
     6541 ttcgatgacg caatcgtgga gtatggtgac atcgtttttc ctcagctggt ggggtatgtg
     6601 ctgaggcatc atcctttcga ggtcttcctc gtgtgcgagc tgcaggaggt aaaaccgggg
     6661 gatccccttg acgaacagcc ccaccgcccc cttgcctgcc ttgttgagcg cggagatgaa
     6721 ccccttgcgg ttgtcgtagg ggatcatgtt gtagatctcc ttgatacggt actcgaggtc
     6781 cacgaggccg acccccatgc tgtcgaggat gacacgatgc atgggaagga tcttgacccc
     6841 ctcatccacg ttcacgaggt acatctgtat gtagtcgaag ttctggttgc cgtccgactt
     6901 gcctgtggcc gccctcatcc tgtccctgta tatcttggct gtttcgtagc ggtgttgtcc
     6961 gtccgccagg acgatcttct tgttctgcat caggcgggag accttgtcta cgagggcggt
     7021 ctcgtcgatc acccagagcc ggtgcgtctc gtcgcggaag gtgaactcga cgtgcgggtc
     7081 cccgagcctc ggtttcacct gggtctcgat gatcttcttg ctgtctgcgt agaggccgag
     7141 cacgctgctg aagttcattc tcacctcgtt catgaggcgg agcctgtcca gcttgggact
     7201 cttgaccgtg tgctcgtgcg gcctgatcac cttcttgtcg tcgtcgtcca ggcgcacgga
     7261 cgcgatgaac ccctttcgcg tgtaggtctt gtcgccgagc gtgaaggtgt gctcgtggta
     7321 gtagatcgcc ggcttctcgt cctcgatgag gaccccctcg gagagccact ggtggaagta
     7381 gtccctcgcc cgggtatacc ggttgttgtc cttgtcgtcc ttggggtgct gcttgcccag
     7441 gacgagcctc acggcattgt aggggctcct tgcatagagc tcctcaagtt gctgattggt
     7501 gatgacgtcg tacggcggac agatgacgct gtcgaggccg atacgttctg ggttgtacct
     7561 gatgccccga aaggagcgga tcttgggcat gtcctgcacc tctcacggta ggtcagactc
     7621 gtagatacag aatgcgctat atatatgcgg tcttgacaaa tttcaacagg gaaatgccgc
     7681 cgttgttgtt cccgcctgcc gttgtccctg gaaatgggca ccggcaggcc gctctgccgt
     7741 atgggcggtc acggcccggc cttgccgtgt atcgtggtcg gcgcgatcgc cggatgccct
     7801 ggagcttgcc ccgctcgagg atgcccgggg ggtgaggtcg catggcctca gggattgcta
     7861 tacagtacct gaaggggtat acgtggtgca tgaggtatgc agggctggca ctctcgatgg
     7921 gcgtgctcct ggcgttcatc gtgttcctcg tgaacaattc ccaggagttc aggctcgggt
     7981 tcatggggta cgaactcgag gcgcctcttg cgctctggat cctcatgctc gtgttcttcg
     8041 ccgcggggac attgcctgtg ctcgcaacgg aggtcttcag gtccgtccgc acgagggtga
     8101 gaaggcgagt cctcgaggcg gagatccggt ctctcgagga ggaactctcg agcggctcag
     8161 ggcctgcgaa ggattcccga gagccatccc cggacaaacc gtaagacccg cgggggtatc
     8221 cacagcctgc tgagcgtcct gcccagcgca tgtgcacggc gttccccggg ccttatgaga
     8281 gcggggttca ccttcgcctt gagctttgcc agacccatga gatcgatgtg cctgaggagc
     8341 ctccagagga gccccttgtt cggcaggtag aggctcgagg cgaaatcgac gaggtacggc
     8401 ctgcccatat cgtcgatccc gtagttcctg cggtttctca ggtcaaggtg gatcacccct
     8461 cgctcgtgga cggcgtcgat gagtctctcg agctcgtcga agtatcgact gtccggcgtc
     8521 cggcgagcac gggccctgag gttgccgccg cccatgaagg ttgtcgttat ggtgtacctg
     8581 tccgggctcg tggcgagcac cgggatgcct gggatcccgg agagcttgga atagacgaag
     8641 gtctcccacc gtatgaggag acgtcccgcg accctgaccg gaagaggtct gtcgcggtag
     8701 gttttctcga cgaggaggat gccgtctttc tcgatgaggc ggacatccgg gccgtagata
     8761 cgtctcctct tgaggaagat ctccccttca gaatggttcg gcgatgccac gggttccatc
     8821 attgcatgat tctcggggcg gatggtaggg ttttctcgcg atgagaaggg cttcgaagac
     8881 cagcatgaag ggcaggctc
//
LOCUS       DAOSCR010000022         8797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5216_length_8797_cov_3.365363, whole genome shotgun sequence.
ACCESSION   DAOSCR010000022 DAOSCR010000000
VERSION     DAOSCR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5216_length_8797_cov_3.365363"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLT69_01245"
     CDS             complement(<1..1089)
                     /locus_tag="PLT69_01245"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOJ13107.1"
                     /translation="MNFDKVALHVPSILVPNPQIDYTKWSVIACDQYTSQPEYWREVE
                     RIVGSSPSTLRLILPEAYLGDTADETALEAIKKTMDIYLADGTLQPIGEGFVLVERTT
                     SGGSLRRGLMVALDLESYDFSQGSSSLVRATEETVLERLPARVKIRSRASVELPHTMV
                     LIDDDAMSVIEPLFGRPLEKIYDFSLMMNGGRVRGYRVTDDRTITSVARSLEALLETS
                     RRRAGAGGAILYAVGDGNHSLAAAKLFWEEIKNLPGASGHPARYALVELVNLHDRGLV
                     FEPIHRVLFNVDPGRVLEALADFFTGMRCTCTVTGEPPAPTRPQGGTQLIRYVSGKGR
                     GTIRLERSPWVLEVAGLQAFIDAYVKENP"
     gene            complement(1183..>3516)
                     /locus_tag="PLT69_01250"
     CDS             complement(1183..>3516)
                     /locus_tag="PLT69_01250"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOJ13108.1"
                     /translation="LHDLAMAMGYVHASDRLAQMVGFKLLAQGRISELTGPATFQVDV
                     YMRTLGLSEAAQAMFEGISPENRALLESYAQGVNAYIQLHKGRLNPALGLAGYTPEPW
                     RPMDTAYVFALLNTALSFNLVEEIAALKIMQVVGPRRAAWLVPVYPDEPIPFDEAEKL
                     ARVDLARLPVSDIDIASTMTTLKSLGLSGLPASNNWAISSRRTAKAASILANDTHLLI
                     SLPSLWCMMHLRCKDMNAAGVSIAGAPCIAAGFNGSLAWGMTMVMADNQDVFLERLKV
                     IDGRPHYLYQGRWEPVQTRTETIRIRGEDPRTIQVNRTRHGVLPDGALSARPILPFQP
                     RETGLPYGLALSQVQAGDTDGTLQAFFELNLARSVEEALPVTRRIRAIALNMVVADRD
                     SIAWRVTGTYPVRARGRGLLPSPGWTGEYDWKGYVDPASLPESLNPPQGYILTANNRT
                     VPADHPVTLSSSWYWPERAERIEQMILATERHSLQTSMAMQLDTHSAFVPKLKKVYLE
                     GETGREIARVIESWGDARARAKARQGLEMLRAFDADMSASSPGAALVGAMLHCATRAI
                     FLDELGPEDSPAWRAFLALGDENYNATCDHIVARTDESPFWDDCSTASREDKAQILAR
                     VIGEAYELCERRLGTNHAAWRWGRLHAYTWETDAYRMTEHLDPVRRLFMSALWGYFNR
                     GPHEAPGDHFTLNVAAYTMGRNFDVWLIPAMRIVVDFGLQEPMHAVNSTGQSDKPSSP
                     HYDDATALWRQGRYLAFPFGEEGIRSRFGNVRKLVPR"
     assembly_gap    3517..3526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4167..4949)
                     /locus_tag="PLT69_01255"
     CDS             complement(4167..4949)
                     /locus_tag="PLT69_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOJ13109.1"
                     /translation="MDIPIYIVDAFTPRLFKGNPAAVCPLERWLDDGVMQSVAFENNL
                     SETAFFVREGDGYRIRWFTPRSEVGLCGHATLASAHVIFTEMDPTRDLVVFDSAGGPL
                     SVRREAEFLAMDFPSLPPRPAIPPQGLVEGLGVPPLEVLEGGDYLAVYACEEDVKNIA
                     PDMDLLKDLPLRGVIVTAPSDRCDFVSRFFAPGLGVNEDPVTGSAHCELVPYWSARLG
                     KRDLHAIQVSERTGELFCRDNGDRVAISGRAVTYLRGRITLP"
     gene            5229..5516
                     /locus_tag="PLT69_01260"
     CDS             5229..5516
                     /locus_tag="PLT69_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13110.1"
                     /translation="MIFDNERLLRIASQRHEGHEIDALVAQRDRFLSEHPELRALQEE
                     IDALLATTLDPIKRLDILFMLISERLDAMRSSLGEAFEAAGRVCGSLTRDR"
     gene            complement(5953..6486)
                     /locus_tag="PLT69_01265"
     CDS             complement(5953..6486)
                     /locus_tag="PLT69_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13111.1"
                     /translation="MTTRRHLMLLALVLILASCTSRTGGDISTKGNIVEIKAKDLRVK
                     ALKGATEEGVYVLMSAHAVEHPPALSYLDGKLTVLPKEDYELLGKLRANPDPENRNKI
                     RELRKKVRRLAVIAEDAQTQKKIQELIQKAKRQEYPVVAITMTEILVMELKYQGKPIY
                     MKGEAAKNHLVSKIKVL"
     gene            complement(6711..7496)
                     /locus_tag="PLT69_01270"
     CDS             complement(6711..7496)
                     /locus_tag="PLT69_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOJ13112.1"
                     /translation="MTIEIEATLAVCCQDPDGVLEAVYALTALGPYRLEPSGVSVLED
                     TYYDTPEGLLARTACALRTRRERGNTLVCVKKKERTTMGLLAEREETETPWCDRRFDA
                     VARLLSEICPSRPLEEGLFDPTDPEATLARLGYFPVQSRQTVRRVLVARHRDAPPGEM
                     TAKVCLDRVTYRIMGRNLVHREIEVEAGGGTDAAHAAAIAGLLRKRLGGVLFPWPHNK
                     LLTGFAISELASCGLLRPETFERGLSRGDYKLISRRLAASPRT"
     gene            complement(7943..>8797)
                     /locus_tag="PLT69_01275"
     CDS             complement(7943..>8797)
                     /locus_tag="PLT69_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOJ13113.1"
                     /translation="MQADIKRRISKHKEEAQKKKGGVKQKNVYAIDKEGAAQVVLLGG
                     PNTGKSSLVAALTNAEPEIAPFPHTTHKPTPGMAPYENIQFQLIDTPPVTLEYADPLM
                     MDLVRRADIVTMVLDITTDPLGQYEDLLAIMADHRILPLGSCVPEGMRKPVTFKKVLV
                     AVNKVDCPGRQEDFDTFLELTQTSLPVVAVSVLNSTGLDAFLKKIYDLAGIIRVYSKT
                     PGREPDLEEPFVVRAGSTLEELAGRIHKDFVEKLKYARIWGGTVHDGMMVQRDYVLQD
                     GDIVEIHI"
BASE COUNT         1418 a         2828 c         2872 g         1669 t
ORIGIN      
        1 ggggttttcc ttcacgtacg cgtcgatgaa cgcctgcagg ccggcgacct cgagcaccca
       61 gggggaacgc tccaggcgta tcgtccctct tccttttccg ctcacgtacc ggatgagttg
      121 ggtgccaccc tgcggtcgtg tcggggccgg gggttccccg gtcaccgtgc aggtgcaccg
      181 catgcccgtg aagaaatcgg ccagggcctc gaggacgcgc cctgggtcca cgttgaagag
      241 cacgcggtgg atgggctcga acacgagccc cctgtcgtgg aggttgacga gttccaccag
      301 ggcgtagcgt gcggggtggc ctgacgcgcc agggaggttc ttgatctcct cccagaagag
      361 cttcgcagcg gcgagggagt ggttcccgtc gcccacggcg tacaggatcg ccccgcccgc
      421 gcccgcgcgt ctgcgcgatg tctcgaggag cgcctcgagg gaccgtgcca ccgacgttat
      481 cgtcctgtcg tcggttacgc ggtatccccg aacccttccg ccgttcatca tgaggctgaa
      541 gtcgtagatc ttctcgagag gccttccgaa gagcggctcg atgacgctca tggcgtcgtc
      601 gtctatcagg accatggtgt gcggcagttc cacgctcgcc cgggagcgga tcttcacgcg
      661 ggccgggagc ctctccaaca cggtctcctc cgtggccctg accaggctcg agctcccctg
      721 ggagaagtcg tagctctcca ggtcgagtgc gaccatgagc cccctcctga gggacccccc
      781 ggaggtcgtc cgctccacga ggacgaaccc ttctccgata ggctggagcg taccgtccgc
      841 aaggtagatg tccatggtct tcttgatggc ttccagggcc gtctcgtccg ccgtgtcgcc
      901 caggtaggcc tcggggagga tgagcctgag cgtcgacggc gaggagccca cgatcctctc
      961 gacctcccgc cagtattccg gctgcgaggt gtactggtcg caggcgatca ccgaccactt
     1021 cgtatagtct atctgagggt tcggcacgag gatgctgggg acgtggagtg cgaccttgtc
     1081 gaagttcatg gcgagtcacc tcgtcgatgt gggtgtatcg ccatcacata gcagaatcag
     1141 ggacaggctt tcaatcgcct tgccgcgggg tcgtcaaacc cttcagcggg gaacgagctt
     1201 cctgacgttt ccgaagcgtg acctgatccc ctcctccccg aacgggaagg cgaggtagcg
     1261 tccctggcgc cacagggccg tggcatcgtc gtagtggggg ctggagggtt tgtccgactg
     1321 gccggtgctg ttgaccgcgt gcatgggttc ttggagaccg aaatccacca cgatgcgcat
     1381 ggccgggatg agccacacgt cgaagttcct gcccatggtg taggcggcca cgttcagcgt
     1441 gaagtggtcc ccgggcgcct cgtggggacc ccggttgaag tacccccaga gggcggacat
     1501 gaagaggcgc ctgacgggat cgaggtgctc ggtcatcctg taggcgtccg tctcccacgt
     1561 gtacgcgtgg agccttcccc acctccacgc ggcatggttc gtgccgagcc tcctttcgca
     1621 cagctcgtag gcctcgccta tcaccctggc caggatctgc gccttgtcct cccgggaggc
     1681 tgtcgagcaa tcgtcccaga aggggctctc gtccgtcctc gcgacgatgt gatcgcacgt
     1741 ggcgttgtag ttctcgtccc ccagcgccag gaacgccctc catgccggtg agtcttcggg
     1801 cccgagctcg tccaggaaga tggcgcgcgt ggcgcagtgg agcatcgccc ccacgagtgc
     1861 cgcccccggc gaggaggcgg acatgtcggc atcgaaggcc ctcagcatct cgagaccttg
     1921 gcgggccttg gcccgggcac gcgcatcacc ccaggactcg atgacccttg cgatctctct
     1981 gcccgtctct ccctcgaggt acaccttctt cagcttgggg acgaaggccg agtgcgtgtc
     2041 gagctgcatg gccatgctcg tctggaggct gtgccgttcg gtcgccagga tcatctgctc
     2101 gatgcgctcg gccctctccg gccagtacca ggaagaggac agggtcaccg ggtgatctgc
     2161 cggaacggtc ctgttgttcg cagtcaagat gtatccctgt ggcgggttga gggactcggg
     2221 cagggatgcg gggtcgacgt aacccttcca gtcatactcg cccgtccacc ccggggaggg
     2281 caggagcccc cttccccttg cacgcaccgg gtatgtaccg gtcaccctcc aggctatgga
     2341 gtccctgtcc gcgaccacca tgttcaaggc gatcgccctg atcctcctcg tgaccggcag
     2401 ggcctcctcg acgctgcggg cgaggttcag ctcgaagaag gcctggagcg tgccgtctgt
     2461 atcccctgcc tgaacctggg acagcgccag gccgtacgga aggccggtct cccggggctg
     2521 gaagggcagg atgggcctcg cgctgagcgc gccgtccggg agcaccccat gacgggtcct
     2581 gttcacctgg atcgtcctcg gatcctcccc ccgtatgcgt atcgtctcgg ttcgcgtctg
     2641 aacgggctcc cagcgaccct gatagaggta gtggggcctg ccgtcgatca ccttgagccg
     2701 ctcgaggaag acgtcctggt tgtccgccat caccatggtc atgccccagg cgaggctgcc
     2761 gttgaacccg gcggcaatgc agggtgcgcc ggctatgctc acgcctgcag cgttcatgtc
     2821 tttgcacctg aggtgcatca tgcaccacag ggagggcagc gagatcagga ggtgggtgtc
     2881 gttcgcaaga atgctcgcgg ccttggccgt gcgccggctg gagatcgccc agttgttgga
     2941 tgcaggcagc ccggaaaggc cgagggattt cagggtcgtc atggtggatg cgatatcgat
     3001 gtcggagacg ggcaggcggg cgagatccac ccgggcgagc ttttccgcct cgtcgaaggg
     3061 gatgggctcg tcggggtaca cgggcacgag ccacgcggcg cggcgcggcc ccacgacctg
     3121 catgatcttg agcgcggcta tttcttcgac gagattgaag gagagggcgg tgttgaggag
     3181 agcgaacacg tatgccgtgt ccatgggcct ccagggctcc ggcgtgtacc ccgccagccc
     3241 gagcgcgggg ttgagcctgc ccttgtgcag ctgtatgtag gcgttcacgc cctgggcata
     3301 gctctccagc agggccctgt tttcgggcga gatgccctcg aacatggcct gtgcggcttc
     3361 cgagaggccc agggtgcgca tgtacacgtc cacctggaac gtggcaggtc ctgtcagctc
     3421 cgatatcctg ccctgtgcga ggagtttgaa cccgaccatc tgggcgagcc tgtcggatgc
     3481 gtgcacgtat cccatggcca tggcgaggtc gtggagnnnn nnnnnnaggc tttcggcctc
     3541 gatgagcggg atcccgaagt cgtcccttgc cacggtgacc gtgttctcga gccccggcaa
     3601 caccagggtc ccgcactcgg cctccacggt atcctcgaag tgcctggtga tggcgccggt
     3661 acaagccccg atcatgcagg cgagcacgaa ggcgagcccc agggagagta accccggaca
     3721 tacgcgggaa tgtctcgcag gacgcgaacc gcaagcctca gcgggtaatg gagagtgcag
     3781 ggtggcttct cccttttccc cggacacctt cgtgacctcc tcgtatcctt ccatgccccc
     3841 actggaagcc gtcttccctg tgcatggcgc tgtggcggcg aggatcgggg agagtgcccc
     3901 gcccgcgccc ttcagcctcc gtcacaccct caaaggcccc gccttccccg gcccgggtgt
     3961 caccgacggg cccatggtgg gttcgttcag ggccggcggc ggtcaaaggt tccgcacgtc
     4021 cgacgagggc gcatcgcaat gcctctccca ggtccacagc cgcctttttc cctccggggc
     4081 ggtccggaga cgttcctcgt aggtggcgga aacctcgagg gcccgccatg gccggcttcc
     4141 gcatcgccca cccggcacaa cgcctttcac ggcagggtta tgcggccgcg aaggtaggtc
     4201 accgcccgtc cggatatggc gaccctgtcg ccgttgtccc tgcagaagag ctctcccgtc
     4261 cgctcggaga cttggatggc gtggaggtct ctcttgccca ggcgtgccga ccagtaaggg
     4321 acgagctcgc agtgcgcgga tcccgtgacc gggtcctcgt tgacccccag tcccggtgcg
     4381 aagaaacgcg acacgaagtc gcagcggtcg gaaggagcgg tcacgatcac ccccctcagc
     4441 gggaggtctt tcaagaggtc catgtcgggc gcgatgtttt tgacgtcctc ctcgcacgcg
     4501 tagaccgcga ggtagtcgcc cccttcgagc acctcgaggg ggggaacgcc gagaccctcg
     4561 acgagcccct gcggggggat ggcgggtcgc ggcggcaggg acgggaaatc catggccagg
     4621 aactcggcct cgcgcctcac ggagaggggc cccccggccg agtcgaagac caccaagtca
     4681 cgggtggggt ccatctcggt gaagatcacg tgcgccgatg caagggttgc atgcccgcag
     4741 agcccgacct cggagcgtgg ggtgaaccag cgtatcctgt agccgtcccc ctcgcgaacg
     4801 aagaacgcgg tctccgagag gttgttctcg aacgcgacgc tctgcatgac gccatcgtcc
     4861 agccagcgct cgagggggca caccgcggcg gggttgccct tgaacagtcg cggggtgaag
     4921 gcgtcgacga tgtatatggg gatatccacg tccttttccc tcccatcgag acacgaaaaa
     4981 agccccgttt gagttcctcg gttcgcgtcc catcgtccca ccagggcgta cgaacatcat
     5041 acaaccagtc gggccgtggc tcaaggatgt gaagaaaaaa gggacaatgg tggaacggga
     5101 agaccataca cccaaggtcc ggaaaggacg tggtgttcct attcatgcga aatccttgat
     5161 gatatcgagt ggcacgtcct ttgctctgtt ttccctgcgg ttcatgaagg gtaagggggc
     5221 cgaatgacat gatcttcgac aacgagagac tgcttcgcat agcatcccag cgtcacgagg
     5281 ggcacgagat agacgccctc gtcgcccaga gggaccggtt cttgagcgag caccccgaac
     5341 tcagggccct ccaggaagag atcgatgcgc tgctcgccac tacgctcgac ccgatcaagc
     5401 gtctcgacat cctcttcatg ctcatctcgg agcggctgga cgccatgagg tcgtccctgg
     5461 gagaggcctt cgaggcggcg ggcagggtgt gcgggtcgtt gacgagggac cgctgatctg
     5521 agagtgtggc gcgcggtctc ttcccggacg tgctcgagag tgccgcggcg gtgacggacc
     5581 gcacaccccc acagggcagt ggctcgcctc tgtcggccgg cggcgtgcaa aggccatggt
     5641 cgatcgtccc tgccctccgg cgcccaccca cgatgacgac gcaccccgcc cgaagggctg
     5701 cccactttct tcaaggggat ttcactgcaa ggagctcgtt cacggtctcc ggccggcggt
     5761 gcgggcaccc actctccccg ccggaactcg gggattacac tgcaaggagc tcgttcacgg
     5821 tctccgggcc cggtacccgg cccgtccatc gagatccgac cgtcctcatg gacagcttcc
     5881 cggcgtcggg gcttgcgccg tcatgccgcg acagggggag gccgggggcc tcgaggccgt
     5941 gacgggtgag gcctagagta ccttgatctt gctcaccagg tggttcttcg ccgcctcgcc
     6001 cttcatgtag atgggcttgc cctggtactt gagctccatg accaggatct ctgtcatggt
     6061 gatggcgacg accgggtatt cttggcgttt cgccttctgg atgagctcct gtatcttctt
     6121 ctgcgtctgc gcgtcctcgg cgatcacggc aaggcggcgc accttcttgc ggagctcgcg
     6181 gatcttgttc ctgttctcgg ggtccgggtt cgcgcgcaac ttcccgagga gctcgtagtc
     6241 ttccttgggc aggacggtga gcttgccgtc caggtaggac agggccgggg ggtgctcgac
     6301 tgcatgcgcg gacatcaaca cgtacacccc ttcttcagtc gcacccttga gggccttgac
     6361 cctgaggtcc ttggccttga tctccacgat gttccccttc gtggagatgt ctcccccggt
     6421 cctggaggtg caggatgcga ggatgaggac gagggcgagc aacatcaggt gccttcttgt
     6481 ggtcatctcg aataacctcc ccgtacggag catctgtcat ctcggcctgc gtgcgtgccc
     6541 agagacgcgg tcccacgcag ccccctgcac ctatagccag gggtgtcagg aatgtcaagc
     6601 cggcaagcct gtgtggtcag gacccctgga caccgagtcc cttcgatgcg cttgagcatg
     6661 tccccttggg gtgcgtccat gtgggcgcgc tcgtccccca ccgggccggg tcaggttcgg
     6721 ggcgaagcgg caaggcgcct ggagatcagc ttgtaatccc ccctggaaag gcccctctcg
     6781 aaggtctcgg gacggaggag accgcacgat gcgagttccg agatggcgaa ccccgtgagg
     6841 agcttgttgt gaggccatgg gaacaggaca ccgcccaagc gtttcctcag gaggccagcg
     6901 atcgcggctg catgagcggc atccgtaccg cctccggctt ccacctcgat ctcgcggtgg
     6961 acgaggttcc tgcccatgat gcggtaggtg accctgtcga gacagacctt ggcggtcatt
     7021 tccccgggtg gtgcgtccct atgcctcgcg acgagcaccc gcctcactgt ctggcggctc
     7081 tggaccggga aatacccgag cctcgcgagg gttgcctcag ggtccgtggg atcgaagagg
     7141 ccctcttcaa ggggtcggct cgggcagatc tccgagagga gccgtgcgac cgcatcgaag
     7201 cggcgatcac accagggcgt ctccgtctcc tccctctcgg cgagaagacc catggtcgtg
     7261 cgctccttct tcttcacgca caccagggtg tttccgcgct cccgcctcgt cctcagggcg
     7321 caggccgtac gcgcgagcaa cccttccggg gtgtcatagt acgtgtcctc gagaacagac
     7381 accccggagg gctcgagccg atagggcccc agggcggtca aggcgtagac cgcctccagc
     7441 acgccgtccg ggtcttggca gcagacggca agcgtcgcct cgatctcgat cgtcatgggt
     7501 cgttgggccg gctcaggccg ccttccgtat gaggagggac aggcatatca ggatgcatcc
     7561 cgttacgaca aagctcgtca tgttcatgac ctctcgacat gaaattgcac caggcattct
     7621 ggacatcccc cctggcatgg cccctcgcaa gaccccgggc ataccagcgg accgacggcc
     7681 gtcatgcgtc gacgcccctc acaagggcgt agtcttctta gacaagcgct ccatgaaagg
     7741 caagtgaaaa agaggtgaag cgagggacgt gggtcggtct tcgactgccc gtgggggtca
     7801 cggccgggat cgcggtctgc cacgcatccc ggtaagcgtc gtgatcgagc gcgccggtcg
     7861 cttgtccccc gtcctgccgg ggtgtgtccc gtcgggcagg tgcacggcgt cccgaaaccg
     7921 atggctccgg ccgtgacggg gtctagatgt ggatctcgac gatatccccg tcctggagga
     7981 cgtagtccct ctggaccatc atcccgtcgt ggaccgtgcc gccccagatg cgtgcatact
     8041 tgagcttttc cacgaagtcc ttgtggatcc tgcccgcgag ctcctccagg gtggaccctg
     8101 ccctgacgac gaacggttcc tcgaggtccg gttccctgcc cggggtcttg gagtagacac
     8161 ggatgatccc tgcgagatcg tagatcttct tgaggaatgc gtcgagaccc gtggagttga
     8221 ggacggagac ggccacgacg ggcaggcttg tctgcgtgag ctcgaggaag gtgtcgaagt
     8281 cctcctgcct gcccgggcag tccaccttgt tgaccgcgac caggaccttc ttgaacgtca
     8341 cgggcttcct catgccttcg ggcacgcagg aacccagggg cagtatcctg tggtcggcca
     8401 tgatcgcgag gaggtcctcg tactggccca gggggtctgt cgtgatgtcg aggaccatgg
     8461 tgacgatgtc cgccctcctc acgaggtcca tcatgagggg gtccgcgtac tccagggtca
     8521 cgggcggtgt gtcgatgagc tggaactgga tgttctcgta gggcgccatg cccggtgtag
     8581 gcttgtgggt ggtgtgcggg aagggcgcta tctccggttc ggcgttcgtc agggccgcga
     8641 cgagagagga tttccccgtg ttgggccccc ccagcaagac cacctgggcc gccccctcct
     8701 tgtctatggc atagacgttc ttctgcttga ccccgccctt cttcttctgc gcttcctcct
     8761 tgtgcttgct gatgcggcgc ttgatgtccg cctgcat
//
LOCUS       DAOSCR010000023         8739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5268_length_8739_cov_3.830263, whole genome shotgun sequence.
ACCESSION   DAOSCR010000023 DAOSCR010000000
VERSION     DAOSCR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_8739_cov_3.830263"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLT69_01280"
     CDS             complement(<1..702)
                     /locus_tag="PLT69_01280"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOJ13114.1"
                     /translation="MPDTFEPRPDETLDVLACGSIGVFQKKRGYRYSLDAYLLAAFVE
                     EEPGTRVLEVGSGSGVVSMMLASLKGLLVTGVEIQEELAEMSRRSVARQGLEGSVEIV
                     CADIRNYTGHKVDAVVANPPFRPPQAGRVNPDRQKAIARHEITLDLEALLKKARDLLR
                     HRGRLYVVYPSWRLADLISAMRAVRIEPKRLKCVHTTWNAASEICLVCGTKGGGRGLE
                     IESPLMVYNDDGSYHD"
     gene            complement(721..1461)
                     /gene="thyX"
                     /locus_tag="PLT69_01285"
     CDS             complement(721..1461)
                     /gene="thyX"
                     /locus_tag="PLT69_01285"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOJ13115.1"
                     /translation="MKYFVHEYNHIEKWKHLRQDLFRNEEPNLRLVGLTVPTKDLVDQ
                     GVTTETLPAYTARQSHESEGTHEDDLRLNRKLVSWGHHTCLEAVQFVYLVTGVSKSLA
                     GQWTRHRIGIGWTFRSTRYVKANKNTFVYPALEYLDSEDQVMGIYRAYEREHRRAIEV
                     YEEIRAMGVRNQELRRLMPVGWATAAYVYVNARALRHFFELRLDTSAEWEIRRLAYLM
                     FNDAMTHAPSLFEDMLERAGASPGGHAS"
     gene            complement(1458..2042)
                     /locus_tag="PLT69_01290"
     CDS             complement(1458..2042)
                     /locus_tag="PLT69_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13116.1"
                     /translation="MVIGADRIIEMIRSGVDLEDPVSRRIVRKPLIEDIGQDQVDGIE
                     GTTVDLRLGAVYRPVGRARLMRDVRVTPEIVKLVDGAQDPDAVLRVTPGDYLLVQTVE
                     TVNLPGDIFGYIRPRTTMIRSGIPLETAFISPNYQGRLTVGMKHVGLNEVEIQMGFRI
                     LCIAFFPIDGSAVPYRGVWQGDRVSTDGKEERPF"
     gene            complement(2043..2579)
                     /locus_tag="PLT69_01295"
     CDS             complement(2043..2579)
                     /locus_tag="PLT69_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOJ13117.1"
                     /translation="MNLHRPSWDEYFMMLAKLAASRSTCLSRPTGAVVVKDKQVLATG
                     YNGSLPGQVHCMDEGVCLRRSRQWPEAAKYEMCRSAHAEANAVALAAKKGVSLEGATI
                     YCTLEPCITCSKLIVMSGITRVVYEHPYDSPIPERDRYWKEVLASSGIAVVRLEVGSE
                     TTSYAVEFLGRDTSKRRL"
     gene            3137..4447
                     /locus_tag="PLT69_01300"
     CDS             3137..4447
                     /locus_tag="PLT69_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ13118.1"
                     /translation="MSDFDVVVVGAGYGGMGAAALLAESGLRVLVLEQSESVGGRAWS
                     FTDDEGFTWEYGAHSMRLAEKGIAAELFRRLGDEIVFVPHAKDAGLIYRDRLWDRPEG
                     VIGYLFTPMLPLRSRLALLVLLARIKRTRPEDWYDRTLSDFSRSTGLHSLVEEVLPLL
                     GMAVMCPDPAKVSAGEVIAFVRRMLLAGVSVGEPVGGTAQVFRKLSRHVESSGRIHTG
                     ERATELMVDNGRVAGVRTTRDTYRADTVIFAARLPLLFEIAQPSWFPPELVHYARSIE
                     HSSSLVIDFVTDRPICERKGGILGVDIPVWARFQTNADPSFTPRGLHLSTWGIMLPFG
                     FRGEPEAVDAAERRLRDLVGSLFPLVRDALTRERRLVVEVMNGTVLTPSSSLPHRPPV
                     ACPSPKGLYLAGDTVAGEGCSGDISFSSAMKACDAILEERRRKT"
     gene            4447..5181
                     /locus_tag="PLT69_01305"
     CDS             4447..5181
                     /locus_tag="PLT69_01305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ13119.1"
                     /translation="MSSSCDAVRTSRNAPASPVAALFDMDHTLTWKNAGLTSIQFAYR
                     QGLAPRGLVVLGALRILLYRLSLVNIEQWYERNVSLLRGAALEDMERFAAGWFSAMIK
                     PALYRDAVRLVEEHRRSGHRLVLVSNSPPFLVRVVADGLSIGEIITTILETEDGRLTG
                     RIVKPLCYGTGKRLYVLKWASENGVDLSKSSFYTDSFYDIHLLFDVGRPVAVNPDRRL
                     RAAARRFGWEIMDFERVGAFGPKTPR"
     gene            complement(5669..6739)
                     /locus_tag="PLT69_01310"
     CDS             complement(5669..6739)
                     /locus_tag="PLT69_01310"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HOJ13120.1"
                     /translation="MSTTVAQVKPIVRRPRGYSQAKVLSLDLYRTGGRSGVDLFSIEE
                     RVSYLRKYGDHCMSFSTLQPGKHYFDVPGVGFIAYTWKWGRRFTLADPVCDEKDREMM
                     IREFLKDGVNTAFVQVSEGVARLLHEKFGLYATQFGVEPVVDLSTWDLKGKKKQVMRT
                     SINHAKKEGVVIVENDDPDGCRKLTEEWMKTRTVKRREVIFLIRPMNQPYQEGTRKFC
                     AYHNGELVGFAFFDPIYKDGEVIGYVPNISRFSNKFRYGIFYPLMCHAMEVFKREGVR
                     YLYLGLCPCAVDEQDMPSESKIVKAIVRLLYKYGNWVYSMKGLHFAKSRFEGVEQKTF
                     CAHREALPVKSFLSLFRISRII"
     gene            complement(6778..8319)
                     /locus_tag="PLT69_01315"
     CDS             complement(6778..8319)
                     /locus_tag="PLT69_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOJ13121.1"
                     /translation="MAISPLILKKIYCIGSLKNVEGGVSFTLRNPMKDGFITGVWSVS
                     LNNVEMPLEGVELSANGTRLGASQIDQDRPVPFPMGQDVHFFVGSTSLARNTEHVVKV
                     SCETVPYGVLELEATDQVIETREVRTRIPCDKQNNYDMDIVRARQEFVEEYTGVKLHH
                     IKHHSFDPALTKGNIENFTGVAQVPMGFAGPLKINGEHAKGEFLIPLCTSEGTLVASY
                     NRGMKIINMCGGVTTTVVEDRMQRAPAFAFDSAREARDFVRWIDDHMDDIKTQAESCS
                     KVAKLLNVERYMAGRFVFLRFNYYTGDAAGQNMVSIGTFTACTWILSQVNTIRHFYMD
                     ANLTTDKKASLINTLNTRGKRVVAEIVIPREVLIKNMRTDPETLDHLCRVGALGSFMS
                     GATSNGLHAANALAALFLATGQDVANIAESSTSMIYTEITKEGDLYGSLTLPSLIVAT
                     YGGGTGLPTQRECLEVLGCYGQNKVLKFAEICCAVAMAGEISLAGALSSLDWVTSHER
                     FGKNR"
     gene            complement(8547..>8739)
                     /locus_tag="PLT69_01320"
     CDS             complement(8547..>8739)
                     /locus_tag="PLT69_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13122.1"
                     /translation="TLSRMPVHDRSRGWANTPLGHGREKGYPWVCNVLPTTTTFSDTP
                     TPHNILIINDIEEQVLVNT"
BASE COUNT         1486 a         2798 c         2749 g         1706 t
ORIGIN      
        1 gtcatggtaa gagccgtcat cgttgtagac catgaggggg ctctcgatct cgagacccct
       61 gcccccgccc ttggtcccgc acacgagaca gatctccgag gccgcattcc acgtggtgtg
      121 cacgcacttg aggcgcttgg gttcgatcct caccgcccgc atggcggaga tcaggtcggc
      181 gagcctccag gacgggtaca cgacgtacag tctccccctg tgcctgagca ggtcccgagc
      241 cttcttcagg agcgcttcga ggtcgagcgt tatctcgtgg cgcgctatgg ccttctgcct
      301 gtcggggttc accctgcccg cctggggcgg cctgaaggga gggttcgcca ccaccgcgtc
      361 caccttgtgg cccgtatagt tccgtatgtc cgcgcagacg atctcgacgc tgccttcaag
      421 gccctgtcgc gccacggagc gcctcgacat ctcggcgagc tcctcctgga tctccacgcc
      481 ggtgaccagg agacccttga gggaggccag catcatggac acgaccccgg agcccgagcc
      541 gacctccaga acgcgcgtac caggctcctc ctccacgaac gcggcaagca ggtatgcgtc
      601 cagcgagtac cggtacccgc gttttttctg gaacacgcct atcgagccgc acgccaacac
      661 gtcgagggtc tcgtcgggac ggggctcgaa cgtatccggc atcacccccg tgcgcctccc
      721 tcacgaagcg tggccgcccg gggaagcgcc cgccctctcg agcatgtctt cgaagaggct
      781 gggcgcatgg gtcatcgcgt cattgaacat gaggtatgcg agcctgcgga tctcccactc
      841 tgcggaggtg tcgagcctga gctcgaagaa gtggcgcagg gccctggcgt tcacgtagac
      901 gtacgcggcg gtcgcccagc cgacaggcat gagcctgcgg agttcctggt tgcgcacccc
      961 catggccctg atctcctcgt acacctctat ggcgcgcctg tgctcccgct cgtatgcacg
     1021 gtagatcccc atcacctggt cctccgagtc caggtattcg agcgccgggt agacgaaggt
     1081 gttcttgttc gccttgacgt acctggtgga cctgaacgtc cagccgatgc cgatcctgtg
     1141 ccttgtccac tggcctgcca gcgacttgga gaccccggtc acgaggtaca cgaactgcac
     1201 cgcctccagg caggtgtggt ggccccagct cacgagcttc ctgttgagcc tgaggtcgtc
     1261 ctcgtgcgtc ccctccgact cgtggctctg cctggccgtg tacgcaggca gggtctccgt
     1321 ggtcaccccc tggtcgacaa ggtcctttgt cggcacggtg agacccacga gcctgaggtt
     1381 cggctcctcg ttgcggaaca ggtcttgcct gaggtgtttc catttctcta tgtggttgta
     1441 ttcatgaacg aagtatttca aaacggtctc tcctccttgc cgtcggtgga caccctgtca
     1501 ccctgccaga cccccctgta cggcacggcc gacccgtcga tggggaagaa ggctatgcac
     1561 aggatgcgga atcccatctg tatctccacc tcgttcaggc cgacgtgctt catccccacc
     1621 gtgagcctgc cctggtagtt cggcgagatg aaggcggtct cgagcggtat cccgctccgg
     1681 atcatggtgg ttcgcggcct gatgtagccg aagatgtctc cgggcaggtt caccgtctcg
     1741 acggtctgca ccaggagata gtctcccggg gtcaccctca gcacggcgtc ggggtcttgg
     1801 gcgccgtcca cgagcttgac gatctcgggc gtcaccctga cgtcgcgcat gagccgtgcc
     1861 cgtcccacag gccggtagac cgccccgagc ctcaggtcga ccgtcgtgcc ctcgatgccg
     1921 tccacctggt cctgtccgat atcctcgatg aggggtttcc ggacgatacg tcggctcacg
     1981 gggtcttcga ggtcgacccc ggacctgatc atctcgatga tcctgtccgc tcctatgacc
     2041 acctacagcc tcctcttcga cgtgtccctg ccgaggaatt ccacggcgta cgaggtcgtc
     2101 tcactgccga cctccaggcg caccaccgcg atcccgctgg aagcgagcac ctctttccag
     2161 taccggtccc tctcggggat gggcgagtcg tacgggtgct cgtagaccac cctcgtgatg
     2221 cctgacatca cgatgagctt ggagcaggtg atgcacggtt cgagggtgca gtagatcgtc
     2281 gcaccctcga ggctcacacc cttcttcgcg gccaaggcca ctgcgttggc ctcggcgtgg
     2341 gcggaacggc acatctcgta cttcgccgcc tccggccact gcctagacct cctcaggcac
     2401 accccctcgt ccatgcagtg cacctggccg ggcagggacc cgttgtaccc cgtggccagg
     2461 acctgcttgt ccttcaccac cacggccccc gtcggcctgg agaggcaggt cgaacgggag
     2521 gccgcgagct tcgcgagcat catgaaatat tcgtcccagg agggcctgtg aaggttcatg
     2581 gaaacgtccc catcatcacg aagagccaca ggttgggcac catatagtat gaaacggcga
     2641 ggccgtcaaa tgccatctcc tccctgtcca ggaccgccgc cgaggatgcg cccccgcaag
     2701 ggcaggccgt cgccgcggtt gcatcggggg tcgacgcagc gaagaaccgg tgcatcgtct
     2761 tcgtctccct tccctccatc gtctcgcctg gtccacgaag aacccgccct tgggccggcc
     2821 gcagggcttt ccccatgacc cccggaaacg aggccgcgcg gaccgtgagg cgggccttcc
     2881 ccgggttgcg ggctcgaacg ggcacggctg gatggccttt tcagcggcac tcggacatat
     2941 gctcgagaga ggcttccttg cccccgcgaa cggtccccgg cttggcgaga aaacggcatc
     3001 caccgggccc gtgacaccag tgcgtggacg gccttcccgc ccgggggcca tgcacggaaa
     3061 cgacgtttgc ctggaatgcg gggcgcgttg gtggtaaccg gtaggttacg ggttccaggt
     3121 catcaagggg ggggatatgt ccgacttcga tgtggttgtc gtgggtgcag gttacggcgg
     3181 catgggggcg gcggccctcc tcgccgaatc cgggctcagg gtgctcgttc tcgagcagtc
     3241 ggaaagcgtc gggggaaggg cgtggtcatt caccgacgac gaggggttca cctgggagta
     3301 cggggcacac tcgatgcgcc tggccgagaa ggggatcgcg gcggagctct tcaggcgcct
     3361 tggggacgag atcgtcttcg tgccccacgc gaaagacgcc gggctcatct accgtgacag
     3421 gctgtgggat cgtcccgagg gcgtcatcgg ttacctcttc acgcccatgc tgcccctcag
     3481 gtcgaggctc gcactcctcg tgctgctcgc gaggataaag cgaacgcggc cagaggactg
     3541 gtacgacagg acgctttccg acttttcgag atcgacgggg ctgcacagtc tcgtggaaga
     3601 agtcctgccg cttctcggca tggccgtgat gtgcccggac ccggcgaagg tgagcgcggg
     3661 cgaggtcatc gcgttcgtca ggcgcatgct cctggcgggt gtcagcgtgg gggaacctgt
     3721 gggcgggacc gcccaggttt tccgcaagct ctcccggcac gtggagtcgt cgggaaggat
     3781 ccacaccggc gagagggcga ccgagctcat ggtcgacaac ggcagggttg caggagtgag
     3841 gaccacgcgg gacacctacc gtgcggacac ggtgatcttc gcggcgcgac tccccctcct
     3901 gttcgagatc gcacagccgt cctggttccc gcccgagctc gtccactacg cgaggtcgat
     3961 cgagcacagc tcgagcctcg tgatcgactt cgtgaccgac aggccgatat gcgagagaaa
     4021 gggcggcatc ctcggcgtgg acatccccgt gtgggcgagg ttccagacga acgcggaccc
     4081 gagcttcaca cccaggggac tgcacctctc cacgtggggg atcatgctcc cgttcgggtt
     4141 caggggcgag cccgaggccg tcgatgccgc cgagcggcgg ctcagggacc tcgtcggatc
     4201 cctgtttcca ctcgtgcggg atgccctgac cagggagcga aggctcgtgg tggaggtcat
     4261 gaacgggacg gtcctcacgc cgtcttcgtc cctgccgcac aggcccccag tggcatgccc
     4321 ctcgccgaag gggctctacc tggccggcga cacggtggcc ggagagggct gctcgggcga
     4381 catcagcttc tcctcggcca tgaaggcatg cgacgccatc ctggaggaaa ggagaaggaa
     4441 gacctgatgt cgagttcgtg cgacgccgtg cgtacatccc gcaacgcccc tgcatcaccg
     4501 gttgcagccc tgttcgacat ggaccacacc ctcacctgga agaacgcagg cctcacctcg
     4561 atccagttcg cctacaggca ggggctggcg cccaggggcc tcgtggtcct gggggccctg
     4621 aggattctcc tgtacaggct ttccctcgtg aacatcgagc agtggtacga gcggaacgtg
     4681 tccctgctcc gcggtgcggc gctcgaggac atggaacgct tcgccgcggg gtggttctct
     4741 gccatgatca agcccgccct ctaccgcgac gcggtgcgcc tggtggagga acaccggaga
     4801 tcgggccacc gcctcgtcct ggtctccaac tcgccgccgt tcctcgtgag ggtcgtggcc
     4861 gatgggctct ccatcggaga gatcatcacc acgatcctcg agaccgaaga cgggaggctc
     4921 acaggaagga tcgtcaagcc gttgtgctac ggcacgggca agaggctgta cgtcctgaag
     4981 tgggcttcgg aaaacggcgt cgatctctcg aagagctcct tctacacgga ttccttctac
     5041 gacatccacc tgctcttcga cgtggggcgc ccggtggcgg tgaaccccga ccggaggctt
     5101 cgcgcggctg cccggaggtt cggctgggag atcatggact tcgaaagggt gggagcgttc
     5161 ggtccgaaaa ctccccgctg aacgtttccc gagccgaagc gccgcatccg actacccggg
     5221 ggggacggcg cgggtgacgg ctcgccgggt cgggtgcgta tcggccagaa agctcacgcg
     5281 ccagctcaac agcggcgcgc ctgtggtcgt gatgggttct caggtcggat gcgcatcggc
     5341 caaagatgag cgcctccggc ctgcccccct tctcgggggt tgcccgcccg gagagccgcc
     5401 ttgatccctc gaacaggcgc aaggcagaag gcttaaccgg gtgacaggag attactcgtc
     5461 ggacaagtgc cggcgtcgcc ccgcccttgt cccgggaacg gggcgacgcc ctgaaaccgg
     5521 ggatcgggcc gatcccgaga gtcgagcctc cccgtataaa cggccaccct tccgcccatt
     5581 gcatggtgac agtgagaaca acgcacaagc cgtgcggagg atgcgggtga cgtcctcctt
     5641 gcaagtatcc aagcccccgg caggatcgtc agatgatcct cgagatcctg aagagggaca
     5701 ggaacgattt caccggcaag gcctccctgt gggcacagaa cgtcttttgc tccacgccct
     5761 cgaacctgct cttggcgaag tggaggccct tcatgctgta gacccagttg ccgtacttgt
     5821 agagcagcct gacgatcgcc ttgacgatct tcgactcgga cggcatgtcc tgctcgtcca
     5881 ccgcgcacgg gcacaggccg aggtagagat accgcacacc ttctctcttg aacacctcca
     5941 tggcgtggca catgaggggg tagaagatgc cgtacctgaa cttgttgctg aacctggaga
     6001 tgttcggcac gtagccgatg acctcgccgt ccttgtatat ggggtcgaag aacgcgaacc
     6061 cgacgagctc gccgttgtgg tacgcgcaga acttcctcgt gccttcctga tacggctggt
     6121 tcatggggcg gatgaggaag atcacctcgc gcctcttgac cgtgcgggtc ttcatccact
     6181 cctcggtgag cttgcggcac ccgtccgggt cgtcgttctc cacgatcacc acgccctctt
     6241 tcttggcgtg attgatggag gtgcgcatta cctgcttctt cttccccttg aggtcccacg
     6301 tggagagatc gaccaccggt tcgacaccga actgggtcgc gtagagcccg aacttttcgt
     6361 gaaggagccg ggcgaccccc tcgctgacct gcacgaacgc cgtgttgacg ccgtccttga
     6421 ggaactcgcg gatcatcatc tcgcggtcct tctcgtcgca gacggggtcg gccagggtga
     6481 acctcctgcc ccacttccag gtgtaggcga tgaacccgac gccgggaacg tcgaagtagt
     6541 gcttcccggg ctggagggtg gaaaaggaca tgcagtgatc gccgtatttc ctgaggtacg
     6601 acacccgctc ctcgatggaa aagaggtcaa cgcctgatcg tcccccggtg cgatacagat
     6661 ccagagagag gaccttggct tgcgagtatc ccctcggcct acgtacaatg ggttttacct
     6721 gggcaaccgt cgtactcatg cgtgcacctc tctttctcgc ctcgtccctg acgaccccta
     6781 acggttcttg ccgaaacgct cgtggctcgt cacccagtca agggacgaga gcgcgcctgc
     6841 cagcgatatc tcgccggcca tggctaccgc gcagcagatt tccgcgaatt tcaagacctt
     6901 gttctggccg tagcagccga gcacctccag gcactcgcgc tgggtgggaa ggcccgtgcc
     6961 cccgccgtac gtggccacga tcagggaagg cagcgtaagg gagccgtaga ggtccccttc
     7021 cttggtgatc tcggtgtaga tcatgctcgt ggaggactcg gcgatgttgg ccacgtcctg
     7081 gccggtcgcg aggaacagtg ctgccagtgc gttcgcggca tggagcccgt tgctcgtggc
     7141 gccggacatg aacgagccga gcgcccccac cctgcagagg tggtccaggg tctcgggatc
     7201 ggtcctcatg ttcttgatga ggacctcgcg agggatgacg atctcggcca ccacgcgctt
     7261 gcccctcgtg ttgagggtgt tgatcaggga cgccttcttg tccgtggtga ggttggcgtc
     7321 catgtagaag tgcctgatcg tgttcacctg agagaggatc caggtgcagg ccgtgaaggt
     7381 gccgatgctc accatgttct gccccgcggc gtcgcccgtg tagtagttga acctcaggaa
     7441 cacgaacctg cccgccatgt agcgttcgac gttgaggagc ttcgcgacct tcgagcagga
     7501 ctccgcctgg gtcttgatgt catccatgtg gtcgtcgatc cagcggacga agtcccgtgc
     7561 ttccctggcc gagtcgaagg cgaaggccgg ggcgcgctgc atcctgtctt ccaccaccgt
     7621 cgtcgtgacg ccgccgcaca tgtttatgat cttcatgccg cggttgtagg acgcgacgag
     7681 cgtcccctcg ctcgtgcaca gcgggataag gaattcgcct ttggcgtgct ctccgttgat
     7741 cttcagcggc ccggcgaagc ccatggggac ctgtgcgacg ccggtgaagt tctcgatgtt
     7801 gcccttggtg agagcggggt cgaaggagtg atgcttgatg tggtggagct tcacccccgt
     7861 gtattcctcc acgaactcct ggcgggcccg gacgatgtcc atgtcgtagt tgttctgctt
     7921 gtcgcagggg atcctcgtgc ggacctcgcg ggtctcgatc acctggtcag tggcctcgag
     7981 ctccaggacc ccgtagggga cggtctcgca ggagaccttc acgacatgtt cggtgttcct
     8041 tgccagggag gtggatccca cgaagaagtg gacatcctgg cccatgggga agggcaccgg
     8101 cctgtcctgg tcgatctgag aggcaccgag cctggtgccg ttcgcggaga gctcgacgcc
     8161 ctcaagcggc atttccacgt tgttcaagga aacagaccac acacccgtga taaaaccgtc
     8221 cttcatgggg ttcctgaggg tgaagctcac ccctccctcc acgttcttca ggctcccgat
     8281 gcagtagatc ttcttgagga tcagggggga aatggccacc tgcggctttg actccgtcgc
     8341 cttgctgctg atggaaacga gcctggactc tgtcgtgggg tttctcatcg tcgtcttctc
     8401 cctcatcatg gaatcaggat ctcatcttct tgcccgatca tccaaaagcc ctgggaccta
     8461 tcggcagtac ccttcatgcg tggggtataa tcacgatgcg tgccaagaca cgacggaaac
     8521 agtacgaaac atccctcagg tgagtatcag gtattaacta gtacctgctc ttctatgtcg
     8581 tttattatca atatgttatg cggcgtgggg gtatcggaaa acgtggtggt agtgggtaaa
     8641 acattacata cccagggata ccctttttcc cttccgtggc ccaggggggt gttggcccaa
     8701 ccccgtgacc tgtcgtggac aggcatgcgg gataaggtt
//
LOCUS       DAOSCR010000024         8688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5299_length_8688_cov_3.824974, whole genome shotgun sequence.
ACCESSION   DAOSCR010000024 DAOSCR010000000
VERSION     DAOSCR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5299_length_8688_cov_3.824974"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLT69_01325"
     CDS             <1..231
                     /locus_tag="PLT69_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13123.1"
                     /translation="SFGHGLGVMGHEWYPAIVDNDEFRDVVLEENAVEVAFLAMAVPG
                     VCGMRLETPVRVTVHGGEMLCATEPVLTVIDG"
     gene            complement(313..2487)
                     /locus_tag="PLT69_01330"
     CDS             complement(313..2487)
                     /locus_tag="PLT69_01330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOJ13124.1"
                     /translation="MDRQKELYREEAYELLDGLEESLMELEKNPKDLDVVGRVFRAMH
                     TIKGSGAMFGFNDIASFTHTIEAVYDLVRDGKITVDQHLISLTPASCDVIREMLDASR
                     DGTTPSVEKATLLTSAFMGYLPDSSEADTDRQAGSQPGIERQQVGDLVERTWRIRFKP
                     HRNIFSNGTNPALLLRELGSLGTCSIVSHLEDLPDLSLMDPESCYVWWDVVLTTTADY
                     NAIRDVFIFVEDLCELTIEVIDAPGETDDENPLYKRIGEILLERGDITTEAVMEALRS
                     QKRLGEILVETKAIHPSAVQAALVEQEQVRAARRKRMAGDDTTSVRVPSAKLDALVNL
                     VGELVTVQARLSQISSRRNEAELIGVAEEVERLSSELRDITMGIRMLPIGTSFAKFRR
                     LVRDLSTELGKETRFVMEGEETELDKTIIDKLSEPLVHLIRNSMDHGIEPPGTREQNE
                     KARAGTIKLSAIHSGAYVFISVSDDGRGLDAEAIRAKALAKGIIAPDQALSEKELYSL
                     IFEPGFSTVTEVSSVSGRGVGMDVVRSTVDSLGGTVEVESMPGRGTTVTLKLPLTLAI
                     IDGLLVRIDEESYILPLSVVVECVEITRHDQESIPGRHLVKVRNEIIPYIPLRKTFRL
                     DSTPPDIEQVVITEIAGKRVGFVVDKVIGQHQTVIKSMGSMLKRIEGISGATILGDGT
                     VALIIDVNKIVHHDEHVANALGTHEAMTTVTRPVHGPVSSRI"
     gene            complement(2569..2934)
                     /locus_tag="PLT69_01335"
     CDS             complement(2569..2934)
                     /locus_tag="PLT69_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13125.1"
                     /translation="MGKRILSVDDSTSIRQLVQFTLEKEGYEVVSAVDGRDAIQKAKG
                     KHADMVITDLNMPNVDGIELIRSLRSDPDYRFTPIVMLTTESALEKKDAGKNAGATGW
                     IVKPFKPEQLIAVVKRLIG"
     gene            complement(2906..3259)
                     /locus_tag="PLT69_01340"
     CDS             complement(2906..3259)
                     /locus_tag="PLT69_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ13126.1"
                     /translation="METQIVEWKEKDGGKILVLGGRLTIQDAVRMRELLIEAASSGSD
                     VSIDLSGGESLDVACAQVLASAAKSLEGRGRKLCVTGDPSEGVARCLSDMAIDPFHTS
                     GTGGEGNGQTNPERG"
     gene            complement(3282..5126)
                     /locus_tag="PLT69_01345"
     CDS             complement(3282..5126)
                     /locus_tag="PLT69_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13127.1"
                     /translation="MRLGTIIERFRAALGKGDCRHVPEGSDRAIRWAETIEHLVSRLE
                     DVIRSSEGGFVELVEKLREFHARADEMGQRSRSVVEIMSGEVVERTTVDLERILRSLD
                     DHFANPETHTERTRAVITDQQSTMRRLSSTLDDLSQLVVNLSMLGLLTQVENAHLSTM
                     DTGFLSLAHDVKALAVNIRDRTSAIRSRVEDIQKHLADTLETVSQFGKRANTHARGML
                     SQAMDNQRALQAKHDGLVQASRHMEEEIRSNASAITDIVMSLQFHDITRQQIEHVMEV
                     LTHVSSTIRQNGHSLEDTAVFVSQALDLQRAQIVKSKDELVSAVSNVILRLERIAENV
                     KGILYQARSSTMASDASGMTFLEDLESGIMSVIHSITESSADQERFATTVESASETAT
                     HMSSFVKDIELLGLRLQLIALNARIKAATIGSEGAALDTISGSIYELSKNARGVTQEI
                     STMLERLVHVSGAFRDEFSTVQTQKRQTGERLMASIRELVSLLNEIDSSIRASMGEVE
                     SMGESLMHDLRETTSGISLHEETGLVLDEVTSVLAGLIEEAHEINPTGRTVSSPAFLA
                     EFKKLYTMESERRVHQMHLEASQAAPPVSDTTAATSQEGDLGDNVELF"
     gene            complement(5131..6216)
                     /locus_tag="PLT69_01350"
     CDS             complement(5131..6216)
                     /locus_tag="PLT69_01350"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOJ13128.1"
                     /translation="MKKIKVLIVDDSALVRQTLSDILSSDPRIEVMGTAGDPFIAVEK
                     IGREVPDVITLDIEMPRMDGLTFLKKVMSQHPIPVVVCSSLAVKGGETALRALEYGAV
                     DIIHKPAMATKQMIEESRTLICDAVAAAAQARRQRLSPESGLFQPKLSADVILSRGKP
                     VTHLETTEKVVAVGASTGGTEALKEFLCAMPRDCPGIVIVQHMPEGFTTAFSKRLDSL
                     CSITVKEASNNDTVLPGHALIAPGNRHMLLKRSGARYYVEIMDGPLVSRHRPSVDVLF
                     RSAARYAGSNAIGVIMTGMGDDGAKGLLEMKESGAYTIAQNEATCVVYGMPQEAVKLG
                     AVDRILPLEAIPSEIMSVYRRHLTKTG"
     gene            complement(6213..6764)
                     /locus_tag="PLT69_01355"
     CDS             complement(6213..6764)
                     /locus_tag="PLT69_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOJ13129.1"
                     /translation="MRARILPLRKLETVYLRPGEMYFGTDPARVVTVLGSCISIVMYH
                     RPTSTSSMSHALMPERSCSRKRAVAGDAFVFVDSSIAWMLEQFSRMGIDKKDLEVKIF
                     GGSDMFQDARKGNEALAVGKKNVMTALRVLQQHGISPTAWNVGGTKGRKVVFYTDTGD
                     VFTKFVNSRNDVIMGNRKTGKGS"
     gene            complement(6766..7629)
                     /locus_tag="PLT69_01360"
     CDS             complement(6766..7629)
                     /locus_tag="PLT69_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOJ13130.1"
                     /translation="MNALPRTTCTTGTVSLDVFERLSSLIMSELGIKMPPSKKTMLEA
                     RLAKRMRDLNIDDLGEYVRFLFTPEGTSRELPHMLDAVTTNKTDFFREPQHFTYLAEH
                     AVPTLVASTGAGMRRPLMVWSAACSSGEEAYTLAMVLKEYATHHPGFRFMVLGTDVSN
                     RMIETARLAIYQDTRAEPIPLHLREKYLLKSKDRSKKLVRIAPEIRSVVRFRRLNFLE
                     EDYQLREKMDVIFCRNVLIYFERATQEKVLRHLCEHLAHGGYMFTGHSETISGMNLPL
                     RSVSGTVSRRT"
     gene            complement(7668..8186)
                     /locus_tag="PLT69_01365"
     CDS             complement(7668..8186)
                     /locus_tag="PLT69_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ13131.1"
                     /translation="MSVEGITETRQYLSFKLDNEEYALDISKVREVLDMTRITRVPQA
                     PAFMKGVINLRGSVVPVVDLNKKFGIKDTESTVNTRIIIGEVAVDGEDTVLGVLADSV
                     HEVMEMEPENIEPAPKIGMRLNTDFIKGMGKKDDEFIMILDIDRVFSAEELSMMAKNS
                     AVSGEQTLDACA"
     gene            complement(8236..>8688)
                     /locus_tag="PLT69_01370"
     CDS             complement(8236..>8688)
                     /locus_tag="PLT69_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13132.1"
                     /translation="RLLAQIVPDIGRTAQLVQEITAASNEQNTGAEQINAAIQQLNSV
                     VQQNASAAEEMSSTSEELSSQAVQLQETIAFFKIDRNGSGRNAYSSQHVGRPQANAKT
                     KTLKGLHNDKLKTATGAVKSPAAGSGVIIDLGENESSGDAHDADFERY"
BASE COUNT         1427 a         2734 c         2601 g         1926 t
ORIGIN      
        1 tccttcggcc acgggctggg cgtcatgggc cacgagtggt acccggccat cgtggacaac
       61 gacgagtttc gggacgtggt cctggaggag aacgcggtgg aggtggcctt cctcgccatg
      121 gccgtgcccg gggtgtgcgg catgcgcctg gaaaccccgg tgcgcgtcac cgttcacggc
      181 ggcgagatgc tctgcgccac agagccggtc ctcacggtca tcgacgggtg aggatcccgc
      241 cgccgggtct gtctcgagct gttcgtaacg aacgctcgca acccgaccgg cgggaccctt
      301 atgcagggaa cctcatatac gtgaagagac agggccgtgt accgggcgcg ttacggtggt
      361 catggcttcg tgcgtaccca gtgcgttcgc aacgtgctcg tcatgatgaa caatcttgtt
      421 cacatcgatg atgagggcca cggtaccgtc tcccaggatg gtcgctcccg atatcccctc
      481 gatacgcttc agcatcgacc ccatggactt gatgacggtc tggtgttggc cgatcacctt
      541 gtccaccacg aacccgacac gcttgcccgc gatctcggtt atgaccacct gttcgatgtc
      601 gggcggcgtg gagtccaggc ggaacgtctt tctcagcggg atatagggga tgatctcgtt
      661 tctcactttg acgagatggc gtccggggat cgactcctgg tcgtggcggg tgatctccac
      721 gcactccacc acgacgctca gcggcaggat gtaggactcc tcgtctatcc tgaccagcag
      781 gccgtctata atggcaagag tcaggggcag cttgagggtt accgtggtac cacggccggg
      841 catgctctcg acctcgacgg ttccgcccag ggagtcgacg gtgctccgga ccacatccat
      901 cccgacgccc ctgcctgata cgctggagac ctcggtgacc gtgctgaatc ccggctcgaa
      961 gatcagagag tagagttcct tctcgctgag ggcctggtcg ggcgcgatga tccccttggc
     1021 gagggccttc gccctgatgg cctccgcatc gaggcccctc ccgtcgtcgc tgacggagat
     1081 gaagacatac gcccctgaat gtatggcaga gagtttgatc gtcccggccc tggccttctc
     1141 gttctgttcg cgtgtcccgg gcggctcgat gccgtggtcc atgctgttgc ggatgagatg
     1201 cacaaggggt tcggagagct tgtcgatgat ggtcttgtcg agctctgttt cctccccttc
     1261 catgacgaag cgggtttcct tccccagttc cgtcgagaga tccctgacga ggcgtctgaa
     1321 cttggcgaag ctcgtaccga tgggcagcat ccttatcccc atggtgatgt ccctgagctc
     1381 ggagctcaac cgctcgactt cctctgcgac gcctatcaac tccgcctcgt ttctccggga
     1441 ggaaatctgg ctcagccggg cctggacggt gacgagctcg cccacgaggt tcaccagggc
     1501 atcgagtttg gcagagggga ccctgacact cgtcgtgtcg tcacctgcca tgcgcttcct
     1561 gcgcgccgcc ctcacttgtt cctgttcgac cagggcagcc tgtacggccg atggatggat
     1621 cgctttcgtc tcgacgagga tctcgccaag cctcttctgc gacctgaggg cctccatcac
     1681 cgcctcggtc gtgatgtcgc cgcgctccag gaggatctcc ccgatcctct tgtatagggg
     1741 gttctcgtcg tctgtctcgc cgggcgcgtc gatgacctct atggtgagct cgcagaggtc
     1801 ttcaacgaag atgaagacgt ccctgatcgc attgtaatcc gccgtcgtgg tgagcaccac
     1861 gtcccaccag acatagcacg actcgggatc catcaggctg aggtccggga ggtcttcgag
     1921 atggctcacg atcgaacatg ttcccaggct tccgagttct ctgagcagca gtgctgggtt
     1981 cgtcccgttg gagaagatgt ttcggtgcgg tttgaaccgt attctccacg tccgctccac
     2041 aaggtccccg acctgctgtc tctcgatgcc cggctgggaa ccggcctgcc ggtcggtgtc
     2101 ggcctcgctg ctgtccggga gataccccat gaaggcgctc gtgagcagcg tcgccttctc
     2161 tacggaaggt gtcgtcccgt cacgcgatgc gtccaacatc tcccgtatga cgtcgcagga
     2221 tgcgggggtc aggctgatga ggtgctgatc gaccgtgatc ttcccgtccc tgacaaggtc
     2281 gtagaccgct tctatggtat gggtgaacga ggcgatgtcg ttgaacccga acatggcgcc
     2341 cgatcccttg atggtgtgca tcgccctgaa aacccggccg acgacgtcca ggtccttcgg
     2401 gttcttctcg agctccatca aggattcctc gagaccatcc agcagctcgt acgcctcttc
     2461 ccggtagagt tccttctgtc tgtccatgtc cattctcctc gcgggtgcca tcggccctca
     2521 ggcggcgccg tctcccggca cgcccacacc gcctcttccc gcacaccctc accctatcag
     2581 acgcttcacc accgcgatga gctgttccgg cttgaacggc ttcacgatcc atcccgtcgc
     2641 accggcgttc tttcccgcgt ccttcttctc gagggcgctc tccgtcgtga gcatgacgat
     2701 gggggtgaac cggtagtccg ggtcggatct cagagacctg atgagctcga taccgtccac
     2761 gttgggcatg ttgaggtcgg ttatgaccat gtccgcatgc tttcccttcg ccttctgtat
     2821 cgcgtccctg ccgtcgacgg cggacacgac ctcgtacccc tccttctcga gggtgaactg
     2881 aaccagctgc ctgatgctcg tcgagtcatc cacgctcagg attcgtttgc ccattcccct
     2941 cacctcctgt tccgctggta tggaagggat ctatggccat atcgctcagg caccgtgcga
     3001 ccccttcgga tggatctccc gtgacgcaca acttccggcc tctgccttcg aggctcttcg
     3061 cggcgctcgc gaggacctgc gcgcaggcga catcgagaga ctccccgccg gaaaggtcga
     3121 tgctgacgtc gcttcccgat gaggcggcct cgatgagaag ctctctcatc cgcacggcgt
     3181 cctgtattgt cagcctgccg cccaggacga ggatcttccc cccgtctttc tctttccatt
     3241 caacgatttg agtttccatg ctcgcgatcc cctcccgtgt ctcagaatag ttccacgttg
     3301 tcccccagat ccccttcctg tgacgtcgcc gccgtcgtat ccgaaacggg cggtgcggcc
     3361 tgggatgcct cgaggtgcat ctggtggacg cgcctctcgc tctccatggt gtagagcttc
     3421 ttgaactcag cgagaaaggc cggcgaggag actgtgcgcc cagtcgggtt gatctcgtgc
     3481 gcctcctcga tgagcccggc cagaactgat gtgacctcgt cgaggacgag ccctgtctcc
     3541 tcgtgcagcg agatcccgga cgtcgtctcc ctgaggtcgt gcatgagcga ctctcccatc
     3601 gactcgacct cgcccatgga tgcccgtatc gaggaatcga tctcgttgag cagggacacg
     3661 agttcgcgga tgcttgccat gagtcgctcg ccggtctgtc gcttctgggt ctgcacggtg
     3721 gagaactcgt cgcggaatgc gcctgaaacg tggaccaggc gctccagcat cgtcgagatc
     3781 tcctgcgtga cccccctcgc gttcttcgag agctcgtaga tgctgccgga tatggtatcg
     3841 agcgccgccc cctcactgcc gatagtggcg gctttgatgc gggcgttgag cgcgatgagc
     3901 tggagcctca ggccgaggag ctcgatgtcc ttcacgaaag aggacatgtg ggtcgccgtc
     3961 tcgcttgcgg actccactgt cgtggcgaat cgctcctggt cggccgagga ctcggttatg
     4021 gagtgtatga cggacataat gccggactcg aggtcttcga ggaaggtcat ccccgaggca
     4081 tctgacgcca tggtggagct gcgtgcctgg tacaggatgc ccttcacgtt ctcggcgatc
     4141 ctctcgagtc gcaggatgac gttcgaaacc gcgctcacga gctcgtcctt gctcttgacg
     4201 atctgggcac gctgaagatc cagggcttgg cttacgaaga ccgcagtgtc ctccagagaa
     4261 tgcccgttct gccggatcgt ggacgacaca tgggtgagca cctccatgac gtgctctatc
     4321 tgctggcgcg ttatgtcatg gaactgcagc gacatgacga tgtctgtgat ggccgaggcg
     4381 ttggagcgga tctcctcctc catgtgccgg gaggcctgca cgaggccgtc atgctttgcc
     4441 tggagcgccc tctggttgtc catggcctgg gagagcatgc cccgggcgtg cgtgttcgcg
     4501 cgtttcccga actgggagac cgtctcgaga gtgtctgcga ggtgcttctg gatgtcctcg
     4561 acccgtgacc ggatcgccga ggtgcggtcc ctgatgttca cggcaagcgc cttcacgtcg
     4621 tgtgcgaggc tcaggaaacc cgtgtccatt gtcgagagat gcgcgttttc cacctgggtg
     4681 aggaggccca gcatcgagag gttgaccacg agttgggaga ggtcgtccag cgtggaactc
     4741 aagcgcctca tcgtggactg ttgatccgtg atgacggccc tggttcgttc cgtgtgtgtc
     4801 tcggggttgg cgaaatgatc gtcgaggctg cgcaggatcc tctcgagatc cacggtcgta
     4861 cgctcgacga cctcgccgct catgatctcg accacactgc gggatctttg tcccatctca
     4921 tcggcacgtg cgtgaaactc cctgagcttc tccaccagct ccacgaaacc gccctctgaa
     4981 gacctgatga cgtcttcgag ccgtgaaaca aggtgctcga tggtctccgc ccatcggatg
     5041 gcgcgatcgc ttccctccgg aacatggcgg caatcgcctt tgccaagcgc cgccctgaat
     5101 ctctcgatga tcgtgccgag cctcatggcc tcaccctgtc ttcgtcaggt gtcgtcgata
     5161 gacactcatg atctcggaag gaatggcctc gagggggagg atcctgtcca cggcgcccag
     5221 cttgacagcc tcctgcggca tgccgtagac gacgcaggtc gcctcgttct gggctatggt
     5281 gtaggccccc gactccttca tctcgagcag cccctttgcc ccgtcgtcgc ccatgcctgt
     5341 catgatgaca ccgatggcgt tgcttccggc gtacctcgcg gccgaccgga agaggacatc
     5401 caccgatgga cggtgacgag atacgagagg gccgtccatg atctcgacgt agtacctcgc
     5461 cccgctcctc ttgagaagca tgtggcggtt cccgggtgcg atgagggcgt gcccgggcag
     5521 gacggtgtcg ttgttcgacg cctccttcac ggtgatggaa cacagcgagt cgagcctctt
     5581 ggagaacgcc gtggtgaacc cttcgggcat gtgctggacg atgacgatcc ccgggcagtc
     5641 gcgcggcatc gcacacaaga attccttgag agcctcggtt ccccccgtgg atgcgcccac
     5701 ggcgaccacc ttctccgtgg tctcgagatg ggtcaccggc ttgcccctgg agaggatgac
     5761 atccgcgctg agcttgggtt ggaagaggcc gctctcgggc gagagtcttt gcctcctcgc
     5821 ctgcgcggcc gccgccaccg cgtcgcagat gagggtgcgc gactcctcga tcatctgctt
     5881 cgtggccatg gccggtttgt ggatgatatc cacggcaccg tactcgagcg ccctcagggc
     5941 tgtctcgccg cccttgacgg ccagagacga gcagacgacc acgggtatgg gatgctgaga
     6001 catcaccttc ttcaggaacg tgagcccgtc catgcgcggc atctcgatgt cgagggtgat
     6061 cacatccggc acctctcggc cgatcttctc caccgctatg aacgggtctc cggccgtccc
     6121 catcacctcg atccgggggt ccgaggagag gatgtcggaa agcgtctgcc gcaccaaagc
     6181 ggaatcatcg acgatgagca ccttgatctt tttcatgacc ccttgcccgt ttttctgttc
     6241 cccatgatga cgtcattccg ggagttcacg aatttcgtga acacatcccc ggtatccgtg
     6301 tagaagacca ctttccgacc cttggtgccc cccacattcc acgccgtcgg tgaaatcccg
     6361 tgttgttgga ggaccctcag ggcggtcatc acgttcttct tgccgaccgc cagcgcttcg
     6421 ttcccctttc tggcgtcctg gaacatgtca gaaccgccga agatcttcac ctcgaggtct
     6481 ttcttgtcga tgcccatgcg ggagaactgt tcgagcatcc acgcgatcga ggagtccacg
     6541 aagacgaacg cgtcgccggc cacggccctc ttcctcgaac aacttcgctc gggcatgagg
     6601 gcgtgagaca tggagctcgt ggatgtgggc ctgtggtaca tcacgatgga tatgcacgag
     6661 cccagcacgg tgacgacccg tgcggggtct gtgccgaagt acatctcccc cggcctcagg
     6721 tagaccgtct cgagcttgcg tagcggcaag atccttgcac gcacctcagg tcctcctcga
     6781 taccgtgccg gaaacgcttc tcaggggcag gttcatgccg gagatcgtct ccgagtgccc
     6841 ggtgaacatg tacccgccgt gggcgaggtg ctcgcagaga tgccggagaa ccttttcctg
     6901 cgtggccctc tcgaagtaga tgaggacgtt cctgcagaag atcacgtcca tcttttccct
     6961 gagctggtag tcctcctcga ggaagttcag cctcctgaac ctcacgaccg aacggatctc
     7021 cggcgcgata cggacgagct tcttcgaccg gtccttgctc ttgaggagat acttctcccg
     7081 caagtgcaaa gggatgggct ccgcgcgggt gtcctggtaa atggcaaggc gggcggtttc
     7141 gatcatccgg ttcgagacgt ctgtgccgag aaccatgaag cggaagcccg ggtgatgcgt
     7201 tgcgtactcc ttgagaacca tggcgagggt gtacgcctct tcccccgacg aacacgccgc
     7261 cgaccagacc atgagaggcc tcctcatgcc tgcgccggtg gatgcgacga gggtcggcac
     7321 cgcgtgctcg gcgagatacg tgaagtgctg aggctctctg aagaaatccg tcttgttggt
     7381 ggtgaccgcg tccagcatgt gcggcagttc ccggctcgtc ccctcgggtg tgaagagaaa
     7441 acggacatac tcgcccaggt cgtcgatgtt gagatcgcgc atcctctttg cgaggcgcgc
     7501 ctcgagcatg gtcttcttgc tgggcggcat cttgatgccg agctcgctca tgatgagcga
     7561 gctcagcctc tcgaacacgt cgagtgaaac cgtacctgtc gtgcacgtgg ttcgtggaag
     7621 cgcgttcatc ttcgtccttc gggtcgagcg cggtcacctc gagggcttca tgcacaggcg
     7681 tcaagcgttt gttcgccgga gaccgcgctg tttttcgcca tcatggagag ctcttcggcc
     7741 gaaaagaccc tgtcgatatc caggatcatg atgaactcgt cgtccttctt gcccatgccc
     7801 ttgatgaaat cggtgttcag gcgcatgccg atcttgggag cgggctcgat gttctcgggc
     7861 tccatctcca tgacctcgtg caccgagtcc gcaaggacgc cgagcaccgt atcctcgccg
     7921 tcaacggcca cttcgccgat gatgatcctc gtgttcacgg tggactcggt atccttgatg
     7981 ccgaacttct tgttgaggtc cacgacgggt accacggatc ccctcaggtt gatcacgccc
     8041 ttcatgaacg cgggggcctg cggcaccctg gtgatcctcg tcatgtcgag cacttccctc
     8101 accttcgata tgtcgagggc gtactcttcg ttgtccagct tgaaagagag gtactgcctc
     8161 gtttctgtga ttccctcaac gctcatgtgt tcccccttgt cgtatgcttc agcactggtc
     8221 tggcatgccg tggcatcagt acctttcgaa gtccgcgtcg tgggcgtcgc cgctcgattc
     8281 gttctcgccg aggtcgatga tcacacccga gcccgctgca ggactcttga cggcgcctgt
     8341 tgcagtcttg agcttgtcgt tatgaagacc tttgagcgtc ttggtcttcg cgttggcctg
     8401 cggtctgccg acatgctggg aggaataggc gttcctgccc gagccgtttc tgtctatctt
     8461 gaagaaggca atggtttcct ggagctgcac ggcctggctc gacagttctt cggaagtcga
     8521 cgacatctcc tcggcggctg aagcgttctg ctgcacaacg ctgttgagct gctggatcgc
     8581 cgcgttgatc tgctcggcgc ccgtgttctg ctcattgctg gccgcggtta tctcctggac
     8641 gagctgggcc gtcctgccga tgtcagggac gatctgggcg agaagcct
//
LOCUS       DAOSCR010000025         8637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5338_length_8637_cov_3.025985, whole genome shotgun sequence.
ACCESSION   DAOSCR010000025 DAOSCR010000000
VERSION     DAOSCR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5338_length_8637_cov_3.025985"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /gene="hcp"
                     /locus_tag="PLT69_01375"
                     /gene_synonym="priS"
     CDS             complement(<1..1567)
                     /gene="hcp"
                     /locus_tag="PLT69_01375"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOJ13133.1"
                     /translation="MFCFQCQETARNQGCTVRGVCGKTEETANLMDLLIHTLKGASLY
                     AEKAMGKGSFTEAHGRVMADALFSTLTNTNFDAERIHGYIDKALQLREELLRAAGEDG
                     KTVDHDSALWVAGSREERLSKAKDVGVLATADEDIRSLRELLVYGLKGIAAYAHHAFV
                     LGFEDAEIYRFIAQGLAATTKDLPADTLVSLVEKAGDTAVKTMALLDKANTSTYGNPE
                     ITTVGTGVRANPGILVSGHDLKDLEELLEQTKGSGVDVYTHGEMLPAHSYPFFKKYDN
                     LVGNYGGSWWHQREEFETFRGPILMTTNCIVPVKESYQGRIFTTGTAGYPGVKHIPDR
                     SDGKPKDFSEIIALAKTCPAPRELENGTIVGGFAHNQVTALADKVIEAVKTGAIRRFV
                     VMAGCDGRHKEREYFTRVAEALPADTVILTAGCAKYRYNKLVLGDIGGIPRVLDAGQC
                     NDSYSLAVIALKLKDAFGLDDVNELPLSFDIAWYEQKAVAVLLALLSLGFKGIRLGPT
                     LPAFLSLGVGKVLV"
     gene            1881..2210
                     /locus_tag="PLT69_01380"
     CDS             1881..2210
                     /locus_tag="PLT69_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOJ13134.1"
                     /translation="MQFLIIARDAADEHAVERRMQSREMHLKGIERLREEGAVLFGAA
                     ILDEEGVMRGSMLVVDFPDRQSLENYLADEPYVIGRVWERIDITACRVPDLFMEQRKR
                     HMKDAQR"
     gene            2667..4064
                     /gene="purF"
                     /locus_tag="PLT69_01385"
     CDS             2667..4064
                     /gene="purF"
                     /locus_tag="PLT69_01385"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ13135.1"
                     /translation="MHIEDHEGWHEECGIVGIYGDERASEKVYLGLYALQHRGQESAG
                     IASADGTCIACHKGQGLVWSVFSDPRTMPGLKGHAAIGHNRYSTRGVSTIVNAQPIVV
                     ECRQGTMALAHNGTITNASSLRRLLHDTGSIFQTTSDSEIVLHLVARSREDGVIEAAM
                     KAMEKLVGAYCFVLLAPDMLLAARDPYGFRPLSLGRLGDAYVVASETCAFDIMAAEYV
                     RSLEPGEVLVIDRDGPRSLFLPAAPRRAFCIFEFIYFSRPDSLVFGERVDKIRRRLGK
                     RLAEDAPAQAHIVISVPDSGNTAALGYARASETRYEIGLIRNHYVGRTFIAPHQDKRK
                     LDVRVKLNAVEGVVEGRSIVLVDDSIVRGTTMRQIVRLLRDKGASEVHVRISSPPIRH
                     PCFYGIDISRHSELIASEKTVNEVCSFIGADSLGYLSLEGMLSCVEHPEDYCTACFDG
                     TYPTEVPAGFDKFCL"
     gene            4250..5473
                     /locus_tag="PLT69_01390"
     CDS             4250..5473
                     /locus_tag="PLT69_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13136.1"
                     /translation="MGFIGEDLFHFQATIAQSGTTFEDFVRENYVGKWVDEPLYNVLN
                     VRTTFVTAVNNFLVNEGLLNLERVQMSPVTDPLAHDVEHVPTIHYKGMPYVATHSMIY
                     MKFLACFNPRLKGIFIDSPNIRLEIESPQRKQRGKYLIDFSQIDIEMRRNRGVDFETY
                     KNEPEKVKRILREDYEKAIDFFERMIVAAASAVCEKNDEDLKALGVALEVPKRPFPRV
                     RHDEALKKHGKADLDAKAGDEIDAQFFWVTGLMRENYDLIYPYILQDGSRLPLSSYTS
                     DMIYNYDICAKSIVRKTNTYTRAIEILSGAIREWLYEPIICRLIDNRIIPEPPVFENG
                     MLMNIANLDGYGPFLTAVHMTDDKGKPLFPETMGAGIGVERSLYALLKGGKIEKIDDV
                     TFFGKNPDTYPIFLF"
     gene            complement(5779..8433)
                     /locus_tag="PLT69_01395"
     CDS             complement(5779..8433)
                     /locus_tag="PLT69_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ13137.1"
                     /translation="MRKRGYEDRGQGAGPWGLSPGLGGFRLSWKITRDFDRRIRRLAQ
                     PLSASSGGSWSLAAVGGYGRRELCPGSDLDLLVITSGSTSAPVIEELIRTVIYPLWDM
                     GHTTGYVVKTASEVTPHARSDLHFFTSVMDARYLSGDISLFEDATRSTTRVTSPEEAM
                     RLVAFLGEHARSRHAKNDGFPLHEPDVKDGPGGLRDYHGVVWALGTIRFLACRTRRMR
                     LTRGEKARVVMAADRLLLVRHHLHRMAGNKTDRLLMEHHTELARRLGYTDGFPDTAEE
                     SLLKDVTSTMNDILLTSASFLSRIVSELGMAVREAPAGRAGRDLARMETPFGLKVEAE
                     GAASGDPSDEGRGKKNTKNGPDGGKDGVPDAAGGRKEGKRPRPAHPATAGDVLEVFLR
                     VSMTGLAPSAAEKDAAERSMHLLDNPPGDTSISRPFLDTLTGPYPAEALLAMVETGAL
                     ERVIPGFKAIRGRVPPDPIHRRSVDMHSIETVKELKALESCKKAVFSLVDDTECLYLS
                     ALLHDIGKGYGTPHSVTGSSLAGKTAMALGLGRDRADRVAHLVRFHLLLPDTVSRRDL
                     SEEKTILDVARAVMNPGNLAMLYLLCIADSRATSPVSWDPWKESLLGELYEKTLHVLE
                     KGTYRDLKKTALLERRWSRLASGKHGLPAGHVEGRLWSLPQSYVIHTPLSRIVRHLRL
                     ARGISSRSDVALDVETRADRADVTVVARDRHGLFATLTGIFALFHLEILSAKVFTWYD
                     NTAVDTFHVRLPWSGYDEWDRLEAALRAAMEGVIDVEASLGAKRPLATFPRPQPRWRA
                     HVLVDNSSSDFFTTIEVRALSGISTVYRVSRAITALGLNIHRAVLSIQGSLRLDTFSV
                     VDATGEKVTDEVTVERTVRVIEEAAEGG"
     gene            complement(8543..>8637)
                     /locus_tag="PLT69_01400"
     CDS             complement(8543..>8637)
                     /locus_tag="PLT69_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOJ13138.1"
                     /translation="TGKIGDGKIFILPVEDAIRIRTGESGPDAL"
BASE COUNT         1538 a         2724 c         2823 g         1552 t
ORIGIN      
        1 cgacgagcac cttgccgaca ccgagggaga ggaaggccgg gagcgtcggg ccgagcctta
       61 tgcccttgaa gccgaggctc aagagcgcga gcaggaccgc gaccgccttc tgctcgtacc
      121 aggcgatgtc gaaggacaag gggagctcgt tgacgtcatc gagcccgaag gcgtccttca
      181 gcttgagcgc gatgacggcc agcgagtacg agtcgttgca ctggcccgcg tcgagcaccc
      241 tgggaatgcc gcctatgtcg cccaagacga gcttgttgta gcggtacttg gcgcagccgg
      301 ctgtcaggat cacggtgtct gcaggcaggg cctcggccac cctcgtgaag tattctcgct
      361 ccttgtgcct gccgtcgcag ccggccatga ccacgaaacg cctgatggca ccggtcttga
      421 ccgcctcgat caccttgtcc gcaagcgccg tcacctggtt gtgggcgaac ccgccgacga
      481 tggtgccgtt ctccagctcc ctgggggccg ggcaggtctt cgcgagggcg atgatctcgg
      541 agaaatcctt cggcttgccg tccgagcggt cggggatgtg tttcaccccg gggtagccgg
      601 cggtccctgt ggtgaagatg cgaccctggt acgactcctt caccggcacg atgcagttgg
      661 tggtcatgag gatcgggccc ctgaaggtct cgaattcctc gcgctggtgc caccaggagc
      721 cgccgtagtt gccgaccagg ttgtcgtact ttttgaagaa cgggtaggag tgggccggga
      781 gcatctcgcc gtgggtgtac acgtcgaccc cggagccctt ggtctgttcg aggagctcct
      841 cgaggtcctt gaggtcgtgg ccggacacga ggatgccggg gttcgccctc acaccggtgc
      901 ccaccgtggt gatctccggg ttgccgtagg tggacgtgtt ggccttgtcc agcagggcca
      961 tggtcttgac ggccgtatcg cctgccttct cgacgaggga gacgagggtg tcggcgggca
     1021 ggtccttggt cgtcgcggcg aggccctgcg cgatgaaccg gtagatctct gcgtcctcga
     1081 acccgaggac gaaggcgtgg tgcgcgtacg cggcgatgcc cttcaacccg tagaccagga
     1141 gttccctgag cgagcgtatg tcctcgtcag cggtggcgag gacgccgacg tctttggcct
     1201 tcgacagcct ctcctccctc gagccggcca cccacagggc gctgtcgtgg tctaccgtct
     1261 tgccgtcctc tcccgcagcc ctcaggagtt cctccctcag ctgcaaggcc ttgtcgatgt
     1321 agccgtggat gcgttcggcg tcgaagttgg tgttcgtgag cgtggagaac agcgcgtccg
     1381 ccatgactcg cccgtgcgcc tcggtgaacg atcccttgcc cattgccttc tccgcgtaga
     1441 gcgatgcgcc cttgagcgtg tggatgagca ggtccatgag attcgcggtc tcctcggtct
     1501 tgccgcagac ccccctgacg gtgcacccct ggttgcgagc cgtttcctga cactggaaac
     1561 agaacatgtc ctttacctcc ctttcttgaa atggtggccc accatacccc atgcatggta
     1621 aggtctcctt gacctgagtc aaggttcgga gatgacgccc ccatcctgcg gggcatgcct
     1681 gaccgtcgtg ttcgcgccca gggctgccgg gagctctcgc atcacgggag ggtcgcgcat
     1741 ggcgtggatg gtgaagcacg ctcaaaaaaa ggcagagccc gcttttgatg atgaggaaaa
     1801 ggatcgtagg ggaaagggcg cccgcttgag tgctcgagga gaggatgggg ccgatcccaa
     1861 ggagaggggg agacggggat atgcaatttc tcataatcgc gagggacgcc gcggacgaac
     1921 acgccgtcga gcgcaggatg cagtcacgcg agatgcacct gaagggtatt gagagactga
     1981 gggaagaagg ggctgtgctc ttcggcgccg caatcctgga cgaagaggga gtcatgcggg
     2041 gctcgatgct cgtcgtcgac ttcccggaca ggcaatcctt ggagaactac ctcgcggacg
     2101 aaccctacgt gatagggcgc gtctgggaaa ggatagacat cacggcctgc agggtgcccg
     2161 atctcttcat ggagcagcgt aaaagacaca tgaaagatgc acaaaggtga acatcctgcc
     2221 tgaatcggac aaaaatgtgc ctttcgtgcg gggagcctgc tcgaggtgaa gccggggatg
     2281 gagatgatgt tcgggggcgc tcggcagagg aggttcgcct cctcgaggac gggatcatga
     2341 gcggccggaa gcagcgacgg gccggaacga caaggccgcg atacgctccc aaacggcctg
     2401 cggcggcgcg cagccgcgga tgttcggggt gtgaggggga cgcaggtgcc gatgcgccgg
     2461 gggcggccag atcctcatgg tggacgggtc tcttcccggt gtgaccgttc tcggccgtgc
     2521 ctggtcggga tgttcaccaa gattttcgtg tgacagggat ctttccccgg tttgatgcgg
     2581 ccgtgaccct cccgcaccga ctcgtccctt gaccggcgtc gtagcgaggg gtatccatgg
     2641 tgccgaactt gcatagggtg gggatcatgc acatcgaaga ccacgagggc tggcacgagg
     2701 agtgcgggat cgtcggcatc tacggcgacg agcgcgcctc cgagaaggtg tacctcggct
     2761 tgtacgccct ccagcacagg gggcaggaga gcgcgggcat cgcctcggcc gacgggacct
     2821 gcatcgcctg ccacaagggc cagggcctcg tctggtcggt cttctccgac ccccggacga
     2881 tgcccgggct caagggacat gctgccatcg gccacaacag gtactccacc cggggggtga
     2941 gcacgatcgt caacgcccaa cccatcgtcg tcgagtgccg gcagggcacg atggcgctcg
     3001 cgcacaacgg caccataacc aacgcatcgt ccctgagaag actcttgcac gacaccgggt
     3061 ccatattcca gacgacctct gacagcgaga tcgtcctcca cctcgtcgcg cgctcgaggg
     3121 aggacggggt gatcgaggcc gcaatgaagg ccatggagaa actcgtgggt gcgtactgtt
     3181 tcgtcctgct ggcacctgac atgctccttg cggccaggga cccgtacggg ttcaggcccc
     3241 tcagcctggg caggctggga gacgcgtacg tggtggcttc agagacctgc gccttcgata
     3301 tcatggccgc cgagtacgtg cggtcgctgg agcccggcga ggtcctcgtg atagaccgcg
     3361 acgggccgag gtccctgttc ctcccggctg cgcccaggcg tgccttctgc atcttcgagt
     3421 tcatctactt ctcccggccg gacagcctcg tcttcggcga gagggtcgac aagatacgca
     3481 ggcgcttggg caagaggctc gcggaagacg cgccggcaca ggcccacatc gtgatctcgg
     3541 tgcctgactc cggcaacacc gcggccctgg ggtacgcccg ggcatcggag acccgctacg
     3601 agataggcct catcaggaac cactacgtcg gccgcacctt catcgccccg caccaggaca
     3661 agcgcaagct cgacgtcagg gtcaagctca acgcggtgga gggggtcgtc gagggcagat
     3721 cgatcgtgct cgtggacgac tccatcgtga gggggaccac catgagacag atcgtgcggc
     3781 tcctcaggga caagggcgca agtgaggtgc acgtgaggat cagctcccca cccatcaggc
     3841 acccctgttt ctacgggata gacatctcca ggcactccga gctcatcgcc tcggagaaga
     3901 cggtgaacga agtgtgctcg ttcatcgggg ctgactcgct cggctatctc agcctcgagg
     3961 gcatgctctc gtgcgtggag cacccggagg actattgcac ggcgtgcttt gacgggacct
     4021 acccgacgga agtgcccgcg ggcttcgaca agttctgctt gtgatcgtga gccgcgtttt
     4081 gctccgggag ggaaagggcc ggtcgggtca ggcgcacgcg gcaaggtgaa cgggatcccg
     4141 ccagggtcct gagggtggca ctcatattgc aaaagaagtg tcgtcgacga gaagcccccg
     4201 gggggcatga gcccaaccgg agggtacaag accaaggagg aggcgcgtta tgggtttcat
     4261 cggagaagat ctgttccatt tccaggccac catcgcacag tcagggacca ccttcgaaga
     4321 cttcgtccgc gagaattacg tgggcaaatg ggtcgacgag ccgctctaca acgtgctcaa
     4381 cgtgcgcacc acgttcgtca ccgcggtgaa caactttctc gtcaacgagg gcctgctgaa
     4441 cctggagcgc gtgcagatga gccccgtgac cgaccctttg gcccacgacg tggagcacgt
     4501 gcccaccatc cactacaagg gcatgccgta cgtggcgacg cactcgatga tctacatgaa
     4561 gttcctcgcc tgcttcaacc ccaggctgaa ggggatcttc atcgactcgc cgaacataag
     4621 gctcgagata gagagcccgc agcgcaagca gcggggcaag tacctcatcg acttctcgca
     4681 gatcgacatc gagatgagga ggaacagggg cgtcgacttc gagacctaca agaacgaacc
     4741 ggaaaaggtg aagcgtatac tcagggaaga ctacgagaag gcgatcgatt tcttcgagcg
     4801 catgatcgtg gcggccgcaa gcgccgtgtg cgagaagaac gatgaagacc tcaaggcgct
     4861 cggggtggcg ctcgaggttc ccaagcgccc cttcccgagg gtcaggcacg acgaggcctt
     4921 gaagaagcac ggcaaggcgg acctcgacgc caaggcgggc gacgagatag acgcccagtt
     4981 cttctgggtg acgggcctca tgcgcgagaa ctacgacctc atctacccgt atatccttca
     5041 agacgggagc aggctgccgc tgtcgtcata cacctcggac atgatctaca actacgacat
     5101 atgcgccaag tcgatcgtga gaaagaccaa cacgtatacc cgggcgatcg agatcctctc
     5161 cggcgccatc agggagtggc tctacgaacc catcatctgc cgcctcatcg acaaccggat
     5221 catccctgag cccccggttt tcgagaacgg gatgctcatg aacatcgcca acctggacgg
     5281 ctacggcccg ttcctcaccg ccgtgcacat gacggacgac aaggggaagc ctctcttccc
     5341 cgagaccatg ggcgcgggca tcggcgtcga gcgcagcctg tacgccctgc tcaagggtgg
     5401 gaagatagag aagatagacg acgtgacctt cttcggcaag aacccggaca cgtacccgat
     5461 cttcctcttc tagaaacgaa gtccggccgg cagaggcgtg tgccggccgg actccgacct
     5521 ctcgtacgcc cggcccctta caggcctgcc gcatacccgc ccaggtgtgt accgggcgcc
     5581 tcagcttcgg cgcgccgccc atgtgcaccc aggagccggc tgtctgcgcg tcttcgcacg
     5641 aaggggaagt cgggccaggc gtgggccgga tacggccgtg acccgcccct ggccgaacga
     5701 gggtccggct cccgccggga cagcccggga acctgccttg gcgcatggga tgcgccgtcg
     5761 ccgatgacga gggcccgttc agcctccctc ggcggcctcc tctatcaccc gcaccgtcct
     5821 ctccacggtc acctcgtccg tcaccttctc ccccgtcgcg tcgacgaccg agaaggtatc
     5881 caggcgcagc gatccctgga tggagaggac cgccctgtgg atattcagac ccagggccgt
     5941 gatggctctg gagacacggt acacggtgga tatcccggag agcgccctga cctcgatggt
     6001 cgtgaagaaa tcggagctcg agttgtcgac gaggacgtgc gccctccacc ttggttgagg
     6061 cctggggaag gtcgccagcg gtctcttcgc tcccagggat gcctccacgt cgatgacccc
     6121 ctccatcgcg gcgcgcaagg cggcctccag gcggtcccac tcgtcgtacc cggaccaggg
     6181 cagcctcacg tggaaggtgt cgacagccgt gttgtcgtac cacgtgaaga ccttcgcgga
     6241 gaggatctcc aggtgaaaaa gggcgaatat cccggtgagc gtcgcgaaca ggccgtgacg
     6301 atcccgcgcc accaccgtca cgtcggcccg gtcggcgcgg gtctcgacgt cgagggccac
     6361 atcggagcgt gagctgatcc ccctggcgag cctcaggtgc cgcactatgc gcgacagggg
     6421 ggtgtggatc acgtagctct ggggcaggga ccacagcctg ccttcgacat gtccagccgg
     6481 gagaccgtgc tttccggatg cgagcctcga ccatctcctt tcgaggagtg cggtcttctt
     6541 gaggtccctg taggtgccct tctccaggac gtgcagggtc ttctcgtaca gctcgccgag
     6601 gaggctctcc ttccaggggt cccacgagac aggggacgtc gcacgggagt ccgctatgca
     6661 caggagatag agcatggcga ggttccccgg gttcatgacg gcgcgggcca catcgaggat
     6721 ggtcttttcc tccgagagat ccctcctgct caccgtgtcc ggcaggagga ggtggaacct
     6781 gacgaggtgg gccaccctgt cggccctgtc ccggcccagg cccagggcca tggccgtctt
     6841 tcccgccaag ctcgagcccg tgacggagtg cggggtgccg tagcccttcc cgatatcgtg
     6901 caggagtgcc gagaggtaca gacactccgt gtcgtccacc agggagaaga cggccttctt
     6961 gcagctctcg agggccttga gttccttcac cgtctctatg ctgtgcatgt cgacggaccg
     7021 cctgtgaatg ggatcgggcg gtacccttcc ccgtatggcc ttgaaccccg ggatgacgcg
     7081 ctccagcgcc cctgtctcga ccatggccag gagggcttcc gccgggtaag ggcctgtcag
     7141 cgtgtccagg aacggccgcg atatcgaggt gtcgcccggc gggttgtcga ggaggtgcat
     7201 ggacctctct gcggcgtcct tctcggccgc ggacggtgcg agacccgtca tcgagaccct
     7261 caggaagacc tcgaggacat cccccgccgt cgcgggatgc gccgggcgag gtcgcttgcc
     7321 ctccttcctc ccgcctgcgg cgtcggggac gccgtctttc cctccgtccg gcccgttctt
     7381 ggtgttcttc tttccgcgcc cctcgtcgct cggatccccg gatgcggcgc cttcggcctc
     7441 aaccttcagg ccgaagggcg tctccatcct tgcgaggtct cgtcctgcac gacccgcggg
     7501 tgcttcacga accgccatgc ccagttcgct gacgatccgc gagaggaatg acgcagaggt
     7561 gagcaggatg tcgttcatcg ttgaggtcac gtctttcagg agcgattcct cggccgtatc
     7621 agggaacccg tccgtgtacc cgagtcgcct ggccagttcc gtgtggtgtt ccatgaggag
     7681 cctgtcggtc ttgttccccg ccatccggtg gaggtggtgg cgaacgagga gcagcctgtc
     7741 tgcagccatg accaccctgg ctttctctcc ccgggtcagg cgcatccggc gtgtacggca
     7801 tgcaaggaag cggatcgtcc cgagggccca tacgacgccg tggtaatctc tcagcccccc
     7861 ggggccgtcc ttgacgtcgg gttcgtgaag ggggaacccg tcgttcttcg cgtgcctcga
     7921 tcgggcatgt tctccaagga acgctacgag gcgcatcgcc tcttccggcg aggtgaccct
     7981 cgttgtggac cgggtggcgt cttcgaagag ggagatgtcg cccgacaggt agcgggcatc
     8041 catcaccgag gtgaagaaat ggagatcgga gcgcgcatgc ggggtgactt ccgaggcggt
     8101 cttcaccacg tagcccgtgg tgtgtcccat gtcccacagc gggtatatga ccgtccgtat
     8161 gagctcttcg atcacggggg ccgatgtgga tccggacgta atgaccagga gatccaggtc
     8221 tgaacccgga cagagctctc tcctgccgta cccgcccacc gcggccaggc tccaggagcc
     8281 accggatgag gcggacaaag gctgagcgag tcttcggata cgccggtcga aatccctcgt
     8341 gatcttccac gagagcctga atcccccgag gccaggggac aggccccacg ggcctgcccc
     8401 ctggcctcgg tcttcgtacc ctctctttct catggaagtc tcttcgtgca cggtgccgtc
     8461 cggtcgtccg caggtggcgg cccacaggtg tcccgccatg gaggacgccc aagcggatgc
     8521 cgagaggggc ccggcgctcg tctcagaggg cgtcggggcc cgactccccg gtccgtatcc
     8581 ggatggcgtc ctcgacgggg aggatgaaga tcttcccgtc gccaatcttg ccggtct
//
LOCUS       DAOSCR010000026         8633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5345_length_8633_cov_3.279669, whole genome shotgun sequence.
ACCESSION   DAOSCR010000026 DAOSCR010000000
VERSION     DAOSCR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5345_length_8633_cov_3.279669"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PLT69_01405"
     CDS             <1..1242
                     /locus_tag="PLT69_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13139.1"
                     /translation="TVEHIVVLSHTGKKPASNLPNLIMYDDLIAGFPEEYDFPADLSE
                     RDAAMICYTSATTGDPKGVVYSHRAIVLHSYAVAVTFGASEADCILHIVPMFHANAWC
                     GPFAGVGAGCKQVLPGREVINMEKLCRTIAEEKVTFTAGVPTIWMLLYDYLEKGGWHD
                     FSSLKTIASGGAAIPRQLLIDLNEKYGFPIKQAYGSTETTPLVSAAIPKSYMADWPAE
                     KLYDVKTSAGLIAMGLEMKIVNDSGEEVKMDGKEWGEICLRGPWIADEYYKDPERSRE
                     TFVDGWYHTGDIATIDEEGYIRLVDRKKDLVKSAGEWISSVDLENLIMAHPKVLEAAI
                     IGVPHPKWQERPLACIVCSPGQSLTADEIKDFLKDKVKASWWIPDEIIFLPELPKTSV
                     GKFNKRELRRLYAEGLLAPKS"
     gene            1261..2412
                     /locus_tag="PLT69_01410"
     CDS             1261..2412
                     /locus_tag="PLT69_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13140.1"
                     /translation="MSLLDRYYTEEHHIFRESVRRYFEREVTPNGDAWEREGIVPKEA
                     WRKFGEQGFLCPWLPEEYGGSGADFLYSFILAEEMAKTHVGGFLVHLHSDIVVPYLYS
                     YGTEEQKKRWLPGCVTGEIITAVAMTEPGAGSDLAAIRTTAVKDGDSYVINGQKTFIS
                     NGINCDLVIVAARTDMNAASPYEGMSLIVVEDGTPGFEKGRKLEKVGLHSQDTAEMAF
                     VDCRVPASNQLGQEGHGFYYLMDKLQQERLVCAMGSQVAAEEALRLTIEYTKTREAFG
                     RPISRFQYISFELAKLATEVEVGRAFMESLLIDHLEGRKDVKKASMAKYWICEMLNRV
                     VAKCVQFHGGYGYMEEYPIARMFRDARVQTIYAGTSEIMLLIISRQLGL"
     gene            2520..3845
                     /locus_tag="PLT69_01415"
     CDS             2520..3845
                     /locus_tag="PLT69_01415"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOJ13141.1"
                     /translation="MAKKGDNTRQLRSVVFVDGCRVPFQRSGTGYYDLMAWELGRFAV
                     KGLLERTGLDPESIDHVIMGSVATDIATTNVAREVALGAGIPKTVPAHTCTIACISAN
                     TAVINGANLIATGNADVVVAGGVETFSDTDIRVSKRFRRFILDVTMYHKPKNLLGLVK
                     RLRDMSPLDFLVPERPAVSEYSTGLLMGQTAEILAKRLGISRAEQDEYAVLSHQRAVT
                     AQEQGWFGREVTPVVVPGKDKVITQDNGPRKDTTVERASRLKGAFDRRYGTVTAANSS
                     FLTDGASAVLMMSEEKARAMGIKPKAYLVSYAFSGQDLVEELLLGPAFTIPMALGKAG
                     LKLKDIGVFEIHEAFAAQIVGNLRCLASRQFAREKLGLDDAVGVIDIDRLNRYGGSLS
                     LGHPFGATGGRLVTTCMNRMQDEDARFGVVAGCGAGALGNAIVLENANA"
     gene            3858..6032
                     /locus_tag="PLT69_01420"
     CDS             3858..6032
                     /locus_tag="PLT69_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOJ13142.1"
                     /translation="MNNLTYEKHEGGVALITIDCKDSKVNKVSSGLLAEISSLLDDMG
                     ADKEIRALLVVSGKDDNFVVGADVDELNAMKTRQEVITYISKAHEVLRRIEQLPFPVV
                     CAIHGNCLGGGLELALVADYRMASDSPKTILGLPEVQLGLIPAAGGTQRLVRLIGLSE
                     ALPLMLTGRNLRPKKALKLGLVDEVVSAYGLKEAGLAKARQLASGAKSPMRAKKRLKD
                     RVLDATVKRFIDETNAGRRIVFKKAREQVLRQTMGLYPAPLAIIDSVEYGLANGIDKG
                     LKRDIEVFADLVMDDKSKAFRSLFEGMTALKKNPLKDQAQKVRKLAVLGAGLMGHGIV
                     SVSTGICDTVLLKDVSLDAAAKGMREVYRGLDKRARSGAISRFERDLHYGKVVPTDDY
                     SLFRNVDLVIEAVFEDLALKRKVLAEVEAAASERTIFASNTSALPITLIAEGCSRPAN
                     VIGMHYFSPVPKMPLLEIITTEHTADWVKATALEFGIQQGKTCIVVKDCPGFYTTRIL
                     IPMLNESMRLLEEGAEIRAVDTAMRRFGFPVGPVTLVDELGIDVAAHVAKGEVKKLFE
                     ARGIEASNGYTKLFEKGFKGRKNRKGFYVYDEQKKGLLPLGRKKGPKQVNTEVYEILG
                     GQRKAFDESEIQERLALAMINEAALCLEEGVLSCARDGDIGAVFGLGFPPFTGGPFRY
                     IDTVGASSVVDRLKRLEDRFGALYTPARILLEAAGSGKKFRG"
     gene            complement(6385..7146)
                     /locus_tag="PLT69_01425"
     CDS             complement(6385..7146)
                     /locus_tag="PLT69_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ13143.1"
                     /translation="MKGSRGKRAVKRFISPIKKAPAIPTLVAEQILELIRKGRLKPGD
                     RLPSEHEMTMRFRISRISLREAMKLLEARGYIESRRRRGKFIRIPQDAPKSTIEDLLA
                     ADQQKIWELLCVRRILDSEAVRLACAHATARDVQRLRKILDRALAIGPERVLDDIEEG
                     GRLYTEFFDTILASTRNATFAHIRRSINTILVGAFPYSRKKLSTIQGSSRVIVSQLHE
                     IVEGIASKDASRACRAVEEHIDYLKRSLKKAMQAS"
     gene            complement(7701..>8633)
                     /locus_tag="PLT69_01430"
     CDS             complement(7701..>8633)
                     /locus_tag="PLT69_01430"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOJ13144.1"
                     /translation="VKGKFGARAPEVAKVVQEWASQRGETVVGHGAAAANRLGLTTQV
                     PVRQVFLTSGRSRKLKLGAQTAPEDIVAVGERVAVKVLAVEADPNTGRRKISLSMKQV
                     GEDPWNTVTERFRPMQKLRGRVTRTADFGVFVEIAPGVEGLVHVSEMSYTTRVKRPSD
                     LVKAGDEVDVMIKEIQPEQRRISLSMKEAEGDPWIELDRRYPVGRVVEGVVEKKERFG
                     VFVRIEPGVTGLLPRSVIDRSALASSVDKLREGDPVTVTIERVDMEKRRISLSPADTC
                     EEGDWRPFSTQPQGRLGTLGDKLLTALEKGKKKD"
BASE COUNT         1602 a         2673 c         2895 g         1463 t
ORIGIN      
        1 accgtggagc acatcgtggt cctgtcgcac acggggaaga aaccggcgtc caaccttccg
       61 aacctgatca tgtacgacga cctgatagcg gggttccccg aggagtacga cttcccggcg
      121 gatctctccg agagggatgc ggccatgatc tgctacacct cggccacgac cggcgacccc
      181 aagggcgtcg tctactccca ccgggcgatc gtgctgcaca gctacgcggt cgcggtcacc
      241 ttcggcgcca gcgaggcgga ctgcatcctc cacatcgtgc ccatgttcca tgccaacgcc
      301 tggtgcgggc ccttcgccgg ggtcggggcc gggtgcaagc aggtgctccc gggcagggag
      361 gtcatcaaca tggaaaagct ctgcaggacc atcgccgagg agaaggtcac cttcacggcc
      421 ggcgtcccca ccatctggat gctcctgtac gactacctgg agaagggggg ctggcacgac
      481 ttcagctcgc tcaagaccat cgcaagcggg ggggccgcca tacccaggca gctcctcatc
      541 gacctgaacg agaagtacgg gttccccata aagcaggcgt acggctcgac ggagaccacc
      601 cccctggttt cagccgccat tcccaagagc tacatggccg actggccggc ggagaagctc
      661 tacgacgtca agacgagcgc aggcctgatc gccatgggtc tcgagatgaa gatcgtgaac
      721 gactccggcg aggaagtgaa gatggacggc aaggaatggg gcgagatctg cctgaggggg
      781 ccgtggatag ccgacgagta ctacaaggac cccgagaggt cgagggagac cttcgtcgac
      841 gggtggtatc acacggggga catcgccacc atcgacgagg aggggtacat caggctcgtc
      901 gacaggaaga aggacctggt caagagcgca ggggaatgga tctcctcggt cgatctcgag
      961 aacctcatca tggcccaccc caaggtgctc gaggctgcca tcatcggcgt tccgcatccc
     1021 aagtggcagg agcggccgct cgcatgcatc gtgtgctcgc cgggccagag tctgaccgcg
     1081 gacgagatca aggacttcct caaggacaag gtgaaggcct cgtggtggat ccccgatgag
     1141 atcatcttcc tccccgagct gcccaagacg agcgtcggca agttcaacaa gcgggagctc
     1201 aggaggttgt acgcagaggg tctccttgcg cccaagagct gatttcgagg aggagaagac
     1261 atgtcgcttc tggacaggta ctacacagag gaacaccaca tttttagaga atccgtgagg
     1321 cgctacttcg aacgcgaggt caccccgaac ggggatgcct gggaacggga ggggatcgtt
     1381 ccgaaggagg cgtggcgcaa gttcggggag cagggcttcc tgtgcccatg gcttcccgag
     1441 gagtacgggg ggtcgggggc cgacttcctc tactcgttca tcctggccga ggagatggcc
     1501 aagacccacg tgggcgggtt cctggtgcac ctccactcgg acatcgtggt gccgtacctc
     1561 tatagctacg ggaccgagga gcagaagaag aggtggctgc cgggctgcgt caccggggag
     1621 atcataaccg ccgtcgccat gaccgagcct ggcgcgggtt ccgacctggc cgccatccgc
     1681 accacggcgg tcaaggacgg tgactcgtac gtcatcaacg ggcagaagac cttcatatcc
     1741 aacgggatca actgcgacct cgtgatcgtt gcggcacgaa cggacatgaa cgcggcctcc
     1801 ccttacgagg gcatgtccct catcgtcgtg gaggacggta ccccggggtt cgagaagggg
     1861 aggaagctgg aaaaggtggg gctccactcg caagacacgg ccgagatggc ctttgtggat
     1921 tgcagggttc ccgcctcgaa ccagctcggg caggagggcc acgggttcta ctacctcatg
     1981 gacaagctcc agcaggaacg cctcgtctgc gccatggggt cgcaggtggc agcggaggag
     2041 gcgttgcgcc tcaccatcga gtacacgaag acgcgcgagg cattcggcag gcccataagc
     2101 aggttccagt acatctcgtt cgagctcgcc aagttggcca ccgaggtgga ggtgggcagg
     2161 gcgttcatgg agagcctcct catagaccac cttgagggcc ggaaagacgt caagaaggcg
     2221 tccatggcca agtactggat ctgcgagatg ctcaaccgcg tcgtcgcgaa gtgcgtccag
     2281 ttccacggcg ggtacgggta catggaggag tacccgatcg cgcgcatgtt ccgtgacgcg
     2341 cgggtgcaga ccatctacgc gggaacgtcg gagatcatgc tcctcatcat ctcgaggcag
     2401 ctgggactct gaggacgcgt cgacgggccc gcccccggca tggaacacgg aagcaagcgg
     2461 caagggcggg gcgaccaggc tcaagcgaaa cgacaagaca tccattcgtt ggaggggaca
     2521 tggccaagaa aggtgacaac acgagacagc tcaggtccgt ggttttcgtc gacgggtgcc
     2581 gggtgccgtt ccagaggtcg gggacggggt actacgacct catggcgtgg gagctcggca
     2641 ggtttgcggt gaagggcctc ctggagagga cgggcttgga cccggagtcc atcgatcacg
     2701 tgatcatggg gagcgtcgcg accgatatcg cgaccacgaa cgtggcgagg gaggtggcgc
     2761 tcggcgcggg catacccaag accgtccccg cgcatacctg cacgatcgcg tgcatatccg
     2821 ccaacacggc cgtgataaac ggggcgaacc tcatcgccac cgggaacgcg gacgtggtgg
     2881 tcgccggggg cgtggagacc ttctccgata cggacatacg ggtttccaag aggttccgca
     2941 ggttcatcct cgacgtcacc atgtaccaca agccgaagaa cctgctcggg ctcgtcaaga
     3001 gactcaggga catgagcccg ctcgatttcc tcgtccctga gcggcctgcg gtttccgagt
     3061 attccaccgg cctcctcatg ggccagaccg ccgagatcct cgccaagagg ctcggcatat
     3121 cgcgcgccga gcaggacgag tacgcggtgc tctcgcacca gagggcggtg acggcgcagg
     3181 agcaggggtg gttcggccgc gaggtgacgc cggtcgtggt gcccgggaag gacaaggtca
     3241 taacccagga caacgggccg aggaaggata ccaccgtcga gcgggcctcg cgcctcaagg
     3301 gcgccttcga ccgcaggtac gggacggtca ccgcggccaa ctcgtctttc ctgaccgacg
     3361 gggcctccgc cgtcctcatg atgagcgagg agaaggcgag ggccatgggg atcaaaccca
     3421 aggcatacct cgtctcgtac gcattctccg ggcaggacct cgtggaggag ctcctcctcg
     3481 gtccggcttt caccataccc atggccctgg ggaaggcggg cctcaagctg aaggatatcg
     3541 gtgtgttcga gatccacgag gccttcgcag cgcagatcgt cgggaacctc aggtgcctgg
     3601 cctcgcgcca gttcgcgagg gagaaactgg ggctggacga tgccgtgggc gtcatagaca
     3661 tcgacaggct gaaccgttac ggcgggtcgc tttccctggg ccacccgttc ggcgctacgg
     3721 gcggcaggct cgtcaccacg tgcatgaacc gcatgcagga cgaggacgca cgtttcggcg
     3781 tcgtcgccgg gtgcggagcc ggggccttgg gcaacgccat cgtactcgag aacgccaatg
     3841 cctgacgggg ggagaccatg aacaacctga cctacgaaaa gcacgagggc ggcgtcgccc
     3901 tgatcaccat agactgcaag gactccaagg tgaacaaggt ctcgagcggg ctgctcgccg
     3961 agatctccag cctcctggac gacatgggtg cagacaagga aataagggcc ctgctcgttg
     4021 tgagcgggaa ggacgacaac ttcgtggtcg gcgcggacgt ggacgagctc aacgccatga
     4081 agaccaggca agaggtcata acctacatct cgaaggcgca cgaggtcctg cgccggatcg
     4141 agcagctccc cttccccgtg gtgtgcgcca tccacgggaa ctgcctgggc ggggggctcg
     4201 agctcgcgct ggtggccgac taccgcatgg catcggactc ccccaagacc atcctcgggt
     4261 tgcccgaggt ccagctcggc ctcatcccgg cggccggggg gacgcagagg ctcgtgcgac
     4321 tcatcgggct gagcgaggcc cttcccctga tgcttacggg caggaacctc aggcccaaga
     4381 aggcgctcaa gctcgggctc gtcgacgagg tggtctcggc gtacgggctc aaggaggcgg
     4441 gcctggcgaa ggcgagacag ctcgcatcgg gagcgaaatc gcccatgcgg gccaagaaga
     4501 ggctcaagga cagggtcctc gatgccacgg tcaagcgctt catagacgag acgaacgcgg
     4561 gcaggcggat cgtgttcaag aaggccaggg aacaggtgct caggcagacc atggggctgt
     4621 accccgcccc gctggccatc atcgattccg tggaatacgg gctcgccaac ggcatcgaca
     4681 aggggctcaa gagagacatc gaggtcttcg ccgatctcgt gatggacgac aagtcgaagg
     4741 ccttcaggag cctgttcgaa ggcatgaccg cgctcaagaa gaaccccctc aaggaccagg
     4801 cgcaaaaagt gcggaaactg gccgtcttgg gcgccggcct catgggccac ggcatcgtgt
     4861 ccgtctccac gggcatctgc gataccgttc tgctcaagga cgtctccctg gacgccgcgg
     4921 ccaagggcat gagggaggtc taccgcggcc tggacaagcg agcgcggtcg ggtgccatct
     4981 ccaggttcga gcgcgacctc cactacggga aggtggtgcc cacggacgac tactcgctct
     5041 ttcggaacgt ggacctggtg atcgaggccg tgttcgagga cctcgccctc aagcggaagg
     5101 tgctggccga ggtggaggcc gccgcctccg agaggaccat cttcgcgtcg aacacgtccg
     5161 cgctgcccat cacgctcatc gcagagggct gctcgaggcc ggccaacgtc atcggcatgc
     5221 actacttctc gccggttccc aagatgcccc tcctcgagat cataacgaca gagcacaccg
     5281 ccgactgggt caaggcgacc gcgctcgagt tcggcattca gcagggcaag acgtgcatcg
     5341 tcgtcaagga ctgccccggc ttctacacga ccaggatcct catccccatg ctcaacgaga
     5401 gcatgaggct cctcgaggag ggggcggaga tcagggcggt ggacacggcc atgcgcaggt
     5461 tcgggttccc tgtcggcccg gtcacgctcg tggacgagct ggggatcgac gtcgccgctc
     5521 acgttgccaa gggagaggtc aagaagctct tcgaggcaag ggggatcgag gcctccaacg
     5581 ggtacacgaa gctcttcgag aaggggttca aggggaggaa gaacaggaag ggcttctacg
     5641 tgtacgacga gcagaagaag ggcctgctcc cgctcgggag gaagaagggg ccgaaacagg
     5701 tcaataccga ggtgtacgag atcctgggcg ggcagaggaa ggccttcgat gagagcgaga
     5761 tccaggagag gctggccctg gcgatgatca acgaggcggc cctctgcctc gaggagggcg
     5821 tgctctcctg cgcccgcgac ggggacatcg gggcggtgtt cggcctcggc ttcccgccgt
     5881 ttacgggcgg gccgttcagg tacatcgaca ccgtgggggc gtcgtcggtg gtggacaggt
     5941 tgaagcggct cgaggacagg ttcggggccc tctacacgcc ggcgcggatc ctgctcgagg
     6001 cggccggatc gggcaagaag ttccgcgggt agtttggtgc aggcgatgcg gggtgcaggg
     6061 atgcaccccg cccttcctat gacatccttt ccggcttcaa cgagccggtg atcttttcgc
     6121 ctctttcggg gggcgtttcc gcttgtgcga ccctttcctc tccgagaaga acaatccacc
     6181 tcaacaggcg ggtgacgggg cgtgcccgcc tcgaggggcg aggggtgaac ccgtcgtcag
     6241 gtccgccctt gccgggggag tcggaaaggg cgaagggctg aagggatgtg ccgaggtcat
     6301 cgcctgccgt gcgcccggcg gggaaaaacg gccgccgtgc tgaggaacgg cggccgtgga
     6361 aaaaggaggg tgacaaaatg ttcgtcagga ggcctgcatg gcctttttca acgatctctt
     6421 caggtaatcg atgtgttcct ccacagccct gcacgcccgg gacgcgtctt tggatgcgat
     6481 cccctcgacg atctcgtgga gctgggacac gatgacccgt gagcttccct gtatggtgga
     6541 gagcttcttc ctgctgtagg ggaaggcccc cacgaggatg gtgttgatgg atctccggat
     6601 gtgcgcgaac gtggcgtttc tggtcgacgc gaggatggtg tcgaagaatt cggtgtagag
     6661 cctgcccccc tcctcgatgt cgtcgagcac ccgctccggc ccgatggcga gcgccctgtc
     6721 gaggatcttc ctgagcctct gcacgtccct ggccgtcgcg tgcgcgcacg cgagccggac
     6781 cgcctccgag tcgaggatcc tcctgacgca caagagctcc cagatcttct gctggtcggc
     6841 ggcgaggaga tcctcgatgg tgctcttcgg ggcgtcttgc gggatgcgga tgaactttcc
     6901 cctcctgcgc ctggactcga tgtaccccct ggcctcgagg agcttcatcg cctccctcag
     6961 cgagatgcgg cttatgcgga aacgcatggt catctcgtgc tccgagggga gacggtctcc
     7021 cggcttgagc ctccccttgc ggatgagctc gaggatctgc tccgcgacga gcgtgggtat
     7081 ggccggcgcc ttcttgatgg gcgatatgaa cctcttgacg gccctctttc cccgggatcc
     7141 cttcatagtc ttattgacat actaaaatac aaagcgggga aagaatcaac aggaaaaggg
     7201 gcggactttg ggggcgtgaa gggccgggtt caacgctcgg atcgcgccgc tgcacgagag
     7261 ggccgacggg ccgaggaata tcgcaggctc ggccttgacg gatcgccccg ccgcaggagc
     7321 gggccgacgt ctcggccgcc cacgggcccg cgcccccctt ctcgaacctc cccccggcat
     7381 gtcctttgcc acgggcatgc acgggcgtgg gctcactttc gatggcgatg gtgacgatgg
     7441 cggttgccac ggctcgacaa cgcgaaaggg ggatgtggcg tggagaccgg cgcccggctg
     7501 ccccttttct ctccccgggc ctgcgtgaac gagaggtggg cttgcgtccg ttctaggacg
     7561 gtcccggccg tggcggcacg ggaaaagagg ggcagggcgg tctcgtgcgg tatcggggtc
     7621 ggcctggggg tgagggcgtc tcaagggggc cgttccgccc tgtctcctcg tgggggggcg
     7681 tgaggcccct cgccgctgca tcagtccttt ttcttgccct tttcgagcgc ggtcaagagc
     7741 ttgtcgccga gggtcccgag cctgccctgg ggctgtgtgg agaaaggcct ccagtcaccc
     7801 tcctcgcagg tgtcggcggg tgagagggag atcctccttt tctccatgtc caccctctct
     7861 atggtcacgg tgaccgggtc gccctcgcgg agtttgtcca cgctcgatgc gagggcagag
     7921 cggtcgatca cggacctggg aaggagaccc gtgaccccgg gctcgatcct cacgaacacg
     7981 ccgaaccgct ccttcttctc gaccacgccc tcgaccacgc gccccaccgg gtatctccgg
     8041 tcgagctcga tccacgggtc cccctcggcc tccttcatgg acaaggagat ccttcgctgt
     8101 tcgggctgta tttccttgat catcacgtcc acctcgtcac ctgccttcac gaggtccgac
     8161 ggtctcttca cccgcgtggt gtaactcatc tcggacacgt ggacgagccc ctcgacgccg
     8221 ggcgctatct cgacgaacac gccgaagtcc gccgtccttg tgaccctgcc gcgaagtttc
     8281 tgcatgggcc tgaacctctc ggtgaccgtg ttccacgggt cctcgcccac ctgtttcatg
     8341 gacagcgaga tcttcctgcg gccggtgttc gggtccgctt ccacggccag gaccttcacc
     8401 gccaccctct cgcccacggc cacgatgtcc tcgggcgccg tctgcgcgcc gagcttgagc
     8461 ttgcggctgc ggcccgaggt caggaagacc tggcgcaccg gcacctgggt cgtcagaccc
     8521 agcctgttcg ctgcagccgc gccgtggccg acgacggttt cgccgcgctg gctggcccat
     8581 tcctggacca ccttggcgac ttccggtgcc cgtgcgccga atttgccctt cac
//
LOCUS       DAOSCR010000027         8488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5447_length_8488_cov_3.097000, whole genome shotgun sequence.
ACCESSION   DAOSCR010000027 DAOSCR010000000
VERSION     DAOSCR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_8488_cov_3.097000"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..727
                     /locus_tag="PLT69_01435"
     CDS             68..727
                     /locus_tag="PLT69_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13145.1"
                     /translation="MGSTRGKRRSSGGTLARIVRAATVEFAEKGFDGARVEAIAKRAG
                     VNKAALYYHVGNKKALYERVILNVVGAAAVRLDEKLSGTSRADEMLRTYVRVLMQTFD
                     EHPEMPRIIMREMASGGSHLPEAFLKGVSGILDRLAKILDAGRKEGVFEEVMPLVVHL
                     MVVGAAAISRHMPARLKSMLRAASHLGGTGGERLMGEVAGEIERLVIKAVSASKARPG
                     G"
     gene            730..1740
                     /locus_tag="PLT69_01440"
     CDS             730..1740
                     /locus_tag="PLT69_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ13146.1"
                     /translation="MRVRKVVVILALLLAAVGVIVWVGQRGSADGDLVYSGTIEATDI
                     GLAFQTSGRVREVLAQEGMRVDKGAVLARLDDAELASRVLMAKANLERAERLAEQLAT
                     SLEVMKSVLPQDLRRAWAVLDRARSAEREARRNKERYERLYAEGVVSEKERDAYRLGH
                     DTALSAVREAEAARIQAEAALEKIEATKKELEAAKAQVLSSRSSLEQATIQLGYATLV
                     SPVTGVVTSRNVEPGEVVNPSREAITVTDLSRVDLKIFVEETAIAKVRPGARVEVTVD
                     SFPGKVYHGVVSYVSPEAEFTPKFIQTRKERVKLVYLVKVSLENPNYELKTGMPADAR
                     IR"
     gene            1724..2473
                     /locus_tag="PLT69_01445"
     CDS             1724..2473
                     /locus_tag="PLT69_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13147.1"
                     /translation="MHGSGEGVVVVESVSMRYGTVKALDGVSLVVRRGTIFGIIGSDG
                     AGKSTLLRLVAGMEKPHEGRVLVGGVDTATGRIRREGLVGYMPQRFGLYQDLSVAENM
                     DFFLDVIGVRGQDRKRRKSTYLGFSGLEPFSDRLAGNLSGGMKQKLALACVLLTEPSC
                     LVLDEPTNGVDPVSRREFWDILVHMRERGMAVVVATSYPEEGDLCDEVAFMHRSRIVK
                     AGDPASFKGSHPSIEAAMVALLETMEEGSSP"
     gene            2470..3225
                     /locus_tag="PLT69_01450"
     CDS             2470..3225
                     /locus_tag="PLT69_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13148.1"
                     /translation="MTGGSEDVIVVDRLEKRFGDFTAVKGISFTVRRGEIFGFLGSNG
                     SGKSTTIRMLCGIISPTSGSASVAGIDVVRRPEEVKRVIGYMSQKFSLYEDLTPVENI
                     RFYLGLYGVDPKRWDERIGWVIENAYLADMKDRLTRDLPQGFRQRLALGCALVHEPGI
                     LFLDEPTSGVDPLTRRRFWDSIRALADQGVTVFVTTHYMDEAQNCSRIVMIDLGAVVA
                     QGSPSEIVSGACPGRPGARLEDAFVALMGGGGR"
     gene            3228..4355
                     /locus_tag="PLT69_01455"
     CDS             3228..4355
                     /locus_tag="PLT69_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ13149.1"
                     /translation="MAVVRAVARKEFLHLVRDIRSLVLAFLFPLLLIMLFGYALSLDV
                     NDVPLVVVDHDHTPASRDLVRSLDASLYFRVIDRLERTSDAARYLDRAAAVMAVIVPP
                     GFASRIASGRSSPLQVIIDGSDPNVASIARGYLSSFVEGRNSRLLLDYLSRNNMEPVV
                     APVEPRVRVWFNEDLESTRFIVPGIIAIIIMIAGAMLTSLVIAREFENGTMETVKSLP
                     ISAGEFLAGKAVFYFMIALVDVLVAILLGQVLFGAVMKGGFLFMIAASSLYIMVALSL
                     GLFISAAVKSQLLANQAAILLTYLPSFLLSDFVFPISNLPAPLRALTYIVPARYFMSV
                     LAGIYLRGTGPVELWRDIAVLCAMFVVLTLLCVKILKKEGM"
     gene            4352..5473
                     /locus_tag="PLT69_01460"
     CDS             4352..5473
                     /locus_tag="PLT69_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ13150.1"
                     /translation="MSLVRVRELVIKEFIVLFRDRMNRRILFMAPIIQIVIFGYVVNY
                     DVKDVTLAVFDQANTSQSRELVRAFDASPVFRVTLRPGNDRELEEALLEKKVDLALKI
                     PPDFSVRIRSGKSAPVQLIADGTQSNMAQIRLAYSQAVVSGYNVRALQRIAGTKLAYG
                     RIDDRIRTWYNPNLDSSLFFVPGIVAFIVMLISLLLTSIAVIREKEQGTMEQLLVTPL
                     TPAEMILGKTIPYIVISIAQMALITAFARLWFDIPMAGSTLLLLAATCLFLLSTLGIG
                     LFVSTVSATQQQAMMTTFFVMLPAFMLSGFIFPIANMPLAVRIVTYLNPLRYFLEVVR
                     GIFLKGVSLDVLWPQYAAMAVLGAVFLSGAVARFHKRLD"
     gene            complement(5872..6420)
                     /locus_tag="PLT69_01465"
     CDS             complement(5872..6420)
                     /locus_tag="PLT69_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOJ13151.1"
                     /translation="MKTLVLGMGNPILGDDGIGIAVARALGDLLPGVDVAVCTTIGLD
                     LMDLFSGYDSVVVVDAMCTRTGKPGEVRQIDPDSGPGTLHLFSSHGLHFFQVMDLGRR
                     LGLKMPTSTRVFGIEIGDMVSFDEALSEGLRGDLGRIVQAIAKEVAGLPCTSTNGTPT
                     KGEDDEPAPTDGQAISRENRRP"
     gene            complement(6500..7960)
                     /locus_tag="PLT69_01470"
     CDS             complement(6500..7960)
                     /locus_tag="PLT69_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOJ13152.1"
                     /translation="MTQRITIDPITRLEGHGKIEIFLDEKGEVKDAYWQVVELRGFER
                     FCLGRPAEEMPRIVPNICGVCPSAHHMAATKALDDLYSVEPTPAARLIRELEFNAFYI
                     EDHYIHFFFLAAPDFIVGPEADPRERNVLGVIARVGKEIGSKVIEVRRKNRDIIRHIF
                     GKAPHPEGGLPGGVPRGFTEEDRKWVVSTAESSLEFAKFAIQLFKDAVLSDKSCVDLI
                     MNDAYSLETYYMALVDDLNRTTFYDGTVRVVDKDGKEYTKFHPRDYDKVIGEWVEPWT
                     YIKLNHLKTLGWHGLKDGDGTSLYRVAPLARLNVGDSMLTPLAHKESQIMFDTLGGKP
                     AHQTLATHWARLICALQAAELNVEIANHPLLTSKDIRNMNLRLRHKGVGCVEAPRGTL
                     FHHYETDDDGILTKVNLIVATQNNAAPICLSIKKAAKAFVKGPDVREGLLNMVEMAFR
                     AYDPCLSCATHSLPGSMPLTVNIRDHEGTLLRTIKR"
     gene            complement(8027..>8488)
                     /locus_tag="PLT69_01475"
     CDS             complement(8027..>8488)
                     /locus_tag="PLT69_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOJ13153.1"
                     /translation="PPPPDLVANAVSAVLEGKLPPRGSTLAPHRPLCDTCPRNESKPP
                     KIEIPEVRRVHMVEADEGKCFLAQGLLCLGPATRSGCGERCIRVNTPCRGCFGPVEGV
                     DDGGAKFVSCLAALLSAEDEAEATRVTDGIADLPGTCYRFTAASISLKGKS"
BASE COUNT         1445 a         2635 c         2824 g         1584 t
ORIGIN      
        1 cctggtgagg aaaaggggat gttgacaggg gcctccttgt catataacta aacggttggt
       61 taaaaacatg ggatcgaccc gaggaaagag gcggagttcc ggcggcaccc tcgccaggat
      121 cgtgcgcgcg gccacggtcg agttcgcgga aaaggggttc gatggcgccc gggtggaggc
      181 gatagcgaag agggcggggg tgaacaaggc cgcgctgtac taccacgtgg ggaacaagaa
      241 ggcgctctac gagcgggtca tcctgaacgt ggtcggcgcg gcagcggtca ggctcgacga
      301 gaaactctcg gggacgtccc gcgcggacga gatgttgcgc acgtacgtga gggttctcat
      361 gcagaccttc gacgaacacc cggagatgcc acggatcatc atgcgcgaga tggcctcggg
      421 ggggtcgcac ctgcccgagg ccttcctgaa gggggtctca ggcatcctgg acaggctggc
      481 gaagatcctc gacgccggca ggaaggaagg ggtgttcgag gaggtcatgc cgctcgttgt
      541 gcacctcatg gtagtcggtg cggccgcgat ctcacggcac atgccggcca ggctgaaatc
      601 catgctcagg gctgcatccc acctgggcgg cacgggtggg gaaaggctca tgggagaggt
      661 tgccggggag atcgagcgcc tcgtcatcaa ggccgtctcg gcctccaagg ctcgaccggg
      721 agggtgagca tgcgtgtgcg caaggttgtc gtgatcctgg ccctgctgct ggccgcggta
      781 ggcgtcatcg tgtgggtcgg tcagagaggc tcggcagatg gcgacctggt ctactcgggc
      841 accatcgagg ccacggacat agggctcgcc ttccagacct cgggacgggt gagggaggtc
      901 ctggctcagg agggtatgcg cgtggacaaa ggcgcggtgc tcgcccggct cgacgacgcg
      961 gaactcgctt ccagggtcct catggcgaaa gcgaacctcg agcgcgcgga gaggctcgca
     1021 gagcagctcg ccacctcgct cgaggtcatg aagtccgtgc ttccgcagga cctcaggagg
     1081 gcttgggccg tcctggacag ggcaaggagc gcggagaggg aggcgaggag gaacaaggag
     1141 cgttacgaaa ggctctacgc cgagggggtg gtctccgaga aggagaggga cgcctacagg
     1201 ctcggccacg acaccgccct ctcggcggtg agggaggccg aggccgcccg catccaggcc
     1261 gaagccgcgc tcgagaagat agaggccacg aagaaggagc tcgaggcggc caaggcccag
     1321 gtgctctcgt cccgttcctc gctggagcag gccaccatac agctcggcta cgccacgttg
     1381 gtctccccgg tcacgggggt ggtcacgagc aggaacgtgg agcccgggga ggtggtcaac
     1441 ccctccaggg aggcgatcac cgtgaccgac ctgtcgaggg tggacctgaa gatcttcgtg
     1501 gaggagaccg ccatagccaa ggtgagaccc ggggcgcgcg tcgaggtcac ggtggactcg
     1561 tttccgggca aggtctacca cggggtggtg agctatgtct cgcccgaggc cgagttcact
     1621 ccgaagttca tccagaccag gaaagagcgc gtgaagctgg tctacctcgt gaaggtctct
     1681 ctcgagaacc cgaactacga gctcaagacg gggatgcctg cggatgcacg gatcaggtga
     1741 aggggtcgtc gtcgtagaat ccgtgtccat gcgctacggc acggtcaagg ccttggacgg
     1801 cgtgagcctc gtggtgagga gggggacgat cttcggcatc atcggttccg acggcgcggg
     1861 gaagtcgact ctgctcaggc tcgtggccgg catggaaaag ccgcacgagg gccgcgtcct
     1921 ggtggggggg gtcgacaccg ccacgggccg tatccgcagg gagggcctcg tggggtacat
     1981 gccccagagg ttcggcctct accaggacct gagcgtggcg gagaacatgg acttcttcct
     2041 cgacgtgatc ggcgtgaggg ggcaggacag aaagcgcagg aagagcacgt acctggggtt
     2101 ctcggggctc gagccctttt cggaccgcct cgcggggaat ctctccggcg gcatgaagca
     2161 gaagctggct ctggcctgcg tgttgctcac ggagccctcg tgcctggtgc tggacgagcc
     2221 caccaacggg gtggaccccg tctccaggcg cgaattctgg gacatcctcg tgcacatgag
     2281 agagaggggc atggcggtcg tcgtggcgac ctcttacccc gaggagggcg acctgtgcga
     2341 cgaggtggcg ttcatgcacc gttcgaggat cgtcaaggca ggcgacccgg cgtcgttcaa
     2401 gggatcgcac ccgagcatcg aggcggccat ggtggcgttg ctcgaaacca tggaggaggg
     2461 ctcgtccccg tgacaggcgg cagcgaagac gtgatcgtcg tcgacaggct cgagaagcgc
     2521 ttcggggact tcacggcggt caaggggatc tcgttcaccg tgaggagggg ggagatcttc
     2581 ggcttcctgg ggagcaacgg cagcggcaag tcgaccacca tccgcatgct ctgcggtatc
     2641 atctccccca cctcaggcag cgcctcggtg gcgggcatcg acgtggtgag acgccccgag
     2701 gaggtcaagc gggtcatcgg gtacatgtcc cagaagttct ccctctacga ggacctcacg
     2761 ccggtggaga acatcaggtt ctacctgggg ctgtacggcg tcgacccgaa gaggtgggac
     2821 gagcggatcg ggtgggtcat cgagaacgcg taccttgcgg acatgaaaga ccgcctcacg
     2881 cgcgacctgc cccaggggtt caggcagcgc ctggcgctcg ggtgcgccct cgtccacgaa
     2941 ccgggcatcc tcttcctcga cgagcccacc tcgggggtcg acccgctcac caggaggagg
     3001 ttctgggact ccatccgggc gctggcggac cagggggtga cggtgttcgt gaccacccac
     3061 tacatggacg aggcacagaa ctgctcgcgc atcgtcatga tcgacctggg tgccgtcgtg
     3121 gcccagggga gcccgtccga gatcgtctcc ggcgcgtgcc cggggaggcc aggtgcgagg
     3181 ctggaggacg ccttcgtcgc cctcatggga ggtggcgggc ggtgaagatg gctgtcgtga
     3241 gggccgtggc ccgcaaggag ttcctgcacc tggtgaggga catcaggagc ctcgtgctcg
     3301 ccttcctctt ccccctgctc ctcatcatgc tcttcggtta cgcgctgagc ctggacgtga
     3361 acgacgtccc cctcgtggtc gtcgatcatg accacacccc tgcgtccagg gacctcgtga
     3421 ggtccctgga cgcgtctttg tatttcaggg tcatagaccg tctcgagagg acctcggacg
     3481 cggcgcggta cctggacagg gctgcggcgg tcatggcggt gatcgtgccg cccgggttcg
     3541 cctcgaggat cgcatccggc agatcgtccc cgctccaggt catcatcgac gggagcgacc
     3601 cgaacgtcgc gagcatcgcg aggggctacc tgtcgtcctt cgtggagggc aggaactcgc
     3661 ggctgctcct ggactacctg tcgcgcaaca acatggagcc ggtcgtggcg ccggtcgagc
     3721 ccagggtcag ggtctggttc aacgaggacc tcgagagcac caggttcatc gtgcccggga
     3781 tcatcgccat catcatcatg atcgccggcg ccatgctcac ctccctcgtg atagcgcgcg
     3841 agttcgagaa cggcaccatg gagacggtga agtccctccc cataagcgcg ggcgagttcc
     3901 tggccggcaa ggccgtcttc tacttcatga tcgcgctcgt ggacgtgttg gtggcgatcc
     3961 tgctcgggca ggtcctcttc ggcgcggtca tgaagggggg cttcctcttc atgatcgccg
     4021 cctcgtcgct ctacatcatg gtggccctct cgctggggct cttcatctcc gccgccgtca
     4081 aatcgcagct cctggcgaac caggcggcga tcctcctcac gtacctcccg tcgttcctcc
     4141 tgtccgattt cgtgttcccg atcagcaacc ttcccgcccc gctcagggcg ctgacctaca
     4201 tcgtgcctgc gcgttacttc atgtcggtgc ttgccggcat ctacctcagg ggtaccggcc
     4261 cggtcgagct gtggagagac atcgcggttt tgtgcgccat gttcgtcgtt ctcacgctcc
     4321 tgtgcgtgaa gatcctcaag aaggagggca tgtgagcctc gtcagggtca gggagctggt
     4381 catcaaggag ttcatcgtcc tgttcaggga caggatgaac aggaggatct tgttcatggc
     4441 gcccatcatc cagatcgtga tcttcgggta cgtggtcaac tacgacgtga aggatgtgac
     4501 gctcgccgtc ttcgaccagg cgaatacgag ccagagcagg gaactcgtcc gcgctttcga
     4561 cgcgagcccc gtgttcaggg tgactctcag gcctggaaac gacagggagc tcgaggaggc
     4621 cctgctcgag aagaaggtgg acctggccct caagataccg cccgatttct ccgttcgcat
     4681 ccgctccgga aagagcgccc ccgtccagct catcgcagac ggcacccaga gcaacatggc
     4741 ccagatcagg ctcgcataca gccaggcggt ggtgagcggg tacaacgtga gggcgctcca
     4801 gaggatcgcc gggacgaagc tcgcctatgg gaggatcgac gaccgcatac ggacgtggta
     4861 caacccgaac ctcgacagca gcctgttctt cgtgccgggc atcgtggcct tcatcgtgat
     4921 gctcatctcc ctgctgctca cgtccatagc ggtgatcagg gagaaggagc agggcaccat
     4981 ggaacaactc ctcgtcaccc ccctgacgcc cgcagagatg atcctcggca agaccatccc
     5041 gtacatcgtg atctcgatag cccagatggc cctgatcacc gcattcgcga ggctgtggtt
     5101 cgacatcccc atggccggaa gcaccctgct cctgctcgcg gccacgtgcc tgttcctttt
     5161 gagcacgctc gggatagggc tcttcgtctc gacggtctcg gccacgcagc agcaggccat
     5221 gatgacgacg ttcttcgtca tgctgccggc gttcatgctg tcgggcttca tcttccccat
     5281 agccaacatg ccgttggccg tcaggatcgt gacctacctg aacccgctga ggtacttcct
     5341 cgaggtcgtg cggggcatct ttctcaaggg cgtgtccctg gacgtgttgt ggccccagta
     5401 cgcggccatg gccgtactcg gcgcggtgtt cctctccggc gccgttgcca ggttccacaa
     5461 gaggctcgac tgatcccgcc ttgtgccgcg ctgcccggca cgatggagac ccggggtgga
     5521 aggaatccgg ggtcgtacct cgcccatcag ggccccggac aggtgcatgg gcgatccctc
     5581 cgtggtgttc cgcgtcatcg ggtccgggcg taaagacgac accctcaccg gagagctacc
     5641 aggcggctca ttgacggtca cgaggttgcg gctgaggcct cgaacccatg ccttcccacc
     5701 gtttgtgggg ggcttgccgc tctcccgggc catgcctgga gagcctcgtt ccggcgctct
     5761 ttcctcaccg tgaagagggc cttggagaac aggactcgct gagaagaggg acttggagaa
     5821 gaggattctt tgagaggtgc ttcttcttct gcctcgattc tcctgcgccg ctcagggtct
     5881 ccgattctcc ctactgatag cctgcccatc cgtcggggca ggctcgtcat cctccccctt
     5941 ggtcggggta ccgtttgtcg atgtgcaagg cagacccgcc acctccttcg caatggcctg
     6001 cacgatacgg ccgaggtctc cccgaagccc ctcggacagc gcctcgtcga aggataccat
     6061 gtcgcctatc tcgatgccga agaccctcgt cgaggtgggc atctttagcc caagcctgcg
     6121 gcccaggtcc atgacctgga agaagtgcaa gccgtgagaa ctgaagaggt gcagggtgcc
     6181 cgggccggag tccggatcga tctgcctcac ctcgcccggc tttccggtcc gtgtgcacat
     6241 ggcgtcgacc acgacgacac tgtcgtatcc tgagaagagg tccatgagat cgaggcctat
     6301 ggtcgtgcac actgccacgt cgaccccggg cagcaggtct cccagcgctc gcgcgacggc
     6361 tatccctatg ccgtcatccc cgagtatcgg gttgcccatc ccgagcacca gggtcttcac
     6421 ggccctcacc ccccagaccg gtgcgcaagg ttaaacggcg accatccatc catcccgggc
     6481 cccatggggt tcatgggggt cacctcttta tggtcctcag cagggtcccc tcgtgatccc
     6541 tgatgttcac cgtcaggggc atggagccgg gaagggagtg cgttgcgcag gacaggcacg
     6601 ggtcgtacgc cctgaacgcc atttcgacca tgttgagcag gccttccctc acgtcggggc
     6661 ccttgacgaa ggccttcgcc gccttcttga tcgagagaca gatgggcgcc gcgttgttct
     6721 gcgtggcgac gatgaggttc accttggtga ggatgccgtc gtcgtccgtc tcgtagtggt
     6781 ggaagagggt tcccctgggg gcctccacgc accccacgcc cttgtgcctc agcctcaggt
     6841 tcatgttgcg gatgtccttg ctcgtgagaa ggggatggtt cgcgatctcc acgttgagct
     6901 cggccgcttg cagcgcgcag atgagcctcg cccagtgggt ggcgagggtc tggtgggcgg
     6961 gtttccctcc cagggtgtcg aacatgatct gcgactcctt gtgggcgagc ggggtgagca
     7021 tggaatcgcc gacgttgagc cgggcgagag gcgcaacgcg gtacagcgag gtgccatcgc
     7081 cgtccttgag accgtgccag ccgagggtct tcaggtggtt gagctttatg taggtccacg
     7141 gttccaccca ctcccctatc accttgtcgt agtcgcgggg gtggaacttc gtgtattcct
     7201 tcccgtcctt gtcgacgacc ctgacggtgc cgtcgtagaa ggtggtccgg ttcaggtcgt
     7261 caaccagggc catgtagtaa gtctcgaggc tgtaggcgtc gttcatgatg aggtcgacgc
     7321 acgacttgtc cgacaggacg gcgtccttga agagctggat cgcgaacttc gcgaactcca
     7381 gcgacgactc ggccgtgctg accacccact tcctgtcttc ctcggtgaac cccctgggta
     7441 cgccgcccgg caggccgccc tccgggtgag gcgccttccc gaagatgtgc cgtatgatat
     7501 cccggttctt cctcctcacc tcgatgacct tgctgccgat ctccttgccc accctggcga
     7561 tcacccccag gacgtttcgt tccctcgggt ccgcctccgg ccccacgatg aagtcgggcg
     7621 cggcgaggaa gaagaagtgg atgtaatggt cctcgatgta gaatgcgttg aactcgagct
     7681 ccctgatcaa ccgtgcggca ggggtcggtt cgacggagta gaggtcgtcg agggccttgg
     7741 tcgcggccat gtggtgtgcc gacgggcata cgccacatat gttcggcacg atcctcggca
     7801 tttcctcggc cggcctgccg aggcagaagc gttcgaaacc tctcagttcc acgacttgcc
     7861 agtaggcgtc cttaacctcg cccttctcgt cgaggaagat ctcgatcttc ccatgccctt
     7921 cgagcctggt tatggggtct atggtgatcc tctgcgtcat ctcttcgtcc ttcgtatgtg
     7981 gatatggcgt ttccatccaa gcttgcggcc cacgggaaga cccaggtcac gatttcccct
     8041 tgagggatat cgatgcggcc gtgaacctgt agcatgtacc cggcaggtcg gctatgccgt
     8101 ccgtgaccct cgtcgcctct gcctcgtcct cggccgaaag aagcgctgcg aggcaggaga
     8161 cgaacttggc gcccccgtcg tcgaccccct cgacagggcc gaagcacccc ctgcacgggg
     8221 tgttgaccct gatgcacctc tcgccgcagc ccgatcgcgt ggcaggaccc aggcacagga
     8281 gaccctgggc caggaaacac ttgccctcgt ccgcctcgac catgtggacc cggcggacct
     8341 cggggatctc gatcttgggc ggttttgact cgttccgggg acaggtgtcg cacaggggcc
     8401 tgtgcggcgc gagcgtggag ccgcggggcg gcagcttgcc ttcgagcacc gcggagaccg
     8461 cgttcgccac gaggtccggg ggaggggg
//
LOCUS       DAOSCR010000028         8432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5498_length_8432_cov_3.856512, whole genome shotgun sequence.
ACCESSION   DAOSCR010000028 DAOSCR010000000
VERSION     DAOSCR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5498_length_8432_cov_3.856512"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..369)
                     /locus_tag="PLT69_01480"
     CDS             complement(52..369)
                     /locus_tag="PLT69_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOJ13154.1"
                     /translation="MEYDLKDLFRQAARIQEKIKEIQEQLSRRTVTVDTGGGMVTVTV
                     NGRQEVVSIKLDPQCVDPRDIPMLEDLILTAVNLGMKRSRELAAEEMKHLTGPYPLPF
                     TVT"
     gene            991..1776
                     /locus_tag="PLT69_01485"
     CDS             991..1776
                     /locus_tag="PLT69_01485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOJ13155.1"
                     /translation="MKRTFIDLSIAIESGLPSDPPMMIPKITYLDHALGAETMQLFFP
                     GVTKDDLPGGLGWALEILELSTHAGTHMDAPWHYHPTQDNGRPALTIDEFPLEWAVGE
                     GVKLDFSDLPDGYNVTPDDIRRRLDAIGHTLAEGDIFLAQSGAAPFWGTPEYLVKGCG
                     FGREATLWLVEKGVHVVGTDAWSWDRPLAFQAEEFSRSRDPSVIWEGHFAGRERGYFQ
                     IEKLTNLHLLPSTGFTFYCFPVKIKGASAGWIRAVAEIHTQGR"
     gene            1848..2645
                     /locus_tag="PLT69_01490"
     CDS             1848..2645
                     /locus_tag="PLT69_01490"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOJ13156.1"
                     /translation="MLAIDVGNTAITAGIFEDGFLRGTVRVSTRECIAKGSFLGLTEG
                     IPREAAKDAVVVGVRAEALEIVRADLGATGADPLVVDVHTRMGIENAYASVQTLGPDR
                     LVAASAAFHLHGGKGAPLVVVDMGTATTIDYVSEQGVFMGGVIAPGMMSAYRGLLAEA
                     PGLPRIEEFDDPPLIGRDTAGCLKAGVVTAHAAMIVKTAFLMGEETGKIPHVVVTGGL
                     SVFVKKKVPSSFIFDEHLILRGLFLVHSLTKGQGCKVEGPREPIHDR"
     gene            2675..4465
                     /locus_tag="PLT69_01495"
     CDS             2675..4465
                     /locus_tag="PLT69_01495"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ13157.1"
                     /translation="MDDTFGSVMDYTARRLLCCLEVLLLIVLVGLPPQAFASTSAAEA
                     YQSANSAAVELYRDASKRKDRVSWMAVIHKFQNVAKRHPDSGYAAKALFKTGKLYEDL
                     HRFSSRPDDLEKAADTYLALAEKYPKNQLADDAVYSAARIHHLSLGSPVKASSLYQRV
                     IRDYPEGDMVQLAREQMNALNVEKRTVTAPSLGGPGKVRSLNQWSDRDYTRVVVELDD
                     NVTFKTFTLPADASANRPERVILELDNARISPSIPALTRVDDGILKNIRASQFSADKV
                     RIVLDLARKVTYRAFPLSDPSRMVVDIVKKGAPMSVAAGDSRQKTPAEPLGMSTGGVQ
                     RPEAKNGFKKVPKWSQSQMSDDVPSIAAQLSLKVSRIVVDPGHGGKDPGAIGPGGVKE
                     KDLTLAIGKALARRLRHEGFEVFLTRETDVFIPLEERTAFANKKRADLFVSIHINAHN
                     NTSLQGIETYFLNLTTDASAIEVAARENATTSKSISDLQLIINDLMLNSKINESSRFA
                     TSVQKCIMSSLINDGYQGKDLGVKQAPFYVLLGTQMPSILCELGFLTNCTEADLLQNE
                     AHQNALVDGIAKGINKYIMNTTYAYLRSAK"
     gene            4462..5202
                     /locus_tag="PLT69_01500"
     CDS             4462..5202
                     /locus_tag="PLT69_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOJ13158.1"
                     /translation="MRGFILTAAVFVLCAVCPAAWAGYGLSNVVEKTIAQPDSSQAPP
                     TSKPQAQEAPPAQPGAKQAPQEAKPASTATAPEPLPTPAKKPAITYEKTAFYPYTIHI
                     SSWQSKKDALNHMQRLSGELEGVFVTKIDLGPTGLWYRVDYGVFENDQQAGQTMAELK
                     TKGVLRNDPFIGSPVPYAIEIGVFPSKDAASAEASRLIRKGVSTYVVKESEAAYRLLS
                     GAYHDRKSAEPALADLAALGIRAKTAKR"
     gene            5199..6083
                     /gene="xerD"
                     /locus_tag="PLT69_01505"
     CDS             5199..6083
                     /gene="xerD"
                     /locus_tag="PLT69_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOJ13159.1"
                     /translation="MNRSLVDSFLEYLVAERSSPENTLRAYALDVADYLSFLESRGIA
                     AADAEAWTLEDYAVHLRASGLKATSVTRKLSAIRHFYRFLLEEDAAERDPTKDVASPK
                     RVRTLPEVLSREEVERLVESPDCSTPQGVRDRAMLEVLYATGLRVSELVGLRLHDVDL
                     AVGYVLSRGKGDKERIVPLGESARRWLARYVGEARPRLVKKSTDVLFCSRLGKAMTRQ
                     NVWHAIKRHARHAGIAKRISPHTLRHSFATHLLVGGADLRSVQEMLGHADISTTQVYT
                     HVTSPRLKEIHRRYHPRG"
     gene            6087..>8432
                     /locus_tag="PLT69_01510"
     CDS             6087..>8432
                     /locus_tag="PLT69_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ13160.1"
                     /translation="MEVITSHIQADFDAFASMLAARILFPEALLVFPGAQEKNLREFV
                     SQLDPGLSGAIAKLKSIDLESVRRLVIVDTRQLSRIGDFARIVGRPHVEVITYDHHPP
                     SNDDIVTKDSHVREYGSNSTLMVEELKGRGLVPPSEVATILAMGIFEDTGSLLFPSTR
                     PEDCMALAQLLQWGADLTHVSRSLARDLSPDQVDVLDQLIKNAAIHHVGGMDVLVTTS
                     ESTGYIEDFAMLVHKLRGMFQVDAVFAAGLMGERIYLVARSTVPQINAAEIAARFGGG
                     GHPTAAAASISGRSLQSVAGTLVTIIKKETRPRITARDIMTFPVISLKMNATIDEAAQ
                     TLNRYNINAAVVQSRDHKAVGLVTRQVVTRAQGHGLGGAPVKDYMIRDIQSVGPEAPV
                     GEIRTLIIDGNQRLLPVIENKKAIGVITRTDLMRVMHEKMAKTEGIDLKASQRKNVAS
                     LMAERLPREIVEMLRDAGIQASSMGFNVYLVGGIVRDMVMRIDNLDIDLVVEGDGIAF
                     ARAFSSKVGIRCAVHEKYKTAVITLPDGRHIDVATARTEYYKHPGGYPVVEQATLKLD
                     LYRRDFTINTMAVALVPQRFGELIDFFGAQRDIKDRRIRVLHALSFVEDPSRILRAVR
                     FEKRYGFTLGKQTLALVHAAVRSGLLSSIPGRRISHEIRQILSEEDPVACLERLKELD
                     VLEAVHPDLTFTPEVREIMLSVKQTISWFELLYTHEPYRPWFIYLLGLVDTMKPKKIV
                     EICQRLGLTPENIRTILSSRSRVNAVLRDLATDGEKRPSNVA"
BASE COUNT         1654 a         2774 c         2679 g         1325 t
ORIGIN      
        1 cgcctgagag cggagatgag gtccttgagg ggcctggggt agggatccat gtcacgtcac
       61 ggtgaagggc agagggtatg ggcccgtgag gtgtttcatc tcctccgcgg cgagctcgcg
      121 ggacctcttc atccccaggt tcaccgcggt caggatgaga tcctcgagca tggggatgtc
      181 cctcgggtcg acgcattgag ggtccagctt gatggaaacg acctcctgcc tgccgttgac
      241 ggtcacggtg accatgccgc cccccgtgtc gacggtgacc gtcctgcgcg agagctgctc
      301 ctggatctcc ttgatctttt cctggatgcg ggcagcctgc ctgaagagat ccttcagatc
      361 gtactccatg gtcagcaagt aaaccgtaaa ggccgagggt gtgtcaagca ggcagcaatc
      421 ccgtgccccc aacgacgaca agcaggtgat cgtccccagg gcctcggact ccccggcaaa
      481 gacccgccgc cccgatctct tctttcgacc tgcgttcggg caacgggagg cgcccctcag
      541 ccagatcacg catcgagcag agatccacca ggacgacctc catgaacctc catcggggaa
      601 ggtcgccgac gggaacaggg gtgtcgcccc atctggttcg tgggacatcg tggacccttg
      661 ggcttgcgaa caaccaccga ggaagacggc cttcggtgcg gcaccctcaa ggttcacgac
      721 cgcgagtgct tccgtgcgtt cgagacggcg catcctgccg cacccccatg caggtcccga
      781 gacggccgcg gcctcggcgc tccgcgagga gcaccgggcg gacgctcacc tgcacccgca
      841 cccgcacatc cactctctgc gaggatcttg acactcggcc ggggccatga gaatcatatg
      901 cacgatgaga caccgccgcc tgcccacgtg gaccggcctt cttcggcagg cggcgggatg
      961 ccgacgcgca cgacccgggg ggaggaagcc gtgaagcgca ccttcatcga cctgtcgatc
     1021 gccatagagt ccgggctgcc cagcgatccg cccatgatga tcccgaagat cacctacctc
     1081 gaccacgcac tgggggccga gaccatgcag ctgttcttcc cgggcgtcac gaaggacgac
     1141 ctcccgggcg ggctcgggtg ggctctcgag atcctcgagc tctccacgca tgcgggcacg
     1201 cacatggatg cgccctggca ctaccacccc acgcaggaca acggcaggcc cgccctcacc
     1261 atagacgagt tccccctcga gtgggccgtc ggcgaggggg tgaaactcga cttctccgac
     1321 ctgcccgacg gctacaacgt gacacctgac gacatccggc gcaggctgga tgcgatcggg
     1381 cacaccctcg ccgaggggga tatcttcctc gcccagtccg gcgccgcacc cttctggggc
     1441 actccggaat acctcgtcaa ggggtgcggg ttcggcaggg aggcgaccct gtggctcgtg
     1501 gagaaggggg tgcacgtggt cggcacggac gcctggagct gggaccgccc gctcgcattc
     1561 caggccgagg agttctcgcg aagccgtgat ccctcggtca tctgggaagg acacttcgcc
     1621 ggcagagaac ggggctactt ccagatcgag aagctcacga accttcacct gctgccgtcc
     1681 acggggttca cgttctactg cttccccgtc aagatcaagg gtgcgagcgc gggctggata
     1741 cgtgcggtgg cggagataca cacccagggc cgataggccg agaccaggcg tccgggaggc
     1801 cctcgaaagc acttgatatg cccctcgttt aggtctacac acaagccatg ctcgccatcg
     1861 acgtggggaa cacggctatc acggcaggca tcttcgagga cggcttcctc agggggaccg
     1921 tcagggtatc caccagggag tgcatagcca agggatcttt cctgggactg accgagggga
     1981 tcccacgcga ggcggccaag gacgcggtgg tggtgggcgt acgggccgag gccctcgaga
     2041 tcgtccgcgc agacctgggg gcgacgggcg cagacccgct cgtggtggac gttcacaccc
     2101 gcatggggat cgagaatgca tacgcgagcg tgcagaccct cgggcccgac cgcctcgtgg
     2161 cggcctcggc cgcgttccac ctgcacggag gcaagggggc tcccctcgtg gtggtggaca
     2221 tgggcacggc caccaccatt gactacgtga gcgaacaggg cgtgttcatg ggcggcgtga
     2281 tcgcccccgg catgatgagc gcatacaggg ggctgctcgc cgaggcgccg gggctgccgc
     2341 gcatcgagga gttcgacgac ccccccctga tagggaggga cacggcggga tgcctcaagg
     2401 cgggcgtcgt caccgcgcac gccgccatga tcgtgaaaac ggcgttcttg atgggggaag
     2461 agaccggtaa aatcccgcat gtggtggtta ccggtggatt gtcggtattt gtaaagaaaa
     2521 aagtgccgtc atccttcatt ttcgacgaac acctcatctt gagaggactt ttcctggtcc
     2581 attccctcac caagggacag ggctgtaaag tcgaggggcc tcgagaaccg atacatgaca
     2641 ggtagggtgc ccaagccggc acaggaaacg agtaatggat gacacgttcg ggagcgtaat
     2701 ggattacacc gcacgaaggt tgctctgctg cctggaggta ctccttctca tcgtcctggt
     2761 tgggttgccg cctcaggcct tcgcctccac gagcgccgcc gaggcctacc agagcgccaa
     2821 ctcggccgcg gtcgagttgt accgcgacgc ttcgaagcgg aaggaccgcg tgagctggat
     2881 ggcggtgatc cacaagttcc agaacgtggc caagcgccac cccgactccg ggtatgccgc
     2941 gaaggcgctc ttcaagaccg gcaagctcta cgaggacctt caccggttct cctcgaggcc
     3001 cgacgacctc gaaaaggctg cggacacgta cctggcgctg gccgagaagt acccgaagaa
     3061 ccagctcgcc gacgacgccg tctacagcgc cgcacgcatc caccacctgt cgctcggatc
     3121 ccccgtgaag gcgtcgtccc tctaccagag ggtcatccgt gattaccccg agggcgacat
     3181 ggtgcagctc gcccgcgagc agatgaacgc gctcaacgtc gagaagagaa cggtcacggc
     3241 accgagcttg ggcgggcccg gcaaggtgag gtcgctcaac cagtggtccg acagggacta
     3301 cacccgcgtt gtcgtcgagc tcgacgacaa cgtcaccttc aagaccttca ccctgcctgc
     3361 cgacgcctct gcgaacaggc cggagcgggt gatcctcgag ctcgacaacg cgaggatctc
     3421 accctccatc ccggctctga cccgcgttga cgacgggatc ctcaagaaca tcagggcctc
     3481 gcagttctcc gccgacaagg tgaggatcgt gctcgacctc gccaggaagg tcacctatcg
     3541 ggctttcccc ctttccgatc catcgaggat ggtggtggac atcgtgaaga agggggcgcc
     3601 catgtctgtc gcggccggag attcacggca gaagacgccc gccgagcctc tgggcatgag
     3661 caccggcggc gtccagaggc ccgaggcgaa gaacgggttc aagaaggtgc ccaagtggtc
     3721 tcaatcgcag atgtccgacg acgtcccgtc catagccgca cagctctcgc tgaaggtctc
     3781 ccgcatcgtg gttgacccgg gccacggcgg gaaggacccg ggggcaatcg gcccgggggg
     3841 tgtcaaggag aaggacctca ccctcgccat cggcaaggcc ctcgccagga ggctcaggca
     3901 cgaggggttc gaggtgttcc tgacgaggga gacggacgtg ttcatccccc tcgaggagcg
     3961 caccgcgttc gccaacaaga agcgggccga cctgttcgtc tccatccaca tcaacgccca
     4021 caacaacacc tcgctccagg gtatcgagac ctacttcctc aacctcacca cggacgcctc
     4081 ggccatagag gtggcggccc gcgagaacgc caccacgagc aaatccatca gcgacctcca
     4141 gctcatcatc aacgacctga tgctcaactc caagatcaac gagtcgtcga ggttcgccac
     4201 ctcggtccag aaatgcatca tgtcgtcgct catcaacgat ggctaccagg gcaaggacct
     4261 aggggtcaag caggcaccgt tctatgtcct cctgggcacc cagatgccct ccatactctg
     4321 cgagctcggc ttcctcacca actgcaccga agcggacctc ctccagaacg aagcgcacca
     4381 gaacgccctc gtcgacggta ttgccaaggg tatcaacaag tatataatga acaccacgta
     4441 cgcctacttg aggagcgcca agtgaggggt ttcatcctaa cggcggcggt gttcgtcctg
     4501 tgcgcggtct gtccagccgc gtgggcaggc tacggcctga gcaacgtggt ggaaaagacc
     4561 atcgcgcagc cagactcttc ccaggcgcct cccacgtcaa agccccaggc gcaggaggct
     4621 ccaccggcac agccgggtgc gaagcaggcc ccccaggaag ccaagcccgc ctccaccgcg
     4681 acggccccgg aacccctgcc gacacccgcg aagaagccgg ccatcaccta cgagaagacg
     4741 gccttctacc cgtacacgat acacatctcg tcctggcagt cgaagaagga cgcgctcaac
     4801 cacatgcaga ggctctcggg cgagctcgag ggggtgttcg tgaccaagat cgacctgggg
     4861 ccgaccgggc tctggtaccg ggtcgactac ggcgtcttcg agaacgacca gcaggccggc
     4921 caaacaatgg ccgagctcaa gaccaagggc gtcctgcgca acgacccgtt catcggttcc
     4981 cccgtgccgt acgccataga gataggcgtc tttccgagca aagacgccgc ctcggcagag
     5041 gcctcgaggc ttatcaggaa aggggtgtcc acctacgtgg tcaaggagtc ggaagccgcc
     5101 tacaggctcc tgtcgggcgc gtaccacgac aggaagagcg cggagccggc gctcgcagac
     5161 ctcgccgcgc tgggcatacg ggcaaagaca gccaagagat gaaccgctcg ctcgtcgact
     5221 ccttcctgga gtacctcgtg gctgagcggt cttcccctga gaacaccctg agggcctatg
     5281 ccttagacgt ggccgactac ctctcttttc tcgaatccag gggcatcgcc gccgccgatg
     5341 ccgaggcctg gaccctggag gattacgccg tgcacctgag ggcctccggg ttgaaggcaa
     5401 cgagcgtgac caggaaactg agcgcgatca ggcacttcta ccgcttcctc ctggaggagg
     5461 atgcggccga gcgcgacccg accaaggacg tggcctcgcc gaagagggtg cgcaccctgc
     5521 ccgaggtgct ctcaagggag gaggtggagc ggctcgtgga gagccccgac tgttccaccc
     5581 cccagggcgt gagggaccgg gccatgctgg aggtcctcta tgccacgggc ctcagggtga
     5641 gcgagctggt gggactcagg ctccacgatg tcgacctcgc cgtggggtat gtcctctcga
     5701 ggggcaaggg cgacaaggag cggatcgttc ccctcggcga gagcgcgagg cgctggctcg
     5761 cccggtacgt cggcgaagcg aggccccggc tcgtgaagaa gtcgacggac gtcctgttct
     5821 gctcgcgcct ggggaaggcc atgaccaggc agaacgtgtg gcacgcgatc aagcgccacg
     5881 ccaggcatgc aggcatcgcg aaacggatat ccccgcacac gctcaggcac tcgttcgcca
     5941 cgcacctgct cgtgggtgga gcggacctca ggtcggtcca ggagatgctc gggcacgcgg
     6001 acatctccac cacccaggtc tacacgcacg tgacgagccc gaggctcaag gagatccacc
     6061 ggaggtacca cccgagggga taggacatgg aggtcatcac ctcacacatc caggcggact
     6121 tcgacgcctt cgcgtccatg ctcgcggccc gcatcctctt cccggaggcg ctgctcgtgt
     6181 tccccggggc ccaggagaag aacctgaggg agttcgtctc ccagctggat cccggccttt
     6241 cgggcgcgat cgcgaagctc aagtccatcg atctcgagag cgtgagacgc ctcgtcatcg
     6301 tcgacaccag gcagctctcg cgcatcgggg acttcgcgag gatagtgggg agaccgcacg
     6361 tggaggtgat cacctacgat caccacccac cctcgaacga cgacatcgtg accaaagaca
     6421 gccacgtcag ggagtacggc tccaacagca ccctcatggt cgaggaactg aagggccgcg
     6481 gcctcgtacc cccaagcgag gtggcgacga tcctcgccat ggggatcttc gaggacacgg
     6541 gttcgctcct gttcccgtcc accaggcccg aggactgcat ggcgctcgcg caactcctcc
     6601 agtggggggc ggacctcacc cacgtgtcga ggagcctcgc gcgcgacctc agcccggacc
     6661 aggtggacgt gctcgaccag ctcatcaaga acgcggccat tcaccacgtg gggggcatgg
     6721 acgtgctcgt gacgacgtcc gagtccacgg gctacatcga ggacttcgcc atgctcgtcc
     6781 acaagctcag gggcatgttc caggtcgatg cggtgttcgc cgccgggctc atgggcgagc
     6841 ggatctacct ggtggcgagg agcaccgtgc cccagatcaa cgccgcggag atagcggccc
     6901 ggttcggcgg cggcgggcac ccgacagcgg ccgcggcctc catcagcggc cgatccctcc
     6961 agagcgtggc cggcacgttg gtgaccatca tcaagaagga gacgaggccg cggatcacgg
     7021 cgcgggacat catgaccttc ccggtcatat ccctcaagat gaacgccacc attgacgagg
     7081 cggcccagac cctgaaccgg tacaacataa acgcggcggt cgtccagtcg agggatcaca
     7141 aggccgtggg cctggtgaca cgccaggtgg tcacgagggc acagggccac ggcctgggag
     7201 gggctccggt caaggactac atgatccgcg acatccagtc ggtgggccct gaggcgccgg
     7261 tgggggagat ccggaccctc atcatcgacg gcaaccagcg gctcctgccc gtgatcgaga
     7321 acaagaaggc gataggcgtc atcacgagga ccgacctcat gcgagtgatg cacgagaaga
     7381 tggccaagac cgagggtatc gacctcaagg cctcccagag aaagaacgtg gcgtcgctca
     7441 tggcggaacg cctgcccagg gagatcgtgg agatgctgcg cgacgccggg atccaggcgt
     7501 cctcgatggg gttcaacgtc tatctcgtgg gcgggatcgt ccgcgacatg gtcatgcgca
     7561 tcgacaacct cgacatcgac ctcgtggtgg agggcgacgg catcgccttc gcgagggcct
     7621 tctcgtccaa ggtcggcatc aggtgcgccg tccacgagaa gtacaagacc gcggtcatca
     7681 ccctgcccga cgggaggcac atcgacgtgg ccacggccag gaccgagtat tacaagcacc
     7741 cgggtgggta cccggtggtg gagcaggcca cgctcaagct cgacctgtac aggagggact
     7801 tcaccatcaa caccatggcg gtggcgctcg tcccccagcg tttcggcgag ctcatcgact
     7861 tcttcggggc ccagcgggac ataaaggaca ggcgcatcag ggtcctccac gccttgagct
     7921 tcgttgagga cccctcgcgc atcctgcggg cggtgaggtt cgagaagcgc tacgggttca
     7981 cgctcggcaa gcagaccctc gcgctcgttc acgccgcggt gcgctcgggg ctcttgagca
     8041 gcatcccggg gaggcgcatc tcccacgaga tccgacagat cctctcggaa gaagatcccg
     8101 ttgcctgcct cgagaggctc aaggaactcg acgtcctgga ggcggtgcac ccggacctca
     8161 cgttcacccc cgaggtgagg gagatcatgc tctccgtgaa acagaccatc tcctggttcg
     8221 agctcctcta tacgcacgaa ccctacaggc cgtggttcat ctacctcctc ggcctggttg
     8281 acaccatgaa accgaaaaag atcgtcgaga tctgccagcg gctggggctc acgccggaaa
     8341 acatccgcac gatcctgtcc tcgcgatcga gggtcaacgc cgtgctcagg gacctggcca
     8401 ccgacggcga gaagaggcct tcgaacgtcg cg
//
LOCUS       DAOSCR010000029         8304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5603_length_8304_cov_3.169596, whole genome shotgun sequence.
ACCESSION   DAOSCR010000029 DAOSCR010000000
VERSION     DAOSCR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5603_length_8304_cov_3.169596"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1212
                     /locus_tag="PLT69_01515"
     CDS             136..1212
                     /locus_tag="PLT69_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOJ13161.1"
                     /translation="MSNTTKELLRRKIYEGRRIMPEEALDLFSWDLPELGMAADFRRR
                     MAVPGDTVGFIVDRIINFTNVCEAACAFCAFHAKPGGPGRYESSMDEICGKVEELVIN
                     GGTQVMLQGGLHPDYTLETYVNMVRTIKGRFPEVVLHSFSPAEIVHMARKGSLSLDQV
                     VEALKEAGLDSVPGASDILVDRVRSRVCPNKLTAAQWRSVMHCLARHGMKSSATMTYG
                     LGETMRERVEHLGVIREVQDQTGIIRAFIPWSFSSARTSLADMPPSTGLDYLRIVAVS
                     RIFLDNIPHIQAGWLTEGTKLAQVALSMGADDMGGVLTEEVVVRATGIATRTNVDEMI
                     HLILDAGKTAVQRDSDYREIRRFG"
     gene            1222..2007
                     /locus_tag="PLT69_01520"
     CDS             1222..2007
                     /locus_tag="PLT69_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOJ13162.1"
                     /translation="MDIAFTPCPNDTFVFHALVHGLIDTSPLVFTPVMRDVEALNRDA
                     FHSRYGVTKLSVHAVLKLDDRYEILSSGAAIGRGCGPLVVSCKPGIRLDEARIAVPGG
                     HTTAALLLKLWNPGLRTVAVLRYDEILPAVAGGAFDAGLIIHESRFVYPEYGCEQVVD
                     LGRWWEDETGLPVPLGCMAVRRDLSGSKGRIEELVRESIRHAQADPCATMEYVRSHAQ
                     ETRQDVIDSHISLYVNEYSIDMGEEGRRAIEVLRDRARLKGVL"
     gene            2004..2627
                     /locus_tag="PLT69_01525"
     CDS             2004..2627
                     /locus_tag="PLT69_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13163.1"
                     /translation="MIGIAMATMLEAEPFITGLGLKEVDRSPFHVFTSGHTVLVVTGM
                     GKINAALGVAHLATRHGVSTVCNIGAAGALSPDAGIGEIFQVREVVEPDRLHVATGEI
                     MRSTPSIVDGIPEAVLATMDRPIRETSERRAMSRLAGLVDMEGAGICQASRRYGLPCH
                     LVKFVSDTSPGCDIAWNIGLLRDTVFAFCRDTFLWKLGWSGPTRPGA"
     gene            2660..3529
                     /locus_tag="PLT69_01530"
     CDS             2660..3529
                     /locus_tag="PLT69_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOJ13164.1"
                     /translation="MMLSRIRTYGRMIKFSHTVFALPFALSAAVLVHQERPLTPATVF
                     WILVAMVSARSAAMGFNRIADADLDARNPRTANREIPLGVISRRWAGAFVAVSAALFV
                     GAAAMLSRLCFVLSIPVLAVLFAYSYTKRFTWLSHIVLGFAIGMAPTAVWVAASGTIT
                     FRICVMSVALLTYIAGFDILYACQDVDFDRREGLHSIPQRFGIQGALKISRLLHAVSV
                     ACLAGMYELFSLHVVYLFFVAAIAVLFVVEHLLVRKDDLSRIDIAFFNVNSAISVLVL
                     AAIVAGETAKRWT"
     gene            3526..4137
                     /locus_tag="PLT69_01535"
     CDS             3526..4137
                     /locus_tag="PLT69_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOJ13165.1"
                     /translation="MTERFIVGITGASGTIYGVRLLQALLACSCEIHLIVSDMGAKVA
                     SHDLCLPEEGIENALASLAAGGSAPGRIMLHNNRDMFAPVASGSFQVRGMAVIPCSMK
                     TMAAVASGYTSGLLERAADVTLKERRTLVLVPRETPLSRVHLKNMLAAQEAGAIILPA
                     SPAFYHHPETVDDLVDFVVERVMDTLGVPMPRARRWGTQGRKG"
     gene            4142..5968
                     /locus_tag="PLT69_01540"
     CDS             4142..5968
                     /locus_tag="PLT69_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOJ13166.1"
                     /translation="MSFRNLGEFIACLEEAGELERIRKPVSPRLEITEITDRMCKSSG
                     GGKALLFENVAGSVFPVLTNAFGSMRRIGLALGSTPDALAERLREILELTPPASFLEK
                     LSMAGKAVSWSRFLPRLSGTKRPPCREVVYRGGEVNLHRLPVLHCWPEDGGPFVTLPV
                     VFTKGLSDGKRNVGMYRMQVYDRNTTGMHWHVHKDGAHHFKEYREAGRRMEVAVAIGT
                     DPAVTYAATAPMPRGVDEMILAGFIRRKPVTMVKCLTVDLEVPAESEFVLEGYVDPGE
                     TRIEGPFGDHTGYYSLADEYPVFHVTAVTHRANPVYSATVVGRPPMEDCYLAYVTERL
                     FLPMIRTMLPEIRDYWLPWEGVFHNIVVVAMEKAFPGHASKVMSGLWGMGQMSFAKMI
                     VTVDDESLLRKGGALLAHILDTVDPWTDLTFTHGILDVLDHSAPQGLHGLKVGIDATF
                     RVAGEMPRTRRERMAPPMGGDGILRRLRATDSGFVSVRKLFEERVHPLVLVGVAKGGA
                     RSSRSFLERIPQDVLGQGIVVLYDDGVDLTDGSLVLWKAFNNVDPARDIHVHPAGVVV
                     DATRKGPMDGHMRTWPDEIRMTEEIRALVDEKFKDLFVSSGP"
     gene            complement(6191..7228)
                     /gene="cydB"
                     /locus_tag="PLT69_01545"
     CDS             complement(6191..7228)
                     /gene="cydB"
                     /locus_tag="PLT69_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOJ13167.1"
                     /translation="MDALYALQVTWFVLWGVLWSVYFMLDGFVLGMGMLYPFMARDEK
                     EKRTMIATVGPVWDGNEVWLITAGGATFAAFPTTYALMFSYLYTALLLLLFSLIIRGV
                     SIEFGGKVEKQGWKRFWDVGIFVGGMLPAVLFGIAFGNIFQGLPMDASGYHGTLVGLF
                     NPYGLLAGVLFVFLFMVHGALYVSVKTTGGLSRRAAAFAGRSWSVLAAVAILFLVHTY
                     LSTGIFANYLASPVLFLLPLAAVSSLALIRLQLSRGSLAGAFAASCSTIVLVCATGFV
                     GLFPNLIPSSLDTAYSMTITNSSSSVATLAVMTVVALVFVPVVIAYKAWVYRVFRGPL
                     NPEDIDSQKVY"
     gene            complement(7230..>8304)
                     /locus_tag="PLT69_01550"
     CDS             complement(7230..>8304)
                     /locus_tag="PLT69_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOJ13168.1"
                     /translation="SAFFMESVFLGLWMFGWNKVSKKTHAWSITLVAFATNLSALWIL
                     LANGWMQNPVGANIVELAGNPLVSRRAEMADVFAVFTNPYGWVKFFHTVLSGYVLAAF
                     FVMGVSAWHLLKPNEVVLFKRSFKAAAVFGLVSGFLVLGTGDFHGSEIAKTQPSKLAA
                     LEAVWETHKGVGLSIVVIPDSEAERNSLELITIPKLASLLAYRDPDAEIKGLKDIPKD
                     LRPPVGLVFHSFRLMVGIGMLMLLLSIAAVYLSFRDRIEASPLFLKLMIPAMALPYIA
                     GQLGWIVAEVGRQPWIVYGVLKTADAASKSVDAAQVLFSLVAFTVVYSLLGAVDAYLL
                     VTSAKAGPEARLPAGLDATGKGV"
BASE COUNT         1571 a         2534 c         2773 g         1426 t
ORIGIN      
        1 cagggagggg ctcgggtact accttgcagt tgcgggcgag atggggttcc tgcccgagac
       61 gagggtgata cggtatgcag cgtacgtgga caccgagggc ggcgtcgaag accgcctgga
      121 tgcgggcgcg cgcctatgag caacaccacg aaggaactcc tcaggcggaa gatatacgag
      181 gggaggcgta tcatgcccga agaagccctc gacctcttct cgtgggacct gcccgaactc
      241 gggatggccg ccgatttccg aaggcgcatg gccgtgcccg gggacacggt cggcttcatc
      301 gtggaccgga taatcaactt cacgaacgtc tgcgaggccg catgcgcctt ctgcgccttc
      361 catgcgaaac cgggcgggcc cggcaggtac gaatcgagca tggacgagat ctgcggcaag
      421 gttgaagaac tggtcatcaa cggtgggaca caggtgatgc tccagggggg actgcacccc
      481 gactacaccc tggagaccta cgtgaacatg gtgaggacga tcaaggggcg tttcccggaa
      541 gtcgtcctgc actccttctc gcccgcagag atcgtgcaca tggcgaggaa gggttccctg
      601 tccctggacc aggtcgtgga ggccctcaag gaagcggggc tcgactcggt gccgggggcg
      661 tcggacatcc tcgtggacag ggtccgctcc cgcgtatgcc cgaacaagct tacagcggcc
      721 cagtggcgct cggtcatgca ctgcctcgcc cgccacggca tgaagtcgag cgcaaccatg
      781 acctatgggc tcggtgagac catgcgggaa cgcgtcgagc acctgggtgt gatccgtgag
      841 gtacaggacc aaaccggtat cataagggcc ttcattccct ggtcgttctc cagtgcgcgc
      901 acaagccttg cggacatgcc gccgagcacc ggcctcgact atctcaggat cgtcgcggtc
      961 tccaggatct ttctggacaa catcccccac atccaggcag gatggctgac agaaggcacc
     1021 aagctcgccc aggtggccct gtccatgggg gcggacgaca tggggggcgt gctcacggag
     1081 gaggtggtgg tccgcgcgac cgggatcgcc acacggacga acgttgacga aatgatccac
     1141 ctcattctcg atgcagggaa gacggctgtg caaagggact cggattaccg ggagatcagg
     1201 aggttcgggt aagccttgca cgtggatatc gcctttaccc catgccccaa cgacacgttc
     1261 gtcttccatg cgctggtgca cggcctcatc gacacgtcgc cgctcgtgtt cacccctgtc
     1321 atgagggacg tggaagccct gaacagggat gcctttcact cacgctacgg cgtcacgaag
     1381 ctctcggtac acgccgtgct caagctcgat gaccgctacg agatcctctc gagcggtgcc
     1441 gccatcggcc gcggctgcgg ccctctcgtc gtctcctgca agccggggat acgcctggac
     1501 gaggcccgca tagcggtgcc tggaggccat acgacggcag ccctcctcct gaagctgtgg
     1561 aaccccggac tgagaaccgt ggccgttctc aggtacgacg agatcctgcc ggcagtggcg
     1621 ggaggcgcgt tcgatgcagg gttgatcatc cacgaaagca ggttcgtata ccctgagtac
     1681 ggttgcgagc aggtcgtgga cctgggcagg tggtgggaag acgagacggg gctgcccgtg
     1741 ccgcttggct gcatggctgt gcgcagagac ctctcgggtt ccaaggggcg catagaggaa
     1801 ctcgtcaggg aatccatccg ccatgcgcag gccgacccct gcgcaaccat ggagtacgtg
     1861 cgttcgcacg cacaggagac caggcaagac gtcatcgaca gccacatttc cctgtacgtg
     1921 aacgaataca gcatcgacat gggggaggag ggacgaaggg ccatcgaggt gttgagggat
     1981 cgtgcaaggc tgaagggggt cctgtgatcg ggatagccat ggctaccatg ctggaagcgg
     2041 agccgttcat caccgggctt ggcctcaagg aggtcgaccg ttcgccgttt catgtcttca
     2101 cgtcggggca tacggtcctc gtcgtgacgg gcatgggaaa gatcaacgcc gccttggggg
     2161 tggcgcatct cgcgacgagg cacggggttt ccaccgtgtg caacatcggg gcggcgggtg
     2221 cactgtcgcc ggatgccggg attggcgaga tcttccaggt gagggaggtg gtcgagcccg
     2281 accggttgca cgtcgcaacg ggagagatca tgagatccac gccaagcatt gtcgacggga
     2341 tacctgaggc cgtcttggcc accatggaca ggccgatcag ggagacgtcc gaacgcaggg
     2401 ccatgtcgag gctcgctggt ctcgtggaca tggaaggcgc cggcatatgc caggcgagca
     2461 ggaggtatgg cctgccctgt cacctggtga agtttgtcag cgacacgagc ccgggctgcg
     2521 acatcgcatg gaacatcggc ctcttgaggg acacggtgtt cgccttttgc agggacacgt
     2581 tcctttggaa gttgggctgg tcgggaccga caaggcctgg tgcttgacaa ggcggggcgc
     2641 gagcggctag gtcctcatca tgatgctctc gaggatacgt acctacggca ggatgatcaa
     2701 gttctcccac accgtgttcg ccctgccttt cgccctgtct gccgcggttc tcgtccacca
     2761 ggagaggccc ctgacccctg ccaccgtgtt ctggatactc gtggccatgg tatcggcgcg
     2821 gtctgccgca atgggtttca accgcatagc agacgcggac ctcgatgcga gaaacccgcg
     2881 cacggcgaac agggagatcc ccctgggggt catctcgcgg aggtgggccg gtgctttcgt
     2941 ggcggtatcc gccgcccttt tcgtcggcgc ggccgccatg ctctcgaggc tctgcttcgt
     3001 gctctccatc ccggtgcttg ccgtgctgtt cgcctactcg tacacgaaac gcttcacctg
     3061 gctgtcccac atcgtgctgg gctttgccat aggcatggcc ccgacggccg tctgggtggc
     3121 ggcgagcggc accatcacct tcaggatctg cgtgatgagc gtcgcgctgc tcacgtacat
     3181 cgccgggttc gacatcctgt acgcatgcca ggatgtggac ttcgaccgcc gggaaggcct
     3241 gcactcgatc ccgcagaggt tcgggatcca aggcgcgctc aagatctcca ggttgttgca
     3301 tgcggtgagt gtggcgtgcc ttgcgggcat gtacgagctc ttctccctcc acgtggtcta
     3361 cctgttcttc gtggcggcga tcgccgtgct cttcgtggtg gaacacctcc tggttcgaaa
     3421 ggatgacctc agcaggatcg acatcgcgtt cttcaacgtg aacagcgcca tatcggtact
     3481 cgtcctcgcg gccattgtgg cgggcgagac ggcgaagagg tggacgtgac ggaacgtttc
     3541 atcgtgggga taaccggggc atcgggcacc atttacgggg tgagactcct gcaggccctc
     3601 cttgcatgct cgtgcgagat ccacctcatc gtctccgaca tgggggccaa ggttgcctcc
     3661 cacgatctct gcctgcccga ggaaggcatc gaaaacgccc ttgcgtccct ggctgccggt
     3721 gggtcggcac ccgggagaat catgctccac aacaaccggg acatgttcgc gcctgttgcg
     3781 agcgggtcct tccaggtacg cggcatggct gtcatcccgt gctcgatgaa gaccatggcc
     3841 gccgtggcgt cagggtacac ctccggcctc ctggaacgcg ccgcggacgt gaccctcaag
     3901 gagcgcagga ccctggtcct tgtgccccgg gagacgcccc tgtcacgggt tcacctgaag
     3961 aacatgctcg ccgcacaaga agccggggca atcatcctcc cggcatcccc ggccttctac
     4021 catcaccccg agaccgtcga tgacctggtc gacttcgtgg tggagcgcgt gatggacacg
     4081 ttgggtgtcc ccatgccgcg tgcgaggcgg tggggaaccc agggccgcaa ggggtgagcc
     4141 gttgagcttc aggaatctcg gggagttcat tgcgtgcctc gaggaggcgg gcgagctcga
     4201 acgcatacgg aagcccgtct cgccccgcct cgagatcacc gagatcacgg accgaatgtg
     4261 caagagcagc gggggcggca aggccctgct cttcgagaac gtcgccggct cggtcttccc
     4321 tgtgctcacg aacgccttcg ggagcatgcg aaggatcggt ctcgcgctcg gctccacccc
     4381 ggatgcgctt gccgagaggc tcagggagat cctcgaactc acacctcccg cgagcttcct
     4441 ggagaagctc tcgatggccg gcaaggcggt ttcctggtcg aggttcctgc ccaggctcag
     4501 cggtacgaag cgcccaccct gccgggaggt ggtctacagg ggaggcgagg taaacctcca
     4561 caggctgcct gtgctccact gctggcccga agacggtggc ccgttcgtga cgctgcccgt
     4621 ggttttcaca aaggggctct ccgacgggaa gcggaacgtg ggcatgtaca ggatgcaggt
     4681 ctacgacagg aacaccaccg gcatgcactg gcacgtccac aaggatggcg cccaccactt
     4741 caaggagtac cgggaggcgg gcaggcgcat ggaggtggcg gtggccatcg gtactgaccc
     4801 cgcggtcacc tacgcagcaa cggcgcccat gccccggggc gtcgacgaga tgatcctcgc
     4861 cgggttcatc cgcaggaagc cggtgaccat ggtgaagtgc ctcaccgtgg acctcgaggt
     4921 gccggcggag tcggagttcg ttctcgaggg atacgttgac ccaggcgaaa cgaggattga
     4981 aggccccttc ggcgaccata ccggttatta cagcctggcc gacgagtacc ccgtgttcca
     5041 cgtgaccgct gtcacccaca gggccaaccc cgtctacagc gccacggtgg tgggcaggcc
     5101 gccgatggag gactgctacc tcgcctacgt gacggaacgc ctgttcctgc ccatgatcag
     5161 gaccatgctg cccgagatca gagactactg gcttccctgg gaaggggtat tccataacat
     5221 tgtcgtcgtc gccatggaga aggcattccc cggccacgca tccaaggtga tgagcggcct
     5281 gtggggcatg ggccagatga gcttcgccaa gatgatcgtg acggtggacg acgagagcct
     5341 gctcaggaag ggcggcgcgt tgctggcgca tatcctggac accgtcgacc cgtggacgga
     5401 cctcaccttt acccatggta tcctcgacgt gctggatcac tcggcgcccc aggggctcca
     5461 cgggttgaaa gtggggattg acgccacgtt ccgtgtggcg ggggaaatgc cgagaacgcg
     5521 cagggagcgc atggcgcccc ccatgggagg ggatgggatc ctgagacgcc tgagggccac
     5581 cgacagcggg ttcgtgtcgg taaggaaact cttcgaggag cgagtgcacc cgctcgtgct
     5641 tgtcggcgtg gcgaaggggg gggccaggag ctcgaggtcg ttcctggaac ggatacccca
     5701 ggatgtcctc ggccagggca tcgttgtcct gtacgacgac ggggtcgatc tcacggacgg
     5761 gtccctggtc ttgtggaagg cgttcaacaa cgtggacccc gcccgggaca tccacgtcca
     5821 cccagcaggc gtggtggtcg atgccacgag gaaagggccc atggatgggc acatgcgcac
     5881 gtggcctgac gagatccgca tgaccgagga gatccgggcc ctggtggacg agaagttcaa
     5941 ggacctcttt gtgtcaagcg gcccctaggg cggtatcgtg cccattcgcg gtcccatcgc
     6001 gagcgtcccc ccctccgggc cgatcgtcct ctttgggccc gggtggggta tgcccgaggc
     6061 cgtgccgcgc cgttttaggc ggagaacggg cacgcagctc aatcggccac cgagcgcctc
     6121 aggtgggcat gccctgtccc gaccacggtg aaagagggcg gtcccgtctc cttcgtgaac
     6181 cggcacaggg tcagtagacc ttttgcgagt cgatatcctc cgggttcaat ggccccctga
     6241 agaccctgta cacccaggcc ttgtacgcga tcaccacggg gacgaagacg agcgccacca
     6301 cggtcatcac ggcgagcgtc gccacgctcg aggacgagtt cgtgatggtc atgctgtagg
     6361 ccgtatccag gctcgacggg atcaggttgg ggaacaggcc gacgaagccc gtggcgcaga
     6421 cgagcacgat ggtcgagcac gaggcggcga aagcgcctgc gaggcttccc ctcgaaagct
     6481 ggagccttat gagcgccagg cttgagacgg cggccagggg gagaaggaac aggacgggcg
     6541 aggcgaggta gttggcgaag atgcctgtcg aaaggtaggt gtggaccagg aacaggatcg
     6601 ccacggccgc aaggacgctc cacgacctcc cggcaaaggc cgcagccctc ctgctcaggc
     6661 cgccggtcgt cttgacggac acgtacagcg caccgtgcac catgaacagg aacacgaaaa
     6721 gcacgccagc aagcagcccg tacgggttga acaggccaac gagggtgccg tggtagccgg
     6781 aagcgtccat ggggaggccc tggaagatgt tcccgaaggc tatgccgaag agcacggcgg
     6841 gcagcatgcc gcccacgaat atgcccacgt cccagaagcg cttccatcct tgtttctcga
     6901 ccttgccgcc aaactcgatg gagacacccc tgatgatgag agagaagagg agcaggagca
     6961 gggccgtgta caggtagctg aacatgaggg cgtacgtggt ggggaaggca gcgaacgtgg
     7021 cgcctcccgc ggtgatgagc cacacctcgt tgccgtccca taccgggccg accgtggcga
     7081 tcatggtccg tttctctttc tcgtccctgg ccatgaacgg gtacagcatg cccatgccga
     7141 gcacgaaccc gtcgagcatg aagtagaccg accagagcac gccccagagc acgaaccacg
     7201 tcacctggag ggcgtacagg gcatccatct cacaccccct ttccggttgc gtcgaggccg
     7261 gcagggagcc ttgcctccgg gcccgccttc gcagaggtga cgaggagata ggcgtccaca
     7321 gcgccgagca ggctgtagac cacggtgaag gcgacaaggg agaagaggac ctgggccgca
     7381 tccacggact tggacgccgc gtctgcggtc ttgagaacgc cgtagacgat ccacggctgc
     7441 ctgccgacct cggcgacgat ccacccgagc tggccggcga tataggggag ggccatggcc
     7501 gggatcatca gcttgaggaa caggggagac gcctcgatcc ggtccctgaa ggagaggtac
     7561 acggccgcaa tggacaacag cagcatgagc atgccgatgc cgaccatgag gcggaaactg
     7621 tggaagacga gccccacggg cggcctgagg tctttgggga tgtctttgag acccttgatc
     7681 tcggcgtcag gatcccggta cgccagcagg ctcgcgagct tcggtatggt gatcaattcc
     7741 agggagttgc gttccgcctc gctgtcgggt atcacgacga tgctcaggcc cacgcccttg
     7801 tgggtttccc acaccgcctc gagggccgcc agcttggaag gctgcgtctt ggcgatctca
     7861 gacccgtgga agtccccggt tcccaggacg aggaagccgg agacgagccc gaagacggcg
     7921 gccgccttga acgaccgttt gaacaggacg acctcgttgg gcttcaagag gtgccaggcc
     7981 gagactccca tgacgaagaa ggccgcgagg acatagccgg acaggaccgt atggaagaac
     8041 tttacccagc cgtaggggtt ggtgaagacc gcgaagacat ccgccatctc ggcccgccgg
     8101 gaaacgagcg ggttgcccgc gagctctacg atgttggcgc ccacggggtt ctgcatccag
     8161 ccgttggcga gcagtatcca cagtgccgag aggttcgtgg cgaaggcaac cagcgtgatg
     8221 gaccaggcgt gggtcttctt cgagaccttg ttccagccga acatccagag gcccaggaag
     8281 accgactcca tgaagaaggc cgat
//
LOCUS       DAOSCR010000030         7976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5881_length_7976_cov_3.643984, whole genome shotgun sequence.
ACCESSION   DAOSCR010000030 DAOSCR010000000
VERSION     DAOSCR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5881_length_7976_cov_3.643984"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PLT69_01555"
     CDS             <1..1382
                     /locus_tag="PLT69_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13169.1"
                     /translation="YSESATVSPDNRHIALVAKKNDRFALIVDGKMHKEYEAISKDTP
                     PRFSPDSRHVAYKVKKGRKWLVVLDGDELKPYDNVGGITFSPDSRRLAYVAQKGADYI
                     VVLDGVESKAYDGIPHTNNPVFSPDSKRLAFLALKGQKMVVVEGGQEGKEYEKISYPF
                     FSPDSKHLVYPVIDKKKMFIVFDGKELPQYEKISLQAFSPDSTRFVYTARQGDKSFMV
                     VDGKPQQPFTDLALPVFSPDSKRLAYLAKEGEKFVMVIDGVKTAPFDEVLQFGFSEDS
                     SRYFFTARKGDTWSVYTDGKPQGAYDAVLLPMFSPDSRRLAYAAMKGKKWTLVVDGKQ
                     TAFYDAILDIQFSPNSQHVLCRAMKDNKWKAILDGRESSTYDVVVLPMFSPDSRHHAF
                     LASEKGKWFAVIDGKEGKERFLMFLKGAQQQAVNLSIDNEGRLSLVAIQENKDSTLGP
                     NFVRYTVDM"
     gene            1433..2185
                     /locus_tag="PLT69_01560"
     CDS             1433..2185
                     /locus_tag="PLT69_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ13170.1"
                     /translation="MYGSSRVVVVMPAYQAEHTLRMTYDEVMAQGVVDTVIVVDDNST
                     DRTVEIAMTLPDTIVHRHERNRGYGANQKSCYRLALEVGADIVIMVHPDYQYTPKLIP
                     AMASLIGNGLYHCVLGSRILGGYALKGGMPLWRYVANRFLTLAGNILMSAKLSEYHTG
                     YRAFSRELLERVPFERNSDDFVFDNQMLAQILWYGYTIAEVSCPTNYFEEASSINFAR
                     SVKYGFGCLHTALAFRLARHGIVRSELFPEPS"
     gene            complement(2539..4944)
                     /locus_tag="PLT69_01565"
     CDS             complement(2539..4944)
                     /locus_tag="PLT69_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ13171.1"
                     /translation="MISRALRIALAALLAAASLAAVILRYAEPVTDGDIWFHLAYGRY
                     MLENTTLRPDHAAFSWTQADASPIYCAWIPQVLFFKIHEALGMPGLLALRYLFVCAFL
                     ALAALMAAPALRRKPGLSPLVILVCLTGLLMSQAGLLVKAELFSFLFTAFLSFLWFSS
                     KTGGAWSRASILAIPLLVLAWVNSHGGFIFGVFFIACATGGEVLNALFRSPARLKADL
                     VPTALVALGLSAAAILFTPYGWDYPAYLYETLFSETFSRHTASVRAYYSILAPEVRGT
                     HYLEYLAAALVVTIGLVVAAARRRTLDWSTVTIGIGFALLFLRYMRTTYFFGVVFVFT
                     AISLMRRITESAPAGGGHAGSRLTPSRAGKPLDTGGSGRIGPGVSSPAKKTGGVMPGD
                     EGTARHAAYKTIAVQAAASLLVLFFSARMGVESLFSPQAGLNVLHFSDPAGEASFVRN
                     NLEGLVMGNTYNCGSYLIWSLWPTHKVFIDERYFPYAGWYAEYNAFEYGPDDAYRHAF
                     LQRYRCDFWVLSLDFPHMRFFLSSPDWRLVHYGPKAAVFLSSRLAPAGTGHTVSDTVY
                     RTGFYNALAASDFATTVMDLDVASRILTGIEPSPFWPEQSRRLGESLERLGAAYAKAG
                     RADNAVEVLRRATTLNPSSTRAHLFLGNALVLAGRPNDATEEYSTVLELDPGNEDARR
                     SIAAIGALSGAQAGVRGHAGLDEDGFAALASSARRKARSGDYEGALKELECMLEIRPG
                     DPETLYNVSCALARLGRLDEAMDRLEEAVSRGFDRWDLMKSDPDLASLRETDRYRSLV
                     RGR"
     gene            complement(5695..6681)
                     /locus_tag="PLT69_01570"
     CDS             complement(5695..6681)
                     /locus_tag="PLT69_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13172.1"
                     /translation="MQRYLLILMMLLCMPLSLWAMTPVADDELSLVTGQAGVNINADV
                     TMNIAIGTMAWGDASGIDTGFAQWGWGTADTTAGYVGVTNFNISGLRVAARENPLDNF
                     NGYTTTMLKPITIDVGSGLSHGGATFVRFGLGSLEIRMNSMTMNVALGTAGASLSQVL
                     GTVNIASMIMYINPSSYVDIYTAGGVGVNFFMNVIIDQFSMGRLAWGDTDGAGSNVGA
                     GGVPWFVNSTAGYIGLDNLVVGGPISISGSVRIDVGTVNTGVYAAHGPTTVVHMVFGD
                     NFTFNVTGPITANVRLGPNASLQIGAHELGNIYISSLNVAVADGSWVDIWAH"
BASE COUNT         1643 a         2275 c         2595 g         1463 t
ORIGIN      
        1 tctactcgga gtcggcgacc gtgagcccgg acaaccgcca catcgccctc gtggcgaaga
       61 agaacgaccg gttcgccctg atcgtggacg gcaagatgca caaggagtac gaggccatca
      121 gcaaggacac gcctcccagg ttcagccccg actcgaggca cgttgcttac aaggtcaaga
      181 agggcaggaa gtggctggtc gtgctcgacg gcgacgagct gaagccttac gacaacgtgg
      241 gcggcatcac cttcagcccc gactccaggc gcctcgccta cgtcgcgcag aagggcgcag
      301 actatatcgt ggtcctggac ggcgtggagt cgaaggccta cgacggcata ccgcacacga
      361 acaaccccgt gttcagccct gactcgaaga ggctcgcctt cctcgccctc aaggggcaga
      421 agatggtcgt cgtggagggc ggccaggaag ggaaggagta cgagaagatt tcctacccct
      481 tcttcagccc tgactcgaag cacctcgtgt accctgtcat agacaagaag aagatgttca
      541 tcgtgttcga cgggaaggag ttgccccagt acgaaaagat cagcctccag gccttcagcc
      601 ccgactccac gcgcttcgtc tacacggcca ggcagggcga caagtccttc atggtggtcg
      661 acggcaagcc ccagcaaccc ttcacggacc tcgcgctccc cgtgttcagc cccgactcga
      721 agcggctcgc ctacctggcg aaggagggcg agaagttcgt catggtcata gacggcgtca
      781 agacggcccc cttcgacgag gtgctccagt tcggcttcag cgaggactcg tcgaggtact
      841 tcttcacggc ccgcaagggc gacacgtggt ccgtgtacac ggacggcaag cctcaagggg
      901 cctacgacgc ggtgctgctg cccatgttca gccccgattc caggcgcttg gcctacgcgg
      961 ccatgaaggg caagaagtgg accctcgtgg tcgacgggaa gcagacggcg ttctacgatg
     1021 cgatcctcga catccagttc agccctaact cccagcacgt cctgtgcagg gcgatgaagg
     1081 acaacaagtg gaaggccatc ctggacggac gcgagtcgag cacctatgac gtcgtcgttc
     1141 tgcccatgtt cagcccggac tcgaggcatc acgccttcct tgccagcgag aaggggaagt
     1201 ggttcgcggt gatcgacggc aaggaaggaa aggagaggtt cctcatgttc ctcaagggcg
     1261 cccagcagca ggccgtgaac ctgtccatcg acaacgaggg gaggctcagc ctcgtcgcca
     1321 tccaggaaaa caaggacagc acgctgggtc cgaacttcgt gaggtacacg gtggacatgt
     1381 gaggcctctg cccactgtct cgtaccatgc gctgaacggt cgaggtggct ccgtgtacgg
     1441 ttcgtcaagg gtcgtggtgg tgatgcccgc ctaccaggcg gagcacacgc tgcgcatgac
     1501 gtacgacgag gtgatggcgc agggcgtggt ggataccgtc atcgtggtgg atgacaacag
     1561 cacggaccgg acggtggaga tcgccatgac cctgcccgac accatcgtcc accgccacga
     1621 gaggaacagg gggtacggcg ccaaccagaa gtcgtgctac aggctcgcgc tggaagtggg
     1681 cgccgacatc gtcatcatgg ttcaccccga ctaccagtac acgccgaagc tgattcccgc
     1741 catggcgagc ctgatcggca acgggctgta ccactgcgtg ctgggctccc gcatcctggg
     1801 gggctacgcg ctgaagggcg gcatgcccct gtggcgatac gtggcgaacc gcttcctgac
     1861 gcttgccggc aacatcctca tgtcggcgaa gctctcggag taccacacgg gctatcgggc
     1921 gttctcgagg gaactgctcg agcgcgtccc cttcgagcgc aactcggacg acttcgtgtt
     1981 cgacaaccag atgctcgcgc agatcctctg gtacgggtac acgatagccg aggtgagctg
     2041 cccgaccaac tacttcgagg aggcctcgtc catcaacttc gcaaggagcg tcaagtacgg
     2101 cttcggctgc ctgcacaccg cgctggcttt ccggctcgcc aggcacggta tcgtgaggag
     2161 cgagcttttc ccggagccgt cctgacgcag cccggagagc ggtacgcggc cgggcgaggc
     2221 tctccgtgcc tgccggacac ggtcggccct cgtgcctcgt ggcagagaca cgattgacct
     2281 gtctggaaag cacacgcgaa gggggatgta ggacctctcg taaaggagag atggagaaga
     2341 cgccccctgc gtggcagggg gcgtggttcg tggatcgatc ggcccatgcg tcttgatgcg
     2401 gaaacgtggc gtgtcgtctc cgggtgcgtc atgcagatgg ggatgccgtc ggtctgcccg
     2461 gagacgagga gggaggcatc agggtcgacc ccacacctgt tcatgcaggc cgaagaaagc
     2521 catcttgccc gcttgtcctc agcgacccct cacgaggctt cggtagcggt ccgtttccct
     2581 gagtgaagcg aggtcagggt cgctcttcat gaggtcccac cggtcgaacc cgcgcgagac
     2641 ggcttcctcc agcctgtcca tggcctcatc gagcctgccc agcctggcga gcgcgcaaga
     2701 gacgttgtag agtgtctcgg ggtctccggg cctgatttcg agcatgcact cgagctcctt
     2761 gagcgcaccc tcgtaatctc ccgaccgggc cttcctgcgg gctgacgaag cgagggcggc
     2821 gaacccgtct tcgtccaaac cggcgtgccc gcgcacgccc gcttgtgcgc cggaaagcgc
     2881 gccgatagca gcgatggagc gccgggcgtc ctcgttgccg gggtcgagct cgaggactgt
     2941 cgaatactcc tctgtcgcgt cattgggcct gcctgcgagc acgagcgcgt tgccgaggaa
     3001 gaggtgcgcc ctcgtggatg aggggttgag tgtagttgcc cttctaagga cctcgacggc
     3061 attatccgcg cggcctgcct ttgcatatgc ggctcccagc ctctcgaggc tttctcccag
     3121 gcgccgggac tgttccggcc agaagggcga gggttcgatg cccgtgagga tccggctggc
     3181 gacgtccagg tccatgacgg tcgttgcgaa gtcggatgcg gcgagggcgt tgtagaagcc
     3241 cgtccggtac acggtgtcgg agaccgtgtg ccccgtcccc gcaggagcaa ggcgcgaaga
     3301 caggaacact gcggccttcg gcccatagtg cacgagtcgc caatcgggag acgagaggaa
     3361 gaagcgcatg tggggaaagt cgagggagag gacccagaag tcacaccggt atctctggag
     3421 gaaggcgtgg cggtacgcgt cgtcgggccc gtactcgaac gcgttgtatt cggcgtacca
     3481 gccggcatag gggaagtagc gctcgtcgat gaataccttg tgtgtgggcc acaacgacca
     3541 tatcaggtac gatccgcaat tgtaggtgtt gcccatgacg agcccctcga ggttgttcct
     3601 gacgaaggag gcctcgccag cggggtcgct gaagtggagg acgttgagac cggcctgggg
     3661 ggagaagagc gactccacac ccattctcgc cgagaagaag aggaccagga gggatgccgc
     3721 cgcctgcacc gcgatcgtct tgtatgcggc atgccgggct gttccttcgt ctccgggcat
     3781 gacacctccc gttttcttcg caggagacga gaccccagga ccgatgcggc cggatccgcc
     3841 tgtatccaga ggcttgccgg cccggctggg cgtgagcctg gagccggcgt gtccgccccc
     3901 ggcaggggcg ctttccgtga tgcgcctcat gagagagatc gcggtgaaga cgaacacgac
     3961 gccgaagaag taggtggtgc gcatgtagcg aaggaacagg agcgcgaagc cgatgccgat
     4021 ggttacggtc gaccagtcga gggtcctgcg cctggcagcc gcgacgacga ggcctatggt
     4081 cacgacgagg gcggcggcga ggtactcgag gtagtgggtg cccctcacct ctggcgccag
     4141 gatcgagtag tatgcgcgca cggatgcggt gtgtctcgag aaggtctcgg agaagagagt
     4201 ctcgtacagg tatgcaggat agtcccaccc gtacggtgtg aaaaggatcg ccgccgcgct
     4261 caggccgagg gcgacaagcg cggttgggac gagatcggcc ttcagtctcg caggggatct
     4321 gaagagagcg ttcaggactt cccctccagt ggcgcatgcg atgaagaaga ccccgaagat
     4381 gaaccctccg tgcgagttga cccaggcaag gacgagcagg gggatagcaa ggatcgaagc
     4441 ccttgaccac gcccccccgg tcttcgagga aaaccagagg aaagacagga aggcggtaaa
     4501 gaggaaggag aacaactccg ccttcacgag gagccctgcc tgtgacatga ggaggccggt
     4561 gaggcacacg aggatcacca aagggctgag acccggtttt ctcctcaagg cgggggccgc
     4621 catgagcgcg gcaagggcca ggaatgcaca gacgaaaaga tagcgcaagg ccaggagacc
     4681 cggcatgccc aaggcctcgt ggatcttgaa gaagagaacc tggggtatcc atgcgcagta
     4741 gatgggcgag gcgtcagcct gcgtccagct gaaggcggca tggtccggtc tcagcgtggt
     4801 gttctccagc atgtacctgc cgtaggcgag gtgaaaccat atgtcgccgt ctgtcacggg
     4861 ctcggcgtac ctgaggatca ccgccgcgag agaggccgcg gccaggagag ccgccagggc
     4921 gatgcgaagc gcgcgggaga tcatgacaac acccctgcat cagatgtcat ccaagaacca
     4981 ttgattaagt gtataccacg ccatgtcggt gacttcagca cagaaatgca tacatccgtt
     5041 gcaggcccgg agggtcggga cgggaaaacg ccatgtccga ctccgtgagt gagaggcatg
     5101 acaggtccca tggtgtctac gctggcgaca aacaggaggc cgaactgtcc ggggggttat
     5161 gcattgagcc tggaagcggg caggcccgcg tgaggggtgc gttgcgctga tggcctgggt
     5221 gggggtgcct ggcaggtgaa cacgcacagc acgttgaaaa acccgattag ttcggcttct
     5281 cccggcgcac cgggggtggg gtgggcgatg ttgcgggaga gaggcgaagg aatcacgagg
     5341 agaacccttg cacagcgaga cgaccattct ctatgcgaga tgtgaagaag gtggtagtgg
     5401 gctcgatagg gacatgcagt gaccgtggat atccatgctg gatgacgagg cagccctgat
     5461 ggaactgttt tcgcggccca gattgcacgg ctccacgtat ttcactctct aggcctggcc
     5521 gtccatgttc atcggtttct catgaagggc catgcgtagg ggtggatagt gaccgccgag
     5581 agttatgtgg ccatggttgc ctggcgttga ggtgaatcgg aagacatacc accatgaaaa
     5641 agggggccct cccggagggg cccccgaacg ggcggatgtg gtcggtgaag ccgactagtg
     5701 cgcccagatg tcgacccagc tgccgtctgc gacagcgacg ttcaagctcg agatgtagat
     5761 gttgccgagc tcatgcgccc ctatctgaag gcttgcattc ggcccgagcc tgacattcgc
     5821 cgtgatgggg ccggtcacat tgaacgtgaa gttgtcgccg aagaccatgt ggaccacggt
     5881 ggttgggccg tgagctgcat agacccctgt attcacggta cccacgtcga tcctgacgga
     5941 gccgcttatc gagatcggcc cgcccacgac gaggttgtcg agcccgatgt agccggcggt
     6001 gctgttcacg aaccagggaa cgccgccggc gcccacgttg ctgcccgcgc cgtcggtgtc
     6061 accccacgcc aggcggccca tggagaactg gtcgatgatc acgttcatga agaagttcac
     6121 gcccacgccg cccgcggtgt agatgtccac gtaactcgac gggttgatgt acatgatcat
     6181 cgaggcgata ttgacagttc ccaacacctg ggagaggctg gcaccggcag ttccgagggc
     6241 cacgttcatc gtcatggagt tcattcgtat ctcgagggaa ccaaggccga aacgtacgaa
     6301 ggtggcgccg ccgtgggaca agccggagcc gacgtcgatg gtgattggct tcagcatggt
     6361 cgtggtgtag ccgttgaagt tgtctaaggg gttttcgcgg gctgccaccc tgagaccgga
     6421 gatgttgaag ttggtcacac cgacatagcc tgctgtcgtg tcagcggtgc cccagcccca
     6481 ctgggcgaaa ccggtgtcta taccggaagc gtcgccccat gccatcgtac cgatggcgat
     6541 gttcatggtg acgtcggcgt tgatattgac accggcctgg cctgtgacca gggagagctc
     6601 atcgtcggcc accggggtca ttgcccacaa cgacaacggc atgcacagga gcatcatcaa
     6661 gatgagtaaa tatctttgca tagtgtttat ctccttgcct tttgactttt cctcgccgca
     6721 ccctgaattg tttcccgggt ggtaactgat ttctggtttc aataatatcg gagagttggg
     6781 gtgttcacga ctcagggcga tggtctccat tctcgaagaa tggttcgaaa ctcctcgagt
     6841 ccagcggcaa tatcagggtg aaagcaatca aaatgccaga atataataat ggccatgtta
     6901 aaaaaataca aagaatgacg gtaagttaat cagaatctag atacaatgta attagtaggt
     6961 ggcaaacaat tgtaaacatg atgaaaataa tttccacaat aaaacatatt aaaacacgga
     7021 gaaggcatcc tttgttctcc agggtccgga ttgcatggcg gggcggatac ggcctgatat
     7081 tatatagttg tgtctcgagg tgcgggacac tcaacggtgt tcgtttcctt catcaagttc
     7141 cacgggtgca catcaccgtg gggcggcatc atggacatgc gacaggacgg gatcccgccg
     7201 cgtgtgtcga tcaataagcc gcctgggaga atcgggatga tcaccgatcc ctgtcttccc
     7261 attgatcgag agttcctgcc gaacaaggtc caagggttct tctcttcgat gtatcatgca
     7321 aacggtcgtg ggaatggcga tggtcaatgc atccagggtc cttcgcccat gatactgtcg
     7381 aaagggtatg accgtacagg cgcacattca ttgcgcatag cgtcaagagg gggagttggc
     7441 caggtcgatg tcccatcgtg ggacacaggt ggggcaccat ccttcatccg cgcggacggg
     7501 ccatggcaaa tcgaaaaaag tgtcatcgtt tgcaccacga tccttcctct acgcagccgg
     7561 gccacagcgg ccccgaatgc ggcgcccgga ctcatggcgg cgcgcgtgtc aggcgcacag
     7621 cctaaggcca cgcccccttt gatcgggact ggagttgcaa ggacgtgggg atcttgttgc
     7681 agggaatggt gccaaatgga ggttgcaccg cctttggcgg agccattgcc tcacgagaac
     7741 cgaggacgca acgaacacag gggcagggcc ataggcctcc tctcatttgc caaaaaccgt
     7801 ggggagatcg tctcacgtag ttgaagttgc agaaggtttg ctcgggtttg tcggtgtttt
     7861 tcaccgaatt gagagatcct cggtgatgat gtctcacccg tgtgggcttg cgcacggtct
     7921 tgttccggcg cagtgggggt cttgccccgg tcggagaggg cgtctcaggg cctgct
//
LOCUS       DAOSCR010000031         7976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5882_length_7976_cov_3.072592, whole genome shotgun sequence.
ACCESSION   DAOSCR010000031 DAOSCR010000000
VERSION     DAOSCR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5882_length_7976_cov_3.072592"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLT69_01575"
     CDS             complement(<1..425)
                     /locus_tag="PLT69_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOJ13173.1"
                     /translation="MNAVVFASTTAGAVAVAGLVESGFEVKLVITASARGLPHGTDPA
                     VEEVCARLGVACRATDDPGQFGWIERIRRLEPGMIFAAGYPFGLKAQVLALPRHGCFR
                     IHYSLLPAFRGPDPVRTAILSGESTTGVTLHEMETTPYAG"
     gene            complement(442..1878)
                     /locus_tag="PLT69_01580"
     CDS             complement(442..1878)
                     /locus_tag="PLT69_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOJ13174.1"
                     /translation="MGHTERIFLDHASTTAVHPLVLGAMMPFFANHFASPSSPFSAGD
                     IPRRAVEKAREQVASLIGARANEIVFTATGTEANNLAVLGAASSRKGHVLVSALEHPS
                     VIEAAMHLRRSGRDLTILEPSSEGIVDPARLEDAMRPDTILVSVLHANYETGAILDVE
                     AVGRVAKAHGALFHCDCIQTAGRIRVDVDAMGADLVSVSAHKLGGPKGASALYVRSGS
                     KIAPVIFGAARERGLRPGHLNVPCIVGFGAACEHALYTMDERAAILCSLRETLEGEIL
                     ARLPSSRVNAAQARRVPHISSISFEGIPADALCAWLDLEGITASPRGSLFLGSRTERL
                     RLMGIAGDLAFGTVRFSPGPDLTAEDMVRVASSVEQACSRFREFSRMTEEDEACILLC
                     RSTAAVRFALEHLLSEGVVCVATAVPPELEQEGGPRTAVAVPQPLESKALGVLEARGL
                     HGVASRKIKGLCKRRGDKERRFWEEVQG"
     gene            complement(2002..2997)
                     /locus_tag="PLT69_01585"
     CDS             complement(2002..2997)
                     /locus_tag="PLT69_01585"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOJ13175.1"
                     /translation="MGGVLLRSTHIPGRHCASSAISDIVRFHGLDLSEAMCFGIGSGL
                     GAWYLQNVPNAPSRMIHVRSADIEAKFFKRIGCPFSWSRFPGADQALDALRASLHRGL
                     PTLVQTDIYYLGYYQSSTHFPGHDIVVWGCDDQEGVFFVTDTERPDVIRVPSFELARS
                     MFAEGGFFPMEGNSYAPEAIATGIDLGGVIERAIVSNSRLILSEDLEFQGIAGLRLWL
                     REVKTSWPGLEDWRWAARFAYQVIERRGTGGGGFRIMYADFLDEAAPLCPAIAELGLG
                     RMMREASQAWTRLALRLKEASEGDADALGPVGESIEEVIERETAYHRNALRVTGR"
     gene            3113..3565
                     /locus_tag="PLT69_01590"
     CDS             3113..3565
                     /locus_tag="PLT69_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ13176.1"
                     /translation="MTVSDRNFLYTLTVIRRGLFAFLRRRLRENGIEGLEPSSGDILH
                     AMDAKGPLTIGELARCTSKHKSTVSPIVKRLEEMGFVSREKDASDGRRSTLRLSERAD
                     ACRPVLLGLSREMNERLFMGFTEEERRTLFSLMGRVLENARRMACPRG"
     gene            3751..4575
                     /locus_tag="PLT69_01595"
     CDS             3751..4575
                     /locus_tag="PLT69_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ13177.1"
                     /translation="MERIEATIETSMFEDVLCVRMSRVLDGKPLYWVACYLVDGLLVD
                     TGCSHTAHELVSFLEDKEINAVVNTHFHEDHIGANSLLAKRRKVPAYAHPASIPLMEH
                     GFHLYPYQEVVWGIPEPCTALPVPDTIETCRYRFSVIETPGHSEGHICLFEPEKGWCF
                     TGDLFSRENPKFIRPEENVADMVRSLRMIDRLPSERLVLFTSLGRIVEDGRAAVANCI
                     KYLEELSRKVHGLAGSGLDEQGIVERIFGGEHLFASLTDGQYTSLNLVRSILKTAS"
     gene            5090..5767
                     /locus_tag="PLT69_01600"
     CDS             5090..5767
                     /locus_tag="PLT69_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ13178.1"
                     /translation="MKVFRCGLAIGAVAGVLLAMAGCSWDFNNPKADEWDVQIVGDSV
                     FDLSGDIQKVLKTLSGKTYKDRSVSGAKIAAIQSQLNQALQRATLKTVIADGGANDIL
                     QGTMDCDSDPLTQGCIDVLNYVADKMEVMLEAMYNGPPDDCVWLGYYHVKGSQAEKNE
                     AIDYAYDWLYPDVFASTNMSAVLVDPRDEILPEHLKSDNIHPTYAGSQILANLIWDAM
                     VAGNMYR"
     gene            6089..6796
                     /locus_tag="PLT69_01605"
     CDS             6089..6796
                     /locus_tag="PLT69_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13179.1"
                     /translation="MGEGGDSVKSGGAAKRFPLAFAAALVAALCALYLYLDARDDTAR
                     SPATPASSAALGGGQMEPLPEAAPGSRVRAPGALDAAKGDVEAGVLEPTEDIEEIKRR
                     VYRLNIEDVDDLPLLDTVVQVKDKDVKPFWKGEWESVDDFKGSVNGFFLMPREDGTFV
                     FFPDEETTKTYTFFETPKVYTYDPVKREFSWEMDYYGKRISHKARFITDDVLATMLIS
                     GSKVSLELYRRRPPAKE"
     gene            complement(6991..7776)
                     /locus_tag="PLT69_01610"
     CDS             complement(6991..7776)
                     /locus_tag="PLT69_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13180.1"
                     /translation="MGHEGNRTRTSSAVVLLAFFSLVLCGWGSLPGLHKKKPEPKTHF
                     YFGMDEKPVYSRSVVDSPKSRYVFEPALQFDLVMHDFVVKNTTGRTLELNRVSACCGA
                     FVEHHSTQIPPGLEGVIRLVLLTDRKGGQDIAGEVQAQTNDPVHPVWTIEIHCHVRKF
                     ADISDYIITLVGPAGAPIEGSTTIVPTEEYPFTITGLKAKKGRDIDFWYRRTTEHGKK
                     AYVLTVRNKRKEAGVFRDTIFVQTDNKARPEFKIRVQGKVGGS"
BASE COUNT         1396 a         2566 c         2622 g         1392 t
ORIGIN      
        1 cccgcgtacg gggtggtctc catctcgtgg agggtgaccc cggtggtgct ctccccggac
       61 aggatggccg tcctcacggg gtctggcccc ctgaatgcgg gcaggagtga gtagtggatg
      121 cggaagcacc cgtgcctggg cagggcgagg acctgggcct tgagaccgaa ggggtatccc
      181 gcggcgaaga tcatgccggg ctccaagcgc ctgatacgct cgatccagcc gaactggccg
      241 gggtcatcag tcgcgcggca cgccaccccc agcctggcgc agacctcctc caccgcaggg
      301 tccgtgccat gcggcagtcc ccgggcggaa gcggtgatga ccagcttcac ctcgaagccc
      361 gattccacga gcccggccac cgcgaccgcc ccggcggttg tcgaggcgaa gacgacggcg
      421 ttcatgcagg gtcccttccc ttcacccttg cacctcttcc cagaaacgcc tttccttgtc
      481 gccccttctt ttgcacaggc ccttgatctt gcgggatgcc accccgtgca ggccgcgtgc
      541 ctcgagaacg cccagggcct ttgactcgag aggctgcggc accgcaacgg ccgtgcgcgg
      601 cccgccctct tgttcgagct ccgggggcac tgcggtcgca acgcacacga cgccctcgct
      661 taagaggtgt tcgagggcga acctgaccgc ggccgtggac cggcagagga ggatacatgc
      721 ctcgtcttcc tcggtcatcc tggagaactc cctgaaccgc gagcaggcct gctcgaccga
      781 ggatgcgacc cgcaccatgt cctcggcggt gagatcaggg ccggggctga accgcacggt
      841 gccgaaagcc aggtcgcctg caatgcccat gagccggaga cgctccgtgc ggcttcccag
      901 aaagagggaa cccctcggcg aggcggtgat cccttcgaga tcgagccagg cgcagagggc
      961 gtccgccggg ataccctcga acgagatgct cgatatgtgg gggaccctcc gggcttgggc
     1021 ggcgttgacc cgcgacgagg ggagcctcgc gaggatctcg ccctcgagcg tctctctgag
     1081 cgaacacagg atggccgccc tctcgtccat ggtgtacagg gcgtgttcgc atgcagcgcc
     1141 gaaacccacg atgcagggca cgttcaggtg gccgggcctg aggcccctct ccctggcggc
     1201 accgaagatc acgggcgcga tcttcgagcc cgagcgaacg tagagggcgg atgcgccctt
     1261 cggtccgccc agtttgtgcg cggacacgct cacgaggtca gcacccatgg catcgacgtc
     1321 cacccgtatc ctcccggccg tctggatgca gtcgcagtgg aacagggccc catgggcctt
     1381 cgccacccgg cccaccgcct ccacgtccag gatcgcccct gtctcgtaat tcgcgtggag
     1441 cacgctcacg aggatcgtgt cgggcctcat ggcgtcttcg aggcgggctg gatcgacgat
     1501 gccctcgctc gagggttcca gtatggtcag gtcacggccc gatcgtctca ggtgcatcgc
     1561 cgcctcgatc accgatgggt gctcgagtgc gctcaccagg acatgcccct tccgggaaga
     1621 ggccgccccg agcacggcta ggttgttcgc ctccgtgcct gtcgccgtga aaacgatctc
     1681 gttcgcacgc gcgccgatga gcgatgccac ctgctcgcgg gccttctcaa cggccctcct
     1741 ggggatgtcc ccggcagaga agggtgatga cggcgatgcg aagtggttgg cgaagaaggg
     1801 catcatggcc ccgagcacga gcgggtggac cgccgtcgtg gatgcgtgat cgaggaagat
     1861 cctctccgta tgacccatgt cttgtctcct gcacccttgg gtctgtcccc gcgggcatgc
     1921 cgcctgccgg tgtcccctca ttgatcccgc cggagaaacg ggtgcgtctg ccggatgtca
     1981 tagtgcggct gtgccgcggc gtcaacggcc ggttacacgc aaggcgttcc ggtgatacgc
     2041 tgtttctctc tctatgacct cctcgatgga ctcccccacc ggaccgagtg cgtcggcgtc
     2101 cccctcggag gcctccttga ggcggagggc gagccttgtc caagcctgtg acgcctccct
     2161 catcatcctc ccgagcccga gctccgcgat cgccgggcac aggggcgccg cctcgtccag
     2221 gaagtccgcg tacatgatcc tgaagccccc accgcctgtt ccccgcctct cgatgacttg
     2281 gtaggcgaac cgtgcagccc accgccagtc ttcaaggcct ggccacgagg tcttcacctc
     2341 gcgcagccac agcctgagcc ctgcgatgcc ttggaactcc aggtcctccg aaaggatgag
     2401 ccggctgttg gaaacgatcg ccctctcgat gacgccgccg aggtcgatcc ccgtcgctat
     2461 ggcctcgggc gcataggagt tcccctccat ggggaagaac cctccttcgg cgaacatgga
     2521 gcgtgcaagc tcgaaagagg gtacccgtat cacatccggc cgctccgtgt cggtgacgaa
     2581 gaacacgccc tcttggtcgt cgcagcccca taccacgatg tcgtggccgg gaaagtgggt
     2641 gctggattgg tagtagccga ggtagtagat atcggtctga acgagggtgg gcagccccct
     2701 gtgcaggctc gcgcgaagcg cgtcgagcgc ctggtcggcc cccgggaacc tcgaccagga
     2761 gaaggggcac cctatacgct tgaagaattt tgcctcgatg tccgccgagc ggacgtgaat
     2821 catcctggag ggggcgttcg gaacgttctg taggtaccag gcgccgagcc ctgagcctat
     2881 gccgaaacac atggcctccg agaggtcgag cccgtggaac ctcacgatgt cgctgatggc
     2941 gctgctcgcg cagtgtcgcc ccgggatgtg ggtggatcgc aggagcacgc ctcccatggg
     3001 ctcattgtaa ggcaaagcct ctcgagggac aaccccgccg gtcgaaattg ccctcggacg
     3061 acaccggctc gagtgagttg acacggtcaa acaagttttg tacaaaacta atatgaccgt
     3121 ctcggaccgg aactttctct acaccctcac cgtcatccgc aggggtctct tcgccttcct
     3181 gaggagacgc ctcagggaga acgggatcga ggggctcgaa ccctcttcag gagacatcct
     3241 ccacgcaatg gatgcgaagg ggccgttgac gattggagag ctcgcccggt gcacctcgaa
     3301 acacaagtcc acggtgagcc cgattgtgaa acgactcgag gaaatgggtt ttgtgtcgag
     3361 agagaaggac gcaagcgacg gccgccgatc aaccttgagg ctcagcgaga gggcggatgc
     3421 gtgcaggccg gtcctcctgg ggctctcgag ggagatgaac gagcgcctct tcatggggtt
     3481 caccgaagag gagagacgaa ccctgttctc cctgatgggg agggtgctcg aaaacgcacg
     3541 gcgcatggcc tgcccgaggg gctgacggat gcaggaccct ccacggcagt ctcacgcata
     3601 acacgacgtt gttgcttgag cgtagacccc gctttctccg tggacctcgg cgggtttcgc
     3661 gggatgccgg gggcgttgtt gcccgcgcgt ctcgcggtgc ggggctgata cctcgtcacc
     3721 ggcgcatggg gatcgcagga aggaggaccg gtggaaagga tcgaagcgac gatcgagacg
     3781 agcatgttcg aggacgtgct gtgcgtgcgc atgagcaggg tgctggacgg caagcccctg
     3841 tactgggtgg cctgctacct ggtggacggc ctcctcgtcg acaccgggtg cagccacacg
     3901 gcccacgagc tcgtgtcgtt cctcgaggac aaggagatca acgccgtggt gaacacccac
     3961 ttccacgagg atcacatagg ggcgaacagc ctcctcgcga aacggcgcaa ggtccccgcc
     4021 tacgcccacc cggcctccat tcccctcatg gagcacggct tccacctcta cccctaccag
     4081 gaagtggtct ggggcatccc ggagccctgt acggccctgc ccgttccgga caccatcgag
     4141 acctgcaggt acaggttctc cgtcatcgag acccccggcc actcagaggg ccacatctgc
     4201 ctgttcgagc ccgagaaggg ctggtgtttc accggggatc tcttttccag ggagaacccc
     4261 aagttcatca ggccggagga gaacgtcgcg gacatggtga ggtccctgag aatgatcgac
     4321 cggctgccct ccgagcgcct cgtgctgttc acctctcttg ggaggatcgt cgaggatggg
     4381 agggccgccg ttgccaactg catcaagtac ctcgaagaac tttcgaggaa ggtgcatggc
     4441 cttgccggct cgggcctgga cgagcagggg atcgtggagc ggatcttcgg cggcgagcat
     4501 ctcttcgcga gcctcacgga cggccagtac acgagcctga acctcgtcag gtcgatcctg
     4561 aagaccgctt cctgacactg tcctgacggg gaaaccctgc gtccgtactc tgttcggatg
     4621 cgggacggtc ccgaagaccg gttcctgatg ctgtcctcct ccgggagctg cggccgagtg
     4681 cccgggatga ccgccacggg catgagcccg ccctgggtgc gtctttctgg ccgaggggct
     4741 cgtttcgcct cgtgacggga tcgcgggtcc cccgccccga tgaacccctg ggcccaaggc
     4801 caggggtgac acccccgccc ttgtcctgac ctgcccgttg ccgccaggcg gacatgcggc
     4861 aacgtgtcgt tttcacgagg agaacagggg gacaggtatc tcgggtcttg acgatccggc
     4921 ccaccccctt gtcccggcct ttcgtccaat tattatggta ataccgactg acggtgatgg
     4981 accacaaggc ggagggcctg accgcgagcg ggccgttcgc acgaacatgc cgccgatgat
     5041 gtcgcttaag ctctgcggca tcgaacggat accatgaagg agggggatca tgaaggtgtt
     5101 caggtgtgga ttggcaatcg gggccgtcgc cggagtgttg ctggcgatgg cggggtgctc
     5161 gtgggacttc aacaacccga aggccgacga gtgggacgtg cagatcgtgg gcgattccgt
     5221 gttcgacctg tccggggaca tccagaaggt cctcaagacg ctctcgggca agacctacaa
     5281 ggaccgttcc gtcagcggcg ccaagatcgc ggccatccag agccagctga accaggcgct
     5341 ccagagggcc acgctcaaga ccgtcatagc cgacggtggc gcgaacgaca tcctccaggg
     5401 caccatggac tgcgactctg acccgctcac ccagggctgc atcgacgtcc tcaactacgt
     5461 ggcggacaag atggaggtca tgctcgaggc gatgtacaac gggccgcctg acgactgcgt
     5521 ctggctgggc tactaccacg tcaagggcag ccaggcggag aagaacgagg ccatcgacta
     5581 cgcgtacgac tggctctacc ccgacgtgtt cgcctccacg aacatgagcg ccgtgctcgt
     5641 cgacccgagg gacgagatcc tgccggaaca cctcaagtcg gacaacatcc acccgacgta
     5701 tgccgggtcg cagatcctgg cgaacctcat ctgggacgcg atggtggccg gtaacatgta
     5761 ccgctgacgg cctcgctcgg catggccccg cccgagacgg tgcatgcctt gagcggcatg
     5821 tgcggggcat gcgccggtct tcgacgcggg gggcggccgt gggcgaggtc gaggctcgga
     5881 agatcgggcc ttgggcgtcc cctgcgacgc ggggatgcca gagtgcccag gcgaccccgg
     5941 acacgcgcgg gtcgatgagg tggtgtgacc cggctgcagg ggtgctgggg gatctcgagc
     6001 agggcgagtg cgttcaggtg gacgcaggaa ggcgttgggc ggaagaggtg tagaccctca
     6061 aggcagggga ggcagcccgg agcggaccat gggagagggt ggagacagcg tcaagtccgg
     6121 tggagcggcg aagaggttcc ccctggcgtt tgcggcagcc ttggttgcgg ctctgtgtgc
     6181 actctacctc tacctcgacg cgagggacga cacggcccgt tccccggcga cccctgcatc
     6241 ctcggcggcc ctcgggggcg ggcagatgga gcccctgccc gaagcggccc ccggttcgcg
     6301 cgtacgtgcg cctggtgcgc ttgacgcggc taaaggagac gtggaggcag gggtgctcga
     6361 acccacggaa gacatcgagg agatcaagcg ccgcgtctac cgtctcaaca tagaggacgt
     6421 agacgacctg cccctgctcg acacggtggt ccaggtcaag gacaaggacg tcaagccgtt
     6481 ctggaagggg gagtgggaga gcgtcgacga cttcaagggg tctgtcaacg ggttcttcct
     6541 catgcccaga gaagacggga ccttcgtgtt cttcccggac gaggagacca cgaagaccta
     6601 taccttcttc gagacgccca aggtctacac gtatgacccg gtgaaaaggg agttctcctg
     6661 ggagatggac tactacggga agaggatctc gcacaaggcg aggttcatca ccgatgacgt
     6721 gctcgccacg atgctcataa gcggcagcaa ggtgtccctg gagctctaca ggagacggcc
     6781 gcccgcaaag gagtgactcc gcgggaccgt ggcgcccgtg aggagcgggg tttcggggcg
     6841 cctgcccggc gtgagccttc tgtcggaaag acccgcctgc accctgtggc tgccccttgc
     6901 cttgacaggc atgtgccgag aggtctcctt cgagggtccg tcggcgtaca agcgaagggt
     6961 gcgcgccgcc gggcggcgct ccttgctctc tcacgaaccg cccaccttcc cctggacgcg
     7021 gatcttgaac tcgggcctcg ccttgttgtc ggtctgcacg aagatcgtgt ccctgaagac
     7081 accggcttcc tttctcttgt tcctcaccgt gagcacgtag gccttcttgc cgtgctcggt
     7141 agtacgccgg taccagaagt cgatgtcccg gcccttcttc gccttcaggc cggtgatggt
     7201 gaaggggtac tcctcggtcg gcacgatggt cgtcgagccc tcgatcggcg ccccggcggg
     7261 ccccacgagg gtgatgatgt agtcggatat gtccgcgaac tttctgacat ggcaatggat
     7321 ctcgatggtc cacaccgggt ggacagggtc gttcgtttgg gcctggacct cccccgcgat
     7381 atcctggccg cccttgcggt cggtcaacaa gaccagcctg atcacgccct ccaagcccgg
     7441 cgggatctgg gtgctgtggt gctcgacgaa ggccccgcag caggcgctca ccctgttcag
     7501 ctcgagcgtc cttcccgtcg tgttcttgac cacgaagtcg tgcatcacga ggtcgaactg
     7561 gagcgccggc tcgaacacgt agcggctctt cggcgagtcc acgaccgacc tggagtagac
     7621 gggtttctcg tccatcccga agtagaagtg tgtctttggt tcgggcttct tcttgtgcag
     7681 cccgggcagg gagccccacc cgcacagcac gagcgagaag aaggcgagga gaaccaccgc
     7741 cgaagacgtc ctggtgcggt ttccctcatg acccatgggg catcgtcctt tcaccttccc
     7801 tgggggtacg gcatccgccc cccctcgttt catcgccagg tgcatccggg catcgggccg
     7861 tccccgggcg ctcccggtgc gtgggcaccg agacgacctt ccacgaaacc ggtgaggcgg
     7921 gtactacggg cggtactata gcacatgccg gcaggggagg cacgatcgac gttcgt
//
LOCUS       DAOSCR010000032         7941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5910_length_7941_cov_3.621354, whole genome shotgun sequence.
ACCESSION   DAOSCR010000032 DAOSCR010000000
VERSION     DAOSCR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5910_length_7941_cov_3.621354"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLT69_01615"
     CDS             complement(<1..391)
                     /locus_tag="PLT69_01615"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ13181.1"
                     /translation="MRVLVTGVAGFIGFHLAGRLLDEGREVVGIDNLNPYYDPSLKRA
                     RLEMLTPREGFTFRFMDCADRRAMEGLFEGGPFDVVVHLAAQAGVRYSLENPHAYIDA
                     NITGFLHVLEGCRHASVSHLVFASSSSV"
     gene            complement(411..1136)
                     /locus_tag="PLT69_01620"
     CDS             complement(411..1136)
                     /locus_tag="PLT69_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOJ13182.1"
                     /translation="METVVVDLVALIDVDDTLYPKGRGPFALVNHNIDLYVMDMCGMD
                     LDAARSLRRTYICSYGSTLQGLMHEHGCDPVHYLKKVHDVPVEEILKPDPRLREALGR
                     TGCRMAAFTNGSYEYARRILKALGVDDILAEVFSIEYMDFVPKPLSWGYLKAMHLFAA
                     EPGRCLVVDDSEANVRTALELGMHGVKVGGGLAVTTSMDGSPSLLEGASHAPSDEGRA
                     GSRSLSISTIYEIPRVVERLGCN"
     gene            complement(1510..2532)
                     /gene="ruvB"
                     /locus_tag="PLT69_01625"
     CDS             complement(1510..2532)
                     /gene="ruvB"
                     /locus_tag="PLT69_01625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOJ13183.1"
                     /translation="MREFIGQGCLGEDERAYEAAIRPRTLEDYVGQDALKENLRVFIA
                     AARKRGEALDHCLFYGPPGLGKTTLAHIIAHELDVGLRITSGPAIEKSGDLAAILTNL
                     EPKDVLFIDEIHRLSSVVEEILYPAMEEHKLDIVIGQGPSARSIRIDLNPFTLVGATT
                     RAGMITSPLRDRFGIIAHLEFYSEDELTTILRRTSRILGVAAHEDGLREIARRSRGTP
                     RIANRLLRRVRDFAEVLAEGRITPEVADLALSRLDIDKAGLDRMDRRIIETMVQTFSG
                     GPVGIDALAASIGEDKDTIEDVYEPFLIQRGYIKRTPRGRQVCASGYRVVGRTPHPGQ
                     SNLFEE"
     gene            complement(2529..3095)
                     /gene="ruvA"
                     /locus_tag="PLT69_01630"
     CDS             complement(2529..3095)
                     /gene="ruvA"
                     /locus_tag="PLT69_01630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOJ13184.1"
                     /translation="MISLLRGRVQNVSGSQAVIMVGGVGYLVNVPLGTHLPSGQDGEV
                     TLYTMLIVRPDGMDLYGFVDQRQREIFSLLLNVTGIGPKTAMNILSQVDPNRFLDEVV
                     CENLAYLTSLPGIGSKSAQRIVLELKERIAKHHRPRSANVDRNIASDAVAALVTLGYS
                     ETQARRAVGAVSAETVEDLVRKALKELV"
     gene            complement(3092..3586)
                     /gene="ruvC"
                     /locus_tag="PLT69_01635"
     CDS             complement(3092..3586)
                     /gene="ruvC"
                     /locus_tag="PLT69_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOJ13185.1"
                     /translation="MRILGVDPGTRVTGYGILELFSRPHRIVHVCHGTIRPRACDMAG
                     RLREIFDGLSDLVRQYGPSEVAVETTFHAMNAQSALKLGQARGAVVLAAALLDIPVFE
                     YTPTEIKKAACGYGHAGKDQIHAMVCTVLNIPKTAVSSMDASDALAVGLCHASARKLK
                     GVLG"
     gene            complement(3586..4335)
                     /locus_tag="PLT69_01640"
     CDS             complement(3586..4335)
                     /locus_tag="PLT69_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ13186.1"
                     /translation="MSGHNKWSTIKHKKGAADAKRGKLFSKLIKEITVAARMGGGDID
                     GNPRLRAAVNSAKAANMPKENIERAIKKGTGELEGAAYEEITYEGYGPGGVAVLVKVL
                     TDNKNRAVAEIRHLFSKYNGNLGETGCVNWMFDKKGYASIDKSQVDEDALYELALGVG
                     AEDITDTGDTYEILTAPENIEDLRAALDAKGIKYNQLEVQMIPQTNVTLTGKPAEQTL
                     KLLEALEDLDDVQAVSSNFDISDEEMAGLQA"
     gene            complement(4860..5594)
                     /locus_tag="PLT69_01645"
     CDS             complement(4860..5594)
                     /locus_tag="PLT69_01645"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ13187.1"
                     /translation="MILERMIRELPSASGQAPPVKAAGADTFHELMESAARVKTAAGQ
                     ADEAQRQKLAVLLELITIGMSRSVMDALSGSDGDGEPSLAGIPLPPADLGITGLSPGE
                     SKDERSRGMDDMPAKGIDLEGIVDKASATYGVDRRLIVSVIRAESGFDPKATSPKGAM
                     GLMQLMPETARALGVKDAYDPEENVMAGTRFLKDLLERYEGDVEKALAAYNWGPGNLE
                     RNPGRLPRETRTYIARIMGDYLGESG"
     gene            complement(5845..6486)
                     /locus_tag="PLT69_01650"
     CDS             complement(5845..6486)
                     /locus_tag="PLT69_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOJ13188.1"
                     /translation="MAGKLAVYPTRMELLKLRRKLAVAVRGHKLLKDKLEGLTREFRG
                     VAELYRRSRREVDDELPGVLKLFVLAQAASSSRIVEDALEGCRRSLEVEPGQKRIMGV
                     AVPALNVTMGEPEGLYSLVLTPPELDGAIERLRGFLPKLVAMAQLEETVRLLCIEIER
                     TRRRVNALEHTFIPRMTETIRFITGKLDEMERSNTTRLMKIKAARLAQEPEGL"
     gene            complement(6493..7884)
                     /locus_tag="PLT69_01655"
     CDS             complement(6493..7884)
                     /locus_tag="PLT69_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ13189.1"
                     /translation="MKLGIPDAAREYRTVRQVVGPLMLVDGLDGITYGEIAEILLSDG
                     SMRRGRVLEVSGAKALVQVFEGTSGISPSDVKVKFLGKGMELGVSPDMLGRVFDGFGR
                     VKDDGPPIIPERYQNVNGTPMNPFSRDYPNEFIQTGISTIDLLNTLVRGQKLPIFSGS
                     GLPHTRLAAQIARQATVRASGEGFAVVFAAMGITFEESQFFVTDFRRTGAIERTVLFV
                     NLADDPPIERIALPRMALTAAEYLAYEREMNVLVILIDMTNYCEALREISAARREVPG
                     RRGYPGYLYTDLATIYERAGRIKGRKGSITQIPVLTMPEDDKTHPVPDLTGYITEGQI
                     IINRGLHTQGGYPPVDVLPSLSRLKDKGIGAGKTREDHADLMNQLYAAYARGKNAKEL
                     AVILGEGALTDVDLAFVAFADAFEDRFVRQGEDEDRPIEESLRIGWELLTLIPRGELK
                     RVRDEYVDKYLPR"
BASE COUNT         1229 a         2721 c         2462 g         1529 t
ORIGIN      
        1 agaccgacga cgaggacgcg aacaccaggt gggagacgga tgcgtgcctg cagccctcga
       61 gcacatggag gaagcccgtg atgttcgcgt ctatgtaggc gtgagggttc tcgaggctgt
      121 agcgtacccc ggcctgtgcc gcgaggtgca ccacgacgtc aaagggtcca ccctcgaaga
      181 gcccctccat ggcccttcgg tccgcgcaat ccatgaacct gaaggtgaag ccctccctgg
      241 gggtgagcat ctcgagcctc gccctcttga gggaagggtc gtagtacggg ttcaggttgt
      301 cgatcccgac cacctcgcgg ccctcgtcga gcaggcggcc ggcaaggtga aagccgatga
      361 acccggcgac gccggtcacg agcacgcgca tcaagacacc tcctcgccgt tcaattgcac
      421 cctaggcgct caacgacacg aggtatctca taaatggtgg aaatgctcaa ggacctggat
      481 cccgcgcgcc cctcatccga tggtgcgtga gacgcccctt ccaggaggga aggggaaccg
      541 tccatggacg tggtcacggc cagcccgcct cccaccttga ccccgtgcat acccagctcg
      601 agggcggtcc tcacgttcgc ctcgctgtcg tccacgacca ggcaccgccc gggctcggcc
      661 gcgaagaggt gcatggcctt gaggtagccc cacgaaagag gcttggggac gaagtccatg
      721 tactcgatgg agaacacctc cgcgaggatg tcatcgacgc ccagcgcctt caggatgcgc
      781 cgggcatact cgtacgaccc gttcgtgaac gcagccatcc ggcagcccgt ccgcccgagc
      841 gcctctcgaa gcctggggtc gggcttcagg atctcctcca cgggcacgtc gtggaccttc
      901 ttcaggtagt gcaccgggtc gcatccgtgc tcgtgcatga gcccctggag ggtcgagccg
      961 tacgagcaga tgtaggtgcg cctgagcgag cgggcggcat ccaggtccat gccgcacatg
     1021 tccatcacgt acaggtctat gttgtggttg accagggcga acgggcccct ccccttgggg
     1081 tagagggtgt cgtccacatc gatgagggcc accaagtcaa ccacgacggt ctccatcacg
     1141 gggcctcccc gtcggcccct cgttctcgag cgtgacgcag gatcagcacg cacacggccg
     1201 ttgcgtcccg cgagccattc ccctccagcg gggccctcgg tcccgtgccc gggatgtccc
     1261 gcagcgcgtc ttctcccaag attcctggtc cgccgacccc gttgcggccc gcacgacccg
     1321 gcggggagcc atgcagagcg caccctcgcc ctgcacctgc cgcggccgtc gcagggcgtc
     1381 tcgggacctt gagcgcaccc gcctcccgtg cggcgccgcc ggtctctcgt gccggcacat
     1441 cgagtccgcc cctcgcccgc ccacccgctc ccttccggga acacggcggg tttgggtgca
     1501 ggcgagggcc tactcctcga acaggttcga ctggcctggg tgcggggtcc gccccacgac
     1561 cctgtacccg ctcgcgcaca cctgcctgcc gcggggagtc cgcttgatgt agccccgctg
     1621 tatgaggaag ggttcgtaga cgtcctcgat ggtgtccttg tcctcgccga tggaggccgc
     1681 aagggcgtct atgcccacgg gcccgccgga gaacgtctgc accatggtct cgatgatcct
     1741 gcggtccatc ctgtcgaggc ctgccttgtc gatgtcgagc ctcgacaggg ccaggtccgc
     1801 gacctcgggc gttatccggc cctcggccag cacctcggcg aaatccctga ccctcctgag
     1861 caggcggttc gctatgcggg gcgtcccccg cgagcggcgg gcgatctccc tgaggccgtc
     1921 ctcgtgggcg gccaccccga ggatcctcga ggtgcgcctg aggatggtcg tcagctcgtc
     1981 ctcggagtag aactcgaggt gggcgatgat gccgaagcgg tccctcagcg gcgaggtgat
     2041 catgcccgcc cgtgtggtcg cccccacgag cgtgaaggga ttgaggtcga tgcggatgga
     2101 ccgggcagaa ggcccctgtc ctatcacgat gtccagcttg tgctcttcca tggcggggta
     2161 gaggatctcc tccaccacgc tcgagaggcg gtggatctcg tcgatgaaga ggacatcctt
     2221 gggctcgagg ttcgtgagga tggccgccag gtcgcccgat ttctcgatgg cgggcccgct
     2281 cgtgatcctg aggcccacgt cgagctcgtg ggctatgatg tgggcgagcg tcgtctttcc
     2341 gagacccggc ggtccgtaga acaggcagtg gtcgagcgcc tcgcccctct tgcgggcggc
     2401 ggcgatgaag acccgcaggt tttccttgag ggcgtcctgg cccacgtagt cttccagggt
     2461 gcgcggcctg atcgccgcct cgtaggcccg ttcgtcctct cccagacaac cctggccgat
     2521 gaattccctc atacgagctc cttgagcgcc ttgcgcacga ggtcttcgac cgtctcggcg
     2581 ctcaccgccc ccacggccct cctggcctgc gtctcggagt acccgagcgt gaccagggca
     2641 gccacggcgt cggaggctat gttccggtcc acgttggccg acctcggtcg atggtgcttg
     2701 gcgatgcgtt ccttgagttc caggacgatc ctctgtgcgc tcttggaccc gatgccggga
     2761 agcgaggtga ggtacgcgag gttctcgcac accacctcgt cgaggaacct gttgggatcg
     2821 acctgggaga ggatgttcat ggcggtcttg ggcccgatgc ccgtcacgtt caacagcagc
     2881 gagaagatct cgcgctgcct ctggtccaca aacccgtaga ggtccatccc gtcgggtctc
     2941 acgatgagca tggtgtacag ggtcacctcg ccgtcctggc cggagggcaa gtgcgttccc
     3001 aggggcacgt tcacgaggta tcccacgccc cccaccatga tcaccgcctg gctcccagac
     3061 acgttctgta cgcggcccct gagaaggctg atcacccgag gacccccttc agcttcctgg
     3121 ccgatgcgtg gcacaggccc accgccaggg catcggaggc gtccatggag gagaccgcgg
     3181 tcttggggat gttcagcacg gtgcagacca tggcgtgtat ctggtctttc ccggcgtgac
     3241 cgtacccgca ggcggccttc ttgatctcgg tgggggtgta ctcgaacacc gggatgtcca
     3301 ggagagcggc ggcgagcacc accgcacccc gcgcctggcc gagcttcagt gcgctctggg
     3361 cgttcatggc atgaaaggtc gtctccaccg ccacctccga tgggccgtac tgcctcacga
     3421 ggtccgaaag cccgtcgaag atctccctca gcctgccggc catgtcgcat gccctgggcc
     3481 gtatggtccc atgacacacg tgcactatgc ggtggggtct cgaaaacagc tcgaggatac
     3541 cgtagccggt caccctcgta ccaggatcga cgcccaagat ccgcattatg cctggagccc
     3601 ggccatctcc tcgtcgctga tgtcgaagtt cgaggacacc gcctgcacgt cgtccaggtc
     3661 ctccagcgcc tccagcagct tgagcgtctg ttccgcgggc ttcccggtga gcgtgacgtt
     3721 ggtctgcggg atcatctgca cctcgagctg gttgtacttg atccccttcg cgtccagggc
     3781 agccctcagg tcctcgatgt tctcgggcgc cgtgaggatc tcgtaggtgt ctcccgtgtc
     3841 ggtgatgtcc tccgccccca ccccgagcgc gagttcgtag agcgcgtcct cgtcgacctg
     3901 tgacttgtcg atggacgcgt agcccttctt gtcgaacatc cagttcacgc acccggtctc
     3961 gcccaggttg ccgttgtact tcgagaagag gtgccgtatc tccgccacgg cccggttctt
     4021 gttgtccgtc aagaccttga cgagcaccgc aacaccgccg ggcccgtacc cttcgtaggt
     4081 aatctcctcg taggcagccc cttcgagctc gcccgtcccc ttcttgatgg ccctctcgat
     4141 gttctccttg ggcatgttcg cggccttggc cgagttcacc gcagccctga gcctggggtt
     4201 gccgtctata tccccgcccc ccatgcgggc cgccacggta atctccttga tgagcttgga
     4261 gaagagcttg ccgcgttttg cgtccgccgc gcccttcttg tgcttgatgg tggaccattt
     4321 gttgtgaccg gacatcgagg accctccccc ctttccagca tgccttgggt gaaccccgta
     4381 tttagcacag tgatgggtgc aagacaagcg gaccggcttc cgaaaggccg gtccgcatct
     4441 tcccccgtcg aggtgttgcc acgtcagatc agggcggcct cggggcacgg gcgacaccgg
     4501 caccggttcg ggcctgccgt ctcctcgcgc agtctcgcgg atcgttcacc ctcgtccgtg
     4561 ggcgccgagc cctgtccatg gccatccagg cccaacgatg ccggacagag gcaaaccctg
     4621 ccgcttcatc gtctcgatga gagaaggccg ggatggaggg gcccgtcgcg acgagacctc
     4681 accgccatca agggccttcc ccgtgtgccc taggcgccgt ccgacgccat gttcacaccg
     4741 ccttcaccct tgctttttct ctcctcccgt atgggacagg gggaacccca gccggggaca
     4801 gcgataccgg tggtctcagg cggctctttt cagggtgggc ctcgggatca cggccgtggt
     4861 catccgcttt cacccaggta atcacccatg atcctcgcta tgtaggtgcg ggtctcccgg
     4921 gggagcctgc cggggtttct ctccaggttt cccggtcccc agttgtaggc tgcgagcgcc
     4981 ttctcgacat ctccctcata cctctcgagg agatccttga gaaagcgggt gcccgccatg
     5041 acgttctctt cgggatcgta ggcgtccttc acaccgaggg ccctcgccgt ctcgggcatg
     5101 agctgcatca gccccatggc acctttcggg gaggtggcct tcgggtcgaa gccgctctcg
     5161 gcacggatca ccgagacgat gaggcgcctg tcaacgccgt aggtcgcgga ggccttgtcg
     5221 acgataccct cgagatcgat gcccttggcg ggcatgtcat ccatcccgcg ggaccgttcg
     5281 tcttttgact ctccagggga gagaccggtt atcccaaggt cggcaggcgg caaggggatc
     5341 ccagccaggg acggttcgcc atcgccgtca gagccggaca gggcgtccat aacagaccgg
     5401 ctcatcccga tggtgatcag ctccaggagg acggcaagct tctgtctctg tgcctcgtcg
     5461 gcctgccctg ctgcggtctt cacccttgcc gccgattcca tgagctcgtg aaaggtgtcg
     5521 gctccggcgg ctttcacagg gggggcctgg ccgctcgccg acgggagttc cctgatcatt
     5581 cgttccagta tcaaagcggg tgggaccctc atcgccactc ccttgcaaga gtatgccctt
     5641 aagcttacaa aacccgtgcc acatccggcc tcttcacggt ggagagtccc atgaagacct
     5701 tccgtcccgc tctcgacatc gaggggggcg tccgggatcg ccttccgccg aacgttcacc
     5761 acgggcgcgc ccgctgtctt cgcacggggg aggcggccga ggcggggcct cgacggggat
     5821 cctgcgtgca tggccgcgag aggttcaaag cccctcgggt tcctgggcga gccttgcggc
     5881 cttgatcttc atgagcctcg tcgtgttgga gcgctccatc tcgtcgagct tgccggtgat
     5941 gaaccggatg gtttcggtca tgcgcgggat gaaggtgtgt tccagggcgt tcacccgcct
     6001 cctcgtccgc tcgatctcga tgcagaggag gcgcaccgtc tcctccagtt gggccatggc
     6061 cacgagcttg gggaggaagc ccctgaggcg ctcgatcgca ccgtccagct ccgggggcgt
     6121 caggaccagg gagtacaagc cctcgggctc gcccatggtc acgttcaatg cgggtaccgc
     6181 tacccccatg atcctctttt gccctggctc cacctcgaga cttctccggc agccctcgag
     6241 agcatcctcc acgatcctgc tcgaagacgc ggcctgggcg agcacgaaga gcttgagcac
     6301 ccccggcaac tcgtcgtcca cttccctgcg agaccgccta tagagctccg ccacccccct
     6361 gaactcgcgc gtgagcccct cgagcttgtc cttgaggagc ttgtggcccc gcaccgcgac
     6421 ggcgagcttc ctccgcagct tgaggagctc catccgggtg gggtagactg cgagtttccc
     6481 ggccataccc cgtcacctcg gcaggtactt gtccacgtat tcatccctga cgcgcttgag
     6541 ctcgccccgc ggtatgagcg tgagcagctc ccacccgatg cgcaggctct cctcgatggg
     6601 cctgtcctcg tcttcaccct gcctcacgaa acgatcctcg aacgcatcgg cgaaggcgac
     6661 gaaggcgaga tccacgtcag tgagcgcacc ctcgccgagt atcacggcga gctccttggc
     6721 gttctttccc ctggcgtagg cggcatagag ctggttcatg agatccgcgt ggtcctccct
     6781 ggtctttccc gcaccgatcc ccttgtcctt gagcctggac aaagagggca acacgtccac
     6841 cggcgggtag ccgccctggg tatggagccc cctgttgatg atgatctgtc cctcggtgat
     6901 gtacccggtg aggtccggca ccgggtgggt cttgtcgtcc tccggcatgg tgagcaccgg
     6961 tatctgcgtg atggacccct ttctcccctt gatcctgccc gccctctcgt agatggtcgc
     7021 caggtcggtg tagaggtagc cgggatagcc cctccttccc ggcacctccc ggcgggccgc
     7081 cgagatctct ctcagcgcct cgcagtagtt cgtcatgtcg atgaggatga cgagcacgtt
     7141 catctccctc tcgtaggcga ggtactccgc cgcggtgagc gccatgcggg gcaacgcgat
     7201 gcgctcgatc ggcgggtcgt cggccaggtt gacgaagagg acggtgcgtt ctatggcgcc
     7261 ggtacgcctg aagtcggtca cgaagaactg cgactcttcg aaggtgatcc ccatggcggc
     7321 gaagaccacg gcaaacccct cgccgctcgc cctgaccgtc gcctggcgcg cgatctgtgc
     7381 ggcaagacgc gtatggggga gacccgaccc cgagaatatc ggcagcttct gccccctgac
     7441 gagcgtgttg agcaggtcga tggtcgagat ccccgtctgg atgaactcgt tcgggtaatc
     7501 cctcgagaac gggttcatgg gcgtgccgtt cacgttctgg taacgctcgg ggatgatggg
     7561 cgggccgtcg tccttgaccc ggccgaaccc gtcgaacacc ctgccgagca tgtccgggga
     7621 gacaccgagc tccataccct tgccgaggaa cttcaccttc acgtcgctgg gggagatccc
     7681 gctcgttcct tcgaagacct ggacgagcgc cttggcgccg ctcacctcga ggacccgacc
     7741 cctgcgcatg gagccgtccg agaggaggat ttccgctatc tcaccgtagg tgatgccgtc
     7801 gaggccgtcc accagcatga gcgggcccac gacctgcctc acggtcctgt attccctggc
     7861 tgcatcaggt atgcccagtt tcatggccgt tcctctccct tttctctccc ccgatacccc
     7921 tcaccccgcc agtgcgccga c
//
LOCUS       DAOSCR010000033         7893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5955_length_7893_cov_2.985838, whole genome shotgun sequence.
ACCESSION   DAOSCR010000033 DAOSCR010000000
VERSION     DAOSCR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5955_length_7893_cov_2.985838"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PLT69_01660"
     CDS             complement(<1..1531)
                     /locus_tag="PLT69_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13190.1"
                     /translation="MRNTKTFAILFIAAASVLLSTQARADDTELFGGVVNIPPNVLII
                     FDNSGSMDETVEVIHNSTPYDPSTVYSGPYRRDRVYRFRTTYRDWVSLVNIGSNYYVD
                     ESEINCEATRVALNTKGTWSGYINNNAPYRCTPVTTSWTLRTGNYLNYLQTGTVEYRR
                     KIDIAKDTIANIIHTTEGVRFGVMIFNNNEGGRILAPIATRESSTAKEALIAQIRALS
                     PTTWTPLAETLAEAGLYYARKQSWFNSGVDYATAYDPAIQYRCQKNYIIIMTDGESTQ
                     DRNSKLWATTYLNGKVIGDYDQDSAIHDEYHWVSSTGALIAYADYGSDYLDDVAKFLH
                     DEDLLGDIPDQAGMSFNEQGFEKQNIEIYTIGFAIDHKLLSETADKTHGNGKYFTTTG
                     SLNLEDIFVSIIANILERNTQFVSPVVPVNRMNRTYADNAIYLGLFSPDSEHPGLWKG
                     NLKKFGLSDTGEILDKYGKRAIWRCQVLDDRPDSPTYGSLIAIGRAATDPGYTPSPFV
                     KA"
     gene            complement(1571..2035)
                     /locus_tag="PLT69_01665"
     CDS             complement(1571..2035)
                     /locus_tag="PLT69_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HOJ13191.1"
                     /translation="METRDRAPSIERILGNEDGAVLVLVLIVLVAAMVIGVMMMRTSS
                     LESRMAGNERRYILDFANLESAVNLVLVQSTSALESIADSTGASFDYPPGTIPEGTRV
                     RVTLARIGKPPVGSGSDTSLRARYYIVDAEDEEGNQAIEVGAYKLFPQTTDQ"
     gene            complement(2047..2442)
                     /locus_tag="PLT69_01670"
     CDS             complement(2047..2442)
                     /locus_tag="PLT69_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ13192.1"
                     /translation="MVRCKDEKGFTLIELVVAILVFALGIMGILKMHQAAVQANNFSM
                     QLTEAANAADNVKEYLRGLPFTHDSMSVGTHDTTQTVLRNIPYNVSYRVDSVAGSGDA
                     ARTVTITVTWREKGTNHLFELPFVIDRVD"
     gene            complement(2545..3186)
                     /locus_tag="PLT69_01675"
     CDS             complement(2545..3186)
                     /locus_tag="PLT69_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ13193.1"
                     /translation="MRDMRCLSNESGVTLVELMVVLVLSMLLMGAVYMTFQLQHSSAR
                     AQTDVSAVQNDIRVAIDIVALDIMHAGLSTNPSRRIPGIPGGTSGAHTLTLQMDVDQD
                     GALTSAGEIIVYSLDADGNLVRNDPNAGVTHVVANGVTALEFRYRGVTSAGVIQDIDP
                     GAGTLDDAQAGAVRLVNVTITKQADRVDSQTGNPIQRTLSRWIARRNGAIEAL"
     gene            complement(3183..3671)
                     /locus_tag="PLT69_01680"
     CDS             complement(3183..3671)
                     /locus_tag="PLT69_01680"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOJ13194.1"
                     /translation="MKQRLALHRGAIPAFLAGRSGLTLMEVMVVVAIIGILASLAIPN
                     FSGWMAKRRLDSTAREVFTDFQRARSEAITRGRSVSIVFDDGADSYLVRDSAGEVIVP
                     EKGMPDEMDLAVEVTTTGFTFRGFLTTDEVTVTLTSAAAPSGARQKMIVVGRGGTVRI
                     EP"
     gene            complement(3673..5961)
                     /locus_tag="PLT69_01685"
     CDS             complement(3673..5961)
                     /locus_tag="PLT69_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOJ13195.1"
                     /translation="MLRHVGADYPLVTVFAGSAAAVLAYEHQWGCLAFTVSILALVLS
                     RSRLVHAAVGVALGLAACLAAPGWLDVGEGVHRVLGRVSSLDYPGSSPRVTIERLVVD
                     GARVRGSARVLLSGDAPEILEGDVITADIVAMPPKGFGNEGEFDLRRHLLETGVVLDA
                     RLAPGGSARVIGTGRKEGLRNRMIRMLEGFSRPEAELIKAMVLGDRSGITPGIRDSFE
                     GLGVSHLLAISGLHIGIALMIGYASASLLLRMAAIFFPALDTPMIARLAAAAWAVWYT
                     VFAGAGVTVMRACIMAVCVAAGTTFLKRKTDVLEGLALAGVIILVVWPGSLSSASFLL
                     SFSAVFGIASALRASQGCPLWFRSLAVSLAPGVFTLPLVIRLFGFVSLVGFAANLVVV
                     PLFGLFIMPCSAAGLAASLVCEPLGSFLLGLSMDTLGLIVSAGQVLGRAQAVPVPSVP
                     WVCACFAGFIIAFWGRPKSFRTLVLSLLCMVIAAIPLAGSMSRARSPMQFDFISVGQG
                     DSILVTQGRHAMLIDAGPSRPGFDAGRYVVGPHLARRGYVWLDLVVVTHLHPDHAGGV
                     PFILSRFPVGRVWVNSWKDGNPSFMEIQGIARKRHLLLHSARRGDTYRLGSASVRVLA
                     PLPGLEGHSTKVDQNVQSIVLMVTDGRTRGLFMGDADMFGELVQVHLQTDLKADVLKV
                     AHHGGKHSCLDPFLEAVRPDVAVISCGRGNPHGDPSPQALERLEKRNIRVFRTDIHGE
                     VLLRSCPRGLSIKSARNPADTQ"
     gene            complement(6139..7050)
                     /locus_tag="PLT69_01690"
     CDS             complement(6139..7050)
                     /locus_tag="PLT69_01690"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOJ13196.1"
                     /translation="MELTTRLADGLIIANPVMNASGTFAYGEEFSGFFDLSVLGAVVT
                     KGLSMQPKRGNPLPRIVETPCGMINSIGLENVGIHAFTREKLPFLRGYGVPVIVNFFG
                     SNEEEYVQAARALDIEGVAALEMNVSCPNVKRGGIEFGKDPEGLNLLVTRVRQACSKP
                     LIVKLSPMVTDICANAQAAVEAGADALTCINTIPAMAIDEETMRPVLGNVTGGLSGPA
                     IKPVALKIVWEVSRRVSVPVIAAGGIASASDAVQFMLAGASAVQVGSASLRDPFCFPV
                     VIEGIRAYLERKGITAVKDLVGSIREG"
     gene            complement(7054..>7893)
                     /locus_tag="PLT69_01695"
     CDS             complement(7054..>7893)
                     /locus_tag="PLT69_01695"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOJ13197.1"
                     /translation="HVSTWRGYYTDTNEDASIMVKDLRVRPIADAEAAIVENREVAPG
                     TWRLELDCAIPQAEPGQFVMVQVDEGLDPFLRRPLAVLSQRKGRVELLYRVRGRGTAL
                     LAKKNQGEGLRILGPLGRGFTKRASDRTIYVAGGTGLPPLVFLAERLEAGVFIVGAKT
                     ISETALLDRVGAIPGVALVVMTEDGSSGDIGLATDGLRRVMDETPDGEEIAVYACGPM
                     GMLKEAAALARERGARIEVSLEERMGCGFGVCAGCVVETRHGLVRVCREGPVFDAEDL
                     LWG"
BASE COUNT         1385 a         2633 c         2401 g         1474 t
ORIGIN      
        1 gtgctttgac gaacggggac ggcgtgtatc ccgggtcggt ggcggcacgt ccgatggcga
       61 tcagcgatcc gtaggtgggg gagtcgggtc ggtcgtcgag gacctggcat cgccagatcg
      121 cccgcttgcc gtacttgtcc aggatctcac cggtgtcgct caggccgaac ttcttgaggt
      181 tgcccttcca caggcccggg tgttcgctgt cgggcgagaa gagcccgaga tagatggcgt
      241 tgtccgcgta cgtcctgttc atcctgttca cgggcaccac gggcgagacg aactgggtgt
      301 tgcgctcgag gatgttcgct atgatcgaca cgaagatgtc ctcgaggttc aggctgcccg
      361 tggtggtgaa gtacttgccg ttgccgtggg tcttgtccgc agtctcgctc aaaagcttgt
      421 ggtcgatggc aaagccgatc gtgtagatct cgatgttctg cttctcgaac ccctgctcgt
      481 tgaaggacat gccggcctgg tccggtatgt cccccaggag gtcctcgtcg tggaggaact
      541 tggccacgtc gtcgaggtag tcgctcccgt agtcagcgta ggcgatgagg gcgccggtgg
      601 aggacaccca gtgatactcg tcgtggatcg cggagtcttg gtcatagtca cctatgacct
      661 tgccgttgag gtacgtggtc gcccagagct tgctgttgcg gtcctgcgtg gactcgccgt
      721 cggtcatgat gatgatgtag ttcttctggc agcggtactg gatcgccggg tcataggcgg
      781 tcgcgtagtc cacgcccgag ttgaaccacg actgtttccg tgcatagtag aggccggcct
      841 cggcgagggt ctcggccagg ggcgtccacg tcgtcgggct caaggccctg atctgggcga
      901 tcagggcctc cttggccgtc gaactctctc tcgttgcgat cggcgcaagg atgcggccgc
      961 cctcgttgtt gttgaagatc atgacaccga agcggacgcc ctcggtggta tggatgatgt
     1021 tcgcaatggt gtccttggcg atgtcgatct tcctcctgta ctcgaccgtg cccgtctgga
     1081 ggtagttcag gtaattcccc gtcctcaggg tccagctcgt cgtcaccgga gtacaccggt
     1141 acggggcatt gttgttaatg tagcccgacc aggtgccctt cgtgttcaac gccacgcgcg
     1201 tggcctcgca attgatctcg ctctcgtcca cgtagtagtt gctcccgatg ttcaccaggg
     1261 agacccagtc gcggtaggtc gtcctgaacc ggtagaccct gtcccttcga tacggaccgg
     1321 aatacacggt cgacgggtcg tatggcgtac tgttgtggat cacctccacc gtctcgtcca
     1381 tactgccgga attgtcgaag atgatgagca cgttgggcgg tatgttgacg acccccccga
     1441 agagttccgt gtcgtcggct cgagcctgcg tagagagcaa aacgctcgct gccgcgatga
     1501 aaaggatggc gaatgtcttt gtgttcctca tggtctcacc tctgaagaac cgatatcgat
     1561 ccgacagttc tcactggtcg gtggtctgcg gaaagagctt gtacgccccc acctctatgg
     1621 cctggttgcc ttcctcgtcc tcggcgtcga cgatgtaata cctggccctg agcgacgtgt
     1681 cgctccccga gccgaccggc ggcttcccga tcctcgccag ggtgaccctc acgcgcgtcc
     1741 cttccggtat ggtccctggg ggatagtcga aggaggcgcc ggtgctgtcc gcaatcgact
     1801 ccagcgccga cgtactctgt acgagcacga ggttgacggc gctctcgagg ttggcgaaat
     1861 cgagtatgta ccgcctctcg ttgccggcca tcctcgactc gagggacgag gtcctcatca
     1921 tcatgacgcc gatgaccatc gccgcgacga gcacgatgag gaccagcacg agcaccgccc
     1981 catcctcgtt cccgaggatc ctctcgatcg aaggtgccct gtctctcgtc tccatgtgcg
     2041 ccgcccctag tccaccctgt cgatgacgaa cggtagttcg aagaggtggt tcgtcccctt
     2101 ctccctccac gtcacggtga tcgtgacggt ccttgccgcg tcaccggacc ccgcaacgct
     2161 atccacccgg taggacacgt tgtacggtat gttcctcagc accgtctgcg tggtgtcgtg
     2221 cgttcccaca ctcatggaat cgtgcgtgaa cgggagcccc ctgaggtatt ccttcacgtt
     2281 gtccgcggcg ttggccgcct cggtgagctg catgctgaag ttgttcgcct ggacggccgc
     2341 ctggtgcatc ttgaggatcc ccatgatgcc gagggcgaac acgaggatcg ccaccacgag
     2401 ctcgatgagg gtgaagccct tctcgtcctt gcacctcacc acgcccgtct ctcctttgcc
     2461 gcttcccggt gaggacgggt ccttcagcat cgtgtgtgat cctacccttc ttctcacgcc
     2521 cgtgtctccc ccgtcgcccc ctggctacag cgcttcgatg gcgccgtttc tcctggctat
     2581 ccaccgggac agggtcctct ggatcgggtt tccggtctgg ctgtcgaccc tgtccgcctg
     2641 tttcgtgatg gtgacgttca cgagcctgac cgcgcccgcc tgagcatcgt cgagcgtgcc
     2701 agcgcccggg tcgatgtcct ggatcacccc tgccgacgtg acgccgcggt aacggaactc
     2761 gagggccgtc acgccgttcg ccacgacatg cgtgacccct gcgttcgggt cgttgcggac
     2821 gaggttgccg tcggcgtcca gggagtagac gatgatctcg cctgcgctcg tgagcgctcc
     2881 gtcttggtcc acgtccatct ggagggtcag ggtgtgggcc ccgctcgttc cgcccggtat
     2941 ccccgggatc ctcctggacg ggttcgtgct gaggccggca tgcatgatgt cgagggccac
     3001 gatgtcgatg gcgacgcgga tgtcgttctg aaccgccgag acatcggtct gtgcccgggc
     3061 ggagctgtgc tggagctgga aggtcatgta gacggcgccc atgaggagca tggagagaac
     3121 gagcacgacc atgagctcca cgagcgtcac gccgctttcg ttcgacaggc aacgcatatc
     3181 cctcatggtt ctatcctcac cgtaccgcct ctgccgacca cgatcatctt ctgcctggca
     3241 cccgacggtg cagcggcgct cgtcagggtg accgtcacct cgtctgtggt gaggaaaccc
     3301 ctgaacgtga agcccgtcgt ggtcacctcg acggcaagat ccatctcgtc gggcatgccc
     3361 ttctccggaa cgatgacctc gcctgcggag tccctgacga ggtacgagtc cgcgccgtcg
     3421 tcgaacacga tcgagaccga tctccccctc gttatggcct cgctcctcgc cctctggaaa
     3481 tcggtgaaca cctcgcgcgc cgtcgaatcc agccgcctct tcgccatcca ccccgagaag
     3541 ttggggatgg cgaggctcgc gaggatgcct atgatggcga ccaccaccat gacctccatg
     3601 agcgtcagcc ccgaccggcc ggccaagaaa gccgggatcg cgcctctatg cagggctagc
     3661 ctctgtttca tcctattggg tatcggcagg attcctggcc gactttatcg ataaaccacg
     3721 aggacaagat cgtaacagga cctcgccgtg gatgtccgtg cgaaataccc ttatgttcct
     3781 cttttccaat ctctcgagcg cctggggtga aggatctccg tgtggattgc ccctgccgca
     3841 ggagatcacc gccacatccg gcctgacggc ctcgagaaag gggtccaggc aggagtgctt
     3901 cccgccgtgg tgagccacct tgagcacgtc agccttgagg tctgtctgaa ggtgcacctg
     3961 cacgagctcc ccgaacatgt ccgcatcccc catgaagaga cccctcgttc tcccgtccgt
     4021 gaccatgagg acgatcgact gcacgttctg gtccaccttt gtcgagtgtc cctcgagacc
     4081 gggaaggggt gcaaggaccc tcacggaagc ggaacccagg cgatatgtat cacctcgccg
     4141 ggccgagtgc aacaacaggt gtctcttccg tgcaatccct tgtatctcca taaacgaggg
     4201 gttgccgtcc ttccatgagt tcacccacac tctgccgacc gggaaacggc tcaggatgaa
     4261 cgggacgccg cccgcatggt cggggtgcag gtgtgtcacc acgacgaggt cgagccagac
     4321 gtaccccctc ctcgccaggt gcgggcccac cacgtacctc cccgcatcga accccggccg
     4381 ggaaggtcca gcgtcgatga gcatggcatg ccgtccctgc gtcacgagga tgctgtcgcc
     4441 ctgccccacg ctgatgaaat cgaactgcat ggggcttctg gccctcgaca tgctcccggc
     4501 caggggtatg gcggcaatca ccatgcacag caaggagagc acgagggtcc tgaaagactt
     4561 tggcctgccc cagaaggcga tgatgaaacc ggcgaaacag gcgcacaccc aaggcacgct
     4621 cgggaccggc accgcctggg cccttccgag gacctgtccg gccgagacga tgagcccgag
     4681 ggtatccatg gacaacccca ggaggaacga ccccaggggc tcgcaaacga gggaggccgc
     4741 gagcccggct gctgaacacg gcatgatgaa gagaccgaac aggggcacga cgaccaggtt
     4801 cgcggcgaaa cccacaaggg acacgaaccc gaaaaggcgt atgacgaggg gcagggtgaa
     4861 gaccccgggc gcgaggctca cggcaagcga cctgaaccac aaggggcagc cctggctcgc
     4921 acgcaatgcc gaggcgatcc cgaagacggc ggaaaaggag aggagaaagc tcgccgacga
     4981 gagcgagccc ggccacacga ccaggatgat cacgccggcc aacgcgagcc cctcgaggac
     5041 gtccgtcttc cttttgagaa aggtcgtccc tgccgcgacg cacacggcca tgatacacgc
     5101 gcgcatgacc gtcacccctg cgcctgcgaa gaccgtatac cagacagccc atgccgctgc
     5161 cgccagacgg gcgatcatgg gggtgtcgag cgccgggaaa aagatcgcgg ccatcctcag
     5221 cagcaaggac gccgaggcgt aaccgatcat gagcgctatg ccgatgtgca ggccggatat
     5281 ggccaggaga tgggagacgc cgagaccctc gaacgagtct ctgatccccg gtgtgatgcc
     5341 tgaacggtcc ccgaggacca tcgccttgat caactcggct tccgggcgcg agaagccttc
     5401 cagcatacgt atcatccggt tcctgagccc ctcttttcgg ccggtcccta tcacccgggc
     5461 cgaccctccc ggggccaggc gggcatcgag caccacgccc gtctccagga ggtgcctccg
     5521 gaggtcgaac tcgccctcgt tgccgaaccc cttgggcggc atcgcgacga tgtccgcagt
     5581 gatgacgtcg ccttccagga tctcgggggc atccccgctc aagaggaccc tggccgaccc
     5641 cctgacacgt gcgccgtcca ccacgaggcg ctcgatcgtc accctggggc tgctgccagg
     5701 gtaatcgaga gacgagaccc tgcccaagac ccggtgcaca ccctctccca catccagcca
     5761 tccgggtgcg gcaagacagg cggcgagccc aagtgcgacc ccgacagcgg cgtgcaccag
     5821 tcgcgaacgg ctcaggacca aggcgaggat cgagaccgtg aacgcgaggc agccccactg
     5881 gtgttcgtac gcgaggacgg ccgccgcact ccccgcaaag accgtcacga gggggtaatc
     5941 ggcgccgaca tgacgcaaca tggcatcttc cctccctcca ggccggaatt caagtccgcc
     6001 cggccgcaca ggagggcttt ctcacaagca ctgaaagaga ggcgggtttt gacctccggc
     6061 ctcaaacgtt catgacaatg gcagaaacac aaaaacgagc cggtgttgac agcccgcggg
     6121 ccgtccccgg acgttcgatc agccttccct gatgctaccc acgaggtctt tgaccgccgt
     6181 gatgcctttc ctctccaggt aggcgcggat cccttctatg accacgggga aacagaaggg
     6241 gtccctgagc gaggcgctgc caacctggac ggcgctcgcg cccgcgagca tgaactgcac
     6301 ggcatcggat gcgctcgcga tccctccagc cgcgatgacc ggtaccgaaa ccctgcgcga
     6361 gacctcccag acgatcttga gcgccacggg cttgatggcc ggtcccgaga gcccccccgt
     6421 cacgtttccc aggaccggtc tcatggtctc ctcgtctatg gccatggcag ggatcgtgtt
     6481 gatgcaggtc agggcgtccg cgcccgcctc gacggccgcc tgtgcgtttg cgcagatgtc
     6541 cgtcaccatg ggcgagagct tcacgatgag cggcttggag caggcctgcc tgaccctcgt
     6601 caccagcagg ttcaggccct ccgggtcttt gccgaactcg atcccccccc ttttcacgtt
     6661 ggggcaggac acgttcatct cgagtgcggc gacgccctcg atgtcgaggg ccctggcagc
     6721 ctgcacgtat tcctcttcgt tggaaccgaa gaagttgacg atcacgggga cgccgtatcc
     6781 cctgagaaac ggcagctttt ccctggtgaa cgcgtggatg ccgacgttct cgagccctat
     6841 ggagttgatc atgccgcagg gcgtctcgac gatcctgggc agggggttgc ctctcttcgg
     6901 ctgcatggac aggcccttgg tcacgaccgc tcccaggacg gacaggtcga agaagcccga
     6961 gaactcctca ccgtaggcga aggttcccga ggcgttcatg acaggattcg cgatgatgag
     7021 cccgtcggcc agtcgggtcg tcagttccaa cgctcacccc cacaagaggt cctcggcatc
     7081 gaacaccggc ccctccctgc acaccctcac gagaccgtgc ctggtttcca ccacgcaccc
     7141 ggcgcacacg ccgaacccgc agcccatccg ctcctcgagg gagacctcga tgcgggcgcc
     7201 cctctccctc gcaagagccg ccgcctcctt gagcatcccc atgggcccgc acgcgtacac
     7261 ggcgatctcc tcgccgtcag gcgtctcgtc catgaccctc ctcaggccgt cggtggccag
     7321 gccgatgtcc cccgaggagc cgtcctcggt catgaccacg agggcgacgc cggggatcgc
     7381 ccccaccctg tccaacagcg cggtctcgga gatcgtcttg gccccgacga tgaagacgcc
     7441 ggcttcgagt ctctcggcga ggaacacgag ggggggcagc ccggtccctc cggccacata
     7501 gatcgtccgg tcgctcgccc tcttggtgaa cccccggccg agcggcccca ggatcctcag
     7561 gccctcgccc tggttcttct tcgcgagcaa agccgtgccc cggccccgaa cccggtagag
     7621 gagctcgacc ctgccttttc tctgcgaaag caccgcaagg ggtctcctca ggaagggatc
     7681 gagcccttca tccacctgga ccatcacgaa ctggcccggc tccgcctgag gaatggcgca
     7741 gtcgagctcg aggcgccatg tgccgggcgc gacctcccgg ttctcgacga tggcggcctc
     7801 ggcatccgcg atgggtctca cccgcaaatc cttgaccatg atcgacgcgt cctcgttcgt
     7861 atccgtgtaa tatcccctcc acgtgctcac gtg
//
LOCUS       DAOSCR010000034         7849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_5990_length_7849_cov_3.183474, whole genome shotgun sequence.
ACCESSION   DAOSCR010000034 DAOSCR010000000
VERSION     DAOSCR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5990_length_7849_cov_3.183474"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1179
                     /locus_tag="PLT69_01700"
     CDS             97..1179
                     /locus_tag="PLT69_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOJ13198.1"
                     /translation="MKIGLIGMAKSGKTTVFNALTGLWAATDQYESARMEPNVAFVRV
                     SDSRVDRLAELYRPRKVTHALVEFVDFCGFSGRRPERKSLFSASDLALARGVHALALV
                     ARNFHETYLDSTLGPPDAASDVENVKTELALNDLVLAETRLERIDHQLKRGAKTPSLA
                     IERSALEKVVAFLEGEARQNMPELTGEEEKSIRGMQFLSLKPMLVILNSDETTFAADP
                     GLVDALGRANRVVEFAGKFEMELSRLDPKDAAALMEDMGIASSARERLTLAAYGLLGY
                     ISFFTVGSDEVRAWTIRKGSTAQEAASSIHTDLSRGFIRAECFSYDDLTAHGSEKALR
                     EKGLIRLEGKNYVVNDGDILSIRSGV"
     gene            1229..2059
                     /locus_tag="PLT69_01705"
     CDS             1229..2059
                     /locus_tag="PLT69_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13199.1"
                     /translation="MSKTRQLLLTLSRVAFAVIVLAVLSCHSENGGQGTVSTEPLLLD
                     LERLYSDVEAITVEVAYETGPEPYTDDGYDRPFWWEILKTNLEALAGPTVNVTVPWEL
                     SRMTGLGPLYQDGWTTSEIVGVAQTLWDATSPPEEQVIHVLFLNGYYEDAEGIKDRML
                     GVSFGGTSLIAVFKDVVESSSGSGLVKRYVEQATLVHEAGHLLGLVNNGVVPCTAHED
                     PEHPRHCANPVCVMFWLNEGPQDLADFVALLSGSGDLVLFCQDCLDDVSSYRSSLSSP
                     "
     gene            complement(2340..2816)
                     /locus_tag="PLT69_01710"
     CDS             complement(2340..2816)
                     /locus_tag="PLT69_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HOJ13200.1"
                     /translation="MGRQVSTSWMVSFLKSFERIVVTALILLMTIVVLLATVDVARIV
                     AYAIADPPLGLITTDSLLDIFGAFLLVLVGIELLYTLRAYWEEDEIHVEVVFAMALIA
                     IARKVIILDVKHLDSLVLLGIAALVVALSAGYYLVARHVKDVCAEKRKIRLTSEGG"
     gene            3134..3448
                     /locus_tag="PLT69_01715"
     CDS             3134..3448
                     /locus_tag="PLT69_01715"
                     /inference="COORDINATES: protein motif:HMM:NF021563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HOJ13201.1"
                     /translation="MDTQRERNLREKLTAEVAVSTWKGLIPDLLSKALFMVSPGVDLV
                     EVGVQVALDNTSQVKAWIESGDIARPTEEQIRVWEASGSLFRFIIVSPFVFFQEFQVP
                     SC"
     gene            complement(3594..4658)
                     /gene="dctP"
                     /locus_tag="PLT69_01720"
     CDS             complement(3594..4658)
                     /gene="dctP"
                     /locus_tag="PLT69_01720"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOJ13202.1"
                     /translation="MEKRTILAILVAAWAAFCMQAARVEAAQAGPEVVIKVASLAPRG
                     SNIATLFEEISQEVYEKTGGAVRFKTYWGGVQGDEQDVLRKVRLGQLHGGAFMGPTLG
                     LIAPEVRVTDLPYIFRNYDEADYVRSKLRPEMERLIEAKGFKVLGWLNLGFVYTFSKV
                     PLDSLETARRQKWWALEGDPVGQAFYRELGITPVSLSISDVATSLSSNMVDCAPATPF
                     GAVALQWYTRFRYMSEYPSSSVYGAMLVRKDVWERVPLQAREVILETASRQYEKIKLT
                     VRAEDDRALELLRKSGIRVMKADFKNKDAAFVFEAAKRARENLVGRLYSRELLDRMLG
                     LVEEYRRLHPEDRRVIRADQ"
     gene            5556..7034
                     /locus_tag="PLT69_01725"
     CDS             5556..7034
                     /locus_tag="PLT69_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOJ13203.1"
                     /translation="MDYKGKAMTTLRSQVFARLGLPILFSAAVLAGCVSVGPDYHEPD
                     TPVPAGWHAVSGKGLAPDPPDAAFLSRWWEGFGDRELTALVHEALQGNLDLAIARARI
                     REAKARRDISMAGFLPFLDASASVTKSDGGDGPSRSLYDASLDARWELDLFGGTRRSV
                     EAAQASYESSEEAFNAALVSLCAEIGINYLNLCTYRERLRVARLNAANQEETYRLARN
                     RYEAGLADALDLEQASSALESTRAGIPVLEDAVEACLNRLCVLTGAAPGALRERLSKT
                     PGLPPLPSTVALGVPADILRRRPDIRRAERELAAQSARVGIAASDLYPKLTLNGTIQL
                     SAERSSELFSSGSRTIGWGPALSVPVFHWGTLKKGVEVQDALLEQAGLSYRATVLSAL
                     EEVENAVSSYAKEAARLENLEKAQVSAQKAFEIAKASYASGNTDFSRLLEAERTLLAA
                     QDSLAQSKGSLAVSLVRLYKVLGGGWPYRPAGMEGARGAQGS"
     gene            7168..>7849
                     /locus_tag="PLT69_01730"
     CDS             7168..>7849
                     /locus_tag="PLT69_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ13204.1"
                     /translation="MDETGILKDKDGETRGSAKARLARVHGRTVKALAVVAVFAAAAI
                     AFFARSEGPGQYVYKTQKAARGDLVVTVSATGTLQPLNEVEVGSELSGIIRTVEADYN
                     DRVRKGQVLARLDTEKLEAKVLQSRATLQTARAKLMEAEATLAESESTLKRLRRLFEL
                     TKGESPSALDLESAQAAAEKARASLASARAQVSQAEANLRADEADLSKALIRSPIDGV
                     VLEFTEDAV"
BASE COUNT         1321 a         2721 c         2598 g         1209 t
ORIGIN      
        1 cccttaagcc cgtcccgtcc accccaaccg gcggttgcgc ctcggtgtcg acggtggtaa
       61 gagagcgatc ctcggagaca ggagggacaa ggctccatga agatcggcct catcggcatg
      121 gcgaaatcgg ggaagaccac cgtcttcaac gccctcaccg ggctgtgggc ggccacggac
      181 cagtacgagt cggcccggat ggaacccaac gtggcattcg tccgggtgtc cgacagtcgt
      241 gtcgacaggc tggccgagct ctaccggccg cgcaaggtca cgcacgccct cgtcgagttc
      301 gtcgacttct gcggcttttc tggcaggcgg cccgagagga agtcgctgtt ttcggcgagc
      361 gacctggccc tcgccagggg cgtgcacgcc ctcgcgctcg tcgcgaggaa cttccacgag
      421 acgtacctcg actcgaccct cggcccgccg gatgccgcat cggacgtaga gaacgtgaag
      481 accgagctcg ccctcaacga cctcgtcctc gcggagacga ggctcgagcg catcgatcac
      541 cagctgaagc gcggcgcgaa gacgccctcg ctcgccatcg agcgctcggc cctcgaaaag
      601 gtcgtcgcct tcctcgaggg cgaggcccgg caaaacatgc cggaactcac gggcgaagag
      661 gagaaatcca tccgcgggat gcagttcctc tccctgaagc ccatgctcgt gatcctcaac
      721 tccgacgaga cgaccttcgc ggcggacccg gggctcgtgg acgccctcgg ccgcgcgaac
      781 cgggtggtgg agttcgcggg caaattcgag atggagctct ccaggctcga cccgaaagac
      841 gctgcagcgc tcatggagga catggggata gcctcgtccg ccagggagcg cctgacactc
      901 gccgcatacg gcctgctcgg gtacatctcg ttcttcaccg tgggctcgga cgaggtcagg
      961 gcatggacga ttcggaaggg cagcaccgcc caggaggctg cctcctcgat ccacacggac
     1021 ctctccaggg ggttcatcag ggcggagtgc ttctcgtacg acgacctgac ggcgcacggc
     1081 tcggagaagg ccctgaggga gaagggcctc atccggctcg agggcaagaa ctacgtggtg
     1141 aatgacggcg acatcctgtc catccggtcc ggcgtgtgat cgaagaggcc gttgccacca
     1201 ggcgcagcgg cgtatatcat ggagggagat gtccaagacg agacagttgc tcctgaccct
     1261 gtcgagggtc gccttcgccg tcattgtcct tgcggtcctc tcgtgccact cggaaaacgg
     1321 cgggcagggg accgtatcca cggagccgct cctgctggat ctcgagcgtc tctactcgga
     1381 cgtggaggcg atcaccgtcg aggtcgccta cgagacgggc cccgagccgt acacggacga
     1441 cggttacgac cggcctttct ggtgggagat cctgaagacc aacctggagg ccctggcagg
     1501 cccgaccgtg aatgtcaccg tcccgtggga actctcccgc atgacggggc tgggccccct
     1561 ctaccaggac ggctggacca cgagcgagat cgtgggcgtc gcccaaaccc tctgggacgc
     1621 cacctcgccc ccggaggaac aggtcatcca cgtgctgttc ctcaacggct actacgagga
     1681 cgccgagggc ataaaggacc gcatgctcgg ggtctccttc ggcggcacga gcctgatcgc
     1741 ggtcttcaag gacgtggttg agagctcttc cggatcgggc ctggtcaaga ggtacgtgga
     1801 acaggcgacc ctcgtgcacg aggcggggca cctcttgggg ctcgtgaaca acggcgtcgt
     1861 cccgtgcacc gcccacgagg acccggagca ccccaggcac tgcgcgaacc cggtctgcgt
     1921 catgttctgg ctcaacgagg ggccgcaaga cctggcagac ttcgtggcgc tcttgagcgg
     1981 cagcggggac ctcgtgctct tctgccaaga ctgcctcgac gacgtctcct cgtaccgatc
     2041 gagcctttcg agtccctgac gttcctgcaa ccggggcctc gggcgccggt tgcgccctca
     2101 cggcacttcc cccggtgcag cgcggcatcc tcgcaccacc ggccggaagg gtgaggcctt
     2161 cgacgggcgc ctgagaaacg cggcgcttga ggcccgcgcc cgaacccggg acgcctcgcc
     2221 accagtgccc tcgagagatg gagcccggtc gggacgcctc aaaggctctc gggccgaggg
     2281 cctggacggc ccccccttgt tccgggccgg ctcggccgtc ccccctcgga caaaggggtt
     2341 catcccccct cggatgtaag cctgatcttc ctcttctcgg cgcagacgtc cttgacgtgc
     2401 cttgccacca ggtagtagcc ggccgacagg gcgacgacga gggcggcgat gcccaggagc
     2461 acgaggctgt ccaggtgctt gacgtccagg atgatgacct tcctggcgat cgcgatgagg
     2521 gccatggcga agacgacctc gacgtggatc tcgtcctcct cccagtacgc gcgcaacgtg
     2581 tagaggagct cgatgcccac gagcacgagc aggaaggcgc cgaagatgtc gagcaggctg
     2641 tcggtggtga tgaggccgag cggcgggtcc gcgatggcgt acgcgacgat ccgtgccacg
     2701 tccaccgtgg cgagaaggac cacgatggtc atgaggagaa tgagcgcggt caccacgatg
     2761 cgctcgaagg acttgaggaa ggagaccatc cacgaggtag agacctgtct tcccatatcc
     2821 gtccttatac cacacacccg cgggcctgca agcgtgtcgc gaaatgtagg gcgacccggg
     2881 ggatgcacct cggccgacac ccctgccttc atccacccac cgcctgggct caccccgcgc
     2941 ggacgccttt cagcggcctg tcaggccgac tctcacgccg gacaaacccc tgccacagac
     3001 gggagccctc ctggggccgc cggcgtttcc cgccctcatc cgctcaggct tcgagccgtg
     3061 cccgccatat tcttcgtgca tcgccgaaag ggcgggtgta tggtggaaag acacaccaga
     3121 gaacgaggag cgcatggaca ctcaacgcga acgaaacctg agggaaaagc tcacggccga
     3181 ggttgcggtc tcgacctgga aggggctcat ccccgacctc ctctccaagg ccctcttcat
     3241 ggtctcaccc ggcgtggacc tggtggaggt gggcgtacag gtggccctcg acaacacctc
     3301 gcaggtgaag gcatggatcg agtcgggaga catcgcccgc cccacggaag aacagatcag
     3361 ggtctgggag gcctcgggct ccctcttcag gttcatcatt gtctcgccgt tcgtgttctt
     3421 ccaggaattc caggtgccgt cctgctgagg acgggccacg cgggaaaaca ggacgacccg
     3481 gccccgggcc tctgccccgg gggagtcgcg tttcaggccg ggcacgaggg tgttcacggc
     3541 ctgggtgaag cgcccgtcgt gggaagcccc gggacgccgt cgacggccca ccgtcactgg
     3601 tccgcccgga tcaccctcct gtcctcgggg tggagcctcc ggtactcttc gaccaggccg
     3661 agcatcctgt ccaggagctc acgggagtag agcctgccca cgaggttctc cctcgccctc
     3721 ttcgccgcct cgaacacgaa cgccgcgtcc ttgttcttga agtcggcctt catgaccctg
     3781 atccccgact tcctgagcag ctcgagggcg cggtcgtcct cggccctcac cgtgagcttg
     3841 atcttctcgt actgcctgct cgcggtctcc aggatcacct cgcgcgcctg gaggggcacc
     3901 ctctcccaca cgtccttcct cacgagcatg gctccgtaca cgctcgagga ggggtactcg
     3961 gacatgtacc tgaaccgcgt gtaccactgg agcgccacgg cgccgaaggg tgtggccggg
     4021 gcgcagtcca ccatgttgga ggacagcgag gtcgccacgt ccgagatgga cagggagacc
     4081 ggcgtgatgc ccagttcgcg gtagaacgcc tgccccaccg ggtctccctc gagggcccac
     4141 cacttctgcc tgcgcgcggt ctccagcgag tcgagcggga ccttggagaa ggtgtacacg
     4201 aagcccaggt tgagccagcc gagcaccttg aaccccttcg cctcgatgag ccgttccatc
     4261 tcgggcctga gcttcgaccg cacgtagtcg gcctcgtcgt aattgcggaa gatgtagggc
     4321 aggtccgtca ccctgacctc gggcgcgatc aaccccaggg tgggccccat gaacgcgccg
     4381 ccgtggagct ggccgagcct gaccttcctc aggacgtcct gttcgtcgcc ctgcacgccc
     4441 ccccagtagg tcttgaacct caccgcgccg ccggtcttct cgtatacctc ctgggatatc
     4501 tcctcgaaga gggtcgcgat gttcgacccc ctgggggcca ggctcgccac cttgatcacg
     4561 acctccgggc ccgcctgcgc ggcctctacc cttgccgcct gcatgcagaa tgcggcccat
     4621 gccgcgacca ggatcgcaag gatggtcctt ttctccatcg catcctccct gccggagtcg
     4681 ccccttcggc tcctgcgaag ccccgcaccg ccggggccgg catccccagg gcgcctggca
     4741 gcctcccggc cggtgtagct ctgcgggccg ggcgcctgcc gcccgagacc ggctttcacg
     4801 gcattctcga gggcactcgg cctgcagatc acccgggcct ccccttgccg gtcgccgggc
     4861 cgaggccgca cgtgcgacag cgggaatgag gccgggctcg aaccactaac cacgatgcgc
     4921 ccagctcgtt gcccggccgc aaccgaaacc tgacatcgaa gaccgacatc agggggttct
     4981 ccccaaagct gccgccaccc gtgagaggct cgcggtttgc cctgtccgtg aggggcgcac
     5041 cctgtatcac atcatatacc cctggtcccg cgcgaagtca cgcgcctgca ccgtaaagga
     5101 acgtgcaggc cctgcatgca ccaggagacc atctcggcag gcacctggaa cgggcgttcc
     5161 cggcgcaggg gcggcccagg gtgacacgcc cgcgccccgg ggagacgcag catcacaccc
     5221 gacggcgggc tttgggctcg gacaggcttc tgcaggggct ttcacgaggg ggaagcgatg
     5281 cccacggcgc cgcaccatgc gcatgggccg cacccggctg ccgctgacct gcaagaaggc
     5341 ctccagcgtg ccggcgggca cccacccgca cagaggtcca acgcctgtcc tcgcgcgagt
     5401 gggtcgagag agcgggatgc cgcatcggct ccggtgaagc ctcctgctgc ccctgggccg
     5461 tcctggccgg gggtcgtgtt tccttcgagg cgtcacgggc ccctcttcgg ataaaaccct
     5521 gccccttcac cgagcgtttg acacggccgt ggtcggtgga ctacaaaggg aaggccatga
     5581 cgacgctgcg ttcgcaggtc tttgcccggc tcggcctgcc gatcctcttt tccgccgcgg
     5641 tcctggccgg gtgtgtgtcc gtaggccctg actaccatga gcccgacacc cccgtgccgg
     5701 ccgggtggca cgccgtctcc gggaagggcc tcgcgcccga tcccccggat gcggcctttc
     5761 tctcacgctg gtgggagggg ttcggcgacc gcgagctcac ggcgctcgtg cacgaggcgc
     5821 tccaggggaa cctcgatctc gcgatcgccc gtgcgaggat acgcgaggcc aaggctcggc
     5881 gggacatctc gatggcgggt ttcctgccgt ttctcgacgc atcggcatcg gtcaccaaaa
     5941 gcgacggcgg ggacggaccc agccggtcct tgtacgacgc ctccctggac gcgcgctggg
     6001 agctcgacct gttcgggggg accaggcgct cggtggaggc tgcccaggcc tcctacgagt
     6061 cctccgagga ggcgttcaac gccgccctcg tgagcctctg cgcagagatc ggcatcaact
     6121 acctcaacct gtgcacctac cgggaacggc tcagggtcgc acgcttgaac gcggcgaacc
     6181 aggaagagac gtacaggctc gcacgcaacc ggtacgaggc cgggctggca gacgccctcg
     6241 acctcgagca ggcaagctcg gcgctcgaga gcacacgggc cggcatcccc gtcctcgagg
     6301 acgcggtcga ggcgtgcctg aaccggctct gcgtgctcac cggcgcggcg ccgggggcgc
     6361 ttagggaacg tctctcgaag acgcccggcc tcccgccctt gccttcgacc gtcgcactgg
     6421 gcgtccctgc ggacatcctc aggcggaggc ccgacatccg cagggcggaa cgcgagctcg
     6481 ccgcccagag cgccagggtg ggcatagccg cctccgatct ctacccgaag ctcacgctga
     6541 acggcaccat acagctgagc gccgagcgat cctcggaact cttctcctcg gggtcgagga
     6601 cgatcggctg ggggccggcg ctgagcgttc ccgtcttcca ctggggcacc ctcaagaagg
     6661 gcgtcgaggt gcaggacgcc ctgctggagc aggcgggcct ctcgtacagg gcgacggtgc
     6721 tctccgcgct ggaggaggtg gaaaacgccg tcagctcgta cgcgaaggag gccgcccgcc
     6781 tggagaacct cgagaaagct caagtctcgg cgcagaaggc cttcgagatc gccaaggcct
     6841 cgtacgcatc cgggaacacg gatttctcgc ggctgctgga ggccgagagg acgctcctgg
     6901 cggcccagga cagcctcgcc cagagcaagg gctccctcgc cgtctcgctc gtgcggctct
     6961 acaaggtgct cggcgggggc tggccgtaca gaccggccgg catggagggg gcccgagggg
     7021 ctcagggctc gtgacgaacg ggaagagggc ccgccgcgcc atcgtgccgc cgggccgggc
     7081 ccgcccggga ggcgggtcac cccgttcagg cggggcgcac ccgaaagcac ggacgattgc
     7141 ggtatgtcag ccggagaggg agtcgagatg gacgagacag ggatcctgaa ggacaaggac
     7201 ggcgagaccc ggggctccgc caaggcacgc cttgcccgcg tgcacggccg aaccgtcaag
     7261 gcgctggcgg ttgtcgccgt cttcgcggcc gccgccatag cgttcttcgc gaggtcggaa
     7321 ggccccgggc agtacgtgta caagacgcag aaggccgcca ggggcgacct cgtggtcacg
     7381 gtgagcgcca ccgggacgct ccagccgctg aatgaggtgg aggtgggcag cgagctgtcg
     7441 ggcatcatcc ggaccgtgga ggcggactac aacgaccgcg tgcgcaaggg ccaggtcctg
     7501 gcgcgcctgg acaccgagaa gctcgaggcg aaggtgctgc agtcgagggc aaccctccag
     7561 accgcccgcg cgaaactcat ggaggccgag gcgaccctcg cggagagcga gagcaccctc
     7621 aagaggctca ggcgcctctt cgagctcacc aagggcgaga gcccctctgc cctcgacctg
     7681 gaatcggcgc aggcagccgc ggagaaggcc cgcgcatcgc tcgcctccgc ccgggcccag
     7741 gtgtcccagg cagaggcgaa tctcagggcc gacgaggcgg acctctccaa ggccctgatc
     7801 cgttccccca tcgacggcgt cgtgctcgag ttcaccgagg acgccgtcg
//
LOCUS       DAOSCR010000035         7835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6006_length_7835_cov_3.381234, whole genome shotgun sequence.
ACCESSION   DAOSCR010000035 DAOSCR010000000
VERSION     DAOSCR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6006_length_7835_cov_3.381234"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..262)
                     /locus_tag="PLT69_01735"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="PLT69_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170527.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; incomplete; partial in the middle
                     of a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            complement(311..787)
                     /locus_tag="PLT69_01740"
                     /pseudo
     CDS             complement(311..787)
                     /locus_tag="PLT69_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     regulatory      complement(855..916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1429..2409
                     /locus_tag="PLT69_01745"
     CDS             1429..2409
                     /locus_tag="PLT69_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOJ13205.1"
                     /translation="MVSALFLTLGVAMLFGGGELLVRGASGVARALRVSPLIIGLTIV
                     AFGTSAPELVVNLVAVLQGSSGVAFGNVIGSNLANIGLILGLAAVFRPIQVDGTVIVR
                     EIPMMILVSAVAVLLGLDGLLQGMPDVFSRSDCLVLLLLFGVFLYYNIFDAVRARRSD
                     RYLEEVEKRIAAPVGGSLLSDMVRTLAGLALLVAGGKLTVMGAVGTARGLGVSEGLIG
                     LTVVAVGTSLPELAASCIAAWRNEPELALGNVVGSNIFNLCFVMGLAGAIAPVQVPGG
                     GALDLTAMLTISLVLMPMALTASRRITRLEGFALVVAYAAYIAFRSLAVG"
     gene            complement(2429..3640)
                     /locus_tag="PLT69_01750"
     CDS             complement(2429..3640)
                     /locus_tag="PLT69_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ13206.1"
                     /translation="MADFYQHPMIATLQRLKDRPIGNMESELELISLKRKMVLLLPAL
                     VSEFDTPSMPRIIDELTQVNYLSKIVLSLDRADWDQFETVKKKLSLLPAEVKVVWHDG
                     PRLQGLYDELRKNDFRLEVPGKGRSVWMTVGYILADRDVDAIALHDCDIMNYSREIVA
                     RLFYPIVHPALDYEFSKGYYARVTDRLYGRVTRLFYLPLITTLKKILRTNTFLEYLGS
                     FRYALAGEFAMISSLARGIRISPTWGLEISLLNEVYQRTAPERICQVEIAETYEHKHQ
                     GLEKDKPDSGLIRMATDIAEALFRILSQDGVVLSQSFFRTMFTTYMEESRMAIEKNNA
                     LSLINGLTYDRHSEIEASEAFVESLKTATANFVSDPVGIPMMSAWTRISAAIPDFQER
                     LYHAVEEDNRM"
     gene            complement(3654..4898)
                     /locus_tag="PLT69_01755"
     CDS             complement(3654..4898)
                     /locus_tag="PLT69_01755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ13207.1"
                     /translation="MTTARRFSTALRDYAREKIERLGSAEIVVGIPAYLCDDSIVNVI
                     KTIRKGLDTHFHGAKALIMVSDGGSTDDTRDAAMAYDVKSFNIEKIITIYRGLPGKGS
                     AIRAIFEAAVFLKPKAIALFDSDLVSITPLWIKNILDPVMNGYDFVAPDYIRYKFDAT
                     ITNSIAYNLTRALYGRRIRQPIGGDFGVSPRLAKYYLDQEDVWETDVARFGIDIWMTT
                     TAIVGGFDICQARLGVKVHGEKDPAADLGPMYRQVVGTSFRLMEMYQDFWKKVKGSRD
                     IPSLGEYPLEEPAPFDINQDDLVEYFQTGYFNFNGVWRQILEEKDMAVYEGLVNSTSR
                     DFFLPIDTWVRTVYRCAAAFHSTPRQRMKVLNTMIPLYYARVASLVNELKDRDGPLAE
                     ELFQEQAGVFEDMKGYLLDIWQ"
     gene            complement(4949..5641)
                     /locus_tag="PLT69_01760"
     CDS             complement(4949..5641)
                     /locus_tag="PLT69_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOJ13208.1"
                     /translation="MCTSKTRFELETVLVSISPESPFIVENGGGIFFPPACSEWIIPD
                     ARPVGRLRCVSLGAPYAFIRSFMDRVGKRFSARGFADMGVDEVSLLTGLDPEEARLAR
                     IREFSEPFVIERETDLSGLEAEAAASGLVITRGGRFYHLVGKDTDKGVAVRRVIKTVS
                     HNLGRDIVSIAIGDSPNDFPMLEAVDIPVLIPHEDGSIEGVDLPGLVRATRPGSKGWN
                     DAVMEILHTIEL"
     gene            6237..6638
                     /locus_tag="PLT69_01765"
     CDS             6237..6638
                     /locus_tag="PLT69_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ13209.1"
                     /translation="MFNLKFQEVIGLLLGPEAHGERIGVFHPLIDVYENADGLCIEAE
                     IPGVCPEDVSVEVMGRSIVISGTKKDAVTNRGVRYIRMERGFGKFTREFEIPERFNLE
                     QVDARFMDGVLKIRIARIEEGVKKVKRIEIS"
     gene            6649..>7835
                     /locus_tag="PLT69_01770"
     CDS             6649..>7835
                     /locus_tag="PLT69_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOJ13210.1"
                     /translation="MENEKQTLEIPENIPLLPIRDVVIYPYMILPLFVGRGLSIKAVD
                     EALNKDRYIFLAAQKDSSIEEPTEDQIYTVGTVAMVIRMLKLPDGRVKILVQGVAKAR
                     VKRFIREEKGFYSVDIEKIDEPEVKEITVEVEAMMRTVKEQSERILQLRGIVSPDALS
                     ILEAIDDPGRLADLVASNLRLKVEESQHVLEIIDPVERLRHVNQLLSKEMELSEVQAR
                     IQSQAKEEMSKTQREYFLREQLRAIQQELGEVDEKSKEIEEYLEKINNAGMPEDVKKE
                     AVKQLDRLKMMHQDSAEANIIRTYLDWMVDLPWSRSTQDRLDIKAASKILDEDHYGLD
                     KVKQRILEYLAVRKLNPEKKGAILCFVGPPGVGKTSLGRSIARALGREFYRLSIGGVR
                     DEA"
BASE COUNT         1454 a         2324 c         2530 g         1527 t
ORIGIN      
        1 gcgcgcggtc gtcgccgcgg gcgctgccgg tgcccatcat cgccaggccc atctccgaca
       61 tcaccgtgcg cacgtcggcg aagtccacat tgatcaggcc cggacgcacg atcagatcgg
      121 cgatgccctg caccgcgcct tgcagcacgt cattggcggc gcggaacgcc tggatcatgg
      181 tggcattgcg gccgagcacg gtgatcagct tttcattggg aatcgtgatc aacgaatcgc
      241 agtgctgcga cagctcctcg atgaggtcaa ggtcgttctc tgacagcggc cggtccgagc
      301 tcacggccaa gatcgccgct cccacctccg ggagccagtt ccgagaggct tcacggtgat
      361 aggtgaacac gctccccagg ccgggcgtat ccaccaggcg cagcccaggg tagggctgga
      421 gagacgggag ctcgatatcc accatgtcca cctgtttctc gttggatggg ttctccgtct
      481 cggagatgta ctgacccaac tcctcgagcc cgacatcggt ccgggtgccg tccaggaagg
      541 tcacggttgc atgctcaggc tctccgtaag acaggcgcgt gatcaccgtg gtgaccggga
      601 tgacgccgac aggcaggatg tcacggcctg tgaggctgtt gatgaacgag ctctttcccg
      661 ccttgaactg cccgaagacg gcgatgtcga tgaggggagg tcgggcgagc aggttctcgc
      721 agatctcgat ctggcggtgc agggaggcga tgcggaaccg atcggcggcc tcacggacgc
      781 tgtccataag tctcctggcg tcaacgttcg aatgcatagg cggtgcggcg accatgcgtg
      841 gctcctttca gatctctctg tcaggagtca tcagcctgca ggcggttaca ggggggactc
      901 catccccgct cgggtgcctt cttctctacc gcagcccgtg cgaggctgtc aatggagtcg
      961 atgcagatca cggttaccag aaggccctga tcatttttca atccgtgcaa gcgatgttct
     1021 cttggtgcgg ggttctggaa aggcctggcg agttggatga agaggcggta cggcgctttt
     1081 ctccatcatc ctcatctttc cgggaaacgg cgctgagacg aaacggagaa agaacaggga
     1141 gacgatgggc atcacgggca cggacgtcgc caaggaggga tccgacacgg tggtcgtccg
     1201 gcaggtggga ggcaacggct gaggtgtatg ctgctctggc catcaggatg acgttcgggg
     1261 gtttgcccgt gtattccggc atacccttga ctatgcccga gatcctcgta aacagggacg
     1321 attccctgcc ttcccgtcga ttccctgttc gagcggctcg ttttcggcac ctggcatggc
     1381 gcatttatct gttgatttcc accagaaaat cttcaaagct ctctggacat ggtctctgca
     1441 ctgtttctca ctctcggcgt cgccatgctc ttcgggggag gggagctcct cgtcaggggc
     1501 gcctcaggcg tggcgcgggc cttgcgggtc tcgccgctca tcatcgggct caccatcgtg
     1561 gccttcggca cgagcgcccc ggagctcgtg gtgaacctcg tggccgtgct ccaggggagc
     1621 agcggcgtag ccttcgggaa cgtgatcggg tcgaacctgg ccaacatcgg cctcatcctg
     1681 ggcctggccg ccgtgttcag acccatccag gtggacggca cggtcatcgt ccgggagatc
     1741 cccatgatga tcctggtctc cgcggtggcg gtgctcctcg gtctcgatgg attgctgcag
     1801 ggcatgccgg atgtcttctc gcgttccgac tgcctcgtgc tcctgctcct cttcggggtc
     1861 ttcctctact acaacatctt cgacgccgtg cgggcgagac gttccgaccg ttacctcgag
     1921 gaggtggaga agcgcatcgc cgcacctgtg ggaggatccc tgctctcgga catggtgagg
     1981 accctggccg gcctggccct gctcgtggca ggcggcaagc tcaccgtgat gggtgcggtg
     2041 gggacggcca ggggcctcgg ggtctccgag ggcctcatcg ggctcaccgt ggtggccgtg
     2101 ggcacctccc tgccggagct cgcggcctcc tgtatcgccg cctggaggaa cgagccagag
     2161 ctggccttgg gcaacgtggt ggggtcgaac atcttcaacc tgtgctttgt catgggactt
     2221 gcgggcgcca tcgcaccggt gcaggtcccc ggcggcgggg ccttggacct tacggccatg
     2281 ctcacgatat cgttggtgct catgcccatg gcgttgacgg cgtccaggag gatcacgaga
     2341 ctggaggggt tcgccctcgt tgtcgcgtat gcagcctaca tcgccttcag gtctctggcc
     2401 gtagggtgag ggcgcccgag ggtatgggct acatcctgtt gtcttcctcc actgcgtggt
     2461 agaggcgttc ctggaagtcc gggattgcgg cgctgatcct ggtccaggca ctcatcatgg
     2521 ggatgcccac ggggtctgac acgaagttgg cggtggccgt cttgagcgat tccacgaatg
     2581 cctcggaggc ctcgatctcg ctgtggcggt cgtacgtgag gccgttgatg agcgacaggg
     2641 cgttgttttt ctcgatggcc atgcgcgact cctccatgta ggtcgtgaac atggtcctga
     2701 agaatgactg gctgaggacg accccgtcct ggctcagtat cctgaagagg gcctccgcga
     2761 tgtcggtcgc catccgtatg aggccgctgt ccggcttgtc cttttccagg ccctggtgtt
     2821 tgtgctcgta ggtctcggcg atctccacct ggcatatcct ctcgggcgcg gtcctctggt
     2881 agacctcgtt gaggagcgag atctccagcc cccacgtggg cgatatcctg atgccgcggg
     2941 cgagcgagga gatcatggcg aactcgcctg ccagcgcgta gcggaaggaa cccaggtact
     3001 cgagaaaggt gttcgttctg aggatctttt tcagggtggt gatgagcggc aggtagaaga
     3061 gcctcgtgac cctcccgtag agacggtcgg tcacccgggc gtagtacccc ttgctgaact
     3121 catagtccag ggcggggtgg acgatggggt agaacaggcg ggcaacgatc tcgcgcgagt
     3181 agttcatgat gtcgcagtcg tggagggcta tggcgtccac gtcgcggtcc gccaggatgt
     3241 agccgacggt catccagacg gatctcccct ttcccggtac ctcgagcctg aaatcgttct
     3301 tcctcagttc atcgtagagg ccctggagcc tcggcccgtc atgccagaca accttcacct
     3361 ctgcaggcag gagggacaac ttcttcttga ccgtctcgaa ctggtcccag tcggccctgt
     3421 cgagggaaag gacgatcttg gagagatagt tcacttgggt cagctcgtcg atgatgcgag
     3481 gcatggacgg ggtgtcgaac tcgctcacga gcgcgggcag caggagcacc atcttcctct
     3541 tgaggctgat gagttccagt tccgactcca tgttcccgat aggcctgtct ttcagtcgct
     3601 gcaacgtcgc gatcatgggg tgttggtaga agtctgccat ggtcagggtc tccttactgc
     3661 cagatatcga gcaggtatcc cttcatgtct tcgaacacgc ccgcctgctc ctggaacaat
     3721 tcctcggcca ggggcccgtc tctgtccttg agctcgttca ccagtgaagc cacgcgcgcg
     3781 taatacaggg gtatcatcgt gttgaggacc ttcatgcgct gcctgggcgt ggagtggaac
     3841 gcagctgcac agcggtacac cgtgcgcacc caggtgtcga tgggcaggaa aaagtctctg
     3901 gatgtgctgt tgacgaggcc ctcgtagacc gccatatcct tttcctcgag gatctgacgc
     3961 cacacgccgt tgaaattgaa atacccggtc tggaagtact ctaccaggtc gtcctggttg
     4021 atgtcgaagg gggcgggctc ctcgaggggg tactctccca gcgaggggat gtcgcgggag
     4081 cccttcacct tcttccagaa gtcctggtac atctccatga ggcggaagct cgtgcccacc
     4141 acctggcggt acatggggcc caggtctgcg gcagggtcct tctcgccgtg gaccttcacc
     4201 ccgagcctgg cctggcagat atcgaacccg cccacgatgg ccgtggtggt catccagatg
     4261 tcgatgccga accgggccac gtcggtctcc cagacgtcct cctggtcgag gtagtacttg
     4321 gcgagccggg gggagacccc gaagtcgccg ccgatgggct ggcggatgcg gcgcccgtac
     4381 aaggccctgg tgaggttgta cgcgatggag ttggtgatgg tggcgtcgaa cttgtaccgg
     4441 atgtagtccg gcgcaacgaa gtcatagccg ttcatgaccg ggtcgaggat gttcttgatc
     4501 cacaggggcg tgatggagac gaggtccgag tcgaaaaggg cgatggcttt gggcttgaga
     4561 aacacggccg cctcgaagat ggccctgatg gccgagccct tgcctggcag gccccggtag
     4621 atggtgatga tcttctcgat gttgaacgac ttgacgtcgt aggccatggc cgcgtccctc
     4681 gtgtcgtccg tggagccgcc gtcggagacc atgatgaggg ccttcgcccc gtgaaagtgg
     4741 gtgtcgagac ccttgcggat ggtcttgatc acgttgacga tggagtcgtc gcacaggtac
     4801 gcggggatac cgacgacgat ctcggcgctg ccgagcctct cgatcttctc ccgtgcatag
     4861 tccctcaggg cggtggaaaa cctcctcgcc gtcgtcatac gacctccttt gtgtcgtccc
     4921 ctccggcccg gggaagcggt gtgccctgct acaactcgat ggtgtgcagg atctccatca
     4981 ccgcatcgtt ccatcccttg cttcccggac gggtcgccct cacgaggccc ggcaggtcga
     5041 caccctcgat ggacccgtcc tcgtgcggaa tgagcacggg gatgtcgact gcctcgagca
     5101 tgggaaagtc gttggggctg tcccctatcg cgatggagac gatgtctctc cccaggttgt
     5161 gcgaaacggt cttgatgacg cgcctgacgg caacgccctt gtccgtatcc tttcccacca
     5221 ggtggtagaa cctgccaccc ctggtgatca cgagccccga tgcggcagcc tccgcctcga
     5281 ggcctgacag gtcggtttca cgctcgatca cgaacggttc ggagaactcc cggatgcgcg
     5341 cgagcctggc ttcttcggga tccaggcccg tgagaagcga cacctcgtcc acgcccatgt
     5401 ccgcgaaccc cctcgcgctg aaccgtttgc ccacacgatc catgaaggac ctgatgaacg
     5461 cgtacggcgc gccgagcgac acgcacctca gacgcccgac gggccgtgcg tcggggatga
     5521 tccattcact gcatgcaggg gggaagaata tcccgccccc gttctccacg atgaaggggc
     5581 tctcgggtga gatcgagacg agcactgtct ccagctcgaa acgtgtcttg ctcgtgcaca
     5641 cgacgagggc accgccttcg atcgcagggc ctcgagcgca tgccgcgcac cgtcgatcga
     5701 gtaatccgcg tggtccatca acgaaccgtc gaggtccgtg aacacgatgg gcatccgtcg
     5761 cccgcgcatg gtagggttga gggccatggg tcgagagaat cgcttcctgg atggattgaa
     5821 tgctctcaat aatacgtttc gggcatgggt ctgtcaacct cgccccaagc ggcaccggcc
     5881 atggccaccg cctgccgagc gggtgagcgc attgatgacc gtcggaagcc cttgtgtgaa
     5941 acccttcacg atacgcccgt gcagagcgcc tgtgtgcacg atcgtcactc catgaacgca
     6001 tggagcgcag tccatgtgag gggcagggtc cccgccgggg gggagtgcca gggtcgggtg
     6061 caactcgcct gcggttgagg agaccgggaa aggcgaaatc gcatacacgc gatcaggaca
     6121 aaacagagga ggaggtccgc agccacgggg caaacggccg catcagggac tcgagcttga
     6181 cttgccggaa tccgatccta taaaggtact gatgattaca gacactcgag gtcttcatgt
     6241 tcaatctcaa atttcaggaa gttatcggtc tgcttctggg tccggaggcc cacggcgagc
     6301 gcatcggcgt gttccatccc ctgatcgacg tatacgagaa cgctgatggg ctgtgcatcg
     6361 aggccgagat accgggggtg tgccccgagg acgtgagtgt ggaggtcatg ggcaggtcga
     6421 tcgtgatatc gggaaccaag aaggacgccg tgaccaaccg cggggtgagg tacatacgca
     6481 tggaacgggg cttcggcaag ttcacgaggg aattcgagat accggaacgg ttcaacctcg
     6541 agcaggtgga cgcccgtttc atggacgggg tcctcaagat acggatcgcc cgcatcgaag
     6601 aaggggtgaa gaaggtcaag aggatagaga tatcctagga gaaacttgat ggagaacgag
     6661 aaacagacgt tggagatacc agagaacata ccccttttgc ccatcaggga cgtggtgatc
     6721 tacccgtaca tgatcctgcc cctgttcgtg ggcaggggtc tgtccatcaa ggccgtcgac
     6781 gaggccctca acaaggacag gtatatcttc ctcgccgcgc agaaggactc gagcatcgag
     6841 gaacccacgg aagaccagat ctacaccgtg gggaccgtcg ccatggtgat ccgcatgctg
     6901 aagctgcccg acgggagggt gaagatcctc gtccagggcg tggccaaggc gcgcgtgaag
     6961 agattcatca gggaagagaa ggggttctac agcgtcgata tcgagaagat cgacgagccc
     7021 gaggtgaagg agataaccgt cgaggtcgag gccatgatgc gcacggtgaa ggagcaatcc
     7081 gagcgcatcc tgcagctcag gggtatcgtg tctcccgacg cactgtccat actcgaggcc
     7141 attgacgacc cgggcaggct cgccgacctc gtcgcctcca acctcaggct caaggtcgag
     7201 gagtcccagc acgtcctcga gatcatcgac ccggtcgaga ggctccgcca cgtcaaccag
     7261 ctcctctcca aggagatgga gctctctgag gtccaggcga ggatacagtc ccaggccaag
     7321 gaggagatga gtaagaccca gagggaatac ttcctccgcg agcagctcag ggccatccag
     7381 caggaactcg gcgaggtgga cgagaagagc aaggaaatcg aggaatacct cgagaagatc
     7441 aacaacgccg gcatgcccga ggacgtcaag aaggaggcgg tgaagcagct cgaccgcctc
     7501 aagatgatgc accaggactc ggccgaggcg aacatcatcc ggacctacct ggactggatg
     7561 gtcgatctcc cctggagcag gtccacccag gaccggctcg acatcaaggc ggcctcgaag
     7621 atactcgacg aggaccacta cggtctggac aaggtcaagc agaggatcct cgagtacctg
     7681 gccgtgcgga agctcaaccc cgagaagaag ggcgcgatcc tgtgcttcgt ggggcccccg
     7741 ggcgtcggca agacctccct cggccgctcc atagccaggg ccctggggcg tgagttctat
     7801 cggctctcca tcggcggcgt ccgcgacgag gccga
//
LOCUS       DAOSCR010000036         7817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6022_length_7817_cov_3.176243, whole genome shotgun sequence.
ACCESSION   DAOSCR010000036 DAOSCR010000000
VERSION     DAOSCR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6022_length_7817_cov_3.176243"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLT69_01775"
     CDS             complement(<1..1055)
                     /locus_tag="PLT69_01775"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HOJ13211.1"
                     /translation="MRSTIYNGYVEHTRFRPAFHHFRFRLYFYCLDLDELSVLDRTLP
                     LFGYNRARPVSIHDSDYLDSGPGSIREKLLTYLGDTLASRVDRVLLVTQPRYFTAVFN
                     PVSFYYCLARDGSLLCTVAEVNNTFGERHVYVLDKRHGPQAGYPAAFVTDKAFHVSPF
                     NALDGAYVLTFSEIGPELTISVDLMRNGERFFTATLTGRRSELTTVNQVMLMTRHPLV
                     PKLTMARIYGEAAKLFFLKKLHYHPKPAPASPMTISKAPPGLAERIFFRVIDGLLDKI
                     ERGRLRIHLPDGRTKIYGGTDVHGPDAEIRVNDYAFFSRVALNGEIGLGEAYVDGLWD
                     SDDLTKTLELLIVNRRA"
     gene            complement(1096..2325)
                     /locus_tag="PLT69_01780"
     CDS             complement(1096..2325)
                     /locus_tag="PLT69_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ13212.1"
                     /translation="MDSGQVRTLAVVGGGVAGIVASYLLQRRFEVSLFEKNDYIGGHT
                     HTIVIDEGPDAQTPVDTGFIVFNDKTYPLFNRFLAQLRVEKQKSSMSFSHFDARSGMM
                     YASTNMDTFLAQRSNLFRPSFWLMSLAVLRFNHITKNHLEEGKLRGLTLGEFLSVHRF
                     NRYFVEQYLIPICASVWSAPDVRMLEFPMETFARFFLNHGLLSVVNQPQWYVVKGGSH
                     TYVNAFLKTFRGRVYPATPVAAVTRGDAGVTLKTVDGTEHPFDAVVLACHADESLRLL
                     ADAHPDERRLLGAWEYSRNRTILHHDTSFLPPMQKVWASWNYMRMEAVESGSPVTLTY
                     YMNRLQGFSTRRPYCVTLNPKKPIQDGAIVRDMIYHHPVYTRESMETQRGIAGMNGQK
                     STYFAGATSAMDSTRTR"
     gene            2562..3044
                     /locus_tag="PLT69_01785"
     CDS             2562..3044
                     /locus_tag="PLT69_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ13213.1"
                     /translation="MKIDLAHLERMKGGIFSFLMRLSGDYELSRDILQETFLRYLERY
                     DGKDTQPSLLFTIARNAFIDHVRKSGRELPLVDDHRDCAPDAHERFRVRQEYSRVLEA
                     IESLGQDEREILSMALSGDLSYRQIADIVGTSEANVKVKVHRARCRIREILKGGDHER
                     "
     gene            3034..3612
                     /locus_tag="PLT69_01790"
     CDS             3034..3612
                     /locus_tag="PLT69_01790"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOJ13214.1"
                     /translation="MRDELISMYIDDELDLDDKREFVELIHHDRTFKDRTMEFLDLEK
                     ELRSEVVEEAPEVVMATTGVLPLVRRRRLQIAAAAVAAVAAIVAVFVLRPESPRVDGK
                     VAQGSYRFVIYQPDISRAEIMGDFTGWKRVPMERVSMSGYWETSLPVEPGEHRYVFVL
                     DGEKRIADPTVISREKDDFGSENSILVVRVGT"
     gene            3609..3935
                     /locus_tag="PLT69_01795"
     CDS             3609..3935
                     /locus_tag="PLT69_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13215.1"
                     /translation="MMRLAAVFVGLALLVTGCTHHGVHIEGDRVILTLEAPHARDVLF
                     ASSLDGFVLHEATRVHGSTWEASMPAGSPFSYFYLVDGEVYLPDCPYAELDGFGSANC
                     VYVPGM"
     gene            3987..5003
                     /locus_tag="PLT69_01800"
     CDS             3987..5003
                     /locus_tag="PLT69_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13216.1"
                     /translation="MKKTLGAIAAILLVSTTALADAVSEELGERVTLQVMTSTREMIR
                     LGIPEGDAVRLTRQMIQNRYKEQEILAAHEVIRQMVRQGLPVRPAMDKAYEGMAKHVG
                     ARDTVRAMEKVRLRYLFAYGQAEEILGQIRERSMLGDVIAECLAAGMAEGDVTAIQLR
                     LKTRSRTMTRDQALQLSLQSFMAAREMSRSGAGGTASSGVVCRALERSWSAQDMERLR
                     LTFMSQVRLGNPSGLAARYENQIKSGVNADGLGRHGAGSAQQGFGDGQGRLGSGGSGE
                     SGPGTGGASGNGSSGSGSGGSGGSGGAGGGSGSSGGSGGGDHAGGGSGPGGGGTGRGG
                     RGSH"
     gene            complement(5221..5895)
                     /locus_tag="PLT69_01805"
     CDS             complement(5221..5895)
                     /locus_tag="PLT69_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ13217.1"
                     /translation="MNTLIHIVDDEPDIIQLVSIHLKKAGFAVQGFPEGESFLKSLAK
                     ARPALIILDLMLPDMDGFEICKLLKSDEHTADIPVIMLTAKGTETDKVEGLELGADDY
                     VTKPFSPKELVARVHSVLRRAGKPCGVLNVGHGVVIDPDRFEVTIDGACVNLTAVEFK
                     ILLFLASNRGMVLSRERILDHLWGNEKAVLDRTVDVHIKNIRAKLGQAGNIIKNVRGV
                     GYKLQP"
     gene            6057..7223
                     /locus_tag="PLT69_01810"
     CDS             6057..7223
                     /locus_tag="PLT69_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13218.1"
                     /translation="MMNRTILAFLTCVSLAVSSGVHAASFKLGDVDMTLGGSARLDVG
                     WQSSDYGDVAQGKPDSMTDFFLRNPGNSRVKIKAAYEGITGYAEIGLKTDNSVGVRHV
                     YASYDLGEGRGILFGQTDTVFSELRPDQRLNDDLNLQGFGYLYNNRRPQVRYTVASGA
                     VAFKVAIEEPRGVADEYVSVSGDHLKEKAMPAVAAAFEYKDGGISVSPSAFYQQFRNK
                     ANDDNPDTKDITVATYGLCLSGSVKADPATFTGGLWYGQNLSVFDLDKRKNSPSTVFG
                     KPVADEAGNDIEDIDSLGGWAQVSLKAGPGVFRAGAGMQRSETGWKGDAYEDDISTMG
                     AFVNYEYTVAKGFTVTPEIAYFDYGKDANKNKVSEGKSDLGSDTLVGVHLQYDF"
     gene            7346..7789
                     /locus_tag="PLT69_01815"
     CDS             7346..7789
                     /locus_tag="PLT69_01815"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOJ13219.1"
                     /translation="MDKARVLFLCTGNSARSQMAEAFLRTYAPHAFEAYSAGLEPKGV
                     HPLTVEVLDELGIDIRSQRSKHLDEYMGKMFFGYLITVCSQADASCPAVFPGVGVRMH
                     WAFDDPAACRGTQAECLQEFRRVRDEIRNRVLSWLEELHVEPART"
BASE COUNT         1534 a         2247 c         2581 g         1455 t
ORIGIN      
        1 aaggcgcgac ggttcacgat caggagttcc agcgtcttgg tgagatcgtc gctgtcccac
       61 agcccgtcca cgtacgcctc gcccaggccg atctcgccgt tcagtgcgac acgtgaaaag
      121 aaggcgtagt cgtttaccct gatctcggca tctggcccat ggacgtcggt accgccgtag
      181 atcttcgttc tcccgtccgg caggtgaatc ctcaggcgac cccgctcgat cttgtcgagc
      241 agcccgtcga tgacccgaaa gaagatgcgc tcggcaagcc cgggcggggc tttgctgatg
      301 gtcatggggc tcgcaggcgc cggctttgga tggtagtgga gcttcttgag gaagaacagt
      361 ttcgccgcct cgccgtagat ccgggccatg gtgagtttgg ggacgagggg gtgcctggtc
      421 atgagcatga cctggttcac cgtcgtgagc tcagagcggc gtcctgtcag ggttgcggtg
      481 aagaatctct cgccgttgcg catgagatcg acgctgatgg tgagctccgg cccgatctcg
      541 gagaaggtga gcacataggc accgtcgagg gcgttgaaag gcgagacgtg gaaggccttg
      601 tccgtgacga aggccgcggg gtagcctgct tgcgggccgt ggcgtttgtc gagaacgtag
      661 acgtggcgtt cgccgaaggt gttgttgact tcggcgacgg tgcacagcaa ggacccgtcc
      721 cgtgcaaggc agtagtagaa gctcaccggg ttgaacacgg ccgtgaagta ccgcggctgg
      781 gtcacgagta agacacggtc cacccgcgag gccagcgtgt cgcccaggta agtgagcagc
      841 ttttccctga tggatcccgg accgctgtcg aggtagtcgc tgtcatggat ggataccggc
      901 ctggcgcggt tgtatccgaa cagcggcagc gtcctgtcca gcaccgaaag ttcgtcaagg
      961 tcgagacagt agaagtagag cctgaacctg aagtgatgaa aggccggcct gaatctggtg
     1021 tgctccacgt acccgttgta tatcgtcgat ctcatagctc gatcccgaaa atccgcgcca
     1081 cagcggcggc ggacctcacc gcgtcctcgt ggaatccatt gccgaggtag ctcccgcaaa
     1141 atacgtgctt ttctgcccgt tcatccctgc tatccctctc tgggtctcca ttgactcgcg
     1201 cgtgtagacc gggtggtggt agatcatgtc gcgcacgatc gccccgtctt ggatcggctt
     1261 cttcgggttc agggtcacac agtacggtct cctcgtcgag aacccttgga gcctgttcat
     1321 gtagtaggtg agcgtgactg gggaccctga ctccacggcc tccatgcgca tgtagttcca
     1381 ggacgcccac accttctgca tgggaggcag gaacgacgtg tcgtgatgga ggatggtcct
     1441 gttccgtgag tattcccatg cgccgagcag cctcctctcg tccgggtgcg catcggcgag
     1501 cagccgcagg gactcgtccg cgtgacaagc cagcacgacc gcatcgaagg ggtgctcggt
     1561 tccgtccacg gtcttcagtg tgacgcccgc gtcccccctc gtcacggctg cgacgggggt
     1621 tgcggggtac accctgcccc tgaaggtctt gaggaaggcg ttcacgtagg tgtggctgcc
     1681 gcccttgacc acgtaccact gaggctggtt cacgacggag agcagcccat ggttcaaaaa
     1741 gaagcgggca aaggtctcca tgggaaactc cagcatgcgc acgtccgggg ccgaccacac
     1801 cgatgcgcag atggggatga ggtactgttc gacgaaatac ctgttgaaac ggtggacgga
     1861 aaggaactcg cccagggtga gccctcgaag ctttccctcc tcgaggtgat tcttggtgat
     1921 atggttgaac ctgagaacgg ccagggacat caaccagaag gacggcctga acaggttcga
     1981 acgctgcgcg aggaacgtgt ccatgttcgt gctggcgtac atcatgccgc ttcgtgcgtc
     2041 gaagtggctg aaggacatgc tgctcttctg tttctcgacg cgaagctgcg caaggaatcg
     2101 attgaagagg gggtaggtct tgtcgttgaa tacgatgaaa ccggtatcca ccggcgtctg
     2161 tgcgtctggc ccctcatcga tgacaatggt gtgggtgtgg cccccgatat agtcgttctt
     2221 ctcgaagagg ctcacctcga acctcctctg gaggagatac gaagccacga taccggcgac
     2281 cccgccaccg acgacggcta gtgtcctgac ctgtccggaa tccacggaac cccctcccaa
     2341 accctctggc agggcaatat aatggattaa taatgaccag gtcccggcca ggcaacccga
     2401 agcagggcga tggtgagaac ggtgccttga gacccgccgc ggcggtgggt cggtgggtgc
     2461 cctgaaggtc cggggggcgg acatgggagc gtgcacaaga acagcgccgt cgaacgggga
     2521 acgtgtaacc atttcccctt tcgatcgttc aagggatgtc aatgaagatc gaccttgccc
     2581 atcttgagcg gatgaaaggc gggatattct cgttcctcat gcgcttgagc ggcgattacg
     2641 agctttcgag ggacatactc caggagactt tcctgaggta tctcgagcgg tatgacggca
     2701 aagacacgca accctcgctc ctcttcacca tagcgcgcaa cgccttcatc gaccatgtgc
     2761 gcaagtcggg tcgtgaacta cccctcgttg acgatcacag ggattgcgcg cccgatgcgc
     2821 atgaacgctt ccgcgtgcgg caggaatact cgagggtgct cgaggccatc gagagcctgg
     2881 ggcaagacga gcgggaaatc ctctcgatgg cgctcagcgg cgacctttca taccggcaga
     2941 tcgcggatat cgtgggaacg agcgaagcca acgtcaaggt gaaggtgcac agggcacggt
     3001 gcaggatacg ggagatcctg aaggggggag accatgagag atgaactgat aagcatgtac
     3061 atcgatgatg agctcgacct cgacgacaag agggagttcg tggaactcat ccaccacgac
     3121 aggacattca aggaccgcac catggaattt ctcgatctgg agaaggagtt gcgctcggag
     3181 gtggtcgaag aagcgcccga agtggtgatg gccacaaccg gcgtgctccc cctcgtacgg
     3241 agacgccgcc tgcagatcgc cgccgcggcg gttgcggccg tcgcggcgat cgtcgcggta
     3301 ttcgtgctcc gcccggaaag cccccgggtc gatgggaaag tcgcacaggg atcgtaccgg
     3361 ttcgtcatct accaacccga tatctcccgg gccgagatca tgggggactt caccggctgg
     3421 aagagggtgc ccatggagcg cgtctcgatg agcgggtact gggagacgtc gcttcccgtc
     3481 gagcccggag agcaccgcta cgtgttcgtc cttgacgggg agaagcgcat cgcggatccc
     3541 accgtcatct cccgggagaa agacgacttc ggttcagaga actccatact ggtagtgagg
     3601 gtggggacat gatgcggctc gcagctgtgt tcgtgggcct ggcgctcctc gtcacagggt
     3661 gcacgcacca cggggtccat attgagggag accgtgtgat cctgaccctc gaggcccccc
     3721 atgccaggga tgtcctgttc gcgtcgtccc tggacggctt cgttttgcac gaggcgacac
     3781 gagtccacgg gtcgacatgg gaggcgagca tgcctgcagg ctcaccgttc agctacttct
     3841 acctcgtgga cggggaggtc tacctcccgg actgcccata cgcggagctc gacgggttcg
     3901 ggtctgcaaa ctgcgtctac gtgccgggca tgtaaccttt tacctgcacg ttcgttgtaa
     3961 caccatcgat cgaaaaggag agatccatga agaagacact cggtgcgatc gcggccatcc
     4021 tcctcgtgtc caccaccgcg ctggcggatg cggtaagcga ggaactggga gagagggtca
     4081 ccctgcaggt gatgacgagt accagggaga tgatcaggct cggcataccg gaaggcgatg
     4141 ccgtcaggct cacccggcag atgatccaga accggtacaa ggagcaggag atcctcgctg
     4201 ctcacgaggt gatccggcag atggtccgcc agggcctccc ggtaaggcct gccatggaca
     4261 aggcctacga gggcatggcc aagcacgtgg gcgctcggga cactgtccgg gccatggaga
     4321 aggtgaggct gagatatctt tttgcttacg ggcaggccga ggagatcctc ggacagatca
     4381 gagagcgctc tatgttgggg gatgtcattg cagaatgcct ggcggcaggc atggcagagg
     4441 gtgacgttac ggccatacaa ctcaggctga agaccagaag caggaccatg acccgggatc
     4501 aggcgctgca gctctccctc cagagtttca tggctgctcg cgagatgtcc aggagcggcg
     4561 ccggcggaac cgcgtcctcc ggtgtggtct gccgggcgct cgagcgttcg tggagcgcac
     4621 aggacatgga gcggctgcga ttgaccttca tgagccaggt gagactcggg aacccctcag
     4681 ggctcgcggc gaggtatgag aaccagatca agagcggggt gaacgccgac ggccttggca
     4741 ggcatggggc gggctccgca cagcagggct tcggtgacgg gcagggtcgc ctggggagcg
     4801 gtgggagcgg tgagtcgggg ccgggcaccg gcggtgcatc aggcaacggc tcttccggta
     4861 gtggttccgg cgggagcggc ggcagcggcg gggcaggagg tggaagcggc tcgtcaggcg
     4921 gctccggcgg cggtgaccat gcaggcggcg ggtcaggtcc cggcggaggt ggaacaggtc
     4981 gtggtggccg tggatctcat tgagagaccg agagccttca ggcccctcga ccgccagatg
     5041 caggcgggct gaagggacgg cttgcgtgca ggtgtaccag ggattctggg ggtcctgaag
     5101 agccaccctc gatgaggacg tgcacctctc gagattgagc gctcgatctc gagagggagg
     5161 gcgtggcgct cggggcgttg caagtgctgc ccaggaatgt cgcgagggag accgggctgc
     5221 ttacggctgg agtttgtacc cgacgccgcg cacgttcttg atgatgtttc ccgcttgccc
     5281 gagcttggcc ctgatgttct tgatgtgcac atccaccgtc ctgtcgagca cggccttctc
     5341 gttgccccac aggtggtcga ggatgcgctc gcgggaaagg accatgcccc tgttggaggc
     5401 gaggaaaagg aggatcttga actcgacggc ggtcaggttg acacaggccc cgtcgattgt
     5461 cacctcgaag cggtcggggt cgatcacgac gccgtggccc acgttcagga cgccgcaggg
     5521 ctttcccgcc ctgcgcagga ccgagtggac ccgcgccacg agttccttgg gcgagaaggg
     5581 cttggtcacg tagtcgtcgg ccccgagctc gagcccctcg accttgtccg tctccgtccc
     5641 cttggccgtg agcatgatga cggggatgtc cgcggtgtgc tcgtccgact tgaggagctt
     5701 gcagatctcg aacccatcca tgtcgggcaa catgaggtca aggatgatga gggccggccg
     5761 cgccttggcc agtgatttca ggaacgattc gccttcggga aagccttgaa cggcaaagcc
     5821 cgccttcttg aggtggatgc tcaccagctg gatgatgtcc ggttcgtcgt ccacgatgtg
     5881 gatgagcgtg ttcatggctc tttaccgtct ctcgctgtca tttcttacca aagaccgcga
     5941 ggatcgatcc agcccaaaac ggcccttcac gcatttttca tatgcctttc acacggcctt
     6001 tacgggaaac acataggtct tgcctcagca gctacccatc gagacaggag gaatgaatga
     6061 tgaaccggac aatcttggcg tttctgacct gtgtgtcact ggcggtgtct tccggcgtcc
     6121 atgcggcgag cttcaagctc ggcgacgtgg acatgacgct gggcgggagc gcccggctgg
     6181 atgtggggtg gcagtcgtcc gactacggcg acgtcgcgca gggaaaaccc gactccatga
     6241 cggacttctt cctgagaaac cccgggaaca gcagggtcaa gatcaaggcc gcctacgaag
     6301 ggatcaccgg gtacgcggag atcggcctga agacggacaa ctcggtcggc gtgagacatg
     6361 tctacgcctc ctatgacctg ggagagggcc gcgggatact cttcggccag accgacacgg
     6421 tgttctcaga gttgaggccg gaccagaggc tcaacgacga cctgaacctc cagggtttcg
     6481 ggtacctcta caacaacaga agaccccagg tacgatacac ggtcgcttcc ggcgcggtcg
     6541 ccttcaaggt cgcgatagaa gagccgcgag gcgtggcaga cgagtacgtc tccgtctcgg
     6601 gggatcatct caaggaaaag gcgatgcccg ccgtcgccgc cgccttcgag tacaaggacg
     6661 gcggcatctc cgtctccccc agcgccttct atcagcagtt caggaacaag gccaacgacg
     6721 acaaccccga taccaaggac atcaccgtgg ccacttacgg cctgtgcttg agcggcagtg
     6781 tgaaggcgga ccccgcgacg ttcacgggag gcctctggta tggacaaaac ctttctgtct
     6841 tcgatctcga caagagaaag aacagcccat ccacggtgtt cggcaagccg gttgccgatg
     6901 aggcgggaaa cgacatagag gatatcgaca gcctgggcgg ctgggcccag gtatccctca
     6961 aggccggtcc cggggtgttc agggcggggg cgggcatgca gcgttctgaa accggctgga
     7021 agggggacgc ctacgaggac gacatctcta ccatgggcgc cttcgtcaat tacgagtaca
     7081 cggtggcgaa ggggttcacc gtcacgcccg agatcgccta cttcgactac ggcaaggatg
     7141 cgaacaagaa caaggtgtcg gaaggcaaga gcgacctcgg gagcgatacg cttgtaggtg
     7201 tccacctcca gtacgatttc tgacgcgatg gcgcaaggac cacggggcgc ttgggcgcac
     7261 cgtggtccct gcacaaacgg ggactggatg aaatctttct cgggttgtat acggaacagg
     7321 gtgtgattct tcgcggaggc tgcacgtgga taaggcaaga gtactgtttc tgtgcaccgg
     7381 gaactcggcc aggagccaga tggccgaggc gtttctcagg acatacgcgc ctcacgcttt
     7441 cgaggcgtac agcgcaggcc tcgagcccaa gggagtgcat cccttgaccg tcgaggtctt
     7501 ggacgagctc ggcatcgaca tccgttccca gcggtccaag cacctcgacg aatacatggg
     7561 caagatgttc ttcggctacc tcatcacggt ctgctcccag gcggatgcca gctgcccggc
     7621 ggtcttccct ggggtcggtg tgcgtatgca ctgggccttc gacgaccctg ctgcatgcag
     7681 gggcacacag gccgagtgcc tgcaggagtt ccgcagggtc cgcgacgaga tacgaaaccg
     7741 cgtactgtcc tggctcgagg agctccacgt cgaacccgcc cgcacatgaa cggccctggg
     7801 gagcctgagg gtgaacg
//
LOCUS       DAOSCR010000037         7753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6083_length_7753_cov_3.357366, whole genome shotgun sequence.
ACCESSION   DAOSCR010000037 DAOSCR010000000
VERSION     DAOSCR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_7753_cov_3.357366"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="folP"
                     /locus_tag="PLT69_01820"
     CDS             <1..580
                     /gene="folP"
                     /locus_tag="PLT69_01820"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOJ13220.1"
                     /translation="IVKEVDVPVSVDTSKHRVARAALEAGAVMVNDVTALRNAPAMMG
                     LVRSTGCGVVLMHMRGTPSTMQSDTSYGDVVAEVYAFLDERIEACLEAGIDPACIAAD
                     PGIGFGKDLAGNLALVRHISEFSSLGVPVLLGHSRKSFIGAVLDSAVDNRQDGTDAVT
                     AWAAANGVDIVRVHDCRRAKRTRAMIRAIAGA"
     gene            581..958
                     /gene="acpS"
                     /locus_tag="PLT69_01825"
     CDS             581..958
                     /gene="acpS"
                     /locus_tag="PLT69_01825"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOJ13221.1"
                     /translation="MIAGVGIDIVDIHRVASIVERYGERFLERILTPAELGRCRALLD
                     PAPYLAGRFAAKEAAFKALGAPRGSGIGFHDVSVDSSGGRPVITLGGRARERAAELDV
                     RDVHVSISHDGGCAVAVVILERP"
     gene            955..1404
                     /gene="tsaE"
                     /locus_tag="PLT69_01830"
     CDS             955..1404
                     /gene="tsaE"
                     /locus_tag="PLT69_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOJ13222.1"
                     /translation="MTTTFASSSPLDTLEIGARLGSLLKGGEIVLITGELGTGKTMLA
                     KGIAKGLDADPSTPVLSPSFTLVNIYHARLDIVHVDLYRLDSEEIVHLGLEDYLDSGH
                     VLVVEWADRAQGYFRGDLVEIAISHAGEDAREIRVSSDVPWLGPLAG"
     gene            1482..2714
                     /locus_tag="PLT69_01835"
     CDS             1482..2714
                     /locus_tag="PLT69_01835"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ13223.1"
                     /translation="MGIVVQKYGGTSVGSLEKIRFVANKVASRYREGHDVAVVVSAMA
                     GETDRLIRLAKDLSPTPDPREMDVLVSTGEQVSIALLAMALKDLGIPARSLLGFQIGI
                     KTDDAFMKARILDIDTQVLRKIFEAKEVAVVAGFQGITEDMEITTLGRGGSDTSAVAL
                     AAVLGADVCEIYTDVDGVYTCDPNIYDKARRLDKISYDEMLEMASLGAKVLQTRSVEF
                     AKKYNVPILVKSTFSDEGGTLTTQEDADMEKEVVSGITHDRNEAKITILGVQDKPGVA
                     AMIFTPLAENNINVDMIIQNVSADGKLADLSFTVSKSDYERALAIVKGIADRLGCRDV
                     VGDTSVAKLSIVGVGMRSHAGVASKMFSALSSVGINIQMISTSEIKISCVIDEKFIEL
                     GVRVLHDAFELDKSRGEA"
     gene            2711..4312
                     /gene="cimA"
                     /locus_tag="PLT69_01840"
     CDS             2711..4312
                     /gene="cimA"
                     /locus_tag="PLT69_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOJ13224.1"
                     /translation="MRRVEIYDTTLRDGAQAEDISLSLDDKVSIVSKLDQLGVHFIEA
                     GWPGANPKDDKLFARLRQMHPKNATILAFCSTCRPGQTPENDRLIKAVLNARPQGVTI
                     FGKTWDIHVSGQGGLGCTLEENVRMIRDTVAFLKTHYPMVIFDAEHFFDGFRANSEYA
                     LAAVKAASLGGADRVVLCDTNGGSLPDYIAATVGRVKATITSPIGIHCHNDSELAVAN
                     SLSAVQAGAVQVHGTINGVGERCGNANLCSIIPTLSLKLGCETIPRENLGMLSEISRF
                     FDDIANLSHDKHQPYVGEAAFAHKGGVHVSAVMGDPSTYEHIDPSLVGNQRKVLVSEH
                     SGKSNIIYKAREFNIDLSRDDATAAEIVKSIKDLEDCGFQFESADASLELLMKKAMGE
                     HMRFFKLKGFRVNTERRSDDTDPVSEATIMIEVDGNVEHTAALGNGPVNALDRALRKA
                     LSKFYPSIEEVQLLDYKVRVISSRKGTESVVRVLIESGDRKDHWSTVGVSSNILEASW
                     MALVDSMDYKLYKDSMKARARHKNR"
     gene            4716..5012
                     /locus_tag="PLT69_01845"
     CDS             4716..5012
                     /locus_tag="PLT69_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13225.1"
                     /translation="MKRTEQTYQENRKFEFQGMKKWLSRGEYIISTISEDGTIWTYSS
                     KVLPIFDFGSTKIFQAQESGVAEVSGVERGEEFYFSIQGVRVTKVYDYCYAEEL"
     gene            complement(5431..5655)
                     /locus_tag="PLT69_01850"
     CDS             complement(5431..5655)
                     /locus_tag="PLT69_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13226.1"
                     /translation="MIWALVYLPVAYYLWRVYASRIEPLSLSLMTAVVVGFCLVEWSM
                     LKRDGKRSRAMMAVLCLTLGSLLGGFFFRP"
     gene            5898..5970
                     /locus_tag="PLT69_01855"
     tRNA            5898..5970
                     /locus_tag="PLT69_01855"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5931..5933,aa:Lys,seq:ttt)
     gene            5980..6057
                     /locus_tag="PLT69_01860"
     tRNA            5980..6057
                     /locus_tag="PLT69_01860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6016..6018,aa:Glu,seq:ttc)
     gene            complement(6340..7299)
                     /locus_tag="PLT69_01865"
     CDS             complement(6340..7299)
                     /locus_tag="PLT69_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13227.1"
                     /translation="MAEEILFVDDESVILDIVRQVFSERGKDILTAAGPMEALDIVRS
                     NEVAVIVADNHMPGMTGIELLSRVKVISPDTVKIVMTGFADLETALEAINRAEVYRFV
                     VKPFENEQLASLVEDALERYRLVKSIMRGDQSTMRSLVHALELKDPYTRGHSERVAEY
                     AVLIARALGLAEDEQDAIRYGSWLHDCGKIGVSENILNYEGPLDEAEVHVIHNHPLWG
                     ADVARTGHLSRPIVNIILSHHERYDGKGYPLGLSGKDIPLEARIVSVADVYDALTTER
                     PYRGAYSPERALEMLDSMKGTILDPEIVGCFAQAMKEKIQGGS"
BASE COUNT         1507 a         2530 c         2456 g         1260 t
ORIGIN      
        1 catcgtgaaa gaggtggacg tgcccgtcag cgtcgacacc tccaagcacc gggtggccag
       61 ggcggccctc gaggcggggg cggtcatggt caacgacgtg acggccctga ggaacgcccc
      121 ggccatgatg gggctggtgc gctccaccgg gtgcggcgtc gtgctcatgc acatgcgcgg
      181 gacaccttcg accatgcagt ccgacacctc gtacggggac gtcgtggccg aggtgtacgc
      241 cttcctcgac gagaggatag aggcgtgcct tgaggccggg atagacccgg cctgcatagc
      301 cgccgacccc ggcatcgggt tcggcaagga ccttgcgggc aacctcgccc tcgtgcgcca
      361 catctcggag ttctcgtcgc tcggcgtacc cgtgctcctc ggccactcgc gcaagtcttt
      421 cataggggcc gtgctcgact ccgccgtgga caaccggcag gacggcaccg acgcggtgac
      481 cgcgtgggcg gctgcgaacg gcgtggacat cgtgcgggtg cacgattgca ggcgcgcgaa
      541 aaggaccagg gccatgatca gggccatcgc gggggcatga gtgatcgccg gggtaggcat
      601 agacatcgtc gacatccatc gcgtagcctc catcgtggag cgctacggcg agaggttcct
      661 cgagcgcata ctcaccccgg ccgagctcgg gcgctgccgg gccctgctgg acccggcccc
      721 gtacctcgcc gggaggttcg cggccaagga ggccgcgttc aaggccctgg gcgcccccag
      781 aggctcgggc ataggcttcc acgacgtgag cgtggactcc tctggcggca ggccggtgat
      841 aacgctcggc ggcagggcga gggagagggc ggcggaactg gacgtgcgcg acgttcacgt
      901 gagcatctcc cacgacggcg gctgcgcggt cgccgtcgtc atcctggaga ggccatgacg
      961 acgacgtttg cgagctcctc gccgctcgac accctggaga tcggcgcccg cctgggctcg
     1021 ctgctcaagg gcggcgagat cgtcctcatc acgggggaac tcggaacggg caagacgatg
     1081 ctcgccaagg gtatcgccaa ggggctggat gcagacccct cgaccccggt gttgagcccc
     1141 agtttcaccc tcgtgaacat ctaccacgcg aggcttgaca tcgtgcacgt cgacctctat
     1201 aggctagact ctgaagaaat cgtccacctc gggttggaag actacctgga ttccgggcac
     1261 gtcctggtcg tcgagtgggc cgacagggca cagggttact tcaggggcga tctcgtggag
     1321 atagccatct cgcatgcagg cgaggacgcc cgcgagatcc gcgtcagctc tgatgtccca
     1381 tggctggggc cgctggcagg gtgactcccg caggatcccg ccccgggggg aattcttcgt
     1441 tggaacacgt tcaagaacac cacgagacgg ggtgaaaggc gatgggtatc gttgtccaga
     1501 agtacggggg aacctcggtg gggtccctgg agaagatcag gttcgtggcg aacaaggtgg
     1561 cctcgcgcta ccgtgagggt cacgacgtgg ccgtggtggt ctcggccatg gcgggcgaga
     1621 cggacaggct catcaggctc gcgaaggacc tctcgcctac ccctgacccg agggagatgg
     1681 acgttctcgt gtcgacgggg gagcaggtga gcatcgcgct cctcgccatg gcgctcaagg
     1741 acctgggcat ccccgccagg tcgctcctgg gcttccagat cggcatcaag accgacgacg
     1801 ccttcatgaa ggcgaggatc ctcgacatcg acacccaggt cttgaggaaa atcttcgagg
     1861 caaaggaggt ggcggtggtc gccggtttcc aggggatcac cgaggacatg gagatcacca
     1921 cgctcggccg cggcgggtcc gacacctcgg cggtggccct tgccgccgtc ctgggcgcgg
     1981 acgtctgcga gatctacacc gatgtcgacg gcgtgtacac ctgcgacccg aacatctacg
     2041 acaaggcgag gaggttggac aagatctcgt acgacgagat gctcgagatg gcgtcactgg
     2101 gggcaaaggt tctccagacg cgctcggtgg agttcgcgaa gaagtataac gtgcccatcc
     2161 tcgtgaagag caccttcagc gacgagggag gcactctgac cacccaggag gatgccgaca
     2221 tggaaaaaga ggtcgtctca gggatcaccc acgacaggaa cgaggcgaag ataacgatct
     2281 tgggcgtaca ggacaagccc ggggtggcgg ccatgatctt tacgcccctg gccgagaaca
     2341 acatcaacgt ggacatgatc atccagaacg tctccgcaga cggcaagctc gcggacctga
     2401 gcttcaccgt gagcaagagc gactacgaaa gggccctcgc catcgtcaag gggatagcgg
     2461 acagactcgg gtgcagggac gtcgtgggcg acacatccgt cgcgaagctc tccatcgtcg
     2521 gcgtcggcat gcgctcccac gcgggggtcg cgtccaagat gttctccgcg ctctccagcg
     2581 ttggcatcaa catccagatg atctccacct cggagatcaa gatctcctgc gtgatcgacg
     2641 aaaagttcat agagctcggg gtaagggtgc tccacgacgc cttcgagctg gacaaatcga
     2701 ggggagaggc ttgagaaggg tcgagatcta cgacaccacc ctgcgagacg gcgcacaggc
     2761 cgaggacatc agcctgtccc tggacgacaa ggtatcgatc gtctccaagc tcgatcagct
     2821 cggcgtccac ttcatcgagg cggggtggcc cggcgcgaac ccgaaagacg acaagctgtt
     2881 cgcaaggctc aggcagatgc accccaagaa cgccacgatc ctggcgttct gcagcacctg
     2941 caggccgggc cagacacccg agaacgaccg cctcatcaag gcggtcttga acgcgaggcc
     3001 ccaaggggtg accatcttcg gcaagacgtg ggacatccac gtgagcggac agggcgggct
     3061 gggttgcacc ctcgaggaaa acgtgaggat gatcagggac acggtcgcct tcctcaagac
     3121 ccattacccc atggtgatct tcgacgcgga acacttcttc gacggcttca gggccaacag
     3181 cgaatatgcc ctcgcggcgg tgaaggccgc atctctgggc ggtgcggacc gggtcgtcct
     3241 gtgcgacacc aacggcggat ctctgcctga ctacatcgcg gctaccgtgg gcagggtcaa
     3301 ggcgaccatc acgtcgccca tagggatcca ctgccacaac gactcggagc tcgccgtggc
     3361 caattccttg agcgccgttc aggcaggcgc agtccaggtc cacggcacga tcaacggcgt
     3421 gggcgagagg tgcgggaacg cgaacctgtg ctccatcatc cccaccctct ccctcaagct
     3481 cggctgcgag acgatcccca gggagaacct cggcatgctg agcgagatct cgaggttctt
     3541 cgacgacatc gccaacctct cccacgacaa gcaccagccc tacgtcggag aggccgcctt
     3601 cgcccacaag ggcggggtcc acgtgagcgc ggtcatgggc gacccgtcca cctacgagca
     3661 catcgacccc tcactcgtgg gcaaccagcg caaggtcctc gtgtccgagc actcgggcaa
     3721 gagcaacatc atctacaagg cccgggagtt caacatagac ctctcgaggg acgacgccac
     3781 cgccgcagag atcgtcaaga gcatcaagga cctggaagac tgcgggttcc agttcgagag
     3841 cgcggacgca tccctggaac tcctcatgaa gaaggccatg ggcgagcaca tgcggttctt
     3901 caagctgaag ggcttcaggg tcaacacgga acgccgcagc gacgacaccg acccggtctc
     3961 cgaggcgacg atcatgatcg aggttgacgg caacgtcgag cacaccgcgg ccttgggaaa
     4021 cggcccggtc aacgcgctgg acagggcgct caggaaggcg ctctcgaagt tctacccgtc
     4081 catagaagag gtccagctcc tggactacaa ggtccgcgtc atctcgtcgc gaaaaggcac
     4141 cgagtctgtg gtcagggtcc tcatagagtc gggcgacagg aaggatcact ggtccacggt
     4201 gggcgtgtcc agcaacatcc tcgaggcctc ctggatggcc ctggtggaca gcatggatta
     4261 caagctctac aaggactcca tgaaggcacg ggcgcgccac aagaaccgct gagatctgcg
     4321 cccgcacgcc gcataccccg ctgcttccct ccatcgaccc ctcgcctccc ggcaaggagc
     4381 aaagcatgga gttacgcccg aggggaccac gcgcagagca ggcttgtggg ccgcaagcca
     4441 tgtgagggga ctcaccctgc tgggtgcccc accgcgcatg accctgccct tcgtctccag
     4501 ccggggcgtc ctgccgacgc ttgatgcctc tcatcggccg tcccctgccg cgcgccttcc
     4561 tggggcctct ggggcccggg ccggggacga cgaccctctc caggcaaggg gaaggaagcc
     4621 ccgggatgga cacgcccgcc cggctcacgt gtcaaggccc ccacgactcc ggccgataag
     4681 gtatgtaggg agggaaacca aggaaggtgc cagtcatgaa gagaaccgag caaacatacc
     4741 aggaaaacag gaagttcgag ttccagggga tgaagaagtg gctgagtcgg ggagagtaca
     4801 tcatctcgac catcagcgaa gacggcacca tatggaccta ctcgagcaag gtccttccca
     4861 tattcgactt cgggagcacc aagatcttcc aggcgcaaga gtcgggggtg gccgaggtga
     4921 gcggcgtgga gaggggagag gagttctact tctccatcca gggtgtccgg gtcaccaagg
     4981 tctacgacta ctgttacgcc gaggagctct aggcacaggg aagagcgacc gcccgaagac
     5041 gtccaccttc tcccacgccg accccagcag ggatctgcaa ggccctttgc ggcacacgat
     5101 gtcctgatgc acccctctcg ccacgtgcgg cttccgcccg cccggaaggc aggacaggac
     5161 cgcgaaggca tcgtccccca aacagggctg caaggacgaa ggtctcaggc atcgaacgag
     5221 ctttgcgccc aaggaggcgc accagttggg gcatcggggc gtcaacgggc acggcagccc
     5281 gggtgtcgtg cgaggggacc tgccccgctc tttaccggga agacctgcat gagcgagacg
     5341 cccacgcgca cgccgcaccc gtggcgggat cttcgtgagc agggccccag gcatctcatc
     5401 gcgctcaccc ggggcatatc cccgtagatc ctacggcctg aagaaaaacc cgcccaggag
     5461 cgatcccagc gtgagacaca gaacggccat catggcgcgc gaccttttcc cgtccctctt
     5521 cagcatggac cactcgacca ggcagaaacc cacgaccacg gcggtcatga gggagaggga
     5581 gagaggctcg atacgagagg cgtaaaccct ccacaggtag tacgcgacag gtaggtacac
     5641 aagcgcccag atcatgacga ttcgacacta caccacaagg gcctcaccgg gcaatcccct
     5701 gcatgacgcc agggtacgtc tcgatccccc gcaggcacgt tgttcactca cctgctgagc
     5761 atgcggacgg aagccttccc ttccccccgt cgagaccaag ccttgccgcg ggccacccac
     5821 tctgtcccca agcacctatc ctgctcatct tcttcttgat cttttcaccc atccatgaca
     5881 tagggggttg tcgaactgag ccagtagctc agtcggtaga gcatcggact tttaatccgg
     5941 tggtcgaggg ttcgagtccc tcctggctca ctggtaatcg tccccatcgt ctagccaggc
     6001 ccaggacacc ggcctttcac gccggcgaca ggggttcaaa tccccttggg gacgccactg
     6061 tataccccac aggggggaca tcggtcgatg accccctgta tctttccaac aggccaacag
     6121 gacaccaccg aggattcccg ggcaaggcac acgctcatgc atactgcgtc tcgtccgccg
     6181 gctgcacggt ggccacaggc caggctttca aggggaggct tcggggtgta tggatggaga
     6241 ggagccccgc cccaaggcac agtggagaca tgccaggctt tcatggggag ggctgcctgt
     6301 ctccggggcg caaggcgccg atcgcccggg tgcggcggat cagctcccac cctggatctt
     6361 ttccttcatg gcctgagcga aacacccgac gatctcgggg tcgaggatag tgcccttcat
     6421 ggagtcgagc atctcgagcg cccgctcggg ggaatacgcc cccctgtagg gccgctcggt
     6481 ggtgagcgcg tcgtagacgt cggccacgct cacgatccgc gcctcgagag gtatatcctt
     6541 gcccgagagc cccaaggggt accccttccc gtcgtagcgc tcgtggtgcg acaggatgat
     6601 gttcacgatg ggcctcgaga ggtggcctgt ccgggccacg tccgcgcccc agaggggatg
     6661 gttgtggatg acgtggactt ccgcctcgtc gagtgggccc tcgtagttga gtatgttctc
     6721 ggacacgcct atcttgccgc agtcgtgcaa ccagctcccg tagcggatcg cgtcctgctc
     6781 gtcctcggcc aggcccaatg cccgcgcgat caagaccgca tactcggcga cccgctccga
     6841 gtgccccctg gtgtaggggt ccttcagctc caatgcgtgg acgagcgagc gcatggtgga
     6901 ctggtccccc ctcatgatgg acttcacgag ccggtaccgc tcgagggcgt cctccaccag
     6961 ggaagcgagc tgctcgttct cgaagggctt cacgacgaac cggtagacct ccgcgcggtt
     7021 gatggcctcc agggccgtct ccagatcggc gaaccctgtc atgacgatct tcaccgtgtc
     7081 cggcgagatg accttgacgc gcgagaggag ctcgatcccg gtcatgcccg gcatgtggtt
     7141 gtcagccacg atcaccgcga cctcgttgga cctcacgatg tcgagcgcct ccatcggccc
     7201 ggccgcagtg agtatgtcct tgccgcgctc ggagaagacc tgccggacga tgtccaggat
     7261 taccgattcg tcgtcaacga agaggatctc ttcagccatt tccctacctc atcgtcctga
     7321 tgggtatcca agtttacgcc cagtcccact ccttggcaag gccggcgcct tttcgtgggt
     7381 ggagagcgcc tctgggctgt ccttccggga cagccgccac gggccgggcc tccatccttg
     7441 catcacctgt cccgcccgtc acgccgccga tcccgcccgt tatggaaagg gcccttgctc
     7501 acaccggctg ccgttctccc cacgcgagac ggcatgtcgg gcctgccgcc tgcgcgcgta
     7561 tcgagaaacc agggggcgtc tccccctgct ttcggcagcc tctcgagctt cgagcacagg
     7621 ggccgctccc ccatccggcg cgccgatgca tgccacccga gtcacaacct gtcgaacaac
     7681 ccggcgagga ccaccgcgcc gccctgccag ggacatccac ctgcgtaccc ctcctcgcgg
     7741 caggcacccg gcg
//
LOCUS       DAOSCR010000038         7683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6143_length_7683_cov_3.689827, whole genome shotgun sequence.
ACCESSION   DAOSCR010000038 DAOSCR010000000
VERSION     DAOSCR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6143_length_7683_cov_3.689827"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(860..2737)
                     /locus_tag="PLT69_01870"
     CDS             complement(860..2737)
                     /locus_tag="PLT69_01870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ13228.1"
                     /translation="MIFDWNETYRKRLTDARTALSRIRRGARIFIGSACGEPQLLVKT
                     LIEVASDLADTEVIHFLDLGIEPYTDEKFTGSFRHNALFIGASSRAAIREGRADYTPV
                     FLSEIPLLMQRGSMPVDVALITASPPDTNGYVSLGISVDITKTAAEVADYVVAEVNPN
                     MPRTLGDSFLHVSEIDAFVENDVPLLEFVQKPSGDIAKAIGEQIADLIENEATIQTGV
                     GRIPSAMMHYLAGKRDLGIHTETFTDDIIDLVEAGVVTCRKKTLHPGKIVASFCMGTR
                     RLYDYVNNNPLFEFRPCQYVNDPYVIAQNDKMVSINSALTIDITGQVCSDSIGFTFYS
                     GIGGQVDFVRGASLSRRGKSIMVLPSTTDDGKKSRIVAFNEPGSGIVVTRGSIHYVVT
                     EFGVAYVHGKSIRDRAMALISIAHPKFRDELLEAAKEQGYVYKDQILPVVLYPKEYET
                     YWTDKKGTEIFFRPVKPTDERAIQDMIYALPDQDIQTRFFHSLKSFPHKVAMPLAAID
                     YNDRMAIAAVIGKEEPESREQIVAVGRYIRDPRSNLAEVAFTTHQDWQNRGIGTFLLR
                     YLMRIAREKNIKGFTADVLARNTPMMKVFAKTGCPMKTRLEYGVYELEIEFGEVEQGK
                     G"
     gene            3538..4053
                     /locus_tag="PLT69_01875"
     CDS             3538..4053
                     /locus_tag="PLT69_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOJ13229.1"
                     /translation="MEERRRESLDRAFELGKNYEMANGGCPQCTLAAVFEVLGIEDEA
                     VFKAATALADGVGLTGDGHCGALSGGTLAIGYLYGREKKDFADIMRLVEANLLAKKLR
                     DRFVERYGTVRCRDLQQKFFGRFYNLYEPAELEAAFKAGMLNTCSDVVGEVARMTVEI
                     ILEKRASDTEG"
     gene            complement(4072..4620)
                     /gene="pgsA"
                     /locus_tag="PLT69_01880"
     CDS             complement(4072..4620)
                     /gene="pgsA"
                     /locus_tag="PLT69_01880"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOJ13230.1"
                     /translation="MNISNALTVSRIAMVPVIALALVRDDPILSFFAAAVFSVAMVTD
                     YLDGFFARKLHIESDLGKLLDPLADKFLIVTVLIMLIQLGRVPAWMVAVIVGRELAVT
                     GLRSIAAEKHIVIPADWLGKYKTAFQCVALIPLLVHHPIPLPVLSRLQFQEAGEFFLW
                     VAMFFSIWSGVEYFVGYARAGS"
     gene            complement(4617..5228)
                     /locus_tag="PLT69_01885"
     CDS             complement(4617..5228)
                     /locus_tag="PLT69_01885"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ13231.1"
                     /translation="MQRLKVFLVCMVAAATLYPGAAEADIYMRRGPDGTLHFSNCPMG
                     TDWKLYARENKKQYRYASYPSRTRNPYPPAGYEELITSAARTYGLNPRIIRSVIEVES
                     GFNPFARSPKGAMGLMQIMPQTAYDLGLNDPWDPAENIAAGTRYLSWLLRKYNGNLQL
                     ALAAYNAGPSAVDAYGGIPPYQETTEYVKNVLARIFEGGSSFQ"
     gene            complement(5298..6797)
                     /locus_tag="PLT69_01890"
     CDS             complement(5298..6797)
                     /locus_tag="PLT69_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13232.1"
                     /translation="MEGQTTRKWKILFADDNRELNDAMRQIFGEHGFDVVQAFDGDEA
                     FEVYCSERPDLVFLDYLMPKMDGVDVLEEIMAVDPHALVVFITGEGSEDTAVQAMKAG
                     AKDYVTKPFSIQYLIHIARKFIREHELVMENARLREKAESYRDSLVTITETMGEAVIT
                     IDAQGRIQFMNLMAKKLWGPSEEMQGKPVDVLVPDPNVNLYAEIKESTGAQEGHYQNE
                     YTFRKADGGIFLGLLSASSLKGAGGMGDIVLVIRDLTDIEMMRRQVINAEKLASLGKV
                     VEGVAHEIRNSLTSLGGFSRRLSRSVDENSAEKVYVDYIIEDVKRLESMILDIEEYVN
                     YTKIHRPNYAPTHIEDVIENALIKTFGSGKFSQVTYTVNIAPGIEDIYADHDFLVEAF
                     WHLFVNACESMDGKGTLKVDVTSHPNYVIVDVIDTGKGIPSSEIKEIFNPFYTSKARG
                     AGLGLSKVYMIIEEHGGFITVNSVVNEGTRMRVFLSKRRGHGPVIPKGA"
     gene            complement(6810..7295)
                     /gene="folK"
                     /locus_tag="PLT69_01895"
     CDS             complement(6810..7295)
                     /gene="folK"
                     /locus_tag="PLT69_01895"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOJ13233.1"
                     /translation="MIRGYLGIGSNEGDRALHLALAREELQARGVTIGRVSRVYETVP
                     VEVPGPQRPYYNMVAEFTFRGGPLGLLDVCLEVERALGRRRPYKNAPRTVDIDILFIE
                     GRRIKGPRLEVPHPRMEKRAFVVHPLAEIAPGFILPSGKKAEEVRDELGGADIVRVIG
                     A"
     gene            complement(7292..>7683)
                     /locus_tag="PLT69_01900"
     CDS             complement(7292..>7683)
                     /locus_tag="PLT69_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ13234.1"
                     /translation="RSSAPEYILGYTCFNDVTARDLQAKDGQWTRAKSFDTFAPIGPW
                     VDLSVGDPHGLAITASLNGRVVQESNTKNLVFDCFELVAFVSSVMTLMPFDVIATGTP
                     SGIGPMNKGDEICVTVEHIGTLRNHVE"
BASE COUNT         1335 a         2418 c         2424 g         1506 t
ORIGIN      
        1 aaggtacctc tcctgacggc catgtccctg ctcgtggggg gaatgctgct ctcgagcgcc
       61 gcatcaggcg tgtgctcgct ctacacggag ctcggcatca gcaccgtgaa ggaagagcgc
      121 aaaaaggtct gaggccgcaa gtaggaatcc tcacggctct tcgagcctcc ccctcgaggt
      181 gcagtctcga ctgcaagccc cctcgagggg accgtacccg gcaggggccc ttccgtgttt
      241 cccaggatcg gccgcagccg cgtcgccgct cgaacaaggc tcaacaaaag cgggcttgca
      301 ggtgacaccc tcttcgggtc gaacgggcgc cgtgggcacg gcctcgatcc ttccggcccc
      361 gatcgtcttc tccggctcgc atcagccccg gtgaacctgt cagcggcagc tcttggcccc
      421 tctcgagagg gccgcccatg ccgagggctg tcgttctttc tcgcaatcgt tccgagccgg
      481 tggtcgtgtc actcttgaga gagggccgca catgacaagg gccgagcgtg aggaaaggcc
      541 cacatacgag agcaccccac gccgggtgag atcggaaaga ccatggccgc ccacaggaag
      601 aactcccccg cctcccggga ccggtgcatc gagagaacag gaagcggcat gaggtgagcc
      661 gggccggggt cctgcgcgcc gcaggcatca cgccccactc tggggcgcct cgtgtccgaa
      721 cgcctcgcga acggaccggg cacgacatgg caggaccgcc ggtgccgtca cccgcgacgt
      781 gtcggacggg cgcacaggat ctcccgatgg gttccgcgga tcgggcaggg gtctttctcc
      841 gagaagggcc gatgcgccct cacccctttc cttgttccac ctcgccgaac tcgatctcga
      901 gctcataaac gccgtattcc aggcgggttt tcatggggca gccggtcttg gcaaagacct
      961 tcatcatggg cgtgttcctg gccaggacat cggccgtgaa ccccttgatg ttcttctccc
     1021 ttgcgatgcg catcaggtac cggagaagaa acgtgccgat acctcggttc tgccaatcct
     1081 ggtgcgtggt gaaggcgact tccgcgagat tggagcgggg gtctcggata tacctgccca
     1141 cggccacgat ctgctccctg ctctcaggtt cctccttgcc gatcaccgct gcgatggcca
     1201 tcctgtcgtt gtagtcgatg gccgcaaggg gcatggcgac cttgtggggg aaggacttga
     1261 gggaatggaa gaacctcgtc tggatatcct gatcgggcag ggcgtagatc atgtcctgga
     1321 tggcccgctc gtcggtgggc ttcaccggcc tgaagaagat ctccgtcccc ttcttgtcgg
     1381 tccagtaggt ttcgtattcc ttcgggtaca gaaccacggg caggatctgg tccttgtaga
     1441 cgtatccctg ttccttggcc gcctccagca gctcgtcgcg gaacttcggg tgggcgatgg
     1501 agatgagggc catcgcccgg tcgcggatgg acttgccgtg gacgtacgcc accccgaact
     1561 ccgtgaccac gtagtggatg gaccccctcg tcaccacgat cccgctgccc ggctcgttga
     1621 acgcgacgat ccgggatttc ttcccgtcgt ccgtggtgga cggcagcacc atgatcgact
     1681 tgcccctcct cgacagggaa gccccacgga cgaagtccac ctgccctccg atgccgctgt
     1741 agaacgtgaa cccgatggag tcggaacaga cctggccggt aatgtcgatc gtgagggccg
     1801 agttgatgga taccatcttg tcgttctgcg cgatgacgta ggggtcgttc acgtactggc
     1861 acggcctgaa ctcgaacagc ggattgttgt tcacgtagtc gtacaggcgc ctggtcccca
     1921 tgcagaaaga cgcgacgatc ttccctgggt gcagggtctt cttcctgcac gtgacgacgc
     1981 ccgcctcgac gaggtcgatg atgtcgtccg tgaaggtctc cgtgtggatg cccaagtccc
     2041 tcttgccggc gagatagtgc atcatcgcgc tcggtatcct gcccaccccg gtctggatgg
     2101 tggcctcgtt ctcgatgagg tcggctatct gctcccctat ggccttggcg atgtcgcccg
     2161 agggcttctg cacgaactcc aaaagcggaa cgtcgttctc cacgaacgcg tcgatctcgc
     2221 tgacgtgaag gaagctgtcg cccaaggtcc ggggcatgtt cgggttcacc tcggccacca
     2281 cgtagtccgc cacctcggcc gcggtcttgg tgatgtccac tgagatcccc aggctcacgt
     2341 aaccgttggt gtccgggggc gaggccgtga tgagggccac gtccaccggc atgctgccgc
     2401 gctgcatgag cagcgggatc tcggacagga agacgggcgt atagtccgcc ctgccttccc
     2461 tgatggccgc gcgggagctg gcgccgatga acagggcgtt gtgacggaag ctgccggtga
     2521 acttctcgtc cgtgtagggc tcgatcccga ggtcgaggaa gtggatgacc tcggtgtcgg
     2581 cgaggtctga ggcgacctct atgagggtct tgaccaggag ctggggttcg ccgcaggccg
     2641 acccgatgaa tatgcgcgcg ccccgcctga tcctggaaag ggcggtcctc gcgtcggtca
     2701 ggcgcttcct gtaggtctcg ttccagtcga agatcatgat cctttccctg cctgtcgcgt
     2761 ccgtgaaggg tggaggcgcg gtaccgtgcg gatgtctcgc ctccacctct gaaataacgg
     2821 cgatcgtgac agaagcaagg cctgtacccg gcgtgcaccg aggagcccgt ggcgaagcgg
     2881 gatgcaccga tcgggtcgtc ggcgcatccc gcggcaacga ggcccgcccg ggtaaatcac
     2941 ggcccaaagg ggccggggaa gtgcccggct cgctccttgc ccggcgcctc ccggtgctgt
     3001 ggcgacggga ggggattcgg tctgtcacga ggacacaggc ggcgaaggcg ggcaggacgc
     3061 ctggagggtg gggcgcttcc tgtaccccct ctctgggaca agaccgcggg ccatgctgta
     3121 cgggtaggac aaagcatggt gcgcgtgccc ttgtggggcc aggttcggcc gtcgtgggcc
     3181 cgggtgtatc ctgcgcgcgc caagtccgtg catccccgcc gtcacggctg aagggacctt
     3241 ccccgcttgt ccgagggtgt ccgcacggca cctctgccca aggacgtacg ccctggcttc
     3301 gtgcaatccc ggaggagaga ccgccgcttg cttccccatg gaaaccggtg ccgaagcccg
     3361 gtcttcatgg cctatctttc ctgtggtaca gttgtgcaag ggggtggctg ggtcgagggg
     3421 gatgacgcgc ccggcctgtg gcggggcccg tcacggcgcg gcgggatccc tgtcttcgcg
     3481 ggagacgctg ggagaaggat tgcgccttca cggtgcgagc ggcaaaggga gggggccatg
     3541 gaagagagac gtcgagagag tctggacagg gcattcgagc tggggaagaa ctacgagatg
     3601 gcgaacgggg gctgcccaca gtgcacgctc gcggccgtgt tcgaggtgct ggggatcgag
     3661 gacgaggcgg tgttcaaggc ggccactgcc cttgcggacg gcgtcgggct caccggcgac
     3721 ggccattgcg gtgcgctctc gggcggcacc ctcgcgatcg gctatctcta cggaagggaa
     3781 aagaaggatt ttgcggacat catgagactc gtggaggcca acctcttggc caagaagctg
     3841 cgcgaccggt tcgtggagag atacggcacg gtgaggtgcc gcgatctcca gcagaagttc
     3901 ttcggcaggt tctacaacct ctacgagccg gcggaactcg aggccgcctt caaggccggg
     3961 atgctgaaca cgtgctcgga cgtggtcggg gaggtcgccc gcatgaccgt cgagatcatc
     4021 ctcgagaaga gagcgtccga caccgagggc tgagcgcgga gacaggcagg cctacgagcc
     4081 tgctcgcgcg tacccgacga agtactccac gccggaccag atcgagaaga acatggccac
     4141 ccacaggaag aactcccccg cctcctggaa ctggagcctc gagagcacag gcagggggat
     4201 ggggtgatgc acgagcaggg ggatgagggc gacgcactgg aaggcggtct tgtacttgcc
     4261 cagccagtcc gccgggatga cgatgtgctt ctccgcggcg atgctcctga gcccggtcac
     4321 cgcgagctca cggcccacga tcaccgcgac catccacgca ggcactctcc ccagctggat
     4381 gagcatgatg aggaccgtga cgatcaggaa cttgtctgca agggggtcga ggagcttgcc
     4441 gagatccgac tctatgtgga gcttcctggc gaaaaagccg tcgaggtagt cggtgaccat
     4501 ggccacggag aagacggcgg ccgcgaagaa cgacaggatg ggatcgtcac gtacaagggc
     4561 cagcgcaatg accgggacca tcgcgatcct ggacacggtc aaggcattcg atatgttcat
     4621 tgaaaggaac ttcccccctc gaagattctc gcgaggacgt tcttaacata ctccgtggtt
     4681 tcctggtagg gaggaattcc gccgtatgcg tccacggccg acggacccgc gttgtacgcc
     4741 gcgagagcga gctggaggtt cccgttgtac ttcctcagca gccacgaaag gtacctcgtg
     4801 cccgcggcta tgttctccgc cgggtcccag gggtcgttca agccgaggtc gtaggccgtc
     4861 tgcggcatga tctgcatgag acccatcgcg cccttgggcg atcgagcaaa gggattgaac
     4921 cccgactcca cctcgatgac gctcctgatg atcctcgggt tgagcccgta ggtccttgca
     4981 gcgctggtga tgagctcctc gtacccggcg ggcgggtacg ggttcctcgt tcgggaaggg
     5041 taggaggcgt accggtactg cttcttgttc tcccgggcgt agagcttcca atccgtcccc
     5101 atggggcagt tgctgaagtg aagggtcccg tccgggcccc gcctcatgta aatgtccgcc
     5161 tcggcggccc ctggatacaa cgtcgcggct gcaaccatgc acaccaagaa aaccttaagc
     5221 ctctgcatac catggtatat cgtcatcagg gggcattttt ttgaatattt caatatcgta
     5281 cccatacccg agtcccctca ggcccccttg gggatcacgg gcccgtgtcc ccggcgcttc
     5341 gagaggaaca ccctcatcct cgtgccctcg ttgacgacgc tgttcaccgt tatgaacccg
     5401 ccgtgttcct cgatgatcat gtacaccttc gacagcccga gcccggcgcc ccttgccttg
     5461 ctcgtgtaga acgggttgaa gatctccttg atctcgctgc tgggtatccc cttgccggtg
     5521 tcgatgacgt caacgatcac gtagttggga tgcgaggtca cgtccacctt cagcgtcccc
     5581 ttcccgtcca tcgactcgca cgcgttcacg aagagatgcc agaaggcctc caccaggaag
     5641 tcgtggtcgg cgtaaatgtc ctcaatgccg ggggctatgt tcacggtgta ggtcacctgg
     5701 ctgaacttcc cggaaccgaa ggtctttatg agtgcgttct cgatcacgtc ctcaatgtgt
     5761 gtgggcgcgt agttcggacg gtggatcttc gtgtagttga cgtattcctc gatgtccagg
     5821 atcatggatt ccagcctctt gacgtcctcg atgatgtagt ccacgtagac cttctcggcc
     5881 gagttctcgt ccacggaacg gctcaggcgg cgtgaaaacc ctcccaggga cgtcagggag
     5941 ttcctgattt cgtgcgcgac gccttccacc accttgccca gagaagcgag cttctccgcg
     6001 ttgatcacct gcctgcgcat catctcgatg tcggtgagat cccttatcac gagcacgatg
     6061 tcccccatgc cgcccgcccc cttgagcgac gaggcgctca agagacccag gaagatcccg
     6121 ccgtcggcct tcctgaaggt gtactcgttc tggtagtggc cttcctgcgc gcccgtcgac
     6181 tccttgatct cggcatagag gttcacgttc gggtcaggca ccagcacgtc gacgggcttt
     6241 ccctgcattt cttcagaagg cccccagagc ttcttggcca tgaggttcat gaactggatc
     6301 ctaccctggg cgtcgatggt gatgaccgcc tcacccatgg tctccgtgat ggtgaccagg
     6361 ctgtcccggt aggattcggc cttctccctg agcctggcgt tctccatcac gagctcgtgt
     6421 tccctgatga acttcctggc gatgtggatc aggtactgaa tggagaacgg ctttgtcacg
     6481 taatccttcg ccccggcctt catggcctgg accgccgtgt cctcggaacc ctctcccgtg
     6541 atgaacacga cgagggcatg ggggtcaacg gccatgatct cttcgagcac gtccaccccg
     6601 tccatcttgg gcatgaggta atcgaggaac accaggtcgg gcctctccga acagtagact
     6661 tcgaacgcct cgtcaccgtc gaaggcctgc accacgtcga agccgtgctc gccgaagatc
     6721 tgccgcatgg cgtcgttcag ctcgcggttg tcgtccgcga acaggatctt ccactttctg
     6781 gtcgtttgac cttccatgtc tttcccggac taggcgccga tcacccggac aatgtccgcc
     6841 cccccgagct cgtccctcac ctcctcggcc ttcttcccac tgggaaggat aaagcccggt
     6901 gcgatctctg caaggggatg gacgacgaac gcccgctttt ccatcctcgg gtgagggacc
     6961 tcgaggcggg gccccttgat gcgcctgccc tctatgaaca ggatgtcgat gtccacggtc
     7021 ctcggcgcgt tcttgtaggg cctgcgcctt cccagggccc tctccacctc caggcacacg
     7081 tcgaggagtc ccagcggccc tccccgaaac gtgaattccg caaccatgtt gtagtatgga
     7141 cgctgggggc ccggcacctc aaccggcacg gtctcgtaca ccctcgagac gcggccgatg
     7201 gtcactcccc tggcctgaag ctcctctctc gcaagggcga gatgcagggc cctgtcgccc
     7261 tcgttggaac cgatgccgag gtatcctctt atcattccac gtggtttctc agcgtgccta
     7321 tgtgttcgac agtgacgcag atctcgtcgc ccttgttcat ggggccgatc cccgagggcg
     7381 tgccggtcgc gatcacgtcg aagggcatga gcgtcatgac ggacgagacg aacgccacga
     7441 gctcgaaaca gtcgaacacg aggttcttgg tgttcgactc ctgcacgacc ctcccgttca
     7501 ggctcgccgt gatggcgaga ccgtgcggat cccctaccga gagatccacc cacggcccga
     7561 tgggtgcgaa cgtgtcgaag gacttggcgc gtgtccactg gccgtccttt gcctggaggt
     7621 cgcgcgcggt gacgtcgttg aagcacgtgt acccgaggat gtactcgggt gcgctggatc
     7681 tgt
//
LOCUS       DAOSCR010000039         7490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6371_length_7490_cov_3.732885, whole genome shotgun sequence.
ACCESSION   DAOSCR010000039 DAOSCR010000000
VERSION     DAOSCR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6371_length_7490_cov_3.732885"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLT69_01905"
     CDS             <1..544
                     /locus_tag="PLT69_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ13235.1"
                     /translation="MASITARPASSCISTGHRPQPSSSARVATNQAATGRAAAMGARL
                     KEGIQEIASKNRDCVKEVRGLGLMIGIEYVHEFIGALMAECLARQGVWAVYSGNAPQV
                     MRFQVPITIGGHEVDDLLDRIRAAFDAMRWYLVLLMPLARIPFVGALLNNVHLQIWAF
                     NLVRDLEEFVGKIMNRRGGA"
     gene            546..1790
                     /locus_tag="PLT69_01910"
     CDS             546..1790
                     /locus_tag="PLT69_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ13236.1"
                     /translation="MKKADGSHNRARGLFDTAEKVFSRSYVNLLREKGHDVIEQEGDG
                     SWMTDAQGGRYLDCYASAGTYNLGRRNRAIARRFMKAVHETDQGNFVMPSDYKALLAQ
                     RIASFVPGRLDCVLFGVTRGESMDAACRLARGATSRPGIVTVDGGCYGETGFALSLTA
                     KAGKEQFGGLIPEVLAVPFGDEDAALRAVTDRTAAFVMEPIQAENHCRESSREYALRI
                     RRLCDATKTVLIFDETQTGFGRTGTRFFFEQLGVEPDILVIGEAVTSGMFPMTAMVFT
                     PELKRFFDIHPLIHLCTFGGHDLGCVVAMAALDEYELAEPWVNARMMGQRLASGLHAC
                     AQEFPDKAASVAGRGLLFSLRLAGGEPAAKAFCARAMREGLLVETGRVDTSTVLFRPS
                     LLVSEDEVDRIVQAVRTILAGL"
     gene            1906..2496
                     /locus_tag="PLT69_01915"
     CDS             1906..2496
                     /locus_tag="PLT69_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13237.1"
                     /translation="MARRKVQEKRRLQILKALDACLQRKSFEKTSIKDIAREAGVNHG
                     VLHYYFKSKEEILLNYIDYVIEDYRAQAREWMGGRDLEHSTKREFIDEVFAFVNNRIT
                     LNKSLSRIFVEIWEIALYNPAVRGKLREAYTRWVEDLGEMISRHIEDKAFARNVSIAM
                     VAFWEGMALFSTVFPPGSLAVGDVLKGFQQRIVEIL"
     gene            complement(2524..3216)
                     /gene="aqpZ"
                     /locus_tag="PLT69_01920"
     CDS             complement(2524..3216)
                     /gene="aqpZ"
                     /locus_tag="PLT69_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HOJ13238.1"
                     /translation="MTKRLLAEFFGTFWLVLGGCGSAVLSAAFPNVGIGLLGVALAFG
                     LTVLTMAFAVGHISGCHLNPAVTCGLVAGKRFPVKDLVPYVVSQVLGGIAGAFVLFII
                     ASGAAGFDVAAGFASNGYGEHSPGGYSLLAALVCEVVMTFMFLLVIMGATDERAPKGF
                     APIAIGLCLTLIHLISIPVTNTSVNPARSTGVAIFVGGWAVEQLWLFWVAPIAGGVLG
                     GIVYRVMGSPDN"
     gene            complement(3415..4587)
                     /gene="ahbC"
                     /locus_tag="PLT69_01925"
     CDS             complement(3415..4587)
                     /gene="ahbC"
                     /locus_tag="PLT69_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HOJ13239.1"
                     /translation="MIGISKLYCGTVEASDPLRYGRASRELPSHLLQFSADKKPVVVW
                     NVTSACNLRCVHCYASAGKAARELSTRQGLELIAQLADYGAPVILFSGGEPVLREDLP
                     TLIDAAVKAGMRAVISTNGTLISRDTAMRFADLGLSYIGVSLDGMEEVNDSFRGVQGA
                     FAQALGGIYNAMREDIKVGLRFTMNRRNVAEIPKIFDLMASEGIPRICFYHLVYTGRA
                     SELMEEDLTHGQTRQTLDLIMDRTKEMIQAGRDVEVLTVDNHADGPYVYLRLKREGSP
                     RAGEVLELLKMNAGNSSGLGIGCVNWDGEVYPDQFWRTRVLGNVTQRPFPEIWDDPSN
                     EFLMMLKNKKDHVQGRCRACRWLDVCAGNFRARAEAASGDPWAEDPACYLLDEEIA"
     gene            complement(4584..6086)
                     /gene="cobA"
                     /locus_tag="PLT69_01930"
     CDS             complement(4584..6086)
                     /gene="cobA"
                     /locus_tag="PLT69_01930"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOJ13240.1"
                     /translation="MPDKVSPAKVYLIGAGPGDPELVTLKATRIISGCDAVVYDDLIA
                     REILALAPQGARRIFVGKRGGRASMGQDEINELLVGLAREGLVTARIKGGDPCVFGRG
                     GEEALYLAERGVPFEFVPGVSSAIAAPECALIPPTHRGVAASVTLVTAHEDPSKEGGF
                     LDWAHLAGDPGTLVFLMGASRAASIADRLMKEGMDPETPCAMIQDATLPGQRTLVSTL
                     ASLGADAEREGMRSPSVIVVGRVVGLRSRLAVEAGLPLKGRSVLITRPAHLAGPTSAL
                     FASRGARALVYPLVEIKEMPFELPEPGSIDVFVFTSQNAVDLFFSRIRAEGRDARFFG
                     GARVYCIGPKTRDALARYGIAADGMAEEFVAEGLVELLKDMDLEGKRVCLPRAAGARP
                     VLAEALRHKGAVVEEIVVYETVMPKTASCEGFVEALREVDTVVFTSPSGVRHAAELLG
                     GGTDMLAAKTIVAIGPVTAQALRRLGMKPAVTAARYTDEGILEALTGEGS"
     gene            complement(6079..6966)
                     /gene="hemC"
                     /locus_tag="PLT69_01935"
     CDS             complement(6079..6966)
                     /gene="hemC"
                     /locus_tag="PLT69_01935"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOJ13241.1"
                     /translation="MRIGTRGSRLALAQTALVMDALGKKAPGVAFEVVRIKTTGDILS
                     DRPLDVIGGKGVFVKDIERALLDGSIDIAVHSLKDMQAHLPDGLVIGAYIEREDPRDM
                     VFSSSGLGLEELPEGSKVGTSSLRRKCQIRNARPDLQVVDIRGNVQTRLNRLGRTVDA
                     VVLAAAGVARLGLRPGVALDPGVMVPSPGQGVIAVECRVGDDETMGLLSSIDHAQTRT
                     CALAERAFLAELGQDCRLPAGALARLEGGSIAMVGMLGSADCRFCTRREMEGDGPRVG
                     AELARALLVDVKRRGSMDA"
     gene            complement(6944..>7490)
                     /locus_tag="PLT69_01940"
     CDS             complement(6944..>7490)
                     /locus_tag="PLT69_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOJ13242.1"
                     /translation="ELASSDVVITSTGAARPIITREMVQRAMGIRRNRPLIIVDIALP
                     RDVDEGAKECYNCYLYDIDALQAIVDRHFAEREAEAARAMAIIEEEVATFERWLRSLS
                     AQATIRDLYALADAIAVEQAREVSSKVDATVVEQALRTSLRRMLHRPVSFLKEHPDAR
                     HIEYVRRIFRLDEDHEDRHKG"
BASE COUNT         1272 a         2504 c         2465 g         1249 t
ORIGIN      
        1 gatggcctcg atcacggcgc ggcccgcttc gtcgtgcatc agcacgggcc acaggccgca
       61 gccgtccagc agcgcgcggg tcgccaccaa ccaggccgcc accgggcgcg cggcggccat
      121 gggcgcgcgt ctcaaggagg gcatccagga gatcgcctcg aagaaccgcg attgcgtgaa
      181 ggaggtcagg ggcctgggcc tcatgatcgg catcgagtac gtgcacgagt tcatcggcgc
      241 gctcatggcg gagtgcctgg ccaggcaggg tgtgtgggcg gtctactccg ggaacgcccc
      301 ccaggtcatg cgcttccagg tccccatcac gatcggcggc cacgaggtgg atgatcttct
      361 cgaccgcata cgcgcggcgt tcgacgccat gcggtggtac ctcgtcctgt tgatgccact
      421 ggccaggatc cccttcgtgg gggcgctcct gaacaatgtt catctccaga tctgggcgtt
      481 caacctggtc agggacctgg aggagttcgt cggaaagatc atgaaccgga ggggaggtgc
      541 gtgacatgaa gaaggcggac ggttcgcaca accgtgcacg agggctcttc gacacggcgg
      601 agaaggtgtt ttcccgaagc tacgtgaacc tgctcaggga gaaggggcac gacgtcatcg
      661 agcaggaggg ggacggttcg tggatgacgg acgcgcaggg cgggaggtat ctcgactgct
      721 acgcctccgc agggacgtac aacctgggca ggcgcaaccg ggcaatagcc cgtcggttca
      781 tgaaggcggt gcacgagacg gaccagggaa acttcgtcat gccctccgac tacaaggccc
      841 tcctggcgca gaggatcgcc tcgttcgtgc cgggcaggct cgactgcgtc ctgttcgggg
      901 tgaccagggg cgagtccatg gatgcggcct gcaggctcgc gcgcggagct acctcgaggc
      961 cggggatcgt cacggtcgac ggtggatgct acggtgagac gggtttcgcc ctgtccctga
     1021 cggccaaggc gggcaaggag caattcggcg gcctgatccc ggaggtcctc gccgtgccct
     1081 tcggggacga ggacgcggcc ttaagggcgg tcacggacag gaccgcggca ttcgtcatgg
     1141 aaccgatcca ggcggagaac cactgcaggg agtcgagccg tgaatacgca ctccggatac
     1201 ggcgcctgtg cgacgccacg aagacagtgc tcatcttcga cgagacgcaa accgggttcg
     1261 ggaggacagg cacaaggttc ttcttcgaac agctgggcgt ggagcctgac atcctcgtca
     1321 tcggcgaggc agtgacctcg ggcatgttcc ccatgaccgc catggtcttc acccctgagc
     1381 tcaagcgctt cttcgacata caccccctca tacacctgtg caccttcggg ggacacgatc
     1441 tgggctgcgt cgtggcgatg gccgcactcg acgagtacga gctggcagag ccttgggtca
     1501 acgcgcgcat gatgggtcag aggctcgcat ccggccttca tgcatgcgcg caggagttcc
     1561 cggacaaggc ggcctcggtc gccggaaggg ggctcctctt ctcgctcagg cttgcaggcg
     1621 gggaaccggc ggcgaaggcc ttctgcgccc gggcgatgcg ggagggcctc ctggtggaga
     1681 cgggaagggt ggacacctcg accgtcctct tcaggccgag cctgcttgtc tccgaagacg
     1741 aggtggacag gatcgtgcag gctgtgagaa ccatactggc cggtctctga aggaccggcc
     1801 ccacccgggg gaacccgcag gtgatcgtct cgatgaccgc ggcttgggtg catggccgga
     1861 cccggcgcgt atgagaccgg gacccgtcac ggaaaggaca tgaccatggc gcgcaggaag
     1921 gtgcaggaaa agaggaggct ccagatcctg aaggccctcg acgcctgcct gcagaggaag
     1981 tccttcgaga agacctccat caaggacatc gcccgcgagg cgggggtgaa ccacggggtg
     2041 ttgcactatt acttcaagag caaggaggag atcctcctga actacataga ctacgtcatc
     2101 gaagactacc gggcccaggc ccgggaatgg atgggcggcc gtgacctgga acactccacc
     2161 aagagggagt tcatcgacga ggtgttcgcc ttcgtcaaca accgcatcac cctgaacaag
     2221 tcgctctcgc ggatcttcgt ggagatctgg gagatcgccc tgtacaaccc cgcggtccgc
     2281 ggaaagctcc gtgaggccta cacccggtgg gtggaggatc tgggggagat gatctcccgg
     2341 cacatcgagg acaaggcatt tgccaggaac gtgagcatcg ccatggtggc cttctgggag
     2401 ggcatggccc tgttctccac cgtgttcccg ccggggtctc tggccgtcgg ggatgtcctc
     2461 aaggggttcc agcagcggat cgtcgagatc ctctgagcct ctcgtgccga tggggggccg
     2521 gggctagttg tccgggctcc ccatgacccg gtacacgatc ccgccgagca cgccgcctgc
     2581 gatgggcgcc acccagaaga gccagagctg ctccacggcc cacccgccca cgaagatcgc
     2641 gacaccggtg ctccgggccg ggttcaccga ggtgttcgtc accgggatgc tgatgaggtg
     2701 gatgagcgtc aggcagagcc cgatggcaat gggcgcgaac cccttgggcg cccgctcgtc
     2761 cgtggcgccc atgatgacca ggaggaacat gaaggtcatg accacctcgc agaccagggc
     2821 cgccaggagc gaataaccgc ccggcgaatg ttccccgtat ccgttgctgg caaagcccgc
     2881 ggccacgtcg aaccctgccg cgccggaggc tatgatgaag agcacgaagg cgccggcgat
     2941 gccccccagc acttgggaga ccacgtatgg gacgaggtct ttcaccggga acctcttgcc
     3001 tgccaccagt ccgcaggtga ccgccgggtt caggtggcag cccgagatgt gtcccacggc
     3061 aaatgccatg gtgagcacgg tgagcccgaa ggccagcgcc acgccgagga gcccgatgcc
     3121 cacgttggga aatgcggccg acaggaccgc gctgccgcat ccgccgagaa caagccagaa
     3181 cgtgccgaag aattccgcca ggagtctctt cgtcatggga ctacctcctt ggaatgagaa
     3241 tgaaaagggt gcggtttctt gcggggtgtg tgattttctg atgagagaat actcccaccc
     3301 ggcactctgt gcaaggacgg atcgtaacag aggggcacgc cgcgccgggc gcacgaaaga
     3361 acatgtccgg ctcttcgggt ccgccggggt cgtctccgcg aagagatgcc tgggttacgc
     3421 gatctcctcg tctaagaggt agcaggccgg gtcctcggcc caggggtcgc cggaggccgc
     3481 ctcggcccgg gcccggaagt tgccagcgca cacgtcgagc cacctgcagg ccctgcacct
     3541 gccctgcacg tgatccttct tgttcttgag catcatgagg aactcgttcg acgggtcgtc
     3601 ccagatctcg gggaagggcc tttgtgtgac gttgcccagc accctggtcc tccagaactg
     3661 gtcggggtac acctccccgt cccagttgac gcacccgatc cccaggcccg aggaattccc
     3721 cgcgttcatc ttcaggagct ccagcacctc gccggcccgt gggctgccct cccgcttcag
     3781 tctcaggtac acgtagggcc cgtccgcgtg gttgtccacg gtgaggacct ccacgtcgcg
     3841 gcctgcctgg atcatctcct tggtgcggtc catgatgagg tcgagggtct gccgggtctg
     3901 cccgtgggtg aggtcttctt ccatgagctc gctggccctc cccgtgtaca ccaggtggta
     3961 gaagcagatc cgggggatcc cctccgaggc catgaggtcg aagatcttcg ggatctcggc
     4021 gacgttgcgg cggttcatgg tgaagcgcag gcccaccttg atgtcctcgc gcatggcgtt
     4081 gtagatgccg ccgagagcct gggcgaaggc gccctgaacg ccccggaagc tgtcgttcac
     4141 ctcctccatg ccgtcgaggc tcacccctat gtacgaaagc ccgagatccg cgaacctcat
     4201 ggccgtatcc cgggaaatga gcgtgccgtt ggtggagatg accgcgcgca tgccggcctt
     4261 cacggcggca tcgatgagcg tgggaaggtc ttcgcggagc acgggctcgc cgcccgagaa
     4321 caaaatcacg ggggccccgt aatcggcaag ctgtgcaatg agctcgagac cctgtcgagt
     4381 cgagagctcc cgagccgcct tcccggcgga ggcatagcag tgcacgcagc gaaggttgca
     4441 ggcgctggtc acgttccaca ccaccaccgg cttcttgtcg gccgagaact gcagcaggtg
     4501 ggaaggcagc tccctggacg cacggccgta gcgcaggggg tcggatgcct ccacggtgcc
     4561 gcagtagagt ttcgagatgc cgatcatgac ccctcccccg taagagcctc gaggatcccc
     4621 tcgtcggtgt accgtgccgc cgtcaccgcc ggtttcatgc ccaggcggcg taaagcctgg
     4681 gccgtgaccg gaccgatcgc cacgatcgtc ttggcggcga gcatgtccgt gccgcctccc
     4741 aggagctctg ctgcgtggcg gacgccggaa gggctcgtga acaccacggt gtccacctcg
     4801 cgcaaggcct cgacgaaacc ctcgcaagac gcggtcttcg gcatgaccgt ctcgtagacc
     4861 acgatctcct cgacaacggc ccccttgtgt ctcagcgcct ctgcgagcac gggcctcgcc
     4921 cccgccgcgc gcggcaggca cacccgtttc ccctcgaggt ccatgtcctt gaggagctcc
     4981 accaggccct ctgcgacgaa ctcctcggcc atgccgtcgg cggcgatccc gtagcgcgcg
     5041 agggcgtctc tggtcttggg cccgatgcag taaacccgcg cccccccgaa aaagcgcgcg
     5101 tccctgcctt cggcccggat cctcgagaag aagaggtcca ccgcgttctg cgaggtgaag
     5161 acgaacacgt cgatcgagcc cggctccggg agctcgaagg gcatctcttt gatctccacg
     5221 agcgggtaga ccagggcgcg cgcccccctg gacgcgaaga gcgccgaggt ggggcctgcc
     5281 aggtgagcgg gcctcgtgat gaggacggac ctgcccttca gcgggaggcc agcctccacg
     5341 gcgagcctcg agcgcaggcc cacgacccgg cccaccacga tgacgctcgg cgatctcatg
     5401 ccctcgcgct ctgcgtcggc tcccagggag gcgagcgtgg agacgagggt cctctggccg
     5461 ggcagggtcg catcctggat catcgcgcac ggcgtctcgg ggtccatccc ttccttcatg
     5521 agccggtcag cgatcgatgc cgctcggctc gcccccatga ggaacacgag cgtgcccggg
     5581 tccccggcga ggtgggccca gtcgaggaaa ccgccctcct tggacgggtc ctcgtgcgcg
     5641 gtcacgagcg tcaccgatgc cgcgacgccc cggtgggtgg gggggatgag ggcgcactcc
     5701 ggggcggcga tcgcggagct cacgcccggg acgaactcga aggggacacc cctttcggcc
     5761 aggtagaggg cctcctctcc gcccctgccg aagacgcacg ggtcgccccc cttgatcctc
     5821 gccgtcacca ggccctcgcg cgcgagcccc acgaggagtt cgttgatctc gtcctggccc
     5881 atggaggccc tgccgcccct cttcccgacg aagatcctcc gtgcgccttg gggagccagc
     5941 gcaaggatct cgcgggcgat gaggtcgtcg tagaccacgg catcgcaccc ggagatgatg
     6001 cgcgtcgcct tgagcgtcac cagctcgggg tctcccgggc cggcgccgat caggtaaacc
     6061 tttgcgggtg agaccttgtc aggcatccat cgaacctctt ctcttgacat ccaccaggag
     6121 cgcgcgggcg agctcggcac ccacccgggg cccgtcgccc tccatctcgc gccgggtgca
     6181 gaagcgacag tccgcggagc cgagcatgcc gaccatggct atcgagccgc cctcgagccg
     6241 cgccagggcg cctgcgggca gcctgcagtc ctggccgagt tcggcgagga aggcgcgctc
     6301 cgcgagggcg cacgtcctcg tctgggcgtg gtctatggac gacaggagac ccatcgtctc
     6361 gtcgtcgccc accctgcact cgaccgcgat gacgccctga ccgggtgacg gtaccatgac
     6421 ccccgggtcg agggcaacac caggcctcaa ccccagcctc gccacgcccg cggccgcgag
     6481 gaccacggcg tcgaccgtcc tgcccagcct gttcaggcgc gtctggacgt tccccctgat
     6541 gtccactacc tggagatcgg gccttgcgtt cctgatctgg cacttcctcc tgaggctcga
     6601 ggtccccacc ttggagcctt cggggagctc ctcgagcccc agtcccgagg aggagaagac
     6661 catgtccctg ggatcctccc gctcgatgta tgcgccgata acgaggccgt ccgggaggtg
     6721 tgcctgcatg tccttgaggc tgtgcaccgc gatgtcgatg gacccatcca gcagtgcgcg
     6781 ctcgatgtcc ttcacgaaca cccccttccc gccgatgacg tcgagcggcc tgtccgagag
     6841 gatgtccccg gtcgtcttga tgcggaccac ctcgaaggcg accccgggcg ccttcttccc
     6901 gagcgcgtcc atgacgagcg ccgtctgcgc cagggcgagc ctgctacccc ttgtgccgat
     6961 cctcatgatc ctcatccagc ctgaatatcc tccgtacgta ctctatgtgc ctggcgtcgg
     7021 ggtgctcctt caggaagctc acgggcctgt ggagcatgcg cctcaaggag gtccgcagcg
     7081 cctgttccac gaccgtcgcg tccaccttgc tcgagacctc tctcgcctgc tccacggcga
     7141 tcgcgtcggc cagcgcatag agatccctga tggtcgcctg cgcggacagg gaacgcaacc
     7201 accgctcgaa cgtcgcgacc tcttcctcga tgatggccat ggccctcgcc gcctccgcct
     7261 cgcgctcggc gaagtgcctg tccacgatgg cctggagcgc gtcgatgtcg tacaggtagc
     7321 agttgtaaca ctctttcgcg ccctcgtcga cgtccctcgg gagggcgatg tcgacgatga
     7381 tcaggggcct gttcctcctg atgcccatgg cccgctgcac catctccctc gttatgatgg
     7441 gcctggccgc cccggtcgag gtgatgacga cgtcgctcga tgcgagctct
//
LOCUS       DAOSCR010000040         7455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6399_length_7455_cov_3.458649, whole genome shotgun sequence.
ACCESSION   DAOSCR010000040 DAOSCR010000000
VERSION     DAOSCR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6399_length_7455_cov_3.458649"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..756)
                     /locus_tag="PLT69_01945"
     CDS             complement(31..756)
                     /locus_tag="PLT69_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOJ13243.1"
                     /translation="MKNLLEDLIALQQVELEIFKAEEGLRELPKNIEEIESIIQARKR
                     SLDAIDEEIASYEQRKEPLEAELKENQAILDAADARIKRIKTNKEFLALQREIDLAKK
                     RKADLEEQILSIMERIEKKSMERERIRESFNADRAVLEERKAKVEQQIRELEAVLADY
                     RGKDKALREGVDPSLLSRYDRIKNSKKGLAVVACVDGVCRGCHMHIQPQLFNELVRGD
                     KLITCPSCQRILYAETLDDHEDH"
     gene            complement(753..1520)
                     /locus_tag="PLT69_01950"
     CDS             complement(753..1520)
                     /locus_tag="PLT69_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ13244.1"
                     /translation="MRLSDVLAVLNSIAPFDAAEAWDNIGLQVGDPTMEVRSILVALD
                     PTLEALGMASTNGCELVVTHHPLIMTPLGRIDLAETVSRKIELLLRQKLGLVCMHTNL
                     DKAPGGTADMLAEALGLKDASGQGFLRVGKAGTKTPLSLWVEGLPFRAARLCDAGRLV
                     ETVGACPGSGMELWRQARSLGCDTYVTGDVRYHAALEAVEAGMNVVDLGHFATEEIVL
                     DSLAGRLGAELCGIRVIVHKGRDVFTAYEKTNRGEAH"
     gene            complement(1641..1713)
                     /locus_tag="PLT69_01955"
     tRNA            complement(1641..1713)
                     /locus_tag="PLT69_01955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1678..1680),aa:Ile,seq:cat)
     gene            complement(1759..3525)
                     /locus_tag="PLT69_01960"
     CDS             complement(1759..3525)
                     /locus_tag="PLT69_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13245.1"
                     /translation="MDIRDRVPVIRVAIRGANGRMGLQAIYASMENNEDILARAKTKE
                     LTDKDVWIDVVFGAFTADIPTVQQLMAGVMRSEKAKILEGYDKKGSPIYRVKSDGKFE
                     LRTRYVEQTLDEEPQRRHVIPFDRQELVDSPEGLPAIKILSRDKQEVLSVIRHANVRL
                     GELSAEAIMDRVLDTANDLGAHVLLSCVGDTAKKADRMQMWLDKIHEKNYAIGFLESA
                     PAKGWDKRILSRQDQISVLCSRNFSPTGTLFRTGIALKQPLFDYNPYEFGKAAERFKK
                     IFTRIVENVIATDMFKDFAQILKKTPTTIREIDGEEILVNFPPDFLTITSPSYLVFSF
                     YEDNEYGFICAALIPNLKALGLIYRDAALVEKIRKNGHRYEAVTNIPGLFGTDWMVKA
                     PAEIIARGGAMSTSSCTTNGNIVGDTIVMLALHCYYDFFAEAAKDYFTASDSTARSKA
                     EEKIEKRFSDFKGYLSKLLAKRSTMIKTVFTFYYDQVAFMNTDMQHGLTRSESPEVLA
                     FLRETSSGSQTEVPRLLSLPAREKEYLTAQKAVMEKQASREKDEQEKARLLDEMKRID
                     ASLKSLDTSIDIVDIIEKTEGK"
     gene            3673..4806
                     /locus_tag="PLT69_01965"
     CDS             3673..4806
                     /locus_tag="PLT69_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ13246.1"
                     /translation="MLSVDIAIVGAGVVGLAVAGAVAGSSRAVVVLEQEAGPGRHTSS
                     RNSEVIHAGIYYPTGSLKASLCVRGARMLYDFCVRHGIAHKKTGKVIVAASHAEEPGL
                     EALLQRGMENGVEGLALITQAELGRIEPNVRGTCALSSPHTGILDTHGLLRRLEAMAA
                     DSGCTILYRTRLCAVTPVKDGFVCRVEGPDGQEYSFASRVVVNAAGLWADHVALLAGI
                     DCDSAGYRIHKVKGQYFRVRRSKQALVHGLVYPSPETDLVGLGIHATKDLSGALRLGP
                     DASYVDTIDYDVDSGAAGVFLDRARTILPFLEEGDLHPDTAGIRPKLQAEGGPFRDFE
                     IRHEADRGLPGMITLAGIESPGLTSCLSIAELVKGMVEDSGLL"
     gene            complement(4803..6077)
                     /locus_tag="PLT69_01970"
     CDS             complement(4803..6077)
                     /locus_tag="PLT69_01970"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOJ13247.1"
                     /translation="MIVKGGRLIDPASGRDALADIVIAGGRIEGIFPPGKAKGGGGET
                     IDATGLWVLPGLIDMHAHLREPGQEYKEDIASGSAAAAAGGITTVVCMANTDPVNDNP
                     TVTRSIIDRARAVGLARVLPAGAITMGLEGRRLAEMGLMKRSGIVAVSDDGRPVEDAS
                     VLRRALEYASTFGLPVILHSEDKSLAGKGVMNEGPLSARLGLPGIPAAAEEAMVCRDL
                     AVARYAKPPVHITHVSTGGALAAIGACKETSLGMTCDVTPHHLLLTEEALSAYDTVFK
                     VNPPLRTEKDRLALIEGIRNGTVDCIATDHAPHAADEKALDFDLAPFGVIGFQTLLPA
                     IMKIHLEHDIPFMRLLACVTTNPARILGIEGGVLFEGARADLVLFDPKAVWTLTEDRV
                     LSKSKNSPFLGWQFTGKVVRTLLEGRTVHVAD"
     gene            complement(6067..6999)
                     /locus_tag="PLT69_01975"
     CDS             complement(6067..6999)
                     /locus_tag="PLT69_01975"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOJ13248.1"
                     /translation="MEWSRKDLVSIAELSAEEILTVLDVSATFKEINRREIKKVPTLR
                     GKTVVLLFYEPSTRTRLSFELAAKRLSADTVALSVSGSSFLKGETIMDAAMNVMAMIP
                     DCIVIRYPYAGLPARLARELSCSVINAGDGAHEHPTQALLDLCSMRERFGRLAGLHVA
                     MVGDIAHSRVARSNVYALKKVGARVSVVAPKTLLPQDISTWGVEVWHEFDPVVKDADV
                     LMMLRVQKERMDEQCFPDEREYAHFYGLDRRRVDMMKPGAVIMHPGPVNRGVEITHEA
                     ADGSRSIILNQVENGVAVRMALLYLCLGGSADDR"
     gene            complement(6999..>7455)
                     /gene="pyrR"
                     /locus_tag="PLT69_01980"
     CDS             complement(6999..>7455)
                     /gene="pyrR"
                     /locus_tag="PLT69_01980"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOJ13249.1"
                     /translation="VSSGAPIAFVAIRKGGEILAGRLVELLEERTNIRVPLGVLDITL
                     YRDDITRRRIYEVKATDIPFDVSSQDIVLVDDVIQTGRSARAAIDHIMDLGRPRRIYL
                     VVLFDRGGRELPIQPDITGMKVDLPEDRTIKIEVDDRGAISGLIMGGVK"
BASE COUNT         1264 a         2424 c         2215 g         1552 t
ORIGIN      
        1 cgtgaagcgg gcatgctcgt ggagacgagg tcagtggtcc tcgtgatcgt ccagggtctc
       61 ggcgtagagg atcctctggc aggacgggca cgtgatgagc ttgtctccgc gcacgagctc
      121 gttgaagagc tgcggctgga tgtgcatatg gcacccgcgg cacacgccgt ccacacacgc
      181 caccacggcc aggcccttct tgctgttctt gatccggtcg tatctcgaca ggagcgaggg
      241 atccacccct tcgcgaagag ccttgtcttt cccccggtag tccgcgagca cggcctcgag
      301 ctccctgatc tgttgctcaa ccttggcctt tcgctcctca aggacggcgc ggtccgcgtt
      361 gaaggactcc ctgatgcgct cccgttccat gcttttcttc tcgatgcgct ccatgatgga
      421 caggatctgt tcctcgagat ccgccttgcg ctttttcgcg agatcgattt cccgctggag
      481 ggcgagaaac tccttgttgg tctttatcct ctttattctc gcatcggcgg cgtccaggat
      541 cgcctggttt tccttgagct ccgcctccag cggctctttg cgctgctcgt aggatgcgat
      601 ctcctcgtcg atggcatcaa gggatcgctt gcgggcctgt atgatgctct cgatctcttc
      661 gatgtttttg ggaagctctc tcaacccctc ttcagccttg aagatttcca gctccacctg
      721 ctgaagggct atcagatctt cgagtaagtt tttcaatgtg cctctcctcg attcgttttc
      781 tcgtaggcgg tgaagacgtc cctgcccttg tggacgatca cccgtatgcc gcacagttct
      841 gcgcccagcc tccctgcgag gctgtcgagg acgatctcct ctgtcgcgaa atggccgagg
      901 tcgacgacgt tcatccctgc ctctacggcc tcgagggctg cgtggtagcg tacatccccc
      961 gtgacatagg tgtcgcatcc gagcgagcgg gcctggcgcc acagctccat cccgctcccg
     1021 gggcatgcac ccacggtctc aacgagcctt cccgcgtcgc acaagcgcgc agcccggaag
     1081 gggaggccct ccacccataa agacagcggc gtcttcgttc ccgccttccc aaccctcagg
     1141 aacccttgac ctgaggcatc cttcaagccg agcgcctccg cgagcatatc ggccgtgcct
     1201 cccggggcct tgtcgaggtt cgtgtgcatg cacacgagcc cgagcttttg cctcaagagg
     1261 agctctatct tcctggacac ggtctcggcc aggtcgatcc tccccagggg ggtcatgatc
     1321 aggggatggt gggttaccac cagctcacac ccgttggtcg acgccatgcc gagggcttcg
     1381 agggttggat caagcgccac gagtatcgag cgcacctcca tggtggggtc gcccacctgc
     1441 agcccgatgt tgtcccacgc ttctgcagcg tcgaacggcg ctatcgagtt caacaccgcc
     1501 aggacgtccg agagtctcat gggcgatgta aaaaggggtg ctctcgaaga gcacccctca
     1561 tggaccttcc ggtcagtctg ctcgtagcaa cacagtcttt gatcgccgcc ttgctcaccc
     1621 ttgttccttt ggcccattga tgggcccacc tgggatcgaa ccagggacct accggttatg
     1681 agccggtggc tctaccaatt gagctatggg ccctctgtca agaggttctt agtctaaacg
     1741 aaaaaaaggt gtcaaggctt atttgccctc ggttttctcg atgatatcca cgatatcgat
     1801 gctcgtatcg agggatttga ggctcgcatc tatcctcttc atctcgtcga ggagccttgc
     1861 cttttcctgt tcgtcctttt cgcgcgaggc ctgcttctcc atcacggcct tctgcgcagt
     1921 gaggtattcc ttctccctcg cgggcagcga cagcagtctc gggacctctg tctgggagcc
     1981 ggaagaggtc tccctgagga acgccaacac ctctggagac tcggagcggg tgaggccgtg
     2041 ctgcatgtcg gtgttcatga atgcgacctg gtcgtagtag aaggtgaaca cggtcttgat
     2101 catggtggaa cgtttcgcaa ggagtttcga caggtacccc ttgaaatccg agaacctctt
     2161 ctcgatcttc tcctccgcct ttgacctggc ggtggaatcc gacgccgtga agtagtcctt
     2221 cgccgcctcg gcaaagaagt cgtagtagca gtggagggcc agcatcacga tcgtgtcccc
     2281 gacgatgttg ccgttggtcg tgcaggaact cgtggacatc gccccgcccc gcgctatgat
     2341 ctcggcgggc gccttcacca tccagtccgt tccgaagagc ccggggatgt tggtgaccgc
     2401 ctcgtaccga tggccgttct tcctgatttt ctccacgagg gccgcgtcac ggtagatgag
     2461 ccccagggcc ttgaggttcg ggatgagcgc cgcgcagatg aacccgtatt cgttgtcctc
     2521 atagaaactg aacaccaggt aggaaggcga ggtgatcgtg aggaagtccg gcgggaagtt
     2581 caccaggatc tcctcgccgt ctatctccct tatggtggtg ggggtcttct tgaggatctg
     2641 tgcgaaatcc ttgaacatgt ccgtggctat cacgttctcg acgatcctgg tgaagatctt
     2701 cttgaaccgc tccgccgcct tgccgaactc gtagggattg tagtcgaaca gcggctgctt
     2761 cagggcgata ccggtcctga agagggtccc tgtggggctg aagttcctcg agcacaagac
     2821 gctgatctgg tcctgcctcg aaaggatcct cttgtcccat ccctttgccg gggcggattc
     2881 gagaaagccg atcgcgtagt tcttctcgtg tatcttgtcg agccacatct gcatgcggtc
     2941 ggccttcttg gccgtgtcgc ccacgcagga aaggagcacg tgggcgccga gatcgtttgc
     3001 ggtatccagc acccggtcca tgatggcctc ggccgagagc tcgcccaggc gcacgttggc
     3061 gtgcctgatg accgagagca cctcctgctt gtcccgagag aggatcttga tcgcaggaag
     3121 cccctcgggc gagtcgacca gttcttgcct atcaaaaggg atgacgtgcc ggcgttgcgg
     3181 ctcctcgtcg agcgtttgct ccacgtacct ggttctaagc tcgaacttcc cgtcagactt
     3241 gacccggtaa atgggactcc ccttcttgtc atacccctcg agtatctttg ccttctcaga
     3301 cctcatgact cccgccatga gctgctgcac cgtcggtatg tcagcggtga acgccccgaa
     3361 aacgacatcg atccagacgt ccttgtcagt gagttccttg gtcttggccc tcgccaggat
     3421 atcctcgttg ttttccatgg acgcgtagat agcctgaagg cccatcctcc cgttggcacc
     3481 cctgatggcg accctgatca ccggaaccct gtccctgata tccatatctt cctccgaaga
     3541 atgtgtggtg tggtgtgccc gacgagtgcg aacacaacta gatataccaa aacgcaggcc
     3601 gcgtggcaag gaggcctccc tgcctctcca ggggcctttc ccctttcgtg tctttcctgc
     3661 tatacgaact tcatgctctc cgtggacatc gcgatcgtcg gtgccggtgt ggtcggtctc
     3721 gctgtggcgg gcgccgttgc cgggtcatcg agggccgtgg tggtcttgga gcaggaggcg
     3781 ggcccgggac gtcacacgag ctcgcgcaac tccgaggtca tccatgccgg gatctactac
     3841 cccacgggca gtctcaaggc cagcctctgc gtgcgggggg cccgcatgct ctatgacttc
     3901 tgcgtacgcc acgggatcgc ccacaagaaa acaggcaagg tcatcgtggc cgcgagccat
     3961 gccgaggaac ccggcctcga ggccctcctg cagagaggca tggaaaacgg cgtggagggt
     4021 ctcgccctga tcacccaggc cgagctcggc cggatcgagc cgaacgtgag ggggacatgc
     4081 gccctctcat cgccccacac cggcatactc gacacccatg gcctcctgag gcgcctggag
     4141 gccatggccg ccgacagcgg gtgcaccatc ctgtacagga cccggctgtg cgccgtcacc
     4201 cctgtcaaag acggttttgt gtgcagggtc gaggggccgg acggccagga atactctttc
     4261 gcctcccgtg tggtcgtgaa cgcggccggc ctgtgggcgg accacgtggc ccttcttgcg
     4321 ggcatcgact gcgacagcgc aggataccgg atacacaagg tcaaggggca gtatttccgc
     4381 gtccgcaggt ccaagcaggc gctcgtccac ggcctcgtct acccgagccc tgagacggat
     4441 ctcgtggggc tcggcatcca cgccaccaag gacctctcag gggccctcag gctcggtcct
     4501 gatgcgagct acgtcgatac catcgactac gatgtggaca gcggcgccgc cggggtattc
     4561 ctcgatcggg cgcgaacgat cctgccgttc ctcgaagaag gggacctcca cccggacacg
     4621 gcgggtatac ggccgaaact ccaggccgaa ggcgggccgt ttcgcgactt cgaaatccgg
     4681 cacgaggccg accgcggact gcccggcatg atcacgctcg cgggaataga gtccccgggg
     4741 ctcacgagct gtctctccat cgccgaactc gtgaagggga tggtcgagga ttcagggctc
     4801 ctctagtcgg cgacgtggac tgtgcggccc tcgagcaggg tccggaccac cttgccggtg
     4861 aattgccagc cgagaaaggg tgaattcttc gacttggaga ggaccctgtc ctcggtgagg
     4921 gtccagactg ccttagggtc gaacaggacg aggtcggccc tcgccccctc gaagagcacc
     4981 ccgccctcga tacccaggat tctcgcgggg ttcgtcgtga cgcacgcaag cagcctcatg
     5041 aagggaatgt cgtgttcgag gtgtatcttc atgatcgccg gcagcagggt ctggaaccct
     5101 atcaccccga acggcgcaag gtcgaaatca agggccttct cgtccgcggc atgcggtgca
     5161 tggtccgttg cgatgcagtc cacggtcccg ttcctgatcc cctctatgag ggcgagcctg
     5221 tctttctccg tcctcagcgg cgggttcacc ttgaagaccg tgtcataggc ggaaagcgcc
     5281 tcctcggtga gcagcagatg gtgaggcgtc acgtcgcagg tcattcccaa ggaagtctcc
     5341 ttgcacgccc cgatcgcggc cagggccccg ccggtcgaca catgggtgat gtggaccggg
     5401 ggcttcgcgt acctggctac cgcgagatca cggcacacca tcgcctcctc cgcggcggca
     5461 gggatccctg gaaggccgag gcgggccgaa aggggcccct cgttcatcac ccccttgcct
     5521 gccagggact tgtcctcgga gtggagtatg accggcaggc cgaaggtgga ggcgtactcc
     5581 agggcacgtc gcaagaccga cgcgtcttcg accggcctcc cgtcgtccga gacggcgacg
     5641 atacccgagc gcttcatgag ccccatttcc gccaggcgcc ttccctcgag gcccatcgtg
     5701 atggcacccg ctgggagcac cctcgcgagc ccgaccgcac gcgccctgtc tatgatggag
     5761 cgggtcaccg tggggttgtc gttgaccggg tcggtgttcg ccatgcacac gaccgtggtt
     5821 atcccacctg cagccgcggc cgcgctccct gatgcgatgt cctccttgta ctcctgcccg
     5881 ggttccctga ggtgggcatg catgtctatg agcccgggca ggacccacag gccggtcgca
     5941 tctatcgttt cgcccccgcc ccccttcgcc ttgccaggcg ggaagatccc ctctatcctg
     6001 ccgccggcga tcacgatatc cgccagggcg tcccgcccgc tcgccgggtc tatcaacctc
     6061 ccacccttaa cgatcatctg ccgatcctcc gaggcacagg tacaagaggg ccatccgaac
     6121 cgccaccccg ttctccacct ggttgaggat gatcgagcgg ctcccgtccg ccgcttcgtg
     6181 cgtgatctcc acgccgcggt tgacgggccc cgggtgcatg atcacggccc cgggcttcat
     6241 catgtcgacg cggcgcctgt caagcccgta aaagtgggca tattcgcgct cgtcggggaa
     6301 gcactgttcg tccatgcgct ccttttgcac cctgagcatc atgagaacgt ccgcgtcctt
     6361 caccacgggg tcgaactcgt gccacacctc caccccccac gtggatatat cctgggggag
     6421 cagggtcttc ggggcgacga cgctcaccct cgcgcccacc ttcttcagcg cgtacacgtt
     6481 ggagcgggcc acacgcgagt gggctatgtc cccgaccatg gccacgtgaa gccctgcgag
     6541 cctcccgaag cgttcccgca tggagcagag atcgaggagc gcctgtgtcg ggtgctcgtg
     6601 cgcaccgtcg cctgcgttga tgaccgagca ggaaagctct ctcgcgagcc tggccggcag
     6661 ccccgcatag ggatacctga tgactatgca atcagggatc atggccatca cgttcatggc
     6721 cgcatccatg atggtctccc ccttgagaaa ggaagaaccc gatacggaca gggcgaccgt
     6781 gtccgcgctg agccttttgg ccgccagttc gaaggaaagc cgcgtgcggg tggacggctc
     6841 gtagaacagc aagaccacgg tcttccccct gagcgtgggg accttcttga tctccctccg
     6901 gttgatctcc ttgaaggtgg cgctcacgtc caagacggtg aggatttctt ccgcgctcag
     6961 ctccgctatg gacaccagat ccttgcggga ccattccatc acttcacccc gcccatgatc
     7021 aagccggata tcgccccccg gtcgtccacc tcgatcttga tggtacggtc ttccggcagg
     7081 tccaccttca tgcccgtgat gtcgggctgg atgggcagtt ccctcccgcc cctgtcgaac
     7141 agcacgacca ggtagatccg ccttggcctt ccgagatcca tgatgtggtc tatggcggcc
     7201 cgcgccgatc gcccggtctg gatcacgtca tccacgagga cgatgtcctg ggatgagacg
     7261 tcgaagggga tgtcggtggc cttcacctcg tagatcctgc gccgcgtgat atcgtcgcgg
     7321 tacagcgtga tgtcgagcac gccgagggga accctgatgt ttgtgcgttc ctcgagcagt
     7381 tccacgagcc tgccggccag gatctccccg cccttcctga tcgccacgaa ggcgataggc
     7441 gcgccgctgc tcacc
//
LOCUS       DAOSCR010000041         7411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6439_length_7411_cov_3.721316, whole genome shotgun sequence.
ACCESSION   DAOSCR010000041 DAOSCR010000000
VERSION     DAOSCR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6439_length_7411_cov_3.721316"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /gene="guaB"
                     /locus_tag="PLT69_01985"
     CDS             complement(<1..1325)
                     /gene="guaB"
                     /locus_tag="PLT69_01985"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOJ13250.1"
                     /translation="MEKILAEALTFDDVLLLPGKSDVLPSMVDITTWFTKGIKLNIPL
                     VSAAMDTVTEAATAIAMAREGGIGIIHKNMTPEQQAVEVDKVKKSESGMIVDPITMRP
                     DQTIGEALKLMMHYRISGVPITEEGRLIGILTNRDLRFETDMSKPIEQAMTRENLITV
                     EGGISLEEAKKLLHRYKIEKLPVVDKDYRLKGLITIKDIEKAQKYPNSAKDSKGRLLV
                     GAAVGVMPADEQRLHVLMKAGVDVIVIDTAHGHSRGVIDSVKFIKSLYPQAQVVAGNV
                     ATAEATADLIAAGADGVKVGIGPGSICTTRVVAGVGVPQITAIIECAKVARKHGVPLI
                     ADGGVKFSGDLTKGLAAGASTIMVGSLFAGTDEAPGERIIYQGRTYKAYRGMGSVEAM
                     RAGSKDRYFQEDVSDTEKLVPEGIVGRVPYRGPISENVYQLLGGLRAGMG"
     gene            complement(1340..2215)
                     /gene="sppA"
                     /locus_tag="PLT69_01990"
     CDS             complement(1340..2215)
                     /gene="sppA"
                     /locus_tag="PLT69_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOJ13251.1"
                     /translation="MKRRTTWILIILVILILAGWGAGRAFKALKPAVGILEIDGVITE
                     SVVYLDAIRDFERDDTVKAVVVRLDSPGGKVGPSQEIHAALASLRRKKPVVASLGALG
                     ASGAYYIACAADTVYALPGTMTGSIGVIMEFFDASEGLRRVGLQAKSVTSGALKDAGS
                     PFRPMTDAERAYFKDLVDDVHAQFVDAVSKGRRLKPERVKALADGRIFTGRQAMGLGL
                     VDRLGGLDDAVREAGSRASIEGEPRIVKAGDQKGAWDMVKRFLKTTGPALPGAVRLFE
                     EGRTRGSVSLEYSIP"
     gene            complement(2484..4259)
                     /locus_tag="PLT69_01995"
     CDS             complement(2484..4259)
                     /locus_tag="PLT69_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOJ13252.1"
                     /translation="MVSVDETKNGPKDEGHATQDVSAGDSEVNETGGADFEALYEETF
                     DKRITPGSLVTGTVVQIGTDFIMIDIGRKIDGQAPISEFLGEDGTLNCSVGQEVEVLV
                     ESINTTKGIIRLSKQKAERLKVWDDVVKASQENTYLRGRVKERIKGGLIVDIGLNAFL
                     PSSQASLGPVSDAELESMIGRVIDVAVIKFNRKKNNVVVSHREVLEKQREENKKRLLA
                     TLAKGDVVTGKVKNIMSYGAFVDIGGIDGLLHITDMSWGKLRDPKEKVSTGQEIRVKV
                     LEFDPETEKISLGMKQLQPDPWEGLEYRYPIGKKVPGKVTSVTNYGAFVELEDGVEGL
                     VHISDMFWTKRMRHPSTILSEGQEVEVVVLGVDLANRRISLGLKQTMPNPWSQIRENY
                     PEGAVVNATVKNVTDFGLFVSVDEDIDVDGLIHVSDISWDPKVKNPKELFKKGDVVKA
                     KVLAVDPENEKFSLGIKQLQPDPWHELAMEHPVGSVVKGKITSLTDFGAFVEIRPGIE
                     GMVHISEVSSDKISSLADVLSVGQEVEAVVLRINPDNKKIGLSIKALEEGYHRRSAPQ
                     QEKGPEKVGTNLGDLLKGFKEGKKS"
     gene            complement(4510..4932)
                     /locus_tag="PLT69_02000"
     CDS             complement(4510..4932)
                     /locus_tag="PLT69_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13253.1"
                     /translation="MLPRDLYSQAHLFVAAVRVHEHVHGRPPSLGDLSGILGVSEEEL
                     SLLSRRLEERGIVRVIASGGQERFTTGDHTRIENLPRSAEAPALEDEISRFKARQETR
                     MRDLEAALKGSPDKRSVFSEIEKALKDPTLAKKKNPLD"
     gene            5058..6074
                     /locus_tag="PLT69_02005"
     CDS             5058..6074
                     /locus_tag="PLT69_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOJ13254.1"
                     /translation="MRKPSLPIVFLAVVVFLAFRGTSDLRADETTHTVVKGDTLWDIS
                     MKYLKTPWKWPLVWANNQDITNPHLIYPGDRVIITTEGGRTILKIIPSPERGGEEREA
                     VFYTPREIASVKDKSVVLAPQFALYMFSPNLLKGSGEIVRKVENGELISENDHIVISS
                     GSAFKKDQVVTIVTKIQDIVDEEGDSAGYLYRITGMARVDEVVGGLVKATVTYSLQES
                     RIGSIIFDDIPPIKPVALDISEPSGISGVVMDHHGGVVGGATFDLVFVNAGTRQGVEK
                     GALLSVYRKTDALGGDANRQAASFHDPIGVIVVLQSLDSSSMGLVVQSKELIEKGALV
                     LGRK"
     gene            complement(6332..6640)
                     /locus_tag="PLT69_02010"
     CDS             complement(6332..6640)
                     /locus_tag="PLT69_02010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ13255.1"
                     /translation="MKDRRAHKRVKVDLRAAYKDDGFVLRTGRVLNLGKGGMYLETGR
                     GVALGGFVDASIDDREFGKVISVRGRVAHKTPDGVGVTFVRVDRLGLMNILTYHGAPF
                     "
     gene            6994..>7411
                     /locus_tag="PLT69_02015"
     CDS             6994..>7411
                     /locus_tag="PLT69_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ13256.1"
                     /translation="MRKLYDALAISRKVSQLGEEISRSYMGKELLVVGILKGGFMFMS
                     DLVRAVSLPTRIDFARLSSYGKSDSPEGDVKIVDDVHEDIQGVHVLVVDDIMDTGQSI
                     TAFKEHLESKGPASVKICTMINKKFRRSKDIDPDYSG"
BASE COUNT         1274 a         2516 c         2147 g         1474 t
ORIGIN      
        1 cccatgccgg cacggagccc gcccaggagc tggtagacat tctccgagat ggggccccgg
       61 tacggcacgc ggcccacgat cccctcgggc acgagcttct cggtatccga gacgtcctcc
      121 tggaagtagc ggtccttcga ccccgctcgc atcgcctcca ccgaacccat gccgcggtag
      181 gccttgtacg tccttccctg gtagatgatc ctctcgcccg gcgcctcgtc cgtcccggcg
      241 aagagtgaac ccaccatgat cgtcgaggcc ccggcagcca gccccttggt caggtcgccc
      301 gagaacttca cgccgccgtc ggctatgagg gggaccccgt gtttcctcgc caccttggcg
      361 cactcgatga tcgccgtgat ctgggggacg cccaccccgg ccacgacgcg ggtcgtgcag
      421 atggaaccgg ggccgatgcc caccttgacg ccgtccgccc cggcggcgat gaggtcggcg
      481 gtcgcctcgg ccgtggcaac gttcccggcg accacttggg cctgagggta gagcgacttg
      541 atgaacttca cggaatcgat cacgcccctg ctgtgcccgt gggccgtgtc tatcacgatc
      601 acgtccacgc cggccttcat caagacgtgg agccgctgct cgtccgccgg catcaccccc
      661 acggccgcac ccaccaggag cctgcccttt gagtccttcg ccgagttggg gtacttctgc
      721 gccttctcga tatccttgat cgtgatgagc cccttgaggc ggtagtcctt gtccaccacc
      781 ggcaacttct cgatcttgta acggtggagg agcttcttgg cctcctccag gcttatgccc
      841 ccctcgacgg tgatcaggtt ctccctggtc atcgcctgct ctatgggctt gctcatgtcg
      901 gtctcgaacc tgaggtccct gttcgtgagg atgccgatga gcctgccctc ctccgtgatg
      961 ggcacgccgg atatcctgta gtgcatcatg agcttgaggg cctcgccgat ggtctggtcg
     1021 ggcctcatgg tgatggggtc aactatcatc ccgctctcgg acttcttgac cttgtccacc
     1081 tccacggcct gctgttccgg cgtcatgttc ttgtggatga tcccgatgcc gccctcgcgc
     1141 gccatcgcga tcgcggttgc ggcctcggtc accgtgtcca tggcggcgct caccagcggt
     1201 atgttcagct tgatcccctt cgtgaaccag gtggtgatgt cgaccatgga ggggagcacg
     1261 tctgacttgc cgggcagcaa caggacatcg tcgaacgtca acgcctcagc gaggatcttc
     1321 tccatgagtt cacgtctcct tacggtatgc tgtactcgag gctcacgctc cccctcgtcc
     1381 gcccttcctc gaagaggcgg accgcaccgg gcagggccgg ccccgtcgtc ttgaggaacc
     1441 tcttgaccat gtcccaggcc cctttctggt ccccggcctt cacgatcctg ggctcgccct
     1501 ctatggacgc cctgctcccg gcctccctca cggcgtcgtc gaggccgccg agcctgtcaa
     1561 cgagcccgag tcccatggcc tgccgccccg tgaagatcct cccgtcggca agcgccttga
     1621 ccctctccgg cttcagcctg cgccccttcg agacggcatc cacgaactgt gcgtgcacgt
     1681 cgtccaccag gtccttgaag taggcgcgct cggcgtcggt catgggcctg aagggcgagc
     1741 ccgcgtcctt gagggccccg gacgtgacgc tcttcgcctg gagacctacc cgcctgaggc
     1801 cttcgctcgc gtcgaagaat tccatgatga ccccgatgct gccggtcatg gtgccgggga
     1861 gcgcatagac cgtgtccgcg gcgcaggcta tgtaataggc ccccgatgcg ccgagggccc
     1921 ccaaagacgc gacgaccggc ttcttgcgcc tgagcgacgc taaggcggcg tgtatctcct
     1981 gcgacggtcc caccttgcct cccgggctgt cgagcctcac caccaccgcc ttgacggtgt
     2041 cgtcacgctc gaaatcccgt atggcatcca ggtacacgac ggactcggtg atcacgccgt
     2101 ctatctcgag gatgcccacg gcgggtttca gcgccttgaa cgcgcggccg gcaccccacc
     2161 ccgcgaggat gaggatgacc aggatgatga ggatccatgt agtcctgcgt ttcatgcctc
     2221 gctcgatcga atgggcgaat gcctttttca ggaccccgta gacgcagggc gtcttcgccg
     2281 agccccgcaa cggggatgca aagactatat aggagattct ccgggttgtc caccggcctc
     2341 gaagacctgc acggggtcac gtgcggcaac cgtgaccgcc gcagggtgcc tggtggcccc
     2401 gcacggtctc cccgctgtcc gggcgaggcg cgcgccccgt ccttcgggcc cgcgcagagc
     2461 cgcagccctc tcccccggag accctaactc ttcttgcctt ccttgaaccc cttgagaagg
     2521 tctcccaggt tcgtcccgac cttttccggg cctttttcct gctgcggcgc agatctcctg
     2581 tggtacccct cttccagggc cttgatcgaa aggcctatct tcttgttgtc ggggttgatc
     2641 ctgaggacga cggcctccac ctcctggccc acggacaaca cgtccgccag cgagcttatc
     2701 ttgtcgctgc tcacctcgga tatgtggacc atgccctcga tcccgggcct gatctccacg
     2761 aacgcgccga aatctgtcag cgaggtgatc ttccccttga ccacgctccc caccgggtgc
     2821 tccatggcga gttcgtgcca cgggtccggc tggagctgct tgataccgag ggagaacttc
     2881 tcgttctcgg ggtccaccgc caggaccttc gccttgacca cgtcaccctt cttgaagagc
     2941 tccttggggt tcttgacctt cgggtcccag ctgatgtccg acacgtggat gaggccgtcc
     3001 acgtcgatgt cctcgtccac gctcacgaac aggccgaagt ccgtgacgtt cttgaccgtg
     3061 gcgttcacga cggcgccctc ggggtagttt tccctgatct gggaccatgg gttgggcatg
     3121 gtctgcttga ggcccaggga gatacggcgg ttcgcgaggt ccacaccgag gacgaccact
     3181 tccacctcct gtccctcgct caggatggtc gacgggtgcc tcatgcgctt ggtccagaac
     3241 atatcggaga tgtggacgag tccctccacc ccgtcctcga gctcgacgaa ggcgccgtag
     3301 ttggtcaccg aggtgacctt gccggggacc ttcttgccga tggggtacct gtactcgagc
     3361 ccctcccatg gatccggctg cagctgcttc atgcccaggg agatcttctc ggtctcgggg
     3421 tcgaactcca gcaccttcac cctgatctcc tgtcctgtgg agaccttttc cttgggatcc
     3481 ctgagcttgc cccaggacat gtccgtgatg tggaggagcc cgtcgatgcc gcctatgtcc
     3541 acgaaggcgc cgtagctcat gatgttcttg accttgcccg tcaccacgtc gcccttggcc
     3601 aaggtcgcca gcaggcgctt cttgttctcc tcgcgctgct tttccagcac ctcgcggtgg
     3661 ctcaccacca cgttgttctt cttgcggttg aacttgatga ccgcgacatc gatgacccgg
     3721 ccgatcatgc tctcgagctc ggcatcgctc accgggccga gggacgcctg cgacgagggg
     3781 agaaaggcgt tcaggcctat gtcaacgatg agcccgccct tgatcctctc cttcaccctg
     3841 cccctaaggt aggtgttctc ctgggaagcc ttgacgacat cgtcccagac cttgagcctc
     3901 tccgccttct gcttgctgag ccggatgatg cccttggtgg tgttgatgct ctcgaccagg
     3961 acctcgacct cctgccccac cgagcagttg agcgtcccgt cctcgccgag aaactcgctg
     4021 atgggtgcct gaccgtcgat cttcctgccg atgtctatca tgataaagtc ggttccgatc
     4081 tggaccaccg ttccggtcac cagggaaccg ggcgttatgc gcttgtcgaa ggtttcttcg
     4141 taaagggcct cgaagtccgc acctccggtt tcattcacct cggaatcacc agccgaaaca
     4201 tcctgcgtcg catggccctc gtctttgggc ccgttcttgg tctcgtccac gctcaccata
     4261 gtttccttcc cccgttccgt cgttccttgt acctcaatga gttgggatcc tatacaatag
     4321 tgacctccaa aacacaagac cataatacct gcccgggatg ggcctctgga aacgcataga
     4381 cggccctgac cgcccggctg agggagggtc gggagagact cagccgcgcc ttccaccggg
     4441 aggcggaggt gtgtgcccct tcctccctat acctacctgg ttttggggtg tatgcgggga
     4501 agaaggcctt taatcgaggg ggtttttctt cttcgcgagc gttgggtcct tcagcgcctt
     4561 ctcgatctcg gagaagacgc tcctcttgtc gggagaaccc ttgagagcgg cctcgaggtc
     4621 tctcatgcgc gtctcctgcc gcgccttgaa tctcgatatc tcgtcctcga gcgcgggcgc
     4681 ctcggcggac ctcgggaggt tttcgatcct cgtgtgatcc cccgtcgtga accgctcctg
     4741 gcccccgctc gctatcacgc ggacgatgcc ccgctcctcg agcctcctgg agaggagcga
     4801 gagctcttcc tccgagaccc cgaggatccc cgagaggtcc cccagggagg gcggccgacc
     4861 gtgcacgtgc tcgtggaccc tgacggccgc cacgaacagg tgggcctggg agtacaggtc
     4921 tcgcggtaac ataatggtca ctctagtcca aaggccgcga cgatacaagg catgattcaa
     4981 gaatgacctt gacatatccg atcaagaaac aaatgatgtg ctcgtccgtt cagatgatcg
     5041 caaaaggagg atccgccttg agaaagccga gccttcccat cgttttcctg gcagtcgttg
     5101 tgttcctggc cttcaggggc acctccgacc tgagggcgga tgaaaccacc cataccgtgg
     5161 tgaaggggga caccctgtgg gacatctcca tgaagtacct caagacgccg tggaagtggc
     5221 ctctcgtctg ggcgaacaac caggacatca cgaaccccca cctcatatac cccggggaca
     5281 gggtgatcat cacgaccgag ggcggcagga cgatcctgaa gatcatcccc tcgcctgaac
     5341 gagggggaga agagcgcgag gccgtcttct acacaccccg ggagatcgcc tcagtcaagg
     5401 acaagtcggt agtgctggcc ccccagttcg ccttgtacat gttcagcccc aacctgctca
     5461 aaggctccgg cgagatcgtc aggaaggtcg aaaacggcga gctcatctcg gagaacgacc
     5521 acatcgtcat cagttcgggc tccgccttca agaaagacca ggtcgtcacc atcgtgacca
     5581 agatccagga catcgttgac gaggaaggcg attctgcagg gtacctctac cgcatcacgg
     5641 ggatggcgcg ggttgacgag gtcgtgggcg gcctcgtcaa ggccaccgtc acgtactctc
     5701 tccaggaatc gcggatcggc agcatcatct tcgacgacat cccgcccata aaacccgtgg
     5761 ccctcgacat atccgaacca tcgggcatct ccggggtggt catggatcac cacggcgggg
     5821 tcgtgggcgg ggccaccttc gacctcgtct tcgtgaacgc cggcacgagg cagggggtgg
     5881 aaaagggagc cctcctgtcg gtctacagga agaccgacgc cctgggcggt gatgcaaaca
     5941 ggcaagccgc gtcgttccat gatcccatag gcgtcatcgt ggtgctccag tccctggact
     6001 cgtcttccat ggggctcgtg gttcaatcca aggagctcat cgagaagggt gcgctggttc
     6061 tcggccgaaa gtgagtatcg gcccagggta tgcgacgagg gcccaggggt tgagacggtc
     6121 tccaccctca gcgggaataa gccccggaac tcgatgaacc tcgcagcccg cgcaaggaag
     6181 aacgtcccct ccccatgggg ggcgagcagg atcgggacgt agggctcgat caacctcgtc
     6241 gcccggggtc gacccggtcc tcacacccgc gatggatacc ccggcacggc cgcgaaggga
     6301 acggcgtttg cccgggcgtg caagcggccc ctcagaaggg cgcgccgtga taggtgagga
     6361 tattcatgag ccccagccta tccacccgca cgaaggtgac ccccacacca tcgggcgtct
     6421 tgtgggccac cctccccctc acggagatga ccttgccgaa ctccctgtcg tctatgctgg
     6481 cgtccacgaa gcccccgagc gccacaccgc ggcccgtctc gaggtacatg ccccccttgc
     6541 cgagattcag caccctgcct gtacgcagga cgaacccgtc gtccttgtag gcagcccgca
     6601 agtccacctt caccctcttg tgcgccctgc ggtccttcat ctcatcccac cgcctctctc
     6661 gaaagattac cccactcttc gattcgtgac aatatctccg cgcaggcccc cgatgtccac
     6721 cgtggacaga cccggatgca gggcgaacgt gaagggtccc agacgacgcc cggcccagga
     6781 aggcgtgcat tccacggtat ccggtcccgg ttcgcggaga cccgacgacg tttgcccccc
     6841 tccggccgta cccctccatc ccacggcaac cagcaagtgc aagacgcacc aagcggcgat
     6901 gtcttgcctc acggcccgcc caaccgagga aagtgggcgc ctgtaaaggc agtgtcgttg
     6961 acaccccccc gtcgatacgt tatagatcat ggcatgagga agctctacga cgccctcgcc
     7021 atatcccgga aggtgtctca gctcggcgaa gagatatccc gctcctacat gggcaaagag
     7081 cttctcgtcg tggggatcct caagggcggt ttcatgttca tgtccgacct ggtacgcgcc
     7141 gtctcgttgc ccacgaggat cgatttcgcg cgcctgtcca gctacggcaa gtccgacagc
     7201 cccgaggggg acgtgaagat cgtggacgac gtccacgaag acatacaggg cgtccacgtg
     7261 ctcgtggtag acgacatcat ggacacgggc cagagcatca cagccttcaa ggaacacctg
     7321 gaatccaagg ggcctgcctc ggtgaagatc tgcaccatga tcaacaagaa gtttcgcagg
     7381 tccaaggata tcgacccgga ttactccggc t
//
LOCUS       DAOSCR010000042         7407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6444_length_7407_cov_3.129625, whole genome shotgun sequence.
ACCESSION   DAOSCR010000042 DAOSCR010000000
VERSION     DAOSCR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6444_length_7407_cov_3.129625"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1075)
                     /locus_tag="PLT69_02020"
     CDS             complement(128..1075)
                     /locus_tag="PLT69_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ13257.1"
                     /translation="MHDMSERAVTISVVIPVYQAEACLHELHERLTSVLRTLGPDYEI
                     VLVEDRGQDRSWEVIREIAGKDPRVKAARLSRNFGQHHAIAAGLDMCKGEWVVIMDCD
                     LQDRPEEIPRLYEKALSGHDIVLARRAERQDGVVKRLFSKTFYAVLSYLTETRMDPSI
                     GSFRIMSRKVVRELSKMHEQTRFFGGLVNWLGFSSTTVDVEHAPRMHGKTSYNLKKLV
                     RLAVGAILAFSDKPLRLAVQLGFLISFLSMCFGVYAVITKLMHPSVQLGWTSLIVSIY
                     FMGGLVIGVIGICGLYIGKIFEEVKRRPLYVIDEKINAD"
     gene            1275..2426
                     /gene="rffA"
                     /locus_tag="PLT69_02025"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             1275..2426
                     /gene="rffA"
                     /locus_tag="PLT69_02025"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOJ13258.1"
                     /translation="MHGFDMDIPFNKPFVAGRELYNIAQAVVRGQLAGDGYFTRRCHG
                     WLQEKLGCTKALLTHSCTAAIEMASILADIGPGDEVIMPSFTFVSTANAFVLRGGVPV
                     FIDIRPDTLNMDETLVEQAVTRRTKAVVPVHYAGVPCEMNTICDVARRHGLLVIEDAA
                     QALLSQYEGKYLGTLGTFGCLSFHETKNIISGEGGALLVNDERFVKRAEIIREKGTDR
                     AQFFRGEVDKYTWVDIGSSYLPSELVSAFLFAQMENAERIVAKRRFLFDLYYNALRPL
                     QDRGCARLPYLGKGMAGNGHIFYLITASLEERTRLIGRLKERGILAVFHYVPLHSSPA
                     GRKYGRVSSTMRVTEETSERLVRLPLYFDMGEKDVQRVAHEVMAFYGLA"
     gene            2502..4862
                     /locus_tag="PLT69_02030"
     CDS             2502..4862
                     /locus_tag="PLT69_02030"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ13259.1"
                     /translation="MGEAPRLSFPARHPVLACGLLGLVLVGAFLEARALPLGGSFIGG
                     IDVKDHHYWCIRFFKDHVLSGTFPLWNPHMYSGHPFAANPANFVFYPPIALYVIFPLA
                     WAFNIDLVLHLLACWVGMYLLARRMTGSSAGALTAALVYTFNGQLFERIYGGHLTFIH
                     SAAVIPFVFLAMERVFEEDRPGLFALAGALLGLLILTGNTQIIFYVCVLLCLYLPVRY
                     VSRFGFSGLKRFAAYCLWFLLVPAVSFGVSAVFLLPVAEFIALSDRAEKTYEFAVNGS
                     FGGSLLYTLLVPHPETRKIATFWELGGYCGVIALALAGIGVFSARHRRKAWVVALLLA
                     VTMTFVLGGYTPLYRLYYEFIPLLGNFRVPARALTIAVFFLSLLVSFGASYLWEERDG
                     KKIALLASCSGLVLAALLAFGPLFYDVPLLAPSMTHAYAFVFLGSAAVASLALRKWKV
                     PALALVLFFMFADLFLVYAPGIPVINEAELTRDEDFEKVIAGPAGDYRVMVPISLNPE
                     VPLSPLASRCNVKGYENAGGYVQAAVMDYYRFVHAMAGVEPPVMSRHTLGMDVFTEGR
                     VFSSKILGVKYAVAMTGQGWRLLRADWYMPRARLVHKVKVVPDYRDHLAILKDPGFDP
                     LDVVLLEKADPGHLGEIAQAGKDAGPADSVRMVSYGPDRMEIEASSPLASYLLLSELY
                     YPGWKAYVDGKRAPVLRADYLLRAVPLRAGTHAVTMVYRPASFMAGLAVTLVTLVALA
                     ACLLWAKRRPSAEPEAAAPAGLQGGDDGAAPREGGPQGRRRGGKRR"
     gene            4859..7309
                     /locus_tag="PLT69_02035"
     CDS             4859..7309
                     /locus_tag="PLT69_02035"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ13260.1"
                     /translation="MKSGKKGIGEKGAGRAVKERATRPEVQGGPSRAPNEGSPEGTHP
                     DAVPALPSGAMAFWGRAWPVVLVVTLMLAFFGVKVIPRGDAFIAGIDVRAYFYWFETF
                     TRESIRHGQVPLWNPHYYCGHPFLANPQTFVFYPSTLLFVTLPLPWAFNLDVVLHVLV
                     ASLGAYALTMRLTSNGAAAAFSALSYGMCGYFMDTIYAGHLTMIHTAALIPWVLLALE
                     KALDSGRWRHYALAGLVFGIQILGGEPQNSFYTLFFTAVYVVVRGFPGVRKSSARPAL
                     GLAARFAAFMAVAFSVAAVQVIPSVEFLGLCDRAEKSYTFATGFSYPPQCLFTFLVPK
                     PQLTPIEWGEAHIPFRQLILSWELSGYLGVAGVVLAALGAAMSRGRKGRAALLAVMGL
                     SLVVMLGSYTPLYRVLYGLVPGFATFRIPSRAVVFLVLTLAVFAGMGVDSLMDRTRSR
                     TAGAVVTGAWAVILVLLVAGSLVFTVPILSRQVLVSFAFLCAACGLCVWTLRSGWKAP
                     VYGLVLLVFVDLASTYASAIPLHREKTLLEPNEIERFLAQDKDFTPHAFRVAYPANLD
                     VEYSPLRGMAKGYQNLNGYNPIVLRDFYRFVHDMAAVPLMTVTRHTLALGVFLGDRVF
                     SSRILGVKYAVCLEENGGILAKEAPVYQPRAVLVHDVVYAGGRDEALAHLKDPRFDPA
                     RSVVLEEKDRLIAGVPDAPAKGGQGASGATVVRYEPQRIEVRTQSPTNSLLILSELFY
                     PGWRAYVDGREAPVVKADLILRGVPVPAGSHVVSMVYRPLSFYAGLAVSMAALVVLAA
                     LYLGRKVLSAAAADRHRA"
BASE COUNT         1208 a         2359 c         2366 g         1474 t
ORIGIN      
        1 gtacctgaag ccgggcgagt cctcgtagag gttcgtgttc acgacgaaca gccccatgtc
       61 cttggccttc ctgatgagcg ggacctgcca ttctccgcct gccactatca tgattctctt
      121 ggtcgagtca gtcggcattg atcttctcgt ctatcacgta aagcggtcgc ctcttcacct
      181 cttcgaagat cttccctatg tacagcccgc atatgccgat tacgcctatc acgagaccgc
      241 ccatgaagta tatggaaaca atgaggctcg tccagccgag ctgtacagac gggtgcatca
      301 gcttggtaat gaccgcatat accccgaagc acatggacaa aaaggaaatg aggaaaccca
      361 gctggacggc aagcctcaac ggcttgtcgg aaaaggcgag aatggccccg accgccaggc
      421 gtacgagctt tttcaggttg taggaggtct ttccgtgcat cctgggcgcg tgctccacgt
      481 ccaccgtggt cgaactgaat ccgagccagt tgacgagacc gccgaagaac ctggtctgct
      541 cgtgcatttt cgacagctca cgtacgacct tgcgggacat gatgcggaac gaaccgatgc
      601 tgggatccat tcttgtttcc gtcaggtatg agagtacagc atagaaggtt ttggaaaaaa
      661 gccgtttgac aaccccatcc tgcctttccg ctcttcgcgc cagcacgatg tcgtggcctg
      721 agagtgcctt ttcgtagagc cgtgggatct cttcgggcct gtcctggagg tcgcagtcca
      781 tgatcaccac ccactcgcct ttgcacatgt ccaacccggc ggcgatcgca tggtgctgtc
      841 cgaagttcct gctgagcctg gcggccttca ccctcgggtc tttcccggcg atttcccgga
      901 tgacctccca ggagcggtcc tggcccctgt cttcgacgag aacgatctcg taatccggcc
      961 ccagtgttct caatacactg gtgagccgct cgtgcagttc atgcaagcat gcctcggcct
     1021 ggtagacggg tatcaccacc gatatcgtaa ctgcccgttc actcatgtca tgcatgatgc
     1081 accccaaccg gggatgtcca tgataccatt tggcaagatt cgcattcgat tctatcacag
     1141 catgtcgaag cgcgtaaccc ctcaaagaga aagtcaagct cttccctgcg agggaacgta
     1201 ctgtgaacgg tgctcatcgg acttgcacga tgcggggttt gcgaggtaat gataggtgaa
     1261 gcgattccgg caggatgcat gggtttgata tggacattcc gttcaacaag ccgttcgtgg
     1321 cgggcaggga gctgtacaac atagcccagg cggtggtcag gggccagctc gcgggcgacg
     1381 gctacttcac gaggcgatgc cacgggtggc tgcaggagaa gctcggctgc accaaggccc
     1441 tgctcacgca ctcctgcacg gcggcgatcg agatggcctc catcctcgcg gacatcggcc
     1501 ccggcgacga ggtgatcatg ccctcgttca ccttcgtgtc caccgcgaac gccttcgtgc
     1561 tcaggggagg ggtgcctgtc ttcatagaca tcaggcccga caccctgaac atggacgaga
     1621 ccctcgtcga acaggccgtg acacggcgca ccaaggctgt cgtgcccgtg cactacgcag
     1681 gcgtcccctg cgagatgaac acgatctgcg acgtggcgcg aaggcacggc ctcctcgtga
     1741 tcgaggacgc agcccaggcg ctcctctccc agtacgaggg gaagtacctc ggcacgctgg
     1801 gcaccttcgg ctgcctgagc ttccacgaga cgaagaacat cataagcgga gagggtgggg
     1861 ccctgctcgt aaacgacgag aggttcgtca agcgcgccga gatcatccgt gagaagggca
     1921 cggacagggc gcagttcttc aggggcgagg tggacaagta cacctgggtg gacatcgggt
     1981 cgtcctacct gccgagcgag ctcgtgagcg ccttcctctt cgcccagatg gagaacgcgg
     2041 agcgcatcgt ggccaagagg cgtttcctgt tcgacctcta ctacaacgcc ctgaggccgc
     2101 tccaggacag ggggtgcgcg cgtctgccct acctcggcaa aggcatggcc ggaaacggcc
     2161 acatcttcta cctgatcacg gcatccctcg aggagcggac gaggctcatc ggccgtctga
     2221 aggaaagggg tatcctcgcc gtgttccact acgtgccgct gcactcgtcg ccggcgggca
     2281 gaaagtacgg cagggtatcg tccaccatgc gcgtgacgga ggagacgagc gagcgcctgg
     2341 tgcgcctgcc gctctacttc gacatggggg agaaggacgt gcagcgggtg gcgcacgagg
     2401 taatggcatt ctacggtctc gcgtgaaaga ttccgcgtgc gggacgaagg cgcgcaaggg
     2461 ggaggggatg ctggcggctg ctcggtgcat gggtgaaggg gatgggtgag gccccacggt
     2521 tgtcctttcc cgcgcggcac ccggtccttg cgtgcggcct tttgggcctt gtgctggtcg
     2581 gcgccttcct ggaggccagg gcgctgcccc tgggcggttc cttcataggc ggcatcgacg
     2641 tcaaggacca ccactactgg tgcatccgtt tcttcaagga tcacgtgctg tcaggcacct
     2701 ttcccctgtg gaacccccac atgtactctg gccacccgtt cgccgcgaac ccggcgaact
     2761 tcgtcttcta cccgccgatc gccctgtacg tgatcttccc ccttgcttgg gccttcaaca
     2821 tcgacctcgt gctgcacctg ctcgcgtgct gggtggggat gtacctgctg gcgcgccgca
     2881 tgacgggctc ttcggccggg gcgctcacgg ccgccctcgt gtacacgttc aacggccagc
     2941 tgttcgaaag gatatacgga ggccacctca ccttcatcca ttccgcggcc gttatcccct
     3001 ttgtcttcct cgccatggag cgggtgttcg aggaggacag gcccggcctg ttcgcccttg
     3061 cgggcgccct cctgggcctg ctgatcctga ccgggaacac ccagatcatc ttctacgtgt
     3121 gcgtgctgct gtgtctctac ctgcctgtgc gctacgtgtc gcgcttcggc ttttccggcc
     3181 tgaagcgctt tgcggcttac tgcctttggt ttctcctcgt gcccgccgtg tcgttcggcg
     3241 tgtccgccgt attcctgctg cctgtggccg agttcatcgc cctgtccgac agggcggaga
     3301 agacctacga gttcgccgtg aacgggtcgt tcgggggttc actcctgtac accctcctgg
     3361 tcccgcaccc ggagacgcgc aagatcgcca cgttctggga gttgggcggg tactgcgggg
     3421 tcatcgccct cgccctcgcg ggcattgggg tcttttcggc aagacacagg aggaaggcat
     3481 gggtcgtcgc cctgctgctc gcggtgacga tgaccttcgt gttgggtggg tacaccccct
     3541 tgtaccggct ctactacgag ttcatcccgc tcctgggcaa tttcagggtg cctgcgcggg
     3601 cgctcacgat agcggtgttc ttcctttccc tgctcgtgtc cttcggcgct tcgtacctgt
     3661 gggaagagcg cgacgggaag aagatcgcgc tcctcgcctc ctgttccggg ctcgtcctcg
     3721 ccgcgctgct tgccttcggg ccgctcttct acgacgtccc gctgctggcg ccttccatga
     3781 cccacgccta cgcgttcgtc tttctgggct cggcggcagt ggcctctctc gcgctgagga
     3841 agtggaaggt cccggccctc gctctggttc tcttcttcat gttcgcggac ctcttcctgg
     3901 tgtatgcgcc agggataccg gtcatcaacg aggccgagct cacccgggac gaagacttcg
     3961 agaaggtcat cgccggccct gcgggcgact accgcgtcat ggtgcccatc tcgttgaatc
     4021 ccgaagtgcc gctttcaccc ctcgcgtcac gctgcaacgt gaaggggtac gagaacgcgg
     4081 gcgggtacgt gcaggcagcg gtcatggact actaccggtt cgtccatgcc atggccgggg
     4141 tggagccgcc cgtgatgtcc aggcacacgc tcggcatgga cgtcttcacc gagggccgcg
     4201 tcttcagctc gaagatcctc ggggtcaagt acgccgtcgc catgacaggc caggggtggc
     4261 gactcctgcg ggcggactgg tacatgccga gggcccgtct cgtccacaag gtgaaggtgg
     4321 tgcctgacta tcgagaccac ctcgccatac tcaaagaccc ggggttcgac cccctggatg
     4381 tcgtgctcct cgagaaggcg gaccccggtc acctggggga gatcgctcag gccggaaaag
     4441 atgcggggcc tgcggactcg gtgaggatgg tctcctacgg gcctgacagg atggagatcg
     4501 aggcctcatc ccctttggcg tcataccttc tcttgagcga gctgtactac cccgggtgga
     4561 aggcatacgt cgacgggaag cgcgcgcccg tcctcagggc cgactacctg ctcagggcgg
     4621 taccgctgcg tgcaggcacg cacgcggtga ccatggtcta caggcctgcg agcttcatgg
     4681 ccggcctcgc cgtaacgctc gtgacgctcg tcgccctcgc tgcatgcctg ctgtgggcga
     4741 agaggaggcc ctcggcggag cctgaagcgg ccgcgccagc cggcctgcag ggtggcgatg
     4801 acggtgcggc cccaagggaa ggcgggcctc agggaagacg gcgtgggggg aaaaggcggt
     4861 gaagtcgggg aagaagggta tcggcgagaa gggggcgggc agggccgtca aggagcgggc
     4921 gacaaggcct gaggtgcaag ggggcccgtc tcgcgcaccg aacgagggaa gcccagaggg
     4981 gacgcacccg gacgcggtgc cggcattgcc ttcgggcgcc atggcctttt ggggcagggc
     5041 gtggccggtg gtgctcgtcg tgacgttgat gctcgcgttc ttcggcgtaa aggtgatccc
     5101 gcgcggggac gccttcatcg caggcatcga cgtgagggcc tacttctact ggttcgagac
     5161 gttcaccagg gagagcatcc gccacgggca ggtcccgttg tggaacccgc actactactg
     5221 cggtcacccc ttcctggcca acccgcagac cttcgtgttc tacccctcca cgctcctgtt
     5281 cgtgaccctt cccctcccgt gggcgttcaa cctggatgtc gtgctccacg tgctcgtggc
     5341 ctccctcggc gcgtacgcgc tcacaatgcg cctcacgtcg aacggggctg cggcggcatt
     5401 ctccgccctg tcgtacggca tgtgcggcta cttcatggac acgatctacg ccgggcacct
     5461 caccatgatc cacaccgcag cgctcatccc gtgggttttg ctcgcgctcg agaaggccct
     5521 cgactcgggg cggtggcggc attacgcgct cgcggggctc gtcttcggca tccagatcct
     5581 cggcggagag cctcagaact cgttctacac cctgtttttc accgcggtgt acgtcgtggt
     5641 gagggggttt cccggggtcc ggaagtcatc ggcaagaccc gccctcggcc ttgccgcgag
     5701 gttcgctgcc ttcatggccg tggcgttctc ggtggctgcg gtgcaggtca tcccttcggt
     5761 cgagttcctc ggcctgtgcg acagggccga gaagtcgtac acgttcgcca ccgggttctc
     5821 gtacccgccc cagtgcctct tcaccttcct cgtgccaaag ccccagctga ccccgatcga
     5881 gtggggcgag gcccatatcc ccttccggca actcattctc agctgggagc tctccgggta
     5941 cctgggcgtg gccggcgtcg tgctggctgc cctgggcgcg gccatgtcac gtggcaggaa
     6001 ggggagggcc gcgctgcttg cggtcatggg gctctcgctg gtcgtcatgc tcggcagcta
     6061 tacgcccctg taccgcgtgc tctacggcct cgtgcccggg ttcgcaacgt tccggatacc
     6121 ttcgagggcc gtggtgttcc tggtcctcac cctcgcggtc ttcgcaggca tgggggtcga
     6181 ctcgctcatg gacaggacga ggtcgcgtac cgcaggggcg gtcgtcacgg gcgcgtgggc
     6241 ggtgatcctc gtgctcctcg tcgcggggtc gctggtcttt accgtcccca tcctctcgcg
     6301 ccaggtcctt gtgtctttcg cctttctctg cgcagcctgc ggcctctgcg tctggaccct
     6361 gaggtcgggc tggaaggccc ctgtctacgg gctcgtgctc ctggtcttcg tggatctcgc
     6421 ttcgacctac gcctcggcta tccccctgca cagagaaaag acgctgctcg agccgaacga
     6481 gatcgagagg ttcctcgcgc aggacaagga tttcacgcca catgccttcc gcgtcgccta
     6541 cccggcgaac ctcgacgtcg agtacagccc gttgaggggc atggccaagg gataccagaa
     6601 cctgaacggg tacaacccca tcgtgctcag ggacttctac cgtttcgtgc acgacatggc
     6661 cgccgtgcct ctcatgacag tgacgaggca cacgctggcg ctcggggtgt tcttggggga
     6721 cagggtcttc tcttcgagga tcctgggggt caaatacgcg gtgtgcctag aagagaacgg
     6781 cggaatctta gcgaaggaag cccccgttta ccagccgagg gcggtgctcg tgcatgacgt
     6841 ggtatatgca ggcggccgtg atgaagccct ggcgcacctc aaagacccgc gcttcgaccc
     6901 ggcgcgctcg gtcgtgctcg aggagaagga ccgcctgata gcgggggtgc cggatgctcc
     6961 cgccaagggg ggacagggcg catcgggtgc gaccgtcgtg cgctacgagc cacagcgcat
     7021 cgaggtgagg acacaaagcc cgacgaactc cctcctcatt ctgagcgagc tcttctaccc
     7081 gggctggagg gcgtacgtgg acggcaggga ggcgcctgtg gtgaaggcgg acctcatcct
     7141 caggggcgta cccgtgcccg caggctccca cgtggtgagc atggtgtacc ggccgctgtc
     7201 tttctacgcg gggctcgcgg tcagcatggc cgccctggtc gtcctggcgg ccctgtacct
     7261 cgggaggaag gtgctctccg cagccgcggc ggatcggcat cgagcctgag cgagttcaag
     7321 gatcccgaag cgcccgggga ttgttactat acccgcaaca acatgacccg aaaggcccag
     7381 gggtcccgac gcacacgggg attgtgc
//
LOCUS       DAOSCR010000043         7395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6458_length_7395_cov_3.633243, whole genome shotgun sequence.
ACCESSION   DAOSCR010000043 DAOSCR010000000
VERSION     DAOSCR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_7395_cov_3.633243"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLT69_02040"
     CDS             complement(<1..738)
                     /locus_tag="PLT69_02040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ13261.1"
                     /translation="MRLKRDLRYVVEKGDTSKVVSFRDYQGNEITCRIKDYSRTGVSF
                     RLDDGSLILHIGDVIHELRFLSAGTEVHCADATVIHIEDEEEDGAVVSTIGCSYNDNP
                     MDVYSVVRADKITKLQNDFLDFVQSMAIEDNLDPEFVNLTAQIHYLLEGFSDRLKRDE
                     EAISREEEELREALTDTLKELAFDALFDELNKFYDRFSAVTGRFTDPKQHFIHREFFQ
                     KRLNEYLSRSALFNRSITKPLGYAGDFE"
     gene            complement(974..1462)
                     /locus_tag="PLT69_02045"
     CDS             complement(974..1462)
                     /locus_tag="PLT69_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOJ13262.1"
                     /translation="MKRAGVLTLSDKGASGLRRDESGPVVCGALTAMGIAVESYEVIP
                     DDMETIVNRLKALCDELGLDLAVTTGGTGLTPRDVTPEATLAVIHRRVPGMEEAMRME
                     SMKKTPHAMISRAVVGTRDRTLIVNLPGSPKAARECLEVIAPALGHALDKLGGDTSDC
                     GA"
     gene            complement(1459..1896)
                     /locus_tag="PLT69_02050"
     CDS             complement(1459..1896)
                     /locus_tag="PLT69_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13263.1"
                     /translation="MESNGDEFTTRSLFQRIQAMSVAEKIDLAHKAGKDARTILIRDS
                     NKLVQLAVVQSPKITESEILAIAANRQVSDEVLKEIGMSKEWLKNYQVRLALVNNPKT
                     PLSIALAQIPYLNKRDLGLLAKNRNVPRPVVVAAEGRLREVKR"
     gene            complement(1886..3970)
                     /gene="fusA"
                     /locus_tag="PLT69_02055"
     CDS             complement(1886..3970)
                     /gene="fusA"
                     /locus_tag="PLT69_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ13264.1"
                     /translation="MRKFRTENLRDVIIMSHSGSGKTSLGEAMLYNAKATTRLGKVLE
                     GNSVLDFEPEEIKRQSSIFSALHNFKWKNAGVIMLDTPGDTNFTSEVILGLKSADNAL
                     FVIDAVDSIKPYTENLWRMAVSEGLTRTCFVNKMDRERANFDQVLSDISEILETKPLP
                     LTLPIGAEDSFSGVVDVLKKKAYLYEKDGSGSFKEAPVPQDMQATLEEVYGKVVEDLA
                     EVDDALIEKYLEGGDLSSAELENALVEGLARGLFLPVFCGSALLNMGVQPLMDFMVTS
                     FISPDRRPIPPLKNEKGETKEYKPSEDQPFSGYVFKTMSDPYTGTLSIIRCLSGTLNP
                     DSTVYNSKRDVKERVGSLFMLEGKAQKPLEQVGPGDIFAVAKLKETKTGDTLLSENIP
                     FTLPAPVFPEPIMNMAVTPKTKADVDKIMPAFQKIMDEDPILKVFRDDQTGEFILSGL
                     GQLHIEIAVDRLKRRFGVEVDLKTPKVPYKETITAMAEVQGKHKKQTGGHGQYGDCWL
                     RVEPLERGKGFEFVNAIVGGVIPKQYIPAIEKGVLEAMQSGPLTGNPVVDVKVTVYFG
                     SYHEVDSSEMAFKIAGSLAFKKAMEVCKPILLEPIMNLTVTIPEECMGDVMGDLNSRR
                     AKIEGMTARGKYQELKAQVPLAEVLTYAANLTSITSGRGTFTMSFSHLDPVPYNLQEK
                     IIEEAKAARDGK"
     gene            4320..5243
                     /gene="holB"
                     /locus_tag="PLT69_02060"
     CDS             4320..5243
                     /gene="holB"
                     /locus_tag="PLT69_02060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOJ13265.1"
                     /translation="MNAVWKDIQGDEGVKALLREYCLTGRVPHALLFAGEAGAGKTRM
                     ALEFFKALNCQEHAAEACGRCPSCVKADSGSHPDIHVVRPEEGKRWIHVEKVRETIQE
                     ATLRPFEARTRCILVEPAEALNVQSANALLKTLEEPPGNTVIVLVSHRPSLILPTVAS
                     RCRTLRFHGAKQPPRVQEEEGGEAPSSLYGLVDEEVRRDVIGLLFGADPALLARKYSE
                     QADVDLLDDVLTVSEAIARDVLVSRTAPGRVLNRELLDMGLGRCGLERVEEILEVVSA
                     IRRGVNENVNVRVGATELFARISRLSGLAGG"
     gene            complement(5256..5783)
                     /locus_tag="PLT69_02065"
     CDS             complement(5256..5783)
                     /locus_tag="PLT69_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOJ13266.1"
                     /translation="MSHRDTVEPEPLTTSEVVKRVRENKLRQDMEVIQALSALVRGTS
                     RFDVTCTGEYLEGHVTTLDPRVMKRLKTGDYAVQAHLDLHGALREEAKVMLRAFVENS
                     HAMGLRCLLVIHGRGLKSESGPVLKEAVVKWLTTGELSRLVLAFCSARPCDGGTGAIY
                     VLLRRRPERARWKRP"
     gene            6148..7059
                     /locus_tag="PLT69_02070"
     CDS             6148..7059
                     /locus_tag="PLT69_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ13267.1"
                     /translation="MNIHQVHIFNVAAKTLSITKTAKKMNLSQPSVSIQIKDLEDSLG
                     VRLFDRINRRITLTDAGKVFLDYSERLLRLIEEIEASMKGFSSGDAGRVVIGASSTVG
                     VYVLPRFLGEFKERYPKAEISLNIMNRKEAVEQCLAGSLDFAFMQDPPRHADLDVEFF
                     MKDELLIVCSPRHRWAQNEFLTMKTLSTEPEPIILREEGSGTRALIEYVLKRYGIERK
                     VTMELSSSEGIKRAVEANLGVAVLSKSVVSKEVDAGTLVALPIRDLDTVREFYIVRNR
                     KRASLPLVERFGEFILSCREKIAPIPG"
BASE COUNT         1261 a         2287 c         2359 g         1488 t
ORIGIN      
        1 ctcgaagtcc ccggcgtagc cgaggggctt cgtgatgctc ctgttgaaca gggcggaccg
       61 cgacaggtac tcgttcaggc gcttctggaa gaactcgcgg tggatgaagt gctgcttggg
      121 gtccgtgaag cggccggtca cggcgctgaa acggtcgtag aacttgttga gctcatcgaa
      181 gagggcgtcg aaggcgagct ccttcagcgt gtccgtgagc gcctccctca gttcctcttc
      241 ctccctcgat atggcctctt cgtcccgctt gagccggtcg gagaatccct ccagcaagta
      301 gtggatctgc gccgtgaggt tcacgaactc cgggtccagg ttgtcctcga tggccatgga
      361 ctggacgaag tcgaggaagt cgttctggag cttggtgatc ttgtcggcgc ggacgaccga
      421 gtagacgtcc atggggttgt cgttgtacga gcagccgatc gtggatacaa cggcgccgtc
      481 ttcctcttcg tcctcgatgt ggatgaccgt ggcgtccgca cagtggacct ccgtgccggc
      541 cgagaggaac ctgagctcgt ggatgacgtc gcctatgtgg aggatgaggc tgccgtcgtc
      601 gaggcggaac gagacgcctg tccgcgagta gtccttaatc ctgcaggtga tctcgtttcc
      661 ctggtaatcc ctgaaggaca cgaccttgct tgtgtcaccc ttttcgacca cgtaccgaag
      721 atcacgcttg agtctcaagc agcgttcctc cctccatctc aaacccgagc gggcttgtta
      781 catccccggt tgagggccct ctcggtggac tcaacggcaa cctctcgata aaactttatt
      841 cccgaccgtg atccctgggc atcggccctg tgttcctgaa ctcccccgcc accgggaaag
      901 acgacgcgaa tccagcctgc acccgctcgc ggggccggcc actgcttcat gacgcctgcg
      961 ggccgtccgc gcttcaggcg ccgcaatcgc tcgtgtcgcc tcccagcttg tcgagggcgt
     1021 gcccgagcgc aggggcgatg acctcgagac actctcgcgc cgccttcggg ctcccaggca
     1081 ggttcacgat cagggtgcgg tctctcgtcc ccaccacggc acgcgagatc atggcgtggg
     1141 gggtcttctt catgctctcc atccgcatcg cctcttccat gccgggcact cgcctgtgta
     1201 tcacggcaag cgtggcctcc ggcgtgacgt ccctgggcgt gagccccgtg cccccggtcg
     1261 tgaccgcgag atcgaggccg agctcgtcgc acaaggcctt cagccggttc acgatggtct
     1321 ccatgtcatc gggtatgacc tcgtaggact cgaccgcaat ccccatggcc gtgagcgccc
     1381 cgcacaccac gggcccgctc tcgtcccgtc tcagccccga cgcccccttg tcgctcaggg
     1441 tgaggacgcc ggccctcttc atctcttgac ctccctgagc ctcccctcgg ccgccaccac
     1501 gaccggcctc gggacgttcc tgttctttgc aagcagcccg agatcgcgct tgttgaggta
     1561 ggggatctgg gccagggcta tggaaagcgg ggtcttgggg ttgttcacga gggcgagcct
     1621 cacctggtag ttcttgagcc actccttgct catcccgatt tccttgagaa cctcgtctga
     1681 gacctgccgg ttcgcggcga tggccaggat ctcgctctcg gtgatcttcg gcgactgcac
     1741 caccgcgagc tggaccagct tgttggaatc ccggatgagt atggtcctgg catccttgcc
     1801 ggccttgtgc gcgagatcga tcttctcggc gacgctcatg gcctggattc tctggaacag
     1861 gctcctcgtg gtgaactcat caccgttact ttccatcccg agccgctttg gcctcctcta
     1921 tgatcttttc ctggaggttg taaggcactg ggtcgaggtg gctgaagctc atggtgaacg
     1981 ttcccctgcc cgaggtgatc gacgtgaggt tcgccgcgta ggtgaggacc tcggcgaggg
     2041 ggacctgggc cttgagctcc tggtacttgc ccctcgcggt catgccctcg atcttggccc
     2101 tgcggctgtt gaggtctccc atgacgtcac ccatgcattc ctcggggatc gtgacggtga
     2161 ggttcatgat gggttccagg agaatgggct tgcacacctc catggccttc ttgaaggcca
     2221 gcgagccggc gatcttgaag gccatctccg aggaatcgac ctcgtggtag gaaccgaagt
     2281 acaccgtcac cttgacgtcg accaccgggt ttcccgtgag cggacccgac tgcatggcct
     2341 ccagtacgcc cttctctatg gcgggtatgt actgcttggg aatgacgccg ccgacgatcg
     2401 cgttgacgaa ctcgaaaccc ttgcccctct cgaggggttc caccctgagc cagcagtccc
     2461 cgtactgtcc gtggccgccc gtctgcttct tgtgcttgcc ctggacctcg gccatggcgg
     2521 tgatggtctc cttgtagggg accttcggcg tcttgaggtc gacctcgacc ccgaaacgcc
     2581 tcttgagcct gtcgacggcg atctcgatgt ggagctgccc gaggccggaa aggatgaact
     2641 ctcctgtctg gtcgtcgcgg aagaccttga ggatcgggtc ctcgtccatg atcttctgga
     2701 aggcgggcat gatcttgtcc acgtcggcct tcgtcttcgg ggtaacggcc atgttcatga
     2761 tcggctcggg gaagaccggc gcaggcagcg tgaacgggat gttctccgag agcagggtgt
     2821 cccccgtctt ggtctccttc agcttggcaa cggcgaagat gtcccccggc ccgacctgct
     2881 cgagaggctt ctgcgccttg ccttccagca tgaagaggga acctacgcgt tccttcacgt
     2941 cgcgcttcga gttgtagacg gtcgagtcgg ggttgagcgt gccggacagg caccgtatga
     3001 tgctcagggt gccagtgtac gggtcgctca tggtcttgaa cacgtacccg ctgaacggct
     3061 ggtcctcgga cggcttgtat tccttcgtct cccccttctc gttcttgagc ggggggatgg
     3121 ggcgtctgtc gggcgagatg aacgaggtga ccatgaagtc catgaggggc tgcacgccca
     3181 tgttcagcag tgcgctgccg cagaacaccg gcaggaagag acccctggcg agaccctcga
     3241 cgagcgcgtt ctcgagttcc gcactcgaga ggtcgccgcc ttccaggtac ttctctatga
     3301 gggcgtcgtc cacctcggcg aggtcctcga ccaccttgcc gtagacctcc tcgagggtcg
     3361 cctgcatgtc ctggggcacg ggtgcctcct tgaaggatcc cgagccgtct ttctcgtaga
     3421 ggtacgcctt cttcttgagc acgtccacca cgccggaaaa gctgtcctct gcgccgatgg
     3481 ggagcgtcag gggcaagggt ttcgtctcga ggatctccga gatgtcggac agcacctggt
     3541 cgaagttggc gcgctccctg tccatcttgt tcacgaagca cgtgcgggtg aggccctctg
     3601 aaaccgccat gcgccaaagg ttctcggtat acggctttat ggagtccacc gcgtcgatca
     3661 cgaacagggc gttgtccgcg gacttgaggc cgaggatcac ctcggaggtg aagttggtgt
     3721 cgcccggcgt gtcgagcatg atgacgcccg cgttcttcca cttgaaattg tgcagcgcgc
     3781 tgaagatcga cgactggcgc tttatctcct ccggctcgaa gtcgaggaca gaattgcctt
     3841 cgaggacctt gccgagcctc gttgtggcct tcgcgttgta gagcatggcc tcgcccaggg
     3901 aggtcttccc cgagccggaa tgcgacatga tgatgacatc tctgagattt tcagtgcgga
     3961 acttcctcat cggtcctccc ctcgagccaa gtatcgaatc ttggctttat ccgaaaagcc
     4021 gagatgaagt caaggagcgg cttgttgact cgcggagatc ccccggaacc aggcgtgtgg
     4081 acgtcaagtg ggtgcatcct tgacaagagc gtggacggca gggacggatc cttcggggcg
     4141 ggacgtccga tcggccgagg aaggggcatg aacgggaggg tgctctccgc gggggctcgg
     4201 ggggcggtgc ggtgtgtgtc gaccctccgg tcggccggta gccgttttcg ccttcgtgca
     4261 gtctgcatgc ccatgagagg gcgcccggcc ggttgcacgc tgcgatggct tggcgtagaa
     4321 tgaacgccgt gtggaaggac atccagggcg acgagggcgt gaaggccctc ttgagggagt
     4381 actgcctcac cggccgcgtc ccccacgcgc tcctcttcgc cggggaggcg ggtgcgggaa
     4441 agacgaggat ggcactcgag ttcttcaagg ccctcaactg ccaggaacat gcggccgagg
     4501 cctgcggccg ttgcccctcg tgcgtaaagg ccgactcggg gagccacccg gacatccacg
     4561 tggtgaggcc ggaagagggg aagcggtgga tccacgtcga gaaggtccgg gagacgatcc
     4621 aggaggcgac cctgaggcct ttcgaggcga gaacgaggtg cattctcgtg gaacctgccg
     4681 aggcgctcaa cgtgcagagc gcgaacgccc tgctcaagac cctcgaggag ccgcctggaa
     4741 acaccgtcat cgtgctcgtc agccacaggc cgtccctgat cctgcccacc gtcgcctcgc
     4801 gctgcaggac actgaggttc catggcgcga agcaacctcc ccgcgtgcag gaagaagagg
     4861 gcggggaggc gccgtcttcc ctgtacgggc tcgtcgacga ggaggtgcgt cgagacgtca
     4921 tcgggctcct cttcggggcg gacccggctc tcctcgcgag aaagtactcc gagcaggcgg
     4981 acgtggacct cctggatgac gtcctcaccg tgagcgaggc catcgccagg gacgtgctcg
     5041 tctccaggac ggcgcccggc agggtgctga acagggagct cctggacatg gggcttggcc
     5101 gctgcggtct cgaacgtgtc gaggagatcc tcgaggtcgt ctccgccata cggagagggg
     5161 tcaacgagaa cgtgaacgtc cgggtcgggg ccaccgaact gttcgcgagg atctccaggc
     5221 tgtcgggcct tgcgggcggc tgaggccttt cgtccctagg gtctcttcca tcgcgccctc
     5281 tcggggcgtc tcctgagcag gacgtagatg gcgcccgtgc ccccgtcgca cggccgggcc
     5341 gagcagaacg caaggacgag cctcgacagc tcgcccgtgg tgagccattt caccaccgcc
     5401 tccttgagca cggggcccga ctcagacttg agacccctcc cgtggatcac gagcaggcat
     5461 ctgaggccca tggcgtgact gttctccacg aaggccctga gcatgacctt ggcctcctcc
     5521 ctcagtgcgc cgtgcaggtc gaggtgcgcc tggacggcgt agtcgccggt cttcaggcgt
     5581 ttcatgaccc tcgggtcgag ggtggtcacg tggccctcga ggtactctcc ggtgcaggtc
     5641 acgtcgaacc tcgaggttcc cctgacgagc gcgctgaggg cctggatcac ctccatgtcc
     5701 tgtctcagct tgttctcgcg cacccttttc acgacctccg acgtggtgag aggctcgggc
     5761 tccacggtgt ccctgtgaga catgggcctc acgccctcca tcgcgcgtcg gaacagggtt
     5821 gcatcgtcca tctcctcccg ggtcgcggga aggggttcga cacgggcttc ttgtcgcggt
     5881 gtctctcgtg tcccccgctt gacaacgatg ccctcgagct tggagaaggg tcggttgaag
     5941 ggaggcccgt ccttcatggg ggtggtgtaa caaaaaaaaa gggcccactc aagaagaggt
     6001 ggtagtgggc cgaaaaagga ggtctatagc cctttgttgg gttgtcgttg acgcgtgctg
     6061 tatacatgcg gttgttcatg ctgtcaaata cagatatctt atggtagtgt taaagaatag
     6121 taaattatgg tgtagcaggg aacgctcatg aacatccatc aggtccacat attcaatgtc
     6181 gccgccaaga cgctgagcat cacgaagacg gcgaagaaga tgaacctctc gcagccttcg
     6241 gtgagcatcc agatcaagga cctcgaggac tcgctcggcg tccgcctgtt cgaccgcatc
     6301 aaccggagga tcaccctgac tgacgccggc aaggtgttcc tcgactactc ggaaaggctc
     6361 ctgagactca tcgaggagat agaggcctcg atgaagggct tcagctcggg cgatgccggg
     6421 agggtggtga tcggcgcatc cagcacggtg ggggtctacg tgcttcccag gttcctgggc
     6481 gagttcaagg aacgctaccc gaaggcggag atctcgttga acatcatgaa caggaaagaa
     6541 gccgtcgaac agtgtctcgc cggttccctc gatttcgcct tcatgcagga ccctcccagg
     6601 catgcagacc tcgacgtcga gttcttcatg aaggacgagc tcctgatcgt gtgttccccg
     6661 aggcacaggt gggcccaaaa cgagttcctc accatgaaga ccctctccac ggaacccgag
     6721 ccgatcatcc tcagggagga ggggtccggg actagggccc tcatcgagta tgtcctcaag
     6781 cgctacggca tagaacgcaa agtgaccatg gaactgagtt cttccgaggg catcaagcgg
     6841 gccgtcgagg cgaacctcgg ggtggctgtc ctctccaaga gcgtcgtcag caaggaggtg
     6901 gatgcgggaa ccctcgtggc gcttcccata cgggatctcg acacggtgcg cgagttctac
     6961 atcgtgagga accggaaaag ggcctccttg ccactcgtgg agaggttcgg cgaattcatc
     7021 ctctcgtgca gggagaagat cgcgccgatc ccaggctaga gctgaaccgg ttctttctcc
     7081 cacctcctac ggggttttcg ccgcaccggg cgccgacccc cggcccggac aggttccccg
     7141 gtgttcgttc gtgcctggac acgcccgaac cccaggtcat ggtgtctcga gccccgcatg
     7201 gacgtatgtg ccgctccatg gggaccgggt gggacgacgt gccctcgcag gggccgggac
     7261 gatcgtacgc cccgaggccg aacgcggctg tacgccaaag gccggcttcg acaggacagg
     7321 gaaacgcatg tgcaacgggt atcggctcgc cgtgcgctca aggcgacgag ggcgcgaccg
     7381 ggcccggtat ccaag
//
LOCUS       DAOSCR010000044         7372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6475_length_7372_cov_3.485035, whole genome shotgun sequence.
ACCESSION   DAOSCR010000044 DAOSCR010000000
VERSION     DAOSCR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6475_length_7372_cov_3.485035"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLT69_02075"
     CDS             complement(<1..497)
                     /locus_tag="PLT69_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ13268.1"
                     /translation="MIVIPAVDLYQGKVVRLARGDYNEVTVYSDDPVAVARSFEDAGA
                     GRIHVVDLDAAAQGRGANLDAIGAIARALGIEVELGGGIRTVEDARRAFDLGVKYVIL
                     GTVCVRDPSTASSILEAFPGRVAIGIDALGGRAAITGWKEVTSMTALDLARHYEKLRP
                     AFIVFT"
     gene            complement(494..751)
                     /locus_tag="PLT69_02080"
     CDS             complement(494..751)
                     /locus_tag="PLT69_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13269.1"
                     /translation="MRFSPAQVETMDGARRPGRPLRFTFEGGSVEVRAVRAHWYEAYT
                     DPSFHPDEYFMVESTHETVYLLRYSTLFSSWWVRREGGGGT"
     gene            complement(748..1326)
                     /gene="hisB"
                     /locus_tag="PLT69_02085"
     CDS             complement(748..1326)
                     /gene="hisB"
                     /locus_tag="PLT69_02085"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOJ13270.1"
                     /translation="MREATVTRKTRETDITVKVCLEGGRTDISVPSGFLAHMLESLGV
                     HASMGLVVKASGDVHVDLHHTVEDIGIVLGKAIKDALGDKRGIERFGLAKVPMDEALV
                     EVCIDLSGRPFFAVHGAEAFKGRCGDIDLDLVVEFFRALAFNAGITMHVTVGAHANGH
                     HLAEACFKAFARALKEASAVTGVGVPSTKETL"
     gene            complement(1323..3953)
                     /gene="alaS"
                     /locus_tag="PLT69_02090"
     CDS             complement(1323..3953)
                     /gene="alaS"
                     /locus_tag="PLT69_02090"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOJ13271.1"
                     /translation="MRGSDIRTMFLDYFQDKGHTLVPSSSLVPSDDPTLLFTNAGMVQ
                     FKATFLGTQKRPYSRATSSQKCVRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFK
                     RGAIEYAWELLVDRMGLDPSRLYVSVHHTDDEAYDIWNRTIGVASDRILRLGDKDNFW
                     AMGDTGPCGPCSEIIFDQGPGVGCARPECSPGECDCDRYLEIWNLVFMQFNKDEQGNL
                     TRLPAPNIDTGMGLERITAVLQGVTSNYHTDLFTGLIGFIGELSGVSYGNDGETDVSI
                     RVVADHARACAFLVSDGVLPSNEGRGYVLRRIIRRAARHGKMLGFDRPFLHRVAMRVA
                     SDMSPAYPELAMRSEFVDKVIASEEERFLKTLDRGLGLLDEIVRDLKANGKTLMPGRE
                     VFVLYDTFGFPVDLTADIARKEGIEIDTAGFEAEMEIQREKARGASSFAGTGLESAWS
                     AASGSRFVGYDTLETDSLIVEMRSMDASSELEAAEEGEELLVATAVTPFYGESGGQVG
                     DTGEIVSPSGRARVLDAVKTESDTVVHRVRVVSGRLELGQGVTLRVDAHRRKAVMRHH
                     SATHLLHRALREVLGEHVHQAGSLVDDSRLRFDFTHFSALSPEELAHVENLVNQYVKE
                     DLPITTEILSRDEAVSRGAMALFTEKYGDTVRLVSMGDVSRELCGGTHCSSTGQIGMV
                     KVVSVSSISAGLRRIEAVAGFRCLEHARSLSSLVAGASQSLRCSPEEIEERIKGLLAK
                     IKDQEATIEELTVKLATGTGPSADEREYDAGGLKVVVKKVQQADTAQMRQVGDRIKER
                     ISKGAVLLYTDEGDKATFMIMATKGVEGVMDAGRVMKKAMAAVGGRGGGKELFAQGGA
                     GADSTGKVIEIFLESAGVKP"
     gene            complement(3950..4288)
                     /locus_tag="PLT69_02095"
                     /pseudo
     CDS             complement(3950..4288)
                     /locus_tag="PLT69_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
     gene            complement(4367..5542)
                     /locus_tag="PLT69_02100"
     CDS             complement(4367..5542)
                     /locus_tag="PLT69_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ13272.1"
                     /translation="MDINPILRHAVEITASDIHLKVGLPPVVRRFGALIPLRDQSKLT
                     NEDIYAVAYGIMNQYQQAKFETTNEIDFAYSLSGIARFRVNCFKQRGSYGMVFRVVPT
                     DPPSLDDLNLPEVLKKIALERKGLILVTGTTGSGKSTTLAAMVNHINTNRNCHVITIE
                     DPIEFLHRDRKSIINQREVGSDTNSFAMALRAALRQDPDVILVGEMRDRETIDIALTA
                     AETGHLVLSTLHTLDAKETIMRIVSQYPPHQHLQIRLQLAGVLKAAISQRLLPRSDGQ
                     GVIPACEVLVSTALVRECIIDENRTSEISDAIADGTVTYGMQTFDQSLMALVKDGKVS
                     YHEALRNATNPNDFALRMKGIRGTSDSKWDQFEKEEQDSDAEGRTVIFQEGKYRDVE"
     gene            complement(5560..6582)
                     /gene="recA"
                     /locus_tag="PLT69_02105"
     CDS             complement(5560..6582)
                     /gene="recA"
                     /locus_tag="PLT69_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOJ13273.1"
                     /translation="MDEGRKRALEDAISKIERSFGKGSIMRMGSDAIGKGVPVISTGS
                     LLLDFEALAIGGIPRGRVTEIYGAESSGKTTLSLHCIASAQASGGVAAFIDAEHALDV
                     TYAQKLGVDIDNLLVSQPDSGEQALEIAETLVRSNAVDIVVIDSVAALVPKAEIEGDM
                     GDAHMGLQARLMSQALRKLSGAISKTNTTAVFINQTRQKIGVLYGNPETTTGGNALKF
                     YASVRLQIKNVAPIKDGDRVVGSRTRVKVVKNKLAPPFKEVEFDIMFGEGISRYGELL
                     DVASSADKNIVEKSGSWYTYNKQRLGQGREAAKLFLKEHPEVYGEIYEEVKRVLAERS
                     GTQLKE"
     gene            complement(6584..7135)
                     /gene="thpR"
                     /locus_tag="PLT69_02110"
     CDS             complement(6584..7135)
                     /gene="thpR"
                     /locus_tag="PLT69_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOJ13274.1"
                     /translation="MIRAFVALDLPYELKGTLAAAVERLTERNRGVRWVRPEGMHLTL
                     KFLGDIPETDVQPIAGDLDVAAGPCSPLRLRLSGFGAFPGPKKARVVWVGVSGDTEAL
                     GSLAKRVDEACASHGIPRERRPFAGHITLGRLRVPSVVDLRVEPVQGAFVAEEIVLYR
                     SELSPGGARYTPLHKSRLKGTGG"
     gene            complement(7098..>7372)
                     /locus_tag="PLT69_02115"
     CDS             complement(7098..>7372)
                     /locus_tag="PLT69_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOJ13275.1"
                     /translation="AREMLSGVLRLTGADVGVATTGIAGPEGGSPDKPVGTVWICAGT
                     RGFADAVRYEFPFNRAGNKMAAAKTALFMLRNLVHDQGVRGPRPAV"
BASE COUNT         1116 a         2511 c         2340 g         1405 t
ORIGIN      
        1 gtgaacacga tgaaggccgg ccgaagcttc tcgtaatgcc tggcgagatc cagggcggtc
       61 atggaggtga cctctttcca cccggtgatc gccgccctgc cgcccagggc gtctatgccg
      121 atcgcgacgc gccccgggaa cgcctccagg atggacgagg cggtcgacgg gtcgcgtacg
      181 cacacggtgc ccaggatcac gtacttcacc cccaggtcga aggcccgcct ggcatcttcg
      241 acggtcctga tgccgccccc cagttccacc tcgatgccga gggccctcgc tatcgcgccg
      301 atggcgtcca ggttggcccc cctgccttgg gcggccgcgt cgaggtccac cacgtggatg
      361 cggccggcgc cggcgtcctc gaacgacctc gcgacagcca cggggtcgtc cgagtagacg
      421 gtgacctcgt tgtagtcacc cctggcgagc ctgaccacct ttccttgata caggtccacg
      481 gccgggatga cgatcatgtc ccgccgcctc cttcccgcct tacccaccag ctcgagaaga
      541 gggtggagta cctgagcagg tagaccgtct cgtgcgtgga ttccaccatg aagtactcgt
      601 ccgggtggaa ggacgggtcc gtgtatgcct cgtaccagtg ggcccgcacg gccctcacct
      661 ccacgctccc tccctcgaag gtgaacctca ggggcctgcc gggcctccgg gccccgtcca
      721 tggtctccac ctgggccgga gagaacctca cagcgtctcc ttcgtggacg ggacgccgac
      781 cccggtgacg gccgatgcct ctttgagggc gcgcgcaaag gccttgaagc acgcctctgc
      841 gaggtggtgg ccgttcgcgt gcgcccccac ggtcacgtgc atggtgatgc ccgcgttgaa
      901 ggcgagcgcc ctgaagaact cgacgacgag gtccaggtct atgtcgccgc agcggccctt
      961 gaacgcctct gcgccgtgaa ccgcgaagaa gggcctgccg gagaggtcga tgcacacctc
     1021 cacgagggcc tcgtccatcg ggaccttcgc gaggccgaag cgctcgatcc ccctcttgtc
     1081 ccccagggcg tccttgatgg ccttcccgag cacgatgccg atgtcctcga cggtgtggtg
     1141 gaggtccacg tggacgtcac cggaggcctt gaccacgagc cccatggatg cgtgtacccc
     1201 cagcgactcg agcatgtggg cgagaaatcc cgaggggacg gatatgtccg tcctgccgcc
     1261 ttccaggcag accttgaccg tgatgtccgt ctcccgcgtc ttcctcgtga ccgtggcctc
     1321 cctcatggct tcacccccgc agactcgagg aagatctcta tgaccttccc ggtggaatcc
     1381 gcacccgccc ctccctgggc gaacagctcc ttgccgcctc cccggccgcc caccgcggcc
     1441 atggccttct tcatcacgcg ccccgcgtcc atgaccccct cgaccccctt ggtcgccatg
     1501 atcatgaagg tggccttgtc gccctcgtcg gtgtacagga gcacggcgcc cttcgaaatg
     1561 cgctccttga tcctgtcgcc gacctggcgc atctgggcgg tgtccgcctg ctgcaccttc
     1621 ttcaccacca ccttcagccc tcccgcgtca tactcgcgtt cgtcggccga cggccccgtc
     1681 cccgtggcga gcttcaccgt gagttcctcg atggtcgcct cctggtcctt gatctttgcc
     1741 aagagcccct tgatgcgttc ctcgatctcc tcgggggagc acctcaggct ctgggatgca
     1801 ccggcgacga gggatgacag ggacctcgcg tgttccaggc acctgaaccc ggccaccgcc
     1861 tcgatcctgc ggaggccggc ggagatgctc gagacggaca cgaccttcac catgccgatc
     1921 tggcccgtgg acgagcagtg cgtccccccg cagagctccc tcgagacgtc ccccatgctc
     1981 acgagcctca ccgtgtcgcc gtatttctcc gtgaacaggg ccatggcgcc cctggagacc
     2041 gcctcgtccc tggagaggat ctcggtggtg atcggcagat cctccttgac gtactggttc
     2101 acgaggttct ccacgtgggc gagttcctcg gggctcaagg ccgagaagtg cgtgaagtcg
     2161 aacctgagcc tcgagtcgtc cacgagggac cccgcctggt gcacgtgttc cccgagcacc
     2221 tcgcggaggg ccctgtggag caggtgcgtc gcgctgtggt ggcgcatgac cgccttcctc
     2281 ctgtgcgcgt ccaccctgag cgtcaccccc tggccgagct cgagcctgcc cgagacgacg
     2341 cggaccctgt ggaccaccgt gtccgactcg gtcttcacgg cgtctaagac ccgggccctg
     2401 cccgagggcg agacgatctc gcccgtgtcg ccgacctgcc cgccggactc gccgtagaac
     2461 ggcgtcacgg ccgtcgccac cagaagctcc tcgccctctt cggcggcctc gagctcggac
     2521 gaggcgtcca tggaacgcat ctcgacgatg agcgagtccg tctcgagggt gtcgtatccc
     2581 acgaatctcg agccggatgc cgccgaccac gcgctctcga gccccgtgcc ggcgaaggag
     2641 ctcgcccccc tcgccttttc ccgctggatc tccatctccg cctcgaatcc cgcggtgtcg
     2701 atctcgatgc cctccttgcg ggcgatgtcc gcggtcaaat ccacggggaa gccgaaggtg
     2761 tcgtagagga caaagacctc cctgccgggc atgagcgtct tgccgttcgc cttgagatcc
     2821 cgcacgatct cgtcgaggag gccgaggccc ctgtcgaggg tcttgaggaa acgttcctcc
     2881 tcggaggcga tcaccttgtc cacgaactcg ctcctcatgg cgagctcagg gtacgcgggg
     2941 gacatgtccg atgccacccg catcgccacc ctgtggagga atggcctgtc gaatcccagc
     3001 atcttgccgt gcctggcggc cctgcggatg atcctcctga gcacgtagcc gcggccctcg
     3061 ttggagggca gcacaccgtc cgagacgagg aaggcgcacg cacgggcgtg gtcggcgacc
     3121 acgcggatgg acacgtccgt ttccccgtcg ttcccgtagg agacccccga gagctcgccg
     3181 atgaagccga tcaggcccgt gaacaggtcg gtgtggtagt tgctcgtcac cccctggagc
     3241 accgccgtga tgcgttcgag ccccatgccg gtgtctatgt tcggggccgg cagccgagtc
     3301 aggttcccct gctcgtcttt gttgaactgc atgaacacga ggttccagat ctccaggtaa
     3361 cggtcgcagt cgcactcgcc cggggagcac tcgggcctgg cgcaccccac gcccggaccc
     3421 tggtcgaaga tgatctcgga gcaggggccg caggggccgg tgtcgcccat ggcccagaag
     3481 ttgtccttgt cccccaggcg gaggatcctg tcggacgcga cgccgatggt gcggttccag
     3541 atatcgtagg cctcgtcgtc ggtgtggtgg acgctcacgt agagcctcga ggggtcgagc
     3601 cccatccggt cgaccaggag ctcccacgcg tactcgatgg caccgcgctt gaagtagtcg
     3661 ccgaaggaga agttgccgag catctcgaag aaggtgtggt gcctggccgt ccgtcccacg
     3721 ttctcgaggt cgttgtgctt gccgcccgcc ctgacgcact tctgcgatga cgtggcgcgc
     3781 gagtacggcc ttttctgtgt gcccaggaag gtcgccttga actgcaccat gcccgcgttg
     3841 gtgaagagca acgtggggtc gtccgacggc accagggacg agctgggaac gagggtgtgg
     3901 cccttgtcct ggaagtagtc gaggaacatg gtgcgaatgt ctgaacctct catctgtcaa
     3961 ctccgttcgt catgtcgatc accaggtcca acgggaatcc cctgcccatg aggaacctga
     4021 tgagtctcgt cctgtcggtc ccagacctct tctcgaggag cctcgccgcc ctgctgcgct
     4081 cgttcagctc gggaggcagt tctcgaacgg cccggcgggc gtcctgctcc gggaacccct
     4141 cgtgttcgag cgaggccaag atggcgtagt caccgtatcc cctttccgcg aggacgacgg
     4201 ccctgcgcct cgcgtactcc ccgtcgtcga ggtagccgag ctccgtcagc ctgtcgacgc
     4261 acgaggcgat cacgtcctca ccggcgccct tgcgctcgag ttttttttta gctcgtggct
     4321 cgagacgctc cgcctggaga ggatgccgag cgcgtacgcc agggcgtcac tcgacgtcgc
     4381 gatacttgcc ttcctggaag atgaccgtgc gtccctcggc atcggagtct tgctcctcct
     4441 tctcgaactg gtcccacttg ctgtcggacg tgccgcggat gcccttcatg cgcagggcga
     4501 agtcgttcgg gttggtcgcg ttcctcaggg cctcgtggta gctgaccttc ccgtccttga
     4561 cgagcgccat gagagactgg tcgaaggtct gcatgccgta ggtgacggtg ccgtcggcta
     4621 tggcgtcgga gatctcggac gtccggttct cgtcgatgat gcattcgcgc acgagcgccg
     4681 tcgagacgag cacctcgcag gcgggtatga cgccctgccc gtcgcttctg ggcagcaggc
     4741 gctgcgagat ggccgccttc aggacccccg ccagctggag cctgatctgg aggtgctggt
     4801 gcggcgggta ctgcgacacg atgcgcatga tggtttcctt ggcgtccaag gtgtgcaggg
     4861 tcgacagaac gaggtggccc gtctccgcag cggtcagcgc gatgtcgatg gtctcgcggt
     4921 cgcgcatctc cccgacgagg atgacgtcgg ggtcctgcct gagggccgcc ctcagggcca
     4981 tcgcgaagga attcgtgtcc gagcccacct cgcgctggtt gatgatggac ttgcggtccc
     5041 tgtggaggaa ctctatgggg tcctcgatgg tgatcacgtg gcagttccgg ttcgtgttga
     5101 tgtggttcac catggccgcg agcgtggtcg acttgccgga accggtggtg cccgtcacca
     5161 ggatgagccc ctttcgctcc agggcgatct tcttgaggac ctcggggagg ttgagatcgt
     5221 ccaggctggg cgggtccgtg ggtacgaccc tgaagaccat gccgtagctg cccctctgct
     5281 tgaagcagtt gaccctgaac ctcgcgatcc cgctcaggct gtaggcgaag tcgatctcgt
     5341 tggtggtctc gaacttcgcc tgctggtact ggttcatgat gccgtaggcg accgcgtaga
     5401 tgtcctcgtt ggtgagcttg ctctggtctc taagcgggat gagggcgccg aagcggcgca
     5461 ccacgggcgg cagccctacc ttgagatgga tgtcggacgc cgttatctcg acggcatgcc
     5521 tcaggatcgg attgatgtcc atgcgcaccc ccgttcaggt cactccttga gctgggtccc
     5581 cgaacgctcg gccaagaccc tcttgacctc ttcgtagatc tccccgtaga cctccgggtg
     5641 ctccttgagg aagagcttcg ccgcctcgcg tccctggccc aaccgctgtt tgttgtacgt
     5701 gtaccacgag cccgattttt ccacgatgtt cttatcggcc gacgatgcca cgtccaggag
     5761 ttcgccgtaa cgggagatgc cctcgccgaa catgatgtcg aactcgacct ccttgaaagg
     5821 cggtgcgagc ttgttcttca cgaccttgac acgggtcctg ctccccacga ccctgtcgcc
     5881 gtccttgatc ggcgccacgt tctttatctg caggcgcacg ctcgcgtaga acttgagcgc
     5941 gttgccgccc gtcgtcgtct cggggttgcc gtagagcacg cctatcttct gtcgagtctg
     6001 gttgatgaac acggccgtgg tgttggtctt gctgatggcg ccggagagct tgcgcagggc
     6061 ctgcgacatg aggcgcgcct gcagacccat gtgcgcgtcg cccatgtcgc cctcgatctc
     6121 ggccttgggc acgagcgcgg cgacggagtc tatgacgacg atgtccaccg cgttggagcg
     6181 cacgagggtc tccgcgatct cgagggcctg ctctcccgag tcgggctggg agacgaggag
     6241 gttgtcgatg tccacgccga gcttctgggc gtaggtgacg tcgagggcgt gctcggcgtc
     6301 gatgaacgcg gcgacgcccc ccgaggcctg ggccgaggct atgcagtgga gcgacagggt
     6361 ggtcttcccg gaggactccg cgccgtagat ctcggtcacc ctgcccctgg ggatgccgcc
     6421 tatggccagc gcctcgaagt ccagcaggag ggacccggtg gagatgaccg gcacgccctt
     6481 gcctatggcg tccgatccca tgcgcatgat ggagcccttg ccgaaggacc tctctatctt
     6541 gcttatcgcg tcttccagcg cccgcttcct tccctcgtcc atgtcaacct cccgtgcctt
     6601 tcagcctgct tttgtgcagc ggcgtgtagc gcgcgccgcc cggggacagc tcgctgcgat
     6661 acaggacgat ctcctcggcc acgaacgcgc cctgcaccgg ttcgaccctg agatctacca
     6721 cagaaggaac cctgagtcta ccgagggtga tgtggcctgc aaacggcctt cgctccctgg
     6781 ggattccgtg agaggcgcag gcctcgtcca cccttttcgc gagcgacccc agggcctcgg
     6841 tgtcaccgct gacgcccacc cagaccaccc gggccttctt ggggccggga aacgcgccga
     6901 accccgacag gcggagcctc agcggggaac aagggccggc ggcgacgtcc aggtcgccgg
     6961 cgatgggctg cacatcggtt tcagggatgt cgcccaggaa cttgagcgtg agatgcatgc
     7021 cctcgggtct cacccacctc acgccccggt tgcgttccgt gaggcgctcg acggccgccg
     7081 cgagagtgcc tttcagttca tacggcaggt ctagggccac gaacgccctg atcatggaca
     7141 aggttcctca gcatgaagag ggcggtcttg gcagcggcca tcttgttgcc cgcccggttg
     7201 aaggggaatt cgtaccgcac ggcgtcggca aagcccctgg tgccggcgca gatccacacc
     7261 gtgcccacgg gcttgtccgg tgacccgccc tcggggccgg cgatgccggt ggtggcgacg
     7321 ccgacgtccg cgcccgtgag cctgagaacg cccgaaagca tttcccgtgc cg
//
LOCUS       DAOSCR010000045         7346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6500_length_7346_cov_3.339322, whole genome shotgun sequence.
ACCESSION   DAOSCR010000045 DAOSCR010000000
VERSION     DAOSCR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6500_length_7346_cov_3.339322"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLT69_02120"
     CDS             complement(<1..290)
                     /locus_tag="PLT69_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOJ13276.1"
                     /translation="MHMADALLSPAVGGTMWAVTGAKVAHCAKKMKSLGSERLVPLMG
                     VLGAFVFAAQMINFSIPATGSSGHLGGGLLLAAILGPHAAFIVIASVLVVPAL"
     gene            complement(546..962)
                     /gene="nikR"
                     /locus_tag="PLT69_02125"
     CDS             complement(546..962)
                     /gene="nikR"
                     /locus_tag="PLT69_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOJ13277.1"
                     /translation="MSRLVRFGVSLESGLLEQFDRLIAKRGYTSRSEALRDLIRQELV
                     HEEWEEDREVAGTVTFLYDHHTRGLSARLTDIQHDHQDLIISTQHIHLDHHTCLEIVA
                     VRGRASDVLKLANALRSVKGVRHGSLSMSSTGKTLL"
     gene            complement(972..1205)
                     /locus_tag="PLT69_02130"
     CDS             complement(972..1205)
                     /locus_tag="PLT69_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOJ13278.1"
                     /translation="MTNRGRKPLRMKQGELSAELVKRNYYIDERYAKALSVLSALTGK
                     ELSILVNEALQDLIAKYQPSTEIDLLNIKPIKK"
     gene            complement(1245..1319)
                     /locus_tag="PLT69_02135"
     tRNA            complement(1245..1319)
                     /locus_tag="PLT69_02135"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1285..1287),aa:Cys,seq:gca)
     gene            complement(1896..2873)
                     /locus_tag="PLT69_02140"
     CDS             complement(1896..2873)
                     /locus_tag="PLT69_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ13279.1"
                     /translation="MGRSFREVVLARLARAAVFFVRKAPYPLVTAVFKAVALCAWCFL
                     PYHRKTSAVQLRMALGLENPTLMSLRVFLGHAEILVDAVRYAYMSGEEVKRAVRVEGL
                     EHLERALETGKGVMMITGHVGNWEILSHLPRILSVEFCVMAQRRGDPMIDAIVDDLRS
                     RSGATILPPTGKALMLVRELRKGRVIGMVVDGRHDGADAVMCEVFGMPAPTNPAPAFI
                     AIKGDAVVLPVVAIKNGRCYRVIFTRPVPASDFGASRDAVERLSGWMQSQVEHAVRRD
                     PHQWFWLYSRWIPRSLMKKVVREGLDLEGVVSGAALRRGWTPPGGAPLR"
     gene            complement(2888..3847)
                     /locus_tag="PLT69_02145"
     CDS             complement(2888..3847)
                     /locus_tag="PLT69_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ13280.1"
                     /translation="MNVLKEAPSVLQALLVRGAIRLVLALPYRWALSIGRVLGLFTWA
                     VLPTRRRLVEVQARSAIGEEATRSFVLKVFMNQGEILVDTIRYAFMDEDELKSRIRIE
                     GRENLEEALEQGRGVMMITGHIGNWEILSNLPRLVGIQFCVMADRREDERLEAVIDGI
                     RRRSGATILPPKGKALMLVKELRKGRLIGMVVDQRGRRRDNLFCDVFGMPAPTNPAPA
                     FIAIKGDALVLPVYAMKEEGGYVIRFDPLVDTRRFSPGADGIEALSRFMQTWVSTTVR
                     KNPTWWFWLHSRWIKRKTFRSLARSGGDFRALVRSMNRPVTRE"
     gene            complement(3844..4446)
                     /locus_tag="PLT69_02150"
     CDS             complement(3844..4446)
                     /locus_tag="PLT69_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ13281.1"
                     /translation="MRSSAWSRRAVFMDRDDTLIRDTGYLSDPAAVEPLEGACEAVRR
                     LNEAGIPVIVVTNQSGVARGFFDEERLQEIHSRLVEVFADMGARIDAVYSCPHHPEGV
                     VERYRLACRCRKPEPGMLIDAARDFGLDLGSCFLVGDKPHDIEAIHRVGGRGVLVGDP
                     VGVDAPNPALMPDFVARDIRQAVEWILGKIMAEGGEGGGG"
     gene            complement(4397..5380)
                     /gene="lpxK"
                     /locus_tag="PLT69_02155"
     CDS             complement(4397..5380)
                     /gene="lpxK"
                     /locus_tag="PLT69_02155"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOJ13282.1"
                     /translation="MRSSSAALRALSTVYGALAAARNRLYDTGVLRPTRVPGVVVSVG
                     NIEAGGTGKTPFVMALAGGLAARGVNVAVVTRGYKGRIRGPVHVRPGHTSLEVGDEAL
                     LMALSLARVPVVKSPDRVRGALFARARFGAEAILLDDAFQHRRIHRDLDIVLVARDLP
                     KERLLPAGMLREPAGSLARAHHVIAMKGCPLPYPKADLAPVCLVDTRWRTAPLELVRD
                     EDVLAFCAVGSPGHFFETAGTLCRVVDTIAFADHHRYTAADARAIAERAARAGYVLTT
                     EKDLVRMEPGMFGELEGRLFALRVALSMEGLEEIEDEIQRMVETRRVHGQG"
     gene            complement(5355..7091)
                     /gene="msbA"
                     /locus_tag="PLT69_02160"
     CDS             complement(5355..7091)
                     /gene="msbA"
                     /locus_tag="PLT69_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOJ13283.1"
                     /translation="MRDFGTYMRLLGYVKPHRFKLLGSLALTTVVAGAMPAIALLAKY
                     VMDDVFIARDVVMLKLVAAAVVAIYLVKSVADYLSYYFMNDVGQSVIRDIRDRLYAHI
                     QTLSMPFFTRTPTGVLISRITNDVNLVQLSVTESVTEFIRQTVTLTGLIAAVFYRDWQ
                     LALISMVVFPLVIYPINKFGQRLKRYSTKGMKVMGNVMSILDEAISGIRIVKAYTMEE
                     YEYRRFSDENHDFYRNWMKRTRVRALSGPMMELIGGLATAGLLVYGGLRVIDGVMTPG
                     DFMSFITGLSLLYSPIRKLNKVYIEIQEGTAAARRIFDLLDTPAEIRDDPDAMELGRA
                     RGDFEFKNVWFSYSGDDTYALRDVSFRVDSGQTVAIVGESGSGKSTIANLLPRFYDVT
                     KGAVIVSGTDIRKVTMRSLRENIALVTQDAVLFNDTIKANIAYGSKDATMEAIVAAAR
                     AANAHDFIEAMPQGYDTVIGESGVRLSGGQRQRLCIARAILKDAPILVLDEATSSLDA
                     ESEREVQAALEVLMEGRTSLVIAHRLSTVINAHRIIVLHQGRIVEEGTHEELLARQGH
                     YARLYALQLGSP"
     gene            complement(7088..>7346)
                     /locus_tag="PLT69_02165"
     CDS             complement(7088..>7346)
                     /locus_tag="PLT69_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOJ13284.1"
                     /translation="RLARAFVRIDYIGMPNIIAGRSIVPELVQGGLTAEALAEAVTGF
                     VTNRSLYEATREALLEIRGRLGSLRASREVARWAVQLMEGA"
BASE COUNT         1213 a         2465 c         2358 g         1310 t
ORIGIN      
        1 agggccggta ccacgaggac cgatgcgatg acgatgaacg ccgcgtgtgg gccgaggatc
       61 gcggcgagca acaggccccc tcccaggtgg ccgctggaac cggtggccgg gatgctgaag
      121 ttgatcatct gtgcggcgaa gacgaaggcc ccgagcacgc ccatgagggg taccaggcgc
      181 tccgacccca ggctcttcat cttctttgcg cagtgggcca ccttggcgcc cgtgacggcc
      241 cacatggtcc ctccgacggc aggggaaagc agtgcgtcag ccatgtgcat gcggcaaaac
      301 ctcctcgact ggtttcatga tctgtcagta gcatgattgg gaaaaaagtg ctaccccgat
      361 gtcataccgg gtgccgtgcg ggtcatgacc tgccgtcaga tgagggagag ggcctgtcag
      421 ggcgatgctc cccgacgcca cgccgagcct ggggcagctg tgagaaaacc cgtgaccttg
      481 cagggggagg gttttcctcg acgccctggc agggcaaact tccgtccccg gttggggtgg
      541 gggcctcaaa ggagggtctt gcccgtgctg ctcatgctca gggagccgtg acgcaccccc
      601 ttgaccgatc tcagggcgtt ggccagcttc agcacgtcgg acgcacggcc cctcaccgcc
      661 acgatctcga ggcaggtgtg gtggtccagg tggatgtgct gggtcgagat gatgaggtcc
      721 tggtggtcgt gctggatgtc cgtgagcctg gcggagaggc cccgggtgtg gtggtcgtag
      781 agaaaggtga cggtgccggc gacctccctg tcctcctccc actcctcgtg gacgagctcc
      841 tgccggatga ggtcgcgcaa ggcctcggac ctggacgtgt agcccctctt ggcgatcagg
      901 cggtcgaact gttcgaggag cccgctttcc agcgagacac cgaacctcac gaggcgggac
      961 atgggatgac cttacttctt gatgggcttg atgttcaaga ggtcgatctc ggtggacggc
     1021 tggtacttgg ctatcaggtc ctgcaaggcc tcgttcacca ggatcgaaag ttccttgccg
     1081 gtcagcgccg agaggacgga gagggccttg gcgtaacgct cgtcgatgta gtagttccgc
     1141 ttgacgagct ctgcagaaag ctcgccctgt ttcatcctca gcggttttct gcccctgttg
     1201 gtcatgctcg tcctcccggg gaagtgtcta catggggttg tcggtggagg cggcaaccgg
     1261 attcgaaccg gtgaataacg gttttgcaga ccgtcgcctt accccttggc tatgccgccg
     1321 gacctttgcg tctccttgat ataccggcgg gtgtctcgaa aagtcaaggg caagctaaac
     1381 tccggcttcc ggcctcggca agcggccggg cagaggcgtg ctggcatccc cccgcgtgtt
     1441 aacccccacc cccttgggga gggcgtccac cagccgcatg ccgtcaacgt gtcgggcgtc
     1501 acacctgcac tcccttgggg agggcgcctc gggtgcagcc cctggaaggg gcccgtttcc
     1561 ccacccttgg gcatcgcctg catgtccgcc acgccggggc ctgccttccc cacgagaaca
     1621 caagccctgc ccgggcagaa ggcctccgcc cggacacgca cagctcgagc atgcagggag
     1681 cggcctcagg agagcatgcg ggtgaccaac aacgcgctcc actaccggct cacagcccga
     1741 gcatgcaggg aaagaggcct ccggagcacg ccgacccccg gcaggtaagg cgcgccaact
     1801 gatgtgccgc acgcagccgt cacatgaccc cagagggctt cgggggcttg cggtccttct
     1861 cccggaacgc agcagataga gacagcgtgg acggatcacc gaaggggcgc gccgcccggc
     1921 ggtgtccagc ccctgcgaag agccgcgcct gacacgacac cctcgaggtc gagcccctcc
     1981 cggaccacct tcttcatgag agagcggggg atccacctgc tgtagagcca gaaccactgg
     2041 tggggatccc tcctcaccgc gtgttccacc tgggactgca tccaaccgct gagcctctcc
     2101 acggcatccc tcgaggcgcc gaaatccgag gccggaacgg gccttgtgaa gatcacgcgg
     2161 tagcaccggc cgttcttgat cgccaccacc ggcaggacca cggcgtcacc cttgatcgcg
     2221 atgaaggcgg gtgccgggtt cgtgggtgca ggcatgccga agacctcgca catgaccgcg
     2281 tccgcgccgt cgtgcctgcc gtccacgacc atgccgatga cacggccttt cctgagctcg
     2341 cgcacgagca tgagcgcctt gcccgtaggc ggcaggatgg tggcaccgct ccgcgagcga
     2401 aggtcgtcca cgatggcgtc gatcatgggg tctccccttc gctgggccat gacgcagaac
     2461 tcgaccgaga ggatgcgggg gaggtgcgag aggatctccc agttgcccac gtggcccgtg
     2521 atcatcatga cccccttgcc ggtttcgagg gcgcgctcga ggtgctcgag cccctcgacc
     2581 ctgaccgccc ttttcacctc ctccccgctc atgtacgcgt acctgacggc gtccacgagg
     2641 atctctgcat ggccgaggaa gaccctgagc gacatgagcg tggggttttc cagccccagg
     2701 gccatgcgga gctgaacggc cgatgtcttc ctgtggtagg gcaggaaaca ccacgcacac
     2761 agggcgaccg ccttgaagac ggcggtcacg agggggtagg gggccttcct cacgaagaag
     2821 acggccgcac gtgccagccg ggccaggacg acctctctga acgaccttcc catgcccacc
     2881 gctggggtca ctccctcgtg acgggcctgt tcatcgagcg cacgagcgcc ctgaagtcgc
     2941 cgcccgacct cgccagggac cgaaacgtct ttcgcttgat ccacctgctg tggagccaga
     3001 accaccatgt ggggttcttc ctcaccgtgg tgctcaccca tgtctgcatg aaccggctga
     3061 gggcctcgat gccgtcggcg ccggggctga accggcgcgt gtcgacgaga ggatcgaacc
     3121 tgatcacgta gcctccttcc tccttcatgg catagacagg cagcacgagg gcgtccccct
     3181 tgatcgctat gaaggcgggc gcagggttcg tgggggcggg catgccgaac acgtcgcaga
     3241 acaggttgtc tctccgcctt ccccgctggt ccaccaccat cccgatcagc cggcccttcc
     3301 tgagctcctt gacgagcatg agcgcctttc ccttgggagg caggatcgtg gccccgctcc
     3361 ttctcctgat gccgtctatg acggcctcga gcctctcgtc ctccctcctg tccgccatga
     3421 cgcagaactg tatccccaca agcctcggca ggttcgagag gatctcccag ttgcctatgt
     3481 ggcccgtgat catcatgaca ccccggccct gctcgagcgc ctcttcgagg ttctcccgcc
     3541 cctcgatcct tatgcgcgac ttcagctcgt cctcgtccat gaaggcgtag cggatggtgt
     3601 ccacgaggat ttccccctgg ttcatgaaca ccttgaggac gaaggagcgc gtggcctcct
     3661 ccccgatggc actcctggcc tgtacctcga cgagcctgcg ccttgtgggc aggacggccc
     3721 acgtgaagag gccgagcacc ctgcctatgg acaaggccca ccggtagggc agggccagga
     3781 cgagcctgat cgccccccgc acgagcaggg cctgcaggac gctcggcgcc tccttgagga
     3841 cgttcatccg ccccctcctt ccccgccttc cgccatgatc ttcccgagta tccattccac
     3901 tgcttgacgt atgtctctcg ccacgaagtc gggcatcaat gcagggttcg gggcatcgac
     3961 gccgaccggg tctcctacca ggacccctct ccccccgacc ctgtgtatcg cctcgatgtc
     4021 gtgaggtttg tctcctacca ggaagcacga gccgaggtcc aggccgaaat cccgtgctgc
     4081 atcgatgagc atgcccggtt cgggcttccg gcacctgcag gcgagccggt agcgctcgac
     4141 cacgccctcg gggtggtggg gacaggagta gacggcgtcg atgcgcgcgc ccatgtcggc
     4201 gaagacctcg acgagcctcg agtggatctc ctgcaagcgt tcctcgtcga agaaacccct
     4261 ggcgacaccc gactggttgg tcaccacgat caccggtatg cccgcctcgt tgagcctccg
     4321 cacggcctcg cacgcgccct cgaggggctc gaccgccgcg gggtcgctga ggtagcccgt
     4381 gtccctgatc agggtgtcat ccctgtccat gaacacggcg cgtctcgacc atgcgctgga
     4441 tctcatcctc gatctcctcg agcccttcca tggacagggc gaccctgagg gcgaagagcc
     4501 tcccctcgag ctccccgaac atgccgggct ccatcctcac gaggtctttc tcggtggtga
     4561 gcacgtaccc ggccctggcc gccctctctg cgatggcacg ggcgtctgcg gcggtgtagc
     4621 ggtggtggtc cgcgaaggcg atggtgtcga ccacacggca cagcgtgccc gcggtctcga
     4681 agaagtggcc cggggagccc acggcgcaga aggcgaggac gtcctcgtcc cggacgagct
     4741 cgaggggggc tgtcctccac cgcgtgtcca ccaggcagac gggcgccagg tccgccttcg
     4801 ggtacggcaa ggggcagccc ttcatggcta tgacgtggtg cgcccgcgcc agggatcctg
     4861 ccggctccct gagcatgccc gcgggaagga gtctctcttt cgggaggtcc ctcgcgacga
     4921 gcacgatgtc gagatccctg tggatcctcc tgtgctggaa ggcgtcgtcc aggagtatcg
     4981 cctcggcccc gaagcgcgcc ctcgcaaaga gcgcccccct gacgcggtcc gggctcttga
     5041 cgaccggcac ccgagcgagg ctcagcgcca tgagcagggc ctcgtcaccc acctcgaggc
     5101 tcgtgtggcc cggtctcacg tgcacgggac cgcggatcct ccccttgtag cccctggtga
     5161 cgacggcgac gttcactccc ctggcagcca gcccgcctgc gagtgccatg acgaaggggg
     5221 tcttgccggt cccgccggcc tcgatgttgc ccacgctcac caccaccccc gggacccgtg
     5281 tgggcctgag caccccggtg tcgtagaggc ggttccgggc ggccgcgagc gcgccgtaga
     5341 cggtggagag agccctaagg gctgccgagc tggagcgcat agagcctcgc gtagtgaccc
     5401 tgcctggcca ggagttcctc gtgggtccct tcttccacga tcctcccctg gtggagcacg
     5461 atgatgcggt gggcgttgat caccgtggag agcctgtggg cgatcaccag ggacgtcctg
     5521 ccctccatga gcacctcgag ggccgcctgg acctcgcgtt cgctctccgc gtccagcgag
     5581 gaggtcgcct cgtcgaggac gaggatcggg gcgtccttga ggatggcccg tgctatgcag
     5641 agccgctggc gctgcccccc cgagagcctc accccgctct cgccgatcac ggtgtcgtac
     5701 ccttgtggca tggcctcgat gaagtcgtgg gcgttggccg ccctggccgc cgccacgatc
     5761 gcctccatgg tcgcgtcctt cgaaccgtag gcgatgttcg cctttatcgt gtcgttgaac
     5821 aggaccgcat cctgggtcac gagcgcgatg ttttccctca aagaccgcat ggtgaccttg
     5881 cggatgtcgg tgcccgagac gatgacggcg cccttggtca cgtcgtagaa acggggaagg
     5941 aggttcgcga tcgtcgactt cccggacccg gactcgccca cgatggccac cgtctggccg
     6001 ctgtcgaccc tgaagctcac gtccctgagg gcgtaggtgt cgtcgccgga gtaactgaac
     6061 cagacgttct tgaactcgaa gtcacccctg gcgcgtccga gctccatcgc atcagggtcg
     6121 tcccgtatct cggcgggggt gtcgaggagg tcgaagatgc gccgtgcggc cgctgtccct
     6181 tcctggatct cgatgtacac cttgttgagc ttgcgtatgg gagagtacag gaggctcagg
     6241 cccgtgatga aggacatgaa atcgcccggc gtcatgacgc cgtcgatcac ccgcaggccc
     6301 ccgtagacga ggagcccggc cgtggccagc ccgccgatga gctccatcat gggcccggaa
     6361 agggccctca ccctcgtgcg cttcatccag ttccggtaga agtcgtggtt ctcgtcggag
     6421 aagcgccggt actcgtactc ctccatggtg taggccttca cgatcctgat gccgctgatg
     6481 gcctcgtcga ggatgctcat gacgttgccc atgaccttca tccccttggt cgagtaccgc
     6541 ttgagcctct ggccgaactt gttgatgggg taaatcacca gcgggaacac caccatggag
     6601 atgagggcga gctgccagtc ccggtagaag accgcggcga tgagccccgt gagcgtgacc
     6661 gtctgcctga tgaactcggt gaccgattcg gtgaccgaga gctgcacgag gttgacgtcg
     6721 ttggtgatcc ggctgatgag cacgcccgtg ggcgtgcgcg tgaagaaggg catggagagc
     6781 gtctggatgt gggcgtacag ccggtcgcgt atgtccctga tgacgctctg tcccacgtcg
     6841 ttcatgaagt aatagctgag gtagtcggcg accgacttca ccaggtagat ggcaaccacc
     6901 gcggccgcca cgagcttgag catgaccacg tccctggcga tgaagacgtc gtccatgacg
     6961 tatttcgcaa ggagcgctat cgccggcatg gccccggcga ccacggtcgt cagcgcgaga
     7021 gacccgagga gcttgaacct gtggggcttc acgtagccca aaagcctcat gtaggtcccg
     7081 aagtccctca tgccccctcc atgagctgca cggcccagcg cgccacctcc cgggatgcgc
     7141 gcaaggagcc gagacgtccc ctgatctcga gcagggcctc gcgcgtggcc tcgtagaggg
     7201 accggttcgt gacgaagccc gtcaccgcct cggcgagggc ctccgcggtc agcccaccct
     7261 ggacgagctc cgggacgatg ctcctgcctg ctatgatgtt gggcatgccg atgtaatcga
     7321 tccgcacgaa ggcgcgggcc agccgg
//
LOCUS       DAOSCR010000046         7339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6509_length_7339_cov_3.226387, whole genome shotgun sequence.
ACCESSION   DAOSCR010000046 DAOSCR010000000
VERSION     DAOSCR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6509_length_7339_cov_3.226387"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLT69_02170"
     CDS             <1..816
                     /locus_tag="PLT69_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ13285.1"
                     /translation="IGCAFIVSLFLCPGAWAERTVTVALGGDVFHMKEKSAKGGFEGY
                     EIDLMKAVAQEAGFKIKFVEVPWKSLFDGLNEGKYDAVIASVNITSGRKERYELSDPY
                     FSAAQLLVVPRSKVNMKLSGRNVGVFRLSAAADRIRRSGANITYYTVQETPQAFKDLQ
                     KGTIDALLCDSPVAIGCTAGSTSSSLCIASLDILDAKECVEEYGIVCRKGNRELVELI
                     NKGLASVKAKKLDNEFALRWFKGTAVASTSQGSGSFFASEIQTGPQNSPVSSK"
     gene            complement(891..1223)
                     /locus_tag="PLT69_02175"
     CDS             complement(891..1223)
                     /locus_tag="PLT69_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOJ13286.1"
                     /translation="MKGRLLREEKTIRVMIEMYCRDRHGSKGALCSRCRELCEYAEKR
                     LRNCPYGAKKPTCAACPIHCYQGGMREEVRRVMRYAGPRMMFEHPLLAIGHFIDGLAC
                     VRPPRPGA"
     gene            1746..3425
                     /locus_tag="PLT69_02180"
     CDS             1746..3425
                     /locus_tag="PLT69_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ13287.1"
                     /translation="MKIIYLTDIHGDFERMKSVLSETVANVYIIGGDLIDIPFYSMET
                     SIRYHELQSFFHGLRMKMGQEDRLLEDFVDQLLEQPDVPEDVQDKGTMYQQYTIRARR
                     VMQQKYKVLKNLVSMKKASTVLFLPGNYDMDLKYTALHDHDLHLHWYAIGDVRICGYG
                     GAEVRTPGIPERYIVRYLEGLSPRGRKNGKKNEMYSFFRVVKPHIIVAHQPAYGINDN
                     TTAGGAYGSPSLRTFCDENPVLLCLTGHAHDSWGVKYSGKTLYLNPSNFGEVTGPTGE
                     VNEGGFFYQIEMDQKEVKSIVFRKFHDGRIYDVADYTLENGTLVEKIIDRERYAALVD
                     RRNYDMKGVRYSHIPEIRLYKEIKDFFRMYQTPEAEERVDRIEEVARVLEEKTGREVA
                     MDVMGSVNMGISEPDSDIDLVLYVRCSSTCVDDVNVCETSARMQAEVKGLLGESIKAH
                     VMDCIDLDQVERSIRERDYECEVLQRFVAYRSICRPINYRVIAPIEDLLNSDISFRKE
                     VEGSTQSYIRIFANTSAHVRSIKKYEARLNDLGIRVPESVKRAIKQYLRED"
     gene            3758..4519
                     /locus_tag="PLT69_02185"
     CDS             3758..4519
                     /locus_tag="PLT69_02185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ13288.1"
                     /translation="MKNILYNLFIREVSLLCYRTCLDYVPGGSRILDVGIGNGAMIQE
                     FHETIRSKGISITGIDVDRCYLDHCERQIRKNSLEEYVNVFHVPVERFEPEEPHSFDF
                     VLFSMSFMLLDDPALVLRRIRRWVRPSGGVMFFQTMFARRSLLLDIVKPRLRYLTTVE
                     FGRVVYDDEFFGLLRREGVSVVEDVTVAKAWFGGRYRFVVCRPETAADVFTLPKAEIV
                     GPGDALDAPYRTSRASRTLRRNAFRSRSSGKDVER"
     gene            4516..5007
                     /locus_tag="PLT69_02190"
     CDS             4516..5007
                     /locus_tag="PLT69_02190"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOJ13289.1"
                     /translation="MTNPGRLVHTHVVAPLKALLNQGITPGRLAASMACGITVGVSPL
                     VGTTTAFCTMLALALRMNLLAIQLGNWLALPVQLALIAPFLMMGNRLFGDGRHHDWAS
                     LALLVREDAMLAVQAASRMVIHGALAWALCAPAVFAALFALLLPAFRMLGGLNQRSTG
                     KKG"
     gene            5081..5662
                     /locus_tag="PLT69_02195"
     CDS             5081..5662
                     /locus_tag="PLT69_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOJ13290.1"
                     /translation="MDKGREPCRRSRILLHVCCAPCATWPVERLSREGYEAVLFFSNS
                     NVHPEEEYTRRLDEARRLAGILGLELVEDVYDHEAWLDAVRGLEAEPERGKRCMKCFA
                     YNLGRAALAMGRLGLSRFTTTLTVSRHKSSRDVFAAARTMPGFLAVDFKKQDGYHQSV
                     VLSRGLKLYRQGYCGCEFSRQAALRDSPASGKT"
     gene            complement(5801..6916)
                     /locus_tag="PLT69_02200"
     CDS             complement(5801..6916)
                     /locus_tag="PLT69_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13291.1"
                     /translation="MSGPILEARDLKVSRGGTPVLDVREFTLNPGEFLSVIGPNGSGK
                     STLLLSLAGLLELGSGELRFGGVKIAGGRGMLEYRRRIAMVFQEPLLFDSTVYANVAS
                     GLAIRGMGRHETRDRVRENLELFGIAHLSDRSARKLSGGEAQRTSLARAFATQPEIVF
                     MDEPFSSLDPPTKESIVEDLSRVIRLRRTCVVMATHDRVDALTLSDRIAVMDGGSVVQ
                     VGTPSEVMYRPVSEAVAAFVGIENMVRGVVTGVAGGVLEVQAGRARIRALGAFPRGEE
                     VVCLVRPESVTLSTPGTHEESSARNVLHGRVVRVRNRGDVQGVFIDCGFPLVASVTKN
                     ALEELCIEEGREVTALFKATAVHVVKSARAYSGGRNT"
     gene            complement(6913..>7339)
                     /locus_tag="PLT69_02205"
     CDS             complement(6913..>7339)
                     /locus_tag="PLT69_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ13292.1"
                     /translation="GVLELLYTPTAIVIAQAVIATPIVAGISLASIQKLPEALRLQIL
                     ALGATRAQMVWMLIKEARLPLLAAVMAGFGGVISEVGASIMVGGNIKGYTRVLTPATV
                     METSRGNFDTAIALGVILLLFTFCINAALTSIQQKERPR"
BASE COUNT         1395 a         2369 c         2319 g         1256 t
ORIGIN      
        1 atcggctgcg ccttcatcgt ctctctgttt ctgtgcccag gcgcctgggc ggaacggacc
       61 gtgacggtgg cgctcggcgg agacgtcttc cacatgaagg agaagagtgc gaagggcggc
      121 ttcgaagggt atgaaatcga cctgatgaag gctgtggccc aggaggcggg attcaagatc
      181 aagttcgtgg aagtgccctg gaagtccctg ttcgacggcc tcaacgaggg gaaatacgac
      241 gcggtcatcg catccgtgaa catcacgtcc gggaggaagg aacgttacga gctctccgat
      301 ccgtacttca gcgcggcgca gctcctcgtg gtccccaggt caaaggtcaa catgaagctg
      361 agcggcagga acgtgggtgt cttcaggctc tccgccgccg ccgacaggat caggagatcc
      421 ggcgccaaca tcacctacta caccgtgcag gagacgccgc aagcattcaa ggaccttcag
      481 aaggggacga tcgacgccct gctctgcgac agccccgtgg ccatcggctg cacggcgggc
      541 tccacgtcat cctccctgtg catagcgagc ctggacatcc tcgacgcgaa agaatgcgtg
      601 gaagagtacg gcatcgtctg caggaagggc aacagggagc tcgtggagct catcaacaag
      661 ggcctggcgt ccgtgaaggc gaagaagctc gacaacgagt tcgcgctcag gtggttcaag
      721 gggaccgctg tcgcgtccac atcgcagggc tccgggtcct tcttcgcttc cgagatccag
      781 accggacccc agaactctcc ggtctcatcc aagtagcccg ccttgcccga gcccgcttag
      841 ggcacccccg gggggcgtcc cttgcccccc gtcgcgtggc gccctcaccg tcacgcgccc
      901 ggcctggggg gcctcacgca ggcgagcccg tcgatgaaat ggccgatggc gagcaggggg
      961 tgttcgaaca tcatgcgggg cccggcatac cgcatgacac gcctgacctc ttcccgcatg
     1021 cccccctgat agcaatggat ggggcatgcg gcgcaggtgg gcttcttcgc gccgtagggg
     1081 caatttcgca gacgtttctc ggcgtactcg cacaactccc ggcagcggga acacaaggcc
     1141 cctttcgacc cgtgacggtc cctgcagtac atttcgatca tgaccctgat ggtcttttcc
     1201 tcgcgcaaga gacggccttt cattctcctc accactttcg ggctcaccgc ggccaactgt
     1261 cgcagggtcc ggtatccttc acgtccaccc accctgtcaa ggtgctcacg gcaacctcct
     1321 cgccgcctgc accctgcccc cggcggtcgg tctcctctat gcatgcacaa ggggcgtgaa
     1381 aaggacggga cgatcacttc gcgtgcggca tcctccttca tgcatgcaaa caggggtgtg
     1441 atcccatgca acaagacaaa cggctttgcc gccttccccg ccccttgtcg cgtcttcttc
     1501 cccggacatg tcatgggcgg caccccgcag gcgtttgtct cacgccgtga cccgcctcca
     1561 acggcacgga ccgctccggc acgaggccgt acccgcaccg ggcatcgagg atgagagacc
     1621 cgttccgcgt gtatacaggg catcggcgca ccgctctcgt gatgcatggc caaatcgttc
     1681 caaaaggtct ataaggaatg catcaagtac atcgcttgca ggttcgataa gaatcacggg
     1741 cgaagatgaa gatcatctac ctgaccgata tccacggcga cttcgagagg atgaagtccg
     1801 tgctctcgga gacggtcgcc aacgtgtaca tcatcggcgg agacctcata gacatcccct
     1861 tctacagcat ggagacctct atcaggtacc atgagctcca gtccttcttc cacggcctcc
     1921 gcatgaagat gggccaggag gacaggctcc tggaggactt cgtggaccag ctcctggagc
     1981 agcccgacgt cccggaagac gtccaggaca aggggacgat gtaccagcag tacaccatca
     2041 gggcgcgcag ggtcatgcag cagaagtaca aggtcctcaa gaacctcgtg tccatgaaga
     2101 aggcgtccac ggtcttgttc ctgcccggca actacgacat ggacctcaag tacacggcct
     2161 tgcacgacca cgacctgcac ctccactggt acgcgatcgg cgacgtgcgc atctgcgggt
     2221 acggcggggc cgaggtgagg acgccgggca ttcccgagcg ctatatcgtg agatacctcg
     2281 agggcttgag ccccaggggc agaaagaacg ggaaaaagaa cgagatgtac tcgttcttcc
     2341 gcgtggtgaa gcctcacatc atcgtggcgc accagccggc ctacggcatc aacgacaaca
     2401 ccacggccgg cggggcgtac gggtcgccgt ccctcaggac cttctgcgac gagaacccgg
     2461 tgctcctctg cctcacgggc cacgcccacg actcgtgggg cgtgaagtac tcgggcaaga
     2521 ccctctacct gaacccctcg aacttcggcg aggtgaccgg gccgacggga gaggtgaacg
     2581 agggtgggtt cttctaccag atcgagatgg accagaagga agtcaagtcc atcgtgttca
     2641 ggaagttcca cgacggcagg atctacgacg tcgcagacta taccctcgag aacgggaccc
     2701 tcgtcgagaa gatcatcgac agggaacggt acgcggcgct cgtagaccgc aggaactacg
     2761 acatgaaagg cgttcgctac tcgcacattc ccgagataag gctttacaag gagatcaagg
     2821 acttcttcag gatgtaccag acaccggagg cggaggagcg ggttgacagg atcgaggagg
     2881 tcgcccgggt gctcgaggaa aagaccgggc gcgaggtggc catggatgtc atgggctcgg
     2941 tgaacatggg gatatccgaa cccgacagcg acatcgacct cgtcctctac gtgaggtgtt
     3001 cgtcgacctg cgtggacgac gtgaacgtgt gcgagacctc cgccaggatg caggccgagg
     3061 tgaaagggct cctcggggaa tccatcaagg cccacgtcat ggactgcata gacctcgacc
     3121 aggtggaacg gagcatcagg gagcgggatt acgagtgcga ggtgctccag cggttcgtcg
     3181 cctaccgctc catctgcagg cccatcaact accgcgtcat agcgcccata gaagacctgc
     3241 tcaactcgga catcagcttc cgcaaggagg tcgagggcag cacgcagagc tacatacgca
     3301 tcttcgcgaa cacctccgct cacgtacgct cgatcaagaa gtacgaggcg aggctgaacg
     3361 acctgggcat ccgggtcccg gaatcggtca agcgcgcgat caagcagtac ctgagggaag
     3421 actgacaggg tccgtcccct gggtgtcgca cgttcctccc gacatgccgt gaggcggcat
     3481 gacaggcgta tttacatttc cggcgagtgg gagtagaggg ggtggaaacg gtgtatgaat
     3541 ggtgcatcca ggcgtataag gggcggtttt tcccatggaa tgaggagggt cggccgggac
     3601 cgtgtaccgg cccttcacgc cgaggcattg ggggcttcgg gtttcgaggc ggtccgcgtg
     3661 tgccggcgca cctgcgggca cactgcacac ggagggaaag acacggcgcg agaccattcc
     3721 gggcgtcccg gcccgcagga tgtcacgcgg tgagcagatg aaaaacattc tctacaacct
     3781 cttcatcagg gaagtgagcc tcctgtgcta ccgcacctgc cttgactacg taccgggcgg
     3841 gtcgcgcatc ctcgacgtcg gcatcggcaa cggcgccatg atacaggagt tccacgagac
     3901 catccgttcg aagggcatat ccataacggg catcgacgtc gaccgctgct acctggacca
     3961 ctgcgagcgc cagatcagga agaactccct cgaagagtat gtgaacgtgt tccacgtgcc
     4021 agtcgagcgc ttcgagcctg aagagcccca ttccttcgac ttcgtcctct tcagcatgag
     4081 cttcatgctg ctcgacgatc ccgcgctcgt cctgaggcgc atacggcgat gggtcaggcc
     4141 ctcgggcggc gtcatgttct tccagaccat gttcgccagg cgctccctgc tcctcgacat
     4201 cgtgaagccg aggctcaggt acctcaccac cgtggaattc gggagggtcg tctacgacga
     4261 cgagttcttc gggctcctca ggagggaggg ggtgtccgtc gtcgaggacg tgaccgtggc
     4321 caaggcatgg ttcggcggga ggtaccgctt cgtggtgtgc aggcccgaga cggctgcaga
     4381 cgtgttcacc ctcccgaagg ccgagatagt cgggcccggg gacgcactcg acgccccgta
     4441 caggacttcc cgcgcctcac gcacgctcag gcggaacgcc ttccggtcgc ggtcgagcgg
     4501 caaggatgtg gagagatgac gaacccgggg cgtctggtgc acacccatgt ggttgcgccg
     4561 ctcaaggcgc tcctgaacca ggggatcacc cccgggagac tcgccgcgag catggcatgc
     4621 ggcatcaccg tcggcgtttc gcccctggtg ggcaccacca ccgccttctg caccatgctg
     4681 gcgctggccc tccgcatgaa cctgctcgcc atacagctcg gcaactggct cgccctgccg
     4741 gtgcagctcg ccctcatagc ccccttcctc atgatgggca atcgtctctt cggcgacggg
     4801 cgccatcacg actgggcatc cctggcgctc ttggtgaggg aggatgcgat gctcgccgtc
     4861 caggccgcga gcaggatggt gattcacggc gcccttgcct gggcgctgtg cgcgccggcc
     4921 gtgttcgcgg ccttgttcgc cctcctcctt cccgcattca ggatgctggg aggcctgaac
     4981 caacgatcca cgggaaagaa gggatgaggt ctcgagcgca cccccgggtg gagaccccat
     5041 gaagaaaggc caagacctct cgcggggacg cgaggaagcc atggacaaag gaagagaacc
     5101 ttgccgcaga tcgaggatcc tgctccacgt ctgctgcgca ccgtgcgcga cgtggcccgt
     5161 cgagaggctc tcccgagagg gttacgaggc cgtgctcttc ttcagcaact cgaacgtcca
     5221 ccccgaggag gagtacacga ggcggctcga cgaggcgaga aggcttgcag ggatcctggg
     5281 cctcgagctc gtcgaggacg tctacgacca cgaggcgtgg ttagatgcgg tcaggggcct
     5341 ggaggccgag ccggagcggg ggaagcgatg catgaagtgc ttcgcataca acctcggtcg
     5401 cgccgcgctg gccatgggac gcctggggct ttcccgcttc accaccacgc tcaccgtgag
     5461 caggcacaag tcgtccaggg acgtcttcgc cgccgctcgg accatgcccg gattcttggc
     5521 cgtggacttc aagaaacagg acgggtacca ccaaagcgtc gtgctcagca gagggctcaa
     5581 gctctaccgc cagggatact gcgggtgcga gttctcccgg caggcggcgc tgcgggacag
     5641 ccctgcatcg ggcaagacct gaccctcctc atgccggtcc aagagcgcgt tctggtccct
     5701 cgaggggtgg agccttcggg atgccgcccc aagaggcgtg gacgcccgcc tgcccccctc
     5761 aggcgcagag gtggaaggtt gcctctccag gaacgagcct tcacgtgttc cggcccccgg
     5821 agtacgcccg cgcgcttttg accacgtgca cggccgttgc cttgaacagg gcggtcacct
     5881 ccctgccctc ctctatgcag agctcctcga gggcgttttt cgtcacggag gcgacgagcg
     5941 ggaagccgca atcgatgaag acaccctgca cgtcccccct gttccgcacc ctgacgacgc
     6001 ggccgtgcag gacattcctc gcgctcgatt cctcgtgagt gcctggggtg gagagggtga
     6061 cgctctccgg ccgcacgagg cacaccactt cctcgcccct cgggaaggcg cccagggcgc
     6121 ggatcctcgc tcggccagcc tgcacctcga gcacgccacc ggcaaccccc gtcacgaccc
     6181 ccctcaccat gttctcgatg cccacgaagg cggcgacggc ctcgctgaca ggcctgtaca
     6241 tcacctccga gggggtaccc acctgtacca cggacccgcc gtccatgacc gcgatcctgt
     6301 cggagagcgt caatgcgtcc acccggtcgt gcgtggccat gacgacgcac gttcggcgca
     6361 gtcggatgac ccgggagagg tcctcgacga tcgattcctt ggtgggggga tcgagcgacg
     6421 agaacggttc gtccatgaag acgatctccg gctgggtggc gaatgcccgt gccaggctgg
     6481 ttctctgggc ctcgcccccc gacagcttcc tcgccgagcg gtcagagagg tgggcgatcc
     6541 cgaagagttc caggttttcc ctcaccctgt ctctcgtctc gtgtctgccc atgcccctga
     6601 tcgccagacc cgacgccacg ttcgcgtaca ccgtggagtc gaagagcagg ggttcctgga
     6661 agaccatggc tatcctgcgg cggtactcga gcatgccgcg cccgcccgcg atcttcacgc
     6721 cgccgaacct gagttccccc gagcccagct cgaggaggcc ggctaggctc aagagcagcg
     6781 tcgacttgcc ggagccgttc ggtccgatga cggacaggaa ttcgcccggg ttcagggtga
     6841 actcccggac gtcgaggaca ggggtccccc ccctgctcac cttgaggtct ctcgcctcga
     6901 ggatgggacc gctcacctcg gcctctcctt ctgctggatg gaggtgagcg cggcgttgat
     6961 gcagaaggtg aagagcagga ggatcacgcc gagcgcgatg gccgtgtcga agttgcccct
     7021 tgacgtctcc atgaccgtgg ccggggtgag cacgcgcgtg tagcccttga tgttgccgcc
     7081 caccatgatg gaggccccca cctcggagat gacccccccg aacccggcca tgaccgcggc
     7141 caagagcggc agcctggcct ccttgatgag catccagacc atctgggccc tggtcgcccc
     7201 cagcgccagg atctggagcc tcaaggcctc ggggagcttc tggatggagg cgagcgagat
     7261 gccggccacg atcggcgtgg cgatgacggc ctgggcgatg acgatggccg tgggcgtgta
     7321 gaggagctcg aggacccca
//
LOCUS       DAOSCR010000047         7309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6537_length_7309_cov_3.220982, whole genome shotgun sequence.
ACCESSION   DAOSCR010000047 DAOSCR010000000
VERSION     DAOSCR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6537_length_7309_cov_3.220982"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(704..1651)
                     /locus_tag="PLT69_02210"
     CDS             complement(704..1651)
                     /locus_tag="PLT69_02210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOJ13293.1"
                     /translation="MQTLESLKRRIKSTQDLLSVVKTMKALAAVSIRQYQRAVESLAD
                     YARNVEMGLQVVLQSRPREEMLTGTVPCTRAGAVVFGSDQGLCGQFNALVTSHALDEL
                     DRLGLKPQDRYILTVGERVKDLVTASGQDIYDNLSTPSSIAGITPMVQEMVLILEEWH
                     FGAQIQHIYLYYNHYVSGATYHPATLRLLPVDQEWLEQVRSRKWESKSLPMFTQDWQG
                     LFLSLLREYLFVSLYRAFTESLASENASRLASMQNAEKNIQEHLEEIHVAYHRQRQMT
                     ITEELLDIVAGYEAMKGDTRLGKDEEKQGRGMPGSRAAT"
     gene            complement(1638..3194)
                     /locus_tag="PLT69_02215"
     CDS             complement(1638..3194)
                     /locus_tag="PLT69_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HOJ13294.1"
                     /translation="MEERSDEGTLRRCLDDTVKRLDRVVSGTKPGLRPEEVGVVTFVG
                     RGIARLSGFDTIRSEELVRFPGNLYGLVFNVDPDEVGIIMLDPTERIRAGDIVHRTGR
                     VLDVPVGEALLGRVVDALGRPLDNMGEVRATARFPVEREAPPIMHRDPVTVPLQTGIK
                     AIDALIPIGRGQRELILGDRQIGKTTIALDTIINQHDKNVICIYCAIGKQSSDVAKSI
                     AILKEHGAMGYSVVVVASGEEVPGLQFVAPYAATAMGEYFMEKGRDVLVVYDDLTRHA
                     RAYRELSLLLRRPPGREAFPGDIFYIHSRMLERSTHLRAHYGGGSLTALPIIETEAQD
                     ISSYIPTNLISITDGQIYLSPNLFQKGILPPIDVGKSVSRVGGKAQLPAYRAVAGDLR
                     IAYSQFEELESFSRFGTRLDEDTKRRLERGWRVREILKQPQYRPIPVAEQIAALMAVT
                     SGVCDDVKVEDVGKAEMLVRTEVTKRLESVCTKIYEGSKLTMDDFNLMLSVAKSAVSS
                     MAGGQGDANP"
     gene            complement(3334..4173)
                     /locus_tag="PLT69_02220"
     CDS             complement(3334..4173)
                     /locus_tag="PLT69_02220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOJ13295.1"
                     /translation="MHIDWFVFAAQIINFLILVALLKYFLYDRIVKAMDARQAGIASR
                     MEEAERLRQEAGRHLQEMEEKKRQLDARVQEILNQAVRDAGAEKERLLALANEEAQES
                     RRRWKETLAREREAFYDRLRLTSGEIIFSTIRRILGDMADAALEEHVVDTFVGRIRGM
                     DEQAQELLRRSLDDSRSEVTLVSAFELRPDQQEKILSALGPFLSHDVTLRCEVRPELI
                     SGIELVSQGYRLAWSVQRHLETLEEAFKEALREELPEVNLENAGGEDAPAQEGSRQPH
                     QSG"
     gene            complement(4177..4458)
                     /locus_tag="PLT69_02225"
     CDS             complement(4177..4458)
                     /locus_tag="PLT69_02225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOJ13296.1"
                     /translation="MEILTVVALASIVTAGLTMAIGSIGPALGESLAAKQAVSAIAQQ
                     PDEKNTITRTLFVSLAMIESIAIYCFVISMILIFANPFWDHVIGMAKGG"
     gene            complement(4499..5200)
                     /locus_tag="PLT69_02230"
     CDS             complement(4499..5200)
                     /locus_tag="PLT69_02230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOJ13297.1"
                     /translation="MEILSLNQISPDQVVLLQWGVVRITATLLYTWVVMAILILVAIL
                     VGRKATADTAISRWQNLLEVVVSSIREQIREISQDEPDHYTAFIGTLFLFIGLSNLLL
                     IVPGYVAPTSSLTTTAALAVCVFFAVPIYGISKNGLAYFKEYFQPTFIFFPFHVMGEF
                     TRTLALMIRLFGNIMSHDKVIAILLAVTPLFFPIVMHALGLLIGMVQAYIFSILAMVY
                     IASVTQAHESHGRGP"
     gene            complement(5188..5502)
                     /locus_tag="PLT69_02235"
     CDS             complement(5188..5502)
                     /locus_tag="PLT69_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOJ13298.1"
                     /translation="MSVQASVPSMVLALVAGAVLGVFFCMHLKAQVRRIADAGGYTLS
                     NVAGYFLRIGFVVAGLLIVMNGRWERLAAALVGFIVARELFVRAAGPARFAHGRGSAW
                     KS"
     gene            complement(5499..5807)
                     /locus_tag="PLT69_02240"
     CDS             complement(5499..5807)
                     /locus_tag="PLT69_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ13299.1"
                     /translation="MKKFTDEVVKKEKRRIRGLRQQDKTVWFGLGMFGVVGWSVAIPT
                     LIGVAIGLWIDQRWPSQYSWALMLLILGVMVGCLNAWYWVRKGGIEKDEDSDGTGGPS
                     "
     gene            complement(5852..6241)
                     /locus_tag="PLT69_02245"
     CDS             complement(5852..6241)
                     /locus_tag="PLT69_02245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOJ13300.1"
                     /translation="MDCKILLPSEVFLHRQADKVVAEGTNGSFCLLPRHIDFVSALVP
                     GLLSLSAQDEQPEYFAVDQGILVKKGDEVLIATRNAIKIPSLGHLKSVVQERFRNLDD
                     KEKAARTAAARLEASIVRRFMELKRYG"
     gene            complement(6231..>7309)
                     /gene="atpD"
                     /locus_tag="PLT69_02250"
     CDS             complement(6231..>7309)
                     /gene="atpD"
                     /locus_tag="PLT69_02250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ13301.1"
                     /translation="EIEGTPKPIHQKPVPLTEQSTVSEIFTTGIKAIDVLAPLERGGK
                     AGLFGGAGVGKTVLITEMINNTVKKHHGVSIFCGIGERCREGEEIYRDMISAGVLDNT
                     VMVFGQMNEPPGARFRVGHAALTVAEYFRDEMHQDVLLMMDNIFRFVQAGSEVSGLMG
                     QLPSRLGYQPTLGTELAELEERICNTKAGAITSIQAVYVPADDFTDPSASHTFGHLSA
                     SIVLSRKRASEGLYPAVDLLQSWSKMLMPHIVGERHYTIAREIRKTLTIYEDLKDIIA
                     MLGIAELSREDQRTVYRARRLERFFTQPFFTTEQFTGLPGKMVSLEDALDGCERILND
                     EFADVPESMLYMIGSIDEVDKDGL"
BASE COUNT         1261 a         2383 c         2264 g         1401 t
ORIGIN      
        1 ctcatcccct tccccagggc acgacgccac ccacgagcag ggcctcccca ccgggcccca
       61 cgcctgatgg cctcgtgcag gccctgagac accgttcgaa gacgaacgaa cgcacgcctt
      121 gggccggtga gcgcccccaa gtgatgagag agaaccacga cgcggcaaca atgggctagc
      181 aggctgggaa gcgcctgcat acaaggggct gaccctcggc cgctgccaag ccggggtgtc
      241 gtgggccacg cgagatgagc gaggactcac gtgttgccgg ggcgggggtc ctgcgcggga
      301 gtcagagttc accctcccac gcgagacgag caggggatca cgggttgtcc gggatcttgc
      361 ccgatcgcgg attttctctc ttcacgtgct cacccgggag gaaaaggaag gggcccatgg
      421 gggacagggt ttcccccccc ccacgagacg gcccggcccc aggaacgcga agccgtaccg
      481 acgcagtgga cgtggtaacg gcgtctgctc accgggcggc ccgatcggca tggacagcac
      541 ctcgaagccc cttaagacgg ggatggaagg aacgagcgcc gtgtggccca ggggatcctt
      601 gaacgcgacc ccctaccgta aaagggaggt gagccctgcc tatcggggaa aatccgcccc
      661 actcaccgcc tttccccctg aggaccggag atgtggttgt gcatcacgtg gccgccctcg
      721 aacccggcat gccgcggcct tgcttctcct cgtccttgcc gagcctcgtg tcgcccttca
      781 tggcctcgta gccggccacg atgtccaaaa gctcctccgt gatggtcatc tggcgctgac
      841 ggtggtatgc cacgtggatc tcctcgaggt gctcctggat gttcttctcc gcgttctgca
      901 tggatgcgag cctgctcgcg ttctcgctcg cgagcgactc ggtgaacgcc cggtagagcg
      961 acacgaagag atactccctg agcaggctga gaaagagacc ctgccagtcc tgggtgaaca
     1021 tcggcaggct cttggactcc catttccgcg agcggacctg ctcgagccac tcctggtcaa
     1081 cgggcagaag cctcagggtc gccgggtggt aggtggcgcc tgacacgtag tggttgtaat
     1141 agagatagat gtgctggatc tgtgccccga agtgccactc ctcgaggatg aggaccattt
     1201 cctggaccat gggcgtgatg ccggcgatgg agctcggcgt ggagaggttg tcgtagatgt
     1261 cctggccgga tgcggtcacg agatccttca cgcgttcacc cacggtgagg atgtagcggt
     1321 cctggggctt gaggcccagc ctgtccagct cgtccagggc atgggaggtc accagcgcgt
     1381 tgaactggcc gcagaggcct tggtcggaac cgaacaccac cgctccggcg cgcgtacaag
     1441 ggaccgtacc cgtgagcatc tcctccctgg gacgtgactg caacaccacc tggagcccca
     1501 tctcgacgtt cctggcgtaa tcggcgaggg actccaccgc cctctggtac tgcctgatgc
     1561 tcacggcggc cagggccttc atggtcttga ccaccgagag gaggtcctgg gtgctcttga
     1621 tccgccgttt cagcgactca agggtttgca tcgccctgcc cccctgccat ggaggagacg
     1681 gcggacttgg ccacgctgag catgaggttg aagtcgtcca tggtgagttt cgacccctcg
     1741 tagatcttcg tgcacacgga ctccaaccgt ttcgtcacct cggtgcgcac gagcatctcg
     1801 gccttgccga catcctcgac cttcacatcg tcgcacaccc cgctcgtgac cgccatgagg
     1861 gccgcgatct gctccgccac gggtatcggc ctgtactggg gctgcttgag gatctcacgc
     1921 acccgccacc cgcgttcgag tctcctcttc gtgtcctcgt cgagccgggt accgaaccgt
     1981 gaaaagctct cgagctcctc gaactgggaa tacgcgatcc tcaggtcccc ggccaccgcc
     2041 cggtaggcgg gaagctgagc cttgccgccg acgcgggaga ccgacttccc gacgtctatg
     2101 ggaggcagga tccccttctg gaagaggttg ggcgacaggt agatctgtcc gtccgtgata
     2161 gagatgaggt tggtggggat gtaggaggag atgtcctgcg cctcggtctc gatgatgggc
     2221 agggcggtca gtgacccgcc gccgtagtgg gccctgaggt gggtggaccg ctcgagcatg
     2281 cgggagtgga tgtagaagat gtcacccggg aaggcctccc ttccgggcgg cctcctgagc
     2341 agcagggaaa gctccctgta ggcccgggcg tggcgtgtca ggtcgtcgta caccacgagc
     2401 acgtcgcggc ccttttccat gaagtactca cccatggccg tggccgcgta cggggccacg
     2461 aactggagcc cggggacctc ctctcccgag gccaccacca ccacgctgta gcccatggcc
     2521 ccgtgttcct tgagtatggc tatggatttc gccacgtccg agctctgctt gccgatggcg
     2581 cagtagatgc agatcacgtt cttgtcgtgc tggttgatga tggtgtccag cgcgatggtg
     2641 gtcttgccga tctgcctgtc accgaggatc agctccctct ggcccctccc gatgggtatc
     2701 agtgcgtcga tggccttgat gcctgtctgg agaggcaccg tgaccggatc tcggtgcatg
     2761 atggggggcg cctcgcgctc gaccgggaac cgtgccgtcg ctcgcacctc ccccatgttg
     2821 tcgagcggcc tgcccagggc gtccaccacc ctgcccagca gggcctcgcc gaccggcacg
     2881 tcaaggaccc tgcccgtgcg gtgcacgatg tcgcccgccc tgatcctctc tgtgggatcc
     2941 agcatgatga tgcccacctc gtccgggtcc acgttgaaca cgagcccgta gagattcccg
     3001 gggaaacgaa cgagctcttc cgaccggatg gtgtcgaacc cgctcaggcg ggcgataccc
     3061 cttcccacga aggtgaccac ccccacttcc tcgggcctga ggccgggctt cgtgccagag
     3121 accaccctgt cgagcctctt gaccgtgtcg tcgaggcacc tcctcagcgt gccctcatcg
     3181 gatctctctt ccattgcggc cccccttcac gagcctttca tgggcggcgt cctgtaagcc
     3241 ggtgtacttg caggttctct cgtgcccatc ctcccgcacg cccacgtgat atcgtccgac
     3301 cctgtgcctt ctcaccctgc agcccccgga gcctcacccg ctctggtgcg gctgcctcga
     3361 tccctcctgg gccggcgcat cttccccccc ggcattctcg aggttcacct ccggcaactc
     3421 ttccctgagt gcttccttga acgcctcctc gagggtctcg aggtgcctct gcacgctcca
     3481 ggcaagccgg tatccctgcg agacgagctc gatgccggag atgagctcag gcctcacctc
     3541 gcatctcaag gtcacgtcgt gagacagaaa cggcccgagc gccgagagga tcttttcctg
     3601 ttggtcaggc ctcagctcga aggcgctcac gagggtgacc tcactgcggg agtcgtccag
     3661 ggatctcctg aggagttcct gggcctgttc atccatgccc ctgatcctgc ccacgaacgt
     3721 atccaccacg tgctcttcca gcgcggcgtc cgccatgtcg cccaagatgc gcctgatcgt
     3781 cgagaagatg atctcccccg aggtgaggcg cagcctgtcg tagaaggcct cgcgctccct
     3841 ggcgagcgtc tctttccatc gtctcctgct ctcctgggcc tcctcgttcg cgagcgcgag
     3901 gagcctctcc ttctccgcgc ctgcatccct gaccgcctga ttgaggatct cctgcacccg
     3961 ggcgtcgagc tggcgcttct tttcctccat ctcctgcagg tgcctgcccg cctcctgcct
     4021 gagtcgctcc gcctcttcca tgcgcgaggc gatgcctgcc tgcctcgcat ccatggcctt
     4081 cacgatgcgg tcgtagagga agtacttcag cagcgccacc aggatgagga agtttatgat
     4141 ctgggcggcg aagacgaacc agtcgatgtg catccatcat ccccctttgg ccatgccgat
     4201 gacgtggtcc cagaacgggt tcgcgaagat caggatcatg gagatgacga agcagtagat
     4261 ggcgatggac tcgatcatgg cgaggctcac gaacagggtg cgggtgatgg tgttcttctc
     4321 gtccggctgc tgcgcgatgg cgcttaccgc ctgcttcgcc gccaggctct cgcccaacgc
     4381 cggaccgatg gaaccgatcg ccatggtaag ccctgctgtg acgatggatg ctagcgctac
     4441 aacagtgaga atttccatgt cataccttcc tttctcagta gagtgaggaa cgcatatctc
     4501 agggcccgcg gccgtgagac tcgtgggcct gggttaccga tgcgatgtac accatggcca
     4561 ggatggagaa gatgtacgcc tggaccatgc ctatgaggag cccgagcgcg tgcatgacga
     4621 tggggaagaa gagaggggtc acggcaagga ggatcgctat caccttgtcg tggctcatga
     4681 tgttgccgaa caacctgatc atgagtgcca gggtcctcgt gaactcgccc atcacgtgga
     4741 aggggaagaa gatgaaggtg ggctggaagt actccttgaa gtacgccagg ccgttcttgg
     4801 atatcccgta gatgggcaca gcgaaaaaga cgcacacggc cagcgccgcg gtggtggtga
     4861 gggacgaggt gggtgccacg tagccgggca cgatgaggag gagattggaa agaccgatga
     4921 acaggaacag cgtcccgatg aaggcggtgt agtggtcggg ttcgtcctgg ctgatctccc
     4981 ggatctgctc gcggatcgag gacacgacga cctcgaggag gttctgccag cgggaaatgg
     5041 ccgtatcggc ggtggccttc ctccccacga gaatcgcgac gaggatgagg atggccatga
     5101 cgacccaggt gtagaggagg gtcgccgtga tcctgacgac cccccactgg aggagtacca
     5161 cctggtcagg gctgatctgg ttcaggctca ggatttccat gccgagcccc tcccgtgggc
     5221 gaaccgtgcc ggtcctgcgg cgcgcacaaa caactcacgc gccacgatga acccgacaag
     5281 cgcggctgcc aggcgctccc acctgccgtt catgacgatg agcaaccccg ccacgacgaa
     5341 accgatcctc aggaagtagc ctgcgacgtt cgacagcgta tatcccccgg catcagcgat
     5401 ccttcgcacc tgggccttga ggtgcatgca gaagaagaca ccgaggaccg cccctgcgac
     5461 caacgcgagg accatggacg ggacgctcgc ctgcacgctc acgacggacc ccctgtaccg
     5521 tcactgtctt cgtctttctc gatcccgccc tttcgcaccc aataccacgc gttcaggcac
     5581 ccgaccatga cgccgaggat gagcagcatc agggcccacg agtactgcga cggccagcgc
     5641 tggtcgatcc agaggccgat cgcgacgccg atgagcgtgg ggatggcgac cgaccaaccc
     5701 acgacgccga acatgcccag gccgaaccag accgtcttgt cctgctgcct gaggcccctt
     5761 atcctgcgct tttctttctt gaccacctcg tcggtgaact tcttcatgat atcttccctg
     5821 cggaggtgat ccttcctggg ctgcctgtcc atcacccgta cctcttgagt tccatgaacc
     5881 gcctcacgat gctcgcttcc agccttgcgg cggcagtgcg cgccgccttc tccttgtcgt
     5941 ccaggttcct gaaccgctcc tgaacgaccg acttgaggtg ccccagactc gggatcttga
     6001 tcgcgttgcg cgtagcgatg agcacctcgt cgcccttctt gacgaggatg ccctggtcga
     6061 cggcgaagta ctccggctgt tcgtcctgtg cggacaggct caggagcccc ggcaccaagg
     6121 ccgagacgaa atcgatatgc ctcggcagga ggcagaacga cccgttcgtc ccctcggcca
     6181 ccaccttgtc cgcctgcctg tggaggaaga cctcggacgg gagcaggatc ttacagtcca
     6241 tccttgtcga cctcgtctat cgaaccgatc atgtacagca tggattccgg cacgtccgcg
     6301 aactcgtcgt tgaggatgcg ctcgcacccg tcgagcgcgt cctcgagcga taccatcttc
     6361 ccaggcagac ccgtgaactg ctcggtggtg aagaagggct gggtgaagaa ccgctcgagc
     6421 cttctggccc tgtacacggt ccgctggtcc tcgcgcgaga gctcggctat gccgagcatg
     6481 gcgatgatgt ccttgaggtc ctcgtagatg gtgagcgtct tcctgatctc cctcgctatc
     6541 gtgtagtgcc tctcgccgac gatgtggggc atgagcatct tcgaccacga ctggaggagg
     6601 tccacggccg ggtagaggcc ctcgctcgcc cgcttccgcg agagcacgat ggacgccgag
     6661 aggtggccga aggtgtgaga ggccgaaggg tcggtgaagt cgtccgcagg gacgtacaca
     6721 gcctggatgg aggttatggc gcctgccttg gtgttgcaga tccgctcctc gagctcggcc
     6781 agctcggtcc cgagggtcgg ctggtagccg agcctcgacg gcagctggcc catgaggccg
     6841 gagacctccg agccggcctg gacgaacctg aagatgttgt ccatcatgag gaggacgtcc
     6901 tggtgcatct cgtcgcggaa atactcggcc acggtgagag ccgcgtgacc caccctgaag
     6961 cgggcgcccg ggggctcgtt catctggccg aagaccatga ccgtgttgtc gagcacgccg
     7021 gcggatatca tgtcgcggta tatttcctct ccctcgcggc agcgttctcc tatgccgcag
     7081 aagatgctca cgccgtggtg tttcttcacg gtgttgttga tcatctcggt gatgagcacg
     7141 gtcttgccga cacccgcacc gccgaagaga cccgccttcc ccccgcgttc caacggggcc
     7201 agcacgtcga tggccttgat gccggttgtg aagatctccg agacggtcga ctgttccgtg
     7261 agcgggaccg gcttctggtg gatgggtttc ggcgttccct cgatctccc
//
LOCUS       DAOSCR010000048         7252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6598_length_7252_cov_2.950535, whole genome shotgun sequence.
ACCESSION   DAOSCR010000048 DAOSCR010000000
VERSION     DAOSCR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6598_length_7252_cov_2.950535"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="selD"
                     /locus_tag="PLT69_02255"
     CDS             <1..747
                     /gene="selD"
                     /locus_tag="PLT69_02255"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOJ13302.1"
                     /translation="MNIICFPAYDMDISILEEILRGGSDKMAEAGVLLVGGHSVEDRE
                     IKYGLSVTGVVHPEKVLRNTGARAGDALVLTKPLGTGIIATAVKAGLASEPAERRAVE
                     VMKTLNSAGASAMSAFEVHACTDVTGFGLLGHACEMIEKTDVGLRISASRVPLIEGAL
                     EYASMGLIPGGAYRNRDFRSPMVDVDPSLPDDTTVVLFDPQTSGGLLVSVDARVAEKL
                     IERLHDLGMWDASLIGEFTSDHPGRIVVVP"
     gene            1403..2194
                     /locus_tag="PLT69_02260"
     CDS             1403..2194
                     /locus_tag="PLT69_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ13303.1"
                     /translation="MKRNVIMVLVLFLAVMAGSTAFGADDSWERVKKAGKITIGIDDA
                     FPPMEFRNDKNELVGFDIDASKELAKRLGIKVEHIPTVWDTVILSLKAKKFDIVWSGM
                     SITAEREKEIAFTRPYIMEKQVVVVKAGNKKIRSLKDLGADTVVGVQLGSTSEEALAK
                     LNRKFKEIKKYDKNTDAFMDLSIGRIDALAVDELVGRYYLSQKPGKYTVLKEELTSEP
                     IGIGLRKEDVALKEKIQATLDGMFKDGTMKKISIKWFGDDITTWK"
     gene            2202..3230
                     /locus_tag="PLT69_02265"
     CDS             2202..3230
                     /locus_tag="PLT69_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ13304.1"
                     /translation="MRGRPWVAALTLGLSIIVAAVPLMGADSPGTPDGYALLAEASRL
                     VQEERLAEAVAVLRKIPAPAPGEDAGLFVRSRIDAAKILLGLGDLDGASASCHEVLAL
                     LPSNLEAKNLLNEIRDARTPRWVKFLRDMKTYLPTLLRATGMTLLLVLATMLISPVAG
                     LAIALGRISRMRVVSGVFWCYIWVFRGTPLLLQLFFIYYGLPSLGVTLEPLTAAILGL
                     GLNYSAYLAEIIRGAIESIDAGQMEAAKALGMTYWQAMRRIIIPQTYRRLVPPVGNEF
                     IALIKDTALVSTIAMVELMRAANQIYSATFNVFILFQAAIVYLVLTSFFTVAFRRLED
                     ALGVYEIR"
     gene            3227..3985
                     /locus_tag="PLT69_02270"
     CDS             3227..3985
                     /locus_tag="PLT69_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ13305.1"
                     /translation="MIELRGIVKRYGRLVALDHVDLSIAEGERIVIIGPSGSGKTTLL
                     RTINFLETIDEGEILFDGRPAGYRQDRKGRLVLEKQRVICALRSQIGMVFQHFHLFPH
                     MTVLENVMEGPVTVKRTPREEARALALDLIGKVGLMDKKDVYPAFLSGGQKQRVAIAR
                     ALAMRPRLMLFDEPTSALDPELVGEVFTAIKALAAEGMTMVIVTHQMGFAREMADRVI
                     FMDNARFIYEGSPVEVFSGEMANPRVKAFLESVL"
     gene            4333..>4628
                     /locus_tag="PLT69_02275"
     CDS             4333..>4628
                     /locus_tag="PLT69_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U34"
                     /protein_id="HOJ13306.1"
                     /translation="MCDTFAVGPSVTKDGTTIFAKNSDREPDEAHIVTLAPRAEHRAG
                     ETLRCTYIEIPQARRTHAVVLAKPAWIWGAEMGVNEKGVVIGNEALIMKQKPERT"
     assembly_gap    4629..4638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4639..5619
                     /locus_tag="PLT69_02280"
     CDS             <4639..5619
                     /locus_tag="PLT69_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13307.1"
                     /translation="TPGLIGMDLVRLGLERASSARQACEVIVDLLARYGQGGACGYRD
                     KSFVYMNSFLVADRGELLVLETIGRDYAVRPARDHAVISNAPSIASNWELSSLRPGTD
                     LVKARDPLVTFFAGSARRKALNEQGIRRAWGRFTVETAFALLRSHAPGRPWPGFNADV
                     CMHAADPLIRRSQTTGSLVVELHPQDRFRIFVTATSAPCLSPFKPFLPAAPYEEASRA
                     GAVYTPGSFWWEHEVLHLSAMLRPHTFRESITREVEALEGSWTGEYPAHGWDEATDDL
                     VRVSHGAFEEAKKARSRWVSRLEGVKACPFGMRGFYLRRVARRNRVPLRV"
     gene            6275..6919
                     /locus_tag="PLT69_02285"
     CDS             6275..6919
                     /locus_tag="PLT69_02285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ13308.1"
                     /translation="MQDRYRFIGPLYDLLAAIFSLGKIDRCKCAHHDRIASGTKVLFA
                     GVGQGVDAIEAAKRGGDVTVVDISQTMLSIFKKRTDKMGLASPIRIVHSDIFAFDEYE
                     RYDMVFANFFLNVFPLDTVLRLERHLASLAKPGGCVVVGDFCLPSGGAAARFFQNLYW
                     YIADVVFYLAARNAIHPVYDYCTHLKDLGLAIEQIKYFRVLFDDRYYSILARKP"
BASE COUNT         1282 a         2229 c         2478 g         1253 t
ORIGIN      
        1 atgaacatca tctgctttcc cgcatacgac atggacatct cgatcctcga ggagatcctc
       61 aggggcgggt cggacaagat ggccgaggcg ggggtgctcc tggtgggcgg ccacagcgtg
      121 gaggacaggg agatcaagta cggcctgtcc gtgaccggcg tggtgcaccc cgagaaggtc
      181 ctgaggaaca ccggggcgcg cgcgggcgat gcgctcgtgc tcaccaagcc cctcggcacc
      241 gggatcatcg ccaccgccgt gaaggccggc ctcgcgagcg aacctgcaga gcgcagggcc
      301 gtggaggtca tgaagacgct caacagcgcg ggtgcatcgg ccatgtccgc cttcgaggtc
      361 catgcctgca cggacgtgac cgggttcggg ctgctgggac acgcctgcga gatgatcgag
      421 aagacggacg tggggctgag gatcagcgca tcaagggtgc ccttgatcga gggcgcgctc
      481 gagtacgcct ccatggggct catacccggc ggtgcgtaca ggaacaggga tttcaggtcc
      541 cccatggtgg atgtcgaccc ctccctgccc gacgacacga ccgtggtcct cttcgacccc
      601 cagacctcgg gaggcctgct cgtgagcgtt gatgcgaggg tagcggagaa actcatcgag
      661 cgccttcacg acctggggat gtgggatgcc tccctgatcg gcgagttcac gtcagatcac
      721 cccggacgga tcgtggtcgt cccgtgatcc cgccacgcgg ggtgacggct gtttcctcgc
      781 cattccaggt catgtggtcc ccgccgttcg tgatacggcc gccctgccgg cgaaagagag
      841 ggccttcccc ttgtgcatgc ccgtgacgtc tgggacggcg atcgtcgtgt gcccgcaccc
      901 tttccctcgg cccggcaagc cctcgagtcg atgtgtccac ggtccgggcc tcgtctcgtc
      961 gtgcgcacga ccccgcctgt cggatggagg aaacgtgcga gaggggttgt cgacaacaca
     1021 cggggtggac gtgtcctgaa tgcatgtcgc atgcaggggt gcggccatcg atgtccagcc
     1081 ggcgagggct catcggtcgg caccttgagc cccgggacac tgcccgggtg gaatcaccct
     1141 gtggcgggtc gccgaacggg cccggaagcg gccgcacgcc cgcgcaggga cattgggacg
     1201 gccccgggtg tcctgcccgc cccccgtttg tgcctcggac ccgatctgtg actgcgctgg
     1261 cccaaaccga cagtgcgagt cgggacgggt gccgtgtttg tctccggcag cccgtcggtc
     1321 ttgcccctgc tgcttgtgtt tgccgttggt gttcgattac aatgacaagg ttgcacaata
     1381 cagaacgaga ggggggtagg ttatgaagag gaacgtcatc atggtgttgg tgctgttctt
     1441 ggcggtcatg gcgggatcta cggccttcgg cgccgacgac tcgtgggaga gggtgaagaa
     1501 ggccgggaag atcaccatcg ggatagacga cgcgttcccg cccatggagt tccgcaatga
     1561 caagaacgag ctcgtggggt tcgacatcga cgcgtccaag gagctcgcaa agaggctcgg
     1621 catcaaggtc gagcacatcc ccacggtctg ggacacggtg atcctctcgc tcaaggcgaa
     1681 gaagttcgac atcgtgtggt ccggcatgtc catcacggcc gagcgtgaga aggagatcgc
     1741 cttcacgagg ccctacatca tggagaagca ggtggtggtg gtcaaggccg gcaacaagaa
     1801 gatccgctcg ctcaaggacc tgggcgcgga cacggtcgtg ggcgtccagc tcggctcgac
     1861 ctccgaggag gccctggcga agctgaacag gaagttcaag gagatcaaga agtacgacaa
     1921 gaacacggac gccttcatgg acctgtccat cgggaggatc gacgcgttgg ccgtggacga
     1981 gctcgtggga aggtactacc tctcgcagaa gccgggcaag tacacggtgc tcaaggagga
     2041 actcacctcc gagcccatcg gcataggcct caggaaggag gacgtggccc tcaaggagaa
     2101 gatccaggcg accctcgacg gcatgttcaa ggacggcacc atgaagaaga tctccatcaa
     2161 gtggttcggg gatgacatca cgacctggaa gtgagctgca gatgcgcggg aggccctggg
     2221 tcgcggccct gaccctgggc ctctcgatca ttgtcgcggc cgtacccctc atgggggccg
     2281 attccccggg aacccccgac gggtacgcgc tcctcgcgga ggcctcgcgc ctcgtgcagg
     2341 aggagaggct tgccgaggcg gtggccgtcc tcaggaagat accggccccc gccccaggcg
     2401 aggacgcggg gctcttcgtg cgctcgcgca tcgacgcggc gaagatcctc ttggggctcg
     2461 gagacctgga tggcgcatcc gcctcgtgtc acgaggtcct ggccctgttg cccagcaacc
     2521 tcgaggccaa gaacctcttg aacgagatcc gggatgcgag gacacccagg tgggtcaagt
     2581 tcctgcgtga catgaagacc tacctgccga cgctcctcag ggcgaccggc atgacgctcc
     2641 tcctcgtcct tgcgaccatg ctcatatcgc ccgtggccgg gcttgccatc gcgctcggca
     2701 ggatcagcag gatgagggtc gtctccgggg tcttctggtg ctacatctgg gtgttcaggg
     2761 gcacccccct gctcctccag ctcttcttca tctactacgg cctgccctcg ctgggtgtca
     2821 ccctcgagcc gttgaccgcg gcgatcctcg gtctcgggct caactactcc gcctacctcg
     2881 cggagatcat caggggggca atcgagagca tcgacgcggg gcagatggag gcggccaagg
     2941 cgctcggcat gacctactgg caggccatgc gcaggatcat catcccgcag acctacagac
     3001 gcctcgtgcc gcctgtggga aacgagttca tcgcgctcat caaggacacg gccctggtct
     3061 cgaccatcgc catggtcgag ctcatgcggg ccgccaacca gatctacagc gccacgttca
     3121 acgtgttcat cctgttccag gccgcgatcg tgtatctcgt gctcacgagc ttcttcaccg
     3181 tggcgttcag gcggctcgag gacgcactgg gggtctacga gatcagatga tagagctcag
     3241 ggggatcgtg aagcgctacg ggaggctcgt ggccttggac cacgtggacc tctccattgc
     3301 cgagggggag cgaatcgtca tcataggccc ttccgggtcg ggcaagacga ccctgctcag
     3361 gaccatcaac ttcctcgaga ccatcgacga gggcgagatc ctgttcgacg ggaggcccgc
     3421 gggctacagg caggaccgca agggcaggct cgtcctcgag aagcagcggg tcatctgcgc
     3481 cctcaggtcc cagatcggca tggtcttcca gcacttccac ctcttcccgc acatgaccgt
     3541 gctcgaaaac gtcatggagg ggcctgtcac cgtcaagagg acgcccaggg aagaggcgcg
     3601 ggcgctggcg ctcgacctca taggcaaggt gggtctcatg gacaagaagg acgtctaccc
     3661 ggccttcctc tccggggggc agaagcagcg ggtggcgata gcacgtgcgc tcgccatgag
     3721 gccgaggctc atgctcttcg acgagccgac ctcggccttg gacccggagc tcgtggggga
     3781 ggtgttcacc gccatcaagg ccctggcggc ggaggggatg accatggtga tcgtaaccca
     3841 ccagatgggc ttcgcccgag agatggcgga ccgggtcatc ttcatggaca acgcgaggtt
     3901 catctacgag ggaagccccg tggaggtctt ctcgggcgag atggcgaacc cgcgggtcaa
     3961 ggccttcctg gagagcgtgc tctagggttc ggggccgggg tggcgaacgt catggcgcca
     4021 tggagaggcg tcctcatgat cgagcttcgc agggccgttc atgtcccgga aaagcggcgt
     4081 cagtgggttg catggagagg cttttcatga tcgatcgttg cagggggttg tcgggccttg
     4141 gactctctca ggccctgcgg ccgggcgtcg gccgaggcat gaagacccag gcgccggccg
     4201 ggggagactt cggccggcca tgtgaccggc ccatccagtc ggtgatcccc ggctgcgggg
     4261 tcagggtgcg cccggcgacc aggggaggcc ggggcctggg gtgggggaac cggtacggga
     4321 ggggggatac ccatgtgcga taccttcgct gtcgggccca gtgtgaccaa agacgggacg
     4381 accattttcg ccaagaactc cgaccgtgag cccgacgagg cgcatatcgt cacgctcgcg
     4441 ccccgggcgg aacaccgcgc tggcgagacc ctgaggtgca cctacatcga gataccacag
     4501 gcgcgccgca cgcacgcggt ggtgctcgcc aagccggcct ggatctgggg cgcggagatg
     4561 ggggtcaacg agaagggggt cgtcataggc aacgaggcgc ttatcatgaa gcagaagcct
     4621 gaacgcacnn nnnnnnnnac cccgggactc ataggcatgg acctggtgag gctcggcctc
     4681 gagcgcgcct catccgcccg ccaggcctgc gaggtcatcg tggatctcct cgcgaggtac
     4741 gggcagggag gtgcgtgcgg gtaccgcgac aagtccttcg tctacatgaa ctctttcctc
     4801 gtggccgacc ggggggagct gctggtgctc gagacgatcg ggagggacta tgccgtgagg
     4861 ccggcgcgtg accacgcggt catatcgaac gccccctcga tcgcctcgaa ctgggaactc
     4921 tcttccctgc ggcccgggac ggatctcgtc aaggcccggg accctctcgt caccttcttc
     4981 gcggggagcg cgcgcagaaa ggcgctcaac gagcagggca tacgcagggc gtggggccgc
     5041 tttaccgtgg agaccgcctt cgcgctcctg aggagccatg cgccgggcag gccctggccc
     5101 gggttcaacg cggacgtgtg catgcacgcc gcggaccctc tcatacggag gagccagacc
     5161 accgggtctc tcgtcgtcga gcttcacccc caagaccggt tcaggatctt cgtgacggcg
     5221 acaagcgcgc cttgcctgtc gccgttcaag cccttcctgc cggcggcgcc ttacgaggag
     5281 gcctcgcgcg caggtgcggt atacaccccg gggtcgttct ggtgggaaca cgaggtgttg
     5341 cacctgagcg ccatgctgag gccccacacc ttcagggaat cgatcacccg cgaggttgag
     5401 gcgctcgagg ggtcgtggac cggggagtac cctgctcacg ggtgggacga ggcgacggat
     5461 gatctcgtga gggtatcgca cggggcgttc gaggaggcca agaaggcgag atcccgttgg
     5521 gtctcccggc tcgagggggt gaaggcctgt ccgtttggca tgagggggtt ctatctgagg
     5581 cgcgtggcca ggaggaaccg ggtgcctctc agggtgtagc acgtgccgtg gccagggacg
     5641 gggtgatcgt gtgagctcga acaacggcat cccttcactg cataagcggt gtgccgagcg
     5701 ccaggggagg gtcccgtccc accgggaggc ccgaggtcgc ctgtcgcgct ccaagacacg
     5761 gtgacggccc cgaaagatac ctgtacgtcg gtggtgggcg atcgctcaac atctctgtgc
     5821 gccccatgcc ggtcgccggc gtttcatggg accggagaac cccctgccaa gggagaacgc
     5881 acgccttcag gacgctcgtc ccaagaccca tgacagtcac ccctgccggc atggctcggg
     5941 tggcctgcct ggcgatggga gaaacaacgc gccctcgagc tcgtgattga ggagagcgga
     6001 caccggtgtc gccacggagg ggaggggact gcatacccct ctgaccgggt cgtgcggtcg
     6061 tcaggcatgc cgacccgctc tcgtgatagc ttcgtctcgg gtcgaggccg cgttgccgtg
     6121 tcccgggagg ttgcaaaagg gaccctgctg gtatatggag caaaagacgg ctgcgtgggg
     6181 gttcatgtat tccctcggtg atgtggaagc cgtccggccg acagggagct gtcgagccca
     6241 cagccgggga ttccgggttg gcgggaggtg atcgatgcag gacaggtaca ggttcatagg
     6301 ccctctctat gacctgctcg ctgcgatctt cagcctcggg aagatcgaca ggtgcaagtg
     6361 cgcccaccac gaccgcatag cgagcggcac gaaggtgctg tttgcaggcg tggggcaggg
     6421 ggtcgacgcc atcgaggcgg caaaaagggg cggcgatgtc acggtggtcg acatctctca
     6481 gaccatgctc tccatattca agaagaggac ggacaagatg ggattggcga gtcccatcag
     6541 gatcgtccac tccgacatct tcgccttcga cgagtacgaa cggtacgaca tggtgtttgc
     6601 gaacttcttc ctcaacgtct ttcccctgga cacggtgctc aggctcgaga ggcacctcgc
     6661 gagcctcgcg aaacccgggg ggtgcgtggt ggtgggtgac ttctgcctgc cctcgggtgg
     6721 ggctgcagcc aggttcttcc agaacctcta ttggtacata gccgacgtgg tcttctacct
     6781 cgcggcgcgt aacgccatac accccgtgta cgactactgc acccatctga aggacctggg
     6841 ccttgccata gagcaaatca agtacttccg cgtcctcttc gacgacaggt actactccat
     6901 cctcgcaagg aaaccctgag acgcgcttcc ttggtttctt tgcggcatga tgggggtctc
     6961 acggtgagcc gggcgggtgg gccgaggccc tgcactctcc ccatgggagc gcaggcctgc
     7021 atgcctcctt tggcttgaac tgctctccca gggaagtttc ccccatggaa ggacagggcc
     7081 tcccatgacc tgcaaggacg aggaaggggg gcgaggggcc cgaggagacc aggctcttcg
     7141 ccaggaggcc cggctggtcg cgtaccggtc gctccgtatc atgatcccgt cccaggaatg
     7201 gtgaggtcgt gcgtcggtcg gccggcgcgg cacgcgtgcc ggtcgcacca tg
//
LOCUS       DAOSCR010000049         7204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6655_length_7204_cov_3.463002, whole genome shotgun sequence.
ACCESSION   DAOSCR010000049 DAOSCR010000000
VERSION     DAOSCR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_7204_cov_3.463002"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..985
                     /locus_tag="PLT69_02290"
     CDS             467..985
                     /locus_tag="PLT69_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOJ13309.1"
                     /translation="MPLYALGDKAPLIGADTWVAPSAEIIGDVTIGERCWIGPCAVIR
                     GDFGSIVIGDDTAIEDGVVVHTPAMVTIGSFVTIGHLAMIHGASVGDYAVIGMNSTLG
                     DNARVGEWAIVAEHSLVKKNQVVPPGKLYAGVPAVEKGEVTQVHREVMIAGKKLYRDL
                     AGRYREGLRRIG"
     gene            1059..1682
                     /locus_tag="PLT69_02295"
     CDS             1059..1682
                     /locus_tag="PLT69_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13310.1"
                     /translation="MNHPEATAMALSILRSGNPFQWYVITLLALTLYVYVNEYSKKNY
                     RGIAAGLSLYMVHWFVEIGNALIQHFSGHALWTVPTGTAFLILVGVGIEISLMFAVSG
                     LILSKLLPEDPGMKVLGINNRLLFAVANAAFYSVFEIFLVKTPTFVWVWPFWGAFPVF
                     VTVYIPFFLAAFYAYDWKPRTQKLFIGTLFAINAASLVVFAGILKWI"
     gene            complement(1998..2558)
                     /locus_tag="PLT69_02300"
     CDS             complement(1998..2558)
                     /locus_tag="PLT69_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOJ13311.1"
                     /translation="MAGAGAPSPLSSAGLSREEIYQVGKLKPIDSVLKVKVGDVAPDF
                     SLRSVDGKTVSLRDYRGKKNVMISFVPAAWTPVCSDQWPGYNLAREEFEAMDAVVIGI
                     SVDNVPTLYAWTKSMGGLWFPVLSDFWPHGKTADVYGVLRSDGTAERAIFIVDKTGVI
                     RALDVHDINERPPLEWIMTELERLKD"
     gene            complement(2624..3178)
                     /locus_tag="PLT69_02305"
     CDS             complement(2624..3178)
                     /locus_tag="PLT69_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOJ13312.1"
                     /translation="MCKIAALAFSLLFALVQPCFGATPDGTAVETVTLAVPADAAERL
                     YLGIKATAGRFALSDIAGDILIVEIFSMYCPHCQRHAPKANELFAIIEADGKLKGRVK
                     LIGIGVGNSAYEVGFFKKKYKIPFPLFDDTGSVLLGRLEGIRTPTFFAFKKTGGRFKA
                     FFVEQGPHDDPRGFLDRVLMALPK"
     gene            complement(3250..3825)
                     /locus_tag="PLT69_02310"
     CDS             complement(3250..3825)
                     /locus_tag="PLT69_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ13313.1"
                     /translation="MATLKGSKTEKNLLASFAGESQARMRYTYFASQARKDGYMQIAD
                     IFEETANQEKEHAKRFFKFLEGGEVEITASFPAGVIGSTLENLKAAAAGEHHEQAEMY
                     PGFARVAREEGFEAIAKVWEAVSVAERQHEKRYRELAENIAQGRVFKRDKAVVWRCRN
                     CGYLHEGTEAPASCPACAHPRDYFEILGENW"
     gene            complement(3839..4267)
                     /locus_tag="PLT69_02315"
     CDS             complement(3839..4267)
                     /locus_tag="PLT69_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ13314.1"
                     /translation="MTKQRALILEELRKVRTHPTAADVYEAVRRCMPRISLGTVYRNL
                     DLLSREGLVRRIDLGTGKKRYDAFVEDHSHIRCSSCGRVDDVPLHRADGVERTTGYEV
                     QSCEIDFVGLCPECRKDSAAASRERSSRKNDLAGPEKTRK"
     gene            4723..5433
                     /locus_tag="PLT69_02320"
     CDS             4723..5433
                     /locus_tag="PLT69_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOJ13315.1"
                     /translation="MLENLSEALDLATKTSTLTPIVLAYLAGALVSFTPCVYPLIPVT
                     VSYIGAQESAGRATGFILSLAYVLGTSVVYTAIGLAAAFSGSFFGSFQTNPWVTFTVA
                     NVFLLMGCAMLEFFVLPIPSFLRAQLFSPKRRGIAGALLLGLASGLVMSPCSAPVLTV
                     LLGYVASRQNTLLGSLMLFSFSLGMGTLLIILGTFTGLLRSIPRSGPWLVYVQKGFGL
                     VFVAMAEYYLVKTGIFLM"
     gene            5449..5976
                     /locus_tag="PLT69_02325"
     CDS             5449..5976
                     /locus_tag="PLT69_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOJ13316.1"
                     /translation="MKKTPLVATSGGLAVLFAGLLMVLYAHPSCAAEPPAWPAPQSKA
                     AYAPIFVLRDIENRAVSLRDFRGKVVLVYFTTTWCPYCKRDIPRLKNLYASMRGKPFE
                     ILAVYVNESTSRVRAFAEKYELPYRVLVDTDAHVARLYGVRGVPMRVVVDKAGKVGCY
                     QCPSPEDAIGELLRR"
     gene            complement(6343..>7204)
                     /locus_tag="PLT69_02330"
     CDS             complement(6343..>7204)
                     /locus_tag="PLT69_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOJ13317.1"
                     /translation="VNAAFLAMASANGLSCAIANPESEEVMSVKHAVDVLRGSDRDAH
                     AYIAKFGAVVPEKPQPRTHESPASLVARAILEGDRERIVELVGMALDKGEEPGRLVDE
                     VMIPAIREVGRLYESKVYFLPQLIASAEAMKLAVERIEPLLDRGGASAATKGVVVLAT
                     VRGDIHDIGKNIVGLMLRNNGFTVEDLGKDVPAERIAEAAREKGAHIVGLSALMTTTM
                     AGMEDTVRYLRNQGLRCAVMVGGAVVTGDFARAIGAAYAKDGVEAVRVAESIMASLVK
                     GTEGVHGGSR"
BASE COUNT         1224 a         2391 c         2173 g         1416 t
ORIGIN      
        1 ggtacggcgt ttctgtgcag ggcgccggtt cacagggggt ctcgatccta gccgtcgaaa
       61 acgcccacga ggcggttttc gcgctccgag ggcctctcca cccagggggc accgtcacga
      121 ggatcgcgcc cctttcccag gacgaagacc ttctccctgt gccccacggt gcatatcctc
      181 ggctgccggc ctctcgtcac ggcaggaacc gggcgcgtgc ccggcatgtg acccgttttg
      241 tcgcccgctt cggttgtctc gtttgagagc cggggctttc atggtaagat gagcgtgcac
      301 tggacaacag gagggggcgc ccgctaggcg aaaaccgggc gtgggtaccg tcgagccttc
      361 agggttcccc catgcggcca tggatgacgg cacggttcca cgcgggcttg ggggacgcgg
      421 ggttcaagcg atacggcacg tcgacgaaag ggggaggggg gacgttatgc ctctgtatgc
      481 ccttggagac aaggcgccgc tcatcggcgc ggacacgtgg gtggcgccga gcgccgagat
      541 cataggcgac gtcaccatag gcgaaagatg ctggataggc ccgtgcgcgg tgatccgcgg
      601 cgacttcggg tccatcgtga tcggcgacga cacggccatc gaggacggcg tggtcgttca
      661 cacgccggcg atggtcacga tcggttcgtt cgtgaccatc gggcacctgg ccatgataca
      721 cggcgcatcg gtgggcgact acgcggtcat aggcatgaac tccacgctgg gggacaacgc
      781 ccgggtgggc gaatgggcca tcgtggcaga acactcgctg gtcaagaaaa accaggtcgt
      841 tccccccggc aagctgtatg ccggcgtgcc ggccgtggag aagggggagg tcacacaggt
      901 ccaccgcgag gtgatgatcg caggcaagaa gctctaccgc gacctcgcag ggcgctaccg
      961 agagggtctg cgccgcatcg ggtgacctgc cttcatgtgt ggggagcgaa tcgctggcga
     1021 ccagcgttcg atagacacaa tacaccgagg aggaacccat gaaccacccc gaggcgaccg
     1081 ccatggccct gtccatcctg cgttccggca acccgttcca gtggtacgtg ataacccttc
     1141 tcgccctgac cctgtatgtc tacgtcaacg aatactccaa gaagaattac agggggatag
     1201 cggcaggcct gtccctgtac atggtccact ggttcgtgga gataggaaac gccctgatac
     1261 agcacttctc cggccatgcc ctctggaccg tgcccacggg caccgccttc ctcatcctcg
     1321 tcggcgtggg catcgagatc agcctcatgt tcgccgtctc agggctcatc ctgagcaagc
     1381 ttttgcccga agaccccggg atgaaggtcc tggggatcaa caacaggctg ttgttcgccg
     1441 tcgcgaacgc cgccttctac tcggtattcg agatcttcct ggtcaagacc ccgaccttcg
     1501 tgtgggtctg gccgttctgg ggggctttcc cggtgttcgt cacggtctac ataccgttct
     1561 tcctcgccgc gttctacgcg tacgactgga aaccgaggac gcagaagctg ttcatcggga
     1621 cgttgttcgc gataaacgca gcatcgctcg tggtcttcgc ggggatcctg aagtggatct
     1681 aggaaacggg ggaatcaccc ggctccggta atcggcctga cgaacgaggc ctcccccgtc
     1741 atcgacgagc gtttgaaggc cgtcacggcg gcacaaaggc gctctcttgc cccgtggatg
     1801 tcctgtgcca gtaaagacca gcgaagaccg agacagggct ccgtgcgaaa agaaacccag
     1861 gcctttgccg gtgcgggcca tcatgagcag ggatgccacc gggggtcgtc cccggcgaag
     1921 ccttccccgc gcccgggccg gcaggcgtca tgctgcgctt ggcaggccgc ctgcgcccgg
     1981 agagaagggc caaggcgtca atctttcagc cgctcgagct cggtcatgat ccattcgaga
     2041 ggcggcctct cgttgatgtc gtgaacgtcg agggcgcgga tgacgccagt cttgtccacg
     2101 atgaagatcg ccctctccgc cgtcccgtcc gacctgagca ccccgtagac atcggctgtc
     2161 ttgccgtgtg gccagaagtc agagagcacg gggaaccaca ggccgcccat gctcttggtc
     2221 cacgcgtaca gggtggggac gttgtccacg gagatcccga tcacgaccgc atccatggcc
     2281 tcgaactcct cgcgggccag gttgtacccc ggccactggt cagagcacac gggggtccag
     2341 gcggccggca cgaaggatat catcacgttc ttcttgcccc gatagtctct gagcgaaacg
     2401 gtcttcccgt ccaccgacct gaggctgaaa tccggcgcca cgtccccgac cttgaccttg
     2461 aggacgctgt ctatgggctt gagcttcccg acctggtaga tctcttcccg ggagagaccc
     2521 gcggaggaca aaggcgacgg cgcaccggcc ccggccatga gcatgcatac gatcgacaag
     2581 ggaaacacgt acctcttcat cttcatcgag acgcacctcg ctgtcacttc ggcagtgcca
     2641 tgagcaccct gtccaggaac cccctgggat cgtcgtgggg cccctgttcc acgaaaaacg
     2701 ccttgaacct tcccccggtc ttcttgaacg cgaagaacgt cggggtcctt ataccctcga
     2761 gcctgccgag gagaacggac ccggtgtcat cgaagagggg gaaggggatc ttgtacttct
     2821 tcttgaagaa cccgacctcg taggctgaat tccccacgcc tatgccgatg agtttcaccc
     2881 tgcccttgag tttgccgtcg gcctcgatga tcgcgaagag ctcgttggcc ttcggggcgt
     2941 ggcgctggca gtgcggacag tacatgctga agatctccac gatcaagata tcgcctgcga
     3001 tatccgagag agcgaacctc ccggctgtcg ccttgatgcc cagatacaac cgctcggcgg
     3061 cgtcggccgg gaccgcgagc gtcacggtct ctacggcggt gccgtcgggg gtggcgccga
     3121 aacacggctg caccagggcg aagagcagag agaacgcgag cgccgctatc ttgcacatgt
     3181 gctccccctt cgatgaacgc ccatccccga atgacggtga gtcatcgggg ataggcggaa
     3241 agaagaccat caccagttct cgccaaggat ctcgaagtag tcgcgcgggt gtgcacacgc
     3301 agggcacgat gccggagcct ctgtcccctc gtggaggtac ccgcagtttc tgcatctcca
     3361 gaccaccgcc ttgtcccgct tgaagaccct tccctgggcg atgttctcgg caagctcacg
     3421 gtagcgcttc tcgtgctgcc gctccgccac cgagaccgct tcccacacct tcgcgatggc
     3481 ctcgaaaccc tcttccctgg ccacccttgc gaacccgggg tacatctctg cctgctcgtg
     3541 gtgctccccc gcagcagccg ccttgaggtt ctcgagcgtg gagcctatca cgcccgcggg
     3601 gaagctcgcg gtgatctcga cctcccctcc ctcgaggaac ttgaagaacc tcttggcgtg
     3661 ctccttctcc tggttcgccg tctcttcgaa gatgtccgct atctgcatgt acccgtcctt
     3721 gcgggcctgg ctcgcaaagt aggtgtaccg catgcgcgcc tggctctccc ctgcgaacga
     3781 ggcaaggaga ttcttttccg tcttcgaacc cttcaacgtc gccatatcat tcctcctttc
     3841 atttccgtgt cttttccgga cccgcgagat cgttctttcg cgacgaccgt tcacgggatg
     3901 ccgcagcaga atccttcctg cactccgggc agagacccac gaagtcgatc tcgcagcttt
     3961 ggacctcata tcccgtggtt ctctccacgc cgtcggccct gtgcaacggc acgtcgtcca
     4021 cccggccgca cgaggagcac ctgatgtggg agtggtcttc gacgaaggcg tcgtaccgct
     4081 tcttgcccgt tccgaggtcg atcctcctga ccaggccttc cctggagagg agatcgaggt
     4141 ttcggtacac ggtgcccagg cttatacggg gcatgcagcg tcttaccgcc tcgtaaacgt
     4201 ctgccgccgt ggggtgggtc ctgacctttc tgagctcttc gaggatcaaa gcccgctgtt
     4261 tcgtcatccg gcttcttccc gcggccatcc gacacccctt gttttttgga atgattccta
     4321 ttaccatatt ggcgatggtt gtcaagggtt tccttcgcga ggcgacggaa cggatcgcgt
     4381 tgccaaacca aaaaggcggt gcggccatcg gcgaaggacg gcgcacgaaa agacctccac
     4441 gccgcgcccc cgaggcatcg gacacacccg ggctcacact tggccggccg cgcaccacgg
     4501 ttcggcacaa gggacaggtc tgcaggcaaa gggctatgcg cttcggtgtg gttgcccttt
     4561 ctcggctcgt atgctagaag gtgctacggt ccatgccctt acatggagaa agccaccttg
     4621 ggcaacgggc gctccccatg cttcgggagg gatcgtccgg cacagcttgg ccgttttccg
     4681 cccggccggg tctcccgaag ccgcttggcc ggagaggttc ccatgcttga aaacctgagc
     4741 gaggccctcg acctcgcaac gaaaacctcg accctgacgc cgatcgttct ggcgtaccta
     4801 gccggggcgc tcgtgagctt cacgccctgc gtatacccgc tcatccccgt caccgtgtcc
     4861 tacatcggcg cccaggaatc cgccgggcgt gcaacgggct tcatcctctc cctggcctac
     4921 gttctcggaa catccgtcgt ctataccgcg atcgggcttg ccgcggcctt ctcgggctcc
     4981 ttcttcgggt cgttccagac gaacccgtgg gtcaccttca cggtggcgaa cgtctttctc
     5041 ctcatgggat gcgccatgct ggagttcttc gtcttgccca tcccctcctt cctgagggcc
     5101 cagctctttt ccccgaaacg taggggcatc gctggggccc ttctcctcgg cctcgcatca
     5161 ggactcgtca tgagcccatg ctccgccccg gtcctcaccg tgctcctcgg atacgtcgcc
     5221 tcccgccaga acacgctcct gggctccctg atgctgtttt ccttctccct cggaatgggc
     5281 accctgctca tcatcctggg cacgttcacg ggcctcttga ggagcatccc cagatcgggg
     5341 ccgtggttgg tctacgtgca gaaaggcttc ggcctcgtgt tcgtcgccat ggccgaatac
     5401 tatctcgtca agacaggcat ctttttgatg tagactggag gctggcacat gaaaaagacc
     5461 cccctcgtcg ccacgtcggg cggcctcgcc gtcctcttcg cgggcctgct gatggtgctt
     5521 tacgcgcatc catcgtgcgc cgcagagccc cccgcatggc cggcccctca aagcaaggcc
     5581 gcctatgcac cgatcttcgt gcttcgggac atcgagaaca gggcggtttc cctgagggac
     5641 ttcaggggaa aggtggtgct cgtgtacttc accacgacct ggtgtcccta ctgcaagagg
     5701 gacatcccga ggctgaaaaa cctctatgcc tccatgaggg gcaagccttt cgagatcctg
     5761 gccgtgtacg tcaacgagag cacgtccagg gtgcgcgcgt tcgcggaaaa gtacgagctc
     5821 ccctaccggg tgctcgtcga taccgatgcc cacgtggcgc gtctttacgg cgtccggggt
     5881 gtgcccatgc gcgtggtggt cgacaaggcc gggaaggtgg gctgctacca gtgcccgtct
     5941 ccggaagacg ctatcggcga gctgttgagg cgctgacgag gaaggaccgg cgaggagccg
     6001 gccccggagg gccttcaccg tcatccaaag gcgttcggat ggggcacggc ctttctcgtg
     6061 ggacgggaag agccggtttt cgggtacgtg gcgaagtcgc gccggtcggc cgatggatcc
     6121 ttgcctgtgc cacgaggatg agctcctcca caaacagtat agctcacatc ctgtgtaccg
     6181 cttctggggc ctcccgtttg acgtctgctt gacgacgagc gtcaagctcg tcttcggcac
     6241 agaaaagacg ccccgagggt gctttctccg cctggaatgc cgcttcccgc cccagggcgt
     6301 tcagggtcgc cccgctcatg ccggtaccgt aaggccccct cgtcaccggg atccaccatg
     6361 gacgccttcc gtccccttga cgagcgaggc catgatcgat tcagccaccc tcacggcctc
     6421 gaccccgtcc ttcgcgtaag cggcgccgat ggcccgggcg aagtcaccgg tgacgacggc
     6481 gccgcccacc atgaccgcgc accgcagccc ctggtttctc aggtatctca ccgtgtcctc
     6541 catgcccgcc atggtggtgg tcatgagggc cgagagcccc acgatgtggg cgcccttctc
     6601 acgggccgct tcagctatcc tttccgccgg gacgtctttg ccgaggtcct ccacggtgaa
     6661 cccgttgttc ctgagcatga ggcccacgat gttcttgccg atgtcgtgaa tgtcgcccct
     6721 caccgtcgcg agaaccacga cccccttcgt cgccgcggat gcaccccccc tgtcgagaag
     6781 gggttcgatc cgctcgacgg cgagcttcat ggcctcggcg ctcgcgatca gctgggggag
     6841 gaagtagacc ttgctctcgt agagacggcc gacctctcgg atagccggta tcatgacctc
     6901 gtccacgagc ctgcccggct cttccccttt gtccagcgcc atgcctacga gctccacgat
     6961 ccgttcgcgg tcaccctcga ggatcgcccg ggcgaccagg gatgcagggc tctcgtgcgt
     7021 ccttggctgg ggcttttcgg ggaccaccgc cccgaacttg gcgatgtagg cgtgtgcgtc
     7081 ccggtccgag cccctgagca cgtctacggc gtgcttgacg ctcatgacct cctcgctctc
     7141 cgggttggcg atcgcgcagg aaaggccatt cgccgaggcc atggccagaa acgccgcgtt
     7201 cacc
//
LOCUS       DAOSCR010000050         7182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6688_length_7182_cov_3.423039, whole genome shotgun sequence.
ACCESSION   DAOSCR010000050 DAOSCR010000000
VERSION     DAOSCR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6688_length_7182_cov_3.423039"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..933
                     /locus_tag="PLT69_02335"
     CDS             169..933
                     /locus_tag="PLT69_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOJ13318.1"
                     /translation="MPRVFVGTSGWNYDHWRGPFYPDGLPKNRWLGHYMRFFASVEVN
                     STFYRTMRPSTFEKWGRSSPVGFVWSLKASRFITHIRRLRDTGEPLRRFEESVSLLGE
                     KLGPILFQLPPSLAFDRESAAAFLRLRPSHELWAIEGRHPSWARDEALACLEEAGVSW
                     CISDTAGRYPLREAVTAPFVYLRLHGSKRLYASCYTKDELETWAGKILSYGRDAYVYF
                     DNDFMGYAPKNALELMHILEAYGTLCIPGGPGYYEG"
     gene            958..1488
                     /locus_tag="PLT69_02340"
     CDS             958..1488
                     /locus_tag="PLT69_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOJ13319.1"
                     /translation="MGRHLSTTAWAIVCSILALTGTPWAFELTSPAFTAGTEIPSVYT
                     CQGRDISPPLSWSGAPAGTKSLVLVCEDPDAPLGTWVHWVYYDIPPSLSRLPEGIPKT
                     ARPPQGGTQGKSSFGDLGYGGPCPPWGTHRYFFRLYALDTVLGIPEGAKKKQVLEAMK
                     GHVLATAELMGTYKKK"
     gene            1595..2524
                     /locus_tag="PLT69_02345"
     CDS             1595..2524
                     /locus_tag="PLT69_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13320.1"
                     /translation="MVRPSYRELLHTGELQRRVERALGLLKDCRICPRSCGVDRLGSE
                     KGFCRTGRYARVASFGPHFGEEAPLVGTHGSGTIFMSSCNLMCTFCQNHEISHANEGA
                     EVDALELASIMLDLTERGCHNINFVTPTHVVPQILEAVLVAARQGLDVPLVYNTGGYD
                     DASTLRLLDGVFDIYMPDFKFWDEGPAAAFCRAPDYREKACEAIREMHSQVGDLEIDE
                     RGLAVRGLLLRHLVMPEGLAGTSRVMEFIATQISRDTYVNVMDQYHPCYRARTDRLIG
                     RRITASEYMEALEAAMRAGIHRLDPHSRFAFIT"
     gene            3054..3992
                     /locus_tag="PLT69_02350"
     CDS             3054..3992
                     /locus_tag="PLT69_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOJ13321.1"
                     /translation="MGVFAVTFTAVAMLLTIGILGYWMLSRRVIAGEALKPLSVLAID
                     IALPCLTFTSILKGFDPADNASWWTLPLWWLGSTAWFTALALVSSRLASPWCRREFTV
                     ALLYQNAIFFPLAIIVELEGTGSALLVDLFLYTLFFPAFLFNTAPMFFAGTGQKSPYR
                     ILNPVLAVTLAGIAIRLFGVSGFVPAFLADGLSRVGAMAVPLLMLVLGGNIYLDMLGA
                     RKPAYRDVTAFVCMKNLVFPAATLGMLLLVRPPHAPAFIIMLQASVPPVTAVPIIVER
                     EGGDRNVTNQFMVASFLFSLVSIPAMIALFTSFFPG"
     gene            4310..5814
                     /locus_tag="PLT69_02355"
                     /pseudo
     CDS             4310..5814
                     /locus_tag="PLT69_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(6017..6958)
                     /locus_tag="PLT69_02360"
     CDS             complement(6017..6958)
                     /locus_tag="PLT69_02360"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ13322.1"
                     /translation="MNESICILAGNANKPLAQAICTIIGCPLGRGDVKRFSDGETSVD
                     IHESIRGRDIFIVQPTCPPTNEHLMELLVMLDATRRASAKRITAVIPYFGYARQDRKA
                     SPRAPISAKLVANLLTVSGADRVLTMDLHAGQIQGFFDIPVDNLYAKPVILDYVRNRY
                     DGDLCIVSPDAGGVERARAYAKPLKADLAIIDKRRERANESNVMHIIGSVTGKTCLIV
                     DDIVDTAGTLTNGAAALIGEGAKRVVASCTHGVLSGPAISNLAGSAIEELIVTDTIPL
                     REEAHSLGKITVLTVAPLLAEAIKRIHTGSSVSELFL"
     gene            complement(7039..7112)
                     /locus_tag="PLT69_02365"
     tRNA            complement(7039..7112)
                     /locus_tag="PLT69_02365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7078..7080),aa:Gln,seq:ttg)
BASE COUNT         1195 a         2426 c         2309 g         1252 t
ORIGIN      
        1 acctgaagac gagggatgtc gacgccaccg cacgggaggt gacggacctc ttcatgtccc
       61 acagcagcgg ttacttcctg ccccgcgagg tgatggagat ggtcgtggga cagatgacca
      121 ggttcctcgc gcgttcgctc gagcccggct cggcctgagg gggcggccgt gcctcgggtg
      181 ttcgtgggga cctcgggctg gaattacgac cactggcggg gccctttcta tcccgatggc
      241 ctccccaaga acaggtggct cggtcactac atgcgcttct tcgcttccgt ggaggtgaac
      301 tccaccttct accgcacgat gaggccgtcc accttcgaga aatggggccg ttcttcgccg
      361 gtcggcttcg tgtggtccct gaaggcgagc aggttcatca cccacatcag gcgcctgagg
      421 gataccggtg aacccctcag gagattcgag gaatcggtct ctctcctggg agagaagctg
      481 gggccgatcc tcttccagct cccgccgtcg ctggccttcg accgggaatc ggccgcggcc
      541 ttcctgaggc tcaggccgag ccacgagctc tgggcgatcg aggggaggca cccgagctgg
      601 gcccgcgacg aggcgctcgc ctgcctcgag gaggcgggcg tgtcctggtg catctctgac
      661 acggccggga ggtacccgtt gcgcgaggcc gtgaccgcgc ccttcgtcta cctcaggctt
      721 cacggctcga agcgcctcta tgcctcctgc tacacgaaag acgagctcga gacatgggcc
      781 gggaagatcc tctcgtacgg cagggacgcc tacgtgtact tcgacaacga cttcatgggt
      841 tatgccccga aaaacgcgct cgagctcatg cacatcctgg aggcatacgg caccctttgc
      901 ataccggggg ggcccggtta ttatgaaggg tgagcacacg aaagaggagg gtcacccatg
      961 ggacgacacc tttccacgac cgcttgggcc atcgtatgct cgatcctcgc cttgacggga
     1021 acgccctggg cattcgagct cacaagccct gccttcaccg ccggcacgga gataccgtcg
     1081 gtctacacct gccagggcag ggacatatcg ccgccgctct cgtggtccgg ggcccctgcg
     1141 ggcaccaaga gcctcgtcct cgtctgcgag gaccccgacg caccgctcgg cacctgggtc
     1201 cactgggtgt actacgacat cccgccctcc ctgagccgcc tccccgaggg gatacccaag
     1261 accgcaaggc ctccccaggg ggggacccag ggaaagagca gcttcggcga tctcggctac
     1321 ggcgggccct gcccgccctg gggtacgcac cggtacttct tcaggttgta cgcgctcgac
     1381 accgtgctgg ggatccccga gggcgccaag aagaagcagg tcctcgaggc gatgaagggg
     1441 cacgtccttg ccacggctga actcatgggc acgtacaaga agaagtgagc cctcctcgag
     1501 cctttgggcc gcacccggag cgtcgagaag gaacaggaag agttagggac gttcaagaga
     1561 ccgtgagttc atcacgtccg ggccgggcac acccatggtg agaccttctt acagggaact
     1621 gctgcatacg ggggaactcc aaaggcgcgt tgagcgcgca ctcggcctcc tgaaggattg
     1681 caggatctgc ccaaggtcgt gcggggtgga caggctcggg agcgagaagg gtttctgccg
     1741 gacaggcagg tatgcccgcg tcgcgagctt cggcccgcac ttcggtgaag aggcgccgct
     1801 ggtgggcacc catgggtcgg gcacgatctt catgagctcg tgcaacctca tgtgcacctt
     1861 ctgccagaac cacgagataa gccatgcgaa cgagggcgcc gaggtggacg ccttggagct
     1921 cgcatccatc atgctcgacc tcaccgagcg gggctgtcac aacatcaact tcgtgacccc
     1981 cacgcacgtg gtcccccaga tcctcgaggc cgtgctcgtg gcggcccgcc aaggtctcga
     2041 cgtgcccctg gtctacaaca cgggcgggta cgacgacgca tccaccctgc gcctgctgga
     2101 cggcgtgttc gacatctaca tgccagactt caagttctgg gacgaaggcc ctgccgccgc
     2161 cttctgcagg gcgcccgact accgggaaaa ggcgtgcgag gcgatacggg agatgcactc
     2221 ccaggtggga gacctggaga tcgacgagag gggactcgct gtcaggggcc tccttctgcg
     2281 ccacctcgtc atgcccgagg gcctcgcggg gacaagccgc gtgatggagt tcatcgcgac
     2341 gcagatctcg cgggacacgt acgtgaacgt gatggaccag taccaccctt gctacagggc
     2401 gcgcacggac cgcctgatag gaaggaggat caccgcgtcg gagtacatgg aggcgctgga
     2461 agcggcaatg agggcgggca tccacaggct ggacccgcac tcccgcttcg ccttcatcac
     2521 gtgagggacg cgggacgccg gcgcacgcct ccggtctccc gtccgcagta caggggcggc
     2581 ccgcagtgga gtgcgtctcg agatcggcat atccccatcc gtcctcaagt aacaggggca
     2641 atccacccct acgcctcgtg caaggggatg ctgcatcggg tcgctatacc aacgattggc
     2701 gccttgtccc gcctgcagcc gtccgtggtg gaccgccggc cccgcactcc cgtgtgccgc
     2761 cgaacccggg cccaggcgag acgacagacc accacgcagg tgtaccccat cctctccgca
     2821 tgtgccgcag acagggcgac ccttaagggt cagccccgta gagggggccg ttacgtcttg
     2881 ccggtcctca accaggggcc tggagtgcgg ctcagaaaag cctgcctcca gcggacacgc
     2941 ccgggcggcc atgggacctg acgacgtgca gcctggatcc agctggcgga gggtgcgtgg
     3001 acctgcggac aaaaccctcg attcccatcg aagactcgtg tagatgtgca gccatgggcg
     3061 tgttcgcagt cacgttcacg gcagtggcca tgctcctgac catcggcatc ctgggatact
     3121 ggatgctgag caggcgcgtc atcgcgggcg aagccttgaa gccgctctcc gtgctcgcca
     3181 tagacatagc cctgccgtgc ctgaccttca cgtccatcct gaagggcttc gaccccgcag
     3241 acaacgcctc ctggtggacc ctccccctgt ggtggctggg ctccacggct tggttcaccg
     3301 cgctcgccct cgtctcctcc cggcttgcaa gcccctggtg caggcgcgag ttcacggtgg
     3361 ccctcttgta ccagaacgcg atcttcttcc cgctcgcgat catcgtcgag ctcgaaggaa
     3421 cggggtccgc cctgctcgtg gacctcttcc tgtacaccct cttcttcccc gccttcctgt
     3481 tcaacaccgc accgatgttc tttgccggga ccggccagaa aagcccctac aggatcctca
     3541 acccggtgct cgccgtgacc ttggccggca tcgcgatccg cctcttcggc gtctccgggt
     3601 tcgtgccggc cttcctcgcg gacgggcttt cgcgggtggg cgccatggcc gtccccctcc
     3661 tcatgctggt cctgggcggc aacatctacc tcgacatgct gggcgccagg aagcccgcct
     3721 acagggatgt gaccgcgttc gtctgcatga agaacctcgt cttcccagcg gcgacgctgg
     3781 gcatgctcct tcttgtaagg ccccctcatg cgcccgcctt catcatcatg ctccaggcgt
     3841 cggtccctcc ggtcaccgcc gtccccatca tcgtggaacg cgaaggcggc gacaggaacg
     3901 tcacaaacca gttcatggtg gcgagcttcc tcttctcgct cgtgagcata cccgccatga
     3961 tcgcgctctt cacctccttc ttccccggct gacagccact ctcggcccga gccccgcgac
     4021 aggcgccctc gaccctcctt aagcctcatg ccgcaaaggt tgttcccctc attcttcagc
     4081 cgagcggccc ttccatcgaa ggggttgtgc gggtccgcag gggttcgcca cctacggaga
     4141 gaggcctgca cgatcccgag ggcttcaccg ccctcacacc cgggcctgaa ggcccgcacc
     4201 cgggagcact catccctgtc gggccgcgca ggaacgcaca accgacacaa cctcccgcag
     4261 ccgcagcctg cttgtgcttc tccagggtcg tctggtatca tcgaggcgga tgatagcgca
     4321 cgtgcactcg gccgccccct ggggggctga tgccctggcc gtcgtggttg aggttgatat
     4381 tcaaggaggt ctgccgagct tcaccgtcgt cggactgccg gactccgtcg tgaaggagag
     4441 cagggagagg gtgaggtcgg ccgtcgtcaa ctccggcctc gacttcccgc ccaggcgcat
     4501 caccgtgaac ctcgcacccg cccacatccg gaaggagggc ggggcgttcg acctgccggt
     4561 ggcggtcgcg atcctggcgg cgctcggcgc agtccccagg gggcgcgccg agagtcacct
     4621 cttcgtcggc gaactcggcc tggacgggct catcaggccc gtgagggggg tcatatcggc
     4681 ggccttcgtc gcctcgagga tgggcatgca gggcatcgtc tgcccaaggg acaccgcacg
     4741 cgaggcgggc ctcgcaggca tcggcgtcta cccggcggac accctgctcg acgtgtacgc
     4801 attcctcaag ggcgagcgcg agctctccca cgggacaggc ccccaggtca cggccgggct
     4861 gtcgaggccg gacgccctgc ccgacctcgc cgaggtgtgc ggacaggtca tgggcaagag
     4921 ggggctcgag atcgccgcgg cggggggcac aacctcctct tcgtgggccc ccccggggcg
     4981 ggcaagtcga tgctcgccca gaggcttccc tcgatcctgc ccgggctcac gcccggggag
     5041 atcctcgagt gcaccgccgt ctactccgca gcagggctcc tcggccggga ctcggtcgtc
     5101 acccaacgcc ccttcagggc gccccaccac acgatctcgg acgccgggct catcggtgga
     5161 ggcagcatgc ccaccccggg cgaggtgagc ctcgcccaca ggggggtcct cttcctggac
     5221 gagatgccgg aattcaggcg gtctgctctc gaggcgctgc gccagccgct cgaagactgc
     5281 acggtcagga tctcgagggc ggctgcatcc ctgtccttcc cggcggactt ccaactcatc
     5341 gccgccatga acccttgccc ctgcggcttt ctgggtcacc cggacagacc gtgctcctgc
     5401 acccccgtcc aggtagccag gtaccgggcg cgcatatccg ggccgctgct cgacaggatc
     5461 gacctgcacc tctggataga ccccgtggat gcctccggga tcttcaaccg ggggcaaggc
     5521 gcgtcttccc acgaggtgcg cgccagggtg gaggccgcac gagagatcca gctcgccagg
     5581 gggttcatca acgcccgcat cccccaggaa tcaatggagc gcatctgcag gctggacacg
     5641 gcgtccaggg gcatgctcgt caatgccgtc aagcgcgcgg cgctgagcat gcgggcactg
     5701 ggcagggtca tcaaggtggc ccgcacgatc gccgacttgg aggggagccc ctccatcagg
     5761 gacgtgcacg tggccgaggc gctccagttc aggccgggga tggtcgagga ctaggccggc
     5821 ccggcgcgcc cgcagggacg cctcccgccg gacgccctgg aagaatgagt atgcctgcac
     5881 accgagaaac tcccctcttg ccggggacgc ccgagggttg aggtgggcgt atcaggcccc
     5941 cctcatgacc acgccgagaa actcccctcc tgcctggccg actgagtgac ccctctcgtt
     6001 cggggcaaac cttgtctcag aggaacagct cgctcaccga cgacccggtg tggatgcgct
     6061 tgatggcctc ggccaagagc ggcgccacgg tgagcaccgt tatcttgccc agggagtgcg
     6121 cctcttccct cagggggatg gtatccgtga cgatgagttc ttctatggcc gacccggcaa
     6181 ggttggagat ggccgggccc gagagcacgc cgtgggtgca gctggccacc acgcgcttcg
     6241 ctccctcgcc gatgagtgcg gccgccccgt tggtcagggt cccggccgtg tcgacgatat
     6301 cgtcgacgat gaggcacgtc ttgcctgtga cggagccgat gatgtgcatc acgttggatt
     6361 cgttggcacg ctcgcgccgc ttgtcgatga tggcgaggtc ggccttcagg ggtttcgcgt
     6421 aggccctggc cctctccaca ccccccgcat cgggcgagac gatgcacaag tcgccgtcgt
     6481 agcggtttcg gacgtagtcg aggatcacgg gcttggcgta gaggttgtcc acggggatat
     6541 cgaagaagcc ctggatctgt cctgcgtgca ggtccatggt gaggacgcgg tcggcacccg
     6601 agacggtcag caggttggcc acgagcttgg ccgagatggg ggcgcggggc gaggccttgc
     6661 ggtcctgcct cgcgtagccg aaatagggga tcaccgccgt gatcctcttg gctgacgcgc
     6721 gcctggtcgc gtccagcatg accaggagtt ccatgaggtg ctcgttggtg ggcgggcacg
     6781 tgggctgaac gatgaatatg tcccttccgc ggatgctctc gtggatgtcg acggacgtct
     6841 cgccgtccga gaacctcttg acgtcgcccc tgccgagggg gcacccgatg atcgtgcaga
     6901 tcgcctgagc caagggcttg tttgcattgc ccgcaaggat gcagatactc tcgttcatat
     6961 cgccttccag gtgaagaggt gtgtacccgg gacagcggcc cttgagtccg ccttcttgtt
     7021 ttcaagctac tgcgggcatg gctgggacgg gaggattcga acctccataa cgagatccaa
     7081 aatctcgtgt cctgccgtta gacgacatcc cagcaccaga gggggccatc cggttaccgg
     7141 gagcgcacta tagttgaatc agacctcgtt ggcaatcttt tg
//
LOCUS       DAOSCR010000051         7062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6836_length_7062_cov_2.591551, whole genome shotgun sequence.
ACCESSION   DAOSCR010000051 DAOSCR010000000
VERSION     DAOSCR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_7062_cov_2.591551"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLT69_02370"
     CDS             <1..569
                     /locus_tag="PLT69_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13323.1"
                     /translation="ILDMDIDERARFGIGMAFQYPPAVKGVRLGVLLKHVNSDGNWVQ
                     TSAEMLNMTSYVDRDLNVGFSGGERKRSEILQLMVQMPDLALLDEPESGVDIENISLI
                     AQGLRVMLEKDKPISRRQRSAIVITHTGLILDYIKADIAYVIMDGHVLGCGAPGEIFD
                     EIRQHGYKKCTMCFAEKYRDMNTQDLEK"
     gene            569..1651
                     /locus_tag="PLT69_02375"
     CDS             569..1651
                     /locus_tag="PLT69_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOJ13324.1"
                     /translation="MDIAPERLVSMGYDSCAASCGRFMVLDRDILEFSCGISGLHVMP
                     LKTAFEVYPHVRENLLFGLIPKDKNDFTKMASRVDPVGYYVRVEKGVKIADPMQAAFL
                     FGGAHQDQLIHNIIELEEGASLDIVNGCANTHAGTSGRHIGITETYLGKGSSLGYTMI
                     HNWGPDMEVYPVGAVQVGEGARYLSNYVSTSPVKRIETYPKATVGPHASARYYSIIYA
                     RQSSYFDTGAFVALEGEGSSGEIISRVVSEGGTVISRQTISGETKGCIGHMECSGLLL
                     AENGVIHSIPELRGKHPDIDLSHEASIGRISDEEVSYLMTRGLGKEEARSLIIRGFLD
                     VKIAGLPDRVKVIVDEVIEQAMSGKI"
     gene            complement(2309..3457)
                     /gene="argJ"
                     /locus_tag="PLT69_02380"
     CDS             complement(2309..3457)
                     /gene="argJ"
                     /locus_tag="PLT69_02380"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOJ13325.1"
                     /translation="MTPRGFRFSAVNAGVKAPDVKSLDLGLIYAESPFVLSGMFTRNI
                     VKAAPVIIAMEQAKRGSARAIIANSGNANACTGATGLADARSVMQAVAKELDIRPDEV
                     VPMSTGTIGVYLPTERIVHKVPELVQKLSDDPEPFARCIMTTDTTLKVSRRRVGQACI
                     LGIAKGAGMIAPDMATTLAIVLTDAVMDKDTLDKVLKGVVAHTFNAISIDGDMSTNDT
                     ILACASSQVDADPEEFAYAFDEVVTELAHMIVKDGEGATKFVTVKVMGCANDRDARLI
                     ARAVADSLLVKTALFGADPNWGRIVAAVGYAGVNIDPDRISVRIQGLDVVTSGVQDRS
                     FREEDLKEALQQHDITIEITVGGGMGRFTVWTTDLSHKYIEINASYRT"
     gene            complement(3454..6084)
                     /gene="secA"
                     /locus_tag="PLT69_02385"
     CDS             complement(3454..6084)
                     /gene="secA"
                     /locus_tag="PLT69_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOJ13326.1"
                     /translation="MINWLIKLFGSKNDRELKRMRPIVEAINALEDTMRAKSDAELKA
                     QTPRFKEMVDAGASLDDILPEAFATVREVSRRTLGMRPFDVQLIGGIVLHEGKIAEMK
                     TGEGKTLVATMPVYLNALLGRGVHVVTVNDYLARRDAEWMGAIYRFLGMDVGVIVHGL
                     DDPQRRKSYHADITYGTNNEFGFDYLRDNMKFSLEDYVQRDFYYAIVDEVDSILIDEA
                     RTPLIISGPAEESTSKYYEANAAVLDMLRRKDAETLYVKDEKANTVMLTEEGIEFLQR
                     RLGKANLYDPAEFETVHHLNQALRAHLLFSKDVHYVVKDGEVVIVDEFTGRLMPGRRY
                     SEGLHQALEAKEGVKVANENQTLASITFQNYFRMYEKLAGMTGTAETEALEFKNIYGL
                     DVIVIPTNLWMIRVDHPDVIYKTEKEKYRAVIDQILACYEKGQPVLVGTSSIENSEKV
                     SNLLKKRGVPHNVLNAKHHEREAEIVAQAGRKKAVTIATNMAGRGTDIKLGGNPEFLA
                     KQRVLPDDPTYPEILAHFTEQCAREHEEVVAAGGLFILGTERHESRRIDNQLRGRSGR
                     QGDPGESRFYLSLEDNLMRIFGSERIASIMDRLGVAEDEPIEHRLVSKSIEGAQKKVE
                     GRNFEIRKHLLEFDDVMNKQREVVYTQRKRILSEENLKPLVEEMVEETVRDAIETYLP
                     RKGSKEDRDLEGLVTWVKNIFDVSVEIPRDKVTDHEAVFDIIKSALMDTYEKREQEFT
                     PEVMRMVERHFLLLTLDTLWKDHLLSMDHLKDGIGLRGYGQKNPLREYQREGFDMFLN
                     TLDRVRELSLERLFKVQIQRQEDVEKVSRQERRQRIQMGRSAQERPSTVRRTGKKVGR
                     NDPCPCGSGKKYKQCCGVDA"
     gene            complement(6261..>7062)
                     /locus_tag="PLT69_02390"
     CDS             complement(6261..>7062)
                     /locus_tag="PLT69_02390"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOJ13327.1"
                     /translation="VRRGEDVQSGYDFDFSHFVSGFDVEAVGRRAVERAAALLGARRV
                     ATRRMPVLFDSSSASEIFDFISESFLGENVLKGKSFLKDSLGMDRFSACLTIVDDPLD
                     VHAADCCPFDGEGMPSMRTVLVDRGTVSAFLYDAYWARRSGLSSTGNSVRSSYRAWPT
                     LASRHISVVPGTVPLREVLSGIPAVLKVMDIMGMHTANPITGEMSVGINGILLEHGEP
                     VHPVREAAIAGNIFDMLGRVIAVGDDARAFGHVLCPSILVDSVDVGAQ"
BASE COUNT         1306 a         2123 c         2107 g         1526 t
ORIGIN      
        1 gcatcctgga catggatatc gacgagaggg cgcgtttcgg tatagggatg gccttccaat
       61 acccgccggc tgtcaagggg gtacggctcg gggtgctcct caagcacgtg aatagcgatg
      121 gaaactgggt gcaaacaagc gcagagatgc tcaacatgac gtcatatgtg gaccgggacc
      181 tcaacgtggg attctccggg ggcgagcgga agcgatcgga gatcctccag ctcatggtcc
      241 agatgcccga cctcgccctc ctggacgagc ccgagtcggg agtggacata gagaacatat
      301 cgctcatcgc gcagggcctg cgtgtcatgc tcgagaagga caagcccata tccaggcgac
      361 agaggtccgc gatcgtgatc acgcacacag ggctcatcct tgattacatt aaggctgaca
      421 tcgcctacgt gatcatggac gggcacgtgc tggggtgcgg ggcgccgggc gagatattcg
      481 acgagatcag gcagcatgga tacaagaagt gcacgatgtg cttcgcagag aaataccgtg
      541 acatgaacac ccaagacctg gagaagtgat ggacatcgca cctgaacgac tggtctccat
      601 ggggtatgac tcctgcgcgg cgagctgcgg ccgcttcatg gtgctggatc gggacatcct
      661 cgagttttcg tgtgggatca gcggccttca cgtgatgccc ctcaagacgg ctttcgaggt
      721 ctatccacac gtgagagaaa acctcctgtt cggcttgatc cccaaagaca agaacgattt
      781 cacgaagatg gcgtctcggg tggatccggt gggctactac gtgcgggtgg agaagggcgt
      841 caagatcgcc gaccccatgc aggcggcgtt tctcttcggt ggggctcacc aagaccaact
      901 catccacaac atcatagagc tcgaagaagg ggcctcgctc gacatcgtga acgggtgcgc
      961 gaacacccat gccgggacat cgggcaggca cataggcata acagaaacct atctgggaaa
     1021 aggttcatcc ctcgggtaca cgatgataca caactgggga cccgacatgg aggtctaccc
     1081 cgtgggggcc gtgcaggtag gggagggggc aaggtacctt tcaaactacg tctccacctc
     1141 gccagtgaag aggatcgaga cgtatcccaa ggccacggtt ggaccgcatg catctgctcg
     1201 atattactcc attatttacg ccaggcagtc ctcgtatttc gacaccggcg cctttgtggc
     1261 cctcgagggt gaaggttcgt ccggggagat cataagcagg gtggtttccg agggtggtac
     1321 cgtgatatcg aggcagacga tctccggaga gacaaaaggc tgcatagggc acatggagtg
     1381 cagcggtctc ctccttgccg agaacggcgt gatccactcc ataccggagc tcaggggcaa
     1441 gcaccctgat atcgacctgt cccacgaggc ctcgataggg aggatatcgg acgaggaagt
     1501 gagctacctc atgaccaggg ggctcggcaa ggaagaggcc cgttcgctta tcatcagggg
     1561 attcctcgac gtgaagatcg cgggcctgcc ggacagggtc aaagtgatcg tggacgaggt
     1621 catcgaacag gccatgtcgg gaaagatctg acccccgcga ggatcatcac gggttctggg
     1681 atcgccaggt tgcccacaaa caggccccgg aagctgtttc acaggttttt ctctttattt
     1741 gcgccaggat gcgtttcatg agcaaggtca atggtttccc atgggttgaa tgaacctggc
     1801 agggcgcgat aaactgtcta ggagttctcg acaccgatta tggtgtagat atctttttcg
     1861 catgatttcg aaatgggccg cttttctgaa tagaaagatg cgaagtaaaa ccatggtcga
     1921 tatgtctctg catgccttca tcgatgatgg tcgtcgtgtc gccaaggaga tctgggtgga
     1981 cacgatcggc acatgatcat cgcatctctc gcgtatcctt tgagatatac atacgaagcg
     2041 gtgcatacct catgcaaagg tccagcgtga tgagggaatc ctcggtgtgc aagacccttt
     2101 ccatggtcac catgtcgtcc ctttctgcct gctgggtccc cttagacgat ctgtgaagag
     2161 gaaggaagat gttcccggtc tctaatttaa cataacatat attatcggtc aaaaaggtat
     2221 ccacaggcag gcgctccttg tcgctccgtg taggcgtgct tcccgcggca ggggcgcccc
     2281 actttcgtga gggcgagctc aaccagcctc aggttcggta cgatgcgttg atctcgatat
     2341 acttgtggga gagatccgtg gtccacacgg taaaccgtcc catgccgccg cccacggtga
     2401 tctcgatggt gatgtcgtgc tgttgcaggg cttctttcaa gtcctcttcc ctgaaagacc
     2461 tgtcctgcac gcccgaggtc accacgtcga gaccctggat cctcaccgat atcctgtccg
     2521 gatctatgtt cactcccgcg tatcccactg cggcgacgat cctcccccag ttggggtccg
     2581 ccccgaacag ggcggtcttg accagcaggg aatcggcgac cgctcttgct ataagccttg
     2641 catccctgtc gttggcgcac cccatgacct tgacggtgac gaacttggtg gcgccctcgc
     2701 cgtccttgac gatcatgtgg gcaagctcgg ttaccacctc gtcgaatgcg taggcgaact
     2761 cttccgggtc tgcatccacc tgggacgacg cacacgcgag gatggtgtcg ttcgtggaca
     2821 tgtccccgtc tatgctgatg gcgttgaagg tgtgggccac cacccccttg agcaccttgt
     2881 cgagcgtgtc cttgtccatg acggcgtctg tgaggacgat cgcgagcgtg gtggccatgt
     2941 ccggggctat catcccggcc cctttcgcga tcccgaggat gcacgcctgg cccacccgcc
     3001 tcctcgacac cttcagggtg gtgtcagtgg tcatgatgca tcgcgcgaaa ggttcagggt
     3061 cgtcggatag tttctgtacg agctcaggca ccttgtgcac gatgcgctcg gtaggcaggt
     3121 atacccctat ggtccccgtc gacatgggaa cgacctcgtc gggtcttata tcgagctctt
     3181 tggcgaccgc ctgcatgacc gacctggcgt ccgcaaggcc tgtggcaccg gtacacgcgt
     3241 tcgcgttgcc cgagttggcg atgatcgcac gcgcactccc cctcttggcc tgttccatgg
     3301 cgatgatcac cggggccgct ttcacgatgt tcctcgtgaa catgccggac aggacaaacg
     3361 gtgactcggc gtagatgagg ccgaggtcaa ggctcttcac atcgggggct ttcaccccag
     3421 cgttgaccgc agagaagcgg aaccccctgg gggtcatgcg tcaaccccgc aacattgctt
     3481 gtattttttg cctgaaccac atgggcaggg atcgttcctg cccaccttct tgcccgtcct
     3541 cctgacggtg gacggccttt cctgagcgga cctccccatc tggatcctct gccttctttc
     3601 ctgcctcgat accttctcga cgtcctcctg tctctggatc tggaccttga acagccgctc
     3661 gagggagagt tccctgaccc tgtccagggt gttcaggaac atgtcgaagc cctcccgctg
     3721 gtactccctg agcgggtttt tctgcccgta tcccctcaac cctatgccgt ccttgaggtg
     3781 atccatcgac aagaggtgat ccttccacag ggtatccagg gtgagcagga ggaagtgacg
     3841 ttccaccatc cgcatgacct cgggggtgaa ctcctgttcc cgcttctcgt aggtgtccat
     3901 gagggcagac tttatgatat cgaagaccgc ctcatggtcg gtgaccttgt cgcgcggtat
     3961 ctcgacgctc acgtcgaaga tgttcttcac ccacgtcacg aggccctcta ggtccctgtc
     4021 ctccttggaa ccctttctcg ggagataggt ttcgattgcg tctcgaacgg tttcttccac
     4081 catctcctcg acgagcggct tgagattctc ctcggagagg atcctcttcc tctgcgtgta
     4141 caccacctcg cgctgcttgt tcatgacgtc gtcgaactcg aggaggtgct tccttatctc
     4201 gaagtttctt ccctcgacct tcttctgtgc gccctcgatg ctcttgctca cgagcctgtg
     4261 ttctatgggc tcgtcctcgg cgaccccgag tcggtccatg atcgaggcga tacgttccga
     4321 gccgaagatc ctcatgaggt tgtcctcgag gctcaagtag aagcgggact cgccagggtc
     4381 tccctgcctg ccggatcgcc ccctgagctg gttgtcgatg cgcctgctct cgtgacgctc
     4441 ggtgccgagg atgaaaagcc ctccggccgc taccacctct tcgtgttcgc gggcgcactg
     4501 ttcggtgaag tgagcgagga tctccggata ggtgggatcg tcgggaagga cgcgctgttt
     4561 cgccaggaac tcggggtttc cgccgagctt gatgtccgta cccctgccgg ccatgttggt
     4621 cgctatggtg accgccttct tcctccccgc ctgcgcgacg atctcggcct cgcgctcatg
     4681 gtgcttggca ttgagcacgt tgtggggtac cccacgtttc ttgagcaggt tcgagacctt
     4741 ctcggagttt tctatggagc tcgtacccac gagcacgggc tggcccttct cgtagcaggc
     4801 caggatctgg tcgatcactg cgcggtactt ctccttttcc gtcttgtata tgacgtcggg
     4861 gtgatccacg cggatcatcc agagattcgt cgggatcacg ataacgtcca gtccgtagat
     4921 gttcttgaac tcgagcgcct cggtctccgc agtgcctgtc atgccggcga gcttctcgta
     4981 catgcggaag taattctgga aggtgatgga ggcgagcgtc tggttctcgt tggccacctt
     5041 caccccctcc ttcgcctcca aggcctggtg aagaccctcg gagtaccgcc tgcccggcat
     5101 gagcctgccg gtgaactcgt ccacgatgac gacctcgccg tccttgacga cgtaatgcac
     5161 atccttggag aacaaaaggt gggccctcag ggcctggttg aggtggtgaa cggtctcgaa
     5221 ctcggccggg tcgtagaggt tcgccttgcc gagccttctc tgcaggaact cgatcccctc
     5281 ctcggtgagc atgaccgtgt tggccttttc gtccttgacg tagagcgtct cggcatcctt
     5341 tcggcggagc atgtccaaga ccgcggcgtt ggcttcgtag tacttcgagg tggactcttc
     5401 cgcggggccg gatatgatga ggggcgtgcg cgcctcgtcg atgagaatgg agtccacctc
     5461 gtcgacgatg gcatagtaga agtcccgctg gacgtagtcc tcgagggaga acttcatgtt
     5521 gtccctcagg tagtcgaaac cgaactcgtt gttggtgccg taggtgatgt ccgcgtggta
     5581 gctcttcctc cgctgggggt cgtccaggcc atgcacgatg acccccacgt ccatgccgag
     5641 gaacctgtag atggccccca tccactccgc gtccctgcgg gcgaggtagt cattgaccgt
     5701 caccacgtgg acaccccggc ccagcagggc gttcagatat accggcatgg tggcgacgag
     5761 cgtcttgccc tcgcctgtct tcatctcggc gatcttgccc tcgtggagca cgatgccgcc
     5821 tatgagctgc acgtcgaagg ggcgcatgcc cagcgtgcgg cgcgacacct ccctcaccgt
     5881 ggcgaaggcc tcgggcagga tgtcatcgag ggatgcaccc gcatccacca tctccttgaa
     5941 ccgtggggtc tgggccttga gctcggcgtc gctctttgcg cgcatcgtgt cttccaaggc
     6001 gttgatcgcc tccacgatgg gcctcatcct cttcagctcg cgatcgttct tgctcccgaa
     6061 cagcttgatg agccagttga tcatgtatgc gcttcccgcg tttcctttcg tgtcacaatg
     6121 cttccccgtg aggatgccgg agcatacctc atgacgaacc ctgtgcggcc tggtcggtct
     6181 tgtgaggata catagaacac ggatctgtgc atttcaacac gtatcttccc gtcactgcgg
     6241 agaaggaggc cgggccctgt ctactgcgcg ccaacgtcca ccgagtcgac gagtatggac
     6301 gggcagagca cgtgcccgaa ggcgcgcgca tcgtccccga cggcgatcac ccggccgagc
     6361 atgtcgaaga tgttgccggc gatggccgcc tccctcaccg ggtggacagg ctccccatgc
     6421 tccaggagga taccgttgat gcccaccgac atctcgccgg tgatggggtt tgccgtgtgc
     6481 atgcccatga tgtccatcac cttgagcacg gcgggtatcc ccgagaggac ttcccggagg
     6541 gggaccgtcc cgggcacgac ggagatgtgc ctgctcgcaa gcgtcggcca cgcgcggtag
     6601 ctcgacctga cggagttccc cgtcgaggag aggcctgagc gccttgccca gtacgcgtcg
     6661 tagagaaagg cgctgaccgt gccgcggtcg acgaggaccg tgcgcatgga gggcatcccc
     6721 tcgccgtcga aggggcagca gtccgccgca tgcacgtcca gggggtcgtc cacgatcgtc
     6781 aggcaggcgg agaagcgatc catgcccagg gaatccttca ggaaggattt ccccttgagc
     6841 acgttctcgc cgaggaagga ctcggagatg aagtcgaaga tctccgaggc gctcgaactg
     6901 tcgaagagca ccggcatgcg cctcgtggcg acgcgcctgg cgccgaggag ggcggccgcg
     6961 cgctcaacgg cgcgcctccc caccgcctcc acgtcgaatc cggagacaaa gtggctgaag
     7021 tcgaagtcgt agcccgactg aacgtcttcg ccccgcctca cc
//
LOCUS       DAOSCR010000052         7012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6917_length_7012_cov_4.175363, whole genome shotgun sequence.
ACCESSION   DAOSCR010000052 DAOSCR010000000
VERSION     DAOSCR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6917_length_7012_cov_4.175363"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(609..2309)
                     /locus_tag="PLT69_02395"
     CDS             complement(609..2309)
                     /locus_tag="PLT69_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOJ13328.1"
                     /translation="MARRFRSVGMVTAALLAVTLATPLGLGAAMYEQLVTSPLAVSMG
                     SAVTAYPPGAMAIHYNPAGLSKIPGTRFDNGIGFVSTYREVSLRQADDPATGKPWAPF
                     GGWFNNGRDPLDGAKDRQESGYMIIPYIDYAIPYLITPGMGISYKPPDPKFSRWTFGF
                     GQFAPYGAGLKNAKNPQKNPMSFLGEKAFFSRMSFLTPAVAYRVSDTLSVGAAVGIGP
                     SLFYLSSSLRTPNDMSALTGALGEATEGLEIPVISELTLPPPWFNGGMTPYATHGHLE
                     LFVEDYFTTSYNLGVLWEPYEWFALGACYQSSSEANMEGDYEFKYGSEFRRTVDWLGR
                     SPLTIITAAMFDLPTASVPKQKGTATFRLTWPQRLQLGVKLKPIKQLTLTCDANWTDW
                     AVQKQWVMQFDQKIQLFRFARMLGYRYSPDTQVFVHDFKNTWHLSYGMEIKPVEKFAL
                     RLGWDSRPASTVHERFGPVPFPDFDIYSLGIGIVVDDKPKPRPKDFHELTQQINHANA
                     IDINVSYIKLKDTTIKSNTSKSLNSTVFTDIVYNPYAGLDWHQEMHLWWFAVNQVFKW
                     "
     gene            complement(2323..3411)
                     /locus_tag="PLT69_02400"
     CDS             complement(2323..3411)
                     /locus_tag="PLT69_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOJ13329.1"
                     /translation="MEPGPFLKLKALFVLAVVAGLAVLPVPAFAEEKDKAAQGSQQEL
                     MERIEVLEKQIRRLEEEAKARRSLEITEEEKAEKEKEVLSAVSRDYTLDPKGTLALDY
                     SINYSYSPSENIYSQDQILRLERVNDHTIQHSIYTAYSVLDNFTASLNLPVMYRYAQR
                     GTDDELDETNIGDISLGAAFQPFKMEAGKVRYTLSMGAVLPTGRSPYKINPKSELSTG
                     NGLYSVSLGANFSKQIDPVVFFWGLGYSYPLDFNVDDLEYHVSDQLVLTRVKPGSTMS
                     ANMGMGYALSYANSINMSFSYSYQLSSRLTYNNARSIETGDRASASFGVGMGINVAPK
                     TTMSVSLGYSLTGSGFSLTARVPFNFVL"
     gene            complement(3587..4339)
                     /locus_tag="PLT69_02405"
     CDS             complement(3587..4339)
                     /locus_tag="PLT69_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOJ13330.1"
                     /translation="MEVLVAFVIVVTLMGTMYPVRDNPKETMLLYPGMTGVENLSLKQ
                     EVTPHEVFKDARITKQKYDYSCGSAALATLLNGYLRENLSEQQIIQGLMQYGEAEKIQ
                     QRRAFSLLDMKRFCEVLGYTAAGYKGDFEDLARLDKPAIVPIEISGYKHFVVFRGVYG
                     DHVFLADPYLGNTSYTVNRFKDIWPQKILFIVSSEGVKTNALALGREDLRIIEYDMTK
                     HAYTGVLPPDTVRRQHDFIESQGGRFYRTLFY"
     gene            complement(4373..5296)
                     /locus_tag="PLT69_02410"
     CDS             complement(4373..5296)
                     /locus_tag="PLT69_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13331.1"
                     /translation="MRSTGRLNSKKGCIPVAVGMAMAALVFVPACLLAMENLCDEALG
                     QVTAQAGITYNYGDFGVHVGIGSYRFSDTDSSPRNWIEFNNVTISGPGGYFLLDCPQD
                     YPMTIDVATSTTIDDQGLTFIEYQMSDHVNRRDWSVGNFVFCNQDLGSITFDTSTVDP
                     AVFRVSSHRETGSSGIDFEYLFRWRTQDFTYAYNTSGGSLHVAGLHLAETAGGDPSDP
                     STWTFTGPFRVGDLYGGTIDTDNDPSNQAHANPATFDVATVDDKTYVLVNLPLKGSIR
                     MEGVSMNGMDFGPVAIDGIKAHVLSIKINPE"
     gene            complement(5480..6472)
                     /locus_tag="PLT69_02415"
     CDS             complement(5480..6472)
                     /locus_tag="PLT69_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13332.1"
                     /translation="MTQATEDELSLVTGQAGVNINADLTMNIAIGTMAWGDASGIDTG
                     FAQFGWGTSDTTAGYVGVTGFNISNLRIKAREGDTYGTGYTAPGPSQVYNGTGAYGYS
                     TYMLKPITIDVGSDSTYVTYNGATFVRIGLGSLQVSMDAMGMNVALGSAGSSLSQVLG
                     SVNVGGMNLYVNPTSYVDIYNAKPGSARAVFFTFNLVFDRFDMDYLSWGDSDGAGTNV
                     GSGGVPWYVNSTTGYIGLDNLVVGAPIKVTGTVIIDVGTASTGVYAAHGPTTVVHIIF
                     QRPFTVSVGTITADVRLDSTAGFTGANAHTMGDIYLAGFRMTVQQGSWVDIWAH"
     gene            complement(6709..>7012)
                     /locus_tag="PLT69_02420"
     CDS             complement(6709..>7012)
                     /locus_tag="PLT69_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13333.1"
                     /translation="LIVGGPISITGTVRIDIGTATTGVYAAAAAAHGSTTQTTVVHIS
                     FADNFSFNVTGPITANVRLDSAAALNSEGAHTLGDIYISSFGVSIANGSWVDIWAH"
BASE COUNT         1190 a         2186 c         2255 g         1381 t
ORIGIN      
        1 cttgacgagc acgtccacct tctcgccgaa cgcgaaagca gcctgcgccc acgcaagggt
       61 caccacgaga accgcgcact gaaacgcacc ccgaaaacgc acgcaaccat tcgtcatctc
      121 tttgtctcct gtccttggca gtgttttctg aagatgcctc tcatccaacc gagatgtgtc
      181 tcgaacagat accctctata aacccggcgg gctcaaaact caacccatgt ctacgacggc
      241 gcgggcggac atagatcgca agccccccgt ccccaggtgg aactcgagac acgacacacg
      301 ttcacgacgg cgcggcaggg gcagggacct gggggagaca ccggggtatg ggtgaccggg
      361 ccccccgcga gggtatccgt gtgttcctgc atgcagggaa aacccgcacc ccccacatgg
      421 accccaccat tctctcggcc aagttcaccg gcctgcaggc acactggccg tggaaggccg
      481 cccccctgaa gattcggccc ccccgcgagg gtgtcctgct atgtctgccc gcgcctgcag
      541 ggggatatcg caggacgtat gaagcgtata gccggccaag tgtgtctgcc cgcgccgaca
      601 gggggatatc accacttgaa gacctggttg accgcgaacc accacaggtg catctcctgg
      661 tgccagtcga gcccggcgta ggggttgtac acgatgtccg tgaacaccgt ggagttcagg
      721 gacttgctcg tgttgctctt gatggtggta tccttgagct tgatgtagct cacgttgatg
      781 tcgatggcgt tcgcgtggtt gatctgctgg gtgagctcgt ggaagtcctt gggcctgggc
      841 ttcggcttgt cgtccaccac gatgccgatg cccagcgagt agatgtcgaa gtccgggaac
      901 gggaccgggc cgaagcgctc gtgcacggtg gacgcgggcc tcgagtccca gccgagcctg
      961 agggcgaact tctcgaccgg cttgatctcc atgccgtagg agaggtgcca ggtgttcttg
     1021 aagtcgtgga caaacacctg cgtgtcgggc gagtagcggt aaccgagcat cctggcgaag
     1081 cggaagagct gtatcttctg gtcgaactgc atgacccact gcttctgcac cgcccagtcc
     1141 gtccagttgg cgtcgcaggt gagcgtgagc tgcttgatgg gcttgagctt gacgcccagc
     1201 tggagcctct gcggccatgt gagcctgaag gtggcggtcc ccttctgctt ggggaccgac
     1261 gccgtgggca ggtcgaacat ggcggccgtg atgatggtga gcggagagcg gccgagccag
     1321 tcgacggtgc ggcggaactc gctgccgtac ttgaactcgt agtcgccctc catgttcgcc
     1381 tcggaggaac tctggtagca ggccccgagc gcgaaccact cgtagggctc ccagagcacg
     1441 cccagattgt aggaggtggt gaagtagtcc tccacgaaga gctccaggtg gccgtgcgtc
     1501 gcgtacgggg tcatcccgcc gttgaaccag ggcggcggaa gggtgagctc cgagatgacc
     1561 gggatctcga gcccctcggt cgcctcgccc agcgcgccgg tgagcgcgct catgtcgttg
     1621 ggcgtcctga gagacgacga caggtagaag agcgacggcc ctatgcccac cgcagcgccc
     1681 acggagaggg tgtcggagac gcggtaggcc actgcaggcg tcaggaagct catgcggctg
     1741 aagaacgcct tctcgccgag gaagctcatg gggttcttct gggggttctt cgcgttcttg
     1801 agccctgcgc cgtagggggc gaactggccg aagccgaagg tccagcggga gaacttcggg
     1861 tccggcggct tgtaggagat gcccatgccc ggggtgatga ggtaggggat cgcgtagtcg
     1921 atgtacggga tgatcatgta cccgctctcc tgccggtcct tcgccccgtc gagcgggtct
     1981 ctcccgttgt tgaaccaccc gccgaacggc gcccacggct tgcccgtggc cgggtcatcg
     2041 gcctgcctca ggctcacctc ccggtaggtg gacacgaacc cgatgccgtt gtcgaacctc
     2101 gtccccggga tctttgacag gcctgcgggg ttgtagtgga tggccatggc gccgggcggg
     2161 tacgcggtca ccgcgctgcc catggagacc gcgagcgggc tcgtgaccag ctgctcgtac
     2221 atggccgcgc cgagccccag cggtgtcgcg agcgtcacgg cgagcagcgc cgccgtgacc
     2281 atccccacgc ttctgaacct tctcgccatc tcctctgact cctcacagca cgaaattgaa
     2341 aggcaccctc gcggtcagcg agaacccgct tcccgtgagg ctgtagccga gtgaaaccga
     2401 catcgtcgtc ttgggcgcca cgttgatgcc catgcccacg ccgaagctcg ccgaggccct
     2461 gtcgccggtc tcgatggacc tcgcgttgtt gtaggtcagg cgcgaggaca gctggtagct
     2521 gtagctgaac gacatgttga tggagttcgc gtacgagagc gcgtacccca tgcccatgtt
     2581 cgcgctcatg gtcgaccccg gcttcaccct cgtcagcacc agctggtccg agacgtggta
     2641 ctcgaggtcg tcgacgttga aatcgagcgg gtacgagtac ccgaggcccc agaagaagac
     2701 caccgggtcg atctgcttgc tgaagttcgc gcctagcgac accgagtaga gcccgttccc
     2761 cgtggagagc tcgctcttcg ggttgatctt gtaggggctc ctcccggtgg gcaggacagc
     2821 ccccatggac agggtgtacc ggaccttgcc ggcctccatc ttgaagggct ggaaggcggc
     2881 gcccagggag atgtcgccga tgttggtctc gtcgagctcg tcgtcggtcc cccgctgggc
     2941 gtagcggtac atcacgggca ggttcaggct cgcggtgaag ttgtcgagca cgctgtaggc
     3001 cgtgtagatg gaatgctgga tcgtgtggtc gttcacgcgc tcgagcctca ggatctggtc
     3061 ctggctgtag atgttctcgc tcggtgagta ggagtagttg atactgtagt cgagggcgag
     3121 ggtccccttc gggtcgaggg tgtagtccct gctcacggcc gagaggacct ccttctcttt
     3181 ctccgccttc tcctcctcgg tgatctcgag gctcctcctg gccttggcct cctcttccag
     3241 cctcctgatc tgcttctcga ggacctctat gcgttccatc agctcctgct gcgagccctg
     3301 tgccgccttg tccttctcct cggcgaaagc gggcactggc aatactgcga gaccggcgac
     3361 gacagcgaga acgaacagcg ccttcagttt gagaaacggt ccgggttcca aggtacctcc
     3421 atcatcgaga agtctctcct gtcccatgcg cacacaacat atcgacattg cattcatttc
     3481 agcccggcgg cgtccgcctg tctcgaacag gcgccgacag ggtcgtgtgc gtggcagtat
     3541 accccatttc ggcgatgccg ccaagtctcg ggcatgccct gcaggatcaa tagaacaggg
     3601 tcctgtaaaa ccgccctcct tgcgactcga tgaaatcgtg ctgcctcctc acggtgtcag
     3661 gtggaaggac cccggtgtac gcgtgcttgg tcatgtcgta ctcgatgatc ctcaggtctt
     3721 cccggccgag ggcgagggcg ttggtcttga caccctcgct ggacacgatg aacaggatct
     3781 tctgcggcca gatgtccttg aacctgttca cggtgtagct cgtgtttccg aggtaggggt
     3841 ccgccaggaa cacgtggtcg ccgtagacgc ccctgaacac cacgaagtgc ttgtaaccgg
     3901 atatctcgat ggggacgatg gccggcttgt caaggcgggc gaggtcctcg aagtcgccct
     3961 tgtagccggc ggccgtgtat cccaggacct cgcagaacct cttcatgtcg agcagcgaga
     4021 aggcccttct ctgctggatc ttctccgcct ccccgtactg catgagcccc tggatgatct
     4081 gctgctcgct caggttttcc ctgaggtacc cgttcaggag cgtggccagc gcggcggagc
     4141 cgcagctgta gtcgtacttc tgcttggtga tccgcgcgtc cttgaacacc tcgtgcggcg
     4201 tgacctcctg cttgagcgag aggttctcca cccctgtcat gccagggtag agcaacatgg
     4261 tttccttcgg gttgtcgcgc accgggtaca tggtgcccat gagggtcacg acgatgacga
     4321 aggcgacgag cacttccatg gcgtcacgtt cctccggggt gcttcaaccc ggtcactcgg
     4381 ggtttatctt gatggagagc acgtgggcct tgatgccgtc gatcgccacc ggcccgaaat
     4441 ccatgccgtt catgctcacc ccctccatgc ggatggaacc cttgaggggg aggttgacga
     4501 gcacgtacgt cttgtcgtcg accgtggcca cgtcgaaggt cgccgggttc gcgtgagcct
     4561 ggttcgacgg gtcgttgtcc gtgtcgatgg tccccccgta gaggtcaccc acgcggaagg
     4621 ggccggtgaa cgtccacgtg gatgggtcgg acgggtcccc gccggcggtc tcggcgagat
     4681 ggagcccggc tacgtgaagg gatccacccg aggtgttgta cgcgtaggtg aagtcctggg
     4741 tgcgccacct gaacaggtac tcgaagtcta tgcccgagct ccccgtctcc cggtgggagg
     4801 agacgcggaa gacggccggg tccacggtgg aggtgtcgaa ggtgatggac cccaggtcct
     4861 ggttgcagaa cacgaagttc cccacgctcc agtcgcgccg gttcacgtgg tcggacatct
     4921 ggtactcgat gaaggtgagc ccctggtcgt cgatggtcgt cgacgtcgcc acgtcaatgg
     4981 tcatggggta gtcttgcggg cagtcgagga ggaagtaccc gccgggcccc gatatggtga
     5041 cgttgttgaa ctcgatccag ttcctgggcg agctgtccgt gtccgagaac cggtacgagc
     5101 cgatgcccac gtgcaccccg aagtcgccgt agttgtaggt gatgcccgcc tgggctgtca
     5161 cctggccgag cgcctcgtcg cagaggtttt ccatcgcgag caggcatgcg gggacgaaga
     5221 ccagggccgc catcgccatc ccgacggcga ccggtatgca gccttttttc gagttcagcc
     5281 tacctgtcga tctcatctct ctcgcctctt tcgccttccc atcgtgcggc aacgccgcct
     5341 cgctgagatt cctatcggcc gtgccccaaa acgtcttgaa ctcccgcaac gtttttcccg
     5401 cggggcttcc cgccgtccgc gccgttggtc ttcgccaccc gtctcgctct cgccagccct
     5461 tggaacggcc cgcacaagat cagtgggccc agatgtccac ccagctcccc tgctggacgg
     5521 tcatgcggaa gcccgccagg tagatatccc ccatcgtgtg ggcgttcgcg cccgtgaagc
     5581 cggcagtgga atcgagcctc acgtccgccg tgatggtgcc taccgagacc gtgaagggcc
     5641 gctggaagat gatgtggacc acggtggtgg ggccgtgcgc cgcgtacacg ccggttgagg
     5701 ccgtccccac gtcgatgatg accgtgccgg tgaccttgat gggagcgccc acgacgaggt
     5761 tgtcgagccc gatgtagccg gtggtgctgt tcacgtacca cggcaccccg cccgagccga
     5821 cattcgtccc cgcgccgtcg gagtcgcccc acgagaggta gtccatgtcg aagcggtcga
     5881 acacgaggtt gaaggtgaag aacacggccc tggccgagcc gggcttggcg ttgtagatgt
     5941 caacgtagct cgtggggttg acgtacaggt tcatgccgcc cacgttgacc gagcccaaga
     6001 cctggctcag cgatgagccg gcactcccca gcgcgacgtt catgcccatg gcgtccatgc
     6061 tcacctggag ggaaccgagg ccgatcctca cgaaggtggc gccgttgtag gtgacgtagg
     6121 tagagtctga gccgacgtct atggtgatgg gtttcagcat gtaggtgctg tacccgtagg
     6181 cgccagtgcc gttgtagacc tggctgggtc cgggcgcggt gtaccccgtc ccgtaggtgt
     6241 cgccctcccg ggccttgatc ctgaggttgg agatgttgaa accggtcacg cccacgtagc
     6301 cggccgtggt gtcagacgtg ccccatccga actgggcgaa cccggtgtcg atgccggagg
     6361 cgtctcccca cgccatggtg ccgatggcga tgttcatggt gaggtctgcg ttgatgttga
     6421 cgcccgcctg gccggtcacc agggagagct cgtcctcggt cgcctgcgtc atggcctcga
     6481 ggcccgaggg cgcggaaaga caaaggatga acgatatgat ggctatgaat agtgcgcctg
     6541 acgatacatg agcgtctcga gcctgttcac caagcattgt cctcgacccc ttcattgctg
     6601 cggtctcctt tcaccccatg atgcgattcc atcctcatga cattccacca agaaacgaga
     6661 aaaatcgagg agaccgggga ttaccccggc ctcctcgagc gttacgaatt agtgggccca
     6721 gatgtcgacc cagctgccgt tggcgatcga cacgccgaag ctggagatgt agatgtcacc
     6781 cagcgtatgg gcgccctcgc tgttgagggc agccgcgctg tcgagcctca cgttcgccgt
     6841 gatcggaccg gtcacgttga agctgaagtt gtcagcaaag ctgatgtgga ccaccgtggt
     6901 ctgcgtggtc gacccatggg ccgcggccgc ggccgcgtac acgcccgtgg tcgcggtgcc
     6961 gatatcgatc ctcaccgttc cggtgatgga gatcgggccg ccgacaatca gg
//
LOCUS       DAOSCR010000053         6958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_6983_length_6958_cov_3.373026, whole genome shotgun sequence.
ACCESSION   DAOSCR010000053 DAOSCR010000000
VERSION     DAOSCR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6983_length_6958_cov_3.373026"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="lgt"
                     /locus_tag="PLT69_02425"
     CDS             <1..668
                     /gene="lgt"
                     /locus_tag="PLT69_02425"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ13334.1"
                     /translation="IAEHAYPYRDGADVEDLVTYCILGLVAGARIGYCLIYNPSYYLA
                     HPLKFFAVWEGGMSFHGGLIGLVLTGYLFSRARKKPFLMLADLGAIGATPGLFFGRIG
                     NFINGELFGRVTDAPWGMVFPSGGPVARHPSQLYEAFGEGLLLFVILYLAARKVSTHG
                     VVFGIFLACYGAVRFVLEFFREPDPQIGFVLLTLTMGQVLCLAMVALGLFTVGVSLKS
                     RPR"
     gene            685..2583
                     /locus_tag="PLT69_02430"
     CDS             685..2583
                     /locus_tag="PLT69_02430"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HOJ13335.1"
                     /translation="MGSHDQSITRALREFSRYNALKKRRMYDCDPVSARVILEFLPLL
                     LHINHPALPGFVDDACPCGIMRFDWGPEKMRETGAFFSSRLKVGDLRQFLPDRRLIEG
                     IYTIGSSGSLAQTRESDFDVWVVVDSEKTGKDGMEMLRRKIRMIQRFISTKFVLDIHF
                     FLMDLEDIRKNDFGSVSQEGSGSALKTILKEEFYRTMTIVEGCVPLWWVVQAGRGRDA
                     YEEARRAVLTKKEDAGRIFVDMGDLQGIPEHELMGAALWQMHKALDDPLKSVLKMALL
                     VSYLDASGVPELLCDRLKREVHENAAGRVIDPYIHVLRRLEEHYLEQDDPKTVDLLRK
                     CFYLKVNPQIKPMDLVKAGGEDKAAAMAEVVHSWGWSMHAVKNLNGFSQWGVERYRVF
                     GDEIHSFLRQSTVKIIRSARSFMVSGTSIDDDIEVEVLRRRIEAFYVTKKGKIEAERR
                     VKKREPAYRDIYFAYGKGRWCIYDSAPEAGQRPIVEAKRVVGVLAWLVYNKRFDASTS
                     FHMVPNPTGVALSDIQSLMKTLSAVIPDAASIGLDRTSLMEDRYPAIMVVIGNMECPE
                     VRHHIREIDVVYMNTWKELFCEYMSPDHLKGWVKRTRRPGTEIRIWLPKEAVTSGLTF
                     NLSRLLSP"
     gene            2630..3496
                     /locus_tag="PLT69_02435"
     CDS             2630..3496
                     /locus_tag="PLT69_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOJ13336.1"
                     /translation="MELQDRTRRSSDRRLSVGICQLRQGYDVEANIERACELIGLASG
                     DGADICVLPEMFSTPYEPVSIRRAAALSASALERVCRKASELGVYVVAGSLPWPSGDG
                     RLYNRAFVVDPKGEAVYHHDKIHLFDCAPCGGPNVRESETVAPGSSLGSFTTPWCRAS
                     VIVCYDVRFVQLVQVLADMGVRLLFVPAAFSSATGEAHWEMLVRIRALELQGYVVGVQ
                     PAVNEDLAYVPWGRSVLASPWGEVLARAGTDEEVIVTEIDMTYTDEIRSRFPLLAHRR
                     ADLYETRWKGKG"
     gene            3493..4242
                     /locus_tag="PLT69_02440"
     CDS             3493..4242
                     /locus_tag="PLT69_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13337.1"
                     /translation="MIEIRGLTKTFKSQKVLDGLDLDIVAGKITIVMGPTGTGKSVLL
                     KHVLGLLKPDSGSILVEGRDIVAMDEAELTEVRKAYGVCFQDSALFDSMTVGENVGFP
                     FTIHTKLSREEIMREVSTLLREVGLAGIEAKMPAQLSGGMRKRVGLARALALSPKVLL
                     FDEPTTGLDPVMTTAINTLIRQVQQKTGATSLVISHDIKGAFSLADYMALLYNGKIAF
                     YGTPEDFRNTDDELVRQFVEGKLTGPINPIQ"
     gene            complement(4583..5041)
                     /locus_tag="PLT69_02445"
     CDS             complement(4583..5041)
                     /locus_tag="PLT69_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ13338.1"
                     /translation="MVGKILIPTDFSKTSRHAFRYAVSLNGTLKARLFLLHVINDFAD
                     LTEYSLSLSFLPQIYSELEHTATLKLEEMVTDMVPSDMHCDTYILHGIPFYEIVSFAL
                     SEQMDLIVMGSHGHTGLKHVLFGHTAEKVVKKAHCPVLTVKDPDCGLSLP"
     gene            complement(5379..6161)
                     /locus_tag="PLT69_02450"
     CDS             complement(5379..6161)
                     /locus_tag="PLT69_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13339.1"
                     /translation="MLAAFARNAVVEDDETGDEYPIAWSMVNWCCEERTLSLQYALAR
                     ALRSGMGAPVVMRTQELSSDHILGLVTGDPLPFASINLAGPLACEQTILEPGGAPVEG
                     DPQRLFWHIHHAVAMNVDGQLMVLDLSMADEPVSIQAWISSFVQDASACRHLGDPDFQ
                     KVEAYWRCVMSGMDPGPEPPCECAYTITPAFTWRFDQDVLVEAIQGVPPTLVVQSGAF
                     TTLMQDSYGVDVSRDDIPRYLSLYEAKTMEDVCTWVDLPFCR"
BASE COUNT         1304 a         2097 c         2292 g         1265 t
ORIGIN      
        1 cgatcgccga gcatgcctat ccgtatcgcg acggcgccga tgtcgaggac ctcgtgacgt
       61 actgcatcct cggcctcgtc gcaggggccc gcatcgggta ttgcctgatc tacaacccgt
      121 cctactacct ggcccacccg ctcaagttct tcgccgtgtg ggaagggggg atgtcgttcc
      181 acggcgggct catagggctg gtattgacgg ggtacctgtt ttcgagggcc aggaaaaagc
      241 cctttctcat gctcgcggac ctgggcgcca tcggcgcgac cccggggctg tttttcggcc
      301 gtatcgggaa cttcatcaac ggtgaactct tcggccgggt gacggatgcc ccgtggggca
      361 tggtcttccc gtccggcggc ccggtcgcac gccacccgtc gcagctctac gaggcgttcg
      421 gcgagggcct gcttctcttc gtgattctct acctggcggc gaggaaggtg tccacccacg
      481 gggtggtgtt cggcatcttc ctcgcctgct acggcgcggt gaggttcgtc ctggagttct
      541 tcagagagcc tgacccccag atcggcttcg tcctcctcac gctcaccatg gggcaggtct
      601 tgtgcctggc catggtcgcg ctgggtctgt tcactgtcgg cgtgtcctta aagtcacgcc
      661 cccgatgaag cgatagtctt tcagatgggg agccacgatc aatcgatcac tcgtgcgctc
      721 agggaattct cgcggtacaa cgccttgaag aagaggcgca tgtatgactg cgaccccgtg
      781 agcgcgaggg tcatcctgga gttcctgccg ttgctcctgc acatcaacca ccctgcattg
      841 cccggcttcg tcgacgacgc atgcccgtgc gggatcatga gattcgactg ggggcctgag
      901 aagatgaggg agacgggtgc gttcttctcc tcccgtctca aggtgggcga cctgaggcag
      961 ttcctccccg acaggaggct gatcgagggg atttacacga taggctcttc gggaagcctc
     1021 gcccagacca gggagtcgga cttcgacgtg tgggtcgtgg tggactcgga gaagacgggc
     1081 aaggacggca tggagatgct ccgcaggaag atccgcatga tccagcggtt catctcgacg
     1141 aagttcgttc tcgacatcca ttttttcctg atggacctgg aagacatccg gaagaacgac
     1201 ttcggctccg tatcccagga aggctctggc tcggccctca agaccatcct caaggaggag
     1261 ttctaccgca ccatgacgat cgtcgagggg tgcgtgccct tgtggtgggt ggtgcaggcg
     1321 ggccgggggc gcgatgccta cgaggaggcg cgaagggccg tcctgacgaa gaaggaagac
     1381 gccgggagga tattcgtgga catgggggac ctccagggga tccccgagca cgagctcatg
     1441 ggtgccgccc tgtggcagat gcacaaggcg ctggacgatc ccctgaagtc ggtcctcaag
     1501 atggccctcc tcgtgagcta cctggatgct tccggtgtcc cggagctctt gtgcgatcgt
     1561 ctcaagaggg aggtccacga gaacgcggct ggtcgcgtca tagacccgta catccacgtg
     1621 ctcaggcgtc tcgaggagca ctacctcgag caggacgacc ccaagaccgt ggacttgttg
     1681 agaaagtgct tctacctcaa ggtaaacccg cagatcaaac ccatggacct cgtcaaggcg
     1741 gggggtgaag acaaggccgc ggccatggcg gaggtggtgc attcatgggg atggtcgatg
     1801 cacgcggtga agaacctcaa cgggttctcg cagtggggcg tcgaacgtta ccgggtcttc
     1861 ggcgacgaga tccactcctt cctcaggcag tcgaccgtga agatcatccg cagcgcccgg
     1921 tcgttcatgg tctcgggaac gagcatagac gacgatatcg aggtcgaggt cttgaggaga
     1981 cggatcgagg ccttctacgt gaccaagaag gggaagatag aggccgagcg acgggtgaag
     2041 aagagggagc ctgcctaccg cgacatatac ttcgcctacg gcaaggggag gtggtgcatc
     2101 tacgattcgg cgcccgaggc cggccaaagg ccgatcgtcg aggcgaaacg ggtggtgggc
     2161 gtcctcgcgt ggctcgtgta caacaagcgg ttcgatgcat ccacctcctt ccacatggtt
     2221 ccgaacccaa caggcgtggc gctgtcggac atccagtccc tcatgaagac gctctccgcc
     2281 gtcataccgg atgccgcgtc catcggcctc gaccgcacct ccctcatgga ggaccgctac
     2341 cctgcgatca tggtcgtgat agggaacatg gagtgcccgg aggtcaggca ccatatccga
     2401 gagatagacg tggtgtacat gaacacgtgg aaggagctct tctgcgaata catgagcccg
     2461 gaccacctca aggggtgggt gaagaggacg aggaggcctg gcacggagat caggatctgg
     2521 ctccccaagg aggcggtcac ctccggcctc accttcaacc tgtcccggct cctctcccct
     2581 tgactggcgg gggttcatgc cgatacaact gcggacaggt ctttcgcaca tggaactcca
     2641 agatcgcacg agaaggtcct ctgatcgaag actgtcggtc ggtatctgcc agctgcgcca
     2701 gggatacgac gtcgaggcga acatcgagag ggcgtgcgag ctgatcgggc tggcatccgg
     2761 agacggcgcc gacatctgcg tgctccccga gatgttctcc accccgtacg agccagtctc
     2821 gatcaggagg gcggccgccc tgagcgcgtc cgccctcgag cgcgtgtgca ggaaggcctc
     2881 ggaactcggc gtctacgtgg ttgcgggctc gctgccctgg ccctcgggcg acgggcgtct
     2941 gtacaaccgg gccttcgtgg tcgacccgaa aggggaggcg gtataccacc acgacaagat
     3001 ccacctcttc gattgcgccc cgtgcggggg gccgaacgtg agggaatcgg agaccgtggc
     3061 gcccgggtct tccctcgggt ctttcaccac cccctggtgc agggcatcgg tgatcgtgtg
     3121 ctacgacgtg aggttcgttc agctcgtgca ggtcctggcg gacatgggcg tcaggcttct
     3181 cttcgtgccg gccgccttct cctcggcgac cggcgaggcg cactgggaaa tgctcgtgag
     3241 gatcagggcg ctggaactcc agggctatgt cgtgggcgta cagcctgcgg tcaacgagga
     3301 tctcgcgtac gtgccctggg ggcgctcggt tctcgcgtcg ccctggggcg aggtcctggc
     3361 aagggccggg acggacgagg aagtgatcgt gacggaaatc gacatgacgt ataccgacga
     3421 gatcaggtca cgattcccgc tgctcgccca caggagggcg gacctctacg agacgcggtg
     3481 gaaggggaag ggatgatcga gatacggggg ctcaccaaga cgttcaagtc tcagaaggtg
     3541 ctcgacgggc tcgacctcga catcgtggca gggaagatca ctatcgtgat gggcccgacg
     3601 gggaccggca agagcgtgct cctcaagcac gtgctcggcc ttctgaagcc cgattccggc
     3661 agcatcctcg tggagggccg ggacatcgtg gccatggacg aggctgagct caccgaggtg
     3721 aggaaggcat acggggtctg cttccaggac tcggcgctct tcgactccat gaccgtaggc
     3781 gagaacgtgg ggttcccctt caccatccac acgaagctct cccgggaaga gatcatgagg
     3841 gaggtgtcga ccctgctcag ggaggtgggg ctcgcgggca tcgaggccaa gatgcccgcg
     3901 caactctccg gcggcatgcg caagagggtg ggcctcgcga gggccttggc gctcagcccg
     3961 aaggtgctcc tgttcgacga gccgaccacc ggcctcgacc cggtcatgac gacggccatc
     4021 aacaccctca tcaggcaggt gcagcagaag acgggcgcca ccagcctcgt catcagccat
     4081 gacataaagg gtgccttttc gctggcagac tacatggcgc tcctctacaa cgggaagatc
     4141 gcgttctacg gtacgcccga ggacttcagg aacaccgacg acgagctcgt caggcagttc
     4201 gtggaaggca agctcacggg gcccatcaac cccatccagt gaatgcaagg gcccgctggg
     4261 gcaggggggc tgcgtttcgc accgtggcct ggcccggttc cacggcctcg aacccacgcc
     4321 gcggcgcgaa agccgtgtga gaggcctggg cgggggaaaa accgcccgag gccggttcgt
     4381 atcgagcaag aacaatccgt accgtaggcg gccctgtcca tcgatggaag agaatgaacc
     4441 cttaggccat ggaccccccc atgccgcccg gcgcaaaagc ctcggactct cacgaaaccc
     4501 gcgtgcggca tcgcaggaat atcccggggg caggatgggg tatgtacggg ctaaggggtc
     4561 gcccgcgcgg ccttgcgggc gcctagggga gagagaggcc gcaatcaggg tccttcacgg
     4621 tcagcacagg gcagtgcgcc ttcttgacca ctttctcggc ggtatggccg aacaatacgt
     4681 gcttgaggcc cgtgtgcccg tgagagccca tgacgatgag gtccatctgc tcgctcaagg
     4741 cgaagctcac gatctcgtag aaagggatgc cgtgcaggat gtaggtgtcg cagtgcatgt
     4801 ccgagggcac catgtcggtc accatctcct cgagcttcag ggtggccgta tgctccagct
     4861 cgctgtatat ctggggaagg aacgacaggc tgaggctgta ttcggtgagg tcggcgaagt
     4921 cgttgatgac gtggaggagg aagagcctcg ccttgagtgt tccgttgagg gagaccgcgt
     4981 accggaacgc atgcctggat gtcttgctga agtccgtagg tatgaggatc ttccccacca
     5041 tttggcagta taaataatcc cgcgatcggc agagtcaata catgtcctcc cgcaccacga
     5101 ggcatgatgg agagggattt ctcctgattt ccagccatta tcggccgtgg ttgcgggtca
     5161 tcgcccgtgc cgtgggccga gatttcccat ggtgcaggtc accatcggcg ccgagagggt
     5221 gcgtgcgcca aggaacacgg cccgggcatg tcccagcggc acgcgctgtg tcttccttgg
     5281 cgggcagaat gggctgaccg cccggggatt gcgggcatgg gtcttctctt cggcgtgtgt
     5341 tcgatgacaa gagaaaatgg aaggtcctat gagtcggcct acctgcagaa aggaaggtca
     5401 acccacgtgc acacatcctc catggtcttg gcctcgtaca ggctcagata cctcggtatg
     5461 tcgtctctcg acacgtccac cccatagctg tcctgcatga gggtggtaaa ggcaccgctc
     5521 tgaacgacaa gggtcggcgg aacgccctgt atcgcttcga cgaggacatc ctggtcgaag
     5581 cgccaggtga acgcgggggt gatcgtgtac gcgcactcgc agggcggctc ggggccggga
     5641 tccatgccgc tcatgacaca gcgccagtac gcctcgacct tctggaagtc aggatcgccg
     5701 aggtgcctgc atgccgaggc gtcttgcacg aagctcgaga tccacgcctg gatgctcacg
     5761 ggctcgtcgg ccatggagag gtcgaggacc atgagctggc cgtccacgtt catcgccacg
     5821 gcgtggtgga tgtgccagaa gagccgttgg gggtcaccct cgacaggagc gccgccgggc
     5881 tcgaggatcg tctgttcgca ggccagtggc cctgcaaggt tgatcgaggc gaaggggaga
     5941 gggtccccgg tgacgagacc caggatatgg tcgctcgaga gttcctgggt cctcatgacc
     6001 acgggggcgc ccatgccgga acgcaaagcg cgggcgaggg catactggag ggacagagtg
     6061 cgttcctcgc agcaccagtt caccatggac catgcaatgg ggtactcgtc cccggtctcg
     6121 tcgtcttcga ccacggcgtt ccgtgcaaag gccgcgagca cgagcgcctc gtcacgttga
     6181 atggggctca gcctcggtgt gacgggtgaa ccgtcggaag aatcgttgtg gcagcaaagg
     6241 catgcgaggc agaccaaacc gaacgcagcg actcttcgcg aaaaggacgt tgcgcgcctt
     6301 cggccttcga ggagggacaa cggaaggata ggtgcgttac gcatgaaaga cctccttgct
     6361 ccaagctcaa ggaccaccca tccgacgggt ggtccacggg tcaccctttc atcacacgca
     6421 gggcgggcgc tccacgcgcc aggggaaacc atcggctcgt cacggccgga caacctcccg
     6481 gaaacgcctt cacggcaaac tctccgcata ccatcggcgg cccgtcacgt gaagctccag
     6541 gcacccctgc ccccatcggc gagacatgcc ctttcacgcc tcaacgagcc attgaacgtt
     6601 ctcttgcgcc cgcggcccag gttcggccga caaccggcct cgtgcgccac atcgcacgca
     6661 tggtcattct atcacccgtc gcgccaacac tcaacctgta tccggtatgt tacgagcagc
     6721 accgggccgg gcgacaggct ccgggggtgc gttcgccttc gcacgaacgt cgcacgtgcg
     6781 cgtttgccgg ttccgtcccg cggtttctga aggggggatc ctctcgatac ggtgcacctc
     6841 ggccaaggcg gtccttgttt cccaagggaa acccatgggg tggtcgttca cgcacagggg
     6901 catgtcgtct tggcggtccg cctgaggggc ttccgtcggg atgggttccc gatcgtgc
//
LOCUS       DAOSCR010000054         6936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7021_length_6936_cov_2.873565, whole genome shotgun sequence.
ACCESSION   DAOSCR010000054 DAOSCR010000000
VERSION     DAOSCR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7021_length_6936_cov_2.873565"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1066)
                     /locus_tag="PLT69_02455"
     CDS             complement(59..1066)
                     /locus_tag="PLT69_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOJ13340.1"
                     /translation="MAGEPLPLGKLPIPLLRRFLEEVGKNHPDVVVGPGIGVDAAVIR
                     FGETMLVFKTDPITFATDDIAWYLVTVNANDICCMGGIPEYLLVTMLLPRGSTTEQSV
                     HDLFVSLKRACDANEVTLVGGHTEVTHGIDRPIAVGFMVGSLSQHGVVRASDAGPGDV
                     ILMSKAVPIEAVSLLAREFPHRLDIDEETLAKARNLIYDPGISVRKEALIAMSVGGVT
                     AMHDPTEGGIATGLYELACATRCGIRIDGDAIPVIEHARAILPVFGMDPLGALASGTL
                     LVCCRPESAQRILDAWAKAGIPGTLIGTLTARQEHTIVRQGKQQELTAFDADEVTKAF
                     S"
     gene            complement(1056..1835)
                     /gene="folE2"
                     /locus_tag="PLT69_02460"
     CDS             complement(1056..1835)
                     /gene="folE2"
                     /locus_tag="PLT69_02460"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOJ13341.1"
                     /translation="MKDVQKLMPENPLRIEKVGVKGISYPIVVSDRKKGTQHTVASIN
                     LYVDLPRHFKGTHMSRFIEVLNEHREEIHIKNFENILEEIKRALEAESAHMELEFPYF
                     IEKTAPVSKAQGLMEYSCSLVGMVGPRREFKVGVKVPVQTVCPCSREISDFGAHNQRG
                     IVTVVVRYERFFWIEDLIELVESCGSSPVYPILKRPDEKYITEQAYNNPRFVEDVVRQ
                     AAFLLSRKSEFSWFSVEAENFESIHNHSAYAYIESELGHGR"
     gene            complement(1832..2218)
                     /gene="queD"
                     /locus_tag="PLT69_02465"
     CDS             complement(1832..2218)
                     /gene="queD"
                     /locus_tag="PLT69_02465"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOJ13342.1"
                     /translation="MFKVKVLTTFSAAHHLRGYRGDCERLHGHNYRVEVAVASREPGP
                     TGLVMDFRELKDVLESVVERLDHRLLNELDYFKEKNPSAENIARYIFEAVGKALGSEV
                     SLAEVTVWENDTCCVTCTPGPQDRSP"
     gene            2720..3664
                     /locus_tag="PLT69_02470"
     CDS             2720..3664
                     /locus_tag="PLT69_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOJ13343.1"
                     /translation="MPTIEFADTHILRQLAGDSCSHLKILEESTGVKCSLKGNTIELS
                     GESFSVELAGHALRDLYRLIKGGFPVYQDDVWYAVRMLAKDRTVDLEEVFKDSIYISS
                     TKKVIVPKSLQQKRYIDAMRVKDILFGIGPAGTGKTYLALAMGVHYLLKKKVERIVLT
                     RPAVEAGERLGFLPGDLQEKVNPYLRPLYDALYDMLERDHVLRLLARDTIEIAPLAFM
                     RGRTLNDAFIILDEAQNTTSEQMKMFLTRIGFNSKALITGDVTQIDLPPGVMSGLVEA
                     SRILKDIDELGFVYFTEDDVVRHKVVAKIIRAYEKNGR"
     gene            3661..4110
                     /gene="ybeY"
                     /locus_tag="PLT69_02475"
     CDS             3661..4110
                     /gene="ybeY"
                     /locus_tag="PLT69_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ13344.1"
                     /translation="MNLIDVIDEVGRNLVEADQVGRWAQEALAALGKEGVELCVVLVG
                     DERIRELNRDYLGRDRPTNVISFPQQEGEGPAGNHLGDVVISVERAAEEARDAHMSTN
                     ERIRQLLVHGICHLAGFNHEDVTEEEALAMEEAERMVLHAISGGKNE"
     gene            4801..4878
                     /locus_tag="PLT69_02480"
     tRNA            4801..4878
                     /locus_tag="PLT69_02480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4836..4838,aa:Pro,seq:tgg)
     gene            4895..4971
                     /locus_tag="PLT69_02485"
     tRNA            4895..4971
                     /locus_tag="PLT69_02485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4929..4931,aa:Arg,seq:tct)
     gene            4983..5058
                     /locus_tag="PLT69_02490"
     tRNA            4983..5058
                     /locus_tag="PLT69_02490"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5016..5018,aa:His,seq:gtg)
     gene            5075..5151
                     /locus_tag="PLT69_02495"
     tRNA            5075..5151
                     /locus_tag="PLT69_02495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5109..5111,aa:Arg,seq:tcg)
     gene            5174..5249
                     /locus_tag="PLT69_02500"
     tRNA            5174..5249
                     /locus_tag="PLT69_02500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5207..5209,aa:Lys,seq:ctt)
     gene            complement(5358..>6936)
                     /locus_tag="PLT69_02505"
     CDS             complement(5358..>6936)
                     /locus_tag="PLT69_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13345.1"
                     /translation="TSPSQPSGSTGSTQATNPAGAATSNPSSSVASSGDTSPNQSADP
                     DPGDADDTQSPPETLPRPVVVSPLDNEADVELTPLLEATPPPGDHWYGQWEISSASGD
                     LVLAATVASPTDGFQVPDLVLEGGTEYYCRVRYHDGSGTYTEWSEPTLFITARDDTED
                     LDGNGVPDDQEPPAGARVDLDRNGVSDLVQEGMLTILTTEGGMPLSLKASTNCRGIKV
                     FKALDTARGEGFETKPAYLPIGIVDFKMELDRPGDLAEVIVYLPRKAPVGSKWYFFDQ
                     RNGWREYPHAVLSEDRLSVTLTLKDGDPDYGDSDGAANGVIVDPGGLGVDGEAASFDE
                     SPRDQHLGCFVTATDTDRACPARTRRLGTSLPECSWLSVQRSARDEGGPPLRPGVCAG
                     LPPGPLRGRSPRGGSHPCTRGDGGHTGIFSPPWARPFNRRSCARRPVYKASPVTGIGK
                     GSPACTEASGRFERPRDRPVTRRRPDRASGGGHALVFPSDEPGEWPERVTPPGACVLE
                     CRREKGRMPANTNPDRA"
BASE COUNT         1204 a         2145 c         2304 g         1283 t
ORIGIN      
        1 ggaacctggg gccttggacg ccaggcctcc acccgcatgg aagagccgtg agggtgcttc
       61 aggagaaggc ctttgtcacc tcgtccgcgt cgaaggcggt gagctcctgt tgcttgcctt
      121 gcctgacgat cgtgtgctcc tgcctggcgg tgagcgttcc gatgagcgtg cccgggatgc
      181 cggccttggc ccaggcatcg aggatgcgtt gagcgctttc gggcctgcag catacgagca
      241 gcgttcccga tgcgagcgcg cccaacgggt ccatgccgaa gacagggagg atggcgcggg
      301 cgtgttcgat cacgggtatc gcgtcgccgt cgatccgtat gccgcaccgc gtggcgcagg
      361 cgagctcgta gagcccggtc gcgatgcccc cctcggtagg gtcgtgcatg gcggtgacgc
      421 cccccacgct catggcgatg agcgcttcct tcctcacgct tatccccggg tcgtaaatga
      481 ggtttcgagc cttcgcgagc gtctcctcgt cgatatcgag cctgtggggg aactcccgag
      541 cgaggagcga gacggcctct atggggacgg ccttgctcat gaggatcacg tctcccggtc
      601 ccgcatccga cgcgcggacc accccgtgct gcgagagcga gcccaccatg aaccccacgg
      661 ctatgggcct gtcgatgccg tgcgtgacct cggtatgccc gcccacgagc gtgacctcgt
      721 tcgcgtcgca cgccctcttc agggagacga acaggtcgtg gacgctctgc tctgtcgtgg
      781 agccgcgcgg caggagcatg gtgacgagga ggtactcggg gatgccgccc atgcagcaga
      841 tatcgttggc gttgacggtg acgaggtacc aggcgatgtc gtcggtggcg aaggtgatgg
      901 ggtcggtctt gaagacgagc atggtctcgc cgaacctgat cacggccgcg tccaccccga
      961 tgccaggacc gacgaccacg tcggggtggt tcttgccgac ctcctccagg aatctcctca
     1021 gcagcggtat ggggagcttc cccagcggca ggggttcacc ggccatggcc cagctcgctt
     1081 tcgatgtatg cgtatgcgct gtggttgtgg atgctctcga agttctcggc ctcgaccgag
     1141 aaccacgaaa actcgctctt tcggctcagg agaaaggcgg cctggcgtac cacgtcttcc
     1201 acgaacctgg ggttgttgta agcctgctcg gtgatgtact tctcgtccgg tctcttgagg
     1261 atcgggtaga cggggctcga cccgcacgac tccacgagct cgatgagatc ctctatccag
     1321 aagaagcgct cgtagcggac caccacggtc acgatgccgc gctggttgtg cgcgccgaag
     1381 tcgctgatct ccctggagca ggggcacacg gtctggaccg ggaccttcac gcccaccttg
     1441 aattcacgtc tcgggccgac catccccacg agggagcaac tgtactccat gagcccctgg
     1501 gccttgctca caggcgccgt cttctcgatg aaatagggga actccagttc catgtgcgcg
     1561 ctttcggcct cgagcgccct cttgatctcc tcgaggatgt tctcgaagtt cttgatgtgg
     1621 atctcctcgc ggtgttcgtt gagcacctcg atgaacctgc tcatgtgggt gcccttgaag
     1681 tgcctcggga ggtctacgta caggtttatc gacgcgaccg tgtgctgggt gcccttcttc
     1741 ctgtcggaca ccacgatggg gtagcttatg cccttgaccc ccaccttctc gatcctcagg
     1801 gggttttcgg gcatgagttt ctggacgtcc ttcatggcga tcgatcttgg ggcccgggcg
     1861 tgcacgtgac gcagcaggtg tcgttctccc agacggtcac ctcagcgagc gagacctccg
     1921 aacccagggc cttccccacg gcctcgaaga tgtacctcgc gatgttctcc gccgaggggt
     1981 tcttctcctt gaagtagtcg agttcgttga gcagccggtg gtcgagcctt tcgacgacgg
     2041 actcgagcac gtccttgagc tcgcggaagt ccatgaccag gcccgtcggg cccggctccc
     2101 tcgacgcgac cgcgacctcg acccggtagt tgtggccgtg gagccgctcg cagtcgcctc
     2161 ggtaccccct caggtggtgc gccgcgctga aggttgtgag cactttgacc ttgaacatgc
     2221 cgagtgtcat aatacatcgt tcatggcgtg gcaacatgca tttccatccg ggaatcgggg
     2281 tcctgccgac tccacgggcg ggcctccggg gtcggcgtga aggggtgcgc gggaaacggg
     2341 cgatcccggt attcccataa ggggccctgc ggccgtgctc gcattgccgg tcatgctaaa
     2401 caaatcgctc agccttccgg gattcccata agaatccctg cggccgtgcg cgggtgagac
     2461 gggccgaggg ctccatgcga cctcgcatcc cggtcagggt gccgcgcctg cacgcggggc
     2521 ttcggacagc gccagggtct cgatgccctg catcccgcct ggagggggag agacgggatg
     2581 caggaccctg gacattcccg ccctcacccc gcacaagtga tgagccgtgc gcctcgaggg
     2641 gtgctgatta cgcttgtgcg cggtgatgaa ttgagctatc cagagagtcg agggagggat
     2701 cgccccgctc ggaaacgaca tgcctaccat agaattcgca gacacgcaca tcctccggca
     2761 gcttgccgga gacagttgct cgcatctgaa gatcctcgag gaatcgaccg gtgtcaagtg
     2821 ctccctcaag ggcaacacca ttgagctctc gggcgagagc ttcagcgtgg agctcgccgg
     2881 gcacgcgctg agagacctct accggctcat caagggcggg ttccccgtct accaggacga
     2941 tgtctggtac gccgtgcgca tgctagccaa ggacaggacc gtggacctcg aggaggtctt
     3001 caaggacagc atctacatct cctccaccaa gaaggtgatc gtccccaaga gcctgcagca
     3061 gaagcggtac atcgacgcca tgcgcgtgaa ggacatcctc ttcggcatcg gccctgcggg
     3121 cacggggaag acctacctcg ccctcgccat gggtgtgcac tacctgctga agaagaaggt
     3181 cgagcggatc gtgctcacga ggccggccgt cgaggcggga gagaggctcg gttttctgcc
     3241 gggcgatctc caggagaagg tcaacccgta cctgagaccc ctgtacgatg cgctgtacga
     3301 catgctggag cgagaccacg tgctcaggct gttggcgcgg gacaccatcg agatcgcccc
     3361 cctggccttc atgaggggcc gcaccctcaa cgacgccttc atcatcctcg acgaggcgca
     3421 gaacaccacc tcggagcaga tgaagatgtt cctgacccgc atcgggttca attcaaaggc
     3481 gctcattacc ggggacgtca cgcagataga cctcccgccc ggcgtcatgt cggggctggt
     3541 cgaggcgtcc aggatcctca aggacataga cgagctgggc ttcgtctact tcaccgagga
     3601 cgacgtggtg cgtcacaagg tggtggccaa gatcatcagg gcctacgaga agaacgggag
     3661 atgaatctca tagacgtcat cgacgaggtc ggcaggaacc tggtcgaggc ggaccaggtg
     3721 ggcaggtggg cgcaggaggc gctggccgct ctcggcaagg agggggtcga gctgtgcgtc
     3781 gtcctcgtgg gcgacgagag gatcagggag ctcaacaggg actacctcgg ccgggaccgg
     3841 cctaccaacg tcatcagctt tccccagcag gaaggcgagg ggccggcggg aaaccacctg
     3901 ggcgatgtcg tcatatcggt ggagagggcc gccgaggagg cgagggatgc gcacatgagc
     3961 acgaacgagc gcatcaggca gctcctggtt cacgggatct gccaccttgc ggggttcaac
     4021 cacgaggacg tcaccgagga agaggccctc gccatggaag aggccgagcg catggtcctc
     4081 catgccatat ctggcggcaa gaacgagtga ggcgcccagg gtttccggag gaggcgctgg
     4141 ggatagcaaa ggaccccgtg gcgcttttcg gcgagggctg gagagttcgg aaggcctggg
     4201 tttggcacat gttctccatc gggctccgcg acggatccgt cgctaaactg cacgttcaga
     4261 aggcctgggt ttcgcgacac gttctccctc gaggaccgtc cccctagggg gaggacgacc
     4321 gggaagccca aacggcctcg gtggtggatc caagagccgg tgcgcggttg ctaagcccca
     4381 tctcttaagc ccagcttctt gcctcttggg tatgacgtgt gagcgcgatg cccggggggc
     4441 cgccatgggg acccgggaac gcgggccgtg gggagagaag acctgatcag gagacctcag
     4501 gctccccatg tcatccccag ttacccctgt gcgctcggtg gggagagagc atgtgaccag
     4561 gagatcccag agaggttcga agggcctcca cgctcctgtc cgtgtggccg gggggcgaac
     4621 cgggagacgt cggcgccgag cttgggttcg gcggtgaggc gggtctctgg ggcgcgccca
     4681 tgaaggtccc accgttcgca tccggggcaa atcaataagc aaatcgataa gatgggagct
     4741 gcatggcccg gggaaaaagt gcttgccatg ccatataccc tctgctataa ggctcgtcta
     4801 cggggcgtag cgcagcctgg caagcgcacc tgccttggga gcagggggtc ggaggttcaa
     4861 atcctctcgc cccgaccagg caggggcgac gtgggcgcct gtagctcagt cggatagagc
     4921 aacggacttc taatccgtag gtcagaggtt cgaatcctct caggcgtgcc agagaacata
     4981 tggtgagtgt agctcagctg gtagagcgca ggattgtggc tcctgtggcc gagggttcga
     5041 tccccttcac tcaccccaga tacgaacgac ggatgcgccc gtagctcagc cggatagagc
     5101 gtcggacttc gaatccgcag gtcgggcgtt cgagtcgccc cgggcgtgcc acgctatacc
     5161 agaaacggga catgggccat tagctcaaca ggtagagcaa ctgactctta atcagtaggt
     5221 cccaggttcg agccctggat ggcccaccaa cacatccgag gagtgaaacc tcctcggatg
     5281 tttcctttca aaggccttgc gcgttgtccc tcaccgtctg cccgccctct cgacgagcgt
     5341 ctcttccagg caggcattca agcgcggtcg gggttcgtgt ttgcgggcat gcgccccttt
     5401 tccctccggc attcaagaac gcaggccccc ggcggtgtga ccctttcggg ccactcgccc
     5461 ggttcatcgc tggggaagac aagggcatga cctccacctg aagcacggtc tgggcggcgc
     5521 ctggtgacag gtctgtcgcg gggcctttcg aaccggccgg aggcctcggt acaggctgga
     5581 gaacccttcc cgatcccggt gacaggggat gccttgtaga cgggccgccg cgcgcaagag
     5641 cggcggttga aaggccgcgc ccaagggggc gagaagatgc cggtgtgacc accgtcgcca
     5701 cgcgtgcacg ggtggcttcc gccccgcgga ctacgacccc gcagtggacc tggaggaaga
     5761 cccgcgcata ccccgggtcg caagggaggc cctccctcgt cgcgcgccga gcgctgtaca
     5821 gacagccaag agcattccgg caatgaggtg ccaagacggc gtgttcgcgc cgggcatgcc
     5881 ctgtcggtgt ctgtggctgt cacgaagcat ccgaggtgct gatctcgagg cgactcgtca
     5941 aacgaagcgg cctccccgtc cacgccgagt cctcccgggt cgacgatgac gccgttggct
     6001 gcgccgtctg agtctccgta atccggatca ccgtccttga gcgtgagcgt gacgctgagc
     6061 cggtcttcgc tgagcacggc gtgggggtac tccctccagc cgtttctctg gtcgaagaag
     6121 taccacttcg aacccaccgg tgccttcctg gggaggtaga cgatcacctc ggcaaggtcg
     6181 ccgggccgat cgagttccat cttgaaatcc acgatcccga tggggaggta tgccggcttg
     6241 gtctcgaagc cttcaccccg cgcggtgtcg agcgccttga acacctttat ccccctgcag
     6301 ttcgtggatg ccttgaggct caacggcatc ccgccttcgg tggtgaggat cgtgagcatc
     6361 ccttcctgga cgaggtccga gaccccgttc ctgtcgaggt ccaccctggc gcctgccggg
     6421 ggttcctggt catccggcac gccgtttcca tcgagatcct cagtgtcgtc ccgtgcggtt
     6481 ataaacagag tgggctcgga ccactcggtg tatgtcccgg aaccgtcgtg gtaccttacc
     6541 ctgcaatagt attcggtgcc gccctcgagc acgaggtcgg gcacctggaa tccatccgtc
     6601 ggcgaggcga cggtggcggc caggacgaga tcacccgagg cgctgctgat ctcccactgg
     6661 ccgtaccagt ggtcaccggg aggcggggtg gcctcgagca ggggcgtgag ctcgacgtcg
     6721 gcctcgttgt cgagggggga gacgaccacc ggcctcggca gggtctcagg cggtgactgc
     6781 gtgtcgtctg cgtcgccggg gtcggggtcc gcggactggt tggggcttgt atctccgctc
     6841 gaggcgacgc tgctcgaagg gttggaggtc gcggcgccgg cggggttggt tgcctgggtc
     6901 gagccggtgg aaccggacgg ctgtgacggg ctcgtc
//
LOCUS       DAOSCR010000055         6893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7069_length_6893_cov_3.416642, whole genome shotgun sequence.
ACCESSION   DAOSCR010000055 DAOSCR010000000
VERSION     DAOSCR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7069_length_6893_cov_3.416642"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            711..2714
                     /locus_tag="PLT69_02510"
     CDS             711..2714
                     /locus_tag="PLT69_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13346.1"
                     /translation="MNTASHGRTALLASASILSLALAAGSAALYLAIGMRGASRREAD
                     RETAYAEAFMGVSPDLFVVLDGDGYVVAMNQAMRGILNMSETMGATPSLPRVLLPAFI
                     PECHRDRFMENLREAARTDETVSFECGVVNARGQAYHVTWHARRLHDGPQPGCMYVLA
                     GIDITGHKKTQEELASSERRYRLYAENVRDVLWVLDRDLNYVYISPSVYLLRGYTPEE
                     AMRMPIDQVFTKDSRRKIIKLTSSVKSLLAKGERIDPQNTLKMELEQFCKDGSTKWTE
                     VIANILLDDEGNHVGFLGITRDITERKLAQESLRLSEERYRTIFETTGNAMAIVRHDD
                     VISLANGEFERLSGYFRHDIEGKMSWTDFIDVQDRDRVAKSTPSGANDQASTRCNYDF
                     RFVDRWGRTHMVTASSTLIPGTSMRVVSLLDITDMIEAQERLKASREQLRKLHIHTQE
                     LRERERANVAREIHDELGQVLTALKMDLSYLARRLHPDLEHLHAKISSMSRSIDASID
                     SVRRIIMELRPGLLDHLGLVPAIEWQIGEFERRTGIACTLSVSHTDIDIDREVATTVF
                     RILQEALTNVARHAEATEVSVSMSIDQNTLILTINDNGRGITQNEIEDPTSFGLMGMK
                     ERALFCSGAFEISAGRHGKGTRVTLRVPLSKTGKDHDTGVGGG"
     gene            2689..3357
                     /locus_tag="PLT69_02515"
     CDS             2689..3357
                     /locus_tag="PLT69_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ13347.1"
                     /translation="MIRVLVADDHPIVREGVKQILSETEDIIVSDEASTGKEALDLVL
                     KNAYDVVILDISMPGRDGLETLKELKSARPKTPVLILSIYPEEHYAVRVLRAGAAGYV
                     AKASAPDELIAAIRKVASGGRYLSPKAAERLTYELDRDFSKPLHEQLSDREHQVMRLM
                     ASGKSIKEIAAELCLSIKTVSTYRGRVFEKLGVSTNAALVLYALKNGLIDEGQIPPAD
                     TVDR"
     gene            3708..4469
                     /locus_tag="PLT69_02520"
     CDS             3708..4469
                     /locus_tag="PLT69_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13348.1"
                     /translation="MGTLDLSRLPADAGSVFRPFAEEALRRFPDRIHALCVIGSCLTG
                     DFVSSVSDINSVFVMASTDVDDLDVLAGIRAAHAKKRISPPLVMTVEYIERSLDAFPV
                     EFLDMKLFHATIAGTDPFSGLAVDRGALRLQCERDLKAKLVNLSRGYLSCAGSTRDLS
                     ALLQEALPGYFPLLRAMLFLVRAEEGIPGPKKEVLRAVEEAFGLSLDTLRELMEIRVA
                     GFRLRDWSRLKTLFQELHGITHDLSTTIDRMCTRE"
     gene            4480..5220
                     /locus_tag="PLT69_02525"
     CDS             4480..5220
                     /locus_tag="PLT69_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOJ13349.1"
                     /translation="MSARPLDLTRQRRSIPRMRVPAALCLILACTLWTSSARSQENFY
                     VQDTAGVINAPTRTAVTALLSELEQKTGAQFVVLTVKTTGGVPIEQFSLEKAQSMRLG
                     RKDVDDGLLLLIAVEDRKYRFETGYGLEQVLPDSLLGSVGRRYLVPSFKRGDYSQGIL
                     DAARVISATIARSRGVKITGMEEPTQAKKRAGFPVAPLLTFFAIAVILAALSGPHRRG
                     GIADALVIGSILRGAGHRGTGGGASGGW"
     gene            5690..6253
                     /locus_tag="PLT69_02530"
     CDS             5690..6253
                     /locus_tag="PLT69_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ13350.1"
                     /translation="MGKTARVIVGVAFVVILAVVFAVSGFNKARRMNEAVNAQWAQVE
                     NQLKRRYDLIPNLVETVKGYAAQEKSVFLGIAEARKAYFQAQDVAGRARAAGVLETAL
                     SRLLLLQGRYPELKSNENFLKLQDSLEGTENRISVERKRYNDLVRDLNSYVKTFPGMV
                     FARWAGVSEAQYFDIPEAEKETPKVRF"
     gene            complement(6250..>6893)
                     /locus_tag="PLT69_02535"
     CDS             complement(6250..>6893)
                     /locus_tag="PLT69_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ13351.1"
                     /translation="PRGIGDRALHVIREIAGSEGLSLMEAMQAARARKTLKGAARKGL
                     EAFCSLMEDLQRNIHGMDIAGMLLYLIDKTGYRDYLLQETDGVDRVLNVQELVSSAQG
                     QNDLVSYLQEKALLTTADAAQGTKAVSLMTLHMSKGLEFDHVFIAGCEEGLLPHALSC
                     EDSSQVEEERRLLYVGMTRARRRLVLSWARVRGIYGRQTYQVPSGFLQEIEAG"
BASE COUNT         1360 a         2181 c         2101 g         1251 t
ORIGIN      
        1 ctgaacaggg cccgcatctc gtgaaggccc aaccgctcgc acgggagact gccgtcacct
       61 ggagcgacac ggggaatcct ggaatccggg aacccgtcga aaccgccctg ttcggctcct
      121 tccaccctgc ggaacgtctc cttgcgtgcc cagcgttgtc tccatggaga gaggccgccc
      181 gccattgccg agcgcaaccg ggggcgacca ggggcccctt ggcctttcgt gcctcttgtg
      241 cccgtcattg agcctgcatc gctgcgcaag ggcaaggatc cactctccat ctcagaaacg
      301 agacccctgc cgggtgggtg tccagacagg cggcccccgt gtctcatcac ctttcggatg
      361 catgcctctc tgccgaaacg tgagccgccc ggtctcggat tctctgggtg acgtcatgag
      421 aaaggcacgg gaaaccggct tgtacatcac gaaccatgga catataatcc ctgccggcta
      481 ccggttcaac gcgtctcgat gcacggaagc ggtgcagggc gccctgcatg ggccgtgaga
      541 ccacgacgcc ccgagcagag cgaggggccc ctggggagac catgggcgac aggctgtgtt
      601 ttcaagggaa gagacgctcc accttccggc acggactccc aacggcaggg ttcgtgttcg
      661 cctcggtgtg ggcatcgacc ctcctgtgcg cattcctgat tacgtggatt gtgaacacgg
      721 cttctcacgg ccggacggcc ctccttgcat cggcgtcgat cctatccctc gccctggcgg
      781 cgggatccgc ggccctgtac ctggcgatcg gcatgcgggg ggcgtcgagg cgcgaggctg
      841 accgcgagac cgcctatgcg gaggcgttca tgggcgtctc ccctgacctc ttcgtcgtcc
      901 tggacggcga tggatacgtg gtggcaatga accaggccat gcgcggcatc ctgaacatga
      961 gcgaaaccat gggagcgaca ccctcccttc cacgtgttct gctgcccgcc ttcatccccg
     1021 agtgccatcg cgaccgtttc atggaaaacc tgcgcgaggc cgcgcgcacg gacgagacgg
     1081 tttcgttcga atgcggcgtc gtcaacgccc gcgggcaggc atatcacgtg acctggcatg
     1141 cccgaaggct gcacgacggt ccccagcctg gctgcatgta cgtcctggcc gggatagaca
     1201 ttaccggcca caagaagaca caggaggagc tggcctcgag cgagcgtcgc tacaggctgt
     1261 acgcggaaaa cgttcgtgac gtcctgtggg tcctggacag ggacctgaac tacgtgtaca
     1321 tcagcccttc cgtgtacctg ctcaggggct acacgccgga agaggccatg cggatgccca
     1381 tagaccaggt cttcacgaag gattcgcgca ggaagatcat caagctgacg agctcagtga
     1441 agtctctgct cgccaagggc gagaggatcg accctcagaa caccctcaag atggagctcg
     1501 agcaattctg caaggacggc agtacgaaat ggaccgaggt cattgcgaac atcctcctcg
     1561 acgacgaggg caaccacgtg gggttcctgg gcatcaccag ggatatcacg gagaggaaac
     1621 tcgcccagga gtccctgcga ctcagcgagg aacgctacag gaccatcttc gagaccacag
     1681 gcaacgccat ggccatcgtg cgtcatgacg atgtcatttc cctcgcgaac ggcgagttcg
     1741 agaggctttc cggttacttc aggcacgata tcgagggaaa gatgtcgtgg acggacttca
     1801 tagacgtcca ggacagggac cgggtggcga agagcacccc gtcgggcgca aacgaccagg
     1861 cgagcaccag gtgcaactac gacttccgct tcgtcgaccg ctggggtcgg acgcacatgg
     1921 tgaccgccag ttcgacgctc atacccggta cctccatgag ggtcgtctcg ctcctggaca
     1981 tcaccgacat gatcgaggcg caggagaggc tcaaggcctc acgggaacag ctccggaaac
     2041 tccacataca cacgcaggag ctcagggagc gcgaacgcgc gaacgtggcc agggaaatcc
     2101 acgacgagct tggccaggtg ctcactgcgc tgaagatgga cctgtcctac ctcgccaggc
     2161 gcctgcatcc ggacctggag cacctccacg cgaaaatctc ctccatgagc agatccattg
     2221 acgcgagcat cgactcggtg aggaggatca tcatggagct caggcccggc cttctcgacc
     2281 acctcgggtt ggtcccggcg atcgagtggc agataggcga gttcgagagg aggaccggga
     2341 tcgcgtgcac cctgagcgta tcgcacacgg acatagacat tgacagggag gtggccacca
     2401 ccgtgttccg catcctccag gaggcgctca ccaatgtcgc ccgacacgcg gaagcgaccg
     2461 aggtgagcgt ctccatgagc attgaccaga acaccctcat actcaccata aacgacaacg
     2521 gcagggggat aacgcaaaac gagattgaag atcccacctc ctttggtcta atggggatga
     2581 aggaacgcgc actgttctgc agcggtgcgt tcgagatatc ggccgggcgt cacgggaaag
     2641 gcacccgggt gacgctcagg gttcctctct caaagacagg caaggatcat gatacgggtg
     2701 ttggtggcgg atgatcaccc catcgtgcgc gagggggtca agcagatcct ttccgagacc
     2761 gaggacatca ttgtctcgga cgaggcatcg acgggcaagg aagcgctcga tctcgtgctc
     2821 aagaacgctt acgacgtcgt gatcctggac atctccatgc ccgggcgcga cggtctcgag
     2881 acgctgaagg agctgaaatc cgcaaggccg aaaacaccgg tgctcatcct gagcatttac
     2941 ccggaagaac actacgctgt gcgggtcctg agggcagggg cggccgggta cgtcgccaag
     3001 gcgagcgcac ccgacgagct catcgcggcg atcaggaagg tggcatccgg cggcaggtac
     3061 ctgagcccca aggcggcgga acgcctcacc tacgaactcg acagggactt ctcgaagccg
     3121 ctgcacgaac agctctcgga ccgcgagcac caggtcatgc gcttgatggc ttcaggcaag
     3181 tccatcaagg agatagcggc agaactgtgc ctgtccataa agaccgtgag cacctaccgg
     3241 ggcagggtct tcgagaagct gggtgtctcc accaatgcgg ctctcgtcct ctatgccctg
     3301 aagaacggcc tcattgacga gggacagatc ccccctgccg ataccgtgga caggtgatcg
     3361 cgacccacgg cagagggcat ggccgggaaa agagcgcctt ccgccggcaa gctgtaggac
     3421 aattcctaca tgcatgccgg aaattttctc attcaacatt cagcactttt acccttcacg
     3481 gcgcttgaca agtatgtcat tatgacaatc acaacaagtg ttgttctgac ctcctttacg
     3541 ccagaagagg ggacaaatcc tctcgtgctt tgtccttacc caaacttctt ctggctcttt
     3601 tttttgcccg cgtttcgtgt cctacagtgt gcctgtacgc atccacgcga aaacccttgg
     3661 acagaaaacg cccacatggt accatgcatg gcctgggggt tcgatcgatg ggaacgctcg
     3721 acctgagtag gttgccggcc gatgcagggt ctgtcttcag gcctttcgcc gaggaggcgc
     3781 tccggagatt cccggacagg atccacgccc tctgcgtgat cggcagctgt ctgacgggcg
     3841 atttcgtcag ctctgtctcg gacatcaact cggttttcgt catggcatcg acggatgtcg
     3901 atgacctcga cgtcttggcc gggatacgcg ccgcgcacgc aaagaagagg atcagcccgc
     3961 ctctcgtgat gaccgtcgag tacatcgaga ggtcgctgga tgcgtttcct gtggaattcc
     4021 tcgacatgaa gctcttccac gcgaccatcg cgggcaccga ccctttctca gggctcgccg
     4081 tcgacagagg cgccttgagg ctccaatgcg agcgcgacct caaggccaag ctcgtgaacc
     4141 tctcgagggg atatctctcg tgcgcgggat caaccaggga tctctcggcc ctcctccagg
     4201 aggccttgcc cgggtacttc cccctgctca gggccatgct cttcctcgtc agagccgagg
     4261 aggggatccc ggggcccaaa aaagaggtgc tacgtgcggt cgaggaggcg ttcggcctct
     4321 ctctcgacac gttgcgggag ctcatggaga tcagggtcgc cggctttcgc ctgcgcgact
     4381 ggtcgaggct gaagacgttg ttccaggaac tccacggtat cacccatgac ctgtcgacca
     4441 cgatcgatcg catgtgcacc cgggagtgaa cgtggccgca tgagcgcccg ccctctcgat
     4501 ctcacccgtc aaaggcgctc gatcccccgc atgcgagtcc ctgctgctct ctgcctgatc
     4561 ctggcgtgta cgctctggac ctcgagcgca cgttctcaag agaacttcta cgtgcaggac
     4621 acggcgggcg tgataaacgc ccctacccgc acagcggtga cggcccttct ctcggagctc
     4681 gagcaaaaga ccggggccca gttcgtggtc ctcacggtga agaccacggg gggcgtgccc
     4741 atcgagcagt tttcgctcga gaaggcacag tccatgcgcc ttggccgcaa ggacgtggac
     4801 gacggccttc tcctgctcat agcggtcgag gaccggaaat accgtttcga gaccggctac
     4861 gggctggaac aggtccttcc tgacagcctt ctcggaagcg tcggcaggag atacctggtc
     4921 ccctcgttca agagggggga ctattcgcaa ggcatcctgg atgcagcaag ggtcatctcc
     4981 gccacgatcg cccgatcccg gggggtgaag atcaccggca tggaagagcc cacgcaggca
     5041 aagaaacgag cgggcttccc cgtcgccccc ctgctcacct tcttcgccat cgccgtcatc
     5101 ctcgccgccc tgtcgggacc gcaccgcagg gggggcatcg ccgacgcgct cgtcatcggg
     5161 tccatcctca gaggcgcagg ccacaggggc acaggcggcg gggcgtctgg aggctggtga
     5221 gagccgcctt cgagggcggc cgttgttgtg gggctgcccc ccgggcccgg gcctctcttc
     5281 tcatacttct ccctcgaata ccctccccca tgaaaacgaa tgagcgtgac ctccttcctg
     5341 atgacgattt ccggcctcgt tgacgatgac gatctccagc gtccgggatt gctcatacgc
     5401 cgggcagggc aagaggaacg acgacagccc aagcccaccg tctggcaccc agggcgagac
     5461 tgtcgccgcc cagggggctt gccgcttttc ggcctcccct cttcaccttg tccatgtgtc
     5521 gttcggcttg gtgtcaagcg ttactgccga ttgccgcttt tctgccaatc accgccatct
     5581 gtcgtacggc ctgatcgaag agccgcgatg cctcttgatg cttgtgcgtg agcctcggca
     5641 tcgggtatag aaccggttgc aacccatctc acaagcacgg gggggtacca tggggaaaac
     5701 ggcgcgggtg atcgtgggcg tggcgttcgt cgtcatcctc gcagtggtgt tcgccgtatc
     5761 gggattcaac aaggcgcggc gcatgaacga ggcggtgaac gcccagtggg cccaggttga
     5821 aaaccagctc aagaggcgct acgacctcat ccccaacctc gtggagacgg tcaagggata
     5881 cgcggcccag gaaaagagcg tttttctggg catagcggag gccaggaagg cctacttcca
     5941 ggcacaagac gtggccggga gggcaagggc cgccggcgtg ctcgaaaccg ccctctcgag
     6001 gctcctcctg ctgcagggaa gataccccga gctcaagtcg aacgagaact tcctgaaact
     6061 ccaggactct ctggagggca ccgagaaccg catctcggtt gaacgcaaga ggtacaacga
     6121 cctcgtgcgg gacctcaact cctacgtcaa gaccttcccg ggcatggtct tcgcaaggtg
     6181 ggccggggta agcgaggccc aatacttcga tataccggag gcggagaagg agacgcccaa
     6241 ggtcaggttc taacctgcct cgatctcctg caggaacccc gagggcacct ggtaggtctg
     6301 cctcccgtag atcccacgga cgcgggccca agagagcacc aggcgcctgc gggcccgggt
     6361 catgcccaca tacaggaggc gtcgttcctc ctctacctgg gaggagtcct cgcatgacag
     6421 cgcgtggggc aggagcccct cctcgcaacc cgcgatgaag acgtggtcga actcgagccc
     6481 cttcgacatg tgaagcgtca tgaggctcac ggccttcgta ccctgggccg cgtcagcagt
     6541 cgtgaggagg gccttttcct ggaggtatga gacgaggtcg ttctgcccct gcgcgctcga
     6601 gacgagctcc tggacgttga gcacccggtc cactccgtca gtttcctgga ggaggtaatc
     6661 ccggtaccct gtcttgtcta tcaggtacaa cagcatgccg gctatgtcca tgccgtggat
     6721 gttccgctga aggtcctcca tcagggagca gaaggcctcc agcccttttc tggcggctcc
     6781 cttgagcgtc ttccttgccc gggccgcttg catggcctcc atgaggctca ggccttccga
     6841 cccagcgatc tcgcggatca cgtgcaacgc cctgtctcct atgcccctgg gcg
//
LOCUS       DAOSCR010000056         6830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7146_length_6830_cov_3.502288, whole genome shotgun sequence.
ACCESSION   DAOSCR010000056 DAOSCR010000000
VERSION     DAOSCR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7146_length_6830_cov_3.502288"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLT69_02540"
     CDS             complement(<1..492)
                     /locus_tag="PLT69_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOJ13352.1"
                     /translation="MRLRYRKGVDIVFYEVFKEEREALKRHLPGNTKAAFFPLTIQES
                     GHTTLPGGILCIRTQSVIPRAWERDIQAILTRSTGYDHVLSFLEASHRDIPAGYLPHY
                     CSRAVAEHALMMWMALMRRLPAQIENLTRFDRDGLTGWECLGKTVAVVGVGSIGSEVC
                     RLAG"
     gene            538..1845
                     /locus_tag="PLT69_02545"
     CDS             538..1845
                     /locus_tag="PLT69_02545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ13353.1"
                     /translation="MSRSGPLKFATCATVTMHVGHQRRPGAWVRDMRKVMTFLVPGGV
                     LALASLLLVRLDAMEGCLDEVSRLYPVLATALGVAYGLRFDRSSLVLGALCPAMALWS
                     LSFFGSIHDPTDMHWRVAYVSCSVLLPVNLCVLCLLKERGTLTARGIARLLAILAQPL
                     VVGAVAGRDPSRFVNVLERGVIPWGFAEALPLPQVSALAGAVCLILTAVLFVVRGGAR
                     EAGFFWAQLASIAALASGPDREVAAFHLGSASLILLVSMVEISHAMAFKDELTGLPGR
                     RALKEELLKLGSEYCLAMVDIDHFKKFNDTYGHDAGDQVLKMVASRLGHVGGGGRAFR
                     YGGEEFTIVFSGRNAQDALEHLEELRKDVASFPFVIRSALRQIRSPDRRGVRSHGRKN
                     VTITISVGAAWPGGSASKPEEVLEAADKALYRAKKRGRNRVES"
     gene            2604..2933
                     /locus_tag="PLT69_02550"
     CDS             2604..2933
                     /locus_tag="PLT69_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13354.1"
                     /translation="MAGDVNFKDTRAGRRTSDARCARAAQAIMEILKYQCQGFILPPP
                     EVRHLLGLDPEDRAWHLNERVALLKKELARRFVAMDVSPIVGATIQLYRVPPVNGAPY
                     PSRYLRQ"
     gene            3252..4271
                     /locus_tag="PLT69_02555"
     CDS             3252..4271
                     /locus_tag="PLT69_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ13355.1"
                     /translation="MGITYVPAEATGRAAIDALDTLLGMLDVPFSRGSRVGVKLHWGE
                     RGNKSYLEPAYARGIVRWLKDLGMDPFVFDTTVLYSGGRRNAADSLETAASHGFTRDF
                     LGCDVVIADGMDGRNVVDIPAGYKHFKTVQVASIVHEADGFVIFSHFKGHLAAGFGGA
                     VKNVSMGFASRAQKQRMHADVKPVLNQKRCTKCGVCASVCPTGAASMQGDGYPSYDLE
                     VCIGCAQCIAQCPEVALKILWGGDHEAFQEKLVETAKAVWDVIGRRTTVINALVKISA
                     ECDCLPGKVEIISEDKGFVAGTHPVEVDADCVRRVGEDVITKAHSYLPWQRQFSYARE
                     IGFVY"
     gene            4261..4632
                     /locus_tag="PLT69_02560"
     CDS             4261..4632
                     /locus_tag="PLT69_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13356.1"
                     /translation="MCIDPRDPRKHRAAAGGKRALLGVLASCALCGCVITDDGCCTVV
                     EVLYNEGGLQVHVTLGRPVDLPLDEDDATYIMVTCPCPCRGELTRVGDYWQYPGRDTG
                     EGCLIRIHVLTGEVECFPQDG"
     gene            5116..6042
                     /locus_tag="PLT69_02565"
     CDS             5116..6042
                     /locus_tag="PLT69_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ13357.1"
                     /translation="MSLRKLSDIFSLEEVLTLDMAVLFALAALAAGGPAGTKGILATN
                     CVIVACIVSAAVLCRNSGKRALTVFRDWYVLVFLITIYLENRTLIPLVNPREYDALVM
                     AADRLVFLGEDPTVLLEKVMHPLLTEFLQLVYASFYLLPLTLCMALYASRTTRNEFHV
                     AASTILMGFYLSYLGYYLMPVAGPRFFMEGLHTKELEGLWTFEFVREALARAEGRMLD
                     CMPSGHALVSVLTTLLAYRYVRSFAPWSLVWTVLLLFSTVYLRYHYVLDLAVGSMLGI
                     LVFTIGPRLAIAYQEGSGSRRCVAGGLEIREG"
     gene            complement(6330..>6830)
                     /locus_tag="PLT69_02570"
     CDS             complement(6330..>6830)
                     /locus_tag="PLT69_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13358.1"
                     /translation="DMDRRIKEIQRIFNIAWEGNWGHLPLTDAQVKMIASDLKLIAKE
                     ELAIFAEKDGETVGFIVSLPDVNPALRLLNGRLYPWRIVRFLLEARKTKRIRTIIMGV
                     LPEHRGKHIDDIFYLMTIEDGIRLGYTESDCSLIVETNTRMINALKPLAPSPYKTYRI
                     YEKRLD"
BASE COUNT         1146 a         2076 c         2322 g         1286 t
ORIGIN      
        1 gccggcgagg cggcacactt ccgaaccgat ggatcccacg cccaccaccg cgaccgtctt
       61 gccgaggcac tcccacccgg tgagaccgtc cctgtcgaag cgggtgaggt tctcgatctg
      121 cgcagggagc ctgcgcatga gggccatcca catcatgagg gcgtgctccg ccacggcccg
      181 gctgcagtag tgggggaggt acccggcagg gatgtccctg tgcgatgcct cgagaaagga
      241 gagcacgtgg tcgtaaccgg tgctcctggt gaggatcgcc tgtatatccc tctcccacgc
      301 acgggggatg accgactgcg tccgtatgca caggattccc ccgggaagcg ttgtgtggcc
      361 tgactcctgg atggtgaggg ggaaaaacgc cgccttcgtg ttcccgggca gatgccgctt
      421 gagggcctcg cgctcctcct tgaagacctc gtagaagacg atgtccaccc ccttcctgta
      481 cctcagccgc atgccccatg gcaacaggcg ctcgcggagg aaggaccctt gaagggggtg
      541 agtcggtccg ggccgctcaa gttcgccact tgtgctaccg taacgatgca cgtgggccac
      601 cagaggcggc ccggcgcatg ggtgagggac atgaggaagg tcatgacatt cttggttcca
      661 ggcggcgtgc tcgcgctcgc atccctcctc ctcgtcaggc tcgacgcgat ggagggctgc
      721 ctcgacgagg tctcccggct ctaccctgtc ctcgccacgg ccctcggcgt cgcctacggg
      781 ctcaggttcg accgctcctc gctcgtcctc ggtgcgctgt gtcccgcaat ggcgctctgg
      841 tcgctctctt tcttcgggtc catccacgat cccacggaca tgcactggcg cgttgcgtat
      901 gtgtcgtgca gcgtgctttt gcccgtgaac ctgtgcgtgc tctgcctgct caaggagagg
      961 ggaacgctga ccgcaagggg gatcgcgagg ctcctcgcga tccttgcgca gcccctcgtc
     1021 gtgggggcgg tcgcgggtag ggacccctcc aggttcgtga acgtgctcga gcgcggggtg
     1081 atcccgtggg ggttcgcgga agccctccct ctcccccagg tatcggcgct tgcgggcgcg
     1141 gtgtgcctga tcttgaccgc ggtcctcttc gtggtcaggg gcggggccag ggaggcgggg
     1201 ttcttctggg cgcaactcgc gagcatcgcg gccttggcct cggggccgga ccgagaggtc
     1261 gcggcctttc acctggggag cgcatcgctg atactccttg tctccatggt ggagatctcc
     1321 cacgccatgg cgttcaagga cgagctcacc gggctgcccg ggagaagggc gctcaaggaa
     1381 gagctcctga agctcggctc cgagtactgc cttgccatgg tggacatcga ccacttcaag
     1441 aagttcaacg atacctacgg tcacgacgcc ggagaccagg tgctgaagat ggtcgcctcg
     1501 cggctgggac atgtgggtgg tgggggcagg gcgttccgtt acggcgggga ggagttcacg
     1561 atcgtgtttt ccgggaggaa tgcccaggat gcactcgagc acctggagga gttgaggaag
     1621 gacgtggcgt ctttcccgtt cgtgatccgg tcggcgctga ggcagatcag gagcccggac
     1681 cgaaggggtg tccgttcgca cgggcgcaag aacgtcacga tcaccataag cgtgggcgcg
     1741 gcctggccgg ggggctctgc ctcgaagccc gaagaggtgc tcgaggccgc ggacaaggcc
     1801 ttgtacaggg cgaagaagag gggaaggaac agggtggagt cttgatcccg aggcctcggg
     1861 ccggccttca ggggtgcacg tttcggccgt ctttcccgtc aggtatcctg gaaaaggtac
     1921 tcctcgacct ccacgcgcct tgaaagacgt acccgtcagt catccaggca gggaagaggt
     1981 ctctccgaac tcctggcaac aacgcgctca ggtcctgcct tgccgggccg tcgtccttgc
     2041 ccgggaaggg cgcgaccggg gatcggggcg aagaaccggc gcggctcggc cgggccggcg
     2101 caaggtgcgc ccagccgtgg agagccgcct gattcctcgg ggggtgaccg ggggtgatcc
     2161 tgtaggtacc caagaaaaac cgttcctggc ggtgacctgg ggaacatgga ggtggtcggg
     2221 gttccgccgg tttccccctt gcaggacggt cgacgaaaga cacccgatag agagacgggc
     2281 tcaggtttca tcttcctgaa gcggacaagc gttgttcaac ggcactcagc cctgccgacc
     2341 ggtgaggcat cgtaagcggg acctggggcg atccggtgcc cccctgtgtg cctggcgccg
     2401 agagcaggcc gagggcaaga aaacgacaag agtgaggaaa aacaattccc tcttgtgggc
     2461 cggctcgctt gtggctttat aacatactgg aatgcctaat aaaaactata aatgaacagc
     2521 cgttagcctg gcacgggtat tgctgtcatg aggggtagat tgtttcatat tatagcaata
     2581 aaagtggcgt gggaggtgac ggtatggcag gtgacgtgaa cttcaaggac acgagggccg
     2641 gcaggaggac ctcggatgcg aggtgtgcaa gggcggccca ggccatcatg gagatcctca
     2701 agtaccagtg ccaggggttc atcctcccac ccccggaggt caggcacctc ctgggcctcg
     2761 atcccgaaga cagggcatgg cacctgaacg agcgcgtcgc attgctcaag aaggagctcg
     2821 caaggcgctt cgtggccatg gacgtgagtc ccatcgtggg tgccacgatc cagctctacc
     2881 gcgtgccccc cgtgaacggg gcgccgtatc cttcgcgtta cctgaggcag tgacagcctg
     2941 cttctcgggc ccaggcgccc gtatctgcca cgggccctgc cttcctgccc gaggtctctt
     3001 ccacagtcac atcgccctcg gggttcgccc ttgcgcaggc caacgggcga gccgacctcg
     3061 ggcatcccca ccccgcaccc tgccgcgaac gggcacgcgg gtgttcccga cacttcatgc
     3121 ctgtcccgtg cagcccgcac cctgccgtga acggccacgc gtcctgatgc aagcggggag
     3181 gtgagatgtt gacatggggc gggaatcggg cacattggat atgacgcaca acacagacgc
     3241 acgaggtgat catgggtata acctacgtac ctgcagaggc taccggccgg gcagcgatcg
     3301 atgccctgga taccctgcta gggatgctcg atgtcccctt ttcgcgcggg tccagggtgg
     3361 gcgtgaagct ccactggggg gaaaggggca acaagagcta tctcgagccc gcctacgcgc
     3421 gcgggatcgt gcggtggctc aaagacctcg gcatggaccc tttcgtgttc gataccaccg
     3481 tgctctactc agggggcagg aggaacgcgg ccgactccct cgagacagcc gcttcccacg
     3541 gattcacgag ggatttcctg ggatgcgacg tggtcatagc cgacggcatg gacggccgga
     3601 acgtggtgga catccctgcc ggctacaagc acttcaagac ggtgcaggtg gcatccatcg
     3661 tccatgaggc cgacggcttc gtgatcttct cccacttcaa ggggcacctc gcagccggct
     3721 tcggcggcgc cgtgaagaac gtgtccatgg ggttcgcgtc ccgcgcccag aagcagcgca
     3781 tgcacgcgga cgtgaagccc gtgctgaacc agaagaggtg cacgaagtgc ggggtgtgcg
     3841 cgagcgtgtg ccccacgggg gccgcgtcca tgcaaggtga cggctacccg agctatgacc
     3901 tcgaggtgtg catcgggtgc gcccagtgca tcgcccagtg ccccgaggtg gcgctgaaga
     3961 tcctgtgggg aggcgatcac gaggccttcc aggagaagct cgtcgagacg gccaaggccg
     4021 tctgggacgt cataggccga aggacgacgg tcataaacgc gctcgtgaag ataagcgccg
     4081 agtgcgactg cctgccgggc aaggtcgaga tcatctccga agacaagggt ttcgtcgcag
     4141 gaacccaccc cgtggaggtc gacgccgact gcgtcaggcg cgtgggtgag gacgtgatca
     4201 cgaaggccca cagctacctg ccctggcaga ggcagttttc ctacgcgaga gagatagggt
     4261 ttgtgtattg atccccgaga cccaaggaag caccgcgcgg ctgcaggtgg gaaacgcgcc
     4321 cttttgggcg tgctcgcctc gtgtgcgctc tgcgggtgcg tgatcaccga cgacggttgc
     4381 tgcaccgtcg tcgaggtgct ctacaacgag ggcggtctcc aggtccacgt aaccctcggg
     4441 cgtcccgtcg atctccccct cgacgaggac gatgcgacct acatcatggt gacgtgcccc
     4501 tgcccctgca ggggagagct tacgagggtt ggggattact ggcagtaccc cgggcgtgat
     4561 acgggcgaag gctgcctcat ccgcatccac gtccttacgg gtgaggtcga gtgcttcccg
     4621 caggatgggt gagcagaagg cggcaggcgt cgagaccatg cgcttcccgt cgccgtcttt
     4681 tctctcccgg ctttttgggg atacgccgat cgaggcgtcg gggccgaacc cggtataagc
     4741 ggcggcaaca ggatggcggt ggaccgtgct cgtggggaat gcgggcgtga agaggcggga
     4801 cgtgtcgtgc gcggggatcg atggcgccac atccacggtt tgggctcgag acgagacggt
     4861 cttaggtctc cgggtaggcc gtgggcgccc atggggaagg gggagggtct tgagacgacc
     4921 cttctcgatg ccgtccggtc ggtcttcgca ctcgggcgta tccgcgttct gcgaccaagc
     4981 ggcgcgggtc cgggcatggc gatgggttga tgggagaggc cgcacgcttt tggggcgccg
     5041 tcgagccgct ccggcttgat ggaatcctgt gtggcggcta gaatccgtgt ctgcaacgcg
     5101 tgatcggggg gatgcatgtc tctgcgcaag ctgtccgaca tcttcagtct cgaagaggtt
     5161 ctcaccctgg acatggcggt gttgttcgcc ttggccgcgc tcgcggccgg aggtccagcc
     5221 gggacgaaag gcatcttggc gacgaactgc gtcatcgtgg catgcatcgt ttccgctgcc
     5281 gtgctctgca ggaacagcgg caagagggcg ctcacggtct tcagggactg gtacgttctc
     5341 gtcttcctga tcacgatcta cctggagaac aggacgctca tcccgctggt caacccacgc
     5401 gagtacgacg cgctggtcat ggccgcagac agactggtgt ttctcggaga agaccccacc
     5461 gtgctcctcg agaaggtgat gcatcccttg ctcaccgagt tcctgcagct cgtctacgcg
     5521 agcttctacc tcctcccgct caccctgtgc atggccctgt acgcatcgcg caccaccagg
     5581 aacgagttcc atgtcgccgc ctcgaccatc ctcatggggt tctacctgtc gtatctcggg
     5641 tactacctca tgcctgtggc gggccccagg ttcttcatgg aagggctcca cacgaaggag
     5701 ctggaagggc tctggacgtt cgagttcgtc agggaggccc tcgcccgggc cgaggggcgg
     5761 atgctggact gcatgccgag cggccacgca ctcgtctcgg tgctcacgac gttgctcgca
     5821 taccggtatg tgcgctcttt tgcgccgtgg tccctcgttt ggaccgtact gctcctcttc
     5881 tccaccgtct acctgcgtta ccactacgtc ctggacctcg ccgtcgggtc gatgctcggg
     5941 atcctggtct ttacgatagg gccccgcctg gcgatagcct accaggaggg ctcgggctca
     6001 agacgatgcg tcgcaggcgg gctggagatc cgtgagggtt gagggggctc gggccttagg
     6061 catcagggtc gctggctgcg ggcatggtgg tttttttcgc tgatggtgat cccaaaggct
     6121 tgagggggct cgaaaggccg gcgccaggga ccgatcgatc gactccgggt ggtcggcgaa
     6181 gccgcctgtc tcggccatgt gcccggcggg cgaggcgccg gatgacgtgc gtgggcgggg
     6241 gtggtgaaca ccatggcccc tctccccatc gaccccgcct ggggcggcat cgcagtctgg
     6301 ggtgagggtg gtgcgacgtg ggccgagggt cagtccaggc gcttctcgta gatgcggtac
     6361 gtcttgtacg gcgagggcgc caggggcttc agtgcgttga tcatgcgcgt gttcgtctcc
     6421 acgatgagcg agcagtcgga ctccgtgtac ccgagcctga tcccgtcctc gatggtcatg
     6481 aggtagaaga tgtcgtctat gtgtttgccc ctgtgctcgg gcagcacgcc catgatgatc
     6541 gtgcggatgc gcttcgtctt tctcgcctcg aggagaaacc tgacgatccg ccatgggtag
     6601 agcctgccgt taagcagcct gagcgcaggg ttcacgtcgg gcagcgacac gatgaacccg
     6661 accgtctcgc cgtccttctc ggcgaagatc gcgagctctt ccttggcgat cagcttcaga
     6721 tcggacgcga tcatcttcac ctgggcgtcg gtcaggggga ggtggcccca gttgccctcc
     6781 cacgcgatgt tgaagatgcg ctggatctcc tttatcctcc tgtccatgtc
//
LOCUS       DAOSCR010000057         6819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7163_length_6819_cov_3.463335, whole genome shotgun sequence.
ACCESSION   DAOSCR010000057 DAOSCR010000000
VERSION     DAOSCR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_6819_cov_3.463335"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="PLT69_02575"
     CDS             <1..1983
                     /locus_tag="PLT69_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13359.1"
                     /translation="LNDIWGVSEKDVFAVGDGGTILHFDGFDWVPMKSNTSKDLRSVW
                     GYSRESVYAVGENGVILRYDGDAWEALPRKTAHTLNGLWGSSERDLFAVGDMGTVLHF
                     DGSSWSTMDTGMLMDLHSVWGSGADDVYAVGEKGTMLHFDGSSWSLLHPMTTLDLMAV
                     WGSSADDVYVAGQGCVILHYGTSADRAMNTVAHARDARSTVGDPDMYAARTAFVPEDE
                     ALDGVTGETVDAQVVFQRRKNASKSLLEFLAILAEEKGLSRGGQALADAGSAPVGQGG
                     TDGRSTTMLAATESQKVTAAANARSETLVADLGVPAGAMDTNSSPSHVSVPDDGGAGT
                     RGAASMAPMDTMPETRVALGGKEPTPVAAGSRPSAEQPSKTPGLTPSTDLAGIAQETG
                     PGTPGVASTTEAGVGNNEVAEAARSQNDAISPAGKAEGLKSDELLARLDGSTSKPAET
                     QGSLTVEGDPGIGGNTSIEETGPQEVIMASAETGTAPAKAGDKDEPAPAGIDQGAFTH
                     HPSSRQTPSPEVRGEDRRTSARSLASIKPEGGDDHGTGGDMSGPGPAADSTGARTPTS
                     VASMAPAQDAVSGAQPSAKAGGTSLGSTATQWGMPTHARPYYPAESIYTHVLQWILAL
                     SLAGFFLFGLITTLVWREERVHRILLHLTSQVTPEK"
     gene            2249..4051
                     /gene="mutL"
                     /locus_tag="PLT69_02580"
     CDS             2249..4051
                     /gene="mutL"
                     /locus_tag="PLT69_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ13360.1"
                     /translation="MSRIRVLPPEVASMIAAGEVIERPASVVKELVENAIDAQAGSIV
                     VDIKGNGCDLVRVTDDGAGMTPEELDKAILRHATSKIGSAEDIFAIKTLGFRGEALPS
                     IAAVSRTTIASRPRSTDTGAALVIEAGVVTRRCLKGMPPGTTAEVADLFFNTPARRKF
                     LKTTATEQRSIIDVIARYALAYPKLAFQLGIDGRTVIDAPGNSSHEERARSLIGKSSA
                     DRMATFSRDTDMLRVFGLLGRIQDARKNRSGMYVFVNGRSVKDGMITAAIMGGYAGVL
                     VKGSYPVAVVFIEVDPREVDVNVHPTKAEVRFRNPAAVFGILSSVIKEAVSSSMHMNA
                     SSSPGEDAWLTGRGIPQAGRLAEAALPPHLHPYAGQRQGEDGPTRPRGRPVDAAETLP
                     LFGAERDGAGHAFSYAGLTILGSFRNAYLVLSDTDSLYILDQHAAHERVTYERLRRAY
                     HSGRIPSQSLVSPRVVELTPGDFAAFLDARDLIESLGFSCDPFGERTVLVRAVPQEVT
                     GSDVEGVILDAIHAPTEGYGQDTAGGRKIDAILAALACRSSVRIGVSLGREEIERLLL
                     DLDRLGSPATCPHGRPLYKRITLGDIEKWLKRKP"
     gene            4030..4962
                     /gene="miaA"
                     /locus_tag="PLT69_02585"
     CDS             4030..4962
                     /gene="miaA"
                     /locus_tag="PLT69_02585"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOJ13361.1"
                     /translation="MAEKETLIVITGPTCSGKTSCALELGRIFPVEVISADSMQVYRY
                     MDIATAKPTPEERRAVPHRLVDVVNPDEEFNAARFVRMAMRALEDIRSRGKIPMVVGG
                     TGLYVKALVHGLLEAPGCSPPLRLALKQVARKRGLSFLREMLERLDPVCAARILPNDE
                     IRLVRYLEIVLSTGRRPSELMHLHRSSPALFDAKVACIVPERERLYNSISARTGVMFD
                     RGLVEETKRLLSMGYDPSLRTMQTLAYKHVVSYVAGSIGLDECRFLVERDTKRFAKRQ
                     LTWIRSQEGNVCLPDASEAFDTVARWIAEERVSP"
     gene            5275..6039
                     /locus_tag="PLT69_02590"
     CDS             5275..6039
                     /locus_tag="PLT69_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13362.1"
                     /translation="MKQRSFAYLERLLRKKTGQAIHDYRMIEEGDRVMVAVSGGKDSL
                     VLVKILSLLRRAAPVEYELMPVYVNSGFDSGFDRVKAWIEDTLGLDVIIHDAYIREIL
                     DTVGDPDKSPCALCSRLRRGHLYSLATQMGADSIALGHHLDDIVETFLLRCFYTGQIG
                     AMAPSRVTDDGKNRIIRPLAYCTSELIDAYFSRLEVEPVTVECPARPDGKRMLVRDYI
                     KRLERDIPRVRYSLFAALGNIDARSLCTKGGDPCGS"
     gene            6027..6647
                     /locus_tag="PLT69_02595"
     CDS             6027..6647
                     /locus_tag="PLT69_02595"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOJ13363.1"
                     /translation="MRILIDPQRIKPRVVEQVGEILMDGGIIVYPTDTVYALGCIVSN
                     KHGLERISRIKASNKPKSVIFSDIKMVCDYAKMDNEAYRLMRLIFPGPYTVILPATKN
                     VPKLLQSKRKSIGVRIPDHWFCRSLVEKVGEPIITTSVPLADDRIHIDPVEIEAHLGQ
                     QVDAIVDSGILPDIPSTVISLEEDTPRIVRYGLGPTDLFEIEGAGP"
BASE COUNT         1362 a         2173 c         2142 g         1142 t
ORIGIN      
        1 ctcaacgata tatggggtgt ttccgagaaa gatgtcttcg cggtcgggga tggggggacc
       61 atcctccact tcgatgggtt cgactgggtc cccatgaaat cgaatacctc caaggacttg
      121 cgctccgtgt gggggtattc gagggaaagc gtgtatgccg tcggggaaaa cggggtcatc
      181 cttcgctacg acggagatgc atgggaggcc ttgccgagga agaccgcgca caccttgaac
      241 ggcttgtggg gaagctcgga gagagacctg ttcgcagtgg gtgacatggg caccgttctc
      301 cacttcgacg gttcctcctg gtccacgatg gacaccggca tgctcatgga tctccactcg
      361 gtatggggct cgggcgccga cgacgtgtac gcggtcggcg aaaagggcac gatgctccac
      421 tttgacggct catcgtggag ccttctccac cccatgacca cgctcgacct catggcggtc
      481 tggggaagtt ctgcggatga cgtgtacgtg gctgggcaag gatgtgtcat cctccactac
      541 ggcacctcgg ctgaccgggc catgaacacc gtcgcccatg cacgggatgc ccggagcacg
      601 gtgggcgatc ccgacatgta tgcagcacgc acagcgttcg tccccgagga tgaggcctta
      661 gacggagtga caggggagac cgtggatgcg caggtcgtct tccagagaag aaagaatgcg
      721 agcaagagcc tgctcgagtt cctggcgatc ctcgcagagg agaaaggtct ctcgagaggc
      781 gggcaggcgc tcgcggacgc agggtcggcc cccgtcggcc aaggcgggac cgacgggcgt
      841 tcgacgacga tgcttgcggc aacggaaagt caaaaggtca cggcggcggc gaatgcccgg
      901 tcagagacgc tcgtggctga cctcggggtt cctgccggcg cgatggacac taactcatct
      961 ccttcccacg tttccgtgcc ggacgatggg ggtgccggca cgcggggagc ggcatccatg
     1021 gcacccatgg acacgatgcc tgaaacgcgg gtcgccctcg gcgggaaaga acccaccccc
     1081 gttgcggcag gttccagacc atcggctgaa caaccttcga aaacccccgg cctgacccca
     1141 tccacggacc ttgcaggcat tgcgcaagag acagggcctg gaacaccagg ggtcgcctcg
     1201 acgacagagg caggagtggg gaacaacgag gtggccgagg ccgcccgctc ccaaaacgat
     1261 gccatatcgc ccgcaggcaa ggctgagggc ctcaagtctg atgaactcct cgcacgtctg
     1321 gatggttcca cctccaaacc ggcagagacc cagggttccc ttacagtcga gggtgacccc
     1381 ggcattggcg gcaacacctc tatcgaggaa accggccccc aagaagtgat catggcatcg
     1441 gccgagaccg ggaccgcccc tgccaaggct ggggacaagg acgagccggc ccctgcgggt
     1501 atcgaccagg gtgcgttcac acaccatccg tcgagcaggc agaccccctc ccccgaggtc
     1561 aggggtgagg acaggcgcac aagtgcgcga tcgctcgcat cgatcaagcc ggaaggcggc
     1621 gacgaccacg gcacgggcgg cgacatgagc gggccgggcc cggcagcgga ttcgaccggg
     1681 gcgcgcacgc ccacgtccgt ggcatccatg gcgcctgccc aggatgcggt cagcggcgcg
     1741 caaccctccg ccaaggcggg cggcacgtcg ctcggttcga cggcgactca gtgggggatg
     1801 cccacccacg ctcgacctta ctaccctgca gagtcgatct acacgcatgt gctccaatgg
     1861 atcctcgccc tctcgcttgc cggattcttc ctcttcgggc tcatcaccac cttggtgtgg
     1921 agagaagagc gggtccacag gatcctcctg cacctcacca gccaggtcac cccggagaag
     1981 tgaacccgtc gtgaccacga gcctgatgcg ccttcagcgc acaatcgata cggggcgcat
     2041 gcatcaggcc gcgcccatcg taaccgcaag gatttcctga aagattgttc ttccagcaag
     2101 gagcgtagga tcacggcgta tcgtgcaagc cgcctcatac ccggcaaacc gcctttcagg
     2161 gatgacggca ttgacctgcg caccccgcat ccgcgcctgt cgcacgacgg cccttgtgca
     2221 caaggtcgcg ttcaggtaga gcaaccttat gtcgaggata cgggtgctcc cccccgaagt
     2281 ggcgagcatg atagccgccg gcgaggtcat cgagaggccg gcgtctgtgg tcaaggagct
     2341 ggtcgaaaac gccatcgacg cacaggcggg ctcgatcgtg gtggacatca agggaaacgg
     2401 ctgcgatctc gtcagggtga ccgacgacgg ggcgggcatg acgcccgaag agctcgacaa
     2461 agccatcctc aggcacgcga cgagcaagat cggttctgcc gaggacatct ttgccatcaa
     2521 gaccctcggg ttcaggggcg aggccctgcc gagcatagcg gcggtcagca ggacgacgat
     2581 cgcgagcagg ccccgctcga cggatacggg cgctgcgctg gtgatcgagg caggggtggt
     2641 gacgaggcgc tgcctcaagg gaatgccccc gggcacgacg gcggaggtgg cagacctctt
     2701 cttcaatacc cctgccagga gaaagttcct caagacaacg gccaccgaac agcgctcgat
     2761 catcgacgtg atcgccaggt acgccctcgc ctacccaaag ctcgccttcc agctcggcat
     2821 cgacggccgc accgttatcg acgccccggg aaactcctcg catgaagaga gggccaggtc
     2881 tctcatcggc aagtcctccg cggacaggat ggcgactttc tccagggata ccgatatgtt
     2941 gcgtgttttc ggcctgctcg gccggataca ggacgcgagg aaaaaccgat ccggcatgta
     3001 cgtgttcgtg aacggccgct ccgtcaagga cggcatgata acggctgcga tcatgggggg
     3061 atatgcgggg gtgttggtca agggctccta tcccgtggcg gtcgtgttca tcgaggtcga
     3121 cccaagagag gtggacgtga acgtccaccc gacgaaggcg gaggtgaggt tcaggaaccc
     3181 ggcagctgtc ttcggcatcc tctcctcggt gatcaaggag gcggtttcct cgtccatgca
     3241 catgaacgca tcgtcatctc ctggagagga cgcctggttg accggcaggg gcatcccaca
     3301 ggcaggcagg ctcgcggagg cggcccttcc cccacacttg cacccctatg caggtcaacg
     3361 gcaaggcgaa gacggcccca cccggcctcg aggccgcccc gtcgacgcag cggagaccct
     3421 gccgctcttc ggtgcggaac gagacggtgc cgggcatgcc ttttcctatg ccgggctcac
     3481 gatactgggc agcttcagga atgcgtatct cgtcctctcc gacacggact ccctctacat
     3541 cctcgaccag catgcggcac acgagagggt gacctacgag aggctcaggc gggcgtacca
     3601 cagcggccgt atcccttccc agtccctggt ttcccccagg gtcgtggaac tgacccccgg
     3661 cgacttcgcc gcgttcctcg atgcccgcga tctcattgaa tcccttggtt tttcgtgcga
     3721 cccgttcggg gagcggaccg tcctggtcag ggcggtgccc caggaggtca ccggcagcga
     3781 cgtcgagggg gtaatcctgg acgcaatcca cgcgccaacc gaggggtatg gtcaagacac
     3841 ggcagggggc cgtaagatcg atgcgatcct cgcggcactc gcctgccgct cgagcgtgcg
     3901 catcggcgtc tcgctcggtc gcgaggaaat cgagcgtctc ctcctcgacc tcgaccgcct
     3961 cggaagccct gcaacctgcc cgcacggcag gcccctctac aagaggatca ccctcgggga
     4021 catcgagaag tggctgaaaa ggaaaccctg atcgtcatca ccgggccgac atgctctgga
     4081 aagacatcgt gcgccctcga gctcgggcgg atcttccccg tcgaggtcat atccgccgac
     4141 tccatgcagg tctacaggta catggacatc gccacggcca agcccacacc ggaagaacgc
     4201 cgggcagtac cccaccgcct ggtggatgtc gtcaaccccg acgaggagtt caacgcggcg
     4261 aggttcgtac gcatggcgat gcgcgccctc gaggacatca ggtcgcgggg caagataccc
     4321 atggtcgtgg gcggaacggg cctgtatgtc aaggccctcg tacacggcct cctggaggcc
     4381 ccgggatgtt ccccgccgct gaggctcgcc ctcaagcagg tcgcgcggaa aaggggcctg
     4441 tcctttctga gagagatgct cgagaggctc gatccggttt gtgccgcccg catcctcccc
     4501 aacgacgaga tccggctcgt caggtacctc gagatcgtcc tgtccaccgg caggcgccca
     4561 agcgagctga tgcaccttca caggtcgagc cccgccctct tcgatgcgaa ggtcgcgtgc
     4621 atcgtcccgg agcgggagcg cctctacaac tctatatccg caaggaccgg ggtcatgttc
     4681 gacaggggcc tcgtcgagga gacgaagcgg ctgctctcca tggggtacga cccttccttg
     4741 cggaccatgc agaccctggc gtacaagcat gtcgtatcct atgtggccgg ctcgataggg
     4801 ctcgatgagt gcaggttcct cgtcgagagg gacaccaaga ggttcgcgaa acgccagctc
     4861 acgtggatca ggtcgcaaga ggggaacgtg tgccttcccg atgcctctga ggccttcgac
     4921 acggtagccc ggtggatcgc cgaggaaagg gtttcaccgt gacgcatccg gaggagcaag
     4981 cctcttccct cgacagagtg caggcctttc ctcaggatcg ttcacgacgg ggacttcggg
     5041 gatgccccct cgaggccttc cctcgaggtg acggcgaact gaacgacctc cgccacgagc
     5101 cgcctgcaga atcattctcc gggaatcccg gcgcccctca ccctcgtcgg ctgtgatcat
     5161 cccggcgcca atgacccaag tgcaagcctt cgtgcgcctg ggacgtgttt tatggtatgg
     5221 cttctcacgg tgcacggtca tgcgcgtcga cagccgagac gttcgggtgg gaacatgaaa
     5281 cagagatcct ttgcatatct cgagaggctt ctgagaaaga agacaggcca ggcgatccat
     5341 gattaccgga tgatcgaaga gggagaccgc gtcatggtcg ccgtgtccgg cggaaaggat
     5401 tccctggtcc tcgtgaagat actctccctg ctgcgccgtg ccgctcccgt cgagtacgag
     5461 ctcatgccgg tctacgtaaa cagcggcttc gacagcggct tcgacagggt caaggcgtgg
     5521 atcgaggata cgctcgggct cgatgtcatc atccacgatg cgtacatcag ggagatcctc
     5581 gacaccgtgg gagacccgga caagagcccc tgcgccctgt gctccaggct caggcggggc
     5641 cacctctaca gtctcgccac acagatgggc gccgactcca tcgcgctcgg tcaccacctg
     5701 gacgacatcg tcgagacctt cctgctcagg tgcttctaca ccggccagat aggagcgatg
     5761 gcgccgtcac gggtgaccga cgacgggaag aaccgcatca tcaggcctct cgcctactgc
     5821 acctcggagc tcatcgatgc ctacttcagc cgcctcgagg tcgaacccgt gacggtggag
     5881 tgcccggcac ggcccgacgg caagcgcatg ctcgtaaggg actacatcaa acgcctcgaa
     5941 cgtgatatac ctcgggtgag atactctcta ttcgccgccc tgggaaacat cgacgccagg
     6001 agcctgtgca cgaagggggg agacccatgc ggatcctgat tgaccctcaa cggataaaac
     6061 cgcgggtggt cgagcaggtt ggcgagatac tcatggatgg cggtatcatc gtgtacccca
     6121 cggacaccgt gtacgccctg ggctgcatcg tgagcaacaa gcacggtctc gaacggataa
     6181 gcaggatcaa ggcgtcgaac aaacccaaga gcgtcatctt ttccgatatc aagatggtgt
     6241 gcgattacgc gaagatggac aacgaggcct acaggctcat gcgcctcatc tttccagggc
     6301 cctacacggt gatcctgccc gcgaccaaga acgtgcccaa gctcctgcag tcgaaacgca
     6361 agtccatcgg cgtcaggatt cccgatcact ggttctgcag gtcgctcgtg gagaaggtcg
     6421 gggaacccat catcaccacg agcgtacccc ttgcggacga ccgtatacac atagatcccg
     6481 tcgagatcga ggcccacctc gggcagcagg tggatgccat cgtcgacagc ggcatcctgc
     6541 ccgacatccc ctccacggtg atctccctcg aggaggacac cccgaggata gtccggtacg
     6601 gtctcggacc caccgacctg ttcgagatcg agggggccgg gccttgaaga agatgctcat
     6661 caacgccaag tttcccgagg aggtaagggt cgccgtcgtg gaagacggcc tgctcaccga
     6721 gttccacatg gagagcccgc tcaaggagca actcaaggga aacatctaca aggcgcgcat
     6781 agcgaaggtc gagcacggcc tgaacgccgt cttcgtcga
//
LOCUS       DAOSCR010000058         6715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7303_length_6715_cov_3.516517, whole genome shotgun sequence.
ACCESSION   DAOSCR010000058 DAOSCR010000000
VERSION     DAOSCR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7303_length_6715_cov_3.516517"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLT69_02600"
     CDS             complement(<1..1233)
                     /locus_tag="PLT69_02600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ13364.1"
                     /translation="MDEREGRIVKVSGPLVVAKGLDGGRMYEVVRVGQYRLFGEIIEI
                     RGDEYSIQVYEETEGLGPGQPVLRTGEPLSVELGPGLLRSIYDGIQRPLDVLARDFGD
                     FIVRGAERPALDRSRKWSFVPAARAGDKVEPGDILGEVQETELISHKIMVPPGRSGVV
                     KEIGPVVGTVDTQVAVIEGPEGPFGVTMLQKWPVRVPRPVRRKLHPKEQLVTGQRIID
                     MFFPIVKGGTACVPGPFGSGKTVVQHQLAKWADARIVVYVGCGERGNEMTDVLMEFPR
                     LMDPASGKPLMERTVLVANTSNMPVAAREASVFTGITIAEYFRDMGYSVALMADSTSR
                     WAEAMREISGRLEEMPGEEGYPAYLGSRIAAFYERGGYAVCLGGGERTGAVSIIGAVS
                     PPGGDFSEPVVQATLRVVK"
     gene            complement(1322..1642)
                     /locus_tag="PLT69_02605"
     CDS             complement(1322..1642)
                     /locus_tag="PLT69_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOJ13365.1"
                     /translation="MAKTVAVGPRAWVAPFRGVGFESVQVEEPEDLLKALLAIAGQRD
                     VSLVLVSEVLAGKAPAAIEEFRKRSRAVICALPTHEGATGTGYEETRRLVESSLGIDL
                     LGGA"
     gene            complement(1635..2636)
                     /locus_tag="PLT69_02610"
     CDS             complement(1635..2636)
                     /locus_tag="PLT69_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOJ13366.1"
                     /translation="MTGLAVQPVPGYVRGWGFVCGGIAVLEERFRDRDFFMGLLALEE
                     VGDMIPHLQDTVLREYLTPGAEWEDFTEIAKRCFYDTALAVRSMCPSSGPVDVFVAPY
                     DFMNLKTSLAQNTSFPYPFVVFPEDQLVALAKGELPEAPESLKEWPGWSTSEIQHLAP
                     LDLDILLDGAYLRYLLHAAEGIGSDFIRACVRERVKAKVVTLLWRAHALGIPLKRVEN
                     HLQPLGELTGLVEDLALQSNPQAWAALVGGEIGDVLAEVQEWGSVDRVPEFASKVDDR
                     LLSVAALGGMQTSGPEAVFSFMAGFEHQLRLLTLVVTGRLGGVDRDGLRRRIGGAHG"
     gene            complement(2633..3223)
                     /locus_tag="PLT69_02615"
     CDS             complement(2633..3223)
                     /locus_tag="PLT69_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOJ13367.1"
                     /translation="MNGIDRIRSAILAEAETEAGRIVEAAKKAAFERVQSEKDKITRT
                     FDLEFSAKAREVDARLERELALFRGEAGKRVLAKKNELVRRVFETAKAEILSWPRARY
                     ESLMERLVEKASGGAPGRLIVHPDDEDVFRALLERVNAGRAASGRIDVDGVDRLRQRG
                     GFVFVGSSYEVDMRIETLIEVLELEMAPEIARELFS"
     gene            complement(3242..3760)
                     /locus_tag="PLT69_02620"
     CDS             complement(3242..3760)
                     /locus_tag="PLT69_02620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOJ13368.1"
                     /translation="MTPDMIEIGRGLCYAGAGLAFGLGGSGSAWGIATASHQAAGVMR
                     EKPELFGRMLVMIALPGTQGFYGFIIAVLMMTQTGLIGGAELRITPGTGIAFFFIGLA
                     SGLALMVSAMKQGEASAAGISLIARRPEAAGRAILFPAFVETYAVVALLATILFITFL
                     TQGVALEYIGLS"
     gene            complement(3797..5854)
                     /locus_tag="PLT69_02625"
     CDS             complement(3797..5854)
                     /locus_tag="PLT69_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13369.1"
                     /translation="MAIDPIRKVTVTVCRKNSLRLLREINRLGVMEVVDLAPDNAGGL
                     LDRCHASTDEADEKIHQVDFILNLLDGHAPERVGLIEGLAPMPIVTTNEELSRILSSY
                     DLQKTFLWAKDLDETCRTSERVIQEMETEIESLSALADLEFTPAEVLDLKRTTVIFGR
                     LPREALSLLADGQGVWGLCAWEELPPEATMPLHAGRDMARVVLVALKEEAAEIQAALR
                     GAGFEEIPLPRLKTTARERIDELRDTVEMYKEKTGDALERIQDFTKGRREGEGRRPLK
                     ILRAYWQNVRDRSLALQKGLVGRWVHVVSGYVRLRDIPRLTALVERDFPESMLVLEDP
                     GPGDDVPVSLHEKKPSRPLSLLVEMFGLPPYRSFDPTPFLMFNFYVFFGICFSDVFYG
                     VMLTAVSAFLRARTKASSGVNRFVEILFFGGISSIVFGALMGSWFGDLYRPEYLGEGN
                     LLLRTRDALMVIDPMAKTVLALLVVLAIGVVNQFYGITLRMYGQLRRGDWLGAFSDGV
                     CWIGALCGVLVVVAGVFADVPHDIARIGVWLLAVSSVCLVLTQGRGVENPLKRIAVGI
                     VSLYGIVGSYGLTSFAGDVLSHCRLLALGLTTSIVAMAFNLMAGMLRDIPYVGALLFV
                     AVLAVGHVFNFAISVLGAFVHSMRLVYVEFFGRFYEAGGRPFRPLGFDTPWCVLRKDA
                     EEA"
     gene            complement(5857..6168)
                     /locus_tag="PLT69_02630"
     CDS             complement(5857..6168)
                     /locus_tag="PLT69_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13370.1"
                     /translation="MSEVVRRIVEVEREADAIIEAAHRKAADREREALSEIDAFRAGA
                     LKDLASRIDSYEAQAMARQADLLLGARRELEEELLRLEAIPEERIASLAKKVVERLEG
                     T"
BASE COUNT         1193 a         2406 c         1965 g         1151 t
ORIGIN      
        1 cttgaccacc cgcagggtcg cctgcaccac cggctccgag aaatcgccgc ccggcggcga
       61 gacggcgccg atgatggaga ccgccccggt ccgctcgcct ccgcccaggc acaccgcgta
      121 accacccctc tcgtagaagg ccgctatgcg cgaaccgaga taggcgggat acccctcctc
      181 gcccggcatc tcctcgagcc tcccggatat ctcgcgcatg gcctccgccc acctgctcgt
      241 cgagtcggcc atgagggcaa ccgaataccc catgtccctg aagtactcgg cgatcgtgat
      301 gcccgtgaac acgctcgcct ccctcgccgc gacaggcatg ttcgacgtgt tcgcgacgag
      361 cacggtccgc tccatgaggg gcttgcccga ggcggggtcc atgagcctcg gaaactccat
      421 gaggacgtcg gtcatctcgt tgccccgctc gccgcacccg acgtagacca cgatcctcgc
      481 atccgcccat ttcgcgagct gatgctgaac caccgtcttc ccgctgccga agggcccggg
      541 gacgcaggcg gtgcctccct tcacgatggg gaagaacatg tcgatgatcc tctggcccgt
      601 gaccagctgc tccttggggt gaagcttgcg cctgacgggc cgcggcacgc gcacgggcca
      661 tttctggagc atggtcacgc cgaacggccc ctccggcccc tcaatgacgg caacctgcgt
      721 atcgaccgtt ccgaccaccg gcccgatctc cttgacgacg cccgacctgc ccggcgggac
      781 catgatcttg tggcttatga gctccgtctc ctgcacctcg cccaggatgt cgcccggttc
      841 caccttgtcc ccggccctgg cagccggcac gaaggaccac ttcctcgaac ggtcgagcgc
      901 cggcctctcg gcgcccctga caatgaagtc gccgaaatcc ctcgccagga cgtccagggg
      961 cctctggatg ccgtcgtaga tcgacctgag gaggccggga ccgagctcca cggaaagggg
     1021 ctcgccggtc ctgagcaccg gctggccggg ccccaagccc tccgtttcct cgtagacctg
     1081 tatcgagtac tcgtcccccc ggatctcgat gatctcgccg aagagacggt actggcccac
     1141 gcgcaccacc tcgtacatgc gcccaccgtc caggcccttg gcgactacaa ggggcccgga
     1201 caccttcacg atcctgccct cgcgttcatc catgtgcatg ttcccttcct gaccaccaca
     1261 tcgcttcagc cacgcccccg cggccccctg tatgcacccc tcgagcccac ctacgagggg
     1321 gtcatgcccc tcccaggagg tctatgccga gggaggactc gacgagtctc ctcgtctctt
     1381 cgtatcccgt tcccgtggcg ccctcgtgcg tggggagggc gcagatcacc gcgcggctcc
     1441 tcttcctgaa ctcctcgatc gcagccggcg ccttccccgc taggacctcg ctcacgagca
     1501 cgagggagac gtcccgttgc ccggctatgg ccaagagggc cttcaggagg tcctcgggtt
     1561 cttcgacctg cacggactcg aagcccaccc ccctgaacgg agcgacccag gcccttggcc
     1621 ccaccgcgac cgtcttagcc atgggcgccc ccgatccttc gcctcaggcc gtcgcgatcg
     1681 accccgccga gcctgcctgt cacgacgagc gtgagaagcc tcagttggtg ctcgaacccg
     1741 gccatgaacg agaagaccgc ctcgggccca gaggtctgca tccctcccag tgccgcaaca
     1801 gacaacagcc tgtcgtccac cttggaggcg aactcgggaa cccggtcaac cgatccccac
     1861 tcctgcacct ccgccaggac gtccccgatc tcgcccccca cgagggcggc ccaggcctgg
     1921 gggtttgact ggagcgcgag atcctccacg aggcccgtga gctcacccag gggttggagg
     1981 tggttctcca ccctcttcag gggtatcccc agggcatgcg ccctccagag aagggtcacc
     2041 accttcgcct tgacacgctc gcgcacgcat gccctgatga agtctgaccc gatcccctcg
     2101 gcggcgtgga ggagatacct gaggtatgcg ccgtcgagga ggatgtcaag gtccaggggg
     2161 gcaaggtgct ggatctcgga cgtggaccat ccgggccact ccttgaggct ctccggtgcc
     2221 tccggcagct cccccttcgc caaggccacg agctgatcct cgggaaagac cacgaagggg
     2281 tagggaaacg aggtgttctg cgccagcgag gtcttcaggt tcatgaagtc gtacggggcc
     2341 acgaacacgt cgacgggccc gctcgacggg cacatcgagc gcacggccag ggccgtgtcg
     2401 tagaagcacc gcttggcgat ctccgtgaag tcctcccact cggccccggg cgtgaggtat
     2461 tccctgagga cggtgtcctg gaggtggggg atcatgtccc cgacctcttc gagcgcaagg
     2521 aggcccatga agaaatccct gtccctgaac cgttcctcga ggacggcgat gcccccgcac
     2581 acgaagcccc accctcggac gtatccggga accggctgga ccgcaagccc tgtcatgaaa
     2641 agagctcccg cgctatctcg ggggccatct ccagctcgag cacctcgatg agggtttcga
     2701 ttctcatgtc cacctcgtag gaagaaccca cgaacacgaa cccgccccgc tgtctgagcc
     2761 gatcgacgcc gtccacgtcg atcctcccgg aggcggccct cccggcgttc acccgctcga
     2821 ggagcgccct gaacacgtcc tcgtcgtcgg ggtggacgat gaggcgcccg ggggctccac
     2881 cagaggcctt ctccaccaag cgctccatca gcgactcgta cctggccctc ggccaggaaa
     2941 ggatttccgc cttcgcggtt tcgaacaccc tcctcacgag ctcgtttttc ttcgcgagca
     3001 cgcgtttccc ggcctccccc ctgaacaggg cgagttcccg ctcgagcctt gcgtccacct
     3061 ccctggcctt tgcggagaac tcgagatcga acgttctcgt gatcttgtcc ttctcacttt
     3121 gaacccgttc aaaggcggcc ttcttcgcag cctcgacgat gcggccggcc tcggtttcgg
     3181 cctccgcgag gatggcggac ctgatccgat ctatcccgtt cacggtcgaa ccctcatccc
     3241 ctcatgatag gcctatgtac tcgagggcga cgccctgggt gaggaacgtg atgaacagga
     3301 tggtcgcgag cagggcgacc accgcgtacg tctccacgaa tgcggggaag aggatggccc
     3361 tgcccgcagc ctcgggcctc ctcgctatga gcgagatgcc ggcggccgag gcctccccct
     3421 gcttcatcgc cgagaccatg agcgcgagcc cgcttgcgag ccctatgaag aagaaggcga
     3481 tgccggtgcc gggcgttatc ctgagctcgg ccccccctat gagacccgtc tgggtcatca
     3541 tgagcacggc gatgatgaac ccgtagaaac cctgggtgcc gggcagggcg atcatgacga
     3601 gcatcctgcc gaacagctca ggcttctccc tcatcacgcc cgcggcctgg tgggacgccg
     3661 tggcgatccc ccaggcggac ccgctcccgc cgagcccgaa cgcgagcccg gcacccgcat
     3721 agcagaggcc cctgcctatc tcgatcatgt caggtgtcat actccctcct cctctccccg
     3781 attcgggtac gcatctctat gcctcttcgg cgtccttcct caggacgcac catggggtgt
     3841 cgaacccgag aggcctgaac ggacggcctc cggcttcgta gaatctcccg aagaactcga
     3901 cgtacacgag cctcatggaa tgaacgaagg ccccgagcac gcttatggcg aagttgaaga
     3961 cgtggccgac ggcgagaacc gccacgaaga gcaacgcccc gacgtagggg atgtccctga
     4021 gcatgccggc catgaggttg aaggccatgg ccacgatcga ggtcgtgagc cccagcgcaa
     4081 gcagcctgca atgggaaagc acgtccccgg cgaacgaggt cagcccgtaa ctccccacga
     4141 tgccgtacag gctcacgatc cccacggcga tcctcttgag cgggttttcg acacccctgc
     4201 cctgggtcag gaccaggcag acggagctca ccgccaacag ccacacgcct atcctcgcga
     4261 tgtcatgcgg aacatccgcg aacaccccgg ccacgaccac gaggaccccg cagagcgcgc
     4321 cgatccagca gaccccgtcg gagaaggcgc cgagccagtc tccccgcctc agctggccgt
     4381 acatcctaag cgtgatcccg tagaactggt tcaccacccc tatggcgagc accaccagga
     4441 gcgcgagcac cgtcttggcc atggggtcga tgaccatgag cgcatccctt gtcctcagga
     4501 gaaggttgcc ctctcccagg tactccgggc ggtagaggtc gccgaaccac gagcccatga
     4561 gcgcgccgaa gacgatgctc gatatcccgc cgaagaacag gatctcaacg aaccggttca
     4621 cgccgctgga tgccttggtc ctcgccctca aaaacgcgct taccgccgtg agcatgaccc
     4681 cgtagaagac atccgagaag cagatgccga agaagacgta gaaattgaac atgaggaaag
     4741 gggtggggtc gaacgagcgg taggggggca ggccgaacat ctccacgagc aggctcaacg
     4801 gcctggaggg cttcttctcg tgaaggctca ccgggacgtc atccccgggg ccggggtcct
     4861 cgagcacgag catggactcg gggaaatcac gctcgacgag ggctgtcaag cgcgggatat
     4921 ccctcagcct gacgtacccg gataccacgt gcacccagcg gcccacgagg cccttctgca
     4981 gcgcgagcga acgatccctg acgttctgcc agtaggccct caggatcttg agcggccttc
     5041 ggccctcacc ctcgcgcctg cccttcgtga agtcctgtat gcgctcgagg gcatcgcccg
     5101 tcttttcctt gtacatctcg acggtgtcac gaagctcgtc gatcctctcc ctcgccgttg
     5161 tcttcaagcg gggcagcggg atctcctcga agccggcgcc cctgagagcc gcctggatct
     5221 cggcggcctc ctccttcagc gcaaccagga cgaccctcgc catgtcccgg cctgcatgaa
     5281 ggggcatggt cgcctcgggc gggagctctt cccatgcaca cagaccccat accccttgtc
     5341 cgtctgcgag cagtgacagg gcctccctcg gcaggcggcc gaagatgacc gtggtcctct
     5401 tgaggtccag aacctccgcg ggtgtgaact cgaggtcggc cagggccgag agcgactcga
     5461 tctccgtctc catctcctgg atgaccctct ccgaagtcct gcaggtctcg tcgaggtcct
     5521 ttgcccacag gaaggtcttc tggaggtcgt acgaagaaag gatcctggag agttcctcgt
     5581 tcgtggtcac gatgggcatg ggcgccagtc cctcgatcag cccgacgcgt tcgggagcgt
     5641 ggccgtccaa gaggttcagt atgaagtcga cctggtgtat cttctcgtca gcctcgtccg
     5701 tggatgcatg gcacctgtcg aggaggccgc cggcgttgtc cggcgcgagg tccacgacct
     5761 ccatcacgcc gaggcggttt atctccctca agaggcgcag gctgttctta cggcagaccg
     5821 tgacggtgac cttcctgatg gggtcgatcg ccatcctcag gtgccctcca gacgctcgac
     5881 gaccttcttt gccagagaag cgatcctttc ctcggggatg gcctccagcc ttaagagttc
     5941 ttcctcgagc tcacgcctcg caccaaggag gaggtctgcc tgcctcgcca ttgcctgggc
     6001 ctcgtaggag tcgatcctcg atgcgagatc cttgagcgca cccgcgcgga aggcgtcgat
     6061 ctcgctcagc gcctcgcgct ctctgtcggc ggccttccgg tgggcggcct ctatgatggc
     6121 atccgcctct ctctcgacct cgactatgcg cctgacgacc tcggacacgg gttcctcctt
     6181 gttcggaaca accctcgaac cgggacacga gcaccgactg ccggtcgctc tccaccatcc
     6241 agcgagggtc ttcttcccgt cacctcccgg tgaccataaa agggcacgca cagccccggt
     6301 gggcctcagg atgaatacca taacaaaacg aaaaccgcat gcgccacagg aaagtgatcc
     6361 gcctggacat ccgaaggcga gggccccgtg taccgcgggg gacacgggtc tttctcgacg
     6421 accaccccgg tggcaacctg ccggcgtcgt cccctcacgg cacaaggaca cgcacgcgcg
     6481 gcatcacaga ccctccccga tccagacagg cttcacatgc cacgacctct cgttctccct
     6541 catcttcacc aacccctcca gggggcaggg cgttgaccgg catgcacggc cgcacaggct
     6601 caccggccat gacaggagcc gcacaagggg gcttgtcccc atggtcatcc ggagaaacgg
     6661 gttttcctcc ctgttcatct ttttcggggc ccttcagaaa ggcgcactga agaag
//
LOCUS       DAOSCR010000059         6704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7326_length_6704_cov_2.792450, whole genome shotgun sequence.
ACCESSION   DAOSCR010000059 DAOSCR010000000
VERSION     DAOSCR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7326_length_6704_cov_2.792450"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLT69_02635"
     CDS             <1..1054
                     /locus_tag="PLT69_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOJ13371.1"
                     /translation="AEIHEDGSFVITKHPKTGGLVSVDTVKAQLLYEIRGPAYLTPDV
                     TAHFDSVSLAQEGPDRVSVRGAKGSPPPDTLKVCINTLAGYRNSVTVVLTGLDVERKA
                     RVVTEALFDAIGPADSFQVCDVQLVRSDREDPPTNEQAFAYLRITVIDPDQKKADRVS
                     SAVVELALANIPGFTLTAPPSRATPAVVYWPALVGASRVRQAVVMNGARTEVDAPNRV
                     PSAEPFVPGAVDLPRPPGGPKARVPFGRVFATRSGDKGGDANLGVWARTSDAYAFLRS
                     YLDVGELKKLCTDLAPYEIERYELPNLLALNFYIKGMLGLGAASSVRMDPQAKTLGEY
                     LRARVVEVPESIVPGR"
     gene            complement(1350..1802)
                     /locus_tag="PLT69_02640"
     CDS             complement(1350..1802)
                     /locus_tag="PLT69_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13372.1"
                     /translation="MFYFELLDLIKNNANELTRRACKEISTREETRGYRKIPADVLYE
                     RVFDVYNNLSGWLGQDDHTMSQIRTVYTELGRKRYHEGIPLHEVVLAFMLVKRILWEF
                     IQERQFFETTFEMGQALELNNRVVFFFDRVIYFVSMGYEDELLKNMKK"
     gene            2225..2884
                     /locus_tag="PLT69_02645"
     CDS             2225..2884
                     /locus_tag="PLT69_02645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ13373.1"
                     /translation="MDVKDIDRVARKGFLDPEEGRALHDLAMDAARRGPVLEIGGYCG
                     KSTLYLGLGCQKSGGILFSVDHHRGSEEQQPGQPYFDPDLFDPRLERVDTFPFFRRAV
                     EEARLENTVVPIVASSRVAARMWATPLAMLFIDGGHTYEAAFDDYVSWSGFLTPGGIL
                     AVHDIFLDPQKGGQAPRFVYSLAVASGLYEELPMVGTLGVLRRRAPGEVPPEISSRRD
                     W"
     gene            complement(2867..4459)
                     /locus_tag="PLT69_02650"
     CDS             complement(2867..4459)
                     /locus_tag="PLT69_02650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13374.1"
                     /translation="MRIALIATPYPLEEAPSPPLGLSYAAAALESVGATVRILDFIVS
                     RYTPEKLRRFLDAFEPDAVGAGCVTMNYPDAERIIKDVKRFRPDTVTMMGGPHVSFDV
                     EGTFASCPELDLVVIGEGEDTLMELAPVLTDRSAWGAVRGIAYREDGEVVRTGPRELI
                     HDLDRLPLPARHLLPVSRYLALGYPVSIITSRGCPNRCIFCLGRKMVGFKGRYRDPGL
                     VVDEIEHILSLGFTRINVADDLFTADRKRVAMLCEEILGRGVKFGWSAFARVNTVDPE
                     TLKIMRKAGCDCISFGIESGNPEMLKRIRKGITLEQARQAVAWCKEADILPHASFMVG
                     LPGETRQTLEDTHRFATELNIAYGYHFFAPFPGTTVREELENYDIEILTNDWGRYDAN
                     SPVVRTSALSPEEMIEFVKEYDRFNEAIWEESERRVAEGTCTEREYLLVEGNKKLKLV
                     YTVLSSDLVEEASIERVDHASPQDLLASRLAAVTGTDENFTKRVIGEFFQAGYLRHSL
                     EGSRFRYRWTHNLEVDELPIAP"
     gene            complement(4470..5192)
                     /locus_tag="PLT69_02655"
     CDS             complement(4470..5192)
                     /locus_tag="PLT69_02655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ13375.1"
                     /translation="MRRDHRPYYLKRLDLLIRSFYVEHFLRPHFRRLGRGCTFMKPWH
                     VHVFGYPVDLGDFANVITTPDNRVRLTVWGRTKDDGFISIGRYCLICPGVRISAATGI
                     TVGDSCMMASGVYITDSDWHGIYDRLDYIGASAPVTIGSNVWLGDSVIVCKGVTIGDN
                     SIIGAGSVVTSDVPSNVIAAGNPARVIRELDGKTPMRTRAEWFTDPAKLESDIASIDR
                     DMLSGNSLIGWLRSIVFPRIGD"
     gene            complement(5197..6318)
                     /locus_tag="PLT69_02660"
     CDS             complement(5197..6318)
                     /locus_tag="PLT69_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenyltransferase domain-containing protein"
                     /protein_id="HOJ13376.1"
                     /translation="MERDQALQGPAIELDLLASSIASLQRPSGEIPWHEGGKTDPWDH
                     VEAAIGLTIGGRYEEARKAFEWLASRQLEDGSWYSAYRDGVPEDLTRESNMSSYIAVG
                     VFHYYLVTRDKAFLEYIWPTVSAGIDFAVGLQAPTGAVYWARNPAGEVDPVALLTASS
                     SVFMSLKCALVLALLLGESRPRWQSALKRLGDAIRYRPHLFDRTKSRYSMDWFYPVLC
                     GALTGLEAKNRIERFWNRFMVEGLGTRCVSDNPWITVAETSELILTLAAMGEHEEARR
                     VLGWIHDKRYDDGSYWCGFTFPDMVVWPEEKLTWTNAAVMMAFDALNHVTPASQLFSH
                     TFWEAGKGDSSHMYRFLKKHHPVFSIKRAFYSDAADMRA"
     gene            complement(6308..>6704)
                     /locus_tag="PLT69_02665"
     CDS             complement(6308..>6704)
                     /locus_tag="PLT69_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOJ13377.1"
                     /translation="RVLKPGKSLVVSVPRYLPERICWALSEEYHTEKGGHVRIYKTSE
                     LVALLERAGLKCIGRGWAHALHSPYWWIKCMVGHKNDQALPVRLYHKFLVWDIMKKPR
                     LTRTLDRLLNPVIAKSVVLYCTKGDSHGT"
BASE COUNT         1089 a         2197 c         2222 g         1196 t
ORIGIN      
        1 ggcggagata cacgaggacg ggtccttcgt gatcaccaag caccccaaaa cgggggggct
       61 cgtgagcgtg gacacggtga aggcccagct cctctacgag atacgggggc ccgcgtacct
      121 gacgccggac gtcacggccc acttcgacag cgtgagcctg gcccaggaag gccccgaccg
      181 cgtctcggtg agaggggcga aggggtcgcc tcctcccgac acgctcaagg tttgcatcaa
      241 cacgctcgca ggctaccgca actcggtcac ggtcgtgctc acggggctcg acgtggaacg
      301 gaaggcccgc gtggtgaccg aggcgctctt cgacgcgatc gggcctgccg actcgttcca
      361 ggtgtgcgac gtccagctcg tgcgttccga cagggaggac ccgcccacga acgagcaggc
      421 cttcgcctac ctccgcataa cggtcatcga ccccgaccag aagaaggccg acagggtctc
      481 cagcgccgtg gtcgagctgg cgctcgccaa catcccgggc ttcacgctca cggcgccgcc
      541 gtccagggcc acgcccgcag tcgtgtactg gccggccctg gtcggggcct cgcgggtgag
      601 gcaggccgtt gtgatgaacg gcgcccgcac cgaggtggac gcgccgaacc gcgtgccgtc
      661 ggcagagccc ttcgtcccgg gcgccgtcga cctgccgcgg ccgccgggcg gcccgaaggc
      721 gagggtgccc ttcgggaggg tgttcgcgac caggtccggg gacaagggag gggacgcgaa
      781 cctcggcgtg tgggcacgga cgagtgatgc gtacgcgttc ttgaggtcct acctcgacgt
      841 cggcgagctc aagaagcttt gcacggacct ggccccctac gagatcgagc gctacgagct
      901 gcccaacctg ctcgccctga atttctacat caaggggatg ctcggcctcg gcgcagcctc
      961 gtccgttcgg atggatcccc aggcgaagac cctgggtgaa tacctgcgcg cccgcgtggt
     1021 ggaggtgccc gagtccatcg tgccgggccg ttgaggtgcg ggcctgagtg aggtgcgggt
     1081 ggaacgctca aggggcgggc ccatcggcga acgtgcagac cctgcattca ccccgaggat
     1141 cccctcgggc ggggccgaag cccgcggcct gaccggggag cgggcgttcg gagagagcga
     1201 ccggctgcgt tgtctctccg gttcagggag gcggccacgc acctgccgtc acgtcggccg
     1261 tccccgggga acgcgctccg gacgggtcac ttccgcgccc gtggggagcg ctctccgggc
     1321 gaaccttgag gcatgggtcc aggcggggcc tacttcttca tgttcttgag gagctcgtcc
     1381 tcgtaaccca tggacacgaa gtagatgacc ctgtcgaaga agaagaccac gcggttgttg
     1441 agttcgaggg cctggcccat ctcgaaggtg gtctcgaaga actggcgctc ctggatgaac
     1501 tcccagagta tccgcttcac gagcatgaac gccagcacca cctcgtgaag ggggatcccc
     1561 tcgtggtacc tcttcctgcc cagctccgtg tagacggtgc ggatctggct catggtgtgg
     1621 tcgtcctgcc cgagccagcc gctcaggttg ttgtacacgt cgaagaccct ttcgtagagg
     1681 acgtccgcgg ggatcttccg gtaccccctc gtctcctccc tggtggatat ctccttgcac
     1741 gccctcctgg tgagctcgtt cgcgttgttc ttgatgagat cgagcaactc gaaatagaac
     1801 atgatccctc ctttccatgc aaaggtccct gaccccactc ccggcataca cgaaagagcc
     1861 ctgtcacccc gttcgtcgcc gtgccactgc cgcgaaagcg gactctcgtc gtaatcttca
     1921 agatataaaa ggggggcctg ccgcggtcaa gggcatgggc gaaagaggga cggggccgcc
     1981 tcggcgtggc gctcggcctc aaggaggcgg ggatgtgccg tggtctccgg agggggaggc
     2041 cgccctctcg accggtcagc ggagagcctg cctcgatggt tcaacgccga caaggcggca
     2101 accaggtctc cacggtacgc aggcactcgc gcgcatcccc tttcgcccgg cgtgtccccg
     2161 gcctgaaatc gcttgacttc caaggtccgg cccgcttact tcatgggagg aaagcgggtg
     2221 cacggtggac gtgaaagaca tcgacagggt ggcgcgaaag ggttttctcg accccgagga
     2281 ggggcgcgcg ctccatgacc tggccatgga cgccgcacgc agggggcctg tcctggagat
     2341 aggggggtac tgcggcaagt cgacgctcta cctcggcctg ggctgccaaa agagcggcgg
     2401 gatcctgttc tcggtggacc accacagggg gtcagaggaa cagcagccgg gccagcccta
     2461 cttcgacccg gacctcttcg acccccgcct cgaacgggtc gacaccttcc ccttcttcag
     2521 gagggccgtc gaggaggcgc gcctggagaa cacggtggta cccatcgtgg cgagctcgag
     2581 ggtggcggcc cgcatgtggg ccacacccct tgccatgctc ttcatcgacg gcggacatac
     2641 ctacgaggct gccttcgacg actatgtctc gtggtcgggg ttcctcacgc ccggcggtat
     2701 cctggctgtc cacgacatct ttctcgaccc tcaaaagggc ggtcaggcgc cccgtttcgt
     2761 gtattcgctc gccgtcgcgt caggcctcta cgaggagctc cccatggtgg ggacgctcgg
     2821 tgtgctcagg aggcgcgcgc caggggaggt tcccccggag atatcgtcaa ggcgcgattg
     2881 gtagctcgtc cacctcgagg ttgtgagtcc accggtaccg gaagcgcgat ccctcgaggg
     2941 agtgcctgag gtagcccgcc tggaagaact cgccgatcac cctcttcgtg aagttctcgt
     3001 cggtcccggt tacggccgcg agcctcgatg cgaggaggtc ctggggggat gcgtggtcga
     3061 cacgctctat cgacgcctcc tccacgaggt cgctcgagag cacggtgtag acgagcttga
     3121 gcttcttgtt cccctccacg aggaggtact ccctctccgt gcacgtgccc tcggccacgc
     3181 gccgctcgct ctcctcccag atggcctcgt tgaaccggtc gtactccttg acgaactcga
     3241 tcatttcctc cggcgagagg gcggaggtcc tcaccactgg cgagttggcg tcgtacctgc
     3301 cccagtcgtt cgtgaggatc tcgatgtcgt agttctcgag ctcctctctc accgtcgtcc
     3361 cggggaacgg cgcgaagaag tggtacccgt aggcgatgtt gagctcggtg gcgaacctgt
     3421 gggtgtcctc gagggtctgt ctcgtctcgc cgggcagccc caccatgaag gacgcgtgcg
     3481 gcaggatgtc cgcctccttg caccaggcca ccgcctggcg cgcctgctcg agggtgatcc
     3541 ccttgcggat gcgcttgagc atctcggggt tgcccgactc tatgccgaag cttatgcagt
     3601 cgcagccggc cttgcgcatg atcttcaggg tctcggggtc cacggtgttg accctcgcga
     3661 acgcgctcca gccgaacttc acccccctgc ccaggatctc ctcgcacagc atggccacgc
     3721 gtttcctgtc cgcggtgaag agatcgtcgg ccacgttgat cctggtgaaa cccagggaga
     3781 ggatgtgctc gatctcgtcc accacgaggc ccgggtccct gtagcggccc ttgaacccca
     3841 ccatcttccg gccgagacag aagatgcacc ggttgggaca acccctgctc gtgatgatgc
     3901 tcacggggta gcccagggcc aggtacctgg agacgggcag gaggtgcctg gccggcagcg
     3961 gcagcctgtc caggtcgtgg atgagctccc tgggcccggt gcgcacgacc tcgccgtcct
     4021 cgcggtaggc gatacccctg actgcgcccc atgcgctgcg gtcggtcaag accggcgcga
     4081 gctccatgag cgtgtcctcg ccctcgccga tcacgacgag gtcgagctcc gggcacgagg
     4141 cgaacgtgcc ctccacgtcg aaggagacgt gcggcccccc catcatggtg acggtatccg
     4201 ggcggaagcg cttcacgtcc ttgatgatgc gctcggcatc cgggtagttc atggtcacac
     4261 agccggcgcc caccgcgtcc ggctcgaacg cgtcgaggaa gcgtcggagc ttttccggcg
     4321 tgtacctgct cacgatgaag tcgaggatgc ggaccgtcgc gcccacggac tccagggccg
     4381 cggccgcgta cgagagaccg aggggagggg aaggcgcctc ctccagggga tacggcgttg
     4441 cgatgagtgc tattctcatg tcctgcacct caatccccga ttctggggaa gacgatcgat
     4501 ctgagccagc cgatcaggct gttgcccgag agcatgtcgc ggtctatgga cgcgatgtcg
     4561 ctctcgagct tcgccgggtc ggtgaaccac tcggcgcggg tcctcatggg cgtcttgccg
     4621 tcgagctccc tgatcacgcg ggccgggttc ccggccgcga tcacgttcga aggaacgtcg
     4681 gaggtcacca ccgagccggc gccgatgatg ctgttgtcgc cgatggtcac gcccttgcac
     4741 acgatcacgc tgtcgccgag ccagacgttc gatccgatgg tcacgggagc ggaggcgccg
     4801 atgtagtcca ggcggtcgta tatgccgtgc cagtccgagt cggtgatgta gacgccgctg
     4861 gccatcatgc agctgtcccc caccgtgatc cccgtcgccg cgctgatgcg gacccccggg
     4921 cagatgaggc agtagcgccc tatggagatg aacccgtcgt ccttggtcct gccccagacc
     4981 gtgagcctca cgcggttgtc cggcgtggtg atgacgttcg cgaagtcgcc gaggtccacc
     5041 gggtacccga agacgtggac gtgccagggc ttcatgaagg tacaccccct gccgagccgc
     5101 ctgaagtggg gcctcaggaa gtgctcgacg tagaaggacc tgatcaggag atcgagccgc
     5161 ttgaggtaat atggcctgtg gtccctgcgc acgacctcac gccctcatgt ccgcggcgtc
     5221 ggaatagaag gccctcttga tgctgaacac ggggtggtgc ttcttgagga agcggtacat
     5281 gtgcgacgag tcccccttgc cggcctccca gaaggtgtgg ctgaagagct gcgatgcggg
     5341 ggtcacgtgg ttgagcgcgt cgaaggccat catcacggcc gcgttcgtcc acgtgagctt
     5401 ctcctccggc cagacgacca tgtcggggaa ggtgaagccg caccagtagg aaccgtcgtc
     5461 gtagcgcttg tcgtgaatcc agccgaggac cctcctcgcc tcctcgtgtt cgcccatggc
     5521 ggcgagcgtc aggatgagct ccgaggtttc cgcgacggtg atccaggggt tgtccgagac
     5581 gcacctcgtg ccgagcccct ccaccatgaa cctgttccag aagcgctcga tgcggttctt
     5641 cgcctccagg cccgtgagag cgccgcagag gaccgggtag aaccagtcca tcgagtagcg
     5701 tgacttggtg cggtcgaaga ggtgcggccg gtacctgatg gcgtccccga gcctcttcag
     5761 cgcgctctgc cacctgggcc tgctctcgcc caaaaggagc gccagcacga gcgcgcactt
     5821 gaggctcatg aacaccgagc tgctcgcggt gagcaacgcc acggggtcga cctcgccggc
     5881 ggggttgcgc gcccagtaga cggcgcccgt gggcgcctgg aggccaaccg cgaagtcgat
     5941 ccctgcgctc accgtgggcc agatgtattc gaggaacgcc ttgtcgcggg tgacgaggta
     6001 gtagtgaaac acccccaccg cgatgtagct cgacatgttc gactccctcg tgaggtcctc
     6061 ggggaccccg tcccggtatg cggagtacca cgagccgtcc tcgagctgtc gggacgcgag
     6121 ccactcgaag gccttgcgcg cctcctcgta gcgtccgccg atcgtcaggc cgatggccgc
     6181 ctccacgtgg tcccacgggt cggtcttgcc gccctcgtgc caggggatct cgccggaagg
     6241 cctctgcagc gatgctatgg acgaggcgag gaggtcgagt tcgatggcag ggccctgtag
     6301 tgcctgatca cgttccatgg ctgtcgcctt tcgtgcagta gagtaccacg ctcttcgcga
     6361 tgaccgggtt gagaagccgg tcgagcgtgc gcgtgaggcg gggtttcttc atgatgtccc
     6421 agacgaggaa cttgtggtag agcctcacgg ggagggcctg gtcgttcttg tgccccacca
     6481 tgcacttgat ccaccagtag gggctgtgga gcgcgtgcgc ccaccccctg ccgatgcact
     6541 tgaggccagc ccgctcgagc agtgccacga gttccgatgt cttgtagatc ctcacgtggc
     6601 cgcccttctc ggtgtggtac tcctcggaga gagcccagca gatcctctcg ggaaggtatc
     6661 gcggcacgct caccacgagc gacttgccgg gtttcagcac ccgc
//
LOCUS       DAOSCR010000060         6667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7380_length_6667_cov_4.405929, whole genome shotgun sequence.
ACCESSION   DAOSCR010000060 DAOSCR010000000
VERSION     DAOSCR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7380_length_6667_cov_4.405929"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            768..1211
                     /locus_tag="PLT69_02670"
     CDS             768..1211
                     /locus_tag="PLT69_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ13378.1"
                     /translation="MLQSKKGFTLIELMIVVAIIGILAAIAIPAYSDYTKKSKVSEVS
                     NSLGAAMTAAQAHHSDTASWPADITNNFYTVCQNTFGVTLPTTYCAQANWTCTSATAT
                     ELVLQATLANIGTNVDGTTLILTSGADGGTRTWSGTLPQRYRPRQ"
     gene            1296..1736
                     /locus_tag="PLT69_02675"
     CDS             1296..1736
                     /locus_tag="PLT69_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ13379.1"
                     /translation="MKTTRGFTLIEVMIVVAIIGILATIGITVYDQYTKKTKVSELAD
                     ALGAAMTAAQSHHSDTATWPADISDNFYAVCQNTFGITLPTTYCVQAAWDCAVADANT
                     LSLQSTISNIGPGIDGTTLTLTSGADGGSRIWSGTIPIKYMPKN"
     gene            2371..3096
                     /locus_tag="PLT69_02680"
     CDS             2371..3096
                     /locus_tag="PLT69_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOJ13380.1"
                     /translation="MTPYRVLVSEFMLQQTQVERVQGCFQSFVDAFPSIEALAGADLK
                     EVLVRWRGLGYNRRALNVWRTAGVVVSRHGGVIPADRQTLETLPGIGPSTSGAVCAFA
                     YGIPVAFIETNIRRAFIHHFFPDRTDVRDREILPLVEKTLDRENPRDWYYALMDYGAM
                     LRKHAHNPNRRSAHYTRQAPFEGSNRQARGRVIKALLERGPMTKKELAALVGIERNRL
                     ETVLSCLEQEGFLAVTGQGVRLS"
     gene            complement(3119..3694)
                     /locus_tag="PLT69_02685"
     CDS             complement(3119..3694)
                     /locus_tag="PLT69_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13381.1"
                     /translation="MPRKERRIILICQCLVNPYCRVHILGQNFPLSREVTDYLLQRNV
                     GIIQYPCPETTAMGLMRNPQGRQQYDSVFFRTHCKELLKIPMLMVREFLKNRYRLCCF
                     IGLENSPTCGIHWGKHKVNRYGTESPMPAEEPGGKDPALRGIMAEILEEELSREGITV
                     PFLELPALSPPDSDKRRRFWQDVEAAVSPVP"
     gene            complement(4396..5187)
                     /locus_tag="PLT69_02690"
     CDS             complement(4396..5187)
                     /locus_tag="PLT69_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13382.1"
                     /translation="MKIIKALDKVRRFRQASIASDGETPVMKVYGNAPVYSESRRVDP
                     DMDTMEKNRCVCMRHDSPYLGAYKVLRTQIQKATVNHNWKSLMVTSALPGEGKTLTSI
                     NLALTFARAFDLTVLLVDCDLHRQDVHKFMGIESNNGLIDYLADGVPIKDLIIWPGID
                     KMTLISGGRAIKETTEFLTSPRMKTLVEEVKGRYQDRYVIFDTPAVLTGADAIAFASM
                     VDCIIMVVEANRTPMKDVNRALELLPKEKFLGFVLNRDNTRIDNL"
     gene            complement(5217..>6667)
                     /locus_tag="PLT69_02695"
     CDS             complement(5217..>6667)
                     /locus_tag="PLT69_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOJ13383.1"
                     /translation="VLPPVYKATTTILIEQQDVPLDYVKSAVSTYAEQQLQIINQRIM
                     STTRLMEIINRLNLYPELKSRMMTEEIIERMREDIALEPISVDVVDPKTGRPTTVTIA
                     FTISYEGKNDPGKVLQVGNVLTSLFLEENSQVRERQVTEVSNFLQDEVAKVKGDLERL
                     DGAIAQFKQLHMNNLPELVQVNMQSINDTERNIDLLNNQLTQLREKEGYLLTQLSNTP
                     QDLMQTDRQRLSELNVRLVNLKHQFSDAHPDVIKTRSEIAELEKRVDASTSKGNPRPD
                     NPAYINLAAQLASTRSEMASVNAQIESLRNRLNDYKRRIELSPQVEAEYNALLMERNS
                     TQLKYDDLMRKYMESKVSQGLEKEQKGERFTIIDPARMPEKPYKPNRLAIMLIGLVLG
                     IGAGIGTASFKEYVDSSVRNTSMLTEYTSFPVLANIPTIVLESDVAKKRDLMTRIVIG
                     LLASLVFGVLMFHFFIMDLDMVWVKLVRWMGI"
BASE COUNT         1394 a         1903 c         1903 g         1467 t
ORIGIN      
        1 gagcatgccc gcttcacggc cttgggaatg cgcccgtgtc cgcacctaag tacgggtttt
       61 tccgaggagc cgccgggctt ttcccaaggc cctgtacccg ttgcccgcgt aggtctctcg
      121 gccgtctctc gggagagacg agcaaacagg tcaccagcgg ccagtgcgat gtgagaaaag
      181 ccgatcccgt tcgggcgaag gccctttttc cgtaggttcg ctctctgctc gaacacctcc
      241 aaccaggaga gggttgcccc tcactgcgtg ggaaggcgac gtgggatacg gtgaacaagg
      301 agcggtcctg tgccgcttac agggctcgtc ctcacccgaa gcctcgcatg gaagagaggg
      361 aaggtttcaa gcgctccttt ctccagggct gccgtcctct ttgccgagac cgctctcgag
      421 agggggaaag gcaccatggc acacatcgtt atgatgaaag acgtccctcg catcgatgca
      481 cccctccttg cccctctgga tgtggatgca tctggcccct gcttcccgcc gtcacggggg
      541 cccgggtgaa aagtgtccgc actcttcatg ggatgaccca tgcctcgggt ctcgtgggtt
      601 accaaaatgt atcttgtaaa aaatttttct caagttatta ttgtccctgc cgtatatatt
      661 ggttgatgtg catgttttcc agtcaggatg ggaggatgga tacgacgccc aatcggtgcg
      721 gcttaatcat accttaagca attccacaca aaaggaggaa tgaggctatg ttacagtcca
      781 agaagggttt cacgctcatc gagttgatga tcgtcgttgc gatcatcggt atcctggccg
      841 ccattgccat cccggcatac agcgactaca ccaagaagtc gaaggtttca gaggtgtcca
      901 attccctggg agcagccatg accgcggcgc aggctcacca cagtgacact gcgagctggc
      961 ccgccgacat caccaacaac ttctacacgg tctgccagaa caccttcggc gtgacgctcc
     1021 ccaccacgta ctgtgcgcag gcgaactgga catgcacttc agccacggca accgagttgg
     1081 tccttcaggc gacgctggcc aatatcggta cgaacgtgga cggcacaacc ctgatcttga
     1141 cctccggcgc tgacggcggc actcgtacct ggagcggaac tctgccccag aggtatcggc
     1201 cgagacagta gtcgatggat ccagggggtg tgcccaggca cacccccttt cttgttagat
     1261 actgttagat aaagggattg cccgagaatg cgcatatgaa aacaaccaga ggctttaccc
     1321 tcatagaggt gatgatcgtc gttgccatta tcggcatcct tgcaaccatt ggcataacgg
     1381 tatacgacca gtataccaag aagaccaagg tttcagagtt ggccgacgcc ctgggggcgg
     1441 cgatgacggc ggcacagtct catcacagcg ataccgcgac ctggcctgca gatatctccg
     1501 ataactttta tgctgtctgt cagaacacct tcgggatcac tctcccgacg acgtactgcg
     1561 tacaagctgc atgggactgc gcggttgcag acgccaacac cctttcgtta cagtcgacga
     1621 tctcgaacat aggtccgggc attgacggga ccaccctgac cttgacatcg ggtgcggatg
     1681 gtggatcgag aatctggagc ggtactatcc ctatcaagta catgcccaag aattgacggg
     1741 ggcgaataga ggccatcctc ccgattccag gaaaggaaac cggccgtcgg ggtgagacgt
     1801 tccgacagcc tacgggggca gccggcgtta ctgccgacaa taccgcgggc tgtgggaact
     1861 cgaccatggc tggtaccctc gccaacggtc tcgacaacta tctcaacgaa gacacgctct
     1921 cctcgaggga gagacagggc gagagggccc ggtgaaatcc cgacggtacg gacacggtcg
     1981 agaggttctc cattcccaag cgggagcagc tcccaggtga aacaggacag ggaccggcgc
     2041 ctcatcgccg gtccctcttt tcttctctgg cgccgctggc caacatcccc tcccgcccct
     2101 cttggcgagt tgaaccatgc ttgtctgtcc ggctgatccc gtataccatg gtttccgacc
     2161 gggcgagctg cgcaagaagg ttgccgccga gaacaggtgt tcggcgtacg cacgcacacc
     2221 ctttgttcgg tgcgttcgag gaatataagc cagagggagg ccaacgcgca gggcataccg
     2281 gcattgcggg cattcatgta tacagctttt cttgagaagg ttttcgatca ttatgaacgt
     2341 catcgcaggg atttgccctg gcgtacggag gtgacgcctt acagggtcct cgtatccgag
     2401 ttcatgctcc agcagacgca ggttgaacgg gtgcaggggt gcttccagtc gttcgtggat
     2461 gcgtttccca gcatcgaggc cctggcgggg gctgacctga aagaggtgct cgtcaggtgg
     2521 agagggcttg ggtacaaccg cagggcgctc aatgtgtgga ggacggccgg ggtcgtggtg
     2581 tccaggcatg gcggggtgat cccagcggac aggcaaaccc tggagacgct ccccggtata
     2641 gggccgtcaa cctcgggtgc tgtctgcgcc tttgcatacg ggatcccggt cgcgttcatc
     2701 gagaccaata tccgtcgggc gttcatccac catttcttcc cggaccgtac ggatgtgcgc
     2761 gaccgcgaga tcctgcccct cgtggagaag accctggaca gggaaaaccc cagggattgg
     2821 tactatgccc tcatggatta cggcgccatg ctgagaaaac atgcgcacaa ccccaaccgc
     2881 aggagcgcac actacaccag gcaggcgccc ttcgagggct cgaaccgcca ggcgcgcggc
     2941 agggtcatca aggccctcct ggagcgggga ccgatgacga agaaggagct tgccgccctc
     3001 gtggggatcg agaggaatag gctggaaacg gtcctctcct gcctcgagca agaagggttc
     3061 ctcgccgtga ccggacaagg cgtcaggctt tcgtgagccc gagggaggca ggttgtcctc
     3121 agggaacagg agagaccgcc gcctccacat cctgccagaa ccgcctcctc ttgtctgaat
     3181 cagggggcga cagcgcaggc agttcgagga aggggaccgt gataccctcc ctggacagct
     3241 cctcctcgag gatctccgcc atgatgcccc taagcgccgg gtccttgccc cctggttctt
     3301 ccgcaggcat gggagactcg gtgccgtacc ggttcacctt gtgctttccc cagtggatgc
     3361 cgcaggtggg gctgttctcc agcccaatga agcagcagag ccggtagcgg ttcttgagga
     3421 actcccgcac catgagcatg ggaatcttga gcagctcctt gcagtgggtg cggaagaaga
     3481 cgctgtcgta ctgctggcgg ccctgggggt tccgcatgag ccccatggcc gtggtctccg
     3541 ggcaagggta ctggatgatg cccacgttcc tctgcaggag gtagtcggtc acttcgcggg
     3601 agagcgggaa gttctgcccc aggatgtgca ccctgcagta ggggttgacc aggcactggc
     3661 agatgaggat aatcctccgt tctttccttg gcaaggggag cggtcctttc tggggaaaca
     3721 ataaagacgg aagtcggcca aggcaagtgg cggggagaaa ggtccggccg cacaagggcc
     3781 gtctcgaatg cccccggcaa ggatcgaact tgcgacaccg ggattaggcc gaagttcaac
     3841 catcagaaag agtcaaagct tcagtctcag gctcgcgccg caccaggaag ccctttagga
     3901 aagcggagat cgccaagaag ctcggggcgg tcgtgaaccg ttacaagacg gccaagcact
     3961 tgcgccttac cattgaagac ggcgtcttca catgttcccg caagcagagt gtcatccacg
     4021 gggaggcttc tactctgacc atgtctctgc ttacaacgcc aaagcatctc cagcagaagg
     4081 tacccgctca cctaggctat ttcgagtcac aggaaactta ttccatccgg aaaagctcga
     4141 cacaagccat tctgcagctc gccatgatat gaaattacgg tttcattgcc atcacgcatg
     4201 cacacagcac ttccgatttc ccaaatgcca cgaccgcata gtctctctat cctccccgtg
     4261 aaaatatctc gaatatgcca acgtctggtg agcccggatg cagatccttg agtaaaaaag
     4321 cagcgccttg tacggcactg cccttcttcg ctttactgtc aaaggttctc tgtcgagacc
     4381 cccatcctcg ggatgctaga gattgtctat tctggtattg tcccggttca ggacgaatcc
     4441 gaggaatttc tctttcggga gcagctccaa cgccctatta acgtctttca tgggggtccg
     4501 gttcgcctcc accaccatga tgatgcaatc caccatggat gcgaaggcaa tggcatctgc
     4561 gccggtgagt accgcggggg tatcgaagat gacatacctg tcctggtagc ggcccttcac
     4621 ctcttccacc agggtcttca tgcggggcga ggtcaggaac tcggtggtct ccttgatcgc
     4681 tctcccgccc gagattaaag tcatcttgtc aatgccgggc catatgatga ggtccttgat
     4741 ggggacgccg tcggcaagat agtcaatcag tccgttattg ctctcgatgc ccatgaattt
     4801 atggacatcc tggcgatgta gatcgcagtc gacgagaaga acggtgaggt cgaaggctcg
     4861 ggcaaatgtg agggccaggt ttatggatgt cagggtcttg ccctcgccag gcagtgcact
     4921 tgtaaccatg agactcttcc agttatggtt cacggtagcc ttttggatct gggtcctcaa
     4981 aaccttgtaa gcccccaggt aaggggaatc gtggcgcatg cacacgcacc tgtttttctc
     5041 catggtatcc atatcggggt caacccgcct cgattccgaa tatacaggcg cattcccata
     5101 taccttcatg acaggtgtct ctccatccga tgcgatggat gcctgacgga aacgtctgac
     5161 tttgtcaagt gcctttataa tcttcatgag cagttacctc tctccttttc ccatggctat
     5221 atgcccatcc atctgaccag cttcacccac accatgtcga gatccatgat aaagaaatgg
     5281 aacatgagga cgccaaacac cagggaggcc agcagcccga tgacgatccg ggtcatgaga
     5341 tccctcttct ttgcaacatc actttccaag acgatggtcg ggatgttcgc aaggacaggg
     5401 aacgatgtgt attcggtcag catggaggtg ttgcgtaccg aggagtcgac atactccttg
     5461 aacgaggctg ttcctatccc ggcaccgatc cctagaacca gaccgatgag catgattgcg
     5521 aggcggtttg gcttgtaggg cttctcaggc atacgcgccg ggtcaatgat ggtgaaccgt
     5581 tcgcctttct gctccttctc taggccttgg gaaactttag actccatgta tttcctcatg
     5641 agatcgtcat atttcagctg tgtgctgttc cgctccataa gcagggcgtt gtattccgcc
     5701 tccacctgcg gggaaagctc aatgcggcgc ttgtagtcgt tgagcctgtt cctgaggctc
     5761 tcgatctgtg cattcacgga ggccatctca gaccgggtgc tcgcaagctg cgcggcaaga
     5821 ttgatatagg ccggattgtc aggccgggga tttcccttgc tggttgatgc atcgaccctc
     5881 ttctccagct cggctatctc ggacctggtt ttgataacat caggatgtgc atccgaaaac
     5941 tggtgcttaa ggttcacgag cctcacgttc agctcgctga gccgctgccg gtctgtctgc
     6001 atgagatcct gcggggtgtt agacaattgc gtaagaaggt acccctcctt ctccctgagc
     6061 tgggtcagct ggttgttcag cagatcaata ttccgttcgg tgtcgttgat gctctgcata
     6121 ttgacctgga ctagctcagg gagattgttc atatgcaact gtttgaattg ggcaatggct
     6181 ccgtccaacc gctccaggtc ccctttgacc tttgcgacct cgtcctggag aaagttggag
     6241 acctcggtga cctgccgttc acggacctgg gaattctcct ccaagaacag tgatgtcagg
     6301 acgttgccta cctggagcac cttgccagga tcattcttgc cctcatagga gatcgtgaat
     6361 gcgatggtca ccgtggttgg cctgcctgtt ttggggtcga ccacgtccac gctgataggt
     6421 tcgagcgcga tatcttctcg catgcgctcg atgatctcct cggtcatcat cctgctcttc
     6481 agttcagggt agaggttcag cctgttgatg atctccatga gacgcgtagt gctcataatg
     6541 cgttgattga tgatctgcaa ctgctgctcg gcataggtgg ataccgcaga tttgacgtaa
     6601 tcaagcggaa catcctgctg ctcgatgagg atcgtggtgg tcgccttgta aaccggcggc
     6661 aggacaa
//
LOCUS       DAOSCR010000061         6645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7408_length_6645_cov_3.181184, whole genome shotgun sequence.
ACCESSION   DAOSCR010000061 DAOSCR010000000
VERSION     DAOSCR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7408_length_6645_cov_3.181184"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLT69_02700"
     CDS             complement(<1..1443)
                     /locus_tag="PLT69_02700"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ13384.1"
                     /translation="MNASIRLIRILLLACLLGFSMGPSSYAASETAAVLDGESTIRPL
                     PEHGRVAVEIIRDLKGNHYRKLLIDDDLSSKVFDRLLEDIDPSRMYFTRADLGQFERY
                     RYRIDDSLLRGDVSIPFKLFNRYQKRLTGRLSFCLGLLEKGIATIDLEKDEHFETDRE
                     KARWPEDEKELEDLWRKSLKNEVISMMLEGKSAPEVQKDLSKRYSSQLKRVEQTTSED
                     AFQIAMNALTSCFDPHTTYMSPRSSENFSINMSLSLEGIGAMLTSENEYTKVVRLVPG
                     GPAEKSGQLRPNDRIVGVGQGAEGEIVDVVGWRLDDVVDLIRGPKGTPVRLKIIPASA
                     TDEHQTRIVKIMRNTVSLEDQAASKDIIEIDRDGRKYRIGVVRVPTFYLDVKAMQSHL
                     DDFKSSTRDVRRLLEELQKQGVDGVVVDLRNNGGGLLHEANTLTGLFIKTGPTVQVRS
                     ADGYTETLYDRDPTIVYQGPLATAINRLSAS"
     gene            complement(2115..2438)
                     /gene="cutA"
                     /locus_tag="PLT69_02705"
     CDS             complement(2115..2438)
                     /gene="cutA"
                     /locus_tag="PLT69_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOJ13385.1"
                     /translation="MDTGVTYCMALCTSPSEAHALELARGLVDHRLAACVQVLPIRSV
                     YRWEGKREEEEERLILAKTRVDLFDRLVEFVRKGHPYQVPEIVRLDIAGGLDAYLSWV
                     GESTG"
     gene            complement(2449..2823)
                     /locus_tag="PLT69_02710"
     CDS             complement(2449..2823)
                     /locus_tag="PLT69_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOJ13386.1"
                     /translation="MEKVVIRCGGIRVEAGLTEGPTARKVASLLPIEGRARRWGDEIY
                     FTVPIDAGLEPGATSDVEAGDIAYWPDGPALCIFFGPTPSSTGEKPRAYSPVTVIGRA
                     QGDPTVFRTVRDGDTITIERGE"
     gene            3175..4185
                     /locus_tag="PLT69_02715"
     CDS             3175..4185
                     /locus_tag="PLT69_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13387.1"
                     /translation="MKITDSIVTADAARSYSEYHHQRTEFRFWVDRPSIQTQDRVNIS
                     AEAYACPPGREEEGALGDECEPAVTIERLITEVLSGKKIRVLRLEELRGDNPDAAPGD
                     AGDLDGGPSASQGWGLDYSHTEEYAERERVTFDARGVVTTSDGRRLAFTLHLEMDREY
                     VERSEVRIRAGDALKDPLVINLDGSAVSLAGSRYAFDLDGDGVEESIPGLAPGKGYLA
                     LDLDANGVIDDGRELFGPSTGDGFSELSALDEDKNGWIDEADKAFGMLRVMIFDGSGA
                     RVVSSLADVGVGALSTGSLPTPFDMKDPVTGERHGRVRATGLYIAENGAVGTLQQLDL
                     VV"
     gene            4538..4999
                     /locus_tag="PLT69_02720"
     CDS             4538..4999
                     /locus_tag="PLT69_02720"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HOJ13388.1"
                     /translation="MTYDVDIEERFREGARFLEQAVGIIAKTGIRIVSLRERYARIML
                     PFEPNVNHIGTMYGGSLFILAEFSGGVIYYVSFDHSRFYPIVKEVNIRYRAPALTDCF
                     LEASLSEEEVGRIQSLADMNGKQDWVMDLDIKDTQGKTCCQVKGTWQLRRF"
     gene            complement(5481..>6645)
                     /locus_tag="PLT69_02725"
     CDS             complement(5481..>6645)
                     /locus_tag="PLT69_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13389.1"
                     /translation="TPGMFTFEEVIRAGGKVTDEELAAREREVHFHDVVNMQYTSGTT
                     GFPKGVMLTHYNIINNARMVGDVMGMTPSDRLLIQVPLFHCFGCVMSTLNCVVHGSTM
                     VIVEAFDPLKALKYIEQEKCTAVNGVPTMFIAILSHEDFAKYSTKSLRTGIMAGAPCP
                     VDTMNQVREVMHCPEIVIAFGQTECSPVMTMTRRDDPVELRVSTVGRLLPGIEGKIVD
                     PSTGEELPPDVQGEIVTRSPCVMKGYYKMPEETAKAIDKDGWLHTGDLGTVDANGYFK
                     VTGRIKEMIIRGGENIYPREIEEFLRKNPKVKDVYVVGIPDKKYGEQVLAAVMLKPGE
                     RATAEEFTAFCQGRIARHKIPRYWEFVDSVPMTASGKVQKYKMVEEYSKKYAA"
BASE COUNT         1072 a         2116 c         2211 g         1246 t
ORIGIN      
        1 ggaggcgctc aagcggttta tggcggtggc cagcgggccc tggtagacga tcgtggggtc
       61 gcggtcgtag agggtctcgg tatagccgtc cgcggacctg acctgcacgg tggggccggt
      121 cttgatgaac agccccgtga gggtgttggc ctcgtggagc agccccccgc cgttgttcct
      181 caggtccacg acgacgccgt cgacgccctg cttctggagc tcctcgagga gccgcctcac
      241 gtcgcgggtt gagctcttga agtcgtcgag gtgcgactgc atggccttca cgtcgaggta
      301 gaaggtgggc acgcgtacga caccgatgcg gtacttcctc ccgtcccggt cgatctcgat
      361 gatgtccttg ctcgcggcct ggtcctcgag gctcaccgtg tttctcatga tcttgacgat
      421 cctcgtctgg tgctcgtccg tggcggacgc aggtatgatc ttgagcctca cgggcgtccc
      481 cttgggcccc ctgatgaggt cgaccacgtc gtcgagcctc caccccacga catccacgat
      541 ctccccctcg gcgccctgcc cgacgcccac gatcctgtcg ttcggccgga gctggccaga
      601 cttctccgca gggccgccgg gcacgagcct gaccaccttc gtgtactcgt tctccgaggt
      661 gagcatggcg ccgatgccct cgagggaaag gctcatgttg atggaaaagt tctccgagga
      721 gcgcggcgac atgtaggtgg tgtgcgggtc gaagcacgag gtgagggcgt tcatggcgat
      781 ctggaaggca tcttcgctcg tggtctgctc gaccctcttg agctggctcg agtagcgctt
      841 ggaaaggtcc ttctggacct ccggcgcgct cttgccctcg agcatcatgg agatcacctc
      901 gttcttgagg gactttcgcc agaggtcttc gagttccttc tcgtcctcgg gccaccgggc
      961 cttctcacgg tccgtctcga agtgctcgtc tttctcgagg tcgatggttg cgatgccctt
     1021 ctccagcagg cccaggcaga acgagagcct cccggtgagc ctcttctggt agcggttgaa
     1081 gagcttgaac gggatggaga cgtcccccct gagcaggctg tcgtcgatgc ggtaccggta
     1141 ccgctcgaac tggccgagat ccgccctggt gaagtacatc cgcgacgggt cgatatcctc
     1201 gagcaggcgg tcaaacacct tcgaggagag gtcgtcgtct atgagcagct tcctgtagtg
     1261 gttgcccttg aggtcccgga tgatctcgac cgcgaccctg ccgtgctcgg gcaggggcct
     1321 gatcgtactc tccccatcca gcaccgcggc ggtctcggag gccgcgtacg agctgggacc
     1381 catggagaac ccgagcaggc atgcgagcaa caggatccgt atgagtcgta tcgaggcgtt
     1441 catcggtcgt gtgcgtgccc tcccggtgtc ttgcccttat aacaagtgcg cgtcgaaaac
     1501 acaatgccgc tggtgatccc ggccggcgca tgcccgtgac gggaacgggg aagggcacgg
     1561 gaatacgcag gaagcactca tggggaaacc agccgcaccc ggcacctaag acgccggggc
     1621 cggtctggga aagggtgacc gcaccgcttc gtgcctgcat gcgtgccgca tgcctcgagg
     1681 cccgggcggg cttgccgagg ggatgggtgg ggatcgcggc acgggaacgc atgagcgccg
     1741 gggatgggcc atcggaggtg cgtggaaagg ggtgtatgcg ccgagtcgag acggcccatc
     1801 gctcctgagc ggtcgggcct gggactgtcc tgccggcaag cgctcaccgc ccccgtgacc
     1861 caaggcgaga ccaccgggaa agcgtctcct cgacctttta ttggcttcgt caacccgaga
     1921 acgcggcgtc tcccaagctt tcaggggtgc cgcttcccgg gtgcggccga gaaagccgtg
     1981 gtcgccgctt ctttcaagcg ttcaggggtg acgcccatgt ccgtgaggtg cggtatggcc
     2041 tcccatgcga tcccgcgggt caggggcgtc tcgaccacgg ggaggatacg cctgctttgc
     2101 cgtatgcagc ctcctcaacc ggtcgactcg cctacccacg agagataggc gtccaggcca
     2161 cccgcgatgt ccagcctcac gatctcgggc acctggtagg ggtggccttt cctgacgaac
     2221 tcgacgagcc tgtcgaacag gtcgaccctg gtctttgcga ggatgaggcg ttcctcctcc
     2281 tcttctctct tcccctccca ccggtacacg ctcctgatcg gcaggacctg gacgcacgcg
     2341 gcgagcctgt gatccacgag gccgcgggcg agctcgagcg cgtgcgcctc gctcgggctc
     2401 gtgcagaggg ccatgcagta cgtcacgccg gtgtccatgg catccccctc actcccctct
     2461 ctcgatcgtg atggtgtccc cgtccctgac ggtcctgaag accgtcgggt cgccttgtgc
     2521 ccggcctatc acggtcaccg ggctgtaggc ccggggcttc tcgcccgtgc tggaaggcgt
     2581 gggcccgaag aagatgcaga gggcgggtcc gtccggccag taggcgatgt cccccgcctc
     2641 gacgtcggat gtggccccgg gttccaggcc cgcatcaatg gggacggtga aatagatctc
     2701 gtcgccccat cgccgggctc tgccctcgat ggggaggagg gatgccacct tccgggcggt
     2761 gggcccctct gtgagccctg cctcgacccg gataccaccg catctgatga ccaccttctc
     2821 catgcaggac cacctcccgt gaagactccg gcacggcatc acccgaagag ccggtgcccg
     2881 tgacgtaggt atgcatgcat acagccggcc gtgtcaacct gctgaagtcc tagaagtgcg
     2941 ccccggccgc acccctctcg tcatgcgctc gaggccttcg cgctcttgtg tgcatgcacc
     3001 gggtgcagcg tttaggtgca ggggtgtgcc cgccggatgt tccagggtat tcatcccctg
     3061 aagccgcaag ggaggcggca ggtgcctctc gttctttgcg ggacgcggac gcttcaggtt
     3121 tttccgcttt gtgccgagaa ggaaggtaag gcttttcagg gacgaggagc ttgcatgaag
     3181 atcacggact cgatcgtcac ggctgatgcc gcgcgttcct attccgaata ccaccatcag
     3241 cgtaccgagt tcaggttctg ggtggaccgg ccgagcattc agacgcagga cagggtgaac
     3301 atatcggccg aggcgtacgc gtgtcccccg gggcgggaag aggagggtgc actcggcgac
     3361 gaatgcgaac ccgccgtgac catcgaacgg ctgatcaccg aggtgctctc ggggaaaaag
     3421 atacgggtgc tgcgcctcga ggagcttcgc ggcgacaacc cggatgcggc gccgggtgat
     3481 gcgggcgact tggacggcgg ccccagcgca tcacaggggt ggggcctcga ctactcgcac
     3541 accgaggagt acgcggagag ggagagggtc accttcgacg cgcgcggggt ggtcacgacc
     3601 tctgacggga gacggctcgc cttcacgctc cacctggaga tggacaggga gtacgtggag
     3661 aggagcgagg tgcgcatacg cgccggcgac gccctgaagg acccgctggt catcaacctc
     3721 gacgggtccg ccgtgagtct tgcggggtcg aggtacgcct tcgacctcga cggggacggt
     3781 gtggaggagt cgatccccgg tcttgcgccg ggcaaggggt acctcgcgct ggacctcgac
     3841 gcgaacggcg tgatcgacga cgggagggag ctcttcggcc cgtccaccgg ggacgggttc
     3901 tccgagttgt cagccctgga cgaagacaag aacgggtgga tcgacgaggc cgacaaggcg
     3961 ttcgggatgt tgagggtcat gatcttcgac ggctcgggcg ccagggtggt gagcagcctt
     4021 gcggacgtgg gcgtgggcgc cctgtcgacc gggtcgctcc cgaccccctt cgacatgaag
     4081 gaccctgtca ccggcgagcg ccacggccgc gtgagggcga ccggcttgta catcgcggag
     4141 aacggcgcag tcggcacgct ccagcaactc gacctcgtcg tctagcgtac gccggctcga
     4201 agcgccattc tcacgctgtt actgccaagc gcccgagcaa gcatggccgc cgttaagcat
     4261 aggccggctc gtcgcgcctt tctcgccgaa cccgcacaca gggctccttt gcccccacgg
     4321 ccttcacgcc acacctcagg cgtcggtcac gacacatgcg gggtacagaa agacccccga
     4381 ccgccaggcg gcctacggat ggaagatgta cccgtgtacc tcgcaggtac aggcagccac
     4441 atcgaccgcc agggatcgtc tttcctgcct gtttggaggt gttgtgccgg ctcccgccat
     4501 gggttatgct acgggtatct catgaggggg cctcacgatg acctacgacg ttgatatcga
     4561 ggagaggttt cgagaagggg cgcgttttct ggaacaggcc gtgggcatca tcgccaagac
     4621 aggcatcagg atcgtctcct tgagggagcg ctacgcgagg atcatgcttc ccttcgaacc
     4681 gaacgtgaac cacatcggga cgatgtacgg cggctcgctc ttcatcctcg cggagttctc
     4741 gggcggtgtg atctattacg tctcttttga ccactcgagg ttctacccca tcgtcaagga
     4801 ggtgaacatc cgctacaggg cgcctgcgct cacggactgt ttcctcgagg cgagcctgtc
     4861 cgaggaggag gtgggccgta tccagtcgct cgccgatatg aacggcaagc aggactgggt
     4921 catggacctg gacatcaagg acacccaggg aaagacctgc tgccaggtga aaggcacctg
     4981 gcagctgagg agattctagc cgtacgcttc tgtgcgcctg atcccgctcg cgcgcgcttg
     5041 tgccggacgc ccgcctgtcg aagggggctc tcgggcggca agcaggagac caactgtaca
     5101 gctcgagcct ctcccacggg acgcccgccg gctgaaggga atctccctcg gctttcccgc
     5161 gcctgtatgc gcgtgcaccc cgtgtggggc gtttggcggc cggcaaggtt ccagaccggc
     5221 ggaagccgtc acgacggtca tgagggcctc tagggacagg aaagggctat ccgcctaggc
     5281 agggggtcgc catctgccga gtcgcccggg gcatggggct gtggggcaac cccgattcgg
     5341 ggtgggtgag gaccgaggca gggcaaggct cctgcacaca agccccaggg cgggcgccgc
     5401 ggcgagcggc cgcgagggcg gcctgccgcg gcgggctcgg gcggcctgga gggactgcag
     5461 cccccggtgc gctcgagcga tcaggcggcg tacttcttcg agtactcctc caccatcttg
     5521 tacttctgca ccttgccact tgccgtcatg ggcacggaat ccacgaactc ccagtacctg
     5581 gggatcttgt ggcgggcgat cctgccctgg cagaacgcgg tgaactcctc ggcggtggcc
     5641 ctttctccgg gcttgagcat caccgccgcg agcacctgct ccccgtactt cttgtccggg
     5701 atccccacga cgtagacgtc cttgaccttc gggttcttgc ggaggaactc ctcgatctcc
     5761 cgcgggtaga tgttctcccc gcccctgatg atcatttcct tgatgcggcc cgtgaccttg
     5821 aagtagccgt tggcgtcgac ggtgccgagg tcgcccgtgt gcagccagcc gtccttgtcg
     5881 atggccttgg ccgtctcctc gggcatcttg tagtagccct tcatgacgca cgggctcctg
     5941 gtgacgatct cgccctggac atccggcggg agctcctctc ccgtggacgg gtccacgatc
     6001 ttgccctcga tcccgggcag cagcctaccc acggttgaca ccctgagctc gaccgggtcg
     6061 tcgcggcggg tcatggtcat gacgggggag cactcggtct ggccgaaggc gatcacgatc
     6121 tctgggcagt gcatgacctc gcgcacctgg ttcatggtgt ccacggggca gggcgcgccg
     6181 gccatgatgc cggtcctcag cgacttcgtc gagtatttgg cgaagtcctc gtggctgagt
     6241 atcgctatga acatggtggg gacgccgttt accgccgtgc acttctcctg ctcgatgtac
     6301 ttgagcgcct tcagcgggtc gaaggcctcg acgatgacca tggtggagcc gtgaaccacg
     6361 cagttcagcg tgctcatcac gcacccgaag cagtggaaca gcggcacctg gatgagcagc
     6421 cggtcggaag gggtcatgcc catgacgtcc cccaccatcc tggcgttgtt gatgatgttg
     6481 tagtgggtga gcatcacgcc cttggggaag cccgtggtcc ccgaggtgta ctgcatgttc
     6541 acgacgtcgt ggaagtggac ctcccgctct ctggctgcca gttcctcgtc ggtgaccttg
     6601 ccgcccgcgc ggatgacctc ttcgaaggtg aacatgcccg gggtg
//
LOCUS       DAOSCR010000062         6628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7430_length_6628_cov_3.828997, whole genome shotgun sequence.
ACCESSION   DAOSCR010000062 DAOSCR010000000
VERSION     DAOSCR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7430_length_6628_cov_3.828997"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            513..1439
                     /gene="mtgA"
                     /locus_tag="PLT69_02730"
     CDS             513..1439
                     /gene="mtgA"
                     /locus_tag="PLT69_02730"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOJ13390.1"
                     /translation="MTTRLFKLVLAAFLITILYILSAFVYPDVGALAKRHPEKSAFME
                     YRQRQWRRQGVDVKIRHTYVPLRAISPYVVKAVIIAEDDKFWRHEGFDFEAMQNALEK
                     DIRERRLKAGGSTISQQLAKNLFLSPSKNPVRKIREAIYTWRLERTLKKRRIVELYLN
                     YAEWGPGIFGIEAASRRYFGKPASSITPMEAARLAAVLPNPIRYSPTGSSRFVENRAR
                     RIYRIMLARGIVIPEYVEVMSAPQEPEPLAEPTLEDTQGAASPDRSTEEPGLDSPSEQ
                     DAARDGEPEGGEAPGDPREGPPDAGDQGEPPR"
     gene            1902..3461
                     /locus_tag="PLT69_02735"
     CDS             1902..3461
                     /locus_tag="PLT69_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOJ13391.1"
                     /translation="MSHHFPLRAFPSTSTSVRAMAEQAFSRAAGAQLVEGNSIRLLKD
                     ATQNYPAWIDAIASASLWVHFENYFITDDAAGRMFAEVMADKARQGVKVRLVYDWMGS
                     FGRASGRFWDTLRKAGVDVRCFNPPRLDEPFGWVVRNHRKSLSVDGRIGFVSGLCIGS
                     AWQGDPSRAIEPWRDTGVAITGPAVADIDQAFADVWATMGPPLPDEEIPDPASIHPTG
                     EVTLQVIATAPYTTGLYRLDQLVAALARSTLWLTDAYFLGLPPYVQALKAAAMDGVDV
                     RLLVPGTTDIPVVRALSRAGYQTLLEAGVRVFEWNGPMLHAKTAVADGRWARIGSSNL
                     NVSSWVGNYELDVAVENEAFAQAMERMYVEDLSRSTEVVLSGGRKVCLAGRRPTRRRL
                     GGAGIRGRRRGSLGKAGVGVMRLGNVVEAAISGRRALGSVETTITLASGVVLLALAAA
                     VLVWPKLLAVPFAVVCLWLSWALFLRAWRLGRRRARLDDALSGKSPGEQCPSSRDHAP
                     RGGDAGPLARE"
     gene            complement(3508..4455)
                     /locus_tag="PLT69_02740"
     CDS             complement(3508..4455)
                     /locus_tag="PLT69_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOJ13392.1"
                     /translation="MTFLFFWVHTALWWRKSYWEKHRREKEGIVEEGCLITEGGHVHV
                     ERFSRSERLMHLVLICSFFTLVTTGFPLKYHREPWAPLFLDFWGGAHMAGVAHRIAAA
                     VLILLFSIVAWRSVRFVVAPGAGWKGWKERLFGPDSLFPRWKDWEDLKGMVLWFFDRG
                     EKPRFDRWTYWEKFDFWAVFWGMFAIGGSGVLLWSPELSSYIVPGWVLNVASLVHSEE
                     ALLAALFIFTVHFFNTHFVPDKFPMDRLIFTGTYSIDELKRHRPQEYERLASQGKLDE
                     LKRPHPGIPLKLAAAVVGLASLLFGSLLTVLFIVALVGR"
     gene            complement(4452..5330)
                     /locus_tag="PLT69_02745"
     CDS             complement(4452..5330)
                     /locus_tag="PLT69_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOJ13393.1"
                     /translation="MSRARTCLAFFLVVASAWAVEAGIFRVDLWAQQDQGCERCHYDQ
                     TYTTGFAASVHARNSCASCHGFIKDLGSHMRGADRPKPVDCTTCHERISREYLQNFHY
                     LQEDFRCVDCHGDIHTRTASKVPVKIRAVKACTRCHLNEEYVASGHAEALLRGNMDAA
                     SCMDCHGLHDTRVYHTALRTYPEEARMFYNTRCIACHSDAAMMGRNALSAKIVEYYEE
                     TYHGKVQDVGFATLVAGCADCHTTHNILPKTDPRSSIHPDNLVTNCGRCHTGFHPRFV
                     LYEAHPDYRDRPRSWS"
     gene            complement(5327..6070)
                     /locus_tag="PLT69_02750"
     CDS             complement(5327..6070)
                     /locus_tag="PLT69_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOJ13394.1"
                     /translation="MNRFLGTPSTVLLALALFCLVQAQPAQSRQGWRFVSPDTLEGWM
                     RTDQVTLVNVMSRIECLDHRIARSVCIACEQFASSMETLRKDVKLVLYCESEMCHRSC
                     LAADEAVKAGFREVYVLNGGLPAWKRAGYATESVQRVKRRPIPAVKAEELKRWLMRHG
                     QYAVLDIRSREQYRREHLDGAVNIPFSELHERYHELARDRGYLVVDDQGFRSFLASCY
                     LAEKGFQVVRLYGGMAKWRSYKQAQEKRR"
     gene            complement(6063..>6628)
                     /locus_tag="PLT69_02755"
     CDS             complement(6063..>6628)
                     /locus_tag="PLT69_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13395.1"
                     /translation="VNLGKGYGERYVPVSLTTAQWFAAQGPGGALEPLDGGVVARAAK
                     SVVGLGTVEVVTVGGGKTRIRTVAEDKDIAAMIRALGAPGGIRPVFISDSVYELEGGR
                     LAARALPFPNTVVRPVWHGVSPVGVGRTYGASGCTDCHSPRSVFFTAWRIRSVGKFIK
                     VDGFEHKPPNSFPQMTLWGFDEVPSHE"
BASE COUNT         1235 a         2206 c         2068 g         1119 t
ORIGIN      
        1 ctggcagccc tcatacgctg acacccggag cggacaatcc tctcccgggc acaccgccgg
       61 cgcacctgag cccacccgac ctgcccctct cgatccccga tctcggacgc cacctcagcg
      121 gagagacgag acccgggcat ggccgtgcct gacaacacgg ctgcacgtgc actcaggccc
      181 ccgaggggtg gagggacgtc gatgccgagc ccgccctctt gcaccgagct cggcatacat
      241 ccggtgcgat ggggaccggg ccgccgccgg gcacccgggg atcgtggcgg gaagggcctt
      301 cgagctcccc ttggccgcgc cccgccgacg tcatcgaacc cccggggcgt gcaggcagag
      361 gcagtgaaca gacccaaagg ggcatgcacg tggatgtaca gggggcccca tggcgagcct
      421 cctcgaaaga tttccttgtc atgggagcag gccatgatct atgactcgta aggtccggca
      481 ggccgagacc ctgcctcggg gagctgtgtt tcatgacgac cagactgttc aagctcgtcc
      541 tcgcggcctt tctcatcacc atcctctata tcctttccgc cttcgtgtac cctgatgtgg
      601 gagccctcgc caagagacac cccgaaaaga gcgccttcat ggaatacagg caacgccagt
      661 ggcggcgcca gggcgtggac gtgaagatca ggcacaccta cgtgcccctg agggccatat
      721 cgccctatgt cgtcaaggcg gtgatcatcg ccgaagacga caagttctgg agacacgagg
      781 ggttcgactt cgaggccatg cagaacgccc tcgagaagga tatccgggaa cgcaggctca
      841 aggcaggggg gtcgaccata agccagcagc tcgcaaagaa cctcttcctg tcgccttcca
      901 agaacccggt acggaagata cgggaggcca tatacacctg gcgcctggaa cgcaccctca
      961 agaagcggcg catcgtggag ctctacctca attacgcaga gtgggggccg ggcatcttcg
     1021 ggatcgaggc cgcatcgaga aggtatttcg ggaaaccggc ctcgagcata acgccgatgg
     1081 aggcggcacg cctcgcagcg gtgcttccga atccgatcag atacagcccc accgggagct
     1141 cgcgcttcgt ggagaacagg gcccggagga tatacaggat catgctcgcg cggggtatcg
     1201 tgatccccga gtacgtggag gtcatgtccg caccccaaga accggagccc cttgccgagc
     1261 ccaccctgga agacacccag ggagcggcct ctcccgaccg gtcgacggag gaaccagggc
     1321 tcgacagccc aagcgaacag gatgcagccc gagacggaga gcccgaaggc ggggaagcgc
     1381 ccggcgaccc acgggaagga ccgccggatg ccggggatca aggcgagccc cccaggtgaa
     1441 ccctgcccat cgaaaaacgg gtgggggaaa tacaccggac gatcacggtt gatcgacgcc
     1501 ccgaagctga accctgtgca tgggctcgtg aggggaccac gttgtcggac ccccagggaa
     1561 accccttcgg ggtgacgatg gccccatggc gtggttcgaa aggggaggcg gcatggggca
     1621 tgaggtgagg catcgacacc cgcttttaac gctctcgtcc gcctcggtct tcccgctcag
     1681 ggctctcgag caggccttga tccccctctt cgcaggtcac gatctcgtat ccacttcgtc
     1741 ggcatggaga acacgtgttc ataccacccc ttcatcaccc catcgagccg cgcgcgacag
     1801 gtcgcatcca tgcagctggc cgagacgcgc cttcggcccc gggatccctc gccgtttgaa
     1861 cctgtagacg cgacctcgcc ggtgctggta gactcgccac catgagccat cactttcccc
     1921 tgcgcgcctt cccctcgacg agcacctcgg tgagggccat ggccgagcag gccttctcgc
     1981 gcgcggcggg cgcgcaactc gtcgagggca acagcatccg cctcctcaag gacgcgacgc
     2041 agaactaccc tgcatggatc gatgccattg catcggcatc cctgtgggtt cacttcgaga
     2101 actacttcat cacggacgac gccgcgggca ggatgttcgc ggaggtcatg gccgacaagg
     2161 caaggcaggg ggtgaaggtc cgcctcgtct acgactggat gggatcgttc ggaagggctt
     2221 ccggcaggtt ctgggacacc ctcagaaagg cgggcgtcga tgtccggtgt ttcaacccgc
     2281 cgcgcctgga cgaacccttc ggctgggtcg tccgcaacca ccgcaagtcg ctctccgtcg
     2341 acggccgcat agggttcgtc tcgggcctgt gcatcggcag cgcatggcag ggtgacccgt
     2401 ctcgagccat agagccatgg agggacaccg gcgtcgccat aaccggcccg gcggtcgcgg
     2461 acatcgacca ggccttcgcc gacgtgtggg cgaccatggg gccgcccctt cccgacgagg
     2521 agatccccga cccggcgtcc atccacccca cgggagaggt caccctgcag gtcatcgcca
     2581 ccgcaccgta caccacggga ctctacaggc tggaccagct cgtggcggct cttgcgcgct
     2641 cgaccctgtg gctgaccgac gcctacttcc tgggcctgcc cccttacgtc caggcgctca
     2701 aggccgcggc catggacggt gtggacgtga ggcttctcgt ccccggcacc acggacatcc
     2761 cagtcgttcg ggccctctcc cgggcggggt accagacact cctggaggcg ggggtgcgcg
     2821 tgttcgagtg gaacggcccc atgctccacg cgaagaccgc cgttgcggac ggccggtggg
     2881 cgaggatcgg gtcgtcgaac ctcaacgtgt cgagctgggt gggcaactac gagctcgatg
     2941 tcgcggtcga gaacgaggcc ttcgcccagg cgatggagcg catgtacgtg gaggacctgt
     3001 cgcgatccac cgaggtggtt ctctccgggg gcaggaaagt ctgcctggca ggccgacggc
     3061 ccacccgccg gaggctgggc ggagcgggca tcaggggcag gcgacggggg tccctaggca
     3121 aggcaggcgt tggggtcatg aggctcggca atgtcgtgga ggcggcgatc agcgggagac
     3181 gcgccctggg atcggtggag acgaccatca ccctcgcctc cggagttgtg ctcctggccc
     3241 tggcggccgc cgtcctcgtc tggccgaaac ttctggcagt acccttcgcc gtggtgtgcc
     3301 tgtggctctc ttgggccttg ttcctgaggg catggcgcct cgggaggaga cgtgcacgcc
     3361 ttgacgacgc cttgtcgggc aagagccccg gtgaacagtg cccctcttct cgcgatcacg
     3421 caccgcgcgg aggcgacgcg ggccctctcg caagggaatg acgcatgcaa ccgcaacccc
     3481 gaagccacag cggcagcgcc atgccgctca ccgtccgaca agggccacga tgaacaggac
     3541 cgtgagcagc gacccgaaaa gcaggctcgc cagcccgacg acggcggccg ccagcttgag
     3601 ggggatgccg gggtggggcc tcttgagctc gtccagcttt ccctgggacg caagcctctc
     3661 gtactcctgg ggcctgtgcc tcttgagctc gtcgatggag taggtgcccg tgaatatgag
     3721 gcggtccatg gggaacttgt ccggcacgaa gtgcgtgttg aagaaatgga ccgtgaagat
     3781 gaacagggct gcaaggagcg cctcctcgga gtggacgagg gacgcgacgt tgagcaccca
     3841 cccgggcacg atgtaggaag agagctcggg gctccacagc aggacgcccg agcccccgat
     3901 ggcgaacatc ccccagaaga cggcccagaa atcgaacttt tcccagtatg tccatctatc
     3961 gaacctgggc ttctcccctc gatcgaaaaa ccacagcacc atgcccttca ggtcctccca
     4021 gtccttccat ctggggaaca gcgagtcagg gccgaaaagg cgttccttcc accctttcca
     4081 tcccgcacct ggagcgacca cgaaccggac ggagcgccag gccacgatgg agaagagcag
     4141 gatgaggacc gccgccgcga tgcggtgggc tacgcccgcc atgtgggcac ccccccagaa
     4201 atcgaggaag aggggcgccc atggttcccg gtggtacttc aaagggaacc ccgtggtgac
     4261 gagggtaaag aaggagcaga tgaggaccag gtgcatgagg cgctcggacc tcgaaaaccg
     4321 ctccacgtgg acatgtccgc cttcggtgat caagcaaccc tcctccacga tgccttcctt
     4381 ctcgcgcctg tgcttttccc agtagctctt tctccaccag agcgccgtgt ggacccagaa
     4441 gaacaagaac gtcacgacca agagcgagga cggtctctgt agtcggggtg agcctcgtag
     4501 agcacgaaac gcgggtggaa ccccgtatgg cacctgccgc agttggtcac gaggttgtcg
     4561 gggtgaatgc tggaccttgg gtcggtcttg ggaaggatgt tgtgggtggt gtggcaatcc
     4621 gcgcacccgg ccaccagggt ggcgaacccg acgtcctgca ccttgccgtg ataggtttcc
     4681 tcgtaatact cgacgatctt cgccgagagg gcgttgcgtc ccatcatggc cgcatcgctg
     4741 tggcaggcta tgcacctggt gttgtagaac atgcgggctt cttccgggta cgtccggaga
     4801 gccgtgtggt agacgcgcgt gtcgtgcagg ccgtgacagt ccatgcacga ggcggcgtcc
     4861 atgttccccc tgagcaaagc ctctgcgtga ccggacgcca cgtactcctc gttgagatgg
     4921 catctcgtgc atgccttcac ggcccggatc ttcaccggca ccttcgacgc cgttctcgtg
     4981 tggatgtctc cgtgacagtc aacgcacctg aaatcctcct gcaggtagtg aaagttctgc
     5041 aggtattccc gggagatcct ctcgtggcag gtcgtgcagt cgaccggttt cggtctgtct
     5101 gcgcctctca tgtgggagcc gagatctttg atgaacccat ggcagctggc gcatgagttc
     5161 cgtgcgtgca ccgaggcggc gaagcccgtc gtgtaggtct gatcgtagtg gcagcgttca
     5221 cacccctggt cctgctgcgc ccacagatcc acccggaaga tcccggcctc gaccgcccag
     5281 gccgaggcca cgaccaggaa aaaagccagg catgtccttg ctcggctcat ctcctcttct
     5341 cctgcgcctg cttgtatgaa cgccacttgg ccatcccgcc gtacagcctg accacctgga
     5401 agcctttctc agccaaataa cacgatgcca aaaaggatct gaacccttgg tcgtccacca
     5461 cgaggtaccc ccggtccctc gcgagctcgt gatagcgttc gtggagttcg gaaaacggga
     5521 tgttcaccgc accgtcaagg tgttcccgcc ggtactgttc tctcgaccgt atgtccagca
     5581 ccgcgtactg gccgtgccgc atgagccacc gcttgagctc ctccgccttg acggccggga
     5641 tgggtcgtcg cttcaccctc tgcaccgact ccgtggcgta tcccgccctc ttccacgcgg
     5701 gcagaccgcc gttgagaaca tagacctccc tgaacccggc cttcaccgcc tcgtccgcgg
     5761 cgaggcagct tctgtggcac atctcgcttt cgcaatagag gaccagcttc acatccttgc
     5821 ggagagtctc catggagctt gcaaactgct cgcacgcgat gcagaccgaa cgagcgatac
     5881 ggtgatccag gcactcgatg cggctcatca cgttcaccaa ggttacctga tccgtcctca
     5941 tccacccctc gagcgtgtcc ggggacacga acctccaccc ctgacgtgat tgggcaggct
     6001 gcgcctgcac caggcagaaa agggcgaggg ccaacaggac cgtgctcggc gttcccagga
     6061 acctattcat gggaggggac ctcgtcgaac ccccacaagg tcatctgggg gaaagaattg
     6121 ggcggcttgt gttcgaaccc gtccaccttt atgaatttcc ccaccgatcg tatcctccag
     6181 gcggtgaaaa acaccgagcg cgggctatgg caatccgtgc accccgacgc gccgtatgtc
     6241 cgacctaccc ccacaggtga caccccgtgc cagacgggac ggacgacggt attcgggaag
     6301 gggagggctc tcgccgcaag acgcccgccc tcgagctcgt agaccgagtc ggagatgaac
     6361 acgggtcgta taccccccgg tgcgccgagc gccctgatca tcgcagcgat atccttgtct
     6421 tccgcaaccg tacgtattcg cgtcttcccc ccgcccaccg tgaccacctc gaccgtgccg
     6481 aggcccacca ccgactttgc cgccctggcc acgaccccgc catccaaggg ttcgagcgcg
     6541 ccccccgggc cttgtgcggc aaaccactgg gccgtcgtca aggagacggg gacgtacctt
     6601 tctccgtatc ccttgccgag gttcaccc
//
LOCUS       DAOSCR010000063         6613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7449_length_6613_cov_3.515706, whole genome shotgun sequence.
ACCESSION   DAOSCR010000063 DAOSCR010000000
VERSION     DAOSCR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7449_length_6613_cov_3.515706"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..2294
                     /locus_tag="PLT69_02760"
     CDS             57..2294
                     /locus_tag="PLT69_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ13396.1"
                     /translation="MGEWKKTGCVLCAQNCGLEVYVENNRIVKARGDRDNPRSKGYLC
                     RKGANIAFHQHHADRISSPLKRVGDRFERISWDQAIGEIAERLKAIVETHGPRSLAYM
                     GGGGQGCHFEAAFGVRLMRALGSRYHYNALAQELTGIFWASGRLAGRQYLYAIPHEEE
                     ADMLVAVGWNGMQSHQMPRAPLVLREFSKNPDKLLVVIDPRRSETAEIADIHLPVRPG
                     TDALLARAMISIILEEGWQDREYLELHVQGFEEIEPWFRGFDARDAVRVCELDYDAVR
                     GLCRLMTTRRWCMHTDLGVYMNRHSTATTYLLLLLLAVCGRLCVPGGNVIPGHLMPIG
                     AHTDERDPKNWKTVTTGFPALMGVYPPNVMPEEILSDHPERLRAVLCCQSNPLRSFAD
                     TTMYEKAFGRLDLLVTCELSMTETARLSHYVLPARSGYESWDGTFFAWTFPEIYFQMR
                     RPVVEPEGERLELSSIHTMLAEKLGIVPPIPDSLVEAASKDRLTFGAELMNYATAEPR
                     ALANMPFILAKTLGERLGSPNLAALWGLLQTAPKSFRKAAARAGFTPGITMGEEIFQA
                     ILDHPEGLILGLADKEDNFSMIAHEDKKLHVSIPEMDPWVREIEPGREEEALAPHPDY
                     PLILQAGRHMSANANTLMRDPAWNEGRRACTVSVHPEDAGRIGISDGQIVRVITEAGE
                     VEVEAEVTEATRKGQVLIPHGFGLVYQGRAYGANVNRLTKTTNRDRFAATPIHRYVPC
                     RIEPA"
     gene            2291..3535
                     /locus_tag="PLT69_02765"
     CDS             2291..3535
                     /locus_tag="PLT69_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HOJ13397.1"
                     /translation="MNTQPGTAPDPKARVTQPLQAAPVDRPHVSGGAAGARIRFNRNE
                     LAGAFGDMGTDIPLLIGVTLACGLDATSVLVMFGLMQVFTGLSYRMPMPVQPLKAMAA
                     IVISQQVTAAQLHGAGLAIGLVMLLLALTGMLDWLGRVVPKCVIRGIQLGLGLQLAGV
                     ALGKFVLSDGAMGYVLALAAFVATVALLGNRRFPAALAVVVMGLVYAFAFNLEGDALA
                     SSLGFHLPSLASFSPADMATGFVVLALPQIPLSLCNSVLATRQLAVDLFPERPLSIKR
                     IGLTYAVMNLVNPWFGGVPTCHGSGGMAGHFAFGGRTGGSVVIYGALLVTCGLFLGTG
                     FDHVVKAFPLPMLGVLLLFEALALIWLIRDVVGEANGFPVTVLVGLVAAGLPYGYVIG
                     LIAGTVLWRLGRRARLVSPDAP"
     gene            4104..4679
                     /locus_tag="PLT69_02770"
     CDS             4104..4679
                     /locus_tag="PLT69_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006005310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOJ13398.1"
                     /translation="MKIGQYAFDEFVDKVREFHGSAAPGVIAGGVMVDIAREHLPQGK
                     LYDVVCESAKCLPDAVQLLTPCTLGNGWLKVCETSRYALAFFDKYTGEGVRVFLDASA
                     LGPWPALRAWFLKEKAKAEQDQAEIVEEIKKAGRSIYGIEHVRVKRDFLASRTRRHRP
                     VSLCPSCGEAYMADLGQRCPACMGTGPYETG"
     gene            5090..5491
                     /locus_tag="PLT69_02775"
     CDS             5090..5491
                     /locus_tag="PLT69_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13399.1"
                     /translation="MARAFMARTTLIAALLCAAIPLSGLAMTPMNDEDLDLVSGQSGV
                     SIFVDVTISIHIDAIAWGDEDGIGGAQRTRSAGDRPFNDRSTSFSISVCPNLPSPLLM
                     EVMKRYSSSHGVCFFPDTGAISIENGVFQRR"
     gene            complement(6034..>6613)
                     /locus_tag="PLT69_02780"
     CDS             complement(6034..>6613)
                     /locus_tag="PLT69_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13400.1"
                     /translation="KNYSHLQFDVCAVHVKIDEYDTAPLREYCEKLSVEYIETPLKVD
                     LRAYPRSPCHVCSSFKRGAIGEALEGRGVRKVAFGHHADDAAETFLMNIAYNRRLASL
                     TPKVMVPEGSFEIIRPMVYLPGPTVKRLHNHLRLPVLDYLCPHGEKSAREVMRTAIAR
                     IEETAELREFSRMVVDALENVDYSSLWPSLRV"
BASE COUNT         1114 a         2227 c         2242 g         1030 t
ORIGIN      
        1 cgagcgcatc ctggccgggg ctgacgcgca cgcgcgcaag agaggaggga aaaggcatgg
       61 gtgaatggaa gaagacgggg tgcgtgctct gcgcgcagaa ctgcgggctc gaggtgtacg
      121 tggagaacaa ccgcatcgtc aaggcgaggg gcgacaggga caacccccgc tccaagggct
      181 acctgtgccg caagggcgcc aacatcgcct tccaccagca ccacgcggac aggatctcct
      241 ctcccctcaa gagggtgggc gaccgcttcg agcgcatctc ctgggaccag gcgatcgggg
      301 agatcgccga gaggctcaag gcgatcgtcg agacccacgg cccgaggagc ctcgcataca
      361 tgggcggagg gggacagggc tgccacttcg aggccgcatt cggtgtgagg ctcatgcggg
      421 cgctcggctc ccgttaccac tacaacgccc tcgcccagga gcttaccggc atcttctggg
      481 cctcgggcag gctcgcgggc aggcagtacc tctacgccat cccccacgag gaggaagcgg
      541 acatgctcgt ggccgtgggg tggaacggca tgcagagcca ccagatgccg cgcgcgcccc
      601 tggtcttgag ggagttctcc aagaacccgg acaagctgct cgtggtcatc gacccgcgca
      661 ggtccgagac ggccgagatc gccgacatcc accttcccgt gaggcccggg acggacgcgc
      721 tcctcgccag ggccatgatc tcgatcatcc tcgaggaggg ctggcaggac agggagtacc
      781 tcgagctgca tgtccagggc ttcgaggaga tcgagccctg gttcaggggg ttcgacgccc
      841 gcgacgcggt gagggtctgc gagctcgact atgacgccgt gcgtgggctc tgcagactca
      901 tgaccacgcg caggtggtgc atgcacacgg acctcggcgt gtacatgaac cgccacagca
      961 cggcgaccac ctacctcctg ctcctcctgc tcgccgtctg cggcaggctc tgcgtgccgg
     1021 gcggcaacgt gatccccggc cacctcatgc ccatcggcgc gcacaccgac gagcgcgacc
     1081 cgaagaactg gaagaccgtg accacggggt tccctgcgct catgggcgtg taccccccca
     1141 acgtcatgcc cgaggagatc ctctcggacc acccggagcg gctcagggcg gtgctgtgct
     1201 gccagtcgaa cccgctgagg tcgttcgcgg acaccaccat gtacgagaag gcgttcggaa
     1261 ggctcgacct gctcgtcacc tgcgagctct ccatgaccga gacggcgcgc ctgtcccact
     1321 acgtcctgcc cgcgcgctcg ggctacgagt cgtgggacgg gaccttcttc gcctggacct
     1381 tccccgagat ctacttccag atgaggcggc ccgtggtcga gcccgagggc gagaggctgg
     1441 agctgagcag catccacacg atgctcgccg agaaactcgg catcgtccct cccatacccg
     1501 actctctcgt cgaggccgcc tccaaggacc gtctcacctt cggcgcggag ctcatgaact
     1561 acgcgaccgc cgagccgagg gcgcttgcga acatgccgtt catcctcgcg aagaccctgg
     1621 gagagcggct cggctcaccc aacctggccg ccctgtgggg actcctccag acggcgccca
     1681 agtccttcag gaaggcggct gcgagggccg ggttcacgcc cggcatcacg atgggggagg
     1741 agatcttcca ggcgatcctc gaccacccgg aggggttgat cctggggctc gccgacaagg
     1801 aggacaactt ctcgatgatc gcccacgagg acaagaaact ccacgtctcc atcccggaga
     1861 tggacccctg ggtgagggag atcgagcccg gccgcgaaga agaggcgctg gcgccccacc
     1921 cagactaccc cctcatcctc caggccggca gacacatgag cgcaaacgcg aacaccctca
     1981 tgcgcgaccc tgcatggaac gagggcagac gtgcctgcac cgtgagcgtc cacccggagg
     2041 acgccggcag gatcggcatc tcggacggcc agatcgtgcg cgtgatcacc gaggccggcg
     2101 aggtcgaggt ggaggccgag gtgaccgagg cgacgaggaa gggacaggtc ctcatacccc
     2161 acgggttcgg gctcgtctac cagggcaggg cctacggagc caacgtgaac cggctcacga
     2221 agaccacgaa ccgcgacagg ttcgcagcca cgcccatcca caggtacgtg ccctgcagga
     2281 tagagcccgc atgaacacgc aaccgggcac cgcgcccgac cccaaggccc gggtgacgca
     2341 gcccctgcag gccgcaccgg tggaccgccc ccacgtgagc ggtggtgccg ccggcgccag
     2401 gatacggttc aacaggaacg agctcgcggg cgcgttcggg gacatgggca cggacatccc
     2461 cctgctcatc ggggtgacgc ttgcctgcgg gctggacgcg acgagcgtgc tcgtcatgtt
     2521 cggcctgatg caggtcttca cggggctttc ctacaggatg cccatgccgg ttcaacccct
     2581 gaaggccatg gcagccatag tgatatccca gcaggtcacg gccgcccagc tccacggcgc
     2641 ggggcttgcc atcggcctcg tcatgctcct gctcgcgctc accgggatgc tcgactggtt
     2701 ggggagggtg gtacccaagt gcgtgatcag gggtatccag ctcgggctcg ggctccagct
     2761 ggcaggcgtg gcgctcggca agttcgtgct gtcggacggt gccatgggtt atgtgttggc
     2821 gttggccgcc ttcgtcgcca ccgtggcgct cctcgggaac aggcgcttcc cggccgcgct
     2881 cgcggtggtc gtcatggggc tcgtctacgc cttcgccttc aatctcgagg gcgacgccct
     2941 ggcctcgagc ctcggcttcc acctgccatc gctcgcctcg ttcagccccg cggacatggc
     3001 gaccggcttc gtggtgctcg ccctgcccca gatccccctg tcgctgtgca actcggtgct
     3061 cgccacgcgc cagctcgccg tcgacctctt cccggagcgc cccctgtcga tcaagaggat
     3121 cgggctcacc tacgccgtca tgaacctcgt caacccgtgg ttcggcggcg tgcccacctg
     3181 ccacggctcc ggcggcatgg ccggccactt cgccttcggc gggcgcaccg gcgggtcggt
     3241 ggtcatctac ggcgccctgc tcgtcacgtg cgggctcttt ctggggaccg gcttcgacca
     3301 cgtcgtgaag gcgttccccc tgcccatgct cggcgtgctg ctgctcttcg aggccctcgc
     3361 cctcatctgg ctcatccggg acgtcgtggg cgaggcgaac ggcttccccg tcaccgttct
     3421 cgtgggcctg gtggcagccg ggctccccta cgggtacgtg atagggctga tcgcgggaac
     3481 cgtgctctgg cgcctcggca gacgggcgag gctcgtgagc cctgatgcac cgtgagccgc
     3541 aaggggcggg ccggcgagag gaccgtgcgt gcattcacga acacgaggac tttggatccg
     3601 gcacggcggc gcgcacggcg ttttcccggg catgcgcccg agcggggatc aggcccgtca
     3661 gggtcctcct tgacgcgccg gggccgggcg gctacgatcg ggggtgacgg gaaagagaca
     3721 ggctgtgtgt accgcttgcg ggacgacgac gcaccaggag gccgaggcgg gcatgcccga
     3781 cgggaccggg cggttctctc catgagcgca cggcgccgct ttcgtggcag agccgggaga
     3841 cgacccggta gacatccgga tcggccggtt tccccctgcc aggaagaaag cgggcgagag
     3901 aaaagggaag aacctggggg cgccttcgta caggggcgca cgtccccggc gcccgagggc
     3961 acgtcaaggg acccctcgtg tccaggtgct gttcgcgatc ccagatgtcg atgcggcgcc
     4021 tgtaccgaaa gacgggcacc gcgcatgcgg ccctcgctga accacaggcg ggccctcggg
     4081 acgttcgaga cagggaggcg gcggtgaaga tcggacagta cgcattcgac gagttcgtgg
     4141 acaaggtgag ggagtttcac ggcagcgccg cgccgggcgt catagcgggc ggcgtcatgg
     4201 tggacatcgc cagggaacac cttccccagg gcaagctcta cgacgtcgtc tgcgagagcg
     4261 cgaagtgcct gccggacgcg gtccagctcc tgacgccgtg caccctgggg aacggctggc
     4321 tcaaggtatg cgagacctcg cgctacgcgc tcgcgttctt cgacaagtac acgggagagg
     4381 gcgtccgcgt gttccttgac gcctccgccc tcggtccgtg gcctgccctt cgcgcctggt
     4441 tcctcaagga gaaagcgaag gccgagcagg accaggccga gatcgtcgag gagatcaaga
     4501 aggcggggag gagcatctac ggcatcgagc acgtgcgcgt gaagcgggac ttcctcgcat
     4561 ccaggaccag aaggcatagg ccggtgtccc tctgcccttc gtgcggggag gcctacatgg
     4621 ccgacctcgg ccagcgttgc cccgcatgca tgggcacagg cccctacgag acggggtgag
     4681 gggctctgca tgcggcggtc tcccgggtga ccggcaggcc gcattcgaca ggcgaccccg
     4741 ttccccgaag acctcgaggc catgcggcca tgcccgtgac aagggccgtg tcgccgcggc
     4801 agaccccccc aggggtctgt gcgggcggtg caaccggaat ggctcacggg ccgtaccggg
     4861 gtccctgagg ccgcctgggg gccggcgcag tgcgatgcgc gaccggcagg atgccgcctt
     4921 cgaccggacg ccctacgggg ccgacggtcg tggtctcacc ccatggtttg tcgcggccgg
     4981 gtttccgtat ggaacgggtg gactttcggt gaggaatact gtagtatcgg ggaaacccac
     5041 aagccgtccc gtgtgtctcg ggcggctcgg ggctcgggag gcaagcggca tggcaagggc
     5101 gttcatggcg agaacgacgc tcatcgcggc cctcttgtgc gccgccatcc ccctttccgg
     5161 cctcgccatg acacccatga acgacgagga cctcgatctc gtctccggcc agagcggcgt
     5221 gagcatcttc gtggatgtca cgatcagcat tcacatcgac gcgatcgcct ggggcgacga
     5281 agacggcata ggcggcgcgc agaggacgag gtcggcggga gaccgcccgt tcaacgatcg
     5341 ctccacctcc ttcagcatca gtgtctgccc gaaccttccg agcccgctgc tcatggaggt
     5401 catgaagcga tacagctcgt cccacggcgt ctgcttcttc cccgacacgg gggcgatctc
     5461 catcgagaac ggcgtcttcc agagacgttg aaggcgtgag cctccgtggg cgatcgcgcc
     5521 tgtctcggcg acatcgatct ccttctgtct tccccagacg ttgaaggcgc gcgcgtccgt
     5581 ctgtgcgcct cggcatgccc ctgggcatcc cagacgccac aggccggtga cacccccagg
     5641 gccctctccc gtgagacgtg cggccggcac tatccgaccg ccgcccgatc ggacccaagg
     5701 agcccaccac aagagggtgc acgaccgacc gaaccgaagg ccgcctggtg cctggcaccc
     5761 tggggagcaa aggacacctg tgcccaggtc gtggaaagga acccggggca cgcgcgagaa
     5821 aagaccgggc ggcctgacgc ctgcgctttc ggtgtcgcac aaagcggtga caacccgagt
     5881 tcgtgtgtgt actggcatct tcgtgaccgg ggcctcccgc ccggccgtgg gccccaggga
     5941 gaaactcggg ccaacgtacg agaaacgagc acccccgcag tcgggacacg gaggccggcg
     6001 agagacccgg ggttcgcgtg cgagcaacac gggtcacacc ctgagcgagg gccagagcga
     6061 cgagtagtcc acgttctcga gtgcgtccac gaccatgcgg gaaaactccc ggagctcggc
     6121 cgtctcctcg atacgcgcga tggcggttcg catgacctct ctcgcggatt tctcgccgtg
     6181 ggggcagagg tagtccagga cgggcagcct caggtggttg tgcagcctct tgaccgtggg
     6241 tccgggaagg tacaccatgg gccggatgat ctcgaacgag ccctcaggga ccatcacctt
     6301 gggggtgagg ctcgcgagcc tccggttgta ggcgatgttc atgaggaacg tctccgcggc
     6361 gtcgtcggcg tggtgcccga acgccacctt cctcaccccc ctgccctcca gggcctcccc
     6421 gatggcgccc ctcttgaagg atgaacatac gtggcagggc gatcgggggt acgccctgag
     6481 atcgaccttg agcggggtct cgatgtactc caccgacagc ttctcgcagt actccctcag
     6541 gggtgcggtg tcgtactcgt cgatcttgac gtgaacggcg cacacgtcga actggaggtg
     6601 ggagtagttt ttc
//
LOCUS       DAOSCR010000064         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7563_length_6518_cov_3.269998, whole genome shotgun sequence.
ACCESSION   DAOSCR010000064 DAOSCR010000000
VERSION     DAOSCR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7563_length_6518_cov_3.269998"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLT69_02785"
     CDS             <1..434
                     /locus_tag="PLT69_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ13401.1"
                     /translation="HVRFAGEVASPLIPYGCCDILVSFEELEAARYPPYMNGGTTVIV
                     NRFRLAPPSVIAGKEPYPDVVPILKGCSGNVYLADGTAVAQALGERRALNLVLMGVLS
                     RFIEIPETLWAEVIADMVPERIREVNLEAFKRGRTIDLVRT"
     gene            457..1758
                     /locus_tag="PLT69_02790"
     CDS             457..1758
                     /locus_tag="PLT69_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ13402.1"
                     /translation="MIFNEEFETLPREALASLQLKRLKQVVERVYHTVGFYRKKFDEA
                     GVKPEDIRSLEDLGRLPFTTKQDLRDNYPFGLFSVPMSNIVRLHASSGTTGKPTVVGY
                     TRRDIETWAELMARSLAAAGLTKNDIVHNAYGYGLFTGGLGVHYGAERLGASVIPMSG
                     GNTKKQILILQDFGPTAICCTPSYTLYLAEVGEEMGVDMRSLRLRVGILGAEPWGERM
                     RKEIESRLDITALDIYGLSEIMGPGVAMECIEGKNGLHIFEDNFIVEVIDPSTGEVLP
                     DGQAGELVFTTLTKEAFPLVRYRTKDLSRLMREVCRCGRTFVRMERVMGRSDDMFIIR
                     GVNVFPSQIESVLMEIEGLEPHYMLIIDREGALDTLEIQVEVSERIFSDEIRRLQNLE
                     RRIAKDIKDYIGITAKVKLVEPKTLQRTEGKAQRVVDKRPR"
     gene            1770..2201
                     /locus_tag="PLT69_02795"
     CDS             1770..2201
                     /locus_tag="PLT69_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ13403.1"
                     /translation="MKVEQISIFLENKPGGLSGVTRVLKDAGINIRALSLADTSDFGI
                     LRLIVDDVNKAKKVLNDNGFAVGRTHVIAVEVPDRPGGLHSILDLLTRHNINVEYMYA
                     FVERSGENAVIIFRFDDIDQAIQVLLDGGFTVLRGEQVYSL"
     gene            2246..2650
                     /locus_tag="PLT69_02800"
     CDS             2246..2650
                     /locus_tag="PLT69_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13404.1"
                     /translation="MARIVRHEVRDGFGRENALGMIGRVYLVLGIAGALFSVVLSAAL
                     GQLFWIAAGVLFLAFGVAFHVLFGAFGEVIALLKRSWGVKTDFKVSGEASGTIFLCSE
                     CGAMVYESSERCPRCSEEFVPGGEGAARDTGS"
     gene            2685..3734
                     /locus_tag="PLT69_02805"
     CDS             2685..3734
                     /locus_tag="PLT69_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOJ13405.1"
                     /translation="MKRLVIVAVCMFVVVALSASCSKKEEQAQQPAQEVKAEPVKLTY
                     SIFFPPTHPQCKAAQDWASEIEKRSGGAVKIDVFPGGTLTSANDCYDGVVKGISDIGM
                     SCFAYSRGRFPVMEAVDLPLGYPSGTAATKTANDYYNEMKPAELEDVHVLYLHAHGPG
                     LLHTKKPVKTLKDIKGMKIRSTGLSAKVVEALGGVPVAMPQPGTYEALQKGVVDGTFA
                     PMETMKGWKQGEVVKTTTDCRTIGYTTAMFVVMNKDKWNQLPPEVQKVFTDVSNEWIA
                     VHGKAWDDADVEAKAYVEGLGNTYIVLDPAEAKKWEKAVENVLKSYVEETKAKNIDGE
                     KAVSLLRELIKKNAQ"
     gene            3804..4298
                     /locus_tag="PLT69_02810"
     CDS             3804..4298
                     /locus_tag="PLT69_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOJ13406.1"
                     /translation="MISFIRIEKFTARLSERVNWAGAAALVFMMLITVADVAGRLFKS
                     PIPGTYELVGFTGGAVIAMALPYTSVMKGHIAVEILVQRLPWRARVAVNALNALVSML
                     LFAVIAWQCVRLAQSMEASSEVSLTLQLPVYPFVYGIAAGCALLSVVLGVEFIRQLRG
                     AEAE"
     gene            4295..5596
                     /locus_tag="PLT69_02815"
     CDS             4295..5596
                     /locus_tag="PLT69_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOJ13407.1"
                     /translation="MTPTQTGIVGLVALVVLLFSRMPVAFIMALVGFWGFASIVSTDA
                     ALNLLAKDVFSTFTSYSLTVIPLFVLMGQVAFHSGISRRLFDTAHKFMGHLPGGLAIA
                     TIGACAFFASICGSTNATAATMGSATLPEMKRYDYHPELATGVVASAGSLGILIPPSV
                     IFIVYGILTEQSIGKLFISGILPGVILALLFVLTVLVWVKLRPELGPRGPRFPLRERM
                     ASLAGVSETLVIFVLVMGGLFAGVFTPTEAGAMGAFFTIVVAVARGQLSWKGFLQALS
                     ETTRITCMIMVIVAGATVFGHFLAVSRIPYDIAGWISGFDLPAWVIMGLIILGYVVGG
                     CFMDALALIMLTIPIFYPVVLKLGFDPIWFGVIIVLVTQIGVVTPPVGVNAYVVSGVA
                     RDVPLQVIFKGCIPFIAAMVILTFLLIPFPQIATFLPGLIR"
     gene            complement(5827..6282)
                     /locus_tag="PLT69_02820"
     CDS             complement(5827..6282)
                     /locus_tag="PLT69_02820"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ13408.1"
                     /translation="MTAYQLTVPVENRPGRLAQVSGVLAREKINIRAITISSFGDKGF
                     FNILVDDPKLALKALKKEGIEAELKEIIAVLIDDKPGGMDSLAQILARQGINIENAYG
                     FVLESNKTAVFVIDVSDLEGTQRILRDHGFKTLSPEALAAIEPFHYMRY"
BASE COUNT         1156 a         2023 c         2154 g         1185 t
ORIGIN      
        1 cacacgtgcg cttcgccggg gaagtggcct ccccgctcat cccctacgga tgctgcgaca
       61 tcctcgtctc gttcgaggaa ctcgaggccg ccaggtaccc cccttacatg aacggaggta
      121 ccaccgtgat cgtcaaccgg ttcaggctgg ctcccccttc ggtcatagca gggaaggaac
      181 cctacccgga cgtggtgccg atcctgaagg ggtgttcggg aaacgtctac ctggccgacg
      241 gaacggccgt ggcccaggcc ctcggcgagc gccgggcgct caacctggtg ctcatgggag
      301 ttctctcgag gttcatcgag ataccggaga ccctctgggc ggaggtgata gcggacatgg
      361 tgccggagcg catccgcgag gtgaatctcg aggcgttcaa gaggggcagg acgatcgacc
      421 tcgtccgcac atgaacagga caggaggttc ttcgcgatga tcttcaacga ggagttcgag
      481 accctgccga gggaggccct ggcatccctc cagctgaaga ggctcaagca ggtcgtcgag
      541 agggtgtacc acaccgtggg gttctaccgg aagaagttcg acgaggcggg ggtgaagccc
      601 gaagacatca ggtccctcga agacctcggc cgcctgccct tcaccaccaa gcaggacctc
      661 agggacaact acccgttcgg cctcttcagc gtgcccatga gcaacatcgt ccgcctccac
      721 gcttcgtccg ggacaacggg caagcccacg gtggtgggct acaccaggag ggacatcgag
      781 acctgggccg agctcatggc caggtctctg gcggccgccg gcctcacgaa gaacgacatc
      841 gtccacaacg cctacgggta cggcctgttc acgggcggcc tcggggtgca ttacggcgcc
      901 gagcgtctcg gcgcgagcgt gatccccatg tccggcggga acaccaagaa gcagatcctg
      961 atcctgcagg attttgggcc gaccgccatc tgctgcacgc cctcctacac cctctacctg
     1021 gcggaggtgg gagaggagat gggtgtcgac atgcgctctc tcaggctcag ggtggggatc
     1081 ctcggggccg agccctgggg agagcgcatg aggaaggaga tcgagtcccg gctcgacatc
     1141 accgcgctgg acatctacgg gctgtccgag atcatgggac cgggcgtggc catggagtgc
     1201 atcgagggga aaaacgggct gcacatcttc gaggacaact tcatcgtgga ggtcatcgac
     1261 ccgtccaccg gagaggtgtt gccggacggc caggcaggcg agctcgtctt caccacgctc
     1321 accaaggagg ccttccctct cgtccgctac cgcaccaagg acctctcgag gctcatgagg
     1381 gaggtgtgcc gctgcgggag gaccttcgtg cgcatggaac gcgtcatggg caggagcgac
     1441 gacatgttca tcattcgggg cgtgaacgtc ttcccgtccc agatcgagag cgtcctcatg
     1501 gagatcgagg gcctcgaacc ccactatatg ctcatcatag accgggaagg agcgctggac
     1561 accctcgaga tccaggtgga ggtgagcgag cggatcttct cggacgagat caggaggctc
     1621 cagaacctcg agcggcgcat agcgaaggac atcaaggact acatcggcat caccgcgaag
     1681 gtgaagctcg tggaacccaa gaccctgcag cgcaccgagg gtaaggcgca gcgggtggtg
     1741 gacaagagac ccagataagg gggtgtccca tgaaggtcga acagatttcc atcttcctcg
     1801 agaacaagcc cggcggcctg tcaggcgtga caagggtcct caaggacgcg ggcatcaaca
     1861 tcagggccct gtcgctcgcc gacacgagcg atttcgggat cctgaggttg atcgtggacg
     1921 acgtgaacaa ggcgaagaag gtcctgaacg acaacgggtt cgcggtggga cgcacccacg
     1981 tgatcgcggt cgaggtgccg gacaggcccg gcgggctcca cagcatcctc gatctcctca
     2041 cgcgccacaa catcaacgtg gagtacatgt acgccttcgt ggaaaggagc ggcgagaacg
     2101 cggtgatcat cttccgcttc gacgacatag accaggcgat ccaggtgctc cttgacggcg
     2161 ggttcacggt gctcagggga gaacaggtct acagcctgta ggcccggctt ccccagccgt
     2221 gatcaaggcg cacacgggag aaggcatggc ccgcatagtc aggcacgagg tgagagacgg
     2281 tttcgggagg gagaacgccc tcggcatgat cggcagggtc tatctcgtgc tcgggatcgc
     2341 cggggcgttg ttctccgtcg tcctgtcggc ggcgctcggc cagctctttt ggatcgcggc
     2401 aggggtcctc ttcctcgcgt tcggcgtcgc cttccacgtg ctcttcgggg ccttcgggga
     2461 ggtcatcgcg ctgctcaaga ggtcgtgggg ggtaaagacg gatttcaagg tctccggcga
     2521 ggcctcgggc acgatcttct tgtgctccga gtgcggggcc atggtgtacg agtcgagcga
     2581 acgatgtccg aggtgttccg aggagttcgt tcccggcggt gagggggcag caagggatac
     2641 gggatcctag aagcatattc attaccatga aggggggagg ttcgatgaag agactggtta
     2701 tcgttgcagt gtgcatgttc gtcgtcgtgg cgctgtcggc gtcctgctcg aagaaggagg
     2761 agcaggcgca gcagccggca caggaggtca aggccgagcc cgtcaagctc acctacagca
     2821 tcttcttccc gcccacccac ccgcagtgca aggcggccca ggactgggcg agcgagatcg
     2881 agaagcgcag cggcggggcg gtcaagatag acgtcttccc gggcggtacg ctcacgagcg
     2941 ccaacgactg ctacgacggg gtggtcaagg ggatctccga catcgggatg tcctgcttcg
     3001 cctactcgag ggggcggttc ccggtcatgg aggcggtcga cctcccgctc ggctacccgt
     3061 cggggacggc cgccacgaag acggcgaacg actactacaa cgagatgaag cccgccgagc
     3121 tcgaggacgt gcacgtactc tacctgcacg ctcacgggcc ggggctgctc cacaccaaga
     3181 agccggtcaa gaccttgaag gacatcaagg gcatgaagat ccgttccacg ggcctgtctg
     3241 cgaaggtggt cgaggccctc ggcggcgtgc cggtggccat gccccagccc gggacgtacg
     3301 aggcgctcca gaaaggggtc gttgacggca cgttcgcgcc catggagacc atgaagggct
     3361 ggaagcaggg cgaggtggtc aagaccacca ccgactgcag gacgatcggg tacaccaccg
     3421 ccatgttcgt ggtcatgaac aaggacaagt ggaaccagct cccgcccgag gtccagaagg
     3481 tcttcaccga tgtcagcaac gagtggatag ccgtccacgg aaaggcatgg gacgacgccg
     3541 acgtcgaggc caaggcctac gtcgaggggc tcggcaacac ctacatcgtg ctggatcccg
     3601 cagaggccaa gaagtgggag aaggcggtcg agaacgtgtt gaagtcctat gtcgaggaga
     3661 ccaaggccaa gaacatagac ggcgagaagg cggtctcgct ccttcgggag ctcatcaaga
     3721 agaacgcgca gtaggaagag cggcgcatgc gcggcccctc ccgatggagg cggccgcgca
     3781 tgcagcccgg ggtggtaccc tgcatgattt cgttcatcag gatagagaaa ttcacagccc
     3841 gcctctccga gagggtcaac tgggcggggg ccgccgccct cgtgttcatg atgctgatca
     3901 cggtggcgga cgtcgcgggc aggctgttca agtcccccat cccggggacc tacgagctcg
     3961 tgggcttcac cggcggggcg gtcatcgcca tggccttgcc gtacacgagc gtgatgaagg
     4021 ggcacatcgc ggtggagatc ctcgtccaga ggctgccctg gcgggcgagg gtggctgtca
     4081 acgccctgaa cgcactggtg tccatgctcc tcttcgccgt gatcgcctgg cagtgcgtga
     4141 ggctcgcaca gagcatggag gcgagcagcg aggtgtcgct gacgctccag ctgccggtct
     4201 acccgttcgt ctacggcatc gccgcggggt gcgccctctt gagcgtggtg ctcggtgtgg
     4261 agttcataag gcagctcagg ggagcggagg ccgaatgaca cccacccaaa cagggatcgt
     4321 cgggctcgtt gcgctcgtcg tgctgctctt ctcccgcatg cccgtcgcgt tcatcatggc
     4381 gctcgtcggg ttctgggggt tcgcgtcgat cgtgtcgacg gacgccgcgt tgaacctcct
     4441 cgccaaggac gtcttctcga cgtttacctc ctacagcctc accgtgatac cgctcttcgt
     4501 gctcatgggc caggtcgcgt tccattcagg catcagcagg cgcctcttcg acacggccca
     4561 caagttcatg ggccacctgc cgggcggcct ggccatcgcg accatcgggg catgcgcctt
     4621 cttcgcgtcc atatgcggct ccaccaacgc caccgcggcc accatgggct cggccacgct
     4681 gcccgagatg aaacgctacg attaccaccc cgagctggcc acgggcgtgg tggcctctgc
     4741 cggcagcctc ggcatcctca tcccgccgag cgtgatcttc atcgtgtacg gcatcctgac
     4801 cgaacagtcc atcggcaagc tcttcatatc ggggatcctc ccgggggtga tcctggccct
     4861 cctgttcgtc ttgacggtcc ttgtctgggt gaagctcagg cccgaactgg gtcccagggg
     4921 gccgaggttt cccctgaggg agaggatggc gtctttggcg ggcgtgtcgg agacgctcgt
     4981 catcttcgtc ctcgtcatgg gcgggctctt cgccggggtc ttcaccccca cggaagcggg
     5041 ggccatgggc gccttcttca ccatcgtcgt cgccgtggcc aggggccagc tttcctggaa
     5101 gggcttcctc caggccctgt ccgagaccac ccgcatcacg tgcatgatca tggtgatcgt
     5161 cgccggggcc accgtgttcg ggcacttcct cgccgtgtca cggatcccct acgacatcgc
     5221 gggctggatc tccgggttcg acctgccggc ctgggtcatc atgggcctga tcatcctcgg
     5281 gtacgtggtg ggcgggtgct tcatggacgc cctggcgctc atcatgctca ccatacccat
     5341 cttctacccc gtggtgctga agctcggctt cgaccccata tggttcgggg tgatcatcgt
     5401 cctcgtcacg cagataggcg tggtgacccc gcccgtgggg gtgaacgcct acgtggtcag
     5461 cggcgtggcc cgcgacgtcc cgctgcaggt gatcttcaag ggctgcatcc cctttatcgc
     5521 ggccatggtc atcctgacct tcctgctcat acccttcccc cagatcgcca cgttcctgcc
     5581 gggtctcatc aggtagatac gcacccaggc ccggccgcag ccgggggtgt ccacgcgatc
     5641 gtcgcatatg cccgatggag ggctggggca tgaccgttgc aggcatgggg caagcgcttt
     5701 gagcggatgc tcggaaccgg gcttcgatcc ccaagcgcct ttgagtcggt gagggacccg
     5761 cccgtttccc tccaggccga tggttccaaa acgttcgaga gggtggggcg cctaccgaag
     5821 gcgtcttcag tacctcatgt agtggaacgg ctctatggcg gcaagggctt ccggcgagag
     5881 cgtcttgaac ccgtggtccc tcaggatccg ctgggtgcct tcgaggtcgg atacgtcgat
     5941 gacgaagacc gccgtcttgt tgctctcgag cacgaacccg taggcgttct ctatgttgat
     6001 cccctgcctg gccaggatct gggcgaggct gtccatgccg cccggtttgt cgtcgatgag
     6061 gaccgcgatg atctccttga gctccgcctc gatgccctct ttcttcaagg ccttcagggc
     6121 gagcttcggg tcgtcgacga ggatgttgaa aaatcccttg tcgccgaacg acgagatggt
     6181 gatggcgcgg atgttgatct tctccctggc aaggacgccg gagacctggg caagcctgcc
     6241 cggcctgttc tcgactggca ccgtgagctg gtatgcggtc atggttcacc ccgtggcgtg
     6301 tgtgtcgtct tcctgtcctg tcgtctcagg aatgtagtgt acgtcccgga cgcgacatgt
     6361 caaggccaat gtccgggcct cgggaaggga cggccgggtg gggctccctg tccggtcccg
     6421 ggggcgttcc ccctttcagg tgttcccggc actgcacgcc cgtcccgtgc atcctggacg
     6481 aggcggcctt cccgctcggc acgttgcggt cccggtcg
//
LOCUS       DAOSCR010000065         6504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7578_length_6504_cov_3.419135, whole genome shotgun sequence.
ACCESSION   DAOSCR010000065 DAOSCR010000000
VERSION     DAOSCR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7578_length_6504_cov_3.419135"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLT69_02825"
     CDS             complement(<1..212)
                     /locus_tag="PLT69_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOJ13409.1"
                     /translation="MGVKVSERICLELQKAIFRGEYPPNSRLPSERELARRFEASRAA
                     VREAVVHLSEIGLVRTLPQSGTYVTD"
     gene            683..868
                     /locus_tag="PLT69_02830"
     CDS             683..868
                     /locus_tag="PLT69_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13410.1"
                     /translation="MADHPCCPSCGRRAKKALTSNHFPVYRCRSCGRVFCMECSNGSC
                     PSCGSNSRDKAGNVYAR"
     gene            960..1364
                     /locus_tag="PLT69_02835"
     CDS             960..1364
                     /locus_tag="PLT69_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13411.1"
                     /translation="MTKSELAARYTDFLTDQGFRPEIDKDGDVKFLFEGGLYYILVSE
                     KDPEFFRLVYPNFWEIESDEEKMKATIAASFATADTKVAKVILVGNNVWASVESFFES
                     PDHFKGVFRRSISAIRTAVSSFRDNMKQLSGV"
     gene            1718..2170
                     /locus_tag="PLT69_02840"
     CDS             1718..2170
                     /locus_tag="PLT69_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13412.1"
                     /translation="MARSWYTLYRSVALSFLLAVCACSSSGGGDELPGSGEHVVTYKV
                     EGGSGIITSASISYTDENGEEQHLQHVSLRDANWSYSFRADDFAPLSVSAMLEGEGTS
                     TTLYVTIEVDDACWVENRTFVAGALLTVSSTAHEILTTCPNHGGTTVQ"
     gene            2183..2836
                     /locus_tag="PLT69_02845"
     CDS             2183..2836
                     /locus_tag="PLT69_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13413.1"
                     /translation="MPAAQASPKGQRTAGRKAGRKVSTARKDEIIDAAERVFASKPFP
                     RVSMRDIAREAGISPALIYRHFADQQHLFLEAFFRGAERLMGRIDELFKVHPESGLED
                     ASAAFISFLAENEHYFKMMTHFMLEGTVHEGLLERLNSIERSVLDRFDELFRKAGAVG
                     DVRILSHCFFASLNGILITFRRHPGRTKEEVVSHMHRLGITLARLFAHALRTANVGP"
     gene            complement(2955..4547)
                     /locus_tag="PLT69_02850"
     CDS             complement(2955..4547)
                     /locus_tag="PLT69_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13414.1"
                     /translation="MSLPDRNNPYTFDAFLEWRRRFNYYLDDEFFQRVLRRYAGDDFP
                     AIHEELKAFSGKVSYRWRDLAEAAAVPEKRPYVVHYDGHNHRIDRIVRPMEVLVMEKE
                     VFSEALFSKRTNPWLKLAKMYLIYQNGEACIACPLTCTEGLVALLERFADTPETRRIL
                     MHCSEGIDGDFGIGAQYLSEIQGGSDVPANLLEAVEENGTWRLYGKKFFCSATHADYA
                     VVTAKPAGSEKVALFVVPSWLEGDKEKEIRNGCTIDRIKLKMGTSELTTAEITYNGAK
                     AYPVGPLDRGVANVVGIVLTYSRLTVGLSGAAGMTRGAREAKAYASFRDAFGVRLEHF
                     PMLANQLEWMDTSAKRTTAGAFMLYREFLGLEGGLKGGLLADEDLSMRKRRFCVRELI
                     MLQKITASWDATDVARMAMSVFGGHGVMEDFSSLPRMFRDAAINELWEGPRNVLLTQM
                     HRDFQRAAPWYAPGAFVRDILKGADTGLVEELAAEMELLTSHPSLFTMDDKTKDVCRR
                     WDSLCHRLFHAYQDLALAEVLS"
     gene            complement(4908..5348)
                     /locus_tag="PLT69_02855"
     CDS             complement(4908..5348)
                     /locus_tag="PLT69_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOJ13415.1"
                     /translation="MGVVEYGRMKTIGEALARADKNMARVMKIRSRWAEIAGEVLASH
                     TEPVLIKSSKLHVLCDSPAWAQQVGLLAGNIVERVKGVVGLKLSGVEASFGKPGRPRP
                     QRPPEPRTAPFKVDIDPSDIEKVRDPKLADLLRQLARAGRENDA"
     gene            complement(5544..6491)
                     /locus_tag="PLT69_02860"
     CDS             complement(5544..6491)
                     /locus_tag="PLT69_02860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ13416.1"
                     /translation="MLVSGEGEGIRLDAFVAGAIGVGRGPAARAVEQGRIRVDGLARK
                     PSFRLKQGMRVQGACPAPRQAGPVEPVGIPLEVLYEDEWIVVVNKPAGMSVHPGAGAS
                     GPTLAGALIARYPEMALVGDPERPGIVHRLDKLTTGVMVAARNAHAHAVLSRAFKAHE
                     HTRIYLALCWGSPGKDSGTIETFMQRNPKDRTRMTSRAKEGRRAVTHWEVLKRWRDFA
                     LLRLKLETGRTHQIRVHLAESGHPVAGDPVYGPRRRARTIPDPRVRSALEALSRQMLH
                     ASLLGIRHPATGEYLEFTSEPPEDMRAFMSALDAAQEGS"
BASE COUNT         1076 a         2198 c         2025 g         1205 t
ORIGIN      
        1 taatcggtca cataggttcc gctctggggc agggtcctca ccaggccgat ctcgctcagg
       61 tgcaccacgg cctccctcac ggccgccctg ctggcctcga accgccgcgc gagctcccgc
      121 tcggaaggaa gccggctgtt ggggggatac tcgcccctga agatcgcctt ctgcagctca
      181 aggcagatcc gttccgacac cttcactccc acccaagtcc ctccacacac gtggtgtacc
      241 cgatagggcg cgggtcgtca acgtcacgct ccctgcctcc cggccgcggc ctcccgcgac
      301 ggtttgcacc cgcagccacc acgggccctg tcatacacga gccagcagga aagtggagaa
      361 atcccggcgg gccgttcacc atggcatgta cgtcgggacc agccatacgc gagtcaagag
      421 gaaagtggag aaaactcgat ggagacccac acccgttgac gcctgaggcg cagtcacacc
      481 tttggggttt tctctcctcg agggatgacg gttgtggggc gattgtcatt ctgatcctgt
      541 tgaacgggaa gatggacctc gtccaggccg aggcatgacg gtttttgggg gttgtcattc
      601 tgattctgtg gttgacagaa gtattctttt tctccagact ggagatgtct ttaccatact
      661 tttcctcacc accgaggctt gcatggccga ccatccatgt tgtccttcgt gcggcagaag
      721 ggcgaagaag gctctgacga gcaaccactt tcccgtgtac cgctgcaggt cgtgcggaag
      781 ggtcttctgc atggagtgtt cgaacggcag ctgcccctcg tgcgggtcga actcgaggga
      841 caaggccgga aacgtctacg cgaggtgaca tgaccatgac gatctggcgt tccctatcga
      901 gattcttcca cccggcagac gcggctttca caactaccca taaaggaggt tcaggttcca
      961 tgaccaagag cgagctcgcg gcccgataca cggacttctt gacggaccag gggttcagac
     1021 ccgagatcga caaggacggc gacgtcaagt tcctcttcga gggcggcctg tactacatcc
     1081 tcgtctccga gaaggacccc gagttcttca ggctcgtcta ccccaacttc tgggagatag
     1141 agagcgacga ggaaaagatg aaggccacca tcgcggcctc gttcgccacg gccgacacga
     1201 aggtcgccaa ggtgatcctg gtaggcaaca acgtgtgggc gtcggtcgag tccttcttcg
     1261 agtcgccgga ccacttcaag ggtgtgttca ggcggtccat cagcgccatc aggaccgcgg
     1321 tctcctcctt ccgcgacaac atgaagcagc tctccggcgt gtaggcttaa acgggcgttc
     1381 acctcgcacc ccgaacgcat ggccgctctc gcccatgggc tggagacgcc ctgggcgagc
     1441 acctgcagcc gagagtccgg gcaagcttcg aggtgaggct tcccaaggcc tcaccgtgcc
     1501 catgggcaca cggggcaggt actcctcctt cgcgacgccc ttttccttgt ccccgggcgg
     1561 aaccgggccc tgcttccttg cgcgagcagg cccggcaggc cgacgccgct agcggtcacg
     1621 accggccgat gtcgatcctc acgggaggcg acgcccgccg tgcttctccc tcgggggacc
     1681 ttgagtttgg gcctgccgtg tggcatgatg catcttcatg gcacgttctt ggtataccct
     1741 gtacaggtcg gtggcgctct ccttcctcct cgccgtctgc gcctgcagtt cgtccggggg
     1801 cggggacgag ctgccgggga gtggtgagca tgtcgtcacc tacaaggtgg agggcggctc
     1861 gggcatcatc acgagcgcgt ccatctccta caccgacgag aacggggagg aacagcacct
     1921 ccagcacgtg agcctgaggg acgccaactg gtcctacagc ttcagggcgg acgacttcgc
     1981 ccccctgtcc gtaagtgcga tgctcgaggg cgagggcacg agcacgaccc tgtacgtgac
     2041 catagaggtg gacgacgcct gctgggtaga gaaccggacg ttcgttgcag gcgcgctgct
     2101 cactgtctct tccaccgcgc acgagatcct gaccacctgc ccgaaccacg gcgggaccac
     2161 ggtgcagtag gaggaccatc ccatgcccgc tgcacaggcg tcaccgaagg ggcagcgcac
     2221 tgcagggcga aaggcaggcc ggaaggtatc cacggcgcgc aaggacgaga tcatcgacgc
     2281 ggcggagcgc gtgttcgcat ccaagccctt ccccagggtg agcatgcgcg acatcgccag
     2341 ggaggccggc atctcgcctg cgctcatcta tcgccacttc gccgaccagc agcacctctt
     2401 cctcgaggcg ttcttcaggg gcgccgagcg tctcatgggc cgcatcgacg agctcttcaa
     2461 ggtgcacccg gagtccggcc tcgaggacgc ctcggccgcg ttcatctcct ttctcgccga
     2521 gaacgagcac tacttcaaga tgatgaccca cttcatgctc gaggggaccg tgcacgaggg
     2581 cctgctcgag aggctgaact ccatcgagcg ctcggtgctc gaccgcttcg acgagctctt
     2641 ccgcaaggcc ggggccgttg gggacgtccg catcctgtcc cactgtttct tcgcatccct
     2701 caacggcatc ctcatcacgt tcaggcgtca cccgggcagg accaaggaag aggtggtctc
     2761 ccacatgcac cgccttggaa tcaccctggc gcggctgttc gcacacgccc tgaggaccgc
     2821 aaacgtcggc ccgtgaagca cacggggatg ccggcccctg tgggggattc cttccatgcc
     2881 ccctgcaaag gggccaaaag ccggcctcga tctgcatccg ggtgcagcca ttgcccggaa
     2941 cggggcccta gcacctacga caacacctct gcgagcgcaa ggtcctggta ggcgtggaag
     3001 agcctgtggc acaggctgtc ccacctcctg cacacgtcct tcgtcttgtc gtccatggtg
     3061 aacaggctcg ggtgcgaggt gagcagctcc atctcggcgg ccagttcttc cacgaggcct
     3121 gtgtccgcgc ctttgaggat gtcccgcacg aacgcgccgg gcgcgtacca tggggcggcg
     3181 cgctggaagt ccctgtgcat ctgggtcagg agcacgttcc tcgggccttc ccagagctcg
     3241 ttgatcgccg cgtccctgaa catcctgggg agagaggaga aatcctccat cacgccgtgc
     3301 ccgccgaaga cggacatggc catgcgggcg acgtcggtcg cgtcccagct cgcggtgatc
     3361 ttctggagca tgatgagctc tctcacgcag aaccgcctct ttctcatgga gaggtcctcg
     3421 tctgcgagaa ggccgccctt gagcccgccc tcgagcccga ggaactcccg gtacagcatg
     3481 aaggcgccgg cggtggtgcg cttggcagag gtgtccatcc actcgagctg gtttgcgagc
     3541 atggggaaat gctcgagcct cactccgaag gcatccctga acgaggcgta cgctttcgcc
     3601 tcgcgcgccc ccctggtcat gcccgccgcg cccgagaggc ccacggtgag cctggagtac
     3661 gtgagcacga tgcccaccac gttcgcgacc cccctgtcca gggggcccac agggtaggcc
     3721 ttcgccccgt tgtacgtgat ctccgcggtg gtcagctcgc tcgtgcccat cttcaacttg
     3781 atacggtcga tggtgcagcc gttgcggatc tccttctcct tgtcaccctc gagccacgag
     3841 ggcaccacga agagcgccac cttctccgat cccgccggct tggcggtcac caccgcgtag
     3901 tcggcgtgcg tggccgaaca gaagaacttc ttcccgtaga gcctccaggt gccgttctcc
     3961 tccaccgctt ccagcaggtt cgcggggacg tcggagccgc cctggatctc ggagaggtac
     4021 tgggcgccga tgccgaagtc gccgtctatc ccctcgctgc agtgcatgag gatgcgcctc
     4081 gtctcggggg tgtcggcgaa gcgctcgagg agcgccacga gcccctcggt gcaggtcagc
     4141 gggcacgcga tgcacgcctc gccgttctgg tagatgaggt acatcttggc gagcttcagc
     4201 cacgggttcg tgcgtttcga gaagagggcc tcggagaaga cctccttctc catgacgagc
     4261 acctccatgg gcctcacgat gcggtcgatg cggtggttgt gcccgtcgta gtgcacgacg
     4321 tacggcctct tctcgggcac ggccgcggcc tcggccaagt cgcgccaccg gtaggagacc
     4381 ttcccggaaa acgctttcag ctcctcgtgg atcgcgggga agtcgtcgcc cgcatagcgc
     4441 ctgagcaccc gctggaagaa ctcgtcgtcg aggtagtagt tgaagcgtct cctccactcc
     4501 agaaacgcat cgaacgtgta cgggttgttc ctgtcaggca acgacatggc tctacctcct
     4561 tcatcgtcgt ctctcaagcc gcgctcggct gcgcacccct tgtatacacc cacaggccga
     4621 aaatgtaaac atcgtttaca tagtgacacc ccgctcctgg gttaggctgg gccttgctct
     4681 gggaacggcc tcggtgttcc ggtcacccca ctctgcacgt tgggacatcc cggacgagcg
     4741 gatcaaaggg acgactcatg agaacacccc cttccttccc aaggggtgct gcctgaactc
     4801 ctgccgcaat cgttcctggc cgcaggaggc agatgggtct gttcgcgagc cttgcgggaa
     4861 agggtcaaac ccctggccgt gccctgcccg gaggcattgt ctcggcctca cgcgtcattc
     4921 tcgcgtccgg cccgggccag ctgcctgagg agatccgcaa gcttggggtc tctcaccttc
     4981 tcgatgtccg acgggtctat atccaccttg aacggcgcgg tgcggggctc aggggggcgc
     5041 tgcggcctgg gccttcccgg tttcccgaag gaggcctcga cccccgagag cttgaggcca
     5101 acgaccccct tcacgcgctc gacgatgttg cctgcgagca ggcccacctg ctgggcccat
     5161 gcgggcgagt cgcacaggac gtgcagcttc gacgatttta tgagcacggg ctcggtgtgc
     5221 gacgcgagga cctcccccgc tatctcggcc cagcgggacc ggatcttcat gaccctggcc
     5281 atgttcttgt ccgcccgagc cagcgcctcg ccgatcgtct tcatgcggcc atattcgacc
     5341 acgcccatgg ggttgtcaag ggatccccgc ctttcgccct ctttccctgg gaacggccct
     5401 atcatcccgg tccatcaccc gcccttccct cccgccgcca cacctaatgt ctcattcggc
     5461 tgacggggat cacccgacgg tgaaacgatg ccgtatcccc cctgccccgt cacctgcctg
     5521 caggcgggag aggacgtccc tgctcacgat ccctcttgcg ccgcatcgag ggcggacatg
     5581 aacgctctca tgtcctcggg gggctcgctc gtgaattcca ggtattcgcc cgtggccggg
     5641 tgacggatgc cgaggaggga tgcgtgcagc atctggcggg agagcgcctc gagggcggag
     5701 cggacccggg ggtctggtat ggtcctcgcc ctgcgcctgg gaccgtagac cgggtcgccg
     5761 gcgacggggt gcccgctctc ggcgaggtgc accctgatct ggtgcgtgcg gccggtctcg
     5821 agcttcagcc tcaggagggc gaagtccctc cagcgcttga ggacctccca gtgggtcacc
     5881 gcgcgtctgc cctctttcgc cctcgatgtc atgcgggtgc ggtctttggg gttgcgttgc
     5941 atgaacgtct cgatggtccc cgagtccttg cctggagacc cccagcagag ggcgaggtag
     6001 atgcgcgtgt gctcgtgcgc cttgaacgcc ctcgagagga cggcgtgcgc atgggcgttc
     6061 ctggccgcga ccatgacgcc ggtggtgagc ttgtcgagcc tgtgcacgat gccgggcctt
     6121 tcagggtctc cgacgagggc catttccggg taccgggcga tgagcgcgcc tgcgagcgtc
     6181 ggcccggagg cgcccgcgcc cgggtgcacg ctcatgcccg cgggcttgtt gaccaccact
     6241 atccactcgt cttcgtagag gacctcgagg gggatcccca ccggctcgac aggcccggcc
     6301 tgcctgggag cggggcaggc gccctgcacc ctcatgccct gtttgagcct gaacgagggc
     6361 ttccgcgcca ggccgtcgac cctgatgcgc ccctgctcca cggcgcgtgc ggcaggcccc
     6421 ctgccgacgc cgatcgcccc tgccacgaac gcgtcgagcc tgatgccctc cccctcgccg
     6481 ctcacgagca tatcaatggg caag
//
LOCUS       DAOSCR010000066         6473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7623_length_6473_cov_3.687286, whole genome shotgun sequence.
ACCESSION   DAOSCR010000066 DAOSCR010000000
VERSION     DAOSCR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7623_length_6473_cov_3.687286"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2848
                     /locus_tag="PLT69_02865"
     CDS             <1..2848
                     /locus_tag="PLT69_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13417.1"
                     /translation="SGDACFAVDRSLVVVAVSPANKTPIAATIADLEGRRLDAAGVIH
                     PDDVGRALRDIEALLAGGGICGAVYRFFCRDGPLCAGEVWGFPFMEGGRVACGLCVVR
                     CVSVTDHTRSGRKQRLKDIVAPLEAGGIEERVYRFMVEESLEEVYLVDDDAVVVYANA
                     QAASSLGYTPNEMMSMQVADFDPVYGPRFREVFEEARRGRSMFETVHVTRQGSYLDKE
                     IACRYLVIDGQEYICGFGRDVTQKKKVERELRESEKRFRDLVENTSDLIWETDEELRF
                     SFISRTEERHLGYAPGDMIGKTLLDFVPPDQAPAVESTLRDLKENPRPFTNLVYTALH
                     KDGSLHIRQTSGVPVYDDHGRFAGYRGVTRDITEQKRVEELLVASEKKYSAVFGASPV
                     PLVLSRFSDGLLLDWNASFERWSGHTREDLAGRTSTEVGLWEDPCDRGRLVGELAEAH
                     CLDSWETIFRLRDNTVRDVVLASCLIELEGEGVMLTQIQDVTDRKRIEDELRRSRQAF
                     LVAFHESPAPMVITRLADGVVLDANRACLSWSGFERDEVVGASTVGLGFWTDSAARTA
                     FMERVRSHGRIDSEETTYRVRDGSLRQVIHSARLLDLFGVPCILSHILDVTEARRASL
                     SLRESEALLRGIVDNFPGAVFRFYVKDQGERGLHYLSASTLDVLGIPNEREGFLEHVL
                     ECLHPEDRCRFEASVEEAVRTMSAWDFTTRFKRPSGEWIVVRGISRPERLEGEVVFSG
                     VILDVTEQHRLSEELASHRDHLEETVRERTKELTDALARLTSEIETRKKTESMLRYRE
                     MEIENRRLELEEMNAALRVLIKQREEDKATMEENIKGNIRTFVLPHVERMESSGLTGG
                     PRQSLEMIRSYLMDITSSFGRRVSSETLGLTPVEIQVASLIRQGKTSKEIARILNVSL
                     NTVQTHRYHIRKKGRLGNKKVNLRTYLKGLERKD"
     gene            3394..3615
                     /locus_tag="PLT69_02870"
     CDS             3394..3615
                     /locus_tag="PLT69_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13418.1"
                     /translation="MAAKPDRHLSLASENRIHRLVDFTCHIQEGDELVIARLSSMLGK
                     SPSDLITEAIRGLIRKYSTRRVPLRVVRR"
     gene            4105..4488
                     /locus_tag="PLT69_02875"
     CDS             4105..4488
                     /locus_tag="PLT69_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOJ13419.1"
                     /translation="MSAPEHLHVHPCLRCGACCAYYRASFYWAEADDATPGGVPVGMT
                     RRLTEHRRSMLGMDGDRPRCVALVGEVGGRVYCSIYERRSSVCREFEPSFENGSANER
                     CDRARAAWGLRPLTPDDWASWRSEG"
     gene            4975..>6473
                     /locus_tag="PLT69_02880"
     CDS             4975..>6473
                     /locus_tag="PLT69_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ13420.1"
                     /translation="MNKGFKLSTKVTIVVLAIGGIVNVSTIAMCLYSFRGELERLALQ
                     NQEMRQKVFWELLRSKGGTFEVVGDNLVVGGDYVVNGNYELPDRLKELCGGTATIFMQ
                     DTRVSTNVLKEDGTRAVGTKLKGVAYDAIFREGKPYRGKAEIMGQTYFTAYDPITDAT
                     GKVIGVLYTGVKRSEFFSSFDSLLVNLLVVIGLLVTLSALALPFIMKFMLGVPLMELV
                     RSIGSLAEGGGDLTRRVTVNRRDEIGMLAAELNRFLGSLERMVAKVKDESEMIEATSR
                     DVAEGTKGLNQMTQEQASAVEEVASTVEEMTSAIKQNASNAEQGRSKARSMVQTAEAS
                     MKASLDLAAAMAEISGASKKISDIITTVNEVAFQTNLLALNAAVEAARAGEHGKGFAV
                     VAEEVRSLAQRSADAARQIRVLIEDTVGKVKAGDEIMKRSSESLHEMMGYINDIMQSI
                     EEIAASSAQQASGVDELNRAIAQIDMTTQRNAATVEQIAAAAEQMKDDSL"
BASE COUNT         1286 a         1892 c         2177 g         1118 t
ORIGIN      
        1 ctcgggagat gcgtgcttcg cggtggacag gtccctcgtg gtggtcgccg tctcccccgc
       61 caacaagaca ccgattgcgg ctaccatcgc ggatctcgag ggaaggcgtc tcgatgcggc
      121 aggggtgata caccccgacg atgtcggccg ggccctccgg gatatcgagg ccctgctcgc
      181 aggcggtggg atctgcggcg ccgtgtaccg cttcttctgt cgcgacggtc ccctgtgcgc
      241 cggggaggtc tgggggtttc ccttcatgga gggaggcagg gtggcctgcg gcctctgtgt
      301 tgtcaggtgc gtgagcgtga ccgaccacac gcggtcgggg cgaaagcagc gtcttaagga
      361 catcgtcgcg cccctcgagg ccggggggat cgaggagcgg gtctacaggt tcatggtgga
      421 agagtcgctc gaggaggtct acctcgtcga tgacgacgcc gtggtcgtct atgcgaacgc
      481 ccaggcggcc tcgagcctcg ggtacacccc caacgagatg atgtccatgc aggtggccga
      541 tttcgatccc gtctatgggc cgcgcttcag agaggtcttc gaagaggcga ggcggggcag
      601 gtcgatgttc gagacggtgc atgtcacgag acagggaagc tatctcgaca aggagatcgc
      661 gtgcaggtat ctcgtcatcg acggccagga atacatctgc ggcttcggca gggacgtcac
      721 ccagaagaag aaggtggagc gtgagctcag ggagagcgag aagaggttca gggacctggt
      781 ggagaacacg agcgacctga tctgggagac agacgaagag ctcaggtttt ccttcatcag
      841 ccggaccgaa gagcggcacc tcggatatgc gcccggggac atgatcggca agaccctcct
      901 cgacttcgtc ccccccgacc aggccccggc cgtcgagtcg acgctcaggg atctcaagga
      961 gaaccccagg ccgtttacca acctcgtata cacggccctc cacaaggatg gttccctgca
     1021 catccggcag accagcggcg tcccggtata cgacgatcac ggccggttcg cggggtaccg
     1081 gggggtcacg agggacatca cggagcagaa gagggtcgag gaactcctcg tcgcctccga
     1141 gaagaagtat tccgccgtgt tcggggcgag cccggtcccg ctggtgcttt cccggttctc
     1201 agacgggctc ctgctcgact ggaacgcgtc gttcgagagg tggtccggcc acacccggga
     1261 ggatctcgct gggaggacat cgaccgaggt gggtctctgg gaggacccct gtgaccgcgg
     1321 gcgtctcgtg ggagagctcg cagaggccca ttgtctcgat tcgtgggaga cgatattccg
     1381 cctgagagac aacacggtga gggatgtcgt gttggcttcc tgtctcatcg agctcgaggg
     1441 cgagggggtg atgctcaccc agatacagga cgtgaccgac cgcaagagga tcgaggacga
     1501 gctcaggaga tctcggcagg cgttcctggt ggccttccac gagagccccg cgcccatggt
     1561 gatcacgagg cttgcagacg gcgtcgtcct ggacgcgaac agggcgtgcc tgagctggtc
     1621 cggcttcgag agggacgagg tcgtgggcgc aagcacggtg gggctcggat tctggaccga
     1681 cagcgccgca cggacggcat tcatggagcg ggtgaggtcc cacggccgga tcgattcgga
     1741 ggagacgacc tatagggtca gggacgggag tctcaggcag gtgatccact cggcccgcct
     1801 cctggatctc ttcggcgtcc cgtgcatcct ctcccacatc ctcgatgtca ccgaggccag
     1861 gagggcctcg ctctccctca gggagagcga ggccctcctg aggggtatcg tggacaactt
     1921 cccgggcgcg gtgttcaggt tctacgtgaa agaccaagga gagaggggcc tccactacct
     1981 gagcgccagc accctggatg tcctgggcat accgaacgaa agagaggggt tcctcgagca
     2041 cgtgctcgag tgcctgcacc cagaggacag gtgccgcttc gaagcctctg tcgaggaagc
     2101 ggtgaggacc atgagtgcat gggacttcac caccaggttc aagaggccct cgggcgaatg
     2161 gatcgtggtg cgcgggatct ccaggccgga gcgcctggag ggcgaggtcg tcttcagcgg
     2221 cgtgatcctc gacgtcaccg agcagcacag gctcagcgag gagctcgcct cccacaggga
     2281 ccacctcgaa gagacggtgc gagagaggac caaagagttg accgatgccc tcgcgaggct
     2341 caccagcgag atcgagacga ggaagaagac cgagtccatg ctccgctacc gcgagatgga
     2401 gatagaaaac cgaaggctcg agctcgagga gatgaacgcc gcgctcaggg tccttatcaa
     2461 gcagcgcgag gaagacaagg cgaccatgga ggagaatatc aagggaaaca tccgcacgtt
     2521 cgtgctcccc catgtcgagc gcatggaatc ctccggcctg accggcggac ccaggcagtc
     2581 cctcgagatg atcaggtcgt acctgatgga tatcacgtca tccttcggca ggagggtgtc
     2641 ctcggagaca ctgggtctca cgcccgtgga gatccaggtg gcctcgctca tcaggcaggg
     2701 aaagacgtcc aaggagatcg cgaggatcct gaacgtatcc ctgaacaccg tgcagaccca
     2761 ccggtaccac atcaggaaaa aggggaggct gggcaacaag aaggtgaacc tgaggacata
     2821 cctcaagggt ctcgaaagga aggactgacg gcgcccgacc accgatgcgg ccggtgccgc
     2881 ggcccctttg gcagggcttt cgggtctgga aaaaggagcg gctctcctgc aggccgagta
     2941 tcgcccctta agcccaagcc tgtctcttct tgctaaggcg cagtcggggc gcgcgtcttt
     3001 cgccgtgaga ctcgaccctc gtgacgctga aatcggtacc cggtgggggc tgtccgtaaa
     3061 ggcgtcatgt ggctcgtggc aacaacaagc tgttgtctgt ccagggtcag cccgaacgtc
     3121 accgtgaacg ctcgtgtctg ctcgtgtcgg caagatgggg tggggtcggg tggatgacgg
     3181 ggtcgcctgc gcccccttgc ggccgccctc caggggtcat gcggtgatga tgaaagataa
     3241 ggagggtcat ctatgataaa acatcaccat tctatcaccg atggatcatc atcgctcccc
     3301 ggtgtgcctt ttgttatgaa tgtgttgaga gaattccacc gtgatccctg gcgcagagtt
     3361 cacacgaggg cttgcgggca ccaagggggc gggatggcag caaaaccaga caggcacctg
     3421 agtttggcat cagagaacag gatccacagg ctcgtggact tcacctgcca catccaggag
     3481 ggtgacgagc tcgtgatcgc ccggttgtcc tcgatgctcg gcaagtcccc ctcggacctg
     3541 atcaccgagg cgatacgcgg cctcataagg aaatactcca cccggagggt ccccctgagg
     3601 gtggtccggc gctgaacagc ctggggcgcc ccttggcggc ccaccacgtt cacccaggct
     3661 gcctgggccc gggaagcctg gaaaaaccca cccggatcaa gggccttccc gctcgtcgat
     3721 gccgctcgat gccgggtttc cgcctctcgg tatccatgaa taccatgatg gggtgtcagt
     3781 cgtgacgcgc cgtgcgcaca agcggcgtgt ccctcgcctg tgtgaccccc tggcctgggt
     3841 ggtcgatgtc caagacccgg gcatcatgag ctccgaggaa ctgctcgtcg ttctggcatc
     3901 tggcgccggg taatcaggcg atagccatgt ttgcaagcag tgaaccgggg aaccgctcgg
     3961 cggcatgaca tcgggcgcct tcgggaaggg tcgccgtctc gtcacgacga tcatgccccg
     4021 catccgaccc cttctccccc cagtcctcgg agctcttgca ttttcaaccc gagtcagtca
     4081 tatatcctga tccttgaggt atccatgagc gcaccagaac accttcacgt ccatccatgc
     4141 ctgaggtgcg gggcctgctg cgcctactac cgagcctcgt tctactgggc cgaggcggac
     4201 gacgccacgc cgggaggggt ccctgtcggg atgaccagga gactcaccga gcacaggcgc
     4261 tccatgctcg gcatggacgg agacaggccg aggtgcgtcg ccctcgtagg ggaggtgggc
     4321 gggcgggtgt actgttccat ctacgagagg cgctcgtcgg tgtgcagaga attcgagccg
     4381 tcctttgaga acgggtcggc aaacgagcgg tgtgaccggg cacgcgccgc gtggggactc
     4441 aggccgctca ctcccgacga ctgggcctcg tggcgcagcg aagggtgatc acacctccgt
     4501 cacggcgaca tgccgcaccc ttcttcacca cggcgcgata cattgcagaa tcacgcccgt
     4561 agcgggttct gtcacgtcaa catgccgcac ccctctctac caccgggggc atgctcgaga
     4621 tgatcgggtg aggatgcggc gtcaccctct gtcaagcagc gatcctgtca ctcagccacc
     4681 tcccttcggc gatccattca ccacggcagt gtgcggcaat cctcgttatc gtcgtctttt
     4741 ggcgccgttt cgggttgaca gggtgtttta cgctttcctg cgggaggatt tccgggtttc
     4801 cttcaaggtt tgatcgccga atgccgatat gccgggccga gggtgaacgc aggacagagg
     4861 cactaaagaa aggtgccgtc attgcaatgg tgaggcccct tacggccatg tcaagacaag
     4921 gggccggatg cgtggggctg atagtgaaaa agctataggg ttgggagggt tgcaatgaac
     4981 aagggtttca agttgtccac gaaggttacc attgtcgttc tcgcaatcgg cggtatcgtg
     5041 aacgtgagca cgatcgccat gtgcctttac agtttccggg gggaactcga gaggctggcc
     5101 ctgcagaacc aggagatgag gcagaaggtc ttctgggaac tcctgcgctc caagggcggc
     5161 acgttcgagg tcgttgggga caacctcgtc gtcgggggcg actacgtggt caacggcaac
     5221 tacgagctcc ccgacaggct caaggaactc tgcgggggca ccgccacgat cttcatgcag
     5281 gacacgaggg tctcgacgaa cgtgctgaag gaggacggga ccagggcggt ggggaccaag
     5341 ctcaaggggg tggcctacga tgcgatcttc agggaaggca agccgtaccg cggcaaggcc
     5401 gagatcatgg gccagaccta cttcaccgca tacgatccca tcacggatgc gacagggaag
     5461 gtcatcggcg tgctctatac cggcgtgaag cggagcgagt tcttctcctc cttcgactcc
     5521 ctcctcgtga acctcctcgt ggtcatcggc ctgctggtca cgctgagtgc attggcgctc
     5581 ccgttcatca tgaagttcat gctcggcgtt cctctcatgg agctcgtcag gagcataggg
     5641 agcctcgctg agggaggcgg agacctcacc aggagggtca cggtgaacag aagggacgag
     5701 ataggcatgt tggccgcgga actgaacagg ttcttgggaa gcctcgagcg tatggtcgcc
     5761 aaggtgaagg acgagtcgga gatgatagaa gcgacctccc gggatgtcgc cgaggggacc
     5821 aaggggctca accagatgac ccaggagcag gcgagcgcgg tggaggaggt ggcctcgacc
     5881 gtcgaggaga tgacctcggc catcaagcag aacgcgagca acgcggagca gggacgcagc
     5941 aaggcgcgct ccatggtgca gaccgcggag gccagcatga aggcgtccct tgacctggcc
     6001 gcggccatgg cggagatctc gggcgcatcc aagaagatat cggacatcat caccacggtg
     6061 aacgaggtgg ccttccagac gaacctgctg gccctgaacg cggcggtgga ggcggcccgg
     6121 gcgggggagc acggcaaggg cttcgccgtg gtggccgagg aggtgcggtc gctcgcgcag
     6181 cgttcggccg atgccgcgag gcagataaga gtactcatcg aggatacggt ggggaaggtc
     6241 aaggccgggg acgagatcat gaagagatcg tccgagtcgc tgcacgagat gatggggtac
     6301 atcaacgaca tcatgcagtc gatcgaggag atcgccgcct cgagcgccca gcaggcttcc
     6361 ggcgtggacg agctcaaccg tgccatagcg cagatcgaca tgacgaccca gaggaatgcc
     6421 gcaacagtgg aacagatcgc cgccgccgca gaacagatga aggacgactc cct
//
LOCUS       DAOSCR010000067         6359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7798_length_6359_cov_3.409264, whole genome shotgun sequence.
ACCESSION   DAOSCR010000067 DAOSCR010000000
VERSION     DAOSCR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7798_length_6359_cov_3.409264"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PLT69_02885"
     CDS             complement(<1..1011)
                     /locus_tag="PLT69_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13421.1"
                     /translation="MARRSLNGNPYRFQLTIKDLLHSPRTQALRQEIVYRDRVRYTYA
                     DLLDRIGRLASGLAALGVRQGDTVAVFDYDSHRYLECFFAVPMMGAVLFMVNWRLSPE
                     QIEYTINHAQADVVLINADFLPLFSSIRGTLSTVRKVVVMSDRDEPHAADFPVDAEYE
                     PMLSSSSPDYDFPDLDENTKATTFYTTGTTGLPKGVFFSHRQIVLHTLSVCITVSCHD
                     TPLRFSTSDVYMPITPMFHVHAWGFPYIATMMGIKQVYPGKYEPETLVRLILKERVTF
                     SDCVPTILQMLVNSPIAKTVDLSSWKVVIGGARLPKGLAKAALDLGIQVMAGYGMSET
                     CPV"
     gene            complement(1386..2072)
                     /locus_tag="PLT69_02890"
     CDS             complement(1386..2072)
                     /locus_tag="PLT69_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ13422.1"
                     /translation="MKTPRRGPGHRYRILVVDDHPIFRHGLAQLISQEDDLEVCGEAE
                     DYHDALKAASELLPDLIVVDITLKETSGIELIKEIHRRFKEMPMLVISMHDETLYAER
                     TLRAGARGYIMKQEASENVISAIRKVLSGGIYTSHRITDDIMRRFLEGTSEPLESPLN
                     RLTDRELEIFRLIGEGLSISEIGRRLHLSVKTVGTHRERIKEKLNLKSATELLRYALH
                     WVEQERVSGS"
     gene            complement(2059..3183)
                     /locus_tag="PLT69_02895"
     CDS             complement(2059..3183)
                     /locus_tag="PLT69_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13423.1"
                     /translation="MKDSTDDVRRLEAELASLRSEVRRLEEQARRYLRIEAELIQSEN
                     FYRTLFENSGTATILIEEDTTVSMLNSDFANFTGYSRDEVLGKSWTHYVAEDDIPRLL
                     EYHRLRRIDPRAAPRNYEFKIRNKDGGLLHIYMTISMVPGTKQSIASMVDITALKKLE
                     REVLDIGERERLKIGYALHDDLGQHLIGIEFMTKVLTNRLAAVSPEDASYASEINALV
                     KEAIAKTRMLARGLCPVDLTSLGLESALEELARSTSNIFSISCTFSCPRHVPIRDNTL
                     STHLYYIAKEAVHNAIEHGQASKVAIELLNSSGTVSLSVLDNGMGMTDPRQADGMGIK
                     IMNHRARMIGATLTIESEPSGGTLVTCTFTQEGTEGNEDS"
     gene            complement(3265..4065)
                     /gene="hisF"
                     /locus_tag="PLT69_02900"
     CDS             complement(3265..4065)
                     /gene="hisF"
                     /locus_tag="PLT69_02900"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ13424.1"
                     /translation="MLSKRIIVCLDVKGGKTTKGVRFLDNVDIGDPVEMAREYYLQGA
                     DELVFYDITASSESRDIMIDVVRSVAANIFIPFSVGGGIRTLDDMYRVLDAGAEKISV
                     NSAAVLNPEIISEGARRFGSQCIVLGMDVLRVGPSEKIPSAYEVYINGGRTPMGIDAL
                     AWARQAQDLGAGEICLNSIDADGTNDGYELTVTRLVSSGVTIPVIASGGAGRPEHIRD
                     VLVEAGADAALIASMVHYGRYTVGGIKELLKDNGVPVRLASDPRRAAS"
     gene            complement(4290..6149)
                     /locus_tag="PLT69_02905"
     CDS             complement(4290..6149)
                     /locus_tag="PLT69_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOJ13425.1"
                     /translation="MASLIVDERDQRFALNEVLEIGKLCEFERYADFSVDMFDMILTE
                     AQKYAEEEIFPTLAESDKVGCRLEGGQVYVPQCFHKPYKLFCEAGWNCMSFPQESGGQ
                     GLPFILRIAAHEWFIHNFAFACYPGLAEGAAHLIEVYGTQEQKKKYLPKMVSGVWGGS
                     MCLTEPGAGSDVGNLSTKAVRQPDGTFKIQGTKIFITGGDQDLTENIIHPVLARIEGD
                     PAGTKGISIFLVPKYLVNDDGTLGRRNDYEIAKIEEKMGIHGSSTCLINFGDNNECYA
                     ELLGGEREGMKIMFQMMNEARIATGLQGLGSASVAYLHALKYTKERLQGSSLLEFKNP
                     DAPRVPIIQHPDVRRMLLWMKSNVEGMRALMYYATVCGDRAASSKDPAEAEKWLGIVE
                     VLTPIVKAYCTDMGFRVIETAMQCYGGYGYCSEYPIEQFMRDEKIATIYEGTNGIQAL
                     DLVGRKMGMKKGAYFMTLLGEMGATVSKYQGVEQLKDLALDVQAGVNTLAEIGMFFAS
                     CGKSGEYLVPVGNAYPFLMMMGKIVCAWLLLWQAGVAREKLEAICRAKGVSMTDASAL
                     ASLFKDNKDAAFYEGKIKAARYFIKNVLPEVEAAAKAIKSKDLSMMEIPDEGF"
BASE COUNT         1005 a         2025 c         2023 g         1306 t
ORIGIN      
        1 caccgggcag gtctcggaca tgccgtagcc ggccatgacc tgtatgccga ggtcgagcgc
       61 ggccttggcc agccccttgg ggagcctcgc gcccccgatc accaccttcc aggacgagag
      121 gtcgacggtc ttggcgatcg ggctgttcac gagcatctgg aggatggtcg gcacgcagtc
      181 cgagaaggtc acccgctcct tgaggatgag cctgacgagg gtttccggtt cgtacttgcc
      241 cgggtagacc tgcttgatgc ccatcatggt cgcgatgtag gggaaccccc aggcgtggac
      301 gtggaacatg ggcgttatgg gcatgtagac gtccgacgtc gagaacctga gaggcgtgtc
      361 gtggcagctt accgtgatgc acacgctgag cgtgtggagc acgatctgcc ggtgcgagaa
      421 gaacacgccc ttgggcagcc ccgtcgtgcc cgtggtgtag aacgtcgtgg ccttcgtgtt
      481 ctcgtcgagg tcggggaagt cgtaatcggg ggacgacgag gagagcatgg gctcgtactc
      541 ggcatccacc gggaagtccg ccgcgtgcgg ctcgtccctg tcgctcatga ccaccacctt
      601 cctgaccgtg gacaacgtgc cgcggatgga ggagaagagg ggcaggaaat cggcgttgat
      661 cagcaccacg tcggcctgtg cgtggttgat cgtgtactct atctgctcgg gggagagcct
      721 ccagttcacc atgaagagca cggcgcccat catggggacc gcgaagaagc actcgaggta
      781 acggtgggag tcgtaatcga acaccgccac cgtgtccccc tgcctgaccc cgagggccgc
      841 cagcccgctt gccagcctgc ctatgcggtc gagcaggtcg gcgtaggtgt agcggaccct
      901 gtctcggtac acgatctcct gcctcaaggc ctgcgtcctc gggctgtgca gaaggtcctt
      961 gatggtaagc tggaaacggt acggattccc gttcaacgat ctcctcgcca tggctgcctc
     1021 ctttgtgaag agtctctcga taccggcatg atcggactct tcgcgaaagg agaacatcgg
     1081 catcgaaccg gtttttcagt aagaagatgg accaaagtgg cctcggtcga tgacctacct
     1141 cgaggagggg gctcatgccg tctcagggat gtccccgtcc atggccggca cgaggcggcg
     1201 ctcccccttc tctccgggcg ggcgtccccg ccgggcggac ccctcccgcg gccatggtcc
     1261 gtgagcctgt catgcggcgg gatccggcgg gtacggcaag gtccggacct cggccgggcc
     1321 gcaaggcgcc gcgccttcct gcatcccggc gcgaggcggg acccgccggg ttgcggcagg
     1381 ctcacctacg agcccgagac ccgctcctgt tccacccagt gcagggcgta gcgcaagagc
     1441 tcggtcgcgc tcttgaggtt cagcttctcc ttgatgcgct cccggtgggt gcccacggtc
     1501 ttcacgctca ggtgcagccg tctgccgatc tcgctgatgc tcaggccttc gcctatgagc
     1561 ctgaagatct cgagctccct gtccgtgagc cggttgagcg gggactcgag gggctccgag
     1621 gtcccctcga gaaagcgtct catgatgtcg tcggtgatgc ggtggctcgt gtaaatgccg
     1681 cccgagagga ccttccggat agcggatatc acgttctcgg atgcctcctg cttcatgatg
     1741 tatcccctgg ccccggccct cagcgtgcgt tccgcgtaga gcgtctcgtc gtgcatggat
     1801 atgacgagca tcggcatctc cttgaaccgc ctgtggatct ccttgatgag ctcgatgccg
     1861 ctcgtctcct tgagcgttat gtccacgacg atcagatccg gcaggagttc cgatgccgct
     1921 ttcaatgcgt cgtgatagtc ttcggcctcc ccgcacacct ccaggtcgtc ctcctgggag
     1981 atgagctggg ccaagccgtg gcggaatatc gggtggtcgt ccaccacgag gatcctgtac
     2041 ctgtggcccg ggccccttct aggagtcttc atttccctcg gtcccctcct gggtgaaggt
     2101 gcaggtcacg agcgtgccgc ccgatggttc cgattctatc gtgagcgtcg cgccgatcat
     2161 gcgtgccctg tggttcatga tcttgatccc catgccgtcg gcctgccttg ggtccgtcat
     2221 gcccatgccg ttgtcgagaa cggacagcga gacggtgccg ctgctgttca ggagctcgat
     2281 ggccaccttg ctcgcctggc cgtgttctat ggcgttgtgg acggcctcct tggcgatgta
     2341 gtagaggtgg gtggacaacg tgttgtccct gatcgggacg tgcctcgggc agctgaaggt
     2401 gcacgagatg gagaagatgt tcgaggtcga gcgggcgagc tcctcgaggg cggactcgag
     2461 gccgagcgag gtgagatcca ccgggcagag gcccctggcg agcatcctcg tcttcgcgat
     2521 cgcctccttg accagggcgt ttatctccga ggcgtacgag gcgtcctccg gggagactgc
     2581 cgcaagcctg ttcgtgagca ccttggtcat gaactctatg ccgatgaggt gctggcccag
     2641 gtcgtcgtgg agggcatagc ctatcttcag cctctccctc tcgccgatgt cgagcacctc
     2701 gcgctcgagc ttcttcaatg cggtgatgtc caccatggag gcgatgctct gcttcgtccc
     2761 ggggaccatg gagatggtca tgtagatgtg gagcaggccg ccgtccttgt tcctgatctt
     2821 gaactcgtag ttcctgggtg cggctcgagg gtctatcctg cgcaggcggt ggtattcgag
     2881 gagcctcggg atgtcgtcct cggccacgta gtgcgtccag ctcttcccga gcacctcgtc
     2941 ccgggaatag ccggtgaagt tcgcgaagtc cgagttcagc atggacacgg tggtgtcttc
     3001 ctcgatgagg atggtcgccg tcccggaatt ctcgaagagc gtgcggtaga agttctctga
     3061 ctggatgagc tcggcctcga tcctgaggta gcgtctcgcc tgttcctcga gccggcgcac
     3121 ctcgctcctg agagaggcga gctccgcctc gagccttctt acgtcgtcgg tagagtcttt
     3181 caccgtgccg gtaccccccg cgtcccggat cgttgtaccc gaagacaccc ccgcgtgtcg
     3241 aggcgcccgt gcgtcctcgc ggtgtcagga ggcggctcgg cgggggtcgg acgccagcct
     3301 cacggggacg ccgttgtcct tgaggagttc cttgatgccc cccaccgtgt acctgccgta
     3361 gtgcaccatg gacgcgatga gcgccgcatc cgcccccgct tccacgagga cgtccctgat
     3421 gtgctcgggt cttcccgcac cccccgaggc gatgaccggt atggtgacgc ccgaggacac
     3481 gagcctcgtc acggtgagct cgtagccgtc gttcgtcccg tcggcgtcga tggagttgag
     3541 acagatctcg ccggcgccca ggtcctgagc ctgcctggcc cacgcgagcg cgtcgatacc
     3601 catgggggtc ctgccgccgt tgatgtagac ctcgtaggcc gaggggatct tctccgatgg
     3661 gcccacgcgc aacacgtcca tgccgaggac gatgcactgg ctgccgaaac gcctggcgcc
     3721 ttcggagatg atctccgggt tcagcaccgc cgcgctgttg acgctgatct tctccgcccc
     3781 ggcatcgagg acgcggtaca tgtcgtcgag cgtcctgatg ccaccgccga cgctgaaggg
     3841 gatgaagatg ttcgcggcga ccgacctcac gacgtcgatc atgatgtccc tgctctccga
     3901 ggaggccgtg atgtcgtaga acacgagctc gtccgcgccc tggaggtagt actcccgtgc
     3961 catctccacg gggtcgccga tgtccacgtt gtcgaggaac cgtacgccct tggtggtctt
     4021 gccgcccttc acgtccaggc agacgatgat gcgcttgctg agcatgagaa cgttctagga
     4081 caaaacagga ctcgtatcca gagggatctt gccctggtgc cgaacggcag gcgcgatctc
     4141 gggtgggcct gggccgcatc cccggatcgg ggcaggggat gcgccggcgc gaccacctcc
     4201 ggggggttgg aaactcccgg gggcgtgccg tctcggggag ttccccacgg cacgcccccg
     4261 gcgagaggcg tcgtcttttg ggcgttttcc tagaagccct cgtccgggat ctccatcatg
     4321 gagaggtcct tgctcttgat cgccttggcg gcggcctcca cctcgggcag cacgttcttg
     4381 atgaagtagc gcgcagcctt gatcttgccc tcgtagaacg ccgcgtcctt gttgtccttg
     4441 aacagggagg ccaaggccga tgcatccgtc atggacacgc ccttggcccg gcagatggcc
     4501 tcgagcttct cgcgcgccac gcccgcctgc cagagcagga gccatgcgca cacgatcttt
     4561 cccatcatca tgaggaaggg gtacgcgttg cccacgggca cgaggtattc gccggacttc
     4621 ccgcacgatg cgaagaacat cccgatctcg gcgagcgtgt tgacacccgc ctggacgtcc
     4681 aaggccaggt ccttcagctg ctcgacgccc tggtacttgg acacggtcgc gcccatctcg
     4741 ccgagcaggg tcatgaagta cgcgcccttc ttcatgccca tcttcctgcc caccaggtcc
     4801 agggcctgga tgccgttggt gccctcgtag atggtcgcga tcttctcgtc gcgcatgaac
     4861 tgctcgatgg ggtactcgct gcagtaaccg tacccgccgt agcactgcat ggccgtttcg
     4921 atgaccctga agcccatgtc cgtgcagtag gccttgacga tgggcgtgag cacctcgacg
     4981 atgccgagcc acttctccgc ctccgcggga tccttggagc tcgccgccct gtcgccgcag
     5041 acggtggcgt agtacatgag cgccctcatg ccctcgacgt tggacttcat ccagagcagc
     5101 atgcgcctga cgtcagggtg ctggatgatg gggactcggg gggcatccgg gttcttgaac
     5161 tcgagcaggg aagagccctg gagcctctcc ttcgtgtact tgagggcgtg caggtaggcg
     5221 acgctcgcgc tgccgaggcc ctggaggccc gtggcgatcc tggcctcgtt catcatctgg
     5281 aacatgatct tcatgccctc gcgctcaccc ccgagcagct cggcgtagca ttcattgttg
     5341 tcgccgaaat tgatcaggca ggtcgagctc ccgtgtatgc ccatcttctc ttcgatcttg
     5401 gcgatctcgt agtcgttgcg ccttcccagc gtgccgtcgt cgttgacgag gtacttgggg
     5461 acgaggaaga tggatatgcc cttggttccc gcagggtcgc cctcgatgcg tgcgagcacg
     5521 gggtggatga tgttctccgt caggtcctgg tcgccgcccg tgatgaatat cttggtgccc
     5581 tgtatcttga aggtgccgtc cggctgcctc acggccttgg tgctcaggtt gcccacgtcg
     5641 gatcccgcgc cgggctcggt gaggcacatg ctcccgcccc agacgccgga gaccatcttg
     5701 ggcaggtact tcttcttctg ctcctgtgtc ccgtacacct cgatgaggtg cgcggcgccc
     5761 tccgcgaggc ccgggtagca ggcgaaggcg aagttgtgga tgaaccattc gtgcgccgcg
     5821 atcctcagga tgaagggcag gccctgcccg ccggattcct gggggaacga catgcagttc
     5881 caacccgcct cgcagaagag cttgtagggt ttgtggaagc actggggcac gtatacctgc
     5941 ccaccctcga gcctgcaccc taccttgtcc gactcggcga gcgtcgggaa gatctcctcc
     6001 tcggcgtact tctgcgcctc ggtgaggatc atgtcgaaca tgtccaccga gaagtccgca
     6061 tacctctcga actcgcagag cttcccgatc tcgagcacct cgttgagcgc gaacctctgg
     6121 tccctttcgt cgacgatcaa gctggccatg catccctcct ctcggattgg tatcgaaact
     6181 cacacccgga atccctctcg gttcctggga cctcccggtg ggcttgccag gggctgtctg
     6241 tcgcccgtcc tcgtccgttt aatgtattac aatatgagat tcatacaaac caggtgaaac
     6301 ggaatcgtac ccgggaagcc tcgcgcttgt caagtcgcca agggtcctac acccggggg
//
LOCUS       DAOSCR010000068         6356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7801_length_6356_cov_3.398032, whole genome shotgun sequence.
ACCESSION   DAOSCR010000068 DAOSCR010000000
VERSION     DAOSCR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_6356_cov_3.398032"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLT69_02910"
     CDS             complement(<1..331)
                     /locus_tag="PLT69_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13426.1"
                     /translation="MKPETREYRYDWNIWTIMENAALVKDGTDKEYESLLGLIPHFAD
                     ALDIFIRQGEPGVRFIKLVANYIRACIEAKALGKKTVLTSFSMATPILYAFDLVPICL
                     EAFTIFGT"
     gene            complement(328..1482)
                     /locus_tag="PLT69_02915"
     CDS             complement(328..1482)
                     /locus_tag="PLT69_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ13427.1"
                     /translation="MIETLRKAARSIGNPSLQAWNEGGGKVLGYPCTFVPEEIIDAAG
                     ILPYRLRGITAGTMSIGDAYFGPVICSFPKCILQLAGQGSYGFLDGAVVVNGCDSMRR
                     LHECWRKASEDIPGTLPGFFHYMGVPHKATDYSIEWFKEELGILARSISDHFGTPVTT
                     DSLRRSIRLFNEIRSLLSELDEMRMSETCPVTGSDAASIVLAGFAMPREEYRETLGKV
                     MARLRKSKPVSDGKVRLMVVGSASDDADFVELVESTGAIVVSDCMCFGARSFLKRVDE
                     DADPIEALAERYLGGNLCPRMFGWYKDRLAFVRDRALRSRVDGVILQNIRFCDLHGSE
                     NGLLERDLEAEGIPCMRIEREYGPLVETGRIKMRLDAFMERIRTVGGRRS"
     gene            complement(1479..2684)
                     /locus_tag="PLT69_02920"
     CDS             complement(1479..2684)
                     /locus_tag="PLT69_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13428.1"
                     /translation="MGTQNGHDSPERILAGMIADAIRSAGTRRMGALVAEELARPWSA
                     KGALRRAAAWCVKKTSTLLAGADRTGSPRLSADLGRFVTAWAHKINEDHARRPDCHAA
                     ARKETIEGFIEHTDFGELREAFQGAAPCVLGTIGVFNEALWKYPAKVGSILGALLEIV
                     NTAVASLSELLAPIERKVGPDLLADLVLSLLRKLDAQALSALANRTFETIRRLHTGSL
                     LLSRSGRPLFQVYLTEKLEEFLSKTDPVLFVKAKKALDEDRVAVAGALADALSKHPGF
                     VTALVAASGAVATNTFKCASRKASLMDDLDREALSQAVSEALGGLDLFELGQALETVS
                     RVFNDLYDLRPDLVRSTMAALADSLAGPEFTRALGRVFAEALQAFSPLLEDLKSTSVG
                     MQAARGGAS"
     gene            complement(2662..3447)
                     /locus_tag="PLT69_02925"
     CDS             complement(2662..3447)
                     /locus_tag="PLT69_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13429.1"
                     /translation="MNMKRSRHTTRERRKDKRHDGKAAFAPVAALSPGDLMERKRQLV
                     KDTIIKVAEELFASQGYHNTQIIDIVKAVGMSAGTFYNYFRDKRDLFEQITKRNFETF
                     RAMIKKLREPIDIWDRSDRIAKLFETYTAYFDYIDTHRQQFLILLRGGFGVDEEFDGN
                     VWRHYSDIAKDLAEDIQSWIDMGIIAGLNPYTLAYAVVGMAMHLGHSYLMEQRFSREE
                     AISTLTSLSNNMFESYLTEAGRMALERPGTRDTGGADGDAERS"
     gene            complement(3458..4135)
                     /locus_tag="PLT69_02930"
     CDS             complement(3458..4135)
                     /locus_tag="PLT69_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13430.1"
                     /translation="MTRPRLDRRPKGALEALLSSTLFKDVLRTALNSADPARGAEAVN
                     TMLGRDPEAAFALVSSIPAAVNTLVSALTELALRLGNYPPALAGSMASAIARDVNKDV
                     VRACAAAWKGLARGVLASRPDLAAHLLVTVGVRVKAGALNALARFINDVHRHDPGVLS
                     AFFAEVLRRVDGDQARKACLALANAFLDQRWGIPSFAAELLATRARRSIERLLGKASG
                     KAMGHEG"
     gene            complement(4132..4584)
                     /locus_tag="PLT69_02935"
     CDS             complement(4132..4584)
                     /locus_tag="PLT69_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13431.1"
                     /translation="MDDTAITGFIETQAGLLHDFMSSPPAKDMLRLALKDIDPESGRR
                     LVRTLMTTDPEVPLAVAGSLPRAANLIIKALLELATLVRRSYPAPLLTGFVGSLLEEV
                     DRKDLALLRSEIAFFVREIGPVVKAWLESLDTLSGDDPAEGSEKGGRP"
     gene            complement(4594..5868)
                     /locus_tag="PLT69_02940"
     CDS             complement(4594..5868)
                     /locus_tag="PLT69_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13432.1"
                     /translation="MRHIDEFSRPEEYISPFDAHFRRLIRSHLEESVMPVRRRFDEDW
                     KEHRLIEPAFDRLMAGLGIQRALFPVEYGGWGLARSDYVFRMGYMLCEELGRADTGMA
                     VAVAVTFWPLLTILVEPYVNERLIAEFAPMFCNADHAVFAANAMTEPQGGSDIENLGL
                     LGGRTIRTTARLEGDRWVINGHKLWPTNSGGVAKLFGVPCTTKPGSSDPADFAFIFVP
                     ADTPGVTQGGPYEKAGMAADKNGDIWFEDVRVPSWYRAHGPGRDAEAFYQLIPFGQVM
                     SCAFLTGAMMNLYERLVEFVNTHTFAGRPLKEHDAVAAVIGRIAGDIDICRILGHEAA
                     MIGDRRNKPYGRPLASEQVVGRVRNIKDFISDTAIRCFETAMDVLGAYGADRQWDIEK
                     HWRDIKIVQLWMGGKQLCQMEAARYFFDCETL"
     gene            complement(5885..>6356)
                     /locus_tag="PLT69_02945"
     CDS             complement(5885..>6356)
                     /locus_tag="PLT69_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13433.1"
                     /translation="SWIHLLASGVCAGAAADFFDLLADWADSRTIKGGVPLKDNPLCA
                     SVLAESAQEIALARILVHDLADVVASAGAPEEGSDERAFVYACTIGRRILAGLIHALN
                     RGMELMGSAGYAKEWYVEKHWRDIKNVQSSLSGVGAGVPEAMDAARFFYGSSPR"
BASE COUNT         1011 a         2096 c         2083 g         1166 t
ORIGIN      
        1 ccgtgccgaa gatcgtgaac gcctccaggc agatggggac gaggtcgaag gcgtacagga
       61 tgggcgtggc catggagaac gaggtgagca ccgtcttctt gcccagggcc ttcgcctcga
      121 tgcacgcccg gatgtagttc gccacgagct tgatgaacct gaccccgggc tcgccctggc
      181 gtatgaagat gtccagggcg tcggcgaagt gggggatgag cccgagcagg ctctcgtact
      241 ccttgtccgt gccgtccttg acgagcgcgg cgttctccat gatggtccag atattccagt
      301 cgtaacggta ctccctggtt tccggcttca agaccgcctc ccccccaccg tgcggatacg
      361 ctccatgaag gcgtccaggc gcatctttat cctgccggtc tccacgaggg gtccgtactc
      421 cctctctatc ctcatgcacg ggattccctc ggcctcgagg tccctctcga ggagcccgtt
      481 ctctgagccg tgaaggtcgc agaacctgat gttctggagg atgaccccgt cgacccgcga
      541 tctcagggcg cggtctctca cgaaggcgag cctgtccttg taccacccga acatgcgggg
      601 gcagaggttg ccgcccaggt agcgctcggc aagggcctcg atcgggtcgg cgtcctcgtc
      661 cacgcgcttg aggaacgatc tcgccccgaa gcacatgcag tcgctcacca cgatcgcgcc
      721 cgtggactcg acgagctcca cgaagtccgc atcgtcgctc gcgctgccca cgaccatgag
      781 ccgcaccttg ccgtcggaga cgggcttgga cttcctcagc ctcgccatca ccttcccgag
      841 ggtttcccgg tattcctccc tcggcatggc gaagccggcc aggacgatgg atgcagcgtc
      901 cgaacccgtg acagggcacg tctcggacat cctcatctcg tcgagctcgg acaacagcga
      961 cctgatctcg ttgaagagcc ggatggacct cctgagggag tccgtcgtga caggggtgcc
     1021 gaagtggtcc gagatgctcc tcgcgaggat gccgagctct tccttgaacc actcgatgct
     1081 gtagtccgtc gccttgtgcg ggacccccat gtagtggaag aaaccgggca gggtccccgg
     1141 tatgtcctcg gatgcctttc tccaacactc gtgcagcctc ctcatggagt cgcacccgtt
     1201 caccacgacc gcgccgtcga ggaagccgta ggacccctgg cccgccagct ggaggatgca
     1261 cttgggaaag ctgcagatga ccggcccgaa gtaggcgtcg cctatggaca tggtgcctgc
     1321 ggtgatcccc ctgagccggt agggcaggat ccctgcggcg tcgatgatct cttcgggcac
     1381 gaaggtgcac gggtacccca ggaccttccc ccctccttca ttccaggcct ggagggaagg
     1441 gttgccgatg gagcgggcgg cctttctcag cgtctcgatc atgaggcacc tcctcgtgcc
     1501 gcctgcatgc ccacggaggt cgacttgagg tcctcgagca agggcgagaa ggcctggagt
     1561 gcctccgcga atacccggcc aagggccctg gtgaactccg ggcccgccag ggagtccgcg
     1621 agggcggcca tggtggaacg cacgagatcc ggcctgaggt cgtagaggtc gttgaagacc
     1681 ctggagacgg tctcgagggc ctgcccgagc tcgaagaggt caagcccgcc gagggcttcc
     1741 gagaccgcct gggagagggc ctccctgtcg agatcgtcca tgagcgaggc cttcctggac
     1801 gcgcacttga aggtgttcgt cgccacggcg ccggatgccg ccaccagtgc ggtcacgaac
     1861 cccgggtgct tggagagggc atcggccagg gcaccggcca ccgcgacccg gtcctcgtcg
     1921 agcgccttct tggccttgac gaacaggacg gggtccgtct tggagaggaa ctcttccagt
     1981 ttctccgtga ggtacacctg gaacaggggc ctgcctgacc tggacaaaag aaggctgccc
     2041 gtgtgcaggc ggcgtatggt ctcgaaggtg cggttggcga gcgcggacag cgcctgcgca
     2101 tcgagcttcc tgagaagcga caggacgagg tcggccagga ggtcgggccc gaccttcctc
     2161 tctatcggcg ccaggagctc ggacagggag gcaacggcgg tgttcacgat ctcgagcagg
     2221 gcgcccagga tgctgccgac cttggcgggg tacttccaca gggcctcgtt gaacaccccg
     2281 atcgtcccga ggacacaggg cgcggcgccc tggaaggcct ccctgagctc gccgaagtcg
     2341 gtgtgctcga tgaagccctc tatggtctcc ttgcgcgcgg ccgcgtggca gtcgggcctt
     2401 cgcgcgtggt cttcgttgat cttgtgcgcc cacgcggtca cgaacctgcc caggtccgcc
     2461 gagagccttg gggatcccgt cctgtccgca ccggcgagca gggtggaggt cttcttcacg
     2521 caccaggccg cggccctcct caatgcccct ttcgcggacc atggccgcgc gagttcctcc
     2581 gcgaccagtg cccccatgcg cctggtcccc gccgagcgga tggcatcggc gatcatgccg
     2641 gcgaggatcc gctccggcga atcatgaccg ttctgcgtcc ccatctgcac ctcccgtgtc
     2701 tctggttccc ggtctctcga gcgccatcct ccccgcctcg gtgaggtagg actcgaacat
     2761 gttgttgctg agcgaggtca gggtcgatat cgcctcttcc ctcgagaacc tctgttccat
     2821 gaggtagctg tggcccaggt gcatggccat cccgaccacg gcataggcca gcgtgtacgg
     2881 gttcaggccg gcgatgatgc ccatgtcgat ccacgactgg atatcctcgg ccaggtcctt
     2941 cgctatgtcg ctgtagtgcc gccagacgtt cccgtcgaac tcctcgtcga cgccgaaccc
     3001 cccgcgcagg aggatgagga actgctggcg gtgcgtgtcg atgtagtcga aatacgcggt
     3061 gtaggtctcg aagagcttcg cgatcctgtc ggagcggtcc catatgtcga tgggctccct
     3121 gagcttcttg atcatggccc tgaacgtctc gaagttccgc ttggtgatct gctcgaacag
     3181 gtcgcgcttg tccctgaagt agttgtagaa ggtgcctgcc gacatgccga cggccttcac
     3241 gatgtcgatg atctgggtgt tgtggtagcc ctgggaggcg aagagctctt ccgccacctt
     3301 tatgatcgtg tccttgacga gctgccgctt tctttccatc aggtccccgg gcgacagggc
     3361 ggcgaccggg gcgaaggcgg ccttgccgtc atggcgtttg tccttccgcc tttccctggt
     3421 ggtgtgcctc gatctcttca tgttcatggg cgcagcctca tccctcatgg cccatggcct
     3481 ttccggacgc tttcccgagg agccgctcga tcgacctcct tgccctggtg gcgagcagct
     3541 ccgccgcgaa ggaggggatg ccccagcgct ggtccaggaa ggcgttcgca agcgccaggc
     3601 atgccttcct cgcctggtcg ccgtcgaccc tccgcaggac ctcggcgaag aacgcgctca
     3661 agacgccggg atcatgcctg tgcacgtcgt ttatgaacct cgccagcgca ttgagggccc
     3721 ccgccttgac ccggacgccc acggtcacca ggaggtgtgc ggcaaggtcg ggacgggagg
     3781 ccaagacacc ccttgcgagc cccttccagg ctgccgcgca ggcccgcacg acatccttgt
     3841 tgacgtcccg ggcgatggcc gaggccatcg agcctgcgag ggcgggcggg tagttcccga
     3901 gcctgagagc gagctccgtg agggcgctca cgagggtgtt cacggccgcg gggatgcttg
     3961 agacgagggc gaaggccgcc tccgggtctc tcccgagcat cgtgttcacg gcctcggcgc
     4021 cccgtgcggg gtccgccgag ttcagggcgg tcctgaggac gtccttgaag agggtggagg
     4081 aaaggagggc ctccagggcg cctttcggtc gtctgtcgag gcggggtcgc gtcatggcct
     4141 gccccctttc tcggagcctt cggcggggtc gtctccgctt aaggtgtcca aggattcgag
     4201 ccacgccttg accacggggc cgatctcgcg cacgaagaag gcgatctccg acctcagcag
     4261 ggcaaggtct ttcctgtcca cctcctcgag gagcgacccc acgaaacccg taagaagcgg
     4321 ggcggggtag gagcgcctca cgagggtcgc gagctcgagg agggccttga tgatgaggtt
     4381 cgcggccctg ggcaaagagc ccgcgacggc gagcgggacc tcggggtcgg tggtcatgag
     4441 cgtgcgtacg aggcgtcttc ccgattccgg gtcgatgtcc ttgagcgcaa gcctcagcat
     4501 gtccttggcc ggtgggctcg acatgaaatc gtggagcagg cccgcctgcg tctcgatgaa
     4561 accggtgatc gcggtatcgt ccatggctac acctcacaag gtctcgcagt cgaagaagta
     4621 cctcgccgcc tccatctggc agagctgctt gcctcccatc cacagctgga cgatcttgat
     4681 gtccctccag tgcttctcga tgtcccactg cctgtcagcg ccataggcgc ccagcacgtc
     4741 catggcggtc tcgaaacacc tgatcgcggt gtccgatatg aagtccttga tgttgcgcac
     4801 ccggccgacc acctgctcgc tggcgagcgg cctgccgtag ggcttgttcc gcctgtcccc
     4861 gatcatggcg gcctcgtggc cgaggattcg gcaaatgtcg atgtcgccgg ctatgcggcc
     4921 gatcacggcg gcgaccgcgt cgtgttcctt gaggggcctg cccgcgaagg tgtgcgtgtt
     4981 cacgaactcg acgagcctct cgtagaggtt catcatggcg cccgtgagga aggcgcagct
     5041 catgacctgc ccgaagggga tgagctggta gaacgcctcg gcgtccctgc cggggccgtg
     5101 ggcccggtac caggacggga cgcgcacgtc ctcgaaccag atgtccccgt tcttgtcggc
     5161 cgccatgccc gccttctcgt acggcccgcc ctgggtgacg cccggcgtgt cagcgggcac
     5221 gaagatgaag gcgaagtctg cggggtccga ggacccgggt ttcgtggtgc aggggacgcc
     5281 gaagagcttg gccacgccgc ccgagttcgt gggccagagc ttgtggccgt tgatcaccca
     5341 ccggtcgccc tcgagcctcg ccgtggtgcg gatggtcctc ccgccgagca gacccaggtt
     5401 ctctatgtcc gagccgccct ggggctcggt catggcgttc gccgcgaaca cggcgtggtc
     5461 ggcgttgcag aacatgggcg cgaactcggc gatcaggcgc tcgttcacgt acggttccac
     5521 caggatggtc aagagcggcc agaaggtcac cgccaccgcc accgccatgc cggtgtcggc
     5581 cctgccgagt tcctcgcaga gcatgtaccc catcctgaac acgtagtcgg agcgcgcgag
     5641 gccccacccg ccgtactcga cggggaacag ggccctctgg atgccgaggc cggccatgag
     5701 acggtcgaag gcgggttcga tgaggcggtg ctccttccag tcctcgtcga agcgcctcct
     5761 cacgggcatg accgactcct cgaggtgcga tcgtatgaga cgccggaaat gggcgtcgaa
     5821 cggggagatg tattcctcgg gccgcgaaaa ctcgtctatg tgtctcatcc tcgatcctcc
     5881 ctcctcacct cggcgagctg ccgtagaaga acctggccgc gtccatggcc tcgggcaccc
     5941 ccgcacccac acccgagagg ctcgactgga cgttctttat gtcccgccaa tgcttctcga
     6001 cgtaccactc ctttgcgtaa ccggcggagc ccatgagttc catgccccgg ttcagggcgt
     6061 gtatgaggcc ggccaggatg cgtctcccga tggtgcacgc gtacacgaag gccctctcgt
     6121 ctgacccttc ttccggtgca cccgcggacg cgaccacgtc ggcgagatcg tgcacgagga
     6181 tgcgggccag cgcgatctcc tgggccgact cggccaggac cgaggcgcac agcgggttgt
     6241 ccttgagggg cacgccgccc ttgatggttc gcgagtccgc ccagtcggcg agcaggtcga
     6301 agaagtcggc ggcggcgccg gcgcacaccc cggaggcgag gaggtggatc caggag
//
LOCUS       DAOSCR010000069         6344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7826_length_6344_cov_3.612657, whole genome shotgun sequence.
ACCESSION   DAOSCR010000069 DAOSCR010000000
VERSION     DAOSCR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7826_length_6344_cov_3.612657"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1091
                     /locus_tag="PLT69_02950"
     CDS             192..1091
                     /locus_tag="PLT69_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323938.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HOJ13434.1"
                     /translation="MEYFLELFFGGLTRGSIYALIALGYTMVYGIIELINFAHGEIYM
                     IGAFTALIVASALSLSGMSGWSLLALAGAVAVVYSAAYGYTLEKIAYKPLRNAPRLSP
                     LISAIGMSIFLQNYVLLAQTSDFVSFPALIEEFPFMEDVTFMRSSELVIFVVTVVATA
                     LLTLFIKFTKTGKAMRAVAQDKTMAMLLGVNVNRVISVTFIIGSALAAIGGVLISSHI
                     GQINFYIGFIAGIKAFTAAVLGGIGSIPGAVLGGMVLGWTESFATGYVSSDYEDVFAF
                     GLLVFILIFRPAGILGRTTSEKV"
     gene            1182..2359
                     /locus_tag="PLT69_02955"
                     /pseudo
     CDS             1182..2359
                     /locus_tag="PLT69_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            2371..3165
                     /locus_tag="PLT69_02960"
     CDS             2371..3165
                     /locus_tag="PLT69_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13435.1"
                     /translation="MTKTPVLEVKGLTMDFGGLRALENIDLSVHEGEIVAVIGPNGAG
                     KTTFFNCLTGIYEPTAGDMVANPPGKPSRRLNRLKPNVVTSIGLARTFQNIRLFQKMT
                     VLENVMVARHVRTSSGVFGAVFRDARTRREEKETVEFSYRMLERVGLKDYVNEIASEL
                     PYGAQRRLEIARALATEPFLLLLDEPAAGMNPKETSELDELIKSIRDELRVSIVLIEH
                     DMKLVMSISDRIYVLDYGQLIAHGTPREVASDPRVIKAYLGEAFDA"
     gene            3158..3865
                     /locus_tag="PLT69_02965"
     CDS             3158..3865
                     /locus_tag="PLT69_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13436.1"
                     /translation="MLKVKNIHTYYGGIHALKSVSLELRQGEIVTLIGANGAGKSTTL
                     MSISGVVPPRYGEIIFMGEPIQGLSPDAIFGRGIVQVPEGRRIFPRLTVVENLEMGAY
                     LRKDKRGIRQDMDRIFTLFPRLAERRHQLGGTLSGGEQQMLAISRGLMASPKLLLLDE
                     PSLGLAPVLVQLIFETIERINRENKTTIFLVEQNANMALRIAHRGYVMESGAIAMEDT
                     AANLLKSEKVKEAYLGL"
     gene            complement(3840..4838)
                     /locus_tag="PLT69_02970"
     CDS             complement(3840..4838)
                     /locus_tag="PLT69_02970"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ13437.1"
                     /translation="MHYSTCMAKRIPVMSWVTLILFGLCVCVLLFADFGKGGSLVVDS
                     LLDFGHVPLFVLVTAVVLRVLEPGGWLKADVQTYVRAFALSVFFGVSSEIVQHFMPGR
                     SFQLGDIVRDAAGALAFVLLAYRHKRPMRPQRRAAIAGIACLAVLGAFAPVVSAASDE
                     LRARRDFPLLSSFETAFEMKRWVIKEGSSQRVRMHATHGLYSLEVRLEPGTYPGVTLD
                     FPPRDWRGFDALAFDVHVGGPSPLPLTVRINDLEHNEEYTDRYNKTFVLGPGANHVVI
                     DLAEVKRAPRGRAMDMGRMALVCLFSYRLKEPRTIYLDNVRLQAGLDASTARGTPP"
     gene            5204..6049
                     /locus_tag="PLT69_02975"
     CDS             5204..6049
                     /locus_tag="PLT69_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13438.1"
                     /translation="MHNDAFDDLAKYPKDAPSRKLHVLFIHHSTGGQLLADPGPDDGR
                     NCIYKTHPNGGGLRRMLEENNYVVHEASYGSIIGNETDICHWNAKFRDQMDKILSCNN
                     QDESFTDGTKNSVVIFKSCFPNSWIDSEGSGPGDPDSCDRTTANYRAAYKALLTYFAA
                     RPDTLFVAFTAPPLAMPLKAKAKNIIKSLLGRSDDYRTVGRRNRAFNAWLTDTKKGWL
                     EAYPHKNVVVFDYYDVLTGHGASDWSVYGSQGGTDSHPSSEGNSMAARQFVPFLNRAV
                     NRMGM"
     gene            6165..>6344
                     /locus_tag="PLT69_02980"
     CDS             6165..>6344
                     /locus_tag="PLT69_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13439.1"
                     /translation="MTLLDLFRKAFQAVTLGDVTLPGLWHLAKGLARGTFYILWYRST
                     RRNVVIRFPFMAYAPV"
BASE COUNT         1191 a         2002 c         1995 g         1156 t
ORIGIN      
        1 ggggtcaagc cgaagggagc aggcttcctg ctccctttct ttataccctt ttcctgcggg
       61 agaccacggc cggccggaaa gacacgggag acaggcggat gcaacagacg gcgacacgcg
      121 gcgatccggt cataagcggt gtgaaccggc ttcatgtcgg gcacgagggc gctgccaggg
      181 ggtgagccgc tatggaatac ttccttgagc tcttcttcgg gggactcacc cggggcagca
      241 tatacgccct catcgccctc ggctacacca tggtctacgg catcatcgag ctcatcaact
      301 tcgcccacgg cgagatctac atgatcggcg cgttcaccgc gctgatcgtg gcgagcgcgc
      361 tgtcgctctc cggcatgagc ggctggtcgc tgctcgcgct cgcgggcgcc gtggcggtgg
      421 tctactcggc ggcatacggg tacaccctcg agaagatagc gtacaagccc ctgaggaacg
      481 cgccgcggct ctcgccgctc atcagcgcca tcggcatgtc catcttcctc cagaactacg
      541 tgctcctcgc ccagacgtcg gacttcgtct ccttcccggc tctcatcgag gagttcccct
      601 tcatggagga cgtgaccttc atgcggtcgt cagaactcgt gatcttcgtc gtcaccgtgg
      661 tggccacggc cttgctgacg ctcttcatca agttcaccaa gaccggcaag gccatgcggg
      721 cggtcgccca ggacaagacc atggccatgc tgctcggcgt gaacgtgaac agggtcatct
      781 cggtgacctt catcatcggc agcgcgctcg ccgccatagg cggggtgctc atctcgtctc
      841 acatcggcca gatcaacttc tacataggct tcatcgccgg catcaaggcc ttcaccgcgg
      901 ccgtgctcgg agggatcggc agcatcccgg gcgccgtgct gggaggcatg gtgctcggct
      961 ggaccgagag cttcgccacc ggctacgtct cgagcgacta cgaggacgtg ttcgccttcg
     1021 gcctgctcgt gttcatcctc atcttcaggc cggcgggcat cctcggcagg acgacttccg
     1081 agaaggtgtg aggagggcgt gggggaggta tggacctgca tgtcttcgcg agacgcgcgt
     1141 tgagggaagc ggccctttcc gcggggaccg ccctgtggtt catgttcctc acgttcccca
     1201 tcatggtgat gaagatctcg cccttggaca agaccatcca gtggcgcttg aacaggatgg
     1261 tctgggtggg ggtgagcacc ttcgtcctgt ccctcgtgtg gcgctacctc atggcgagga
     1321 gagaccgggg gggctcccgc gaggaggggc ggagctggca gaagaggatc ggcgagcgca
     1381 tccggtctga caggaggctc tccgtggggc tgctcgcggc cctcggcgtc cttgtcctgt
     1441 ccttcccgat ggtcttctcc acctaccaga cctcgatcat gataacggcc ctcatgtacg
     1501 tgatgctggg gctcggcctc aacatcgtgg tgggccttgc cgggctgctg gatctcggct
     1561 acgtggcgtt ctacgccgtg ggggcctatt tctacgcgct catgaaccac cacttcggcc
     1621 tgggtttctg ggtggccctg ccgttcgcgg gccttctagc cgcgaccttc ggcgtgctcc
     1681 tgggcttccc ggtgctcagg ctcaggggag actacctggc catcgtcacg ctcggcttcg
     1741 gcgagatcat ccgcctcatc ctcgagaact ggaacgagtt ttccttcggc ccgagcggca
     1801 tagccgggat ctcgcggccg ggatccttcg ggatggagct cacgctcgac cagcacatcg
     1861 tctacctcta ctacatcatg gtggccctcg tctgcttcac cgtcttcgtg gtgggccggc
     1921 tccgggattc gcgcattggc agggcctgga tagccctcag ggaggacgag atcgcctgcc
     1981 aggccatggg gatcgacaag acgaagacga agctcatggc ctttgcgctc ggggccacgt
     2041 ggcagggttc gtcgggtgta tcctggctgc caagaccacg ttcatcaacc ccgcgagctt
     2101 caccttcatg gagtcggcca tcatcctgtc catcgtggtc ctggggagca tgggctccat
     2161 cgtgggcgtc atcgtgggcg cgttcttcct catcctgctg ccggaatacc tgagggcgtt
     2221 ctccgagtac cgcatgatcg ccttcggcgc cgccatggtg ctcgtcatgg tgttcaggcc
     2281 gcaggggctc atcagcgcgc tcaagaggca ctacgagttc agggccggcc ccgccggcaa
     2341 ggagggcgcg ccgtcgtgac gcaggcgggg atgacaaaaa cgccggtgct cgaggtcaag
     2401 ggcctcacaa tggatttcgg gggcctgcgt gccctcgaga acatcgacct gagcgtgcac
     2461 gagggggaga tcgtggccgt catcgggccc aacggcgccg ggaagacgac cttcttcaac
     2521 tgcctcacgg ggatctacga gccgaccgcg ggagacatgg tcgcgaaccc gccggggaag
     2581 ccatcgaggc gcctgaaccg cctgaagcct aacgtcgtca cctccatagg ccttgcgcgg
     2641 accttccaga acatcaggct cttccagaag atgaccgtgc tcgagaacgt gatggtcgcg
     2701 cgacatgtcc gcacgagctc aggcgtcttc ggcgccgtct tcagggacgc gcgcacgagg
     2761 agggaggaga aggagacggt ggagttctcc taccggatgc tcgagcgggt tgggctgaaa
     2821 gactacgtga acgagatcgc gtccgagctg ccctacggcg cgcagcgcag gctcgagata
     2881 gcccgcgcgc tcgccaccga gccgtttctg ctcctgctcg acgagcctgc cgcggggatg
     2941 aacccgaagg agacctccga gctcgacgag ctcatcaaga gcatccgcga cgagttgagg
     3001 gtctcgatcg tgctcataga acacgacatg aagctcgtca tgagcatatc ggacaggatc
     3061 tacgtgctgg actacggcca gctgatcgcg cacgggacac ccagggaggt ggcctccgac
     3121 cccagggtca tcaaggccta cctcggggag gccttcgatg cttaaggtga agaacatcca
     3181 cacctactac gggggcatcc acgccctcaa gagcgtgagc ctcgagctca ggcagggcga
     3241 gatcgtgacg ctcatcggcg ccaacggcgc gggcaagtcg accaccctca tgtccatatc
     3301 cggcgtggtg ccgccccgct acggcgagat catcttcatg ggtgagccca tccagggact
     3361 gagccctgac gccatcttcg gcaggggcat cgtccaggtg ccggagggca ggcgcatctt
     3421 cccccggctc accgtggtgg agaacctgga gatgggcgcc tacctcagga aggacaagag
     3481 gggcatcagg caggacatgg atcgcatctt cacgctgttt ccccgcctgg ccgagaggag
     3541 gcaccagctt ggcggcaccc tgtctggggg tgagcagcag atgctcgcca tctcgcgcgg
     3601 tctcatggca agcccgaagc tgctcctgct cgacgagccg tcgctcgggc tggccccggt
     3661 gctcgtccag ctcatcttcg agaccatcga gcgcatcaac cgggagaaca agacgaccat
     3721 cttcctcgtg gagcagaacg ccaacatggc gctgcgcata gcccacaggg gctacgtcat
     3781 ggagagcggc gccatcgcca tggaagacac ggcggccaac ctcctgaaga gcgagaaggt
     3841 caaggaggcg tacctcgggc tgtagacgcg tcgagcccgg cctgaagcct cacgttgtcg
     3901 aggtagatgg tcctcggttc cttcagacgg taagaaaaca ggcacacgag cgccatgcgg
     3961 cccatgtcca tggccctgcc ccttggggcg cgcttcacct cggccaggtc gatgacgaca
     4021 tggttcgcgc caggccccag cacgaaggtc ttgttgtacc ggtccgtgta ctcctcgttg
     4081 tgttcgaggt cgttgatgcg cacggtaagc ggcagcgggg aagggccgcc cacatgcacg
     4141 tcgaatgcga gcgcgtcgaa cccccgccag tcgcgcgggg ggaagtcgag cgtgacccca
     4201 gggtatgtcc ccggctccag ccgcacctcg agggagtaca ggccgtgcgt cgcgtgcatg
     4261 cgcaccctct gcgagcttcc ttccttgatc acccacctct tcatctcgaa agccgtctcg
     4321 aaggaggaga gcagcgggaa gtccctcctc gcccggagct cgtccgaggc tgccgagacc
     4381 acaggggcga acgccccgag caccgcaagg catgcaatcc cggcgatcgc cgcgcggcgt
     4441 tgcggccgca tgggcctctt gtgccggtac gcaaggagca cgaaggcgag ggcgcccgcg
     4501 gcgtccctca cgatatcacc cagctggaac gaccgcccgg gcatgaagtg ctggacgatc
     4561 tcgcttgaga ccccgaagaa gacgctcagc gcgaatgccc gcacgtatgt ctgcacatcg
     4621 gccttcagcc agccccccgg ctcgagcacc cgcaggacaa cggccgtgac cagcacgaag
     4681 agcggcacgt ggccgaaatc gagcaggctg tccaccacca gggagccccc tttgccgaaa
     4741 tccgcaaaga ggagcacgca tacgcacagg ccaaagagga tcagcgtcac ccatgacatg
     4801 acagggatgc gtttcgccat gcatgtgctg tagtgcacgt tgacggcgtt gcgcaagtga
     4861 gatagtatgc aagcgaattg aaatacggtg gaatcaaggc aagtcttctc cgtaaggccg
     4921 cgtataccat aaagtttgcc agagagcatg acgagtaggt aaagaggcct caatgcctca
     4981 tctctcgtgc ttcactcgca attgtgatac atatcatgcg ggtttcctct tcacccgctt
     5041 gagcggagct atggaaaaga tttcccgagg tatcgccgtt gaccgttcgg cttttcgttt
     5101 cgcgccttgc atggcagcgg ccgttgtcct ctggctgctt gtatcgtgca gcgaccaagg
     5161 cagcgtgaca ccaccacggg atgatgcaaa gcaggaggcc aagatgcaca acgatgcctt
     5221 cgacgacctg gccaagtacc cgaaagacgc cccatcgaga aagctgcatg tcctcttcat
     5281 ccaccattcg acaggcgggc agctccttgc ggaccctggg cccgatgacg gcaggaactg
     5341 catctacaag acccatccca acggcggggg cctgaggcgc atgctcgagg agaacaacta
     5401 cgtggtgcac gaggcgtctt acggttccat catcgggaac gagacggaca tctgccattg
     5461 gaacgcaaag ttcagggacc agatggacaa gatcctatcc tgtaataacc aagacgagtc
     5521 tttcacggac gggacgaaaa acagcgtcgt gatcttcaag tcatgctttc cgaacagctg
     5581 gatagactct gaaggctccg gccccggcga cccggactcc tgcgatcgca cgacagcgaa
     5641 ctacagggcg gcctacaagg cccttctaac atacttcgcc gccaggccgg acacgctctt
     5701 cgtggccttc actgcgccgc cgctcgccat gcccctgaag gcgaaggcga agaacatcat
     5761 caagagcctt ctcggaaggt cagatgacta tcgcacggtg gggaggcgga acagggcgtt
     5821 caacgcatgg cttacagaca cgaagaaagg ctggctcgag gcctacccgc acaagaacgt
     5881 cgtcgtcttc gactactacg acgtgctcac ggggcacggc gcatccgact ggtctgtcta
     5941 cgggtcgcaa ggcgggacgg acagccatcc gtcgtccgag ggaaactcga tggctgcaag
     6001 gcagttcgtg cccttcctga accgggccgt caaccgtatg ggcatgtgat gcaaggcttc
     6061 tccttgtctg cgggtgcatg tggacaaccc tggaaggcac ccttggtggt gcgggcctgc
     6121 acgaccgatc cccaggtgca cgggatgacc gtgggtgttg aaggatgacg ctcctcgatc
     6181 ttttccgcaa ggcgttccag gccgtcacgc taggggatgt cacgctgccg ggcctttggc
     6241 acctggcaaa aggcttggcg cgcggtacgt tctacatcct ctggtaccgc tcgacgaggc
     6301 ggaatgtcgt catcaggttc cctttcatgg cgtacgcccc tgtg
//
LOCUS       DAOSCR010000070         6326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_7852_length_6326_cov_4.025195, whole genome shotgun sequence.
ACCESSION   DAOSCR010000070 DAOSCR010000000
VERSION     DAOSCR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_6326_cov_4.025195"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1447
                     /locus_tag="PLT69_02985"
     CDS             368..1447
                     /locus_tag="PLT69_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ13440.1"
                     /translation="MNDIFERLRERLDSMATGYPKTASGVETRILRRLFGEEDARVFL
                     AMGDAPEDPVSLAGRMRLEPRDMAVRLEDMARRGLVFRMKRGGQTLYLAVPFVVGIYE
                     FQLNNLDPELLGDISEYYLSGLGASFHSLRTPHLRTIPIDTRIASGGPVAPYDDARAI
                     VGSKTKIALAECFCRKAVRVYGKTCSHPMETCMQFDAFAEYYLDNRMARPVSKDEAMA
                     VLDMCEEEGLVIHPVNSKSVEAMCACCSCCCGMLLSLKLFAAPALQAKSTYTCAFDPS
                     LCTSCGACGSRCPVGALRFKDGAVRLRPERCIGCGLCVSRCPAGALTLKKKDRGIEPP
                     PETVFDTFALMRGERVDGNEGQEGE"
     gene            1745..1945
                     /locus_tag="PLT69_02990"
     CDS             1745..1945
                     /locus_tag="PLT69_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOJ13441.1"
                     /translation="MAQGTVKWFNREKGYGFITADGGQDVFVHHTSINQQGFRSLEEG
                     QRVTFDIVKGPKGMQASNVTVA"
     gene            2395..4800
                     /locus_tag="PLT69_02995"
     CDS             2395..4800
                     /locus_tag="PLT69_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOJ13442.1"
                     /translation="MVRKINKAAVIGSGIMGGGIAALLAAAGVDMLLLDIVPFDLKEE
                     EKKDKKARNRIVTMGLENALKAKPPLFYNKDHAMRITTGNLEDDFDKLAECDWIIEVV
                     VENLKIKQDLFARIDKVRKPDAIVSSNTSGIPLKAMSEGRSKGFKEHFLGTHFFNPVR
                     YMHLLEIIPGEETKKEVLDFIAEFGESRLGKGIVWAKDTPNFIGNRIGIHGIGATLKA
                     MLEEGLTIPEVDAIFGAPMGRPKTAIFATGDLVGLDTMTHVMKNTYDLCPNDEEREVF
                     KVPEFLQKMLDQKLLGNKTKAGFYKKEVTPDWKKVKKVIDPKTGEYVVAEKASFPCLD
                     EAKKAKTLPEKLKAVLYGDDKGAKFAWKVQASAFIYAANRIPEISDTVVEIDNAMKWG
                     YNFEMGPFETWDAIGVKESVEKMEKDGFKVPEKVKKMLAKGATSFYKLENGKKMFWDF
                     ASETYKPVKTSPKALQLSNFKAENKTVLTSGSCSLIDIGDGVFCLEFHSKMNAINKEM
                     VEFMAKAGEYVGANGVGMVIGNQAGGMPGAFSAGGDLAYMLGLARAGKFSEIDAFIRE
                     VHKGIMATRYSNYPVVAAPYGLTLGGGCEVCLSADRIVAHADLFMGLVEIGAGLVPGG
                     CGMINLWRKFITSKPEDAKVTDYGGFFLNAFMAVAQAKVSNSAEQARQRGFLGAADRI
                     VMNRDNLIGEAKKEVLKMVDDGYAPPIKKKIPVFGREAHGMVWAEMNNMKGGGYITPH
                     MEFIAKKICICMSGGDVPCGAEVTEEHLMTLEREAFVELWKTENTQKMAEHIMNTGKP
                     LFL"
     gene            4835..6052
                     /locus_tag="PLT69_03000"
     CDS             4835..6052
                     /locus_tag="PLT69_03000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOJ13443.1"
                     /translation="MREAYIVQSVRTPGCRRARGAFAQTRPEDLLKAALNGLMERTPG
                     VEKKDVEDILVGCAFPEAEQGLNIGRIIAQMCKFPDDTCGATVNRFCASGLEAISLQA
                     MRIMAGMCDVAIGGGLESMSIVPMGGNMPRPHPDWAKENPDVYISMGMTAENVANRYK
                     ITREMQDRFGYSSQMKAAKAQKEGKFKEIVPVEAVRFKEKDGILVKETFIQDFDDGVR
                     ADTTIEGLSKLRPAFAAMGSVTAGNSSQTTDGAAATLLMSGEAVKKFGVKPIAKFKAY
                     VVAGCRADEMGVGPAYAIPKLLKVTGLGTAADIAKDAIFEINEAFASQAIYCLQQIGL
                     YDDKYHWFESDERIVNVNGGAIALGHPLGCTGAKLCAQLMTEMQARKKKWGIESMCIG
                     GGMGAAALFELCD"
BASE COUNT         1347 a         1909 c         2000 g         1070 t
ORIGIN      
        1 gtttcagctc atcggcctca cacccggctc gtacgagatc acccccaccg acccttcatg
       61 ccggggatcg gcggtcatcg tgatacccga cggggtgcag ggggtgttcg atgcggggac
      121 cgtgaccttg cccggcccct cctgattcct cgaggcacaa gacaggtggt gagctctttc
      181 cgctaagcgg cctccacagg gcgcgtcctg cctatcggcg ggcccgccat ggcgcgtcgt
      241 acccgggggc ctggaggtct ttccttgatt catggacacc tgcctgctag acatcgacgg
      301 aggtgggaac cgccggacgg gaccgagggg cgacgggaag ggccggagaa agggggccgg
      361 aaaggacatg aacgacatat tcgaaaggtt gcgcgagagg ctcgactcca tggccacggg
      421 ctacccgaag accgcaagcg gggtggagac gaggatcctg aggaggctct tcggcgagga
      481 ggatgccagg gtcttcctcg ccatgggaga cgcacccgag gatcccgttt ccctggccgg
      541 gaggatgcgc ctcgaacccc gggacatggc cgtgcggctg gaagacatgg cgagaagggg
      601 gctcgtgttc aggatgaaga gaggcgggca gacgctctac ctggcggtac ccttcgtggt
      661 cgggatctac gagttccagc tgaacaacct cgatccggag ctcttgggtg acatctccga
      721 gtactacctc tcggggctcg gcgcgtcctt ccattctctc cgaacgcccc acctcagaac
      781 gatccccatc gatacgcgga tcgcatccgg cgggccggtg gccccctacg acgacgccag
      841 ggcgatcgtc ggttcgaaga cgaagatcgc cctcgcagag tgcttctgcc gcaaggcggt
      901 gagggtgtac ggcaagacgt gttcccaccc catggagacg tgcatgcagt tcgacgcgtt
      961 cgcagagtac tacctcgaca accgcatggc gaggcctgtc agcaaagacg aggccatggc
     1021 cgtcctcgac atgtgcgagg aagaggggct cgtgatacac cccgtcaatt ccaagagcgt
     1081 cgaggccatg tgcgcctgct gctcgtgctg ctgcggcatg ctcctctccc tgaagctctt
     1141 tgccgcccct gcccttcagg ccaagagcac ctacacgtgc gccttcgatc cttccctgtg
     1201 cacctcgtgc ggcgcgtgcg gatcacggtg tcccgtgggt gcgctcaggt tcaaggacgg
     1261 cgcggtaagg ctgagacccg agaggtgcat cgggtgcgga ttgtgcgtgt cgcggtgtcc
     1321 cgcgggggcc ctcaccctga agaagaagga ccggggcatt gaaccgccgc ccgagaccgt
     1381 gttcgacacc ttcgcgctga tgaggggcga gagggtcgac ggaaacgaag ggcaagaagg
     1441 cgagtagatc ccggcgacta tgtcgcaggc agcttcatcg gcggcacctt gtgaaagagg
     1501 ggaaacgttc ttgcctcgag ggccgcgttg ccccgtgcat tttccccttg acaccgaagc
     1561 gtccgtacca tacaagggac tccatctgaa aagatgaaaa aacatatctg agttgaacag
     1621 tgacctgaag cccgccttca gtatccgctc cacacagtcg actcttcatc gaggatatct
     1681 tcacttttca gaaattcaac aaatgcaccg cccaaaaagg caagcggtgc gagagaggtc
     1741 attcatggca caaggaacag tcaagtggtt caacagggag aagggttacg ggttcatcac
     1801 tgctgacggc gggcaggacg ttttcgttca ccacacctcg atcaaccagc agggtttccg
     1861 gtcgctggaa gaaggtcagc gcgtgacctt cgacatcgtc aagggcccca aggggatgca
     1921 ggcgtcgaac gtcacggtgg cttaatcggc cgcgttcttg cgtaaccaca aacactcgag
     1981 gcggggtgga ctgttcgtcc gccccgcctt ttctcttctt ggaggtttgg tatcaaggcg
     2041 aactcgtgtc caggtgccgt atccctttcc tcgggcccgt catcggtttt ggggcgagga
     2101 aggggcaaag cccctcgtgc ggctcgaacg atctcgtctt cagcgatggc ccggcgtgag
     2161 acggcaggtg cttgcgggtc ttccttgcgg gcggcccttc gcgggtatgt cgcgttcatg
     2221 tcgtaaaagg gcatgagcgc gcgttcgtga ggacatggaa ggaaggggag aaaatctttt
     2281 cctggcgaga cgagagcggt tgacatggcc ccccttcggg tgtacaatga atgttgattc
     2341 agagttcact gtttcaaaag gcacggtcaa ccaactaaaa cagaggaggg tataatggtc
     2401 aggaaaatca ataaggccgc ggtcatcggg tctggaatca tgggaggcgg cattgcagcg
     2461 ctcctcgccg cagccggcgt ggacatgctc ctgctcgaca tcgttccgtt cgacctgaag
     2521 gaagaggaga agaaagacaa gaaggccagg aaccggatcg tcaccatggg cctggaaaac
     2581 gcgctgaagg ccaagccgcc gctcttctac aacaaggacc acgccatgcg catcaccacc
     2641 ggcaacctcg aggacgactt cgacaagctg gccgagtgcg actggatcat cgaggtggtg
     2701 gtcgagaacc tcaagatcaa gcaggacctg ttcgcgcgca tcgacaaggt ccgcaagccc
     2761 gacgccatcg tatcctccaa cacctcgggt atcccgctca aggcgatgag cgagggccgg
     2821 agcaagggct tcaaggagca cttcctcggc acgcacttct tcaacccggt ccgctacatg
     2881 cacctgctcg agatcatccc gggcgaggag accaagaagg aagtcctcga cttcatagct
     2941 gagttcggcg agagcaggct cggcaaggga atcgtctggg ccaaggacac cccgaacttc
     3001 atcggcaacc gcatcggcat ccacggcatc ggcgcgacgc tcaaggccat gctcgaggaa
     3061 gggctcacca tccccgaggt ggacgccatc ttcggagcgc ccatgggcag gcccaagacc
     3121 gctatcttcg ccaccggcga cctcgtcggc ctcgacacca tgacccacgt catgaagaac
     3181 acctacgacc tctgccccaa cgatgaggag cgcgaggtgt tcaaggtccc cgagttcctc
     3241 cagaagatgc tcgaccagaa gctgctcggc aacaagacca aggccggctt ctacaagaag
     3301 gaggtcacgc ccgactggaa gaaggtcaag aaggtcatcg accccaagac cggcgagtac
     3361 gtggtcgccg agaaggccag cttcccctgc ctggacgagg ccaagaaggc caagaccctg
     3421 cccgagaagc tcaaggcggt cctctacggt gacgacaagg gcgccaagtt cgcatggaag
     3481 gtccaggcgt ccgcgttcat ctacgcggcc aaccgcatcc ccgagatctc tgacacggtc
     3541 gtggagatcg acaacgccat gaagtggggc tacaacttcg agatgggccc cttcgagacc
     3601 tgggacgcca tcggcgtgaa ggaatccgtc gagaagatgg agaaggacgg gttcaaggtg
     3661 cccgagaagg tcaagaagat gctcgccaag ggcgccactt cgttctacaa gctcgagaac
     3721 ggcaagaaga tgttctggga cttcgcgagc gagacctaca agcccgtgaa gacaagcccc
     3781 aaggcgctgc agctctccaa cttcaaggcc gagaacaaga cggtgttgac ctcgggttcg
     3841 tgctcgctca tcgacatcgg cgacggcgtg ttctgcctcg agttccactc caagatgaac
     3901 gccatcaaca aggagatggt cgagttcatg gccaaggccg gcgagtacgt cggcgccaac
     3961 ggcgtgggca tggtcatcgg caaccaggcg ggcggcatgc cgggcgcgtt ctccgcgggc
     4021 ggcgacctcg cctacatgct cgggctcgcc agggccggca agttcagcga gatcgacgcg
     4081 ttcatccgtg aggtccacaa gggcatcatg gcgacccgct acagcaacta ccccgtggtc
     4141 gcggcaccct acggtctcac cctcggcggc ggctgcgagg tgtgcctgtc cgctgaccgc
     4201 atcgtggcgc acgccgacct gttcatgggt ctcgtcgaga tcggcgcagg tctcgtcccc
     4261 ggcggctgcg gcatgatcaa cctctggaga aagttcatca catccaagcc ggaagacgcg
     4321 aaggtcaccg attacggcgg attcttcctc aacgcgttca tggccgtggc gcaggccaag
     4381 gtgtccaact ccgccgaaca ggcccgccag cgcgggttcc tcggcgcggc cgaccgcatc
     4441 gtcatgaacc gcgacaacct catcggagag gccaagaaag aggtcctcaa gatggtcgac
     4501 gacggctacg ccccgccgat caagaagaag atcccggtgt tcggccgcga ggctcacggc
     4561 atggtctggg cggagatgaa caacatgaag ggcggcggct acataactcc tcacatggag
     4621 ttcatcgcca agaagatctg tatctgcatg tccggcggcg acgtgccctg cggcgcggag
     4681 gtgaccgaag agcacctcat gacgctcgag cgcgaggcgt tcgtcgagct gtggaagacg
     4741 gagaacacgc agaagatggc cgagcacatc atgaacacgg gcaagccgtt gttcctgtaa
     4801 tcggataaca cgagaaagaa cgaggaggaa accaatgaga gaagcatata tcgttcagtc
     4861 ggtgaggact ccgggttgca gaagggccag gggcgccttt gcccagacca gacccgagga
     4921 tctcctcaag gcggccctca acggcctcat ggagaggacc ccgggcgtcg agaagaagga
     4981 cgtggaggac atcctcgtcg ggtgcgcctt ccccgaggct gagcaggggc tcaacatcgg
     5041 ccgcatcatc gcccagatgt gcaagttccc ggacgacacc tgcggcgcca cggtcaatag
     5101 gttctgcgca tcgggtctcg aggcgatcag cctccaggcc atgaggatca tggcgggcat
     5161 gtgcgacgtc gccatcggcg gcgggctcga gtccatgtcc atcgtgccca tgggcggcaa
     5221 catgccgagg cctcacccgg attgggccaa ggaaaaccct gacgtctaca tctccatggg
     5281 catgacggca gagaacgtgg cgaaccgcta caagatcacc cgcgagatgc aggaccgctt
     5341 cggctactct tcccagatga aggccgcaaa ggcccagaag gaagggaagt tcaaggagat
     5401 cgtgccggtg gaggcggtga ggttcaagga gaaggacggc atcctggtca aggagacctt
     5461 catccaggac ttcgacgacg gcgtgagggc cgacaccacc atcgaggggc tctccaagct
     5521 caggccggcg ttcgctgcca tgggttcggt cactgcgggc aactcctcgc agaccaccga
     5581 cggtgcggcg gcaacgctcc tcatgagcgg cgaagcggtc aagaagttcg gcgtgaagcc
     5641 catcgccaag ttcaaggcct acgtggtcgc aggctgcagg gccgacgaga tgggcgtggg
     5701 gccggcgtac gccatcccca agctcctcaa ggtaacaggg ctcggtacgg ccgctgacat
     5761 cgccaaggac gccatcttcg aaatcaacga ggccttcgcg tcccaggcca tctactgcct
     5821 gcagcagatc ggcctctacg atgacaagta ccactggttc gagagcgacg agcgcatcgt
     5881 caacgtcaac ggcggggcca tcgcgctcgg ccacccgctc ggctgcaccg gcgccaagct
     5941 ctgcgcccag ctcatgaccg agatgcaggc ccgcaagaag aagtggggca tcgagtccat
     6001 gtgcatcggc ggcggcatgg gcgctgcggc actgttcgag ctctgcgact aagagcggat
     6061 tcacggaaag ggaacacgaa ggggctgccg ccgaaaacgg cggcaccccc ttttcgtctt
     6121 cagtctctcg gggggcccgg aaacaggtct tgccatggag atcgacccgg tcgatgaccc
     6181 tcgtggcctg aaggtctctc gcatacttca cctgctcgct ccggtggata tcgagtctgt
     6241 ctgcattgga ccccaatgcg gcgccctgta gggtggataa cgtaaaatat ttttcgcact
     6301 ttctggtttg tcattgaaaa aggtcc
//
LOCUS       DAOSCR010000071         6214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8030_length_6214_cov_3.407047, whole genome shotgun sequence.
ACCESSION   DAOSCR010000071 DAOSCR010000000
VERSION     DAOSCR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8030_length_6214_cov_3.407047"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PLT69_03005"
     CDS             <1..1276
                     /locus_tag="PLT69_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ13444.1"
                     /translation="FCTKKCRRGQLDEPMAIAWIKRFIADNAKRPKIDQVPVTRKQKI
                     AVIGAGPAGLTAAQDLCLRGYKVTVFEELPHAGGMMRYAIPEYRLPRNVIEQEVDDIR
                     ALGVEIKTNTRVGRDVSFEQIRKDYDAVVIAVGAHKSWKLDIEGEDLDGVWGAVEFLR
                     EVNLGKKVGIGKRVAVVGGGNSAIDAARTAVRLGAKDVTILYRRERKDMPAWENEIVA
                     AEHEGVKIEYLVAPVRLIGKNGKLVGVECQRMRLGEFDRSGRRKPIPIQGSEFTLAVD
                     TIIPAISQNTDASFIPAGSDVALDKWGGIKTEGRAKNRTSAEGVFIAGDAASGPATVV
                     EAIDMGHKTAIDVDAYIRAKNNEPPYKEPPEETIEIHFEVDEEVVETPRAKMPELKKS
                     ERMISFKEVELGYSKKAAHREACRCLRCDAEL"
     gene            1301..3070
                     /locus_tag="PLT69_03010"
     CDS             1301..3070
                     /locus_tag="PLT69_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOJ13445.1"
                     /translation="MATVQVTIDNVKINVEEGSTILEAAQAGGISIPTLCAWPEIGHT
                     PGACRVCVCEVEGQRNLVASCVFPVSDGMVVKTTTERVRKARRLNVELLLANHPTDCN
                     VCIRNGNCELQKVAEAVGLRSVRFDYPAGKERVIDMSSPSIVRDPGKCIKCYRCVTVC
                     ENIQTANVLQPSYRGDKVFIGPPFGLPLIETNCTMCGQCVLACPVGALYEKDDTEAVW
                     AAIEDPKKHVVVQEAPAIRAALGEEFGLPVGSLVTGKMITALKRLGFDKVFDTNFTAD
                     LTIMEEGSELLQRVKEGGKLPMITSCSPGWIKFCEHFYPDLLDHLSTCKSPQQMMGAL
                     IKSYYAQQAGIDPKDIVSVSIMPCTAKKFECVRDEMDDSGYQDVDYVLTTRELARMIR
                     QAGIDFANLPDGEYDNPLGEYTGAGTIFGATGGVMEAALRTVYAIVTGTNLESLDVTP
                     VRGLEGVKEASVEVGELGKVKVAVAHGLANARKLMDLIREGKADYAFIEVMCCPGGCV
                     SGGGQPIAPSNEKRIKRASALYQDDAAVQKYRQSHENPSVKRIYEVFLGKPLGHKSHE
                     LLHTHYTKRGTDVPHKKVNIKRT"
     gene            3205..3477
                     /locus_tag="PLT69_03015"
     CDS             3205..3477
                     /locus_tag="PLT69_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOJ13446.1"
                     /translation="MTERTSMVDLSRIGVVSIFIHDRLAQSSRVNEILSAHGEIVVGR
                     MGIPYREAGVHVIALIIHGTTDEIGALTGKLGSIRGVEVKSALARN"
     gene            3488..4882
                     /gene="hydG"
                     /locus_tag="PLT69_03020"
     CDS             3488..4882
                     /gene="hydG"
                     /locus_tag="PLT69_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOJ13447.1"
                     /translation="MEDFIRDDIIEQTLDRARNPEPSRVRDVIGKALELQGLTPGEAA
                     VLLQCEDPELTGEILEAAHRVKLEIYGNRLVIFAPLYISNYCSNNCLYCGFRRDNTEL
                     KRRSLTLEEIADEVMALEREGHKRILMLCGEHPGMSPLDYFIEAIETVYATTTEKGGE
                     IRRINVEIAPMSVEDFRRLKAAKIGTYVLFQETYHHETYRRMHPVGPKADFLWRLTAQ
                     DRAMQAGIDDVGIGALFGLYDYKYEVIGLLHHALHLDETYGVGPHTISVPRLEPALNA
                     PAAIQPPSPVSDDDFKKLVAVIRLSVPYTGMILSTREPAELRSVVFDLGISQISAGSR
                     TNPGGYRKGDSNDFRAAQFNLGDTRTLDEVIYDITQRGHIPSFCTACYRLGRVGKNFM
                     DLAKPGLIQKYCRTNAILTFAEYLADYASPETKKAGDALVDRLVAETEEKAMRELLEE
                     RVQAVRGGAHDLYI"
     gene            4866..5924
                     /gene="hydE"
                     /locus_tag="PLT69_03025"
     CDS             4866..5924
                     /gene="hydE"
                     /locus_tag="PLT69_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOJ13448.1"
                     /translation="MTSTSEAMDTGDLIARAEEGLGLARPEIVHLLSLDDPAQVEALY
                     QAADRVRRRCVGDEVHLRGVIEFSNHCRNNCTYCGLRRDNAKLARYRMEDDEIVECAL
                     GIAEGPCGTVVLQAGEDPAFTAERLCGIISAIRARTPLAVTLSVGRWPYEDLKAFRDA
                     GASRYLLRHETANASLYESLCPGRRLSDRIECIRHLKRLGFETGMGCMVGLPGQTVED
                     LASDILLMQEMDADMIGIGPFIPHGDTPLAGHPPGDAEMVLKMLAVTRLVTRDTNIPA
                     TTALGVLRAEARKRAFSTGANVFMPVYTPKKYAPDYQIYPGKAVSVHEDMPLQEVVDY
                     FSSIGRPIGAGHGARRKR"
     gene            5921..>6214
                     /locus_tag="PLT69_03030"
     CDS             5921..>6214
                     /locus_tag="PLT69_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOJ13449.1"
                     /translation="MRTERDALGPLDLPDDCLYGIHTVRALVNFGTGPERHDREFVRA
                     YLLVKKACALANAELGYLDRARAQALTAAIDSLIDQGRFDDVVVNPVSGGAGTS"
BASE COUNT         1314 a         1903 c         2090 g          907 t
ORIGIN      
        1 gttctgcacg aagaagtgcc gcaggggcca gctcgacgag cccatggcca tcgcctggat
       61 caagcgtttc atcgcagaca acgcgaagag acccaagatc gaccaggtgc cggtgacgcg
      121 caagcagaag atcgcggtca tcggcgcagg gcctgccggc ctcaccgcgg cccaggatct
      181 gtgcctgcgg gggtacaagg tcacggtgtt cgaggagctc ccgcatgccg gcggcatgat
      241 gcgctacgcc atcccagagt accgtctgcc caggaacgtc atcgagcagg aggtggacga
      301 catccgggcg ctcggcgtgg agatcaagac gaacacccgc gtgggaaggg acgtctcctt
      361 cgagcagatc cgcaaggact acgacgccgt ggtcatcgcc gtgggcgcac acaagagctg
      421 gaagctcgac atcgagggcg aagacttgga cggcgtctgg ggagccgtcg agttcctccg
      481 cgaagtgaac ctgggcaaga aggtcggcat cggcaagcgc gtggcagtgg tcggaggcgg
      541 gaactcggcc attgacgcgg cccgcacggc ggtgcgcctc ggagccaagg acgtcacgat
      601 cctctaccga cgcgagcgca aggacatgcc cgcatgggag aacgagatcg tggcggcgga
      661 gcacgaaggt gtcaagatcg agtacctcgt ggcccccgtg aggctcatcg gcaagaacgg
      721 caaactcgtg ggcgtcgagt gccagcgcat gaggctcggc gagttcgacc ggtcgggcag
      781 gcgcaagccc atccccatcc agggcagcga gttcacgctg gcagtcgaca ccatcatccc
      841 cgccatctcc cagaacacgg acgcctcctt cataccggcg ggctctgacg tggccctcga
      901 caagtggggc gggataaaga ccgaaggcag ggcgaagaac aggacgagcg ccgagggcgt
      961 gttcatcgcg ggtgacgcgg cgagcggtcc cgcgacggtg gtcgaagcca tcgacatggg
     1021 ccacaagacg gcgatcgacg tggacgccta catcagggcg aagaacaacg agccgcctta
     1081 caaggaaccg cccgaggaga ccatcgagat ccacttcgag gtcgacgagg aggtggtgga
     1141 gacacccagg gcgaagatgc cggagctcaa gaaatccgag aggatgatct ccttcaagga
     1201 ggtggagctc gggtactcca agaaggcggc gcacagagag gcttgcaggt gcctgcgctg
     1261 cgacgcggag ctgtagaggc ggcaagaagg agaagacacc atggcaaccg tacaggttac
     1321 gatcgacaac gtgaaaatca acgtggaaga gggcagcacc atcctggaag cggctcaggc
     1381 cggcggtatc tccatcccta cgttgtgcgc atggcctgag atcgggcaca cgcctggcgc
     1441 gtgcagggtc tgcgtgtgcg aggtggaggg gcagagaaac ctcgtggcct cctgcgtgtt
     1501 ccccgtgtcc gacggcatgg tcgtgaagac caccaccgag cgggtgcgca aggcgcgcag
     1561 gctcaacgtc gagctgcttc tggccaacca cccgacggac tgcaacgtgt gcatacggaa
     1621 cgggaactgc gagctccaga aggtcgccga ggccgtcggg ctgagatccg tcaggttcga
     1681 ctaccctgcc gggaaggagc gggtcatcga catgagcagc ccgtccatcg tgcgcgaccc
     1741 gggcaagtgc atcaagtgct accggtgcgt gaccgtgtgc gagaacatcc agaccgcaaa
     1801 cgtgttgcag ccctcttaca ggggcgacaa ggtcttcatc gggccgccgt tcgggcttcc
     1861 cctcatcgag accaactgca ccatgtgcgg ccagtgtgtg ctcgcctgcc ccgtgggtgc
     1921 gctctacgag aaggacgaca ccgaggccgt ctgggcagcg atcgaggacc cgaagaagca
     1981 cgtcgtcgtg caggaggccc cggccatcag ggcggccctc ggagaggaat tcggtctccc
     2041 ggtgggctcc ctcgtgacag ggaagatgat cacggccctc aagaggctgg gcttcgacaa
     2101 ggtgttcgat acgaacttca cggcggatct caccatcatg gaagagggga gcgagctgct
     2161 ccagagggtc aaggagggag gcaagctccc catgatcacc tcgtgctcgc ccggctggat
     2221 caaattctgc gagcacttct acccagacct gctggatcac ctctccacct gcaagtcgcc
     2281 ccagcagatg atgggcgcgc tcatcaagtc ctactacgcg cagcaggcgg gcatcgaccc
     2341 gaaggacatc gtgtcggtct ccatcatgcc ctgcacggcg aagaagttcg agtgcgtgcg
     2401 tgacgagatg gacgacagcg gctaccagga cgtcgactac gtcctgacca cccgcgagct
     2461 cgcccgcatg atcaggcagg cgggcatcga cttcgccaac ctccccgacg gagagtacga
     2521 caacccgctc ggcgagtaca cgggagcagg caccatcttc ggggcgacgg gcggcgtcat
     2581 ggaggcggcc ctcaggaccg tctacgccat cgtcaccggc accaacctcg agagcctcga
     2641 cgtcacgccg gtcaggggcc tggaaggcgt caaggaggcg tccgtcgagg tgggcgagct
     2701 gggcaaggtc aaggttgcgg tcgcccacgg cctcgcaaac gccaggaagc tcatggacct
     2761 catccgtgag ggcaaggccg actacgcgtt catcgaggtc atgtgctgcc ccggcggctg
     2821 cgtctccggc gggggccagc ccatcgcgcc gagcaacgag aagaggatca agcgtgcgtc
     2881 agccctgtac caggacgacg ccgccgtaca gaagtacagg cagtcgcacg agaacccatc
     2941 ggtcaagagg atctacgagg tcttcctcgg aaagcccctg gggcacaagt cccacgaact
     3001 cctccacacg cactacacga agaggggaac cgacgtgcct cacaagaagg tgaacatcaa
     3061 gaggacctga gacgacgcac cagagaaacg acacctgcgg gggaggtcac gaggctgacc
     3121 tcaagggtgg gggagaatcg acgatcatga cggtccccgc ccgtctgcgc gggcaggggc
     3181 cgtcacactt tttcaggagt cgacatgact gagcggacga gcatggtgga tctgagcagg
     3241 atcggcgtgg tgagcatctt catccacgac aggctggccc agtcgtcgcg cgtcaacgag
     3301 atcctgagcg cgcacggcga gatcgtggtg ggaaggatgg ggatccctta ccgggaggcc
     3361 ggggtgcacg tgatagcgct catcatccac ggaaccacag acgagatagg cgcactgacc
     3421 gggaagctgg ggtcgatcag gggagtggaa gtgaagtcgg cgctggcgag aaactgagga
     3481 gagggtcatg gaagacttca taagggacga catcatcgaa cagaccctgg acagggcgag
     3541 gaacccggag ccctcgcgag ttcgtgacgt gatcggcaag gcgctcgaac ttcaggggct
     3601 cacaccggga gaggccgccg tgctgctcca gtgcgaggac ccggagctca ccggggagat
     3661 cctggaggcg gcgcaccgcg tgaagctgga gatctacgga aacaggctgg tgatcttcgc
     3721 cccgctctac atctccaact actgctccaa caactgcctc tactgcggct tcaggaggga
     3781 caacacggaa ctcaagaggc gctcgctgac cctcgaggag atcgcggacg aggtgatggc
     3841 cctggagcgc gagggacaca agcggatcct catgctctgc ggcgagcacc cagggatgag
     3901 cccgctcgac tacttcatcg aggccatcga gacggtctat gccacgacca cggaaaaggg
     3961 cggggagatc aggcgcatca acgtggagat cgccccgatg agcgtcgagg acttcaggag
     4021 gctcaaggcg gcgaagatcg gcacctacgt gctcttccag gagacctacc accacgagac
     4081 ctacaggagg atgcaccctg tcgggccgaa ggccgatttc ctctggaggc tcacggcgca
     4141 ggacagggcc atgcaggcgg ggatcgacga cgtggggata ggtgcgctgt tcggcctgta
     4201 cgactacaag tacgaggtca tcggcctcct ccaccatgcc ctgcatctcg acgagaccta
     4261 cggcgtggga ccacacacga tatccgtgcc gaggctcgag ccggccctca acgcgccggc
     4321 tgccatacaa cctccctcgc ccgtgagcga cgacgacttc aagaaactcg tcgcggtgat
     4381 caggctgagc gtcccgtaca ccggcatgat cctgtcgacc agggagccgg ccgagctcag
     4441 gagcgtggtg ttcgacctgg gcatctcgca gatcagcgcg gggtccagga ccaacccggg
     4501 cgggtaccgc aagggcgaca gcaacgactt cagggcggcg cagttcaacc tcggcgacac
     4561 caggaccctc gacgaggtga tctacgacat cacccagagg ggacacatac cgagcttctg
     4621 cacggcgtgc taccgcctcg gcagggtggg caagaacttc atggatctcg ccaagcccgg
     4681 cctcatccag aagtactgcc gcaccaacgc gatactcacc ttcgccgagt acctcgcgga
     4741 ctacgcgagc cccgagacga agaaggccgg ggacgccctg gtggacaggc tcgtggccga
     4801 gacggaggag aaggccatga gggaactcct cgaggaacgc gttcaggccg tgcgcggggg
     4861 ggctcatgac ctctacatct gaggccatgg acaccggcga tctcatcgca cgggccgagg
     4921 aaggcctggg gctggcaaga cccgagatcg tgcacctcct gtccctcgac gaccccgctc
     4981 aagtcgaggc cctctaccag gcggcagacc gagtgagacg caggtgcgtg ggagacgagg
     5041 tccatctcag gggagtcata gagttttcaa atcactgcag gaacaactgc acgtactgcg
     5101 gcctcaggcg ggacaacgcc aagctcgcca ggtacaggat ggaagacgac gagatcgtcg
     5161 aatgcgccct gggcattgct gaaggtccct gcggcaccgt ggtgctccag gcgggcgagg
     5221 accccgcctt cacggcagag aggctgtgcg ggatcataag cgcgatcagg gcgaggaccc
     5281 cgcttgccgt caccctgtcg gtcggccgtt ggccgtatga agacctcaag gccttccggg
     5341 acgcgggtgc gagccgctac ctgctcaggc acgagaccgc gaatgcgtcc ctgtacgagt
     5401 ccttgtgccc gggcaggagg ctctcggaca ggatcgagtg catcaggcac ctcaagcgtc
     5461 tcggcttcga gacgggcatg gggtgcatgg tggggctgcc gggccagacc gtcgaggacc
     5521 tcgcctcgga catcctcctc atgcaggaga tggatgcgga catgataggg atcggaccgt
     5581 tcatcccgca cggggataca ccgcttgcag gacatccacc gggagatgcg gagatggtcc
     5641 tcaagatgct ggctgtcacg aggctggtga ccagggacac gaacatcccg gcgaccacgg
     5701 ccctcggggt gctgcgcgcc gaggccagga agagggcgtt ttccacaggc gcgaacgtgt
     5761 tcatgccggt ctacaccccg aagaagtacg cccccgacta ccagatctat cccggcaagg
     5821 cggtgtcggt gcatgaagac atgccgttgc aagaggtcgt cgactacttc tcttccatcg
     5881 gcaggccgat aggcgccggg catggcgcaa ggaggaagag gtgagaacgg agcgtgacgc
     5941 actgggcccg ctggatctcc cagacgactg cctctacggt atccacacgg tgagggccct
     6001 cgtgaacttc gggaccggac ccgagcgtca tgacagggaa ttcgtgcgcg cctacctcct
     6061 cgtgaagaag gcgtgcgccc tggcgaacgc ggagctcggg tatctcgaca gggcgagggc
     6121 ccaggcattg accgccgcca tagactcgct gatcgatcaa ggcaggttcg acgacgttgt
     6181 cgtgaaccct gtctcgggag gcgccggcac ctcc
//
LOCUS       DAOSCR010000072         6177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8088_length_6177_cov_3.156648, whole genome shotgun sequence.
ACCESSION   DAOSCR010000072 DAOSCR010000000
VERSION     DAOSCR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8088_length_6177_cov_3.156648"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLT69_03035"
     CDS             complement(<1..680)
                     /locus_tag="PLT69_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13450.1"
                     /translation="MNVCNLCPRRCGVDRSRARGACRSDDRMKIGAAVIHRGEEPPLV
                     HGAGSGAVFFAGCPMRCSYCQNRQISQLCRGRTVHPPELAAIMLDFQEAGCSNVNLVS
                     PTHYSPWVLESLGIARDRGLKIPVVVNSGGYESVECLDMWAGQDCIYLVDLKYGDNDT
                     GVRLSGVEGYWDAARKAVRHLYETSGPLVLDGEGSAVRGLLVRHLVLPGMRSNPFAVL
                     EFLADLSR"
     gene            complement(677..2161)
                     /locus_tag="PLT69_03040"
     CDS             complement(677..2161)
                     /locus_tag="PLT69_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13451.1"
                     /translation="MNLYSWLVDHARMRPEKVAIRTRDLSITYGELFRLTCALKESLR
                     KAGIGEDDHITVMLPNIPEFVVSYMAVVGLGAVVTPVNPAYTSRELRHILVDSDSKGL
                     VIEQSNIETYGAIARECPQQVLITVGKEGTFSRWVGGGADESVVDRDVDDVAVMIYSS
                     GLTGYPMGAMLTQGNLDHNSDLMRICMEADDRDTTLTVIPCFHSFSASVNMLSMLRYG
                     GTVYLMRKLDFKDLRHALTDGGVTAVCAVPTLFYGLVHHPELGDVDYSRMTTLIAGGS
                     ALPLEVYESFREKFGVEIRQGYGITEASPVCSVNQKYRPIKPTSIGQTVPGVKVRVQA
                     EDGRVLGPNEVGELLFCGPNIMKGYYKKERETQEIIEDGWLHTGDLGYVDEDGYIFIT
                     GYKKDMVITSGFNVYTKEVTNVLNSLPGVKDSAVVGEPDLMRGAIIKAYVVRSTPELT
                     EDEVKRFARMQLATYKTPRKVVFVPEIPRDSSGKVIADLLERGS"
     gene            complement(2568..2828)
                     /locus_tag="PLT69_03045"
     CDS             complement(2568..2828)
                     /locus_tag="PLT69_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13452.1"
                     /translation="MDFRAEHCLWCPAKAEPVQGCASCPRYRRYFLHLVVEFTAEAFC
                     SMCIHNGGGELEVFCRTNRALQAGSPEDFDCYKFSPARPRLL"
     gene            complement(2941..5196)
                     /locus_tag="PLT69_03050"
     CDS             complement(2941..5196)
                     /locus_tag="PLT69_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOJ13453.1"
                     /translation="MSRATRLALFCMAALAVAALAALAAASLDAYRTMSRLASASVWR
                     LPMKVYSRPFVLQPGVDISREGLPARLTRLEYRPSPECRRPGDYRITGSTLEVFLRAC
                     NRPGMSTRPLRVRIVLEGSKISSIVSPETGRPLSSAVLEPEVVATLYDEGFEDREVMT
                     LAECPRVLLDAVLCVEDRRFYSHAGIDVRALLRALVADLSGRRIAEGGSTITQQLVKN
                     LFLSGERTFSRKIREAWLALIMEAALSKDEILTLYLNEVYLGRYGSASIHGFKRASKI
                     FFDKDPKDLELHEAALLAGLIRSPNRTSPYRQPAQALLRRNTVLELMADNDSVPRDLC
                     TRAMAMPLGVVPYAHPARRAPYFVDIVLERVREVGKGDDLLAKGGLHIYTTLDMQVQS
                     AAEEAVRSSARRLGDQVQAACVVLDPKTCEVLAMVGGRDYRSSQFNRAVRLRRNIGSL
                     MKPVVYYAALSGTYTLASMVDDAPVEVRLADGSRWTPENQDSTSHGTVMLMDALVHSY
                     NQATVRLALDCGLERVRSIAGDLLPPGSLSQGPSFVLGAARCSPLDVAVMYAAFASGG
                     VRRPPSCLDAVCDSRDVVIHEETPAGEARVLDERVVFLVNYALEQVVERGTASRARRL
                     GVPQGVCGKTGTTNGMCDSWFAGFTPDIVVAAWVGRDDAKSVGLTGADGAMPIAAGVL
                     AKAASVRQREAPEGIAFCEVDPASGTRADAFTRDPVRVPFIEGREPPEPSGGVLPSLW
                     KALRGLLPFGT"
     gene            complement(5750..>6177)
                     /locus_tag="PLT69_03055"
     CDS             complement(5750..>6177)
                     /locus_tag="PLT69_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ13454.1"
                     /translation="YGESEPSGQPARRGALLEQHLWEMDRKLDLIINMVISEDFKALV
                     RTIPREVNISASGMRFISAEPYDIGDILELHIILPMRPLLMVNLIGEVLRLKPLASTP
                     DVNRWAVAVRFHRVDDDTREDIIRYLFRRQREVLRQRQP"
BASE COUNT         1028 a         2123 c         1984 g         1042 t
ORIGIN      
        1 tccctcgaca ggtcggccag gaactccagc acggcgaagg ggttggatcg catgccgggc
       61 agcaccaggt gcctgaccaa gagccccctg acggcggatc cctccccgtc gagcacgagc
      121 ggccccgagg tctcgtagag gtggcgcacg gccttcctgg cagcgtccca gtacccttcc
      181 accccgctca gccgcacacc ggtgtcgttg tccccgtact tgaggtcgac gaggtagatg
      241 caatcctggc ccgcccacat gtcgaggcac tccaccgact cgtatccacc ggaattgacc
      301 acgaccggga tcttgaggcc cctgtccctc gcgatgccca aggattccag cacccacggc
      361 gaatagtggg tcgggctcac gaggttcacg ttgctgcacc ccgcctcctg gaaatccagc
      421 atgatggcgg caagctccgg ggggtggacg gtccttccgc ggcagagctg gcttatctgc
      481 ctgttctggc agtaggagca cctcatgggg cagcccgcga agaacacggc gcccgagccc
      541 gcaccgtgga cgaggggcgg ttcctccccc ctgtggatga cggcggcccc gatcttcatg
      601 cggtcgtcgg acctgcaggc gcccctcgcc ctcgacctgt ccacgccgca cctcctgggg
      661 cacaggttgc agacgttcat gagcctcgct cgaggagatc cgcaatgacc ttgccggagg
      721 aatccctggg gatttccggg acgaagacca ccttcctcgg ggtcttgtag gtggcgagct
      781 gcatgcgggc gaagcgcttg acctcgtcct cggtgagctc cggggtcgag cggaccacat
      841 aggcctttat gatggccccg cgcatgaggt cgggctcccc caccacggcg ctgtccttca
      901 cgccgggcag gctgttgagg acgttcgtca cctccttggt gtacacgttg aacccggatg
      961 tgatgaccat gtccttcttg tagcccgtta tgaagatgta gccgtcctcg tcgacgtagc
     1021 cgagatcacc cgtgtggagc cacccgtcct cgatgatctc ctgtgtctct cgttccttct
     1081 tgtagtaccc cttcatgatg ttcggcccgc agaagagcag ctcgcccacc tcgttcggac
     1141 ccaacaccct cccgtcctct gcctggacac gcaccttgac gccgggcacc gtctggccga
     1201 tggaggtggg cttgatcggc ctgtacttct ggttcaccga gcacacgggg gatgcctcgg
     1261 tgatcccgta gccctgcctg atctcgacgc cgaacttctc cctgaaagac tcgtagacct
     1321 ccagggggag cgcggagccc ccggctatca gggtggtcat gcggctgtag tcgacatcgc
     1381 cgagctccgg gtggtgaacc aggccgtaga agagggtcgg cacggcgcag acggccgtaa
     1441 cgccgccgtc ggtgagcgcg tggcgcaggt ccttgaagtc gagcttcctc atgaggtaga
     1501 cggtcccgcc gtagcggagc atggagagca tgttcacgct cgcactgaac gagtggaagc
     1561 aggggatgac ggtgagcgtc gtatccctgt catcggcttc catgcatata cgcatgaggt
     1621 cggagttgtg gtccaggttt ccctgcgtga gcatggcgcc catggggtac ccggtgaggc
     1681 cggacgagta gatcatgacc gccacgtcgt ccacatcgcg gtccacgacc gactcgtcag
     1741 ccccgccccc cacccagcgg ctgaaggtcc cctccttccc gacggtaatg aggacctgct
     1801 gggggcattc gcgggcgatc gccccgtacg tctcgatgtt cgactgctcg atgacgagac
     1861 ccttcgagtc ggaatcgacg aggatgtgcc tcagttcccg ggaggtgtag gccgggttca
     1921 cgggggtgac cacggcaccc agcccgacca ccgccatgta gctcaccacg aactcgggaa
     1981 tgttgggcag catcactgtg atatggtcgt cctcgccgat ccccgccttc ctgagggatt
     2041 ccttgagggc gcaggtaagc ctgaagagct cgccgtaggt gatcgagagg tccctggtac
     2101 ggatcgccac cttttccggc ctcattctgg cgtgatccac gagccacgag tagagattca
     2161 tgctcccccc tgtcatcaaa gtgacaccac agtgccgttc gccccgtaac gaaacaccac
     2221 gtactacatt cacggatcag ggttcaacag gtttcctccc accggcactc cacgcgacgg
     2281 gccgtcactc ccgccacgcg gccatggcgc ctcgaaggcg ccgcttgaag aggcccgcgc
     2341 tttttcgccg gcccgacgga cgcacaaccc cgacaccgtt ctgcaaggcc gtctcccacc
     2401 cggtgcggat gggcccgccc ttgttctcga ggcccgcctg ccgccctgca tacgcggccc
     2461 ggcaaccagc tgcaaaccat caccgagggg tttccggctg gtatccgccg gacaccccac
     2521 gcggccgctt cccgacaccg gccgggcgcc ggccgggata cgccccgtca caagaggcgc
     2581 ggccgggcag ggctgaactt gtagcagtcg aaatcctccg ggctgcccgc ctgcaatgcc
     2641 ctgttggtcc tgcagaacac ctcgagctcg ccccccccgt tgtggatgca catcgagcag
     2701 aaggcctcgg ccgtgaactc gacgacgagg tgcaggaaat acctccggta cctggggcag
     2761 gaggcgcacc cctgcacggg ctcagccttc gcggggcacc acaggcagtg ttcggccctg
     2821 aagtccatgt gcccaccata cggctcatcc gcctcgccct caagcgaaaa gtcgcaccga
     2881 ggcccggggc gccaccctca gcctcccaca ggagaacggg aggcctttcc gcccgaagca
     2941 tcacgtgccg aagggcagga ggcccctgag cgccttccag agagagggga ggaccccccc
     3001 ggatggctcg ggcggttccc tgccctcgat gaaaggcacc ctcacggggt cgcgggtgaa
     3061 ggcgtcggcc ctggtgccgc ttgcgggatc gacctcgcag aacgcgatcc cctcgggggc
     3121 ctctctttgc ctcaccgagg ccgccttggc gaggacgccc gccgcgatcg gcatggcgcc
     3181 gtccgccccc gtgagcccga cgctcttcgc gtcgtccctg cctacccacg cagcgaccac
     3241 gatgtccggg gtgaaccctg cgaaccacga gtcgcacatg ccgttggtgg tcccggtctt
     3301 accgcacacc ccctgcggca cgccgaggcg tcgagccctc gaggccgtgc ccctctccac
     3361 cacctgctcc agcgcgtagt tcacgaggaa caccaccctc tcgtccaaga ccctggcttc
     3421 gccagccggc gtctcctcgt ggatcacgac gtccctcgag tcgcagactg catcgaggca
     3481 cgacggcggc ctcctgacac ccccggatgc gaaggccgcg tacatgaccg ccacgtcgag
     3541 gggggagcac cgggcggcgc cgagcacgaa ggaaggcccc tgggacaggc tgccgggggg
     3601 caggagatcc ccggcgatag acctcacccg ctcgaggccg cagtcgagcg cgagccttac
     3661 cgtggcctgg ttgtaggagt gtacgagggc gtccatgagc atgaccgtgc cgtggctggt
     3721 cgagtcctgg ttctcgggcg tccacctcga gccgtccgcg aggcggacct cgacgggcgc
     3781 gtcatccacc atgctcgcga gcgtgtaggt ccccgagagg gcggcatagt agacgacggg
     3841 cttcatgagc gacccgatgt tcctcctcag gcgtaccgcc ctgttgaact gggacgagcg
     3901 gtaatccctg ccgcccacca tggcgaggac ctcgcaggtc ttcgggtcca gcaccacgca
     3961 cgcagcctgg acctgatcgc ccaagcgtcg cgccgaggaa cggacggcct cctccgcggc
     4021 agactggacc tgcatgtcca gtgtcgtgta gatgtgcagg ccgcccttgg cgagaaggtc
     4081 gtctcccttc cccacctccc gaaccctctc cagcacgatg tcgacaaagt agggcgcacg
     4141 cctggccggg tgggcgtacg ggacaacccc gagcggcatg gccatggccc gtgtgcacag
     4201 gtccctgggg acggagtcgt tgtccgccat gagttcgagc acggtgttgc gtctcaggag
     4261 cgcctgggca ggctgcctgt agggcgacgt gcggttcggg gagcggatga gcccggccag
     4321 gagggccgcc tcgtggagct cgaggtcctt cgggtccttg tcgaagaaga tcttgctcgc
     4381 ccgcttgaaa ccgtggatgc tcgcgctgcc gtaccgcccg aggtaaacct cgttgaggta
     4441 gagggtgagg atctcgtcct tcgacagggc cgcttccatg atgagcgcca gccaggcctc
     4501 cctgatcttc cgggaaaacg tcctttcacc ggagaggaag aggttcttga cgagctgctg
     4561 ggtgatggtg gacccgcctt cggcgatcct tctccccgag aggtcggcga cgagggcccg
     4621 gaggagggcc cgcacgtcta tgccggcgtg gctgtaaaac ctcctgtcct ccacgcacaa
     4681 gaccgcgtcc aggaggacgc gggggcactc ggcaagcgtc atgacctccc tgtcctcgaa
     4741 cccctcgtcg tacagggtgg ctacgacctc gggctcgagc accgccgacg acagcggcct
     4801 gcctgtctcg ggcgagacga tggacgagat cttcgagccc tcgagcacga tgcgcacccg
     4861 taaaggccgc gtggacatgc ccggcctgtt gcatgcccgc aggaagacct ccagggtcga
     4921 gcccgtgatc ctgtagtcac ctggccgcct gcactcgggt gacggccggt actcgaggcg
     4981 cgtgagcctg gcggggaggc cttcacgcga gatgtccacg ccgggctgga gcacgaacgg
     5041 cctgctgtag accttcatag gaagccgcca caccgatgcg ctcgccagcc tcgacatggt
     5101 gcggtaggcg tccagcgagg cggctgccag ggcggcaagg gccgcgacgg cgagagctgc
     5161 catgcagaac aaggcaaggc gcgtggctcg tgacacgatt accctctata ggcccgacgg
     5221 tcaggcccgt caatccgtgg ggccgcccca cccgggggat ccccctgtcg gagtcggcca
     5281 tggatgcagc ccttctcgaa ggccggccaa cccttacgac cacccgggat cccggggtct
     5341 atccgcatac cgtcagcccc cctcctgcgc accgggtata gtgaggactc cccgctcatg
     5401 aggacctcgg gctggaggcc tgggccttga tttcttccca ggcgaggaat ccccgctcat
     5461 gaggacctcg cgcgcgttct ctcctgagcg tcttcccgca cggcgaatct cgaaggctgg
     5521 accccacggc gcacccgaca ggcggcgcca cggggtgcgc ggtgtcgtgc ccttggatgg
     5581 gctggacgct gggggttcgg gcccgagggc gacgcgtgca cgaacggcag ggtcacgaac
     5641 cgctttgctg gggaaagcgg cctaggcatt gcgtgcaggg atacggggac ttcctcgcgg
     5701 ggtgcagcac gacgcgcgca ggcccgccga agccgcctag gcccccgggt cagggctgcc
     5761 tctgcctgag aacctcgcgc tgcctcctga acaggtagcg gatgatgtcc tcgcgcgtat
     5821 cgtcatccac cctgtggaac ctcacggcca cagcccagcg gttcacgtcg ggggtgctcg
     5881 ccaacggctt cagccgcagg acctcgccga tcaggttcac catgaggagg ggcctcatgg
     5941 gcaggatgat gtggagctcc aggatgtcgc cgatatcgta aggctccgct gagatgaacc
     6001 tcatgcccga ggccgatatg ttgacctccc tggggatggt gcggacgagg gccttgaagt
     6061 cttccgagat caccatgttg atgatgaggt cgagcttgcg gtccatctcc cagagatgct
     6121 gctccagcag ggcgcccctt cgtgcaggct gaccggacgg ctcggactcc ccgtaac
//
LOCUS       DAOSCR010000073         6154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8127_length_6154_cov_3.095753, whole genome shotgun sequence.
ACCESSION   DAOSCR010000073 DAOSCR010000000
VERSION     DAOSCR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8127_length_6154_cov_3.095753"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..169
                     /locus_tag="PLT69_03060"
                     /pseudo
     CDS             125..169
                     /locus_tag="PLT69_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            236..571
                     /locus_tag="PLT69_03065"
                     /pseudo
     CDS             236..571
                     /locus_tag="PLT69_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
     gene            complement(602..1183)
                     /locus_tag="PLT69_03070"
     CDS             complement(602..1183)
                     /locus_tag="PLT69_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13455.1"
                     /translation="MDSAVLEFMGTMLLEAAKGQRRIEELSQWFGGGAGGVKGFTEAF
                     ARAYGVEIEACSERSLALWKKTFDALAGSIMELASAMDLVPRRDYAALAEENDALRQR
                     VGELEQEVQRLAALLEGGTRRPADGLEGFSRLIEEQARHYREFMANLGDALGRAEGES
                     GRPFRQEGAGRPPKGASVPGAHRPKKTKKAQPR"
     gene            complement(1176..2768)
                     /locus_tag="PLT69_03075"
     CDS             complement(1176..2768)
                     /locus_tag="PLT69_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HOJ13456.1"
                     /translation="MMSGTDTTRMLFDHYGLFLKGLERFMRIAALSAEASSERAKAAA
                     VLLNGLFKYTSEFMTPFWVSLNAFVATERDKVVRHPLDETVRDYLDLFLFNLQVAAKG
                     FSSSVRAANDHCIEEIRGSYAAFLDTLLDRGGEDIRAFAKRRARLLEKVVYEYPKAIE
                     DIKDEFGFHFDRPGYTLEAETDRFLLYRVHPLPGVNAPERHKPVLIVPPYVLGVNILG
                     FLPGERKSYAHAFANQGIPTYVRVLKDIDTTPAVQTMTGEDDALDTRRFCELIASRHR
                     RPVTLNGFCQGGFIALLDILTGELDGLVDALVTCVAPIDGTRCKALVEYLEHLPQRFR
                     DLGYAVKQLENGNAVVDGKVMSWVYKLKSMEKEAPVYTFFRDLALFDNPSGGETAITK
                     TAAALNHWLVYDRKDIPVAITALSFDSYTVPIAPDGTMPVKLFGKKLNIKDLGEKGIT
                     YLICYASADDLVDRESALAPLDFVQAEVTVFPKGHGAIATTWSAPDSEYAVHKRFPQG
                     MRGPVRFQLDLDEELSGGEDRG"
     gene            complement(2934..3254)
                     /locus_tag="PLT69_03080"
     CDS             complement(2934..3254)
                     /locus_tag="PLT69_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13457.1"
                     /translation="MDSMDFKTLFRQMIDFNKAAFTNTFNAMDLLQDQAEKMMNTFLS
                     QAGFVPKEYRKLVGDWVASCRKARDEFKQAIEEGFERLEGAFADEETAQPQKTAKAQA
                     KSKA"
     gene            complement(3312..3581)
                     /locus_tag="PLT69_03085"
     CDS             complement(3312..3581)
                     /locus_tag="PLT69_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13458.1"
                     /translation="MDYPKDMAKQALQFYKATFDNGYSTMVMLQDQAEKIVRNLMDQA
                     PWMNADARRILDQWIDTYKKGREEFKKAMDDGYAKVEEYFAKVGR"
     gene            4208..4693
                     /locus_tag="PLT69_03090"
     CDS             4208..4693
                     /locus_tag="PLT69_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOJ13459.1"
                     /translation="MPETVTLKKYASRRLYNPKNSSYVTLAQVADMIRSGVDVVVQDA
                     KTGEDVTAFILTQVILEEARNKKALLPPPLLHLIIRSDGSALKEFFDKHLTNAISSYL
                     ALKDAFDEQFNKWLAMGMNYSDLAREAFKTMPMFGPFFGPPGTPGPTAEDRGEEKRRK
                     R"
     gene            5468..5980
                     /locus_tag="PLT69_03095"
     CDS             5468..5980
                     /locus_tag="PLT69_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13460.1"
                     /translation="MPEAGSGTDRVPTVFRSVLALALAVLVWFHGAEAAQYGTAVVTF
                     NVQQISEISFTGSPTLTINSAVAGQEPQPVTDNTARYAISTNGSNMRITAQINKPTPP
                     NTLLEIMLAAPGSGTSAGYVPLSTTPAVVVSGIGPVASGSRVVWYRFSATTQAGVVSD
                     SFTVTLTLSE"
BASE COUNT         1039 a         2143 c         1845 g         1127 t
ORIGIN      
        1 gaagatcgcc atcaccctgc gcggctcact ctcggccagc cacaacacct ggtcgggcat
       61 cctctgggag cagggcgaac tctatacggc gccggtgtat gacatcacgc acatcgtaga
      121 ccgtgtgggt ggaggcgacg cgttcgtcgg cgggctcatc tacggcctgc gcgcctttga
      181 cgaccccggg cgcgccctcc gctttgccgt cgccgcttct tgcctgaagc acagcatcgt
      241 gggcgatttc aaccctgcac atgtcaacga gtcgtacgcg aagaacacct tcttcaagac
      301 acgcatagcc cacgggatgc tcgccgcggg gctggtctcc tcggtgatag gcatgcagct
      361 gccgggaccc gggtccatct acatcagcca ggagctcagg ttcctggcgc cggtccgcat
      421 aggcgacacc gtcaccgcac acgtggaggt caaggacctc aacatcgaga agaaccgggt
      481 gacgctcgcg acgtggtgcg tgaaccagga cggcgtgagg atcctcgagg gagacgccgt
      541 cgcgagcccg ccgaagaagg tcaagaccta gcggggacgc ccggggagga cgcacgccgc
      601 gtcatcgcgg ctgggccttc ttcgtcttct tcggcctgtg agcgccgggg acggacgccc
      661 ccttcggggg ccgcccggca ccctcctgcc ggaaaggcct cccgctctcc ccctcggccc
      721 ggcccagggc gtcgcccaga ttcgccatga actcccggta gtgcctcgcc tgctcctcga
      781 tgagcctgct gaagccctcc aggccgtccg ccggcctccg ggtcccgccc tcgagcaagg
      841 ccgcgaggcg ctgcacctcc tgctcgagct caccgaccct ctgcctgagg gcatcgttct
      901 cctcggcgag tgcggcgtag tccctgcgtg gcacgagatc catcgcggac gcgagctcca
      961 tgatggagcc ggccaaggcg tcgaatgtct tcttccagag agcgaggctg cgttcggagc
     1021 aggcctctat ctcgacgccg tacgccctgg cgaacgcctc ggtgaaaccc ttgacccccc
     1081 cggccccgcc gccgaaccac tgggagagct cctcgatgcg tctctgcccc tttgcggcct
     1141 caaggagcat ggtgcccatg aactcgagaa cggcgctatc cacgatcctc cccccctgac
     1201 agctcctcgt cgagatcgag ctggaacctg acggggccgc gcatgccctg agggaaacgc
     1261 ttgtggacgg catactccga gtccggcgcc gaccaggtcg tcgcgatggc gccgtgcccc
     1321 ttggggaaga ccgtgacctc tgcctggacg aagtccaaag gcgcgagcgc ggactcccgg
     1381 tccaccaggt cgtcggctga ggcgtagcat atgaggtacg tgatcccctt ctcgcccagg
     1441 tccttgatgt tcaacttctt cccgaagagc ttcacgggca tggtcccgtc gggggcgatg
     1501 ggcacggtgt atgaatcgaa gctcaacgcg gtgatggcca cggggatgtc cttgcggtcg
     1561 tagacgagcc aatggttgag tgccgcggcg gtcttcgtga tggcggtctc cccgcccgaa
     1621 gggttgtcga acagggcgag atccctgaag aaggtataga cgggcgcctc cttctccatg
     1681 ctcttgagct tgtacaccca gctcatgacc ttcccgtcaa ccaccgcatt gccgttctcg
     1741 agctgcttga cggcgtaccc caggtccctg aaccgctgcg gaaggtgttc gaggtactcg
     1801 acgagcgcct tgcacctcgt gccgtcgatg ggcgccacgc acgtgacgag tgcgtctacc
     1861 aggccgtcga gctcgccggt gaggatgtcg agaagcgcga tgaacccacc ctggcagaac
     1921 ccgttgaggg tcaccggccg ccggtgcctg gaagcgatca gctcgcagaa gcgcctcgtg
     1981 tcgagcgcat cgtcctcgcc ggtcatggtc tggactgccg gggtcgtgtc tatgtccttg
     2041 agcaccctca cgtacgtggg aatcccctgg ttcgcaaagg catgggcgta gctctttctc
     2101 tctcccggca gaaaccccag gatgttcacg ccgagcacgt acggcggcac gatgaggacg
     2161 ggcttgtgac gctcgggtgc attcacgccg ggcaaggggt gaacgcggta gagcaggaag
     2221 cggtcggtct cggcctcgag ggtgtagccg ggccgatcga agtggaaccc gaactcgtcc
     2281 ttgatgtctt cgatcgcctt cgggtactcg tagacgacct tctcgaggag cctcgcccga
     2341 cgcttcgcaa aagcccggat atcctcccca cccctgtcga ggagcgtgtc gaggaacgcg
     2401 gcgtaagagc ccctgatctc ctcgatgcaa tgatcgttcg cggccctgac gctgctggaa
     2461 aaccccttgg cggccacctg caggttgaag agaaacagat ccaggtagtc gcgcacggtc
     2521 tcgtccaagg ggtgtcgaac gaccttgtcc ctctcggtgg cgacgaacgc gttgagggag
     2581 acccagaagg gtgtcatgaa ctcgctcgtg tacttgaaca acccgttgag gaggacggca
     2641 gccgccttgg ccctctcgct ggaggcctcg gccgacagcg ccgcgatcct catgaaccgt
     2701 tcgagcccct ttaagaacag gccgtagtga tcgaaaagca tcctcgtcgt gtccgtgccg
     2761 ctcatcatcg cttttcctct cgtgtcgtca tctcggccct ctctccacgt atcccccacg
     2821 cggccgtgcg aaggcgatca tcggcgatcg ggcttcgtcc ctcagggtcg gcctcgcccc
     2881 cgttccaggt cgtgtgcggc cctcaggcat gcggtcgacc gaaaacaggc ccctcaggct
     2941 ttcgacttcg cctgggcctt ggccgtcttc tggggctggg cggtttcttc atccgcgaac
     3001 gccccctcca ggcgttcgaa accctcctcg atcgcctgct tgaactcgtc cctggccttc
     3061 ctgcacgagg cgacccagtc gccgacgagc ttcctgtact ccttggggac gaagcccgcc
     3121 tgggaaagga aggtgttcat catcttctcg gcctggtcct ggaggaggtc catcgcgttg
     3181 aaggtgttgg tgaaggcggc cttgttgaag tcgatcatct gcctgaacag cgtcttgaaa
     3241 tccatggaat ccatgcgtca ctctcctcgt tctctcgctc gggcgtcggg ccggatacgc
     3301 gcccaagccc cttacctgcc gaccttggcg aagtattcct ccaccttcgc atacccgtcg
     3361 tccatcgcct tcttgaactc ctccctgccc ttcttgtagg tgtcgatcca ttggtcgagg
     3421 atcctgcgcg catcggcgtt catccagggc gcctggtcca tgaggttcct cacgatcttc
     3481 tccgcctggt cctgcagcat gaccatggtc gagtacccgt tgtcgaacgt cgccttgtag
     3541 aactggagcg cctgcttcgc catgtccttg ggataatcca tgtgtcttac ctccgtttcg
     3601 tgttttcacc gggcgagccc ggtttttctt ttcccgatca gcagggcaag ggctctcacc
     3661 ccatgtcccc cgcctggatc ctctctctcg catcgcagcc gccggctgtg accattcacc
     3721 tgcatccgcc ctggacagcc actcctgaag accgcggccc cgtccacgag acccgcctga
     3781 agcccctccg gtgtagacgc cccacgacgg cctcgcaccc ctcgagggac cgtttcctgc
     3841 atcgcggcaa atcggttccc gggggtgcgg acgacgaccc gcccgccatc tcacggagac
     3901 gacgcgtcac cttgctgcgt ccgcctgcac atcctgggag catgccttac ataataggac
     3961 ctccgggcgc attgtcaaga gagatttttg catcgcagca tcttcgcctc tcgcccgccg
     4021 gcccgacgac ccctttcgca ggcggccctc cccgtggggc ggggcgccgc catttcttgg
     4081 tttcgggcgg gttccacggc ttcagggaac cattcccggc gcgcgggccg tgagggcgac
     4141 cgcccttcga actcgttgtt gcaatgtagc aaactctgtg gtagcaaggt cttcgaggag
     4201 aggttccatg cccgagaccg tcaccctgaa gaaatacgcg agcaggcgcc tgtacaatcc
     4261 caagaacagc tcttacgtga ccctggcaca ggtggccgac atgatacggt cgggcgtgga
     4321 cgtcgtggtc caggatgcga agaccggcga agacgtcacg gcgttcatcc tcacgcaggt
     4381 catcctcgag gaggcgagga acaagaaggc cctcctcccc ccgccgttgc tgcacctcat
     4441 catccggtcc gacgggtccg ccctcaagga gttcttcgac aagcacctca ccaacgccat
     4501 atcttcctac ctcgcgctca aggatgcatt cgacgaacag ttcaacaagt ggctcgccat
     4561 gggcatgaac tactcggacc tggctcgcga ggccttcaag accatgccca tgttcggccc
     4621 cttcttcggc ccaccgggca caccggggcc cacggccgaa gaccgtggcg aggagaaacg
     4681 ccggaagcgc tgaacatgtc ggaaaacatc aggggataca gacccggcct tcccgccgga
     4741 ctcgatctcc tcgtcgctcc atgaatccgg ggacctgaga agcctggggc gatccggcac
     4801 gaatcggccc cttccctgca gacgagcgct ggagagccct gcagcgctct caaggggctc
     4861 ccacccgtgc atccgaacca ggccccacct cccgtgaccc tcggcctccc gcctcgccct
     4921 gggccgcgcc gcaaacaacc ccgccccctc aaggccgccc ttgttcgtgc cggccaggcg
     4981 cccacgccct gacaccccac ctcgtgatcc gcccccacac cttgcgccga ggacgcacag
     5041 cctgaggcct tgaccgagac cgcacacgcg ccacaggcgg ctaggtcatc gaacgcacgg
     5101 cgacaaccag gcgggattca ccgtgtgcgt cccatcggcg gcagggccct ccccttcgcg
     5161 cgtgctgtcc ggtctcccgt cgccgttgga aggcggcatc accctgcacc catgggggtg
     5221 tcttcctcga aggcagcctc acgacggcgt cagccggcct caccctgccg ccatggtgcc
     5281 gccggtatcg tcgaaggccg cccatccacc cgtcaagcat caaggaaaag catttgaagt
     5341 tgactgggcg gccggaatgt acgatatgca caccataaga acgtttcggg gccataggcg
     5401 acgcctcttt cccggaaacg gtgcgcgtgc aggtgcatgg acagtgaaat gatgggggtg
     5461 caagctcatg cctgaggcag ggtccggtac ggacagggtt ccgaccgtgt tccggtccgt
     5521 cttggcgctc gcccttgccg ttcttgtgtg gttccacggg gccgaggcgg cacagtacgg
     5581 cacggcggtg gtcacgttca acgtccagca gatcagcgag atctcgttca ccgggtcgcc
     5641 cacgctcacc atcaacagcg cggtggcggg gcaggagccc cagccggtga cggacaacac
     5701 cgcacgctac gcgatctcca ccaacggatc gaacatgagg atcaccgccc agatcaacaa
     5761 gcccacccca ccgaacaccc tcctggagat catgctcgca gcgcccggct cggggacgag
     5821 cgccggctac gtgccgctgt ccaccacacc ggccgtcgtc gtctcgggca tagggccggt
     5881 tgcgagcggg tcacgcgtcg tctggtaccg cttcagcgcc accacccagg cgggcgtcgt
     5941 gtccgactcg ttcacggtta cgctcaccct ctcggagtga ggccatcggt gaaaggaaac
     6001 cgctcccctc gagcagcccg tgcatccgcg gttacggcct tcgccctcgc gtgctcgctg
     6061 ctggcccctc aagccagcct ggccgagatc tcgatcaccg tctcgggcag ctggacggag
     6121 ttcgtggact atgccgacct cgcgggaggc gccg
//
LOCUS       DAOSCR010000074         6087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8227_length_6087_cov_3.670093, whole genome shotgun sequence.
ACCESSION   DAOSCR010000074 DAOSCR010000000
VERSION     DAOSCR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8227_length_6087_cov_3.670093"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLT69_03100"
     CDS             <1..682
                     /locus_tag="PLT69_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOJ13461.1"
                     /translation="LGHRLVDARLATIERDVLGVRRTDDVPGNEIPSRYLAWLKRRDG
                     ACVSDVFAHNRLDVVSMASLLKHLSGLLASAHTQLRLCEGDVLAAGRLLRDRGLTREA
                     MTLFTRAADSADRCVRREARVSLSLIHKRSGRWAEAARIWEALLDEDPLDVFAACELA
                     KYLEHRVRDHRRAMGVISRVIHAGGEMKPHVRRDLEHRLKRLQEKAHAASQDDDREPD
                     MLQGHSGP"
     gene            725..2074
                     /locus_tag="PLT69_03105"
     CDS             725..2074
                     /locus_tag="PLT69_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOJ13462.1"
                     /translation="MENIVKKIQDLARYHNEVSPELVKAKDIKLGLRNEDGTGVVVGI
                     TSKGSVFGFKRVPREDGSGTMVVPAPGRLLYCGYDAIELARCIQDEGRFGFEEVAYLL
                     LAGELPSKKDLDTFRSFLAKHRGLTKLERGIIMQEAENENQMFALHSVISHLSRCDPN
                     PVSTEIRDVTETCIKLIAKAPTIVAYNYNVSRYRQGAALRIIPPEPDLSTAENFLYLL
                     NGTRPERKDAMLFDLSLMLHAEHGGGNNSTFTVRTVSSSGANTYMAICSGIASLSGYL
                     HGGASEAVIRMMRDIKKNVKDWTSRRQVTSYLKKILDGKAHDGTGKIYGLGHAVYTIS
                     DPRAEILLEKAEELAAEKGMGDELNLMKLVATVGAELIRERKGQIVSPNVDFYSGFVY
                     KMLRIPMELYTPIFAMARMAGWAAHRIEQIIQAKLIRPAYVSSLPKEYTYVPLDERR"
     gene            2139..3227
                     /locus_tag="PLT69_03110"
     CDS             2139..3227
                     /locus_tag="PLT69_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ13463.1"
                     /translation="MPEVPVLLKDVILFGGGLCLLMAIQQLSGRNPDRLDVLGFFLLG
                     ANGIITANVGMALTRDQLEHPLFSLLFIPAIYAVGPLNLFYYHSLLHGRRAIPYKTWL
                     HILPALLALASAAVFMSMGEAYREETLREVFESPGSSPYAMLILAGSVHVLSYLSYLM
                     AVEHGLWDSREIRSEIRFLTVLNLAAIIAVILLTGGFFLEEGVPLAAGGVLLTLIHSA
                     VFLAHHRYPHFFKALSREIKRKRYEKSVLGGVNTELLRARLMELMRDEELYKDMELNL
                     KTVSDLLSITPHQLSQFLNEHLNQDFRNFVNAFRVEEAKRQLVEHPEKGILEICFDAG
                     FNSKSTFNAVFKKHTGKTPREYRAQALT"
     gene            complement(3344..4225)
                     /locus_tag="PLT69_03115"
     CDS             complement(3344..4225)
                     /locus_tag="PLT69_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ13464.1"
                     /translation="MMPPFEIPGTITAQAVKDILRACGVDLVGIARADSVLLSHPPRP
                     PRDLMPTSKSVVVMALAHQLGAVYAPDIMLWTRSKIQTSRLLDEAADLLSRILERKGF
                     LSLAISADKPVEILKRDPRTGRKYRVPKVVGFLSLRHAAVSCGMGEIGRNNLLLTPEF
                     GPHQRLCAVVTEAPLEPDPPWRHSLCSDCGRCIEACPAGALSPSGHDVDRCFMYWTYG
                     FKALPPAAPSQWPSFIRMLLRHMKWRDFLVETGQTLMTDVDNCIECMRACPVGDRWER
                     IRPDRLPPQRTRGMPGP"
     gene            complement(4222..5235)
                     /locus_tag="PLT69_03120"
     CDS             complement(4222..5235)
                     /locus_tag="PLT69_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HOJ13465.1"
                     /translation="MRDRYRRFSYLTDIQGMKTALAEMRGLFFPPQPRLNHEAYRRRT
                     VDPPDEGWFTEAVTNAVTMHAENAMEYPFLGEPVFEKPLVGFACGDDPIFERFKEVIG
                     PFHFTPYEIMRWQALNNGVEPPEPSDVSVVAFVMPLTANTIRDNAACTEWPSERWAQA
                     RLIGEMFSQVMVREIVAALMERGILAVAPDATPFFRKQRYPKTGWASPWSHRHVAYAA
                     GLGTFGMHDFLITEKGSAHRLGSFVVNLRLKPNRPRPEDIHAHCLHYRGIPCLRCALR
                     CPAGAITKDGGHDKEACSKRVARSLAYCNRKYHIFIYGCGLCATGVPCEHEIPLPDKG
                     TGP"
BASE COUNT         1103 a         1871 c         2060 g         1053 t
ORIGIN      
        1 cctcggccac cgtctcgtcg acgcgaggct ggcgaccatt gagagggacg tgctgggggt
       61 caggcgcacg gacgatgtgc caggcaacga gatcccgtcc aggtacctcg catggctcaa
      121 gaggagagac ggcgcctgcg tgtcggacgt gttcgcccac aacaggctcg acgtggtatc
      181 catggcgtcc ctcctcaagc acttgagcgg actccttgcc tccgcacaca cgcagctccg
      241 cctctgcgag ggcgacgtgc tggccgcagg gaggctcctg agggaccgcg ggctcacccg
      301 tgaggccatg accctcttca cgagggctgc tgattctgcg gacaggtgcg tcaggaggga
      361 ggcgcgggtt tccctctccc tgatccacaa gcgttcgggc aggtgggccg aggcggcaag
      421 gatatgggaa gccctgctcg atgaagaccc gctcgacgtg ttcgccgcct gcgagctcgc
      481 caagtacctg gagcacaggg tgagggacca ccggagggcg atgggcgtca taagccgggt
      541 catccatgca ggcggtgaga tgaagcccca cgtcaggcgg gacctggagc acaggctgaa
      601 gaggctccag gaaaaggcgc atgccgcatc ccaagatgac gaccgggaac cagacatgtt
      661 gcaaggtcat tccgggccgt gatagataca tgcacttccg cacatcgagg ttgaaggggg
      721 gagcatggag aacatcgtca agaagatcca ggatctcgca aggtaccaca acgaggtctc
      781 cccggaactg gtcaaggcca aggacatcaa gctcgggctg cgcaacgagg acggcacggg
      841 cgtcgtcgtg ggcatcacgt ccaagggctc ggtgttcggg ttcaagcggg tgccccggga
      901 ggacggctcg ggcaccatgg tcgtccccgc cccggggagg ctcctctact gcgggtacga
      961 cgccatagag ctcgccaggt gcatccagga cgaggggcgg ttcggtttcg aggaggtcgc
     1021 ctacctgctg ctggcagggg aactgccctc gaagaaagac ctggatacct tccgttcttt
     1081 tctcgccaag caccgcggcc tcacgaagct cgagcggggc atcatcatgc aggaggcgga
     1141 gaacgagaac cagatgttcg cgctccactc ggtcatctcg cacctgagca ggtgcgaccc
     1201 caaccccgtg tccacggaga tcagggacgt gaccgagacg tgcatcaagc tcatagcgaa
     1261 ggccccgacg atcgtcgcgt acaactacaa cgtgtcgagg taccggcagg gggcggcgct
     1321 ccgcatcatc cccccggaac ccgatctctc caccgccgag aacttcctgt acctgctcaa
     1381 cggcacaagg ccggaaagga aagatgccat gctgttcgac ctctcgctca tgctgcacgc
     1441 cgagcacggc ggcgggaaca attccacctt cacggtgagg acggtctcca gcagcggcgc
     1501 gaacacgtac atggccatct gctccggcat cgcctcgctc tcgggttacc tccacggcgg
     1561 ggcaagcgag gcggtgatcc gcatgatgag ggacataaag aagaacgtga aggactggac
     1621 gagcagaagg caggtgacct cctacctgaa gaagatcctg gacggcaagg cgcacgacgg
     1681 gacggggaag atctacggcc tcggccacgc ggtctacacg atcagcgacc cgagggcgga
     1741 gatcctcctc gagaaggccg aggagctcgc cgcggagaag ggcatgggtg acgagctgaa
     1801 cctcatgaag ctcgtggcaa ccgtcggggc cgagctcatc agggagcgga aggggcagat
     1861 cgtgtcgccg aacgtggact tctattccgg tttcgtctac aagatgctgc ggatccccat
     1921 ggagctctac acccccatct tcgcgatggc aaggatggcg ggctgggccg cgcacaggat
     1981 cgagcagatc atccaggcca agctgatcag gcccgcatat gtgagctcgc tgcccaagga
     2041 atacacatac gtgcccctgg atgagaggcg gtgaaagatg tcccggcggg tctgtgctgg
     2101 gcatctcgca ggcggctgcg cgggaaggcc caagatgtat gcctgaggtg ccggtgctgc
     2161 tgaaggacgt gatcctgttc ggcgggggcc tgtgtctcct catggcgatc caacagctct
     2221 cgggaaggaa cccggacagg ctcgacgtcc tggggttctt cctgctcggg gccaacggca
     2281 tcataacggc caacgtgggg atggccctga cccgtgacca gctcgagcac ccgttgttct
     2341 ccctgctctt cataccggcg atctacgccg tgggtccgct caacctcttc tactaccact
     2401 ccctgctcca cggccggcga gccatccctt acaagacgtg gctccacatc ctgccggccc
     2461 tgctcgccct ggcatcggcg gcggtcttca tgtccatggg cgaggcatac cgggaggaga
     2521 ccctgcgtga agtcttcgag agtcccggct cgagccccta cgccatgctc atcctcgcgg
     2581 ggagcgtgca cgttctctcg tacctgtcgt acctgatggc cgtggagcat ggcctgtggg
     2641 acagcaggga gatcaggtcc gagatcaggt tcctgacggt tctgaacctg gcggctatca
     2701 tcgcagtgat cctgctcacc ggagggttct tcctggagga gggcgtaccg cttgccgcag
     2761 gcggcgtgct cctgaccctg atccactcgg cggtcttcct ggcccaccac cgctacccgc
     2821 acttcttcaa ggccctttcc agggagatca agaggaagcg ttacgagaag tctgtcctgg
     2881 ggggcgtgaa cacggagctt ttgcgcgcca ggctcatgga gctcatgagg gacgaagagc
     2941 tgtacaagga catggagctc aatctgaaga ccgtctccga cctcctctcc atcaccccgc
     3001 accagctcag ccagttcctc aacgagcacc tcaaccagga tttcaggaac ttcgtgaacg
     3061 ccttcagggt cgaggaggcc aagcggcagt tggtggagca cccggaaaag ggtatcctcg
     3121 agatctgctt cgatgcgggg ttcaactcga agtcgacgtt caacgcggtc ttcaagaagc
     3181 atacgggcaa gacgccgagg gagtaccgcg cgcaggccct gacgtgagga tccccgccct
     3241 gtggatgggg cgggaaggtg tgtccagggc tctttctagg gtcgcgcggg aacgtccgat
     3301 cgtgcggggc aagtcaccgg cccgccggtc tgcgtgaggc ccgtcagggg ccgggcattc
     3361 cccgggtccg ctgcggcggc agcctgtccg ggcgtatccg ttcccaccgg tctccgaccg
     3421 ggcatgcccg catgcactcg atgcagttgt ccacgtcggt catcagggtc tggcccgtct
     3481 cgaccaggaa gtccctccac ttcatgtggc gaaggagcat cctgatgaac gagggccact
     3541 gggagggagc agcaggcggc agggccttga acccgtacgt ccagtacatg aagcaccggt
     3601 ccacgtcgtg ccccgagggg gagagcgccc ctgccgggca cgcctcgatg cacctcccgc
     3661 aatcgctgca cagggaatgc cgccaggggg ggtccggttc gagcggcgcc tcggtcacaa
     3721 cggcgcacag cctctggtgg gggccgaact cgggcgtgag gaggaggttg ttcctgccta
     3781 tctcgcccat gccgcagctc accgccgcat gcctcaggga aaggaacccg accaccttgg
     3841 gcaccctgta cttcctgccg gtgcgagggt ctctcttgag gatctccacc ggcttgtccg
     3901 ccgagatcgc aagggagagg aacccttttc gttccaggat gcgggagagg agatccgcag
     3961 cctcgtccag gagcctggac gtctggatct tgctcctggt ccagagcatg atgtcgggcg
     4021 cgtacacggc cccgagctga tgcgcgaggg ccatgaccac gacgctcttg gatgtgggca
     4081 tgaggtctcg gggcggtctc ggcgggtgtg agagcaagac cgagtccgcc ctggctatgc
     4141 ccacgaggtc caccccgcag gcccgcagga tgtccttgac cgcctgcgcg gtgatcgtcc
     4201 ccggtatctc gaacgggggc atcatggccc ggtccccttg tcggggagcg ggatctcgtg
     4261 ctcgcacggc accccggtgg cacagagccc gcagccgtag atgaagatat ggtacttcct
     4321 gttgcagtac gcgagggaac gggccaccct cttggagcat gcctccttgt cgtggccgcc
     4381 atccttcgtt atggcgcccg ccgggcagcg caaggcgcat ctcagacacg gtatcccgcg
     4441 gtagtggagg cagtgcgcgt gtatgtcctc gggcctgggc ctgttcggct tcagccgcag
     4501 gttcaccacg aagctgccga ggcggtgcgc cgaccccttt tccgtgatga ggaagtcgtg
     4561 catgccgaac gtcccgagcc ccgcggcgta ggcgacatgg cggtgcgacc acggggaggc
     4621 ccaccccgtc ttcgggtagc gttgctttct gaagaacggc gtcgcgtcgg gcgccacggc
     4681 gaggatcccc cgttccatga gggcggccac gatctcgcgc accatgacct ggctgaacat
     4741 ctcgcctatg agcctggcct gcgcccagcg ctcgctcggc cactcggtgc aggccgcgtt
     4801 gtctcgtatg gtgttcgccg tgagcggcat gacaaaggcc accacgctca cgtcgctcgg
     4861 ctcgggcggc tccacgccgt tgttgagggc ctgccaccgc atgatctcgt aaggggtgaa
     4921 gtggaaagga ccgatcacct ccttgaacct ctcgaagatg gggtcgtccc cgcaggcgaa
     4981 ccccacgagg ggcttctcga agacgggctc tccgaggaag gggtattcca tcgcgttttc
     5041 cgcgtgcatc gtgacggcat tggtgactgc ttccgtgaac cacccctcgt ccggcggatc
     5101 gaccgtgcgc ctgcgatagg cctcgtggtt cagccgcggc tggggtggga agaagagccc
     5161 cctcatctcg gcgagggcgg tcttcatgcc ctggatgtcc gtcaggtagg aaaaccgccg
     5221 gtagcggtcc ctcatccttc ttcgcacggc aggtcctctg tcgcccgaag acgatgcggc
     5281 gggggggagg gactcgtcgt tgccgggttt gctgtcatat tgtaaggcat gttacaagta
     5341 tactacaata ccccatgaaa gcggtacctg tttcttcccg ccggcgaagg ccggggtttt
     5401 gggcgtgaaa agtgtatccc gaatcacctt cgaggacgtc caggatgata atcccgggcg
     5461 acaagggccc tttggggggt catcctgggt gcacgcggca ggggtgatgt gaagcccctc
     5521 ctgttcgtgg aggtgcggtg gtggtgtcca tacgggaccg cttgggcgcc ggggcttaag
     5581 ggcctgccct tcgaaggggc cgatcctgaa agaaggatat cgctcgtggg gtttgcaacg
     5641 gcgctgtgtc tcacccgtcc ggccagtgac tgcggtgcgg ggggatggcc acgggggcgc
     5701 tcgctccgaa gggttcgggc gaaaagaacc gaggggaagg tgaacccttc ttcagggcag
     5761 ggggcaggat gggtcgggta gggcccgttt ctgcgaacag gggatcgctc tagagggatg
     5821 gctcgtgccg cagggaggcc cccgggagag cccaggggcg ggtcccagcc gatcgtacgg
     5881 gggctcgggc gcagagatcc tcccgaccct cagagggact ggcatggccg agagtgaagg
     5941 gaaaagcgaa gggaaaaacc gtgagagcgt gatcgtggcc gcggagcccg agtgggtgtt
     6001 cgacctgctc gtggacacga cgaggttcgc cgagttcgtg tgggggtacc gggggaagga
     6061 gagcgggcct gcgaccctgg tcgaggg
//
LOCUS       DAOSCR010000075         6046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8298_length_6046_cov_3.063929, whole genome shotgun sequence.
ACCESSION   DAOSCR010000075 DAOSCR010000000
VERSION     DAOSCR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8298_length_6046_cov_3.063929"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PLT69_03125"
     CDS             complement(<1..1331)
                     /locus_tag="PLT69_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ13466.1"
                     /translation="MCYDDAVQRRIQAMSVPTRAYDNPIETMMNPASIAFVGASNNLT
                     TMGTIQCLNLVTGGYTGEVLPVHPTEPEVLGLKAYPSVDALPKTPELAVLVVPTRLVL
                     ELVEAFGRIGTKRMVIVTAGFRETGDDGRSLEQTLVERARHYGIRFLGPNCLGIVNTS
                     NRLNITVGPVQDFDGALGIASQSGTYVAQVVSYLHRNGVALSKAISVGNEADINIVDC
                     IEYLGADERTKAIGLYIEGIKDVGRFLETASLVSRDKPIVAQYVGGSEAGARSCASHT
                     GAMAGPAHLYEALFEQAGVIQVESIEDVYRIGSALATQPRLKGRRIAVLTNSGGPGTA
                     IANTCDRLGLEVPEFSPGLKERIAELIPGHASAKNPIDLTFHMDMKVLSEEIPSILFE
                     SEEVDGVIIHGIMDTGFLDLLYPAVSRFMNVTKEQLFEAMSIGLDRLVAMPS"
     gene            complement(1451..2068)
                     /locus_tag="PLT69_03130"
     CDS             complement(1451..2068)
                     /locus_tag="PLT69_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13467.1"
                     /translation="MERDRALFEEVISGAIPGFLRVYGWAVPAVTTGYHQKDFPLFDP
                     TLDLPVLRRPTGGGAVLHVDDVTFSLAARAGDVLPARVEAAGLLVTSAVRTALNACGI
                     HATMDGKNRTFSDVCFARSTPLELAVDGSKVMGLALALRRSHVLLQGVIPLRVDRALS
                     RRVFGPQAPEGAKGLLEISGSFDPDRFVELLRENLARALRVPAFA"
     gene            complement(2116..2529)
                     /gene="accB"
                     /locus_tag="PLT69_03135"
     CDS             complement(2116..2529)
                     /gene="accB"
                     /locus_tag="PLT69_03135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOJ13468.1"
                     /translation="MNLKTVKDLIELIKDTDIVEIEWSPERIHIVRRHGVAEPQARQS
                     AENDRSKASIKEKRTVTVTSPLVGTFYRSPSPETPPYVKVGDRVSKNQVLCVIEAMKL
                     MNEIESHVDGIVAAILKQDGERVGYGDQLFIIEEL"
     gene            complement(2530..3093)
                     /gene="efp"
                     /locus_tag="PLT69_03140"
     CDS             complement(2530..3093)
                     /gene="efp"
                     /locus_tag="PLT69_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ13469.1"
                     /translation="MQYSTAQFRKNLKIEIDGEPFTIVDFQHVKPGKGGAFVRTKVKS
                     LLTGRVLDITFRSGEKVDVPDLEEKKMQYLYQDENGYCFMDTETFEQMTLSADQIGDA
                     LGYLKENVEVEVLFHNGSPIGIELPMFMELEVVETDPGLKGNTASGGSKPAKLETGVT
                     IQVPLFISVGEVVKVDTRTGEYIERVS"
     gene            complement(3099..>3846)
                     /locus_tag="PLT69_03145"
     CDS             complement(3099..>3846)
                     /locus_tag="PLT69_03145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOJ13470.1"
                     /translation="RGIEMTPLGGQLKHMRDLKDEREVACIQEAARISEEALEHVLQQ
                     GLVGRTEAEVAFDLEVGMRRRGADAVSFELIVASGPRSAMPHGTASSRVIGKDEVVII
                     DFGCIYRGYCSDQTVTVLTGRPPDGFEEVYRAVDEARLRALKAVTPGMRASDMDRIAR
                     GHLGSRGLDTFFGHGLGHGVGMEVHESPTISSVSEDTLEEGMVFTVEPGVYLPGRFGV
                     RLEDMAVVSDSSCKLLTNIDKGIVRIIDRE"
     assembly_gap    3847..3856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3857..4137)
                     /locus_tag="PLT69_03150"
     CDS             complement(<3857..4137)
                     /locus_tag="PLT69_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOJ13471.1"
                     /translation="MDKRLERARSFLKGLDAILLVSRSNVRYLSGYTGTDAVYVCLQD
                     KDYLFVDARNTLQAKDETKACVVEVSRRWEDIRDVLVEEDVRSLGIESNV"
     gene            complement(4121..4564)
                     /gene="aroQ"
                     /locus_tag="PLT69_03155"
     CDS             complement(4121..4564)
                     /gene="aroQ"
                     /locus_tag="PLT69_03155"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOJ13472.1"
                     /translation="MKVLVMNGPNLNMLGAREPGVYGTTSLERIEEALAKKAAGLGIE
                     LEFFQSNHEGALVDKVQAAPGQGVDALILNPGGYTHTSVALRDALLAVNLPFIEVHVS
                     RVASREQFRQVNYFSDIARGAITGLGPLVYELALEALWRLHGQEA"
     gene            complement(4561..5097)
                     /locus_tag="PLT69_03160"
     CDS             complement(4561..5097)
                     /locus_tag="PLT69_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ13473.1"
                     /translation="MAGSSEKVQKAFDEGRYLDVIKAARTRKNPEDKLLAGISLYRLS
                     RFAEARDVLEKVSALTKSLATAEYYLALIHGKEGDDDAYRACLERYLAYHPDDDEARD
                     LLEGDTMLRREEFVTEPSVELARIYAQQGHYEQALDIYSRLAESRGLDEDARRHASAV
                     QNLYIMKTLEEWLVRLRK"
     gene            complement(5060..>6046)
                     /gene="aroB"
                     /locus_tag="PLT69_03165"
     CDS             complement(5060..>6046)
                     /gene="aroB"
                     /locus_tag="PLT69_03165"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOJ13474.1"
                     /translation="GRHVKPEGFFAVVDSRVMDLHGPFLRSCLGKIPCTFMTVPPGEG
                     TKSLEVLGQVLETMADAGVNRQWACIAIGGGVTGDLAAFASSVYMRGIPVIHVPTTLL
                     AQVDSSIGGKTAVDLAQGKNLAGTFHQPVLVIADTEFLETLDEDLVRDAMAEVVKYAI
                     TLDAPLFHYLEEAHELDYARVVRMCALDKASVVARDEREGGLRRVLNFGHTLGHAVEH
                     ALGYSTSHGKAVATGMHFAVWLSQRLELIDEKTAIRMRRLIQKWTYDPRTLYCPEPGL
                     VESAMASDKKSTLDDIHFVLTCGIGGATVKKLSRSQILGAYGRFVGESAEGL"
BASE COUNT          976 a         2032 c         1876 g         1152 t
ORIGIN      
        1 gaaggcatgg ccacgagcct gtcgagcccg atgctcatcg cctcgaagag ctgctccttg
       61 gtcacgttca tgaacctact gaccgccggg tacaggagat ccaagaaccc cgtgtccatg
      121 atgccgtgga tgatgacgcc gtcgacctct tccgactcga agaggatgct cgggatctcc
      181 tcagagagca ccttcatgtc catgtggaac gtgaggtcga tggggttttt cgcgctcgcg
      241 tggccgggga tgagctcggc gatccgctcc ttgagcccgg gcgaaaactc gggcacctcg
      301 agaccgagcc tgtcgcacgt gttcgcgatg gccgtgcccg ggccgccgga gttggtgagc
      361 accgcgatgc gcctgccctt cagccggggc tgggtcgcga gcgcggagcc gatcctgtag
      421 acgtcctcta tgctctccac ctggatcacg ccggcctgct cgaacagggc ctcgtagagg
      481 tgcgccggcc ccgccatggc gccggtgtgg ctcgcgcacg agcgggcccc cgcctcggac
      541 cctcccacgt actgcgcgac gatgggcttg tcgcggctca ccaaggacgc ggtctcgagg
      601 aacctcccca cgtccttgat cccctcgatg tagaggccta tcgccttcgt ccgctcgtcc
      661 gcacccaggt actcgatgca gtcgacgatg ttgatgtccg cctcgttccc gacgctgatc
      721 gccttgctca acgccacgcc gttcctgtgg aggtacgaga ccacctgggc cacgtacgtc
      781 ccgctctggg aggcgatgcc gagcgcgccg tcgaagtcct ggacagggcc caccgtgatg
      841 ttcagcctgt tcgaggtgtt cacgatgccg aggcagttcg gcccgaggaa cctgatcccg
      901 tagtgcctcg cgcgctccac gagggtctgc tcgagcgacc ggccgtcgtc acccgtctcg
      961 cggaaacccg ccgtcacgat gaccatccgc ttcgtgccga tcctcccgaa ggcctccacg
     1021 agctcgagga cgagcctggt gggaacgacg agcacggcga gctccggcgt ctttgggaga
     1081 gcgtccacgc tcgggtacgc cttgagcccc aacacctcgg gctcggtcgg gtggaccggc
     1141 agcacctctc ccgtgtaccc gcccgtgacg aggttcaggc actggatcgt gcccatggtg
     1201 gtcaggttgt tgctcgcgcc cacgaaggcg atcgatgccg ggttcatcat cgtctcgatg
     1261 gggttgtcgt atgcacgggt gggaacgctc atcgcctgga tcctcctctg aacggcgtca
     1321 tcgtagcaca tggcgccggc tcgtcccggg ggcaccccct cggtcgttca ccggtctccc
     1381 cccaaaagta tagaacataa catatgggaa atccatcatg aagagaacct gtttctcctg
     1441 cgtgtgcacg ttaggcgaag gcagggacac gaagcgcacg ggcgaggttc tccctcagga
     1501 gctcgacgaa gcggtccggg tcgaaggagc ccgatatctc gaggagccct ttcgccccct
     1561 cgggtgcctg ggggccgaac acccggcggc tcagcgccct gtccaccctg aggggtatca
     1621 ccccctggag gaggacatgg gatctccgca gagcgagggc gagccccatg accttggaac
     1681 catcgaccgc gagctccaag ggcgtggacc gtgcgaagca cacgtccgag aacgtgcggt
     1741 tctttccgtc catggtcgcg tggatcccgc acgcattgag cgccgttcgg acggccgatg
     1801 tcacgagcag gccggccgcc tccacgcggg cgggcaggac atcccccgcc ctggctgcga
     1861 gggaaaacgt gacgtcgtcc acgtgcagca ccgccccgcc tcccgtgggc cgcctcaaca
     1921 cgggcaggtc gagcgttggg tcgaacaggg ggaagtcctt ctggtggtac ccggtggtca
     1981 ccgcagggac agcccagccg tacaccctca ggaaacccgg tatggccccg gagatgacct
     2041 cctcgaagag ggcacggtcc ctctccatgg tcgcttgggg ggggacgcct ccttcacgga
     2101 agaatggcca cggtctcata gctcttcgat gatgaagagc tggtctccgt accccaccct
     2161 ctcgccgtcc tgcttgagga tcgcggccac gatgccgtcc acgtgggact cgatctcgtt
     2221 catgagcttc atggcctcga tgacgcacag gacctggttc ttggacaccc tgtcgcccac
     2281 cttcacgtaa gggggtgtct ccggggacgg cgagcggtag aaggtcccca cgaggggcga
     2341 ggtcacggtg accgtgcgtt tctcctttat gctcgccttc gagcggtcgt tctcggccga
     2401 ctgcctggcc tggggctctg ccacgccatg gcgccggacg atgtggatcc tctcggggct
     2461 ccattcgatc tcgacgatgt cggtgtcctt gatgagctcg atgagatcct tgacggtctt
     2521 caggttcatt cagctcaccc gctcgatgta ctcgccggtg cgcgtgtcga ccttgaccac
     2581 ctcgcccacg ctgatgaaga gcggcacctg gatcgtgacc cccgtctcca gcttggccgg
     2641 ctttgaaccg ccggacgccg tgttgccctt gaggccggga tcggtctcga ccacctcgag
     2701 ttccatgaac atggggagct cgatgccgat gggggacccg ttgtggaaga ggacctccac
     2761 ctccacgttc tccttgaggt agccgagtgc gtcgccgatc tggtcggcgc tcagcgtcat
     2821 ctgttcgaag gtctccgtgt ccatgaagca gtacccgttc tcgtcctggt agaggtactg
     2881 catcttcttt tcctcgaggt ccgggacgtc gaccttctcc cccgacctga acgtgatgtc
     2941 caggaccctg ccagtgagga ggctcttcac cttcgtcctg acgaaggccc ctcccttgcc
     3001 gggcttgacg tgctggaagt ccacgatggt gaagggctcg ccgtcgatct cgatcttgag
     3061 gtttttcctg aactgtgctg tggaatactg catcgagctc actcccggtc gattattctg
     3121 acgatcccct tatcaatatt cgtgaggagc ttgcaagagc tatctgagac caccgccatg
     3181 tcctcgagcc tcacgccgaa cctgcccggc aggtacacgc cgggctcgac ggtgaagacc
     3241 atgccttcct cgagggtgtc ctcggaaacc gacgagatcg tcggcgactc gtggacctcc
     3301 atgcccacgc cgtggccaag gccgtggccg aagaaggtat cgagcccgcg cgagccgagg
     3361 tggcccctgg cgatacggtc catgtccgag gccctcatcc cgggggtgac ggccttcagg
     3421 gcgcgaagcc gcgcctcgtc gaccgcccgg tacacctcct cgaagccgtc cggcggcctg
     3481 ccggtgagca cggtcacggt ctggtccgag cagtagccgc ggtagatgca cccgaaatcg
     3541 atgatgacca cctcgtcctt gcctatgaca cgggacgacg cggtgccgtg gggcatcgcg
     3601 gaacgggggc ccgaggccac gatcagttcg aacgagacgg catccgcccc cctccttcgc
     3661 atgcccacct cgagatcgaa ggccacctcc gcctccgtcc tcccgacgag cccctgctgg
     3721 agaacgtgct cgagcgcctc ctcggaaatc ctcgccgcct cctgtatgca ggccacctcg
     3781 cgctcgtcct tcaggtccct catgtgcttg agctgccccc cgagcggcgt catctctatg
     3841 ccgcggnnnn nnnnnnacgt tggactcgat gccgagcgac ctgacgtcct cctccacgag
     3901 cacgtcccgg atgtcttccc agcgcctcga gacctcaacc acgcaggcct tcgtctcgtc
     3961 tttggcctgg agggtgttcc tggcgtccac gaagaggtag tccttgtcct gcaggcagac
     4021 atagaccgcg tcagtccccg tgtaaccgct caagtacctg acgttggaac gggagacgag
     4081 caggatggcg tcaagaccct tgagaaagga ccgggcgcgc tcaagcctct tgtccatgga
     4141 gtctccacag cgcctcgagg gcgagctcgt ataccagcgg gccgagcccg gtgatggcgc
     4201 cccgggcgat gtcggagaag tagttcacct gcctgaactg ctcgcgcgag gcgaccctcg
     4261 agacgtgcac ctcgatgaag ggcaggttca ccgcgagcag cgcgtccctg agggcgaccg
     4321 aggtgtgggt gtagcctccc gggttcagga tgagcgcgtc gacgccctgg ccgggggcgg
     4381 cctggacctt gtccacgagc gcgccctcgt ggttcgactg gaagaactcc agctctatcc
     4441 cgaggccggc ggccttcttc gcgagagcct cctcgatccg ctcgaggctc gtcgtgccgt
     4501 agacacccgg ttccctggcg cccagcatgt tgagattcgg gccgttcatg acgaggacct
     4561 tcatttgcgc aacctcacga gccattcctc caaggtcttc atgatataga gattctggac
     4621 ggcgctcgcg tgcctcctgg catcctcgtc cagcccccgg ctctcggcga gcctggaata
     4681 gatgtcgagc gcctgctcgt agtgcccctg ctgggcatag attctcgcca gctccacgga
     4741 gggctcggtg acgaattcct cgcgcctgag catggtgtcg ccctcgagga ggtccctggc
     4801 ctcgtcgtcg tccgggtggt aggcgaggta acgttcgagg caggccctgt acgcgtcgtc
     4861 gtcgccctcc ttgccgtgga tcaacgccag gtagtactca gccgtggcga ggctcttggt
     4921 gagggcggac accttctcga gcacgtccct cgcctcggcg aaccgcgaca gcctgtagag
     4981 ggagatcccc gccagcagct tgtcttcggg gttcttcctc gtcctggcgg cctttatgac
     5041 gtccaggtac ctgccttcgt caaaggcctt ctgcactttc tccgacgaac cggccatacg
     5101 cgcctaatat ttgagaacga gagagttttt ttaccgtagc accgccgatg ccgcacgtca
     5161 acacaaagtg gatgtcgtcg agggtggatt tcttgtccga tgccatggcg ctttccacga
     5221 ggcccggttc tgggcagtag agcgtacgcg ggtcgtaggt ccacttctgg atcagccgcc
     5281 tcatcctgat ggcggtcttc tcgtcgatga gttccagcct ctgcgaaagc cacacggcga
     5341 agtgcatccc ggtcgcgacc gccttgccgt ggctcgtgct gtatcccagg gcgtgctcca
     5401 cggcgtggcc cagggtgtgc ccgaagttga gcaccctcct caagccgccc tccctctcgt
     5461 ccctcgccac cacggacgcc ttgtcgaggg cgcacatcct caccacgcgt gcatagtcca
     5521 gttcgtgggc ctcctccagg tagtggaaga ggggcgcatc cagggtgatc gcgtacttga
     5581 cgacctccgc catggcgtcc ctcacgaggt cctcatcgag ggtctcgagg aactccgtgt
     5641 ccgcgatcac caagacgggt tggtggaagg tgcctgcaag gttcttgccc tgcgcgaggt
     5701 cgaccgccgt cttcccgcct atgctcgagt ccacctgggc caggagggtg gtgggcacgt
     5761 ggatcacggg gatgccccgc atgtagacgc tggaagcgaa cgcggcgagg tcgccggtca
     5821 ccccgccgcc tatggcgatg catgcccact ggcggttcac cccggcatcc gccatggtct
     5881 cgagcacctg gccgagcacc tccagggact tcgtcccctc gccgggcggc acggtcatga
     5941 aggtgcaggg gatctttccc aggcacgacc tcaaaaacgg cccgtggaga tccatgaccc
     6001 gggagtccac cacggcgaag aacccctcgg gcttgacgtg ccttcc
//
LOCUS       DAOSCR010000076         6029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8327_length_6029_cov_3.670070, whole genome shotgun sequence.
ACCESSION   DAOSCR010000076 DAOSCR010000000
VERSION     DAOSCR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8327_length_6029_cov_3.670070"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..444
                     /locus_tag="PLT69_03170"
     CDS             148..444
                     /locus_tag="PLT69_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13475.1"
                     /translation="MIKGTDTPQACSVCGTHEAVDSCRECGASLCRKCRVFEVGRAGK
                     DEVEVVAFCPACFERLANHRKESLNGVFGLRRITDLVNQDRQQKIARFKIKLTS"
     gene            complement(1256..2014)
                     /locus_tag="PLT69_03175"
     CDS             complement(1256..2014)
                     /locus_tag="PLT69_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ13476.1"
                     /translation="MGSRYPSSDFENYTRRIRDYPLLSPEEEIACARRYKEGDIESGK
                     RIITANLRFVVKISQPYFKLGYSPLEIVQEGNMGLVKALTRFNPDMGVRFICYAIWWI
                     KAYIKNFINKSYQVHTGRLTHAKGLVSLDSALPGDGNCEDCLLDHLLYEGPDQDDFYS
                     HKERHRYLLGLLHCDPPILSEREALIIEKRFFSDPPLTLKDIAGIIGVTRERVRQIEV
                     RSLQRMREAIHREHSILVEDLHIGHEYPMRRKKI"
     gene            complement(2175..2810)
                     /locus_tag="PLT69_03180"
     CDS             complement(2175..2810)
                     /locus_tag="PLT69_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOJ13477.1"
                     /translation="MEDFVYWLVETIGRLGYPGVLVLMFLESTFFPVPSEIVIPPAGY
                     LAARGEMNLVLVIMCGVAGSILGALFNYWLAVLLGRPLILRYGKYFLLPPKRFERVNA
                     FFTAHGEISTFTGRLVPGLRHFISFPAGLARMKMTTFLGYTALGSAVWVTILAYIGKV
                     VGDNMDLVKKYSHQALLITLAVMALVFVVYVMQYRKRSLIKILDDKARRTE"
     gene            2968..4260
                     /locus_tag="PLT69_03185"
     CDS             2968..4260
                     /locus_tag="PLT69_03185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOJ13478.1"
                     /translation="MHTPSEDLLLHVECMTYGGRGLARRPDGKVVFVDGALPGETVMA
                     RVTSDRRDFAEACVVQVMERSPLRTEPFCESFGLCGGCDWQHALYRHQVEFKRGILED
                     ALTRAVKTPFETEPDVPSPQDRNYRCRATLRVVLGASGGLGFFARRSHEVVRIRSCPL
                     LSDRVREIVASLERELGAVTQGRAVSVDVAAPSRQALVAVTIDGAPEKAAISRADNLR
                     RALGIEGLSLRFTQSKKTWIIGSPHAWYSLETGRERFTMAFGIGGFVQANPPVNTLLV
                     EDVVAKVSGSREVLDLFSGSGNFAVPLGPHASSVVAVEHDETLVRAGRAASSENKCSN
                     VTFVRGDALRTLTRLKREGRRFDAVILDPPRKGARDIAEVLDPGVAPLVIYVSCNPST
                     LARDVSLLTRRGFRLESTRLYDMFPHTHHIESLTVLKS"
     gene            4312..5157
                     /locus_tag="PLT69_03190"
     CDS             4312..5157
                     /locus_tag="PLT69_03190"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOJ13479.1"
                     /translation="MGIVCKEDNPEALSLSSEVIRRLSERGVKCLYEDHVASSCNLAP
                     TGRVWSDSDLLVVIGGDGTILRAIRMSLPREVPILGVHMGYLGFLTEVTNEEVVQALD
                     ELLEGRYTIERRTMLEVTLDRSGTILAHHLVINDMVINKGALARIIHIEVLTDEVFVT
                     SIWGDGLIVATPTGSTAYNLASGGPIVHPDVCALVLTPICPHVLSNRSIVLPDTQEIV
                     IAVKSGKASDNIYLTLDGQIGHSLVAEDRIRVRRASTGASMVRLGGRRNYFEVLRSKL
                     KWQER"
     gene            5154..>6029
                     /locus_tag="PLT69_03195"
     CDS             5154..>6029
                     /locus_tag="PLT69_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13480.1"
                     /translation="MIEVLRISSLAIFDEVELELEEGLNCITGETGAGKSLVLNALTL
                     LMGARAGRDLVRPGRDRCAVEALFATEDGETVLRREVTASGQSRCYIDGRLATLAQLA
                     GKASELVHIYGQHDYQDLLLPRQQMAILEETAGLSRLEAETAYREYRDALESLMELRE
                     RIRSASEDRSYLEHCLAEIRSAGIEEGLEESLERELHLARKAASLRESAQAAEELLYS
                     GSPSLADMASRVRQILQRISKDDPLMEDLAERMGDIGAALADAYELLRTRIERYEADP
                     QRIDDIAQRLDRLRSL"
BASE COUNT         1213 a         1809 c         1897 g         1110 t
ORIGIN      
        1 aacgcatgga caccccgctg cccaccggtt catgctagag accgggccgc agtccaacct
       61 cttgatatca tgggaaccat gtcgctgtcc cggtctggca tggcattcgc ttatgcccct
      121 cctgatgagc gaagagggga ggcatcgatg atcaagggaa ccgatacgcc ccaggcctgc
      181 agcgtgtgcg ggacgcacga agccgtggat tcctgcaggg agtgcggggc ttccctgtgc
      241 aggaagtgcc gggtctttga ggttggaagg gcagggaagg acgaggtgga ggtggtggcc
      301 ttctgcccgg cgtgcttcga acgtctggcg aatcacagga aagagagcct aaacggcgtg
      361 ttcggcctga gacgcatcac cgacctcgtg aaccaggaca ggcagcagaa gatcgcgaga
      421 ttcaagataa agctcacctc atgaacgggg gtcttccatg agatatggca tcctgaccat
      481 aagctgggta cggcatcttt ggtgtctcca ccatatgacc tgtaccctga gcaatattcg
      541 atgacaaatc aataagatac aagatcgcca cgcctggcat ggttttggca aaaaagtgtt
      601 tcagccggga acaaagatcc aggctggacg gctcccccaa gatggggaaa tgggttgaga
      661 tatgtgaggc aagatgccgc aggggtcccc gggaccctca aggggtacaa gggaagatac
      721 agcacaaggg gctgagaaca agagggaagg acaagaaaaa ggcaatactc aagacaagac
      781 cgtaactgaa acctcctttt aacgccagaa gaagggacac accatccgca accgtcccca
      841 cacctgattc ttctggcttt tttttgccct caggcagccg tcaccggcca tggtcgtcct
      901 tgccgccccg tgcgtgggaa tcccgccatg acctggtacg gccgcatcga gcggcctcgt
      961 tccgggagga aggtgatctt ccctccttgc ggcggcagcc aggtccttgt acccaccccc
     1021 atggcgcaag gctgtatctc ggcccggcca ctggttctca cggcatcaga tcccaccacc
     1081 aaggcgcata gctgcatgtc cggggtttcg gcgtcagcat ggacatgggg tacgtacacc
     1141 caccccccgg gcgcaaagct gcacggtcat ggagggcttc gacgcccggg catggggcat
     1201 ccgcgtgcgc ccgatggtcg ctccaccctg gacgtggggc cgtctcgggt gagtgctaga
     1261 tcttcttcct cctcatggga tactcgtggc cgatgtggag gtcttcgacg aggatggaat
     1321 gttcccggtg tatggcctcg cgcatccgct ggagggagcg gacctctatc tgtctgaccc
     1381 gctctctggt cacgcctatg atgccggcga tatccttgag ggtgagaggc gggtcgctga
     1441 agaagcgctt ctcgatgatg agggcttcgc gctcgctcag gatgggcggg tcgcagtgca
     1501 gcaggcccag gaggtagcgg tgcctttcct tgtgggagta gaagtcgtcc tggtcggggc
     1561 cctcgtagag caggtggtcg aggaggcagt cctcgcagtt gccgtcaccg ggcagggcgc
     1621 tgtccagaga cacgagccct tttgcatgcg tgagcctgcc ggtatggacc tggtagctct
     1681 tgttgatgaa gttcttgatg taggccttga tccaccagat ggcatagcag atgaaccgca
     1741 cccccatgtc cgggttgaaa cgggtgaggg ccttcaccag gcccatgttc ccctcctgta
     1801 cgatctcgag cggtgagtac ccgagcttga agtacggttg agagatcttc accacgaacc
     1861 tgaggttcgc cgtgatgatc cttttgcccg actcgatatc gccttctttg tacctccggg
     1921 cgcaggcgat ctcttcctcg ggggaaagga ggggatagtc cctgatccga cgggtgtagt
     1981 tctcgaaatc agaagaagga taacggctcc ccatagaccc tccagaaaag gagatgatca
     2041 aatgtgcgca ctaactttca catttgtcaa gcccaaaaac aacatgggtg ccatacgata
     2101 ttatttgtat gaaatcagat cattgaacca cgttcaatgg aacgccttga aattatattg
     2161 cacacattga ggattcattc agttctccgc gccttgtcat ccaagatctt gataagagat
     2221 ctcttcctgt actgcatgac atacacgacg aacacgagcg ccatgacggc gagcgttatg
     2281 aggagggcct ggtgggaata cttcttcacg agatccatgt tgtcgcccac gaccttgccg
     2341 atgtaggcga ggatcgtgac ccacactgcg gacccgagtg cggtgtatcc caagaacgtc
     2401 gtcatcttca tccgggcgag gcctgcaggg aacgatatga agtgacggag tccgggcacg
     2461 agccttcccg tgaacgtact gatctccccg tgcgccgtga agaaggcgtt caccctctcg
     2521 aagcgcttgg gtgggagcag gaagtacttg ccgtagcgca ggatgagggg cctgcccaag
     2581 aggacggcca gccagtagtt gaacagggcg cccaggatgc tccccgccac cccgcacatg
     2641 atcaccagca cgaggttcat ctctcctcgg gcggccaggt acccggcagg agggatgacg
     2701 atctcgctgg gcacagggaa gaaggtgctc tcgaggaaca tgagcacgag cacgccgggg
     2761 tagcccagcc tgcctatcgt ctccacgagc cagtatacga agtcttccat cttcgagatt
     2821 gtaaaggaac gcgtcttgga ttgcaatctc cggccgacag ggttacatga ctctcccagg
     2881 agacgggatg tcgagagaga ttccccatcg agggtgcagc ttgagtgaca tccgccacgg
     2941 tcaaggcacg gcgaggctct cgtgatcatg cataccccaa gcgaggatct tctcctccac
     3001 gtcgagtgca tgacctacgg cggcaggggg ctcgcgagac gccctgacgg gaaggtcgtc
     3061 ttcgttgacg gcgccttgcc cggggagacc gtgatggccc gggtgacgag cgacaggagg
     3121 gatttcgcgg aggcgtgcgt cgtccaggtc atggagagat cccccctcag gaccgagcct
     3181 ttctgcgaat cgttcggcct ctgcggcggg tgcgactggc agcatgccct gtaccgacac
     3241 caggtggagt tcaagcgcgg catcctcgag gacgcgttga cgagggctgt caagaccccg
     3301 ttcgaaacag aaccggacgt cccctcaccc caggacagga actacaggtg cagggcgacc
     3361 ctcagggtcg tcctgggagc gagcggcggt ctcgggttct ttgcaagacg ttcgcacgag
     3421 gtggtccgca tcaggagctg ccccctcctg tccgacaggg tacgggagat cgtcgcttcc
     3481 ctcgagaggg agcttggggc cgtaacgcaa ggccgtgccg tgagcgtgga tgtcgccgca
     3541 ccttcccgac aggccctggt ggctgtcacc atcgacggcg caccggaaaa ggcggcgatc
     3601 tcacgtgcag acaacctcag gcgtgccctc gggatcgagg ggctcagcct caggttcacg
     3661 caatcgaaaa agacctggat catcgggagt ccccacgcat ggtacagcct ggagacgggc
     3721 cgcgaacgct tcacgatggc tttcggcatc ggcgggttcg tccaggcgaa cccacccgtg
     3781 aacaccctgc tggtcgagga cgtcgtcgcg aaggtctccg gttctcgcga ggtgctggat
     3841 ctgttttccg ggtccggcaa cttcgccgtc ccgctcgggc cccacgcatc gagcgtggtc
     3901 gccgtcgaac acgacgagac gctcgtacgg gcggggaggg cggcctcgag cgaaaacaag
     3961 tgcagcaacg tgacgttcgt ccgcggggac gccctgcgta cgctgacacg cctgaagcgg
     4021 gaaggccgca ggttcgatgc ggtgattctc gatcccccac gcaagggcgc acgggacata
     4081 gccgaggtcc tcgacccggg tgtcgccccc ctcgtcatct atgtctcctg caacccgtcg
     4141 accctggcca gggacgtctc cctgctcacg aggagagggt tcaggctgga atccacgagg
     4201 ctctacgaca tgttcccaca cacgcatcac atcgagtccc tcacggttct caagtcctga
     4261 gtttcgtctg gacaatcttc gggatacgcg ggtatatcaa gcacgatggt ggtgggtatc
     4321 gtctgcaagg aggacaatcc cgaggccctc tccctttcct cggaggtgat acgccgtctg
     4381 tccgaacgcg gcgtgaagtg cctctacgaa gaccacgtcg cctcttcttg caatctggcg
     4441 ccgaccgggc gcgtctggtc ggactccgac cttctcgtgg tgatcggggg ggacggcacg
     4501 atcctcaggg cgatccgcat gtccctgccc cgggaggtcc ccatcctcgg cgtacacatg
     4561 ggctacctcg gtttcctcac cgaggtgacg aacgaggagg tcgtgcaagc cctcgatgag
     4621 ctcctcgaag gcagatacac catcgagagg aggaccatgc tggaggtgac gctggatcgc
     4681 tcagggacga tcctggcaca ccatctcgtc ataaacgaca tggtcatcaa caagggggcc
     4741 ctggccagga tcattcacat agaggtgctc accgacgagg tcttcgtcac gagcatctgg
     4801 ggtgacggcc tgatcgtggc cacgcccacc ggctccaccg cgtacaacct ggcatcgggc
     4861 ggccccatcg tccacccgga cgtctgcgcc ctggtgctga cgcccatctg cccgcatgtg
     4921 ctgtccaaca ggagcatcgt cctgcccgac acccaggaga tcgtgatcgc ggtgaagtcg
     4981 ggcaaggcgt ccgacaatat ctacctcacg ctcgacgggc agatcggcca ctccctcgtc
     5041 gccgaggaca ggataagggt gaggagggcc tcgacggggg cgtccatggt ccgcctcgga
     5101 gggcggagaa actacttcga ggtccttcgc agcaaactca agtggcagga gcgatgatcg
     5161 aggttctcag gatctcttct ctcgcgatct tcgacgaggt ggagctcgag ctcgaagaag
     5221 ggctcaactg catcaccggc gagaccgggg ccgggaaatc cctcgtcctg aacgcgttga
     5281 cccttctcat gggcgcccgt gcgggacggg acctcgtccg cccgggtcgc gacaggtgcg
     5341 cggtcgaggc gctctttgcg accgaggacg gcgagacggt gctcaggcgc gaggtgaccg
     5401 cctccggcca gagcaggtgc tacatcgacg gcaggctcgc gacgctggcc cagctcgccg
     5461 ggaaggcatc tgagctcgtc cacatatacg gccagcacga ctaccaggac ctcctgcttc
     5521 ccaggcagca gatggccatc ctcgaggaga cggccggcct gtcgaggctg gaggccgaaa
     5581 ccgcgtaccg tgagtacagg gatgcgctgg agtccctgat ggaactgagg gaaaggatca
     5641 ggtccgcctc ggaagaccgt tcgtaccttg aacactgcct ggcggagatc cgttcggctg
     5701 gcatcgagga ggggctcgag gagtctctgg agcgcgagct gcacctcgca aggaaggcgg
     5761 cttccctcag ggagtcggcc caggctgcgg aagaactcct ttacagcggc agcccttctc
     5821 tcgcggacat ggcatccagg gtgcgccaga tccttcagcg catctcgaag gacgaccctc
     5881 tcatggagga cctggccgag cgcatgggtg acataggcgc cgccctcgcg gacgcctatg
     5941 aactcctgcg cacgaggatc gagagatacg aggccgaccc gcaacgcatc gacgacatcg
     6001 cccaacggct ggacaggctc aggtcgctg
//
LOCUS       DAOSCR010000077         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8420_length_5980_cov_3.529283, whole genome shotgun sequence.
ACCESSION   DAOSCR010000077 DAOSCR010000000
VERSION     DAOSCR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8420_length_5980_cov_3.529283"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..980
                     /locus_tag="PLT69_03200"
     CDS             417..980
                     /locus_tag="PLT69_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004725823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOJ13481.1"
                     /translation="MDIESIIIQYGYIFILIGTFFEGETVLVLAGFLVHQGYLSFTPV
                     VAAAFIGTYVGDQLYFYIGRRKGIDLLDRNPSWKRQSGRVFPLLQHYQTMVIIGFRFI
                     YGIRTVTPFILGASGVSPRRFMVLNAVGGIAWSLGIASLGYMLGYTAEVIIQDIKRYE
                     YIIMASIVMAGIVFWGIYLIRRKRRGV"
     gene            1064..1765
                     /locus_tag="PLT69_03205"
                     /pseudo
     CDS             1064..1765
                     /locus_tag="PLT69_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
     gene            complement(1959..2234)
                     /locus_tag="PLT69_03210"
     CDS             complement(1959..2234)
                     /locus_tag="PLT69_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ13482.1"
                     /translation="MEETFRGCRACGKWRPARGEDGLLRLMEYGVQMVLEEELTAYLK
                     AGSQENTGQRAGYRNRYKPRVLMTRLGRMELMSSRGHFILSPDNKGK"
     gene            3124..4311
                     /locus_tag="PLT69_03215"
     CDS             3124..4311
                     /locus_tag="PLT69_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ13483.1"
                     /translation="MHPVERVLRTFEGKETDRVPAFCAMVEGRTYNEVLGKPLVSQER
                     LLVNPVATFALDRWGPLLTKLVFQHEVTRSMEKRIKAQVELGFDAVWAILDETFIVTD
                     HRTMVRFTGSRYNLQPDGYGNMTYMYREPCFKTREDFLEWPYWPDADDVAHSAYRFFS
                     AMMKKYGHRACLFGQASAYGIQESLLWAVGFERLPLWIRRERDLVKRFIEINEELCLK
                     TNMALLDAGVKVVIQTDDFAFKSGPIMNPRLIDELFGPSYRRIIKAVHDRNGKYVLHS
                     CGDNTLLFDTFISWGVDGLHAYETTSNVDIFKEKELHGDKVTIIGGVGVDYLLTDRSR
                     DEEVVREVRRLIARLGPSGRFIISPVHSLSTIPAHKLRVMVDAVRTYGRYPLSPALEE
                     DAG"
     gene            4308..4949
                     /locus_tag="PLT69_03220"
     CDS             4308..4949
                     /locus_tag="PLT69_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOJ13484.1"
                     /translation="MNDSLRLVCEAVDTLDIDGIRDLVQRCLEEGHTPEEIIEHGIGK
                     GLDLVGKKFEAGEYFLADLVMAGEVVKEVMPVLKERMNPVDAPRRQKVILATVEGDIH
                     EIGKNIVGIILSANGYEVVDLGVDVSAGRILQAVKETGAGIVGLSVLLSTMVGGIRTV
                     VEEAKAHGIRDRIKIVIGGACTSRALCEEMGADAYGENAMDALRIVNGFSENA"
     gene            4951..5922
                     /locus_tag="PLT69_03225"
     CDS             4951..5922
                     /locus_tag="PLT69_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ13485.1"
                     /translation="MDTAKPYLVAPVASCLGARLTRTSMEDNILSPRVQVSSILALVE
                     RFPGIDLVFPFMDTSVEARALGCPHDFKGRVPVVSAHPFADPGQIAGIETPDPYESEP
                     MHNMIEVTGEIARVCERPVMSFVVGPVTLAAHLMGVTALVRLAAGNPAAFSEVLENCV
                     RIVEPYGRALASSGASGVVILEPQLVFFPPRTYEQAVRPAIERLARALPRPALHVCGD
                     TRRHLAAFGLTRHVECLSLDEAVDFAQAKDSIPELEGKTLMGNIPAVNVMHRGTPEAV
                     RSAVERLVGSMQGTFFILSTGCDMVPDTPLENMEAFMQAAARPSAAS"
BASE COUNT         1232 a         1739 c         1855 g         1154 t
ORIGIN      
        1 acaagctcag gcgctcatgt cccatatccc ccgagaggga agggcgcctg caggatccgg
       61 cccccgtcgc ccaacaaggc atgccacttc tggctgcggt cggtctgccc gggagaaccg
      121 ctccgggatc gagggctgta atcctcgccc cacggactgg tcctcgaaga tgttgacctg
      181 cccgggagaa ccgctcctgg atcgaggtcc ctctagaggg agacggcagg cgcttggata
      241 aggggcatcc tgggcgcccc aggcgcagcg cggcaagccc ctctcccgag ggatgacggc
      301 gggtcctgcg gcgggcggac ggcctggcct tccggtgact ctatgggaag aatggcaata
      361 ccacctaagg ggtaatggaa acgaggcgta atttatggca ttaaagacga atggctatgg
      421 atattgaatc tatcatcatc cagtatggct acattttcat cctgatcggc acattctttg
      481 agggggagac cgtactcgtg ctggcgggtt ttctggtcca ccagggatac ctttccttta
      541 caccggttgt cgctgctgca ttcatcggaa cctatgttgg ggatcagctc tatttttaca
      601 taggaagaag aaagggcatt gatctcctcg atcgtaaccc gtcatggaag cggcagtcag
      661 gtagagtatt cccgctgctt caacattatc agaccatggt tattatcgga ttcaggttta
      721 tatatggaat ccgcaccgtg accccgttta ttttaggggc aagcggcgta tcacccagga
      781 gattcatggt cctgaatgcc gtaggaggta tcgcctggtc actgggtata gcatccctgg
      841 ggtatatgct cgggtatacg gcggaagtca taatccagga cataaaacga tatgaataca
      901 tcatcatggc atcaattgta atggccggca ttgttttctg ggggatatat ctgataaggc
      961 gcaaaaggcg gggggtctaa ccggtttttc gtttctctct ttcctctaca aattgaagcg
     1021 gtgaaggaaa tacaatgtgg gaggggatcc agtcatgggt gacatgatat caaaggtcag
     1081 acgtacctgt cagatatgcg gggcaggtga ggatgtcgcg gaactgagac ccgggattat
     1141 ggtcaggcct gctataggcg agctcattta aagtgatcat ggcaaatggg atgagcaggg
     1201 ttggatttgt gtcggtgacc tgcaaacata catgcacaga tacgtacaga atctcatgga
     1261 agaggagaag ggggaactga cggagataga tcatgaggtg atcgagagta taaaagaaca
     1321 gaacatcctc tcaaaaaatc ccgatgaagg cttcgaggca aagctcacca tgggtgagcg
     1381 tttagccgac cgcatagcgg atttcggcgg gagctggacc ttcctgggca tctttgcact
     1441 ggtcattctc ggctggatgg ttattaattc ttatgtcttc atcgcccgtc ccttcgatcc
     1501 gtatccgtat atcctcctga acctggtgct ttcatgcctt gccgctgcac aggcgccggt
     1561 catcatgatg agccagaacc gccaggaggc cagggacagg gagcgggcac tgcacgatta
     1621 ccaggtcaac ctgaaggccg aactggaaat ccgccacctc catcagaagc tcgaccacat
     1681 catgtcacac caatggcaac gtctggtgga gatccaggag gtccagatgg atatcatgaa
     1741 tgaattgcga agcagaaagc cttgaacgaa cagctttcaa agaattgacc cggctgatat
     1801 tagtaaagtc ccccaatttc tgtaataagc atctccgata gaggcatcat gcagccagca
     1861 gattctccgt ctacatgccc gatatctcat cttggaagat gatgtatcgg gcgagaagac
     1921 gatgtaatgc cccctggaat ctctaccaat catgcggctc atttcccctt gttgtcaggc
     1981 gacaggataa agtggcccct ggacgacatg agctccattc gtccaagcct ggtcatcagg
     2041 accctgggct tgtacctgtt ccggtagccg gcacgctgcc cggtgttctc ttgagatccg
     2101 gccttaaggt atgccgtcag ctcttcttcc agcaccatct gcacgccata ctccatcaac
     2161 ctcaaaagac cgtcttcccc cctcgccggt cgccacttgc cacaggcccg acaacccctg
     2221 aacgtctcct ccacgagcca tacgcacctc cttacgagcg tcgtttcccg ctacgccgag
     2281 tatcggaggt gcccttggct cgctttcacc ttttttacag aacgagagag acggaaccgg
     2341 ggatctctat cacgcacaga gatagtctct gtggagcatt atcttgctta ctaaatcttg
     2401 ataactcatc gtacacccct ccagaaagga tacgtcattc cgatagagcc aagatatcgc
     2461 cggaactggt cagaccaatt ttgacttgac aacaagccca tcccaattca tactgcatgg
     2521 tcatatgaca agacgccggt tggacaaggg gatgcccggc ggttttcagg aggcgcgtgc
     2581 gccccgggcg ggcgagtgat ccccaaggag gcgcggtatc gagacagggg tgcagagccg
     2641 ggagggtggt acccgtcggc ctgccgggcg gacaacggcc gtgtgcgctc cattggggcc
     2701 cggggaacat gctcgtgacg caagggccgc tccggccggc cgtgcatggg ccctcgcttc
     2761 gggatatcca cgggtgggtg ctgcgcggag gccgggcgcc gcaagggctc gtgccaaagg
     2821 ggcctgatgg ttcttgaact gcgccgtatg gagctctcct taagcgcagc cccacgggga
     2881 cggtatctgg tgaggttttt aaggtatagg gcaaggcctg gactgttgcc tgctgcgcgg
     2941 atgccccgcc acaagggttc atgtcgaagg ggactgatgg ttcttgatcc gcgctgttgg
     3001 gcgccttctg tgagcgcggc tgtgcaggga tggtgcctga tggggattgt agggtttcgg
     3061 acagggcctg ggcggaaggg atcgaccgcg atccgggccg catgatcagg ataggaggtt
     3121 cacatgcacc ctgtcgagcg cgtcctgagg acgttcgagg gcaaggagac ggaccgcgta
     3181 cccgccttct gcgccatggt ggagggacgc acgtacaacg aggtgctggg caagccgctc
     3241 gtgtcccagg agaggctcct ggtgaacccg gtggccacgt tcgccctcga ccggtggggg
     3301 ccccttctca ccaagctcgt gttccagcac gaggtgaccc ggagcatgga aaagcgcatc
     3361 aaggcccagg tggagcttgg cttcgacgcg gtctgggcca tcctcgacga gaccttcatc
     3421 gtgactgacc accgcaccat ggtccgcttc acggggtcac ggtacaacct gcaaccggac
     3481 ggctacggca acatgacgta catgtaccgg gagccgtgct tcaagacccg ggaagacttc
     3541 ctggagtggc cgtactggcc ggacgccgac gacgtggcgc acagcgccta ccgtttcttc
     3601 agcgccatga tgaagaaata cggccacagg gcctgcctct tcgggcaggc atccgcctac
     3661 ggcatacagg aatcgctcct gtgggccgtg ggcttcgaga ggctgccgct ctggatcagg
     3721 cgcgagaggg atctcgtcaa gaggttcatc gagatcaacg aggagctctg cctgaagacg
     3781 aacatggccc tcctggacgc aggggtcaag gtagtcatcc agacggacga cttcgccttc
     3841 aagagcgggc cgatcatgaa cccgaggctc atcgacgagc tcttcggccc ctcctacagg
     3901 cgcatcatca aggcggtgca cgacaggaac ggcaagtacg tcctccactc ctgcggcgac
     3961 aacaccctgc tcttcgacac cttcatctcg tggggcgtgg acggcctgca cgcctacgag
     4021 accacctcga acgtggacat cttcaaggaa aaggaactcc acggagacaa ggtcacgatc
     4081 atcggcgggg tgggcgttga ttaccttctc accgaccggt cgcgcgacga ggaggtggtg
     4141 cgggaggtca ggcgcctcat cgcgaggctg gggccttcgg gcaggttcat catatcgccg
     4201 gtgcacagcc tgagcaccat cccggcccac aagctgaggg tcatggtcga tgcggtgcgc
     4261 acctacggga ggtaccccct gtcaccggca ctcgaggagg acgcgggatg aacgattccc
     4321 tgagactggt atgcgaggcg gttgacaccc tggatatcga cggcatcagg gacctggtgc
     4381 agagatgcct ggaagagggg cacacaccgg aggagatcat cgagcacggc atcggcaagg
     4441 gccttgacct cgtggggaag aagttcgagg cgggcgagta cttcctcgca gacctggtca
     4501 tggccggcga ggtggtcaag gaggtcatgc ccgtgctcaa ggagcgcatg aacccggtag
     4561 atgcacccag gcggcagaag gtgatcctgg cgacggtgga gggagacatc cacgagatag
     4621 gcaagaacat cgtgggcatc atcctgtccg ccaacgggta cgaggtcgtg gacctcgggg
     4681 tggacgtgag cgcagggcgt atcctccagg cggtcaagga aacgggggcc ggtatcgtgg
     4741 ggctctccgt gctcctctcc accatggtgg gaggcatccg caccgtggtg gaagaggcga
     4801 aggcccacgg catcagggac aggatcaaga tcgtcatagg gggtgcgtgc acctcgaggg
     4861 ccctgtgcga ggagatgggc gccgacgcct acggcgagaa cgccatggac gccctcagga
     4921 tagtcaacgg cttctcggaa aacgcctgag atggacacgg cgaaaccgta cctcgtcgcg
     4981 cccgtggcct cctgcctcgg cgcccggctc acgaggacgt ccatggagga caacatcctc
     5041 tccccccgcg tgcaggtctc gtcgatcctc gccctcgtcg agcgtttccc cgggatagac
     5101 ctggtgtttc ccttcatgga cacgtcggtg gaggcccgcg ccctcggctg cccgcacgat
     5161 ttcaagggca gggtgccggt cgtctcggct cacccgtttg cggaccctgg ccagatagcc
     5221 ggcatcgaga cccccgaccc ctacgagagc gagcccatgc acaatatgat cgaggtcacg
     5281 ggcgagatcg cgcgggtgtg cgaaaggccg gtcatgtcct tcgtggtcgg ccccgtcacc
     5341 ctcgccgccc acctcatggg ggtgacggcg ctcgtcaggc tcgcagccgg taaccccgcc
     5401 gccttctccg aggtgctcga gaactgcgtg cgcatcgtgg agccctacgg acgggccctg
     5461 gcttcaagcg gcgcctcggg cgtggtcata ctggaacccc agctggtctt cttcccgccc
     5521 aggacctacg aacaggcggt caggccagcg atagaacgtc tcgctcgtgc gctgccgaga
     5581 cctgcgctcc acgtctgcgg cgacacgagg cgccacctgg cggccttcgg cctcacgcgc
     5641 cacgtggagt gcctgagcct ggacgaggcg gtggacttcg cccaggcgaa ggactcgata
     5701 ccagagcttg agggcaagac cctcatgggg aacatcccgg ccgtgaacgt catgcacagg
     5761 ggcacgcccg aagccgtgcg cagcgccgtg gaaaggctcg tcggctcgat gcagggcacc
     5821 ttcttcatcc tgtcgaccgg ttgcgacatg gtgccggaca ccccccttga gaacatggag
     5881 gccttcatgc aggcggccgc gcggccatcg gcggcgtcat gacgccatga gagagtacac
     5941 ggtcaccctt gtcccgtcac gccggtcccg tcccttcccc
//
LOCUS       DAOSCR010000078         5958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8462_length_5958_cov_2.817381, whole genome shotgun sequence.
ACCESSION   DAOSCR010000078 DAOSCR010000000
VERSION     DAOSCR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_5958_cov_2.817381"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            563..1723
                     /locus_tag="PLT69_03230"
     CDS             563..1723
                     /locus_tag="PLT69_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ13486.1"
                     /translation="MMMKIVKYVLVAGALVFLASPTLSAADKEPVVIGLQGPITGAWA
                     YEGQMAKQACEVAADLINKKGGILGGRKIVIKVEDDAGEPKTGALAAQKFVGQKDVVA
                     VVSTYGSSVCEPASNIYEKFKKVNIGYGVTAVRLTERGFKYFFRTCGRDDSQGKFFAE
                     AVPKKFKARRIAIMHDNTAFGKGLAEDTKRALEPMTKANKVEIVYFDAITPGEKDYKV
                     PLTKMRETKPDVWYYTGYYAEAALLVSQARDIGITCPFVGGNAAINDEFVKIAGLNVA
                     KGCYMTNEPLPGDLPYKEAREFLAAYKAKFGDIPSSPWPIYAADAVNIIAYAIDKTKS
                     TDSDKIASFLRDKVDRVPGITGPIGFTAQGDREGVPFYLYVVNNEGKIVISK"
     gene            1751..2662
                     /locus_tag="PLT69_03235"
     CDS             1751..2662
                     /locus_tag="PLT69_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ13487.1"
                     /translation="MHLVLEQIINGLTIGSFYALIALGYSMVYGVLKLINFAHGDFFA
                     MGSYIGYTVLVFHVAGATVSMGPWAGMGLAVLTAGVSLMAVGIAVERAAYRPVYRAGR
                     LPAVVSALGASIFLQNVIMLVWGPRYQAYPADLIPAARIHFGDLTVTMLQLVILALSF
                     ALMSALYYVIQKTTFGAAVRATAHDRNTAALMGINFNRVIVFIFTLGPALGAVAGVMV
                     GMYYRQISFTMGWNYGLKAFTATILGGIGNLPGAMFGGLILGVLEMLGAAYISAAWKD
                     VFVFLILILVLIVRPTGLFGERTAEKV"
     gene            2665..3666
                     /locus_tag="PLT69_03240"
     CDS             2665..3666
                     /locus_tag="PLT69_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ13488.1"
                     /translation="MRDLQNLSGGGLRSARLLAPLAFACLVTAYPFAVSDYLIDIGFF
                     FGIYALLGLSLNIVLGEVGLFDLGHAGFYAIGAYVTAVLNTRYQVPVLVLMPVSCLAA
                     ALFAYLVCSPIIHLKGDYLCIVTIGMGEIVRLALINNPFDLTGGPNGVFGIDFPRVGN
                     LLVVDSSIEYYFLVWITVGVSIAGLVSLQHSRIGRAWNCIREDAVAAQATGINVRAYK
                     LLAFVLGAALAGAAGNIYASKLMCVSPESFTFWESCLMFCIVLIGGMGSIPGVIVGAA
                     FISLFPEIFRPFAMYRMLVFGSIMIVMMMVRPGGFWPRKRGAVTLRPLMEDTADGAV"
     gene            3653..4455
                     /locus_tag="PLT69_03245"
                     /pseudo
     CDS             3653..4455
                     /locus_tag="PLT69_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716443.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4457..5191
                     /locus_tag="PLT69_03250"
     CDS             4457..5191
                     /locus_tag="PLT69_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13489.1"
                     /translation="MAPLLGIEDLHISYGAREVLKGVDVRIEEGEIVTVLGANGAGKS
                     TLLQAICGLVPVASGAITYDGRRLNGMPPYRIVSMGISLSPEGRKVFPTHTVEENLYL
                     GAFTRRGDKSGIEQAKRRVHALFPILEERKKQLAGTLSGGEQQMLAIGRALMSNPRVL
                     LLDEPSLGLAPILVKQIFDIVREINSQGTSILLVEQNAHKALSLASRGYVLENGSIAA
                     HGPANVLASDERIREAYLGGSALRKA"
     gene            5682..>5958
                     /locus_tag="PLT69_03255"
     CDS             5682..>5958
                     /locus_tag="PLT69_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13490.1"
                     /translation="MDTTASPNTHTLDQWASLFNPFWSLLSAMTRKAGETEAPEEAGL
                     TDSHVKTSQEALLAIFKTWSAFTETLSEPSSLTAVTGTVGKADFAGCA"
BASE COUNT         1091 a         1956 c         1900 g         1011 t
ORIGIN      
        1 cctcgaaccc atagccgcgg ccgtgatggc cttttccctc ctgggagagc ggctgtccgt
       61 cctccaggcg gcgggcggcc ttgccgtcat ctcggccgtc gtcctgctcc agctggaacg
      121 cgagcacgac gagcgctccc ccgagaccat cagggcgacg gcgagagacc ggcgttgagg
      181 gtgcaagggg cggcgcaaac ggccttggtc gtacctgctc gatgcctgcc tggggggagt
      241 cacgacccgg cacgcgcagg gtgacgtccc ggcgcgcgat cacgtgcggg aacggatgca
      301 ggggtctttc tgggccgcgc ccggggagtc cctgggtcga aagaccggcg gcgggcaaaa
      361 gacagccggg caccgcaggc ggcccgtgtc ggggagattg ccgttgacac tgccgctcgc
      421 tcgctcatat acttcacttc ccacactgca tcgggtgttc cccgggttcg gagcacgcac
      481 gagtcacgcc ctgggcacca tctcgcatgc cggtgtgaca accggatgga agcggttgta
      541 caaatccttt gtacgagggg ggatgatgat gaagatcgtg aagtacgttc tcgtggcagg
      601 cgcactggtg ttcttggcct cacccaccct gagcgccgcg gacaaggaac ccgtcgtcat
      661 cggcctgcag ggccccataa ccggggcctg ggcctacgag ggccagatgg ccaagcaggc
      721 gtgcgaggtt gctgccgatc tcatcaataa gaagggcggc atcctgggcg gccgcaagat
      781 cgtgataaag gtcgaggacg atgccgggga gccgaagacc ggcgcgctcg cggcgcagaa
      841 gttcgtgggc cagaaggacg tggtcgccgt ggtgtcgacc tacgggtcgt cggtgtgcga
      901 acccgcgtca aacatctacg aaaagttcaa gaaggtgaac atcggctacg gggtcaccgc
      961 cgtccggctc accgagcgcg gcttcaagta cttcttccgc acctgcggca gggacgactc
     1021 gcagggcaag ttcttcgcgg aggcggtgcc caagaagttc aaggcaaggc gcatcgccat
     1081 catgcacgac aacaccgcct tcgggaaggg cctggcggaa gacacgaaga gggcgctcga
     1141 gcccatgacc aaggcgaaca aggtcgagat cgtctacttc gacgccatca cgcccgggga
     1201 gaaggactac aaggtgccgc tcacgaagat gcgcgagacc aagcccgacg tctggtacta
     1261 cacgggctac tacgccgagg ccgcccttct cgtctcccag gcgcgcgaca tcggcatcac
     1321 ctgcccgttc gtcgggggca acgcggccat caacgacgag ttcgtcaaga tcgcggggct
     1381 caacgtggcc aagggctgct acatgaccaa cgaacccctg cccggggacc tgccctacaa
     1441 ggaggccagg gagttccttg cggcctacaa ggccaagttc ggcgacatcc cctcgagccc
     1501 gtggcccatc tatgccgccg acgccgtgaa catcatcgcg tacgccatag acaagaccaa
     1561 gtccaccgac tcggacaaga tcgcctcctt cctcagggac aaggtcgaca gggtcccggg
     1621 catcaccggc cccatcggct tcaccgccca gggggaccgc gagggggtgc ccttctacct
     1681 ctatgtggtg aacaacgagg gcaagatcgt gatctcgaag tagagacctc gtaaagagcg
     1741 cgtccgagaa atgcacctgg tgctcgaaca gatcatcaac ggcctgacca tcgggtcgtt
     1801 ctacgcgctg atcgcgctcg gctactcgat ggtctacggc gtgctcaagc tcatcaactt
     1861 cgcccatggc gactttttcg ccatgggctc ctacatcggg tacacggtcc ttgtcttcca
     1921 cgtggccggc gcgaccgtga gcatggggcc atgggcgggc atggggctcg cggtgctgac
     1981 ggccggggtg agcctcatgg ccgtcgggat cgcggtggaa cgcgccgcct accgccctgt
     2041 ctaccgcgct ggaaggctac cggcggtggt gtcggccctg ggggcatcca tctttctcca
     2101 gaacgtgatc atgctcgtct ggggacccag gtaccaggcg tatcccgccg atctcatccc
     2161 ggccgcacgc atccatttcg gcgacctcac cgtcaccatg ctccagctgg tcatcctcgc
     2221 cctctccttc gcgctcatgt ccgctctcta ctacgtgatc cagaagacca cgttcggcgc
     2281 cgccgtcagg gccacggcgc acgacaggaa cacggccgcc ctcatgggca tcaacttcaa
     2341 ccgggtgatc gtgttcatct tcacgctcgg accggccctc ggggcggtcg ccggcgtgat
     2401 ggtgggcatg tactaccgcc agataagctt caccatggga tggaactacg gcctcaaggc
     2461 cttcaccgcg accatcctgg gcggcatagg caacctgccc ggcgccatgt tcggcggtct
     2521 catcctcggg gtcctcgaga tgctgggcgc ggcatacatc tccgcggcct ggaaggacgt
     2581 gttcgtgttc ctgatcctca tcctcgtgct catcgtgagg cccacgggcc tcttcggcga
     2641 gaggaccgcc gagaaggtgt gaccatgagg gacctgcaga acctttccgg cggcgggctt
     2701 cgctcggcac gcctcctcgc gcccctggct ttcgcctgcc tcgttacggc ctacccgttc
     2761 gcggtgagcg actacctcat cgacatcggc ttcttcttcg gcatctacgc cctcttgggc
     2821 ctgagcctga acatcgtcct cggcgaggtg ggcctcttcg acctgggtca cgcagggttc
     2881 tacgccatag gcgcgtacgt gacggccgtc ttgaacacgc gctaccaggt gcccgtcctc
     2941 gtcctcatgc cggtgagctg cctggccgcc gccctcttcg cctacctcgt ctgttccccc
     3001 atcatccacc tcaagggcga ctacctctgc atcgtgacca taggcatggg cgagatcgtg
     3061 aggctggccc tcatcaacaa ccccttcgac ctcaccggcg gcccgaacgg cgtcttcggc
     3121 atagacttcc cgagggtggg gaacctcctc gtggtcgact cgtccatcga gtactacttc
     3181 ctcgtgtgga tcacggtggg ggtctcgatc gccgggctcg taagcctcca acactcgaga
     3241 ataggcaggg catggaactg catcagggaa gacgcggtcg cggcgcaggc gacgggcatc
     3301 aacgtgaggg cgtacaagct gctcgcgttc gtgctcggtg cggccttggc cggcgcggca
     3361 ggaaacatct acgcgagcaa gctcatgtgc gtctccccgg agagcttcac cttctgggag
     3421 tcgtgcctca tgttctgcat cgtgctgata ggcggcatgg gttcgatccc cggggtgatc
     3481 gtgggcgccg ccttcataag cctgttcccc gagatcttcc gtccgttcgc catgtaccgc
     3541 atgctcgtgt tcggatcgat catgatcgtc atgatgatgg tccgccccgg ggggttctgg
     3601 cccaggaaac gcggagcggt gacgcttagg ccgctcatgg aggacaccgc ggatggggct
     3661 gtctagggcg cacgcggcaa acgacacggt gctctccacc aagggcctca ccaaggtttt
     3721 cggcggcctc aaggcggtgg attccctcga cctggaggtg cacagggggg agatcctgag
     3781 cctcataggc cccaacggcg cgggcaagac cacggtgttc aacgtcgtta ccgggatcta
     3841 cacccccgag gcaggggagg tgatcttcga cggccgccca tcacgggcat gaagcctcac
     3901 cggatcgtgt cgctggggat cgcgcgcacc ttccagaaca tcaggctctt cccggacatg
     3961 acctgcctcg agaacgtgat ggcgggccag cacgtcagga gccgggccgg ctttctctcc
     4021 tcgctgttca ggacgcccgg catgcgcagg gaggaggcgc agatcgagcg gaccgccatg
     4081 gagcggctgt cccaggtggg cctggagcga ttcgcgcacg atctggccaa gaacatcgcc
     4141 tacgggtccc agcgcatgct cgagatcgca cgggcgctgg ccaccgagcc gaggctgctg
     4201 gtgctcgacg agccgagcag cgggctcaac cccaaggaga ccagggatct gatgtctttt
     4261 ctcgagggac tcatccaaaa cgacgacctc acgatcctcc tcatcgagca cgacatgaac
     4321 gtggtcatgg gcatatcgga ctgggtggtg gtgatggacg acggcgtcaa gatcgccgag
     4381 ggaaggcctg aggacatcta tcacgaccct ctggtgatcg aggcctacct cggcagggaa
     4441 agcgaggaag gctgagatgg cccccctgct cggcatcgag gatctccaca tctcgtacgg
     4501 ggcccgcgag gtcctgaagg gggtggatgt ccgcatcgag gaaggcgaga tcgtgacggt
     4561 gctgggggcg aacggcgcag gcaagtccac cctgctccag gcgatctgcg ggctcgtgcc
     4621 cgtggcatcc ggcgccatca cgtacgacgg ccgccgcctg aacggcatgc ccccgtaccg
     4681 gatcgtttcc atgggcatct cgctctcccc cgagggcagg aaggtctttc ccacccacac
     4741 cgtggaggaa aacctctacc tgggcgcatt cacgcgacgt ggcgacaaga gcgggataga
     4801 gcaggcaaag cgtcgcgtgc acgccctctt cccgatcctc gaggaacgca agaaacagct
     4861 cgccgggacc ctctccggcg gcgagcagca gatgctggcc atcggcaggg cgctcatgag
     4921 caacccgagg gtcctcctcc tggacgagcc ctccctcggg ctggcgccca tcctggtcaa
     4981 gcagatcttc gacatcgtga gggagatcaa ctcgcagggc acctcgatcc tgctcgtgga
     5041 gcagaacgcc cacaaggcct tgagcctcgc atcgaggggc tacgtgctcg aaaacggcag
     5101 tatcgcggcc cacggcccgg cgaacgtcct ggcgagcgac gagcggatcc gggaggccta
     5161 cctgggcggc tcggccctca gaaaggcatg aatccaccgc tcccccgtgt ccacgacgac
     5221 gggggacgac acccgacaaa gcccggccaa ggccgacgct catcaccgac ccatccagtc
     5281 catgatgcac cacaaccgtg cgggtacgtc cgggagcatg ctcgcccgat gaaatgtgca
     5341 aggcgtcttc ggccaggtcg ggcgcatcgc agcgcgaagc gaaccgtgtt cgcgagccct
     5401 gttctctcgg cgaggtcggt cacgcacatg ctgtcagtat gtcccggctc ttcgatacgg
     5461 acagggctcg gcccctccca tgaccgagac acggcgctca catggacgta cggacagggg
     5521 acgttgtgga cgaggtgaac ttggggacat tcctcacgag tcttgacagc acagcctgta
     5581 cgtattgact cgcgcgcccc gggcattact cttgagagaa ggacgaggct tcggcgcacc
     5641 gtccggagaa caggaggcca cggcaaagac ccggagagga catggacacg acggcaagcc
     5701 caaacaccca taccctggat cagtgggcca gtctcttcaa tcccttctgg tcgctcctgt
     5761 cggcgatgac ccggaaagcg ggggagaccg aggcccctga agaggcgggc ctgacggaca
     5821 gccacgtgaa gaccagccag gaagccctgc tcgccatctt caagacatgg tccgcattca
     5881 ccgagaccct ctccgagccc tcgtcgctga ccgccgtgac cggcacggtc ggcaaggcgg
     5941 acttcgccgg ctgtgcga
//
LOCUS       DAOSCR010000079         5947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8484_length_5947_cov_3.519348, whole genome shotgun sequence.
ACCESSION   DAOSCR010000079 DAOSCR010000000
VERSION     DAOSCR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_5947_cov_3.519348"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>210)
                     /locus_tag="PLT69_03260"
     CDS             complement(<1..>210)
                     /locus_tag="PLT69_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOJ13491.1"
                     /translation="ALEYVENKVRGVEVDKRFVYYLLGSKGICVVPLTGFCCSRKGFR
                     ITLLECDDEKRRWTFTTIAEAIRDY"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<221..1282)
                     /locus_tag="PLT69_03265"
     CDS             complement(<221..1282)
                     /locus_tag="PLT69_03265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ13492.1"
                     /translation="MRRNIVHEGAEQLTYEIRQIVAVAEDLQRLGVEITWENIGDPIA
                     KGEKLPTWMKEIIVSLVHDDRTYGYVATQGVAHARQFLADMVNKRGGYQVTKDDIIFF
                     DGIGDAIAKIFGYLKREARVIGPSPAYSTHSSAEAAHSGYDHLTYELDPTNNWMPDLD
                     DLEKKIKYNDSIAGILIINPDNPTGAVYPRDLLVEMVNIARRYKVFMICDETYMRLVY
                     GGVETAHLSEVIKDVPGIALRGISKEYPWPGARCGWIEVFNQDKLNFKRYIKSLVNAK
                     MLEVCSTSLPQYSILLIMSDPRYEEHLASRRAMYEKRAQEAYEIFSKVEGIHVNKPQG
                     AFYLSVVFEDGVLNNRQTLP"
     gene            complement(1396..2931)
                     /locus_tag="PLT69_03270"
     CDS             complement(1396..2931)
                     /locus_tag="PLT69_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13493.1"
                     /translation="MRIEPSRPDMTAGTNRAVDIPFGLRILVADASLVLRSVLSWSLE
                     RHGARIWHAESSSQALDKIGAMDFDYVIADAELPDSGGTELCRAIKSDRRTQHIPVII
                     LDSRDSEDVQKAAYQAGASACISKNQTKNGILEDLLHTMKQTAIKHNRLVLVVDDSLT
                     VRSLVSKGLKEAGFRVVEAENGRAALDVLRDVKPDIILSDIDMPEMNGEGFCRAVHED
                     PVYASIPFVVMSTHSERLVMRRMLQIGADAYLVKPFNVEQLVITLEKLLSDQLLLLQK
                     EKERLESEQKLMVASITSLCAALEARDSYTRGHSEAVSTFASRIAREMGLSEEEVELV
                     RLGGRLHDIGKIGVPDHILLKPGRLSEEEFSIIKKHPVIGGEILRPVPSLAAVLPIVI
                     HHHERLDGKGYPEGLHGSSIPLWARITAVCDTFHALTSDRPYRNGMPKDKAVEIIQQV
                     KGTQLCPDCVDAFIKTCIWEAGVPGTQADEPEDDQREGSNTHRFSHHPPRVTETGHTT
                     EHA"
     gene            complement(3357..4427)
                     /locus_tag="PLT69_03275"
     CDS             complement(3357..4427)
                     /locus_tag="PLT69_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ13494.1"
                     /translation="MKHVLRRWLRRYFSDPQVLILILILVAGFVVFSLFSRMLAPVLV
                     AVVLSVLLDSLITWLMRFNVRRETLVYLVFLLFVFVFVDLLLIVLPLVWRQVSELIRD
                     LPGMVSTIKATVSTLPQKYPGVITEETVDRALAILSEEIEGIGGGIVKLSVVSAKGVV
                     EIVVYLVLVPFLVFFMLKDKKLILDWLSGFIPEERGLAVLVWDEVYDQFFNYLRGKVL
                     EIMFVWAINYAAFAALGLKYSVILSMLVGLAVLVPYIGAAVMFVPVVLVAFFQWGLGA
                     HTTWTVIAYLALHALDGNLVAPLLLSRVVRIHPVAIIVAILVCGGLWGFWGLVFAIPL
                     ATLMHAVLRAWKGTAEATAEQA"
     gene            complement(4519..5082)
                     /locus_tag="PLT69_03280"
     CDS             complement(4519..5082)
                     /locus_tag="PLT69_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOJ13495.1"
                     /translation="MVLKRAGFLAVALVFLATSAFAVDIVGVDMPDTLKAGDTSLVLN
                     GGGKRTKFGMKVYVAALYLKQKSADANAIINADEPMAIRLQITSGLVTPDKMKAAIDE
                     GFEKSTGGNVAPLKAKIDAFTACFRGLAKDDVYDFVYQPGKGIEIMKAGKLVSVIQGL
                     DFKKALFGIWLGKEPAQDSLKENMLGK"
     gene            complement(5218..>5947)
                     /locus_tag="PLT69_03285"
     CDS             complement(5218..>5947)
                     /locus_tag="PLT69_03285"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOJ13496.1"
                     /translation="GGSTRENTHRLVVRLNSQTDLTVVSHLTCVESTDKDIMAILERY
                     HASGIENILALRGDPPAGHARWDPAPGGFAHAIDLVGFIKRRFPGMCVGVAGFPEGHP
                     ETPNRLREIAYLKAKVDAGADYIITQLFFDNRDFYDFCERCELAGIHVPIVAGVMPIV
                     SVSSMERMAELAAHARFPAPLLRAVYSAPSPEAVEHAGVTWAIDQVRDLVSHGVKGVH
                     FYTLNNARATLRIYKELGLGPKSP"
BASE COUNT         1022 a         1837 c         1927 g         1151 t
ORIGIN      
        1 tgtagtccct gatggcctcg gctatggtcg tgaacgtcca cctgcgtttt tcgtcgtcgc
       61 actcgagcag ggtgatcctg aaacccttgc gcgaacagca gaagccggta agcggcacga
      121 cgcagatgcc cttcgagccg aggaggtagt acacgaagcg cttgtccacc tccacgcccc
      181 tgaccttgtt ctcgacgtac tcgagggcct nnnnnnnnnn gggcagcgtc tgcctgttgt
      241 tgagcacgcc gtcctcgaac acgaccgaca ggtagaaggc tccctgcggc ttgttcacgt
      301 ggatgccctc caccttcgag aagatctcgt aggcctcctg cgccctcttc tcgtacatgg
      361 cgcgcctcga tgccaggtgt tcctcgtacc gcgggtcgga catgatgagg aggatggagt
      421 actggggaag gctcgtggaa cagacctcga gcatcttcgc gttcacgagg ctcttgatgt
      481 agcgcttgaa gttgagcttg tcctggttga agacctctat ccagccgcag cgagcgccgg
      541 gccatgggta ttccttggat atgcccctga gcgcgatgcc gggcacgtcc ttgatgacct
      601 cgctcaggtg ggccgtctcc acgccgccgt agacgagcct catgtaggtc tcgtcgcaga
      661 tcatgaagac cttgtatcgc ctggcgatgt tgaccatctc gaccaacagg tcgcgcgggt
      721 agacggcgcc cgtggggttg tccgggttga tgatgaggat gcccgctatg gagtcgttgt
      781 acttgatctt cttttcgagg tcgtcgaggt cgggcatcca gttgttggtc gggtcgagct
      841 cgtaggtgag gtggtcgtag ccagagtgcg ccgcctctgc ggaagagtgg gtggagtagg
      901 ccggtgacgg cccgatcacc ctggcctcgc gcttgaggta accgaatatc ttcgcgatag
      961 cgtcgccgat gccgtcgaag aagatgatgt cgtccttggt gacctggtaa ccaccccttt
     1021 tgttcaccat gtcggccagg aactgcctcg cgtgggcgac gccctgggtg gcgacgtacc
     1081 cgtaggtcct gtcgtcgtgg acgagagaga cgatgatctc tttcatccag gtggggagtt
     1141 tctcgccctt cgctatggga tcgccgatgt tctcccaggt gatctcgacg ccgagacgct
     1201 ggagatcctc ggccacggcc acgatctgcc tgatctcgta ggtgagctgc tcggcaccct
     1261 cgtgaacgat gttccttctc atggtatcct gccacgctcc ttgaggcctg tgcgggcgtc
     1321 ccgcacgtcc ctcgtttgct gggaagataa acaaaacatc ggccgaatgc aatgcgattc
     1381 ttgagggttc aggcctcagg cgtgctccgt ggtatggcct gtttcggtca cccggggagg
     1441 gtgatgggag aaccggtggg tattggaccc ttcccgttgg tcgtcctcgg gttcgtcggc
     1501 ctgggtgccg gggacgccgg cctcccagat gcacgtcttg atgaaggcgt ccacgcagtc
     1561 ggggcagagt tgggtgccct tgacctgctg gatgatctcg acggccttgt ctttcggcat
     1621 gccgttgcgg tagggcctgt cgctcgtgag cgcgtggaag gtgtcgcaga cggccgtgat
     1681 ccgggcccac agggggatcg aggaaccgtg caacccttcc gggtaccctt tcccgtcgag
     1741 gcgttcgtgg tggtggatga cgatcggcag gacggccgcc agcgatggta ccggcctgag
     1801 tatttcaccc cctatcaccg ggtgcttctt gatgatggag aactcttcct ccgagagcct
     1861 gcccggtttc agcaggatat gatccgggac gcctatcttg ccgatgtcgt ggagcctgcc
     1921 cccgagcctc accagttcca cctcctcctc ggagaggccc atctcccggg cgatcctcga
     1981 tgcgaaggtg ctgaccgcct ccgagtgccc cctcgtgtag gagtcacgag cctccagggc
     2041 ggcgcacaga ctcgtgatgc tcgccaccat gagcttctgc tctgactcga gccgctcctt
     2101 ctccttctgg aggagcaaca gctggtcgga gaggagtttc tccagcgtga tgacgagctg
     2161 ctcgacgttg aagggcttga cgaggtacgc gtcagcgcct atctggagca tccgcctcat
     2221 gaccaggcgc tcgctgtggg tgctcatgac cacgaacggg atggaggcgt agacggggtc
     2281 ctcgtgaacc gccctgcaga agccctcgcc gttcatctcc ggcatgtcga tgtcgctcag
     2341 gatgatgtcc ggcttcacat ccctgaggac gtcgagggcg gccctgccgt tttcggcctc
     2401 gacaaccctg aagcccgctt ccttgaggcc cttgctcacg agagaccgca ccgtcaagga
     2461 gtcgtcgacc acgagcacga ggcggttgtg cttgatcgcc gtctgcttca tggtgtgcag
     2521 gaggtcttcc agtatcccgt tcttggtctg gttcttggag atgcaggcgc tggcgcccgc
     2581 ctggtaggcg gccttctgga catcctcaga gtcgcgcgag tcgaggatga tcaccgggat
     2641 atgctgggtc ctccggtcgg acttgatcgc cctgcagagc tctgtcccgc ccgagtcggg
     2701 cagctccgcg tccgcgatca cgtagtcgaa gtccatggcg ccgatcttgt cgagggcctg
     2761 gctcgagctc tcggcgtgcc atatcctcgc gccgtggcgc tcgagcgacc acgacaggac
     2821 gctcctgagc accaggctcg cgtccgcgac gaggatcctg agtccgaagg ggatgtccac
     2881 ggctcggttc gtcccggcgg tcatgtctgg acgactcggt tcgatgcgca tcccggcctc
     2941 tcggcgtcgg tacgttgacg agggtatccc aacaccatgt tcatacatcc cgtatcggta
     3001 acaggggcgt tttcatgaag gaccgtgctg tgcggcacct acccggaatc cgtaggccac
     3061 cgcagaggcg tgccgagatg cccacccggg ttcgcggccg agaaagcggc aggctcgggg
     3121 ggcgccccga tgccacgcgg tggtccccga gggtggtcac cccgaaagcc ctgcgctgca
     3181 aggtcgacgg gcggccggtc cccttggcga cacccgatcc ttgcgctctg tgacggcgaa
     3241 accacttcgg gctctgttcg tccagatgca agggccgtgg ttcccccctg ccgggcggag
     3301 aggcacctgc ctccatccag gcgtgcaccc aggcggcgaa acgctgccgg agcggctcac
     3361 gcctgttccg ccgtcgcctc ggcggtcccc ttccatgccc tcaggaccgc atgcatgagg
     3421 gtggcgagcg ggatggcgaa caccaggccc cagaagcccc acaggccgcc gcagacgagg
     3481 atcgccacga tgatcgcgac ggggtggatc ctcaccaccc gcgacaggag caggggtgcg
     3541 acgaggttcc cgtcgagggc gtggagcgcg aggtacgcga tcaccgtcca cgtcgtgtgc
     3601 gcgcccaagc cccactggaa gaaggccacg aggacgacgg gcacgaacat gaccgccgcg
     3661 ccgatgtagg gcaccagcac ggccaaaccc acgagcatcg agaggatgac cgagtacttg
     3721 aggcccagcg cggcgaaggc cgcgtaattg atcgcccaca cgaacatgat ctcgaggacc
     3781 ttgcccctga ggtagttgaa gaactggtcg tagacctcgt cccacacgag tacggccagg
     3841 ccgcgttcct ccggtatgaa gccggacagc cagtccagga tgagcttctt gtccttgagc
     3901 atgaagaaga ccaagaacgg cacgagcacg aggtagacga cgatctccac cacgcccttg
     3961 gccgagacga ccgacagctt cacgatgccg ccgcctatcc cttcgatctc ctcgctcagg
     4021 atcgcgagcg ccctgtcgac cgtctcctcg gtgatcaccc ccgggtattt ctggggcagg
     4081 gtcgacaccg tggccttgat cgtcgagacc atgcccggca ggtccctgat gagttcggac
     4141 acctgtctcc agacgagcgg cagcacgatg aggaggagat ccacgaacac gaacacgaac
     4201 aggaggaaga ccaggtacac gagggtctcc ctcctgacgt tgaagcgcat gagccaggtg
     4261 atgaggctgt ccaggaggac ggagagcacg acggccacga gcaccggtgc gagcatcctc
     4321 gagaacaggg agaagacgac gaagcctgcg acgaggatca ggatcaggat caggacctgg
     4381 gggtccgaga agtagcgcct gagccacctt ctgaggacgt gtttcatcgc ttcgaggtct
     4441 gggtgcaggt cggggggagg ggggtaagga agggcccgct cccttttgtg gggcgggccc
     4501 ggacgttgtc gtcaggcatc acttgcccag catgttctcc ttgagggagt cctgagcggg
     4561 ctccttgccc agccagatgc cgaagagcgc cttcttgaag tcgaggccct ggatgaccga
     4621 gacgagcttg ccggccttca tgatctcgat gcccttgccg ggctggtaga cgaagtcgta
     4681 gacgtcgtcc ttggcgaggc ccctgaagca cgcggtgaag gcgtcgatct tcgccttcag
     4741 cggggccacg ttgccgcccg tggacttctc gaacccttcg tcgatggccg ccttcatctt
     4801 gtcgggggtc acgaggccgg acgttatctg gagcctgatg gccatgggct cgtcggcgtt
     4861 gatgatggcg ttggcatcag cgctcttctg cttgaggtag agggccgcca cgtagacctt
     4921 catcccgaac ttggtcctct tcccaccgcc gttgaggaca aggctggtat cccctgcctt
     4981 gagggtgtcg ggcatgtcga cacccacgat gtcgaccgcg aacgccgatg tcgccaggaa
     5041 gacaagggcc acagcgagaa aacctgctct cttgagaacc atagtaccct cctctctatg
     5101 ttatggtgtg aagaaagttg aaggatgtca cccgaacgat actaccctct cccatccccc
     5161 gaatcaagac ggaaaagagg ggcgtctcct gcaggcgggg gaagctcgtc gcgaggatca
     5221 ggggcttttg gggccgaggc ccagttcctt gtagatcctc agcgtggcgc gggcgttgtt
     5281 gagggtatag aagtgcacgc ccttgacgcc gtgggagacg aggtccctca cctggtcgat
     5341 ggcccacgtc actcccgcgt gttccaccgc ttcaggcgac ggggccgagt acaccgcgcg
     5401 caaaagcggg gccgggaacc tcgcgtgggc cgccagttcg gccatgcgct ccatggagct
     5461 cacgctcacg atgggcatga cgcccgccac gatgggcacg tggatgcccg caagctcgca
     5521 cctctcgcag aagtcataga agtccctgtt gtcgaagaag agctgggtga tgatgtagtc
     5581 tgccccggca tcgaccttgg ccttgaggta ggctatctcc ctgagcctgt tcggcgtctc
     5641 gggatgcccc tcggggaacc cggccacccc cacgcacatg ccggggaagc gcctcttgat
     5701 gaagcccacc aggtcgatcg cgtgtgcgaa cccgccaggc gcggggtccc accgggcgtg
     5761 gcccgcggga gggtcccccc tcaaggcgag gatgttctcg atgccgcttg catggtagcg
     5821 ctcgaggatg gccatgatgt ccttgtccgt ggactcgacg caggtcaggt gagagaccac
     5881 ggtgaggtcg gtctggctgt tcaggcgtac cacgaggcgg tgggtgttct ccctcgtaga
     5941 gcccccg
//
LOCUS       DAOSCR010000080         5930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8517_length_5930_cov_3.912681, whole genome shotgun sequence.
ACCESSION   DAOSCR010000080 DAOSCR010000000
VERSION     DAOSCR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8517_length_5930_cov_3.912681"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLT69_03290"
     CDS             complement(<1..737)
                     /locus_tag="PLT69_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOJ13497.1"
                     /translation="MNLGYIDYLNCYPFYHHMFEYEPVSGVRIVPGYPSELNRMIQER
                     SLDVSPISAAAYAGLEDELVLIPEFCLSSIGYVRSVVLVSRMPIEDLQGRVIGLSSAS
                     KTSVVLLKILLQRFYGIDAVYVPSGTSPSLDREGYDAALIIGNEAMRQLRDPYVYDLG
                     DLWFRKTGYPVVFAVFAIRSEIIETHEKEILEVVASYRRSLDCLRTDRTGLIQKARER
                     YPLVSWDIDAYYRLLQFRFTPGLREGLG"
     gene            complement(734..>998)
                     /locus_tag="PLT69_03295"
     CDS             complement(734..>998)
                     /locus_tag="PLT69_03295"
                     /inference="COORDINATES: protein motif:HMM:NF039584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOJ13498.1"
                     /translation="FAAARVFLHNVPHLKALWMYLGEKMAQTLLCFGADDLGGTYHHE
                     RVVHAAGAPTADSGSEVTLIRLIENAGMTPVRCAADYRERRAS"
     assembly_gap    999..1008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1009..1836)
                     /locus_tag="PLT69_03300"
     CDS             complement(<1009..1836)
                     /locus_tag="PLT69_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13499.1"
                     /translation="MDQALNLETIRNKTLRDIARKVEACLPVSEDDALFMLTTNDILE
                     LGLIASYVKRRLHGDRVFYSVNMNLNYTNVCELRCPICAFSRSEGEDGAYVLDLDEIE
                     HRARWAASMGVDEIHIVGGLNRSLKLDYFEEMLRRIKRTCPDALIVAFTAVEYDFFAK
                     ENGIPLDEVFSRLIAAGLGALPGGGAEIFAPRIREVIAPRKISGRRWCEVMRQAHTLG
                     LKTNATLLYNHLERPEDIVDHLASIRSLQDETGGFKTFVPLPYHETGSLVRAKAKTST
                     "
     gene            complement(1961..3217)
                     /locus_tag="PLT69_03305"
     CDS             complement(1961..3217)
                     /locus_tag="PLT69_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOJ13500.1"
                     /translation="MDFGAVYDGEGKIYVMGGNVFDVMPHRHSRCYREGMYSTLVYDI
                     RGDRWEYLEPVPGMAALDSTFMYYDEAGKEWRYGSKKQVVNRETGELSYEMPEEYRWL
                     GDRARNSDMSRFGAGVRIARLKDGRILWTGGRRHVGNSERVVLSFDPSQGRWCRVKAR
                     RIYESYGWRLTSEYDPPVAWMQERRILHEAVTTSDGKVYVMGGWRVETKGGDKDYIIS
                     TREEPTYINDSVECYDPGTNTWTYRSPMRSPRFFFAAVVLGDDRILVFGGETADERHY
                     RNFKVLDVVEEYDPRQDRWREVGRMPEARKGHAAALGSDGKVYITGGARIREGRIENT
                     TWVYDPVTGGWERGPGMRRARHGHRMVAVPGRVCAIGGTDYEGEGFRRLANEYFLPRW
                     FQVYEGKVQETVEVLEVDVSPGPRGG"
     gene            complement(3570..4826)
                     /locus_tag="PLT69_03310"
     CDS             complement(3570..4826)
                     /locus_tag="PLT69_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOJ13501.1"
                     /translation="MDFGAVYDGEGKIYVMGGNVFDVMPHRHSRCYREGMYSTLVYDI
                     RGDRWEYLEPVPGMAALDSTFMYYDEAGKEWRYGSKKQVVNRETGELSYEMPEEYRWL
                     GDRARNSDMSRFGAGVRIARLKDGRILWTGGRRHVGNSERVVLSFDPSQGRWCRVKAR
                     RIYESYGWRLTSEYDPPVAWMQERRILHEAVTTSDGKVYVMGGWRVETKGGDKDYIIS
                     TREEPTYINDSVECYDPGTNTWTYRSPMRSPRFFFAAVVLGDDRILVFGGETADERHY
                     RNFKVLDVVEEYDPRQDRWREVGRMPEARKGHAAALGSDGKVYITGGARIREGRIENT
                     TWVYDPVTGGWERGPGMRRARHGHRMVAVPGRVCAIGGTDYEGEGFRRLANEYFLPRW
                     FQVYEGKVQETVEVLEVDVSPGPRGG"
     gene            complement(4931..>5930)
                     /locus_tag="PLT69_03315"
     CDS             complement(4931..>5930)
                     /locus_tag="PLT69_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13502.1"
                     /translation="SMGGLISRSYQARAGKARSMLIVGTPNHGTYGVINHVLSIPDLA
                     IPLFEVLSFVCGSPVCLDLLSPGTADLMYYDDTAPEATSGNPSLYALNQNAVSKGKAK
                     TTLIAGTDSSAYLYLPGLALPEPNDSVVPADSVFCLSSTAPARAKAYSLLDYSGLTIN
                     DCIYHDDTCRNETYHFNHFNFGTNEFSVEKIINDIRKGLSDWTVGKRRFFSWNDERAG
                     TEGMAKATMVVNYNTTGKDIDRVVLVLYARDTSGAWHIYDQGQNGADAEGNVNANKVK
                     PISGVSVDEVVLDVSCILPDPDTVEEVMPCLYSLRPGRTKVPLVPEPDFSMPVEER"
BASE COUNT         1035 a         2194 c         1485 g         1206 t
ORIGIN      
        1 ccgagcccct ccctgagtcc gggcgtgaac ctgaactgga gcaggcggta gtaggcgtcg
       61 atatcccatg acacgagggg ataccgctcc ctcgccttct gtatgaggcc ggtcctgtcg
      121 gttcgcaggc agtcgagcga tctcctgtac gatgcgacga cctcgagaat ctctttctcg
      181 tgtgtctcga tgatctcgct gcggatcgcg aacaccgcga agaccaccgg gtagcccgtc
      241 ttcctgaacc acaggtcgcc caggtcgtag acgtacgggt ccctcagctg tctcatggcc
      301 tcgtttccta tgatgagggc cgcgtcgtac ccttcccggt cgagtgacgg ggacgtcccg
      361 gagggaacgt atacggcatc gatcccatag aacctctgga gaaggatctt gaggagaacc
      421 accgaggtct tggaagcact ggagagcccg atgaccctgc cctgcaggtc ttctatgggc
      481 atccggctca cgagcaccac ggacctgacg tacccgatgg agctcaggca gaactcggga
      541 atgagcacga gctcgtcctc gaggcccgcg tacgcggccg ctgagatcgg gctgacgtcg
      601 agagaccgct cttggatcat ccggttcagt tcgctcgggt acccgggcac gatcctgacc
      661 ccgctcacgg gctcgtactc gaacatgtgg tggtagaacg gatagcagtt caggtagtct
      721 atgtatccga ggttcatgat gcacgccttt ccctgtagtc tgcagcgcaa cgcaccgggg
      781 tcatgcccgc gttctcgatg agccttatga gcgtcacctc gctgccgctg tccgccgtgg
      841 gtgcgcccgc agcgtgcacg accctttcgt gatggtacgt cccgcccagg tcgtccgcgc
      901 cgaagcacag aagcgtctgt gccatctttt ctcccaggta catccacaat gccttgaggt
      961 gcgggacgtt gtgcaggaag acgcgtgcgg cagcgaacnn nnnnnnnngg tggaggtctt
     1021 tgccttggcc ctcaccaggg agcccgtctc gtggtagggg agcggcacga aggtcttgaa
     1081 cccgcctgtc tcgtcctgga gcgaacgtat cgaggcgagg tggtccacga tgtcctcggg
     1141 cctttccagg tggttgtaga ggagggtggc gttcgtcttc aggcccaggg tgtgagcctg
     1201 gcgcatgacc tcgcaccacc tccggcccga gatcttcctc ggcgcgatca cctcccttat
     1261 cctcggcgcg aagatttcgg cacccccgcc gggcagcgca ccgagcccgg ctgcaatgag
     1321 gcggctgaag acctcgtcga gggggatgcc gttttctttc gcgaaaaagt cgtattccac
     1381 tgcggtgaag gcgacgatga gggcgtcggg acaggttctc ttgatcctgc gcagcatctc
     1441 ctcgaaatag tccaacttga gcgacctgtt gagcccgccc acgatgtgta tctcatctac
     1501 acccatggag gcggcccacc tcgccctgtg ctctatctcg tccaagtcaa gcacgtacgc
     1561 cccgtcttct ccctcggatc tcgaaaaggc gcagatcggg caacgcagtt cgcagacatt
     1621 cgtgtagttg aggttcatgt tcacggagta gaacaccctg tcgccgtgaa ggcgtctctt
     1681 gacgtacgag gcgatcaagc cgagctcgag gatgtcattg gtcgtgagca tgaagagggc
     1741 gtcgtcctcg ctcacaggaa ggcacgcctc gacctttcgc gctatgtctc tcagggtttt
     1801 gttccggatg gtctcgaggt tgagggcctg gtccaagatc gtctctggct ggagttttct
     1861 ctgttgcagt agtgttttat gctagccggt ctcgctcccg gctgtcaacc ggcttgtccc
     1921 gttcggttct tcttcctctc catcccctgt cccgtgaggc tcacccgccc ctgggcccgg
     1981 gggagacgtc cacctcgagc acctcaaccg tctcctgcac cttgccctcg tagacctgga
     2041 accacctcgg cagaaaatac tcgttcgcca gcctcctgaa cccctcgccc tcgtaatccg
     2101 taccccctat cgcgcacacc ctcccgggca cggccaccat ccggtgccca tgacgcgccc
     2161 gcctcatccc tggccccctc tcccaccccc cggtcacggg atcgtacacc cacgtcgtgt
     2221 tctctatcct cccctcacgt atcctcgccc cgcccgtgat atacaccttc ccgtccgacc
     2281 ccagcgccgc agcatgaccc ttcctcgcct cgggcatcct ccctacctcc ctccaccggt
     2341 cctgcctcgg gtcgtactcc tccaccacgt ccaatacctt gaagttccga taatgcctct
     2401 cgtcagccgt ctcaccacca aacaccagta tccggtcgtc ccccagcacc accgcggcga
     2461 aaaagaaccg cggactcctc atcgggctcc ggtacgtcca cgtgttcgtc ccaggatcgt
     2521 aacactccac cgaatcgttg atgtacgtcg gctcctcccg cgtcgatatg atgtaatcct
     2581 tgtcccctcc cttcgtctcc acccgccaac cccccatcac gtacaccttc ccgtctgacg
     2641 tcgtcaccgc ctcgtgcagt atcctcctct cctgcatcca cgcaaccgga ggatcgtact
     2701 ccgacgtaag tctccaccca tagctctcat aaatcctcct tgccttcacc ctgcaccacc
     2761 gcccctggga agggtcgaag ctcaacacca cgcgctcgct gttgcccacg tgcctccggc
     2821 ctccggtcca caggatcctc ccgtccttca gcctcgctat ccgtacccct gccccaaacc
     2881 gcgacatgtc cgaattcctc gcccgatccc ccaaccaccg gtactcctcg ggcatctcgt
     2941 agctcagctc ccctgtctca cggttcacca cctgcttctt cgacccgtac ctccactcct
     3001 tccccgcctc gtcgtagtac atgaacgtgc tgtccaacgc cgccatacca ggcacgggct
     3061 ccaggtactc ccaccgatcc ccccgaatgt cgtacacgag cgtcgagtac ataccctcac
     3121 ggtaacacct tgaatgccgg tgaggcatca catcgaacac gttgccgccc atcacgtaga
     3181 tcttcccctc gccgtcgtac accgccccaa agtccatcct cccgtgcggc atcggcgtcg
     3241 cgtaccgcca ccccgctccc acggttccgg gcatcaccac cccaaccacc acccacacga
     3301 gaacccatcc ccttgctcgc atcaccgctc ctccaccggc atgctgaaat ccggctccgg
     3361 caccaacggc accctcgccc tcccaggcct caagctgtac aggcacggca tcaccgtgca
     3421 ggctcgatcc ttgtccctaa tggcgggcac tgataagatg atctcctgaa ggtcaaccca
     3481 ggtcaggact ttggacaatc gtcaccttgt tccgttccct cgccaaatga catacaaaac
     3541 atgcatcgtc ggccatgtac cgctgaggct cacccacccc tgggcccggg ggagacgtcc
     3601 acctcgagca cctcaaccgt ctcctgcacc ttgccctcgt agacctggaa ccacctcggc
     3661 agaaaatact cgttcgccag cctcctgaac ccctcgccct cgtaatccgt accccctatc
     3721 gcgcacaccc tcccgggcac ggccaccatc cggtgcccat gacgcgcccg cctcatccct
     3781 ggccccctct cccacccccc ggtcacggga tcgtacaccc acgtcgtgtt ctctatcctc
     3841 ccctcacgta tcctcgcccc gcccgtgata tacaccttcc cgtccgaccc cagcgccgca
     3901 gcatgaccct tcctcgcctc gggcatcctc cctacctccc tccaccggtc ctgcctcggg
     3961 tcgtactcct ccaccacgtc caataccttg aagttccgat aatgcctctc gtcagccgtc
     4021 tcaccaccaa acaccagtat ccggtcgtcc cccagcacca ccgcggcgaa aaagaaccgc
     4081 ggactcctca tcgggctccg gtacgtccac gtgttcgtcc caggatcgta acactccacc
     4141 gaatcgttga tgtacgtcgg ctcctcccgc gtcgatatga tgtaatcctt gtcccctccc
     4201 ttcgtctcca cccgccaacc ccccatcacg tacaccttcc cgtctgacgt cgtcaccgcc
     4261 tcgtgcagta tcctcctctc ctgcatccac gcaaccggag gatcgtactc cgacgtaagt
     4321 ctccacccat agctctcata aatcctcctt gccttcaccc tgcaccaccg cccctgggaa
     4381 gggtcgaagc tcaacaccac gcgctcgctg ttgcccacgt gcctccggcc tccggtccac
     4441 aggatcctcc cgtccttcag cctcgctatc cgtacccctg ccccaaaccg cgacatgtcc
     4501 gaattcctcg cccgatcccc caaccaccgg tactcctcgg gcatctcgta gctcagctcc
     4561 cctgtctcac ggttcaccac ctgcttcttc gacccgtacc tccactcctt ccccgcctcg
     4621 tcgtagtaca tgaacgtgct gtccaacgcc gccataccag gcacgggctc caggtactcc
     4681 caccgatccc cccgaatgtc gtacacgagc gtcgagtaca taccctcacg gtaacacctt
     4741 gaatgccggt gaggcatcac atcgaacacg ttgccgccca tcacgtagat cttcccctcg
     4801 ccgtcgtaca ccgccccaaa gtccatcctc ccgtgcggca tcggcgtcgc gtaccgccac
     4861 cccgctccca cggttccggg catcaccacc ccaaccacca cccacacgag aacccatccc
     4921 cttgctccca tcaccgctcc tccaccggca tgctgaaatc cggctccggc accaacggca
     4981 ccttcgtcct cccaggcctc aagctgtaca ggcacggcat cacctcctcc acggtatcgg
     5041 ggtctgggag aatgcatgat acatcaagaa ccacttcgtc tacggaaaca cctgaaatcg
     5101 gcttcacttt attcgcattg acattgccct ccgcgtctgc gccgttttgg ccttggtcgt
     5161 agatgtgcca tgcacccgag gtatccctgg cgtagaggac gagcaccacc cggtcgatgt
     5221 ccttgccggt ggtgttgtag ttcaccacca tggtcgcctt ggccatgccc tcggtgcccg
     5281 cccgctcgtc gttccaggag aagaaccgcc tctttcccac ggtccagtcg gagagcccct
     5341 ttcgtatgtc gttgatgatc ttctctaccg agaactcgtt cgtcccgaag ttgaagtggt
     5401 tgaagtgata ggtctcgttt ctgcacgtgt cgtcgtggta gatgcagtcg ttgatcgtga
     5461 ggccgctgta atcgagcagc gagtacgcct tcgccctggc aggcgcggtg gatgagaggc
     5521 agaacacgct gtccgcgggg acgaccgagt cgttgggctc gggcagggcg aggccgggca
     5581 ggtagaggta cgccgacgag tccgtcccgg cgatgagcgt cgtctttgcc ttgcccttgc
     5641 tcacggcgtt ttggttcagg gcatatagcg aggggttccc gctcgtcgcc tcgggcgccg
     5701 tgtcgtcata gtacatgagg tccgcagtgc cgggcgagag caggtccagg cagacgggcg
     5761 acccgcacac gaaggagagc acctcgaaca aggggatcgc caggtcgggt atggacagga
     5821 cgtggttgat cacgccgtag gtcccgtggt tgggcgtacc cacgatgagc atgctgcgcg
     5881 ccttgccggc gcgggcctgg taggaccggg atatgagccc tcccatgctg
//
LOCUS       DAOSCR010000081         5911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8546_length_5911_cov_3.942794, whole genome shotgun sequence.
ACCESSION   DAOSCR010000081 DAOSCR010000000
VERSION     DAOSCR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_5911_cov_3.942794"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLT69_03320"
     CDS             <1..320
                     /locus_tag="PLT69_03320"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOJ13503.1"
                     /translation="GATRVLIASHTGDHPIYPDCTPDFTEAMSRAAFYGTFSGVRVEA
                     PFSGYDKRRIAQTGRSLGFDFTMTWSCYRGGDLHCGTCPTCLERKEALGYNEGLDPTR
                     YLA"
     gene            317..1117
                     /locus_tag="PLT69_03325"
     CDS             317..1117
                     /locus_tag="PLT69_03325"
                     /inference="COORDINATES: protein motif:HMM:NF026169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HOJ13504.1"
                     /translation="MNGIPLGRKVRPPERYDPSVLFRIERRKVEGNIFGYDLWRCYEM
                     SWLNERGRPHAGILEIAYPSTSAFIVESKSLKLYLAGISCERFPSKDTLVRVIASDIE
                     RTVEAQWIQVRIEGPEGFGSFMPVPEIPAESLDSLDIAVERSGPDAGLITCTDGYVEE
                     ALCTDLLKTVCPITGQPDWASVLVRYRGRPMDREGLLHYLCSYRGHAGFSEDVCTLIY
                     TDIARRCRPDELGVTCFYTRRGGIDITARRQDRPASPGDVERIRLVRQ"
     gene            complement(1230..1703)
                     /locus_tag="PLT69_03330"
     CDS             complement(1230..1703)
                     /locus_tag="PLT69_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13505.1"
                     /translation="MKRAGFLAVLCTAGLLMLPCAASAQLVQLADEQLGEVVGQAGIA
                     FDQHYDNVSGMGGLLNYSDVTLVGSVEVRNQTEVPQDLVSQMAMPGFSMFGLGLMGLR
                     SMGIGTHAIDMTINIDQLTIGAIRVGNDTTGPELGSLSIYGLRADIKGTVTIWND"
     regulatory      complement(1730..1815)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(1912..1997)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2371..5586)
                     /locus_tag="PLT69_03335"
     CDS             complement(2371..5586)
                     /locus_tag="PLT69_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13506.1"
                     /translation="MGDVSFLGYAAAPSSKDLDSSGQARSPGKTGGARRKRFVTVLCT
                     DLSGYTRLSSLLDGEDLAACMARVMDTVAGAVRMFGGEVEKYVGDAVIALFGMERTGE
                     HDPLRAVEAARRIHHEVERLPVDELPGLPGGLCMHTGICSGEVIVDEAARTSGRHGAL
                     GLPITAACRLCDIARAGEILVGGAVTQAVSRFFRLEWLGLRSLEGISSPMNVFRVIEY
                     RPERVSLHRETGLVSNMVGRKRELEALIEWAADAPRGRGGVVCVSGEAGIGKSRLVRE
                     LEKRMGGTLLFVSSSCHEHTRSIPYHPLRDLVFRTMTAVREKGLLAGAGCARRYDLID
                     RLYRDAMNTLGFAVEAREANYGAARERACDVLLRFLDEFSGAAPAVFCIEDIHWADQS
                     TLDFLAFAVDAFSRGCACLLVLTLRGGFECHAAARSVELKELGVKEVEAMLTSMTGGM
                     EVGDDVVRWLLNASGGNPFFVEEIVNYLMERGVDLAGCESSPVLTDAPATIMGLIASR
                     MDGLDPGPRRVLGEAALLGRMFSRDVLRAVTGEPLVLDACLEALKDHGFHSSEDGETF
                     SFRHDIIRDAASRTMMKHERTSVHKRIVHVLETSPPGGLDNLDDMLARHSLEASLFDR
                     AAGYHLKAARTCLEKGAWFEAATLYGAAERILEEHPEVPGSGELLEASREGLWMCCRV
                     FDPARATRALEALKRRYRSLGRTRDEAFTLARLVNLYSQVCAFDKAEEVYREALTLCG
                     ADNWLASAVKTVYAYTCTYLGRPLDALRLLAEARGVLKEAGGFLYAVNALTTLAASVW
                     KGDTVEASSWYEETKKSCGDHLDIDIMADLWLYHVLCLGGRFNEAMAVHEEARRRELK
                     LGRLAGGLSYLRIQGSIYFRSRYLGDLAGAREDLARFQPMARSIQNGRALDMLYRAWI
                     ALEEGDAGTARRLAASALPDLSRGVANRVPYALNTLAEAMIVEGDARGAGDVLDECIE
                     RTGRYGNMEQHLWAMRLKGCVLVRTGDLEGGASVLRRAAALARDARLTPQLAWVLAAM
                     AELERAKGRAHRARRLCEASGRLWARLGNGYQASKALSSVT"
     gene            5728..>5911
                     /locus_tag="PLT69_03340"
     CDS             5728..>5911
                     /locus_tag="PLT69_03340"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852309.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HOJ13507.1"
                     /translation="MVMKLKKIWFDGSLVDWDDATVHVLTHTLHYGLAAFEGIRCYRC
                     HDGRSAIFRHREHTRRL"
BASE COUNT         1042 a         2026 c         1822 g         1021 t
ORIGIN      
        1 tcggggcgac cagggtgctc atcgcgtccc ataccggcga ccaccccata taccccgact
       61 gcacccctga cttcaccgag gccatgtccc gcgccgcctt ctacgggacg ttttccggcg
      121 tgagggtcga ggcccccttc tcggggtacg acaaacggcg catcgcccag acgggcaggt
      181 cgctcggctt cgacttcacc atgacctggt cgtgctacag gggcggagac ctccactgcg
      241 gcacctgccc cacctgcctg gaacgcaagg aggccctggg gtacaacgag ggcctcgacc
      301 ccacgaggta cctcgcgtga atggcatccc cctgggcagg aaggtccgtc cgcccgagcg
      361 ctacgaccct tcggtcctgt tccgcataga gaggaggaag gtcgagggga acatcttcgg
      421 ctacgacctc tggcgatgct acgagatgtc ctggctcaac gagagaggca ggccccatgc
      481 ggggatcctc gagatcgcat acccgtccac gagcgccttc atcgtggaat cgaagtccct
      541 caagctctac ctcgcaggca tttcgtgcga gcgcttcccg tcgaaagaca cgctcgtgcg
      601 cgtcatcgcc tccgacatcg agcgcaccgt ggaggcgcaa tggatccagg tgaggataga
      661 gggaccggag gggttcggct cgttcatgcc cgtacccgag atcccggcgg agtccctcga
      721 ctcgctcgac atcgcggtcg aacgttcagg tcccgacgcg ggactcataa cgtgcaccga
      781 cggctacgtg gaggaggcgc tctgtacgga tctgctgaag accgtctgcc ccatcacggg
      841 acagccggac tgggcgagcg tgctcgtgag gtaccgaggg cgcccgatgg accgggaggg
      901 cctgctgcac tacctgtgtt cctatcgggg ccacgccggg ttctccgaag acgtctgcac
      961 gctcatatac acggacatag cacggaggtg ccggccggac gagctcggcg tcacctgctt
     1021 ctacaccagg agagggggga tcgacataac cgcccgcagg caagacaggc ccgcttctcc
     1081 cggcgacgtg gaaaggatca ggctcgtgcg ccagtgaccc tcacttcccc gtgggcctcc
     1141 ccgcgcaaga tcgctcactg tctccggacg aaggcagccg gctcgagggt gtcactcgaa
     1201 aagggggtaa aagagtaacc ccgtccgttc tagtcgttcc atatggtgac cgttcccttg
     1261 atatccgccc gcaggccgta tatggagagg ctgccgagct ccggcccggt ggtgtcgttg
     1321 ccgaccctga tggcgccgat ggtgagctgg tcgatgttga tggtcatgtc gatggcgtgg
     1381 gtgcctatac ccatggacct gagccccatg aggccgagac cgaacatcga gaacccgggc
     1441 atggccatct ggctgacgag gtcctggggc acctccgtct ggtttctcac ctccaccgac
     1501 cccacgaggg tcacgtcgct gtagttcaga aggcccccca tgccggacac gttgtcgtag
     1561 tgctggtcga aggcgatacc cgcctggccc acgacctctc ccaactgctc gtcggccaac
     1621 tggaccagct gcgccgatgc ggcgcatggg agcatgagca gccctgccgt acagaggact
     1681 gcgaggaatc ccgctctttt catgaaaaac tcctttctga tgaggttctc caggcaaccc
     1741 ggccgtccga cccctgcggg gtacggaccc gtggctttcc ggcccagggt ctcccctggt
     1801 gtggcttgag tgactgatct gcagaatcct tctcaggcag ctcggctgtc cgggcactct
     1861 ctccggaccc gtggctttcc ggcccagggt ttcccctggt gtggctttga aggaggcaac
     1921 ccggctgtcc gcgccgcacc cggcacggac ccgtggcttt ccgtcccacg gttacccgtg
     1981 gtttggcatg gcagggacct ctccaaggcg caccacaggc ggcgcaccgt ctcgagaccc
     2041 cttgcgttgt ctgtcgggaa aacgaacgtt tacgagtgtt actcttttac ccgtgtgcgg
     2101 tctctggtgc caaagagcgc acacggctca aacccctcgg ttgtcaaaga gcgcacggcc
     2161 ttgaaggcct tctctgaatc acctatcgat cggaaacgag aaaacatgag gggcatgcac
     2221 atgaggggcg cccgcatgcc caacgccctg catacagacc ctcgagacgc gggttctgtc
     2281 ttgcatggtc gcgggtccag gcggtcttaa ggattacgtt tgtgtaatat cgcccggcag
     2341 ggaacgggcc aggccgcggc gctctccccc tcaggtgacg gagctgagcg ccttcgacgc
     2401 ctgatagccg tttccgagcc ttgcccagag acgccctgag gcctcgcaca ggcgcctggc
     2461 tcggtgtgca cggcccttgg cgcgctcgag ttcggccatg gccgcgagca cccacgccag
     2521 ctgcggggtg aggcgggcgt ccctggcgag cgccgcggca cggcggagaa cggacgctcc
     2581 cccttcaagg tcacccgtac gaacgagcac gcaccctttg agcctcatcg cccacaggtg
     2641 ctgctccatg ttgccgtaac ggccggtacg ctcgatgcac tcatcgagga cgtcgcccgc
     2701 tccccgcgca tcgccctcca cgatcatggc ctccgccaat gtgttgagag cgtaggggac
     2761 cctgttggcc acaccccggg agagatcggg cagggccgat gcggcgagac ggcgcgcggt
     2821 tccggcgtcc ccttcctcga gggcgatcca cgcccggtaa agcatgtcga gggcgcggcc
     2881 attctggatg gatcgagcca tgggctggaa ccgtgcgagg tcttcccgcg cgccggcgag
     2941 atctcccagg tagcggctcc tgaagtagat ggaaccctgt atcctcaggt acgagagccc
     3001 gccggcgagc ctgccgagct tgagctctcg acgcctcgcc tcctcgtgga cggccatggc
     3061 ctcattgaag cgccctccta ggcacagcac gtggtagagc catagatcag ccatgatatc
     3121 gatatcgagg tgatcgccgc agctcttctt cgtctcctcg taccatgacg aggcctcgac
     3181 ggtgtccccc ttccacacgc tcgccgccaa cgtggtgagg gcgttgaccg cgtagaggaa
     3241 gccgcccgcc tccttcagga ctccccgcgc ctccgcgagc aggcgcagcg catcgagcgg
     3301 tctcccgaga taggtgcagg tgtaggcgta gacggtcttg accgcactgg ccagccaatt
     3361 gtcggcgccg cacagggtga gcgcctcgcg gtagacttcc tcggccttgt cgaaggcgca
     3421 cacctgcgag tacaggttga ccagcctcgc cagcgtgaac gcctcgtccc tggtacgccc
     3481 cagggatcgg taacgcctct tgagcgcctc gagggccctc gtcgccctgg ccgggtcgaa
     3541 caccctgcag cacatccaca ggccttccct cgaagcctcc aggagttcac cggagccggg
     3601 cacctccggg tgctcctcga ggattcgttc cgcagccccg tacaaggtcg cggcctcgaa
     3661 ccacgccccc ttctcgaggc acgtccgcgc cgctttgagg tgatacccgg cggccctgtc
     3721 gaagagcgac gcctccaggc tgtggcgggc gagcatgtcg tcgaggttgt cgagcccccc
     3781 gggaggcgat gtctcgagga catgaacgat cctcttgtgc accgaggtcc tctcgtgctt
     3841 catcatcgtc ctcgacgcgg cgtccctgat gatgtcgtgc ctgaacgaaa aggtctcgcc
     3901 gtcctcgctc gagtggaaac cgtgatcctt gagcgcctcg aggcaggcgt ccaggacgag
     3961 aggttccccg gtgaccgcac ggaggacgtc cctggagaac atcctgccca ggagcgcggc
     4021 ttccccgagg accctgcggg gcccggggtc gagaccatcc atgcgtgagg cgatgaggcc
     4081 catgatcgtg gcgggtgcgt ccgtcaagac gggagagctc tcgcacccgg cgagatccac
     4141 acccctctcc atgagatagt tcacgatctc ctcgacgaag aacgggttgc cgccggatgc
     4201 gttcaggagc cacctcacca cgtcgtcccc gacctccatg cccccggtca tggacgtgag
     4261 catggcttcg acctccttga cgccgagttc cttgagctcc acggaccggg ccgccgcatg
     4321 gcactcgaat ccgcccctga gcgtgagcac gagcaggcag gcgcaccctc tcgaaaacgc
     4381 gtcgacggcg aacgccagga aatcgagggt gctctggtcg gcccagtgga tgtcctcgat
     4441 gcagaagacg gcgggcgcgg caccggagaa ctcgtcgaga aacctcaaga ggacatcgca
     4501 ggcgcgttcc ctggcggcgc cgtagttggc ctcccttgcc tccacggcga aaccgagggt
     4561 gttcatggcg tcacggtaga ggcggtctat gaggtcgtac ctccgggcac aaccggcgcc
     4621 cgcaaggagc cccttttcgc gaacggccgt catggtgcga aagacgaggt ccctgagcgg
     4681 gtggtagggg atggaccggg tgtgctcgtg gcacgaggac gagacgaaca ggagcgtgcc
     4741 ccccatcctc ttttcgagct ccctgaccag cctgctcttc ccgatgcccg cttccccgga
     4801 gacacagacg acaccccccc tgcccctcgg cgcatctgcg gcccactcga tcagcgcctc
     4861 gagctccctc tttctcccca ccatgttcga aaccaggcct gtctcgcggt ggagggacac
     4921 ccgctcgggg cgatactcga tcacgcggaa cacgttcatc ggggaagaga tgccctcgag
     4981 actcctcaag cccaaccact ccaggcggaa gaacctcgag acggcctggg tcaccgcacc
     5041 cccgacaagg atctctcccg cccgggcgat gtcgcagaga cggcatgccg ccgtgatggg
     5101 caagcccaag gccccgtgcc ttcccgacgt ccgtgcggcc tcatccacga tcacctcgcc
     5161 ggagcatatc cccgtgtgca tacagagccc accgggaagc cccggaagct cgtcgaccgg
     5221 gaggcgctcc acctcgtgat gtatgcgcct ggccgcctcg acggccctca gggggtcgtg
     5281 ctcacccgtg cgctccatgc cgaagagggc gatcaccgca tcgcccacgt acttctccac
     5341 ctcgcccccg aacatcctga ccgcaccggc gaccgtgtcc atgacgcgcg ccatgcacgc
     5401 cgcaaggtct tcgccgtcga ggagagagct cagcctggta tacccgctca ggtccgtgca
     5461 gagcaccgtc acgaaacgct ttcgcctcgc gccccccgtc ttgccgggag agcgggcttg
     5521 cccggacgag tcaaggtctt tcgaagaagg cgcggccgca tacccgagaa atgagacatc
     5581 acccataacc cgacctccca aagaaagatc atagacgatc atccccgcac ccgtcgggcg
     5641 gcgttcaccc ggcaaacttc gtgttgaccg tgctcgaggt ttcgtcgata atgcatggaa
     5701 tataccctaa tttgaggtgc ccttgctatg gtgatgaaac tcaagaagat ctggttcgac
     5761 ggctctttgg tggactggga cgacgccacg gtccacgtgc tgacccacac cctccactac
     5821 ggtctcgccg ccttcgaggg gatccggtgc tacaggtgcc acgacgggcg ctcggcgatc
     5881 ttcaggcaca gggaacacac caggaggctc t
//
LOCUS       DAOSCR010000082         5866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8629_length_5866_cov_3.233006, whole genome shotgun sequence.
ACCESSION   DAOSCR010000082 DAOSCR010000000
VERSION     DAOSCR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8629_length_5866_cov_3.233006"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1548
                     /locus_tag="PLT69_03345"
     CDS             241..1548
                     /locus_tag="PLT69_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13508.1"
                     /translation="MHLHAAHEGVRSSSRSGLGNLPRLLLSLLAFSALFAFTGGCSKG
                     EGGGPVAAEEGTYERGYFVDSPVMGLDYSSDSFAGETESDGGFLYSEGQVITFSIGDI
                     VLGRTRGDRIVTPVTLGGSLADVTNTRVINIARLLQSLDADRNPANGITITPSVKQAL
                     AGIRIDLARPDIDNDAGIAELFERLSGMGIYPEEAGELGLVSAEEARLHLEDTVTRIE
                     LEEEAAQEALRSIPVHAFITNGYTGVIMIPGQKIRLVAYAFGGTPPYVYSWSLDGASP
                     FSTRQDAGSYTAPSPSDPVLSLRVADSLNAAYTAFKRIHVQPSQSATGLSYPEDSIPT
                     ARIISPRDGDTFAMGEVVTFKALITDGDLPIHYGWLSNKPSNVMDTAHESVSNVAPRA
                     YLVSIPVTLKAHGDHSIGIAVQDTPVKGKGPDNHASLVSIRVY"
     gene            2283..>2745
                     /locus_tag="PLT69_03350"
     CDS             2283..>2745
                     /locus_tag="PLT69_03350"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017221940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOJ13509.1"
                     /translation="MKTRITELLGIKHPIVLSGMSWISVPKMVAAVSNAGGLGILATG
                     PLDAQQTRAAIREIKSLTNKPFGTNTTLLFPGAAENAMVALEEKVPVINFSLGKGDWI
                     VKAAHEYGGKVIATVVNARHAKRAQDYGCDALLVTGHEAAAHGGDATTFCLV"
     assembly_gap    2746..2755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2756..3421
                     /locus_tag="PLT69_03355"
     CDS             <2756..3421
                     /locus_tag="PLT69_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019442282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOJ13510.1"
                     /translation="IANAVNIPIIAAGGIADGRGLAAALALGADGAAMGTRLMTTKES
                     PLHENYKQLSIQKEVYDTIYSTRFDGLGCRVLDTKAARKAIRQGLNLRKLIEAIPNSM
                     IISKQLHLPYFKLFLGVLASGWKNARQLAFMANAFKAIRIATEDGDTVHGVLPVGQAT
                     GLMRDIPTVAEVIERTVAQAEAISFELAKKAGLKEAAERTPLAEKPKPSRGRKASDKG
                     KKA"
     gene            complement(4115..5104)
                     /locus_tag="PLT69_03360"
     CDS             complement(4115..5104)
                     /locus_tag="PLT69_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOJ13511.1"
                     /translation="MIKTAITDMFSIEYPIICGAMMWLCTPKLCAAVSNAGGMGNLTA
                     GNYETEEAFRSAIRETKRLTDKPFMVGITILPSVRITSEHHAMYARVCAEERVAGIEV
                     SGTPLDKALGKKVMSDLKDAGVKLFHKVGSVRHAVHAEKAGYDGVYAAGFEEGGHPLD
                     DDVTTMVLTPRIVDSGKIPVVTVGGIADGRSLAAALTLGAQGVMMASRFIATTECEVH
                     DNIKQELIKRQENETTLICKSIGLQGRALKNKIISEVLNAEAQGKGIEALIPLIAGQR
                     IKKAWETGDVDDAPLMVGQSIGHIHKIMSCRELLATMHDEAVMHLRKASSLVQ"
     gene            5277..>5866
                     /locus_tag="PLT69_03365"
     CDS             5277..>5866
                     /locus_tag="PLT69_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOJ13512.1"
                     /translation="MHYSFSSRDYVILALSYVFLGISAFHGRAADVLPLIGAVPAWVF
                     LLAGVLLNLWCIPLPGIHPRISGRAAQQALPAPRAAAPDAAVQERVAAEAEPVPVPPP
                     SVPRAEEKLGRGLAKTHKGFIDRIAAVFRRSAKLDASILAELEETLVGTDIGVRTAYA
                     LFEAVQGKMQSLEGPDAAMEAIRQEVESILAARENPL"
BASE COUNT         1119 a         1874 c         1763 g         1100 t
ORIGIN      
        1 acgtataacc cacgaatatt aaatggtcgc ccaaagcgca tgaatcttgc aatgcattca
       61 tataattgtc gttttttgtt ccgttggtga tggcatgagg atggttcggg ctgaccgcct
      121 atcactacgt ggcggcctcg tccgaggatg aaccgttgag aacacgtcgt cccctggggc
      181 atagggatga cgcatgctcc aggaatactg acgattcgag aagaacaggg gagaaaaacg
      241 atgcacctgc atgcagcaca cgagggagtc cgctcgagtt cgagatccgg ccttgggaac
      301 ttgccccgcc tcctcctgtc tctcttggcc ttttccgccc tttttgcctt caccggcggg
      361 tgcagcaaag gggaaggggg cgggccggtt gccgccgagg aaggcaccta cgagcgtggg
      421 tacttcgtgg acagccctgt catggggctc gactactctt ccgattcctt cgccggcgag
      481 accgagagcg acggcgggtt tctgtacagc gaaggccagg tgatcacctt ttccatcggt
      541 gacatcgtcc tcggcaggac gaggggcgac cgtatcgtca cccccgtcac cctcggggga
      601 agcctcgcgg acgtgacgaa tacacgcgtc ataaatatcg caaggctcct ccagtccctc
      661 gatgcggacc ggaacccggc aaacggcata accataacgc catccgtgaa gcaggcgctc
      721 gctggcatca ggatcgacct tgcgcggcct gacatagaca acgacgcggg catcgcggag
      781 ctcttcgaga ggctcagcgg catgggcatc taccccgagg aagcgggaga gttgggcctg
      841 gtctccgcag aagaggcaag gctccacctc gaggacacgg tcaccaggat cgagctcgag
      901 gaggaagcgg cccaggaggc gctcaggagc atcccggtcc atgcattcat aaccaacggc
      961 tacaccgggg tgatcatgat accgggccag aagatccgac ttgtcgccta tgccttcggc
     1021 gggacacccc cttacgtgta ctcgtggtcc cttgacggcg catcaccctt ctcgacgcgc
     1081 caggacgccg ggagctacac cgccccaagc ccgtccgatc ccgttctctc cctgagggtt
     1141 gccgacagcc tgaacgcagc ctacacggcc ttcaagcgca tccacgtcca gccctcccag
     1201 tcagccacgg gcttgtcgta tcccgaagac tcgatcccga cggcgaggat catctccccc
     1261 agagacggcg acaccttcgc catgggcgag gtcgtcacct tcaaggcgct cataacggac
     1321 ggggacctcc ccatccatta cgggtggctc tcgaacaaac cgtcaaacgt catggacacg
     1381 gcccacgaga gcgtctccaa cgtcgcgccc agggcatacc tcgtctccat acccgtgacg
     1441 ctcaaggcgc acggggatca ttccataggc atagcggtgc aggacacgcc ggtaaagggc
     1501 aagggcccgg ataaccacgc ctccctcgtg tccatcaggg tgtactgatt ttcctgaggg
     1561 ctgtgggacc tcgttgaagt cggacgcccg cccgactggc ctttttggca gtggagctcg
     1621 ttcttcgagg aacccttcct gggtgaagac agctgaccat gccgaaggcg tccgtcctcc
     1681 ccttcgagag tgatgccaac catgccatga ctggggcgcc gatggggcaa ccgtgacctt
     1741 gaccgcgcca cggtgtcaac ccatgcacgg gcaggggcgc cggtctcgaa gatcatccga
     1801 ggatctcgca tagctgtcaa cccgtgccca ggcaggggcg catgcaagga ggatgacgat
     1861 cctgcaaggg cggcccgatg aagacctcct catgtgcaag gcatcggccc cggcgtccac
     1921 cgtcatccct caggcctaca tctgcgggca gtctctttgt cgctcgtgtg cacgagccga
     1981 cccgagaggc ccaaggcttc agccacgaag aggcagccga agcgacgatg ataccctttc
     2041 ccgtgttgcc gatatcggcc tgtggtgtat aatctccaag gtggacgatc gggagacatg
     2101 gggggagtca gccgccctcc atcgtgcccg ccgcatgcat cttgaggtgc gtcgcggccg
     2161 gtttccatcc ttgacaagaa cgcgctcggt gcggtagatg acttatagtt taactttaat
     2221 ctttacatca cagggaagga agccgagacg cgctctgcaa taaaaaacta gaggagcagc
     2281 caatgaagac acgtatcacc gagctcttgg gcatcaagca tcctatcgtt ctctccggca
     2341 tgagctggat cagcgtcccc aagatggtcg ctgccgtatc caatgcaggc ggcctcggga
     2401 tcctcgcgac cgggcctctg gatgcgcagc agacgagggc cgcgatcagg gagatcaagt
     2461 cgctgacgaa caagcctttc gggaccaaca ccaccctcct cttccccggt gcggccgaga
     2521 acgccatggt cgccctcgag gagaaggtcc cggtgatcaa cttctctctc ggcaagggtg
     2581 actggatcgt gaaagccgcc cacgaatacg gcgggaaggt gatcgcgacg gtggtcaacg
     2641 ccaggcacgc caagcgcgcc caggattacg ggtgcgacgc cctgctcgtg accggccacg
     2701 aggcggcggc ccacggcggc gacgccacca ccttctgcct cgtccnnnnn nnnnnatcgc
     2761 caacgcggtc aacatcccca tcatcgcggc aggggggatc gcagacggca ggggcctggc
     2821 agctgcgctc gccctcggag cggacggggc ggccatgggc accaggctca tgaccacgaa
     2881 ggaaagcccc ctgcacgaga actacaagca gctctccatc cagaaggaag tgtacgacac
     2941 catctactcc acgcgcttcg acggcctcgg ctgcagggtg ctcgacacga aggccgccag
     3001 gaaggccata cgccaggggc tcaacctcag gaagctcatc gaggcgatcc ccaactccat
     3061 gatcatctcc aagcagctcc acctgccgta cttcaagctc ttcctgggcg tgctcgcatc
     3121 cggctggaag aacgcacgcc agctcgcctt catggcgaac gcgttcaagg cgatccggat
     3181 tgcgaccgag gacggcgaca ccgtccacgg cgtgctgccg gtcgggcaag ccacgggcct
     3241 catgagggac atacccacgg tcgccgaggt catcgagcgg accgttgccc aggcagaggc
     3301 gatcagtttc gagctggcga aaaaggccgg cctgaaagag gcggctgaaa ggacgcctct
     3361 cgcagagaaa cccaagccca gccggggaag gaaggcctcg gacaagggca agaaggcgta
     3421 agaaagaacg tgtcggcgca ggagggagat cgaggctcgc tgctctcccc ggaagcccga
     3481 ttcttcacgg cacgatcgtt tagcgtagcg agacagcctg aaggacaaga agggcggcac
     3541 cagagacagg tgccgccctt cctctttcct atgcccagcc atagcccggc cttgtgaagc
     3601 gagcatcggg cgatgatgtc aagcaatccg tcgcgctcac cctgcgtcag ggggaggaca
     3661 agcttcggac cccttcatga taacggcaag aatgctttgc acgtttgatt ctggtctctg
     3721 tctttcgatg atcgaaacca ggtcgctcga ccttcacacc gcgtgacgcc tctctcaccc
     3781 gtcggcacct gcctgcttgc ctatgcctcg gcagaccggg atcatgcagg tgtcccgggc
     3841 gagatcgacc ctcgagtctg tatggaagac gtctcggatt gttggccatg ggatgaggtc
     3901 tgcttgcgct tggggatgtg atacaccatc ctcagggaag acgtcctcga ttggtgccct
     3961 tgcgaaggta tcgagggtcg ccttcatgca tggagagcgt gagccgctcc tttccgcgcg
     4021 gctatctcgc ccggaaagga gcgggacgac atgtgagaag attcaccggc tcatgggtgt
     4081 tccacgatgc cttggggaga tgagggccgg gatctcattg cacgagggaa gacgcctttc
     4141 tcaagtgcat gaccgcctcg tcgtgcatgg tggcgaggag ctccctgcag ctcatgatct
     4201 tgtggatgtg gccgatcgac tggcccacca tgagcggggc gtcatccacg tcacccgtct
     4261 cccaggcctt cttgattctc tggcccgcga tcaggggtat caacgcctcg atacccttgc
     4321 cctgggcctc ggcgttgagc acctcggaga tgatcttgtt cttgagcgcc ctgccctgca
     4381 gccctatgga cttgcagatg agggtcgtct cgttctcctg cctctttatg agttcctgct
     4441 tgatgttgtc gtggacctcg cactcggtcg tcgctatgaa cctgctcgcc atcatgacgc
     4501 cctgggcgcc gagcgtgagc gccgccgcaa ggctcctgcc gtccgcgatg ccgccgacgg
     4561 tgacgaccgg gatcttcccc gagtcgacga tcctgggggt gaggaccatg gtcgtcacgt
     4621 cgtcgtcgag cgggtgaccg ccctcctcga accccgccgc atacactccg tcgtagcctg
     4681 ccttctctgc atgaacggcg tgcctgaccg accccacctt gtggaagagc ttcacgccgg
     4741 cgtccttgag gtcggacatg accttcttgc ccagggcctt gtcgagaggg gtccctgaca
     4801 cctctatgcc ggccacccgt tcctcggcgc acaccctcgc gtacatggcg tggtgctccg
     4861 aggtgatccg gaccgagggc aggatcgtga tacccaccat gaagggcttg tccgtgagtc
     4921 tcttcgtctc ccggatggca gagcggaagg cctcctcggt ctcgtagttg cccgccgtga
     4981 ggttccccat cccgccggcg ttcgatacgg ccgcgcacag tttgggcgtg cacagccaca
     5041 tcatggcgcc gcagatgatc gggtactcga tgctgaacat gtccgtgatc gcggtcttta
     5101 tcatgtctcc cccatgttta aagttgaact ttatgcttca tgtttaaccc gtctcttcct
     5161 ccttgtcaac gggaaaaagc cgtcaacccc ctcaccgttt gtgttccccg tccttgtcgc
     5221 gggctcggcg ttgtggtagg aatacccccg catcacgtta ctcctggagg gcaatcgtgc
     5281 actattcttt ctcgagccgt gactacgtca tcctcgcctt gagctacgtg ttcctaggca
     5341 tctcagcgtt tcacggccgg gctgcagacg tcctcccgct catcggcgcg gtcccggcat
     5401 gggtgttcct cctggcgggt gtcctcctga atctctggtg catccccctt ccgggcatcc
     5461 atccgaggat ttcgggcagg gccgcacagc aggctcttcc tgcaccccgg gcggccgcac
     5521 ctgatgcggc ggtgcaggaa agggtcgccg ccgaggccga gccggtaccg gttccccctc
     5581 cctccgtgcc gagagctgaa gagaaactgg gcaggggtct ggccaagacc cacaagggtt
     5641 tcatcgatcg catagcagcc gtcttcaggc ggtcggccaa gctcgatgca tcgatcttgg
     5701 ccgagctcga ggaaaccctg gtcggcacgg acataggggt gcgcaccgcg tacgccctgt
     5761 tcgaggccgt gcagggcaag atgcagtctc tcgagggtcc ggacgccgcc atggaggcca
     5821 tacgccagga ggtggaatcg atccttgccg ccagggagaa cccact
//
LOCUS       DAOSCR010000083         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8662_length_5851_cov_3.425293, whole genome shotgun sequence.
ACCESSION   DAOSCR010000083 DAOSCR010000000
VERSION     DAOSCR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_5851_cov_3.425293"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLT69_03370"
     CDS             complement(<1..231)
                     /locus_tag="PLT69_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13513.1"
                     /translation="MDRAVRAIVLVCAVEAAFRLASPLVPIDVLAWTLVARVVEAACI
                     LATAFGESGIKAASIPFELAVGLGASAAFGCLV"
     gene            486..2360
                     /gene="selB"
                     /locus_tag="PLT69_03375"
     CDS             486..2360
                     /gene="selB"
                     /locus_tag="PLT69_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOJ13514.1"
                     /translation="MKRVVIGTAGHVDHGKTSLIKAITGIDCDRLKEEKERGLTIELG
                     FASMTLPSGERVGVVDVPGHVRFIRHMLSGASGIDLVLLVIAADEGVMPQTREHVQIL
                     ELLGIERGVVALTKKDLVENDLLELAASEVRDFLSATSLRDAPVVAVSSVTLEGIPEL
                     MRTIEEQVGLASERRATGIPVLPVDRVFTVKGFGTVVTGTLARGSLAPGMDVEILPSG
                     TSAKIRNIQVHDTDVQEALAGLRTAVNLQGVSTSEIERGQWVAPSGVFRPTKAVDARV
                     TLVGRPPKSGIRMHIGTAEVQGTMSVHASGEAVYARVRLKRPVVAAFGDRFILRSGNG
                     ALTVAGGVVLNPHPKRRFSLEVMEALCSDDIQRQVEGLAVDAGVRGLSKQEIEAAFAR
                     DPEQAVRGVEALASSGRIVRYDATADLYVHRRYLDRLEEQITAELDRFHAEFPASPGM
                     PREHLRSSVAPGADAKLFHKAILNLAKKGAVAQDGPVLARSGFRPGLESRRTDLGRKI
                     AGIIDEAGFEPPRLQELAQSLDIALKEVQEIVGFLSREGLVVKVKDDIHISRRNEETL
                     RRRVREFLEERGSMTPVDMKDVLGVSRKYAIPYLEYLDRIRFTLRVGDVRRLASAPNT
                     "
     gene            complement(2709..3386)
                     /gene="bamD"
                     /locus_tag="PLT69_03380"
     CDS             complement(2709..3386)
                     /gene="bamD"
                     /locus_tag="PLT69_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOJ13515.1"
                     /translation="MGRTAALIVIVAILACVSGCAHEPEGKRPASELFEEGASLAREG
                     KVEKAAEAFMKVRTYYPSDELARRALLATADLYFDTGDYASALKSYQEYRLLYPTDAQ
                     AGYCLFRIGMCHFNQMSTYDRDQTETNRAVTAFESFLAAYPSSPHVEMARACLGEARA
                     RLGQNMLSVGRFYLRKGNLEAACARFRAVREKYPEAAPRDELDELVARACSSGPARAD
                     NPADKRQ"
     gene            complement(3951..5066)
                     /locus_tag="PLT69_03385"
     CDS             complement(3951..5066)
                     /locus_tag="PLT69_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HOJ13516.1"
                     /translation="MRRLIVVFVATILALEVCAVPGARAETPVVGLVVPLSGRWEAVG
                     QKILRGVMCASGVFGGEGLGIEFVVKDYGDDMGKLASIVDGLASDDRVLAVIGPVGEK
                     AVEVACSHATPAGLPVISFTQAEPAGSKGTVCFRNFLTVETQAHALLAAAASLGVTRF
                     AVFGPDDQFGKTLAHHFTRLAPTYGVRVLAARTYPPTHPDFQLELRELFKGMGEGARQ
                     VQALLVPDEAANAGMIASYLAYLKRQGIRLFGPSLWDSPDLMRAGGANVEGAVFLSGF
                     FPHGYSSAARDFAEAFRPYGANPGVWEASAYDTARIVSNILQEGVRSRKGVAARLASV
                     RRYDGASGLTSLFPDGSLEKQPRVLSVRGGAVVEIFP"
     gene            5685..>5851
                     /gene="tatB"
                     /locus_tag="PLT69_03390"
     CDS             5685..>5851
                     /gene="tatB"
                     /locus_tag="PLT69_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOJ13517.1"
                     /translation="MFGIGGQELLVILVIALIVLGPKNLPEIARTLGRAFGQFQRATD
                     DIKREFESSLT"
BASE COUNT          994 a         2046 c         1942 g          869 t
ORIGIN      
        1 cacgaggcag ccgaaggcgg ccgaggcccc caggccgacg gcgagctcga aggggatgga
       61 ggctgccttg atgccgctct cgccgaatgc cgtggccagg atgcaggccg cctccacgac
      121 cctcgcgacg agcgtccagg caaggacatc gatgggtacg agcggcgagg cgaggcggaa
      181 ggccgcctcc accgcgcaca cgagcacgat cgcccttacg gccctgtcca tgtctcccac
      241 atatagaagg aaacacaccc gtgttgcaag aaacccgggc aagggtccgc cccctcactc
      301 ccctgacgct cacccgcact tgggactcgg cggcgggctc cgccgcacgg gttatccgcc
      361 cctgacgggt cttaagcggc cccgagcacc ctcgaccacc cggacggcac aaaacccctc
      421 tgttccccac cgagcccgcc tgccggaatt gaccgggagc gcgtgatgcg gctatactgc
      481 ctgccatgaa gagggtcgtc atcgggaccg cggggcacgt ggaccacggc aagacctcgc
      541 tcataaaggc catcacgggc atagactgcg acaggctcaa ggaggagaag gagcgcgggc
      601 tcaccatcga gctcgggttc gcctccatga ccctgccctc cggcgagcgc gtcggcgtgg
      661 tggacgtgcc gggccacgtg cgcttcatca ggcacatgct ctcgggcgcc tcgggcatcg
      721 acctcgtgct cctcgtgatc gcggccgacg agggcgtcat gccccagacg cgcgagcacg
      781 tgcagatcct ggagcttttg ggcatagagc ggggggtcgt ggcgctcacc aagaaggacc
      841 tggtggagaa cgacctgctc gagctcgccg catccgaggt ccgcgacttc ctctccgcca
      901 cgtccctgag agacgcacct gtcgtggccg tgagctcggt gacgctcgag gggatccccg
      961 agctcatgag gaccatcgag gagcaggtgg ggctcgcctc ggagcggcgc gcgacgggca
     1021 tacccgtgct ccccgtggac cgcgtcttca ccgtcaaggg gttcggcacc gtcgtcaccg
     1081 gcacgctcgc gaggggatcg ctggccccgg gcatggacgt ggagatcctc ccgtcgggca
     1141 cttccgcgaa gatcaggaac atccaggtgc acgataccga cgtccaggag gctctggcgg
     1201 ggctgcgcac ggccgtgaac ctccagggcg tgtccacctc cgagatcgag cgcggccagt
     1261 gggtcgcgcc ctccggcgtc ttcaggccga caaaggccgt ggacgcaagg gtcaccctgg
     1321 tgggccggcc gcccaagagc ggcatccgca tgcacatcgg caccgcggag gtgcagggca
     1381 ccatgagcgt ccacgcatcc ggagaggccg tctacgcgcg ggtacggctg aagaggccgg
     1441 tcgtggccgc tttcggggac cggttcatcc tgagatcggg aaacggcgcc ctcaccgtgg
     1501 caggcggggt ggtgctgaac ccgcacccga agaggaggtt ctccctcgag gtcatggagg
     1561 ccctctgctc ggacgacatc cagcgacagg tggaaggcct ggccgtggac gcgggggtca
     1621 ggggcctgtc caaacaggag atcgaggccg ccttcgcccg ggaccccgag caggccgtcc
     1681 gcggggtcga ggcccttgcg tcctcgggca ggatcgtgag gtacgacgcg accgccgatc
     1741 tgtacgtcca ccgaaggtac ctcgaccggc tcgaggagca gataacggcc gagctcgacc
     1801 gcttccacgc ggagttcccc gcctcgccgg gcatgccgcg cgagcacctg cgcagctcgg
     1861 tcgcccccgg ggcggacgcg aagctcttcc acaaggcgat cttgaacctc gccaagaagg
     1921 gggcggtcgc gcaggacggg ccggtgctcg cccgctcggg gttcaggccg ggcctcgagt
     1981 cacggcgcac tgacctcggc aggaagatcg ccggtatcat cgacgaggcc ggcttcgagc
     2041 cgccacgcct ccaggagctt gcccagtcgc tggacatagc cctgaaggag gtgcaggaga
     2101 tcgtggggtt cctctcgagg gaaggcctcg tggtgaaggt caaggacgac atccacatct
     2161 cgaggaggaa cgaggagacc ctcaggcgcc gggtgcggga gttcctcgaa gagcgcggat
     2221 ccatgacgcc ggtggacatg aaggatgtcc tgggcgtgtc caggaaatac gcaatcccct
     2281 acctcgagta cctggaccgc atccgcttca ccctcagggt gggggatgtg cgaaggctcg
     2341 catcggcgcc gaacacgtga ggcggcttcg aggggaacgg ccgcaagcca cgcccggcgg
     2401 gcgagccccg cgctttcacc gggggcgtca aacccgggat caggaccctg ccccagggcc
     2461 tcacttcctc ggcatcagct tgcggacgag gaacgccctg tccctgagct tgagcttcca
     2521 gtacagcggg ctcaacacga gcgcgcgaat gatctccctc ttcgtcatgg ggcattcctc
     2581 cgtcaatttc ctggccacat acggaggaat gccatttccg tgccagaaat tacatagaac
     2641 aataatttcg gtacactatg agatttgaca ggggagatgg cggcccggga gacccttgat
     2701 atgtcgattt attgacgctt gtcggcgggg ttgtccgccc gcgcagggcc actcgagcag
     2761 gcccgggcga cgagctcgtc cagctcgtct cggggagcgg cctcggggta tttctccctc
     2821 accgccctga acctcgcgca tgcggcctcg aggttgccct tcctgaggta gaacctcccc
     2881 accgagagca tgttctggcc gagcctcgcc cttgcctccc ccaggcatgc cctggccatc
     2941 tcgacgtggg gggaagacgg gtaggcggcg aggaacgact cgaaggcggt caccgccctg
     3001 ttggtctcgg tctggtcacg gtcgtaggtg gacatctggt tgaagtggca catgccgatg
     3061 cggaagaggc agtacccggc ctgggcgtcc gtggggtaga ggagcctgta ctcctggtac
     3121 gacttgaggg ccgaagcata gtccccggtg tcgaagtaga ggtctgcggt ggccaggagc
     3181 gccctgcgcg cgagctcgtc tgacgggtag tacgtcctca ccttcatgaa ggcctcggcc
     3241 gccttctcca ccttgccctc cctcgcaagg gaagcccctt cctcgaagag ctcggaggcg
     3301 ggcctcttgc cctcgggctc gtgggcgcac cccgacacac aggcaaggat ggcgacgatc
     3361 acgatcagcg ctgccgttcg gcccatacgg ccaccaagac taggacagat cacgtgggcg
     3421 atcaagggat ttttggcctc acgcccggcc ggcctcagtc aaggacgagc ccgggacggg
     3481 cccgcacgca cccccgcacg tggtttccga gtacgacgcc tttttctgga tacgcgctcg
     3541 atcgacgcgc gcatggccgc cccgtgggac gtgcgaggtg tcgtcccagc tgcctcgccc
     3601 ccaaggcgcg gcatgcaggg cagcccttca caggatttgc ctaaaaacgg cctcaggccc
     3661 tgtcgcatgg cgcccacgat cacaccgcca cgggcgaggg cgacggggct gctcctcttt
     3721 cagaaaccat gcgccgccgc atcacgaggg cccacccgct ttcggcccgc gcaagcccga
     3781 tgcacagccc gccgccaagc aacgggcgaa ggcggcgacg cctgcaccgt ggcggcgccg
     3841 gcctcccgcg ccccccacgc agacatgggc gccgagagaa ggccccctcc acgacccaaa
     3901 aaggcgtcct ttggcccggc tcccccaggg gcaggcttac ggatgcgaga tcacgggaag
     3961 atctccacca cggcgccgcc ccgcaccgag agcaccctcg gctgtttctc gagcgacccg
     4021 tcggggaaca ggctcgtgag gccgctcgcg ccgtcgtacc gcctgacgga agcgagccgc
     4081 gcggcgacgc ccttgcggga acgcacccct tcctgcagga tgttcgagac gatgcgcgcc
     4141 gtgtcgtagg cgctcgcctc ccacaccccg gggttcgcgc cgtacggcct gaacgcctcc
     4201 gcgaaatccc tcgcggcgga cgagtagccg tgcgggaaga acccgctcaa gaagaccgcg
     4261 ccctcgacgt tcgccccacc tgcccgcatg aggtcggggc tgtcccacag gctggggccg
     4321 aagagcctta tgccctgcct cttgaggtag gcgaggtacg aggcgatcat gcccgcgttc
     4381 gcggcctcgt ccggcacgag caacgcctgc acctggcgtg ccccttcgcc catgcccttg
     4441 aacagctccc tgagctcgag ctggaagtcg gggtgcgtgg gagggtaggt ccttgcggca
     4501 aggacgcgca cgccgtaggt gggcgcgagt cgggtaaagt ggtgcgcgag ggtcttgccg
     4561 aactggtcgt ccgggccgaa gaccgcgaac ctcgtgaccc cgagcgaggc ggcggcggcc
     4621 agcagcgcat gcgcctgcgt ctcgaccgtg aggaagttcc tgaagcacac cgtgcctttc
     4681 gaccccgccg gctccgcctg cgtgaacgag atcacaggca ggccggccgg cgtggcatgc
     4741 gagcaggcga cctcgaccgc cttctcgccc accggcccga tgacggccag gaccctgtcg
     4801 tcagaggcga gcccgtccac gatggatgcg agcttgccca tgtcgtcccc gtagtccttc
     4861 accacgaact cgatgcccag cccttcgccc ccgaagaccc cggaggcgca catgaccccg
     4921 cggagaatct tctggccgac cgcctcccac ctcccgctca gcgggacgac gagccccacc
     4981 accggcgtct ccgcgcgcgc gccggggacg gcgcacacct ccagcgccag gatggtcgct
     5041 acgaagacta cgatcaggcg cctcatgctc tcggtatccc gctgggcata gtaacccccg
     5101 gaacatccgg ggtcaaggcc ggcagccttg ccccggggaa agaccgctcc ggggccagac
     5161 cgcaggtgtg cacgggccag actgaggacg tagatcgact ggagacaggc cacccaccca
     5221 accccgatgt gcgggggcgc gacgcctgtt cgtgggcctc gttccggagg gaccttcctc
     5281 ccaattccgg tgagtacgtg cgtgacggcg tccagcctgc ctatggaatc aaatctccca
     5341 ccagaccccc ggtgtgcgcg ggcgagaggg tccacatcct gctcgggata cacggccctg
     5401 aacagacctc cggtgcacac gggcgcggcc ctcttcgcgg cccacggcga aggcgccgga
     5461 tgtcccgagg ggtcagcctg cacgcaccga gtcctcggca ggcacgcgcg agaccccgcg
     5521 ctcggcgccc ttcaaggacc ccgcccccca tgccctgccg tcttccccgg accagggccc
     5581 gcttccgccg cacggttgtg gtcccggcga tcgccgggcg ccgggccgcc ctgcctgcga
     5641 gcacccgttt gactttggcc tgcccatggg ttagacggtg ggacatgttc ggcataggcg
     5701 gccaggaact cctcgtgata ctcgtgatag cgctcatcgt gcttggcccc aagaacctcc
     5761 cggagatcgc acgcaccctg ggcagggcgt tcggccagtt ccagagggcc acggacgaca
     5821 tcaagcggga gttcgagtcg agcctcaccg a
//
LOCUS       DAOSCR010000084         5849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8672_length_5849_cov_2.934588, whole genome shotgun sequence.
ACCESSION   DAOSCR010000084 DAOSCR010000000
VERSION     DAOSCR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8672_length_5849_cov_2.934588"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLT69_03395"
     CDS             <1..962
                     /locus_tag="PLT69_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13518.1"
                     /translation="RLFEKSMALLVAQDARHIRIIAAKDGVIYYLITVNAAESFDPVA
                     ETVSGIKEITSLLAGTHREKVAKIYLLGAGEVSPSDLERHGVEVAGVPAAFAPDTGIE
                     YACLFGTAMGPRYDFTTDRLRTTRTLVRFSRISMVCSLLMIVMSAVWLGLASINHSSA
                     RFLEARAVQESRKVSTLVGRLEKSASTVNLNVDVSRINELMKTYRDFQSEPRFDSLVE
                     AVCELMPEHVYLTRVALERGTQGQGGQSPRVIPEAVEGQRTALSKSFGLTVEGLVNVA
                     YPDSKTVFSRIVSRMNSTYQVKNASFRHTDDTSWFKLECEVMP"
     gene            959..1354
                     /locus_tag="PLT69_03400"
     CDS             959..1354
                     /locus_tag="PLT69_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13519.1"
                     /translation="MRYFKAAVALGLLGALLLTVSVTLHRQSVRNLSREKARLAYLTT
                     MAGQTAVYEEKLESVKKTMPKLELLAAGGSAQIDYEISMTDNDLSRLMEKTRDTYADG
                     IFFLESAVAESTSFGISVTMKGFKLGGNP"
     gene            1351..1815
                     /locus_tag="PLT69_03405"
     CDS             1351..1815
                     /locus_tag="PLT69_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13520.1"
                     /translation="MTMKIDNRLLVLFAPHAAALAAVAAVLFLMPAEAPYRVERPEIL
                     TFIDQLHSVTLTERQPEGRSGVKDVFRHEWTVWGHVPGEGMQARGNPTEKESVEVSLV
                     VDEGPDGYCIINGKRMETGHKADGFTVESIQKDRVTIVRTNGTRETYHVKPY"
     gene            1799..3427
                     /gene="mshL"
                     /locus_tag="PLT69_03410"
     CDS             1799..3427
                     /gene="mshL"
                     /locus_tag="PLT69_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HOJ13521.1"
                     /translation="MSSHTRGITALTVCLTSLALLCSCAVDRAMKRESPVIEKEMASK
                     AQVEPRPQAPMRVEPKEAPHDPLKGKTITLTAKNAPFSEIYAAIAQKAGLDLVIDSRL
                     ISAQPELSTTAAQVPSGVQGQSMQPAGARRSKIEVPTVSITFTKTPLDEALSSVDHAL
                     HLFSSVKRNTLHVKGTESRTFSLTFLSSQKETTIRVGGDVIGSSYLGSSSGSSSGSSG
                     SSSGDTLKGEFSIKDTVTPSSKDIFAQVEETVKSCLSPYGTYSLNRALGFLEINDMRD
                     TIERIDAYIKTLKAHYNSQVLITAKVIEVSLKDTSRYGIDWSSIHGTIRDYAFNPISQ
                     HLSLDTSTLTPALEIQVGSSKHGFDAALNALAEFGDIKVLSNPRIRVTNGQSALISVG
                     TSSSYIQEIKVTTTSVEGGSTVITPEVTVGSIFDGVMLGVVPSIDLDNYAVNLSITPI
                     KSRIVTLEERTVHDNLYTLPKVDLEEVSSQIRVKSGNIIALGGLISKDLATENTKVPI
                     LGDIPYLGYLFKQESKSVRTAELVILLEPVILSE"
     gene            complement(3550..4290)
                     /locus_tag="PLT69_03415"
     CDS             complement(3550..4290)
                     /locus_tag="PLT69_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ13522.1"
                     /translation="MYDLHIHTRYSSDGQHDPAALVEMAGAAGLRGIAFTDHMDVTAA
                     RESRLIASTRGVRLFTGVEISTVFEGEERHLLLYGFEPSDRVLGAFLEETCRRAWERA
                     HEVIEVFTGMGFSVGAEDVDGWGSSMPTGVSLLDALLRRNRHDPRLAPYLEGPKASSP
                     YMSFYQDYALGDVGEIVRRALPGLVDTMRLLAGRGILVLAHPCDIPVESLERLVPEGL
                     RGIEAYSTHHTGRQELRLVEAARTLGLW"
     gene            4358..5008
                     /locus_tag="PLT69_03420"
     CDS             4358..5008
                     /locus_tag="PLT69_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOJ13523.1"
                     /translation="MKGEQYLIDQIKPQESWRVFRIMAEFVDGIEQLTPLEPAVTIFG
                     SARTKPGEKFYVMAETLGRLLAEEGFTVITGGGPGIMEAANKGAFEAGGQSVGLNIVL
                     PFEQKLNPYTTIHINFRYFFVRKVMFVKYAMSYVIFPGGFGTMDELFEALTLIQTDKL
                     KPFPVILVGSDYWAGLLEWINETMLGANMISPEDRTIFTLVDSPKDALEIIKNSKV"
     gene            complement(5033..>5849)
                     /gene="proC"
                     /locus_tag="PLT69_03425"
     CDS             complement(5033..>5849)
                     /gene="proC"
                     /locus_tag="PLT69_03425"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ13524.1"
                     /translation="GVEMLSIGFVGAGNMAEAIVAGLIGRSSVETSTVACTDIVEARM
                     DHMARVYGVRKVKDAHELASLCEVVVLAVKPGQVRDAVAGMKGAHADRLVVSIAAGVP
                     LKALEAMLKPGSRIVRVMPNACCLVGQGASVMCATGACSEEDRAAVRRIFESVGMCIE
                     LDERHLDAVTALSGSGPAFCFVFLEALADGAVRSGLPRDLAYMLAGATLKGAAEMALR
                     LQKHPGQLKDMVTSPGGTTIEGIAALEAGAFRSSVMEAVRAAWLKAREISEGW"
BASE COUNT         1150 a         1956 c         1790 g          953 t
ORIGIN      
        1 tcaggctctt cgagaagtcc atggccctgc tcgtggccca agacgcacgg catatccgca
       61 tcatcgcagc caaagacggg gtcatctatt acctgatcac cgtgaatgcc gcagaatcct
      121 tcgaccccgt cgcggagacc gtatcgggca tcaaggagat cacctccctg ctcgccggca
      181 cccacaggga gaaagtcgcc aagatctatc tcctcggcgc cggggaggtt tccccatccg
      241 atctggagag gcacggcgtg gaggtggcag gggtccccgc ggcattcgct cccgacacgg
      301 gcatcgagta tgcatgcctg ttcgggaccg cgatgggtcc ccggtacgac ttcaccaccg
      361 acaggcttcg aaccaccagg acgctcgtga ggttctcacg catctccatg gtgtgttccc
      421 ttctcatgat cgtcatgagc gccgtgtggc ttggtctcgc atccatcaac cattcgagcg
      481 cacgttttct cgaggccagg gccgtgcagg aatcgcgcaa ggtctccacc ctcgtgggac
      541 ggctcgagaa gagtgcatcc acggtgaacc tgaacgtgga cgtctccagg atcaacgagc
      601 tcatgaagac ctaccgggac ttccagagcg agccgaggtt cgattccctc gtcgaggcgg
      661 tctgtgagct catgcccgaa cacgtctatc tcacgagggt cgcgctcgag agaggtaccc
      721 agggccaggg gggccaatcc ccccgagtga tccccgaggc tgtcgaaggc cagaggacgg
      781 cgctgtccaa gtcgttcggc ctcaccgtgg aagggctcgt gaacgtcgca taccccgatt
      841 cgaagaccgt cttctcgagg atcgtgtcca ggatgaactc cacgtaccag gtcaagaacg
      901 cctccttcag gcacacagac gatacgagct ggttcaagct cgagtgcgag gtgatgccgt
      961 gaggtacttc aaggctgccg tggcgctcgg cctcctgggc gcgctcttgc ttaccgtatc
     1021 ggtcacgctg caccgccaga gcgtccggaa cctctcgcgg gagaaggcga gactggccta
     1081 cctcactacc atggccggcc agacggccgt gtacgaggaa aagctcgaat cggtcaagaa
     1141 gaccatgccg aagctcgagc tcctcgcggc cggcgggtcc gcccagatcg actacgagat
     1201 cagcatgacc gacaacgacc tctcgcgcct catggaaaag actagggaca cgtacgcaga
     1261 cgggatcttc ttcctggaaa gcgccgttgc ggagagcacc tccttcggaa tctcggtgac
     1321 catgaagggc ttcaagctcg gggggaaccc gtgacgatga agatagacaa caggctcctc
     1381 gtgctcttcg ccccgcatgc cgcggcgctg gccgccgtcg ccgcggtcct cttcctgatg
     1441 ccggccgagg cgccctaccg ggtcgagaga ccggagatcc tgacgttcat cgatcaactc
     1501 cactccgtga ccttgaccga gaggcaacct gagggccgct cgggagtcaa ggacgtgttc
     1561 aggcacgagt ggaccgtgtg gggtcacgtc cctggggaag gcatgcaggc gcgcggcaat
     1621 cccacggaga aggaaagcgt cgaggtaagc ctcgtcgtgg acgagggacc tgacggttat
     1681 tgcatcatca acggaaagag gatggagaca ggtcacaaag cggacggttt caccgtcgaa
     1741 tccatccaga aagacagggt gaccatcgtc cgcacaaacg ggaccaggga gacataccat
     1801 gtcaagccat actaggggga tcacagcgtt gaccgtctgt cttacgtccc ttgcgctcct
     1861 gtgctcgtgc gcagtggaca gggccatgaa gcgcgagtcg cccgtcatcg agaaggagat
     1921 ggcgagtaag gcgcaggtcg aaccgaggcc gcaggcaccc atgagggtgg agcccaagga
     1981 agcgccccac gacccgctca aaggcaagac cataacgctc accgccaaga acgctccttt
     2041 ctcggaaatc tacgcggcca tcgcccagaa ggcggggctc gacctcgtga tcgattcacg
     2101 gctcataagc gcccaacccg agctctccac cacggccgcg caggtcccat ccggcgtgca
     2161 aggccagtcg atgcagcctg cgggggcgcg gcggtccaag atcgaggtgc cgacggtgtc
     2221 catcacgttc acgaagacgc ccctggacga ggcgctctca agcgtcgacc atgcgctcca
     2281 cctcttctcc agcgtgaaga ggaacaccct gcacgtgaag ggcacggagt cgaggacgtt
     2341 ttcgctgacc ttcctctcct cgcagaagga gaccacgatc agggtgggcg gcgacgtgat
     2401 agggagcagt tacctcggat cgtcgtcggg atcgtcatcg gggtcgtcag ggtcgtcctc
     2461 gggcgatacg ctcaagggag agttctcgat caaggacacc gtcacgccat cctcgaagga
     2521 catcttcgcc caggtcgagg agacggtgaa gtcatgcctc agcccgtacg ggacctattc
     2581 cctcaacagg gcgctgggat tcctcgagat caacgacatg agggacacca tcgagcggat
     2641 cgacgcctac atcaagaccc tcaaggcgca ctacaactcc caggtgctca tcacggccaa
     2701 ggtcatcgag gtctcgctca aggacacgag caggtacggc atcgactgga gcagcataca
     2761 cggaaccatc agggactacg ccttcaaccc catcagccag cacctctccc tcgacaccag
     2821 cacgctcacg ccggccctcg agatccaggt ggggtcgtcc aagcacggct ttgacgccgc
     2881 cttgaacgcc cttgccgagt tcggggacat caaggtgctg tcgaaccccc gcatcagggt
     2941 gacgaacggc cagagcgccc tcatcagcgt cgggacgagt tcctcgtaca tccaggagat
     3001 caaggtcacg accacgagcg tggaaggcgg gtcgacggtc atcacgcccg aggtcaccgt
     3061 ggggtccatc ttcgacggcg tcatgctcgg cgtcgtgccg agcatcgacc tcgacaacta
     3121 cgcggtcaac ctgagcatca ccccgatcaa gagccggatc gtgacgctcg aggaacgcac
     3181 cgtgcacgac aacctctaca cgctgcccaa ggtggacctc gaggaggtct cgtcgcagat
     3241 cagggtcaag agcgggaaca tcatcgcgct cgggggcctc atcagcaagg accttgcgac
     3301 cgagaacacg aaggtcccca tcctcggcga catcccgtac ctcggctacc tgttcaagca
     3361 ggagagcaag tccgtgcgga ccgccgagct cgtgatcctc ctggagcccg tgatcctctc
     3421 cgagtgaggc ccctcacgac ccggacaggg ccatgagcgc ctccacgagc cctgcgtcag
     3481 gctgaccggt gcatccgccg aggcggatcc cgggctttat gcgctcaccg tggaaatcgg
     3541 agcccgcgct caccacaggc cgagggtgcg cgccgcctcc acgaggcgga gttcctgtct
     3601 tcccgtgtgg tgcgtgctgt acgcctcgat ccccctgagc ccctcgggca cgaggcgctc
     3661 aagcgactcg accggtatgt cgcaggggtg tgcgagcacg aggatccccc tccccgcgag
     3721 gagcctcatg gtgtccacga gcccgggcag ggcccgcctc acgatctcgc ccacgtcgcc
     3781 cagggcgtag tcctggtaga agctcatgta cggcgaggac gccttggggc cctcgaggta
     3841 gggtgcaagc ctcgggtcgt gcctgttcct cctcaacagg gcgtcgagca gggacacccc
     3901 ggtcggcatg gacgagcccc acccgtcaac gtcctcggcc ccgaccgaga agcccatccc
     3961 cgtgaagacc tcgatgacct cgtgagccct ttcccaggca cgcctgcacg tctcctcgag
     4021 gaaggcgccg agcaccctgt ccgacggctc gaacccgtag aggaggagat ggcgctcctc
     4081 gccctcgaag accgtggaga tctccacccc cgtgaacagg cgcacgccgc gggtcgatgc
     4141 gatcagccgg gactcgcgcg ccgcggtcac atccatgtgg tcggtgaagg ctatccctct
     4201 cagccccgcc gcgcccgcca tctcgacgag ggcggcaggg tcgtgttgcc cgtccgacga
     4261 atatcgggta tggatgtgca ggtcgtacac ggcggctcaa tgctagcatc accgttgccg
     4321 atagggcaaa cagacgctat cgttttggag ggcggacgtg aaaggcgagc agtacctcat
     4381 agaccagatc aagccccagg aatcctggag ggtgttcagg atcatggccg agttcgtgga
     4441 cggcatcgaa cagctcaccc ccctggaacc ggcggtcacg atcttcgggt cggccaggac
     4501 gaaaccgggg gagaagttct acgtgatggc cgagaccctg ggccgcctgc tcgccgagga
     4561 ggggttcacg gtgatcaccg gcggggggcc tgggatcatg gaggcggcga acaagggggc
     4621 gttcgaggca gggggccagt ccgtcgggct caacatagtg ctccccttcg agcagaagct
     4681 caacccctac accacgatcc acatcaactt ccgctatttc ttcgtgcgca aggtcatgtt
     4741 cgtgaagtac gccatgagct atgtcatctt cccgggcggc ttcgggacca tggacgagct
     4801 cttcgaggcc ctcaccctca tccagacgga caagctcaaa ccctttcccg tgatcctcgt
     4861 cggctccgac tactgggcgg ggctcctgga atggataaac gagaccatgc tgggtgcgaa
     4921 catgatctcg cccgaggaca ggaccatctt caccctcgtc gactcgccca aggacgccct
     4981 ggagatcatc aagaactcga aggtttgaca cccccggggg caaccgggga ggtcaccacc
     5041 cctcggagat ctcccgcgcc ttcagccagg cggccctcac cgcctccatg accgacgacc
     5101 tgaaggcccc cgcctcaagc gcggcgatcc cctcgatggt ggttccgccg ggcgaggtca
     5161 ccatgtcctt gagctggccg gggtgcttct gaagcctgag ggccatctcc gccgccccct
     5221 tgagcgtggc cccggcgagc atgtacgcca ggtccctggg cagcccagag cgcacggcgc
     5281 cgtctgcgag cgcctccagg aagacgaaac agaaggcggg accggagccc gacagcgcgg
     5341 tgaccgcgtc gaggtggcgc tcgtcgagct cgatgcacat gcccaccgac tcgaagatgc
     5401 gcctgaccgc cgcgcgatcc tcctcggaac acgcgcccgt ggcgcacatc accgatgcgc
     5461 cctgccccac gaggcagcat gcgttgggca tcaccctgac gatgcgggat ccaggcttga
     5521 gcattgcctc gagcgccttc agggggaccc cggcggcgat gctcaccacg agcctgtcgg
     5581 catgcgcacc cttcatcccg gcgacggcgt ctctcacctg tccgggcttg accgcgagga
     5641 ccaccacctc gcagagggag gcgagctcgt gtgcgtcctt caccttcctc acgccgtaga
     5701 ccctcgccat gtggtccatg cgcgcctcca cgatgtccgt gcaggccacc gtcgaggtct
     5761 ccaccgagga tcttccgatc aacccggcca cgatcgcctc ggccatgttc ccggcgccga
     5821 caaatccgat ggagagcatc tccaccccc
//
LOCUS       DAOSCR010000085         5841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8688_length_5841_cov_3.668683, whole genome shotgun sequence.
ACCESSION   DAOSCR010000085 DAOSCR010000000
VERSION     DAOSCR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8688_length_5841_cov_3.668683"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>541)
                     /locus_tag="PLT69_03430"
     CDS             complement(<1..>541)
                     /locus_tag="PLT69_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOJ13525.1"
                     /translation="FDEPLGLGHSQEAVQLQIDATRILIYEAKVADVCDPWAGSYFME
                     SLTDKIEEEALAELEKVEKMGGAIAAIENGYMQRAVAKSAYERQKRIEGLEEFVVGVN
                     CFTGENEIEIMVNRVVEATYDPELMETAEERQKASLAALRRERDGRAVLSSLKALEAK
                     AKDEKENLMPVICECVEND"
     assembly_gap    542..551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<552..1675)
                     /locus_tag="PLT69_03435"
     CDS             complement(<552..1675)
                     /locus_tag="PLT69_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOJ13526.1"
                     /translation="MKEDDALRGCGMDRDLVAETRRQWEEEVLEPRVRRFNLPESPTR
                     FYTPLDTDHGDFLEKVGFPGWFPFTAGNSPFDYWRAHARTAALFGYRPDWGGGGKVGK
                     YGGFGTPADYRDYLLRMQAMGRKGGPNMAFDLATQCGYDSDSPHAEGEVGRVGVAVDS
                     FRDFAVIYEAFTGELDIDKVPSNFTINAPACVIIAMYTALAARRGIDPAKLTGTPQND
                     ILKEFISRGTYIFPPRPSMRLFRDTLTFMRKHMPLMNVNSIGGYHIREAGATREQDLA
                     FSMANGIAYIQAGIDGGLMVDDFAPMFTFNAFGGSMEIYHEIAFQRAARRMWAHIVRD
                     RFGARDKRSMMIRQPITAHIGCSSTTLKRPLNNLTRSVIGG"
     gene            complement(1672..2097)
                     /locus_tag="PLT69_03440"
     CDS             complement(1672..2097)
                     /locus_tag="PLT69_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOJ13527.1"
                     /translation="MHAEPRIRVLIAKPGLDGHERGAQVVSYGLRDQGFEVIYTGIRQ
                     RPEQIVDAAIQEDVDVVGLSILSGAHVALTAKVLEGLRSRGAGDILVLVGGVIPDDDR
                     QKLLDMGVAAVYTSGAKIEDIAGFIRSSVRALEGGKGSR"
     gene            complement(2396..3598)
                     /locus_tag="PLT69_03445"
     CDS             complement(2396..3598)
                     /locus_tag="PLT69_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ13528.1"
                     /translation="MDREFEIYFRDMFESIERGNSAERFFQARWTTVKGKVIWVEGRM
                     NLIQWKGEATILLTARDITEIKLREITMQEEAAHLRRENVTLRSSMKDRWRFGDIIGK
                     SQSMQEVYELILNSASSNANIIIYGESGTGKELVAKAIHQLSSRSQKSFVAVNCAAIP
                     ENLMESEFFGYRKGAFTGAISDKQGYLDQANGGTLFLDEVGELGLGMQAKLLRAIEGG
                     GYSPVGSTVTRHSDFRIISATNKNLSELTKRGLMREDFFYRIHIIPITLPPLRQRKED
                     IPLLVEHFLRLYSPGRRLPEVSGQVLESLKNYEWPGNVRELQNVIQRYLTVKRIDFVG
                     PCGVQRRSAITDGHAGIDHEDADNGLQGYTRSVEKKVIMDMLEKCRWNKTKTAQSWGS
                     RARPSSGN"
     gene            complement(3487..4218)
                     /locus_tag="PLT69_03450"
     CDS             complement(3487..4218)
                     /locus_tag="PLT69_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ13529.1"
                     /translation="MAAYENTGGSQERVLEFTERIARLEEEIRGLTRRSTFFELLIKS
                     LPGLFYVFDQNYELHMWNKNVETVTGYLAEEIPVHSIFDIFEGEDLEHVQKAIQHAFI
                     TGSGVAEAVLVTKDGRKIPYFFTGVSTRIEDVQYLIGMGLDISTLKEAQESLKESEAL
                     YRIFAERMTEGVALIHENRILFVNDALSLILSYDSRGPPWEGTSWITWTGSSRSISGT
                     CSSPSSGGTRPSGSSRRGGRPSRAR"
     gene            complement(4869..5774)
                     /locus_tag="PLT69_03455"
     CDS             complement(4869..5774)
                     /locus_tag="PLT69_03455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ13530.1"
                     /translation="MVRLLRLVGAQLRGILSMLLFVADTFMLTVPFYTVALLKWVVPV
                     EAWRRRCTWLLHRVADVWLYFLDLPQAMSRKTKWLVRGLDSVHVPGSCMLVVNHQTWV
                     DIMVLIRLFYRKIPDFKFFVKRELLWLPIVGQAFWAVDFPVMRRYTPEEIRRRPKLKG
                     KDLEIAKKSCEKFKGMPVSIFNFVEGTRFRQDKHARQNSPFTHLLRPKAGGVALVLGA
                     MGEMIHSILDVTIVYPKGVRTFWDYLCGKVDEIRVHVRQIPVTRDLIGDYTDNASYRK
                     YIQEWVNMLWQQKDRRIDEMLLWQG"
BASE COUNT         1020 a         1839 c         1742 g         1230 t
ORIGIN      
        1 cagtcgttct cgacgcattc gcagataacg ggcatgaggt tttccttctc gtccttcgcc
       61 ttcgcctcga gggccttgag cgacgagagc accgcccttc cgtccctctc gcggcgtagc
      121 gccgccaggg aagccttctg gcgctcttcg gccgtctcca tgagttcggg atcgtaggtg
      181 gcttcgacca cgcggttgac catgatctcg atctcgttct ccccggtgaa gcagttcacc
      241 cccaccacga actcctcgag gccttctatc cgcttctggc gctcatacgc gctcttcgcc
      301 acagctcgct gcatgtagcc gttctcgatg gcggctatgg ccccgcccat cttctcgacc
      361 ttctcgagtt ccgcgagggc ctcctcctcg atcttgtccg tgagggattc catgaagtag
      421 gagccggccc acgggtcgca cacgtcggcg accttggcct cgtagatcag gattctcgtg
      481 gcgtcgatct ggagctgcac ggcctcctgg gaatgcccga gcccgagagg ttcgtcgaac
      541 gnnnnnnnnn nccgccgatc accgagcggg tcaggttgtt gagcggtctc ttgagcgtgg
      601 tcgacgagca tccgatgtgg gcggtgatgg gctgcctgat catcatgctc ctcttgtccc
      661 tcgcgccgaa gcggtcccgc acgatgtgcg cccacatcct cctggccgcg cgctggaagg
      721 cgatctcgtg gtagatctcc atgctcccac cgaaggcgtt gaaggtaaac atgggggcga
      781 aatcgtcgac catgagcccc ccgtcgatcc ccgcctggat gtaggcgata ccgttcgcca
      841 tggaaaaggc gaggtcctgt tcgcgggtgg cgcccgcctc gcggatgtga tacccgccaa
      901 tggagttcac gttcatcagc ggcatgtgtt tcctcatgaa cgtgagggtg tcccggaaca
      961 ggcgcatcga gggcctgggg gggaagatgt aggtaccccg cgagatgaac tctttgagga
     1021 tgtcgttctg gggcgtcccc gtgagcttgg ccgggtcgat accgcgcctt gcggcaaggg
     1081 cggtgtacat ggcgatgatg acgcatgcag gagcgttgat ggtgaagttc gacggcacct
     1141 tgtcgatgtc gagctcgccc gtgaaggcct cgtagatgac cgcgaagtcc ctgaacgagt
     1201 cgacggcgac gcccacgcgg ccgacctcgc cctcggcgtg ggggctgtct gagtcgtagc
     1261 cgcactgggt ggcgaggtcg aaggccatgt tgggtccgcc tttgcgaccc atggcctgca
     1321 tgcggaggag atagtcacgg tagtccgcgg gcgtgccgaa cccgccgtac ttgcccacct
     1381 tcccgccgcc gccccagtct gggcggtagc cgaacagggc tgcggtccgg gcatgggccc
     1441 tccagtaatc gaacgggctg tttcccgcgg tgaaggggaa ccatccgggg aacccgacct
     1501 tctcgaggaa atcgccatgg tcggtgtcca gcggggtgta gaatctcgtg gggctctcgg
     1561 ggaggttgaa acgcctcacc cttggttcga ggacttcctc ctcccactgt ctccgggtct
     1621 cggcaacgag gtccctgtcc atcccgcatc ccctcaaggc atcgtcctct ttcatcgcga
     1681 ccccttcccg ccttcgaggg ccctcacgct ggaccttatg aaccctgcta tgtcctcgat
     1741 cttcgccccc gaggtgtaga ccgcggccac acccatatcc aggagcttct ggcgatcatc
     1801 atccgggatc acaccgccca cgaggacgag gatgtccccg gcgcctcttg acctgagccc
     1861 ttccaagacc ttggccgtca gggcgacgtg ggcgcccgag aggatggaca ggccgaccac
     1921 atccacatcc tcctggatgg ccgcatccac gatctgctcg ggcctctggc ggatccccgt
     1981 gtagatgacc tcgaatccct ggtcgcgcaa tccgtacgag accacctggg cgccacgctc
     2041 atggccgtcc agcccgggct tggctatgag aacgcgaatc ctcggctcgg catgcatcaa
     2101 tccatccccc gtcactcgca agcgcctcat ggtcgtgcaa cccgacaacg acccggttcg
     2161 caacgagaga gcaaggatgg tgccagaaag ataattttgg ttactctaat gatattacag
     2221 gtgtttccaa ttctccgccc ggcctggaag accggtcatg gacacgagtg tccctcgcgg
     2281 ataggtggaa aagaccctcc cgaaaggctc aagtgctcga tatcaagatg gaaacaggaa
     2341 ggggcaggcg gtgggacaga cctgtccctc tcaggtcagc taagccccag cctcttcagt
     2401 ttccgctgga gggtcttgcg cgagatcccc aggactgcgc ggtcttcgtc ttgttccagc
     2461 ggcacttctc gagcatgtcc atgatgacct tcttctccac gctcctcgtg tacccctgga
     2521 gaccgttgtc tgcatcctca tggtcgatgc ccgcgtgacc gtcggtgatt gcggaacgcc
     2581 tctggacgcc gcagggaccg acaaagtcga tgcgcttgac ggtcaggtac ctctggatca
     2641 cgttctgcag ctcgcgcacg ttgccgggcc actcgtagtt cttgagcgac tcgaggacct
     2701 gccccgacac ctcagggagc ctcctgcccg gggaatacag cctcagaaag tgttcgacca
     2761 ggaggggtat gtcttccttc ctctgtctca ggggcggcag ggtgatgggg atgatgtgga
     2821 tccggtagaa gaagtcttct ctcatgagcc ccctcttggt gagctccgag aggttcttgt
     2881 tggtggcgga gatgatacgg aagtcggagt gcctggtcac ggtactgccc accggggaat
     2941 accctccccc ttctatcgcg cgcaacagct tggcctgcat cccgaggccg agctcgccca
     3001 cctcgtcgag gaagagcgtg cccccgttgg cctggtcgag gtagccctgc ttgtcgctta
     3061 tcgctcccgt aaaggcccct ttccggtacc cgaagaactc gctctccatg agattctcgg
     3121 gtatggccgc gcagttcacc gcgacgaacg acttctgcga gcgcgagctg agctggtgga
     3181 tggccttcgc gacgagttcc ttgcccgtgc ccgactcgcc gtagatgatg atgttcgcgt
     3241 tcgaggatgc cgagttgagg atgagctcgt agacctcctg catcgactgg ctcttgccga
     3301 tgatgtcccc gaagcgccag cggtctttca tggatgacct gagagtcacg ttctcgcgcc
     3361 tcaagtgcgc agcctcctcc tgcattgtga tctccctgag cttgatctcg gtgatgtcac
     3421 gcgcggtgag caggatggtg gcctcgccct tccactggat gaggttcatt ctcccctcga
     3481 cccagatcac cttgcccttg acggtcgtcc acctcgcctg gaagaaccgc tcggccgagt
     3541 tcccccgctc gatggactcg aacatgtccc tgaaatagat ctcgaactcc ctgtccacgt
     3601 gatccatgat gtcccttccc agggcggacc tctggagtcg tagctcagta tcagggacag
     3661 cgcgtcgttg acgaagagga tacggttttc gtggatgagc gccaccccct ccgtcatccg
     3721 ttcggcgaag atcctgtaga gcgcctcgct ctccttgagc gattcctggg cctccttcag
     3781 ggtgctgatg tcgagaccca tgcctatcag gtactgcaca tcctcgatgc gggtactgac
     3841 accggtgaaa aaatagggga tctttctccc gtccttggtg acgagaacgg cctcggcaac
     3901 gccggacccg gtgatgaatg catgctgtat ggccttctga acgtgctcga gatcctctcc
     3961 ttcgaagatg tcgaagatgc tgtgaaccgg gatttcttcg gcaaggtacc cggtgaccgt
     4021 ctcgacgttc ttgttccaca tgtggagctc gtagttctgg tcgaacacgt agaacagccc
     4081 cggcaggctc tttatgagca gctcgaagaa cgtgcttctg cgcgtaagcc ctcggatctc
     4141 ctcctcgagc ctcgcgatcc tctcggtgaa ctcgaggacc ctttcctgag aaccccctgt
     4201 gttctcgtat gccgccatgt cgcctccgaa cctcgttcag ccgccagggc gaccggacct
     4261 tgagagagta atgccgatta gtcttaataa cgataataat gattactcat acattgtcaa
     4321 cagcgattct caggtttccc gtattcttgc gatcacgtgt gttttcgagg gtgcgctatg
     4381 ggccataggt atcgccggta tcgccttcgg gtgcctgtcc catcccggca tacgcgacgg
     4441 acatcaccat ggggtcgtaa cgggcatctg ccacaggacg gtcgcccggt cggagtacgt
     4501 cgcccttggc tctctcccta tgcccaagcc tctcctcttg cctgccggcc ttaagaaaaa
     4561 ggtacgggaa gggatccgtt cacgaagggg gccgcactcg ccgagatggg agcgttcccc
     4621 ctgggggggt gccgcaggca tcgcgcgcgg gctggaccgc gtgagggtct cggccgggcc
     4681 ggatggttgc ggggaaagca cctcatggaa catggcccag tcatcacgcg tgggctggac
     4741 cgcgtgaggg tgtcggcaag tcctgagggt ttgcggggaa agcactgcat ggaacatgaa
     4801 agggacgggc gggcctatgg ccttgagacg tcacgggagc gtgacgcgac aacgggtcag
     4861 gctgcctatc acccctgcca gaggagcatt tcgtcgatcc tccggtcctt ctgctgccag
     4921 agcatgttga cccattcctg gatgtacttc cggtacgatg cgttgtccgt gtagtcccct
     4981 atgaggtcgc gggtgaccgg gatctgccgg acgtgcaccc ggatttcgtc gacctttcca
     5041 cagaggtagt cccagaacgt gcgtacccct ttggggtaga cgatggtcac gtcgagtatg
     5101 gagtgtatca tctcccccat ggcgcccagg accagcgcca ccccccctgc cttgggcctg
     5161 aggagatgcg tgaacggcga gttctgtctc gcgtgcttgt cctgacggaa cctcgtcccc
     5221 tcgacgaagt tgaagatgga gacgggcatc cccttgaact tctcgcacga cttcttcgcg
     5281 atctcgaggt ccttcccttt gagcttcggc cttcggcgta tctcctcggg cgtgtatctc
     5341 cgcatgaccg ggaaatccac ggcccagaac gcctgcccca cgatcggcag ccagaggagc
     5401 tccctcttca cgaagaactt gaagtcgggg atcttgcgat agaagagccg gatgagcacc
     5461 atgatgtcca cccacgtctg gtggttgacc acgagcatgc atgagcccgg cacatgtacg
     5521 gagtcgagcc ccctgacgag ccatttcgtc tttcgggaca tggcctgcgg caggtcgagg
     5581 aaatagagcc acacgtccgc caccctgtgg agcagccagg tgcagcgcct cctccacgcc
     5641 tccaccggga cgacccactt gagcagcgcc accgtgtaga acgggacggt cagcatgaac
     5701 gtgtcggcga cgaagagcag catggagagg attcccctca gctgtgcacc cacgagtcgc
     5761 agtaatcgta ccatgtcctt ttcctggaat gcgcaaggtg tctttgagaa cgctagggcc
     5821 tgcgagcctt cgatgcctcc c
//
LOCUS       DAOSCR010000086         5807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8762_length_5807_cov_3.762865, whole genome shotgun sequence.
ACCESSION   DAOSCR010000086 DAOSCR010000000
VERSION     DAOSCR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8762_length_5807_cov_3.762865"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLT69_03460"
     CDS             complement(<1..1013)
                     /locus_tag="PLT69_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ13531.1"
                     /translation="MAHILIVEDDINLGQVIFQELKRHNHEVELVENAEAALVRVNKY
                     IYDLMITDLKLPGIDGIELLKKVKATNQGTIVLVMTGYASVDTAVVAMKHGAQDFIQK
                     PFGLSELVQKIDDALSIKRMRNEIDYLRHAQEDIIYRTSDIVGVSSALKKVLKMAEKV
                     AKADSTLLITGETGVGKGLIAGAIHHNSSRASNNFVQVNCAALPHNILESELFGHEKG
                     AFTGAVRTRVGRVEQANMGTIFLDEIGDMDIGLQAKILRVLEDREFERVGGEKTIKVD
                     VRVIAATNQDLFSLVKQGRFREDLYYRINVVNIHIPPIRQRKEDIEPLANYFARKYCR
                     EFN"
     gene            1181..1816
                     /locus_tag="PLT69_03465"
     CDS             1181..1816
                     /locus_tag="PLT69_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ13532.1"
                     /translation="MFLKTVVVTDFMTNCFILGDEHVGRCFIIDPGGEAGKIMKQVEA
                     AGMEVAAVIMTHAHVDHIGAVREIKAETGAPVMLHRLELPLYQAASRMGRLFGIRVEQ
                     PGDPDSFVAEGDEISLGNSLGLRVIETPGHSPGGISLVTADGKTCFAGDTLFAGSIGR
                     TDLPGGDYRTLLDSIKRRLLPLGDDVRVLPGHGPATTLGAERRYNPFLVGG"
     gene            1819..2241
                     /locus_tag="PLT69_03470"
     CDS             1819..2241
                     /locus_tag="PLT69_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13533.1"
                     /translation="MRRTLPAIVAVILLLSGCAQMKSMGLGAEKPAGEEARPAPRFLD
                     FSDVLVPGELQRVNKDCFISNGFGRLVVSGRVQAESLAQFFLTSMNQDGWVSLNQYKF
                     QGSTKLFFKKPDRFATILVTEDPLSTRVEIWVIPQEKI"
     gene            2214..3428
                     /gene="coaBC"
                     /locus_tag="PLT69_03475"
     CDS             2214..3428
                     /gene="coaBC"
                     /locus_tag="PLT69_03475"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOJ13534.1"
                     /translation="MGHTAGEDLEGKTVVYGVTGGIAAYKAPLVARGLKALGVDVHAV
                     MTHAALHFVTPLVMRTLTGNEVHVEMFPETMGSSFEIDHISLADRADLFLVAPATADF
                     IAKLACGMADDLLSCSILATRAPVLVCPSMNVNMLQNRVTQENLDRLKQRGFHVLEPT
                     TGELACGWEGAGRLPEPGRIVQEVRMLMSEHDLSGIRVLITSGPTCEDIDPVRFITNR
                     STGKMGAALVEAACIRGARVKVVSGPVAVDYAPWAEITSVRSAREMLDAVKDCLGEVD
                     VLIMAAAVADYTPHTYSTRKIKKGEKTAHIELTSTPDILATIRPFKESKVFVGFAAET
                     DNLKTYAQDKMVRKGLDMIVANRVGDADSGFASDTNSGVILSPEGVMYDFENMGKDLL
                     AHRILDLVKERL"
     gene            3425..4114
                     /locus_tag="PLT69_03480"
     CDS             3425..4114
                     /locus_tag="PLT69_03480"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ13535.1"
                     /translation="MREHIVQLLMDELRWGGPFLVLPQNSGDPSSEHPAAGLDLESLE
                     LRVRTCRACRLAATRKNTVFGEGDPRARLMFIGEGPGAVEDDTGRPFVGPAGELLTRI
                     IAAMGLDRKDVYIANVVKCRPPGNRDPEPDEIRACMGYLEDQIRLVGPQVIVALGRVA
                     SHALLDETTPIGRLRGTFRRYGDIPLMPTYHPSYLLQNEARKKDVWEDMKKVMYVLGL
                     KRVQRGSPGRS"
     gene            4153..5394
                     /locus_tag="PLT69_03485"
     CDS             4153..5394
                     /locus_tag="PLT69_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOJ13536.1"
                     /translation="MVLDVKDAITPPVAQYLVSGIDEAIETNRAAVIIRLDTPGGLDD
                     AMRTIIQKMMGARVPVIVYVYPKGARAASAGALITLASDVAAMAPGTNIGAAHPVSVG
                     GGDGGGDGEGKTMTEKVTNDAVAYARSIARERGRDEGWAEDIVRKSVSTPAREALEKG
                     VVDIVAEDMQDLLAKLDGRKIKKNSSVFTLKTKDAVLVERPMGFRERFLAAIANPNVA
                     YILMLIGLAGIYFEFSSPGAVLPGVVGGICLILAFFAFQSLPVNYAGFGLILLAAVMF
                     IAEIKVPSYGLLTVGGIVSMVLGALLLFRDPQISAQVSFGVILPVTVVFAAFIALTVF
                     LVVKAHRSAPRSGVESLVGETARVAVWNADGTGKVFCHGEYWDAVGPASLRPDDRVVV
                     TRVRGLNLEVSERATTGGDMP"
     gene            5391..>5807
                     /locus_tag="PLT69_03490"
     CDS             5391..>5807
                     /locus_tag="PLT69_03490"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOJ13537.1"
                     /translation="MIAVALSGGADSTAAAVLLHESGERIFGLTLLLDGDVPPPANVE
                     AARALCRRLGVGHRVVDARGAFEQVKDYFCREYLSGRTPNPCVECNREIKFGLLLRLA
                     LSEGADALATGHYASRKTGLGRFFVSRAAETKSQEYF"
BASE COUNT         1040 a         1786 c         1961 g         1020 t
ORIGIN      
        1 ttgttgaact ccctgcagta cttcctcgcg aagtagttgg ccagaggctc gatgtcttcc
       61 ttgcgctggc ggatgggcgg gatgtggatg ttgaccacgt tgatgcggta gtagaggtcc
      121 tcgcggaacc tgccctgctt gaccagcgag aagagatcct ggttcgtcgc ggcgatcacc
      181 ctgacatcga ccttgatcgt cttttcgccc ccgacgcgct cgaactccct gtcctccagg
      241 accctgagga tcttcgcctg caggccgatg tccatgtccc cgatctcgtc gaggaagatg
      301 gtgcccatgt tcgcctgctc gaccctgccc accctcgtgc ggacggcgcc ggtgaaagcg
      361 cccttctcgt gcccgaagag ctcgctctcg aggatgttgt gcgggagcgc cgcgcagttg
      421 acctggacga agttgttgga agcgcgtgac gaattgtggt ggatggcgcc ggcgatgagg
      481 cccttcccga ccccggtctc gcccgtgatg aggagggtgg agtccgcctt cgccaccttc
      541 tccgccatct tgaggacctt cttgagcgcc gagctcacac ccacgatgtc gctggtgcga
      601 tagataatgt cttcctgggc gtggcgcagg tagtcgatct cgttccgcat gcgcttgatc
      661 gagagggcgt cgtcgatctt ctgcacgagc tcgctgagcc cgaagggctt ctggatgaaa
      721 tcctgcgccc cgtgcttcat ggccaccacc gccgtgtcca ccgaggcgta cccggtcatg
      781 acgagcacga tggtgccctg gttcgtggct ttcaccttct tgagcagctc gatgccgtct
      841 atgccgggga gcttcaggtc ggtgatcatg aggtcgtaga tgtacttgtt caccctcacc
      901 agggcggctt cagcgttttc gaccagctcg acctcgtggt tgtgacgctt cagctcctgg
      961 aagatgacct gccccaggtt gatgtcgtct tcgactatga gtatgtgtgc catgggtgat
     1021 gtagtgatcg gcaggtcgca ccgcaaactt tacaattctg taatcatctc ccggcgcgga
     1081 gaaagccctc gctgttgacc tgatcaaggc ggtatactat ggtgaagccg ggcccgctcg
     1141 ggagtgcctc gcgggctgtg cgaaggagga aaaggcgaga atgtttctca agacggtagt
     1201 ggtcaccgac ttcatgacca actgcttcat cctgggggat gagcacgtgg ggagatgctt
     1261 catcatcgac ccgggcgggg aagccgggaa gatcatgaag caggtggagg ccgccggcat
     1321 ggaggtggcc gcggtgatca tgacgcatgc acacgtggac cacatagggg ccgtccgcga
     1381 gatcaaggcc gaaaccggcg caccggtgat gctccacagg ctggagctgc ccctctacca
     1441 ggccgcctct cgcatgggga ggctcttcgg catccgcgtc gagcagccgg gcgaccctga
     1501 cagcttcgtg gccgagggcg acgagatatc cctcggcaat tccctgggac tcagggtcat
     1561 agagacgccg ggacactcgc ccggcggcat atccctggtc accgccgacg ggaagacgtg
     1621 ctttgcgggt gacacgctgt tcgccggatc catcggcagg accgacctgc cggggggcga
     1681 ctaccggacg ctgctcgact ccatcaagag gcggcttttg ccgctgggcg acgacgtgag
     1741 ggtgctgccc ggtcacggtc ccgccaccac gctcggcgcc gaacgaaggt acaatccatt
     1801 cctggtggga ggttgaagat gcgcagaaca ctaccggcga tcgttgcggt gatactcctg
     1861 ctttccgggt gcgcccagat gaagtccatg gggctgggcg cggaaaagcc ggcgggcgaa
     1921 gaggccaggc cggcgccgcg gttcctcgac ttttccgacg tcctcgttcc cggcgagctc
     1981 cagcgggtca acaaggactg cttcatctcg aacgggttcg gaaggctcgt cgtctcagga
     2041 agggtccagg ccgagtccct cgcccagttc ttcctcacct ccatgaacca ggacggctgg
     2101 gtctccctca accagtacaa gttccagggt tccaccaagc tcttcttcaa gaagccggac
     2161 aggttcgcca ccatcctcgt gaccgaggat cccttgtcga cgcgggtgga gatatgggtc
     2221 ataccgcagg agaagatcta gaaggcaaga cggtcgtcta cggcgtcacc ggcgggatag
     2281 ccgcgtacaa ggccccgctt gtggcgaggg gcctgaaggc cctcggcgtg gacgtgcacg
     2341 ccgtgatgac ccacgccgcg ctccacttcg tcacgcccct cgtgatgaga accctcacgg
     2401 gaaacgaggt ccacgtggag atgttcccgg agaccatggg ttcgagcttc gagatcgacc
     2461 acatatcgct ggccgacagg gccgacctct tcctcgtcgc gccggcgacg gcggacttca
     2521 tagcgaagct cgcatgcggc atggcggacg atttgctgtc atgctcgatc ctcgcgacca
     2581 gggcgcccgt gctcgtgtgc ccgtccatga acgtgaacat gctccagaac agggtcaccc
     2641 aggaaaacct ggataggctg aagcagaggg gtttccacgt cctcgagccg acgacgggcg
     2701 agctcgcgtg cggctgggaa ggcgcgggga ggctgccgga gcccggacgg atagtccagg
     2761 aggtgaggat gctcatgagc gaacacgacc tctcgggcat cagggtgctg atcacctccg
     2821 gccccacctg cgaggacatc gatccggtga gattcatcac gaaccgctcg accgggaaga
     2881 tgggcgccgc cctggtggag gcggcgtgca tcaggggcgc gcgcgtcaag gtggtaagcg
     2941 gccccgtggc cgtggactac gccccgtggg cggaaatcac cagcgtgaga tcggcgcgcg
     3001 agatgctgga cgcggtcaag gactgtctcg gcgaggtgga tgtgctcatc atggctgccg
     3061 ccgtcgccga ctacactccg cacacctact cgaccaggaa gatcaagaag ggggagaaga
     3121 cggcccacat cgagctcacc tcgaccccgg acatcctcgc caccataaga cccttcaagg
     3181 agtcaaaggt attcgtgggg ttcgcggcgg agaccgacaa cctcaagacc tacgcacagg
     3241 acaagatggt gaggaagggc ctcgacatga tcgtggcgaa ccgtgtcggg gacgcggaca
     3301 gcgggttcgc ctcggacacc aacagcgggg tcatcctctc tcccgaaggc gtgatgtacg
     3361 acttcgagaa catgggaaag gacctccttg cccacaggat actcgatctc gtgaaagaga
     3421 ggctgtgagg gaacacatcg ttcagctcct catggacgaa ctgagatggg gcgggccctt
     3481 cctcgtactg ccgcaaaaca gcggagaccc ttcctcggaa cacccggccg ccgggctcga
     3541 ccttgagagc ctcgagctcc gggtgcgcac ctgcagggcc tgccggttgg cggcgacgag
     3601 gaagaacacg gtcttcggcg agggggaccc gagggcccgg ctcatgttca taggggaagg
     3661 gcctggtgcg gtcgaggacg acacgggcag gcctttcgtg ggccctgccg gtgaactcct
     3721 caccaggatc atagcggcca tggggctcga ccggaaggac gtctacatcg cgaacgtggt
     3781 caagtgccgc ccgcccggca acagggatcc cgaaccggac gagatccgcg catgcatggg
     3841 ttacctggaa gaccagatcc ggctggtcgg cccgcaggtc atcgtggccc tgggcagggt
     3901 ggcctcccac gccctcctgg acgagaccac ccccatcggc aggctcaggg ggaccttccg
     3961 caggtacggg gatatcccgc tgatgccgac ctaccaccca agctacctcc tccagaacga
     4021 ggcgagaaag aaggacgtat gggaagacat gaagaaggtc atgtacgtgc tgggattgaa
     4081 gcgcgtgcag cgtggttcgc ctgggcggtc gtagccctcc tcgcgccgag cctctccctt
     4141 gcggcggagg tgatggtcct cgacgtcaag gacgccatca ccccccccgt ggcgcagtac
     4201 ctggtgtcgg gcatcgacga ggccatcgag acgaacaggg ccgcggtgat catacggctc
     4261 gacacccccg gcggtctcga cgacgcgatg cggacgatca tccagaagat gatgggggcg
     4321 agagtgccgg tgatcgtgta cgtgtacccc aagggcgccc gcgcggcttc cgcgggggcg
     4381 ctcatcaccc tggcctctga cgtggcggcc atggcgccgg gcacgaacat aggggcggcg
     4441 cacccggtga gcgtgggggg aggtgacggc ggcggcgatg gcgagggaaa gaccatgacc
     4501 gagaaggtca cgaacgacgc cgtcgcctac gcccgcagca tagccaggga gaggggcagg
     4561 gacgaggggt gggccgagga tatcgtgcga aagagcgtct cgacgcccgc acgcgaggcc
     4621 ctcgagaagg gagtggtcga catcgttgcc gaagacatgc aggacctcct cgccaagctg
     4681 gacggcagga agatcaagaa gaactcctcg gtcttcaccc tgaagacgaa ggacgcggtg
     4741 ctcgtcgagc ggccgatggg tttcagggag cgctttctcg cggcgatagc gaaccccaac
     4801 gtggcctaca tcctcatgct catcgggctc gcagggatct acttcgagtt ctcgagcccg
     4861 ggggccgtgc tgccgggcgt ggtcggcggg atctgcctga tcctcgcctt cttcgccttc
     4921 cagtccctgc ccgtgaacta cgctggcttc ggtctcatcc tgctcgcagc ggtgatgttc
     4981 atcgcggaga tcaaggtgcc gagctacggc ctcctgacgg tggggggcat cgtgtcgatg
     5041 gtgctcggcg ccctgctgct cttcagggac ccacagatct ccgcccaggt ctcgttcggc
     5101 gtcatccttc ccgtgacggt cgtgttcgcg gcgttcatcg cgctgacggt cttcctcgtg
     5161 gtgaaggcgc acaggtcagc cccgcgcagc ggggtcgaga gcctcgtcgg ggagaccgca
     5221 cgcgtggccg tgtggaacgc ggacgggacc gggaaggtct tctgccacgg tgagtactgg
     5281 gatgcagtgg gaccggcgtc gctgaggccg gacgacaggg tcgtggtgac gagggtgagg
     5341 gggctgaacc tcgaggtgag cgaacgcgcc accaccggcg gtgacatgcc atgatcgccg
     5401 tcgccctgtc ggggggggcg gattcgacgg ccgcggcggt gctcctccac gagtccggcg
     5461 agcgcatctt cggcctgacg ctcctcttgg acggcgacgt cccgccgccc gcaaacgtcg
     5521 aggcggcgcg ggccttgtgc cggcgcctgg gcgtaggcca tcgcgtggtg gatgcgcgcg
     5581 gggcgttcga acaggtcaag gattacttct gcagggagta cctttccggg aggaccccga
     5641 acccgtgcgt cgagtgcaac agggagataa agttcggcct gctcttgagg ctcgcgctct
     5701 cggagggggc agacgccctg gcgacggggc attacgcctc ccgcaagaca ggcctgggca
     5761 ggttcttcgt ctcgcgtgcc gccgagacga agtcccagga gtacttt
//
LOCUS       DAOSCR010000087         5804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8773_length_5804_cov_2.842407, whole genome shotgun sequence.
ACCESSION   DAOSCR010000087 DAOSCR010000000
VERSION     DAOSCR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_5804_cov_2.842407"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..1408
                     /gene="nadE"
                     /locus_tag="PLT69_03495"
     CDS             440..1408
                     /gene="nadE"
                     /locus_tag="PLT69_03495"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ13538.1"
                     /translation="MSFGVHSLDIDVVREAERIVEFLGHCVHTVYRRSGIVVGMSGGI
                     DSAVMAELSWRAIGKENVLGLVLPERESNPSSSEYAVAHAKKMGIEYRVKDITKTVES
                     VMSYAERDAYVKSLVPEYTEGSMYHIALPTDLLERDSFNVYVLKVETPDGTIHKKRLG
                     TQEFRRVTSFANIKIRARMLHLYHEAERLGYVVAGTTNRTEYLLGDFCKYGDGGTDIE
                     ALAHLYKNQVYQLGRHLGVPREILERTPSPDTFSLPVSDQEFFFRIPFDKLDHLLYAW
                     ERGIGVEETAEALGVGLDAVKRAFADFASKNRATAHLRVMPYSLEA"
     gene            complement(1701..2720)
                     /locus_tag="PLT69_03500"
     CDS             complement(1701..2720)
                     /locus_tag="PLT69_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ13539.1"
                     /translation="MKKAWVFLVVTVIVASWLPAFSKDTYYIEVLQVSKINPFDMAYQ
                     GFIDELAKNAIVKGKNLTIHRHVIDADADASIWKKVSILMQIKSKASEIVDKKPDLVL
                     TIGTPATKYSKDKFIAAGIPVVFTAVAVPQAVGCKSLTEPGPGFTGSTLYMDPTKAFK
                     ITKLAFPNLTKMGIVHSDDDNAIAFTEEAKKKASALGITVLNKEVGKSDSPKAAAEEL
                     LGKGVQAFGIPIDAYYALRNYGPLKELLSITDSRKIPVISFCYVGFEGGLLYIGSEFN
                     YIGSLAGRQASQILKQGVKPETLPVVMQEDLTILVDLDTMKKLGITLPMEILRIAKSV
                     ESAKK"
     gene            complement(3240..3731)
                     /locus_tag="PLT69_03505"
     CDS             complement(3240..3731)
                     /locus_tag="PLT69_03505"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOJ13540.1"
                     /translation="MIGYRAPTYSITRKTSWAIPILEEAGFRYDSSIFPIYHDTYGIP
                     EAPRFPHRIEGTSLVEFPMSTLRVCRMNLPVCGGGYFRLLPYTATRFALRSIERSGMP
                     FIFYLHPWEIDPDTPRVAGMQPLARFRTYTGIPGARAKFALLLKDFAFAPVKDVLAGM
                     GLL"
     gene            complement(3789..4073)
                     /locus_tag="PLT69_03510"
     CDS             complement(3789..4073)
                     /locus_tag="PLT69_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ13541.1"
                     /translation="MINALSIDVEEYYQINALSGVLDMGMWDSAPSTVERNTSLVLDI
                     LDSEDVKATFFCLGWVARRNAGIVRRIHDRGHEVACHGYSHILVNRQSPG"
     gene            4643..5608
                     /locus_tag="PLT69_03515"
     CDS             4643..5608
                     /locus_tag="PLT69_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13542.1"
                     /translation="MAYSEKHVPGIFHNRAERYQYETFVRYKHAGKYTAVSWKEMQNR
                     VIKTGLGLISLGCEPRDMIGIFSENCWEWIVADLAALSIGCTDVPIYATNSGAEAAYI
                     INDSGTKVLFVSDKEHLDKILSVRKRLDSLTKIVTFEKIEGAPADVMGLDELMALGEA
                     FADRDAFERRLNAIDPEDLATLIYTSGTTGQPKGVMLTHANFVANLMQSYASHPIIGH
                     DDEALSFLPWSHSLGRTVSLYLMIHIGAVISLAESFSTVLENMQETRPTLMVSVPRLF
                     EKIHAGILAKADKAPPLKKKLFEWALGVADRRWLTWCSARPCPGS"
     gene            5566..>5804
                     /locus_tag="PLT69_03520"
     CDS             5566..>5804
                     /locus_tag="PLT69_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13543.1"
                     /translation="MAYVVQRKAMPGVLKTQYDIADALVFKKLREALGLDRIRIFISG
                     GGPLSTDIDRFFNGIGIPVHNGYGLTETSPVTNVN"
BASE COUNT         1099 a         1762 c         1874 g         1069 t
ORIGIN      
        1 aggccgttat cttctctgcg agatctgtca tcgccacccc ttcgatcgtt ggacgctcga
       61 tgttcctatc ggatttcact atcgcagtca tgagaaatgg gcaatggcaa agatacacgg
      121 gacgaactca cctaccggag aacttcccag actggagggc tcttcctcga gcacatccgg
      181 cccgttgtcg agagccccgc ctcccggcag ggaaacgtat ctgggatgca acccgaggga
      241 agatggtctc ccgtgtgagt ccttcgccct ggaggaggat gttcatgccc tatcaccatc
      301 ggcccccacg atcagctcga agcaggggtc tggtgatcat tgggcgcccg gagcccgtgg
      361 ccgtgacctt ggcggttgtg gtggctctcg agaggatgta gtatggccgt gatcaacagg
      421 aaagaagacg ggaggcggta tgtcctttgg tgtccattcg ctcgacatcg acgtcgttcg
      481 ggaggcggag cgtatcgtgg agttcctggg ccattgtgtc cacacggtgt acaggaggtc
      541 agggatcgtg gttgggatga gcggcggcat cgactcagcc gtcatggccg agctctcctg
      601 gagggccatc ggaaaggaaa acgtgctcgg ccttgtcctg cccgagcgcg agtcgaaccc
      661 gtcgagcagc gagtatgccg tcgcccatgc gaagaagatg ggcatcgagt acagggtcaa
      721 ggacatcacc aagaccgtgg agagcgtcat gtcctacgcc gagagggacg cgtacgtgaa
      781 gtcgctcgtc cccgagtaca cggaaggctc catgtaccac atagcgctgc ccacagacct
      841 gctcgagagg gactcgttca acgtctacgt gctcaaggtc gagaccccgg acgggacgat
      901 ccacaagaag agactgggca cccaggaatt caggagggtg acctcgtttg cgaacatcaa
      961 gatcagggcg cgcatgctgc acctctacca cgaggctgag aggctcgggt acgtggtcgc
     1021 tggcaccacg aacaggaccg agtacctgct cggggacttc tgcaagtacg gcgacggagg
     1081 caccgacatc gaggcgctgg ctcacctcta caagaaccag gtctaccagc tgggacgcca
     1141 cctgggcgtg ccccgggaga tcctggagag gacgccgagc cctgacacct tcagcctccc
     1201 ggtgagcgac caggagttct tcttcaggat cccgttcgac aagctcgatc acctcctgta
     1261 cgcatgggag cgcggtatcg gcgtggagga gacggccgag gcgctcggcg tgggcctgga
     1321 tgcggtcaag cgcgcattcg ccgacttcgc ctccaagaac agggcgacgg cgcacctgcg
     1381 cgtcatgccc tacagcctgg aagcctgagc cccaccagag acccagcaca atcgacggtc
     1441 ttcggagagc ccgtggccgt cagccttggg cgccaggaaa cccccaccct ggatccatga
     1501 gaggacatca tcgatcgtga agggacaccg gacggagagc ccgtcttgaa cccccggcac
     1561 taccgggcag gggccataca ccatcgagga gattcgggtg ccggcggcct cgggcacccc
     1621 tttcgatgtc cttgacggta ccatgcccgg cacctttgag aaggggcggg ttccgggggc
     1681 cttaggcccc ctcgagccgg tcacttcttc gcggactcga cgctcttggc gatgcgcagt
     1741 atctccatgg ggagcgtgat cccgagcttc ttcatggtgt cgaggtcgac caggatcgtg
     1801 aggtcttcct gcatcacgac aggcagcgtc tcgggcttga ccccctgctt caggatctgc
     1861 gaggcctgcc tgcctgcgag tgagccgatg tagttgaact cggacccgat gtagaggagc
     1921 ccgccttcga agccgacata gcagaagctg atgacgggga tcttcctgga gtcggtgatc
     1981 gagagcagct ctttcaaggg gccgtagttc ctgagcgcgt aatacgcgtc tatggggatg
     2041 ccgaaggcct ggacgccctt cccgaggagc tcctcggccg cggccttggg gctgtccgac
     2101 ttgccgacct ccttgttcaa cacggtgatc ccgagggcag atgccttctt cttcgcctcc
     2161 tcggtgaatg cgatggcatt gtcgtcgtcg ctgtggacga tccccatctt cgtgagattg
     2221 ggaaaggcaa gcttggtgat cttgaaggcc ttggtcgggt ccatgtacag cgtggatccc
     2281 gtgaagcccg gcccgggctc ggtgagcgac ttgcagccca cggcctgggg caccgcgacg
     2341 gcggtgaaca ccaccgggat gcctgcagcg atgaacttgt ccttgctgta tttggtggcc
     2401 ggggtgccga tggtgagcac gaggtccggt ttcttgtcca cgatctcgga agccttgctc
     2461 ttgatctgca tgaggatcga gaccttcttc cagatgctcg cgtccgcatc cgcgtcgatg
     2521 acatgcctgt gtatcgtgag gttcttgccc ttgacgatgg cgttcttggc aagctcgtcg
     2581 atgaagccct ggtaggccat gtcgaacggg ttgatcttcg agacctggag gacctctatg
     2641 tagtaggtgt ccttcgagaa agcgggaagc cagcttgcga cgatgacggt tacgacgagg
     2701 aagacccatg ccttcttcat gtgcagtacc tcctcaaata tagagtgaat gctcacatgc
     2761 aggtgatgct ccgtgctgac cgggagcata ggaccgtggg ccgtgcttgt caagcatggc
     2821 tgcggttcgt tgaccggaca cccccgtgcc gtggcgggca tgacggccat gggggcgatc
     2881 accgtctccc atgacgtcgc cttccctggt gttctgtgcc gcccggtgag cgaggcggcg
     2941 gtcgggggcc atgcgcggca tggaccggta cgtgggcctg ccgaggaaga ggtctgtgtc
     3001 tcaggaggtg ctgccatggc ggatcgaggg ctcgacaagg ggtatcctgg gaaagggcag
     3061 agccgtttct tgggggcatc cctgaaacct gacgcacgga ctcgaggccg tgccgcgcaa
     3121 gagggcacct tccaggccgt cgtggtcctc gccgcagtcg ccccggtgca ccccggcccc
     3181 ttgggtgaac cccttgcgat tgacggtgag gcccctgtgc atcttccccg ggaccgggtt
     3241 cacaacagtc ccatgcccgc cagcacgtcc ttgacgggtg cgaacgcgaa gtctttgagc
     3301 aggagagcga acttcgccct cgctcccggg atgcccgtgt atgtcctgaa gcgcgcaagg
     3361 ggctgcatgc ccgccaccct cggggtgtcg gggtcgattt cccaggggtg caggtagaag
     3421 atgaacggca tgccgcttcg ctcgatcgac cggagggcga acctcgtcgc cgtgtagggg
     3481 agcagcctga agtacccgcc tccgcacacg ggcaggttca tgcggcagac cctcagggtg
     3541 gacatgggga actccaccag cgaggtgccc tcgatcctgt gggggaacct cggcgcctct
     3601 ggtatgccgt aggtgtcgtg gtagatgggg aagatgctcg agtcgtacct gaaacccgct
     3661 tcctcgagaa tggggatcgc ccacgacgtc ttgcgggtga tcgagtaggt gggcgccctg
     3721 tagcctatca cgcgctcgcc gatggtgtcc tcgatgagcg acttcgcggt cttgacgtcc
     3781 tgctcgaact agccagggct ctgcctgttc acgaggatgt gcgagtaccc gtggcaggcc
     3841 acctcgtggc ccctgtcgtg gatgcgcctc acgatgccgg cgttccgtct cgccacccag
     3901 ccaaggcaga agaaggtggc cttgacgtcc tcggagtcga ggatatcgag caccagcgag
     3961 gtgttcctct ccaccgtcga cggggccgaa tcccacatcc ccatgtccag gacaccggag
     4021 agggcgttga tctggtagta ttcctcgacg tctatgctga gcgcgttgat cacgtggcaa
     4081 ctatagagcc tttcgggccc gatggcaacg cgttcccgcc cacggggtcg atacggccgg
     4141 gatgccgtgg ggcagcgtcc cgggttgaag gaccgcgttt ccgggtggtc gcggcggcgc
     4201 acgccaggcg gtacgcaacg agcaggccgc cgtgaggccg agagagcgcc ttgccggggg
     4261 ccgaggaaca ggcccgtggg agggcgcggc cgaccggcac gggctggcga cgatctcgaa
     4321 acggcaggaa agcatcaggg cattgcggcg ccgggatgaa cgacgacccg caatccgtgc
     4381 cgcgaggctc atcgcccgac aggcccctcc acgaaaccct gcgtcgtctc gttcccttcg
     4441 agaaggatcc tgcatgagcc cataactcga tccgtggact gaacggccgc acgggccaca
     4501 ccgggcgcgt gttccccttg cccataccat gccttgtccg gaccggaaga aaggtgtaga
     4561 taactctggt tggaccagag gcgctgattc ccgtcatcac gagaagagac acacggcatc
     4621 caagaaccga aaggagggaa gcatggcgta ttccgagaaa cacgttccgg gtattttcca
     4681 caacagggca gagcgttacc agtacgagac cttcgtcagg tacaagcatg cggggaagta
     4741 caccgccgtc agctggaagg agatgcagaa cagggtgatc aagaccgggc tcgggctcat
     4801 ctccctcggt tgcgagccca gggacatgat cggcatcttc tccgagaact gctgggaatg
     4861 gatcgtcgcc gaccttgccg cactctcgat cgggtgtacc gatgtgccca tttacgcgac
     4921 caactccggc gcggaggccg cctacatcat caacgattca gggacaaagg tgctcttcgt
     4981 ctcggacaag gagcatctcg acaagatcct ctcggtgaga aagcgcctgg actctctcac
     5041 gaagatcgtg accttcgaga agatcgaggg tgcacctgcg gatgtgatgg ggctcgacga
     5101 gctcatggcg ctgggggagg ccttcgccga cagggatgcc ttcgagagga ggttgaacgc
     5161 gatagacccc gaagacctcg caacgctcat ctacacctcc ggcacgaccg gccagcccaa
     5221 gggggtcatg ctgacccacg cgaacttcgt ggcgaacctc atgcagtcct acgcgtcgca
     5281 ccccatcatc gggcacgacg acgaggcgtt atccttcctg ccgtggagcc actccctcgg
     5341 caggacggtc tctctctacc tcatgatcca tatcggcgcg gtgatcagcc tcgccgagtc
     5401 gttctccacg gtcctcgaaa acatgcagga gacccggccg accctcatgg tgagcgtgcc
     5461 caggctcttc gaaaagatac acgccgggat cctcgccaag gcggacaagg cgccccctct
     5521 caagaagaag ctcttcgagt gggccctggg ggtggccgac aggcggtggc ttacgtggtg
     5581 cagcgcaagg ccatgcccgg ggtcctgaag acccagtacg acatcgcgga tgcgctcgtc
     5641 ttcaaaaagc tgagggaggc gttgggcctc gaccggatca ggatcttcat cagcggcggc
     5701 ggccccttgt ccacggatat cgacaggttc ttcaacggca tcggtatacc cgtgcacaac
     5761 gggtacggcc tcaccgagac cagcccggtc accaacgtga acag
//
LOCUS       DAOSCR010000088         5776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8821_length_5776_cov_3.767174, whole genome shotgun sequence.
ACCESSION   DAOSCR010000088 DAOSCR010000000
VERSION     DAOSCR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8821_length_5776_cov_3.767174"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLT69_03525"
     CDS             complement(<1..228)
                     /locus_tag="PLT69_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ13544.1"
                     /translation="MITAGIDIGSITAKAAVVEDGNVLGTKVIFTGYNVETAARKVYG
                     ELLAELGLPADAVERVVATGYGRTGVPFADKA"
     gene            complement(310..1080)
                     /locus_tag="PLT69_03530"
     CDS             complement(310..1080)
                     /locus_tag="PLT69_03530"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ13545.1"
                     /translation="MTGGSIRYGIEGRVFLVTGASRGIGLAIARELVEQGVSLAICAR
                     KQEGLDEAVKLLGGREKVLAIQAHIAKEEDVERMFSEVLGRFGRLDALVNNVGMNLLT
                     PSVADTDTVLWQKILDTNLNGTFLCSRAAARIMRQNKRGKIVTVSSIAGRRASPGMGV
                     YGIAKAAVEMLTRVLAAELAPFNIQVNAVAPSMVRTEFSRPFWSDPAIYEQVARAIPM
                     GRIAETTDVVHPVLFFTSDGSDFITGQTLLVDGGASAV"
     gene            complement(1097..2338)
                     /locus_tag="PLT69_03535"
     CDS             complement(1097..2338)
                     /locus_tag="PLT69_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOJ13546.1"
                     /translation="MSAADSEKPRKFRAAKRMREIMTEYYIEAKGAGSTGKKVAWITS
                     GGPVEPLIAMDVIPVYPENYGAMIGASHMGGDLCAKAEAMGYSTDLCSYARADIACAR
                     ENGGPIGGLPKPDMLICCNNICGTVLKWYETHARYFDVPLFIFDTPFCHTEFPDIARK
                     YVHRQLDEYVAFLEEACGRPFDLEKSSEVGRRSLEGQRLWQEVLDTAAHRPAPMTAFD
                     AFFFLALIVTLRGTQVVVDFYRELLSEMRERIEQGVWAIPNERFRLLWDNLPVWYRLK
                     WLSDTFASHDACLVADTYTTAWCGSMKYIDENDFFGSMAEAYTRIYLNIGVDEMAKTI
                     FSMIDKYQVDGLVMHSNRSCKPYSFGQYDIMRMVQEEKGIPCMIIESHMTDERSFSES
                     QTLTRIEAFMEMIRQRKGLRS"
     gene            complement(2418..3533)
                     /locus_tag="PLT69_03540"
     CDS             complement(2418..3533)
                     /locus_tag="PLT69_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ13547.1"
                     /translation="MERIVEEFKKASADPIAYGRALKEDGRKIIAFFCTYTPEEIIHA
                     SGVHPMRLFGTTRDSSAADRHLQAYCCSLVRGALAQALSGELDFIDGAVFPHTCDSIQ
                     RLSDIWRLNTAYGFFADVVLPVKLTSASARSYFEDVLAKLRSDLEKGLSTRISDDDLR
                     ASIRTYNSIRASLSELYGFVSRGASPLLPGDVSALVRGSMILERSAAERLLKELVRAH
                     DGIGRKDTNLKRVMLVGGLCDHPDIYRLLLDSGALVVWDDLCSGSRYFEGAIPEDGDP
                     IANLAGRYFERAVCPAKHSSTTARARRLVEAAIRERVQGVIFLQLKFCDPHAFDYPYL
                     KETLDSHSIPSMLLEIEDQLPPEGQLLTRFETFVQTI"
     gene            4022..>5776
                     /locus_tag="PLT69_03545"
     CDS             4022..>5776
                     /locus_tag="PLT69_03545"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOJ13548.1"
                     /translation="MGKAKRSFWSFVFVFAVMLVQATMAFAGEADIKLPDLSSVGFNV
                     LGAPVSGLAILNVGLVVCLIGLAFGLLQYSQTKALPAHKQMLSVSNTIWETCKTYLTQ
                     QGKFLLALWILIAICIIYYFGALSHMPAGSIVVILLASVLGILGSYGVAWFGIRINTI
                     ANSRAAFASLQGNPLNIVNICLRSGMSVGLLLVSVELFFMILILAYIPKELAGACFIG
                     FAIGESLGASALRICGGIFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGDS
                     VGPTADGFETYGVTGVALIAFLALALADNPAMGAKLIVWIFAMRILMILTSLVSYFLN
                     LGISSAAFGAKKDFDFEHPLTNLVWITSAVSIAVTFLASYVLLADLDPVLYGDLWWAL
                     AVIISCGTVAGALIPEFTKVFTSTKSRHCEEVVNSSRQGGPSLNILSGFVAGNYSAFW
                     EGLAILVLMLIAYLVSKNPSIHNIMPPEFVFATPVFAFGLVAFGFLGMGPVTIAVDSF
                     GPVSDNAQSIYELSMIESVPNVKEEIKKDFGFDPDFEHSKHYLESCDGAGNTFKATAK
                     PVLIGTAVVGATTMVFGII"
BASE COUNT          992 a         1933 c         1689 g         1162 t
ORIGIN      
        1 ggccttgtca gcgaagggga ccccggtcct gccgtatccg gttgccacga cccgctccac
       61 tgcatcggcc ggcagaccga gctcggcgag gagctctccg tacaccttcc gcgcggcggt
      121 ctccacgttg tatccggtga agatgacctt cgtgccgagg acgttgccgt cttcgaccac
      181 ggccgccttc gcggtgatgg acccgatgtc tatccctgct gtgatcatgt cggtcgcttc
      241 ccccgggtca gtcttgacgg atcggcgatg gtgcccgcac agacgctggc gtgtcccggc
      301 gggccggcct cataccgcgc tcgccccccc gtccacgagc agggtctgcc cggtgatgaa
      361 gtccgatccg tctgatgtga agaagagcac ggggtggacc acgtcggtgg tctcggcgat
      421 gcggcccatg ggtatggccc tcgcgacctg ctcgtagatc gccgggtccg accagaacgg
      481 cctgctgaac tcggtgcgca ccatcgaggg cgcgaccgcg ttgacctgta tgttgaaggg
      541 cgcgagctcg gccgccagga ccctcgtgag catctcgact gccgccttcg cgatcccgta
      601 cacccccatc cccggcgacg ccctgcggcc ggctatggat gacaccgtga cgatcttgcc
      661 ccgcttgttc tgccgcatga tcctggcggc agccctcgaa cagagaaagg tgccgttcag
      721 gttcgtgtcc aggatcttct gccacaagac ggtgtcggtg tcggccaccg acggggtcaa
      781 caggttcatg cccacgttgt tcacgagcgc atccagcctg ccgaaccggc cgaggacctc
      841 ggagaacata cgctcgacat cctcttcctt cgcgatatgg gcctggatcg ccagtacctt
      901 ttcccggccc ccgaggagct tgaccgcctc gtcgaggcct tcttgcttcc ttgcgcagat
      961 cgcgaggctc accccctgct ccacgagctc cctggctatg gcgagaccga tgcccctgct
     1021 cgcccccgta accaagaaca cccttccctc gattccgtac ctgatggatc cacccgtcat
     1081 gggaaacctc ccgtcctcag cttcgcaatc cctttctctg gcggatcatc tccatgaatg
     1141 cctcgatcct ggtgagcgtc tggctctcgg agaaactccg ctcgtccgtc atgtgggatt
     1201 cgatgatcat gcacgggatc cccttctctt cctggaccat gcgcatgatg tcgtactgtc
     1261 cgaacgagta cggcttgcag ctcctgttcg agtgcatgac gagaccgtcg acctggtact
     1321 tgtcgatcat ggagaagatg gtcttggcca tctcgtccac gcctatgttc aggtaaatcc
     1381 gcgtgtacgc ctctgccatg gagccgaaga agtcgttctc gtctatgtat ttcatgctcc
     1441 cgcaccaggc ggtcgtgtac gtgtccgcga cgaggcaggc gtcatgggat gcgaacgtgt
     1501 ccgagagcca cttgagcctg taccatacgg gcaggttgtc ccacaggagg cggaagcgct
     1561 cgttcgggat ggcccagacg ccttgctcga tcctctcccg catctccgag agcagctccc
     1621 tgtagaagtc caccaccacc tgggtacccc ggagcgtcac gatgagggcg aggaagaaga
     1681 aggcgtcgaa ggcggtcatg ggggcgggcc tgtgcgcggc cgtgtcgagc acctcctgcc
     1741 agagcctctg gccctcgagg gaacgtctcc ccacctcgga acttttctcg aggtcgaacg
     1801 gacggccgca tgcctcctca aggaaggcca cgtactcgtc gagctgcctg tggacgtatt
     1861 tcctggcgat gtcagggaac tcggtatgac agaagggtgt atcgaaaatg aagagcggga
     1921 catcgaagta gcgggcgtgt gtctcgtacc atttgagcac ggtgccgcag atgttgttgc
     1981 agcagatgag catgtccggt ttgggcagcc cccctatggg gcccccgttc tccctggcgc
     2041 aggcgatgtc ggccctcgcg tacgagcaca aatccgtgct gtaccccatg gcctccgcct
     2101 tcgcgcacag gtcgcctccc atgtgggatg caccgatcat ggccccgtaa ttctcggggt
     2161 agaccgggat cacgtccatg gctatgaggg gctccacggg ccccccgctc gtgatccagg
     2221 ccaccttctt gcctgtcgac cctgcaccct tggcctcgat gtagtactcg gtcatgatct
     2281 cgcgcatgcg cttggcggcg cggaacttcc ggggcttttc actgtcggcg gcgctcatgg
     2341 tctcacctcg tttgggactt tcatcgcttg ctctttccca cctgcctcac gtcgggccgt
     2401 gccggggtgc tcgcatctca tatcgtctgc acgaaggtct cgaacctggt gagaagctgc
     2461 ccctccggcg ggagctggtc ctcgatctcc aggagcatgc tcgggatgga atggctgtcc
     2521 agggtctcct tcaggtaggg gtagtcgaag gcgtgggggt cgcagaactt gagctggagg
     2581 aatatgacac cctggacgcg ttccctgatc gccgcttcca ccaggcggcg cgccctcgcg
     2641 gtcgtggagg agtgcttggc agggcagacg gcccgctcga agtacctgcc cgccaggttt
     2701 gcgatggggt cgccgtcctc ggggatcgcc ccctcgaaat acctggaacc ggagcacagg
     2761 tcgtcccaca cgacgagcgc gcccgaatcc aaaagcagcc ggtagatgtc ggggtggtcg
     2821 cacaggccgc ccacgagcat gaccctcttg aggttcgtgt ctttcctgcc gatgccgtcg
     2881 tgcgcacgga cgagttcctt gaggagcctc tccgccgcgc tcctttccag gatcatggac
     2941 cccctcacca aggcgctcac gtccccgggg agcagggggg aggcccctct cgacacgaac
     3001 ccgtacagct cagacaggga cgcccgtatc gagttgtacg ttctgatgga agcccgcagg
     3061 tcgtcgtccg agatcctcgt cgacaggccc ttctccaggt ccgatctcaa cttcgccagc
     3121 acgtcctcga aataggagcg cgcgcttgcg gacgtgagct tcacgggcag cacgacgtcc
     3181 gcgaagaaac cgtaggccgt gttcagcctc cagatatcgg agagcctctg gatggagtcg
     3241 cacgtgtggg ggaagacggc gccgtcgatg aagtcgagct ctcccgagag ggcctgcgcg
     3301 agggcgccgc ggaccagcga gcagcagtac gcctggaggt gcctgtcggc tgcggaactg
     3361 tccctggtcg taccgaacag gcgcatcggg tggacgccgg atgcgtggat gatctcctcc
     3421 ggcgtgtagg tgcagaagaa ggcgataatc ttccttccat cctccttcag cgcccgaccg
     3481 taggcgatgg ggtctgccga agccttcttg aactcttcca cgatgcgttc catgtcccct
     3541 ccccttctcg gcaacctgcc ctctcacggc gctgtccacg caagacccag gctatcggtc
     3601 gacccaagaa ccaggacacc ttctccaggt cccgaccccg gcagaacggc atggaagcac
     3661 ttataccaag cgcccgcccg gcctttcaac agaaaacgat cacgtcatac cgtatgtccg
     3721 aaacgccgtc atcgggcgtg ttcccaccag gagggaaagg tcacctggaa tcagcggaga
     3781 agccggtcaa tcccctgaaa cgacgggttt tctcggagaa aaaaaacctt gacacgcaaa
     3841 ggcttctatt gtaggttcat gtgggctgaa tgatgattca ctcatgcccc ggaacgtacg
     3901 gtccgccttt cttccacgtg cacgatcctc tcggctcatc gaggttcgcg gctcggggaa
     3961 aaggcggcgg ttccccgggc aacaggctca tccagttatc attacacaag gagacgcgat
     4021 tatgggcaaa gcaaaacgga gtttctggtc cttcgtgttc gtcttcgccg tcatgctcgt
     4081 gcaggcgacg atggcgttcg ccggggaagc tgacatcaag ctccctgatc tctcatcggt
     4141 cggcttcaat gtcctcggcg ccccggtcag cggcctggcg atcctgaacg tcgggctggt
     4201 cgtctgcctg atcggcctgg ccttcggcct cctgcagtac agccagacta aggcccttcc
     4261 ggcgcacaag cagatgctct cggtctccaa caccatctgg gagacctgca agacgtacct
     4321 gacccagcag ggcaagttcc tgctcgcgct gtggatcctc atcgccatat gcatcatcta
     4381 ctatttcggc gctctgagcc acatgccggc aggcagcatc gtcgtgatcc tgctcgcctc
     4441 cgtcctcggc atcctcggtt cgtacggcgt ggcctggttc ggcatcagga tcaacacgat
     4501 agccaactcc cgtgcggcat tcgcctcgct ccagggcaac cccctcaaca tcgtcaacat
     4561 ctgccttcgt tcgggcatga gcgtcggcct gctcctggtg agcgtcgagc tcttcttcat
     4621 gatcctcatc ctcgcctaca tcccgaagga gctcgcgggc gcctgcttca tcggcttcgc
     4681 catcggcgaa tccctgggtg cgagcgcgct ccgtatctgc ggcggtattt tcaccaaaat
     4741 cgccgacatc ggcagcgacc tcatgaagat cgtgtttcac ctccccgagg atgaccccaa
     4801 gaatcccggc gtcatcgcgg actgtacggg cgacaacgcg ggtgacagcg tgggccccac
     4861 ggcagacggc ttcgagacct acggcgtgac cggcgtggcg ctcatcgcat tcctggccct
     4921 tgcgctcgcc gacaacccgg ccatgggcgc caagcttatc gtgtggatct tcgcaatgcg
     4981 tatcctgatg atcctcacct cactggtgtc ctacttcctc aacctcggca tcagctcggc
     5041 ggccttcggc gccaagaaag acttcgactt cgagcacccg ctcaccaacc tcgtgtggat
     5101 cacgtcagcc gtctccatcg cggtgacctt cctcgccagc tacgtgctcc ttgcggacct
     5161 cgacccggtc ctctacggcg acctctggtg ggccctggcc gtgatcatca gctgcggcac
     5221 cgtcgcgggc gccctcatcc ccgagttcac caaggtcttc accagcacca agagccgcca
     5281 ctgcgaagag gtggtgaact cttcccggca gggcgggccg tccctcaaca tcctgtccgg
     5341 tttcgtggcg ggtaactact cggcattctg ggaaggcctc gcgatcctgg tcctcatgct
     5401 gatcgcgtac ctcgtgtcca agaacccctc catccacaac atcatgccac ccgagttcgt
     5461 gttcgcgacc ccggtcttcg ccttcggcct ggtcgccttc ggtttcctcg gcatgggccc
     5521 ggtcaccatc gcggtcgaca gcttcggtcc ggtctcggac aacgcgcagt ccatctacga
     5581 gctctccatg atcgagtcgg tcccgaacgt caaggaagag atcaagaagg atttcggctt
     5641 cgatccggac ttcgagcact ccaagcacta cctcgagtcc tgcgacggcg cgggcaacac
     5701 cttcaaggcc acggccaagc ccgtgctcat cggcaccgcg gtcgtgggtg cgaccaccat
     5761 ggtgttcggc atcatc
//
LOCUS       DAOSCR010000089         5773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8825_length_5773_cov_3.353445, whole genome shotgun sequence.
ACCESSION   DAOSCR010000089 DAOSCR010000000
VERSION     DAOSCR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8825_length_5773_cov_3.353445"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /gene="pheT"
                     /locus_tag="PLT69_03550"
     CDS             <1..1896
                     /gene="pheT"
                     /locus_tag="PLT69_03550"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ13549.1"
                     /translation="WVLDISVTPNRGDCLSVIGIARELSVLFGCPLKTPEFSIQEADI
                     DVGSALSVEIVDTRGCPRYAARFIDGIVIAKSPFTMRRRLFQSGVRAINNVVDITNYV
                     MLEYGQPLHAFDSTLLAGPAIVVRRAREGERFVTLDSVERVLRDTDLLICDREKPVAL
                     AGVMGGENSEVLPGTTSVVLESAYFDPDGIRRTSKSLGLTTEASYRFERGVDPGIQVE
                     AANRAAYLMERHARGRVYRGVIDVNHLQDTRAAITLSAGTLERVLGVQPPAEGEVERI
                     FTALGCEVRRIEGGWSVSAPPFRPDVEREIDLVEEFVRIHGMDKVTPELPAFRPTPGG
                     VSRPSLRDLRRSLAAMGFNEVITYSFISPRWKDFFPGDSLRLVNPISDEMSLMRTSLV
                     PGLLGALERNRRLQTRDVCIFEIGRTFHPVEGAGLPREEERLGVLMSGMRFDQHWSDQ
                     PRQVDFYDLKGLAELLIPGVTVRPSDLPHLESGRQGDLVLDGEVIGCLGAVHPSIRTI
                     IDASEDVFVLETVLGPVIERRWKGLAEIPRFPFTWRDLSLVVDEKIPYADIVREVESL
                     GIGEIRKVAALDVYSGEKLPRGKRGVTIRITYQSLERTLEDAEISAWQERVISRLGES
                     LGITLRG"
     gene            complement(2204..2872)
                     /locus_tag="PLT69_03555"
     CDS             complement(2204..2872)
                     /locus_tag="PLT69_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ13550.1"
                     /translation="MTESHRRAIQARRERERNARIQGILQAAKKVFFSKGYLKTTMDE
                     IALEAQVSKPTIYQYFKTKDELYSALMLPFLEEFGAHFETISRRLERGRYASGSKLVK
                     DLFRAFEKSFEACPDSLRVANTFFQNTDLIGELEESTRLAISSRGRYDFELARRLLAK
                     AMERGLIREMSPYMLSDIIWGMMLGITQVMDMKAARGTKTSRLFKETLRAAERIVTEA
                     IAIG"
     gene            3145..4305
                     /locus_tag="PLT69_03560"
     CDS             3145..4305
                     /locus_tag="PLT69_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13551.1"
                     /translation="MDFDLAPEQRMFRETVYRWAKEEVAPLCEDADLRGAFSPEIWGR
                     LAGFGILGLPFPEEFGGSGADIVTCCLAGEALGHAGVDGGHLLAWGAHTYLCGHNILS
                     FGTDEQKRKYLPRLASGEWIGCMGLTEPGSGSDAASLSTSARRTGSGYVLNGTKTFIT
                     NAPVSDVFVIFANVDKKLKHEGITAFIVEKGTPGLSTGRPFHKMGCRCSATSEVILED
                     CEVPAENVLGGEGMGWIIALSGVEWDRCTLLSPMLGCAMFNLDACARYANERRQFGRP
                     IRSFQAISRRIADMKVFVEALRLAIYRVAWAKDKGRMLNPLQAAVSKVYAGDRGFEMA
                     SEAVQVFGGYGFMHEYPVERNFRDSKLAAIGGGTSDIMRMIVSRIMLMGFGV"
     gene            4318..5463
                     /locus_tag="PLT69_03565"
     CDS             4318..5463
                     /locus_tag="PLT69_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13552.1"
                     /translation="MDYELEPSRVDFLKALGAHLAERVAPRRAQLDSCGPEERARLMR
                     DNIAGLGTHGFLAAMSIGNPPDLVGHYLALEETAKACPATCLSARASAFLCAGAISLF
                     GSPGAKETYLPRLLSGEAVGALAYSEPGSGSDLGALGAVAESDAGVYLVDGEKDIVVN
                     APIAEVFVVLARTPEGPSLLVVEGGARGLEIGPCLDTLGIRGAPVGGVRMRRCEAACL
                     LGREPGMGLEQLERLLSMGAVGAAALCVGIAHACMEISTERAKSRSAFGKPIGMFQDV
                     GFKLADMFTLVDLSRLLALRAAWAFDTGERDAPVRAACAKLFSAEACSKVANWGMQIF
                     AGHGYLAGSPIERLYRDARFGEICERTSEILRLTIANEELDRFAGFA"
     gene            5518..>5773
                     /locus_tag="PLT69_03570"
     CDS             5518..>5773
                     /locus_tag="PLT69_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HOJ13553.1"
                     /translation="MIETKIDTGSEEYRKNYEAMERLVADLKDELHKARFERPAKDIE
                     RVKSQGKMLVRDKLDLLLDRDSPFLEIAPLAARGLYDGKVH"
BASE COUNT          968 a         1827 c         2002 g          976 t
ORIGIN      
        1 tgggtgctcg acataagcgt caccccaaac aggggggact gcctgtcggt catcggcatc
       61 gcgcgtgaac tctcggtgct cttcggatgc cccctcaaga cgccggagtt ctccatacag
      121 gaagccgaca tcgatgtggg gtcggcgctg agcgtcgaga tcgtggacac gagagggtgc
      181 ccgcgctacg ccgcgcgctt catcgacggc atcgtcatcg cgaagagccc cttcaccatg
      241 aggaggaggt tgttccagtc cggggtgagg gccataaaca acgtggtgga catcaccaac
      301 tacgtgatgc tcgagtacgg gcagcctctt cacgccttcg attccaccct gctcgccggg
      361 ccggccatcg tggtgcgcag ggcgagggag ggagagaggt tcgtcaccct ggactcggtg
      421 gagagggtcc ttcgcgacac cgacctcctc atctgcgacc gcgagaaacc ggtcgccctg
      481 gccggcgtca tgggcggaga gaactcggag gtgctgcccg gaaccacgag cgtggtcttg
      541 gagagcgcct acttcgaccc cgacggcatc cgcaggacgt cgaagtccct cgggctgacc
      601 accgaggcct cctaccggtt cgagcgcggg gtggacccgg gcatccaggt ggaggccgcg
      661 aaccgcgccg cgtacctcat ggagagacac gcccgcggca gggtgtacag gggggtcatt
      721 gacgtcaacc acctccagga cacacgcgcc gctatcaccc tctcggcggg gacgctcgaa
      781 agggtcctgg gcgttcagcc tcctgccgag ggcgaggtcg agaggatctt cacggccctc
      841 gggtgcgaag tgcgtcggat cgagggcggg tggtcggtct cggcgccccc gttcaggccg
      901 gatgtggaga gggagatcga cctcgtggag gagttcgtcc gcatccacgg catggacaag
      961 gtcacgcccg agctccctgc cttcaggccg acgccgggtg gtgtatcccg cccgtcgctc
     1021 agggacctgc gcaggtccct ggccgccatg ggcttcaacg aggtcatcac ctactccttc
     1081 atctcgccca ggtggaagga cttcttcccc ggggattccc tgcggctcgt aaaccccatc
     1141 tccgacgaga tgagcctcat gcgcacctcg ctcgtccccg gcctgctggg cgcgctcgag
     1201 cgcaaccggc gcctgcagac gagggacgtc tgcatcttcg agatcgggag gaccttccac
     1261 cccgtcgagg gcgcggggct gcccagggag gaggagcggc tcggcgtgct catgtccggc
     1321 atgcgtttcg accagcactg gtcggatcag ccaaggcagg tggacttcta cgacctcaag
     1381 ggactggccg agctcctcat ccccggggtc acggtgaggc cgtctgacct gccgcacctc
     1441 gagagcgggc gccaggggga tctcgtcctc gacggcgagg tgatagggtg cctgggcgcg
     1501 gtgcacccgt ccatccgcac gatcatagac gcaagcgaag acgtgttcgt cctcgagacg
     1561 gtcctcgggc ccgtgatcga gaggcgatgg aaggggctgg ccgagatccc gcgcttcccc
     1621 ttcacgtggc gggacctctc gctcgtggtc gacgagaaga tcccctatgc cgacatcgtg
     1681 cgcgaggtgg aatccctggg cataggcgag atcaggaagg tcgcggcgct cgacgtatac
     1741 tcgggcgaga agctgccccg ggggaagagg ggcgtcacca tacgcatcac ctaccagtct
     1801 ctcgagcgca ccctcgagga cgccgagatc tcggcgtggc aggagcgggt gatctcgagg
     1861 ctcggcgaga gcctgggcat tacgctccga ggctagtttg gggaccgtcg cgcaagctcg
     1921 cccttgctgc gcgagcgggg gtggggcaaa gagggcgccc ggctgctgaa gcgggggggg
     1981 ggcccgcgtg cgcctgcaga cggcgtcttt cccgcgagcg gccggaaacg acaacctggc
     2041 cctcctgcaa gatcatccgc atgcgtcttc cccatgagcg gccggaaacg acacctgcct
     2101 gattgtgggg ccctcctgcc gggtcttacg tgcggggctg aacctgccgg ccgctcggga
     2161 atcgcaccgc atcgcatggc cgcccgccag gggcgggggc gcatcagcct atggcgatcg
     2221 cctccgtgac gatgcgctct gccgccctga gggtctcctt gaagagccgc gaggtcttcg
     2281 ttcccctggc cgccttcatg tccatgacct gggtgatgcc gagcatcatg ccccagatga
     2341 tgtccgacag catgtacggg ctcatctccc tgatcagccc gcgctccatc gccttggcga
     2401 gcagacgcct cgcgagctcg aagtcgtacc ttcccctgct gctgatggcg agccgcgtgc
     2461 tctcctccag ctcgccgatg aggtcggtgt tctggaagaa ggtgttggcc accctgaggg
     2521 agtcggggca tgcctcgaag ctcttctcga aggccctgaa caggtctttc acgagtttcg
     2581 acccgctcgc gtaccgaccc cgctccagcc tcctcgagat cgtctcgaag tgcgcaccga
     2641 actcctcgag gaagggcagc atgagggccg agtagagctc gtccttggtc ttgaagtact
     2701 ggtagatggt gggcttgctc acctgggcct cgagcgcgat ctcgtccatg gtggtcttca
     2761 ggtagccctt ggagaagaac accttcttgg cggcctggag gatcccctgt atccgcgcgt
     2821 tgcgttccct ctccctcctg gcctgtatcg ccctgcgatg gctctcggtc atgaggtctc
     2881 cgttccccgt cgtggttttt accgacagta gttcgattac tgccggtaag atgatgaaag
     2941 cactctctgc gaccgcgtgt caagccatga aatagggctt gcaggggctg cgccggggat
     3001 aggcgcctga gcctggacgg aaagacaggc ttgacagggc tggaaggatg caatataagc
     3061 ctactgttag taggaaagat accgatagtt tatgggcacg cgcactgtcc ggttcgccac
     3121 ccttgtcgtg ccctgggagg ttgcatggac ttcgaccttg ccccggagca gaggatgttc
     3181 agggagacgg tctaccgctg ggcgaaagaa gaggtggcgc ccttgtgcga ggatgcggac
     3241 ctgaggggcg cattcagccc cgagatctgg ggcaggctcg cggggttcgg catcctgggc
     3301 ctccctttcc ccgaggagtt cggcgggtcg ggggcggaca tcgtcacgtg ctgcctggcg
     3361 ggcgaggcgc tcggccacgc gggggtggac ggcggccacc tgctcgcctg gggcgcccac
     3421 acctacctgt gcgggcacaa catcctctct ttcggcaccg acgagcagaa gaggaagtac
     3481 ctgccgcgcc ttgcaagcgg ggagtggatc gggtgcatgg ggctgacgga gccgggctcg
     3541 ggctccgacg ccgcctcgct ctccacctcc gcgaggagga cgggctcggg ctacgtactc
     3601 aacggcacca agaccttcat caccaacgcc cccgtgagcg acgtgttcgt gatcttcgcg
     3661 aacgtagaca agaagctcaa gcacgagggg atcaccgcct tcatcgtgga gaaggggacc
     3721 ccgggcctgt ccaccggcag gccgttccac aagatgggct gcaggtgctc ggccacctcc
     3781 gaggtcatcc ttgaggactg cgaggtcccg gcggagaacg tcctcggcgg cgagggcatg
     3841 gggtggatca tcgcgctttc cggcgtcgag tgggaccggt gcacgctcct ctcccccatg
     3901 ctcggctgcg ccatgttcaa cctcgatgcg tgcgcgcgct acgcgaacga gcgccgccag
     3961 ttcggcaggc ccatcaggag tttccaggcg ataagcaggc gcatcgcgga catgaaggtc
     4021 ttcgtggagg cgctgaggct ggccatatac cgcgtggcgt gggccaagga caaggggagg
     4081 atgctcaatc ccctccaggc cgcggtgagc aaggtgtatg cgggcgacag gggcttcgag
     4141 atggcctccg aggcggtcca ggtcttcggc ggctacggct tcatgcacga gtaccccgtg
     4201 gagcgcaact tccgcgactc gaagctcgcg gccatcggcg gaggaacctc cgacatcatg
     4261 cgcatgatcg tctcgaggat catgctcatg gggttcgggg tgtgaggggg taagggcatg
     4321 gattacgaac tcgaaccgtc aagggtggat ttcctcaagg cgttgggcgc gcacctggcg
     4381 gagagggtgg cgcccaggcg cgcccagctg gactcgtgcg ggccggaaga acgcgcccgc
     4441 ctcatgcggg acaacatcgc cggcctcggg acgcacgggt ttctcgccgc catgtccatc
     4501 ggtaaccccc cggatctcgt gggccactac ctcgccctgg aagaaacggc gaaggcctgc
     4561 ccggccacct gcctgagcgc gcgcgcgagc gcgtttctct gcgcgggcgc gatctccctg
     4621 ttcggttcgc cgggcgcaaa ggagacatac ctgccccggc tcctgtcggg cgaggcggtg
     4681 ggcgcacttg cgtactccga gcccggttcg ggctccgacc tgggcgccct cggtgccgtg
     4741 gcggaaagcg acgcgggcgt ctacctggtc gacggagaga aggacatcgt ggtgaacgcc
     4801 ccgatcgccg aggtgttcgt cgtcctcgcg cgcacgcccg aagggccgtc cctgctcgtc
     4861 gtcgagggtg gggcccgagg tctcgagatc gggccgtgtc tcgacaccct ggggatcagg
     4921 ggggctcccg tgggcggcgt gaggatgcga cggtgcgagg cggcctgctt gctgggcagg
     4981 gagccgggca tgggcctcga acagctcgag aggctcctct ccatgggggc ggtcggcgcc
     5041 gcggccctgt gcgtgggaat agcacacgcc tgcatggaga tatcgaccga gagggccaag
     5101 agcaggtccg ccttcggcaa gcccataggc atgttccagg atgtcgggtt caagctcgcg
     5161 gacatgttca cgctcgtcga cctctcgcgc ctgctcgccc tgcgggcggc atgggccttc
     5221 gacacgggcg aaagagacgc cccggtacgg gcggcctgcg cgaagctctt ctcggccgag
     5281 gcgtgctcga aggtcgcgaa ctgggggatg cagatcttcg ccggccacgg ctacctggcg
     5341 ggaagcccca tcgagcgcct ctacagggac gcgagattcg gagagatctg cgagcggaca
     5401 tccgagatcc tccgcttgac gatagcgaac gaggagctcg ataggttcgc gggcttcgcc
     5461 tgatcgaggc ggcggccgcg cgataacgac gatgcaggga cgggacgagg catgcacgtg
     5521 atcgagacga agatcgatac cggatccgag gagtaccgga agaactacga ggccatggag
     5581 cggctcgtcg ccgacctgaa ggacgagctc cacaaggcga ggttcgagag gcccgccaag
     5641 gacatcgagc gggtgaagtc ccagggcaag atgctcgtca gggacaagct ggatctgctc
     5701 ctcgacaggg acagcccctt tctcgagatc gcgccgctcg cggcgagggg cctctacgac
     5761 ggaaaggtcc acc
//
LOCUS       DAOSCR010000090         5708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_8948_length_5708_cov_3.428622, whole genome shotgun sequence.
ACCESSION   DAOSCR010000090 DAOSCR010000000
VERSION     DAOSCR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8948_length_5708_cov_3.428622"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..278
                     /locus_tag="PLT69_03575"
     CDS             87..278
                     /locus_tag="PLT69_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOJ13554.1"
                     /translation="MGKTVFLDREECIGCGMCQDICPEVFELDEEGKARVIKPQGGPE
                     DLVEQAIDACPVSCIAFKE"
     gene            349..765
                     /locus_tag="PLT69_03580"
     CDS             349..765
                     /locus_tag="PLT69_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ13555.1"
                     /translation="MKTYRKEIWFNTKGRVEFVNITPEVEKCVRDSGVTEGLVLVNAM
                     HITASVFINDDEPGLHHDFAKWLDALAPYGPVDRYRHNVGEDNADAHLKRQVMGREVV
                     VAITGGRLDFGPWEQIFYGEFDGRRPKRVLVKIIGE"
     gene            complement(844..2220)
                     /locus_tag="PLT69_03585"
     CDS             complement(844..2220)
                     /locus_tag="PLT69_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ13556.1"
                     /translation="MAKVLVIDDDSSIRETLEMYLSEEGYEVYTAPTGTDGLNTFARV
                     TPDVVFLDIRLPDVNGFLILEDLKEEDENVKVIMITAHHDMESTIKAMKEGAFDYIHK
                     PIDVNELDIAVKKALHALEMEKKIKGFVESTRDYKVGDIIGRGPRMREIFKTIGVVSQ
                     SRTTVLIQGESGTGKELIARVIHNNTSPGEPYIAVNCSAIVETLLESELFGHEKGSFT
                     GAVTRKPGKFELARGGTVFLDEISEMSLNLQAKLLRVLQELEFERVGGKDRIRVNARI
                     IAATNKDLKAMVREGTFRNDLYYRLNIVTITIPPLRERMEDLEPLIEYLLSKINAELH
                     KRIAGISDEVLEMFRNYSWPGNVRELENLLVRAAVIAKGQVLVKSDFPDLADIQEKRE
                     TAGEDLGPFRNAQGRILTLDEVEELHIRRVLKDSKKTKGEICEILGISRPTFERKLER
                     YNIPFERD"
     gene            complement(2281..4857)
                     /locus_tag="PLT69_03590"
     CDS             complement(2281..4857)
                     /locus_tag="PLT69_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13557.1"
                     /translation="METRGSGPRRFPVRARYTFWITVPALAVLIVLLAITVYSVHEKA
                     VIELFGRQQATAARLYAGRIQDATAACGHALTLYAQAATHTGKAPEGLQAVHDELTPT
                     VTALALLGPDSAVLSAYPAQKGFFEKGSACDIPAMVNALERMHRRYVGEAWVVAGDGA
                     PGSEGCVSRSKVIVMGVPLLSKDGAYQGALVAAIHPKSLLGSQGGIPAPAGGAALLLD
                     ESGTVLFDPDPSHVGKAFDALLDTRSKRFRAFAYTHGGVLDVTMKGRDGPVAATAAFA
                     PVRLGSDRWWVVLVTPNDTVTGPVRRASLNILVGACGLVVVVMVTAFSVARAETKRLR
                     LSEELKRAREREEWQGRLLREKMTIEGIVQGSPVPMFVIDKDHKVILWNKACTELTGY
                     SAEEMIGTDNYYKPFYEKKRPFLADLIIDRNVDNIGSLYGEGGAVKSETIEGAYEAVR
                     HFPRLKGKDRHLYFLAAPIHDERGEIVAAIETFLDVTKEVELTRNLQEYNETLENELQ
                     ENIQLKQEVEQLYRYLQSILDSLPDKVYELNKDGIINYVSRQITTGMFDPKGRHFTEY
                     VAPEHREYAISKWEDAKRGIFKPYELELTGRDGKKRQLLMTPGPVRGTDRFVLVERDI
                     TELKELEKKYYESQKLAAIGQLSAGIAHEIRNPLSSIKMSLQILEKRMQPQGNDLKRF
                     KIAQREVEHLEKLVSDVLLYAKPMEPSKSPCDLRQVADQAIAMVEKSIEEKRIEVHRD
                     YPPDLHLVPVDPAMIGQALINVLQNAIDAMDVGGRLGVVLREEPKSASITVEDNGSGI
                     DEGDMPYLFNPFFTKKSYGTGLGLTQVEKIVTLHGGSIDVKSRKGEGTRFRIVLPRNA
                     AW"
     gene            complement(4912..5082)
                     /locus_tag="PLT69_03595"
     CDS             complement(4912..5082)
                     /locus_tag="PLT69_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13558.1"
                     /translation="MREIGAIAAYLISWLFCELVLGIESGLITLIVSLLFAFGIYALI
                     AFAEHEEGGRKA"
BASE COUNT          945 a         1731 c         1837 g         1195 t
ORIGIN      
        1 cgggggggcg tggcggcgcc tttgctgtac ttgcctttct tgcccggtgc ggctaccatg
       61 tgcgtacgct tcgaccggga gggcccatgg gaaagaccgt attcctcgac agggaagagt
      121 gtatcgggtg cgggatgtgc caggacatct gtccggaggt cttcgaactg gacgaggagg
      181 gcaaggccag ggtcatcaag ccccagggag ggcctgagga cctcgtcgaa caggccatcg
      241 acgcctgtcc ggtctcctgc atcgcattca aggaatgacg acggctcgtc gagccccggt
      301 caccatcccc ccgatggtgc atgccacata acggagcgcg ggaatgccat gaagacctat
      361 cgcaaggaga tctggttcaa cacgaaagga cgcgtcgagt tcgtcaacat cacccccgag
      421 gtcgagaaat gcgtgcgtga cagcggcgtc accgaggggc tcgtgctggt gaacgccatg
      481 cacataacgg ccagcgtgtt catcaacgac gacgaacccg ggctccacca cgacttcgcg
      541 aagtggctcg acgccctcgc gccttacgga cccgtggacc gataccggca caacgtcggg
      601 gaagacaacg ccgacgccca cctcaagcgc caggtcatgg ggagggaggt ggtggtggcg
      661 atcaccggcg gcaggctcga tttcggcccc tgggaacaga tcttctacgg cgagttcgac
      721 ggcaggcgtc ccaagagggt gctcgtgaag atcatcggag agtgagggcc gaggcgggca
      781 cgacgcggac ccaggcaggc cgccgtcacg gccgatgccc ttcgggcatg cgatccttcc
      841 gggtcaatcc ctctcgaagg ggatgttgta ccgctcgagc ttccgctcga aggtgggcct
      901 ggatatgccg agtatttcgc agatctcgcc cttggtcttc ttggagtcct tgaggacccg
      961 cctgatgtgg agttcctcga cctcgtcgag ggtgaggatc ctcccttggg cgttcctgaa
     1021 ggggcctaag tcctcgcccg cggtctctcg tttttcctgg atgtcggcga gatccgggaa
     1081 atcgctcttg acgagaacct ggcccttcgc gatgaccgcg gccctcacga ggaggttctc
     1141 gagctccctg acgttgcccg gccagctgta gttcctgaac atctcgagca cctcgtccga
     1201 tatccccgcg atgcgcttgt ggagctctgc gttgatcttg gagaggaggt actcgatgag
     1261 gggctcgagg tcttccatgc gttccctgag cgggggtatc gtgatggtca cgatgttgag
     1321 gcggtagtag aggtcgttcc tgaacgtccc ttccctgacc atggccttga ggtccttgtt
     1381 cgtggcggcg atgatcctcg cgttcaccct gatccggtcc ttgccgccga cgcgctcgaa
     1441 ctcgagctcc tggaggaccc tgaggagctt cgcctggagg ttgagggaca tctcgctgat
     1501 ctcgtccaag aacacggtgc cgccgcgtgc aagctcgaac ttgcctggtt tccgcgtcac
     1561 cgcgccggtg aaggacccct tctcgtggcc gaagagctcc gactcgagca gggtctccac
     1621 gatggccgag cagttcaccg cgatataggg ttcgccgggc gaggtgttgt tgtggatgac
     1681 gcgggcgatg agctccttgc cggtgccgct ctccccctgg atgaggacgg tggttcggct
     1741 ctgggacacc acgccgatgg tcttgaagat ctcccgcatc ctcgggcctc tgccgatgat
     1801 gtcaccgacc ttgtaatcgc gggtggactc gacgaacccc ttgatcttct tctccatctc
     1861 gagcgcgtgg agggccttct tcacggctat gtcgagctcg ttgacgtcta tgggcttgtg
     1921 gatgtaatcg aaggcccctt ccttcatggc cttgatggtg gattccatgt cgtggtgggc
     1981 ggtgatcatg atgaccttga cgttttcgtc ctcttccttc aggtcctcca ggatgagaaa
     2041 gccgttgacg tcgggcagcc tgatatcgag gaagaccaca tccggggtca ccctggcgaa
     2101 ggtattgagc ccgtcggtac cggtcggtgc cgtgtagacc tcgtaaccct cctcggagag
     2161 gtacatctcc agggtctcgc ggatggagct gtcgtcgtcg atgacgagga ctttcgccat
     2221 ggtgctggaa tcctccggaa cgcgtaaagg gtgcgcgcgt cgatctgatc agcgtttata
     2281 tcaccaggcg gcgttccggg gaaggacgat cctgaacctg gtgccttccc ccttgcggct
     2341 cttcacgtcg atggacccgc cgtgcagggt cacgatcttc tccacctggg tcagcccgag
     2401 gcctgtgccg tagctcttct tcgtgaagaa ggggttgaac aggtagggca tgtcgccctc
     2461 gtctatgcct gaaccgttgt cctccacggt tatcgatgcc gacttcggtt cttccctgag
     2521 cacgacaccg agtctcccgc ccacgtccat ggcgtctatc gcgttctgga gcacgttgat
     2581 gagagcctgg ccgatcatgg cggggtcgac cggcacgagg tggagatcag gggggtagtc
     2641 cctgtgcacc tcgatgcgtt tctcctcgat ggacttctcc accatcgcga tcgcctggtc
     2701 cgccacctgc ctcaggtcgc agggggattt cgacggctcc atgggtttgg cgtagaggag
     2761 cacgtcgctc acgagcttct ccaggtgttc cacctcccgt tgggcgatct tgaacctctt
     2821 gaggtcgttg ccctggggct gcatgcgttt ctccaggatc tgcaggctca tcttgatgga
     2881 ggagaggggg ttccggatct cgtgggcgat gcctgcggag agctggccga tggcggcgag
     2941 cttctggctc tcgtagtatt tcttctcgag ctccttgagc tcggtgatgt cgcgctcgac
     3001 gaggacgaag cggtccgttc ccctcacggg gcccggggtc atgaggagtt ggcgtttctt
     3061 cccgtccctg cccgtgagct cgagctcgta cggcttgaaa atgccccgct tcgcgtcctc
     3121 ccacttggag atcgcgtatt cgcggtgctc cggcgccacg tactcggtga agtgcctgcc
     3181 cttcgggtcg aacatgccgg tggtaatctg cctgctgaca tagtttatga taccgtcctt
     3241 gttgagctcg tagaccttgt cagggaggct gtccaggatg ctctggaggt aacggtagag
     3301 ctgctccacc tcctgcttga gctggatgtt ctcctggagc tcgttctcga gggtctcgtt
     3361 gtattcctgg aggttgcgcg tgagctccac ctccttggtc acgtcgagga aggtctcgat
     3421 ggccgcgacg atctcacccc gctcgtcgtg gatgggggcg gcgaggaagt agagatgccg
     3481 gtctttcccc ttgagcctcg ggaagtgcct caccgcctcg tacgccccct cgatggtctc
     3541 ggacttgacc gcaccgccct ctccgtagag cgatccgatg ttgtccacgt tccggtcgat
     3601 gatgaggtcg gcaaggaagg gccgtttctt ttcgtagaac ggcttgtagt agttgtccgt
     3661 gccgatcatc tcttcggcgg agtacccggt gagctccgtg catgccttgt tccagaggat
     3721 caccttgtgg tccttgtcga tgacgaacat gggcaccggg gaaccctgga cgatgccctc
     3781 gatggtcatc ttctccctga ggagcctgcc ctgccattct tcccgctccc tggccctctt
     3841 gagctcttcg gagagcctca gcctcttcgt ctccgccctg gccacggaga aggcggtgac
     3901 catgaccacc acgacgagcc cgcaggctcc caccaggatg ttgagcgaag cccttctcac
     3961 cgggcccgtg accgtatcgt tcggcgtgac gagaacgacc caccacctgt cgctcccgag
     4021 ccttacgggc gcgaaggcgg ccgtggcggc gaccgggccg tctcttccct tcatcgttac
     4081 gtcgagaacc ccgccgtggg tatacgcaaa ggccctgaac ctcttggatc gggtatccaa
     4141 cagcgcgtcg aacgccttgc ccacgtgcga ggggtcggga tcgaacagca cggttcccga
     4201 ctcgtccagg aggagggcgg cccctccggc aggcgcgggg atgccgcctt gcgaacccag
     4261 gagggacttg gggtggatcg ccgccaccag cgcaccctgg taggcgccgt ccttcgacag
     4321 gagggggaca cccatgacga tcaccttgga cctcgagacg catccttcgg aaccgggggc
     4381 gccgtctccc gcgaccaccc aggcctcgcc gacgtaccgg cggtgcatcc tctccagggc
     4441 gttcaccatg gcggggatgt cacaggcgga tcctttctcg aaaaagccct tctgggcggg
     4501 gtacgccgac agcacggcgc tgtccggtcc caggagggcc agggccgtga cagtgggtgt
     4561 gagctcgtcg tggacggctt ggagtccctc gggggccttt cctgtgtgag tcgcggcttg
     4621 ggcgtacagg gtcagcgcat gcccgcacgc cgcagtcgcg tcctgtatgc ggccggcata
     4681 gagccttgcc gccgtggcct gctgcctgcc gaagagctcg atgacggcct tctcgtgcac
     4741 cgagtacacg gtgatggcca gaaggacgat gagaaccgcg agtgcgggga cggtgatcca
     4801 gaaggtgtac ctggcccgga cggggaaccg tctcggtcct gaccctcttg tctccatggg
     4861 gtgatcgtgg gccggtgggc ttccgtgcat ccctccgtga gcctgcatcc tttacgcttt
     4921 cctgcccccc tcctcgtgct cggcaaaggc tatgagggcg tagatgccga aggcgaagag
     4981 gagcgagacg atgagggtga tgaggccgct ctctatgccc aacacaagct cgcagaacag
     5041 ccacgatatc aggtatgccg cgatagcgcc gatctctctc acgatgcttt tgcctgtttc
     5101 cggccgagcc tttgggccgt tggttgtggt atattgtata gggaaaggac gccgtgttca
     5161 atacccagtg ggtgaggggc gcccccgtcc ttgtccgtcc cgtggatagg cctgcgtgga
     5221 catgtctttg gggtcacggg cgtaccggga agaaggcccg tccacggggc cgtgcggcga
     5281 atgcccggtg accgcccgac ggcacctttc gttcaacggg gacacgaggc gttgaacgat
     5341 ccgtacaacg acacgagggc aacggcccgg gggaaaggcc gctgccgccc cgaaacaacc
     5401 aacgataacg gtgagatatg ggggacgagg acgatgagag ggtgcatggc atggctgttg
     5461 caaaacatgc tgcaaacatt gagtgctcac tcaatgtgga cgggaaaaat atcttcagag
     5521 ggaaaggaga ttgacatgaa tcggaaagcc acccaggttc tgttcacggt tgcagccacg
     5581 gctctcgtct ggatcctcgg atcgccggcg gtcttcgcgg ctgatgccat ccccgcgaac
     5641 gatcccctca tcaaggtgat tttctctgtg ggcgccatga tcggggcggg tctcgccatc
     5701 gggctcgg
//
LOCUS       DAOSCR010000091         5674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9002_length_5674_cov_3.466097, whole genome shotgun sequence.
ACCESSION   DAOSCR010000091 DAOSCR010000000
VERSION     DAOSCR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_5674_cov_3.466097"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLT69_03600"
     CDS             <1..645
                     /locus_tag="PLT69_03600"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOJ13559.1"
                     /translation="KAKQARRIIAADAGARVCLENGITPDLVVGDMDSLGEEVLGRLE
                     GAGVPLKTFDTDKDRTDTEIAFDEAVHAGASSIELLGALGMRTDHAIANIHLLLKAQR
                     RGVPSFIVTDTELVFVVDTETCIESEGGHVVSFLPLTDTVEGIVLDGFLYPLEGASME
                     IGRPYGVSNVLLEGRSLVSVGKGALLGLIVFSEGVRGVRVMTRRPDGAMVCADR"
     gene            698..1618
                     /locus_tag="PLT69_03605"
     CDS             698..1618
                     /locus_tag="PLT69_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ13560.1"
                     /translation="MKRIFFVLSLVAVAALVYSCSRQGSGPVDEKDVVARVGDKVITA
                     AQIDQFLENIPPQMAGKVNTDKIRREIAEGFVKAEIMAIEARRRGLDKREDVKLKINM
                     LASQALARELEKELRKEMKLSDEELRKYYDENKDRFSAKRRMRASRITVPTEAAAKDV
                     IARINKGEDFAELAKKFSKDVYAQRGGSMGTIRPGKLEPEIENALSSLQEGKVSKAVK
                     TGQGYVIVRLDSVVDIPERSFEEAKPRIERILVRERLEKALKEIDAEVRKKNRVVINE
                     GYFVKYGKPAPAAPQAAPGPEKAPGADAEE"
     gene            1645..2868
                     /gene="gspF"
                     /locus_tag="PLT69_03610"
     CDS             1645..2868
                     /gene="gspF"
                     /locus_tag="PLT69_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOJ13561.1"
                     /translation="MAVYSWTGYTQKGKTASGMIDAASVREAKLKLMSQGMFVSSIAE
                     ASREVRPSSRAFSIEALLGRVSTEDLAVATRQLSTLVGAAIPLVDALTALFEQTESPT
                     MKRTIAQVKDSVNEGVSFADALSRHRRIFPELYVNMVRSGEASGALDVVLLRLAEFLE
                     GQHRLKSRVQAALVYPAVLLLVSIVVLMYLLTAVVPKVVGMFETMNQVLPLPTRVLIG
                     LSAFVSRTWWILLVAVALSLYALKRWRATPRGAELFDRFRMRVPIYGGVFRKVSVARF
                     ARTLGTLLASGVPIVEALRIVKAVVVNKVMEIAIEDTITEVIEGSSLSAPLRRSGVFP
                     PLLVHMIGVGEKSGNLEEMLVKAAQSYESDVETTVSGLASVLEPVMILFMGGVVGFVV
                     MSILMPMLEMSTIVR"
     gene            2886..3350
                     /gene="gspG"
                     /locus_tag="PLT69_03615"
     CDS             2886..3350
                     /gene="gspG"
                     /locus_tag="PLT69_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOJ13562.1"
                     /translation="MERMDRKGFTLIELLVVIVILSILATFVGIRIMGKPEEARRTQA
                     QIQIQALENALKMYRLDNGAYPSTEQGLRALVEKPTVGTVPRNWREGGYLEKRQVPKD
                     PWKNEYMYLCPGVRNPDGFDLFSYGADGQPGGEGDDADIGNWETEDETGGQQ"
     gene            3298..3846
                     /locus_tag="PLT69_03620"
     CDS             3298..3846
                     /locus_tag="PLT69_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ13563.1"
                     /translation="MRTSGTGRPKTKPAASSRAQVGFTLIELLVVIVIIGITVGFVGP
                     RIFGGIFASTMDRGTRDIANVIQLARSRAVKEHKTYFVRFDLDAEKVAVYRMVEEEGK
                     EPALSSEISLPDGVDLREVKTPYQPEKDRGFLDLKVTPEGIVEQGVIYLEGPLGRVYT
                     LVVKPFSGALKVHDHYVEVILG"
     gene            3839..4204
                     /gene="gspI"
                     /locus_tag="PLT69_03625"
     CDS             3839..4204
                     /gene="gspI"
                     /locus_tag="PLT69_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOJ13564.1"
                     /translation="MARRGFTLIEVLVALTILAIAFTWLISAHGQSIDMATRSRFLTT
                     ATLLAQERIAAVTTGLVPVPETGRSGDFGEDHPGYTYEEDVESTPIPGYLKYTLKVKW
                     GAQERPLETRFVAFVSRTQ"
     gene            4164..4859
                     /locus_tag="PLT69_03630"
     CDS             4164..4859
                     /locus_tag="PLT69_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ13565.1"
                     /translation="MRRDSSRSCPGPSSGFTLIELLLAFVITAVVVATVNFVFFETHK
                     TIEAVVERREVYQTARIVLDRMVRDLNCAYVPSGGGAVSDDALSMWRFVGEHEEEGAV
                     SRDSLTFTTAADIGFSRYPGCVREVAYYLEEDAENKGTYRLLRREDPTPHKGASTAGA
                     VLEMAEGVGGLKIVYVDEYGQENDSWDLADRRKLPRSIRVTVRLGSDEQAVDFTATAS
                     PPLAGITVSSSGG"
     gene            4864..>5674
                     /gene="gspK"
                     /locus_tag="PLT69_03635"
     CDS             4864..>5674
                     /gene="gspK"
                     /locus_tag="PLT69_03635"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HOJ13566.1"
                     /translation="MRWILAGRSGMALLLVLGMVALLSVMVVGFSSEEGIDIELAYNF
                     RDSVQAQQIARGVVEAAVAVLEEDDPSHDSKDEQWGGFSQYVAGASTYLEGATVDASI
                     QDESGKIDLNALAANDGYREMRIAQFQRLFSLLHVDATEEEVREIAYAVIDWIDEDGE
                     TELGAEDDYYQSLDPPYHCKNAPMDTPEEILLVKGMKPEYFFGTKDHEGIVNYVTTGT
                     NGRINVNTASETVLMSLSESAGEQVVANIVDCRPFLSNDLGCISCLDLSGQG"
BASE COUNT         1119 a         1643 c         1985 g          927 t
ORIGIN      
        1 aaggcaaagc aggcaaggcg tatcatagca gctgatgcag gcgcccgcgt gtgcctggag
       61 aacggcatca cccccgacct cgtcgtgggc gacatggatt ccctggggga ggaggtcctc
      121 gggcggctgg aaggggcggg cgtacccctg aagacgttcg acacggacaa ggacaggacg
      181 gacaccgaga tcgccttcga cgaggcggtc catgcagggg cctcgagcat agagctgctg
      241 ggcgcgctgg gaatgcgcac cgatcacgcc atagccaaca tccacctcct cctcaaggcg
      301 cagaggaggg gcgttccctc tttcatcgtc accgacacgg aactcgtgtt cgtggtcgac
      361 acggagacgt gcatcgagtc tgagggcggg cacgtggtgt ccttcctccc gctgacggac
      421 acggtggaag gcatcgtgct cgacgggttt ctgtaccccc tcgagggcgc atccatggag
      481 atcgggaggc cctacggggt gagcaacgtg ctgctcgagg gacgatcgct ggtctcggtc
      541 ggcaagggcg cccttttggg tttgatcgtc ttcagcgaag gtgtgagggg agttcgggtg
      601 atgacacgga gacccgatgg tgctatggtg tgcgccgatc gatgaagtga cccttcgatc
      661 acacggaaac gagggaaaac atatcaagga gggagccttg aagaggatct ttttcgtact
      721 gtcgctggtc gctgtggctg cgctcgtgta ttcgtgctcc aggcaggggt ccgggcctgt
      781 cgacgagaag gacgtggtgg cgagggtggg tgacaaggtc ataaccgcag cacagatcga
      841 ccagtttctc gaaaacatcc cgccccagat ggcggggaag gtgaacaccg acaagatccg
      901 cagggagatc gccgagggct tcgtgaaggc ggagatcatg gccatcgagg cacgcaggcg
      961 gggtctcgac aagcgggaag acgtcaagct caagatcaac atgctggcga gccaggcgct
     1021 cgcccgggag ctcgagaagg aattgcgcaa ggaaatgaag ctgtccgacg aggaactgag
     1081 gaagtattac gatgaaaaca aggaccgctt ctccgcgaag aggcgcatga gggcgagcag
     1141 gattacggtg cccacagagg ccgcggcgaa ggacgtcatc gccaggatca acaagggtga
     1201 ggactttgcg gagctcgcga agaaattctc caaggacgtc tacgcccaaa ggggcgggtc
     1261 gatgggcacc atcaggcccg gcaagctcga gcccgagata gagaacgccc tttcgtcgct
     1321 ccaggagggc aaggtgagca aggccgtgaa gaccggccag ggatacgtga tcgtccgtct
     1381 cgacagcgtc gtggacatac cggagcggag cttcgaggag gcaaagccca ggatcgagag
     1441 gatactcgtc cgcgaaaggc tggagaaggc gctcaaggag atcgacgccg aggtcaggaa
     1501 gaagaacagg gtcgtgatca acgagggata tttcgtgaag tacggcaaac cagcgccggc
     1561 cgccccgcag gccgcgcccg ggcccgagaa ggcgccgggg gcggacgctg aggagtaggc
     1621 gggtcggttc ccgggggagc ggccttggcg gtctacagct ggacagggta tacccagaag
     1681 ggtaagacgg cgtccggcat gatcgacgct gcctccgtca gggaggccaa gctcaagctc
     1741 atgtcccagg gcatgttcgt gagctccatc gccgaggcct cgcgcgaggt gaggccctcg
     1801 agcagggcgt tctcgatcga ggccctgctc gggcgtgtga gcacggagga ccttgccgtg
     1861 gccacccggc agctctccac gctcgtgggc gccgcgatcc ccctggtcga cgccctgacc
     1921 gcgctcttcg agcagaccga gtcgccgacc atgaagagga cgatagccca ggtcaaggac
     1981 tcggtgaacg agggggtgtc cttcgccgac gccctctccc gccacaggcg catcttcccg
     2041 gagctctacg tgaacatggt gcgctcgggg gaggcgagcg gagccctgga cgtcgtgctc
     2101 ctgaggctcg ccgagttcct ggagggccag caccggctga agtcgagggt gcaggcggcg
     2161 ctcgtctacc ccgcagtcct gctcctggtg tccatcgtgg tgctcatgta cctcctgacc
     2221 gcggtcgtcc ccaaggtcgt ggggatgttc gagaccatga accaggtcct tccgctgccc
     2281 acgagggttc tcatcggcct gagcgccttt gtgtcccgga cgtggtggat cctgctcgtg
     2341 gccgtggccc tctcgctgta cgccctgaaa cgctggaggg ccacgccgag gggcgcggaa
     2401 ctcttcgaca ggttcaggat gcgggtcccc atctacgggg gcgtgttcag gaaggtctcg
     2461 gtggcgaggt tcgcgcgcac gctggggacc ctgctcgcct ccggcgtgcc catcgtggag
     2521 gcgctcagga tcgtgaaggc ggtcgtggtg aacaaggtca tggagatcgc gatcgaggac
     2581 accatcacgg aggtcatcga gggctcgagc ctgtccgcgc cactgaggcg ctcgggcgtc
     2641 ttcccgccct tgctcgtcca catgatcggc gtgggggaga agagcgggaa tctcgaggag
     2701 atgctcgtga aggcggccca gtcctacgag tccgacgtgg agacgacggt gtcgggcctc
     2761 gcgtcggtgc tcgagcccgt catgatcctc ttcatggggg gcgtggtggg gttcgtggtg
     2821 atgtcgatcc tcatgcccat gctcgagatg agcacaatag tacgctagca caagaggttg
     2881 accctatgga acgaatggac aggaaaggat tcaccctcat cgagctgctc gtggtcatag
     2941 tcatcctgag catcctcgcg acgttcgtgg ggatcaggat catgggcaag cccgaggagg
     3001 cgaggagaac gcaggcccag atccagatcc aggcgctgga gaacgccctc aagatgtaca
     3061 ggctcgacaa cggggcgtac ccctcgacgg agcagggtct cagggcgctc gtggagaagc
     3121 ccacggtcgg gaccgtgccg aggaactggc gggaaggtgg atacctggag aagagacagg
     3181 tgccgaagga cccctggaag aacgagtaca tgtacctgtg cccgggggtt aggaaccccg
     3241 acgggttcga cctcttctcc tacggcgccg acggccagcc cggcggagaa ggcgacgatg
     3301 cggacatcgg gaactgggag accgaagacg aaaccggcgg ccagcagtag agcccaggtc
     3361 ggcttcaccc tcatagagct ccttgtggtg atcgtcatca tcgggatcac ggtgggattc
     3421 gtggggccca ggatcttcgg cgggatcttc gcctccacca tggaccgggg cacccgggac
     3481 atagccaacg tgatccagct cgcgagatcc agggcggtca aggagcacaa gacctacttc
     3541 gtcaggttcg acctggacgc cgagaaggtg gccgtctatc gcatggtgga agaggagggc
     3601 aaggaacccg ccctcagctc ggagatatcg cttcccgacg gcgtggacct gagggaggtg
     3661 aagacgcctt accagccgga aaaggacagg gggttcctcg acctcaaggt gacgccggag
     3721 ggcatcgtgg aacagggggt gatatacctc gaggggcccc tggggagggt ctataccctc
     3781 gtcgtcaagc ccttctcggg cgccctgaag gtccatgacc actacgtcga ggtgatcctt
     3841 ggctagaagg gggttcaccc tcatcgaggt cctcgtcgcc ctcaccatcc tcgccatagc
     3901 ctttacgtgg ctgatcagcg cgcacggcca gtccatagac atggccacgc gctcgaggtt
     3961 cctcaccacg gcgactctct tggcacagga gcgcatcgcc gcggtcacca cgggactggt
     4021 cccggttccc gagacaggcc gcagcggcga cttcggagag gaccaccccg ggtacaccta
     4081 cgaggaagac gtggaatcca ctcccatccc cggctacctg aagtataccc tgaaggtgaa
     4141 gtggggcgca caggagagac cccttgagac gagattcgtc gcgttcgtgt ccaggaccca
     4201 gtagcgggtt caccctcatc gagctcctgc tggcgttcgt gatcaccgcg gtggtggtcg
     4261 cgaccgtcaa cttcgtgttc ttcgagaccc acaagaccat cgaggccgtg gtcgaaaggc
     4321 gcgaggtcta ccagacggca aggatcgtgc tggaccgcat ggtgagggac ctgaactgcg
     4381 cttacgtgcc gtcgggaggg ggcgccgtga gcgacgacgc cttgtccatg tggcggttcg
     4441 tgggcgagca cgaagaggag ggggccgtct cgagggacag cctcaccttc accacggcgg
     4501 cggacatcgg gttttcccgg tatcccgggt gcgtacggga ggtggcctac tatctcgagg
     4561 aggacgcgga aaacaagggt acatacaggc tgttgaggag ggaggacccg acgccccaca
     4621 agggggcgtc gaccgccggc gccgtgctgg agatggcgga aggggtgggc gggctcaaga
     4681 tcgtctacgt ggacgagtac gggcaggaga acgactcgtg ggacctcgcc gaccggcgga
     4741 agctgcccag gagcatcagg gtgaccgtcc gcctcggttc cgacgagcaa gccgtcgact
     4801 tcacggcgac cgcctcgccg ccgctggcgg ggatcaccgt ctcgtcgtcc ggggggtgac
     4861 gcgatgagat ggatcctggc cgggcgatcc ggcatggcgc tccttctggt gctgggcatg
     4921 gtggccctct tgtccgtcat ggtcgtgggg ttcagctcgg aggagggcat tgacatcgag
     4981 ctggcataca acttccgcga ctcggtccag gcccagcaga tagcccgggg cgtggtggag
     5041 gccgccgtcg cggtcctgga ggaggacgac ccttcccacg actcgaaaga cgagcagtgg
     5101 ggagggttct cgcagtacgt ggcgggagct tccacgtatc tcgaaggagc gaccgtcgat
     5161 gcctccatac aggacgaatc cggcaagatc gacctcaacg cgttggcggc gaacgacggc
     5221 tatcgagaga tgcggatagc gcagttccag aggctcttct ccctgctcca cgtggatgcg
     5281 accgaagagg aggtccgtga gatcgcctac gcggtgatcg actggatcga cgaggacggc
     5341 gaaacggagc tgggcgctga ggacgactac taccagtcgc tcgatccgcc ctaccactgc
     5401 aagaacgcgc ccatggacac ccccgaggag atcctgctgg taaagggcat gaagcccgag
     5461 tacttcttcg gcacgaagga ccacgagggg atcgtgaact acgtgacgac agggacgaac
     5521 ggcaggatca acgtcaacac cgcgagcgag acggtcctga tgagcctgtc ggagtcggcg
     5581 ggggagcagg tcgtggcgaa catagtcgac tgcaggcctt tcttgagcaa cgacctgggg
     5641 tgcatttcct gtctcgacct gtcgggccaa ggcg
//
LOCUS       DAOSCR010000092         5660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9028_length_5660_cov_3.756111, whole genome shotgun sequence.
ACCESSION   DAOSCR010000092 DAOSCR010000000
VERSION     DAOSCR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9028_length_5660_cov_3.756111"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /gene="uvrB"
                     /locus_tag="PLT69_03640"
     CDS             complement(<1..1567)
                     /gene="uvrB"
                     /locus_tag="PLT69_03640"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOJ13567.1"
                     /translation="MAYRLVSPFAPKGDQPRAIEALCEGIEGGRRDQVLLGVTGSGKT
                     FTMACVIAQTGRPALVIAPNKILAAQLYGEFKELFPDNAVEYFVSYYDYYQPEAYVPS
                     KDLYIEKDSSINEDIDRMRHRATRALFEQQECIIVASVSCIYGLGSPEAYQGMLLYLE
                     EGRVMPREDILKKLVEIQYTRNDYDFHRGTFRVRGDTIEVFPAHEEDTAVRIELFGDV
                     IDAIWQMDAIRAKKKARLNHVPIYPASHYVTPRSRLIDAIGSIREELAQRIVFFRSEG
                     RLVEAQRIEERTRYDLELLLETGTCKGIENYSRHLTGRRPGEPPPTLVDYLPKNTIVF
                     LDESHVSVPQLIGMYRGDHSRKSTLVEYGFRLPSALDNRPLRFDEFNRLVHRIIYVSA
                     TPGDYELKQAGGRVVEQVVRPTGLLDPKIEVKPAQGQVDDLYREIVQAVRKGGRVLVT
                     TLTKRMAEELTDYLQGLDLKVKYMHSDIDTIERIEIVKGLREGEFDVLVGINLLREGL
                     DLPEVTLVAVLDAD"
     gene            complement(1680..3116)
                     /gene="cysS"
                     /locus_tag="PLT69_03645"
     CDS             complement(1680..3116)
                     /gene="cysS"
                     /locus_tag="PLT69_03645"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOJ13568.1"
                     /translation="MQLTIYNTPLKKKKPFEPLAGKKVGMYVCGVTVYDMCHVGHARS
                     LLVFDVIARYLRRCGYDLTYVRNFTDIDDKIIARANTLGIDYREVADRYIEEFATDMK
                     SLGVLNADIEPKATDHIPQIIDMVRILQDKGYAYPAPDGSVYFSVAKFRTYGGLSGRR
                     TEEMIAGARVDIDEMKRDPLDFALWKRSKEGEPWWESPWSRGRPGWHIECSAMSTHYL
                     GPTLDIHGGGRDLVFPHHENETAQSEAAFGVPFVRYWVHNGFVTVAGEKMSKSLGNFM
                     TIRDLLKDVHPEVLRLLVLSHHYRSPIDFSKDAISVAKGALVRFYEMLHRVSTSTSTG
                     DASRIERFVSFFTEEVGEAMADDFNTARCIAALHELTTEVNRLLDNDPRISKADRAAL
                     DTAVAEVRDLFGILREEPAVFLDAVKRSGVSLSGLSEEEIERLVEERNQARKAKDFRR
                     ADEIRDMLKAKGIVLKDTPGKTIWEKSM"
     gene            complement(3248..3727)
                     /gene="ispF"
                     /locus_tag="PLT69_03650"
     CDS             complement(3248..3727)
                     /gene="ispF"
                     /locus_tag="PLT69_03650"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOJ13569.1"
                     /translation="MPCRIGIGYDIHRLVAGRPLVIGGVKIPHDRGLLGHSDADVVVH
                     AVCDAILGALAAGDIGEHFPDTSPETEGMDSLEMLRRVAAMLGGRLRIANIDINCICE
                     RPRLSGHRREMVTAISTAAGVDPSAVSIKFRTSEGLGPVGLGEAVSAQAVVLLEEVA"
     gene            complement(3706..4389)
                     /gene="ispD"
                     /locus_tag="PLT69_03655"
     CDS             complement(3706..4389)
                     /gene="ispD"
                     /locus_tag="PLT69_03655"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOJ13570.1"
                     /translation="MPPLDVVIVAGGTGLRYGARKQFLDLGGIPVLRRSAECFERCLP
                     CDRIVVAVPPEDVERASGILQGMAVELIVTPGGATRRESVYNALRHCRPGGTVVVHDG
                     VRPLVAPELARRVVSGLEGCHGCIPALPLTDTVKEAREGFVARTVPRAGLVCVQTPQA
                     FVTDALVEAHQKAHRHKKAAYTDDSSLLEEAGMRVRIVDGDPLNIKITYPADLALAEA
                     ILRCRAGSV"
     gene            complement(4376..5365)
                     /locus_tag="PLT69_03660"
     CDS             complement(4376..5365)
                     /locus_tag="PLT69_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOJ13571.1"
                     /translation="MKWLLRILIAVIVFYGAYQYALVQMKGGLWLVILPIIALSCLAA
                     VFLGEIRGFKLHPMAYAGGLVGTILGLIIGSLFGYVLLNINESGIYLFILVNTTFAYL
                     GYTIGLNWGKEISQLPIFRQRSKTEGARMKILDTSVIIDGRIADICEAGFIEGTIVAP
                     QFILNELQHIADSPDPLKRARGRRGLDILNKLRKSPGVKIEVMDIDFPKIREVDSKLI
                     ALAREIGADILTNDFNLNKIAQLQGVNVLNINQLANAVKPIVLPGETLTVNITRPGKE
                     RGQGVAYLDDGTMVVVEQGESLINQSIEVTVTSILQTPAGRMIFGQPKNHASA"
     gene            complement(5407..>5660)
                     /locus_tag="PLT69_03665"
     CDS             complement(5407..>5660)
                     /locus_tag="PLT69_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOJ13572.1"
                     /translation="NQTWNRRYREYMEKIKSGSAFEIAEVLRDLNILKKGKELSFGER
                     KMYDTAKNLLVNEISISGKMDKKEAEKMILTALGRTETP"
BASE COUNT          892 a         1803 c         1800 g         1165 t
ORIGIN      
        1 tgtcagcatc cagcaccgcc acgagcgtca cctcggggag gtcgagccct tccctgagga
       61 gattgatgcc cacgagcacg tcgaactccc cttccctcag ccccttgacg atctcgatgc
      121 gttcgatcgt gtctatgtca gagtgcatgt acttcacctt caggtccagt ccctggaggt
      181 agtcggtaag ctcctcggcc atgcgcttgg tcagcgtggt gacgagcacg cggccccctt
      241 tcctcaccgc ctggacgatc tcccggtaca ggtcgtccac ctggccttgg gcgggtttca
      301 cctcgatctt ggggtctaaa agcccggtcg gcctcaccac ctgctccacc acccggcctc
      361 ctgcctgctt gagctcgtaa tcgcccggcg ttgcggagac gtagatgatc ctgtggacga
      421 gcctgttgaa ctcgtcgaac cgaagaggcc tgttgtcgag tgcagaaggg agcctgaagc
      481 cgtactccac cagcgtggac ttccgcgagt ggtccccgcg gtacatgccg atgagctggg
      541 gtacggacac gtgggactcg tccaggaaga cgatcgtgtt cttggggagg tagtcgacga
      601 gggtgggcgg cggctcgccg ggtctgcgcc ccgtgaggtg cctcgagtag ttctcgatcc
      661 ccttgcaggt gcctgtctcc aggagcaact cgaggtcgta gcgtgtccgc tcctcgatcc
      721 gctgggcctc cacgagtctc ccttcggacc tgaagaagac tatcctctgg gcgagttcct
      781 ccctgatgga ccctatggcg tcgatgagcc tgctcctggg cgtgacgtaa tgggatgccg
      841 ggtagatggg cacgtggttc aggcgggcct tcttcttcgc gcggatggca tccatctgcc
      901 agatggcgtc gatgacgtcg ccgaacagct cgatcctcac ggccgtgtct tcctcgtgcg
      961 cagggaagac ctctatggtg tcgcccctga ccctgaatgt gcccctgtgg aagtcgtagt
     1021 cgttccgggt gtactggatc tccacgagtt tcttgagaat gtcctccctg ggcatcaccc
     1081 ggccttcctc gaggtagagg agcatgccct ggtaggcctc cggcgagccc aggccgtaga
     1141 tgcaggagac cgaggccacg atgatgcatt cctgctgttc gaacagggcg cgggtagccc
     1201 tgtggcgcat cctgtcgatg tcctcgttga tggacgagtc cttctcgatg tagaggtcct
     1261 ttgacggcac gtaggcctcg ggctggtagt agtcgtagta gctcacgaag tactcgacgg
     1321 cgttgtccgg gaagagctcc ttgaactccc cgtagagctg tgcggccagg atcttgttcg
     1381 gcgctatgac gagcgccggc cttcccgttt gggcgatgac gcacgccatg gtgaaggtct
     1441 tgccggagcc ggtgaccccg agcaggacct ggtcgcgcct gcccccctcg atgccttcgc
     1501 acagggcctc gatggccctg ggctggtcgc ccttgggtgc gaaaggtgac acaagcctgt
     1561 acgccatgtc aaaggatcac cgtgcgcgcc tcaggctgcc tttcggtggg gatgtccccc
     1621 tcgcggtgag gccgcaagcc cgacgggtgc cggcgtgtcc tcgacgcagg ccctctccgt
     1681 cacatcgact tttcccatat ggtctttccc ggggtgtcct tcaggacgat gccctttgcc
     1741 ttgagcatgt cccggatctc gtccgcacgc ctgaagtcct tggccttgcg cgcctggttc
     1801 cgttcctcga cgaggcgctc gatctcctcc tcggagagcc ccgaaaggga aacgcccgag
     1861 cgtttcaccg catcgaggaa gacggcgggc tcctccctga ggatgccgaa gaggtccctg
     1921 acctctgcga ccgccgtgtc gagggcagcc cggtcagcct tcgagatccg tgggtcgttg
     1981 tcgagcaggc ggttcacctc ggtggtgagc tcgtggaggg ctgcgatgca ccttgccgtg
     2041 ttgaagtcgt ctgccatggc ctcgcccacc tcctcggtaa agaaggagac aaaccgctcg
     2101 atgcgcgagg cgtccccggt cgatgtcgag gtcgagaccc tgtggagcat ctcgtagaag
     2161 cgcacgaggg cgcccttggc cacggagatg gcgtccttcg agaagtctat ggggctccgg
     2221 taatggtgcg agaggacgag cagcctcaag acctcgggat gcacgtcctt gaggaggtcg
     2281 cggatcgtca tgaagttccc gagggacttg ctcatcttct cgcccgccac ggtcacgaac
     2341 ccgttgtgca cccagtagcg cacgaacggc acaccgaagg cggcctccga ctgcgccgtc
     2401 tcgttctcgt ggtgggggaa cacgaggtct ctcccgcccc cgtggatgtc gagggtgggg
     2461 cccaggtagt gggtgctcat ggcggaacac tcgatgtgcc acccgggcct gcccctggac
     2521 caggggctct cccaccaggg ctcaccctcc ttgctccgct tccacagggc gaaatccagg
     2581 gggtcgcgct tcatctcgtc gatatccacg cgggcgccgg cgatcatctc ctcggtcctc
     2641 ctcccggaga ggccgccgta ggtcctgaac ttggcgaccg agaagtacac gctgccgtca
     2701 ggggcggggt aggcgtagcc cttgtcctgg aggatacgca ccatgtcgat gatctggggt
     2761 atgtggtcgg tcgccttggg ctcgatgtcc gcgttcagca ccccgagcga cttcatgtcc
     2821 gtcgcgaact cctcgatgta ccggtcggcg acctcccggt agtctatgcc gagggtgttg
     2881 gccctcgcga tgatcttgtc gtcgatgtcg gtgaagttcc tcacgtaggt gaggtcgtag
     2941 ccgcaccttc tcaggtacct ggctatgacg tcgaacacga ggagggagcg cgcgtgtccc
     3001 acgtggcaca tgtcgtacac ggtgacgccg cacacgtaca tgccgacctt ctttcctgcg
     3061 agcggctcga agggtttctt tttcttgagg ggcgtgttgt atatggtcag ctgcatgggg
     3121 tctccttata catgcctatg tatgcctgtg ccgtcgggac gaagaaagta ccacacacca
     3181 ccttggcgat aaagggtttt caaggccgtg ggggccgcac tcgtcacgga tgcccgcgtc
     3241 cgggatttca cgccacctcc tccagcagca ccaccgcctg ggccgagacg gcctcgccga
     3301 ggcccacggg accaagtccc tcggaggtcc tgaacttgat cgagaccgcg gaggggtcca
     3361 cccctgccgc cgtcgatatc gccgtgacca tttcccggcg gtggccggag agcctcggcc
     3421 tctcgcagat gcagttgatg tcgatgttgg ctatcctcag gcggccaccg agcatggccg
     3481 cgaccctgcg cagcatctcg agggagtcca tgccctcggt ctcgggcgag gtgtccggaa
     3541 agtgctcgcc gatgtcaccg gcggccaggg ccccgaggat ggcgtcgcag acggcgtgca
     3601 cgaccacgtc cgcgtcagag tgcccgagca ggcccctgtc gtgggggatc ttcacgccgc
     3661 cgatcaccag gggcctgcct gccacgagac ggtggatgtc gtaacctata ccgatcctgc
     3721 acggcatctg agtatcgcct ccgcgagggc caggtcggcc gggtaggtga tcttgatgtt
     3781 gaggggatcg ccgtccacga tcctcactct catgcccgcc tcctccagga gcgagctgtc
     3841 gtccgtgtag gcggcctttt tgtgcctgtg ggccttctgg tgggcctcca ccagcgcatc
     3901 cgtcacgaac gcctgcggtg tctggacgca cacgagcccc gccctgggga ccgtgcgggc
     3961 gacgaacccc tcgcgggcct ccttcaccgt gtcggtcagc ggcaacgccg ggatgcagcc
     4021 gtggcagccc tcgaggcccg agaccacccg tcgagccagc tccggggcga cgagcggccg
     4081 gaccccgtcg tgtacgacca ccgtcccccc cggcctgcag tgcctgaggg cgttgtacac
     4141 cgattccctc ctggttgcgc cgccgggcgt gacgatgagc tcgacggcca tgccctgaag
     4201 gatcccggat gcgcgctcca cgtcctcggg cggcaccgcg acgacgatcc tgtcgcaggg
     4261 caggcacctc tcgaaacact cggcggacct cctgagcacg gggatgccgc ccaggtcgag
     4321 gaactgtttc ctggccccgt agcgcaggcc cgtcccaccg gcgacgatga cgacatcaag
     4381 cggaggcatg gttcttgggc tgtccgaaga tcatcctgcc ggcgggcgtc tggaggatgg
     4441 aggtcacggt cacctcgatg gactggttga tcagggattc cccctgttcc accaccacca
     4501 tggtcccgtc gtcgaggtag gcgaccccct ggccccgttc ctttccgggc ctcgtgatgt
     4561 tcaccgtgag cgtctccccg ggcaggacga tgggcttgac cgcattggcg agctggttga
     4621 tgttgaggac gttgacgccc tgcagctgcg cgatcttgtt gaggttgaag tcgttggtga
     4681 ggatgtcggc gccgatctcg cgcgccaggg cgatgagctt ggagtccacc tcgcggatct
     4741 tcgggaagtc gatgtccatg acctcgatct tcacgcccgg gctcttcctc agcttgttga
     4801 ggatgtcgag gcctcgcctg ccacgcgccc tcttgagggg atcaggcgag tcggctatgt
     4861 gctggagctc gttgaggatg aactgcggcg ccacgatcgt cccctcgatg aagcccgcct
     4921 cgcagatatc cgctatcctc ccgtcgatga tgacgctcgt gtccaggatc ttcatccttg
     4981 cgccctcggt cttcgacctc tggcggaaga tggggagctg ggagatctcc tttccccagt
     5041 tgaggccgat ggtgtaaccc agataggcga aggtggtgtt caccagtatg aagaggtaga
     5101 tgccgctctc gtttatgttg agcaggacgt aaccgaagag gctgccgatg atgaggccca
     5161 ggatcgttcc gacgaggccg ccggcgtacg ccatggggtg gagcttgaac ccccggatct
     5221 cccccaggaa cacggcggcc aggcagctca gcgcgatgat cggcaggatc acgagccaga
     5281 ggccgccctt catctgtacg agtgcgtact ggtaggcgcc gtagaacacg atcacggcta
     5341 tgaggatgcg cagcagccat ttcatcgggc gtcagtctcc gggagatctc tcttcatggt
     5401 acgtgactat ggggtttcgg ttcttccgag ggctgtcagg atcatcttct ccgcttcctt
     5461 cttgtccatc ttgcccgata tggatatctc gttgacgagc aggttcttgg ccgtgtcgta
     5521 catcttgcgt tcgccgaaag agagttcctt ccccttcttc aggatgttca ggtccctgag
     5581 cacctccgct atctcgaagg cggagccgct cttgatcttc tccatatact cgcggtagcg
     5641 cctgttccac gtctggttgt
//
LOCUS       DAOSCR010000093         5600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9141_length_5600_cov_3.343012, whole genome shotgun sequence.
ACCESSION   DAOSCR010000093 DAOSCR010000000
VERSION     DAOSCR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_5600_cov_3.343012"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="PLT69_03670"
     CDS             complement(<1..1670)
                     /locus_tag="PLT69_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOJ13573.1"
                     /translation="MLKPRKVPKWSEYAPPRGTWRSVFKYGSPYEFKHPSLRWVEMIK
                     REFGMKDEDFMSRKDEGLGEVTLERPVALDPVHRERIESIVGRRNVAADAYSRVKYAA
                     GKTTEEIMELRKGVVRACADLVVHPRSKQDVKEIVRYCSEHRIPLSTYGGGTSVTRGL
                     GPEEGGVVLVLSTHMNKVLGVSELNQTARVQAGILGPALEEALRRAPELYHTSHRYTC
                     GHFPQSFEYSSVGGWIVTLGSGQASSYYGDAYHLVLGQEYVTPAGTFVTRDFPAEASG
                     PKINDIFKGSEGAFGVLVEATLRIFRYMPENTRRFSFMFPSWEAGLDACREISQGEFG
                     MPAIFRISDPEETDRGLKLYGMPSFVDSLLRRRGMRPMERCLFIGTSEGQRDFARNVE
                     RQVKRICSSYGAVSLTGYAAKKWEHTRFKEPYMREDLMDFGVLIDTLETSVTWENIKK
                     VHNEVRRYITSRPKTMCMAHASHFYPQGTNLYFIFLARMDDIEEYREFHRGIVDAIVA
                     AGGSVSHHHGVGRLFRPWLRSYLGEGQLQVFRTLKRHFDPAWILNRGVL"
     gene            1873..2055
                     /locus_tag="PLT69_03675"
     CDS             1873..2055
                     /locus_tag="PLT69_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13574.1"
                     /translation="MKVGRHMTGVVGKDLPHARTAELPARLVINCTGPCADITLGKAS
                     SDVPSTSFTFQAPREL"
     gene            complement(2027..3661)
                     /locus_tag="PLT69_03680"
     CDS             complement(2027..3661)
                     /locus_tag="PLT69_03680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOJ13575.1"
                     /translation="MKRFIEEPIPSAYDIVVAGGGITGAAVAYEAACRGFRTALVEKK
                     DFAWATSSATSKLIHGGLRYLANAEFGLVRESLKERSVLQNIAPNLVHPIPMMMIHDN
                     VCPKNKPFEIAVGLMLYDLLAYDRNKTWDPVKKIPQHRRMTAEEALVLEPGIFARGLK
                     GASVFYDCANAFPERLTLAFIKSAHVHGAHVANYAEVASFMKEGPRVTGVVVKDLLHA
                     RTVEIPARLVINCTGPWADITLGKASSSVAAAPLTRSEGIHIVTRRRVNTHVVSYMTA
                     GGRHFFMIPWRNHTLIGTTDEPYTGSPDDYRVTASSIKGLIDEVNETVGAEVVDVDDV
                     LYAYGGLRPLVEDVSADTYGASRRYEIQDNAKDGIDGLVTVEGGKFTTSRMLAEKTID
                     IAETKLEKRHTPSTTAKRYLWGCQIPDMRTFGLDVFKKNRDFDSDTLEYLVRNYGTET
                     EAVLELARNDPDLRERMDKDGEIAAQVVYAMRNEMARSLEDIVLRRTGIASLGDPGRD
                     ALRSAADIAATELGWDGERKEREISEVLRVLAVPGR"
     gene            complement(3780..4571)
                     /locus_tag="PLT69_03685"
     CDS             complement(3780..4571)
                     /locus_tag="PLT69_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOJ13576.1"
                     /translation="MARYMTIPGKKEASQLQRTRLFERVAAILLKRILKGGYAVGLRL
                     PTERELATSFDVNRSTVREALKRLESLGVIEIRHGDGVYVKDYMESPSLDLIWAMFYL
                     DDRLDADILMTLLEIRRILVPEMAALAAMRRDVSHVKTLEEVASNHQGVSVLERDMLV
                     HRVIARASANTLYQVLINFFDRFFLEFGHLYFDDEANAARSVRFHREIYEAVRDADPD
                     RARTVMREVLDYAERAISRKLEYFGGTDPGVKIRMGEKDAGTARD"
     gene            4960..5265
                     /locus_tag="PLT69_03690"
     CDS             4960..5265
                     /locus_tag="PLT69_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOJ13577.1"
                     /translation="MVTIMVQVRVKPEFVDAFVEETKKNAENSLLEPGVLRFDILRHA
                     LDPAAFLLVEVYRTEQDPARHKETPHYKAWRSAVEHMMAEPRSSSQYVKVFPPEEDH"
     gene            5262..>5600
                     /locus_tag="PLT69_03695"
     CDS             5262..>5600
                     /locus_tag="PLT69_03695"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ13578.1"
                     /translation="MRFETVRFEFVLPGRIVFGNGCSGDLPSLAKEMGSRCILVTGSS
                     PERLHGLVEELGSSGILGRVFRVEREPTTDDVRRGVLEARQAECDLVVSIGGGSAIDT
                     GKAVSAMLTNP"
BASE COUNT          915 a         1844 c         1664 g         1177 t
ORIGIN      
        1 aggacgcccc tgttcagtat ccaggcgggg tcgaagtgcc tcttcagcgt cctgaacacc
       61 tggagctgtc cttcccccag gtaggacctg agccatggcc tgaagagcct gcccacacca
      121 tggtggtggc ttacggaccc gcctgcggcc acgatggcgt ccacgatccc cctgtggaac
      181 tcacggtact cctcgatgtc gtccatcctc gcgaggaaga tgaagtagag gttggtcccc
      241 tgtgggtaga agtgcgaggc gtgggccatg cacatggtct tcggcctgga cgtgatgtac
      301 cgcctcacct cgttgtgcac cttcttgatg ttttcccagg tcaccgaggt ctcgagcgtg
      361 tctatgagaa cgccgaaatc catgagatcc tcgcgcatgt agggctcctt gaagcgcgta
      421 tgctcccact tcttcgccgc gtacccggtg aggctcacgg cgccgtacga tgagcagatc
      481 cgcttcacct ggcgctcgac gttcctggcg aagtccctct gcccctcgga ggtgcctatg
      541 aacaggcagc gctccatcgg cctcatgccc cgcctcctga gtagggagtc cacgaacgac
      601 ggcatgccgt agagtttcag cccccggtcc gtctcctccg ggtcggagat cctgaatatc
      661 gccggcatcc cgaactcgcc ctggcttatc tcgcggcagg cgtcgagccc ggcctcccag
      721 ctcgggaaca tgaagctgaa tcttctcgtg ttctcgggca tgtacctgaa gatcctcagg
      781 gtcgcttcca cgagcacgcc gaacgcaccc tcgctgccct tgaagatgtc gttgatcttc
      841 gggcccgagg cctcggccgg gaagtccctc gtcacgaagg tgccggcggg tgtgacgtac
      901 tcctggccga gcaccaggtg gtatgcatcg ccgtagtagg aagacgcctg tcccgacccg
      961 agcgtcacga tccagccacc taccgacgag tactcgaagg actgcgggaa gtgcccgcac
     1021 gtgtagcggt gggacgtgtg gtagagttcc ggggccctgc gcagcgcctc ctcgagggcc
     1081 gggccgagga tgccggcctg tacccttgcc gtctggttga gctcgctcac cccgagcacc
     1141 ttgttcatgt gcgtggagag caccaggacc accccaccct cctccgggcc gagccctcgt
     1201 gtcacggagg tgcccccgcc gtaggtagac aggggtatcc tgtgctcgct gcagtaacga
     1261 acgatctcct tgacgtcctg cttcgaccgg gggtgcacga cgaggtcggc gcatgcgcgc
     1321 acgaccccct tcctcagttc catgatctcc tcggtggtct tgccggcggc atacttgacc
     1381 ctgctgtacg catccgccgc gacgttgcgc ctgccgacga tcgactcgat gcgttccctg
     1441 tgcacggggt ccagggcgac cggcctctcg agggtgacct caccgaggcc ctcgtccttt
     1501 ctcgacatga agtcctcgtc cttcatgccg aattcccgct tgatcatctc cacccaccga
     1561 agagacgggt gcttgaactc gtaggggctc ccgtacttga acacggaacg ccatgtcccc
     1621 ctgggcggcg cgtactccga ccacttcgga acctttctcg gtttcagcat gttcacgtct
     1681 cctcatgcat actcgagcgc gcctctcgcc acgggcgtcg ctcttttagg acgatggtgg
     1741 ccgtttcctc tcaacggccc tcctttatcc tggaaatcaa cggggatcct cgaacgcagg
     1801 aggactcccg ggcgcatcca ggccttttcc ttcccggcgc ccaagggttt cccctttgag
     1861 gtggtgtcct tcatgaaggt aggccggcac atgaccggcg tggtgggaaa ggacctcccc
     1921 catgcacgga ccgccgagct acctgcccgg ctcgtcatca actgcaccgg gccatgtgcg
     1981 gacatcaccc tggggaaggc atccagtgat gtaccgtcca cgtcattcac cttccaggca
     2041 ccgcgagaac tctgagcacc tctgagatct ccctttcctt cctctcgccg tcccagccga
     2101 gctcggtcgc ggctatgtcc gcagcagagc ggagggcgtc ccttcccgga tcgccgaggg
     2161 atgcgatccc agtgcgcctg agcacgatgt cctcgaggct tcgcgccatc tcgtttctca
     2221 tggcgtagac cacctgtgca gcgatctcgc cgtctttgtc catcctctcc ctgagatcgg
     2281 gatcgttcct cgccagctcg agcacggcct cggtctcggt gccgtagttc ctcacgaggt
     2341 actcgagggt gtcgctgtcg aagtccctgt tcttcttgaa gacatcgagg ccgaacgtgc
     2401 gcatgtcggg gatctggcac ccccagagat acctcttggc ggtcgtcgag ggggtgtgtc
     2461 tcttctcgag ctttgtttcc gcgatgtcga tggtcttttc cgcaagcatc ctgctcgtgg
     2521 tgaactttcc gccctcgacg gtgaccagcc cgtctatgcc gtccttggcg ttgtcctgga
     2581 tctcgtacct tcgggaggcg ccgtaggtgt ccgccgagac gtcctcgacc aggggcctca
     2641 ggcctccgta ggcgtacagg acgtcgtcga cgtccaccac ctctgcgccg acggtttcgt
     2701 tgacctcgtc tatgagcccc ttgatgctgg aggccgtcac ccggtagtcg tcgggcgagc
     2761 ccgtgtacgg ttcgtcagtg gtcccgatga gggtgtggtt tctccagggg atcatgaaga
     2821 aatgcctgcc gcctgcggtc atgtaactca ccacgtgggt gttcacacgc ctcctcgtca
     2881 cgatgtgtat gccctccgag cgggtcagcg gcgcggccgc caccgagctg gacgccttcc
     2941 ccagggtgat gtccgcccat ggcccggtgc agttgatgac gagccgggcg ggtatctcga
     3001 cggtccttgc atgcaggagg tccttcacca ccacgccggt tactcgaggg ccttccttca
     3061 tgaaggacgc cacctctgcg tagttcgcga catgggcgcc gtgaacgtgc gcggacttga
     3121 tgaaggcgag cgtcaggcgt tcgggaaagg cgttcgcgca gtcgtagaag accgatgcgc
     3181 ccttgagccc cctcgcgaag atcccgggtt ccaggaccag ggcttcttcg gccgtcatcc
     3241 tccggtgctg ggggatcttc ttgaccgggt cccaggtctt gttcctgtca taggcgagga
     3301 gatcgtacag catgagcccc acagctatct cgaagggctt gttcttcggg cacacgttgt
     3361 cgtggatcat catcatgggg atggggtgaa cgaggttcgg cgcgatgttc tggagtacgc
     3421 tccgctcctt cagcgactcc ctcacgaggc cgaactcggc gttcgcaagg tagcgcaacc
     3481 ccccgtgtat gagcttcgac gtggccgagg aggtcgccca tgcaaagtcc ttcttttcca
     3541 cgagggcggt cctgaacccc ctgcatgcgg cctcgtacgc gaccgccgca cccgtgatgc
     3601 ccccgcctgc gacgacgatg tcgtacgccg acggtatggg ctcttctatg aatctcttca
     3661 tgtcatccct tccttttctt cttcgagggc ctccgcttca agcaaggacg cgaatctcag
     3721 cgaccatgcc ggtctcccgc ggtcttcgcg ccgtgccggc atcgaggtcc acggcgtcct
     3781 caatccctag ctgttccggc atctttctct cccatgcgta tcttcacccc tggatccgtc
     3841 ccgccgaaat actcgagctt ccggcttatg gcacgctctg catagtcgag cacctcccgc
     3901 atgacggtgc gcgcccgatc agggtccgcg tccctgacgg cctcgtagat ctcccggtgg
     3961 aacctcaccg acctcgccgc gttcgcctcg tcgtcgaagt agaggtgtcc gaactcgagg
     4021 aagaacctgt cgaaaaagtt gatcagcacc tggtagaggg tgttggcgct cgccctcgct
     4081 atgaccctgt gcacgagcat gtccctctcg aggacgctca ctccctggtg attcgatgcg
     4141 acctcctcga gggtcttcac gtgggacaca tccctcctca tggctgcaag cgcggccatt
     4201 tccggcacga ggatgcgcct gatctcgagc agggtcatga ggatgtcggc gtccaggcgg
     4261 tcgtccaggt agaacatggc ccagatgaga tcaaggctgg ggctttccat gtagtccttc
     4321 acgtagaccc cgtcgccgtg cctgatctct atgacgccca gagactcaag cctcttgagg
     4381 gcttccctca cggtggacct gttcacgtcg aaagaggtgg cgagttccct ctccgtcggc
     4441 aggcggaggc ccaccgcata cccccccttg agaatgcgct tcaggaggat cgccgcaacc
     4501 ctctcgaaca gcctggtcct ctggagctgc gacgcctcct tctttcctgg tatggtcatg
     4561 taccgtgcca tggcttaatt ggtcgggtgg tcggaccaat tacacatcac ctgtcgcctc
     4621 ttgtcaacaa aaaacacgtc atgaccatgg acggcagtgc acgatgagca gccagtccct
     4681 ttgatggaga gtcggccccg cctcccgggc atgtcgtctc ttcggactcc cccctttatg
     4741 gatacacccc atctttgtcc cagggaacgc atcttaaggg gtcgccgcat cgctcaagcc
     4801 cctcccgctt gcctcgagtc cccattgtgg tgtacgtgct gtgtgtactc gtgaggcgcc
     4861 aagaagcgct cccctgctcc ggcgatcctg ccgtccagca gaaacgcctc gaggtttctt
     4921 gggcagcggg ggacgatcct ctgtcaacgg gggtgatcga tggtcacgat catggtccag
     4981 gtgcgagtca agcccgagtt cgtggatgcc ttcgtggaag aaaccaagaa gaacgccgag
     5041 aacagcctgc ttgagccggg ggtcctgagg ttcgacatcc tcaggcacgc cttggacccc
     5101 gccgccttcc tgctcgtcga ggtttaccgc acggagcagg acccggcaag gcacaaggag
     5161 acaccccact acaaggcctg gcgctctgcg gtcgagcaca tgatggcaga gccgaggtcg
     5221 agttctcagt acgtcaaggt cttccccccc gaagaagacc attgaggttt gagaccgtgc
     5281 gtttcgagtt cgtcttgccg ggaaggatcg tcttcgggaa cgggtgctcg ggcgacctcc
     5341 cgtctctggc caaggaaatg gggtcgcggt gtatcctcgt gaccggcagc tcccccgagc
     5401 gtcttcacgg ccttgtcgag gagctgggct cgtcggggat cctgggccgt gtcttcaggg
     5461 tggagcgtga gccgaccacc gatgacgtga gacggggcgt cctcgaggcg aggcaggcag
     5521 agtgcgacct cgtcgtatcc atcggcgggg gcagcgcgat cgatacgggc aaggccgtct
     5581 cggccatgct gacaaacccc
//
LOCUS       DAOSCR010000094         5583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9173_length_5583_cov_3.488242, whole genome shotgun sequence.
ACCESSION   DAOSCR010000094 DAOSCR010000000
VERSION     DAOSCR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9173_length_5583_cov_3.488242"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..443
                     /locus_tag="PLT69_03700"
     CDS             54..443
                     /locus_tag="PLT69_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13579.1"
                     /translation="MTSVGDIVVVHVSDKPSVYARIEAVEPDVKAGWFRVKLLLLTSP
                     PREVIWILRREYIEGARFTMQDVPMHLSRLQAPSGRQPLDEAGTRSRPKVIPLRKAAR
                     SREGDDEPTEGSDHTILNAPPTEPGRS"
     gene            540..2243
                     /gene="pilB"
                     /locus_tag="PLT69_03705"
     CDS             540..2243
                     /gene="pilB"
                     /locus_tag="PLT69_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOJ13580.1"
                     /translation="MANERIGEQLVKNALITPEQLLEAQRAQKTTGTRLGSQLVKLGH
                     LSEEQLVEFLGKQYRLPSIILKNMNPDPEVVKLIPLNVVQKYHAIPFDRNGSTLKVAM
                     TDPTNIFAVDDLKFLTGYSIEVYVTSEDSLKWAIDHFYDQTASLDDALSSIEDLGGAV
                     EVSMDGDEISVGDLEKEADQAPVIKLVNLTLLDAIKKGASDIHMEPYEKFMRIRYRLD
                     GMLIEAMKPPLKLKNALVSRIKIMAKLDIAERRLPQDGRIKLKVQGGKEVEFRVSVLP
                     TLFGEKVVLRILDKSNLQLDMTKLGFEEEALARFKEAIYKPWGMVLVTGPTGSGKTTT
                     LYSALSELNKIDRNISTAEDPVEFNLPGVNQVQVHEEIGLTFAAALRSFLRQDPDIIL
                     VGEIRDFETAEIGIKAALTGHLVLSTLHTNDAPSTVNRLLNMGIEPFLVASSVNLIVA
                     QRLARKVCTQCRTKDEVSVETLHQMGMDLETAGRVECITGKGCPMCGNTGYKGRIALY
                     EVMPITESIRELILMGASATEIKNQAVKEGMKTLRQSGLTKIVEGVTSVAEILRTTMA
                     D"
     gene            2265..3368
                     /locus_tag="PLT69_03710"
     CDS             2265..3368
                     /locus_tag="PLT69_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ13581.1"
                     /translation="MEHSIYDLLKMMVDKGASDLHVTTGSPPQVRVRGQLYPLDSPVL
                     DPKSVRELLYSILTEAQKHKFEEEQELDLSFGIKGIARFRANIFVQRGALAGVFRMIP
                     YEIMPLDGLGLPPVVMKLTSLPRGLILVTGPTGCGKSTTLAAFLDKINTERHDHIVTI
                     EDPIEFVHKHKGCLVNQREIGSDSKSFANALKYILRQDPDVVFIGEMRDLETMQAALT
                     ISETGHLVFATLHTNSAVQTVNRIVDAFPAHQQSQIRAQLAFVLEAVIAQQLIPRLDG
                     KGRVLASEVMISTPAIRNLIREDKIHQIYSQMQVGQTKHGMQTMNQSLLNLYLRKLIS
                     LDDALGRSLDPEELRQMMSSPSHQRSTTGMERR"
     gene            3372..4565
                     /locus_tag="PLT69_03715"
     CDS             3372..4565
                     /locus_tag="PLT69_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ13582.1"
                     /translation="MAVFVWEGRLVDGTVKKGEIEAADKAVVGMILKRQRIVPTKIKA
                     KPKELTLFTRGVKTKEIVIFTRQFATMISAGLPLVQCLDILSTQQPNPSFKKVLSQVK
                     QDVESGSTFADALRKHPKVFDSLYVNLVAAGEVGGVLDTVLNRLAVYMEKNENLKNKI
                     KSAMTYPIIVLCVAFGVVAVLMIFVIPTFSDMFKQFGSALPAPTQLVVNLSNLFRHFW
                     WAIFGAIVGFVIAFKWVRTQKKGRYMTDKLFLRLPIFGPLLRKVAVAKFTRTLGTMIS
                     SGVPIMDGLEITSRTAGNVIVEEAILAVRASISEGKSMSEPLEQTGIFPGMVVQMISV
                     GEATGAMDQMLGKIADFYDEEVDAAVEALTSALEPMLMVFLGGIIGFVVVAMYLPIFQ
                     MAQNV"
     gene            4575..4844
                     /locus_tag="PLT69_03720"
     CDS             4575..4844
                     /locus_tag="PLT69_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13583.1"
                     /translation="MSVYEAVMLICFGSAWPVSIYKSAVSRTAKGKSAVFLFIVLAGY
                     VAGILHKVHYNLDWVTALYAINGLMVLADIVLTLRNVLLDRRSRA"
     gene            complement(5064..>5583)
                     /locus_tag="PLT69_03725"
     CDS             complement(5064..>5583)
                     /locus_tag="PLT69_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOJ13584.1"
                     /translation="RTELKRMLEKGDLAHACEEAARRQIEAFFATGMAPSHLDTHHHV
                     HGFRPVFDLVVRLALEYGIPALRFSRGGYRLPTRQDIPLQPEEALAMEEALTRAGIFH
                     CDRMAEGAGLIFGFMDEPPEDTTELVVHPSLGGDPWRARELDALERAARGRPLRGPGF
                     VLTSFRDLVGLE"
BASE COUNT         1132 a         1810 c         1711 g          930 t
ORIGIN      
        1 cgcaccatcg agacgggatg acatccgttg ccactttgtg gtagacaagg cccatgacct
       61 ccgtagggga catcgtggtc gtccacgtga gcgacaagcc ctccgtctac gccaggatcg
      121 aggcggtcga gcctgacgtg aaggcaggct ggttcagggt gaagctcctc ttgttgacct
      181 cccccccccg cgaggtcatt tggatactca ggagggagta catcgagggg gcgcggttca
      241 ccatgcagga tgtgcccatg cacctctccc gcctccaggc gccttccggc agacagcccc
      301 ttgacgaggc gggcacccgc tcgaggccga aggtgatacc cctcaggaag gccgcacgat
      361 cccgggaggg agacgacgag cccaccgaag ggtctgacca cacgatcctc aatgcacccc
      421 ccacggaacc ggggcggtcg taacatctca taagaacgca cgattacacc attgataacg
      481 gtggatggtc cgttaaagtc ggcatatcca catgccgata ggctaacaga aggtgaaaca
      541 tggcgaacga gcgcataggc gagcaacttg tcaagaacgc actgatcacc cctgaacaac
      601 tcctcgaggc gcaacgcgcc cagaaaacca cggggacccg gctcggatcc cagctcgtca
      661 agctcgggca cctgagcgag gaacagctcg tcgagttctt gggcaagcag taccgcctgc
      721 cctccatcat actcaagaac atgaaccccg acccggaggt ggtcaagctc ataccgctga
      781 acgtggtgca gaagtatcac gccatcccct tcgaccggaa cgggtccacc ctcaaggtgg
      841 cgatgaccga ccccacgaac atcttcgccg tcgacgacct caagttcctc acggggtatt
      901 ccatcgaggt ctacgtcacc tccgaggact ccctcaagtg ggccatcgac cacttctacg
      961 accagaccgc ctccctcgac gacgccctga gcagcatcga agacctgggc ggggccgtcg
     1021 aggtgagcat ggacggcgac gagatatcgg tgggagacct cgagaaggag gccgaccagg
     1081 cgcctgtcat caagctcgtc aacctcacgc tcctggacgc catcaagaag ggcgcgagcg
     1141 acatccacat ggagccgtac gagaagttca tgcggataag gtaccggctt gacggcatgc
     1201 tcatcgaggc catgaagccg cccttgaagc tcaagaacgc gctcgtctcg cgcatcaaga
     1261 tcatggccaa gctcgacatc gcggagcgga ggctccccca ggacggcagg atcaagctca
     1321 aggtccaggg gggcaaggag gtggagttcc gcgtgtcggt gctcccgacc ctgttcggcg
     1381 agaaggtggt gctgcgtatc ctggacaagt cgaacctcca gctcgacatg acgaaactcg
     1441 ggttcgagga ggaggcgctc gcccgtttca aggaggccat ctacaagccc tggggcatgg
     1501 tgctcgtcac cgggcccacc gggtcgggaa agaccacgac cctgtattcc gccctgtcgg
     1561 agctcaacaa gattgaccgc aacatctcca cggccgaaga ccccgtcgag ttcaacctcc
     1621 cgggcgtgaa ccaggtccag gtgcacgagg agatcggcct caccttcgcc gcggccctgc
     1681 ggtccttcct gaggcaggac ccggacatca tcctggtggg cgagatacgc gacttcgaga
     1741 ccgccgagat agggatcaag gcggccctca ccggccacct cgtgctgtcg accctgcaca
     1801 ccaacgacgc cccctccacg gtgaacaggc tcctgaacat gggcatcgag ccgttcctgg
     1861 tggcatcctc ggtgaacctc atcgtggcac agaggctcgc ccggaaggtg tgcacccagt
     1921 gcaggacaaa agacgaggtg tccgtggaga ccctccacca gatggggatg gacctcgaga
     1981 ccgccggtcg cgtggagtgt atcacgggca aggggtgccc gatgtgcggc aataccgggt
     2041 acaagggcag gatcgcgctc tacgaggtga tgcccatcac cgagtccatc cgcgagctca
     2101 tcctcatggg cgcatccgca acggagatca agaaccaggc ggtcaaggag gggatgaaga
     2161 ccctcaggca gagcggtctc accaagatcg tcgagggggt cacctcggtg gccgagatcc
     2221 tcaggaccac catggcggac tgacgtcggc aacggaggtt aaccttggaa cattccatat
     2281 acgacttgct caagatgatg gtggacaagg gggcatcgga cctccacgta accacgggtt
     2341 ccccgcccca ggtccgcgtg cggggccagc tctacccgct cgattccccg gtgctcgatc
     2401 ccaagagcgt ccgtgagctg ctctactcga tcctcaccga ggcccagaaa cacaagttcg
     2461 aggaagagca ggagctcgac ctctccttcg gcatcaaggg gatagcgcgc ttcagggcca
     2521 acatcttcgt gcagcgcggg gcgctggccg gcgtgttcag gatgatcccc tacgagatca
     2581 tgccgctcga cggcctgggc ctgccccccg tggtcatgaa gctcacctcg ctgcccaggg
     2641 gtctcatcct ggtcaccggc ccgaccgggt gcggcaagtc cacgaccctg gccgcctttc
     2701 tcgacaagat caacaccgag cgtcacgacc acatcgtgac catcgaggac cccatcgagt
     2761 tcgtgcacaa gcacaaggga tgcctggtca accagcgcga gatcggctcg gacagcaaga
     2821 gcttcgccaa cgccttgaag tacatcctca ggcaggaccc ggacgtggta ttcatcggcg
     2881 agatgcgcga cctcgagacc atgcaggccg cgctcaccat ctcggagacc ggccacctcg
     2941 tgttcgccac cctccacacc aattccgcgg tccagacggt gaacaggatc gtggacgcct
     3001 tccctgcgca ccagcagtca cagatcaggg cccagctcgc cttcgtgctc gaggcggtga
     3061 tcgcccagca gctcatcccg aggctcgacg gcaagggcag ggtgctcgca tccgaggtca
     3121 tgatatccac cccggccatc aggaacctga tcagggaaga caagatccac cagatctatt
     3181 cccagatgca ggtcggacag acgaaacacg gcatgcagac gatgaaccag tcactgctca
     3241 acctgtacct gcgcaagctc atctcgctcg atgacgccct cggcaggtcg cttgaccccg
     3301 aagagctcag gcagatgatg tcctcgccct cgcatcagcg cagcactacg ggtatggaga
     3361 ggagatagcc catggcagta ttcgtgtggg aaggaaggct cgtagacggt accgtcaaga
     3421 aaggcgagat cgaggccgct gacaaggcgg tggtgggcat gatcctcaag agacagcgca
     3481 tcgtccccac caagatcaag gcgaagccca aggagctgac cctgtttacg cgcggcgtga
     3541 agaccaagga gatcgtgatc ttcacccggc agttcgcaac catgatcagt gcaggtctgc
     3601 cgctcgtgca gtgcctggac atcctgtcca cgcagcagcc gaacccctct ttcaagaagg
     3661 tgctctccca ggtgaagcag gacgtggagt cgggaagcac cttcgccgac gccctcagga
     3721 agcacccgaa ggtgttcgac agcctgtacg tgaacctggt ggcggcgggc gaggtgggcg
     3781 gcgtgctcga caccgtcctg aacaggctgg ccgtctacat ggagaagaac gaaaacctga
     3841 agaacaagat caagagcgcc atgacgtatc ccatcatcgt gctgtgcgtg gccttcggcg
     3901 tggtggcggt gctcatgatc ttcgtgatcc cgaccttcag cgacatgttc aagcagttcg
     3961 gttcagccct cccggccccg acccagctgg tggtgaacct gagcaacctc ttcaggcatt
     4021 tctggtgggc gatcttcggg gccatcgtcg ggttcgtgat cgcattcaag tgggtgagga
     4081 cccagaagaa gggcagatac atgacggaca agctcttcct gcgcctgccc atcttcgggc
     4141 ccctgctcag gaaggtggcg gtcgcgaagt tcacccgcac cctgggcacc atgatctcct
     4201 cgggcgtgcc catcatggac gggctcgaga tcacctcgag gaccgcgggc aacgtgatcg
     4261 tggaggaggc gatattggcg gtgagggcgt ccatcagcga gggcaagtcc atgtccgaac
     4321 ccctcgagca gaccggcatc ttccccggca tggtcgtcca gatgatctcg gtgggtgagg
     4381 cgacaggcgc catggaccag atgctcggga agatagccga cttctacgac gaggaggtcg
     4441 acgcggcggt ggaggcgctg acctcggccc tcgagcccat gctcatggtg ttcttgggcg
     4501 gcatcatcgg tttcgtggtc gtcgccatgt acctgcccat cttccagatg gcgcagaacg
     4561 tctgagacgg cggcgtgagc gtctacgagg cggtcatgct gatctgcttc gggtcggcat
     4621 ggccggtttc catctacaag tccgcggtgt ccagaaccgc caagggcaag agcgcggtct
     4681 tcctcttcat cgtgctggct ggctacgttg cgggcatcct gcacaaggtc cactacaacc
     4741 tggactgggt cacggccctc tacgcgataa acggcctcat ggtgctcgcc gacatcgtcc
     4801 tcaccttgag gaacgtcctc ctggacaggc gaagtcgggc ctgagggcgg ctcgaaggct
     4861 ctcatccccg gtctcgggct gatccccggt ctcctccagc actcagcaga gggcatgggc
     4921 gctctcccga ccctcccctt ttctgagcag cggcttttct ccgtctccca caaggcaggg
     4981 ccggcgcacc aaccaaaccc gcccttcagg gcgtgcctcg aggtgggtgc aaaggcccgg
     5041 cacaccgtct gcgggcgcgt gcatcactcc aggccgacga gatcccggaa actcgtgagg
     5101 acgaaccccg gccccctcaa cggccggccc cgggccgcgc gttcgagggc gtccagctcg
     5161 cgggcacgcc aggggtcgcc cccgagcgag gggtgcacga cgagctccgt ggtgtcctca
     5221 ggcggctcgt ccatgaaccc gaagatcagt cccgcacctt cagccatccg gtcgcagtgg
     5281 aagataccgg cccgcgtgag cgcctcttcc atggcgaggg cctcttccgg ttggaggggg
     5341 atgtcctgcc tcgtggggag gcggtacccc ccccggctga acctgagcgc agggatcccg
     5401 tactcgagcg caagcctcac gacgagatcg aagacgggcc tgaacccgtg cacgtggtgg
     5461 tgggtgtcca ggtgtgaggg ggccatccct gtcgcgaaga aggcctcgat ctgcctcctc
     5521 gcggcctcct cgcacgcgtg cgcgagatcc cccttctcca gcatccgctt gagctcggtc
     5581 cgg
//
LOCUS       DAOSCR010000095         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9327_length_5509_cov_3.183718, whole genome shotgun sequence.
ACCESSION   DAOSCR010000095 DAOSCR010000000
VERSION     DAOSCR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9327_length_5509_cov_3.183718"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..394
                     /locus_tag="PLT69_03730"
     CDS             194..394
                     /locus_tag="PLT69_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13585.1"
                     /translation="MIKIGDQDRDASGLKPEDLVCYCFRITKRQIEEDFIRNGRSLVL
                     DKIKEAKKEGGCDCARLNPRGR"
     assembly_gap    545..554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(755..1045)
                     /locus_tag="PLT69_03735"
     CDS             complement(755..1045)
                     /locus_tag="PLT69_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOJ13586.1"
                     /translation="MKMLLMVYDAEFDDEVMAALARLPLAGYTKWDRVLGKGRRSEPK
                     LDDAVWPGFNCAVAVAVDDETEEAAFRELQGLFTRLGGKGFSVFELPVLKVI"
     gene            complement(1042..4155)
                     /locus_tag="PLT69_03740"
     CDS             complement(1042..4155)
                     /locus_tag="PLT69_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ13587.1"
                     /translation="MKLPEFSVRQPVATLMVFCAVILMGTVSLLYMSTDMFPDVEPPV
                     ITVITTWPGANASDVETEVTDVIENQLNGVANLDSITSKSMDNISLIACKFEWESDLD
                     VASNDIRDRLEFAKKDLPADIDPPMLFKFSSATAPILMMTVTGEKSWPRLHHIVDNRI
                     ADELKRVPGVGAVMLVGGLVRRINVDFDPERLEGYNLSVQQVNQVLSAQNLNIPAGSI
                     KAGGKEYFLRVPGRYTSPAEIGETIVGSYGGRPVRLRDVATVGDSYEDETMYGWGNGK
                     RAIAMVLQKQTGANTVEVVRAVKKKLDSIRQNLPSDVRVNINVDSSATILNSIANLRK
                     TLIEAIVIVAVVTVAFLRRVRTSFIICTAIPFSLIAAFIVLYLFGYTVNMVLLMSMAI
                     AVGMVVDDAIVILENIVRHLERGAKPALGSIFGAGEMGMAVTASTFTVAVVFIPLIFV
                     SGLTGVFFKVFAITMVVTILASLFSSLTMTPMLASRLLSGGEAAPPGNRGLAGVAYRA
                     SERWFEALEQAYGRLLAWALAHTRAVVVTAVVVFGSGLALVPFLATSFVPQMDTGDIT
                     IGVRMPEGTRIEETKKVIEGIHAIVGKTIRPGELLHVFSFCGQTKEGFGEAVGFEEAP
                     NAGQVFIKLVDRQERSRSAKEIANELRRSITGIPGISSIKILAQDPMTSVIMGGQKPI
                     VVEIQGDDLKECLAYASRLGEETRSIPGVIDLTLSQKNDRPELQVRIDRTKASTLGLT
                     AGQVAAMLRNYYHGQAATSFWDTGERYDIYTRYTVENKNDPDELMNAPLFTSDGRVVR
                     LANVATITDGSGPVEIQRKNRQKIVRLEADIYKRELGEVTADIKAVMDRLGIPPGLSA
                     RFGSDVEEQAKAFKDLSILMLLGVVLVYMVLASLYGNLRDPLIIMFSVPFGIVGVVYA
                     FFLFKVPLDLMTFMGVVMLSGIVVKNAIVLLDYTHLLLKRGRTLEEAVTTAGSHRLRP
                     ILMTTLTTVLGMVPMALSKGQGAEFWNPLGITMISGLSVSTLITLVLIPTVFYGLESR
                     KQGRKAS"
     gene            complement(4156..5265)
                     /locus_tag="PLT69_03745"
     CDS             complement(4156..5265)
                     /locus_tag="PLT69_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ13588.1"
                     /translation="MQTQSGPRPRKAVLLLVILAAACAGLFAWRVHSGVSDRDEAPAS
                     EKVPVSTVPAAIGNVERVLRLTGDVRPWREVDVFPKVPGKIIERIFFDRGDTVRSGDT
                     IALLEMRSIEAQVREAEAAFRLAGTNLDLTRKDYARIENLYAEKAVARQRLDHVQAQL
                     ESAEAQLDRSKAVLDQLRILMAEHTVRSPIGGVISARYVDPGALSAPGAPIVRVSDES
                     RLKIVASVTEKDYLSIAKGMRCEVTSDAVPGAVFEGEIALVNPTLSPSGRSADIEIHV
                     DNAKKGLKAGMFAHVRISLGVVSGVVVEKDAVQRLPGTGAPYVYVVEDGKAVMKNIET
                     GPSQGDVVLVASGLAPGERVVVTGQRPAPREPGVQ"
     gene            complement(5270..>5509)
                     /locus_tag="PLT69_03750"
     CDS             complement(5270..>5509)
                     /locus_tag="PLT69_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ13589.1"
                     /translation="IASAREALEQARENYRITNLQYRQQITSSTEVLDARTYLSQAQM
                     NYYGALYGYLSAIARLERASGVTLSGPQPNTNGGS"
BASE COUNT          922 a         1778 c         1792 g         1007 t
ORIGIN      
        1 ctgcatgatg cctacatcgc agatgccaat gtacagaaaa tgggggactt gactgctgtg
       61 aatgctcagc gcctatgtcg gatagtatat tccacaggtg ataggagact tgaccctgga
      121 cacgccttgc cgcatggggg gtggtcaggt atagtgcatg tcggtcacac acgttataga
      181 tgcgggaagg cgcatgatca agatcggaga tcaagaccgg gatgcatccg ggctcaagcc
      241 ggaagacctg gtgtgctatt gtttccggat cacgaagagg cagatcgagg aagacttcat
      301 ccggaacggc cgctcgcttg tcctcgacaa gatcaaagag gccaagaagg agggcgggtg
      361 cgactgcgcc cggctgaacc cgcgtgggcg ttgatgcctg gatgatgtcc gccgggtggt
      421 ggacaccatc tcgaggaagg ccccctgaag ccggcaccgg caagcccagc cgtccgcgcc
      481 tgagcggtcc cgtggcaccg cgcgggttgt ccgcaagggc cccttgcctg gcgcgaagac
      541 ggtgnnnnnn nnnncatcga gaacgaacag ttccggattg ccccgctcga ccacgagggc
      601 ccctcccctg gagcgaagac ggcatagccc gggcggttgc gcctgaatga tcatacccaa
      661 gggcgcgcgg ggcggctcca gaagtgggtt ttacgagcag atagcctgtt gcgcctgaat
      721 gatcataccc acggggcggg cggggccatg cctgtcagat gaccttgagc acgggcagtt
      781 cgaacacgga gaagcccttg ccgccgagcc tggtgaagag cccttgcagt tccctgaagg
      841 ccgcttcctc ggtctcgtcg tccacggcaa cggccacggc gcagttgaac cccggccaca
      901 cggcgtcgtc gagcttgggc tccgagcgcc tgcccttgcc caggaccctg tcccacttgg
      961 tgtacccggc aaggggaagc cgtgcgagcg cggccatgac ctcgtcgtcg aactccgcgt
     1021 cgtataccat caggagcatc ttcatgaggc tttcctcccc tgtttcctcg actcgaggcc
     1081 gtaaaagacc gtgggtatga gcacgagcgt gatcagggtg gacacgctca ggccggagat
     1141 catggtgatg ccgagggggt tccagaactc tgcgccctgg cccttggaca gcgccatggg
     1201 caccatgccc agcacggttg tcagcgtggt catgaggata ggcctgagcc tgtgtgaacc
     1261 cgccgtggtc accgcctcct cgagcgtcct gccgcgcttg agcaggaggt gcgtgtagtc
     1321 gagcagcacg atcgcgttct tcaccacgat gcccgagagc atcaccacgc ccatgaaggt
     1381 catgagatcg agaggcacct tgaagaggaa gaaggcatac acgacgccca ctatgccgaa
     1441 gggcaccgag aacatgatga tgagcgggtc cctgaggttg ccgtagaggg atgcgagcac
     1501 catgtacacg agcaccacgc ccaggagcat gaggatcgag aggtccttga acgccttggc
     1561 ctgttcctcc acgtccgacc cgaagcgcgc ggacaggccg ggcgggatgc cgagcctgtc
     1621 catgacggcc ttgatgtcgg cggtcacttc cccgagttcc ctcttgtaga tgtccgcctc
     1681 gagcctgacg atcttctgcc tgttcttgcg ctggatctcc accgggccag acccgtccgt
     1741 gatcgtggcc acgttcgcga gcctcacgac cctcccgtcg gaggtgaaca ggggcgcgtt
     1801 catgagctca tccgggtcat tcttgttctc gaccgtgtag cgggtgtaga tgtcgtacct
     1861 ctcgcccgtg tcccagaacg acgtcgccgc ctggccgtgg tagtaattcc tcagcatggc
     1921 ggctacctgg cccgcggtga gccccagggt ggaggccttg gtgcggtcga tccgcacctg
     1981 gagctctggc ctgtcgttct tctggctcag cgtgaggtcg atcacgcccg ggatggatct
     2041 cgtctcttcg ccgagccgtg aggcatacgc gaggcactcc ttgaggtcgt cgccctggat
     2101 ctccaccacg atgggtttct ggccgcccat gatgaccgag gtcatggggt cctgcgccag
     2161 gatcttgatg ctcgagatgc cggggatccc ggtgatcgag cgcctcagct cgttcgcgat
     2221 ctccttggcg ctccgggagc gctcctgcct gtccacgagc ttgatgaaga cctggcccgc
     2281 gttgggcgcc tcctcgaacc ccaccgcctc gccgaacccc tcctttgtct ggccgcagaa
     2341 ggagaagacg tgcaggagct ccccaggcct gatcgttttg cccacgatcg cgtggatgcc
     2401 ctcgatgacc ttcttcgtct cctcgatccg ggtgccttcg ggcatgcgga cgcctatcgt
     2461 gatgtcgccg gtgtccatct ggggcacgaa ggacgtggcc aggaacggga cgagcgcgag
     2521 gccgcttccg aagaccacga cggccgtgac cacgacggcc ctcgtgtggg ccagggccca
     2581 ggccagaagc ctgccgtagg cctgctcgag cgcctcgaac caccgctcgc tcgcccggta
     2641 ggcgaccccg gcgagccccc tgttcccggg cggcgcggcc tcgcccccgg agaggaggcg
     2701 cgacgccagc atgggggtca tggtgagcga ggagaagagg gaggcgagga tggtcaccac
     2761 catggtgatc gcgaagacct tgaagaagac ccctgtgagg ccggacacga agatgagcgg
     2821 gatgaacacc accgccacgg tgaaggtcga cgcggtcacg gccatgccca tctcgcccgc
     2881 gccgaagatg gacccaaggg ccggcttcgc ccccctttcc aggtgtctca cgatgttctc
     2941 caggatgacg atggcgtcgt ccaccaccat gcccacggcg atggccatgg acatgagcag
     3001 caccatgttc accgtgtagc cgaacaggta gagcacgatg aaggcggcta tcagggaaaa
     3061 ggggatggcc gtgcagatga tgaacgaggt gcgtaccctg cggaggaagg ccacggtcac
     3121 gacggcgacg atgacgatgg cctcgatgag cgtcttcctg aggttggcga tgctgttgag
     3181 gatggtcgca gacgagtcga cgtttatgtt caccctcacg tccgagggga ggttctgccg
     3241 gatggagtcg agcttcttct tgaccgccct gaccacctcc acggtgttcg cgccggtctg
     3301 cttctggagg accatggcga tggcccgctt cccgtttccc cagccgtaca tggtctcgtc
     3361 ctcgtaggaa tccccgaccg tggcgacgtc cctgaggcgc acgggccttc ccccgtacga
     3421 gcccacgatc gtctccccta tctcggccgg cgaggtgtag cggccgggca ccctcaggaa
     3481 gtactccttg ccccccgcct tgatgctccc cgccgggatg ttgaggttct gcgccgagag
     3541 gacctggttg acctgctgga ccgagaggtt gtacccctcg agccgctcgg ggtcgaagtc
     3601 gacgttgatc ctcctgacga gaccgcccac gagcatgacc gcccccacgc ccgggacccg
     3661 cttgagctcg tcggcgatcc ggttgtccac gatgtgatgg agcctgggcc agctcttctc
     3721 ccccgtgacg gtcatcatga ggatgggcgc ggtggcgctg ctgaacttga agagcatggg
     3781 cgggtcgatg tctgcgggca ggtccttctt ggcgaactcg aggcggtcgc ggatgtcgtt
     3841 gctcgccacg tcgaggtcgc tctcccactc gaacttgcac gcgatgaggg atatgttgtc
     3901 catggacttc gaggtgatgc tgtccaggtt ggcgacgccg ttgagctggt tctcgatgac
     3961 atcggtcacc tcggtctcca cgtccgatgc gttcgccccg ggccacgtgg tgatgaccgt
     4021 gatcaccggg ggctccacgt ccgggaacat gtccgtgctc atgtacagga ggctcacggt
     4081 acccatgagg atcacggcgc agaagaccat gagtgtcgcc acgggctgtc tcacggagaa
     4141 ttcggggagc ttcatctact gcactccggg ttcccggggc gcgggccgct gcccggtgac
     4201 gaccaccctc tcgccgggtg cgaggcccga cgccacgagg acaacgtcgc cctgtgaagg
     4261 gccggtctct atgttcttca tgaccgcctt cccgtcctcg accacgtaga cgtagggcgc
     4321 ccccgtgccc ggcagcctct ggaccgcatc cttctccaca acgaccccgg agacgacgcc
     4381 gagcgagatc ctcacgtggg cgaacatgcc cgccttcagc cccttcttgg cgttgtcgac
     4441 gtggatctcg atgtcggcgc tcctgccgga cggcgagagc gtggggttca ccagggcgat
     4501 ctcgccctcg aagacggcgc ccggcacggc gtcgcttgtg acctcgcagc gcatcccctt
     4561 ggctatggac aggtagtcct tctccgtcac ggaggccacg atcttgagcc tggactcgtc
     4621 ggagacgcgc acgatgggtg cgcccggcgc ggagagcgcg ccggggtcga cgtacctggc
     4681 ggatatcacc ccgcctatgg gcgagcgcac cgtgtgctcg gccatgagga tgcggagctg
     4741 gtccagcacc gccttcgagc ggtcgagctg ggcctcggcg gactcgagct gcgcctgcac
     4801 gtggtcgagc ctctgcctcg caacggcctt ctcggcatac aggttctcga tgcgcgcgta
     4861 atccttccgg gtgaggtcga ggttggtccc ggcgagcctg aaggcggcct cggcctccct
     4921 cacctgcgcc tctatggaac gcatctcgag cagggcgatg gtgtcgccgc tacggaccgt
     4981 gtccccccgg tcgaagaaga tcctctcgat gatcttcccg ggcaccttgg ggaacacgtc
     5041 cacctcccgc cacggcctga cgtcccccgt gagcctcagg acccgctcca cgttcccgat
     5101 cgcggccggg accgtgctga caggcacctt ctcggaggcg ggcgcctcgt cgcggtcgct
     5161 tacccctgaa tggaccctcc aggcgaaaag gcccgcgcag gcagccgcga ggatcacaag
     5221 gaggaggacg gctttcctgg gtcttggtcc ggactgtgtc tgcatggcgt cacgaccccc
     5281 cgttcgtgtt cggttgcggc cccgacaatg tgacccccga agccctctcg agcctggcga
     5341 tcgcggacag gtagccgtag agcgcaccgt aatagttcat ctgcgcctgg gagaggtacg
     5401 tccgcgcgtc gagcacctcg gtcgacgagg tgatctgctg ccggtactgg aggttcgtga
     5461 tcctgtagtt ctcgcgggcc tgctcgaggg cctcccgggc gctcgcgat
//
LOCUS       DAOSCR010000096         5508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9331_length_5508_cov_3.726756, whole genome shotgun sequence.
ACCESSION   DAOSCR010000096 DAOSCR010000000
VERSION     DAOSCR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_5508_cov_3.726756"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..496)
                     /gene="fliS"
                     /locus_tag="PLT69_03755"
     CDS             complement(125..496)
                     /gene="fliS"
                     /locus_tag="PLT69_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOJ13590.1"
                     /translation="MNMQGYQAYRKTQIQTADQGGLILLCYDGAIGFLRKALKAISEK
                     DQMSATELLTKAQNVLWELTNGLNRDAGQIAHNLEAIYNYMIRRLVDAQYHSSPAPVN
                     EVLGYLQELRDTWQQVIQKQS"
     gene            complement(692..1867)
                     /locus_tag="PLT69_03760"
     CDS             complement(692..1867)
                     /locus_tag="PLT69_03760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ13591.1"
                     /translation="MKISRLVSNIPGSETLALTAKVNELRAQGKNVIGFTAGEPDFDT
                     PETIKEAAIDSLRRGNTKYTPVGGTMKLKEAVCEAFERDYGFAVSPENVIVSCGAKHS
                     LFNLFFTLFEEGDEVICPAPYWVSYPPMIEMTGAKPVIVDTREAGMKLEAGMVARAIT
                     PRTRGIVINSPSNPTGMVFDRKELEEIAELCLRHDITIISDDIYRKIVFEPNEFFSVA
                     SISPEVAKKTFIIDGSSKTYAMTGWRIGFCIGDKDVIKAMERLQSQSTSNPVSFAQEG
                     ALASLVSDETAAIVEKRRKIFQQRRDLIVRKIREIPGMGIIEPGGSFYAFPSIDGYLD
                     RFKDANAFTQYLIEHALVAVVPGVAFGAPDHVRFSFATSEENIEKGLDRVKEALASL"
     gene            complement(1892..2398)
                     /gene="coaD"
                     /locus_tag="PLT69_03765"
     CDS             complement(1892..2398)
                     /gene="coaD"
                     /locus_tag="PLT69_03765"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOJ13592.1"
                     /translation="MHRDKIAVCPGSFDPITNGHIDIVHRALCVFDKVIIAVGHNPLK
                     TDLFTTQEKLDMIRQSIDSDERIVVDAFEGLLVDYIKKTGACAILRGLRVISDFELEM
                     QLALMNRKLYAPAETFFLMTDYKHLFVSSTIVKATAAAGGSVEGLVPRPVLMKLKEKF
                     PHMKIDPV"
     gene            complement(2403..2927)
                     /gene="rsmD"
                     /locus_tag="PLT69_03770"
     CDS             complement(2403..2927)
                     /gene="rsmD"
                     /locus_tag="PLT69_03770"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOJ13593.1"
                     /translation="MSLRITAGSFRSMRLQSPGQGVRPTSDKVRQAVFSTILAYVQGA
                     HVLDLFAGSGALGFEALSRGAASATFVDASKRCIDVIKKNAAALGVLERVSIVKAEAF
                     SFVGSCSSSYGLIFVDPPYHKSFASDIARDVYKLLDIGGILVIEHESSATIPLPAFKQ
                     KRYGGTSITYVLKE"
     gene            complement(3013..3738)
                     /gene="pyrF"
                     /locus_tag="PLT69_03775"
     CDS             complement(3013..3738)
                     /gene="pyrF"
                     /locus_tag="PLT69_03775"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOJ13594.1"
                     /translation="MAAHDRIIFALDVGDMDSARRWVETLRGHVGWFKVGLELFTAVG
                     PEIVRLLKDSGIRCFVDLKLHDIPNTVSGAVRSLARLGADMATLHISGGKAMLMAALD
                     AAQSEAEAGGLRRPKLVGVSVLTSLGRDDLMETGIPREPHEQADLLARLAASCGLDGI
                     VCSASDLPAIRPSLPPSFLVVTPGIRPSWSESGDQKRISSPSGAIKAGADLLVVGRPI
                     SGAGDPVEAARRIASEIEAALDE"
     gene            complement(3717..4313)
                     /gene="gmk"
                     /locus_tag="PLT69_03780"
     CDS             complement(3717..4313)
                     /gene="gmk"
                     /locus_tag="PLT69_03780"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOJ13595.1"
                     /translation="MRVFVSGPSGVGKTTVIKEVLRKNPDLVLSVSYTTRAPRKDEQD
                     GVDYFFVSRDTFEDMVRKGAFLEWANVHGNLYGTSIEWVEEMESRGFHVLFDIDVQGV
                     KQAKEKGLQGCFILIVPPTIDELERRLHSRGTEDRDSLVRRMENARAELAMWNLYDYL
                     VVNDSLLECIETVHAVILAQRASKSEMIGRLPWLHTIA"
     gene            complement(4310..4600)
                     /locus_tag="PLT69_03785"
     CDS             complement(4310..4600)
                     /locus_tag="PLT69_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOJ13596.1"
                     /translation="MGRNDTRLLNIGFNNGVVASRIIAIVNPSSSPMKRLREEAKERG
                     QLIDATQGRKTRSIIITDSSHVILSAIQAETISNRLSDGGNHKETQASKEDR"
     gene            complement(4643..>5508)
                     /locus_tag="PLT69_03790"
     CDS             complement(4643..>5508)
                     /locus_tag="PLT69_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOJ13597.1"
                     /translation="MPSSMTGFGQAEVSGFHVEIKGVNHRFKDIRVKVPKDMGALEAA
                     IREKVQNSVHRGKVEIVVTALQPEPGLAGTLIDWHLANKAHEQLKAMAETFGGEVTFR
                     DVLAVPGVMVEGRPPSQEDVQPVLEALEAALEDFLSSRAREGEHLCVDIRARIAAIRG
                     MTLSMKDLATTMTSVYKERLHANLSALLAERGTVLDATRLEQEVALLAEKSDITEELV
                     RLEGHIRLLEDTVSQSEGPIGRQADFILQEINREVNTIGSKSQLAQLSRVVIDAKTEI
                     EKIREQVQNIE"
BASE COUNT          936 a         1715 c         1670 g         1187 t
ORIGIN      
        1 ccttcctcga gccgcctctg gatggtctcg tgctccctga cgaggctcga gagggtatcg
       61 aggtcgcctg ctgcgatcgc cccctcctgc ttctcgatga gggatcccca ggactcgaga
      121 aggctcagga ctgtttctgt atgacctgct gccatgtgtc gcggagctcc tggaggtagc
      181 cgagtacctc gtttacgggc gccggggacg agtggtactg tgcatccacg aggcgcctga
      241 tcatgtaatt gtagatggct tccaggttgt gggctatctg gcctgcgtcc cggttgagcc
      301 cgttggtgag ctcccacagg acgttctgtg cctttgtgag gagttcggtc gcactcatct
      361 gatccttctc cgagatggcc ttcagggcct tgcgaaggaa tcctatggcg ccgtcgtaac
      421 acaggaggat cagcccgccc tggtcggcgg tctggatctg ggtcttcctg tacgcctggt
      481 agccttgcat gttcatggag aaccccccga tgcgatgcta ttctctctat cggtgacagg
      541 cttattttct tgagagaaaa aaggaagccg ccgaatgaaa cggccccccg gctgtttcat
      601 gagaaggggg atgcacccga cgcaaagatg ccagggtctc cttggtgcgg tcttggcacg
      661 cggctgtctg attcgacggc cacgtggacc gtcacaggga tgccagggcc tccttgacgc
      721 ggtcgagccc tttctcgatg ttctcctcgc tggtcgcgaa cgagaacctc acgtggtcgg
      781 gcgcaccgaa ggcgacgccc ggcacgacag cgaccagcgc atgctcgatg aggtactggg
      841 tgaaggcgtt tgcgtccttg aagcggtcca ggtagccgtc gatggacggg aaggcgtaga
      901 acgatccgcc cggttcgatg atgcccatcc ccgggatctc cctgatcttc ctgacgatga
      961 gatcccttcg ttgctggaag atcttcctcc gtttctccac gatggcggcg gtctcgtcgc
     1021 tgacgaggga ggcaagcgcc ccttcctggg cgaagctgac ggggttggac gtgctctggc
     1081 tctggaggcg ttccatggcc ttgatcacgt ctttgtcgcc gatgcagaac ccgatgcgcc
     1141 agccggtcat ggcgtaggtc ttggaggatc cgtcgatgat gaaggtcttc ttggcgacct
     1201 caggcgagat gctcgcgacg ctgaagaact cgttcggctc gaacacgatc tttcggtaga
     1261 tatcgtccga gatgatcgtg atatcgtgcc tcaggcagag ctcggctatc tcctcgagct
     1321 cttttctgtc gaacaccatg cccgtggggt tggacggcga gttgatgacg atgcccctcg
     1381 tcctgggggt gatggcccgg gcgaccatgc cggcctcgag cttcatgccg gcctcccggg
     1441 tgtccacgat cacgggcttg gccccagtca tctcgatcat cggcggatac gagacccagt
     1501 agggcgcagg gcatatcacc tcgtcgccct cttcgaagag ggtgaagaag aggttgaaga
     1561 ggctgtgctt ggcaccgcag gagacgatca cgttctcggg cgacacggca aagccgtagt
     1621 ccctctcgaa cgcctcgcag accgcctcct tgagcttcat ggtcccgcct acgggggtat
     1681 acttggtatt gccgcgtctc agagaatcga tggccgcctc tttgatcgtc tccggagtgt
     1741 cgaaatcggg ctcgcctgcc gtgaagccta tgacattctt gccttgcgcc ctgagctcgt
     1801 tgaccttggc ggtcagagcc agcgtctcgg aaccggggat gttggagaca aggcgggata
     1861 tcttcatata ctgtcctttc tggcagatat tctacacagg gtcgatcttc atgtgcggaa
     1921 acttttcttt cagcttcatg agcaccgggc gcggcacgag cccctccacg gaccctccgg
     1981 cggcagcggt ggccttcacg atggtcgagc tcacgaagag gtgcttgtag tccgtcatga
     2041 ggaagaacgt ctccgccggc gcgtagagct tcctgttcat gagggcgagc tgcatctcga
     2101 gctcgaagtc cgagatcacc ctcagccccc tgaggatggc gcaggcgcct gtcttcttga
     2161 tgtagtcgac gagcaggccc tcgaacgcat ccacgacgat cctctcgtcg ctgtctatgg
     2221 actggcggat catgtcgagc ttttcctgcg tggtgaacag gtccgtcttc agaggattgt
     2281 gcccgaccgc tatgatcacc ttgtcgaaga cgcacagcgc cctgtggacg atgtctatgt
     2341 ggccgttggt gatggggtcg aacgatccgg ggcagacggc gatcttgtct cggtgcatgc
     2401 ggtcattcct tcaggacgta ggtgatcgag gttccgccgt agcgtttctg cttgaaggcc
     2461 ggaagtggga tcgtcgcgga gctctcgtgt tcaatgacca gtatcccccc aatatcgagc
     2521 agcttatata catcacgagc gatatccgat gcaaacgatt tgtggtacgg cgggtccacg
     2581 aagatgaggc cgtacgatga actgcacgag cccacgaacg aaaaggcctc cgccttgacg
     2641 atgctcaccc tctcgagtac gcccagggct gcggcgttct tcttgatcac gtcgatgcac
     2701 cttttcgacg cgtcgacgaa cgtggccgat gcggcccccc tgctcaacgc ctcgaagccg
     2761 agcgccccgc tcccggcgaa cagatcgagc acgtgagcgc cttgcacgta tgcgaggatc
     2821 gtcgagaaga ccgcctgcct gaccttgtcg gaggtcggcc gcacgccctg gccggggctc
     2881 tgcagcctca tggagcggaa actgcctgca gtgatcctga ggctcatggg atcgtcctcg
     2941 gcggtgtccg gccgtcggtc atgggtggga agaaggatgc atggaagggc atcgaaaccg
     3001 ggtgcaggcc cctcactcgt cgagcgcggc ctcgatctcg gaagcgatcc tccgagccgc
     3061 ctccaccggg tcgcccgcgc cgctgatggg ccttcccacg acgaggagat cggcgcccgc
     3121 cttgatcgcg ccggagggag aggagatgcg tttctggtct ccgctctcag accacgaagg
     3181 cctgatcccg ggggtcacga ccaggaagga aggggggagg ctcggcctga tggccggcag
     3241 atcggatgcc gagcacacga tgccgtcaag accgcaggag gctgcaagcc gcgcgagcag
     3301 gtcggcctgc tcgtggggct cccgagggat gccggtctcc atgaggtcat cccttcccag
     3361 cgaggtgagc acgctcaccc cgacgagctt cggccgtctc aggcccccgg cttccgcctc
     3421 gctttgtgca gcatcgagcg ccgccatgag catcgccttg ccgcccgaga tgtggagggt
     3481 cgccatgtcc gcgccgagcc tcgcaagcga cctcactgcg cccgagacgg tgttcgggat
     3541 gtcgtgcagc ttgagatcga cgaaacacct gatcccgctg tccttgagaa gcctcacgat
     3601 ctcgggaccc accgccgtga agagctccag gcccaccttg aaccacccga cgtgtcccct
     3661 gagcgtctcc acccacctcc tggcggagtc catgtcgccg acgtcgaggg cgaagattat
     3721 gcgatcgtgt gcagccacgg cagccttccg atcatttcgc ttttgctcgc acgctgtgcg
     3781 aggatgacgg catgcacggt ctcgatgcac tccaggagtg aatcgttcac cacgaggtaa
     3841 tcgtagaggt tccacatggc gagctcggcc ctcgcgttct ccatgcgtcg cacgagagag
     3901 tccctgtcct cggtacccct cgaatggagg cgtctttcca gctcatcgat ggtcgggggg
     3961 acgatgagga tgaaacagcc ttgcaacccc ttctccttgg cctgcttcac cccctgcaca
     4021 tcgatgtcga acaggacgtg gaatccccgg ctttccatct cctcgaccca ctcgatggac
     4081 gttccgtaga ggtttccatg cacgttcgcc cactcgagga aggccccctt cctgaccatg
     4141 tcctcgaagg tgtctctcga cacgaagaaa tagtccaccc cgtcctgttc gtccttgcgc
     4201 ggcgccctcg tcgtgtagga gacggacagt accaggtccg ggttcttcct gagcacctcc
     4261 ttgatgacgg tggtcttgcc gaccccggag gggccggaga cgaacaccct cacctgtcct
     4321 ccttggaagc ctgggtctcc ttgtggttgc ccccgtcgga gaggcggttg gagatggtct
     4381 cagcctgtat ggcgctcagg atgacgtgag agctgtccgt gatgatgatc gatctcgtct
     4441 ttctcccctg ggtggcgtcg atgagctgtc cgcgttcctt ggcctcttcg cgcaggcgct
     4501 tcatgggtga agaagatgga ttgacgatgg cgatgatcct cgaagcgacg accccgttgt
     4561 tgaagccgat attgagcagc ctcgtgtcgt tcctgcccat gccctctctc ctttctcatt
     4621 ccctcgaaca catcgaagaa cgctattcta tgttttgaac ctgctccctg atcttttcta
     4681 tctctgtctt cgcgtcgatc acgacacggc tcaactgagc gagctggctc ttggagccga
     4741 tcgtgttgac ctcccggttt atctcctgga ggatgaaatc agcttgcctg cctatggggc
     4801 cttcgctctg cgatacggtg tcctccagga ggcggatgtg gccttcgagt ctcaccagtt
     4861 cctcggtgat gtcgctcttt tcagccagca atgcgacctc ctgttccagg cgggtggcat
     4921 ccagaaccgt ccccctctcc gccaggaggg cgctcaaatt ggcatgtagc ctttctttat
     4981 atacacttgt catggtggtt gcaaggtctt tcatggacag cgtcatgccc ctgatggcgg
     5041 cgatcctcgc cctgatgtcg acgcacaggt gctcgccctc tcgggccctg gaggacagga
     5101 agtcctcgag cgccgcttcc agggcctcga ggaccggctg gacgtcctcc tgggacgggg
     5161 gccggccttc gaccatcaca ccaggaacgg cgagcacgtc cctgaaggtg acctcgccgc
     5221 cgaaggtctc ggccatcgcc ttcagctgct cgtgcgcctt gttcgcgagg tgccagtcaa
     5281 tgagggtccc ggccaggccg ggctccggct ggagggccgt gaccacgatc tccaccttgc
     5341 ccctgtgaac cgagttctgg accttctccc tgatcgcagc ctccagggcc cccatgtcct
     5401 tgggcacctt cacgcggata tccttgaacc tgtgattcac gcccttgatc tcgacatgga
     5461 accccgagac ctcggcctgt ccgaacccgg tcatgctcga cggcatca
//
LOCUS       DAOSCR010000097         5498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9358_length_5498_cov_3.840162, whole genome shotgun sequence.
ACCESSION   DAOSCR010000097 DAOSCR010000000
VERSION     DAOSCR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_5498_cov_3.840162"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1385)
                     /locus_tag="PLT69_03795"
     CDS             complement(51..1385)
                     /locus_tag="PLT69_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOJ13598.1"
                     /translation="MRRILIANRGEVVLRILRTARELGLETIVVYSEADRDMEYLGLA
                     DEAVKIGPEQASKSYLNMDAIISAALTWKAHAVHPGYGFLSENPQFASLCEEQGIQFI
                     GPSSATLRTIGNKSRTKEVARSLGIPTIPGSQGMVGDIGHGLALARELGYPVILKSLY
                     GGGGRGMKVASNEEQLKSQFAGVSSEASAAFGRSEIYIEHYISSPRHLEVQVLADNHG
                     NIRVYGDRECSIQRRHQKIIEEAPIHNIDEGLRKNLWEWSVQIVRAIGFTGIGTVEFL
                     MDKQERVYFLEINGRLQVEHPVTEVVTGKDLVRDQIMVAAGAVLDWEHVKQEGHAVEC
                     RVNAENPSKNFMPTTGTVTRLRLPGGPGTRIDTHLFQGCVVTPFYDPLLVKLVAHDTD
                     RKGALKRMTRMIDETVIEGITTNLPLLRKLITHEQFQEGNGDTSLVHRVVGR"
     gene            1537..2601
                     /locus_tag="PLT69_03800"
     CDS             1537..2601
                     /locus_tag="PLT69_03800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOJ13599.1"
                     /translation="MARTLAAALFIIASILASCAHTQGGPKASVEPKAGCGQAAVGPP
                     APAGTGGGEPSKAGDGPQAPGPDAQGSASPQGLASHLREKVEAVLVEGGLEEGQARAL
                     AYDPRLVLDEAFILRNLIIAPPKASPKTPGVMAYDQAYISKGCTFMSENEEFLEALRE
                     RYGTSPEVLTAVLIVESRLGTYPMRYKVASVLASMALAKDPAFILELKERNPGLGEAL
                     VDADSLDKAVLKARWAARELPELVTLARELDMDPIEIDGSVSGALGPAQFIPSTFRKY
                     GVDGDGDGKRNPFAMKDAIASMANYIRLAGWREDADEGRMRGALWHYNHSEVYVNTVM
                     QIYRELSRCRTDDVGAAAAK"
     gene            3088..3966
                     /locus_tag="PLT69_03805"
     CDS             3088..3966
                     /locus_tag="PLT69_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ13600.1"
                     /translation="MEIASLLAAWKAEFDTFLEDYINRAMAGPGLSQACAYSLKAGGK
                     RLRPVLAMMAAEAAGGRRRATLDAGLAIEMIHTFSLIHDDLPAIDDDDTRRGVPTCHR
                     AFGEATAILAGDALIFQAFSVICTSAYPLEVKIDLCSAMAEACGADGLVKGEYEDVAA
                     EGANPGIESIEGIYERKTAALFGLSMYAGSRVATDERDVVACLRRYGKHLGMAFQAMD
                     DILDATSDAETLGKASRKDASKGKATLVRVMGVDASRAWAERQTALAVEALDGLGKTR
                     TEALRELARWQLERVR"
     gene            3969..>5498
                     /gene="dxs"
                     /locus_tag="PLT69_03810"
     CDS             3969..>5498
                     /gene="dxs"
                     /locus_tag="PLT69_03810"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOJ13601.1"
                     /translation="MAILETVHSPDDLKDLGIAELELLASEIRGLIIETVSSTGGHLA
                     SSLGAVELAVALHHVFNTPRDKIVWDVGHQAYAHKIITGRRGAFPTLRQKGGVSPFIN
                     PFESPYDAFVSGHTGNAVPAASGICETMEKAGASCKVVVVIGDGSLSNGLTFEGLNFA
                     GMRKQNMLVVLNDNTMFISKRVGALADYLSRIMTSRRAKDVREGIKHALKGVPLVGNA
                     LYRAAKILEGNIKGVVTEGLLFEEMGFRYVGPIDGHNMAHLVEALKNVSAWNEPILLH
                     VITQKGHGYSPAMKDPEHFHGVGRFDLENGESASVHAPTQETYSDVFGRSIMELAERD
                     PRIVALSAAMTTGTGLKAFSKRYPERYYDVGIAEGHAVTMAAAMALYGLRPVVAIYST
                     FLQRAYDSIVVDVALQRAPVLFAVDRAGLVGPDGPTHHGAFDLAYLRSVPNLTLMVPR
                     DQLMLRLMLETGLSLNAPAALRYPRESVVERPISGPKLEHGRCQVLRPGSRAVVFCAG
                     PL"
BASE COUNT          961 a         1838 c         1818 g          881 t
ORIGIN      
        1 ccggccggat gcgaggcccc ccttctctct gcgccggtga cggggtgagg tcacctgccc
       61 acgacccggt gcacgagcga ggtgtcgccg ttcccttcct ggaactgctc gtgggtgatg
      121 agtttcctga gcagggggag gttcgtggtg atgccttcta tgaccgtctc gtcgatcatg
      181 cgggtcatgc gcttgagcgc ccctttccgg tcggtgtcgt gggcgacgag cttcaccagg
      241 aggggatcgt agaacggggt gaccacgcac ccctggaaga ggtgggtatc gatcctggtc
      301 ccggggccgc cggggagcct gagcctcgtg acggtccccg tggtgggcat gaagttcttg
      361 gacgggttct cggcgttgac ccggcactcc actgcatgtc cctcctgctt gacatgctcc
      421 caatccaaga cggcgccggc cgccaccatg atctggtccc tgacgaggtc cttgccggtg
      481 acgacctcgg tcacgggatg ctccacctgg agccggccgt tgatctcgag gaaatagacc
      541 cgttcctgct tgtccatgag aaactccacg gtgccgatgc ccgtgaaccc tatggccctg
      601 acgatctgca cggaccactc ccagaggttt ttcctcagcc cctcgtcgat gttgtggatg
      661 ggggcctctt cgatgatctt ctggtggcgc ctctggatgg agcactcgcg gtcgccgtag
      721 acgcggatgt tcccgtggtt gtcggcgagc acctgcacct ccaggtgcct cggcgaggag
      781 atgtaatgct cgatgtagat ctcgctcctg ccgaaggcgg cgctcgcctc gctgctgacg
      841 ccggcgaact ggctcttcag ctgctcctcg ttggaggcca ccttcatgcc cctgccgccg
      901 cctccgtaga gggatttcag gatcaccggg taacccagct cccttgccag cgccaagccg
      961 tgcccgatgt cccccaccat cccctgcgaa ccgggtatcg tggggatgcc gagggagcgc
     1021 gcgacctcct tggtgcggga cttgttgccg atggtcctca aggtggcgct cgagggaccg
     1081 atgaactgta tgccctgttc ctcgcagagg gacgcgaact gcgggttctc ggagaggaac
     1141 ccgtagccgg ggtggacggc atgggccttc cacgtgagcg ccgcggagat gatggcgtcc
     1201 atgttgaggt agctcttgga tgcctgttcg ggaccgatct tgaccgcctc gtccgcgagc
     1261 ccgaggtatt ccatgtccct gtccgcctcg gagtagacca cgatggtctc gaggccgagt
     1321 tccctcgcgg tcctcaggat cctcaggacc acctcgcccc tgttggctat gagaatccgc
     1381 ctcatggcgt ggttgtatga ctccgccttc ggcaagtcaa gcagggcttg cacccgccgg
     1441 ggcaccccgg cgccacggtc tcgccgcgac ggccgggccc cctgcacaag gcccttgtgc
     1501 ccgggtgaca aacggtatac acgcatcgcc atgaccatgg cgcgaacact ggcggccgca
     1561 ctgttcatca ttgccagcat cctcgcatcc tgcgcgcaca cgcagggcgg tccgaaagca
     1621 tccgttgagc ccaaggcggg ctgcggacag gcggctgtcg ggccccctgc accggcgggg
     1681 acgggcggcg gcgaaccctc gaaggcaggg gacgggccgc aggccccggg accggacgca
     1741 caagggtccg cctcgcccca aggccttgca tcccacctcc gggagaaggt cgaggccgtt
     1801 ctcgtcgagg gcggcctgga ggaggggcag gcacgggccc tcgcgtacga ccccaggctg
     1861 gtgctcgacg aggcgttcat tctcaggaac ctgatcatcg ccccgcccaa ggcctcgccg
     1921 aagacccctg gcgtcatggc gtacgaccag gcgtacatct ccaaggggtg cacgttcatg
     1981 tccgagaacg aggagttcct ggaagccttg cgagagcggt acgggacgtc accggaggtg
     2041 ctcaccgcgg tcctcatcgt ggaaagcagg ctcggcacgt atcccatgcg ctacaaggtg
     2101 gcctccgtgc tcgccagcat ggcgctcgcg aaggacccgg ccttcatcct ggaactcaag
     2161 gagcgcaacc ccgggctcgg cgaggccctc gtcgacgccg attccctcga caaggccgtc
     2221 ttgaaggcca ggtgggccgc tcgggaacta cccgagctcg tcacgctcgc aagggagctg
     2281 gacatggacc ccatcgagat cgacggctcg gtgtccggcg cactcggacc ggcgcagttc
     2341 attccttcca cgttcaggaa gtacggggtc gacggcgacg gcgacgggaa gcgaaacccc
     2401 ttcgcgatga aggacgcgat cgcgagcatg gcgaactaca tcaggctcgc gggctggcgc
     2461 gaggacgcgg acgagggcag gatgaggggg gcgctgtggc actacaacca cagcgaggtc
     2521 tacgtgaaca cggtcatgca gatctaccgc gagctctccc gatgccggac cgacgacgtc
     2581 ggcgcggctg ccgcgaagtg agcagcaaag agcgccttcg gcctcaggcg agcgggccgt
     2641 gcacgaaacg ttcctccgag cgcccggccg cgggcgaagc tcgcctgccc cgagacttct
     2701 tctaggaccc gtggcccgaa cagaactcag gcggcgccgg gagacgaaag atcaagaagc
     2761 caaccggcct cgacaccccg tcctgcacca agccgcaccg tacgccatgg acaagacggt
     2821 gcgcgacctc cggggtcacg gcggtttcta gaacgtctgc acctggcggc gcccccccga
     2881 gtccatggga cctcagcctt ctccgcaggg tctataccca tgccgcggcc ttgcgggtat
     2941 accccaaggc ccgcatcacc cggggcgcac gcggatgcgc ggcaccccgg gcggccagaa
     3001 gaggggccgg cacgacgagg cgctcccgat ccaggatgtt gaagattgca cacgtgcgtg
     3061 ctattgcctg gaaagaggtg ccaacgcatg gaaatcgcat cgctgcttgc cgcatggaag
     3121 gccgagttcg acacgttcct cgaagactac atcaaccggg ccatggccgg ccccgggctg
     3181 tcccaggcat gcgcatacag tctcaaggcc ggcggcaaga ggctcaggcc ggtcctcgcc
     3241 atgatggccg cggaggcggc cgggggtcgg cggcgtgcaa cccttgatgc cggcctcgca
     3301 atcgagatga tccacacctt cagcctcatc cacgacgacc tgcccgccat cgacgacgac
     3361 gacacgagac gcggcgtgcc cacgtgccac agggcgttcg gggaggcgac cgccatcctg
     3421 gccggcgacg cgctcatctt ccaggcgttc tcggtgatct gcacctctgc ctaccctctc
     3481 gaggtcaaga tcgacctctg ctccgccatg gccgaggcat gcggcgccga cggcctcgtc
     3541 aagggcgagt acgaggacgt ggccgccgag ggcgcaaacc cagggataga gtccatcgag
     3601 ggcatctacg agaggaagac cgctgccctg ttcgggctca gcatgtacgc cggatcccgg
     3661 gtcgcgaccg acgagaggga cgtggtcgcc tgcctgcgcc gctacgggaa acacctgggc
     3721 atggccttcc aggcgatgga cgacatcctt gacgccacgt ccgacgccga gaccttgggc
     3781 aaggcctcga gaaaggacgc gtccaagggc aaggccaccc ttgtccgcgt catgggggtc
     3841 gatgcctccc gggcatgggc cgaacgccag accgccctgg ccgtggaggc gctggacggc
     3901 ctcgggaaga cccggacgga ggccctcagg gagctcgcgc gctggcagct cgaaagggtg
     3961 aggtagtcat ggcaatcctg gagacggttc actcccccga cgacctcaag gacctcggca
     4021 tcgctgagct cgagctcttg gccagcgaga tcaggggcct catcatagag accgtgagct
     4081 ccacgggcgg ccacctcgcg tcgagcctgg gcgccgtgga gctcgccgtg gccctgcacc
     4141 acgtgttcaa caccccgagg gacaagatcg tctgggatgt gggccaccag gcgtacgcgc
     4201 acaagatcat cacgggaagg cgcggcgcct ttcccaccct gcggcagaag gggggcgtga
     4261 gccccttcat caaccccttc gagagcccgt acgacgcgtt cgtctccggc cacacgggca
     4321 acgcggtccc ggctgcaagc ggcatctgcg agaccatgga aaaggccggg gcatcgtgca
     4381 aggtggtcgt cgtcatagga gacgggtccc tgtccaacgg cctcaccttc gaggggctca
     4441 acttcgcggg catgaggaag cagaacatgc tcgtcgtcct caacgacaac accatgttca
     4501 tctccaagcg ggtgggcgcg ctggcggact acctgagccg catcatgacc tccaggcggg
     4561 ccaaggacgt gcgcgagggg atcaagcacg ccctcaaggg ggtccccctg gtgggcaacg
     4621 ccctctacag ggccgccaag atcctcgaag gcaatatcaa gggggtcgtg accgagggac
     4681 tcctgttcga ggaaatgggg ttcaggtacg tgggtcccat cgacgggcac aacatggccc
     4741 acctggtaga ggccctcaaa aacgtctcgg catggaacga gccgatcctc ctgcacgtga
     4801 tcacgcagaa aggccacggg tacagccccg ccatgaagga ccccgagcac ttccacggcg
     4861 tcggcaggtt cgacctcgaa aacggcgaga gcgcgtccgt ccacgccccg acccaggaga
     4921 cctactccga cgtgttcggc cgatcgatca tggagcttgc ggaaagagac ccgcgcatcg
     4981 tggccttgag cgccgccatg accacgggca ccgggctcaa ggccttctcg aagaggtacc
     5041 ccgaaaggta ctacgacgtg ggcatagccg agggtcacgc cgtgaccatg gccgcggcca
     5101 tggcgctcta cggcctgcgg cccgtggtgg ccatctactc gacctttctc cagcgcgcct
     5161 acgattccat cgtggtggac gtggccctcc agcgtgcgcc ggtgctcttc gcagtggaca
     5221 gggccgggct ggtggggccc gacggcccca cccaccacgg cgccttcgac ctggcctacc
     5281 tcaggagcgt cccgaacctg acgctcatgg tgcccaggga ccagctcatg ctcaggctca
     5341 tgctggagac ggggctgtct ttgaacgcgc cggcggccct caggtacccg agggaaagcg
     5401 tggtggaaag gccgatttcc ggcccgaaac tcgagcacgg caggtgccag gtcttgaggc
     5461 cgggctcgag ggcggtggtg ttctgcgccg ggccgctg
//
LOCUS       DAOSCR010000098         5460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9433_length_5460_cov_2.985384, whole genome shotgun sequence.
ACCESSION   DAOSCR010000098 DAOSCR010000000
VERSION     DAOSCR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9433_length_5460_cov_2.985384"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1498)
                     /gene="arsB"
                     /locus_tag="PLT69_03815"
     CDS             complement(311..1498)
                     /gene="arsB"
                     /locus_tag="PLT69_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOJ13602.1"
                     /translation="MTDTKGKKLSWLDRFLTLWIFLAMGAGILLGYFSPGVTRFIAGL
                     QVGTTSIPIAIGLILMMYPPLAKVKYEEMGKVFRHKRLLGVSLVQNWVVGPIVMFALA
                     VVFLHDYPEYMIGVILVGLARCIAMVIVWNELAEGDRELAVGLVAFNAVFQVLFYAVY
                     IFVFITVGLNWLGLVSGLDVRISIADAAKTVFIYLGIPFIAGVLTRLTLLRAKGRKWY
                     DEVFVPRISPVTLVALLFTIVVMFSLKGQYIVELPLDVVRVAIPLTVYFFFMWFFTFF
                     IAYRIRANYAQTATVAFTAASNDFELAIAVAVAIFGIHSNQAFATVIGPLLEVPVLIS
                     LVNVSIRLRKKYFPKAVETEDELQVMHAPWAREATGPGILPQGRTCAEGSLRPPASCE
                     GAR"
     gene            complement(1479..1889)
                     /locus_tag="PLT69_03820"
     CDS             complement(1479..1889)
                     /locus_tag="PLT69_03820"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ13603.1"
                     /translation="MQKEAALFKVLSDQTRLRLACLLLFNGETCVCRLAEALNEPDFK
                     ISRHLGVMRSAGMVRARRQGTWIYYRLVKPRSRLAQSLHDFLYEGMVNDRTLKKDLER
                     LSKTSCGEPGRHAKKARRGEHDKGRGAPDDGHEG"
     gene            2296..2481
                     /locus_tag="PLT69_03825"
     CDS             2296..2481
                     /locus_tag="PLT69_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13604.1"
                     /translation="MKFKYHVPLIGFLVPTFVISSLMFAYRGFPPTDQLVGFYVCVAG
                     AVATYYAGIHTVLKDKP"
     gene            complement(3076..3150)
                     /locus_tag="PLT69_03830"
     tRNA            complement(3076..3150)
                     /locus_tag="PLT69_03830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3116..3118),aa:Val,seq:cac)
     gene            complement(3508..>5460)
                     /locus_tag="PLT69_03835"
     CDS             complement(3508..>5460)
                     /locus_tag="PLT69_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13605.1"
                     /translation="SPSAERSMKDHTENVRTVFEKAPFAVCAFDRSGTVVYINKVLES
                     NMDPSTMPFVGKSLYELIHTFLVDDRLEKNLKRLIETNKPFNIVVETLSSPKVKASGF
                     INITGYKVDSLYVLIGDFMSGGVARESRYRTLIEDAPDAILIMNHGFITFANQAFVDI
                     MQVPITEILGRSLFDFVDEQGREALSPLRRKTARKFFTQVTVNTKTGQRVVEGRFQFI
                     EDRPGTSLAMLRDVTEKVLLQKRLMRQNQDLAAINTISKTLSSSVDLVEVLQKTLEQV
                     LQIMNIEMGFIHLLDERKNVLRCVVSHGITDDILESLREIRVGDGIAGRVAAGGKPII
                     IENASMDPRLHSITLRQKGLQSYASIPIQSRTRLMGVMNIGSFGQRRISPEDERLLIT
                     IGVHMGAVIENIELFQEVSRTTEELRDALDLIRQRNEELRQLVSTVSHDLKNPIVAIN
                     GFCERFMKTAANKLSPKELQYVEAIRESGRHMEEFVSNLLTLSAVENLKLKKEEFSTR
                     DVLDDLLKDLSPLIDATQGRVIVERDLPTIKADRLRVTQVFTNLISNALKYAHPDRPP
                     MVEVGHQLQGNQHIFSVKDNGIGIPEEHHRSIFDIFYRASDNHVEGTGIGLSIVKKAL
                     SVMGGDIWVKSQPGKGSTFYFTIPKE"
BASE COUNT          977 a         1698 c         1729 g         1056 t
ORIGIN      
        1 cctagcccaa agccgtctca ggcgccggtg gcccctcgac atcgcacagg cgcgctccgc
       61 ctgcgcagtg ctgcgggccc ggcacacaag acggtcgcgc atcccttggt tgcacagcct
      121 cactcaaccg gtcagtgcga tcacctgagc acccaggact ctgtgctccc ccagcagcaa
      181 tatgggcaag ccaatgagcg atgacctgag cgcccatgga ctctgcgcac gaggcttcca
      241 tgatccctcg acatcgaacg ggcgcgcccc acctggcgga tcgatggcct gagcgcaggc
      301 cttggtttca tcaccgcgct ccctcgcagg aagccggtgg ccgtagagaa ccttcggcgc
      361 aggttctccc ctgcggcagg atgcccggcc cggtcgcctc cctggcccag ggggcgtgca
      421 tcacctgcag ctcgtcttcc gtctcgacgg ccttcgggaa gtatttcttc ctcagcctga
      481 tggacacgtt cacgaggctt atgagcaccg gcacctcgag gagcggcccg atgaccgtgg
      541 cgaaggcctg gttcgagtgg atgccgaaga tggcgaccgc cacggcgatg gcgagctcga
      601 agtcgttgct cgcggccgtg aaggccacgg tggcggtctg ggcgtagttc gcccggatcc
      661 tgtaggcgat gaagaaggtg aaaaaccaca tgaagaagaa gtagacggtc aagggtatgg
      721 ccaccctgac cacgtcgagc ggcagctcga cgatgtactg gcccttgagc gagaacatga
      781 ccacgatggt gaagagcagc gccaccagcg tgaccgggct gatccggggg acgaagacct
      841 cgtcgtacca cttcctgccc tttgccctga gcagcgtcag gcgcgtcagc acccctgcga
      901 tgaaggggat gcccaggtag atgaacacgg tctttgccgc gtccgcgatc gagatcctca
      961 cgtcgaggcc cgagacgaga cccagccagt tgagccccac ggttatgaac acgaagatgt
     1021 acaccgcgta gaagagcacc tggaagaccg cgttgaacgc caccaggccc accgccagct
     1081 cccggtcgcc ctctgccagc tcgttccaca cgatgaccat ggctatgcac cgggccagcc
     1141 ccacgaggat gacgccgatc atgtactcgg ggtagtcgtg gaggaagacg acggcgagcg
     1201 cgaacatgac gatgggcccc acgacccagt tctgcacgag cgagacgccg agcaggcgct
     1261 tgtgcctgaa cacctttccc atctcctcgt acttcacctt cgccagggga gggtacatca
     1321 tgaggatgag cccgatggcg atgggtatcg acgtggtgcc cacctggagc ccggcaatga
     1381 acctggtgac cccgggcgaa aagtacccga ggagaatgcc cgcgcccatg gccaggaaga
     1441 tccacagggt gaggaaccgg tcgagccagg aaagcttctt acccttcgtg tccgtcatcg
     1501 ggtgcccccc ttcccttgtc atgttctccc ctacgcgcct ttttcgcgtg ccggcccggt
     1561 tccccgcacg aggtcttcga aagcctctcc aggtcttttt tgagcgtacg gtcattcacc
     1621 atgccctcgt acaggaagtc gtggagagac tgcgcgagtc tggagcgggg cttcacgagc
     1681 ctgtagtaga tccacgtgcc ctgtctcctc gccctgacca tgccggccga ccgcatgacg
     1741 ccgaggtgac gcgatatctt gaaatccggt tcgttcaggg cctcagcgag cctgcacacg
     1801 caggtctccc cgttgaacag gaggaggcac gcgagcctca gccgtgtctg gtccgagagc
     1861 accttgaaga gggccgcctc cttctgcatc gaatcatccc cggttttgcg attatctgca
     1921 tatttgcact gatttgcaaa tatcaaacag cgttctcacc ggtcaagggc tgtgtttttg
     1981 tcctgcacgc cggtctctct cggcgacccc ggtccagagt atggcgcgtg cggtctcgat
     2041 gtcgacgccc acggcaggca cgtgggagac gccctctcgg cgtttttcgc cctgggcccg
     2101 tctgaacggc gagatctcga gggagaatgg cactcaaacc cacgatccca gggatcgcga
     2161 tggacaaagc ggaagacagg gtgaaatctc cccaccccca tgacaccggt gtctcaacct
     2221 tggctgattt ggcatgcccg cacgcggcct ttataatgga cgtgtacagg tcacctcatg
     2281 caggaggggg tttcgatgaa gttcaagtat cacgtcccgt tgatcggttt cctcgtcccc
     2341 acgttcgtca tatcgagcct catgttcgcc tacagggggt ttccccccac ggaccagctg
     2401 gtcgggttct acgtatgcgt tgcaggcgcc gtcgcgacct actacgcagg cattcacacg
     2461 gtcctgaaag acaagcctta gcgagacgtt cgaggccgac ggccgggatg acctcagctt
     2521 ggcgcggccc gccactccat aggggcacgg ggcggccggc gaccctctgg gcgcggtgaa
     2581 tgcggcgccc gagtgacggg ggcttccgcg gcctcctgac gagggggatc gagcgacccc
     2641 atgtgcacgg tgaacgcggc tgtcaaaggg tcggccgggc ggggcctgca gaagcccact
     2701 cgcgtatgga tcgtcagcca tcctcgctaa cggcacaatc gtaggcatta cgaagccccc
     2761 tcgcgtatgg gtcgtcaagg tccgcggccc tgacatgtga gcgcgcttgg tctcctggca
     2821 ggtgcgtgat cggcggcaca gggagaccgc ccttgtcgct gcggtctccg ggctcctgcc
     2881 gcaggagcct cctgcatggc tgttcaaccg ggcggaaaga cggattgaaa acatgaccag
     2941 cagcgccgtg tcgtggcggt gaaagacgcc tgggcgcacg gcgttgtttt tcgtgtcccc
     3001 agtgaggggg atcggacacc ggtcggaaaa agaccacacc ggtctttcgg gcgtgtcgtg
     3061 ttttgaggag aggggtggtg ggccgcatag gatttgaacc tatgacttct accgtgtgaa
     3121 ggtagcgctc tccccctgag ctagcggccc ttctcgatgc ggacctgtat acaatccggc
     3181 cgccactgtg tcaaggggga tgaacggttg aacaaacccc gcggcagccg gtgagatgct
     3241 cgccacccgg cctcggggcg acatgacgca ttcgcctacc cgggacatgc gggcaagctt
     3301 gtcgaaggag tctcaggtgg agcccaaccg ggacgacagg acgccttctt cgcgcaccca
     3361 gcccctgccc cgttacgccg tccatggccc ccggccgaga tgcgcacctg ccacggtgaa
     3421 ccgagccggg aagaggagca acgcgagcgg ccccggcaac ccccgccgag agtggcgttg
     3481 gtgccgggcg cccatagacg cgccccttca ttccttgggg atggtgaagt agaaggtgga
     3541 ccccttgccc ggctgagact tcacccagat gtcaccgccc atgacgctga gcgccttctt
     3601 cacgatggac agcccgatgc ccgtcccctc cacgtggttg tccgaggccc tgtagaagat
     3661 gtcgaagatg ctcctgtgat gttcttcggg gatccctatg ccgttgtcct tgaccgagaa
     3721 gatgtgctgg ttgccctgga gctggtgccc gacctccacc atgggaggcc tgtcggggtg
     3781 ggcgtacttc agcgcgttgg atatgaggtt ggtgaagacc tgggtcaccc tgagccggtc
     3841 cgccttgatg gtggggaggt cgcgctcgac gatgacacgc ccctgggtcg cgtcgatcag
     3901 gggggagagg tccttgagga ggtcgtccag gacatcgcgc gtggagaact cttccttctt
     3961 gagcttgagg ttttccacgg ccgacagggt gaggaggttc gagacgaact cctccatgtg
     4021 cctgccgctc tcccggatcg cctccacgta ctggagttcc ttgggcgaga gcttgttcgc
     4081 cgcggtcttc atgaagcgct cgcagaaacc gttgatggcc acgatggggt tcttcaggtc
     4141 gtgcgagacg gtcgagacga gctgcctgag ctcctcgttg cgctggcgga tgaggtcgag
     4201 ggcgtccctg agttcctcgg tcgtcctcga gacttcctgg aacagctcta tgttctcgat
     4261 cacggcgccc atgtgcacgc ctatggtgat gagcaggcgt tcgtcctcgg gagagatgcg
     4321 acgctggccg aaggacccga tgttcatgac ccccatgagc ctcgtacggg actggatggg
     4381 tatcgaggcg tacgactgga gacccttctg cctgagcgtg atggaatgga gcctggggtc
     4441 catcgatgcg ttctcgatga tgatcggctt gccgccggcc gcgaccctgc ccgcgatgcc
     4501 gtcgccaacc cgtatctccc tgaggctctc gaggatgtcg tcggtgatcc cgtgggacac
     4561 gacgcacctg agcacgtttt tcctctcgtc cagcaggtgg atgaagccca tctcgatgtt
     4621 catgatctgg aggacctgct cgagggtctt ctggagcacc tccaccaggt ccaccgacga
     4681 ggagagggtc ttcgagatgg tgttgatcgc ggccaggtcc tggttctggc gcatgagcct
     4741 cttctggagg agcaccttct cggtcacgtc cctgagcatg gccagcgatg tgccgggcct
     4801 gtcctcgatg aactggaaac gcccctccac caccctctgg ccggtcttcg tgttcacggt
     4861 gacctgcgtg aagaacttcc tggccgtctt tcgcctgagc ggggacaggg cctccctgcc
     4921 ctgctcgtcc acgaaatcga acagggaacg gcccaggatc tcggtgatcg gaacctgcat
     4981 gatgtcgacg aaggcctggt tcgcgaacgt gatgaacccg tggttcatga tgaggatcgc
     5041 atcgggcgca tcctcgatga gggtccggta ccggctttcc cgcgcgacgc ccccgctcat
     5101 gaagtcgccg atgagcacgt agagggaatc gaccttgtag cccgtgatgt tgatgaaccc
     5161 cgaggccttc accttgggcg agctgagggt ctccacgacg atgttgaagg gcttgttcgt
     5221 ctctatgagg cgcttgaggt tcttctcgag cctgtcgtcg accaggaagg tgtgaatgag
     5281 ctcgtagagg gatttcccca cgaagggcat cgtggagggg tccatgttgg actcgaggac
     5341 cttgttgatg tagacgaccg tgccgctcct gtcgaacgcg cacacggcga acggcgcctt
     5401 ctcgaagacg gtccgtacgt tttcggtatg gtccttcatg gagcgctcag ccgatggtga
//
LOCUS       DAOSCR010000099         5458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9439_length_5458_cov_3.376643, whole genome shotgun sequence.
ACCESSION   DAOSCR010000099 DAOSCR010000000
VERSION     DAOSCR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9439_length_5458_cov_3.376643"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3424)
                     /locus_tag="PLT69_03840"
     CDS             complement(<1..3424)
                     /locus_tag="PLT69_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13606.1"
                     /translation="MTGSLLVYQVDYLMFVASFLLALGSIVCLVSYLDKIKRTVWAFM
                     GLFCMCNALFWFLCLLTLSMEGVTLAFEILRWSVFTLSFLALGELARRTEAFEGGIHA
                     RVKLITPLVLAGAIGLFFGSEAFKVFLHIGVAVPLGLWASLRIWKERRDGKAYGITSI
                     ATACFVVVACLVSLVGFSGAMHRGGTHSGSLSAMLLTLDLFSLCLLVSIIFSSRSRRD
                     IPSNAKEAGLWLWVILGVFLVGGGAFSAWVGFKADASLRAEVITQARIASQAIDPLSV
                     MDLTGTESDISSPIYQYIKRKLVAVRQANPFFRFVYLMGRKGSVVYFIADSEPPESKD
                     YSPPGQVYEHDVSSEFLKTFDQGLEITEGPTTDRWGTWVSASIPVRDPLSGRVVAILG
                     ADVDARDWSRKIFEAKRQPILGVMLVTMLTVVFMLERRREAIVSARVQSSERRLNYAL
                     EAASEGIWDWDIKTGETFYSPHWVASLGFSRGEMPPLAVLRETLIHQDDLEKARRSLS
                     DHLSGKSEFHECEFRTRKKDGGFIYTLERGKVVERDELGEPVRMVGTFTDITQRKEME
                     KDLRIKDEQYRQLVEHAGDIIVSTDNQGIITFINPACEKLLGYTTEEMLGRHYIDFVR
                     PESRKEVSRVTGRQFVKKVPRIYSEIPLVTKDGREVWTGQNISLLMDGDTVTGFHAIS
                     RDITERRLAELALKKSEEQYRHLVENAADVIFETDAGGYITFVNPAGERFSGYTREEF
                     VGRHYSEFLREDFKQATEKATGRQFVKRIPSIYLEVPIIRKDGRVVWMGESIQLITDH
                     ADRVTGFHVIARDITERKLIEDALRESEERLHAVFDHVQAGIVLIDPENHTVVSANRM
                     AARMCGHDTDEMQGKVCHEFLCPAEAGHCPVTDHGERIENMEKILLTRDGRRIPILKT
                     VIPIQISGKEFLLESFVDISDRKRAEEALLKSNEELEKTNRLLEEANRVSLEMSVKAE
                     MASQAKSLFLANMSHEIRTPMNAVIGMSELLMATDLSPEQSMYVGIINKSADALVELI
                     NDILDFSKIEADKVELEHIDFDLRSMLEDLVDLLSIRASERGIELNFLIDRDVPVMLV
                     GDPGRLRQVLTNLVGNAVKFTHEGEVFIHVQLGERSGDEVTLRFSVHDTGIGI"
     gene            complement(3668..4810)
                     /locus_tag="PLT69_03845"
     CDS             complement(3668..4810)
                     /locus_tag="PLT69_03845"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOJ13607.1"
                     /translation="MNPQGPVKTQILKVPRPFRLESGDTLEGLTIAYEAYGELSPSGD
                     NAVLIEHALSGDAHAAFYHEGARKPGWWDPMIGPGKAFDTNRYFVVCSNVIGGCSGTT
                     GPSSLNPATGRPYGLTFPPVTIRDMVEVQRLLLAHLGVKRLRCVAGGSMGGMQTMQLF
                     ADYPFFADSFVCIASTMRHSAQQIAFNEVGRRAIMADPRWNGGNYYGDVPPEGGLSVA
                     RMIGHITYLSERMMQEKFGRARKGKDHTVVFRPSFEVEHYLDYQGVSFVRRFDANSYL
                     YITSAIDNFDLVSSISQVGRFRGEGRARGTARDAMKAKKGLVVAFTSDWLYPPSQSRE
                     IARFFSGLGVAVSYAEIATDHGHDAFLTDTEAQSRLVGNFLASLPA"
     gene            complement(4807..5415)
                     /locus_tag="PLT69_03850"
     CDS             complement(4807..5415)
                     /locus_tag="PLT69_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOJ13608.1"
                     /translation="MKHTHIDTSLIIESMIEPQSRVLDLGCGDGSLLSSLIARKRVEG
                     TGVEISHDMVASCLAKGLCVFQADIDKGLSHWEDATFDYVILKSTLEVIHRPYELILE
                     MLRVGRHALVTVSNFGYLANRIHTLLTGRMTPRIRAFSPWYETSVIRYVCMDEFFEAL
                     RRSGVRISEARYVTPFGSVSRKKPLFANLFVVEAVFLLTRTP"
BASE COUNT          999 a         1704 c         1658 g         1097 t
ORIGIN      
        1 ggatgccgat ccccgtgtcg tgcaccgaga acctgagcgt cacctcgtcg ccggatcttt
       61 ccccgagctg aacgtggatg aagacctctc cttcgtgggt gaacttcacg gcgttgccca
      121 cgaggttcgt gaggacctgc ctcagcctcc ccgggtctcc cacgagcatg acagggacat
      181 ccctgtcgat gaggaagttg agctcgatac ccctttcgga cgccctgatg gagagcagat
      241 cgacgaggtc ctcgagcatg gatctcagat cgaagtcgat gtgttcgagc tccaccttgt
      301 cggcctctat cttggagaag tcgaggatgt cgttgatgag ttccacgagg gcgtccgcgc
      361 tcttgttgat gatgcccacg tacatggatt gctcgggcga caggtccgtg gccatgagga
      421 gttcgctcat gccaatgacc gcgttcatgg gggtgcgtat ctcgtggctc atgttggcca
      481 gaaagaggct cttggcctgg ctcgccatct cggccttgac cgacatctcg aggctgaccc
      541 tgttcgcctc ctcgaggagc ctgttcgtct tctcgagctc ctcgttcgac ttcaacaggg
      601 cctcctcggc gcgcttgcgg tccgagatgt ccacgaagct ctcgaggagg aactccttcc
      661 ccgagatctg gatgggtatc acggtcttga ggatggggat cctcctgccg tctcgggtca
      721 gcaggatctt ttccatgttc tcgatcctct cgccgtggtc ggtcacgggg cagtgtcccg
      781 cctcggccgg gcacaggaac tcgtggcaca ccttgccctg catctcgtcc gtgtcgtggc
      841 cgcacatcct tgcggccatc ctgtttgcac tcaccaccgt gtggttctcg gggtcgatga
      901 ggacgatccc tgcctggaca tggtcgaaca ccgcgtgaag gcgttcctcg ctctctctga
      961 gtgcgtcctc gatcagcttc ctctccgtga tgtcgcgggc gatgacgtgg aagcccgtga
     1021 ccctgtcggc atggtcggtg atgagctgga tgctctcgcc catccagacg acgcggccgt
     1081 ccttgcgtat gatgggcacc tcgagataga tgctcgggat ccgcttcacg aactgcctgc
     1141 ctgtggcctt ctcggttgcc tgcttgaaat cctccctgag gaattcggaa tagtgcctgc
     1201 cgacgaactc ctccctggtg tacccggaga atcgttcgcc cgcagggttc acgaaggtta
     1261 tgtatccccc ggcgtccgtc tcgaagatga cgtccgccgc gttctcgacc aggtgccggt
     1321 attgttcctc gctctttttc agcgcaagct cggcgagtct ccgttccgtg atgtcccgcg
     1381 atatggcatg aaacccggtg accgtgtccc cgtccatgag cagggagatg ttctggcctg
     1441 tccacacctc ccttccgtcc ttggttacca ggggtatctc ggagtagatc ctggggacct
     1501 tcttgacgaa ctgtcgcccc gtgaccctcg acacctcctt ccgggactcg ggcctcacga
     1561 agtcgatata gtgacggccg agcatttcct cggtggtgta ccctaaaagt ttttcgcatg
     1621 ccgggttgat gaaggtgatg atgccctggt tgtccgtgct cacgatgatg tccccggcgt
     1681 gctcgacgag ctggcggtac tgctcgtcct tgatccgcag gtctttctcc atctccttgc
     1741 gctgcgtgat gtccgtgaac gtgccgacca tgcgcacggg ctctccaagc tcgtcccgtt
     1801 ccaccacctt gccgcgctcc agggtgtata tgaagccgcc gtctttcttc ctggtcctga
     1861 actcgcactc gtgaaactcg gacttgcccg agaggtggtc gctcagagac cttctggcct
     1921 tttccagatc gtcctggtgg atgagggttt ccctcagcac ggccaggggc ggcatctcgc
     1981 ccctggagaa cccgagggac gcaacccagt gggggctgta gaaggtctcc cctgtcttga
     2041 tgtcccagtc ccagattccc tcgctcgccg cctcgagggc atagttcaac cgcctttccg
     2101 agctctggac cctcgccgag acgatcgcct cacgcctcct ctcgagcatg aagaccacgg
     2161 tcagcatggt cacgagcatg acaccgagga tgggctgtct cttcgcctcg aagatcttcc
     2221 tggaccagtc cctcgcgtcc acgtctgcgc ccaggatcgc caccaccctg cctgagagag
     2281 ggtctcgaac aggtatcgat gcgcttaccc acgtacccca ccggtcggtc gtcgggcctt
     2341 cggtgatctc gaggccctga tcgaacgtct tgaggaactc ggaagagacg tcatgctcgt
     2401 acacctggcc gggcggggag tagtccttcg attcgggcgg ttcggagtcg gcgatgaagt
     2461 agaccaccga tcccttcctg cccatcaggt acacgaaacg gaagaacggg ttggcctgcc
     2521 tgacggcaac gagcttgcgc ttgatgtact ggtagatggg agaggagatg tcagactcgg
     2581 tgcccgtgag atccatcacg gagagggggt ctatggcctg ggacgctata cgcgcctggg
     2641 tgatcacctc tgctcgcagg gacgcatcgg ccttgaaacc gacccacgcg ctgaatgcgc
     2701 cgccccccac gaggaacacg ccgagaatga cccacagcca caatccggcc tccttcgcgt
     2761 tcgaagggat gtccctcctc gacctcgacg agaagatgat ggagaccagg aggcacaacg
     2821 agaagaggtc aagcgtcagc agcatggccg acagggaacc ggagtgggtc ccaccccggt
     2881 gcatcgcccc actgaagccg accagcgaaa cgaggcaggc aacgacgacg aagcatgcgg
     2941 tcgcgatcga tgtgatgccg tatgccttgc cgtcccgcct ctccttccag atgcgcagag
     3001 acgcccacag gcccaagggg accgccacgc cgatgtgcag gaacaccttg aaggcctctg
     3061 acccgaagaa cagcccaatg gcacctgcaa gcaccaaggg ggtgatcagc ttcaccctgg
     3121 cgtggatgcc gccctcgaag gcctctgtac gcctggcgag ttctcccagt gccaggaaag
     3181 acagcgtgaa gacggaccac ctgaggatct cgaatgcgag agtgaccccc tccatgctca
     3241 gggtcaggag gcagagaaac cagaacaggg cgttgcacat gcagaacagc cccatgaacg
     3301 cccagacggt gcgctttatc ttgtccaggt aggatacgag gcacacgatc gaacccagcg
     3361 ccagcaggaa cgacgcaacg aacatcaggt agtcgacctg gtacacgagc agggatcccg
     3421 tcatgatata ctcgtatcga ttcattgaag ggccgacttt atggtctgcc cccgtggacg
     3481 ctccttcccc gtcattcaag gcaacagggc atcacgcacc acctgcggtg ggggggatcc
     3541 acggggagtc tcgccgggtg gcgatgggcc gagggtgcag caccgtgcag catgtcaagc
     3601 atggaggatg tcgggggagt tcccgaggtc aatcgcttgt gtgtaaaaaa gtctacactg
     3661 aacgctgtca agccgggagg gaggcgagga agttaccgac gagacgcgac tgggcctcgg
     3721 tgtcggtgag gaatgcgtcg tgaccgtggt cggtggcgat ctccgcatac gagaccgcca
     3781 cgccaagccc ggaaaagaag cgcgcaatct ccctggactg cgaaggcggg tacagccagt
     3841 cggaggtgaa cgccacgacg agccccttct tggccttcat cgcgtccctc gcggtgcccc
     3901 gggctcggcc ttcacccctg aacctgccta cctggctgat cgaggagacc aggtcgaagt
     3961 tgtctatggc gctcgtgatg tagaggtagc tgtttgcgtc gaagcgcctg acgaatgaca
     4021 caccctggta gtcaaggtag tgttccacct cgaaggaagg tctgaacacc acggtgtggt
     4081 ccttgccctt gcgggccctg ccgaacttct cctgcatcat ccgttcggag aggtaggtga
     4141 tgtggcctat catcctggca accgacaggc ccccctcggg gggcacgtcg ccgtagtagt
     4201 tcccgccgtt ccaccgcggg tccgccatga tggccctcct gcccacctcg ttgaaggcga
     4261 tctgctgcgc cgagtgacgc atggtcgagg cgatgcacac gaaggagtcg gcgaaaaagg
     4321 gataatccgc gaagagctgc atggtctgca tgccgcccat ggacccgcct gccacacacc
     4381 tgagcctctt gacgcccagg tgggcaagga ggagccgctg aacctccacc atgtcccgga
     4441 tggtgacggg ggggaaggtg aggccgtagg gccgtccagt cgccgggttc aaagacgagg
     4501 ggcccgtggt cccgctgcag ccgccgatga cgttcgagca caccacgaaa tacctgtttg
     4561 tatcgaaggc cttgcccggg ccgatcatgg gatcccacca gccgggtttt ctcgcaccct
     4621 cgtgatagaa ggcggcgtgc gcgtcgccgg acagggcgtg ctcgatgaga acggcgttgt
     4681 cccccgacgg gctcagctcg ccgtaggcct cgtaggcgat cgtcaggcct tcgagggtgt
     4741 cccccgactc cagcctgaaa ggcctgggga ccttgaggat ctgagtcttg acggggcctt
     4801 gcgggttcat ggcgtcctcg tgaggagaaa caccgcctcc acgacgaaca ggttggcgaa
     4861 cagcggtttc tttcggctca ccgaaccgaa cggggtcacg tacctcgctt ccgatatcct
     4921 gacaccggat cgacgcaagg cttcgaagaa ttcgtccatg cacacgtatc gtatcaccga
     4981 cgtttcgtac cagggagaga aggcgcgtat ccggggcgtc atccttcctg tgaggagggt
     5041 gtggatcctg ttggccaggt acccgaagtt ggagacggtc acaagggcgt gcctgcccac
     5101 cctgagcatc tccaagatga gctcgtacgg cctgtgtatg acctcgaggg tggacttgag
     5161 tatcacgtag tcgaacgtcg cgtcctccca gtgggacagc cccttgtcga tatccgcctg
     5221 gaagacgcac agccctttcg cgaggcagct ggccaccatg tcatgggata tctcgacccc
     5281 ggtcccctcc acccgcttgc gggcgatcag cgagctcaag agcgacccgt cgccgcagcc
     5341 gagatcgagc acccggcttt gcggctcgat catggactcg atgatgaggg acgtgtcgat
     5401 gtgggtgtgt ttcatccagc cttcaccatg ccttgtcctg tcgtcttgcg gtcaatcc
//
LOCUS       DAOSCR010000100         5456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9442_length_5456_cov_3.959637, whole genome shotgun sequence.
ACCESSION   DAOSCR010000100 DAOSCR010000000
VERSION     DAOSCR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9442_length_5456_cov_3.959637"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLT69_03855"
     CDS             complement(<1..336)
                     /locus_tag="PLT69_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13609.1"
                     /translation="MRDRSYVSGIMLFVFFMVIACAGSALAGKGQERLQRTETHKFTI
                     YEAPGGEDDPYSVRFPITLDEPGLITVDVEVGGGRIKHDGSPFKVWIVEAAGLRDEKT
                     NKINNKYIKK"
     gene            complement(535..609)
                     /locus_tag="PLT69_03860"
     tRNA            complement(535..609)
                     /locus_tag="PLT69_03860"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Thr,seq:cgt)
     gene            complement(711..1433)
                     /locus_tag="PLT69_03865"
     CDS             complement(711..1433)
                     /locus_tag="PLT69_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ13610.1"
                     /translation="MVEMRGVTKYYGALKALDNVDLDVYEGERVVIIGPSGSGKSTLL
                     RSINRLEEINAGTIVVDGFDIYDPQVNINKVREEVGMVFQQFNVFPHKTVLENLTLAQ
                     IVVRKRSRKEAEDIAYALLDKVGIREKAHVYPTKLSGGQQQRVAIARALAMQPRLMLF
                     DEPTSALDPEMIGEVLDVMVNLAKEGMTMIVVTHEMGFAREVADRVIFMDFGKILEEG
                     SPEHFFVSPTHERAQQFMRQIL"
     gene            complement(1447..2253)
                     /locus_tag="PLT69_03870"
                     /pseudo
     CDS             complement(1447..2253)
                     /locus_tag="PLT69_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861744.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(2250..2987)
                     /locus_tag="PLT69_03875"
     CDS             complement(2250..2987)
                     /locus_tag="PLT69_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ13611.1"
                     /translation="MVCMVFSFVLGAVLPADVSAAQPKKVVVATDATWPPMEMLDANK
                     NIVGFDIDYMNAVAKEAGFQVEFKNTAWDGIFGGLETGNYDAIISSVTITDERKKKYD
                     FSLPYINAGQVLVVPKTTKGVKTLADMKGKSVGAQIGTTGAMEVQKVKGVELKNYDEI
                     GLAFEDMAAGRIAGVVCDTPVAADYALQRAEYKAKFMIVGEPFTQEQYGIVVKKGNKA
                     LLDLINKGIKAVQKKGIDKQLEKKWLR"
     gene            3331..4011
                     /locus_tag="PLT69_03880"
     CDS             3331..4011
                     /locus_tag="PLT69_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13612.1"
                     /translation="MARTSEKRREETRDRIIMAACELFSKHGYHNTQVMDIVKAVGMS
                     AGTFYNHFKDKRDLFEQITLESLEGLRVTVRSLREPVDIWSPESRFKILQDTFNAIFD
                     YVDANPQQILMLLRVSFGVDEKLDGSVWDYFKGFAQDAGEDIQRWIDEGVLEGVDPML
                     FGTAVSGMVLQLIHNYLVEKSFRREDIVANLIKMILAMFEAYLTEDGRKAVDMNLLKS
                     QYPGPAAT"
     gene            4757..>5456
                     /locus_tag="PLT69_03885"
     CDS             4757..>5456
                     /locus_tag="PLT69_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13613.1"
                     /translation="MARDLDRLFMVARSQTGQVGAEVTDLLRQWVNREVVSVRMEYRK
                     EYGKLFEQRWRSLATDLSIARLTMSESRGGFGWNKAGDAAGLARAAWEIGRADVSLGL
                     LAAGTWIVHLLGGDTPCLEASPVSGGAEGAPKASFVLPGPGMSGESDPLFSGRAVRAR
                     AVRTKTGYELSGRLLRPLWAGAVADVHVIVCALDDEPCIAFVRAGAEGLARGPALSTT
                     GLNACTNADLTLERV"
BASE COUNT         1016 a         1734 c         1673 g         1033 t
ORIGIN      
        1 tttcttgatg tacttgttgt tgatcttgtt cgtcttctcg tccctgagac ccgcagcctc
       61 gacgatccat accttgaacg gcgatccgtc gtgctttatc cttccccctc ccacctcgac
      121 gtccacggtg atgagcccgg gctcgtccag cgtgatgggg aatctcacgg aatacgggtc
      181 atcctcgcca ccaggcgcct cgtagatcgt gaacttgtgg gtctccgtcc tctggaggcg
      241 ttcctgcccc ttgccagcca gggccgatcc cgcgcatgca atcaccatga agaacacgaa
      301 cagcatgatg ccggacacgt acgatctgtc tctcacgctc aacctcccag gtgttttccc
      361 catgataccc catcatgcag ggaacttcca gatcgagacg tcacctgggg gggggggcga
      421 ggagggccgt cctgcaagga cggtaagtcg gccgaggctg ggagagagag gatccagcga
      481 aggtggtcga acgtaaacca aggtggggca catccaacca acgccggaaa agcgtggagc
      541 cagcgaaggg ggtcgaaccc ttgacctgct gattacgaat cagctgctct accactgagc
      601 tacgctggca caggtgatgt ttttggcaca gacacaaatt gcttgtcaat ggtgtatgca
      661 ggccgcgaga acgcaccgga tctcatgccg gccccactgg ccgcctcgct tcacaggatc
      721 tggcgcatga actgctgggc ccgctcgtgg gtcgggctca cgaagaagtg ctcgggagac
      781 ccctcctcca ggatcttccc gaagtccatg aagatgacgc ggtcggccac ctcgcgggcg
      841 aaccccatct cgtgggtcac cacgatcatg gtcatgccct ccttcgcgag gttcaccatg
      901 acgtcgagga cctcgccgat catctccggg tcgagcgccg aggtgggctc gtcgaagagc
      961 atgagcctcg gctgcatggc cagggccctg gcgatggcca ccctctgttg ctggccgccg
     1021 gagagcttgg tcgggtatac gtgggccttc tcgcggatcc ccaccttgtc gagaagggcg
     1081 taggcgatgt cctccgcctc cttgcgcgag cgcttcctga ccacgatctg ggccagcgtg
     1141 aggttctcga ggaccgtctt gtgggggaac acgttgaact gctggaagac catgccgacc
     1201 tcttcgcgga ccttgttgat gttcacctgc gggtcgtaga tgtcgaaccc gtcgaccacg
     1261 atggtgccgg cgtttatctc ctcgagcctg ttgatggacc tgaggagggt gctctttccg
     1321 gagccggacg gcccgatgat gaccaccctc tcgccttcgt agacgtcgag gtccacgttg
     1381 tcgagggcct tcagcgcgcc gtagtacttc gtgacgccgc gcatctccac catggtcttc
     1441 cttgtgtcag tccgcacgaa tcttctcctc catgatgctg atgagtttcg agaagaacag
     1501 ggtgatcatg aggtagacga gcgccaccat cgtgtaggtc tcgaagtagg tgaagctctg
     1561 cgaggcgaac tcgcggcccc gtctcagcaa gtccgccacg gcgaggatcg acacgagcga
     1621 cgagtccttg agcagggcca cgaactcgtt tcccacaggc ggcaggatcg tcttgaccgc
     1681 ctgcggcagg atgacgtggt acatggactg ggcgggggtc attcccaggg acctggctgc
     1741 ctcgctctgg cccttcggaa tggactggat gccggccctg aagatctctc ccatgtaggc
     1801 gccgtaacag atggccatgg ccgcaaccgc gctcgtcagg ggcggcagct agaagagccg
     1861 gccgaggccg tagtagatgt agaacagctg caccaagagc gggatacccc tgatcacctc
     1921 gacgtagagg gaggcgaggc cgtttacgag cctgttcgac gaaagcctgc cgaggcccgt
     1981 gaacaggcct atgaccaggg cgatgacgat cgacaagacc gtgacctcga aggtcacgag
     2041 tatcccgtca gggacgaact tcatgatctc gaggtaaggg tccggctaga agatgcagag
     2101 ggacagcgtc agcgcgatcg acccgaaaaa ggcgacgcgc cacgcggtga agaggccgac
     2161 atcctccttg gtggggatgg cggccccgtc accgacctcg atctgtacct tcggccgggc
     2221 ctcttcctcg acggccttct tcctgggcgc tatctcagcc acttcttctc gagctgcttg
     2281 tctatgccct tcttctggac agccttgatc cccttgttga tgaggtcgag gagcgccttg
     2341 ttccccttct tcaccacgat gccgtactgc tcctgcgtga agggttcacc gacgatcatg
     2401 aacttggcct tgtactcggc cctctgcagg gcatagtccg cggcgacagg cgtgtcgcac
     2461 accacgccgg ctatgcggcc cgcagccatg tcctcgaagg cgagcccgat ctcgtcgtag
     2521 ttcttgagct ccacgccctt gaccttctgg acctccatgg cccctgtcgt gccgatctgc
     2581 gcacccacgc tcttgccctt catgtcggcc aacgtcttga cgcccttggt ggtcttcggg
     2641 accacgagca cctggcctgc gttgatgtag gggagcgaga agtcgtactt cttcttgcgc
     2701 tcgtcggtga tggtgacgga cgagatgatc gcgtcgtagt tgcccgtctc gagccccccg
     2761 aagatcccgt cccacgcggt gttcttgaac tccacctgga agcccgcctc cttggcgacc
     2821 gcattcatgt agtcgatgtc gaagcccacg atgttcttgt tcgcatcgag catctccatg
     2881 ggaggccagg tcgcgtcggt tgcgaccacg accttcttcg gctgggcggc cgaaacgtcc
     2941 gcgggcagaa cggcacccaa gacgaacgag aagaccatgc ataccatgca tacaagagca
     3001 cttctcttca ttggatatcc cccctctttt attttggagt tctttctcgt cggacaagca
     3061 cgggaaagcg actcggaatg actaccacag attcgggcgc cagtccagaa tcatgaaccg
     3121 caccccgatc cacgcgccgg ggaggcgatc aactctcagg tgcatgcggg gcagccggat
     3181 gcggcggtcg gcgatgtgga cacggcggtg gtcgaaggcc ctcacgaacc tttcctcgag
     3241 gcaaatcaac gcatttcggt tgacatccgc caaccctgga catatctaac agggcatggg
     3301 catgtctgcc ttgaacgatc ggggttgacc atggcgagga catccgaaaa gagacgtgag
     3361 gagacgaggg accgcatcat catggcggcc tgcgagctct tcagcaagca cggctaccac
     3421 aacacccagg tcatggacat cgtcaaggcg gtgggcatgt ctgcaggcac cttctacaac
     3481 cacttcaagg acaagcgcga cctcttcgag cagatcacgc tcgagagcct cgagggcctc
     3541 agggtcacgg tcaggagcct gagggagccg gtcgacatct ggagccctga gtcacggttc
     3601 aagatcctcc aggacacgtt caacgccatc ttcgactacg tggacgcaaa cccccagcag
     3661 atcctcatgc tcctcagggt cagcttcggc gtcgacgaga agctggacgg gagcgtctgg
     3721 gactacttca aggggtttgc gcaggacgcc ggcgaggaca tccagaggtg gatagacgag
     3781 ggggtcctcg agggcgtcga ccccatgctc ttcggcaccg cggtgagcgg catggtgctg
     3841 cagctcatcc acaactacct ggtggagaaa tcgttcaggc gcgaagacat cgtggccaac
     3901 ctcatcaaga tgatcctggc catgttcgag gcctacctca ccgaggacgg ccgcaaggcc
     3961 gtggacatga acctcctcaa gagccagtac cccgggccgg ccgcgacctg acgggaccca
     4021 tcccccctga gaaccgaggc agtttcacgt cttgttccat aacggtcaca atgtccgaat
     4081 accgccgccg tccttcgaaa gagaaccgag gcgttcccac agggggacca cgatcccctg
     4141 cccccggccc cgggctgaag gaaagccgtg atgcctctcc ttcccgaacg cgggctcctg
     4201 gccatcggcc ggaagtcgct ttccccggcc gccgtccctt tatccccacc gtgggccgca
     4261 gggtgaccac cgcgcacccc cttgtccttc gggcgcccct tcctcccaaa cgttggctgt
     4321 agttctccgc agccggcgtg agaggttccc ggagatcccc tttcacccca ccgtgggccg
     4381 tagttctccc cagccggcgt gaaaggttcc cggagagccc atttctcccc accgcagacc
     4441 gcaggcctgg accgaccacc catgcaaccg ccaatcgccg acggacaggc gtctcgatcc
     4501 cctcttgaca tcgtgagtaa tgataattat cattcagcat aatgaggttt cattattaaa
     4561 ccacccgtgg atggtgggtg gagtagtgag gcgagaactt ggcatggact tcgagggtgt
     4621 acggggacac cgatgctgat cggccgtgac gatcgtcttc ccgctcttac cgtgaatgcc
     4681 gatccctcgt ccccgcgcgg gggtcgcgcc cccgaggcga tgccgggcaa ggaaaccccg
     4741 caccgaggag gaacgcatgg caagagacct tgatcgtttg ttcatggtgg cccgttcgca
     4801 gactgggcag gtcggcgctg aggtgacgga cctcctcagg cagtgggtga acagggaggt
     4861 cgtgtcggtc cggatggagt accgcaagga gtacgggaag ctcttcgagc aaagatggcg
     4921 ctcgctcgcg acggacctct cgatcgccag gctcaccatg agcgagtccc ggggagggtt
     4981 cgggtggaac aaggcagggg atgcagccgg cctggcacgt gccgcatggg agatcggcag
     5041 ggccgacgtc tccctcgggc tgctggcggc cgggacctgg atcgttcacc tcctcggcgg
     5101 cgatacgccc tgcctcgagg catcgccagt ctcgggcggt gccgaaggcg caccgaaggc
     5161 gtccttcgtc ctgccagggc cgggcatgtc gggagagtcg gatcccctct tctcgggaag
     5221 ggccgtcagg gcgcgcgcgg tcaggacgaa gaccggctac gagctgtccg gacgcctcct
     5281 gagacccctg tgggccgggg cggtggcaga cgtgcacgtc atcgtctgcg cccttgacga
     5341 cgagccctgc atcgccttcg tgcgagccgg ggccgagggc ctcgcgagag gccccgccct
     5401 ctcgacgacg ggcctgaacg cgtgcacgaa cgcggatctc accctcgagc gcgtgc
//
LOCUS       DAOSCR010000101         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9463_length_5448_cov_3.813833, whole genome shotgun sequence.
ACCESSION   DAOSCR010000101 DAOSCR010000000
VERSION     DAOSCR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9463_length_5448_cov_3.813833"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PLT69_03890"
     CDS             <1..1329
                     /locus_tag="PLT69_03890"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HOJ13614.1"
                     /translation="RLRPRSRALFEEAKRNWTGGVPMSWMRIWPGGFPVFVAEAEGVT
                     VVDVDGHSYLDLCLGDTGGLCGHAHPKIVEAIYDQMRRRGTTTMLPTEDVIWVGRELE
                     HRFSLPAWQVLMTATDANRMALKVARSFTGRQLVLCFNGTYHGSVDETLVVNFFGELV
                     TIPGMLGPIVKDASLMTKVIDFNDVDALEEALAPGDVACVITEPVMTNIGIIYPQPGY
                     HEALRRLTRKYGTLLLIDETHSLCAGPRGVTGEMGLEPDIFVAGKFIAGGYPAAVLGF
                     TREISDWMASRLPWHNFFGFGGTLSGNATAVAGIRAALEHVLTDENFSRMKALASRME
                     QGLARVIAGHGLEWYVARIGCRVEFRFLPKPPKNGSEALFSEVDYNAVDIVSEGLTGP
                     LDALIHVWCANRGILLTPVHEMTLVGPAATERDVDRYVDTIAGLVEELVS"
     gene            1571..2338
                     /locus_tag="PLT69_03895"
     CDS             1571..2338
                     /locus_tag="PLT69_03895"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOJ13615.1"
                     /translation="MKRLAVVCIVIAAAVSAWTIQASAMDGNEVLKKVDEVQSASKDS
                     VADIVMILKGKDATTKERSMKVWRRHYRDKDDWSLLKFVSPADIKNLGFLSLADDKMY
                     LYLSAFDRVRRIASHARKESFAGSDLSNDDLSTGKYSSHYDAKIVREEGDDYVLELKR
                     KPSSERIYPRVTAWVDKKEFTVSKMDLYDDSNRLWKTSTIKNERIQGYWTPVEISMKD
                     VTKDHTTIMKISKITYDTGLKDEIFTERYLKRDVKGD"
     gene            2331..3920
                     /locus_tag="PLT69_03900"
     CDS             2331..3920
                     /locus_tag="PLT69_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13616.1"
                     /translation="MIEGRLTLPRAHDAQACPALRREGHPGSPACAKHAAAFVCAAAL
                     VLACAATLKAQDEGSRSFDLKGTVKLYGSVYTEDDASDRYDPHEALDYAINRGELHLK
                     LDGYVSEMVSYKSHLAFTYGMRHDVRDLSQLEVQESGTEQETDLSTELKAASITVMDL
                     GTAGVDLTVGRQRIRWGTSDEYNVIDNLNPVDYGNVFSFDPDYFVEHLPMDGFNLEYR
                     LPTEWELKAQFVYLLSFRPASLPEGFKERITDLMEDELEEQTAPYGFPTGEVSVDLDS
                     SPEYRAGKGTVGVRVGGNAFNMDWGVSYFHGYAGLPLIHAISSDITLVEGTTLNALMS
                     YPRLDVLGFDLAGEVRSVGVWAEAGCYWPEDYVVRRTAKLMSTTRTDVIRLFDRPYWK
                     YTLGFDYTFGIGSGLYWNVQFNHGFYDEFRYTPEAGSELGLGRPGFMGGIEDYLATRV
                     EYKFFHDELTTVLDCLLEMPDKSDVNGYSAVVVKPKLIYKPYDSTSFELAYVLITGDS
                     RTKYGAFEKNDVVYLLTKVYF"
     gene            3974..4240
                     /locus_tag="PLT69_03905"
     CDS             3974..4240
                     /locus_tag="PLT69_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ13617.1"
                     /translation="MERDDPGRVMQRSITTHPEKCTGCLRCALACSFRHTGMFNPMRA
                     RIHIRMDGPACDISFDDACTGCLACVGGCLYGALAAERQREGSA"
     gene            4237..>5448
                     /locus_tag="PLT69_03910"
     CDS             4237..>5448
                     /locus_tag="PLT69_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ13618.1"
                     /translation="MSSKGFAGRTAIVDLGTGAVEVRRLDEREAERFGGGLGLCIALA
                     ARAIPGPHGALGQENAVVIGAGPFVGTDLPATSRVYAVSALPCSNSIGWCGAGGMTFG
                     CGLKYAGFDHVVVKGASSRPVYLSIHDDGVDMLDASWLSGKGAGRTCEELKARHQAQA
                     GVLAVGPAGERLVSFAMAYVDGVSTLGRGGLGAVMGSKNLKAVVAGGSRGIGVADPVL
                     YRSLRDGLVERIRRYPYLASWQDLGLLSSLPVVPAEVYRRLRVRRLACVSCPLGDKDM
                     LKIEGAAPGDIVCSSSAVNLFMPLVYGMTDPLDAARCIARLDELGMDMFEFFGIMRLA
                     ASLQEMGLISGDPVIDVSSPSSMLSWADKVVERDGVGDTLAGGFDRIVREFGEQALRC
                     APPTFRGMVTYV"
BASE COUNT          961 a         1710 c         1875 g          902 t
ORIGIN      
        1 aggcttcgcc cgaggtcgag ggcgctgttc gaggaggcga aaaggaactg gacgggcggc
       61 gtgcccatgt cgtggatgcg catctggccc ggcggcttcc cggtgttcgt cgctgaagcc
      121 gaaggggtga ccgtcgtgga cgtcgacggc cacagctacc tggatctctg cctcggggac
      181 acggggggcc tgtgcggcca cgcccacccg aagatcgtcg aggccatata cgatcagatg
      241 aggaggcgtg gcaccacgac tatgctcccc accgaggacg tgatctgggt gggcagggag
      301 ctcgagcaca ggttttccct ccccgcgtgg caggtgctca tgaccgcgac ggacgcgaac
      361 cgcatggcct tgaaggtggc gaggtccttc acgggcaggc agctcgtgct ctgcttcaac
      421 gggacgtacc acgggtctgt cgacgagacg ctcgtggtga acttcttcgg cgagctcgtg
      481 acgatccccg gcatgctcgg accgatcgtg aaggacgcct ccctcatgac gaaggtcatc
      541 gatttcaacg atgtcgacgc cctcgaggag gccctcgcgc cgggtgacgt cgcgtgcgtc
      601 atcacggagc ccgtgatgac gaacatcggc atcatctacc cgcagcctgg ctaccacgag
      661 gccctgagga ggctcacgag gaagtacggg acgctcctgc tcatcgacga gacgcactct
      721 ctgtgcgcag gcccccgagg ggtgaccggc gagatggggc tcgagcctga catcttcgtg
      781 gcaggcaagt tcattgccgg cggttacccg gctgcggtgc tcggtttcac ccgcgagatc
      841 tcggactgga tggcatcccg gctgccgtgg cacaacttct tcgggttcgg gggaaccctc
      901 tcgggaaacg ccaccgcggt cgccggcatc cgggcggcgc tcgagcacgt gctgaccgac
      961 gagaacttct cgcgcatgaa ggcgctggcc tcgcgcatgg aacagggtct cgcgcgggtc
     1021 atcgccgggc acgggctcga gtggtacgtg gcgaggatcg ggtgccgggt ggagttccgc
     1081 ttcctcccga agcccccgaa gaacgggtcg gaggcgctct tctccgaggt ggactacaat
     1141 gccgtcgaca tcgtgagcga ggggctcacg gggccgctcg acgcactcat ccacgtctgg
     1201 tgcgcgaaca ggggcatcct cctgaccccg gtgcacgaga tgaccctcgt ggggcccgcc
     1261 gcgaccgagc gggacgtgga caggtacgtg gacaccatcg cggggctcgt cgaagagctc
     1321 gtgagctagg gcaccggctg ccccccgtcg cccatgcccg cgacgtcgag ccggccttgc
     1381 cggccgaggc gcgcccgggt tcgagggggg atgcctcgcg gcgaagatcc cgtgggccgg
     1441 ggcgggggac gtcgccggtg atgagggtcg gcgacgccgc cccgcaggcg gacagccggt
     1501 gttcggggct tggaagacgc tgcccgatat ggcagaacag gcagagcata agattctccg
     1561 ggaggtgctg atgaaaaggc ttgctgtggt atgcatcgtc atcgcggcgg cggtctcggc
     1621 ctggacgatc caggcgtccg ccatggacgg gaacgaggtc ttgaagaagg tggacgaggt
     1681 ccagtcggcg tccaaggact ctgtcgcgga catcgtcatg atcctgaaag gcaaggacgc
     1741 gacgaccaag gaacggagca tgaaggtctg gagacgccat tacagggaca aggacgactg
     1801 gtccctcctc aagttcgtct cgcccgcgga catcaagaac ctcgggttct tgagtctggc
     1861 cgacgacaag atgtacctct acctgagcgc cttcgacagg gtgaggagga tcgcgtccca
     1921 cgcgaggaag gagagcttcg caggctctga cctctccaac gacgacctga gcaccggcaa
     1981 gtactcgagc cactacgacg cgaagatcgt gagggaggaa ggggacgact acgtgctcga
     2041 gctcaaacgg aaaccctcct ccgagcgcat ctaccccagg gtcacggcat gggtggacaa
     2101 gaaggagttc acggtaagca agatggatct ctacgacgac tcgaacaggc tctggaagac
     2161 ctcgaccatc aagaacgagc gcatccaggg gtactggacc cctgtcgaga tctcgatgaa
     2221 ggacgtgacc aaggaccaca cgacgatcat gaagatctcg aagatcacct acgataccgg
     2281 gctcaaggac gagatcttca cggagcgcta cctgaagagg gacgtgaagg gtgattgagg
     2341 gccgcctgac cctgccccgc gcgcacgacg cccaggcctg ccccgccttg agacgcgagg
     2401 ggcatccggg gtctcccgca tgcgcgaagc atgccgccgc cttcgtgtgc gccgcggcgc
     2461 tcgtgctcgc gtgcgcggcg acgctcaagg ctcaggacga aggctcgcgc tcgttcgacc
     2521 tgaaggggac ggtcaagctg tacggttccg tctacaccga ggacgacgcg tccgataggt
     2581 acgaccccca cgaggccctg gactatgcca tcaaccgtgg cgagctccac ctcaagctcg
     2641 acggctacgt ctcggagatg gtcagctaca agtcacacct ggccttcacc tacggcatgc
     2701 gccacgacgt ccgggacctc tctcagctcg aggtgcagga gagcggcaca gaacaagaga
     2761 ccgatctctc caccgagctg aaggcggcct cgatcaccgt catggacctc gggacggcag
     2821 gggtcgacct caccgtggga cgacagcgca tccgctgggg tacctccgac gagtacaacg
     2881 tgatcgacaa cctgaacccc gtggactacg gcaacgtctt ctccttcgac ccggactact
     2941 tcgtggagca cctgcccatg gacgggttca acctcgagta ccgcctcccc accgagtggg
     3001 agctcaaggc ccagttcgtc tacctcctct ccttcaggcc cgcttccctg cccgagggct
     3061 tcaaggagag gatcaccgac ctcatggagg acgagctcga ggaacagacg gccccgtacg
     3121 ggttccccac gggggaggtg agcgtcgatc tcgactcttc gcccgagtac agggccggga
     3181 aggggaccgt gggggtgagg gtcgggggga acgccttcaa tatggactgg ggcgtgagct
     3241 acttccacgg ctatgccggc ctgccgctga tccatgcgat ctcctcggac atcaccctcg
     3301 tggaggggac gaccctgaac gccctcatgt cctacccgcg cctggacgtt ttgggattcg
     3361 acctggcggg agaggtccga tcggtgggcg tctgggccga ggccggctgc tactggcccg
     3421 aggactacgt cgtgaggagg acggcaaagc tcatgtccac gacgaggacc gacgtcatcc
     3481 gcctcttcga ccgaccctac tggaagtaca cgctcggatt cgactacacc ttcgggatcg
     3541 ggtcgggcct gtactggaac gtccagttca accacggctt ctacgacgag ttcaggtaca
     3601 ccccggaggc ggggtccgag ctgggcctcg ggcggccggg gttcatgggc ggtatcgagg
     3661 attacctggc gacccgcgtc gagtacaagt tcttccacga cgagctcacg accgtgctcg
     3721 actgcctcct ggagatgccc gacaagagcg acgtgaacgg gtactccgcg gtggtggtga
     3781 agccgaagct catctacaag ccgtacgaca gcacctcctt cgagctcgcc tacgtcctga
     3841 taacgggcga cagtcggacg aagtacgggg cgttcgagaa gaacgacgtg gtgtacctcc
     3901 tcacgaaggt ctacttttag ggccctctca ccccgatgcc gccgttggtg ggcgtgagag
     3961 gcggaggctc ccggtggaga gggacgatcc cggcagggtg atgcaaagat cgatcacgac
     4021 gcatcccgag aagtgcacgg gatgcctcag gtgcgcgctc gcctgctcgt tcaggcatac
     4081 gggcatgttc aaccccatgc gtgcgaggat acacatccgc atggacgggc ctgcctgcga
     4141 catctcgttc gacgacgcct gcacgggatg cctcgcctgc gtcgggggct gcctctacgg
     4201 cgcgcttgcg gcggaacgcc agagggaggg gtccgcttga gctcgaaggg gttcgcggga
     4261 aggacggcga tcgtcgacct cggtacaggt gccgtggagg tgcgccggct tgacgagcgc
     4321 gaggccgagc ggttcggggg aggcctcggc ctgtgcatcg ccctggccgc acgcgccatc
     4381 cccggccccc atggcgcgct ggggcaggag aacgccgtcg tcataggagc cggcccgttc
     4441 gtggggacgg atcttcccgc gacctcgcgc gtctacgccg tctccgcgct tccctgcagc
     4501 aactcgatcg ggtggtgcgg cgccggcggg atgacgttcg ggtgcggcct gaagtacgcg
     4561 ggcttcgatc acgtggtcgt gaagggggcg tcttcccgtc ccgtgtatct ctcgatccac
     4621 gatgacggcg tggacatgct cgacgcgtcc tggctgtcgg gaaaaggggc gggacggacg
     4681 tgcgaggagc tcaaggccag gcaccaggcc caggccgggg tcctggcggt cggccctgcg
     4741 ggcgagcggc tcgtgtcctt tgccatggcg tacgtggacg gcgtctccac cctcggcagg
     4801 ggcgggctgg gcgcggtcat gggctccaag aacctcaagg ccgtcgtggc gggtggaagc
     4861 aggggtatcg gggtagccga ccccgtcctc taccgctcgc tgagagatgg cctcgtggag
     4921 aggatacggc gctaccccta cctggcgtcc tggcaggacc tcggcctcct gtcctccctg
     4981 cccgtggtgc ctgcggaggt gtacaggcgg ctcagggtca ggaggctcgc gtgcgtgtcg
     5041 tgccccctgg gcgacaagga catgctcaag atcgaggggg cggctccggg ggatatcgtc
     5101 tgctcgagct ccgcggtgaa cctcttcatg cccctcgtct acggcatgac cgacccgctg
     5161 gatgcggccc ggtgcatcgc acgcctcgac gaactgggca tggacatgtt cgagttcttc
     5221 gggatcatga ggctcgccgc ctccctccag gagatgggcc tgatctcggg cgatccagtc
     5281 atcgacgtgt catcgccgtc gtccatgctc tcgtgggcgg acaaggtggt cgagagagac
     5341 ggcgtgggtg atacgctcgc aggcgggttc gaccggatcg tgagggagtt cggcgagcag
     5401 gccctccggt gcgccccccc gaccttcagg gggatggtca cctacgtg
//
LOCUS       DAOSCR010000102         5447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9469_length_5447_cov_2.828450, whole genome shotgun sequence.
ACCESSION   DAOSCR010000102 DAOSCR010000000
VERSION     DAOSCR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9469_length_5447_cov_2.828450"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1146
                     /locus_tag="PLT69_03915"
     CDS             373..1146
                     /locus_tag="PLT69_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOJ13619.1"
                     /translation="MTIAEEAAVRAFDAAPGAFRRSIDVAQAKAGMPPGSGFARLLPR
                     EPASALTHAPGIALAVAGTWHLLARALWPVRPWHLAGYAVFGLCGVLLYAASTLFHWA
                     RASKDTISALRKLDHIMIFVFIAATYTPFCLVPFRGPFGLFMLSCIWCIAAAGSVVKL
                     SWMHMPRGLCVSLHLFAGFFSMIGIGRIISVLGPHAVSLLFTGGAFYCIGAALYLLES
                     DRDLSSPKLFGLHEAFHLMVLLGGACHYMCIARYVDTFA"
     gene            complement(1285..2061)
                     /locus_tag="PLT69_03920"
     CDS             complement(1285..2061)
                     /locus_tag="PLT69_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ13620.1"
                     /translation="MAVVLGAGASKGFAHIGVLKVLESQKVPVHMVVGTSAGSLVGSL
                     YASGVDPFALQDMALKIQKKDVIDYTVPDNGFIKGEKLEDFINKTVRNTPIERLKKKF
                     LAVATDIATGEEAVFVAGNTGKAVRASCSVPGVFQPVKMGGKTYVDGGVVSPVAVEAA
                     RRHGADVVIAVDISGGIAGEVPQGTLDTILQSVDIMYAKISEHQLSKADVVIRPDVRG
                     IGSTEMEKRHQAILEGEKAAYAALPRIMKVLEERRSASRP"
     gene            2653..2892
                     /locus_tag="PLT69_03925"
     CDS             2653..2892
                     /locus_tag="PLT69_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOJ13621.1"
                     /translation="MAQRGRKPLELRTRDKDVKLIKRNYYIDERYAKALSVISALTGK
                     ELSLLVNEAIGGLIKKYQPQTDIDLMKLKTIDNEK"
     gene            3626..4525
                     /locus_tag="PLT69_03930"
     CDS             3626..4525
                     /locus_tag="PLT69_03930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOJ13622.1"
                     /translation="MTARDSLRVWILAIVLLAACATGFTAHGKPDGPSVTTYRISGVD
                     PGFNLSREEVARAVREAASIWEEAAGRPLFREDADGHLEIKLVYDHRQAVSDTLSGLK
                     SRLAGKQVAYEALKSQITQLESEYHSQRDALLSEMSEYNARMGAYSAAMRSSADHGPQ
                     KALEQEYEALEAMYHTLSRKAHELAATEHSLSDMIAVLNEMAQEMNKDAQGMRTVRMS
                     AGAEFSEACYELRKDGSETITVYHFADRTRLVRVLAHELGHALGLEHSRDPDGIMYRM
                     NTSTNLKPSADEVAALRRLLEER"
     gene            4797..>5447
                     /locus_tag="PLT69_03935"
     CDS             4797..>5447
                     /locus_tag="PLT69_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOJ13623.1"
                     /translation="MTRLELLAPARDLECAIAAIDHGADAVYIGGPSFSARKAASNPM
                     AHIERLARHAHRFHARVYLALNTILFEDELEAARKTAHEAWDAGVDGLIVQDMGLLEL
                     DLPPVPLIASTQMHNMDPEHVAFLERVGFSRVILARELSLKAIQRIRSAASIELEVFV
                     HGALCVSFSGRCYLSASMGGRSANRGECGQPCRLPWTLRDDRGRPLTQGLHLLSLKD"
BASE COUNT         1018 a         1837 c         1586 g         1006 t
ORIGIN      
        1 atgcgcccct gccaccctgg tacccatctt catccttcgt tgtgcccggg ctcgggcctc
       61 acgggttgta tttccatgga gcccctcaca tccaccggga aacatggttg ttttgcttta
      121 ttatgaaata tcttgccctt tttgtggagt cctttacctc ggcttctttg cgccggtttg
      181 cccacgcatg cggggccccc atgctgtatc ttgattttct ttgccaaatc atgctgcagg
      241 gacaggcggg gcgctggcac gacctgtgaa tggagcgctc cagttgtgtt cgattgaagc
      301 ccgaagggac ctgtgacgat gcagcgaaga gagtgggcgc caggggcaac agaggcgaat
      361 ccgccgcaca cgatgaccat tgccgaggaa gcggcggtac gcgcattcga tgcagcgccg
      421 ggtgcgttta gacggagcat cgatgtcgcc caggcaaagg cgggcatgcc ccccggcagc
      481 gggttcgccc gtctcctccc gagggaaccg gcgagcgcgc tcacacatgc gcccgggatc
      541 gcgctcgccg ttgcgggcac gtggcacctg ctggcgcgcg cgctgtggcc ggtgagaccg
      601 tggcacctgg cagggtatgc cgtcttcggc ctgtgcggcg tcctcctcta tgccgcgagc
      661 acgctctttc actgggcacg cgcctccaag gacacgatct cggccctcag gaagctcgat
      721 cacatcatga tcttcgtctt catagccgcc acctacacgc ctttctgcct cgttccgttc
      781 aggggtccgt tcggattgtt catgctctcg tgcatctggt gcatcgcggc tgcgggctcg
      841 gtagtgaaac tctcctggat gcacatgccg agaggcctgt gcgtttccct gcatctcttt
      901 gcggggtttt tctccatgat cgggataggc cgcatcatct ccgtgctcgg gccccacgcg
      961 gtctccttgc tcttcacggg cggggccttc tactgcatcg gcgccgctct ctacctgctg
     1021 gagagcgatc gagatctatc cagtccaaag ctcttcggtc tccacgaggc cttccacctc
     1081 atggtcctgc ttgggggagc ctgccactac atgtgcatcg cgcgctacgt cgacacgttc
     1141 gcctgagccg aagaatcctc atgtcccgcc tgggaggtca ggcaaaagcc agcacggtca
     1201 ttgcggacga aaaagccgaa gggctccttc cccgcgagga gacaggtaca gcgccacgca
     1261 tgaggctcac gagccctcgg gtgctcaggg cctcgatgcg gatctcctct cttcgagcac
     1321 cttcatgata cgaggaaggg cggcgtatgc ggccttctct ccctcgagaa tggcctggtg
     1381 cctcttctcc atctcggtgg accctatgcc cctgacatcg ggacggatca ccacgtcggc
     1441 cttcgagagc tggtgctcgc tgatcttggc gtacatgatg tccacggact gcaggatggt
     1501 atccagcgtc ccctggggca cctcgccggc gatccccccg gatatgtcga cggctatcac
     1561 cacgtccgcc ccatgcctcc ttgccgcctc gaccgccacc gggctgacca cgccgccgtc
     1621 gacgtacgtc ttgccgccca tcttcacggg ctggaagacc ccggggaccg agcagctcgc
     1681 cctaacggcc ttgccggtgt tgccggccac gaacaccgcc tcctcgcccg tcgctatgtc
     1741 ggtcgcgacc gcgaggaact tcttcttcag tctctcgatg ggcgtgtttc tgaccgtctt
     1801 gttgatgaag tcctcgagct tctcaccctt gatgaacccg ttgtccggca ccgtgtagtc
     1861 gatcacgtcc ttcttctgta tcttcagggc catgtcctgg agggcgaacg ggtcgacgcc
     1921 ggaggcgtac aggctcccca caaggcttcc ggcgctggtt ccgacgacca tgtgaacggg
     1981 caccttctgg gactcgagca ccttgagcac gcctatgtgg gcaaacccct tcgacgcccc
     2041 tgccccgagg accaccgcca ccttcacctc cttggtcggg ggcgtggtct ccaccccggg
     2101 gggggtcggc ctcgcggggc acccggcccc gacaacggcc aggagcgccg ttacaaagag
     2161 gacactcgct ctcagaagcc ctttcctcgg cacaggcatg gcatccttca tcgtgcagcc
     2221 cctttctgac ggagtttctt cggaatacgg ccccgccctc aggacagccc cctccatgca
     2281 acaccggccc tcacaccggg ggatatcccg ggaagccgcc ttccggacgc accccagata
     2341 gtccctccgg agtcgcctgt caaggacgag cgcggacttc cccgcccgtg aactgaggct
     2401 cggccttcag gatgcagcga gcccgcaacc tgcgcgcggg gtttggacgc acgcgtagac
     2461 cacaggccgg gtcaacatcc agcgggtcac ccatatgcgc cacctacatc aatgatttac
     2521 cctgaacgcc ggtcaaccta tatgagtttc tccttggtct tatgaaatcc gaaatctctc
     2581 ttgattctcg taacgccatg ggatagagtg cgtcccaatt tatcatcatt ctatacccca
     2641 aggagagtgc acatggcgca aagaggaaga aagccgcttg aactgcgtac gagggacaag
     2701 gatgtgaagc tcatcaaaag aaactattac atcgatgagc gttacgccaa ggcgctttcc
     2761 gtgatctcgg ccttgaccgg gaaagaactg tctctcctcg tgaacgaggc tataggcggc
     2821 ctcatcaaga aataccagcc gcagacagat atcgatctca tgaagctgaa gaccatcgac
     2881 aacgagaagt gacccgtctc ccgcacacga ggccatcaca aggacgacga acacgggccg
     2941 cttgacgccc ctgacgcgat acagtcatgc acacagcccc gagaagaagg gaacctgacc
     3001 cctaaagccc ggcaccctgc aggaaataga agaccaaggg cggttcactc cccatacccc
     3061 tactaggtga accctcactt cgggctctca cgtaaccctt ccggagacag gagaaccatg
     3121 ggcagggagt gcatctttcc cgggcacgac cgaaggacga ccctgccaca ccgtccccac
     3181 ccggctgtcg taagatcgcc agagtgaggg tcgtgcggcg acgtgcggtc ggagccgcgg
     3241 gagaccatcg ccgcatcacg gccacgctga accacgggtt catgcctgga ggcacagagt
     3301 ccccgacgca tcttctctgc agccttgagg ctgccgtcgc caggcagccg tgagggctgg
     3361 gaaaccggtc cctcatcgct gcagggccgc ccgagcggtg cacgaacccg ccggcacaca
     3421 gaccatgcaa catgcctggc cgatgactac aggcctgtct ttcccatgcc ggtcaaggcg
     3481 tggggtcttc ccaagcccct ttgcgcacca cctcgctgtc ttcccttccc tgcgccgggc
     3541 gcgaccaggc aggccaagga atcaccatgc atttcgatgc cgcagagacg ctcaagcaag
     3601 tgccgtttcg tccgaaaggt tctgtatgac cgcacgggat tcgctcaggg tatggatact
     3661 cgccatcgtc ctgcttgccg cctgcgccac ggggttcacc gcgcacggga agcctgacgg
     3721 cccttcagtg accacgtacc gcatctccgg ggtggacccg gggttcaacc tgagccgtga
     3781 agaagtggcc agggctgtgc gcgaggctgc atccatctgg gaggaggctg caggcagacc
     3841 cctgttcaga gaggacgccg acggtcatct cgagataaaa ctcgtctacg accaccgcca
     3901 ggcggtatcc gacaccctca gcggcctcaa gtcccgcctc gccggcaaac aggtcgccta
     3961 cgaggccttg aagtcccaga tcacccagct cgagtccgaa taccattccc agagggacgc
     4021 gctcctctcg gagatgtccg agtacaacgc gcggatgggg gcatacagcg ccgcgatgcg
     4081 ctcttctgcc gaccatgggc cccagaaggc cctcgaacag gagtacgagg ccctcgaggc
     4141 catgtaccac acgctgagca ggaaggccca cgagcttgcg gcaaccgaac attccctctc
     4201 cgacatgatc gcggtcctca acgagatggc ccaagagatg aacaaggatg cacaagggat
     4261 gcggaccgtc cgcatgtcgg caggtgccga gttcagcgag gcctgctacg agctccggaa
     4321 agacgggagc gagacgatca ccgtctacca tttcgccgac cgcaccaggc tcgtgcgcgt
     4381 gctcgcccat gagttgggcc atgcgctcgg gctcgagcac tcgcgggacc ccgacggcat
     4441 catgtaccgc atgaacacct cgacgaacct gaagccgtcg gcggatgagg tcgcagccct
     4501 tcgccgtctc ctcgaggaaa ggtagacgcg gcgtatccta taagccgcgt cgaaacggcg
     4561 cgccacccct gaacgtatcg gaaccatgcc catttccgcc ccggcttcct gtccccgggc
     4621 acccgaatgc gaggatcttg ccatcatcgc caggcgggtc aacctctcac ccggcatcaa
     4681 ggtccgatgt gcccgggtcg gtatcctttc ttttccgaac ccgcgggaag cggcctcttc
     4741 acgctgctgt gggaagtctt gccgttcgag gcaatgtggg gtaggttcaa caccctatga
     4801 cgagattgga gctccttgcg ccggccagag acctcgaatg cgccatcgcc gcgatagacc
     4861 acggggccga tgccgtctac atcggcgggc cctcgttcag cgcacgcaag gccgcctcca
     4921 accccatggc acacatcgag cgcctggcac gccatgccca caggttccac gcgagggtct
     4981 acctcgcact gaacaccatc ttgttcgagg acgagctcga ggccgcccga aagaccgccc
     5041 atgaagcatg ggatgccggg gtcgacggcc tcatcgtcca ggacatgggg ctcctcgaac
     5101 tcgatttgcc gcccgtgcct ctcatcgcga gcacccagat gcacaacatg gatcccgagc
     5161 acgtcgcctt cctggaacgc gtgggcttca gcagggtgat cctggccagg gagctctcgc
     5221 tgaaggccat ccagcgcatc aggtcggcgg cgagcatcga gctcgaggtg ttcgtgcacg
     5281 gcgccctgtg cgtctctttc agcggcaggt gctacctgag cgcatccatg gggggcagga
     5341 gcgcgaacag gggcgagtgc ggccagccct gcaggctccc ctggaccctc agggacgacc
     5401 ggggaaggcc gctcacccaa ggactccacc tcctgagcct caaggat
//
LOCUS       DAOSCR010000103         5428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9506_length_5428_cov_3.453564, whole genome shotgun sequence.
ACCESSION   DAOSCR010000103 DAOSCR010000000
VERSION     DAOSCR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9506_length_5428_cov_3.453564"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1884)
                     /locus_tag="PLT69_03940"
     CDS             complement(247..1884)
                     /locus_tag="PLT69_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ13624.1"
                     /translation="MEYSTSPDGKRYPLPGKDDYLEEIGRLEKLVASHRARGSEIVVV
                     MGLGFVGAVMAAVVAGSTDRTTGRPKKFVIGMQRPSPRSFWKIHKLNEGVSPVKAEDP
                     EVDVLIRRCVLEQKNLVATYTYDALGLADVVVVDVQCDFLKSELGNCTTGSAEIAALE
                     ESMKILAERIDPRCLVLIETTVPPGTTEYIAYPILKRAFRKRGIEGEPLLAHSYERVM
                     PGKDYVRSIRDFWRVCSGINEESKARVIGFLSDVLNVEEYPLTVLDRPIESETAKIVE
                     NSYRATILAFMDEWSTFCEKNGVDLVKVIQAIKVRPTHSNMLFPGPGIGGYCLPKDGG
                     LGVWAYRHLMGFTDEIFRMTPLAIDINDTRALRAVQLVRDALRNMGKIVAASTVCVLG
                     ASYREDVGDTRYSGSEVIVRKLVEMGADIRVHDPYVDHWWELEAQDAYPAVGAGLARF
                     FRNQDMLRDLHVGKDLAETLQGAEAVVLAVRHRHYLELDPDEVVGMIGSPAAVVDCFG
                     ILDDARIWRYFELGCEVKGLGRGHIQRIKEEVRSRRE"
     gene            complement(2035..3603)
                     /locus_tag="PLT69_03945"
     CDS             complement(2035..3603)
                     /locus_tag="PLT69_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ13625.1"
                     /translation="MHTVKVAVIGAGSWGRNLVRNFNELGALHALCDSNDAVIAELGR
                     LYPSIPRTTDYRSILEDDAVRGVVISTPASTHFSLAREALLSGKDVFVEKPLALDVDE
                     AYELRDIAAGKGGVLMVGHLLQYHSAFVKLKELVLQGELGRINYVYSNRLSFGKIRRE
                     EDIMWSFAPHDISMILSLAREVPESVMATGGNYLHKKIADVTTTHLDFPSGLKAHVFV
                     SWLHPFKEQMLVVVGDKNMAVFRDTAQWEEKLLLYPHDIHWEGGVPVPDRKEAVAVRV
                     ETIEPLKAECRHFLECIASRRTPVTDAEEGIAVLRVLKAGQESLNAGGKRVFLAQEGR
                     GPSGIMVHESSYVDQDVTIGEGTRIWHFCHILKGSRIGRGCSIGQNVVIGPDVTIGNN
                     CKIQNNVSVYKGVTLEDDVFCGPSMVFTNVFNPRAHIPRMDELRPTVVKRGATIGANA
                     TIVCGHTIGAYAFIGAGSTVTRGAPDHALMVGCPARQIGWVCECGNRLDDALSCSHCG
                     KVYERDERGLRLRA"
     gene            complement(3637..4791)
                     /locus_tag="PLT69_03950"
     CDS             complement(3637..4791)
                     /locus_tag="PLT69_03950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ13626.1"
                     /translation="MQFIDLAAQQERIRGKIVEKIEAVLAHGQYILGPEVREIEERLA
                     AFVGVKHALSCASGTDALLMALMAYGVGPKDAVFTTPFTFVATAEVISLLGATPVFVD
                     IEPDTFTIDADGLEKAIDALDAQDPSRHSLPKGFEGLRARGIIAVDLFGQCADYRRIR
                     EIAQRRGLFLVEDAAQSFGATYSGKRSCSLADIAATSFFPAKPLGCYGDGGMVFTDDD
                     GLAHALESIRVHGKGSDKYDNVRIGINGRCDTLQAAILLAKFEIFPEEIELRQAAASR
                     YGELLGDVDGITCPVVRPGCTSVWAQYSPLCANRAKVLDALREAGIPTAIYYPKPLHV
                     QAAYAYLGYRPDDFPVSMSVASRIFSLPMHPYLSRRDQERIADVIRNAAR"
     gene            complement(5219..>5428)
                     /locus_tag="PLT69_03955"
     CDS             complement(5219..>5428)
                     /locus_tag="PLT69_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOJ13627.1"
                     /translation="GEPLMVALETAHIQSPAQCRSGECSLCRTKLLSGTVFHPEGARL
                     RKSDRQFGYIHPCVAYPLEDLEILI"
BASE COUNT          897 a         1830 c         1665 g         1036 t
ORIGIN      
        1 cgtcaagtga gggaagacat caggtcatca ctgcaccttg cacccagtcg ccccacggtc
       61 gtcccgtcca gggcgatcgt ccgcagcatc acctggggcc gcctctcccg gcaggcgaag
      121 acgggcacga gacaagggcc ccgggcatgt tccgccacgg cagcctgcac aaagacgcgt
      181 gccggacccg gcatttcttt gtcctcccgg ccgccttccc tccaggtgaa cgcctccctt
      241 gcccgatcac tccctccttg acctcacctc ttccttgatg cgctggatgt gtcccctccc
      301 gaggcccttg acctcgcagc cgagctcgaa atagcgccat atcctggcgt cgtcgaggat
      361 gccgaaacag tcgacgacgg ccgcaggcga accgatcatg ccgacgacct cgtcggggtc
      421 cagctcgagg tagtgcctgt gcctgaccgc gagcacgacc gcctccgccc cctgaagcgt
      481 ctcggcgagg tcctttccca cgtgcagatc cctgagcatg tcctggttcc tgaaaaaacg
      541 cgccaggccg gcgcccacgg cagggtaggc gtcctgggcc tcgagctccc accagtggtc
      601 gacgtacggg tcatggaccc tgatgtccgc gcccatctcc acgagcttgc gcacgatgac
      661 ctccgagccg ctgtagcgcg tgtcgcccac gtcttcccgg taggaggcgc cgagcacgca
      721 cacggtggag gcggcgacga tcttccccat gttcctcagg gcgtccctca ccagttgaac
      781 ggccctgagc gcgcgcgtat cgtttatgtc gatcgccagc ggggtcatgc ggaagatctc
      841 gtcggtgaac cccatgaggt ggcggtacgc ccacacgccg aggccgccgt ccttgggcag
      901 gcaatacccg ccgatgcccg gaccgggaaa gagcatgttg gaatgcgtcg gcctgacctt
      961 gatagcctgt atcaccttga cgaggtccac cccgttcttc tcgcagaacg tgctccactc
     1021 gtccatgaag gcgaggatgg tcgcccggta gctgttctcg acgatcttgg ccgtctcgct
     1081 ctctatcggg cggtccagga cggtcagggg gtattcctcc acgttcagga catccgagag
     1141 gaagcctatc acccttgcct tgctctcctc gttgatcccg ctgcagaccc gccagaagtc
     1201 cctgatggat ctcacgtagt cctttcccgg catgaccctc tcgtagctgt gcgccaggag
     1261 gggttcgccc tcgatgcccc tcttcctgaa cgccctcttc aagatggggt acgcgatgta
     1321 ctcggtcgtg ccgggcggca ccgtggtctc gatgagaacc aggcacctgg ggtcgatcct
     1381 ttccgccagg atcttcatcg actcctcgag cgccgcgatc tccgcgctgc ccgtcgtgca
     1441 gttgccgagc tccgacttca ggaaatcgca ctggacgtcc accaccacga cgtccgcaag
     1501 gcccagtgca tcgtacgtgt aggtcgcaac gaggttcttc tgctcgagga cgcagcgcct
     1561 gatgaggacg tccacctcgg ggtcctccgc cttgacggga gagaccccct cgttcagctt
     1621 gtgtatcttc cagaagctcc tggggctcgg cctctgcatc cctatcacga acttcttcgg
     1681 cctgcccgtg gtcctgtcgg tgctgccggc cacgaccgcg gccatgacag cgcccacgaa
     1741 accgaggccc atcaccacga cgatctcgct tccccgtgcc cggtggctcg ccacaagctt
     1801 ctcgagccgg ccgatctcct ccagatagtc gtccttccct gggagagggt atctcttccc
     1861 gtcaggactc gtggaatatt ccatgtaccg tcacctccat aggatgcatc agtgcacgaa
     1921 atggccccgt cccggcctcg acgcgggctg cggcatgccg ctcaacgaac cttgggaacc
     1981 gtggagcagg cgagcgcccc tgccaacgcc acgatgtcag ccggcgccgt ttcgtcatgc
     2041 cctgagtctc aggcccctct cgtccctctc gtagaccttg ccgcagtgcg agcacgacag
     2101 ggcgtcgtcg agccggttgc cgcactcgca gacccagccg atctgccgtg ccgggcaacc
     2161 gaccatgagc gcatgatcgg gcgccccccg tgtcaccgtg gagcccgcgc cgatgaaggc
     2221 ataggcgccg atggtgtgac cgcagacgat cgtcgcgttg gcgcctattg tcgcgcccct
     2281 cttcaccacc gtcggcctga gctcgtccat gcgggggatg tgggcgcgcg ggttgaagac
     2341 gttcgtgaag accatggacg gcccgcagaa gacatcgtct tcgagggtca cgcccttgta
     2401 cacggacacg ttgttctgga tcttgcagtt gttgccgatg gtcacgtccg ggcctatgac
     2461 gacgttctgg cctatactgc accccctgcc gatccgcgat cccttgagga tgtggcagaa
     2521 gtgccagatc ctggtgccct ctcctatcgt gacgtcttga tccacgtagc tcgactcgtg
     2581 aaccatgatc ccggagggcc ccctgccttc ttgtgccagg aagaccctct ttccgcctgc
     2641 attgagagac tcctgacccg ccttgagcac cctgagcacg gcgatgcctt cctcggcgtc
     2701 tgtcacgggg gtgcgcctcg aggcaatgca ctcgaggaag tgccggcact cggccttcaa
     2761 gggctcgatc gtctccaccc tcacggcgac ggcctctttc ctgtcgggca cgggaacgcc
     2821 gccctcccag tgtatgtcgt gggggtagag caggagcttc tcttcccact gggcggtgtc
     2881 acggaacacg gccatgttct tgtcgcccac gaccaccagc atctgctcct tgaaggggtg
     2941 cagccaggag acgaacacgt gggccttgag cccgctcggg aaatcgaggt gggtggtcgt
     3001 gacgtcggct atcttcttgt ggagatagtt gccgcccgtc gccatgacgg actcggggac
     3061 ctccctggca agggacagga tcatggagat gtcatggggc gcgaagctcc acatgatgtc
     3121 ttcttccctg cgtatcttgc cgaaactgag acggttggag tagacatagt tgatcctgcc
     3181 gagctcgcct tgcaggacga gctccttcag cttgacaaag gcgctgtggt actggaggag
     3241 gtggcccacc atgaggacgc ctcccttgcc ggccgcgatg tctctcagct cgtacgcctc
     3301 gtccacatcg agggcgagcg gcttttccac gaacacgtcc ttgccggaca gcagggcctc
     3361 gcgcgcaaga gagaagtgcg tggaggcggg cgtggagatg acgacccccc tgacggcgtc
     3421 gtcttcgagt atggaccggt aatcggtggt tcttggtatg gatgggtaga gcctgccgag
     3481 ctcggcaatc accgcatcgt tgctgtcgca caaggcatgg agcgcgccga gctcgttgaa
     3541 gttcctcacg aggttcctgc cccaggaacc ggcgcctatg acggccacct tcacggtatg
     3601 cacggtatcc acctcatcct ccatcctcag gtcggttcac ctggcggcgt ttcgtatcac
     3661 gtccgctatc ctctcctggt cccggcggga gaggtacggg tgcatgggca gggagaagat
     3721 cctcgacgcg acgctcatcg agaccgggaa gtcgtcgggc ctgtacccca ggtatgcata
     3781 ggcggcctgc acgtgcaggg gttttgggta gtagatcgcc gttgggatgc ccgcctccct
     3841 caacgcatcg agcaccttgg cgcggttcgc acacaacggc gagtactgcg cccagacgct
     3901 cgtgcagccg ggcctgacga cagggcaggt tatgccgtcc acatcgccca ggagctcgcc
     3961 gtagcgtgac gccgccgcct gcctgagctc gatctcctcg gggaagatct cgaacttggc
     4021 gagcagtatg gcagcctgca gggtgtcgca cctgccgttt atgccgatgc gtacgttgtc
     4081 gtacttgtcg gaacccttcc cgtgcacccg gatcgactcg agcgcgtgcg ccagcccgtc
     4141 atcgtccgtg aacaccatgc cgccgtcgcc gtagcacccg agcggcttgg ccgggaagaa
     4201 ggacgtcgcc gcgatgtcgg cgagcgagca tgacctcttg ccggaatacg tggcgccgaa
     4261 cgactgcgcg gcgtcttcaa cgaggaacaa tccgcgcctt tgtgcgatct cccgtatcct
     4321 tcgatagtcg gcgcactgtc cgaagagatc gaccgcgatg atccccctcg ccctgagacc
     4381 ctcgaacccc ttcggcaggc tgtggcgcga ggggtcttgt gcatcgagcg catctatcgc
     4441 cttctcgagc ccgtcggcgt cgatcgtgaa ggtgtccggc tcgatatcca cgaacaccgg
     4501 ggtcgcgccc aggagcgata tcacctcggc tgtggccacg aaggtgaacg gcgtcgtgaa
     4561 caccgcgtct ttcgggccca cgccgtaggc catgagcgcc atgaggaggg catcggtacc
     4621 cgatgcgcac gagagggcgt gcttcacccc cacgaaggcc gcgagcctct cctcgatctc
     4681 tctgacctca ggcccgagga tgtactggcc gtgtgcaagc acggcctcga tcttctcgac
     4741 gatcttgccc cttatccgtt cctgctgcgc cgcaaggtcg ataaactgca tgtatccgtt
     4801 cctcgtcatg ggaagtgcaa cgccccgtac aggacggtga acccaagcct atgtgcgggt
     4861 gtgcggtcgt gcccgccccc ggcagtcctc cacccaaaca ccccgagggc aggtcattct
     4921 tatcacagtg atccgcgaag gccaagctga gttccatgcc ttccttgggt atcacgggct
     4981 gggctcgcgc cctgaggcgc ctgtaccggc tgagcgacga ggaggacgcc caatgccgcc
     5041 aggcatggcg ccaccggagg ggcgcatcga aggcggcaca ccagcggctt cccgcctgtt
     5101 cggctatgca tctcgagacg gctccggtgg gggcggttcg gtctcgacga gaggagtccc
     5161 gcccggccgt ctcggcatgg gcgcggacgg ccgggcatcg aggcaagcca tgtacggctc
     5221 agatgagtat ctcgaggtcc tcgagggggt aggccacgca cgggtggatg tagccgaact
     5281 gcctgtccga cttcctgagc cttgcgccct cggggtggaa cacggttccc gagaggagct
     5341 tggtgcggca gaggctgcac tccccggacc tgcactgggc cgggctctgg atgtgggccg
     5401 tctcgagcgc caccatgagc ggctcgcc
//
LOCUS       DAOSCR010000104         5414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9546_length_5414_cov_3.959134, whole genome shotgun sequence.
ACCESSION   DAOSCR010000104 DAOSCR010000000
VERSION     DAOSCR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9546_length_5414_cov_3.959134"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..706)
                     /locus_tag="PLT69_03960"
     CDS             complement(8..706)
                     /locus_tag="PLT69_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ13628.1"
                     /translation="MRHIRTIFLMTLAFISLFPAQCLAASLLVSWNANGEADLAGYKV
                     YCSTQSGVYGQGVDVGNVTSYSIPSVQAGTTYYVAVSAYDTSNNESEKSAEVSASIPV
                     SDTTPPTGSVVINAGAARTSSRTVTLTLSASDTQGAVTGMRFSNDGSTYSAETAYATT
                     YTWTLSSGDGVKTVYARFKDSSGNWSSAVSDSIELVTDTDGDGMPDAWENTYGLNPSA
                     NDASGDLDADGVSP"
     gene            992..2239
                     /locus_tag="PLT69_03965"
     CDS             992..2239
                     /locus_tag="PLT69_03965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ13629.1"
                     /translation="MDYAEERQVAQEFVDLLERNNLVIHDEEITVPVQILLERLAHRL
                     PNPLYRYRLHVVRDRSVNAFTIPDGHIFVNLGLLMFARDMDEIAAVIGHEMGHAQLRH
                     IPETLETQAKITAATALGILAGTLLSSKNPEAGAAMIFSSLGGGENLKLAYSRQHEFA
                     ADEFGAGILKSSGIDPSAMTRFLARLNATSGASGVPEYLLTHPLTDKRVAAIATDAGS
                     PRPDETYLRLQACIFGLLLPAQEASLRSSTLPEPYRSLAIALVKYRSGDYAGAMALLE
                     GIDLDIARATKGLVLYAMGRSEEAYPLLSSYGRTASARTAQAEILRGRGRYAEAIELL
                     KPYSGQNPRADYVLGVLYEGMGNQALSHVSFARYFFRSANRSPSLFHIQEALKRRDEL
                     PQGVAEELEKMREALRQEASERK"
     gene            complement(2635..4395)
                     /locus_tag="PLT69_03970"
     CDS             complement(2635..4395)
                     /locus_tag="PLT69_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOJ13630.1"
                     /translation="MKTSTHDLIVGIPTPVVPLVSMMVVGDRLDRKELAERLSDEGYL
                     AGTVSSLPERLSELLLSLHEAGGVAEEDMLARVMGYDADELKSSLEQLGRRGLVFQSG
                     LSGREPLLILPSLWPVMEALRSSRCRDPGTLEWEAVRSDGLWPHLTLINAVATFRIRC
                     KGGLEPFKKGWELLEAKLSHAMDLRRVYAELVELGCLREKDGFLVPVQRHVVSLAMGG
                     ALRYRVWRYYRSCRGRPGLDYKVFLAMGDRALQRDHLLRVVLLHVFAMERSLPDSERS
                     EIEALVDEWTGAGILEQDVSGAWLRFAPDVFEALRTGRAETPARFYSDEAIVQPTMEI
                     LVPRDFDPVDLVNVGEVADLVHADVVSIYRVTKDSVARAVQAGWDQDKIVRFLERISR
                     HSIPEALRMNVAGWCRLHAEAHLIRGTFLVFGGDKAVLPRGLDEVLPGIFRVPRNCEE
                     EIGAFLARKGVVLRDVDAEAEHDHEFDWGRTGPIAPPRPTAQTRIVMKEGVFPYGMVI
                     PMTFGPRRESLFEEALRMHRDIVIFYPRQGYGEMQVRKVCPLSIFRKGGVHFMEAYCD
                     DTKEGEVFDLGKVRAVLRHA"
     gene            complement(4376..>5414)
                     /locus_tag="PLT69_03975"
     CDS             complement(4376..>5414)
                     /locus_tag="PLT69_03975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ13631.1"
                     /translation="PCGAGKTVVGMAVMARARTHTLILCTNVSAVNQWVSELLDKTTL
                     CPSDIGVYIGGRKEIAAVTVTTYQMLTYRRSKDGPFEHMNLMMGRNWGLIVYDEVHVM
                     PAPVFRATTELQAKRRLGLTATLIREDGLEDDTFSLVGPKCYDVPWKELEQKGFIAQA
                     LCCEVRVPLASEKVREYVSSPERVQFRIASENGNKERVLTAILKKHMDEPVLVIGQYL
                     SQLKRIAARLKAPLITGSTSNARRDELYESFRRGDIPVLVVSKVANFAIDLPDASVAV
                     QVSGTFGSRQEEAQRLGRILRPKDRVSTFYTLVSKETSEEEFAHKRQVFLAEQGYRYR
                     IEEWTVEDLDT"
BASE COUNT          925 a         1833 c         1713 g          943 t
ORIGIN      
        1 agaataccta cgggctcacg ccgtctgcgt cgagatcacc cgatgcgtcg ttcgccgacg
       61 ggttgagccc gtaggtattc tcccaggcat cgggcatacc gtcgccgtcg gtgtcggtca
      121 cgagttcgat ggagtcgctc accgctgagc tccagttccc cgaggagtcc ttgaacctgg
      181 cgtacacggt cttcacgccg tcacccgagg acagcgtcca cgtatatgtc gtggcatagg
      241 cggtctccgc ggagtaggtg gacccgtcgt tgctgaaccg catgccggtc accgcgccct
      301 gggtgtcgga agcgctcaag gtgagcgtca cggtcctcga cgaggttcgc gcggcgccgg
      361 cgttgatcac gaccgaccct gtcggggggg tggtgtccga gacggggatg gaggcgctca
      421 cctcggccga cttctccgac tcgttgttgc tcgtgtcgta ggctgacacg gcgacgtagt
      481 acgtcgtgcc ggcctggacg ctcggtatgg agtacgaggt cacgttcccc acgtcgacgc
      541 cctgtccgta cacgccggat tgcgtcgagc agtagacctt gtaaccggcg aggtccgctt
      601 ctccgttcgc attccagctc accagcagcg atgccgcgag gcactgcgcc gggaacaggg
      661 agatgaaagc cagcgtcatc aagaagatcg tgcggatatg tctcatggtg atacctccgt
      721 cgtcacctgt gcgtacgttc cctcaggcaa gacggagacc aagggatata gtcttgtatt
      781 atcatcatgt tggagtgttc ccgcacgtaa tcgtccccac agttttgtac acgtggttac
      841 acatgcgtaa tccggggtcc gcgcggtgag cgcggcggtc aggatcgccc ttgaaaaagc
      901 ggaccggggg gcttacaaca cacccatcat gacccgctcg atggtcctat gctgggcttt
      961 catcctcgcg gtgacgcctt gggcccaggc catggattac gccgaggaga ggcaggtcgc
     1021 ccaggagttc gtcgacctcc tcgagaggaa caatctcgtg atccacgacg aggagatcac
     1081 cgtgccggtc cagatccttc tggaacggtt ggcccaccgc ttgccgaacc cgctctaccg
     1141 ctacaggctc cacgtggtga gggaccgctc ggtcaacgcc ttcacgatcc cggacgggca
     1201 catcttcgtg aacctggggc tcctcatgtt cgccagggac atggacgaga tcgccgcggt
     1261 cataggccac gagatgggtc acgcccagct ccgccacatc ccggagacgc tcgagaccca
     1321 ggcgaagata accgcggcca cagccctggg catcctcgcc gggaccctgt tgtcctccaa
     1381 gaaccccgag gcgggggcgg ccatgatctt ctcgtcgctc ggcggcggag agaacctgaa
     1441 gctcgcctac tcgaggcagc acgagttcgc ggcggacgag ttcggcgcgg gcatcctcaa
     1501 gtcctcgggc atcgacccgt ccgccatgac acgctttctt gcgaggctga acgccacgtc
     1561 gggtgcctct ggggttcccg agtacctcct cacacacccg cttaccgaca agagggtcgc
     1621 ggccatcgcg acggacgcgg ggtctccccg cccggacgag acctacctga ggctccaggc
     1681 gtgcatcttc gggctcctcc tccccgcaca ggaggcctcc ctgcgttctt ccaccctgcc
     1741 ggagccgtac aggtcgttgg cgatcgccct cgtgaagtac cgctccggcg attatgccgg
     1801 ggcgatggcg ctcctggaag ggatcgacct ggacatcgcg cgcgcgacaa agggcttggt
     1861 cctgtatgcc atggggagga gcgaggaggc ctaccccctg ctctcctcgt acggcaggac
     1921 cgcgtcggcg cggacagccc aggcggagat cctcaggggc aggggcaggt acgcggaggc
     1981 catcgagctg ctcaagccct acagcggcca gaacccgagg gcagactacg tcctgggcgt
     2041 cctctacgag ggcatgggga accaggccct ctcgcacgta tcgttcgcgc gctacttctt
     2101 caggtcggca aaccgctccc cgtcgctctt ccacatccag gaggcgctca agagacggga
     2161 cgaactcccc cagggggtgg ccgaagagct cgagaagatg agggaggccc tcaggcagga
     2221 agcctctgag cgcaagtgag gagaaaaagc ttggaggagc ccgcctgctc gaggccctgc
     2281 ttacgtgccg acagagcggc gatgcccggc tgcgggaggg aaagcccgga ggagcccgcc
     2341 cgtcgaaggc ctggacgcat ccttgctcct gtgatgttca tagtctctcg ggagaaaagc
     2401 ccggaggagc ccgcccgttg aaggcccctc acgcccgagg caccacgcct tcgggatcga
     2461 ccgggaggcc ggcacaggcg tgggccgggg gacgaggatg acgcagaccc ccttgtgcac
     2521 gcatgcgagg ggacctgagg ccgcacaccc ggcctgggac cggacagaca caccccacaa
     2581 cctcgagcac ccctccagga cacggcgaaa ggccgtggcc gggggcggcg gccctcacgc
     2641 gtgcctgagc acggccctca ccttgccgag gtcgaacacc tcgccctcct tggtatcgtc
     2701 gcagtaggcc tccatgaaat gcacgccccc cttccggaag atggacaggg gacatacctt
     2761 ccttacctgc atctctccgt agccttgccg ggggtagaag atgacgatgt ccctgtgcat
     2821 cctgagggcc tcttcgaaga gcgactcccg cctcggcccg aacgtcatgg ggatgaccat
     2881 gccgtagggg aagacgccct ccttcatgac gatgcgcgtc tgggctgtcg gtctcggtgg
     2941 tgctatgggg cccgtcctcc cccagtcgaa ctcgtggtcg tgctccgcct cagcatcgac
     3001 gtccctcagg acgacccctt tcctcgccag gaaggcccct atctcctcct cgcagttcct
     3061 ggggacgcgg aagatgccgg gaagcacctc gtcgagcccc ctcgggagca ccgccttgtc
     3121 gccgccgaac acgaggaacg tccccctgat gaggtgggcc tcggcatgca ggcggcacca
     3181 gccggccacg ttcatcctca gcgcctccgg gatgctgtga cgggagatcc tctccaggaa
     3241 cctcacgatc ttgtcctggt cccagcccgc ctggacggcc ctggccacgg aatccttcgt
     3301 gacgcggtat atgctcacca cgtccgcgtg gacgaggtcg gcgacctcgc cgacgttgac
     3361 gaggtcgacc gggtcgaagt ccctggggac gaggatctcc atggtgggct gcacgatcgc
     3421 ctcgtcagag tagaaacgcg ccggggtctc ggccctgccc gtcctgaggg cctcgaacac
     3481 gtccggggcg aacctcagcc acgcccccga cacgtcctgc tcgaggatcc ccgcgccggt
     3541 ccattcgtcc acgagcgcct cgatctcgga cctctcgctg tccgggagtg acctctccat
     3601 ggcgaacacg tggagcagca cgacccggag gaggtggtcc ctctgcaggg cacggtcccc
     3661 catggcgagg aacaccttgt agtcgagccc gggccgaccc ctgcacgaac ggtagtatct
     3721 ccagacgcga tagcggaggg cgcctcccat ggcgagcgag acgacatggc gctgcacggg
     3781 gaccaagaac ccgtctttct ccctgaggca cccgagctcg acgagttccg cgtagaccct
     3841 cctcaggtcc atggcatggg agagcttcgc ctcgaggagc tcccaccctt tcttgaacgg
     3901 ctcgagcccg cccttgcacc ggatcctgaa ggtggcgacc gcgtttatca gtgtgaggtg
     3961 gggccagagc ccgtccgacc tcacggcctc ccactcgagg gtgcccgggt ccctgcaccg
     4021 ggatgacctg agggcctcca tgaccggcca gagcgaaggc aggatgagga gcggttccct
     4081 gccgctcagg ccgctctgga aaacgagccc cctccgcccc agttgctcca gggaggactt
     4141 gagctcgtcc gcatcgtacc ccatcacccg cgcgagcatg tcctcctcgg cgacaccccc
     4201 cgcctcgtgg aggctcagga ggagctccga gagccgctcg ggcagcgagg agaccgtccc
     4261 ggcgaggtac ccctcgtcgg agagcctctc cgcaagttcc ttgcggtcga gcctgtcacc
     4321 caccaccatc atggacacga ggggcaccac gggcgtgggt atgccgacga tgaggtcatg
     4381 tgtcgaggtc ttcaaccgtc cactcctcga tgcggtaccg gtacccctgt tccgcgagga
     4441 agacctgcct cttgtgggcg aactcctcct cggaggtctc cttgctcacc agggtgtaga
     4501 aggtggagac cctgtccttg ggccgcagga tcctgcccaa ccgttgggcc tcctcctggc
     4561 gggagccgaa ggtcccggag acctggaccg ccacggatgc gtccgggagg tcgatggcga
     4621 agttcgcgac cttcgagacc acgagcacgg gtatgtcgcc cctcctgaac gattcgtaga
     4681 gctcgtcacg gcgggcgttg ctggtcgaac cggtgatcag gggggccttc agcctcgccg
     4741 cgatcctctt gagctggctc aggtactggc cgatcacgag caccggctcg tccatgtgtt
     4801 tcttcaggat ggccgtcaag acacgttcct tgtttccgtt ctcggaggcg atcctgaact
     4861 gcaccctctc gggcgagctc acgtactcgc ggaccttctc cgatgcaagc gggacccgca
     4921 cctcgcagca cagcgcctgg gcgatgaacc ctttttgctc gagctccttc cacggcacgt
     4981 cgtagcactt ggggcccacg agcgagaagg tgtcgtcctc gagcccgtct tccctgatga
     5041 gcgtcgccgt gagccccagc ctccgcttcg cctggagctc ggtggtcgcc ctgaagacgg
     5101 gcgcgggcat gacgtggacc tcgtcataga cgatgagacc ccagttgcgg cccatcatga
     5161 ggttcatgtg ctcgaacggg ccgtctttcg atctccggta ggtcagcatc tggtaggtgg
     5221 tgacggtcac ggcggcgatc tccttcctgc cgcctatgta caccccgatg tccgacggac
     5281 agagcgtggt cttgtccaga agctccgaga cccactggtt caccgcggag acgttggtgc
     5341 acaaaatgag cgtgtgcgtc cgggccctgg ccatcacggc catgcccacc acggtcttgc
     5401 ccgccccgca ggga
//
LOCUS       DAOSCR010000105         5413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9550_length_5413_cov_4.094065, whole genome shotgun sequence.
ACCESSION   DAOSCR010000105 DAOSCR010000000
VERSION     DAOSCR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9550_length_5413_cov_4.094065"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..750)
                     /locus_tag="PLT69_03980"
     CDS             complement(94..750)
                     /locus_tag="PLT69_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ13632.1"
                     /translation="MGVISDNVRSLLAGIPAGVTVVAAAKSRTVAEIEEAIDAGITDI
                     GENYLQEAVPVIGALGDRARWHFIGHVQKNKAKHVVRLFDTIQTVDSVELATLLDAHA
                     SKNGRTVPVLVEVNSGREENKHGVMPEDAVALVRRISTLDNIRVKGLMTMGPFMEDFS
                     GLRPYFRETRMLFEEIASLGIANVAMEVLSMGMSDSYDIAIEEGATMVRIGTRIFGPR
                     "
     gene            1273..2175
                     /locus_tag="PLT69_03985"
     CDS             1273..2175
                     /locus_tag="PLT69_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HOJ13633.1"
                     /translation="MHDKVGYCISRMEILAYKTTFSARSGKGSIPINVSFFPEGQFAN
                     ALKAMRSSFEKGLCVSDLVATAPAGRFLGDIIVPEGSIGFATVCSIVFSGMLLKKGIP
                     LESKFGGILQVSEHKPLRFVDLIHYSGTSLDPSEAFISARMTEVRRAVQHGEGKILAN
                     FSEIAGVCKDGVEEVVSELRDVGMNPVITMGKTGEPICQVPVDVNKMGVILIGGLNPV
                     AAAKEAGVEETTCPCRPSWNTRTSGGSPRSAATFSADAPVPSPQAPSARPAGPGSRPA
                     ATHTPFRLHLHTVWTGDEARAASA"
     gene            complement(2552..4933)
                     /locus_tag="PLT69_03990"
     CDS             complement(2552..4933)
                     /locus_tag="PLT69_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ13634.1"
                     /translation="MKVTGGVYAERGSNQAVPERFERIRRALLSQGERLCTERAVLVT
                     DFFKHHDDPNEPMVVRKAKALRHILQNKSARIFDDELIVGNVGSHRKSVIMQPELAGA
                     VMCYEILWMGRRRNNPYKVPLKDKFRLLAKVIPYWLSRNMVARAFGWYNPRLFRYLAD
                     QLDAKYYLINEGGGIGHFLPGYEAMLKLGIEGFLESLDRDGGDLHAAARIACEGLAAF
                     SARLSEEARRAALKERDLSRREELLEISRICAKVPMKPAGTFHEALQSLWLTHLAVCL
                     EGLNSAVSLGRVDQYLYPFYEADRKAGRMTREKAYELLLCFSAKTTEHMFLLSSGISE
                     YHGGYLVAQAATVGGVDAEGNDAVNDLTWIFLDVMENARLRDPNYMARISVSSDPAYV
                     RRAVEVACRGNAVPGLFSDEAVVKALTAHGYSTAEARDYAIVGCVEPTLPGRSFCSTD
                     AALFNLPMCLLLALNSGRRPGSPFRVGARTPHVRELRSMDDVMGAFKTQVEHMVERLV
                     RDIRVIERANRDFHPTPFSSMLVQGCLESGRDVTAGGARRNSSGIQGVGVADVADSLA
                     AIDEVVFSRRTHSLEEVLEAVLHDFQGYETVRAALASAPKFGNDHGLPERYAGFVVRT
                     FHDALAGYTNTRGGPYIPGFYSSTTHVAFGRRTGALPSGRKAGRPFAASMGCCNGCDR
                     QGPTALLNSVASIDATLCPNGYALNLLIDVGSFSRGEAVDIVGALVQGFFDRKGMEVQ
                     LNVLDPALLAEARRHPGRYPGIVVRVAGYCAYFDELPDSVKDEIIARTAIALG"
BASE COUNT          934 a         1838 c         1708 g          933 t
ORIGIN      
        1 ccatgcaccg atgtatggat gaaggcgcgg cgtcgtgggg gtcaccccgg cccacagcct
       61 gctccccggc ggtgaacccc acggggacgg ccgtcacctg ggcccgaaga tgcgcgtgcc
      121 gatccgcacc atggtcgcgc cctcctcgat ggcgatatcg tacgaatcgc tcatgcccat
      181 ggacaagacc tccatggcca cgttcgcgat cccgagcgag gcgatctcct cgaagagcat
      241 cctcgtctcc ctgaagtagg gcctcaaacc ggagaagtcc tccatgaacg ggcccatggt
      301 catgagcccc ttcaccctga tgttgtccag cgtcgagatc cgtctcacca aggccaccgc
      361 gtcctcgggc atgaccccgt gcttgttttc ctcgcggccg ctgttgacct ccacgagcac
      421 gggcacggtc ctgccgttct tcgatgcgtg cgcgtccagg agggtcgcga gttccacgga
      481 atcgacggtc tggatggtgt cgaagagcct gacgacgtgc ttggccttgt tcttctggac
      541 gtggccgatg aagtgccacc tggccctgtc ccccagtgcg cctatcaccg gcacggcctc
      601 ctggagatag ttctcaccga tgtccgtgat gccggcgtcg atggcctcct ctatctcggc
      661 cacggtccgt gacttggcgg cggcgaccac cgtgacaccc gcggggatac ccgcgagcag
      721 cgacctgaca ttgtcggaga tgacgcccat gaccttgttg tgaggcctca aggcgcctcc
      781 tgtcaaggtt gaacaacaca tacggaaaac gggaggcttc ctccaccgtc tctcgaggat
      841 gccgcctcgg cacccacgcg gacagcgccc gctgtcgaag gccacgaaga ccacacatcg
      901 acgtgcttcc tgcggtcgcg ctcacggcac acacgtgctg aaagacacgg attcaggttg
      961 actgctcgcg cgcgcccacg tatgaggaaa aggaaaccta tttcctctat ggctaggagg
     1021 aacgcacgtg ttcggttacg acgacatgga acgaaagagc gtgttgatcc tgcgcatcct
     1081 caaccaggca cagggtccgc tcggcgcacg gatcatcgcc aggaggctca aggactacgg
     1141 cgtgaacctc tcggagcggg ccgtgcgcta ccacctgcag ttcatggacc agcgggagct
     1201 gacccggttg gtgggcaggc gcgacggcag ggtgataacc gcaaggggca tcgggagctg
     1261 cggaacgccc gcgtgcacga caaggtgggc tactgcatct cgcgcatgga gatcctggcc
     1321 tacaagacca ccttcagcgc aaggtcgggc aagggctcga tcccgatcaa cgtgtcgttc
     1381 ttccccgagg gccagttcgc aaacgcgctc aaggcgatgc ggtcgtcctt cgagaagggc
     1441 ctgtgcgtga gcgatctcgt ggccacggcg ccggcgggcc gcttcctggg cgacatcatc
     1501 gtgcccgagg gcagcatcgg cttcgcgacg gtctgttcca tcgtcttctc gggtatgctc
     1561 ctcaagaagg ggatccccct ggaatccaag ttcggcggca tcctccaggt cagcgagcac
     1621 aagcccttga ggttcgtgga tctcatccac tactcgggca cctccctcga cccgtccgag
     1681 gcgttcataa gcgcccgcat gaccgaggtc aggcgtgcgg tgcaacacgg cgagggcaag
     1741 atcctggcca acttttccga gatcgcaggg gtctgcaagg acggggtgga ggaggtcgtg
     1801 tccgagctca gggacgtcgg catgaacccg gtgatcacca tgggcaagac cggggagccc
     1861 atatgccagg tacccgtgga cgtgaacaag atgggggtga tcctcatcgg cgggttgaac
     1921 cctgtcgccg cggccaagga ggcgggcgtg gaagagacaa cctgcccatg tcgaccctca
     1981 tggaatacaa ggacctcagg aggttctcct cggtctgccg cgactttctc ggctgatgca
     2041 cccgttccct ccccccaggc gccatcggct cgcccagccg ggccgggatc gaggcctgcc
     2101 gcgacccaca caccctttcg cctgcacctc cacaccgtgt ggaccgggga cgaggcccgg
     2161 gcggcttccg cctgaagcgg cctcgggctc ggggatcacc gctcatcacg cacgcagggc
     2221 gcctcggtga ccgcacacgg gggtacgggc atgactcgat cgccggtctc ttgaagccac
     2281 gcctatcaga ccgcatacgg acgggcatgc cgttcgggga cctcattgat ggcgggaaaa
     2341 aatatcaggc cgcacacgga cgggcatgcc gcttccccga agaccccggc ttcacgcatg
     2401 gagggtgggc cgatgaccac gcacggtctc gatgcgcccc gtgtcccggc ccaagacgac
     2461 ttgggaaggc ctcccgtacg ggcgcttcga cgagcggatg cttccagagc gcatgcggca
     2521 gggggtcttg ccgacggcgg gggatgcccg ctcaccctaa cgctatggcg gtcctcgcga
     2581 tgatctcgtc cttgaccgag tcaggcagct cgtcgaagta tgcgcaatac ccggccaccc
     2641 tgaccacgat cccggggtac ctgcccgggt gccgcctcgc ctcggcgaga agcgccgggt
     2701 ccaagacgtt gagctgcacc tccatgcctt tccggtcgaa gaacccttgc acgagcgccc
     2761 ccacgatatc cacggcctcc ccgcgggaga aagagcccac gtcgatgagg aggttcaggg
     2821 catacccgtt ggggcagagc gtcgcatcga tggaagcgac cgagttgagc agggcggtgg
     2881 ggccctgcct gtcgcacccg ttgcagcacc ccatgcttgc cgcaaagggt cgtccggcct
     2941 ttcggccgct cggcagggcc ccggtgcgtc tcccgaaggc cacgtgggtg gtggacgagt
     3001 agaagccggg gatgtagggg cctccccggg tgttcgtgta cccggcgagc gcgtcgtgga
     3061 aggtgcgcac cacgaaaccg gcgtatctct ccggcaggcc gtggtcgttg ccgaacttcg
     3121 gcgccgacgc gagcgccgcc cgtaccgtct cgtacccctg gaaatcgtgc agcacggcct
     3181 cgagcacctc ctcgaggctg tgcgttctcc tggagaagac aacctcgtcg atggcggcga
     3241 gcgagtccgc gacgtccgcg acgccgacgc cctggatacc gctcgagttg cggcgggccc
     3301 cgccggcggt cacgtccctt ccgctctcga ggcaaccctg cacgagcatg gaactgaagg
     3361 gcgtggggtg gaaatccctg ttggcccgct ctatcacgcg gatgtcccgc acgagtctct
     3421 ccaccatgtg ctccacctgg gtcttgaagg cccccatgac gtcgtccatg gacctcaact
     3481 ccctcacgtg cggtgtccgc gcgcccaccc tgaagggaga cccgggcctc ctgccggagt
     3541 tgagcgcaag gagcaggcac atgggcaggt tgaacagggc cgcatcggtc gagcagaagc
     3601 tcctgccggg cagggtcggc tccacgcaac ccacgatcgc gtagtcccgg gcctcagccg
     3661 tggagtagcc gtgggcggtg agagccttta cgacagcctc gtcgctgaac aggccgggga
     3721 ccgcgtttcc cctgcacgcc acctcgaccg cccgtcggac atacgcgggg tccgacgaca
     3781 cgctgatgcg cgccatgtag ttcgggtcgc gcagcctggc gttctccatg acgtcgagga
     3841 agatccaggt gagatcgttg actgcgtcat tgccctcggc gtccacccct cccacggtcg
     3901 ccgcctgggc cacgaggtac ccgccgtggt actcgctgat cccgctcgag agcaggaaca
     3961 tgtgctcggt ggtcttcgcg gagaagcaca ggagcagctc gtaggccttc tccctcgtca
     4021 tgcgccccgc ttttctgtcg gcctcgtaga aggggtagag atactggtct acgcggccca
     4081 ggctgacggc cgagttgagg ccctcgaggc atacggccag gtgggtgagc cagagcgact
     4141 ggagcgcctc gtggaacgtg ccggcaggct tcatgggcac cttcgcacag atcctcgaga
     4201 tctcgagcag ttcctctctt ctcgacaagt ccctctcctt gagcgccgca cgtcgcgcct
     4261 cttccgacag cctggccgaa aacgccgcca ggccctcgca cgctatgcgg gcggccgcat
     4321 gcagatctcc cccgtcgcgg tccagagact cgaggaaccc ctctatgccg agcttgagca
     4381 tggcctcgta gccgggcaga aagtgcccga tgcccccgcc ctcgttgatg aggtagtact
     4441 tcgcgtcgag ctggtctgcg agatacctga aaagcctcgg gttgtaccaa ccgaaggccc
     4501 tcgccaccat gttcctggaa agccagtacg gtatcacctt ggccaggagg cggaacttgt
     4561 ccttgagggg aaccttgtac gggttgttcc gtctcctgcc catccagagg atctcgtaac
     4621 acatgaccgc gcctgcgagc tcgggctgca tgatcaccga cttcctgtgg gaacccacgt
     4681 tgcctacgat gagctcgtcg tcgaagatcc tggccgactt gttctgcagg atatgcctga
     4741 gcgccttggc cttcctgacg accatgggct cgttcgggtc gtcgtggtgt ttgaagaagt
     4801 ccgtgacgag cacggcacgt tcggtgcaca ggcgctcacc ctgggagagc aatgcccgcc
     4861 tgatccgctc gaacctttca ggcaccgcct ggttcgagcc tctttccgcg tatacgcccc
     4921 cggtcacctt cacttgtcac cccccgttcg cgactcccgc acgctgcgcc ctcccgcgcc
     4981 tcccggggcc gaggcggcat cgtgtcggtc aggacaagcc cacgagggtg ggcgggagag
     5041 aaccctcctc cacgtcaccg gggcgccccg agaggaggcg tcctcgtcgc acgccatggc
     5101 gagacccgag agggcccgta tgggcggcag ggtgaaggtc ccccggccga gaaggtcacg
     5161 gctcggccct ctcgactccg tctggtacat gaggaacacg tcacaagcct cgatcggcca
     5221 cctctccatc cccacgcgtc acctccctcg gaaatccccc ggccgctcga tagctatccc
     5281 cgccggtcgg ccgagcatgc gcccgctgat gcacacctca gaactcccgt acgggcgcca
     5341 gcacggtggg ggggtagatc tgccccggca ccgggggcat ggagttgtac acccagtcta
     5401 tgtccacaca acc
//
LOCUS       DAOSCR010000106         5403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9573_length_5403_cov_3.140613, whole genome shotgun sequence.
ACCESSION   DAOSCR010000106 DAOSCR010000000
VERSION     DAOSCR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9573_length_5403_cov_3.140613"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(450..1199)
                     /locus_tag="PLT69_03995"
     CDS             complement(450..1199)
                     /locus_tag="PLT69_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOJ13635.1"
                     /translation="MDKKMKLPGLSRIEKSPDIPTLVMNQIIELISQGKLKIGDKLPS
                     EQEMTKLFGISRISLREAMKLLEAKGYIESRDRKGKYVKSLTDNVKSSIEDILSVDHD
                     KIWELLAVRRLIDSEAAYLAATNVGREHKARFRKIIEVLEKVGDENVLSSKEGGILYS
                     DFFDILADATKNTIFVHLRKTITSVLTGAFPYSRKKLSTVPGSSKAIVGQLLKIHRAI
                     EDRRPDEAKAATIEHIDYIEKSLRKALKEEK"
     gene            complement(1374..2075)
                     /locus_tag="PLT69_04000"
     CDS             complement(1374..2075)
                     /locus_tag="PLT69_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ13636.1"
                     /translation="MRIFAISDLHLSFGSNKPMDVFGAHWEDHPERLREAWLERVTDG
                     DVVLVPGDVSWAMRLAEAADDLAFIHALPGTKVVVKGNHDYWWTTMGKMKNALPPTII
                     PLQNTSVTFGEVAVAGCRLWIDPDLMLEEATEEDRKIFQRELGRLESSLRSVPETAKV
                     RIVMTHFPPISLDGKPSRAVEVASRYGCDIWVFGHMHLGGPDLKGFDRTAGGMRFVFA
                     SADYLGFKPRLIHQD"
     gene            complement(2072..3007)
                     /locus_tag="PLT69_04005"
     CDS             complement(2072..3007)
                     /locus_tag="PLT69_04005"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ13637.1"
                     /translation="MQVVRGLEGIRPETFQRAVVTIGNFDGCHVGHQEIFRRTCAHAG
                     RIGGTGVAVTFDPHPAALVKGITPELIFTLDEKIQAIESLGLDGLVIVPFTREFAETD
                     PEVFVREVVVRRLQARGVVVGHDFAFGKRAAGDIAFLRRMGAKMGFFVECVDPVSRDG
                     QVVSSTLIRRLIHSGDVKRASRLMLHPYRLSGRVVHGMARGRSLGFPTANIVPDKALI
                     PAYGVYAVHAHTGSGSFQGVANIGNNPTFGDTGTSIETFLFGFEGDLYGTVLTIEFVE
                     HIRGEIQFADKRYLVEQIGLDCRRAQEILGGGEQG"
     gene            3081..3167
                     /locus_tag="PLT69_04010"
     tRNA            3081..3167
                     /locus_tag="PLT69_04010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Leu,seq:cag)
     gene            complement(3177..3860)
                     /locus_tag="PLT69_04015"
     CDS             complement(3177..3860)
                     /locus_tag="PLT69_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13638.1"
                     /translation="MDVVVLDDVAFDVEEGALMGLVRLRPQGVRADEFRAVLDEARKV
                     ARPKAACGIAEVGHRGRGRVDIGGVSFTSRLLEENLRDANAAFPYIATCGAELEEWAA
                     GQQGALRQFWAENIMLVALGCAVGYLEGYLKGILGVEGLSSMNPGALDDWPIEQQATL
                     FGLLGDAARSVGCRLAPGMVMRPLKSVSGIYFVSHGGFTNCSLCPRRSCASRRAAFDA
                     ALYARYRGG"
     gene            complement(3863..>5403)
                     /locus_tag="PLT69_04020"
     CDS             complement(3863..>5403)
                     /locus_tag="PLT69_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOJ13639.1"
                     /translation="HRRRHEPMREYTVTLVPSGRSRPFPEGTILSDALVDMGVPLRTP
                     CGGRGLCRRCRVRLGDDASTEVLACRSVVDRDVRVMVAGASTWEVSPFPGLTRGRRLG
                     AAVDLGTTTVKVALVDVDRGRFHTVASFLNPQRRFGHDVVSRIAAAAVLRARDRMTGL
                     IRSALRVCLEKALLASRIDGTTIETVVLSGNTTMLSLFFGLDVSPLGRYPYAAATRDF
                     PGVAPGEVGLDGIVGGEVKGLPVFSAFLGADLVGALTLFGRTSLEGWTLFADLGTNGE
                     LFLAAPSGEVFAASCAMGPALEGMNISCGMSADDGAITRIRSTPAGLAFDMMGTGEPA
                     GITGTALVDLIAVLLDARVIRPDGRLDLDGGRGGLPEGLTAAPEGDMKAVGLWGGLRL
                     TQMDIRNVQLAKAAMHAASRLLMDAAGVAPADVRRVVLAGALGEKMDLEGFMRLGFLP
                     PFGGATFEVAGNTSLEAALTACIDPGFAAQAAQARQRVREVVLSEQEAFQDVFISSLD
                     FPGG"
BASE COUNT          926 a         1866 c         1653 g          958 t
ORIGIN      
        1 actccgaagg acagagacct ggatcaaaag tgcgaaacat tcttgacacc accccatcgt
       61 cgcgccccgg tcttgacggg cgcccatgcc ctgccgatca tggaccggcc tcgagatgcc
      121 ctttccccgt ctgcgtcgat gattacccct gccgcaaggg ctctttgatg tcagtcatct
      181 cgcactttcg tgcggctgag gggtgtgcgg cgatgattgc cccaaccgca agcgctcttg
      241 ccggacaggc atgcctccgg gaggagctga cgaagtggac acggtgatag cgagtccagc
      301 cgcaagcgtt cttgccggac atgcatgcct gcctgagact gcctggccgt ttagcccggc
      361 tgggagggcc ggggcaaagc tcggacaccc cgggcatggt tgcgcacggg atgcgatctc
      421 acgatcttgc ctgaagcgcc tgcgcgctgc tacttctctt ccttgagggc cttccgcagg
      481 gacttctcga tgtagtcgat gtgctcgatc gtggccgcct tcgcctcgtc gggccttcga
      541 tcctcgatcg ccctgtgtat cttcaggagc tggcccacga tggccttcga gctcccgggc
      601 acggtcgaga gcttcttcct gctgtacggg aaggcgcccg tgagcacgga ggtgatcgtc
      661 ttgcgcaggt gcacgaatat ggtgttcttg gtggcgtccg ccaggatgtc gaagaagtct
      721 gagtagagga tgcccccctc cttcgacgag agcacgttct cgtccccgac cttctcgagc
      781 acctcgatga tcttcctgaa cctcgccttg tgctccctcc ctacgttcgt cgcggcgagg
      841 taggcggcct ccgaatcgat gagcctcctc actgccagga gctcccagat cttgtcgtgg
      901 tccacggaga ggatgtcctc tatggaagac ttcacgttgt ccgtgagaga cttgacgtac
      961 ttgcccttgc ggtcgcggga ctcgatgtat cccttggcct cgagcagctt catcgcctcc
     1021 ctgagagaga tcctgctgat accgaagagc ttcgtcatct cctgctcgga ggggagcttg
     1081 tcccctatct tgagcttccc ctgggatatg agctctatga tctggttcat cacaagggtg
     1141 gggatgtcag gcgacttctc gatcctggaa aggccgggca gcttcatctt cttgtccatc
     1201 gtttcccctt cctcccgcgc aggcaagatc atatgctgta gaatatggtg catacacata
     1261 taacgatact acagaaaaac acaagaccga catacgtgga ccatcggcct tcccgggtca
     1321 cggccaagac gtgagggtcc tccgttcgca gggccccttg cctgggcctc gggtcaatcc
     1381 tggtgaatga gcctgggctt gaagccgagg tagtcggcgg acgcgaagac gaacctcatg
     1441 cccccggccg tcctgtcgaa gcccttgagg tcgggcccgc cgagatgcat gtgaccgaac
     1501 acccagatgt cgcagccgta cctggaggcg acctcgacgg ccctcgaggg ctttccgtcg
     1561 agcgagatcg gcgggaagtg ggtcatgacg atacgcacct tcgccgtctc ggggaccgat
     1621 ctcagcgacg actccaacct ccccagctcc ctctggaaga tcttgcggtc ttcctccgtc
     1681 gcctcttcga gcatgagatc ggggtcgatc cagagcctgc aacccgccac ggccacctca
     1741 ccgaaggtga cgctcgtgtt ctgaagcggg atgatcgtgg gcgggagcgc attcttcatc
     1801 ttccccatgg tcgtccacca gtagtcgtgg tttcccttga ccaccacctt ggtgcccggc
     1861 agggcgtgga tgaaggccag gtcgtccgcg gcctcggcca gcctcatggc ccacgagacg
     1921 tctcccggga cgagcaccac gtcgccgtcg gtgacccgtt ccagccaggc ctccctcagg
     1981 cgttcggggt gatcttccca gtgcgcgccg aacacgtcca tgggcttgtt ggagccgaac
     2041 gagaggtgga ggtccgatat ggcgaagatc ctcacccctg ttctcccccg ccgaggatct
     2101 cctgagccct cctgcagtcc aggcctatct gctccacgag gtagcgcttg tcagcgaact
     2161 gtatctcgcc ccgtatgtgc tcgacgaact cgatggtcag cacggtgccg tagaggtctc
     2221 cctcgaagcc gaacaggaag gtttctatcg acgtgcccgt gtccccgaac gtggggttgt
     2281 tgcctatgtt cgccacgcct tggaagctcc ccgaaccggt gtgggcgtgc acggcgtaca
     2341 cgccgtatgc cgggatgagc gccttgtcgg gcacgatgtt ggcggtgggg aagccaaggc
     2401 tcctccccct ggccatgccg tggaccaccc ggcccgagag cctgtagggg tgaagcatca
     2461 gccgggaggc gcgcttcacg tcgccggagt ggatgagcct cctgatgagc gtgctcgaga
     2521 cgacctgtcc gtccctggag accgggtcca cgcactcgac gaagaacccc atcttcgcac
     2581 ccatgcgcct gagaaaggcg atgtcgcctg cagccctctt gccgaacgcg aagtcgtgcc
     2641 ccaccaccac cccacgcgcc tggaggcgcc tcacgaccac ctccctcacg aagacctctg
     2701 ggtccgtctc agcgaactcc ctggtgaacg gcacgatcac gaggccgtcc agccccagcg
     2761 actcgatggc ctggatcttt tcatccaggg tgaagatcag ctccggcgtg atccccttga
     2821 ccagcgccgc ggggtgaggg tcgaacgtga ccgccacgcc cgtaccgccg attcgacctg
     2881 cgtgggcgca ggtcctcctg aagatctcct ggtgcccgac gtgacacccg tcgaaattgc
     2941 cgatggttac caccgcgcgt tggaacgttt cggggcggat cccctcgagg cctctgacaa
     3001 cctgcatgca catgtatcct tgacatttcg taggttccca actatagggg aaacgctgca
     3061 ccaatgcaag ccatgagcac gccgggatgg cggaattggc agacgcgcta ggttcagggt
     3121 ctagtgtgcg tgcgcacgtg cgggttcgag tcccgctccc ggcaccacgt cgagcatcac
     3181 ccgccacgat acctcgcata caaggcggca tcgaaggccg ccctgcgcga agcgcacgac
     3241 ctcctcgggc agagcgaaca gttcgtgaac ccgccgtgag agacgaagta tatgcccgag
     3301 accgacttga gcggcctcat caccatgccc ggggcgagcc ggcaacccac tgatcgcgcc
     3361 gcgtccccca agagcccgaa gagggtcgcc tgttgttcga tgggccagtc atcgagcgcg
     3421 cccgggttca tgctcgagag cccctccacg ccgaggatgc ccttcaagta tccctcgaga
     3481 tagccgaccg cgcacccgag ggccacgagc atgatgttct ccgcccagaa ctggcggagc
     3541 gcgccttgtt gccctgcagc ccactcctcg agctccgccc cgcaggtggc gatgtagggg
     3601 aacgctgcgt ttgcgtccct caggttctct tcgaggagac ggctcgtgaa cgagacgccc
     3661 ccgatatcca cccttccccg cccccggtgc cccacctcgg cgatcccgca ggcagccttg
     3721 ggccgcgcga ccttcctcgc ctcgtcgagc acggccctga actcgtctgc tctcacgccc
     3781 tgcggcctga gccggacgag ccccatgagc gctccttctt ccacgtcgaa cgcgacgtcg
     3841 tcgagaacga ccacgtccac ggtcaccccc ccgggaaatc gagcgacgag atgaacacgt
     3901 cctggaacgc ctcctgctcg gacagcacca cctcgcgcac cctttgcctg gcctgggccg
     3961 cctgggccgc gaaaccggga tcgatgcacg ccgtgagagc ggcttccaag ctcgtgttgc
     4021 cggcgacctc aaaggtggcg ccgccgaagg ggggcaggaa cccgagacgc atgaatcctt
     4081 cgaggtccat cttctctccg agggcgcccg ccagcaccac ccgcctcacg tccgcgggtg
     4141 cgacgccggc cgcatccatg aggagcctcg aggccgcgtg catcgccgcc ttcgcgagct
     4201 ggacgttgcg gatgtccatc tgcgtgagcc tgaggccccc ccacaggccg acggccttca
     4261 tgtccccttc gggcgccgcc gtgagccctt cgggcagacc tccacggcca ccgtcgagat
     4321 ccagccgccc gtccggccgg atgacccggg cgtccaggag cacggcgatc aggtccacga
     4381 gcgccgtgcc cgtaatgcca gcgggctcac ccgtgcccat catgtcgaag gcgaggcctg
     4441 cgggcgtgga ccggatacgc gttatcgcgc cgtcatcggc gctcatgccg cacgagatgt
     4501 tcatgccctc gagcgccggc cccatggcgc aggaggccgc gaagacctcc ccggacgggg
     4561 ccgccaggaa gagttccccg ttcgtgccga gatcggcgaa gagagtccat ccctcgaggc
     4621 ttgtgcgccc gaacagggtg agggcgccga caaggtccgc gccgaggaag gcgctgaaca
     4681 cgggcaggcc tttcacctcg ccccccacga tgccgtccag gcccacctcc cccggtgcca
     4741 cgcccgggaa atccctcgtt gcggcggcgt acgggtacct tcccaggggt gagacgtcga
     4801 ggccgaagaa gagggagagc atggtggtgt tcccggacag cacgaccgtc tcgatcgtgg
     4861 tgccgtcgat gcgggacgca aggagggcct tctccaggca caccctcagc gccgacctga
     4921 tgaggcccgt catccggtcc cgggcgcgca ggacggctgc agccgcaatc cgggagacga
     4981 catcgtgccc gaacctgcgc tgcgggttca ggaaagaggc gacggtatga aagcgccccc
     5041 tgtccacgtc cacgagcgcc accttcaccg tcgtggtccc gagatcgaca gccgcaccca
     5101 gccgcctccc tctcgtgagc cccggaaacg gggagacctc ccaggtggac gcgccggcca
     5161 ccatgacccg cacatccctg tcgacgacgc ttcggcacgc caggacctcc gtggaggcgt
     5221 cgtcaccgag cctcaccctg catctgcggc ataagcccct gccgccgcag ggcgtcctga
     5281 gggggacccc catgtccacg agcgcgtccg agaggatcgt cccctcgggg aagggacggg
     5341 accggcctga agggacaagg gtgaccgtgt actctctcat gggttcatga cgccgccgat
     5401 ggc
//
LOCUS       DAOSCR010000107         5396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9596_length_5396_cov_3.173750, whole genome shotgun sequence.
ACCESSION   DAOSCR010000107 DAOSCR010000000
VERSION     DAOSCR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9596_length_5396_cov_3.173750"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLT69_04025"
     CDS             complement(<1..215)
                     /locus_tag="PLT69_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13640.1"
                     /translation="MSCASDKTEIPTASGLPRARSVPKISRKVPAVLLSALAALFSLA
                     PLNARAEMIDGIAAVVEDTVTGVTAGVA"
     gene            complement(199..3567)
                     /gene="mfd"
                     /locus_tag="PLT69_04030"
     CDS             complement(199..3567)
                     /gene="mfd"
                     /locus_tag="PLT69_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ13641.1"
                     /translation="MAASLVESLGSIDAVGFLPPSLQALVVACTLRARRASVLWLTED
                     TESMERAEDDLLCFLEKGEVHVFPPYDVRPYQDDSPSKEILALRIDALRALAGRAPAV
                     VVAPVSALACHTIAPADLKSRVIEVRPSDVLDRDEFASSLALMGYVREPLVEDVGQFS
                     VRGSVVDVFSPGMESPVRIDLFGDEVSAVKAFDMRTQRTTKALPGATILPAGEIILDQ
                     AAIRRMRPALRRLAGEGAKPLIEDIEHGVHVPGLELFLPLFSDEPACLLDHLAPDATL
                     FVPDDEDVSDAWDRVAEQYSHGHARAASQGRPLPHPQSVLVSKEGLLERMRRSHARVS
                     TTRAFSPDTVRYRSASAYLQAGRGTDAVFDRVLSLITGLGGLYICSSAEMFTERVEYA
                     LTKRGVDVARARGGSILERQGPPTRVTVVPGSLSSGFVLEDLGIAVIGAEELLGAKRR
                     RRRKPSGPPIYDPFTQLDVGDAVVHKDAGIGIFQGVVRLEVDGTSSDFVLLEYLGGDR
                     LYVPMYRLNLLQRYVGDVDRFTVDKLGGTRWIHAKAKASQSVKRLAGELLEMYARRQA
                     AQGFSYDTTNPLVEEFADAFPYTETDDQLKAVEETLSDMAGPRPMDRLICGDVGYGKT
                     EVAMRAAFVAVMSGKQVAVLVPTTLLAHQHLRTFRERMEGWPIRVEVLSSMKGASRRD
                     DVLQEIESGKVDIVIGTHALLSDRVKFKDLGLLVVDEEHRFGVRHKELIKARRAQVDV
                     LTLTATPIPRTLNFAISGLRELSVIETPPAERKAVDTVISRYDEDAVAAAITRELDRG
                     GQVFYVHNQVATIEEAAGRVQTLCPRARVGVAHGQMPRSKLEKVMSAFLDRKITVLVA
                     SAIISSGIDIPNANTIIIDRADKFGLADLYQLRGRVGRSKTKGYCLLLLPEVGQITKD
                     ARKRLAAVKEYESLGAGFHMALRDMEIRGVGDILGRAQWGHVTAIGFELYQEMLKEAV
                     DRLKGVETRPKVEPEIHIGVDAYIPEDYCPDQHLRLGLYRRLFAASAHDLPSIVAELE
                     DLYGPPPEPVTSLLVVSEIRDLMKRLEARKAEVHDGVLRLYVGHDSIVDLERLTSALA
                     RRGGRLRPEGVIEFPLDDARILEETREVLYELCQ"
     gene            complement(3557..4843)
                     /gene="tilS"
                     /locus_tag="PLT69_04035"
     CDS             complement(3557..4843)
                     /gene="tilS"
                     /locus_tag="PLT69_04035"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ13642.1"
                     /translation="MPGPDLLERLLQTLAGPCGVRRGDSVLLCVSGGVDSMVLLHLMQ
                     RARSVMGLDLGVIHVDHGLRKEASREDAAFVQERCLAQGVPFHLASLGMDPSVGDLEQ
                     EARRQRYRAVFECSGAHGYKLVATAHTLDDQAETLLYRAVRGTGVRGLAAMAHRRPDG
                     LIRPLLGVTRAEIARYARENGIAFRLDATNEDVSRPRNLIRREVLPLLERINPGAAPA
                     LASLASIAREEGAVLEEMARSLARQSRVLSGSLVSVHDASMLLSAPLPVLSRLVIELS
                     TSVGGFAQGTSSLQVGLALEVVQGKRRAHVLHRRLRIERSGGLVAAHRVVPPPLYRYR
                     VAAGTGGTVSIPEIGLDVYVGRTVPGDGCVLRSWMEGDRAGGLKATEILASLEVPVLL
                     RPFWPVLESGGSIVAVAAGTPGRGPLLMKGVTGRGR"
     gene            complement(5060..>5396)
                     /locus_tag="PLT69_04040"
     CDS             complement(5060..>5396)
                     /locus_tag="PLT69_04040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ13643.1"
                     /translation="GAACEFAVMVHPCWQCKGLGRHLLGCMVEIARRLGFKTLRMHVW
                     EENERMLKMLSGFGFHMNAFVEYNVIRMDFDLEGVPEGVADLLPGETCSMPAGPLSSP
                     GASAIHRIN"
BASE COUNT          872 a         1863 c         1746 g          915 t
ORIGIN      
        1 gccacccccg ccgtgacgcc tgtgaccgtg tcttccacca cggccgctat cccgtcgatc
       61 atctccgccc gggcgttcaa cggggcgagc gagaagagcg ccgcgagcgc ggagagaagc
      121 accgcgggaa ccttccggct gatcttcggc acagaccgtg cacggggcag cccagaggcg
      181 gtcgggattt cggtcttgtc actggcacag ctcatagagc acttccctcg tttcctcgag
      241 gatccttgcg tcgtcgagcg ggaactcgat cacgccctcg ggcctgagcc gcccacccct
      301 cctcgcgagg gcggacgtga gcctctcgag gtcaacgatg ctatcatgcc cgacgtacaa
      361 cctcaacact ccgtcatgta cctcggcctt gcgcgcctcg agccttttca tgaggtcgcg
      421 gatctccgag acgaccagca gcgatgtcac cggttccggg ggcgggccgt agaggtcctc
      481 gagctcggcc acgatcgacg gcaggtcgtg ggcggatgcg gcgaagagcc tccggtagag
      541 gcccagcctc aggtgctggt cggggcagta gtcttcgggt atgtacgcgt ccacgccgat
      601 atggatctcg ggctcgacct tcggcctcgt ctcgacgccc ttgagcctgt cgaccgcctc
      661 cttgagcatc tcctggtaga gttcgaaccc gatggcggtg acgtgccccc actgcgccct
      721 gccgaggatg tcccccaccc cccggatttc catgtctcga agcgccatgt gaaaacccgc
      781 gcccagcgac tcgtactcct tcaccgctgc gagccgcttg cgtgcatcct tggtgatctg
      841 cccgacctcg ggcagcaaga ggaggcagta acccttggtc ttggacctcc ccacccttcc
      901 cctgagctgg tacaggtccg caaggccgaa cttgtccgcc ctgtcgatga tgatggtgtt
      961 ggcgttcggg atgtcgatgc ccgagcttat gatggccgat gcgacgagca cggtgatctt
     1021 cctgtccagg aaggcggaca tgaccttttc cagcttcgac ctcggcatct ggccatgggc
     1081 gacccccacc ctcgcgcggg ggcacagcgt ctggacgcga cccgccgcct cctcgatggt
     1141 ggccacctgg ttgtgcacgt agaagacctg gccccccctg tcgagctcgc gggtgatggc
     1201 agcggccacg gcgtcctcgt cgtaacggct gatcacggtg tccacggcct tccgttccgc
     1261 aggcggcgtc tcgatgacgg agagctcccg caggcccgat atggcgaagt tgagggtcct
     1321 cgggatgggg gtggcggtca gggtcaggac gtcgacctga gccctccttg ccttgatgag
     1381 ctccttgtgc cgcacgccga accggtgctc ctcgtcgacg acgaggagac cgaggtcctt
     1441 gaacttcacc ctgtccgaga ggagggcatg cgtcccgatg acgatgtcga ccttgcccga
     1501 ttcgatctcc tggagcacgt cgtctctgcg ggacgcgccc ttcatgctcg acaggacctc
     1561 gaccctgatg ggccagccct ccatccgttc cctgaacgtc ctcaggtgct ggtgggccag
     1621 gagggtggta ggcaccagga cggcgacctg cttgccgctc atgaccgcca cgaaggcggc
     1681 gcgcatggcc acctcggtct tcccgtaccc cacgtccccg cagatgagcc tgtccatggg
     1741 ccgtggcccg gccatgtccg agagggtctc ttccaccgcc ttgagctggt cgtccgtctc
     1801 cgtgtagggg aaggcgtcgg cgaactcttc caccaggggg ttcgtcgtgt cgtaggagaa
     1861 gccctgggcg gcctgcctcc tggcgtacat ctccagcagt tcccccgcga gcctcttcac
     1921 cgactggctg gccttcgcct tggcgtgtat ccacctggta ccgccgagct tgtcgacggt
     1981 gaagcgatcg acgtcgccca cgtagcgctg gaggaggttc agcctgtaca tggggacgta
     2041 gaggcggtcg cctcccaggt actcgaggag cacgaagtcg ctcgacgtgc cgtcgacctc
     2101 caggcgcacc accccctgga agatcccgat accggcgtcc ttgtgaacca ccgcgtcccc
     2161 gacgtcgagc tgggtgaacg ggtcgtagat cggggggccc gagggtttcc tcctcctcct
     2221 tcgcttcgcg ccgaggagct cttctgcgcc tatcactgcg atcccgaggt cttcgagcac
     2281 gaaccccgac gacaggctgc ccggcacgac cgtgaccctg gtgggcgggc cttggcgctc
     2341 gaggatcgaa ccgccccggg cacgcgcgac gtcgaccccc cgcttggtca gggcgtactc
     2401 caccctctct gtgaacatct ctgcggaaga gcagatgtac aggccgccaa ggccggtgat
     2461 caacgacaac accctgtcga acacggcgtc cgtgcccctg cccgcctgga ggtacgccga
     2521 ggcggaccgg tacctgacgg tatccggtga aaaggccctg gtcgtggaga ccctcgcgtg
     2581 agagcgccgc atgcgttcca ggagcccttc cttcgagacg agcacgctct gtggatgcgg
     2641 gagcggcctt ccctgcgagg cggcccgggc gtggccgtgg gagtactgtt cggcgaccct
     2701 gtcccatgca tcggacacat cctcgtcgtc gggcacgaag agggtggcgt ccggggcgag
     2761 gtggtcgagc aggcaggcgg gttcgtcgga gaagaggggg aggaagagct cgaggccggg
     2821 gacgtgcacg ccgtgctcga tgtcctcgat gaggggtttc gcgccttccc cggccagcct
     2881 cctcaaggcg ggcctcatac gcctgatggc ggcctggtcg aggatgatct ctcctgcagg
     2941 caggatcgtc gcacccggca gggccttcgt ggtcctctgc gtcctcatgt cgaacgcctt
     3001 gaccgcactg acctcgtcgc cgaacaagtc tatgcgcacc gggctttcca tgcccggcga
     3061 gaagacgtcc acgaccgagc ccctcacgct gaactggccg acgtcctcca cgaggggctc
     3121 ccggacgtat cccatgagcg cgaggctcga tgcgaactca tccctgtcga ggacgtccga
     3181 gggcctcacc tcgatgacgc gggacttcag atcggcaggg gcgatggtgt gacacgcaag
     3241 cgcggatacc ggggcgacga cgacggccgg cgcacgccct gcaagcgccc tgagcgcatc
     3301 gatcctcaag gcgagtatct ccttcgaggg gctgtcgtcc tggtagggcc tcacgtcgta
     3361 gggcgggaag acatgcacct cccccttctc gaggaagcac aagaggtcgt cctcggccct
     3421 ctccatggat tccgtgtcct cggtcagcca gagaacgctc gcgcgcctgg ccctgagcgt
     3481 gcaggcgacc acgagcgcct gcagggaggg tgggaggaac cccaccgcat cgatcgaccc
     3541 gaggctctcg acgaggctag cggccacgac ctgtcacccc tttcatgagg agcggccccc
     3601 gtccgggcgt cccggctgca acggcgacga tgctcccccc gctctcgaga accggccaga
     3661 agggtctcaa cagcaccggg acttccagcg aggcgaggat ttcggtcgcc ttgagtcctc
     3721 cggccctgtc cccttccatc catgacctga gcacacaccc gtcgccgggg acggtccgac
     3781 ccacgtacac gtcgagaccg atctcgggta tcgagaccgt gccgcctgtc cccgctgcca
     3841 cgcggtaccg gtagaggggg ggaggcacga cacggtgagc cgccacgagc ccgcccgaac
     3901 gctcgatcct gagcctgcgg tgaaggacgt gggcacgtct ctttccctgc acaacttcca
     3961 gcgccaggcc cacctgcagc gaggacgtcc cctgggcgaa cccacccacg gaggtggaga
     4021 gctcgatgac caggcggctg agcaccggca gaggggcgga aaggagcatc gaggcgtcgt
     4081 gcaccgagac caggcttccc gagaggacgc gggactgcct cgcgagcgac cgcgccatct
     4141 cttcgagcac ggccccttcc tcccgggcga tggatgcgag cgaggccagg gccggggccg
     4201 ccccggggtt gatacgctca agcaggggca gaacctctct ccggatgagg ttcctgggcc
     4261 tcgacacatc ctcgttcgtt gcatccagcc tgaaggctat gccgttctca cgggcgtacc
     4321 gcgcgatctc ggccctcgtc acgccgagga ggggacggat gaggccgtca ggcctcctgt
     4381 gcgccatggc agcgagcccc ctcaccccgg tgccgcgcac ggcgcggtac aggagcgtct
     4441 ccgcctggtc gtccagggtg tgggcggtgg cgacgagctt gtacccatgg gcgcctgagc
     4501 actcgaacac ggcgcggtac ctctgacgcc tggcttcctg ctcgagatcg cccacggagg
     4561 gatccatgcc gaggctggcg aggtggaagg gtacgccctg agccagacag cgctcctgca
     4621 cgaaggccgc gtcctcgcgg gacgcctctt tcctgagccc gtggtcgacg tggatcaccc
     4681 cgagatcgag gcccatgaca ctccgggccc gctgcatgag gtgcaagagc accatggaat
     4741 cgacccctcc cgacacgcac aggaggacgg aatctccccg cctcacgccg cacgggccgg
     4801 cgagtgtttg caggaggcgc tcgagcagat ccggacctgg catggcacca tcattattcc
     4861 ctgaggcacg ccacggtcaa gaccgcgccc cgccgggggc cgtatccgca tgcatggccg
     4921 gctcaccggt cttccccggt cccggcgcgc tccccgaggg actgggcggc tcccttaaga
     4981 ccgccctcgc accgggtgtg cagactaggg tcggggtgcg catgacagga gaagggtcgt
     5041 tcctgcgggc tggcggtgtc tagttgattc gatggatcgc gcttgcgccc ggcgaggaga
     5101 gtggacccgc cggcatcgag cacgtctcgc ccggaagaag atctgcgacc ccctccggta
     5161 ccccctcgag gtcgaagtcc atgcgtatca cgttgtactc tacgaaggca ttcatgtgaa
     5221 agccgaagcc ggacagcatc ttgagcatcc gctcgttctc ctcccagacg tgcatcctca
     5281 gcgtcttgaa tccgagccgc ctggctatct cgaccatgca cccgagaagg tgccgcccga
     5341 gacctttgca ctgccagcag ggatggacca tgacggcgaa ctcgcaggcg gcccct
//
LOCUS       DAOSCR010000108         5384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9630_length_5384_cov_2.566898, whole genome shotgun sequence.
ACCESSION   DAOSCR010000108 DAOSCR010000000
VERSION     DAOSCR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9630_length_5384_cov_2.566898"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5371)
                     /locus_tag="PLT69_04045"
     CDS             complement(<1..5371)
                     /locus_tag="PLT69_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOJ13644.1"
                     /translation="MSLVVQPPVIAQGERAHLSWFSENAQSCHIDNGIGQVGVNGGVD
                     VAPLGTTTYTITAVGLGGTAKASATLEVLPRPTVVITADPNPIDRGTATVLTWRSTDA
                     DRAVIDQGIGEVETEGRLEVRPDATTTYTITATGPGGTAEASVTVEVRGAGKSRPSCA
                     FIANNGSTTVSVIDLQTRTVTAAIETGQAPYGIAVSPDGDRAYVTTELDGVFVIDVAT
                     NRVTSIIPVRAATVATSPDGSLVYTVSPDEGMLRAFRANTHEQVGSVPIDMSPRSIAV
                     SPDGSKIYVGCLDREVIHVVDAARMARAGTIEAGEGLGSVCDLETSPDGSRLYAISNS
                     SCRLMVIDTKTDTIVDERYYLVERKPDEAYLAVSPDGAKIFMSFILGMDNAVFSIDAG
                     TLERTAAIEASMPSDMSFTPDGSLLVFPDAREEGVFIVDAARDRVEAGLQGGLTFPYT
                     CGHFVAEKRWRVQGKVVCRGTGVGGVRVAITDGRVTRSAVTDQAGVYTLYVPAGSYTL
                     SFEEAGFLFRPPSLSVSVEDRNVTLDDVEVLLGARITATPSEIAEGGVSTLSWETFEA
                     STVRVEPGIGEVSRSGSMEVSPSASTVYTITAGNAAGYAVTAETSVQVHRRPSVNITV
                     DPCRIACGQSTRVSWTSSYADKVSILPYGWDMEPEGSFVDTPQATTTYEAVASGPGGV
                     ARAQAVVTVLGKPAIVLSVDRPSIWLGEQAVLAWEVSDADEVSISSGIGAVQASGSLV
                     VSPTETTTYTLTATGPGGQSSSSVTLTVLACSVRGVVRDGQTGRALQGVRVTVSGSET
                     TVSTVTDASGSFHASGRICGEVTITCEMAGYETREGSYLCSLGSELTILLTLVPLGGL
                     GVEVISPVNGAVVTVSPVVVRGTVSSPARVQVNGIDAVVAGLTFEALVPLSEGENTVT
                     VVATDESGRHATAELTVTLRPGADRARLEGTVMDSASGWAIPGATVTVTDGEGQKGAT
                     SAPDGSFAVADIAPGAVEVRCERQGYLGNSFTDVLSAGETLRVTIRMEPRSDMAGIKG
                     RVLDAATGEPLAGAIIDAEAGGARASALSGPDGAYMVAGLSCGLLTVNVSMEGYLGQK
                     LSGELATPGMYTLDIALHALSSRATVFGEAVNASTGLFEEGVRVTHVGTGITAMSGPD
                     GRFSLTGIPVGPQVFEVKKAGFVDKEYRVDITSLTFRLDIMEPLRQAASHPRVTGPNL
                     SGKVVDALSGKPLPGAVVKVMNTDLQVTADANGRYSFANLPYGEQFLVAYVHDHEAVS
                     MNPKVAPGMSGDLDFRLPPTTVGVIAGRVTDARTGLPIKNVRVRLWGSALLSALTEHD
                     GTYKIMSVPVGVHDLSFVHPMYREGARPAVRVEAEQTTVVDVALVGRPLTGSLTGTVR
                     DEDTLAPLAGARVSACGKAVTADSQGRYTLSGLPSGLVGIQVEADGYHTSHVSAAVDA
                     DRDDATPTIRHVDLFVSALRQTKMHPVSEYIVSARGGYVTSADRQLTVVFPPGCLSAD
                     AVVTIMPAEGGSQVQPGGTLDLDPGLGLTGVRALGDVLRLVIEPAHPQDPIPSVNSWV
                     VVAARFSQELVDAHGIDEYTVMPYCHDGLHWSLPRVMPHEKMLEPVNNMVVCTVALSS
                     SITGAPLSERLGTKQPVLLAQLDGHVPDLDLARIFTLVLAGLSNLGAAPSPNVRIYDN
                     KAGDVVGGQASKLSMVPEDPRNEKCPNPNALPLLVFHGWDPLAAIMNVDGVNPNNPDS
                     RYYHILDDLVKMTNGVYRPVFLSYNTRCGIEDIGNEVAKRLHPSGSDLFKGMPVPGRE
                     GTTGIF"
BASE COUNT          859 a         1870 c         1691 g          964 t
ORIGIN      
        1 ggaagatgcc ggtcgtccct tcccgcccgg ggacgggcat acccttgaac aggtcggaac
       61 ccgaggggtg gagcctcttc gccacctcgt tcccgatgtc ctcgatgccg caccgggtgt
      121 tgtacgagag aaatacggga cggtatacgc cgttggtcat cttcacgaga tcatccagga
      181 tgtgataata tcgtgaatct gggttattcg gattcacccc gtccacgttc atgatggccg
      241 cgagcgggtc ccacccgtgg aacacgagca ggggcagtgc gttcgggttc gggcacttct
      301 cgttcctcgg gtcctcgggc accatggaga gtttggaggc ctggccgccc accacgtcgc
      361 ccgccttgtt gtcgtagatc cggacattcg gcgacggcgc cgcacccagg tttgaaaggc
      421 cggcgagtac gagggtgaag atccgggcga ggtcgaggtc aggcacgtga ccgtcgagct
      481 gggccaacag cacgggctgt ttcgtgccga gccgctctga caggggcgca cccgtgatgc
      541 tcgaagagag cgccacggtg cagacgacca tgttgttcac cggctcgagc atcttctcgt
      601 gcggcatcac gcgcggcagg ctccagtgca ggccgtcgtg gcagtacggc atgaccgtgt
      661 actcgtcgat accgtgggcg tcgaccagtt cctgggagaa ccgcgcagcc accacgaccc
      721 acgagttcac gctcgggatc gggtcctggg ggtgtgcagg ttcgatgacc agcctcagta
      781 cgtcaccgag cgccctgacc ccggtgaggc cgagaccggg gtcgagatcc aaggtccccc
      841 cgggttgcac ctgggagcca ccctcggccg gcatgatcgt gaccaccgcg tccgcgctca
      901 ggcacccggg cggaaacacg acggtcaact gcctgtccgc cgaggtgacg tatccgcccc
      961 tggcggagac gatgtactcg gagacggggt gcattttcgt ctgcctgaga gcgctcacga
     1021 agaggtccac gtgcctgatg gtgggcgtcg catcgtcgcg gtcggcgtcg acagcggcgc
     1081 tgacatgcga cgtgtggtac ccgtcggcct ccacctgtat gcccaccagg cccgaaggga
     1141 ggccgctcaa cgtatacctg ccctgcgaat cggctgtcac ggccttcccg cacgcagaga
     1201 ccctcgcacc ggcaaggggg gcgagtgtgt cctcgtcacg gaccgtaccc gtaagcgagc
     1261 ccgtcaaggg cctgcccacg agggccacgt ccacaactgt cgtctgttcc gcctccaccc
     1321 tgacggccgg cctcgccccc tccctgtaca tcgggtgcac gaaggaaagg tcgtgcacgc
     1381 ccacgggcac gctcatgatc ttgtaagtgc cgtcgtgctc ggtgagcgcg gacagcagcg
     1441 cgcttcccca cagccgcacc cggacgttct tgatcggcag cccggtacgg gcgtcggtca
     1501 ccctgccggc tatgacaccc acggtcgtgg gcggcagcct gaagtcgagg tctcctgaca
     1561 tccccggcgc caccttcggg ttcatggaga ctgcctcgtg gtcgtgcacg taagcgacca
     1621 ggaactgctc gccgtagggg aggttcgcaa acgagtaccg tccgtttgca tctgccgtga
     1681 cctggaggtc cgtgttcatg accttcacga ccgctcctgg cagcggtttc ccgctgaggg
     1741 cgtcaaccac ctttcccgag aggttcgggc ctgtcaccct ggggtgcgaa gccgcctgcc
     1801 tcaggggttc catgatgtcg agcctgaacg tgaggctcgt tatatcgacc cggtattcct
     1861 tgtcgacgaa ccccgccttc tttacctcga acacctgggg ccccacgggg atccccgtca
     1921 aggagaacct gccgtcaggc ccgctcatgg ccgtgatccc ggtgcccaca tgcgtgaccc
     1981 tgaccccctc ctcgaaaagc ccggtggagg cgttgacagc ctcgccgaac accgtcgcgc
     2041 gcgaggacag tgcgtgcagg gcgatatcca gcgtgtacat ccccggtgtc gcgagctcgc
     2101 ccgagagctt ttggccaagg tagccctcca tgctcacgtt cacggtgagc aagccgcagg
     2161 aaaggcctgc caccatgtac gccccgtccg gaccgctcag cgcggatgcc ctcgcgccgc
     2221 cggcctcggc atcgatgata gcccctgcga ggggctcacc ggttgcggcg tcgagcacgc
     2281 gacccttgat gccggccatg tctgaccgtg gctccatgcg aatggtcacc ctcaaggttt
     2341 cacccgccga gaggacatcc gtgaagctgt ttcccaggta cccctgcctt tcgcacctca
     2401 cctccaccgc cccaggtgcg atgtcggcca cggcgaacga cccgtccggc gcgctcgttg
     2461 cccccttctg cccctcgccg tctgtgaccg tgaccgtggc gcccggtatg gcccagcccg
     2521 aggccgagtc catgaccgtg ccctcgagcc tcgccctgtc ggcccccggc cgcaaggtta
     2581 ccgtcaactc cgccgtggca tgcctgccgc tctcgtcggt cgcgacgacc gtgacggtgt
     2641 tctccccttc cgaaagcggc accagggcct cgaacgtgag gcccgcaacc accgcgtcta
     2701 tgccgtttac ctgcacccgt gcaggggagc tcacggttcc ccgcaccacg acagggctta
     2761 cggtcacgac agcgccgttt acgggtgaga tcacctcgac accgagcccg ccgagtggca
     2821 cgagggtcag caagatggtg agctcgctcc ccagggaaca gaggtacgac ccctccctcg
     2881 tctcgtaccc ggccatctcg cacgttatgg tcacctcgcc gcagatacgg ccgcttgcgt
     2941 gaaaagaccc cgaagcgtcg gtcacggtgg agacggtggt ctccgaccca ctgaccgtca
     3001 ccctgacccc ttggagcgcc ctgcctgtct gtccgtctct caccactccc ctgacgctgc
     3061 acgcaaggac cgtgagggta accgaagagc tcgactgtcc gccagggccg gtcgcggtga
     3121 gggtgtaggt ggtcgtttcc gtggggctga cgacgagact cccgctcgcc tggacggcgc
     3181 ctatgccgct ggagatcgac acctcgtcgg catcgctcac ctcccaggcc agtacggcct
     3241 gttccccaag ccagatcgag ggcctgtcca cggagaggac gatggccggc ttccctagca
     3301 cggtgacgac cgcctgcgcc ctcgcgaccc cgccggggcc gcttgcgacg gcctcgtagg
     3361 tggtcgtggc ctgcggcgtg tcgacgaacg agccctcagg ctccatgtcc cacccgtagg
     3421 ggaggatgct caccttgtcc gcgtagctgc tcgtccagct cacccgtgtg gactggccgc
     3481 aggctatgcg gcaggggtcg accgtgatgt tcacgctcgg cctccggtga acctggaccg
     3541 atgtctcagc cgtgacagca taaccggccg cgttgcccgc cgtgatggtg tacaccgtgc
     3601 tcgcagaagg cgagacctcc atggaacctg accgggagac ttcgccgatg cccggctcga
     3661 ccctgacggt tgaggcttcg aacgtctccc acgagagcgt cgagacgccg ccttcggcta
     3721 tctcgctggg ggtggcagtg atcctggcac cgaggagcac ctccacgtca tcgagggtga
     3781 catttctgtc ctccacgctc acgctcaaag agggaggcct gaacagaaag cccgcctcct
     3841 cgaacgaaag ggtgtagctt cctgcaggga cgtacagggt gtacacgccc gcctggtccg
     3901 tgaccgccga cctcgtgacc ctgccgtcag tgatcgccac cctcaccccg ccgacgcccg
     3961 ttcctctgca caccactttt ccctggacgc gccagcgctt ttccgcgacg aagtgcccgc
     4021 aggtgtaggg gaaggtcagg ccgccttgca gccccgcctc gaccctgtcc cgggcggcgt
     4081 caacgatgaa caccccctcc tcccgcgcat ccgggaagac caggagcgac ccatcgggcg
     4141 tgaagctcat gtcggaaggc atggacgcct ctatggccgc cgttctctcg agggtgcccg
     4201 cgtcgatgga aaagaccgcg ttgtccatgc ccaggatgaa gctcatgaag atcttcgcgc
     4261 cgtcggggct cacggcgagg tatgcttcat cgggcttgcg ctcgacaagg taatacctct
     4321 cgtccacgat ggtgtccgtc ttggtgtcga tcaccatgag cctgcacgag ctgttggata
     4381 tcgcgtacag gcgagacccg tccgggctcg tctccaggtc gcagacggac ccaaggcctt
     4441 cgcccgcctc gatggttccc gctctcgcca tgcgggcggc atccaccacg tgaatgacct
     4501 cgcgatcgag gcaccccacg tatatctttg acccatcggg actgacggcg atcgacctcg
     4561 gcgacatgtc gataggcaca gagcctacct gctcgtgggt gttcgccctg aaagccctca
     4621 gcatgccctc gtctggcgag accgtgtaga cgagggagcc gtccgggctc gtcgcaacgg
     4681 tcgcggcgcg tacggggatg atggaagtca ccctgttcgt ggcgacgtcg atcacgaaca
     4741 cgccgtcgag ctccgtggtg acgtacgcgc ggtctccgtc cgggcttacg gcgatcccgt
     4801 acggcgcctg gcccgtctct atggcggccg ttaccgtgcg cgtctgcaag tcgataacgc
     4861 tcacggtggt gctgccgttg ttggcgatga aggcgcacga gggcctgctc ttgcctgcgc
     4921 ctctcacctc caccgtgacc gatgcctcgg ccgttccgcc gggccccgtt gccgtgatcg
     4981 tgtacgtggt cgtcgcatcg ggcctcacct cgagtcggcc ttccgtctcg acctcgccta
     5041 tgccctggtc gatgaccgcc ctgtccgcgt ccgtggacct ccaggtaagg acagtggccg
     5101 tccccctgtc gatcgggttc ggatccgcag tgatcacgac cgtgggcctc gggaggactt
     5161 cgagcgtggc ggacgccttg gccgtgccgc ctaagcccac ggcggttatg gtgtaggtcg
     5221 tggtcccaag gggtgcgaca tcgacgccgc cgttcacccc gacctgcccg atgccgttgt
     5281 cgatgtggca cgactgcgcg ttctcggaga accagctcag gtgggcgcgc tcaccctggg
     5341 cgatcaccgg cggctgcaca accaggctca cgacgggcgg ttcg
//
LOCUS       DAOSCR010000109         5362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9676_length_5362_cov_3.041078, whole genome shotgun sequence.
ACCESSION   DAOSCR010000109 DAOSCR010000000
VERSION     DAOSCR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9676_length_5362_cov_3.041078"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLT69_04050"
     CDS             <1..361
                     /locus_tag="PLT69_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOJ13645.1"
                     /translation="IGLSPPPMQTDKGWLVLYHGVRATASGCIYRLGLALLDRQDPTR
                     VLMRSDEWVFGPQEDYEVVGDVDKVVFPCGWVRHGDEVRLYYGAADRCIGLAFTTVGK
                     LLSWLEEHHDRDRRAWP"
     gene            361..1377
                     /locus_tag="PLT69_04055"
     CDS             361..1377
                     /locus_tag="PLT69_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ13646.1"
                     /translation="MRVAVLSPIAWRTPPRHYGPWEQVASLLAEGLVRRGFDVTLFAT
                     GDSVTSARLVSVCPRGYEEDKTLIPKVWEALHIAALFERAHEFDLIHNHFDFLPLTYL
                     CATKTPVLTTIHGFSSEGILPVYRAYNGRAHYVSISNADRREGLDYLATVYHGIDLGQ
                     FTFRQAHGGYLLFFGRIHHDKGARECIEAASRAGMKLVMAGIVQDREYFERHVKPHVD
                     GRRVVFVGSADPRTRDELLGKAYALLHPINFDEPFGLSVVEAMACGTPVIAFNRGSMP
                     EIVRHGVTGFLVRDVDAMVDAVPRVRDLDRAQARRWVEERFSAERMVDDYARLYELIV
                     SRGR"
     gene            1381..2325
                     /locus_tag="PLT69_04060"
     CDS             1381..2325
                     /locus_tag="PLT69_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOJ13647.1"
                     /translation="MGKERRSIVERYPGNPILTKDDVPYPVETVHNAAVVKHAGGYTM
                     LFRSHLKNGRSVIGIADSRDGYAFTVRDRPFVTPATEGPFAQVEEFGVEDPRITLVEG
                     EYLVTYSAYSRFGVRVVLARTADFEAVERIAVITQADCRNVVLFPERIGGRYVRLDRP
                     HSEIMPWSIWMSSSPDLVHWGESKPLISPMTYHWDEMKVGPGSPPVKTERGWLHVFHG
                     VFKTMDGAVYRLGTALHDLADPSRVIGVADEWILQPEDPWEVTGYVHNVVFCCAMVPE
                     DDRTAKIYWGGADTVVCAGTVDMEELVRLCIDRPRPPL"
     gene            2388..2795
                     /gene="tsaA"
                     /locus_tag="PLT69_04065"
     CDS             2388..2795
                     /gene="tsaA"
                     /locus_tag="PLT69_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOJ13648.1"
                     /translation="MEISFKPIGVMHSPLKDAETAPRFYTESDVKGVIEVYPEYEAGL
                     SNLSDYTHIVVVFYFHKSRGYELLQRRRGVGELRGVFSLCSPNRPNPIGISVLRLLLV
                     EGNMLHVDNVDILDDTPILDIKPYKPFDYPQGG"
     gene            3046..3924
                     /locus_tag="PLT69_04070"
     CDS             3046..3924
                     /locus_tag="PLT69_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13649.1"
                     /translation="MEYDMPLFRPPSEARSLIFQVTLGCSWNRCVFCAMYKTKKFRVR
                     PFEEVEKDVIEMSWLYPGTEKIFLADGDPLAAPTGYLIDVLDLMNQRFPNLKRISAYA
                     GPTNLLEKSVDELRELRKRKLDVLYLGIETGNDALLKRIRKGATAAGIIQGCRKAVEA
                     GLRMSTFIILGLGGVDGSYVHAKDSARVANAIDPHFLATLTLMLGPYERVYEEKIMGG
                     GFKLIDKRQSLQELRWFVEDLEVSDCKFGSEHASNYLPISGYLPRDKEEILRLIDTAL
                     SDASSGLLRPEWARGL"
     gene            3924..5198
                     /gene="hisS"
                     /locus_tag="PLT69_04075"
     CDS             3924..5198
                     /gene="hisS"
                     /locus_tag="PLT69_04075"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOJ13650.1"
                     /translation="MDIRAVRGMNDILPEEAVLWRFVEEEARRIFALYGFEELRPPLL
                     ERTELFLRGIGDETDVVEKQMYTFTDKGGDSVTLRPEATACVLRAVIEHGILNKDPVA
                     KLYTIGPMFRYERPQKGRFRQFHQINAERLGEDGPLADAETLAMAYDIVRSVLGGEGL
                     VMEVNSLGCKQCRPAFTEALKAFLAAREDLLCSDCRRRMVSNPLRVLDCKVPTCKEAV
                     MDAPTLDATLCGGCREHHECVKEGLDALGVRYEQNVHLVRGLDYYTRTTFEIVSASLG
                     SNSAVAGGGRYDDLLGLLGGPPVGGIGFAIGMERLVMLAPEGLAKKGEGCFVAAQNTH
                     EARLEALRLMMELRGNGIPADSALGRSFKAQMRRADKGGHPVCVIVGEEELRTGTVSL
                     RDMRTGSQETVPRADLVSRILSVLDKGKGEKA"
     gene            5216..>5362
                     /locus_tag="PLT69_04080"
     CDS             5216..>5362
                     /locus_tag="PLT69_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOJ13651.1"
                     /translation="MARTHYCGEIGDAPEGSRVTLYGWVQRRRDLGGLIFIDLRDRTG
                     IAQVV"
BASE COUNT          992 a         1610 c         1869 g          891 t
ORIGIN      
        1 gatcggcctc agcccccctc ccatgcagac ggacaagggg tggctcgtgc tctaccacgg
       61 ggtgcgcgcc acggccagcg ggtgcatcta ccggctgggg ctcgcgctgc tcgaccggca
      121 ggatccgacc agggtgctca tgaggtccga cgagtgggtg ttcgggcctc aggaggatta
      181 cgaggtggtc ggcgacgtgg acaaggtggt tttcccctgc gggtgggtga ggcacggcga
      241 cgaggtgagg ctctactacg gggcggcgga tcgatgcatc ggtctcgcct tcacgacggt
      301 gggcaagctg ctttcgtggc tcgaggagca ccacgaccgc gacaggaggg cctggccgtg
      361 atgagggtgg ccgtgctctc gcccatagcg tggcggacgc cccccaggca ttacggtccc
      421 tgggagcagg tggcgtcgct ccttgccgag gggctcgtca ggcgaggctt cgacgtgacc
      481 ctcttcgcca caggcgattc cgttacgagc gcgaggctcg tgtccgtgtg tccccgcggc
      541 tacgaggagg acaagacgct catccccaag gtctgggagg cccttcacat agcggccctc
      601 ttcgagcgcg cacatgagtt cgacctgata cacaaccact tcgacttcct ccccctgacg
      661 tacctgtgcg ccacgaagac cccggtgctc accaccatac acgggttctc ctcggaaggg
      721 attcttccgg tctaccgggc gtacaacgga agggctcact acgtgtccat cagcaatgcc
      781 gaccgcaggg aggggctcga ttacctcgcg accgtctatc acggtatcga tctcggccag
      841 ttcaccttca ggcaggctca cggcgggtac ctcctcttct tcggcaggat ccaccacgac
      901 aagggcgcgc gggaatgcat cgaagcggcc tcgagggcgg gcatgaagct cgtgatggcc
      961 ggcatcgtcc aggaccggga atacttcgag aggcacgtca agccgcacgt cgacggaagg
     1021 agggtcgtgt tcgtggggag cgcggacccg cgcacgcgcg acgagctcct gggaaaggcc
     1081 tacgccctcc tccatcccat caacttcgac gaacccttcg gcctctccgt ggtggaggcc
     1141 atggcgtgcg ggacgccggt catagcgttc aaccgtggaa gcatgccgga gattgtgcgg
     1201 cacggcgtga caggcttctt ggtgagggac gtcgatgcca tggtggacgc ggtcccgcgg
     1261 gtgcgcgacc tcgacagggc ccaggcgcgg cgttgggtcg aggaacgttt cagcgccgag
     1321 cgcatggtgg acgactatgc caggctgtac gagctcatcg tgagccgagg gaggtgagcc
     1381 gtggggaagg agaggaggtc catcgtcgag cggtatcccg gcaacccgat cttgaccaag
     1441 gacgatgtcc cgtacccggt cgagaccgtg cacaacgcgg ccgtggtgaa gcatgcgggc
     1501 gggtacacga tgctgttcag gtcccacctc aaaaacggcc gctcggtgat aggcatcgcg
     1561 gacagccgcg acgggtacgc gttcaccgtc agggacaggc cgtttgtcac cccggcgacc
     1621 gaggggcctt tcgcgcaggt cgaggagttc ggcgtggagg accccaggat caccttggtc
     1681 gagggcgagt accttgtcac ctacagcgcg tactcgcgct tcggcgtgag ggtggtcctc
     1741 gcgaggaccg ccgacttcga ggcggtcgag cgcatcgccg tgatcacgca ggcggactgc
     1801 cgcaacgtgg ttcttttccc cgaaaggatc ggggggaggt acgtgaggct cgaccggccg
     1861 cactcggaga tcatgccgtg gtccatctgg atgtcctctt ccccggatct cgtgcactgg
     1921 ggggagtcga aaccgttgat ctcgcccatg acctaccact gggacgagat gaaggtcggg
     1981 cccgggtctc cccctgtcaa gaccgagcgg ggctggctcc atgtcttcca cggcgtgttc
     2041 aagaccatgg acggcgccgt ataccggctg gggacggccc tgcacgacct cgccgatccc
     2101 tcgcgcgtca taggcgtcgc cgacgaatgg atcctgcagc ccgaagaccc gtgggaggtg
     2161 acggggtacg tacacaacgt ggtcttctgc tgcgccatgg tccccgaaga cgaccggacc
     2221 gcgaagatct actggggggg cgccgatacg gtggtgtgcg cgggtacggt cgacatggag
     2281 gagttggtga gactgtgcat cgacaggccg aggccgcccc tgtgaaggac cgcctttgca
     2341 cgacgacggg ggttcccaag gcgcacccgt tggggtagaa gagccccatg gagatctcgt
     2401 tcaagcccat aggggtgatg cactcccccc tgaaggacgc cgagaccgcc cccaggttct
     2461 acacggagtc ggacgtcaag ggggtcatag aggtctatcc cgagtacgag gcggggctct
     2521 ccaacctctc ggactacacg cacatcgtcg tggtgttcta tttccacaag tcgagggggt
     2581 acgagctgtt gcagaggcga aggggggtgg gcgagctgcg cggggtgttc agcctgtgct
     2641 cccccaaccg gcccaacccc atcgggatct ccgtgctgag gctcctcctt gtcgagggga
     2701 acatgctcca cgtggacaac gtcgacatcc tggacgacac ccccatcctc gacatcaagc
     2761 cctacaagcc cttcgactac ccccaaggcg gctgaggccg gttctccccg gtgccggatc
     2821 aacaggcggc gtgattgcct tggagggtcg gccgggagga aaagtgcatt gaatttttcc
     2881 ctctgatatg gttggttgtg cactggcaag gagaggggtc gaggtgcgcg cacacgagcc
     2941 ggaggtgtcc tcgagaccat ctcgggaggc ccccggcgcg ctctcgaggc ccctcgaggc
     3001 ctggatacag gctgcaagcg aggtgtgccg ggaggacaaa caaccatgga atacgacatg
     3061 ccgctgttca ggccgccgtc cgaggcgcgc tccctcatct tccaggtgac gctcggctgt
     3121 tcctggaaca ggtgcgtgtt ctgcgcgatg tacaagacga agaagttcag ggtgagacct
     3181 ttcgaagagg ttgagaagga cgtcatcgag atgtcgtggc tctacccggg gacggagaag
     3241 atcttcctgg ccgacggaga ccccctggcc gcgccgaccg ggtacctgat cgacgtcctc
     3301 gacctcatga accagaggtt ccccaacctg aaaaggataa gcgcgtacgc cggtcccacg
     3361 aacctcctcg agaagtccgt cgacgagctc agggaactga ggaagagaaa gctcgacgtg
     3421 ctgtacctgg gcatcgagac cggcaacgac gcactcctga agcggatcag gaagggggcg
     3481 accgccgccg ggatcatcca ggggtgccgg aaggccgtgg aggccgggct tcgcatgtcg
     3541 accttcatca tcctcggtct cggaggcgtg gacgggagct acgtccatgc caaggactcg
     3601 gcgagggtgg ccaacgccat cgacccgcac ttcctcgcca cgctcacgct catgctcggg
     3661 ccgtatgagc gcgtctacga ggaaaagatc atgggaggcg gcttcaagct catcgacaag
     3721 cgccagtcgc tccaggagct caggtggttc gtggaggacc tggaggtctc cgactgcaag
     3781 ttcgggtcgg aacacgcgtc gaactacctt cccataagcg ggtacctgcc ccgcgacaag
     3841 gaagagatcc tgcggctcat cgacaccgcc ctgtcggatg cgtcctcggg gcttctcagg
     3901 ccggagtggg ctcgggggct ctgatggaca tccgggctgt acggggcatg aacgacatcc
     3961 tccccgagga ggccgtcctg tggaggttcg tcgaggaaga ggccaggagg atcttcgcgc
     4021 tctacgggtt cgaggagctc aggccgcccc tcttggagcg cacggaactc ttcttgaggg
     4081 gcatcggcga cgagaccgac gtggtggaga agcagatgta caccttcacg gacaagggcg
     4141 gcgattcggt cacgctcagg cccgaggcca ccgcgtgcgt gctcagggcg gtgatcgagc
     4201 acggcatcct gaacaaggac cccgtcgcca agctctacac cataggcccc atgttccgct
     4261 acgagagacc acagaaaggc cgcttccggc aattccacca gataaacgcg gagcggctcg
     4321 gcgaggacgg tcccctggcc gacgccgaga cgctggccat ggcctacgat atcgtgagga
     4381 gcgtactggg gggcgagggg ctggtcatgg aggtgaactc cctgggatgc aagcagtgca
     4441 ggccggcgtt cacggaggcg ctcaaggcct ttctcgcggc cagggaggac ctcttgtgct
     4501 cggactgcag gcgcaggatg gtatcgaacc cgctacgggt gctggactgc aaggtcccga
     4561 cctgcaaaga agccgtgatg gatgcgccca ccttggatgc gacgctgtgc ggcggatgca
     4621 gggagcacca cgagtgcgtc aaggaggggc tcgatgcgct cggcgtgcgt tacgagcaga
     4681 acgtgcacct ggttcgtggg ctcgattact acacacggac gacattcgag atcgtctcgg
     4741 cgtccctggg gtcgaacagc gcagtggcgg gaggcgggag gtacgacgac ctgttgggcc
     4801 tgctgggggg gccgcctgtg ggagggatcg gcttcgcgat agggatggag cggctcgtca
     4861 tgctcgcgcc cgaagggctc gcgaagaagg gggaagggtg cttcgtggcc gcccagaaca
     4921 cgcacgaggc acggcttgag gcgctcaggc tgatgatgga attgcgcggg aacgggatcc
     4981 cggccgactc cgccctcggc aggagcttca aggcgcagat gcgaagggcc gacaagggcg
     5041 gacaccccgt gtgcgtcatc gtgggcgaag aggaactgag gaccgggacc gtgagcctca
     5101 gggacatgcg gacgggttcc caggagacgg tcccgagggc ggacctcgtc tcgaggatcc
     5161 tctccgttct ggacaaggga aaaggagaaa aggcgtgaag actgtttcgg caggcatggc
     5221 aaggacccac tactgcggcg agatcgggga cgcgccggag gggagccggg tgacgctgta
     5281 cgggtgggtg cagcggagaa gggacttggg cggcctcatt ttcatagacc tcagggaccg
     5341 caccggcata gcccaggtgg tc
//
LOCUS       DAOSCR010000110         5355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9690_length_5355_cov_4.118302, whole genome shotgun sequence.
ACCESSION   DAOSCR010000110 DAOSCR010000000
VERSION     DAOSCR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9690_length_5355_cov_4.118302"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PLT69_04085"
     CDS             <1..1188
                     /locus_tag="PLT69_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13652.1"
                     /translation="QMWAFGVALMGGMTMNFPETPETVTEDFREIGPELLITSSRFWE
                     DLASRIRVKIDDSSAINKAAFTVAERIGRACVERDSRQGGPPAGLRVLHRIARTLVFN
                     PLLDRVGCSRLLAAYTGGHPISPDVIRFFRACGLNLKQCYGMTETCGIFQVQPDDEVK
                     PETVGKTLPKTSVKISPDQEVMVLGESVFTGYYNDFEATDEAFENGWFKTGDAGYLDD
                     DGHLVIIGRKQDIIRDKHGNAFSPDFIETRLKFSLYIKEAVVFGEGRPFITALINVDQ
                     GNTGNWAEERMIPYTTYTDLSQRPEVEHLILDEVRKVNAQLPPPMQVKRFILLYKLLD
                     ADDEELTRTGKVRRRFVYGLYLNLIEAMYQGLAEVAVKGKIRYRDGTVGMIETTSRVI
                     TVF"
     gene            1214..2092
                     /locus_tag="PLT69_04090"
     CDS             1214..2092
                     /locus_tag="PLT69_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ13653.1"
                     /translation="MDFFIHLFINGLSVGLLYGVSALGFVMIFKSSSVLNFAHGQLLA
                     IGAFAFLVLVSWLKLPVALAFLVTLCGSFALGFAVERVFLRPLIGESLIFVIMLTVGL
                     ASMLKGILLLFFGGNLQTYPDFLPGWLAVQAGSVYIPPVYVASSLLGIVFLVLFGLFF
                     KYSPQGIFMRSVADNQTAALSLGVHVKKVFALSWAIAAMVASMSGVMLGIINGINVHE
                     ISSIGLKVFPVVILGGLDSIGGAIIGGMIIGLLETFTSGYISPSLRDVIPYIVLVAIL
                     LVKPYGLFGLVEIERV"
     gene            2101..3171
                     /locus_tag="PLT69_04095"
     CDS             2101..3171
                     /locus_tag="PLT69_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ13654.1"
                     /translation="MRRLRFHPCGNYKEDRLEQLFLFETDFGRACTLAGLFLLFVVLP
                     LASPPYLIYVLNITGIAAIAALGLNILVGFTGQISLGHGAFFGVGAYSAAVLATRLQM
                     PFWVCVPAAGIVTALVGLVFGIPSGRLKGLYLTIATLAGQFIIEYVLVHWESLTQGSM
                     GIVLPQAHLFGLPVSSDRAFFLLIFGVLVAMTLMASNVMRTSYGRAFVAIRDNDRAAE
                     GMGIPVFGYKLLSFAMSSFYAGVAGGLWAYYMRSISTEPFTLGLSVEYLAMVIIGGLG
                     SIPGSLFGAVFITGLNEILRYLTDAMMNMGISSGFGLNLASLREFAFGLAIVVFILFE
                     PKGLAEIWRIVRSSFRLWPFSY"
     gene            3181..3990
                     /locus_tag="PLT69_04100"
     CDS             3181..3990
                     /locus_tag="PLT69_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13655.1"
                     /translation="MGASNESLLVINNISVVYSDVIQVIKGVSLKVPRGRIVSLLGSN
                     GAGKTTTLKAVSGLLKPENGRVTDGEIVFDGEKIHNLSPEEITRLGIIQVLEGRQPFK
                     YLTVEENLRVGTATRWMKPYRQDLDLVYHYFPSLVQRRKTLAGYLSGGETQMLVMGRA
                     LMAHPRLLLLDEPSLGLAPLVVREIFRIIKRINEEQKTTIVLVEQNANMALRIAHYGY
                     VMENGRVVMEGDSGYLRGNPDVKEFYLGVSAGGASRSYREVKSYKRRKRWL"
     gene            4058..5314
                     /locus_tag="PLT69_04105"
     CDS             4058..5314
                     /locus_tag="PLT69_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13656.1"
                     /translation="MGRYLDDLETQSMQMRAERQSRLLADMVRRGYERSHRVRAIFDE
                     SGLSPADIRSVSDLERLPVINRERIVRMEEEEPPYGGLADVSARIERIFTSPGPVYEP
                     HLNSDDPLWARAYKAAGFGPGDIVLNAFSYHMVAAGLTFHGGLVRVGATVVPSGTSPA
                     HQQVQLIKDLKVTGYTGTPSFLMAIIDKANEMGYDFARDFSLRRAAFAAEPLQPSLRG
                     RFERDFGIDTYQMYGATEVGDVAYECREKNGWHLCEEVIVEIVDPETGKPLGPGELGE
                     VVVTRLNPIFFLFRFGTGDLSRLTDAPCPCGRTSLRLEGIAGRVGEAVKVRGMFVAPS
                     QVKRLTDMFPGIVVQLVVSRSGTRDVLTARIDASGAGPASTGLAERFEKAFQEVCTVR
                     LDRLELVEPGTLAADAKPLLDERTWE"
BASE COUNT          946 a         1707 c         1711 g          991 t
ORIGIN      
        1 cagatgtggg ccttcggcgt ggcgctcatg ggcggcatga ccatgaactt cccggagacc
       61 cccgagaccg tgaccgagga cttccgggag atcggcccgg agctcctcat cacctcgtcg
      121 aggttctggg aggacctcgc cagcaggatc cgggtgaaga tagacgactc gtccgccatc
      181 aacaaggcgg cgttcacggt cgccgaacgc ataggaaggg cgtgcgtcga gcgggactcc
      241 cgtcagggcg ggcccccggc cggcctcagg gttctccacc gcatcgcgag gacgctcgtc
      301 ttcaaccccc tgctcgacag ggtggggtgc tcgaggctcc ttgccgccta caccggaggc
      361 caccccataa gccccgacgt gatacgcttc ttcagggcct gcgggctcaa cctcaagcag
      421 tgctacggca tgaccgagac ctgcggcatc ttccaggtgc agccggacga cgaggtgaag
      481 cccgagaccg tgggaaagac cctgccgaag acctccgtga agatctcccc ggaccaggag
      541 gtcatggtcc tcggggaatc tgtcttcacc ggttactaca acgacttcga ggcgaccgac
      601 gaggccttcg agaacggctg gttcaagacc ggggacgcgg ggtacctcga cgacgacggt
      661 cacctcgtga tcatcggccg caagcaggat atcatcaggg acaagcacgg gaacgccttt
      721 tccccggact tcatcgagac ccgcctcaag ttctcgctct acatcaagga ggcggtggtc
      781 ttcggggagg gcaggccttt catcaccgcc ctcatcaacg tcgaccaggg aaacacgggc
      841 aactgggccg aggagcggat gatcccctac accacctaca cggacctttc gcagcgcccg
      901 gaggtggagc acctcatcct cgacgaggtg aggaaggtga acgcccagtt gccgccgccc
      961 atgcaggtga agcggttcat cctgctgtac aagctgctcg acgccgacga cgaggagctt
     1021 accaggacgg ggaaggtgag gaggagattc gtctacggtt tgtacctgaa cctcatagag
     1081 gccatgtacc aggggctcgc ggaggtggcg gtgaagggga agatccgcta ccgggacggc
     1141 accgtgggca tgatcgagac gacttcccgg gtcattacgg tcttctgagg cttgaaaggc
     1201 ggggacaagg aacatggact tcttcattca tctgttcatc aacggtctca gcgtgggatt
     1261 gctctacggg gtctcggccc tcgggttcgt catgatcttc aagtcatcct cggtgctcaa
     1321 cttcgcccac gggcagctgc tcgcgatcgg cgccttcgcc ttcctcgtcc tcgtgtcgtg
     1381 gttgaagctc cccgtggccc tcgccttcct cgtcacgctg tgcgggagtt tcgccctcgg
     1441 cttcgccgtg gagcgcgtct tcttgaggcc gctcataggc gagtccctca tcttcgtcat
     1501 catgctgacc gtgggtctcg cctccatgct caaggggatc ctgctcctct tcttcggggg
     1561 caacctccag acctacccgg acttcctgcc cgggtggctc gccgtgcagg cgggcagcgt
     1621 gtacatcccc cccgtgtacg tggcgtcctc cctgctgggg atcgtgttcc tcgtgctctt
     1681 cgggctcttt ttcaagtact cgccccaggg catcttcatg aggtcggtcg ccgacaacca
     1741 gacggcggcc ctgtccctcg gtgtccacgt gaagaaggtg ttcgccctct cgtgggcgat
     1801 cgcagcgatg gtcgcttcca tgagcggggt catgctcggc atcatcaacg gcatcaacgt
     1861 gcacgagata tcctccatcg ggctcaaggt cttccccgtc gtgatcctgg gcggcctcga
     1921 cagcatcggg ggcgccatca tcggcggcat gatcatcggg ctcttggaaa ccttcacgag
     1981 cggctacatc tccccatccc tgagggacgt gatcccgtac atcgtgctgg tcgcgatcct
     2041 cctcgtcaaa ccctacggcc tcttcggcct ggtggagata gaacgggtct aggagacaag
     2101 atgcgaaggc tgcgctttca cccgtgcgga aattacaagg aagaccggct ggaacagctc
     2161 tttctgttcg agacggactt cgggagggcc tgcaccctcg cgggcctctt cctgctcttc
     2221 gtcgtcctgc ccctcgcaag cccgccatac ctcatctacg tgctcaacat caccggcata
     2281 gcggccatag cagcgctggg actgaacatc ctcgtcgggt tcaccgggca gatatccctc
     2341 ggccacggcg ctttcttcgg cgtgggcgcg tactcggccg ccgtcctcgc cacccgtttg
     2401 cagatgccct tctgggtttg tgtacccgca gcgggtatcg tcacggcgct cgtgggcttg
     2461 gtgttcggca tcccctcggg caggctcaag gggctctacc tgacgatcgc caccctcgca
     2521 ggccagttca tcatcgagta cgtgctggtg cactgggaat ccctgaccca ggggagcatg
     2581 ggcatcgtgc tcccgcaggc gcatctcttc ggcctcccgg tctcctcgga cagggccttc
     2641 ttcctcctga tcttcggggt cctcgtggcc atgacgctca tggcgtcgaa cgtcatgcgc
     2701 acgagctacg gaagggcctt cgtggccatc agggacaacg accgggcagc cgagggcatg
     2761 ggcatccccg tcttcggcta caagctgttg tcgttcgcca tgagctcgtt ctacgcgggg
     2821 gtggcaggcg gcctctgggc gtactacatg aggagcatct ccactgagcc cttcacgctg
     2881 ggcctctccg tcgaatacct cgccatggtg atcataggcg ggctcggcag catacccggt
     2941 tccctgttcg gcgccgtgtt cataactggt ctcaacgaga tcctcaggta cctgaccgac
     3001 gccatgatga acatggggat atcgagcgga ttcgggctca acctcgccag cctccgggag
     3061 ttcgcgttcg gcctggccat cgtcgtcttc atcctgttcg agcccaaggg gctcgccgag
     3121 atctggagga tcgtccgctc gtccttcagg ctgtggcctt tttcctattg aggtgcgacc
     3181 atgggtgcga gcaacgagag cctcctcgtg atcaacaaca tatcggtcgt gtactcggac
     3241 gtgatccagg tgatcaaggg ggtgtccctg aaggtgccac ggggcaggat cgtgtccctg
     3301 ctggggagca acggcgcggg aaagactacc acgctgaagg ccgtctccgg gctgctgaag
     3361 ccggaaaacg gccgggtcac cgacggggag atcgtcttcg acggcgagaa gatccacaac
     3421 ctctcgccag aggagatcac ccgtctcggc atcatccagg tcctggaagg caggcagccc
     3481 ttcaagtacc tgaccgtgga agagaacctg agggtcggga ccgcaaccag gtggatgaag
     3541 ccctaccggc aagacctcga tctcgtctac cactacttcc cctccctcgt tcagaggaga
     3601 aagacgctcg ccgggtacct gagcggcggg gagacccaga tgctggtcat gggccgcgcg
     3661 ctcatggccc acccgaggct cctcctcctg gacgagccct ccctggggct cgcccccctg
     3721 gtggtgcggg agatattccg gatcatcaag cgaatcaacg aggaacagaa gacgaccatc
     3781 gtgctcgtcg aacagaacgc gaacatggcc ctgaggatag cccactacgg gtacgtcatg
     3841 gaaaacggcc gggtggtcat ggagggggat tccggctacc tgagggggaa tcccgacgtg
     3901 aaggagttct acctgggcgt gagcgcaggg ggcgcctccc gctcgtatcg cgaggtgaag
     3961 tcgtacaaga ggagaaagcg ctggttgtaa cccttcgtga aagcccaggg gcgctgcatg
     4021 cacgacatgc ctggaccact ggggaaagga gctgtgcatg ggaaggtatc tcgacgatct
     4081 cgaaacgcag tcgatgcaga tgcgggcgga gaggcagtca cgcctcttgg ccgacatggt
     4141 ccgtcgcggc tacgagcggt cacacagggt gagggcgatc ttcgacgagt cgggtctttc
     4201 gcctgccgat attcgttccg tctccgacct ggaaaggctc cccgtgatca accgcgaacg
     4261 gatcgtccgg atggaggagg aagaaccccc gtacggcggg ctcgcggacg tatcggcgcg
     4321 catcgagcgc atcttcacct cgccgggacc ggtctacgag ccccacctga actccgacga
     4381 ccccctgtgg gctcgtgcgt acaaggcggc ggggttcggg cccggagaca tcgtgctgaa
     4441 cgccttctcg taccacatgg tggccgccgg cctcacgttc cacggcggcc tggtccgcgt
     4501 gggcgcaacc gtggtgccgt ccggcacttc gcccgcccac cagcaggtcc agctcatcaa
     4561 ggacctcaag gtgacgggtt acacgggcac cccgagcttc ctcatggcca tcatcgacaa
     4621 ggcgaacgag atggggtacg acttcgcccg cgacttctcc ctgaggaggg cggcctttgc
     4681 ggccgagccc ctgcagccct cgctccgcgg gagattcgag cgcgacttcg gcatagacac
     4741 ctaccagatg tacggcgcca cggaggtggg cgacgtggcg tacgagtgcc gggagaagaa
     4801 cggctggcac ctctgcgagg aggtcatcgt ggagatcgtg gaccccgaga ccggcaagcc
     4861 cctgggcccg ggcgagctcg gcgaggtcgt ggtcaccagg ctcaacccca tcttcttcct
     4921 gttccgcttc ggcaccggag acctctcgag gctgacggat gcgccgtgcc cgtgcggcag
     4981 gacgtccttg aggctcgaag gtatcgccgg gcgggtcgga gaggccgtca aggtcagggg
     5041 catgttcgtg gcgccgagcc aggtcaagcg ccttacggac atgtttcccg ggatcgtggt
     5101 ccaactcgtc gtgagcaggt cgggcacgag ggacgtcctc acggcgagga tcgacgcgtc
     5161 gggagcgggc ccggcatcaa ccgggctggc cgagcgcttc gaaaaggcct tccaggaggt
     5221 ctgcacggtg cggctcgaca ggctggaact cgtcgagccg gggaccctgg cggccgacgc
     5281 gaagccgctt ctggacgaac gcacctggga gtgagacgcc cggggttgtg catccgttga
     5341 ggcatggcaa agccc
//
LOCUS       DAOSCR010000111         5332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9736_length_5332_cov_3.432822, whole genome shotgun sequence.
ACCESSION   DAOSCR010000111 DAOSCR010000000
VERSION     DAOSCR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9736_length_5332_cov_3.432822"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="PLT69_04110"
     CDS             <1..1725
                     /locus_tag="PLT69_04110"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOJ13657.1"
                     /translation="AAQFDCTGAGIQTVRATVNAGTRAVASGGPWPCTTGEGVIGGVE
                     AGSGYTVTVELLDVDGRVQYRGTSRSFDVVPGRVNDAGTITPLPVNSPPVFEQIAPIS
                     AAYGTLVTFTVHASDPDGDLVALSALSLPSGAEFDTQTGVFTWLPSQYQSGTHTVRIQ
                     AVDDAVEPLSAELSVTIVVGGAPVFNQVGTQSVAEGRTLSFTVDAVDPEGTRVTYAAV
                     SIPQGASFDSDKAVFTWTPGYTQSGNYTARFSATDEEGLSSTLDVAISVTNTNRPPVI
                     SVPSSPVHFYATGVSNRLTISATDPDGDSLTFDAAEMPTDWWGSYIDGPVFTPSTRTF
                     VWGEPGTANAGEYKVLFRVTDSGNPKLGDTAYVTIQVYDTASQLQDNTYPVLSPIGSQ
                     RGAIGQQLQFTVSATDADGDSLTYTAASIPGKNAPSGYTFADRVFSWTPSANGNYWLR
                     FTVSDPDDHAGYTTSDSEDVVITVGNVNRPPVLDPIGRRAVYNNLTFSFVVTATDPDG
                     DRVTYTATNLPRGASFDASTQTFTWDVVDFPVGSSYTVRFTATDSGSPQESDYEDVVF
                     TVVYPPLI"
     gene            1756..2901
                     /locus_tag="PLT69_04115"
     CDS             1756..2901
                     /locus_tag="PLT69_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13658.1"
                     /translation="MRETTGTRMFVAAIVCTVAILGLPDAGLGMERSFNTGLEVSWWK
                     TDEGDSGNQVVVPLAAAARGEAFRFSLLSAIVRTSVKPDDGSSATLAGAADTKADLSY
                     QVQDRLPVDLLAGIGFNLPTGRTDLDEEGLAVAALPSELLAINGIGGGFDVNPYLVAA
                     RQFDGWSAGFGLGYLVRGKYDYSDALEDLDPGDVLTLTARVSHECTDAVQGRLFAEYA
                     HYGKDELDGQEYYRDGDLFLVGLGASASLPAWRLEATVSGVFRKKAAFYTTSSTPVEK
                     EKNYGDELHVDLRHWLHLRSTTTVVSHAGVLVMGANGFDEESGLRHEGMVKVSAGAGM
                     EKKITDACTCAFDLSVFSLKDKANWFHPDEELTYSGFTVKAHARWAF"
     gene            2970..3533
                     /locus_tag="PLT69_04120"
     CDS             2970..3533
                     /locus_tag="PLT69_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOJ13659.1"
                     /translation="MDARKLALVALFAALTAVGALIKIPLWPVPMTMQTFFVLMAGIV
                     LGPRHGTLSQAVYILVGLMGLPVFSGGGGPSYLLSPTMGYLIGFVLAAWAAGLAAGGG
                     RISFGRALGASVTGTAAIYLVGVPYLALYMHLVLHKPEAVGVALKTGLAVFVPGDILK
                     CLLLTAVAPRIAALLAQREGRHARHDS"
     gene            complement(4153..>5332)
                     /locus_tag="PLT69_04125"
     CDS             complement(4153..>5332)
                     /locus_tag="PLT69_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOJ13660.1"
                     /translation="SGTETAYMRVNRFRMKSLAEKGNRRARAVEEILAEPERLISTIL
                     LGNNFVNVLASAIATALFISFFGEEGILYATIAMTVLLLLFGEITPKTIAAYRSDMVA
                     LLFGTPVKALTVVLKPFVHVFTVAARWVVRLLGMKGPGHHDFSEEDLGSVIAMGHQEG
                     FLSEPKARMLSAIMELDEVPVRKVMLPIGEMVCVPEESTFDGILQVIATRSFSRYPVY
                     RETADNIVGYLHIRDLWTHLDRRDVFRVKDCMREAHFVPETTSIFTQLVVFQNMHLHM
                     AFVVDEYGTVKGAITLEDIIEEITGDIADEHDIVRPAIVPVSPTSFIVTGSISLLDLG
                     RHIGRQFPDEIDTLSGLIYHTLDRIPEEGETLVYEDMRLKIERMRGNRIVRVRITMGG
                     "
BASE COUNT          906 a         1820 c         1733 g          873 t
ORIGIN      
        1 gcggcccagt tcgactgcac gggtgccggg atccagacgg tcagggccac ggtcaacgca
       61 ggcacgaggg cggtcgcctc aggcggcccc tggccgtgca cgaccgggga gggcgtcatc
      121 gggggcgtcg aggccggaag cggctacacc gtcaccgtcg agctcttgga cgtggacggc
      181 agggttcaat accggggtac gagcaggtct ttcgacgtgg tcccaggcag ggtcaacgac
      241 gcggggacca tcacccccct gcccgtcaac tcgccgcccg tcttcgagca gatcgccccc
      301 atcagcgccg catacggcac gctcgtgacc ttcaccgtgc acgcgagcga cccggacggc
      361 gacctggtcg ccttgagcgc cctgtcgctc ccttcgggcg cagagttcga cacgcagacc
      421 ggggtcttca cctggttgcc ctcgcagtac cagtcgggga cgcacacggt gcgcatccag
      481 gcggtggacg acgccgtgga gcccctgagc gcggagcttt ccgtcaccat cgtggtgggg
      541 ggcgcccctg tgttcaacca ggtggggacg cagagcgtcg ccgagggcag gaccctttcc
      601 ttcaccgtcg acgcagtcga ccccgaaggg acgagggtga cctacgcggc ggtgtccatc
      661 ccgcaaggtg cgagcttcga ctcggacaag gccgtcttca cctggacccc gggctacacc
      721 cagagcggga actacaccgc ccgcttctcg gccaccgacg aagaagggct gagctcgacg
      781 ctcgacgtgg cgatcagcgt gacgaacacg aacaggccgc ccgtcatcag cgtcccgtcg
      841 agcccggtgc acttctacgc caccggcgtc tccaacaggc tcaccatctc cgcgacggac
      901 ccggacgggg acagcctcac cttcgacgcc gccgagatgc cgaccgactg gtggggctca
      961 tacatcgacg gccccgtctt caccccctcc acgcggacct tcgtctgggg cgagcccggt
     1021 accgcgaacg cgggcgagta caaggtgctc ttcagggtga cggacagcgg aaacccgaaa
     1081 ctcggcgaca ccgcctacgt cacgatccag gtatacgaca ccgccagcca gcttcaggac
     1141 aacacatacc cggtgctgtc cccgatcggc tcacaacgag gcgccatcgg ccagcagctc
     1201 cagttcaccg tgtccgccac ggacgcagac ggcgactcac tcacgtacac ggccgcctcc
     1261 atccccggca agaacgcgcc ttccgggtac accttcgcgg acagggtgtt ctcctggacg
     1321 ccgtctgcga acgggaacta ctggctcagg ttcacggtga gcgatcccga cgaccacgcg
     1381 ggctacacca cgtccgactc cgaggacgtg gtgatcacgg tcgggaacgt caaccgcccg
     1441 ccggttctcg accccatcgg caggagggcc gtctacaaca acttgacgtt ctccttcgtg
     1501 gtcacggcga ctgacccgga cggcgacagg gtcacgtaca ccgccacgaa cctgcccagg
     1561 ggggcgagct tcgacgcatc cacgcagacc ttcacctggg acgtcgtcga cttccccgtg
     1621 gggagctcgt acaccgtccg cttcacggcc accgatagcg ggagcccgca ggagagcgac
     1681 tacgaggacg tggtgttcac ggtggtgtat ccgcccctga tctgaccttc ccggatggag
     1741 agaaagggcc tgtttatgag ggagaccaca ggaacgagga tgttcgttgc ggcgatcgtc
     1801 tgcaccgtcg cgatcttggg cctacccgac gccggcctcg gcatggagag atccttcaat
     1861 acagggctcg aggtctcgtg gtggaagacc gacgagggcg actcgggcaa ccaggtggtc
     1921 gtccccctcg ctgctgcggc aaggggcgag gcgttcaggt tctccctcct ctcggcgatc
     1981 gtgaggacgt ccgtgaagcc agacgacggg tcgtccgcca cgctcgccgg ggccgcggac
     2041 accaaggcgg acctctccta ccaggtgcag gaccgcctcc ccgtcgatct cctcgcgggc
     2101 atcggtttca acctgccgac cgggcggacg gatctcgacg aagaaggcct ggctgtcgcg
     2161 gccctgccct cggaactgct cgccataaac ggtatcggcg gggggttcga cgtgaacccc
     2221 tacctcgtcg cggccaggca gttcgacggg tggtcggccg gcttcgggct cggctacctc
     2281 gtcaggggaa aatacgacta cagcgacgcc ctcgaggacc tcgaccccgg cgacgtgctc
     2341 acgctcaccg cgcgcgtctc ccacgaatgc acggacgccg tccagggaag gctcttcgcg
     2401 gaatacgcac attacggaaa ggacgagctc gacggacagg agtattaccg cgacggtgac
     2461 ctcttcctcg tcggcctggg cgcatcggcc tcgctgcccg cgtggaggct cgaggccacg
     2521 gtctcgggcg tgttcaggaa gaaggccgcc ttctacacca catcctcgac gccggtcgaa
     2581 aaagagaaga actacggcga cgagctgcac gtggacctca ggcattggct ccacctgaga
     2641 tccaccacca cggtcgtatc ccacgcgggc gtcctcgtga tgggcgcaaa cggcttcgac
     2701 gaggaatccg ggctcaggca cgagggcatg gtcaaggtct ccgcaggcgc cggcatggag
     2761 aagaagatca cggacgcatg cacctgcgcc ttcgacctga gcgtcttctc cctgaaggac
     2821 aaggcgaact ggttccaccc ggacgaggag ctcacgtact cggggttcac cgtcaaggcc
     2881 cacgcccgct gggccttctg agagacctga ccgtaggagg cctcgggccg tttacttttc
     2941 cgccggcctg ctttacatag ggaagccgta tggatgcacg caagctcgcc ctggtcgctc
     3001 tcttcgcggc gctgactgcc gtcggcgccc tcatcaagat ccccctgtgg cccgtcccga
     3061 tgaccatgca gacgttcttc gtcctcatgg ccggcatcgt gctcggcccc aggcacggaa
     3121 ccttgagcca ggcggtctac atcctggtcg gcctcatggg gctgccggtc ttctcgggag
     3181 gtggcggccc ttcctacctc ctgagcccca ccatgggcta cctcataggg ttcgtcctcg
     3241 ccgcatgggc cgcaggcctg gcagccgggg gcggcaggat ctccttcggc agggccctgg
     3301 gtgcctctgt cacggggacg gcggcgatct acctagtggg ggtcccgtac ctcgccctct
     3361 acatgcatct cgtgctccac aagccggagg ccgtcggtgt ggcgctcaag accgggcttg
     3421 cggtgttcgt gcccggggac atcctcaagt gcctgctgct gacagccgtg gcgccgagga
     3481 tcgccgccct gctggcgcag cgggaagggc gccacgcgcg gcacgattca tgatgtgggc
     3541 ggacaggacg gccgcgatcg cggccgtcct caagcgcggc ggcacaactc catctacgag
     3601 agaagacgtg ttcttcaggg cccggggacg agacgggtcc gtccaccgac accgccagca
     3661 aagccttccc cgtgccccgc gcaggcaggc cctgcactct caagcgccgc gagaccatgg
     3721 tgaccgctcg cacggccttc cccggtcgcc tgttcatgcg gatcctgaag attatggcac
     3781 tacgggtcgg tcggtgaagg ctgcatgata cccagcgggt ggcgccgcct ctcaggcaca
     3841 cccgtgagcc cctcgtgcac ggggccacga gcggtggaga gctgggtgca tggcctcgcc
     3901 cggtgtgagc ctctcgtacc cggggccaag aggccagtgg ggcctgtccc gcggcgatca
     3961 cgcgcgccag accctcgtgc ccaagggcaa gaggcactgt gccccttggc caccgcgggc
     4021 gtgaaggcga ccctgcacgg cccggcccgt acgtagagac ccttcgatcg gcttcctgac
     4081 ccgcgggatg aggccgcggc gaacagggac ccgcgagggg ggagtccctc gcccccgccg
     4141 tccccgcctg tctcaaccgc ccatggtgat cctcacgcgg acgatcctgt tgccgcgcat
     4201 ccgctcgatc ttgaggcgca tgtcctcgta gacgagggtt tccccctcct cggggatccg
     4261 gtcgagggtg tggtagatga gaccagacag ggtgtcgatc tcgtccggga actggcggcc
     4321 gatgtggcgg ccgagatcca gaaggctgat gctcccggtc acgatgaagc tcgtggggct
     4381 cacggggacg atcgctggcc tcacgatatc gtgctcgtcc gcgatgtccc ccgtgatctc
     4441 ctcgatgatg tcctcgagcg tgatggcgcc cttgaccgtc ccgtactcgt ccaccacgaa
     4501 cgccatgtgg aggtgcatgt tctggaacac cacgagctgg gtgaagatgg aggtggtctc
     4561 cgggacgaag tgggcctccc tcatgcagtc cttcaccctg aagacgtccc tcctgtccag
     4621 gtgggtccag aggtcgcgga tgtgcaggta ccccacgatg ttgtcggcgg tctcccggta
     4681 cacggggtac ctgctgaaac tcctcgtggc gatgacctgg aggatgccgt cgaaggtgct
     4741 ctcctccggg acgcacacca tctcccctat ggggagcatg accttgcgca ccgggacctc
     4801 gtcgagctcc atgatggcgg aaagcatcct cgccttgggc tcgctcagga aaccctcctg
     4861 gtgtcccatg gcaatgaccg accccaggtc ttcctcggaa aagtcgtggt gtcccggtcc
     4921 cttcatgccc aggagcctca ccacccagcg cgcggcaacg gtgaacacgt ggacgaaggg
     4981 cttgagaacg accgtcaggg ccttcaccgg cgtgccgaag agcagggcga ccatgtcgga
     5041 ccggtaggcg gcgatcgtct tgggcgtgat ttccccgaag aggaggagga gaacggtcat
     5101 ggcgatggtc gcgtacagga tgccctcctc gccgaagaag gagatgaaga gggcggtcgc
     5161 gatcgcggag gcgaggacgt tgacgaagtt gttgccgagg aggatggtgc tgatgagccg
     5221 ctccggctcc gcgaggatct cctccaccgc ccgtgcccgc ctgttcccct tctcggcgag
     5281 cgacttcatg cggaacctgt tgacgcgcat gtaggccgtc tccgtccccg ag
//
LOCUS       DAOSCR010000112         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9740_length_5331_cov_3.149356, whole genome shotgun sequence.
ACCESSION   DAOSCR010000112 DAOSCR010000000
VERSION     DAOSCR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9740_length_5331_cov_3.149356"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLT69_04130"
     CDS             complement(<1..892)
                     /locus_tag="PLT69_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOJ13661.1"
                     /translation="MEYKDYYKVLGVDRGATQEEIKKRYRKLARDCHPDMCKNDPQAE
                     KRFKEINEAYEVLGDPEKRRRYDALGSSWQDGQSFRPPPNFEDIFQGEFFRTGSKPGS
                     RGFTFEFGTGSAGDAGGFSDFFESLFGDLGVGRRRDRKSQSVRGRDLESEITVTLADV
                     HKGATKEIVISGPNGEKRLNVRIPQGARDGTKIRLARQGSSGPGGPGDLYLTIRVAPD
                     DRFRLEGDDIVVDLPLPPWDAALGTTAELETLDGRIKLKVPSGVSSGQRLRVRGRGLA
                     GRGDLYAQVRVVVPQTLSPEE"
     gene            complement(977..1942)
                     /locus_tag="PLT69_04135"
     CDS             complement(977..1942)
                     /locus_tag="PLT69_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ13662.1"
                     /translation="MKALPRALPSVIAQFVEKDFEQNLRKIIASSSTYTDLIGGYILE
                     SGGKRIRPRIVSLVGEALGLSHDYYMPFAYTVELMHTASLLHDDVVDGTEIRRSRPTA
                     NQVFGDKPALLAGDFISATAIETMCAIGNMRLALAMVQTIKKMAEGELKELEHAGSFH
                     DDLDVYLEIIYLKTASLFELCSLGPGLIAEVDEPLLARLSSYGRGVGMAFQIVDDIIN
                     LCPDASDNKDAYNDIVEGKSTLPLVLLFRERPEVLKTVGSLSGPEEHREYLVAELEPS
                     IFRQCKDMAADYLERAVNEIRMAGLFSDALADIPKQIMAQIDGRF"
     gene            2105..2995
                     /locus_tag="PLT69_04140"
     CDS             2105..2995
                     /locus_tag="PLT69_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13663.1"
                     /translation="MRRIVFVLAVVLALCAAGCDSNVLGPVAKDDTREADIEEARMAI
                     DDGDYDEAIAILLPHLSATDPDARVAEPLASAYMGRAGLDVTYLIENIDDDGLDSDFD
                     VVASALAMRIVSMDGARFLSTGEEIDDMLDDLEQAVAVMDSLWSSTGDYDHAVQRGLA
                     SALHFVALAGSFVESTVPPSSAGLQGLAPVNGQAYRLYFADAAEREAYLDGLAAALAR
                     RTEILEGMRRDLVNVNDAVNALIDRIGADEDMTEDLGGFLRGILGLSGSGKLTEQDVR
                     ASFDAERLPAYIRGEFLGIL"
     gene            3342..4406
                     /gene="traF"
                     /locus_tag="PLT69_04145"
     CDS             3342..4406
                     /gene="traF"
                     /locus_tag="PLT69_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HOJ13664.1"
                     /translation="MKTSLFVLACMVMMAGAVGAAEYPYVYRGMRPLGMGGAFTAVSD
                     DANALFYNPAGLASIEKVSVSFFPLEFEISENTLDLYGDAMDVDSDSEQEVAEFLRSH
                     MGDRARLAVNLFPHYSMPRFAFGVLGTVRADTEIHDRQYPKAVIHGVNDLGIGAGYAH
                     PLLDETLLLGASAKFIRRQSIMEEYSAVDIASEDFDETIRDDLKDGSGILVDLGMIYK
                     LDNVLLPGLRAGVSASNLVGSKLGDAEDMKSHVDLGLAYVMDLGIGKMTLGADYVDLF
                     SQYSGDDDLAKRIRVGAEWKFPAIASVRAGFHQGYFTCGATIDAWVVRIDALTYAEEL
                     GAYAGQRSDRRYTVSLDFGL"
     gene            complement(4515..>5331)
                     /gene="rfaE2"
                     /locus_tag="PLT69_04150"
     CDS             complement(4515..>5331)
                     /gene="rfaE2"
                     /locus_tag="PLT69_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOJ13665.1"
                     /translation="RMVDIRPEDKLLYHTSAVIASNYLVALLDEASAVAGRIGLDRAH
                     LMGLVRGTLANIERLGGAALTGPVSRGDWDTVRMHVDALERCFPDILPSYLALGWYTS
                     RMARRGFPPEIGQRPCPIGIEDLAGRIDAMRDRGMKIVFTNGCFDILHKGHVSYLEGA
                     RRLGDCLVVGLNSDASVRRLKGPSMPVNPVDARCRVLGALACVDYVVVFEEDTPYELI
                     SRIRPDVLVKGGDWSGRDIVGSDVVQSYGGRVVTLAFEEGYSTSWIIERIRKD"
BASE COUNT          932 a         1740 c         1711 g          948 t
ORIGIN      
        1 tctcttcggg ggagagcgtc tgtgggacaa cgacgcgcac ctgggcatag agatcccccc
       61 tgcctgcaag cccacggccc ctcaccctga gcctctggcc cgaggacacg ccggagggca
      121 ccttgagctt gatcctgccg tcgagggtct cgagctccgc cgtggtaccg agtgcggcat
      181 cccacggagg cagtgggaga tccaccacga tgtcgtcccc ctcgaggcgg aacctgtcgt
      241 ccggtgcgac gcgtatggtg aggtagaggt cccccggccc gccggggccg ctcgagcctt
      301 ggcgggcgag ccgtatcttc gttccgtcgc gggcgccctg ggggatcctc acgttgagcc
      361 ttttctcccc gttcgggcca gagatgacga tctccttggt tgcgcccttg tggacgtccg
      421 cgagcgtgac cgtgatctca gactcgaggt cccttccccg aacggactgg gacttcctgt
      481 cacgtcgcct gcccacgccc aggtccccga agagtgactc gaagaagtcc gagaacccgc
      541 ccgcatcacc cgcggagccg gtcccgaact cgaaggtgaa cccccgtgag ccgggcttcg
      601 agcccgtcct gaagaactcc ccttgaaaga tgtcctcgaa gttcggcggg ggcctgaagc
      661 tctgcccgtc ctgccaggaa ctccccaggg cgtcgtaccg cctgcgtttc tcggggtcac
      721 cgaggacctc gtacgcctcg ttgatctcct tgaagcgctt ctccgcctgg ggatcgttct
      781 tgcacatgtc cgggtggcaa tcccgcgcga gcttccggta tcgtttcttg atctcttcct
      841 gcgtcgcgcc ccggtccaca ccgaggacct tgtaataatc cttgtactcc acagaccacc
      901 tctcactcgc gttcccaagg taaacgtagt gataccaagc ctgtaaatca agggacgagg
      961 atcaagggcc gtgttctcag aacctgccgt cgatctgtgc catgatctgc ttcgggatgt
     1021 ccgccaaggc gtcagagaag agacctgcca tgcggatctc gttcacggcg cgctcgaggt
     1081 agtccgctgc catgtccttg cactgccgga agatggacgg ctccagctcg gccacgaggt
     1141 actcacgatg ctcctcgggc ccggagaggc ttcccaccgt tttcagcacc tcaggtctct
     1201 ccctgaagag gagcacgagc ggcagggtgg acttgccctc gacgatatcg ttgtaggcgt
     1261 ccttgttgtc cgaggcgtcc gggcagaggt tgatgatgtc gtccacgatc tggaaggcca
     1321 tgcccacccc cctgccgtac gaggacaggc gggcgagcaa cggctcgtcc acctccgcta
     1381 tgaggccggg ccccaggctg cagagttcga agaggctcgc ggtcttgagg tagatgatct
     1441 ccaggtagac atcgaggtcg tcgtggaagg acccggcgtg ctcgagttcc ttgagctcgc
     1501 cctcggccat cttcttgatc gtctgtacca tcgcaagggc cagcctcatg ttgccgatgg
     1561 cacacatggt ctctatggcg gtggccgaga tgaagtcccc cgcgagcagg gcaggcttgt
     1621 cgccgaagac ctggttcgcc gtcggcctgg acctgcggat ctcggtcccg tccacgacgt
     1681 cgtcgtggag caggctcgcc gtgtgcatga gttccaccgt gtaggcgaac ggcatgtagt
     1741 aatcgtggga gaggcccagg gcctcgccga cgagcgagac gatcctcggc ctgatcctct
     1801 tgccgccgga ctcgaggatg tagcccccta tcaggtccgt gtacgtggaa gagctcgcga
     1861 tgatcttcct gaggttctgc tcgaaatcct tctccacgaa ctgagctata acagacggta
     1921 gagcccgtgg caacgccttc atcgcatgga gccatcccct gggtctcctg tccgcacctc
     1981 tttgtcagcc gtccagaaac atggtataga tggcacggtc gaccttgaaa gacaaacacc
     2041 atgaaggacc gtcggccacg ggacccatcc cggaggccat gtttgacgat cggcaggagg
     2101 tctcatgagg agaatcgttt tcgtgctcgc cgtcgtgctc gccctctgcg cagcggggtg
     2161 cgactccaac gtgctcggcc ccgtggccaa ggacgacacg agggaggctg acatcgaaga
     2221 ggcgcgcatg gccatagacg acggcgacta tgacgaggcc atcgctatcc tgctccccca
     2281 cctctcggcc accgaccccg acgcacgcgt cgccgagccg ctcgcatccg catacatggg
     2341 cagggcgggg ctcgacgtca cctacctcat agagaacata gacgacgacg gcctggactc
     2401 agacttcgac gtcgtggctt ccgcgctcgc gatgcgcatc gtctccatgg acggtgcacg
     2461 gttcctgtcg acgggggagg aaatcgacga catgctggac gatctcgagc aggcggtcgc
     2521 cgtcatggac tccctgtggt cttccacggg tgactatgac cacgccgtcc agcgcggcct
     2581 cgcatcggcg ctccacttcg tggcactggc cgggtccttc gtggaatcaa ccgtgccccc
     2641 gtcctcggcc ggtctccagg gactcgcgcc tgtcaacggc caggcctacc gcctgtactt
     2701 cgccgatgcc gcagagcgtg aggcatacct cgacgggctg gcagcggccc tcgcgaggcg
     2761 gaccgagatc ctggaaggaa tgaggcgcga tctcgtcaac gtgaacgacg cggtgaacgc
     2821 gctcatcgac cggataggcg cagacgagga catgacggaa gacctaggcg ggttcctgag
     2881 gggcatcctc ggcttgagcg gttcgggcaa gctcaccgag caggacgtga gggcgtcgtt
     2941 cgatgccgag aggcttccgg catacatcag gggcgagttc ttgggcatct tgtagattcg
     3001 catggacata cgaccatgca ggataccctc gtgcgcatgg gtacgtccct ggagatcacc
     3061 cgtggaggcc ccgagatcgt agcatggccc gtgccgttgc cgggtgcgaa cctcgacccc
     3121 ctcgtggggt ggatgaacgg acaggggaga aaaaaccacg gcagcccgtc gggacacagg
     3181 ctcgggcggc actttccggg tggtaccccg ggtgctggcg cgggaaaaaa ccacgaaagc
     3241 ccgtcgggac acgggccttg caggggtgct gcagacggct ctctcgatct ttgggggggg
     3301 aggctgcagg cggtgatcga tctttcttcg agggagcgtg tatgaagacc agtctctttg
     3361 tgcttgcgtg catggtcatg atggcagggg ccgtgggggc cgccgagtac ccgtacgtgt
     3421 accggggcat gcgccccctg ggcatgggcg gtgcgttcac cgcggtctcc gacgacgcga
     3481 acgcgctgtt ctacaacccc gcggggctgg catccatcga gaaggtctcc gtgtccttct
     3541 tccccctcga gttcgagatc tccgagaaca ccctcgacct ctacggagac gccatggacg
     3601 tggactcgga cagcgagcag gaagtggccg agttcctccg gtcacacatg ggggaccggg
     3661 cccgcctcgc cgtcaacctc ttcccccatt actcgatgcc ccgcttcgcc ttcggcgtgc
     3721 tcggcaccgt gcgcgcggac acggagatcc atgacaggca ataccccaag gccgtcatcc
     3781 acggcgtgaa cgacctgggt atcggcgccg gctacgccca ccccctgctg gacgagacgc
     3841 tcctcctggg cgcgagcgcc aagttcataa ggcggcagag catcatggag gagtattcgg
     3901 ccgtggacat cgcgtcggag gatttcgacg agaccatacg ggacgacctg aaggacggca
     3961 gcgggatcct cgtggacctg gggatgatct acaagctcga taacgtcctg ctcccggggc
     4021 tgagggcggg tgtgtcggcg agcaacctcg tcgggtccaa gctgggggat gccgaggaca
     4081 tgaagagcca cgtggacctc ggcctggcct acgtgatgga cctcggcatc gggaagatga
     4141 ccctcggcgc ggactacgtg gacctgttct cccagtactc gggagacgac gatctcgcaa
     4201 agaggatcag ggtgggcgcc gagtggaagt tcccggccat cgccagtgtg cgggccgggt
     4261 tccaccaggg ctacttcacc tgcggggcaa cgatcgatgc atgggtcgtg cgcatcgacg
     4321 ccctcaccta tgcagaggaa ctcggcgcgt acgcgggaca gcgttcggac agacgctaca
     4381 ccgtcagcct cgacttcggg ctctgatcgc cttgggggcg cacgagggcc ggcgcccgtc
     4441 tcgccccccg gatcccctcg cgccgccttg ggtgaacgat gccccctcga ggcggacgca
     4501 cccggaggcg aggctcaatc cttcctgatc cgctcgatga tccaagaggt cgagtacccc
     4561 tcctcgaagg ccagggtgac gacccggccc ccgtacgact ggaccacgtc cgagcccacg
     4621 atatccctgc ccgaccagtc gcctcccttg acgaggacgt ccggccttat ccggctgatg
     4681 agctcgtagg gcgtgtcctc ctcgaacacg acgacgtaat caacgcatgc cagggcaccc
     4741 agcactcggc acctggcgtc gacggggttt acgggcatcg agggaccctt gagcctcctg
     4801 accgaggcgt ccgagttgag gcccaccacg aggcagtcgc cgaggcggcg cgccccctcg
     4861 aggtaggaga cgtgcccctt gtgcaggata tcgaaacacc cgttggtaaa gacgatcttc
     4921 atacccctgt ccctcatggc gtcgatccgc ccggccaggt cctcgatgcc tatggggcaa
     4981 gggcgttgcc cgatctcggg ggggaaaccc ctcctcgcca tgcgggaggt gtaccacccg
     5041 agcgcgagat aggagggaag gatgtcgggg aagcatctct cgagcgcatc cacgtgcatc
     5101 ctcaccgtgt cccagtcgcc gcgggacacg gggcccgtca aggcggcccc gcccaggcgc
     5161 tcgatgttcg cgagggtccc ccgcacgagc cccatgaggt gggcacggtc gaggccgatc
     5221 ctgcccgcca ccgccgaggc ctcgtccaaa agcgccacga ggtagttgga ggcgatcacc
     5281 gcggaggtgt ggtagaggag cttgtcctcg ggcctgatat ccaccatgcg g
//
LOCUS       DAOSCR010000113         5307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9788_length_5307_cov_3.061691, whole genome shotgun sequence.
ACCESSION   DAOSCR010000113 DAOSCR010000000
VERSION     DAOSCR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9788_length_5307_cov_3.061691"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PLT69_04155"
     CDS             <1..743
                     /locus_tag="PLT69_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13666.1"
                     /translation="TWDKAMASLEELAGLGCPIVVFEGGEPLLWSDGTRSFADIALAA
                     KKLFACVGVTTNGTVSLDVPTDIVWVSIDGTRETHDYLRSGSYDRAMGNILTSAHDRI
                     YVHMTLNSVNAGELETVAALASAMPQVKGLTLQLFYPYHRGEEDLALGPEARRRVLEE
                     ALRLKRAGYPILNSAWGLGVMRDNSWRCRQFLLANVETDGRVYTGCYAGHRAEVDCSA
                     CGFTPVAEASGAYDLVPGALAAGFRIFA"
     gene            complement(1377..2855)
                     /locus_tag="PLT69_04160"
     CDS             complement(1377..2855)
                     /locus_tag="PLT69_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ13667.1"
                     /translation="MSRWPKNPRVYEINTAAWLSGLGSRYQRAVDLSSVPAQEWNAVA
                     ELGMDAVWLMGVWQRSPLSRKRALEAKGVLPEMERALSDLVPEDVIGSAYSVRRYEVD
                     ERFGGQAGLSTARKELAKRGVMLVLDFVPNHTAVDHPWVVSHPEFYVRGSPQDIVETP
                     GLFTELDGGVFAYGKDPYFPAWTDVLQLNAFSGAVRKETVEILRGIAGLCDGIRCDMA
                     MLVMNDVFERTWGEHAGHAPDRDFWEEVIPSVKEIFPDVLFMAEAYWDLEWALMEQGF
                     DCCYDKRLYDRLLCGNAHSVRLHLGADPAYQERLVRFLENHDERRCAEAFPPGRLEAA
                     AVAVTTLPGVRLFHEGQFEGRRVRLPVQLARGPLEPLDGRLKGFYSRLLKVTRTPVMR
                     EGRWELCGITGWPDNPTCASLLAWDWVLGPQRRLVVINFSDRPAQGLVKPNGLESGPG
                     ELELADLMGGGEFRVKLRDAAGEGIYVDLGPWGYHVMSVLNA"
     gene            complement(2901..5255)
                     /locus_tag="PLT69_04165"
     CDS             complement(2901..5255)
                     /locus_tag="PLT69_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ13668.1"
                     /translation="MRARAVCVFVLCAAALAALASVLKTEDRLPEGGGRAAATDDAQT
                     GCRACHGEFYRRWAHSAHGRTYEPYSDEAARRFPGPRVQDIGTAQAAYTLDLRPGKGY
                     ATEKGSFPWSGVKRKIDFILGVRRVIFLFSKGESGRFEALPFCYDVPAGKWLDIEKCG
                     VAHALAGRRSGEGRLVVFDTGCRSCHIGPDPDFYELRTDSYRELPKNPGISCSACHGD
                     TAGHAEAARAGRPTAPGKAGMVVVRGLDPSRRTDLCTACHSTTRPLTAAYKPGQPLFD
                     HFDPVTLEDPAVPADGRVTGGTAVFTSWLANPCARSRRLDCTYCHEPGGGFRFRDKAR
                     VNDACLACHKDKAKRIEEHTHHKASSAAGACITCHMALPAVGGGGRADHSMLPPMPAL
                     SSSAKVPNGCNACHLEGDPRWAQGWVRAWYRRDFQAPYLERAKLVEDARAGEWSRLDE
                     MVSLVASNTRSELYAASLLRLLNTCPSPSKEKALRPAMKDPSPLIRASAADGLSEGVS
                     RAAADDLQALANDPVRLVRVRAAAAAARIKTVPVSRPGSGDMAKASGEYISSLMARPD
                     RWTSHVDMGVHFLERGDARGAILSFKAASKLEPRACEPHVNLAMAYAALGDANAAETE
                     LKEALRLDPANAPALFNMGLLMNQKGEKDEAVRYLKEAFKNDPGMAEAAYNLAMLVAE
                     KNPKEAVYWARRAYQARTDIKYAFALAYFLKQDGDWEEAIGTARGIVARDPSHLDAYL
                     LMGEIYEKKGRPLDAEKVYRQAIAAPGMSEEGRKRLEKKLKAFR"
BASE COUNT          854 a         1858 c         1690 g          905 t
ORIGIN      
        1 tgacctggga caaggccatg gcgtcgctcg aagagctcgc cgggctcggc tgcccgatcg
       61 tcgtgttcga gggcggtgag cccttgctct ggagcgacgg cacccgctct ttcgccgaca
      121 tcgcgctcgc ggccaaaaag ctcttcgcct gcgtcggggt taccaccaac ggcacggtga
      181 gcctggacgt ccccacggac atcgtgtggg tgagcatcga cggcacgcgc gagacacacg
      241 actacctgcg gtcgggatcc tacgacaggg cgatggggaa tatcctcaca tcggcccacg
      301 atcggatcta cgtgcacatg accctgaaca gcgtaaacgc cggcgagctc gagacggtgg
      361 ccgctctggc cagcgccatg ccccaggtga aggggctgac cctccagctg ttctacccgt
      421 accacagggg cgaggaagac ctcgccctgg gccccgaggc ccgcaggcgc gtgctcgagg
      481 aggccctgag gctgaagagg gcgggctacc ccatcctcaa ctccgcatgg gggctgggcg
      541 tcatgagaga caactcgtgg aggtgcaggc agttcctcct cgccaacgtg gagaccgacg
      601 ggagggtgta caccgggtgc tacgcgggcc acagggcgga agtggactgt tcggcgtgcg
      661 ggttcacccc ggtggccgag gcgtcggggg catacgacct ggtccccggc gcgctcgcgg
      721 cagggttcag gatcttcgcc tgagagcctg ggggatttcc ccggacaacc tgggggctgc
      781 agacggcctg gccgcgatgg ttcacgaccg ggatcctcct cgagcccggc agcgcctaca
      841 gccaagcaag cgcccaggac aaagggggcg gggttgtggg attccggcct gggccccgcc
      901 ccgagacccc ggggtctcta gtcctagaaa gcagcgaggg ctcgtgatct tgtcctgatc
      961 acgcagcggg tttcccggtt tccccgtgcc gttgtctcga gaagggccgg ccaggcactc
     1021 cgggaggcca gcacacaaac gaaaagggca ggcgactcag tcgggccggc cagtcacccc
     1081 gggaggccgg gctcaaggtg atgcaaagag accccgggca tccttcgcgc cttcgtgtcg
     1141 ggcggcctca gcggttttac gggtgcatgc gacgcccatg cccccctttc ggtatccaga
     1201 ggaagcgtcc ggaatgcccc ttcgcccaat ggagcccccg cagcctccca cgcctcacat
     1261 gcccgaatga tcgcccgtcg ccccttgtcg gcgccccccc tcaggtgcag gctttgacca
     1321 tggctccaga gtgcatgccc gaatgatcgc ccgtcgcccc tcgccagcgc ccccggtcag
     1381 gcgttcaaga cgctcatcac gtgatagccc catgggccaa ggtcgacata gatcccctcc
     1441 cctgccgcgt cacggagctt gacccggaac tcgccaccgc ccatgaggtc cgcaagctcg
     1501 agttccccgg ggccgctctc gaggccgttc ggcttcacca gtccctgcgc gggcctgtcc
     1561 gagaagttga tcaccaccag gcgcctctgt gggccgagaa cccagtccca ggcaaggagg
     1621 gaggcacagg tcgggttgtc gggccagccg gtgatcccgc acaactccca cctgccttca
     1681 cgcatgaccg gcgtgcgggt gaccttgagc agccgcgagt agaaaccctt gagacgcccg
     1741 tcgagaggct ccagcggccc ccgtgcgagc tggacgggca gacgcactct cctcccctcg
     1801 aactgcccct cgtggaagag gcgcacgccg ggcagggtgg tcaccgccac ggccgccgcc
     1861 tcgagcctgc ccggcgggaa ggcctcggcg cagcgccgct cgtcatggtt ctcgaggaag
     1921 cggacgagcc tctcctggta cgcagggtct gcaccgagat gcagcctgac ggagtgggcg
     1981 ttcccgcaga ggagcctgtc gtagagacgc ttgtcatagc aacagtcgaa cccttgctcc
     2041 atgagggccc actcgaggtc ccagtatgcc tcggccatga agagcacgtc cgggaagatc
     2101 tccttgaccg aaggtatcac ctcttcccag aagtccctgt caggggcgtg gcctgcatgc
     2161 tccccccagg tacgctcgaa cacgtcgttc atcaccagca tggccatgtc gcaccgtatc
     2221 ccgtcgcaaa ggcccgcgat ccccctgagg atctcgacgg tctccttccg gaccgcgccg
     2281 ctgaaggcgt tgagctggag tacatccgtc caggccggga agtacgggtc cttcccgtac
     2341 gcgaagacac ccccatcgag ctccgtgaag aggccggggg tctcgacgat gtcctgcgga
     2401 gagcccctca cgtagaactc cgggtggctc accacccagg ggtgatcgac ggcggtatgg
     2461 ttcggcacga aatcgagcac gagcatgacc cccctcttcg cgagctcctt gcgtgccgtt
     2521 gagagcccgg cctggccgcc gaaacgctcg tccacctcgt atcgcctcac gctgtaggct
     2581 gacccgatca catcctccgg gaccaggtcc gagagggccc tttccatctc gggaagcacg
     2641 cctttcgcct cgagggcccg cttcctggac agcgggctcc tctgccagac gcccatgagc
     2701 cataccgcgt ccatgccgag ttcggcgacg gcgttccact cctgtgcggg caccgacgag
     2761 agatccactg cccgttggta cctggaaccg aggcccgaca gccaggccgc cgtgttgatc
     2821 tcgtacacgc gggggttctt cggccacctg ctcaactccc agctcctctc aggtatcggc
     2881 ggacgcctgt cccgccatct tcaccggaag gccttcagct tcttctcgag gcgcttcctg
     2941 ccctcttcgc tcatccccgg ggcggcgatc gcctgccggt agaccttctc ggcatccagg
     3001 ggcctcccct tcttctcgta gatctctccc atgagaaggt atgcatcgag gtgcgagggg
     3061 tctctcgcga cgatccccct tgcggtgccg atcgcctctt cccagtcgcc gtcctgcttg
     3121 agaaagtatg cgagggcgaa ggcgtacttg atgtctgtac gcgcctggta tgccctcctc
     3181 gcccagtaca cggcctcctt ggggttcttc tcggccacga gcatcgcgag gttgtatgcc
     3241 gcctcggcca tgcccgggtc gttcttgaag gcctccttca ggtacctcac cgcctcgtcc
     3301 ttctcgccct tctggttcat gagcaggccc atgttgaaaa gggccggggc gttcgccggg
     3361 tcgagcctca gcgcctcctt cagctccgtc tcggcggcat tggcgtcgcc aagggccgca
     3421 taggccatgg cgaggttcac gtggggctcg cacgccctcg gctcgagctt ggaggcggcc
     3481 ttgaaggaga ggatcgcgcc gcgtgcatcc ccccgctcga gaaagtggac gcccatgtcc
     3541 acgtgggagg tccacctgtc cggcctcgcc atgagggacg agatgtattc cccggaggcc
     3601 ttggccatgt ccccggagcc gggccgagag accggcaccg tcttgattct cgctgcggct
     3661 gccgcggccc tcacgcgaac gaggcgtacc gggtcgttcg ccagtgcctg gaggtcgtcc
     3721 gccgcggccc gggagacgcc ttccgagagc ccatccgctg cggatgcccg gatgaggggc
     3781 gacgggtcct tcatcgcagg cctcagggcc ttctccttgg aagggctcgg gcacgtgttc
     3841 aagagcctca gcagggaggc ggcgtacagt tcgctcctcg tgttcgaggc aacgagtgac
     3901 accatctcgt cgagcctcga ccactcaccg gcacgggcgt cttcgacgag tttcgcacgc
     3961 tcgagatacg gcgcctggaa gtctctcctg taccatgccc tcacccagcc ctgggcccac
     4021 ctcgggtcgc cctcgaggtg acaggcgttg cacccgtttg gaaccttggc cgacgaggag
     4081 agggcgggca tcgggggcag catggagtgg tccgcccttc cgccgcctcc cacggcgggc
     4141 agggccatgt ggcaggtgat gcacgcgccc gcggcagacg acgccttgtg atgcgtgtgc
     4201 tcctcgatcc tctttgcctt gtccttgtgg cacgcaaggc aggcatcgtt caccctggcc
     4261 ttgtccctga agcggaagcc gccgcccggc tcgtggcagt atgtgcagtc gagcctgcgc
     4321 gagcgtgcgc acggattggc gagccacgac gtgaacacgg ccgtgccgcc tgtgaccctc
     4381 ccgtccgcag gtacggcagg gtcttccagg gtgacggggt cgaagtggtc gaacaggggc
     4441 tggcctggtt tgtacgcagc agtgaggggc ctcgtcgtgg aatggcaggc cgtgcacaga
     4501 tcggttctcc tcgaggggtc cagcccacgg accaccacca tgccggcctt gcccggggcg
     4561 gtgggcctcc cggctcgtgc agcctccgcg tggcccgcgg tatcaccgtg acaggcggag
     4621 cacgagatgc cggggttctt gggcagctcc ctgtaggagt cggtcctgag ctcgtagaaa
     4681 tccgggtccg gccctatgtg gcacgagcgg cagcccgtgt cgaacacgac caggcgcccc
     4741 tctccagacc ggcggccggc aagcgcgtga gcgacaccgc acttctcgat gtccagccac
     4801 ttcccggcag gcacgtcgta acagaagggc aacgcctcga agcggccgga ctctcccttc
     4861 gagaagagaa agatcacacg ccttacgccc aggatgaagt ctatcttgcg cttgacgccg
     4921 gaccagggaa acgagccctt ctctgtcgcg tacccctttc cgggcctcag atccagggtg
     4981 tacgccgcct gggcggtgcc gatgtcctgg accctcggac cgggaaacct ccgggccgcc
     5041 tcgtccgaat acggctcgta cgtcctcccg tgggccgaat gcgcccagcg cctgtaaaac
     5101 tccccgtgac aggcccggca ccctgtctgt gcatcatccg tcgcggccgc ccggccgccg
     5161 ccttcaggca accggtcttc ggtcttcagg acactcgcta aagcggcgag ggccgcagca
     5221 cacagcacga agacgcagac cgctctcgct ctcatgacca ccgggaccct cctccatgca
     5281 ccaattccac caccgccatt gtatcag
//
LOCUS       DAOSCR010000114         5305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9797_length_5305_cov_2.288571, whole genome shotgun sequence.
ACCESSION   DAOSCR010000114 DAOSCR010000000
VERSION     DAOSCR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9797_length_5305_cov_2.288571"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1642
                     /locus_tag="PLT69_04170"
     CDS             185..1642
                     /locus_tag="PLT69_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ13669.1"
                     /translation="MTIALRSIHKHYGDIRANDGIDLVLRPSTVHGILGENGAGKSTL
                     MKILSGYVRKTSGTILVDSEEVDFKNPAEAASHGIGMLYQDPLDFPPLTVLENFMAGQ
                     RMKAWPDRSLFRKRLAGLCREMGFDLDPETPVVRLTVGERQQLELARHLAAGRRVLIL
                     DEPTTGISSLQKDVLFSALRRLSSGGATVVLVSHKLEDVETLCDEVTVLKKGRVSGSA
                     VRPFSTGALLEMMFGREPAPPLRKGHEPGAQALFLEGVHAHGGRTGLAQCTVKVNRGE
                     VVGLAGLEGSGQDVFLRVAAGLTRPTRGRVHVSRKDMTGRDHHAFKTMGVAFQPAARL
                     EEGLIAGLSVAEHCAILERSSFSLDLDASASLAQERIQSFQVAARPESPVESLSGGNQ
                     QRLLLSFLPDDPVLLLLENPTRGLDRESVRWVWERLDAIASNGAAIVFSSSEIDEILM
                     VSDRVLVFFEGRIIHDVRPEDTDALMLGKAIAGRV"
     gene            1639..2718
                     /locus_tag="PLT69_04175"
     CDS             1639..2718
                     /locus_tag="PLT69_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ13670.1"
                     /translation="MRTVFARMGRPLEMGLKTALAGVAEIAASVLVVVAFTTLVLLVA
                     GAPPLDTFAVLVKGSLGSWSKLSHVIKVWIPLTLASCGLLFTFKAGLWNIGVEGQVMM
                     GAVATTLAMRMGGPSPLAGMVTALALVAGMAGGALWALCAGFLKTKAGVHEIFAGLGL
                     NFVSQGLVLWLIFGPWKQPGIASMSGTETFDERLWIPSIEALNLSPAALGMAVAAVIV
                     TAYLLSSTRLGLYLRAVGGNEKAAVLFGLKPHAVMTAAMLCAGGLAGLAGAFQVSGVY
                     HRLIPAISSGYGYLALLVVLLAYYNVWAAPVVAFFFACLNTGAIQLPMLLSLDSSLSG
                     VIQGSMVIAALAVHSVSTRSRRSRG"
     gene            2718..3701
                     /locus_tag="PLT69_04180"
     CDS             2718..3701
                     /locus_tag="PLT69_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ13671.1"
                     /translation="MMDGFDMLLVASAVIAGAAPLVMATLGETLTERAGVVNLSLDGT
                     IVLSAMAAFVVASLSGSLLAGFAVSAAVGACVAALVALFSVRLGMSQVAVGFVLTLMT
                     RDLAYFLGSQYARTEGPISGSAPVPVLSGMPVLGQVLFNHSVPVYLSMIMVAFAWWYL
                     YRTSWGLRLRCVGENMEAAYARGIDPDLVRVVYTVAGGALVGLSGAAFSLCTKPGWGQ
                     PQGCEGSGWIALALVIFGAWHPVRAALGAYLFAFLQVMSIHFQSWFPDVPAQVFQVAP
                     FPLMIFLLAVSTLVHSEEAKMLAERHPLLRILTRGLAQAPPLSLGKARSRD"
     gene            complement(4142..4600)
                     /locus_tag="PLT69_04185"
     CDS             complement(4142..4600)
                     /locus_tag="PLT69_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13672.1"
                     /translation="MSRWLALMAAVLVPLTSHGMTLLNDSALDLVYSQAGISINPDVT
                     LSIHVDVIAWGDEDGLEPGPYNPWGIDTGPGYIGVKDLRIENVRVRLRTDPNDHYGGY
                     DAATMCKPMTIDVGTRTAGDTSQTFVRLAPGALQVTAEEAGFTVTLGSQP"
     gene            complement(4950..5078)
                     /locus_tag="PLT69_04190"
     CDS             complement(4950..5078)
                     /locus_tag="PLT69_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13673.1"
                     /translation="MAQGKVTRRQKDISRLIKERGRSAKERARQERGKRPVKPERP"
BASE COUNT          804 a         1678 c         1827 g          996 t
ORIGIN      
        1 cagccaggca aagtaggcgc ccgcgaaccg tgggaggacc acttgccgcg acccgcaccg
       61 cgtgggtcat gcgcggacgc ttccctcatg gggccatgct cgggaacacg gcggcgcgtc
      121 cgacggcgag ggcggccggg cccccgccgg gggtgtggcg ggccgcccgc gcacggttgt
      181 ccacatgacg atagcactga gatcgataca caagcactac ggagacatcc gcgcgaacga
      241 cgggatcgac ctcgtcctca ggccctcgac cgtacacggc atcctcggcg agaacggcgc
      301 cggcaagagc acgctcatga agatcctgtc cggctacgta cgcaagacga gcgggaccat
      361 cctcgtggac tccgaggagg tggacttcaa gaacccggct gaagccgcat cccacggcat
      421 cggcatgctc taccaggacc cgctggactt tcccccgttg accgtgctcg agaacttcat
      481 ggcagggcag cgcatgaagg cctggcctga caggtcactg ttcaggaaga ggcttgccgg
      541 cctgtgccgc gagatgggct tcgacctcga ccccgagaca cccgtggtca ggctgaccgt
      601 gggggagagg cagcagctgg agctcgccag gcacctggcg gcgggcagga gggtcttgat
      661 cctcgacgaa ccgaccacgg ggatctcctc gctccagaag gatgtgctgt tctcggcgct
      721 caggaggctc tcctcgggag gggcgaccgt ggtgctcgtg tcgcacaagc tcgaggacgt
      781 ggagaccctg tgcgacgagg tgaccgtgct caagaagggc agggtttcag gctctgcggt
      841 tcgacccttc tccacgggtg cactgctgga gatgatgttc ggcagggaac cggcgccccc
      901 cctgcgcaag gggcacgagc cgggggcgca ggccctcttt ctcgaggggg tccatgctca
      961 cgggggcagg accggccttg cccaatgcac ggtgaaggtg aacaggggcg aggtggtggg
     1021 acttgcgggt ctcgagggaa gcgggcagga cgtgttcctc cgggtggcag ccgggctgac
     1081 gcggccgacc aggggcaggg tccacgtctc gagaaaggac atgacgggaa gggatcacca
     1141 tgccttcaag acgatgggcg tggctttcca gcccgcggcc aggctggagg aggggctgat
     1201 cgcggggctc agcgtggcgg agcactgcgc gatcctggaa aggtcttcgt tctcccttga
     1261 cctggatgca tccgcgtccc tggcgcaaga aaggatccag agcttccagg tggcggccag
     1321 gcccgagagc cccgtggagt cgctctcagg cggcaaccag cagagactcc tcctctcctt
     1381 cctccctgac gaccccgtgc tcctgctcct cgagaacccg acgcggggtc tcgacagaga
     1441 gtctgtcagg tgggtctggg aacgactcga cgcgatcgcc tccaacggcg cggcgatcgt
     1501 cttctcgtct tccgagatcg acgagatcct catggtgtcc gacagggtgc tcgtcttctt
     1561 cgaggggagg atcatacacg acgtccgtcc cgaggacacc gacgccctca tgctcgggaa
     1621 ggccatcgcg gggagggtgt gaggacggtg tttgcaagga tgggtcgtcc cctcgagatg
     1681 ggcctgaaga cggcccttgc aggtgtcgcg gagatcgcgg cgagcgtgct ggtcgtcgtc
     1741 gcgttcacca cgctcgtgct cctcgtggcg ggcgccccgc cgctggacac cttcgccgtg
     1801 ctcgtcaagg gttctctggg gtcgtggtcc aagctgtcgc acgtgatcaa ggtgtggatc
     1861 cctctcacgc tggcgtcgtg cgggcttctc ttcacgttca aggcggggct ctggaacatc
     1921 ggggtcgaag gacaggtcat gatgggggcg gtcgccacga ccctggcgat gagaatgggc
     1981 ggcccatcgc cgcttgcagg catggtcacg gcgctggctc tcgtggcggg tatggccggc
     2041 ggggcgctgt gggcgctgtg cgccgggttc ctgaagacga aggcaggggt gcacgagatc
     2101 ttcgccgggc tcggcctcaa cttcgtctca cagggcctgg tgctgtggct catcttcggt
     2161 ccgtggaagc agcctggtat cgcctccatg agcgggacgg agacgttcga cgaaaggctc
     2221 tggatacctt ccatcgaggc cctcaacctc tcgcccgcag ccctgggcat ggccgtcgcc
     2281 gcggtgatcg tgaccgccta cctgctgtcc tccacccggc tggggctgta tttgcgcgcc
     2341 gtgggcggga acgagaaggc tgccgtcctg ttcggactga aaccccatgc cgtgatgacc
     2401 gcggcgatgc tctgcgccgg cggtcttgca gggctcgccg gcgccttcca ggtctcaggc
     2461 gtgtaccacc gcctcatacc cgccatctcg agcggttacg gctacctcgc cctgctcgtc
     2521 gtgctgctcg cctattacaa cgtctgggcc gcgccggtgg tggccttctt cttcgcgtgc
     2581 ctgaacaccg gggccatcca gctccccatg ttgctctccc tcgactcgtc cctgagcggg
     2641 gtgatccagg ggtccatggt gatcgcggcc ctcgccgtac actcggtgag cacccgctcg
     2701 aggaggtcca gggggtgatg atggacggct tcgacatgct cctcgtggca tcggccgtga
     2761 tcgctggcgc cgcgccgctg gtgatggcaa ccctcggcga gaccctgacc gagagggccg
     2821 gggtcgtcaa cttgtcgctc gatgggacca tcgtgctcag cgccatggcc gcgttcgtgg
     2881 tcgcatctct ctccgggagc ctgcttgcgg ggttcgccgt ctcggccgcc gtgggggcct
     2941 gtgtggccgc gctcgtcgcc ctgttctcgg tgcgcctggg gatgtcgcag gtggccgtgg
     3001 ggttcgtcct cacgctcatg accagggatc tcgcatattt cctcgggagc cagtacgcca
     3061 ggaccgaagg ccccatttct gggtctgcgc ccgtgccggt cctctcgggc atgcctgttc
     3121 tcggacaggt gttgttcaac cacagcgtgc cggtctacct gagcatgatc atggtggcat
     3181 ttgcctggtg gtacctctac cgtacttcct gggggcttcg tctcaggtgc gtgggggaaa
     3241 acatggaggc ggcctacgcc agggggatcg acccggacct ggtccgcgtc gtctacacgg
     3301 tcgccggggg cgccctggtg gggctctccg gggcggcgtt ctccctgtgc acgaagccgg
     3361 gctggggcca gccgcagggc tgcgaggggt caggctggat cgcgctcgcg ctcgtgatct
     3421 tcggggcgtg gcaccctgtg agggcggcgc tcggcgccta cctgttcgcg ttcctccagg
     3481 tcatgagcat ccacttccag tcgtggttcc cggacgtgcc ggcccaggtg ttccaggtgg
     3541 cgccgtttcc cctcatgatc ttcctcctcg ccgtctcgac cctcgtccac agcgaggagg
     3601 cgaagatgct tgccgagagg caccctctcc tgaggatcct cacgagaggt ctcgcacagg
     3661 cgccccccct gtccctggga aaggcgagga gcagggactg acaccccgca tccaggcaca
     3721 gaggaccctc cggtgcagcc ttgggccttc tctgcgtcac gatgccggag gtttccaggg
     3781 agtcacccgc ggtcacgtct cgaaggggac gcccgatgcc tcccaagcac tgaacacttc
     3841 aggtggttgc ccggagaagc gggtcttccc caagacggga gatgggtgat cgagacgggg
     3901 ccaggggcac ggcctcaggc acccccggcg tggatgcgct cgggggggaa cggggaagaa
     3961 agagggcgga caagatatat tccccagctg tcttggtatc cagcttccat caggaaggcg
     4021 gaagaatcca aagacgcctt tgacggggga gatatacgca tcggccgtct ctgtatccac
     4081 gccccaaggc ttgtctcggg gtgtcgtccc gcacgcgtga aggggacgag cttggggcag
     4141 ttcagggctg actgcccagg gtcacggtga acccggcctc ctcggcggtg acctggaggg
     4201 ccccaggcgc gaggcgcacg aaggtctggg aggtgtcccc cgccgtgcgg gttcccacgt
     4261 cgatggtcat gggcttgcac atggtggcgg cgtcgtatcc gccgtagtgg tcgttcgggt
     4321 cagttctgag tcgtacccgg acgttctcga tcctcaggtc cttcacgcct atgtaccccg
     4381 gtcctgtgtc gatcccccag gggttgtacg gcccgggctc gagcccgtcc tcgtcacccc
     4441 aggcgatcac gtcgacgtgt atgctcaacg tgacgtctgg gttgatggaa atgccggcct
     4501 gggaatagac gagatcgagc gcggaatcgt tcagcaaggt catgccgtgg ctggtcagcg
     4561 gaacgaggac cgctgccatg agagccaacc accgtgacat gccgtttcct ccgtgtcgaa
     4621 tgccctgtcc tgcccggtgc gccgtggcgc ctgtatgccg ggcccagtcg tgttcacatg
     4681 ataagatcat tgcggcgagg ttgcaagtgc agagtccctc tcagaggaca ggaagggggc
     4741 gcccctttca ggccgccatg gaggactccc atgggtgtcg gtgccgtggc cctcgatcgt
     4801 cgtgaacgtg ctgcagcggg tagggcgcca ttgggcggga tgcgtgccag ggcgcggaaa
     4861 ccagggacaa ccggggtcat gacctctcgg ccgtaacggg tcgtcccgtg tcgtgcagga
     4921 gaggtcggtc tttcctgcac acggctttgt catggccttt caggcttgac cggcctcttg
     4981 cccctttcct gccgggccct ttccttcgca gacctgccgc gttccttgat gaggcgggat
     5041 atgtccttct ggcgcctggt gaccttgcct tgagccacga cagcctcccg tgcccgttac
     5101 cttcggccct cctcccgtgc tgcgcggtta cgtgagagac ctttcctggt gtgaggcagg
     5161 gccatgtggt atggggtcta cagcacgttc tccatgttga tgaggtactt ggcggcgacc
     5221 gcgttgacag agcatgcagc ctttgcgcct gccaggtgtg atctcaagga ctcgaggtcg
     5281 gacttccagg ccaggtctcc aggag
//
LOCUS       DAOSCR010000115         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9852_length_5284_cov_3.905527, whole genome shotgun sequence.
ACCESSION   DAOSCR010000115 DAOSCR010000000
VERSION     DAOSCR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9852_length_5284_cov_3.905527"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLT69_04195"
     CDS             complement(<1..622)
                     /locus_tag="PLT69_04195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ13674.1"
                     /translation="MVRVFSTTPRIVMGPSSVKTLGAEVRKRGATRVLVVTDKGVIQA
                     GLLKPIEDSLKASKIKFQVFDKVEPDPRYEIVAGCVETARQARAQMLIGLGGGSPMDI
                     TKTAAVMITNKGPIGAYFGIDLVPKPGIPTILIPTTAGTGSEVTPIAILSDEGEKLKK
                     GIVSPYLYPSVGILDPELTIGLTPAVTAATGMDALIHAIEAYTSINA"
     gene            complement(669..1973)
                     /locus_tag="PLT69_04200"
     CDS             complement(669..1973)
                     /locus_tag="PLT69_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ13675.1"
                     /translation="MRDWHDIYKSKLVSAEEAVSNIKSGDRVVVGHASGSPEVLLKAM
                     VENRKAYKDVEIVHMVSMGVTEYCQPGMERHFVHNSLFAGASSRKAIHDGRAFYTASH
                     FSQIPRLFVERILPVDVTLCMLSTPDEHGYCSFGVSVDYTKPAAESSRLVIAEVTPHM
                     PRTLGDAFIHVSDIDFIVECDSRPIVLNPPRITDIDEKIGGYCAELIKDGDCLQLCIG
                     AVPDSILGFLKDKKDLGIHTEMFSDGVVDLVEAGVVTCSRKNFNPGKMVATFFMGTEK
                     LYRFVHNNPMVQMYPVHITNNPAIVAKNDNMVSINSTLQVDLTGQAASESIGYKQFSG
                     TGGQADFIRGAAWSKGGRSILAFHSTASGGKISRIVPHLDEGAVVTTTRTDIHFVVTE
                     YGIADLRGKSVPERAKALISIAHPDFRSDLKREFQRIYNRKV"
     gene            complement(1979..2275)
                     /locus_tag="PLT69_04205"
     CDS             complement(1979..2275)
                     /locus_tag="PLT69_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13676.1"
                     /translation="MKVGIKYCGGCRAGYDRVALVNRLKNDVAGAVDFVGPDDPEAEM
                     VLFVSGCKSACTSIDQIRGRAVRFVTSEEDAEHWIREVLENIQAEIGRIQARGR"
     gene            complement(2272..3732)
                     /locus_tag="PLT69_04210"
     CDS             complement(2272..3732)
                     /locus_tag="PLT69_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HOJ13677.1"
                     /translation="MGKLMNAKEYEESLRKLKLNVYMFGRKVENPVDDPIIRPSMNAV
                     AATYEFAHRPEYEDIMTAKSHITGKKINRFTHIHQSVDDLVKKSKMGRLLGSLTACCF
                     QRCVGMDALNALSITTHAIDAKYNTEYYKRFLKYLEYVQENDLVCDGAMTDPKGDRSL
                     APSKQFDPDLFMHVVEERPDGIVVSGAKAHQTGAVNSHEIIVMPTITMREEDKDYAIS
                     FALPSDTEGITYIMGRQSCDTRKLEGMTFDRGNMHYGGHEALVVFDKVFVPWERVFMY
                     KEYEFTTQLVENFAAYHRQSYACKVGVGDVLIGATQTIAEFNGTERASHVKDKIIEMN
                     HLNETLFCGCIACAAEGHREPSGTYLVDVLLANVHKQNITRFPYEIARLAQDIAGGLV
                     VTCPSAADLASPEVGKWVEKYLKTKPDVPTEHRLRILRLIENITMGTAAVGYLTESMH
                     GAGSPQAQRIMIARLVNMKQKQKMARFLCGIDSSGA"
     gene            4495..>5284
                     /locus_tag="PLT69_04215"
     CDS             4495..>5284
                     /locus_tag="PLT69_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOJ13678.1"
                     /translation="MRPGGLFLRLSSLTLTRVLLLWLAFLCVVATIVPQDPYPSLDGV
                     SFPVRIIRLLSLRDVFHSVWFLGGAVLLAANLSACLFSRMSRRAASSLHKMPSDFQEA
                     MLGQGGDPVKVSVSIRSALSGFGDTRLVEGSRVVVVSEKGSLRRLGPVIAHAGVFFVL
                     LGSAFAFAGFKGTIEIPEGETRNVVTLMDGSSRRLGFSVTCERFSLERYPNGMPKEYR
                     SEVVFTSQDGAIEKRRILVNHPAVYGGMLFSQSGYERRPIARITV"
BASE COUNT          854 a         1654 c         1635 g         1141 t
ORIGIN      
        1 tcgcattgat ggacgtgtag gcctcgatcg cgtggatgag ggcgtccatg ccggtcgccg
       61 ccgtcaccgc gggcgtcagg ccgatggtga gctcagggtc gaggatcccc acggaggggt
      121 agaggtacgg gctcacgatg cccttcttaa gcttctcccc ctcgtcggaa aggatggcga
      181 tcggggtcac ctcgctgccg gtgcccgccg tggtggggat caggatcgtg gggatccccg
      241 gtttcgggac gaggtcgatg ccgaagtatg cgccgatggg gcccttgttg gtgatcatga
      301 ccgccgcggt cttcgtgatg tccatgggac tgccgccgcc gagcccgatg agcatctgcg
      361 cgcgggcctg cctggcggtc tcgacgcagc cggccacgat ctcgtagcgg ggatccggtt
      421 cgaccttgtc gaacacctgg aacttgatct tcgatgcctt cagtgagtcc tcgatcggct
      481 tcaggagacc cgcctggatg acccccttgt ccgtcaccac gagcacgcgg gtcgcacccc
      541 gcttcctcac ctccgcgccc agggtcttca ccgaagaggg tcccatgacg atcctgggtg
      601 tggtgctgaa cactctcacc atgtccgtat cctccctcag tattctcttc cccgctcggg
      661 gcgaggttct ataccttccg gttgtagatg cgctggaact ccctcttgag atccgacctg
      721 aagtccgggt gggcgatcga tatgagcgcc ttcgccctct ccggcaccga ctttccgcga
      781 aggtccgcga tgccgtactc ggtcaccacg aagtggatgt ccgtgcgggt ggtggtgacc
      841 accgctccct cgtccaggtg ggggacgatc ctcgatatct ttccccccga cgcggtggag
      901 tggaacgcga ggatcgacct gccgcccttg gaccaagcgg ccccgcggat gaagtccgcc
      961 tggccgccgg tcccgctgaa ctgcttgtac ccgatggact cggatgcggc ttgcccggtc
     1021 aggtccacct ggagggtgga gttgatggag accatgttgt cgttcttcgc gacgatggca
     1081 gggttgttcg tgatgtggac cgggtacatc tggaccatgg ggttgttgtg cacgaacctg
     1141 tagagcttct cggtgcccat gaagaacgtg gcgaccatct tgcccgggtt gaagttcttg
     1201 cggctgcagg tgaccacgcc ggcctccacg aggtccacca cgccgtcgga gaacatctcg
     1261 gtatggatgc cgaggtcctt cttgtccttg agaaaaccga ggatggagtc cgggacggcg
     1321 cctatgcaga gctggaggca gtccccgtcc ttgatgagct ctgcgcagta cccgccgatc
     1381 ttctcgtcga tgtccgttat cctcgggggg ttgagcacaa tgggcctgga atcgcactcc
     1441 acgatgaagt ctatgtccga gacgtggatg aacgcatcac cgagcgttct cggcatgtga
     1501 ggggtcacct cggcgatcac cagccgggaa ctctccgccg cgggcttcgt gtagtccacc
     1561 gacaccccga aggagcagta accgtgctcg tccggggtgg agagcatgca cagcgtcacg
     1621 tccacgggaa ggatcctctc cacgaacagc ctcgggatct ggctgaagtg cgaggccgtg
     1681 tagaaggccc tcccgtcgtg gatcgccttc ctcgatgatg cgccggcgaa aagggaattg
     1741 tgcacgaagt gccgttccat gcccggctgg cagtactcgg tgacccccat cgagaccatg
     1801 tggacgatct cgacatcctt gtaggccttg cggttttcca ccatggcctt cagcaggacc
     1861 tccggtgagc cgctcgcgtg gcccacgacc accctgtccc cgctcttgat gttgctgacc
     1921 gcttcctcgg cgctgaccaa tttgctcttg tagatgtcgt gccagtctct caccggactt
     1981 acctcccccg tgcctgtatg cggccgatct cggcctgtat gttctcgagc acctccctga
     2041 tccagtgctc cgcgtcctct tcgctcgtga cgaaccgaac cgcccggccc ctgatctggt
     2101 cgatgctcgt acaggcgctt ttgcaaccgc tcacgaacag gaccatctcg gcctccgggt
     2161 cgtccgggcc gacgaaatcg accgctccgg ccacgtcgtt cttcagtctg ttcacgagcg
     2221 ccaccctgtc gtacccagct ctgcaaccac cgcagtactt gatcccgacc ttcatgcccc
     2281 gctgctgtcg atgccgcaca ggaacctggc catcttctgc ttctgcttca tgttcacgag
     2341 cctcgcgatc atgatcctct gggcctgcgg ggagcccgcc ccgtgcatgg actcggtgag
     2401 gtagccgacg gcggcggtgc ccatggtgat gttctcgatg agcctgagga tcctgagcct
     2461 gtgctcagtg ggcacgtcgg gcttcgtctt gaggtacttt tccacccact tgcccacctc
     2521 cgggcttgcg aggtccgccg cggacgggca cgtcacgacg aggcctcccg cgatgtcctg
     2581 cgcgagcctc gcgatctcat aggggaagcg cgtgatgttc tgcttgtgca cattcgcgag
     2641 cagcacgtcc accaggtacg tcccgctcgg ctcgcggtgc ccctctgccg cgcacgcgat
     2701 gcagccgcag aagagtgtct cgttgaggtg gttcatctcg atgatcttgt ccttgacgtg
     2761 ggaggcgcgc tcggtgccgt tgaactcggc tatggtctgc gtggcgccga tgaggacgtc
     2821 gcccacgccc accttgcagg cgtagctctg ccggtggtag gccgcgaagt tctcgaccag
     2881 ctgcgtcgtg aactcgtact ccttgtacat gaacacgcgt tcccacggca cgaacacctt
     2941 gtcgaacacc acgagggcct cgtggcctcc gtagtgcatg ttgcccctgt cgaaggtcat
     3001 gccctcgagc ttcctcgtgt cgcaggactg gcgccccatg atgtaggtga tcccctcggt
     3061 gtcgctcggc agcgcgaacg atatcgcata gtccttgtct tcctcgcgca tggtgatggt
     3121 gggcatcacg atgatctcgt gggagttgac cgcgcccgtc tggtgcgcct tggcgccgct
     3181 cacgacgatg ccgtccggcc tttcctcgac cacgtgcatg aagaggtccg ggtcgaactg
     3241 cttcgagggg gcgagcgagc ggtcgccctt cgggtcggtc atggcgccgt cgcagacgag
     3301 gtcgttttcc tggacgtact cgaggtactt gaggaacctc ttgtagtatt ccgtgttgta
     3361 cttggcgtct atcgcatggg tggtgatgga aagggcgttg agggcatcca tgcccacgca
     3421 cctctggaag cagcaggcgg tgagcgaccc cagcagcctg cccatcttgc tcttcttcac
     3481 gaggtcatcc acgctctggt ggatgtgggt gaaccggttg atcttcttgc cggtgatgtg
     3541 cgacttcgcg gtcatgatat cttcgtattc cggccggtgc gcgaactcgt aggtggcggc
     3601 gaccgcgttc atggacggcc tgatgatggg gtcatcgacc gggttttcca ctttcctgcc
     3661 gaacatgtac acgttcaact tcagtttcct caggctctcc tcgtactctt tcgcgttcat
     3721 gagctttccc ataacgtcct ccgctcgtca gtgatctttc cggcgtctcg atgccagatg
     3781 cgggatatac cagagacccc cgtttcctcc cttaccgaac aaccacccaa tcaatatgca
     3841 tgccacaggc acaagcatac gggtcatgca gagaaaagcc attggcttca agtgactacg
     3901 aggcataagc cgccgccggc gccgggcttt cccgggacgc ctcagcgggg aaccgatgca
     3961 cacgcgcaac gcatttttcg gtgagatggt gtaacatgtt aatatcaagc gcggtatcga
     4021 acatcacggg gccgatgcac gattgcaata ttcccgtgcg acccggacgt ccgtaatcta
     4081 taacctcagg aaataattct ccgaacacgg ccgaggacgg tttgtgttgc accgatgcaa
     4141 cggcgctcgt acatccccca acagcccgcg ccgaacctgt gaaagccgcc tgggctgcca
     4201 agacatatcc gccaggagga aggcgaggtc atcccatgaa gatgaccctt cccgcatgag
     4261 tccttgctgc agaggcccgg tggctcgatg ggcggtcctt ggggtgttgc ccgtcgagca
     4321 cgaaaacctt tggtctttgc cctcggtcgc gcaggtggta aggtggtctc tgtatgcacc
     4381 cttgttcggg gagaccgaag ggccgagccg ccatgacgag gacctgccgg ggtttttccc
     4441 cgggagcggt ggagggtcaa agggcgtgat ctgggaggtg gtgaagggat gaccatgaga
     4501 ccgggaggcc tctttttgcg gctctcgagc cttacgctca cccgggtgct cctcctgtgg
     4561 ttggccttct tgtgcgtcgt ggccacgatc gtaccccagg acccgtatcc cagcctcgac
     4621 ggagtgtctt tccccgtgag gatcatcagg ctcttgagcc tcagggacgt gttccactcg
     4681 gtgtggtttt tgggcggcgc cgtgctcctt gcggccaacc tgtcggcgtg ccttttttcc
     4741 cgcatgtcga gaagggccgc gtcctctttg cacaagatgc cctcagactt ccaggaggcg
     4801 atgcttgggc agggggggga tccggtgaag gtctccgtgt ccatcaggag cgctttgtcc
     4861 ggcttcggag acacccgcct cgtcgagggt tcccgggtcg tggtcgtaag cgagaagggc
     4921 tcccttcgca ggctgggccc cgtgatcgcc cacgcgggtg tctttttcgt cctcctgggc
     4981 tcggccttcg cctttgccgg cttcaaggga acgatcgaga tccccgaggg tgagacgcgt
     5041 aatgtcgtaa ccctcatgga cggctcgtcc aggaggctcg gcttctcggt cacgtgcgag
     5101 cggttctcgt tggaacgata cccgaacggc atgcccaagg agtacaggtc tgaggtggtc
     5161 ttcaccagtc aggacggcgc catcgagaag cggcggatcc tcgtgaacca cccggcggtc
     5221 tacggcggca tgctgttttc gcagagcggg tacgaaaggc ggcccatagc ccggatcacg
     5281 gtca
//
LOCUS       DAOSCR010000116         5283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9856_length_5283_cov_3.271423, whole genome shotgun sequence.
ACCESSION   DAOSCR010000116 DAOSCR010000000
VERSION     DAOSCR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9856_length_5283_cov_3.271423"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLT69_04220"
     CDS             complement(<1..739)
                     /locus_tag="PLT69_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13679.1"
                     /translation="MSRVKASGIKMHADVLDRLETMTLPQVLIHQAQAFGSDPPAIRE
                     KAYGIWQTYTWQDYLDYVRLTAYGLKALGLKRHDNVAMVVNNHPEWLFCQLGSQAMGA
                     VTLNLFTSAVASEIASTLKRVNARIVIVQDQEQTDKILEQSSNLPHLEALVYVDPTGM
                     RGYAGNRLLVSYKDLLELGKKHELRHPGLFVEEVSKGKPSETAHMILTSGTTGVSKLA
                     MLSHRNYTAMARQWIETAPVTRGGNWLS"
     gene            complement(750..1556)
                     /locus_tag="PLT69_04225"
     CDS             complement(750..1556)
                     /locus_tag="PLT69_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13680.1"
                     /translation="MSAKRADLDIRDLSLSFGGIQALREVSLSVRTGELVAVIGPNGA
                     GKTSLMNCITGFYKPSSGSILFNGRDITSLPSHRLTSLGIGRTFQNIELFPGMTVLDN
                     LLLARHTKATYSFASACLFLRSVKREEVMHREILEEIIDFLEIQSIRKELVGSLPYGL
                     RKRVELGRALALEPSLLVLDEPFAGMTLEEKEDMVRFLLELHDGWGQTMILVEHDMSI
                     VMSISERVFVLNFGEKLAEGRPDEIQDHPDVIRAYLGETSDLEHDLPSGE"
     gene            complement(1626..2843)
                     /locus_tag="PLT69_04230"
     CDS             complement(1626..2843)
                     /locus_tag="PLT69_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ13681.1"
                     /translation="MVNVRRALMVGGIALFVCLTISSALHAKDIKVGAAINLTGPAST
                     WGQYHAKGTQDYFRYVNEVKGGVKGDKISLITVDTAYKPAEAVAAVRKFAMQEKVDMI
                     ATWSAGEGLQAKPLLQEYKIPAINYSTAWEILQPPVDFMYLPFGSYRLDCAAVMEYIS
                     AVHKGKSPAKVGLLTYNNAYGRSIHEPSKEFAKGKNIEIVSIEEFPPQTVDLTTELLR
                     LKQSGAEYVFMQMLPAAIISTLKAADRIGYNPVFIGTWTSTDPDFFKNGQGLIRNRVK
                     MHFCGGLPSDRTPGVKLMETIWKRYKTVDSFDTAYWEGIVVGMIMERAFLRAKEKFGE
                     VKPINVNKALESFKNEDFGGLVPAVTYSGTDHEASFTGRIVQIHENGTFTPLTEFFTP
                     GKGKIKLVGTKTK"
     gene            complement(3106..4008)
                     /locus_tag="PLT69_04235"
     CDS             complement(3106..4008)
                     /locus_tag="PLT69_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13682.1"
                     /translation="MEIMRPPEHLVIRPPSEARSLLVRLVRGCNWNRCAFCGIYDFFD
                     TPFSERSLEEVIEDIDALRAIYGDIFKTAFLGDANPLHLKTDFLVQVLEHLRKTFPRL
                     ERVTSYARASSLAKKTREELVSIRKAGLDRVHVGMESGSDKVLRFHMKGTTQAQLIDA
                     GHKAMSSGMELSYYVLLGLGGVELWEEHAKESARVLNEVKPHFARIRRLWIHPMSRLG
                     EEIRSGRFTEQTPEGTVLELRSILAELEPSPMVLTCDHANNYIQVEGRIDLHKDGMLE
                     VIDSFLSLPEEQRIRHYRSVPSVI"
     gene            4473..>5283
                     /locus_tag="PLT69_04240"
     CDS             4473..>5283
                     /locus_tag="PLT69_04240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ13683.1"
                     /translation="MTQMISPQRPMKIALIGNFQPRQCGIATFTTDLHTALEQENRGG
                     QCLAVVMNDVPEGYPYPPQVRFEINQRVLRDYLMAAEFLNISGVDVACLQHEYGIFGG
                     DYGVYILDLLENLRMPVVSTLHTVLKEPNPWQLAVVKRLAQLSDRLVVMSNKARQILR
                     EVYAVPEHKTVMIHHGIPDVPFVDPNYYKDQYGVEGRKVILTFGLLSPGKGIETMIEA
                     LPLVVKNHPDAVYIVLGATHPHVRRDQGESYRLSLQRLARGLGVGDHVIFHN"
BASE COUNT          936 a         1579 c         1683 g         1085 t
ORIGIN      
        1 tggagagcca gttgccgccc ctggttacag gcgcggtctc gatccattgc ctggccatgg
       61 cggtgtagtt gcggtgcgac aacatggcga gcttcgagac cccggtggtg cccgaggtga
      121 ggatcatgtg cgccgtctcg ctcggctttc ccttggagac ctcctcgacg aagagcccgg
      181 gatgcctgag ttcgtgtttc ttcccgagct cgaggaggtc tttgtagctg acgaggagcc
      241 tgtttcccgc gtacccgcgc atacccgtgg ggtccacgta gacgagcgcc tcgaggtgag
      301 gcaggttcga cgactgttcg aggatcttgt cggtctgctc ctggtcttgc acgatcacta
      361 tcctggcgtt gacccgcttc agcgtggagg cgatctcgct cgccacggcc gacgtgaaga
      421 ggttgagcgt gacggcgccc atggcctggg acccgagctg gcagaacagc cactcggggt
      481 ggttgttcac gaccatggcc acattgtcgt gacgcttgag gcccaacgcc ttgaggccgt
      541 acgcggtgag cctcacgtag tcgaggtagt cctgccaggt gtatgtctgc cagatcccgt
      601 aggccttttc cctgatcgcc ggcggatcgc tcccgaaggc ctgcgcctgg tggatgagca
      661 cctgcggcag ggtcatggtc tcgagcctgt cgagaacatc cgcgtgcatc ttgatgccgc
      721 tcgctttcac ccgggacatc gcctcccccc tactcccccg aggggagatc gtgttccagg
      781 tcagaggtct ccccgaggta tgctcggatg acgtccgggt ggtcctggat ctcgtccggt
      841 cgaccctcgg cgagcttctc gccgaagttg agcacgaaga cgcgctcgga aatgctcatg
      901 acgatggaca tgtcgtgttc cacgaggatc atggtctggc cccagccgtc gtgaagctcc
      961 aggaggaagc ggaccatgtc ttccttttcc tcgagggtca tccccgcgaa gggttcgtcg
     1021 aggacgagca ggctgggttc cagggcgagg gccctgccga gctccacgcg ctttcggagg
     1081 ccgtagggaa gcgagcccac gagctccttc ctgatcgact ggatctcgag gaagtcgatg
     1141 atctcttcca ggatttcccg gtgcatgact tcctccctct tgaccgagcg gaggaacagg
     1201 catgcggagg cgaaggagta cgtcgccttg gtgtggcggg cgaggagcag gttgtcgagc
     1261 acggtcatgc cggggaagag ctcgatgttc tggaacgtcc tgccgatgcc gagcgaggtg
     1321 agcctgtgcg acggcaggga cgttatgtcc ctgccgttga acaggatgct gcctgatgag
     1381 ggtttgtaga agccggtgat gcagttcatg aggctcgtct tcccggcgcc gttcgggcct
     1441 atcacggcca cgagttcccc ggtgcgcacg gagagggata cctccctgag ggcctggatg
     1501 ccgccgaatg acagcgagag gtctcgtatg tcgagatccg ccctcttggc ggacatcttc
     1561 tcgtgggtga agattcgtgg ttgccctcgg tatgccctca caatgctctc ccgcgcgttc
     1621 cctcgctatt tcgtcttggt gcctacgagc ttgatctttc ccttgcccgg ggtgaagaac
     1681 tccgtgagcg gggtgaacgt gccgttctcg tggatctgga cgatcctccc ggtgaacgac
     1741 gcctcgtggt cggtgccgga ataggtgacg gcgggcacga ggccgccgaa atcctcgttc
     1801 ttgaacgatt cgagggcctt gttcacgttt atgggtttga cctcgccgaa cttctccttc
     1861 gccctaagga aggcgcgttc catgatcatg ccgaccacga tcccctccca gtatgcggtg
     1921 tcaaaggagt cgacggtctt gtaccgcttc cagatggttt ccatgagctt cacgccgggc
     1981 gtcctgtccg agggaagccc gccgcagaag tgcattttca cccggtttct gatgaggccc
     2041 tggccgttct tgaagaaatc gggatcggtg gacgtccagg tcccgatgaa cacggggttg
     2101 tagccgatcc ggtctgcggc cttgagcgtg ctgatgatgg ccgccggcaa catctgcatg
     2161 aagacgtact ccgcgccgct ctgcttgagg cggagaagct cggtggtgag atcgacggtc
     2221 tgcgggggga actcctcgat ggagacgatc tcgatgtttt tcccctttgc gaactccttg
     2281 ctcggctcgt ggatggacct gccgtaggca ttgttgtagg tgaggagccc caccttggcc
     2341 ggggacttgc ccttgtgcac ggcggagatg tactccatga ccgcggcgca gtcgagcctg
     2401 tagctgccga acggcaggta catgaagtca acgggcggct ggaggatctc ccacgccgtc
     2461 gaatagttga tcgcagggat cttgtattcc tggaggagcg gctttgcctg cagcccctcg
     2521 cctgcgctcc aggtggcgat catgtcgacc ttctcctgca tggcgaactt cctcaccgcg
     2581 gcgaccgcct ctgcgggttt gtatgccgtg tcgacggtga tcagggagat cttgtccccc
     2641 ttcacgccgc ccttgacctc gttcacgtag cggaagtagt cctgggtgcc cttggcatgg
     2701 tactgtcccc aggtggaagc gggacccgtg aggttgatcg ccgccccgac cttgatgtcc
     2761 ttggcatgaa gcgccgagga gatcgtcaag cagacgaaca gggcgatacc tcccaccatc
     2821 aaagcacgtc tcacgttcac cattgcatgt cctccttgtc ttttgcgtta acttcctcgg
     2881 cacgacgtac ctgctcgtgg tgccgccggg atctctgtgc atacaaaaaa gccgcaggtc
     2941 gacgtacaag gtccacctgc ggcttttttt gactaggtct cggagctaca agcgcaggca
     3001 gacctaatca aaaaagaaga acccgaacat ggtgcctggt ctcgcaattg ccataggata
     3061 gaggcattaa ggaaatatcg gggagaagtc aaggaaaaag tcgcctcaga tgaccgaggg
     3121 gaccgaacgg tagtgcctga tcctctgttc ttcgggaagg gagaggaacg aatcgatgac
     3181 ctcgagcatc ccgtccttgt ggaggtcgat gcgcccttcc acctggatat agttgttcgc
     3241 gtggtcgcag gtgagcacca tgggcgaggg ctcgagctcc gcgaggatgc tcctgagctc
     3301 gagcaccgtt ccctcggggg tctgttcggt gaatcgcccc gaccggatct cttccccgag
     3361 ccttgacatg gggtggatcc agagcctcct gatgcgggcg aagtggggct tgacttcgtt
     3421 gagcacgcgg gccgactcct tggcgtgctc ctcccagagc tcgacgcccc cgagcccgag
     3481 gaggacgtag tagctcaatt ccatccccga ggacatggcc ttgtgcccgg cgtcgatgag
     3541 ttgtgcctgc gtcgtcccct tcatgtggaa cctcaagacc ttgtcggagc cgctctccat
     3601 ccccacgtgg accctgtcga gccccgcctt gcgtatcgag acgagttcct cccgggtctt
     3661 cttcgccaga gatgacgccc gggcgtacga ggtgacccgt tcgagccgtg ggaaggtctt
     3721 gcgcaggtgc tcgagcacct gaacgaggaa gtccgtcttg aggtgcaggg ggtttgcgtc
     3781 gcccaagaac gcggtcttga agatgtcgcc gtagatggcg cgcagggcgt cgatgtcctc
     3841 gatgacctcc tccagggagc gctcggaaaa gggcgtgtcg aagaagtcgt agatcccgca
     3901 gaaggcgcac ctgttccagt tgcaccctct cacgaggcgg acgagcaggc ttctggcctc
     3961 gctcggtggt ctgatcacca gatgttccgg gggtctcatg atctccatat aagcactcgc
     4021 tctcctctgg tcaaggaatc gcaagggtgc tcgcaaagcc gccccccttg tctccgtgcg
     4081 gttcatgccc tatgagaaga ggaggcgagg aacagccgcc atcgacagat cctcgcgcag
     4141 tacgaggagg cgcggcaaga cacccggcac aacgagcgtt ctgggagatt tcggtcaatg
     4201 ggacggaatg ttctgggatc gagtcgtgtg ggcgttagaa gtacgtcgtt cgagcctgag
     4261 gcacgtggaa ccttttcatc cctcggcctc cctgcggcct tcgcgggaga ggccaaggcc
     4321 cgcctgtcgc ccgcccgggg cgcgacacac acagacgcat cctcggttct tgatggtcgt
     4381 ggatcgcctc ggtcaggcaa ggggaatcca tgccccccga ccgtgctgga tggcgagccg
     4441 gggcctttcg agggatcgga caggaggaga gcatgacgca gatgatatcg ccgcagcgcc
     4501 cgatgaagat agcgttgata gggaacttcc agccgcgaca gtgcggcatc gccaccttca
     4561 ccacggacct ccacacggcc ctcgagcagg aaaaccgggg aggccagtgc ctggccgtcg
     4621 tcatgaacga tgtcccggag gggtatccct acccgcccca ggtaaggttc gagatcaacc
     4681 agcgggtgct cagggattat ctcatggctg cggagttctt gaacatcagc ggcgtggatg
     4741 tggcctgcct ccagcacgag tacgggatct tcggtggtga ttacggcgtg tatatcctcg
     4801 acctcttgga gaatctccgc atgcccgtcg tgagcacgct ccacacggtg ctcaaggagc
     4861 cgaacccctg gcagctcgcc gtggtcaagc gtttggccca gctctcggac cgcctggtgg
     4921 tcatgagcaa caaggcccgc cagatcctga gagaagtcta cgcggtgcca gagcacaaga
     4981 cggtcatgat ccaccacggt atcccggacg tcccgttcgt ggacccgaac tattacaagg
     5041 accagtacgg cgtcgagggc cgcaaggtga tcctcacctt cggcctcctc tccccgggaa
     5101 aggggataga gaccatgatc gaggcccttc cgctcgtggt gaagaaccat cccgacgccg
     5161 tgtacatcgt gctgggagcg acccaccccc acgtgcgcag ggatcagggg gaatcctaca
     5221 ggctgtcgct gcagcgactg gcgagggggc tcggggtggg cgatcacgtg atcttccaca
     5281 acc
//
LOCUS       DAOSCR010000117         5276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9866_length_5276_cov_3.039839, whole genome shotgun sequence.
ACCESSION   DAOSCR010000117 DAOSCR010000000
VERSION     DAOSCR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9866_length_5276_cov_3.039839"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PLT69_04245"
     CDS             <1..1129
                     /locus_tag="PLT69_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ13684.1"
                     /translation="PVIIMSGHGTIETAVKATRLGALDFVEKPLDLNKIIITVNNILH
                     LKDLEEENALLRHKVEKDDEIIGNSPAILRLKEQIDRVAPTDAWVLILGENGTGKELV
                     ARRIHKKSRRSSYPFVEVNCAAIPEELIESELFGHEKGAFTGATERKRGKFDLANNGT
                     LFLDEIGDMSMATQAKILRILQEQRFERVGGSQTIQVDVRVLTATNKNLEQEIAEGRF
                     RQDLYYRLNVIPLYVPRLVERREDIPLLVEHFIHLFTQDKGNRRKTITEGAVEKLMQH
                     DWPGNVRELKNIIERLVIMTPGDTITADDIFPLGVTATSDLDRILNMQELKDARAEFE
                     RIFIERKLALCGNNISKTADLIGVERSNLHRKIKSLGINVE"
     gene            1119..2675
                     /locus_tag="PLT69_04250"
     CDS             1119..2675
                     /locus_tag="PLT69_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13685.1"
                     /translation="MWSESGAIRKDKGGKLNVALVYPGTYHVGMSNLGFHQMYRVLNA
                     HPLILCERFFTDAPRSVESGRPLSDFHVVAFSVSYELDAVQAVRMLCANGMDPHAERR
                     TTGPLVMAGGAAVTMNPEPLAPACDICFAGDGEVIVQPLYEAFSKSSSREEFLERMLQ
                     TGGVYLPSRTRPVIEGGTLEAFEGPQVMRAVTETLDDPARTSVVTRDTAFGDMFLVET
                     ARGCPFSCSFCGARQIYSPYRCAGVDTVARILDEASRHRSKVGLVSTSLNTHPQAERL
                     FEEIAARGLKLAPPSLRSGILTKGLLGALEKSGVKGVTLAPETGSETLREALGKRIPD
                     ATILEDIRALVASGVRDIKLYFMVGLPGEGPRELDATVDFVKRVRQVFIQVSRGNRRI
                     GTVSVSIGTFVPKPGTPLERSPMITVDDARARIRRIEQGLKRESNVRVTHEGPKWSYL
                     QAVVARGDRRVHDLLVRLAGSDEQGWTAALKRWPVNPDYFALRPRDEDEVLPWSFISG
                     PPRGGACRGT"
     gene            2665..3363
                     /gene="radC"
                     /locus_tag="PLT69_04255"
     CDS             2665..3363
                     /gene="radC"
                     /locus_tag="PLT69_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ13686.1"
                     /translation="MERDDGRPRRPHHEGHRSRLRGRFRTSGPASLADYELLELLLGY
                     AIPRKDTKPIAKGLLERFKSLRRVLDTPAEQLEQYPGIGRQASTLIRLAKALMERYLE
                     PFDEAAPVLSRPERLADYLRTSMGGQARERFMILCLDASGRLVHRAVLSEGTVDGAYV
                     YPREVLRTAIESDAVSLVLVHNHPSGTLKPSEQDLRLTRELSEICSRVGITLNDHLVV
                     TARGIYSISLGREL"
     gene            3804..5171
                     /gene="rmuC"
                     /locus_tag="PLT69_04260"
     CDS             3804..5171
                     /gene="rmuC"
                     /locus_tag="PLT69_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOJ13687.1"
                     /translation="MEYSTAAIVALSLLAGLAAGFLVARARYTGALEASRSSLEELRA
                     REEALAAQVAQARDQLERERIEQAKNRVRLEEAQASLEQQRRLIEEASARLADTFKAL
                     SLDALHASGSAFMQMAQKAIEAVASDMKGDLEKRQAAVEAVVRPLEESLKRYEAQVQA
                     MEQSRQAAFGDISRHIGELVRMQERLADQTRSLATALKEPKARGRWGELTLRRVVELA
                     GMSAHCDFTEQVSTGTEDGRRRPDLVVTLPGNRRIVVDAKAPLDAFLAAYEAADPDSQ
                     NALLARHARSVKDHVQALSSREYWKQFTPTPDFVVLFLPGEQFFAAALEKDSSLIDDA
                     IAKKVLIATPVTLIALLRSVAMSWQEHGMQENARRVWETGRELFDRLRVFVGHLERMR
                     TGIESAAKAYNDAVGSFESRVVPSANRLKSLTAPGVDDAACRLEPVGVSLRRPPEALP
                     DEADD"
BASE COUNT          955 a         1793 c         1766 g          762 t
ORIGIN      
        1 gcccgtgatc atcatgtccg gccacggcac catcgagacc gcggtcaagg ccacgcgcct
       61 gggcgcactg gacttcgtgg agaaacccct cgatctcaac aagatcatca tcacggtgaa
      121 caacatcctc cacctcaagg atctcgagga ggagaacgca ctcctcaggc acaaggtgga
      181 gaaggacgac gagatcatcg ggaactcgcc cgccatcctc aggctcaagg aacagatcga
      241 cagggtggcg cccaccgacg cgtgggtcct catcctgggc gagaacggca cgggcaagga
      301 gctcgtggcc cggcgcatcc acaagaagag caggaggagt tcctacccgt tcgtcgaggt
      361 gaactgcgcg gcgatccccg aggagctcat cgagagcgag ctcttcggac acgagaaggg
      421 cgcgttcacg ggcgcgaccg agcgcaagcg cggaaagttc gacctggcca acaacggcac
      481 gctcttcctc gacgagatag gcgacatgag catggctacc caggcgaaga tcctgcgcat
      541 cctccaggaa caacgcttcg agcgcgtggg ggggtcgcag accatccagg tggacgtgcg
      601 cgtgctcacc gcgacgaaca agaacctgga gcaggagatc gcggaaggga ggttcaggca
      661 ggacctgtac taccgcctca acgtgatccc cctgtacgtc ccgcgcctcg tggaacgccg
      721 ggaggacata cccctgctcg tggaacactt tatacacctc ttcacgcagg acaagggaaa
      781 caggcgcaag accatcaccg agggggccgt cgagaagctc atgcagcacg actggccggg
      841 caacgtccgg gaactcaaga acatcatcga acgcctcgtc atcatgaccc cgggtgacac
      901 catcaccgcc gatgacatct tccccctggg cgtcaccgcg acgagcgacc tcgaccgcat
      961 cctcaacatg caggaactga aggacgcgcg cgccgagttc gagcggatct tcatagaacg
     1021 caagctcgcc ctgtgcggca acaacatctc gaagaccgcc gatctcatcg gggtggagcg
     1081 gagcaacctc caccgcaaga tcaagagcct ggggatcaat gtggagtgag agcggcgcca
     1141 tacgcaagga caagggcggg aagctgaacg tcgccctggt gtaccccggc acgtaccacg
     1201 tggggatgtc gaacctcggt ttccaccaga tgtaccgggt tctcaacgcc caccccctca
     1261 tcctgtgcga gcggttcttc accgacgcgc cgagatcggt ggagagcggg aggccccttt
     1321 cggacttcca cgtcgtcgcc ttctccgtgt cgtacgagct ggacgcggtc caggccgtgc
     1381 gcatgctgtg cgcaaacggc atggaccctc acgcggagag gcggacgacg ggcccgctgg
     1441 tcatggccgg gggcgccgcg gtcaccatga acccggaacc gctcgccccg gcatgcgaca
     1501 tctgcttcgc aggcgacggg gaggtgatcg tccagccgct ctacgaggcc ttctcgaaga
     1561 gctcctcgcg tgaggagttc ctcgagcgca tgctgcaaac cggcggcgtg tatctcccct
     1621 cgaggacgcg cccggtcatc gagggaggca cgctggaggc cttcgagggg ccacaggtaa
     1681 tgcgagccgt cacggaaacg ctggacgacc ccgcgcgcac ctccgtcgtg acgagagaca
     1741 cggccttcgg cgacatgttc ctcgtcgaga cggccagggg gtgcccgttc tcctgctcct
     1801 tctgcggcgc caggcagatc tactcaccgt acaggtgcgc gggggtggac accgtggccc
     1861 gcatcctcga cgaggcatcc aggcacaggt cgaaggtggg cctcgtgagc acctcgctca
     1921 acacccaccc gcaggcggaa aggctgttcg aggagatcgc cgcgcggggc ctgaaactcg
     1981 cccccccctc gctcaggtcc ggcatcctga cgaaggggct cttgggggcg ctcgagaaat
     2041 caggggtcaa gggggtcacc ctcgcgcccg agaccgggtc cgagaccctg agggaggccc
     2101 tggggaagcg gataccggac gcgaccatcc tcgaggacat cagggcgctc gtggcatcgg
     2161 gcgtccgcga catcaagctc tacttcatgg tgggcctgcc gggcgagggg ccgagggaac
     2221 tggacgccac cgtggatttc gtcaagaggg tgcggcaggt cttcatccag gtctcccgcg
     2281 gcaacaggcg gataggcacg gtgagcgtga gcataggcac gttcgtgccc aagcccggca
     2341 cccccctcga gcggagcccc atgatcacgg tcgacgatgc cagggcccgc atcaggcgca
     2401 tcgagcaggg cctgaagagg gagtcgaacg tgcgcgtgac gcacgagggg ccgaagtggt
     2461 cgtacctgca ggccgtcgtc gccaggggcg acaggcgcgt ccacgacctc ctcgtgaggc
     2521 tcgccgggag cgacgagcag ggatggacgg ccgcgctcaa gcgctggccg gtgaaccccg
     2581 actacttcgc gctcaggccg cgggacgagg acgaggtgct cccctggtcg ttcatctcgg
     2641 gacccccccg tggaggtgcg tgccgtggaa cgtgacgacg gccggccgag gaggcctcac
     2701 cacgaggggc accgctcgag gctcaggggg cggttcagga cgtccggccc cgcttcgctg
     2761 gccgactacg agctcctcga gctcctcctg ggatacgcca tcccgaggaa ggacaccaag
     2821 cccatcgcca aggggctcct cgaaaggttc aagtctttgc ggcgggtgct ggacacgcct
     2881 gccgagcagc tcgagcagta cccggggatc ggccggcagg cctctaccct gatccgcctc
     2941 gcaaaggcgc tcatggaaag gtacctcgag cccttcgacg aggccgcacc cgtcctctcg
     3001 agacccgagc gcctggccga ctacctgagg acgtccatgg gcgggcaggc ccgcgagcgc
     3061 ttcatgatcc tgtgcctcga cgcctcgggc aggctcgtgc accgcgccgt cctctccgag
     3121 ggcacggtcg acggcgcgta cgtgtacccc agggaggtgc tgaggacggc catcgagtcg
     3181 gacgccgtct cgctcgttct cgtgcacaac cacccatccg gcaccctcaa gccctccgag
     3241 caggacctga ggctcacccg ggagctctcc gagatctgct cgcgcgtcgg catcaccctc
     3301 aacgaccacc tcgtggtcac cgccaggggt atctacagca tctccctcgg ccgcgagctg
     3361 tagaccgcgg gcggcaccgt cttgccccgt tcccgccgcc ttctcgatgg cgtcccctgc
     3421 gccctcttcc cgccatgccc tgcggcctcg cccctcgacg cgctcgtttc tgcccccctc
     3481 cctggagcat gcgcctgtac cccggcggtc gacatcccgg ggctggcgcg gagccctgcg
     3541 ccgccgcagc ggggcaggcg ccgacattca acgtgggtgc ctgcctccag ggggacacgg
     3601 gcatcttggg cgcctggttg cattgggtca ctcctgggcg cccggggcct ctcggtggag
     3661 cgcgacatgg cggctggtac gggctccgaa ggacggcagc ttcccccgag gcacccgcac
     3721 gatgcctcgg gccggtagac gcactgccct ttccggtcga gccggccctt gacatcgacg
     3781 cctcgagctg acatgggttt tgtatggagt attccacagc cgcaatcgtg gcgctgtcgc
     3841 tgcttgcagg cctcgccgcg ggattccttg tagcgcgcgc gaggtacaca ggcgcgctcg
     3901 aggcctccag gagctcgttg gaagaactca gggcgaggga agaggccctg gcggcccagg
     3961 tcgcacaggc cagggaccag ctcgagcgcg agcgcataga gcaggccaag aaccgggtac
     4021 ggctcgagga ggcccaggcg tctctcgagc agcagcgcag gctcatcgaa gaggcatccg
     4081 caaggctcgc ggacaccttc aaggcgctct ccctggatgc cctacatgca agcggctcgg
     4141 ccttcatgca gatggcacag aaggccatcg aggcggtggc ctcggacatg aagggcgacc
     4201 tcgagaagcg ccaggcagcc gtggaggccg tcgtgaggcc gctggaagag tcgctcaagc
     4261 gctacgaggc ccaggtgcag gccatggagc agtccaggca ggcggccttc ggcgacatct
     4321 cccgccacat cggcgagctc gtcaggatgc aggagaggct cgcggaccag acgaggtctc
     4381 tggccacggc cctcaaggaa cccaaggcga ggggccgctg gggggaactc accctcaggc
     4441 gcgtggtgga gctcgcgggg atgtcggccc actgcgactt caccgagcag gtgagcacgg
     4501 gcaccgagga cgggaggaga cggccggacc tcgtcgtgac cctgcccggc aacaggcgca
     4561 tcgtggtgga cgccaaggcc cccctcgacg cgttcctggc cgcctacgag gcggccgacc
     4621 ccgacagcca gaacgcgctc ttggcccgac atgcgcgttc cgtgaaagac cacgtccagg
     4681 ccctctcttc ccgcgagtac tggaagcagt tcacgcccac gccggacttc gtggtcctgt
     4741 tcctcccggg cgagcagttc ttcgccgcgg ccctcgagaa ggattcctcc ctcatcgacg
     4801 acgccatcgc gaagaaggtc ctcatagcca cgccggtgac cctcatagcg ctcctgaggt
     4861 cggtggccat gagctggcag gaacacggca tgcaggaaaa cgcgaggagg gtctgggaga
     4921 ccggccggga gctcttcgac cgcctcaggg tgttcgtggg gcacctcgaa cgcatgcgga
     4981 cgggcatcga gagcgcggca aaggcgtaca acgatgcggt cgggtccttc gagtcgaggg
     5041 tcgtcccgtc cgcgaacagg ctcaagtcgc tcaccgcgcc cggggtcgac gacgccgcct
     5101 gcaggctcga gcccgttggg gtttccctga gaaggccccc cgaggccctc ccggacgagg
     5161 cggacgactg agtcccgggg ccttgcgccc cgagagacgg acatgggggt gcgcgggagg
     5221 tacctgggga ccgggcctgc ctgcctcagg cccctgaccc caccacgggg gccggc
//
LOCUS       DAOSCR010000118         5243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9944_length_5243_cov_3.780069, whole genome shotgun sequence.
ACCESSION   DAOSCR010000118 DAOSCR010000000
VERSION     DAOSCR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9944_length_5243_cov_3.780069"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PLT69_04265"
     CDS             complement(<1..1482)
                     /locus_tag="PLT69_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ13688.1"
                     /translation="MTFFKNLRIATKLIAVGSILLIVPIAVVGYLAVKTSSKGLAELE
                     DEQLVAVATSLSQTVEHALTGELKLVKDLAVGNSTIKAASAVARSGIQGASAEIAELN
                     HKLKKFAETEGLKDNSQVVVVADTKGSIYAASSPTYIGISISDRQYFKDALSGKGNIG
                     EPNLNKATGTPFVPVAQPIYGDSGGVVGVIANILDIGFLTDLVSNTKTGKTGYAFMVD
                     GNGLTIAHPVKENIMKLNLAELEGMESITKKMLAGQSGVERYVFKGVAKTGGFAPVKM
                     TGWSIGLSLPDEEFLAPARTVRNIVLVVAVVAFAAVFAVFYLFARTISSAIRKGVDLA
                     VSVSEGDLTSDIDIHQNDEIGQLADALRNMIAKLRNIVTDVHNAADNVAAGAEQMSST
                     AEQMSQGATEQAAAAEEASSSMEQMAANIRQNADNALQTEKIAVKSAEDAREGGRAVE
                     ETVSAMKTIAEKIAIVEEIARQTDLLALNAAIEAARAGEHGKGF"
     gene            complement(1717..2094)
                     /locus_tag="PLT69_04270"
     CDS             complement(1717..2094)
                     /locus_tag="PLT69_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13689.1"
                     /translation="MAGTGLVMHLDGYLLKEALETEDSPILLACIRDDQPGHDIVQSL
                     GSVASQAGEVVRVCYITEELFSCVKDRFCIVGTPTFLLIHKGRLVDRLLGKATTLQIL
                     RFVSTGLARGALTNSQHERTNNP"
     gene            complement(2324..4042)
                     /locus_tag="PLT69_04275"
     CDS             complement(2324..4042)
                     /locus_tag="PLT69_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOJ13690.1"
                     /translation="MKNARILIIDDDEHVRKTFTEILSREGFVVKTASDCETALSLVE
                     ETPFDFILLDIVLGEHSGIDLLRELKLKGVYAPIIMITGRPDLENASASLRYGAFDYI
                     VKPIRSETLLRVTKNALQHKVLQDEKRFLEQEKERYRMHLECIFNSVEEAIIMVDTEL
                     RVIEANHAASRLCGISSENPRSLIEPASACGGACRQVLADAFAKNVGINEYRIECKGP
                     DGTDRAIVMSVSPIRAVDGLPSGAVMVLRDVTRIMTLERELKGRHQYHNIIGKSPRMQ
                     EVFRLLENLRDIETTVLVTGKSGTGKELVARAIHYGGARSSGPFIAVNCSALAENLLE
                     SELFGHVKGAFTGAIRDKAGRFELAHNGTLFLDEIGDIPPHIQLKLLRVLETKQIERV
                     GDATPIQVDVQVITATNRDLKDLVHRGLFREDLYYRLKVVEVHLPALTERREDIPLLV
                     DHFLALFRERFRKDISGVSQETMHLFMEYPWPGNVRELVHCMEHAFVMCRSHVIDTRD
                     LPPEIRTFRSGPGVQLDAASRSHLERDDILNALKTCGGNRARAARHLGVSRQTLYRKM
                     KEFGIP"
     gene            complement(4052..4657)
                     /locus_tag="PLT69_04280"
     CDS             complement(4052..4657)
                     /locus_tag="PLT69_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13691.1"
                     /translation="MDEATLSRIFEPFFTTKDPSRGTGLGLATVHGIVSSLGGAVLVT
                     SKPGQGSTFDVYLPAAPAASQSMQCTQPDIPTGSGQRILVVDDEPQILDIAASMLEQL
                     GYRCITRSSSMEAFEFFRSDPARIDLVITDQTMPGITGADLARAMLELRPDVPIILMT
                     GFSEVLGRDDALSLGIREYIEKPFTMGDLAQKVSSCLLEKE"
     gene            complement(4661..>5243)
                     /locus_tag="PLT69_04285"
     CDS             complement(4661..>5243)
                     /locus_tag="PLT69_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOJ13692.1"
                     /translation="TERKRIEQEHRLAEARLAQAQKLEAIGTLAGGIAHDFNNILVAI
                     IGYSELALKDLPEVSRARESIAEVLRAGLRARELIKQILTFSRRTETERTSVHIPSIV
                     KETIRLLRSSIPSTIAIVERTDPSVPHILADPTQIHQLILNLCTNAYHAMSPAGGTLS
                     IRVDSVDVDDRLASGHPELKTGPHVRLSVRDTG"
BASE COUNT          881 a         1609 c         1651 g         1102 t
ORIGIN      
        1 gaaccccttg ccgtgttcgc ccgcacgtgc cgcctcgatg gcggcgttca gcgcgagaag
       61 gtccgtctgg cgtgcgatct cttccacgat ggcgatcttc tccgcgatgg tcttcatcgc
      121 cgacaccgtc tcctccacgg ccctgccgcc ttcccgagcg tcctccgcgg acttgaccgc
      181 gatcttctcc gtctggaggg cattgtccgc attctgcctg atgttcgccg ccatctgttc
      241 catggaggac gaggcctcct cagcggcggc cgcctgctcc gtcgcgccct gcgacatctg
      301 ttcggccgtg gagctcatct gttccgctcc cgcagcgacg ttgtcagcgg cgttgtgcac
      361 atcggtgacg atgttcctga gcttggcgat catgttcctc aaggcgtcgg caagttgccc
      421 gatctcgtcg ttctggtgaa tgtcgatgtc ggaggtcagg tctccctcgg acacgctgac
      481 cgccagatcc acccctttcc ggatggccga ggaaatggtc cgtgcgaaga ggtagaacac
      541 cgcgaacacc gccgcaaacg ccacgaccgc gacgacgaga acgatgttcc gcacggttcg
      601 cgcgggcgcc aggaactctt cgtcgggcag ggacaacccg atgctccatc ccgtcatctt
      661 gaccggcgca aacccgcctg tcttggccac tcccttgaag acgtagcgtt ccacgccgct
      721 ctgtcccgcg agcatcttct tggtgatcga ttccatgccc tcgagctcag ccaagttgag
      781 cttcatgatg ttctccttga ccgggtgcgc tatggtgagg ccgttgccgt ccaccatgaa
      841 ggcgtatcct gtctttcccg tcttggtgtt ggagacgagg tcggtcaaga agccgatgtc
      901 gagaatgttg gcgatgacgc ccacgacgcc gcccgaatcg ccgtagatcg gctgcgcgac
      961 aggaacgaat ggcgtccccg tcgccttgtt caggttcggc tcgccgatgt tgcccttgcc
     1021 cgagagggcg tccttgaaat actgcctgtc ggagatcgag atgccgatgt acgtgggcga
     1081 gctggccgcg tagatggagc ctttcgtgtc ggcgaccacc accacctggg agttgtcctt
     1141 gagcccctcc gtctcggcga acttcttcag cttgtggttg agttccgcga tctcggccga
     1201 agccccctgg atgccggacc gcgccaccgc gctcgcggcc ttgatggtgg agttgcccac
     1261 ggcgaggtct ttcacgagct tgagctcgcc ggtaagcgcg tgctccaccg tctgcgacag
     1321 cgaggtcgca accgccacga gctgctcgtc ctcgagttct gcaagaccct tggacgaggt
     1381 cttgaccgcc aggtacccca cgaccgcaat gggcacgatg aggaggatgg accccacggc
     1441 tatcagtttc gtcgcgattc tcaggttttt gaagaacgtc atctttccct ccttggatgc
     1501 gttatggatt gcttcccccc tgaccgtcag cagggcggag acttgcctgc ggcgacccgg
     1561 tggactgtct catatcgtct tgcagaatga ccccattgct gcccgtccgc ctcatcccac
     1621 tcgttctcat ggtgaaaaaa cgccccctct ccgggcagga gagagggcat acaacgggtt
     1681 gggccatgat ctgcttgtgg gttgcgtgaa gggcgatcat gggttgttcg tcctctcgtg
     1741 ttggctgttc gtcagggcac ctcgcgcaag gcctgtcgag acgaaacgaa ggatctggag
     1801 tgtcgtcgcc ttgccaagca gccggtccac caggcgcccc ttgtggatga gcaggaatgt
     1861 cggggtcccc acgatgcaga agcggtcctt cacacaagag aagagctcct cggtgatgta
     1921 gcatacacga acgacttcac cagcctgcga ggcgaccgac cccagagact gaacgatgtc
     1981 atgccccggc tggtcgtccc tgatgcacgc caagagtatg ggcgagtcct ctgtctcgag
     2041 agcctccttg agcagatagc cgtccaggtg catgaccagg cctgtgcctg ccatcgtttc
     2101 tccttacaag tcagcagtat ggtggaacgt gcgggacagg aacaaacatc atgccagaag
     2161 ccacggatct ggccagtgcg gtaaatatct atttctatca atatgttgcg ggcttgccgg
     2221 cggtatcgaa atggtgatcg gggcaggatg catgtaacat gttacatgca gggtgtacca
     2281 cggaactgtc acatggtaca tgtgttccgt ttctgtttcc gtattacgga atgccgaatt
     2341 ccttcatctt gcggtagagc gtctggcggc tcaccccgag atgccgggcc gcccgcgccc
     2401 ggttcccgcc gcaggtcttg agcgcgttca ggatgtcatc ccgctcgagg tgcgagcggc
     2461 tcgccgcgtc gagctgcacg ccggggcccg agcggaacgt ccggatctcg ggcgggaggt
     2521 cccgggtatc gatgacatgc gaccggcaca tgacgaaggc atgttccatg cagtgaacga
     2581 gctcccgcac attccctggc catgggtact ccatgaacag gtgcatggtc tcctggctca
     2641 cgcccgagat atccttgcgg aatcgttccc tgaagagggc cagaaagtga tccacgagaa
     2701 gggggatgtc ttccctgcgc tcggtgagcg cggggaggtg cacctccacg accttgaggc
     2761 ggtagtagag gtcttcccgg aacaggccac ggtgcacgag atccttgagg tctcggttcg
     2821 tggccgtgat gacctggacg tccacctgta tgggcgtggc gtcccccacc ctctcgatct
     2881 gtttcgtctc gaggacgcgc agcagcttga gctggatatg gggcgggata tccccgatct
     2941 cgtcgaggaa gagcgtgccg ttgtgggcca gctcgaacct gccggccttg tcccgtatgg
     3001 cgcctgtgaa ggcccccttg acatggccga agagctcgct ttccaggagg ttctcagcca
     3061 gggcggaaca gttgacggcg atgaacgggc ccgaagaccg agcgcccccg taatggatgg
     3121 cacgcgccac gagctccttg cccgtgccgc tcttcccggt cacaaggacc gtggtctcga
     3181 tatccctgag attctcgagc aatcggaaca cttcctgcat cctggggctc ttgccgatga
     3241 tgttgtggta ctggtggcgt cccttgagtt cccgctcgag ggtcatgatc ctcgtgacat
     3301 ccctgagaac catgaccgcc cccgatggga ggccgtcgac cgccctgatg ggggaaacgc
     3361 tcatgacgat cgccctgtcg gtgccgtctg gccccttgca ctcgatgcgg tattcgttga
     3421 tgccgacgtt cttggcgaat gcgtccgcga gcacctgcct gcacgcgccc ccgcacgccg
     3481 atgcgggttc gatgagcgag cgcgggtttt ccgacgatat gccgcacaac cgggatgcgg
     3541 cgtggttcgc ctcgatgacg cgaagctcgg tatccaccat gatgatggct tcctctacgc
     3601 tgttgaatat gcactcgagg tgcatcctgt agcgttcctt ctcctgttcg aggaagcgtt
     3661 tctcgtcctg gagaaccttg tgctgaaggg cgttcttcgt caccctgagc agggtctcac
     3721 tgcggatggg cttgacgata tagtcgaagg ccccgtacct gagggaagcg gaggcgttct
     3781 cgaggtccgg cctgcccgtg atcatgatga tgggtgcgta cacccctttg agcttgagtt
     3841 ccctcagcag gtcgatgccg ctgtgctcgc ccaggacgat gtcgagcagg atgaaatcga
     3901 aaggggtctc ctccaccagc gagagcgctg tctcgcagtc ggacgccgtc ttcaccacga
     3961 acccctcgcg agacaggatc tcggtaaagg tcttcctcac gtgctcgtca tcgtcgatga
     4021 tcaggatgcg ggcgttcttc atggtcgatg gtcactcctt ctcgagaagg caagaactga
     4081 ccttctgggc caggtctccc atggtgaagg gcttctcgat atattcgcgt atgccgaggg
     4141 aaagggcatc gtcgcgcccc aggacctcgc tgaaccccgt catgaggatg atgggaacgt
     4201 cgggcctgag ctcgagcatt gcgcgcgcga ggtccgcgcc cgtgatgccc ggcatggtct
     4261 ggtcggtgat gacgagatcg atgcgtgccg gatcggacct gaagaactcg aacgcctcca
     4321 tgctggacga tcttgtgatg catcggtagc cgagctgctc gagcatcgag gcggcgatgt
     4381 ccaggatctg gggctcgtcg tcgaccacga ggatccgctg gccgcttccg gtggggatgt
     4441 ccggctgcgt gcactgcatc gattgggagg ctgcaggcgc ggctggaagg tagacgtcga
     4501 aggtgctccc ctgcccgggc ttggacgtca cgagcaccgc gccgccgagg gacgagacga
     4561 tgccgtgcac ggtcgccagc cccaggcccg tgcccctgga ggggtccttc gtggtgaaga
     4621 agggctcgaa gatgcggctc aaggtcgcct catccatgcc tcaccccgta tcccgcaccg
     4681 atagcctcac gtgcggcccg gtcttcagct cagggtgtcc ggacgccagg cggtcgtcca
     4741 cgtcgacgga atccacccgg atgctcagcg tgccgcccgc gggtgacatg gcgtggtaag
     4801 cgttcgtgca gaggttcagg atgagctggt ggatttgcgt tgggtctgcg aggatatggg
     4861 ggacagaggg gtcggtgcgc tccacgatgg ctatggtgct gggtatggac gacctcagga
     4921 gcctgatggt ctccttcacg atagagggga tgtgcacgga cgttcgctcc gtctccgtcc
     4981 ggcggctgaa ggtgagaatc tgcttgataa gctccctggc gcggagccct gccctcaaca
     5041 cctcggcgat gctctcgcgc gcgcggctga cctcggggag gtccttgagc gccagttccg
     5101 aatacccgat gatggccacg aggatgttgt tgaaatcgtg ggcgatgccc cctgccagcg
     5161 tgccgatcgc ctcgagcttc tgggcctgtg cgagcctcgc ctcggcgagg cgatgctcct
     5221 gttcgatgcg cttgcgttcg gtt
//
LOCUS       DAOSCR010000119         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9966_length_5234_cov_3.089593, whole genome shotgun sequence.
ACCESSION   DAOSCR010000119 DAOSCR010000000
VERSION     DAOSCR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9966_length_5234_cov_3.089593"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLT69_04290"
     CDS             <1..415
                     /locus_tag="PLT69_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ13693.1"
                     /translation="TAGGIMAEKKRILIIDDDRDYADALKIVLENNGFDVVHAPNAAE
                     GKSAIASSKPDLIILDVMMEKHTDGFDLCQDLKNEESCRMIPIIMVTAVTEKTGFKFS
                     PQTDGEYLPADDYVSKPVAVTELLARVNRLLASRG"
     gene            955..2931
                     /locus_tag="PLT69_04295"
     CDS             955..2931
                     /locus_tag="PLT69_04295"
                     /inference="COORDINATES: protein motif:HMM:NF028511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ13694.1"
                     /translation="MRLQIRLVISFLVATGITAAVASSVGFMMIGKTTLDEVQRKVQQ
                     DINTAKLVFRHSLERLENQLQFYAVRSPIHEAITKGNLEPLEDLRLLIRSDTFTSPGH
                     LALDMLTLVDTSGRVIFRAANPEAKGDVILWDSVVKRCLEEKRPVSSPILMPLDVIVA
                     QNPRLASRVEIPIVKTEKSVEIREKVLRQGMVLRSAYPILDRDGRLLGALVAGILLNK
                     DYSIVDTIKETVYQAERYRGKDMGFSTLFLGGVRIATNVMTRDNRRAVGTIVSKEVYD
                     RVILQGRDWYGRAFVVNDWYISAYTPIYDIDNKIIGMLYTGILEAKYRDMMMRTVFIF
                     LAVTALGMVIAFIISFRLGQSIVNRIKILKRATEAIAAGDLDYQLPSGASSGFDMLDE
                     AFNTMARSLKDRDERLQKAFQRITQTERLASLGQMAAGVAHEINNPLGGILLYSNLVL
                     EEMGPDDPSRDNMQKIIYQTERCKKIVQNLLDFARTPSGDMTPLSINEVIMTSLNLVK
                     DQSIFHGIEIVTELSEGLPTIMGDPSRLEEVFLNLFINASDAMCGKGRLRIASRVSST
                     GMVVITISDTGKGIDRAYLPHIFEPFFTTKEPGQGTGLGLSITYGIIQKHNGIIDVES
                     EPGRGTTFTITLPPTEGARDDRGEPHTAEEMKIG"
     gene            2924..4285
                     /locus_tag="PLT69_04300"
     CDS             2924..4285
                     /locus_tag="PLT69_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ13695.1"
                     /translation="MAKKSRRILIIDDEASIRDGCSQILERAGHEVESTGDAEYGLKL
                     AMSDRFDVILLDIRLPKIEGIEILRILKKEQMVQARIIVITGYGSIPLAVEAMRLGAV
                     NFLTKPFGAAELRGALNECFAEAADESSEDSLGMLIGSSDYMRELKETIRRVSKTDSS
                     VLITGESGTGKELVARTIHNLSRRSGMPFVAVDCSSLVPNLMESELFGHVKGAFSGAY
                     ESRQGRFQVAHRGTLFLDEISNISLDIQAKLLRVVQEQEVPRVGSSKPEKVDVRLITA
                     TNRDLRAEGESGRFREDLFYRISVVPMHIRPLREHRTDIMDIARHYLEIYRERHGAKV
                     RRFSIEAIQSLNSYSWPGNVRELKNTVERLCVLCDHEEVTLSDIMYYGQDPRGKAPVV
                     DPFSGRMRLVDVERDHIEKALRHFNFHMNKTAQFLGIDRKTLRLKIRTYGIKTPGNNE
                     KEE"
     gene            4713..5105
                     /locus_tag="PLT69_04305"
     CDS             4713..5105
                     /locus_tag="PLT69_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13696.1"
                     /translation="MESKKKILLIDDDKDFLMATKLILEKGGYEVFLAEDGKSGLEMA
                     RKVSPDLAIIDMMMETWSEGFNVVSRLRTSETGKQIPLIMLSAVDLQGPYQSFEPPEN
                     LPTVDLVQHKPIKADELLGHISTLLKKQ"
BASE COUNT         1133 a         1631 c         1600 g          870 t
ORIGIN      
        1 gacagcgggg ggtatcatgg cagagaagaa aaggatcctc atcatagacg acgacaggga
       61 ctacgccgac gcgctgaaga tagtgctcga gaacaacggt ttcgacgtgg tccacgcgcc
      121 gaacgcggcc gagggaaagt ccgcaatcgc ttccagcaag ccggatctca tcatactcga
      181 cgtgatgatg gagaagcaca ccgacggctt cgacctgtgt caggacctca agaacgagga
      241 gtcatgcagg atgatcccca tcatcatggt caccgcggtg acggagaaga cggggttcaa
      301 gttctcgccg cagaccgacg gcgagtacct gccggcggat gactacgtgt ccaagccggt
      361 cgccgtcacg gaactcctgg cgcgggtgaa caggctgctt gcatcgaggg gctgaggttt
      421 ctctcgggca cgtgacgaga aggcccccat atcgtcacgt gccctatggc cggccgcagg
      481 gcttctcctt cgggggcatc ctcttctccc cctgggatac cccgagggcc acgctcacgg
      541 gcgacccggt cacccggcgt gccggcatcg ctcgaggcga cccgggcagg ggccatcccc
      601 gaggcatccc gaaaacggcc gcatgtgttg ttcctgtgtc ggccggccga atgccggcaa
      661 ggatccatac accacccacc tcggtgatcg atgcggccat cgtcgtgatc gggcgcgcgg
      721 cagcagtcgg gatgcgctcc aggagccgtg agcctttgat ccccgcccca taccttctcg
      781 tgcccggacc gcccggatgg tccgaacaca tgcgtgcggc acaaagacaa gcatcgggtg
      841 tttcggggcc ttcggacgac gtatccgcag ggattcattg atcgagtccc gtcgagatgg
      901 tatagatggt gaaatgaagg cctactcttc gcaggtcatg gatgcgcatc ccctatgaga
      961 ttgcagattc ggctggtcat aagctttctg gtcgcgacag gcataacagc cgccgtcgct
     1021 tcaagcgtcg ggttcatgat gatcggcaag acgaccctcg acgaggtcca gcgcaaggtc
     1081 cagcaggaca taaacaccgc gaagctcgtg ttccgccaca gtctcgagag gctcgagaac
     1141 cagctgcagt tctacgcggt gcgctccccc atccacgagg cgatcaccaa gggcaacctc
     1201 gagcccctgg aagacctgcg cctcctgata cgcagcgaca ccttcacgtc acccggccac
     1261 ctggcgctcg acatgctcac cctcgtggac acgagcggca gggtgatctt cagggccgcg
     1321 aacccggagg cgaagggtga cgtgatcctg tgggactcgg tcgtgaagag gtgcctcgag
     1381 gagaagagac cggtctcctc gcccatcctg atgcccctgg acgtgatcgt ggcgcagaac
     1441 cccaggctcg cctcgcgggt ggagatcccc atcgtcaaga ccgagaaatc ggtagagatc
     1501 agggagaagg tgctgagaca aggcatggtg ctcagatccg cgtaccccat cctggacagg
     1561 gacgggaggc tcctgggcgc gctggtggca ggcatcctcc tgaacaagga ctactcgatc
     1621 gtggacacca tcaaggagac cgtgtaccag gccgaacgtt accggggcaa ggacatgggc
     1681 ttctccaccc tcttcctcgg gggtgtgagg atcgcgacga acgtcatgac gagggacaac
     1741 cgtcgggccg tgggaacgat cgtctccaag gaggtctacg accgggtgat acttcaaggc
     1801 agggactggt acggcagggc cttcgtggtg aacgactggt acatcagcgc ctacaccccc
     1861 atctacgaca tcgacaacaa gatcatcggc atgctctaca cggggatact cgaggcgaag
     1921 taccgcgaca tgatgatgag gaccgtgttc atcttcctgg cggtcacagc gctcggcatg
     1981 gtgatcgcct tcatcatctc gttcaggctc ggccagagca tcgtgaacag gatcaagatc
     2041 ctcaagcgcg ccaccgaggc catagccgcg ggcgacctcg actaccagct cccttccggt
     2101 gcatcctcgg gcttcgacat gctcgacgag gcgttcaaca ccatggccag atcgctcaag
     2161 gacagggacg agaggctcca gaaggccttc cagcggatca cccagacgga gcgcctcgca
     2221 tccctgggac agatggccgc gggcgtggcg cacgagatca acaaccccct gggcggcatc
     2281 ctcctctaca gcaatctcgt gctcgaggag atggggccgg acgacccgtc ccgggacaac
     2341 atgcagaaga tcatctacca gaccgaacgg tgcaagaaga tcgtccagaa cctgctcgac
     2401 ttcgcgagaa caccctcggg cgacatgacc ccgctgtcca tcaacgaggt gatcatgacg
     2461 tcgctcaacc tcgtcaagga tcaatccatc ttccacggca tagagatcgt gacggagctt
     2521 tcggaagggc tccccacgat catgggtgac ccgtcgcggc tggaagaggt ctttctcaac
     2581 ctcttcatca acgcctcgga cgccatgtgc ggcaagggca ggctgaggat cgcctcgcga
     2641 gtctcgtcga cgggcatggt ggtgatcacc atcagcgaca ccgggaaggg catcgacagg
     2701 gcctatctcc cgcacatctt cgaacccttc ttcacgacga aggagccggg ccagggtacg
     2761 ggcctcggcc tctccataac gtatggtatc atccagaaac acaacgggat catagacgtg
     2821 gaaagcgagc ccggcagggg gacgaccttc accatcaccc tgccccccac cgagggcgcc
     2881 agggatgaca ggggagaacc ccacaccgcc gaggagatga agattggcta agaaatcgcg
     2941 ccgcatactc atcatcgacg acgaggcgtc catccgggac gggtgcagcc agatcctcga
     3001 acgcgcaggc cacgaggtgg agagcaccgg tgacgcggag tacgggctga agttggctat
     3061 gagcgaccgt ttcgacgtca tcctcctcga catcaggctt cccaagatcg agggcataga
     3121 gatcctgagg atcctcaaga aggagcagat ggtccaggca cgcatcatcg tcataacggg
     3181 ttacggcagt atcccgcttg cagtcgaggc gatgaggctc ggcgccgtca actttctcac
     3241 caagcccttc ggggccgcgg agctgagagg cgccctgaac gagtgcttcg cagaggccgc
     3301 cgacgagtcg tcggaagact cgctcgggat gctcatcggc tcgagcgact acatgaggga
     3361 actcaaagag acgatcaggc gtgtctcgaa gaccgactcc tcggtgctca tcacgggcga
     3421 gagcgggacc ggcaaggaac ttgtcgccag gaccatacac aacctcagca ggcgctcggg
     3481 gatgccgttc gtggcagtcg actgttcctc cctcgtgccg aacctcatgg agagcgagct
     3541 cttcggccac gtcaagggcg cgttcagcgg cgcgtacgaa agccgccagg ggcgtttcca
     3601 ggtggcgcac agagggaccc tcttcctcga cgagatctcg aacataagcc tcgacatcca
     3661 ggcgaagctc ctgcgtgtcg tccaggagca ggaggtgccc agggtgggca gctcgaaacc
     3721 cgagaaggtg gacgtgcgcc tgatcacggc cacgaaccgg gacctcaggg ccgagggcga
     3781 gtcagggagg ttccgcgaag acctcttcta ccggatctct gtcgtgccca tgcacataag
     3841 gcctctcagg gagcaccgca ccgacatcat ggacatcgcc cgccactacc tcgagatcta
     3901 ccgggagaga cacggtgcaa aggtcaggag gttctcgatc gaggccatcc agtcgctcaa
     3961 cagctactcc tggccgggta acgtgaggga gctgaagaac accgtggaac gcctctgcgt
     4021 cctgtgcgac cacgaggagg tgacgctgtc cgacatcatg tactacggcc aggacccgag
     4081 gggcaaggcg cccgtggtgg acccgttctc ggggaggatg aggctcgtcg acgtggaacg
     4141 ggaccacatc gagaaggccc tcagacactt caacttccac atgaacaaga cggcgcagtt
     4201 cctgggcata gaccgcaaga cactgcgctt aaagatccgg acctacggca tcaagacccc
     4261 gggcaacaac gaaaaggagg aatgacgttc gcgccgtgag cccttcaaca ggtgcgtccg
     4321 tcgcccgggt gccgcagcct cgagggtcag ctcgtacgag acgcgacgct cgggatgtcg
     4381 ccctcgaggt gaagccacgg gtgcgacgaa tgccgtgctt caaggttgac ggacaaccag
     4441 atgtgaacct tgggcggcgg cctcgggcat cacccgcctc ggtgacaaca cgaagagccg
     4501 ctcgccaggg tccctgtcgc cgtgcctcat accacccacg gggacaaccc atcgcccagg
     4561 ggtcaacttg ctccgacatg gggagtttca ccccattccc ccgggttctc gggatcccgc
     4621 tttcgcccta tgagcaatat atttcaacca gttacacatt ggcaccctct ttgctgtgtc
     4681 atcagtacga tatccagttt cgaggaggct ttatggaatc caaaaagaag attctcttga
     4741 tcgacgacga caaggacttc ctcatggcca ccaagctcat tctcgagaag gggggatacg
     4801 aggtgttcct cgccgaggac ggcaagagcg ggctggagat ggcccgcaag gtatcgcccg
     4861 acctcgcgat catcgacatg atgatggaga cctggagcga ggggttcaac gtcgtctccc
     4921 gtctcaggac atccgagacc ggcaagcaga tccccctcat catgctcagc gccgtggatc
     4981 tccaggggcc gtaccagtcc ttcgagcctc ccgagaatct ccccaccgtg gacctcgtcc
     5041 agcacaagcc catcaaggcg gacgagctgc tcgggcacat ctccaccctc ctgaagaagc
     5101 agtgaccatg ggggactcat cgggggggcc tgccagcatc cttatcatcg acgacgagga
     5161 gaccatcctg gacgggtgca ggcaggcgct cgagaaatca gggtacacgg tgatcacgtc
     5221 ctccgacggt cacg
//
LOCUS       DAOSCR010000120         5232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_9973_length_5232_cov_2.832528, whole genome shotgun sequence.
ACCESSION   DAOSCR010000120 DAOSCR010000000
VERSION     DAOSCR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9973_length_5232_cov_2.832528"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..575)
                     /locus_tag="PLT69_04310"
     CDS             complement(168..575)
                     /locus_tag="PLT69_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13697.1"
                     /translation="MKRILLVEDDEHLQFLIKEELVEEGYEVVVASDGVEAMSMLLSD
                     RSPRPDLIILDIRMPRMDGFETMGHILKSRVDSPVILHSAFSGYRNHPLSLAADEYVV
                     KSHDFTRLKDAVSRLLKAGKTSVRDFGEGVQAA"
     gene            980..1942
                     /locus_tag="PLT69_04315"
     CDS             980..1942
                     /locus_tag="PLT69_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ13698.1"
                     /translation="MGLVSPLTMRQADFEAAGIACTEVTERIFVIHGNNRSRTPFSHS
                     FLVLDTVNVLFDAGCGLDVIERLLGLVRIDRLFLSHSHVDHTAGCRLMNEKTDCEIVV
                     PETSSDTIANAELLARRFVGDDLVPLWMETYPVLTGFRDFSFHSTYDHAYELRTGHLT
                     FLAVPTPGHLEDHTCFFEPDRRILIGFDIDLSPFGPWYGNPESDIDAFRASISRIREL
                     KPEIYLPSHAKPVRSAHFEKRLARYEASFDERDRLILAMLTETPGMDLGAIVRRSPFY
                     DADHSTITDELLWFGEEQMVQKHLDRLLLAGVIRCDRGRYLVSP"
     gene            complement(2236..3327)
                     /gene="rodA"
                     /locus_tag="PLT69_04320"
     CDS             complement(2236..3327)
                     /gene="rodA"
                     /locus_tag="PLT69_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOJ13699.1"
                     /translation="MIRYERIAQHLDWGVVTTTCALVVIGLMSVYSSTSASGPSIFLR
                     QLTWVCMGCVFLVAAFAVDYRNLMYSAWPFYALVLLGLVLVFVIGREISGARRWIELG
                     PMRMQPSEIVKIFVIVWLAYWSEKNRHIVAYRFRDLLFPVSVVALPVILILLQPDLGT
                     AGIVSFIACAMFIMMGIRRSTLLALSGMVLAGLPAAWFALKDYQKTRILTFLDPSRDP
                     QGSGYHAIQSKIAVGSGGLLGKGFLHGTQTQLNFLPEHHTDFIFSVVAEEWGFAGSIL
                     LITLYLILITKIIDIGLKAKDRFGALICFGIASYFTFHVFINISMAVGIFPVVGVPLP
                     FVSYGGTFMLINLVCIGLVLNVAWRRFIF"
     gene            complement(3293..5119)
                     /gene="mrdA"
                     /locus_tag="PLT69_04325"
     CDS             complement(3293..5119)
                     /gene="mrdA"
                     /locus_tag="PLT69_04325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ13700.1"
                     /translation="MEPNIANPHLEEDLKKRSRYLLGFVIVLLGILALRLLYMQIIRG
                     QYYEELSKNNRIRILPVLAPRGRILDRNGVVLADNRPALNVVVLPEDVTDVARTARRL
                     APLVDKDAKAIEQAVIKGKTRPYDPVAVARDVDFEQVAKVEMEMFRLPGVSIEAVPER
                     EYLFGDLACHVIGYIGEVSKARLEEMRGMDYSPGDLVGKNGIEAYCEAALRGTKGRRV
                     VEVDATGRLSSVLEEVAPVPGRDVRLTLDADLHAVARHSLGDRAGAVVAMVPDTGEVL
                     VMESTPGYEPSIFAGTLSPTQWREIMNDPLHPLENRALRGAYPPGSVYKVVVSLAAFK
                     AGILNPDARVYCPGFYSMGRVSFGCWRREGHGYVDFVRALTESCDVYFYNLGRALGVD
                     AIAEVATQLGLGRKTGVLYNEASGVIPTRAWKRKRFGQPWHPGESVIHAIGQGFTAVT
                     PIQVAKAMSAVLNGGYVLKPRIVADEPRIVERDLAIPRMQVEIIKRGLWGVVSDPRGT
                     AHSIWDKDVPMGGKTGTAQVARGYTSKLPDEADIPYRYRDHAWFFGFSPIERPEIVVV
                     AMVEHGGHGGAVAAPIVRDVIKGYYLIKGSRHDQVRADSPTP"
BASE COUNT          992 a         1779 c         1561 g          900 t
ORIGIN      
        1 gacagaggaa gggcagccat gcggatggcg aggcgacttc ccgggggagg cgggactcct
       61 cccgctcagg ccgccgggcg cgagtcttca ggcggtctgg cgacataccg gcccctccgg
      121 gcggttgccg tcttttgccg tgtccagctc gaggggcaaa gagcggctca cgccgcctgc
      181 accccctccc cgaagtcacg caccgaggtc ttccccgcct tgaggagccg gctcacggcg
      241 tccttgaggc gcgtgaagtc gtgggacttc acgacgtatt cgtcggctgc gagcgaaaga
      301 gggtgattcc ggtaccccga gaacgccgag tggagtatca cgggggagtc cacccgcgac
      361 ttgagtatgt gccccatggt ctcgaacccg tccatcctcg gcatgcgtat gtccaggatg
      421 atgaggtccg gcctgggcga gcgatcggag aggagcatgg acatcgcctc cacgccgtcc
      481 gaggccacca ccacctcgta cccctcctcg accagttcct ccttgatgag gaactggagg
      541 tgctcatcgt cttccaccag caggatccgc ttcatgccca cctccattca acatgatccg
      601 cctgtagccc ggcaatcgtg tcacccagca aaagcatcca gccggcaccc gacagaccgc
      661 atttatgcat gaaaaatgcc aagaataccc cgtaagaaca ggcgcctcga cgaccgggga
      721 ccggggcacg gcccttgacc gatgccgaga ccttccctct cacgccgcac caggagacaa
      781 accggcagaa atgcgtcacc cccgggagag gcaatgtcaa gatattgacg gaaccgtgcc
      841 gcgctccggc cgacacgcgg cctctgtctg ccaggtggtc acggcctcat ggatgaggct
      901 ttctttctcg cctgtttcaa ggccgcctgc ggaaaccgtc gcgcgccgcg ccgcatacga
      961 gccttgaccg gcgggcgcat tgggtctagt ctccccattg accatgcggc aggcggattt
     1021 cgaagcggcg ggcatcgcgt gcaccgaggt gacggagagg atcttcgtca tccacggaaa
     1081 caaccgttcc cgtacaccct tttcccattc cttcctggtg ctggacaccg tgaacgtgct
     1141 cttcgacgcg gggtgcggcc tcgacgtcat cgaaaggctg ctgggtctcg tgcgtatcga
     1201 ccgcctgttc ctgtcccatt cccatgtgga ccataccgcc ggctgtcgcc tcatgaacga
     1261 aaagacggac tgcgagatcg tggtccccga aacgtcgagc gacaccatcg cgaacgcgga
     1321 gctgctcgcg aggcgtttcg tgggcgatga cctcgttccc ctgtggatgg agacctaccc
     1381 ggtactcacc gggttcaggg acttctcctt ccactccacc tacgaccacg catacgaact
     1441 cagaaccggg cacctgacct tcctcgcggt ccccacgccc gggcacctcg aggaccacac
     1501 ctgcttcttt gaaccggaca ggcgcatcct catcgggttc gacatagacc tgagcccctt
     1561 cggcccctgg tacgggaacc ccgagagcga catagacgcg ttcagagcct ccatctcgcg
     1621 catcagggag ttgaagccgg agatctacct cccgtcccac gcaaaaccgg tgaggtcggc
     1681 gcacttcgag aagcgtctcg cacgctacga ggcctccttc gacgagcggg acaggctcat
     1741 cctcgcgatg ctcaccgaga ccccggggat ggacctgggg gcgatcgtga gacgctcgcc
     1801 cttctacgac gcggaccact cgaccatcac ggacgagctc ctctggttcg gagaggaaca
     1861 gatggtgcaa aaacacctcg atcgcctcct tctcgcgggt gtcatcaggt gcgacagggg
     1921 ccgatacctg gtctccccgt agggggagaa acgtccgccc caagctcaga ccatggaggg
     1981 cgaggaggcc gtctccctcc cccgtcaacc aaccggcccg ccttcgtagc caagccatct
     2041 gcggccgggc ggcctccccg ccgtccccat cgcgggggct gatgccgacc ttgagcccat
     2101 ggatttcggt catcaagggc cccgcccacc ctcgtgctcc aggaaaggcg ccgtcatgga
     2161 aggatgccca acgaacaaag ccgtcctttc atcgccgcac ggtcttgggt cgggccggcg
     2221 gccttttgcc cgggctcaga agatgaacct cctccacgcc acgttcagca ccaggcctat
     2281 gcacacgaga ttgatgagca tgaacgtgcc cccgtaactc acgaagggaa gaggcacccc
     2341 caccaccggg aagatgccca ccgccatgga gatattgatg aacacgtgga aggtgaaata
     2401 ggaggctatg ccgaaacaga tcaaggcgcc gaagcggtct ttcgccttca ggcctatgtc
     2461 gatgatcttc gtgatgagga tgaggtagag ggtgatgagg aggatcgacc cagcgaaacc
     2521 ccactcctcc gccaccacgg agaagatgaa atcggtatgg tgctcgggca ggaagttgag
     2581 ctgcgtctgt gtcccgtgca ggaagccctt gcccaacaga ccgcccgagc ccaccgcgat
     2641 cttggactgt atggcgtggt aaccgctgcc ctgcggatcc cgcgaggggt ccaggaaagt
     2701 gaggatcctc gtcttctggt agtccttgag cgcgaaccat gccgccggca accccgcgag
     2761 gaccatgccg gagagtgcga gcagggtcga gcgccgtatg cccatcatga tgaacatggc
     2821 gcacgcgatg aaggaaacga tgccggcagt tcccaaatcc ggctgcaaca ggatgaggat
     2881 gaccggcagg gccacgacgg aaacggggaa caggagatcc cgaaacctgt aggcgacgat
     2941 gtgcctgttc ttctccgacc agtacgcgag ccacacgatg acgaagatct tcacgatctc
     3001 cgaaggctgc atgcgcatgg gcccgagctc tatccaccgc ctggccccgc tgatctccct
     3061 tccgatcacg aacacgagca cgaggccgag gagcacgagg gcgtagaaag gccaggccga
     3121 gtacatgagg ttcctgtagt cgacggcgaa ggcggcgacg aggaacacgc accccatgca
     3181 cacccacgtg agctggcgga ggaagatgga cgggccggac gccgacgtag agctgtagac
     3241 actcatgagc ccgatgacca ccagcgcgca ggtggtcgtg acgacgcccc agtcaaggtg
     3301 ttgggctatc cgctcgtacc tgatcatgac gggatccctt gatgaggtaa tatcccttta
     3361 tgacatccct gacaatgggt gcggccacgg ccccaccgtg cccgccgtgt tcgaccatcg
     3421 ccaccaccac gatctcgggc cgctcgatgg gcgagaaccc gaagaaccac gcatggtcac
     3481 gatacctgta cgggatgtcc gcctcgtctg gaagtttcga ggtgtacccc ctggccacct
     3541 gggcggtacc cgtctttccc cccatgggca cgtccttgtc ccagatggaa tgcgccgtac
     3601 cgcgggggtc cgagaccaca ccccaaaggc ccctcttgat gatctcgacc tgcatcctcg
     3661 ggatggcgag gtcgcgttcc acgatcctgg gctcgtccgc gacgatcctc ggcttgagca
     3721 cgtacccgcc gttgaggacg gcgctcatgg ccttggccac ctggatgggg gtcaccgccg
     3781 tgaatccttg gccgatcgca tggatcacgc tctcccccgg gtgccacggc tgtccgaaac
     3841 gcttcctctt ccaggccctc gtaggtatca cgcccgaggc ctcgttatag agcacgcccg
     3901 tcttcctgcc gagcccgagc tgtgtggcga cctcggcgat ggcatcgact cccaacgccc
     3961 tcccgaggtt gtagaaatac acgtcgcacg actctgtcag ggccctgacg aaatcaacgt
     4021 acccgtgtcc ctccctcctc cagcacccga acgagaccct ccccatggag tagaaacccg
     4081 ggcagtacac cctggcatcc gggttcaaga tcccagcctt gaacgcggcc aggctcacca
     4141 cgaccttgta caccgaacca ggcgggtagg cgcccctgag cgctcggttc tccagggggt
     4201 gcaaggggtc gttcatgatc tccctccact gtgtggggct cagcgtcccc gcgaagatgg
     4261 agggttcgta gccgggggtg ctctccatga cgagcacctc gcccgtatcg gggaccatgg
     4321 ccaccaccgc tcccgccctg tcgccgaggc tgtgccgcgc cacggcatgg agatcggcgt
     4381 ccagcgtgag cctcacgtcc cggccgggca cgggggcgac ctcctcgagc acgctggaga
     4441 gccttcccgt ggcatccacc tccacgacgc gcctgccctt ggtgccacgg agcgcagcct
     4501 cgcagtaggc ctcgatcccg ttcttcccca cgaggtcacc cggcgagtag tccatgccgc
     4561 gcatctcctc gagcctggcc ttcgagacct ccccgatgta cccgatcacg tggcaggcga
     4621 gatccccgaa gagatactcc ctctcgggca ccgcctctat ggaaacgccg ggcagcctga
     4681 acatctccat ctccaccttc gccacctgtt cgaagtcgac gtcccttgcc acggcgacgg
     4741 ggtcgtacgg cctggtcttg cccttgatca ccgcctgctc gatggcctta gcgtccttgt
     4801 ccacgagggg tgccagacgc cgggccgtcc tggcgacgtc cgtgacatcc tcgggcagca
     4861 ccaccacgtt cagggcgggc ctgttgtccg cgaggaccac cccgttgcgg tcgaggatcc
     4921 tcccgcgggg ggcgaggacg ggcaggatcc tgatcctgtt gttcttcgag agctcttcgt
     4981 agtactggcc gcggatgatc tgcatgtaca agagccgcag ggccaggatc ccgagcagca
     5041 cgatgacgaa ccccagcagg tagcgggacc tcttcttgag atcctcttcg aggtgcgggt
     5101 tggcgatgtt aggttccata gaccttcttg agccttccca tgaaccctac catgacgggt
     5161 cccgcacacc cggtcaggag acacccggca agcatggaca cgacctcgaa gccctgcggg
     5221 acgacgccct gg
//
LOCUS       DAOSCR010000121         5178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10085_length_5178_cov_3.220964, whole genome shotgun sequence.
ACCESSION   DAOSCR010000121 DAOSCR010000000
VERSION     DAOSCR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10085_length_5178_cov_3.220964"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLT69_04330"
     CDS             <1..889
                     /locus_tag="PLT69_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ13701.1"
                     /translation="QAAMHVDTATREVASGAQGLSQATQEQASAIEEVAATIEQMTSS
                     IKHNAENASDGRTKATEMVRMANASGEAAQELVRGMSEISEASKKIGDIIVTVNEVAF
                     QTNLLALNAAVEAARAGEHGKGFAVVAEEVRALAQRSAEAAREIKALIEDTVNKIAAG
                     DTMVKKSGESLEQIIRHIQDLSQVMEEIAAASSEQASGVDELNRAISQIDTTTQQNAS
                     TVEELASTSDNLSMEAKELAEVVARFRVSREIESSSAKKGPYRAKAPRALTQKPVKKA
                     ATREPEPPEADFEDDFEEF"
     gene            905..1387
                     /locus_tag="PLT69_04335"
     CDS             905..1387
                     /locus_tag="PLT69_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ13702.1"
                     /translation="MARAVNTETAKNLSNQFVTFQLGSETYGIPILKLNEIIAYQNCT
                     QIPNVPKFIKGVLNLRGVVVPVFDLRERFGMETKEYDQFTVIMVLDVSGRTMGLVVDA
                     VSDVVTLHDDDIKPRPHFSTGISTEFIKGMGIKDGSFIIILDTDRLLSDEELNMVDGV
                     "
     gene            1384..2229
                     /locus_tag="PLT69_04340"
     CDS             1384..2229
                     /locus_tag="PLT69_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HOJ13703.1"
                     /translation="MTPDLCTRTYTITDREFELFRDLIFRQTGIHLSDRKKNLVVARL
                     SKRLRALDLENFTEYYRFLTERPDASSELVNLVNRVTTNKTDFFRENHHFDFLRGDVL
                     PEILENASRGGPKKLRIWSAGCSTGEEPYSIAMTVHDFFSNERGWDIKILATDLDTEV
                     LGFAYRGVYPSQRVAPVPGPFLTKYFERTAEGYSVKRQIRDMVIFRRLDLMDAMFPLK
                     SQVDVIFCRNVMIYFNDETKASLVGKFHRHLRNDGYLFIGHSESLMHMKDLFTFVRHT
                     VYRKV"
     gene            2232..2828
                     /locus_tag="PLT69_04345"
     CDS             2232..2828
                     /locus_tag="PLT69_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HOJ13704.1"
                     /translation="MYRRYSDKFKRPMVIIYPGEFHVSSKEIVATVLGSCISVCIRDP
                     EAGIGGMNHFMLPGDVRSEEMFLSESTKYGMFAMEQLIKAITDKGGKQRNFQAKVFGG
                     GHVLNFRKSDGNVPESNIEFVRAFLKMEGIPIVSEDVGGYAGRKILYFPDTGRVLLKR
                     LKSTIDSRLIEAEEAYKIRLFAQKDHLGTEGEKEPGKE"
     gene            2836..3924
                     /locus_tag="PLT69_04350"
     CDS             2836..3924
                     /locus_tag="PLT69_04350"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOJ13705.1"
                     /translation="MERVRVLIVDDSALVRKVLKEILESDPGIEVAGTAPDPLIAVRK
                     IKELKPDVLTLDIEMPKMDGLTFLSRLMRSTPMPVVMFSSLTEAGANATMKALSLGAI
                     DFLAKPKVKLSEGIEDLRYELCTKVRTAAHAKIKKAISPPNLSVPPKLSVDAVIKPER
                     SPRIKEGAKVLVIGASTGGTVALEQILSKMPADGPATLIVQHMPPVFTSSFAKRVDSL
                     SAMKVIEARDNDELRQGQALIAPGGKHMIVHKGQRGYFVRVMDGPPVNRHIPSVDVLF
                     RSAANVLGPNAVGVILTGMGDDGARGLKEMRDAGAFTIAQDEESCVVFGMPKVAIEMG
                     GACRVLPLSGIASFVMDMLSPEGTNDRT"
     gene            3945..>5178
                     /locus_tag="PLT69_04355"
     CDS             3945..>5178
                     /locus_tag="PLT69_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13706.1"
                     /translation="MGEIRNILIIDPDKRSGDLSRKLRRKGFQAAWAKNVEDALSLVM
                     DGKPDLVLFGLDGIPERSLAAIGEIGELSPGTVVVAAAASAAEAGTAVECMRRGTMDL
                     LFKPVTPGALLECIRRIERRLNNLCIGMTPNTGCVAREHKTLVFPNEIENLPYLINQA
                     VLNAPVVCRDVETLKMALSEILINAIEHGNLEITMEEKASAVKKGAFEKLVHRRLADP
                     RLSSRRVTLEVFMDEDRLEYVVTDEGRGFDYRKEVDPDPNAHIGSGLGIQIARAFFHE
                     VRYEGEGNRVHLVYYRDPRLHRGGRETGPASLTMNAHRPVGGKSGAAGAGDLGDGVFL
                     TGTMNIDVIVQLAESFPMGLLLVTDSERIAMWNQKAEQITSITSSSLVGAHVSEAPAF
                     VRHLLDQSSSHADLVLDEF"
BASE COUNT         1126 a         1570 c         1661 g          821 t
ORIGIN      
        1 gcaggcggcc atgcacgtgg acaccgcgac cagggaggtg gcatccggcg cgcaagggct
       61 ctcccaggcg acccaggagc aggcctcggc catcgaggag gtggcggcca ccatcgagca
      121 gatgacttcc tcgattaagc acaacgccga gaacgcgagc gacggcagga cgaaggccac
      181 ggagatggta cgcatggcga acgcgagcgg tgaggcggcg caggagctcg tgaggggcat
      241 gagcgagatc tcggaggcat cgaagaagat cggcgacatc atcgtcacgg tcaacgaggt
      301 cgcgttccag acgaacctcc tcgccctcaa cgccgctgtg gaggcggcca gagccgggga
      361 acacggcaag gggttcgccg tggtcgccga ggaggtgcgg gcgctcgcac agaggtcggc
      421 cgaggccgcg cgcgagatca aggccctcat cgaggacacg gtgaacaaga tcgcggccgg
      481 cgacaccatg gtcaagaagt ccggcgagtc cctggagcag atcatcaggc acatccagga
      541 cctctcccag gtcatggagg agatcgcggc cgcatccagc gaacaggcca gcggcgtcga
      601 cgagctcaac agggcgatct cccagatcga cacgacgaca cagcagaacg cctccacggt
      661 ggaggaactg gcgagcacct cggacaacct cagcatggag gccaaggaac tcgccgaggt
      721 cgtggccagg ttcagggtgt cgcgggagat cgagtcctcc tcggccaaga aggggccgta
      781 ccgcgccaag gcaccccgag ccctcacgca gaaaccggtc aagaaggcgg cgaccaggga
      841 gcccgagcct ccagaggccg acttcgagga cgacttcgag gaattctgat acggaggcct
      901 gctgatggcg agagcggtca acacagagac ggccaagaac ctgtccaacc agttcgtgac
      961 cttccagctt ggatcggaga cctacgggat ccccatcctc aagctcaacg agatcatcgc
     1021 ctaccagaac tgcacccaga tccccaacgt gccgaagttc atcaaggggg tgctcaacct
     1081 gagaggggtg gtggtccctg tcttcgacct cagggaacgg ttcggcatgg agaccaagga
     1141 gtacgaccag ttcacggtca tcatggtgct cgacgtatcg ggcaggacca tggggctcgt
     1201 ggtggacgcg gtgagtgacg tcgtcaccct gcacgacgac gacatcaagc cgcgcccgca
     1261 cttctcgacg gggatcagca ccgagttcat caagggcatg gggatcaagg acggatcatt
     1321 catcatcata ctggacaccg acaggttgct ctccgacgag gagttgaaca tggtcgacgg
     1381 ggtatgacgc ctgacctgtg cacacggaca tacaccatca cggacaggga attcgagctc
     1441 ttccgggacc tcatcttccg gcagaccggg atccatctct cggaccgcaa gaagaacctg
     1501 gtcgtggccc gtctctcgaa gcgcctcagg gcgctcgacc tggagaactt caccgagtac
     1561 taccggtttc tcacagaacg gccggacgca tcgagcgaac tggtcaacct ggtcaacagg
     1621 gtgaccacga acaagactga cttcttcagg gagaaccacc atttcgactt cctgaggggc
     1681 gatgtccttc ccgagatcct cgagaacgcc agcagggggg gaccgaaaaa actgaggatc
     1741 tggtccgccg ggtgctcgac aggcgaggaa ccctacagca tcgccatgac ggtgcacgat
     1801 ttcttctcga acgagcgggg ttgggacatc aagatcctcg ccaccgacct ggacaccgag
     1861 gtgctcggtt tcgcctaccg cggcgtgtac ccgagccaga gggttgcgcc cgtgcccggg
     1921 cctttcttga ccaaatactt cgagaggacc gccgaggggt actcggtcaa gaggcagatc
     1981 agggacatgg tgatcttcag gaggctggac ctgatggacg cgatgttccc cctcaagagc
     2041 caggtggacg tcatattctg caggaacgtc atgatctact tcaacgacga gacgaaggcc
     2101 tctctcgtgg ggaagttcca caggcacctg aggaacgacg ggtatctctt catcggccac
     2161 tccgagtcgc tcatgcatat gaaggacctg ttcacgttcg tcaggcacac ggtgtacagg
     2221 aaggtgtaga catgtacagg cgatacagcg acaagttcaa gcgccccatg gtgatcatct
     2281 accccggcga gttccacgtg agctccaagg agatcgtcgc cacggtgctc ggttcctgta
     2341 tttctgtgtg catcagggac cccgaggcgg gcattggcgg catgaaccac ttcatgctgc
     2401 ccggagatgt ccggtccgag gagatgttcc tatccgaatc caccaagtat gggatgttcg
     2461 ccatggagca gctcatcaag gcgatcacgg acaagggcgg gaagcagagg aacttccagg
     2521 cgaaggtctt cggcgggggg cacgtgctga acttcaggaa gtcggacggg aacgtgcccg
     2581 agtcgaacat cgagttcgtg agggccttct tgaagatgga gggcataccc atcgtgagcg
     2641 aggacgtggg cggctatgca ggcaggaaga tcctctactt cccggatacg ggaagggtcc
     2701 tgctcaagag actgaagtcg accatcgact ccaggctgat cgaggccgag gaggcataca
     2761 agatcaggct cttcgcgcag aaagaccacc tggggaccga gggcgagaaa gagccgggga
     2821 aagaatgagg ctgccatgga acgtgtaaga gttctcatcg tggacgattc ggccctcgta
     2881 aggaaggtcc tcaaggagat cctcgagtcg gaccccggca tagaggtggc ggggaccgcc
     2941 ccggaccccc tcatcgcggt gcgcaagatc aaggaactca agcccgacgt gctcaccctc
     3001 gacatcgaga tgcccaagat ggacgggctc acgttcctct cccgcctcat gcgatcgacg
     3061 cctatgccgg tggtcatgtt ctcttccctg accgaggcag gggccaacgc caccatgaag
     3121 gcgctgtccc tgggcgccat cgacttcctg gccaagccca aggtaaagct cagcgaaggc
     3181 atcgaggacc tgaggtacga gttgtgcacc aaggtccgta ccgcggccca cgcgaagatc
     3241 aagaaggcga tctctccacc caacctctcc gtccctccca agctgagcgt ggatgcggtg
     3301 atcaagcccg aacggtcgcc gaggatcaag gaaggcgcca aggtcttggt gataggggca
     3361 tccacgggtg ggaccgtcgc gctggagcag atcctcagca agatgccggc ggacggcccg
     3421 gccaccctca tcgtgcagca catgccaccc gtgttcacct cttccttcgc caagcgggtg
     3481 gactccctct cggcgatgaa ggtcatcgag gcccgcgaca acgacgagct caggcagggc
     3541 caggcgctga tagcgccggg cggcaaacac atgatcgtgc acaaggggca gaggggatac
     3601 ttcgtgaggg tgatggacgg cccgccggtc aacaggcaca tcccgtcggt ggacgttctc
     3661 ttcaggtccg ccgcgaacgt gctcgggccc aatgcggtcg gggtcatcct caccggcatg
     3721 ggtgacgacg gcgccagggg actcaaggaa atgagggacg cgggcgcctt caccatcgcg
     3781 caggacgagg agagctgcgt ggtgttcggc atgcccaagg ttgccatcga gatgggtggt
     3841 gcgtgcaggg tcctgcccct ctccggcatc gcgagcttcg tcatggacat gctatcgcct
     3901 gaaggaacga acgacaggac gtaacgccgc accgggacac gcacatgggg gagatcagga
     3961 acattctcat catcgaccct gacaagagat cgggtgatct ctccagaaaa ctcaggcgca
     4021 agggtttcca ggccgcgtgg gccaagaacg tcgaagatgc gctctccctc gtcatggatg
     4081 gcaagcccga cctcgtgctg ttcggtttgg acggcatacc cgagcgctcc cttgccgcca
     4141 taggcgagat cggcgagctc tcgcccggca ccgtggtggt ggcggcggca gcctcagcag
     4201 ccgaggccgg caccgccgtc gagtgcatgc ggagagggac gatggacctc ctcttcaagc
     4261 ccgtgacccc gggcgcgctc ctcgaatgca tacggcgcat agagagacga ctgaacaacc
     4321 tgtgcatcgg gatgacgccg aacaccgggt gcgtcgccag ggaacacaag accctcgtct
     4381 tccccaacga gatcgagaac ctcccctacc tgatcaacca ggcggtgctc aacgcccctg
     4441 tcgtgtgccg ggacgtcgag acgctgaaga tggcgctctc cgagatcctc atcaacgcca
     4501 tagagcacgg caacctcgag atcaccatgg aggagaaggc ctctgcggtc aagaaggggg
     4561 cgttcgagaa gctcgtgcac aggaggcttg ccgacccgag gctttcctcg aggagggtca
     4621 ccctcgaggt cttcatggac gaggacaggc tggaatacgt cgtgacggac gaggggagag
     4681 ggttcgacta ccgaaaggag gtcgaccccg accccaacgc ccacataggg agcgggctcg
     4741 ggatccagat agcgagagcc ttcttccacg aggtcaggta cgagggtgaa ggcaacaggg
     4801 tccatctcgt ctactaccgg gacccccgcc tgcatcgagg cggccgggaa acgggcccgg
     4861 cgtccttgac gatgaatgcg catcgacctg tgggcggaaa gagcggggcg gccggggcgg
     4921 gtgacctcgg cgacggggtg ttccttacgg gcaccatgaa catagacgtg atcgtccagc
     4981 ttgccgagtc cttccccatg gggctgctcc tcgtcaccga cagcgagcgc atagccatgt
     5041 ggaaccagaa ggccgagcag atcacctcca tcacgtcctc aagcctcgta ggcgcgcatg
     5101 tgtccgaggc ccccgccttt gtccgacacc tcctcgatca atcgagctcc catgccgacc
     5161 tcgtcctcga cgagttcg
//
LOCUS       DAOSCR010000122         5109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10240_length_5109_cov_4.037792, whole genome shotgun sequence.
ACCESSION   DAOSCR010000122 DAOSCR010000000
VERSION     DAOSCR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10240_length_5109_cov_4.037792"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..879)
                     /locus_tag="PLT69_04360"
     CDS             complement(34..879)
                     /locus_tag="PLT69_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ13707.1"
                     /translation="MKDFSGKTVYITGGSSGIGLETARLFASLGGDVVIFARDEERLA
                     SAARDIEGHRRSGSQKVHAVHMDVADPESVRTGIEEAIEKAGPPQVAIANAGVGYADL
                     FIDTPHEAFDAVMKTNVYGVRSFVANVLPHMVGAKGTIVIVSSAAGLMGMYGYTTYGT
                     SKYALVGFAECLRAELRTMGMTVTLVCPGEVDTPFVPKEAERLPFESRVVKSFCGRIS
                     PSRVAHAIVKGVAGKRFLVIPGVLTNLMFALHRFSNGMLTRYTSDLVVFVLRWYVSKQ
                     AARRR"
     gene            complement(1036..2001)
                     /locus_tag="PLT69_04365"
     CDS             complement(1036..2001)
                     /locus_tag="PLT69_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOJ13708.1"
                     /translation="MAGYTPKAHEDTGTLGEALILEIKPNSLDDGRGIRTVVFFKGCP
                     LDCVWCHNPESKSTRAELSYDAKACIGCNTCLEVCPAQAIDPGSPSFVDRARCTLCFA
                     CTVECPTGALERVGIPMGVEDVLARILPDKPFFVTSGGGVTLSGGEPTLAMDFSARLA
                     SRLKAEGIHTLLETCGLFDLKTFTHTLMPFVDEIFYDIKIMDAGLHRRFCGADNRTIL
                     SNFAAVLNMSRNGGPGVLPRIPLVPGITDTDENMNAIGKFLEGLGCRRAAVLAYNPLW
                     PEKCLKLGLKVPFEFEKEHKERTWYPRQRLSRAIEILSGYGVEAG"
     gene            complement(2041..5037)
                     /locus_tag="PLT69_04370"
     CDS             complement(2041..5037)
                     /locus_tag="PLT69_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ13709.1"
                     /translation="MGLAEPLSDRSVFRPTQIMVTTLLRLMAAAYTWRGSLNRYLASA
                     TGWIDFTVGIRTESGSVTAGISFRQGRARVEDRVPEGADVILTFTSDRAVLKLLNATP
                     TEQIFMLLKSELFYEGSAAYLNCFFFLLSVLIHKKQKKLMLKERTTRSRQLLKESPRP
                     RTDLSEGIKARAKNRLAAESRDPGVKYLDEPYLSSYGIEDFPRLQRFLDEHFTTKAEV
                     CPELPKLVTEWHLENGFETDRQGKPWNPIMRKALAFRHLMERKKPIIHKDDLIAGTTT
                     TMPIGVVVYPEGHGTMIWSELLTVGDRTLNPYDISEDTRRLLHHTVFPYWAHRNFREW
                     VRDRYGKPLCQRIDERFAVYFDWKQATISHTIPDFPKILRLGTKGMIDEIRAHLQDHP
                     HDEEERVTLEAMIIALEGLQAYSRNLAKQASIDAAREPDPARRAELEHLAKVCSQVVE
                     HPARTLDEAVNAVWITWVALHQESTNAGLSLGRLDQWLQPYFEADMAKLASEEERRAY
                     IKHAIELVGCFYMRCTDHLPLTPDLANFYFGGSSSDQAITLGGVTPEGEDAVCDMTYI
                     FLKVTEMLSIRDPNVNARYHPRINSDTYLKRLCEVNLVTAATPSMHNDEVIFTSLSQF
                     GYDIRDIRNWSATGCVEPTLSGRHIGHTNMQMMNMVAALEMALNNGYHPVMKWKLGPE
                     TGSVEKGDFKTFDDFFDAFTTQFKFLIDQSIEYNHMLAEAHQELRPTPLLSSLIDGCV
                     DKGRDVTKGGAVYNSSGTAIIGLADVTDSLMVIKKLVYDEGKVSFERLKKAIDANFEN
                     DPALYAMVTRKVPLFGSGSEEAVAMANRIAKWAHDYYQGLDHYRGGKYTVGFWSMSNH
                     VIFGTLTGALPSGRLAGKPFTPGLTPQPIASKSLLDNIRDVAKLDPMNITNNMAFNVK
                     VVPSAKDTREKTIGDMFSYVKTYFDMGGMQMQMNVVTSETLRDAMAHPENYRNLLVRI
                     SGYNAYFVTLNKDMQLELIERAEYGI"
BASE COUNT          821 a         1597 c         1671 g         1020 t
ORIGIN      
        1 gcctgagggt gaacgatcgc ttccctggcg atcctacctg cgccttgcgg cctgtttcga
       61 cacgtaccac ctgagcacga agaccacgag gtccgaggtg taccgcgtga gcatgccgtt
      121 cgagaaccgg tgcagcgcga acatgaggtt cgtgagcacg ccggggatga ccaggaagcg
      181 cttcccggcc acccccttga cgatcgcgtg agccaccctc gacggggaga tgcggccgca
      241 gaagctcttc accacgcgcg attcgaaggg caggcgttcg gcctccttcg ggacgaaggg
      301 cgtgtccact tcgccggggc acacgagggt gacggtcatg cccattgttc tcagttcggc
      361 cctcaggcac tcggcgaacc cgacaagcgc gtacttcgac gtgccgtagg tggtgtagcc
      421 gtacatgccc atgagcccgg cggcggagga gacaatcacg atggtcccct tcgccccgac
      481 catgtggggg agcacgttcg cgacgaagga gcgcacgccg tagacgttcg tcttcatgac
      541 cgcgtcgaag gcctcatgcg gggtgtcgat gaagaggtcc gcatacccga cgcccgcgtt
      601 ggcgatcgcc acctgcggcg gccctgcctt ctcgatggct tcctcgatgc cggtccttac
      661 cgattccggg tccgccacgt ccatgtggac cgcgtgcact ttttggctgc ccgacctcct
      721 gtgaccctcg atgtccctgg cggccgaggc cagtctctcc tcgtccctgg cgaaaatcac
      781 gacgtcgccg cccagcgagg cgaacagccg cgccgtctcg agccctatgc cgctcgatcc
      841 acccgtgatg tacacggtct ttcctgaaaa gtccttcacc acaaacctcc tctttcagcc
      901 tgccgccctc tgcgccgggg ccccctcgag gtccggcgcc catctgtagg cccggaaccg
      961 tgcatccagg cctcgaggac agcccgcctc gatgccgcgc acaagcgcgc attcatctcg
     1021 ggcgccggcc aagcctcacc cggcctccac gccatagccc gagaggatct cgatcgcgcg
     1081 ggagaggcgt tgtctcgggt accacgtcct ttccttgtgc tccttctcga actcgaaggg
     1141 gaccttgagc cccagcttca ggcacttctc gggccagagg gggttgtagg cgaggacggc
     1201 ggcgcgcctg cacccgagac cctcgaggaa cttcccgatg gcgttcatgt tctcgtccgt
     1261 gtccgtgatc ccggggacga gagggatcct cgggaggaca cccggtcccc cgtttctcga
     1321 catgttcagg acagcggcga agtttgacag gatcgtcctg ttgtccgcgc cgcagaagcg
     1381 acggtgcagg ccggcatcca tgatcttgat gtcgtagaag atctcgtcca cgaagggcat
     1441 gagcgtgtgc gtgaaggtct tgaggtcgaa caggccgcag gtctcgagca gggtgtggat
     1501 gccttcggcc ttgagcctgg aggcgagcct ggcggagaaa tccatggcga gggtcggttc
     1561 gccgcctgac agggtcaccc ctccgccgga ggtcacgaag aagggcttgt cagggaggat
     1621 cctggcgagc acgtcctcca cgcccatggg tatgcccacg cgttcgagcg cgcccgtggg
     1681 gcactcgacg gtgcaggcga agcacagggt gcacctggcg cggtcgacga aggaagggga
     1741 gccggggtcg atcgcctgtg caggacaaac ttccaggcag gtgttgcagc cgatgcacgc
     1801 cttcgcgtcg taggagagct cggcgcgcgt cgacttgctc tccgggttgt ggcaccacac
     1861 gcagtccagc gggcagccct tgaagaacac caccgtgcgg atgcctctcc cgtcgtccag
     1921 gctgttcggc ttgatctcca ggatgagcgc ctcgccgagc gtcccggtat cctcatgcgc
     1981 cttcggggtg tatccggcca cccatgtctc cttttcttcc gcgccgcctt gagaagcgtc
     2041 tcagatgccg tactctgcgc gctcgatgag ctcgagctgc atgtccttgt tgagggtgac
     2101 gaagtaggcg ttgtaacccg agatcctcac gaggaggttg cggtagttct cggggtgggc
     2161 catggcgtcc cggagggtct cggaggtcac cacgttcatc tgcatctgca tgccgcccat
     2221 gtcgaagtag gtcttcacgt aggagaacat gtccccgatg gtcttctcgc gggtgtcctt
     2281 ggccgacggc accaccttga cgttgaacgc catgttgttc gtgatgttca tggggtcgag
     2341 cttggccacg tccctgatgt tgtccaggag gctcttcgat gcgatgggct ggggggtgag
     2401 cccgggagtg aacggctttc ccgcgagcct gcccgacggc agggcgccgg tgagtgtgcc
     2461 gaagatcacg tggttggaca tggaccagaa cccgaccgtg tacttgccgc cccggtagtg
     2521 atcgaggccc tggtagtagt cgtgggccca cttggcgatg cggttggcca tggcaacggc
     2581 ctcctcgctc cccgagccga acagtgggac ctttctcgtc accatcgcgt agagcgccgg
     2641 gtcgttctcg aagttcgcgt cgatcgcctt cttgagcctc tcgaacgaga ccttcccctc
     2701 gtcgtacacg agcttcttga tcaccatgag cgagtcggtc acgtcggcga gcccgatgat
     2761 cgccgtgccg gagctgttgt agacggcgcc gcccttggtg acgtcccgcc ccttgtcgac
     2821 gcagccgtct atcagggaag agagcagggg ggtcggcctc agctcctggt gagcctctgc
     2881 gagcatgtgg ttgtactcga tggactggtc gatgaggaac ttgaactggg tcgtgaaggc
     2941 gtcgaagaag tcgtcgaagg tcttgaagtc ccccttctcg acgctgcccg tctcaggccc
     3001 cagcttccac ttcatgacag ggtggtaccc gttgttgagg gccatctcga gggccgcgac
     3061 catgttcatc atctgcatgt tcgtgtgccc gatgtgcctc cccgagagcg tcggctccac
     3121 gcagccggtc gccgaccagt tcctgatgtc cctgatgtcg tacccgaact gcgagaggga
     3181 ggtgaagatc acctcgtcgt tgtgcatgga aggggtcgcc gcggtcacca ggttgacctc
     3241 gcacaggcgc ttcaggtacg tgtcgctgtt gatgcgcggg tggtacctgg cgttcacgtt
     3301 cgggtcgcgg atgctgagca tctccgtcac cttgaggaag atgtaggtca tgtcgcacac
     3361 cgcgtcctcg ccctcaggcg tcacgccgcc cagcgttatg gcctggtcgg acgagctccc
     3421 gccgaagtag aagttcgcca ggtcgggcgt gagcggcagg tggtcggtgc agcgcatgta
     3481 gaagcaaccg acgagctcga tggcgtgctt gatgtacgcg cggcgttcct cttcgctggc
     3541 gagcttggcc atgtctgcct cgaagtaggg ctgcagccat tggtcgagcc tccccaggga
     3601 cagcccggcg ttcgtgctct cctggtggag cgccacccag gtgatccaga cggcgttgac
     3661 cgcctcgtcc agggtgcgcg cagggtgttc caccacttgg gaacacacct tcgccaggtg
     3721 ctcgagctcg gccctgcggg cagggtccgg ctctcgagcc gcatcgatgg atgcctgctt
     3781 tgcgaggttg cgggagtatg cctgcagccc ctcgagcgcg atgatcatgg cctcgagggt
     3841 caccctctcc tcctcgtcgt ggggatggtc ttggaggtgg gcgcggatct cgtcgatcat
     3901 gccctttgtg ccgagcctca ggatcttcgg aaagtcgggg atggtgtgcg agatggtcgc
     3961 ctgcttccag tcgaaataca cggcgaaccg ctcgtctatc ctctggcaca gcggctttcc
     4021 gtagcggtcg cggacccact ccctgaagtt gcggtgggcc cagtagggaa agaccgtgtg
     4081 gtggaggagc cgtcgcgtgt cttcagagat gtcgtacggg ttgagggtgc ggtcgcccac
     4141 ggtgaggagc tcggaccaga tcatggtccc gtgaccctcg ggatagacca cgacgccgat
     4201 gggcatggtg gtggtcgtgc cggcgatgag gtcgtccttg tggatgatgg gtttcttccg
     4261 ctccatgagg tggcgaaacg cgagcgcctt gcgcatgatc gggttccagg gtttcccttg
     4321 ccggtctgtc tcgaaaccgt tctcgagatg ccactcggtg acgagcttgg ggagctcggg
     4381 gcagacttcg gccttcgtgg tgaagtgttc gtccaagaac ctctggaggc gagggaagtc
     4441 ctcgatgccg tatgaggaca ggtacggctc gtcgaggtat ttcacgcccg gatcccgcga
     4501 ttccgcagca aggcggttct tcgcccgcgc cttgatccct tcgctcaggt ctgtcctcgg
     4561 ccggggcgac tccttgagga gctgcctgga ccgggtggtc cgctccttga gcatgagttt
     4621 cttctgcttc ttgtggatga gcacggagag caggaagaag aagcagttga ggtacgccgc
     4681 gctcccctcg tagaagagct cgctcttgag cagcatgaag atctgttcgg tgggggtcgc
     4741 gttcaagagc ttcaagaccg ccctgtcgct cgtgaaggtg aggatgacgt ccgccccctc
     4801 gggcacgcgg tcttccacac gggccctgcc ttgcctgaag gatatcccgg ccgtcaccga
     4861 gccggactcg gtacggatcc cgaccgtgaa atctatccac cccgtggcgc ttgcaaggta
     4921 cctgttcagg gaccccctcc acgtgtaggc cgcggccatg agccgaagca gggtggtgac
     4981 catgatctgt gtcggcctga agaccgatcg atcggacaac ggttcagcaa gacccatcga
     5041 gcgcctcctt tcctcgaggc aaggggaccc acccctcttc gcgggcctca cgtgcgaagg
     5101 ggaagcccc
//
LOCUS       DAOSCR010000123         5057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10403_length_5057_cov_3.439624, whole genome shotgun sequence.
ACCESSION   DAOSCR010000123 DAOSCR010000000
VERSION     DAOSCR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10403_length_5057_cov_3.439624"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..379
                     /locus_tag="PLT69_04375"
     CDS             86..379
                     /locus_tag="PLT69_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ13710.1"
                     /translation="MAKPMEQITWQELETGAVVVEVGNSVQYETGSWRSERPVVDKEK
                     CIKCMFCWLYCPEGCIRQAEEGHFEADLTYCKGCGICAEECPVKCITMVIEEK"
     gene            383..1564
                     /locus_tag="PLT69_04380"
     CDS             383..1564
                     /locus_tag="PLT69_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ13711.1"
                     /translation="MAKTRKGVEVSIAIAEAVRQADVDVVAAYPITPQTHIVEHLSEL
                     VADGELDAEFVPVDSEHSAMSVCCGSAAVGARTFTSTSAQGLALMSEIYFIAAAMRLP
                     MVMALANRSLSSPLSIWNDHSDIMMVRDAGWICIFVENGQETYDNILCAFRMAENPEV
                     MLPVAVNLDGFILTHMIEPILYEDDDLVRSYLPWKPMKNALHPDRPVSMGCFGMPEIY
                     TEAQMNREMGVRNSYPHILDAWEEWGRLTGRHYRPVETYRCEDAEYCIVTMGSYGETA
                     MEVVDMLREEGEKVGVIKIRLWRPFPLEEFRKAALGRKCLIVMDRAITFGGNQGGPVA
                     TELRSALYGMDGAPSIVNYVAGLAGRDVSRKDFRNIILEGKAKAAKGEVDGFTLYGLR
                     G"
     gene            1572..2504
                     /gene="porB"
                     /locus_tag="PLT69_04385"
     CDS             1572..2504
                     /gene="porB"
                     /locus_tag="PLT69_04385"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HOJ13712.1"
                     /translation="MAKTSISSPKHVEREEFFAPGHRACQGCAEVLAVRLVLKALGKD
                     TIVAMATGCMEIISSPLPITSWRVPWIHNAFENAAAVISGCESGMKALMRKGKIPAKK
                     INFVGMGGDGATADIGMGPLSGALERGHDMIYVCYDNEAYMNTGIQRSSCTPYGASTT
                     TSPAGKKSIGQHTQKKNMVEIAAAHGIPYVATACPSFPFDLMEKVKKASETAGPAYIH
                     ILSVCPTGWRINPSEAIKYGRLAVNSCVFPLYEVDRGKYRLTYKPSDKKLIPVSEYLK
                     GQGRFRHLTDSDIETIQAATMKAYERLLRLSEQE"
     gene            2768..4237
                     /gene="ffh"
                     /locus_tag="PLT69_04390"
     CDS             2768..4237
                     /gene="ffh"
                     /locus_tag="PLT69_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOJ13713.1"
                     /translation="MLELITDGFRKAQDIIQGKVTIQEKHIEDALREIRLSLLEADVE
                     FHVVKGFIDHVKEKAIGEIVLTRVSHGGKKLKVTPEQHFVKICYDELVRLMGPVDTSL
                     ALGKRPVGAVMMVGLQGSGKTTTTAKMARYLQKKGKRPLMVAADIYRPAAIEQLKVLG
                     SRLDIPVFHAPGKTPPQICKDAMEAARFKGCDVVLFDTAGRTIVDDALMQELEEIKAS
                     TSPEDILLVVDAMIGQESVNVAKEFDRRLDLSGFVITKLDGDARGGAALSIKEVTGKP
                     IKFIGMGESLDRIEEFRPEGLASRILGYGDIVGLVKDFEEVIDEKKAEEDAEKLLRGE
                     FTFDDFLAQLKVMKKMGSLSDIISKLPLGQFGLPQGMKVDDRELVRVEAIIHSMTVYE
                     RNHPDCLDESRIQRIAKGSGQGRQRVVDLIARFRTMRQVMQGLGVGKKKVLGRLGIDP
                     GIAGALEKTVPKPQKKSLVDLKKRKEKRKSIKKSRKRNR"
     gene            4265..>5057
                     /locus_tag="PLT69_04395"
     CDS             4265..>5057
                     /locus_tag="PLT69_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ13714.1"
                     /translation="MAVSQDTNAELEVFRRISEAAVSLAPDVEAVARAILDIVVDVTP
                     AENASLMMPSADGKRLEIKAARGRNDRKGRYSERSLGQIFAIGEGIAGMAAETLTPVM
                     IHDTSADPRFQARSQSVRIGSLLSLPIVYNRDELVGVLNLSHPRAGVFEDGHLRLMSA
                     LLASAALAIRNAKLMREIDDMNRELKAELSLTDKAMSEFGRSILKVFNQMAIGILTTD
                     PTGAISAINKKAQEMLGKQAGETIADVVPVEIVHKVFTDKEGMTLD"
BASE COUNT         1104 a         1486 c         1676 g          791 t
ORIGIN      
        1 ggacaggttc gggtcgaaac tcggggaacg aaacctcaag gccctcgaga gggcctatca
       61 tgaagtcgag atcggggata gcaccatggc caagccgatg gagcagatca cgtggcagga
      121 actcgagacc ggcgccgtcg tcgtcgaggt cgggaactct gtccagtacg agacgggatc
      181 ctggcggtcc gagcggccgg tcgtcgacaa ggaaaagtgc atcaagtgca tgttctgctg
      241 gctgtactgt cccgaggggt gcatacgcca ggccgaggaa ggacacttcg aggccgatct
      301 cacgtactgc aaggggtgcg ggatctgcgc ggaggagtgc ccggtgaagt gcataacgat
      361 ggtcatcgag gagaagtgaa ccatggccaa gacacgaaag ggagtggagg tctcgatcgc
      421 gatagctgag gccgtcaggc aggcggacgt cgatgtggtc gcggcgtatc ccataacacc
      481 gcagacccac atcgtcgaac acctctccga gctcgtcgcc gacggcgaac tcgacgcgga
      541 gttcgtcccc gtggacagcg agcactcggc catgagcgtg tgctgtggtt cagcggcggt
      601 gggggcgagg acgttcacgt ccacgagtgc tcaggggctc gccctcatga gcgagatcta
      661 cttcatcgcc gccgccatga ggctccccat ggtcatggcg cttgcgaacc ggtcgttgtc
      721 gagcccgctg tccatctgga acgaccactc ggacatcatg atggtcaggg acgcgggctg
      781 gatctgcatc ttcgtggaaa acgggcagga gacgtacgac aacatcctgt gcgccttcag
      841 gatggccgag aaccccgagg tcatgttgcc cgtggcggtg aacctggacg ggttcatcct
      901 cacccacatg atcgagccca tactctacga agacgatgat ctcgtccgca gctacctgcc
      961 ctggaaaccc atgaagaacg cgctccatcc cgacaggccc gtttccatgg gctgcttcgg
     1021 catgcccgag atctacaccg aggcccagat gaaccgcgag atgggcgtga gaaactcgta
     1081 tccgcacatc ctcgacgcct gggaggagtg gggcaggctc accggcaggc actatcgccc
     1141 ggtggagaca taccggtgcg aggacgcgga atactgcatc gtcaccatgg gctcctacgg
     1201 cgagacggcc atggaggtgg tggacatgct cagggaagag ggcgagaagg tcggggtcat
     1261 caagatcagg ctctggagac cgttccccct cgaggagttc cgcaaggcag ctctcggcag
     1321 gaagtgcctg atcgtcatgg accgcgccat caccttcggg gggaaccagg gcggacccgt
     1381 ggccacggaa ctccgctcgg ccctttacgg catggacggc gccccctcca tcgtcaatta
     1441 cgtggcggga ctggcgggca gggatgtgtc gcgcaaggat ttcaggaaca tcatccttga
     1501 agggaaggcc aaggcggcca aaggcgaggt cgacggcttc accctatacg gtctgagggg
     1561 gtagtggtcc catggcaaag acaagcatct catctcccaa acacgtcgag agggaagagt
     1621 tcttcgcccc agggcacagg gcgtgccagg gatgcgcgga ggtcctggcc gtgcgcctcg
     1681 tgctcaaggc cctgggcaaa gacaccatcg tggccatggc cacgggctgc atggagatca
     1741 tctcttcgcc cctgcccata acatcgtggc gcgtgccgtg gatccacaac gccttcgaga
     1801 acgccgcggc cgtcatatcg ggctgcgaat caggcatgaa ggcattgatg cgcaagggca
     1861 agatccccgc gaagaagatc aacttcgtgg gcatgggcgg tgacggggcg acggcggaca
     1921 taggcatggg acccctctcc ggggcgctgg agagaggcca cgacatgatc tacgtgtgct
     1981 acgacaacga ggcctacatg aacacgggca tccagcgctc ctcgtgtacc ccctacggtg
     2041 cgagcaccac cacgagcccc gccgggaaga agagcatcgg gcagcacacc cagaagaaga
     2101 acatggtcga gatcgcagcg gcccacggga tcccgtacgt ggcgaccgca tgccccagct
     2161 tccccttcga cctcatggag aaggtgaaga aggcatccga gaccgcgggg cccgcgtaca
     2221 tccacatcct ctccgtgtgt cccacggggt ggaggatcaa tccctcggaa gccatcaagt
     2281 acgggcgcct tgcggtgaac tcctgcgttt tcccgctgta cgaggtcgac cgcgggaagt
     2341 accggctcac ctacaagcct tcggacaaga agctcatccc ggtctcggag tacctgaagg
     2401 gacagggcag gttcaggcac ctgaccgatt ccgacatcga gaccatccag gccgcaacca
     2461 tgaaggccta cgagcgcctc ctcaggctga gcgagcagga atgagggagt cctgaaggca
     2521 aggccggcaa ggaacaaagg cgtgcccggg acgtgtgctc agccctgaat ggaccgggct
     2581 gcccgggccg gccttgcgtt caaggctcga gtgcacggcc ggccatgagc gccgcccggt
     2641 ctcagagcga agggcgatgg accgcctctg ccggtaccat cgcccaggat tccgagcgtc
     2701 ggaaagttga cagctttccc cgatgcggct agcatatggc tccgccaagc tttgaggaga
     2761 tacgagtatg ctcgagctca taaccgatgg attccgcaag gcccaggaca tcatacaggg
     2821 caaggtcacc atccaggaaa agcacataga ggacgcgctc agggagatcc gcctgtcgct
     2881 gctcgaggcc gacgtggagt tccacgtggt caagggcttc atcgatcacg tcaaggaaaa
     2941 ggccataggc gagatcgtcc tgacaagggt ctctcacggc ggcaagaaac tgaaggtgac
     3001 gccggaacag cacttcgtga agatctgcta cgacgagctc gtgagactca tgggtccggt
     3061 ggacacctcg cttgccctgg gtaagaggcc tgtgggggcg gtcatgatgg tgggcctcca
     3121 gggcagcggg aagacgacga ccacggcgaa gatggcccga tacctccaga agaagggcaa
     3181 gaggcccctc atggtcgccg ccgacatcta caggccggct gcaatcgagc agctcaaggt
     3241 gctcgggtcg aggctcgaca taccggtgtt ccacgcgccc gggaagaccc cgccgcagat
     3301 ctgtaaagat gccatggagg cggccaggtt caaggggtgc gacgtggtcc tcttcgacac
     3361 ggccggccgc accatcgtgg acgacgccct catgcaggaa ctggaggaga tcaaggcatc
     3421 gacatcgccc gaggacatcc tcctcgtggt ggacgccatg atcggccagg aatcggtcaa
     3481 cgtcgcgaag gagttcgacc gcaggctcga cctctcgggg ttcgtcatca ccaagctcga
     3541 cggcgatgcg cgcgggggcg cagcactgtc cataaaggag gtcacgggca agcccataaa
     3601 gttcataggc atgggcgagt cgctggaccg catcgaggag ttcaggccag aagggctcgc
     3661 ctcgcggatc ctgggctacg gggacatcgt cggcctcgtg aaggacttcg aggaggtcat
     3721 cgacgagaag aaggcggaag aggacgcgga aaagctcttg aggggcgagt tcaccttcga
     3781 cgactttctc gcccagctca aggtcatgaa gaaaatggga tcgctctccg atatcataag
     3841 caagctcccc ctgggccagt tcgggttgcc ccaggggatg aaggtggacg accgggagct
     3901 cgtgagggtg gaggcgatca tccattccat gaccgtatac gagaggaacc acccggattg
     3961 cctcgacgag agccgcatcc agcgtatagc caaggggagc ggccagggtc gccagagggt
     4021 ggtggatctc atcgcccggt tcaggaccat gcggcaggtc atgcagggcc tgggcgtcgg
     4081 gaagaagaag gtcctgggaa ggctcgggat agaccccggg atcgctggcg cactcgagaa
     4141 gacggtcccc aagccgcaga agaagagcct ggtggacctg aaaaaacgca aggaaaagag
     4201 aaaatccatc aagaagtcca gaaagagaaa tcgttagacc agcgtgggga cggacggggc
     4261 ctcaatggct gtatcacaag acacgaacgc agagctcgag gtatttcgcc ggatcagcga
     4321 ggcggccgtc tcgctcgccc ctgacgtcga ggcggtggcc cgggccattc tcgacatcgt
     4381 ggtcgacgtc acgcctgcag agaacgcctc cctcatgatg ccgagcgcgg acgggaagag
     4441 gctcgagatc aaggcggccc gggggaggaa cgacaggaag ggcaggtatt ccgagaggtc
     4501 cctgggccag atcttcgcga tcggcgaggg catagccggc atggcggccg agacgttgac
     4561 gccggtcatg atccacgaca cctccgcaga tccccgcttt caggccagga gccagtccgt
     4621 gaggatcggt tcgctgttga gcctgcccat cgtctacaac cgcgacgagc tcgtgggggt
     4681 gctcaatctg agccacccga gggccggcgt gttcgaggac ggccacctca ggctcatgtc
     4741 ggcgcttttg gcaagcgctg ccctggcgat caggaacgcc aagctcatgc gggagataga
     4801 cgacatgaac cgcgagctca aggccgagct ctccctgacg gacaaggcga tgagcgagtt
     4861 cggcaggagc atcctgaagg tcttcaacca gatggcgatc ggcatcctca ccaccgatcc
     4921 cacgggcgcc atatccgcca tcaacaagaa ggcccaggag atgctgggca agcaggcggg
     4981 tgagaccatc gccgacgtgg tgccggtcga gatcgtgcac aaggtgttca ctgacaagga
     5041 gggcatgacg ctggacg
//
LOCUS       DAOSCR010000124         5046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10432_length_5046_cov_3.058706, whole genome shotgun sequence.
ACCESSION   DAOSCR010000124 DAOSCR010000000
VERSION     DAOSCR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_5046_cov_3.058706"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1595
                     /locus_tag="PLT69_04400"
     CDS             180..1595
                     /locus_tag="PLT69_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOJ13715.1"
                     /translation="MDANTKESGQSTKKEGGPKGRFPSAVTGRPHPAWSVSRASYTLA
                     RAYRAFSKDACLLRAASLTFYTVMSVVPVFAVMFSFAKGLGFRSILERQLRDNLYGQE
                     EVLTYIISFSERLLENVKGGVLAGVGVLVLLWTVLMMLGNMEESFNHIWNVKRPRSLA
                     RKFSDYLSVMLVGPVLLASSSSIAILAITRLGDIGGREGVGGIVGPLVALSLKAVPLV
                     LMWALFTFIYVFIPNRKVPLKPAIVSGLAASISFIALQRVYLGFQIGVSHYNAIYGSF
                     AAVPLFLTFTQLGWIIVLFGAELCHAMHDAGGFASLGPLDPFDANVAALAVVHRVVTN
                     YSNRGDSTTKDEMKKTAMIPGEVLEEILVRLEASGILLGVSCDEPSGRRYVPGRDPCG
                     ITACHVIEAVCGLLPQTAPHASSKLRDTAPASILLGLARDLEHSEAARPLKDLDIEGV
                     SRTANQAAEPEPGDPGGGSAG"
     gene            complement(1654..1995)
                     /locus_tag="PLT69_04405"
     CDS             complement(1654..1995)
                     /locus_tag="PLT69_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOJ13716.1"
                     /translation="MPIVFKTTALLVLSNVFMTFAWYGHLRNLSTAQWYVAAIASWGI
                     ALFEYMLQVPANRIGFTQMSLPQLKVLQEVVTLGVFVPFSMFYMRQPLKLDYLWAGLC
                     LVGAVYFIFRN"
     gene            2398..3855
                     /locus_tag="PLT69_04410"
     CDS             2398..3855
                     /locus_tag="PLT69_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13717.1"
                     /translation="MTVPELDHEAIVRLNTEELGSASDGIPWVSAEGARRSEETRKGL
                     LARLGRGVSYSFKGSKPHLGPLSPGCTLCGEGSWSCLFVNNLCNASCFFCPAPQDDAD
                     MPGTSTLTFSDPLEYAEYVERFGFRGVSISGGEPLVHPERTLSFIEAVRRRLGGDVYL
                     WMYTNGKLLSRELASRLRDAGLDEIRFNIAASGYDLEAVRTALKIIPTVTVEIPAVPE
                     DLETLEKISRTLSDLGVRHLNLHQIRCTRFNKDRLAKRGYTFLHGPLSAVLESELAAL
                     SFLAYAQDTGLAIPVNYCSAIYRHRFQARASRRRWARYVAKPFETVTEAGLIRTVHLE
                     GEGRVLSSVQESLRAGGTDGRRWDVSNKGRRMSIDADLLGLIHTDGLEVKVAYSLATI
                     RPTHSYLHPFTEITLSSGRTLVIERATCLGATALDAGELRLFTESFPGGGAAAMEADL
                     VYERAARISGDPSRVAAWKRIIQAESIRSGLLEYY"
     gene            complement(4179..>5046)
                     /locus_tag="PLT69_04415"
     CDS             complement(4179..>5046)
                     /locus_tag="PLT69_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13718.1"
                     /translation="VMVGLPGDGPGDVMRAVRFVLDNGLCDELAMHPLCVLPGTVLRS
                     QAEALGIEYMEDPPYLLTRTPTMSAADVREVISWCEDALCTDLFPVEMPLMAGGAPVR
                     GDRRGCFVQRIVLAGAGEGVTCEPSRIGQALCIELREAAWLEREDLSCSLKTLLSENP
                     YTLVSWIVPERLFVPGKTLERIKALSHRAPHPIDRDYMSTYTPVRSLQVFLEVETRPG
                     GPSYVMVPLERDPSRPLWAGLPRDAGGDEEERVQGIVSSLVGEGLTVRFHDLEPREPG
                     PIAERLCTLVVG"
BASE COUNT          880 a         1708 c         1631 g          827 t
ORIGIN      
        1 cgcacgcaac cgcagtccgg atgaattgaa tgatcaggag gccctggacc tcttggggct
       61 acccaacccg gggcgggcgt ggtaagaaga tggcggaccg agggaccgag acacccacgc
      121 ctgaaacccg tgcgccatga gcttccctcg atacccccac ctcttgtgga gacgggccca
      181 tggacgcgaa caccaaagag agcggacaga gcacgaagaa agagggcggc cccaaagggc
      241 gtttcccgtc ggccgtcaca ggccgtcccc acccggcgtg gtcggtctcg cgcgcctcct
      301 acaccctcgc gagggcgtac agggcgtttt cgaaagacgc gtgcctgctg cgggcggcct
      361 ccctcacctt ctacaccgtc atgtcggtcg tgcccgtctt cgccgtcatg ttctccttcg
      421 ccaagggact cgggttcagg tcgatcctcg agcgccagtt gagggacaac ctctacggcc
      481 aagaggaggt gctcacctac atcatctcct tctcggaaag gctcctggag aacgtcaaag
      541 gcggcgttct cgcgggggtg ggcgtcctgg tgctcctgtg gaccgtcctc atgatgctcg
      601 gcaacatgga ggaatcgttc aaccacatct ggaacgtgaa gcggccgcga agcctcgcga
      661 ggaagttcag cgactacctc tcggtcatgc tggtgggccc cgtccttctc gcgtcctcga
      721 gcagcatcgc cattctcgcg atcacgcgcc ttggcgacat cggcggccga gaaggcgtcg
      781 gcgggatcgt gggcccgctc gtcgccctgt cgctgaaggc cgtacccctg gtgctcatgt
      841 gggcgctgtt caccttcata tacgtcttca tccccaacag gaaggtgccg ctcaagcccg
      901 ccatcgtctc cgggctcgcc gcgtcgatct cgttcatcgc gctgcagagg gtctacctgg
      961 gattccagat aggcgtgtcg cactacaacg ccatctacgg gagcttcgcc gccgtgcccc
     1021 tgttcttgac cttcacccag ctgggatgga tcatcgtgct cttcggcgcc gagctctgcc
     1081 acgccatgca cgatgccgga gggttcgcgt ccttagggcc cctggacccg ttcgacgcca
     1141 acgtggcggc gttggccgtc gtgcaccggg tggtgacgaa ctactcgaac cggggcgact
     1201 cgacgaccaa agacgagatg aaaaagacgg cgatgatccc gggcgaggtc ctcgaggaga
     1261 tcttggtccg ccttgaagcc tcggggatac tcctgggagt ctcttgcgac gaaccctcgg
     1321 gaagacgcta cgtgccgggc cgcgacccgt gcggcatcac cgcctgtcac gtcatcgagg
     1381 ccgtctgcgg cctgctgccg cagacggccc cgcatgcctc ctcgaaactc agggacacgg
     1441 cgcccgcatc catccttctg ggccttgcgc gtgatctcga gcactctgag gcggcgaggc
     1501 cgctgaagga cctcgacatc gagggcgtct ccaggacggc gaaccaggcc gccgagcccg
     1561 agcccggcga tccaggcggc gggagcgcag ggtagggggt ggacggccgt ccttccacac
     1621 tcgcgcgcat gcgcacagga cggcccggtt cgactagttc ctgaagatga agtacacggc
     1681 gcccaccagg cacaggcccg cccacaggta gtcgagcttc aggggctggc gcatgtagaa
     1741 catcgagaac ggcacgaaga ccccgagcgt gaccacctcc tggaggacct tgagctgcgg
     1801 gaggctcatc tgggtgaagc cgatgcggtt cgcggggacc tggagcatgt actcgaacag
     1861 ggcgatgccc cagctcgcaa tggccgcaac gtaccactgg gccgtgctca ggtttctgag
     1921 gtgcccgtac catgcgaagg tcatgaagac gttcgagagc accagcagcg ccgtggtctt
     1981 gaacacgatg ggcatgatga gatatctccc gttgcagtgt gtttcccggc cgaccctcta
     2041 ccagaagtcc ccggcctcgt tcaactggta tttcggcagg aagaccggcg cactcgagac
     2101 accggggtca cactggattt tcccgggacg tcgagcctgg ctctcgagcg cccggcgaag
     2161 cgggaacccc cctcgcccca ggcacgctgt ggatccgttc gcgttttcgg gtgagggagt
     2221 cttgacaaag acatcctgca gccgaaaacc ccacaagcgg gcggttcctc ccgcggccag
     2281 ggtcgagcgt cgccggagcg ccctgaaggg ctgcatcctg ttgaccaggg ccggtttttt
     2341 ccctataggg ggtgtccgga agacacgaga aggaacgccg atgtgagggt gtctctcatg
     2401 acggttcccg aactcgacca cgaagcgatc gtacggctca acacagaaga actcgggtcc
     2461 gcatccgacg gcatcccgtg ggtctcggca gaaggcgccc gcaggagcga ggagacgagg
     2521 aagggactcc tggcaaggct cggccggggc gtatcgtact ccttcaaggg ttcgaagccc
     2581 cacctggggc cgctttcacc cgggtgcacg ctctgcggag aggggtcgtg gtcgtgcctc
     2641 ttcgtcaaca acctgtgcaa cgcctcgtgc ttcttctgcc ccgcccccca ggacgacgcg
     2701 gacatgccgg gcacctcgac cctcaccttc tcagaccccc tggaatacgc cgaatacgtc
     2761 gagcggttcg ggttcagggg cgtgagcatc agcgggggag agccgctggt gcaccccgag
     2821 aggaccctct ccttcataga ggccgtgagg cgaaggctcg ggggcgatgt gtacctgtgg
     2881 atgtacacca acgggaagct gctcagcagg gaactcgcct cccgcctgag ggacgcgggt
     2941 ctcgacgaga tccgcttcaa catcgcggcc tcgggctacg acctcgaggc cgtgcggaca
     3001 gctctaaaga tcatcccaac ggtgacggtc gagatacctg ccgtgccgga ggacctcgag
     3061 accctcgaga agatctcccg gacgctttca gacctcggcg tcaggcacct caacctccac
     3121 cagatcaggt gcacgaggtt caacaaggac aggctcgcga agaggggata caccttcctc
     3181 cacggaccgc tctcagcggt cctcgagtcg gagctcgcgg ccctgtcgtt cttggcgtac
     3241 gcacaggaca cgggcctggc gatcccggtc aactactgca gcgcgatcta taggcacagg
     3301 ttccaggcgc gggcatcgag gaggcgctgg gcgcggtacg ttgccaagcc cttcgagacc
     3361 gtgaccgagg cggggctgat ccgcaccgtg cacctcgagg gggaaggccg cgtcctctcc
     3421 tcggtccagg agtctctacg cgccggcggg acagatggcc gccggtggga tgtctccaac
     3481 aaggggagac gcatgagcat cgacgccgat ctcctgggcc tcatccacac cgacggcttg
     3541 gaggtcaagg tcgcctacag cctggccacg atacggccga cccactcgta cctgcacccc
     3601 ttcaccgaga tcaccctctc ttccggcagg acgctcgtca tcgagagggc cacgtgcctg
     3661 ggcgcgacag cgctggacgc aggtgagctc cggctcttca ccgagagctt ccctggcggg
     3721 ggcgcggccg caatggaggc ggacctcgtg tacgaaaggg ccgcacggat ctccggggac
     3781 ccttcgcggg tcgcggcctg gaagaggatc atccaggcgg agtccatcag gagcggcctc
     3841 ctcgagtact actgaccgcg ctcctcatgc cgcccattca ccggccgacc gcacccaccg
     3901 ggcccggttg tcgtggacga ggaagcatac gtcctcgagc ggggaaagag cccggtcggc
     3961 ccgtcgtcgt gcccggctcg gtcctccccc cgccgggtca ggccccaggg atggtccttg
     4021 ggcctgcctg acacccgcac aaccggctcg ccgcccccca cgaaccgacc cggccctgtg
     4081 cccgggcgtc acatgtcggg gtgccaagcg gttttctccg cgatggtgag cgtgccgagc
     4141 cggacttaag tcctggcacc ccacggccgc ctgcgccctc atccgaccac cagggtgcag
     4201 agccgctcgg cgatggggcc gggctctcgg ggttcgaggt catggaagcg cacggtgagg
     4261 ccttcgccca cgagcgagga gacgatcccc tgcaccctct cttcctcgtc gccgcctgcg
     4321 tctcgtggga gtcccgccca caggggacgc gaggggtcgc gctcgagcgg caccatgacg
     4381 taggacgggc ctcccggtcg cgtctcgacc tcgagaaaga cctggaggga acgcacgggc
     4441 gtgtaggtgg acatgtagtc ccggtctatg gggtgaggcg ccctgtggct cagggccttt
     4501 atccgttcca gggtcttccc cggcacgaac aggcgctcgg gcacgatcca gctcaccagg
     4561 gtgtaggggt tctcggagag cagggttttc agcgagcagg agaggtcttc ccgctcgagc
     4621 caagcagcct ctctcagctc gatgcacagg gcctggccta tgcgcgatgg ctcgcaggtc
     4681 accccctctc ccgcgccggc gagcacgatc ctctgcacga aacacccccg cctgtcccct
     4741 cgcacggggg cgccgcccgc catgagcggc atctccacgg ggaagagatc ggtgcacagc
     4801 gcgtcctcgc accacgagat cacctctcgc acgtccgcgg cgctcatggt cggggtgcgc
     4861 gtcagaaggt agggcggatc ctccatgtac tcgatgccga gcgcctcggc ctgagacctg
     4921 agcaccgtgc ccggcagcac gcacaagggg tgcatggcca actcgtcgca caggccgttg
     4981 tcgagcacga aacgcacggc tcgcatcacg tcgcccgggc cgtcgcccgg caggcccacc
     5041 atgacg
//
LOCUS       DAOSCR010000125         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10482_length_5033_cov_4.428686, whole genome shotgun sequence.
ACCESSION   DAOSCR010000125 DAOSCR010000000
VERSION     DAOSCR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10482_length_5033_cov_4.428686"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1167)
                     /locus_tag="PLT69_04420"
     CDS             complement(337..1167)
                     /locus_tag="PLT69_04420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ13719.1"
                     /translation="MTQYQGSEKQVASGDRFAFGANWSRFLKTLNDDRILQAETSLKE
                     MLETQDLTGRSFLDIGSGSGLSSLAARRLGARVHSFDYDPQSVACTKELKRRYFPDDE
                     LWRIELGSILDDRYVSSLGKWDIVYSWGVLHHTGDLWKAMEHAVSLVDKGGSVFIAIY
                     NDQGYLSSRWARLKYLYNKKPWTRPILLAYGLVHFWGRTFLKEFIALRPFETWKAHYK
                     NRGMSAWHDLVDWMGGWPYEVATPDAVFHFCRDRGFTLRRLVTRQGIGNNEFVFVRNA
                     "
     gene            complement(1145..2263)
                     /locus_tag="PLT69_04425"
     CDS             complement(1145..2263)
                     /locus_tag="PLT69_04425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ13720.1"
                     /translation="MLVVSSLSAGGAERVVAEIANWWSKKGRKVTVITLAGKEHDHYK
                     LDPPVGRVALDIWGRTFIPWFVIGKGIKRYTKIRGHIRGLSPDIVVSFMDTNNVRTLI
                     ALAGTGVPVIACERTDPRHHDIGLYWSFMRRVTYPLCSALVVQTEAVAASWARRVVPA
                     RKVAVIPNFVRTMPETEETGPLHDEPVILTVGRLGREKGHDLLIRAFAALGEVRTGWR
                     LVILGEGKERKNLERLASSLGIQDSVLMPGVVPEPAHWLYKAPIFVLPSRYEGFPNAL
                     LEAMAAGCAVIAADCPSGPAEIVKNDHNGILVPTEDVPALCAAMKRLILAKDLRDHLG
                     RNAAHVSSTFSQHTIMQAWDSLVDHYLQRREYDAVSRQ"
     gene            complement(2406..3575)
                     /locus_tag="PLT69_04430"
     CDS             complement(2406..3575)
                     /locus_tag="PLT69_04430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ13721.1"
                     /translation="MTAGLPHRPGRILLSILSLQPGGAERVISEMANWWAARGLAVGV
                     MTLGGGKNDHYELAPGVVRYRVDSWRHASSRLARWADLALLPFAVRKTVRSFAPEVVV
                     SFIDLVNNIMAFSLAGSGVPLVVCERIDPRHHRISKARALARRVLYPLACALVVQTEA
                     VAASWARRVVPAHKVAVIPNFVRDLPPRSACPGPQGVSSPYVLAVGRLDAQKGHDLLI
                     RAFAKVSRDIPGWRLVILGEGPERHTLSRLASSLGMSDSVMMPGVVPEPAAWMYGASL
                     FVHPSRYEGFPNALLEAMAAGCAVIAADCPSGPAEIVRHGVNGVLVPSDDLESLGTAM
                     KTLMEDTKARERLGKEALKVAETFSSAAVMGAWDELLSRVTRKGDVQDGGPPWKR"
     gene            complement(3572..4681)
                     /locus_tag="PLT69_04435"
     CDS             complement(3572..4681)
                     /locus_tag="PLT69_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ13722.1"
                     /translation="MPERLLLATGSLTAGGAERVCSEMANWWCGQGRHVAVMTTENAY
                     PDHYSLARGVRRITFDWQLPRTRLHIPWQHVKNKLTVRRAVLEFSPGAVISFMDTNNV
                     RTLIALAGTGVPVIACERTDPRHHDIGLYWSFMRRVTYPLCSALVVQTEAVAASWARR
                     VVPARKVAVIPNFVRTMPETEETGPLHDEPVILTVGRLGREKGHDLLIRAFAALGEVR
                     TGWRLVILGEGKERKNLERLASSLGIQDSVLMPGVVPEPAHWLYKAPIFVLPSRYEGF
                     PNALLEAMAAGCAVIAADCPSGPAEIVKNDHNGILVPTEDVPALCAAMKRLILAKDLR
                     DHLGRNAAHVSSTFSQHTIMQAWDSLVQRVCRCAR"
     gene            complement(4674..>5033)
                     /locus_tag="PLT69_04440"
     CDS             complement(4674..>5033)
                     /locus_tag="PLT69_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ13723.1"
                     /translation="GYDTFVGERGALLSGGQKQRIAIARVFLKNPNILILDEATSALD
                     EKNEQAIQNTINTLKERITLIIIAHRLTTVEGCDRVVWLDKGRVRLVGDAKTVIAQFR
                     RQSGEAPEIEGSRGGDA"
BASE COUNT          812 a         1633 c         1629 g          959 t
ORIGIN      
        1 atcccgcaca cgtctcgctc gctcggcctc ctgcttcgat gacacccgac cgggtcgtca
       61 cgtccctcac caggtgctcg cctgcagcca ccgccggctt cggcaatgca ccgcccggtc
      121 gtcctcgatc gtttgttctc ccgtcgattt cacgatcctg cacggattcc ttggggtttg
      181 aggcgatgcg tatccgggaa cgtggagaca ggcatgcaag ggcgccttca ggcacgaggt
      241 ggccgtccgt accatgcagg cggacatgcc gagatgttcc gggtgttctt atgcccctgg
      301 ttggccaggg tgcgaaccga cagcagggtg acccgatcac gcgtttctca caaagacgaa
      361 ctcgttgttc cctattccct gcctcgtcac gagtctcctc agggtgaacc ccctgtcccg
      421 gcagaagtga aacacggcat ccggggttgc gacctcgtag ggccagcccc ccatccagtc
      481 aacgaggtcg tgccaggcag acatgcctcg attcttgtag tgcgccttcc acgtctcgaa
      541 gggtctcagg gcgatgaact ctttcaggaa ggtcctcccc cagaaatgca ccaggccgta
      601 cgcgaggaga atcggcctcg tccagggttt cttgttgtag agatatttca atctcgccca
      661 tcgagaggag aggtaaccct ggtcgttgta tatggcaatg aagaccgacc cacccttgtc
      721 caccagcgag acggcgtgtt ccatggcctt ccagaggtcc ccggtgtggt ggagaacgcc
      781 ccatgagtac acgatgtccc atttcccgag agaggagacg tagcggtcgt ccaggatgga
      841 tcccagctct atccgccaca gctcgtcgtc gggaaagtac ctgcgcttga gctccttcgt
      901 gcatgcgacc gactgcgggt cgtagtcgaa cgagtgaacc ctcgcaccca ggcgcctggc
      961 cgcaagggaa gaaagaccgc ttccgctgcc gatatccaag aacgacctgc ccgtgagatc
     1021 ttgggtctcg agcatttcct tgagggaggt ctccgcctgc aagatcctgt cgtcgttgag
     1081 cgttttcagg aacctgctcc agttggcgcc gaaagcgaaa cgatcgccgg atgcgacctg
     1141 tttttcactg ccttgatact gcgtcatatt ccctcctctg gagataatgg tcaacgaggc
     1201 tgtcccatgc ctgcatgatg gtgtgttggg agaaggtgga ggagacgtgg gcggcgttgc
     1261 ggccgaggtg gtctctgagg tctttggcga ggatgaggcg tttcatggcg gcacagaggg
     1321 ccgggacgtc ctcggtgggg acgaggatgc cgttgtggtc gttcttgacg atctccgcgg
     1381 gcccgctcgg gcagtcggcc gcgatcacgg cgcagcctgc ggccatggcc tcgagcaggg
     1441 cgttggggaa tccctcgtag cgggaaggga ggacgaagat gggggccttg tagagccagt
     1501 gggccggttc cggcacgaca cccggcatga ggacggagtc ctggatgccg aggctggatg
     1561 cgagccgctc gaggttcttc ctttcctttc cctctccgag gatgacgagt ctccagcccg
     1621 tgcggacctc tccgagggct gcgaaggccc tgatgaggag gtcgtggccc ttttccctcc
     1681 cgagccttcc cacggtgagg atgacgggct cgtcgtggag agggccggtt tcttcggtct
     1741 cgggcatggt gcggacgaag ttggggatca cggccacctt gcgggcgggg accacccttc
     1801 ttgcccacga tgccgccacc gcctcggtct ggaccaccag ggcgctgcac aaggggtagg
     1861 tgacgcgcct catgaacgac cagtagaggc cgatgtcgtg gtgcctcggg tcggtgcgct
     1921 cgcacgcgat caccggcacc ccggtccccg caagcgcaat caacgtccgc acgttgttcg
     1981 tgtccatgaa gctgacgacg atgtcggggg acagcccccg tatgtgcccc ctgatcttgg
     2041 tgtacctctt gatccccttg ccgatcacga accacgggat gaacgtcctg ccccagatgt
     2101 cgagcgcgac cctcccgaca ggggggtcga gcttgtagtg gtcgtgctcc ttgcccgcca
     2161 gggtgatgac ggtcaccttc ctgcccttct tggaccacca gttcgcgatc tcggccacca
     2221 cgcgttccgc ccccccggcg gagagggaag agacgaccaa cacgatatgc ccctcaggag
     2281 aaggttgttc ccgaggcgct cctgtcatcc cggtcgaccg gtgcgatccc atccttgccc
     2341 tcgcgtcatg aacattcggc tctccctgcc ccttcagcac aagagcgttc ggggacggcc
     2401 cgccatcacc ttttccatgg tgggccgcca tcctgcacat cccctttcct tgtcacccgg
     2461 gacaagagtt cgtcccatgc gcccatgacg gcggcgctcg agaaggtctc ggccaccttg
     2521 agcgcctctt tgccgaggcg ttcccgggct ttcgtgtctt ccatgagcgt cttcatggcg
     2581 gtgccgagcg actcgaggtc gtcgctcggc acgagcactc cgttgacgcc gtgtcttacg
     2641 atctccgcgg gcccgctcgg gcagtcggcc gcgatcacgg cgcagcctgc tgccatggcc
     2701 tcgagcaggg cgttggggaa gccctcgtag cgggaggggt ggacgaacag ggacgcgccg
     2761 tacatccagg ccgcaggctc cggcacgaca ccgggcatca tgacggagtc gctcatgccg
     2821 aggctggatg cgagccggga gagggtgtgc ctctccggcc cctcgcccag gatcacgagt
     2881 ctccacccgg ggatgtccct cgacaccttc gcaaaggccc tgatgaggag gtcgtgaccc
     2941 ttctgcgcat cgagccttcc cacggccagc acgtacgggc tcgagacccc ctggggccca
     3001 gggcatgcgg accggggggg gaggtcgcgc acgaagttgg ggatcacggc caccttgtgg
     3061 gccgggacca cccgtctggc ccaggatgcc gccaccgcct cggtctggac caccagggca
     3121 caggcgaggg ggtagagaac gcgccgtgcg agcgccctcg ccttgctgat ccggtggtgc
     3181 ctcgggtcga tccgctcgca cacgacaagc gggactccgc ttcccgcaag cgagaaggcc
     3241 atgatgttgt tcacgaggtc tatgaagctg acgaccacct caggcgcgaa cgacctcacg
     3301 gtcttcctca cggcgaacgg cagcagggcg aggtcggccc acctggcaag acgcgacgac
     3361 gcatgccgcc aggaatcgac ccggtacctc acgacgccgg gggccagctc gtagtggtcg
     3421 ttctttcccc ccccgagcgt catgaccccc accgcgagcc cccttgcggc ccaccagttc
     3481 gccatctcgc tgatcacccg ctcggccccg cctggttgca acgacaggat cgacagcagg
     3541 atgcgcccgg gtcgatgggg aaggcctgca gtcatctggc gcaccggcac acccgttgta
     3601 cgaggctgtc ccatgcctgc atgatggtgt gttgggagaa ggtggaggag acgtgggcgg
     3661 cgttgcggcc gaggtggtct ctgaggtctt tggcgaggat gaggcgtttc atggcggcac
     3721 agagggccgg gacgtcctcg gtggggacga ggatgccgtt gtggtcgttc ttgacgatct
     3781 ccgcgggccc gctcgggcag tcggccgcga tcacggcgca gcctgcggcc atggcctcga
     3841 gcagggcgtt ggggaatccc tcgtagcggg aagggaggac gaagatgggg gccttgtaga
     3901 gccagtgggc cggttccggc acgacacccg gcatgaggac ggagtcctgg atgccgaggc
     3961 tggatgcgag ccgctcgagg ttcttccttt cctttccctc tccgaggatg acgagtctcc
     4021 agcccgtgcg gacctctccg agggctgcga aggccctgat gaggaggtcg tggccctttt
     4081 ccctcccgag ccttcccacg gtgaggatga cgggctcgtc gtggagaggg ccggtttctt
     4141 cggtctcggg catggtgcgg acgaagttgg ggatcacggc caccttgcgg gcggggacca
     4201 cccttcttgc ccacgatgcc gccaccgcct cggtctggac caccagggcg ctgcacaagg
     4261 ggtaggtgac gcgcctcatg aacgaccagt agaggccgat gtcgtggtgc ctcgggtcgg
     4321 tgcgctcgca cgcgatcacc ggcaccccag tccccgcaag cgcaatcaac gtccgcacgt
     4381 tgttcgtgtc catgaagctt atgactgcgc ctggggagaa ctcgagaacc gccctgcgca
     4441 cggtgagctt gttcttgacg tgctgccagg ggatgtgcag gcgggtgcgc ggcagctgcc
     4501 agtcgaaggt gatccgcctg acgcctcggg ccaggctgta gtggtcggga taggcgttct
     4561 cggtggtcat gaccgcgacg tgccggcctt ggccgcacca ccagttggcc atctcgctgc
     4621 agacacgctc cgcgcccccc gctgtcaggg agcccgtcgc cagcagcaag cgctcaggca
     4681 tccccccccc gactgccctc gatctcgggt gcctcaccgc tctgtcgcct gaattgagct
     4741 atcacggtct tcgcgtcgcc cacgagcctg accctgccct tgtccagcca caccaccctg
     4801 tcgcagccct ccacggtggt gagcctgtgg gctatgatga tgagcgtgat cctttccttc
     4861 agcgtgttga tggtgttctg gatggcctgc tcgttcttct cgtcgagggc gctcgtcgcc
     4921 tcgtccaaaa tgaggatatt ggggtttttc aaaaacaccc tcgcgatggc aatgcgctgc
     4981 ttttgcccgc ccgacagcag cgcccccctt tctcccacga aggtgtcgta gcc
//
LOCUS       DAOSCR010000126         4970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10671_length_4970_cov_2.950763, whole genome shotgun sequence.
ACCESSION   DAOSCR010000126 DAOSCR010000000
VERSION     DAOSCR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10671_length_4970_cov_2.950763"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLT69_04445"
     CDS             complement(<1..321)
                     /locus_tag="PLT69_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOJ13724.1"
                     /translation="MVGTLANTASVIVGSTIGIMAGRYMGADIKRTLMHALGLAVMVI
                     GLKMALDATTLIPSVACLLLGALTGEAIRIEHAVERLGEALKARFAQGSKTFVEGFVS
                     ATVLF"
     gene            654..1805
                     /locus_tag="PLT69_04450"
     CDS             654..1805
                     /locus_tag="PLT69_04450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ13725.1"
                     /translation="MPRIGRVVVQNLWEAFVTNEADQSLRIKRFLMAFASYCMWCAIV
                     WYFYTQGLSRLSFEGSMIMTAAALLTNAAFYAVLRSGLNKRLKDPSMTLAQMMFATLW
                     IMVVAYIVKDARGIMLLLYLVVFIFGAFRLTLGEFLRLSVFASAGYALVVFSLHRYHP
                     DEVNLKVELINLLVLSSVLFWFSFVGSYINKLRRRLNTANRELKDAMEIIRFRSAHDD
                     LTGVYNRGHLFRILKREKGLADREGTTFCLCIFDIDDFKAVNDTFGHRAGDTVLETLA
                     CMLMDNIRLQDYLARYGGEEFVIVLAYPTIQGAAACAERLRRLVSNLSFEGLPEDFRV
                     TVSMGLTRYISPESVDDLLKRADRALYEAKRSGKNALVTDPALRKTSAA"
     gene            complement(1813..2463)
                     /locus_tag="PLT69_04455"
     CDS             complement(1813..2463)
                     /locus_tag="PLT69_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ13726.1"
                     /translation="MRAVIFDMDGTLLDTIEDIARSMNEALGLLGFPAHPVDRYLDFV
                     GDGIDALARRALPGDVSDDALVHKAVLEMRRIYASSWACTTRPFAGVPEMLDVLAARG
                     VKMAILSNKLDAFTKDMAATLLPRWRFEVVQGLEPGLARKPDPEGALRCARLMGVAGA
                     ECAFVGDSVIDILTGKSAGMMTVGVLWGYQDGARLASAGADALVAHPSELPDALLS"
     gene            complement(2864..3421)
                     /locus_tag="PLT69_04460"
     CDS             complement(2864..3421)
                     /locus_tag="PLT69_04460"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOJ13727.1"
                     /translation="MNKVKQGARTFLFPMPSVLVGATVDGKPNFMTVAWCGIASHQPP
                     TVAVAIRKGRHTLKGIEAHHCFSVNVPTSKMVKLVDFCGIYSGKDRDKANLFTVKPGD
                     NPLVPLVDECPVNLECTLLHTLDLGSHTLVVGQITQSHVREDCIEGKKIDPLNVDPLI
                     YATSSETYHRLGEVVGNAFSVGKEF"
     gene            complement(3418..3918)
                     /locus_tag="PLT69_04465"
     CDS             complement(3418..3918)
                     /locus_tag="PLT69_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOJ13728.1"
                     /translation="MRKARTNTGGEPFAELEGRSYFFDHGLMFGCTRCGACCTGEPGV
                     VYLTRREAGRIARFLGIPADVLEARMLEPFKDGFTVREAEDGRCIFYENGCVIYPERP
                     IQCATFPFWFQNLRSSRAWKEAGRRCPGIGRGRLFSKEEIIACVHASFPVYLKVAEFI
                     STGGSR"
     gene            4134..4307
                     /locus_tag="PLT69_04470"
     CDS             4134..4307
                     /locus_tag="PLT69_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ13729.1"
                     /translation="MAYVITDECISCGTCAEECPVDAISEGEDKYVIDPELCTDCGTC
                     AEACPVDAIEPGE"
BASE COUNT          857 a         1748 c         1482 g          883 t
ORIGIN      
        1 gaagagaacg gtggcgctca cgaaaccctc gacgaaggtc ttcgaaccct gggcgaacct
       61 ggccttcagc gcctcgccca gacgctccac cgcatgctcg atgcggatcg cctcccccgt
      121 caacgccccg aggaggagac aggccacgga agggatgagc gtcgtcgcgt cgagcgccat
      181 cttgagcccg atgaccatga ccgcgagccc caaggcgtgc atgagcgtcc gcttgatgtc
      241 ggcccccatg tacctgcccg ccatgatgcc gatcgtggag cccacgatca cggaagccgt
      301 attcgcaagg gttcctacca cgggtgcccc tctctgtgcg tcgatatcga accggatgac
      361 tccctgtacc ctcacccgtc ccaagcggtc aacctggaaa atccggcctc atggtccccc
      421 tgcggcgcct tcttgcgcca gacctcgccg accgtcgaac cgtcacgggc gccccctgct
      481 ccacgatcga gcgcttccgg cacggacgcg ccccccgcac gcccccgccc ctgcaccgcc
      541 cgcgcgatct cgtaggctaa ggcgcacaac cacgcaggcc ccagggcgcc cgacaggtcg
      601 ttctcatgat catgccggga tgtgacgaac agtgtttaca ggagctcgag acgatgccga
      661 ggatagggcg ggtggttgtg cagaacctgt gggaagcgtt cgtgacgaac gaggccgacc
      721 agtcgttgag gatcaagcgg ttcctcatgg ccttcgcttc gtactgcatg tggtgcgcca
      781 tcgtatggta cttttacacc cagggcctct cgcgcctgag cttcgagggg tccatgatca
      841 tgaccgccgc cgccctcctc acgaacgcgg cgttctacgc ggtgctcagg agcgggctca
      901 acaagaggct caaggacccg agcatgacgc tcgcccagat gatgttcgcc acgctctgga
      961 tcatggtggt cgcctacatc gtgaaggacg cgaggggcat catgctcctg ctctacctcg
     1021 tggtcttcat tttcggcgcc ttccgcctca ccttgggcga gttcttgaga ctgtccgtgt
     1081 tcgcctccgc tggatacgca ctcgtggtgt tctcgcttca ccgctaccac ccggacgagg
     1141 tgaatctcaa ggtcgagctt atcaacctcc tggtgctctc ctcggtgctg ttctggttct
     1201 ccttcgtggg gagctacatc aacaagctca ggaggaggct caacaccgcc aaccgcgagc
     1261 tcaaggacgc catggagatc atcaggttcc gctcggccca cgacgaccta accggcgtgt
     1321 acaaccgcgg ccaccttttc aggatcctca agagggaaaa ggggctggct gacagggagg
     1381 gcaccacgtt ctgcctgtgc atcttcgaca tcgacgactt caaggccgtc aacgacacgt
     1441 tcggccaccg tgccggcgac acggtcctcg agaccctggc ctgcatgctc atggacaaca
     1501 tccggctcca ggactacctg gcacgctacg gtggagagga gttcgtcatc gtcctggcct
     1561 accccacgat acagggcgcc gccgcctgcg cagagcgcct gcgccgcctg gtctcgaacc
     1621 tctcgttcga gggtctgccc gaggacttcc gcgtaaccgt gtccatgggc ctcaccaggt
     1681 acatcagccc cgagagcgtc gacgacctcc tgaagagggc cgacagggcc ctgtacgagg
     1741 cgaaacgatc gggcaagaac gccctggtca cggacccggc cctgaggaag acatccgctg
     1801 catgagggcc gctcaggaga ggagcgcgtc gggcagttcc gaggggtggg ccacgagcgc
     1861 gtcggcgccc gccgaggcca gccgcgcccc atcctggtaa ccccacagga cgccgacggt
     1921 catcatgccg gcggactttc ccgtgaggat gtcgatgacg ctgtcaccca cgaacgcgca
     1981 ctccgcgccc gccacaccca tgagcctggc gcacctgagg gcgccctcgg gatcgggttt
     2041 cctcgccagc ccgggctcga gcccctgcac cacctcgaac ctccacctcg gcaggagggt
     2101 cgccgccatg tccttggtga acgcgtcgag cttgttggac aggatggcca tcttcacccc
     2161 ccttgccgct aggacgtcga gcatctcggg caccccggcg aagggtctcg tggtgcaggc
     2221 ccacgacgag gcgtagatcc ttcgcatctc caagaccgcc ttgtgcacga gggcgtcgtc
     2281 tgagacatcc ccgggcaggg cgcgtctcgc cagtgcgtcg atcccgtcac ccacgaagtc
     2341 gagatacctg tctaccgggt gggcggggaa acccagcagc ccgagcgcct cgttcatgga
     2401 ccgggcgatg tcctcgatgg tgtccaggag cgttccgtcc atgtcgaaga tcactgcccg
     2461 catgtattca taccccctgc cttcctcgat ctcggttcgg gcgagcgtcg cgggcgtgcc
     2521 tgcacgccgg gtgcgctccc cgccccctgg atgaagcccc tctcgtgcct ttctccaaaa
     2581 acacagatat ccttgcgacc ctgtcctgtc ccaagccttg ccgcaggcct tctgagcgag
     2641 gcaggctcga ggcctgatgc ggattccacc atccaccatc ccaccgggcg ccacgccttc
     2701 tcgcggggca tgccttgcat ggcctcgagc gtacaacgac cccgtgcacg gcggcctcgg
     2761 ttgcacacgc accatcgccg ggggccgcct tccccggacg gcgtgtcccg ctcccgcgag
     2821 agaggccccc tgctcccgcc agggggccga ggacaaagac ccctcagaac tccttgccca
     2881 ccgagaaggc gttgcccacg acctcgccga gacgatggta cgtctccgag gaggttgcgt
     2941 agatgagggg gtctacattg aggggatcga tctttttccc ctcgatgcag tcctcgcgca
     3001 cgtggctctg ggtgatctgg cccaccacga gggtgtgcga accgaggtcg agcgtgtgca
     3061 ggagcgtgca ctcgaggttg accgggcact cgtccacgag cggaacgagg gggttgtcgc
     3121 cgggcttcac cgtgaagagg ttggccttgt cgcgatcctt tccggagtag atcccgcaga
     3181 aatccaccag cttcaccatc ttgctcgtgg ggacgttcac cgagaagcag tggtgggcct
     3241 cgatcccctt cagcgtgtgg cgtcccttgc ggatggcgac ggccacggtc ggcggctggt
     3301 gcgaggcgat cccgcaccag gcgacggtca tgaagttggg cttgccgtca accgtcgccc
     3361 ccacgaggac ggacggcatg ggaaacaaga acgtacgcgc cccctgcttg accttgttca
     3421 tctcgaacct cctgtggata tgaattccgc aaccttgaga tagaccggga agctggcgtg
     3481 gacacaggcg atgatctcct ccttggagaa caggcgcccc ctgccgatgc cggggcacct
     3541 cctgcccgcc tccttccacg cgcggctcga cctgagattc tggaaccaga aggggaacgt
     3601 ggcgcactgt atgggcctct cgggatagat cacgcagccg ttctcgtaga agatgcaccg
     3661 gccgtcttcg gcctccctca cggtgaaccc gtccttgaac ggctcgagca tgcgggcttc
     3721 gaggacatcg gcggggatgc cgaggaaccg ggcgatcctg cccgcctccc tcctggtcag
     3781 gtacaccacg cccggctcac cggtgcagca cgcgccgcac ctcgtgcacc cgaacatgag
     3841 cccgtggtcg aagaaatagc tcctgccttc gagttcagcg aagggttcgc cgcccgtgtt
     3901 cgttcgcgcc ttccgcatgg tttcccataa tggttcacgg ggcgtcaaag gtcaaggtcg
     3961 gatgccgcgg cgcctcctgt cccgaccccc cggcgcaggc cgagtcacgc aaaggagaaa
     4021 ccggctcggg gaccgaccat ccctgccacg gtcttttccg aaaaaagtgc ttgccatttc
     4081 atcgtgattc ctataccacc aacccaggaa accaggaaag gagaggtgcg caaatggctt
     4141 acgttattac cgatgaatgc atatcatgtg gtacatgcgc cgaggaatgc ccggtcgacg
     4201 ccatcagcga gggcgaggac aagtatgtga tcgaccccga actctgcacc gactgcggca
     4261 cgtgcgccga ggcatgcccg gttgacgcca tcgaacccgg agagtgagtt caaccacacc
     4321 tcgaggcccg gcagagtggg cctgccgggt ctcccctcga caggacaccc cctgccttca
     4381 cacaccaggc cgtatccccc gttctctttc acgtacccct gcctgaaggg gcatgacagc
     4441 tgaccatcct cgaggacccc gcgcccgcac ccttgcgctc gatgccgccc cggtcccgcc
     4501 ttccccagag gccggcctcg ccctatccat tgttcctcgg tcgtcacccc gccttcgtcc
     4561 aggcggcgga cacctttggg gttcaaggcg ggaggtcttc tgcttcgatc ctcgagacca
     4621 gggtcaccgt gcagcgcacg gggcacggcc cctttcccat cccaggtatc aaggcctgag
     4681 tcgccgatcc actgggcccc gtccccgcct gtgcacccgg gcggtcgcct ccgccgcagc
     4741 gctcggcccc gtcttgggcc cgcaggctcg tttccacacc caaatgaatg tctcccctga
     4801 gtgcccttgt tgcccggcac cgtcttaggt ccgtaggctc gcttccaggc tccacggcac
     4861 gggcgggatc tcccatcctt gggtcaaccc gccatgggct tttctgtggg tcgccacccc
     4921 ggttcccact ggtgacgcga cctacacccg cccagatcac ggggcgaccc
//
LOCUS       DAOSCR010000127         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10699_length_4959_cov_3.386011, whole genome shotgun sequence.
ACCESSION   DAOSCR010000127 DAOSCR010000000
VERSION     DAOSCR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10699_length_4959_cov_3.386011"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            701..2260
                     /locus_tag="PLT69_04475"
     CDS             701..2260
                     /locus_tag="PLT69_04475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ13730.1"
                     /translation="MAEKRYIVSIDSGTQSIRAVLFDIEGNELAIEQRTFEPYTSLRP
                     GWAEQSTEDYWSKLCTVTGELMRKTDIDKSLIAGVGLTTQRSTVIPMDERGEALRPAI
                     IWLDQRTVDDPPPMSPLAKALFAVAGLSSAMEYARRHSIFLWIRLNEPEVYRKTHKFV
                     QASGFFVRKLTGEFHDSVGMVTGIWPFEYNRLRWHPLSLAYDPLGIEKRHCVKLFRPD
                     EALGRITAQASRETGLPEGLPLVVGAGDKQSELLGAGAIDPEIAEISFGTATAMHTIT
                     RKYIKDGKMRFFTWPSAVPGAWDIEMFIYRGFWMVTWFKQEFGMREAIEAERRNVAPE
                     VVFDEVVSRIPPGSMGLVLQPYWSPMVYNRSAKGSIVGFGDVHTRAHIYRAILEGIGF
                     ELRRLAGIVQKRIGVRFREIRVGGGGSKSDVAVQIASDMFGLPVYRMATSEISALGAA
                     IDTAVATGVYGSIEEAVRAMVKTGRKFTPDERNTLTYDRLFREVYLRLYEVLGPVNKT
                     IAKITGYPAEV"
     gene            2853..4079
                     /locus_tag="PLT69_04480"
     CDS             2853..4079
                     /locus_tag="PLT69_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ13731.1"
                     /translation="MRGSKRAVILIGVLACLGMPIDTPAATARLSWSPNTEPDLEGYR
                     VFVGTSSRAYTKTITIGRITSIDIADLSPGATYYFALKAFDFSGNESPYSDEVRLSVP
                     SSPAGSGGSSGGSGSSSGVIDEIAAALERLQRDLMTLFGLGPEVPLYGLGSFSSVASA
                     SAPSGAYPEGAPLIDQAGLYAAALTRAYPVRDVILEVDQAFDLSVLYPDGSFLFFPLS
                     DTYPEIEDGIVTPGEPGSFLYVAFDEALEIDHVLRISVASEICDICSYDPGLHAVLAD
                     MTTGAVVAFGTGATDEARPLALCRGGSFAPAPSAVATSDPASLTFDILPYGLELASPA
                     VVTIPWQEAAALVEIYDEDRGLWVPIEDTLVEGGLVSFSTQELGRFRVSAPPEETRGD
                     DGSWAGAETCFIEACR"
     gene            4568..>4959
                     /locus_tag="PLT69_04485"
     CDS             4568..>4959
                     /locus_tag="PLT69_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13732.1"
                     /translation="MHKAHSHLVLAVFMILCVSWSLALAQEPADNTGQDFTRPTGKLE
                     LGVVHMDMIADAWSESIVAKASLPCTLEGLWKASISAEQPYTWIKGATRKNPYGRRLK
                     GFSEVVLEGLVIAPPEGGLGVLTPGIGAV"
BASE COUNT          866 a         1645 c         1628 g          820 t
ORIGIN      
        1 cgtcgcgtga agacttccgg attttctggg gcagggacga ggaacgcatc cgtttctggc
       61 ggacggttgc cgatgcatcc agggcgctcg gggaggagtt cctcgagatg ctcgaaaacg
      121 gcaccctccc cgagaggatg gaacccctcg gccggatcgt gtccgcctga gacgggggtg
      181 aggcccgctg cggccgggcc acggttgaaa gacccgccgg cacggccacg ccagcctgcc
      241 gggcatgtct ttggcgttca gccaaaggcg caaggcccca agacccctca caactgcctc
      301 gcccgcctgc ggcgcagaag ggggccgacg gccgatgcac gaggaggctt tcggccccag
      361 gtcactgcgt ggttgtcgtg cccgctcgaa tacgggaaag gtagacgctc gtctctgtga
      421 tcggcgaacc ctgtatcgtg cccgcgcaca tacggatcag atgatcccgt ccctcttccc
      481 ggcggcaaac cgtccatcgt gcccatgcac atgcgggaca ggtccccgcg caagcctgcc
      541 tcgcccgcgg gccctcgggt cccggaacct gcccgccttg tgcggcaggc ccgtgtgcat
      601 ctgagggaga tcgaggttcc ccatcgagca tgcgcgtgct acaatgggcc gaaaccggca
      661 acgtctccga gccgtgtcat gagatcgtat gggaggggcc atggccgaaa agcgctacat
      721 cgtctccatc gactcgggta cccagagcat cagggcggtg ctcttcgaca tcgagggcaa
      781 cgagctcgcc atcgagcagc gcaccttcga gccctacacc tcgctcaggc ccgggtgggc
      841 cgaacagagc accgaggact actggtccaa gctctgcacg gtgacaggcg agctcatgag
      901 aaagaccgac atcgacaaga gcctgatcgc gggcgtcggc ctcaccaccc agcggagcac
      961 ggtcatcccc atggacgagc ggggcgaggc cttgaggccg gccatcatct ggctcgacca
     1021 gcggaccgtc gacgacccgc ctcccatgag cccgctcgcg aaggccctct ttgcggttgc
     1081 cgggctgagc tccgccatgg agtacgcccg acggcactcg atcttcctgt ggatcaggct
     1141 caacgagcct gaggtctaca ggaagaccca caagttcgtg caggcatccg gtttcttcgt
     1201 gcgcaagctc accggcgagt tccacgactc ggtgggcatg gtcaccggca tctggccctt
     1261 cgagtacaac aggctccgct ggcacccgct ctctctggcc tacgacccct tgggcatcga
     1321 gaagcgccac tgcgtgaagc tcttcaggcc cgacgaggcg ctcgggagga tcaccgccca
     1381 ggcctcccgg gagacgggcc tgcccgaggg gctgcccctg gtggtgggcg cgggcgacaa
     1441 gcagagcgag cttctgggcg cgggcgccat cgacccggag atcgccgaga tcagcttcgg
     1501 cacggccacc gccatgcaca ccatcacgag gaaatacatc aaggacggca agatgcggtt
     1561 cttcacgtgg ccgtccgcgg tccccggcgc ctgggacatc gagatgttca tctaccgggg
     1621 cttctggatg gtgacgtggt tcaagcagga gttcggcatg cgggaggcga tcgaggccga
     1681 gcgcaggaac gtggcccccg aggtggtctt cgacgaggtt gtgagccgca taccgcccgg
     1741 gtccatgggg ctcgttctcc agccgtactg gtcgcccatg gtctacaacc gctcggccaa
     1801 gggctccatc gtcgggttcg gggacgtgca cacccgcgcc cacatctaca gggccatcct
     1861 cgagggcatc ggcttcgagc tcaggcggct ggcaggcatc gtccagaaga ggataggcgt
     1921 gcgcttcagg gagatccgcg tgggcggtgg cgggtccaag agcgacgtgg cggtgcagat
     1981 cgcatccgac atgttcggtc tcccggtcta ccgcatggcc acgtccgaga tctccgccct
     2041 cggcgcggcc atcgacacgg cggtggccac gggcgtgtac ggctccatcg aggaggccgt
     2101 cagggccatg gtgaagaccg gcaggaaatt cacgcccgac gagaggaaca ccctcaccta
     2161 cgacaggctc tttcgagagg tgtacctcag gctctacgag gtgctcgggc ccgtgaacaa
     2221 gaccatcgcg aagatcacgg gctacccggc cgaggtctga gaaaccctcg gcacgacccg
     2281 gcgccggcag gccgtctcgg gcgggccgca ccacagggac cggacatcgc acgggccgcg
     2341 ggactccgac acgagcccca cacatacgcc gaacggctcg atcccgcctt gaaggccgca
     2401 aaccggccca cgggggtacc tcgaggatca gcggccgccc ggccggggcc ttaaggggcc
     2461 ttggcatgaa cctgtggcgg cagacagcct cgggcgggaa ccgcaagggc atcgctgaat
     2521 gccgggcgtg cctggaaccc aaaccaccag ggcgaccgcc ggtatcgaga aaacatcacc
     2581 tgccggccaa gacagggccg ctttccggta tttcgtcgag gagtcccgcc acccccggcc
     2641 gccctttttg cggcacccgc acgatgcccg catcctggga gaggcgcgga ccacgagcgt
     2701 gaatggaaag cggcggaaag gcaggcgtgc gcaagcggcc ttgccctttc acggccgcca
     2761 cacctggtgc atgcaccgtc aagggccttg actttcgcct gcctttgtcg gagataggtg
     2821 acacagagtg cagtcacctc gcaaggggtc gtatgagggg ttcgaagagg gcggtcatcc
     2881 tgatcggtgt gcttgcatgc ctaggtatgc ccatcgacac ccctgccgcc accgcccgcc
     2941 tgagctggag cccgaacacc gagccggacc tcgaggggta ccgcgtgttc gtcggcacgt
     3001 ccagccgggc gtacacgaag accatcacca tcggccgcat cacctccatc gacatcgccg
     3061 acctctcacc gggcgcgacc tactacttcg ccctcaaggc gttcgacttc tcggggaacg
     3121 agagcccgta ctcggacgag gtgcgtctca gcgtcccgtc atcccctgcg ggctcgggcg
     3181 ggagttcggg cggttccggt tcttcctcgg gcgtcatcga cgagatcgca gccgctctcg
     3241 agcgtctgca gcgcgatctc atgaccctgt tcgggctcgg ccccgaggtg ccgttgtacg
     3301 gcctggggag cttcagctcg gtggcatcgg catccgcacc ttcaggggcg tatcccgagg
     3361 gagcgccgct gatcgaccag gcgggactgt acgcggctgc cctcaccagg gcgtatcccg
     3421 tccgggacgt gatcctcgag gtggaccagg cattcgacct cagcgtgctg tatccggacg
     3481 ggagcttcct cttcttcccg ctcagcgaca cgtaccctga gatagaggac gggatcgtga
     3541 cgcctggaga gccgggatcg tttttgtacg tcgccttcga cgaggcgctc gagatcgacc
     3601 acgtcctgag gatctcggtt gcatccgaga tctgcgacat ctgttcgtat gatccgggcc
     3661 tgcatgcggt ccttgcggac atgacgacag gggcggtcgt cgccttcggg accggcgcca
     3721 cggacgaggc caggcctctg gccctctgca ggggcggatc ctttgcgccc gcgccgtccg
     3781 cggtcgcgac ctcagaccct gccagtctca ccttcgacat cctgccctac ggcctcgagc
     3841 tggcatcacc ggcggtggtg acgatcccct ggcaggaagc ggcggccttg gtggagatct
     3901 acgacgagga tcgaggcctg tgggtcccca tcgaggatac cctcgtggaa ggaggtctcg
     3961 tgagcttctc cacccaggaa ctcggacggt tccgtgtgag cgccccaccc gaggagaccc
     4021 ggggggacga cggttcctgg gcaggggccg agacgtgctt cattgaggca tgccgctagc
     4081 ttgagcgtcc tcaccgccac ccgtctcgcg taacgtgcgg cggtgtcgac caccccgtga
     4141 tgtgccggcc cttcccgtgt gtgccgccgt tgcgtgcggg gcggcacgat agggcgtaaa
     4201 ttgcttgtcc ttcagcatat acaggaagag cgtgaccttg ggcgagattc ggccttgata
     4261 tcatccctga gggctctctc gtgacgcacc cggacaaaca cgagacgccc ggtctcaaga
     4321 agcggcatgc agcggcggtg ccccgggcag cggccgcagc gctcggcctc aagggtcagt
     4381 tcgcgtctcg ggagatgcgg ccggtaggcg catgcaggag atggcgaaag agggtttcat
     4441 gctcacaggt ggaggaatgc cttttatggc ctgctttcgt tgccctcgca tcaaaacctt
     4501 gctattctga catacaggcc atcgagcctc gcatgccatt ccaacgctga gagaggagga
     4561 tcaccccatg cacaaggccc actcgcacct cgttctcgct gtcttcatga tcctttgcgt
     4621 atcctggagc ctcgcgctcg cgcaggaacc ggcggacaac actggccagg acttcacgcg
     4681 gcccacgggc aagctcgagc tcggggtggt gcacatggac atgatcgccg atgcatggag
     4741 cgagtccatc gtggcaaagg cgagcctgcc ctgcacccta gagggcctct ggaaggcctc
     4801 catcagcgcg gagcagccct atacctggat caagggcgcg accaggaaga acccttacgg
     4861 aaggaggctc aagggcttct cggaggtcgt cctcgaaggg ctcgtgatcg ccccgcccga
     4921 gggcgggctc ggggtgttga ccccgggcat cggcgccgt
//
LOCUS       DAOSCR010000128         4887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10892_length_4887_cov_3.677359, whole genome shotgun sequence.
ACCESSION   DAOSCR010000128 DAOSCR010000000
VERSION     DAOSCR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10892_length_4887_cov_3.677359"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLT69_04490"
     CDS             <1..1103
                     /locus_tag="PLT69_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOJ13733.1"
                     /translation="FGGTLVDKKGPRFVATIGAILFGIATILSGLADQVASLPLLYLS
                     YGIIGGLGNGFCYVTPIATLIRWFPDKRGLVTGLAVMGFGLGAFFMGKIAPAMINAYA
                     GGLTESGVALTFYIWGVIFLVLCVGAAQLFKNPPAGWLPAGFTPSATTVSAADSYTFD
                     EAIKTPQWWMLWGMLFLNVSAGLGLISQLSPLAQDYYKPLVSDPNILNDPEALKKTLA
                     VAGGTVVSISAIFNGLGRLFWARVSDIKAIGRRGVFGFMFASQAVIYILVATGVISQY
                     YIFMIAACYLLACYGGGFATMPAFAADNFGPGYIGKVYGFMLTAWACAGIVGPLAFAQ
                     FKDQSLYIAAALLIIGFVLVLAFRRREHHKKA"
     gene            complement(1237..>1610)
                     /locus_tag="PLT69_04495"
     CDS             complement(1237..>1610)
                     /locus_tag="PLT69_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13734.1"
                     /translation="IAIEPSMVMAVNSRIPTLDMTGTAGAVVDGSGAIRIFFATRGPN
                     SSSRQKGIYMATSSDGTTFTVSYPPVIGEFSIGDDFYAPMDAEAVFTDEGLRLYYWIG
                     TSEVLPESGIYSLFNPSITYD"
     assembly_gap    1611..1620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1621..2241)
                     /locus_tag="PLT69_04500"
     CDS             complement(<1621..2241)
                     /locus_tag="PLT69_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13735.1"
                     /translation="MEMKNDRKLLQVSSILAVLFILALSIFSCGGGSGGSGSEDSISL
                     ANGWEREDGVRFTDPYAFNPDIVSLEEGGFRMYFEKEVSQGEHNIYSAFSEDGLIWTQ
                     EDGTRVAGANMPAAVALPDGRVRIYYNWGGVVKSSISEDGLVFAAESGTRLSPDEDDE
                     TGGIKHPDVIRLPDGTFRMYYDAVDSEEVYRIKTAVSDDGLSWTKQG"
     gene            complement(2447..3646)
                     /locus_tag="PLT69_04505"
     CDS             complement(2447..3646)
                     /locus_tag="PLT69_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ13736.1"
                     /translation="MTIPFSIAALWLGLAVIATVIASYLRISMALVEICVGMAAGAAC
                     EYYLGQGLIGADAEWLRFLASSGAILLTFLAGAELEPQVMRAKWKEVTVVGIVGFLAP
                     FAGCAALAHYLLGWSPQASLLAGVALSTTSMAVVYAVMLETGFNRTEFGKGILGACFV
                     NDLGTVIALGLLFSPFTAKTLVFIAASAVFLAVLPRLSKRFTRKFGHRTAAIRAKWVM
                     FTLFGLGALALWSGSEAVLPAYVAGIVLAEFAAKDELWIRRMRTLCIGFLTPFYFLRA
                     GSLVSLPAIAAMPLVFLALLAGKVASKIFGLYPFISMFRKETNERWYYTLMMSTGLTF
                     GTISALYGLSHGIVGREQYSFLVAGVIASAVIPTMIANLAFIPYSLLPGTPEPTAEEI
                     EEGPGNE"
     gene            complement(3715..>4887)
                     /locus_tag="PLT69_04510"
     CDS             complement(3715..>4887)
                     /locus_tag="PLT69_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13737.1"
                     /translation="YLAGRSPLRGVVLIMDSRHPLTDLDVDLIEWFAPTGKPIHILLT
                     KADLLTPEQQREVVEFFRKTLKHKLDREFPVYPYSTRTGTDQLRQRIASDLLAGISAN
                     LDHEFTRIVRFKTQSLLGRCLTYLEVARASSLEADQDREALRDKVLGEKMAMERIREE
                     VSVITREIQGQTRMLLKNHLDRFEAPLTEKIRTRLSHELPTWKGNLWKLTRRYEEWLA
                     DTMTEEIGRISQAENVHFFGTLNKAHRTLMRYLETFRLVLGESISRVLGIRLPDADWK
                     IEVAEPEHPDIRVLFAFDIHWDLLWFLIPMFVFRPLFERHYLREVAWAVEMNLSRLAA
                     QWESRINKAIEVMKDQALAYIEEELATIDALLSDTKGRTKELDDLIGMLRSMQNGS"
BASE COUNT          924 a         1510 c         1459 g          984 t
ORIGIN      
        1 ccttcggagg aacgctcgtc gacaagaagg gcccgcgttt cgtcgccacc atcggcgcca
       61 tactcttcgg tatcgccacg atcctctcgg gtctcgcaga ccaggtggcg agccttcccc
      121 tcttgtacct gtcctacggg atcatcggcg gtctcggcaa cggcttctgc tacgtcacgc
      181 ccatcgcaac gctgatccgg tggttccctg acaagcgggg cctggtgacc gggctcgcgg
      241 tcatgggctt cggcctgggc gcgttcttca tgggcaagat cgcacccgcc atgatcaacg
      301 cctatgctgg cgggctgacc gagtcaggcg tggccctgac cttctacatc tggggcgtga
      361 tcttcctggt gctgtgcgtc ggcgccgccc agctcttcaa gaacccgccc gcgggctggt
      421 tgccggccgg gttcaccccc tcggcaacca cggtttccgc cgccgactcc tacaccttcg
      481 acgaggccat caagacaccc cagtggtgga tgctctgggg catgctgttc ctgaacgtgt
      541 ctgcgggcct cggtctcatc tcccagcttt cgcccctcgc gcaggactac tacaagcctc
      601 tcgtttctga cccgaacatc ctgaacgacc ccgaagcgct caagaagact ctcgctgtcg
      661 ccggaggaac ggtagtgtcg atctctgcga tcttcaacgg gctgggcagg ctcttctggg
      721 cccgtgtgtc agatatcaag gccatcggca gaaggggcgt gttcgggttc atgttcgcgt
      781 cccaggcggt gatctacatt ctcgtggcaa caggcgtgat cagccagtac tacatcttca
      841 tgatcgccgc gtgctacctg ctcgcctgct acggcggcgg cttcgccacc atgcccgcct
      901 tcgcggccga caacttcggg ccgggctaca tcggcaaggt ctacggcttc atgctcacgg
      961 cgtgggcttg cgcaggtatc gtgggcccgc tggccttcgc ccagttcaag gaccagtcgc
     1021 tctatatcgc ggccgcactg ctcatcatcg gcttcgtgct cgtcctcgcg ttcaggcggc
     1081 gggagcatca caagaaggct tgatctcaaa acaaacgcat tgcttgagaa gcgagggggg
     1141 cggaccgtga ctgcccccct cgcttcaatc gttcttccac caggcgatcc tcgacgtctc
     1201 tatgcacgtg gcctggagac aaccctctga ccaacttcag tcgtacgtga tggacgggtt
     1261 gaagaggctg tatatgcctg actccggcag aacctcagag gttccgatcc agtagtacaa
     1321 cctgagcccc tcatcggtga agacagcctc ggcgtccatg ggggcgtaga aatcatcccc
     1381 gatggaaaac tctccgatga caggcgggta agatacggta aaggttgtgc cgtctgagga
     1441 tgtcgccatg tagatgcctt tctggcgcga agaagagttc ggccctctcg tggcgaagaa
     1501 gatacgtatg gcgccggatc cgtcaaccac cgcacccgct gtcccggtca tgtccaacgt
     1561 ggggattctg ctgtttacgg ccattaccat ggatggctct atcgcgatcc nnnnnnnnnn
     1621 cccctgtttg gtccatgaaa ggccatcgtc cgatacggcg gtcttgatcc tgtacacttc
     1681 ttcactgtcc accgcatcat agtacatccg gaaggtcccg tcgggaagcc tgatcacgtc
     1741 ggggtgtttt atccctccgg tttcatcgtc ttcgtccggg ctcagacgcg taccagactc
     1801 ggctgcaaag acaagcccgt cttcagatat ggagctcttc acaaccccac cccagttgta
     1861 gtatatgcga accctcccgt cgggaagcgc tacggctgcg ggcatattgg ctcccgccac
     1921 ccgggttccg tcctcttgag tccaaatcag tccgtcttcg gagaaagcgc tgtagatatt
     1981 atgctctcct tgggagactt ccttctcgaa atacattcgg aatccgcctt cctccagaga
     2041 aacgatgtcc gggttgaatg cataaggatc cgtgaaacgc accccatcct ctctctccca
     2101 gccatttgcc agagaaatgg aatcttcgga tccagacccc cccgaaccac cgccgcacga
     2161 gaagatggat agggccaaga tgaacagtac ggccagaatc gagcttacct gcaagagctt
     2221 gcgatcgttc ttcatttcca tggatctcct ttataagtga ttaagatagt cttcggaaaa
     2281 tgcaccgctc aacttgaaga gatgtgaccc acgcttcgac gctgattgat cacaagactc
     2341 gagttcattt gctgtaagaa caggagctga aaggcgacag acctcgtgcg cgggtttgtg
     2401 agaaccccct gggcgacctt tcgccgtctt aggttcagag gcaccgtcac tcgttaccgg
     2461 gaccttcttc gatctcctcg gcagtaggtt caggcgttcc cggcaacagc gagtacggga
     2521 tgaaggcgag gttcgcgatc attgtgggga tgaccgcgct cgcgatcacg ccggccacga
     2581 gaaaggaata ctgctcgcgg ccgacgatgc catgcgacag cccgtacaag gcggagatgg
     2641 tcccgaaggt caggcccgtg gacatcatga gggtgtagta ccaccgctcg ttcgtctcct
     2701 tcctgaacat ggatatgaac gggtagaggc cgaagatctt gctcgccacc ttgccggcca
     2761 ggagcgccag gaagacgagc ggcattgcgg ctatggcggg caggctcacc agggatcccg
     2821 ccctgaggaa gtagaagggt gtcaggaacc ctatgcacag ggtccgcatc cgtctgatcc
     2881 acagctcgtc cttcgccgcg aactcggcaa gcacgatgcc cgccacgtag gcgggcagca
     2941 ccgcctcgct gcccgaccac agggccaggg cgcccaggcc gaacagggtg aacatgaccc
     3001 acttggcccg gatggcggct gtccggtgac cgaatttcct ggtgaagcgc tttgagagcc
     3061 ggggaaggac ggccaggaaa acggcgctcg ccgcgatgaa gacgagggtc ttggcggtga
     3121 agggcgaaaa gagcagccca agagcgatga ccgtacccag gtcgttcacg aagcatgcac
     3181 cgaggatgcc cttgccgaac tcggtgcggt tgaaaccggt ctccagcatc acggcataga
     3241 cgaccgccat ggaggtggtg gagagcgcca cccctgccag caggctcgcc tggggagacc
     3301 accccaagag atagtgcgcc agggccgcgc acccggcaaa gggcgccagg aagcctacga
     3361 tgcccaccac ggttacttct ttccacttgg cgcgcatcac ctggggttcc agttcggcgc
     3421 cggccaggaa ggtgagcagg atcgcgcctg atgacgccag gaagcgcagc cattcggcat
     3481 cggcgccgat gagcccctgt ccgaggtaat actcgcacgc cgcgcccgcg gccatgccca
     3541 cgcagatctc cacgagggcc atggagatcc tcaggtagga ggcgatgacg gtcgcgatga
     3601 ccgcgagccc gagccacagg gcggcgatgg agaagggtat ggtcatgggt atctcctttg
     3661 tggttttcgt gaatcccgcc aatcaggaat ctgcgtggaa aacgcgtggg ttggtcacga
     3721 cccgttctgc atggacctga gcatgccgat caggtcgtcc agttccttgg tcctgccctt
     3781 ggtgtcggac agcagggcgt cgatggtggc gagttcctct tcgatatagg cgagcgcctg
     3841 gtccttcatg acctcgatgg ccttgttgat ccgcgactcc cactgggcgg cgagccgcga
     3901 caggttcatc tccacggccc aggcgacctc gcgcaggtag tgccgttcga agagcggcct
     3961 gaacacgaac atggggatga gaaaccagag aagatcccag tggatgtcga atgcgaacag
     4021 cacccggatg tccgggtgtt cgggctcggc cacctcgatc ttccagtctg catccggcag
     4081 cctgatgccc agaacgcggg agatgctctc tcccaaaacc agacggaacg tctcgaggta
     4141 gcgcatgagc gtccggtgcg ccttgttcag ggtgccgaag aagtggacgt tctcggcctg
     4201 cgatatccgt cctatctctt cggtcatggt gtcggcgagc cactcctcgt aacgcctcgt
     4261 gagtttccac aggttgcctt tccaggtcgg cagctcatga gagagcctcg tcctgatctt
     4321 ctccgtaagg ggtgcctcga acctgtccag gtggttcttc aggagcatgc gggtctgtcc
     4381 ctggatctcc cgggtgatga cggacacctc ctccctgatc cgttccatgg ccatcttctc
     4441 gcccaggacc ttgtccctca gtgcctctcg gtcctggtcc gcctccaggg aactcgcccg
     4501 tgccacctcg aggtaggtca ggcacctgcc gagcagggac tgggtcttga agcggacgat
     4561 gcgggtgaac tcatggtcga ggttggcgga gatgcccgcg aggagatcgg atgctatcct
     4621 ctgcctgagc tggtccgtgc cggttcgtgt cgagtagggg tataccggga attcccggtc
     4681 gagcttgtgc ttcagggtct tccggaagaa ctccaccacc tctctctgct gctcaggggt
     4741 gagcaggtcg gccttggtga gcaggatgtg gatcggcttg ccggtcggcg cgaaccattc
     4801 gatcagatcg acgtcgagat cagtcaacgg atgacggctg tccatgatca gcacgacgcc
     4861 cctgagcggc gagcgccccg ccagata
//
LOCUS       DAOSCR010000129         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10900_length_4883_cov_3.130903, whole genome shotgun sequence.
ACCESSION   DAOSCR010000129 DAOSCR010000000
VERSION     DAOSCR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10900_length_4883_cov_3.130903"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLT69_04515"
     CDS             complement(<1..378)
                     /locus_tag="PLT69_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13738.1"
                     /translation="MMDFPLLIRTFLLRSAKYFPKKEIVSVFPEGVHRYTYADYYKRT
                     CQLAHALKSLGIRRGDRVASVALNDHRHLELYFGVPCYGAVLHTANFRLPPNHLTYII
                     NHAEDRVIFVDEDLLMFVELVRDK"
     gene            complement(1060..2307)
                     /locus_tag="PLT69_04520"
     CDS             complement(1060..2307)
                     /locus_tag="PLT69_04520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13739.1"
                     /translation="MFDYIMTEDQKRLRDEARAFTRWVPRQLILDMDAEKVQFPREYL
                     QEAGRRNLLGIRISRELGGRGLGWIEDGIVAEEVGVASYSLACLWGVGADIVCEAVEL
                     FGCDELKQAVVVPLLKGETFAAEGLTEPRGGSDFFGATTYAKKDGSDWIINGQKRFIV
                     GAEGADWFLVYAVTDPDAPPHRRMTAFMVPRTEGVETKYIYGLMGVRGGGAGRLVLKD
                     VRVPERYALNGINGGFDVFVRMMIPERLGTAAMTLGSVRPAIEIATRYTSRRKAFGLP
                     IQSFQAVGFKVADSVMLLDAARSMVYSTCLAVDSRRAHPNRVRRLVSETKKFVTEAAW
                     TIANNCMQVVGGIGYTNVYPLERIVRDIRLSMIWVGTNEIMQLIVQNEWYKEYLKVIS
                     KEDVRDVEADAPSADAEEEKVYE"
     gene            2647..2997
                     /locus_tag="PLT69_04525"
     CDS             2647..2997
                     /locus_tag="PLT69_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ13740.1"
                     /translation="MGFKEIGRKIQLAREEKGLTQAELAQELGMTQAGLSNYELGKRR
                     LYLNQIERIARVLGKDIAFFIGGANHESAASDESSHLMKTVAERLRNLDEDDLRSIND
                     YLDFLAWRKRRDRH"
     gene            2984..4108
                     /locus_tag="PLT69_04530"
     CDS             2984..4108
                     /locus_tag="PLT69_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13741.1"
                     /translation="MIDTEACPHGLMEEIGTLAREVRDVLDAGLPARQRAAGIHSAFA
                     KRGYHALVVPRESGGRGLDHLTAGLIYEALSYELPGTLHGTMTTAHCALMISSGLTNE
                     FHLERLEAIAGEDHPGAFCLTERDAGSDIGAVGTRAETIPGGFTITGEKAIAINHAIA
                     KTLVVFATLPPASGRAALNAFVVDPSAEGIRQGPALDTPGMAGGILGPISFDRARVPH
                     ECLLGERGSGYLLFMETLDKGRPLVAACCTGEADRALDCAIDHARKRYQFGKPLYAFQ
                     DVSFRIAELSTRLKAARLLWKDALERIDRGRPFTMEASMAKLFASSTLMDIASFALEL
                     TGYRSVGRNTELERIYHGARLMKSIDGTEAVQKMVIASQL"
     gene            4117..>4883
                     /locus_tag="PLT69_04535"
     CDS             4117..>4883
                     /locus_tag="PLT69_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ13742.1"
                     /translation="MARHATIGYASVIVAALLWASCGTAGKALFGSGIPPALLVQTRV
                     TLASLILGAFLAAWRPSLLRIGVKDLGFFLVFGGVFMASMQLAYFMAISKIQVAAAIL
                     VQYLAPVIVAAYSMAFWHERVTAKKAAALALSMAGCYLVVGGYSLRLVSLNLPGILWA
                     LLSAFAFAGTTLLGEKGMHTHNPWTSLMYALLFSALSLNVAYEPLQILSVSYTPIQWG
                     AILYIVVFGTLIPFGLYLTGVNYIRSTRTIITATLEP"
BASE COUNT          858 a         1630 c         1530 g          865 t
ORIGIN      
        1 cttgtctctc acgagctcga cgaacatgag gagatcctcg tccacgaaaa tgaccctgtc
       61 ctcggcgtgg ttgatgatgt aggtcaggtg gttcgggggg agcctgaagt tggcggtgtg
      121 gagcacggcg ccgtagcagg gcacgccgaa gtatagctcc aggtgccggt ggtcgttgag
      181 cgccacgctc gcaacgcggt cgccccgccg tatgccgagc gatttcaggg cgtgagcgag
      241 ctggcacgtc cttttgtagt agtcggcata ggtgtacctg tgcacgccct cgggaaacac
      301 cgacacaatc tctttcttgg ggaagtactt cgccgaccgc aacaggaagg tcctgatcaa
      361 gaggggaaag tccatcatgg cgcctgtctc cttttttgtt tgtatgtatg tattacaata
      421 ctattataag cacattggcg cggcgtgcac aagcccgttt ttgctgttaa cgggaaacgg
      481 cgaaccaagg gtcaggtcag cctctcggct gggttgtcgc cgggtctcga ccctcgagcg
      541 aggccacagg gccggctccg gccaccctcg ccggcatgac gccctctcgc gcggccgggg
      601 gcagggagag cgcggcgaaa gaggagggat caccccggcg ggtggaacga gggccttcgg
      661 accctccacc cgacatgacc ggaccggcgg caagggcgcg ccttgggcct tccctcacgg
      721 gcgtccgccc gcggatgcga gagggggtgc gccgggggac gggaaggaac ttcctcggat
      781 tttccaggat agatccgggc gagcggggcc ttcgtcgcct tcacccagca ggcccgggca
      841 tgacgcggcc gtccgaggcc tgcagaaaaa gccgcaagcc cggcatcagg aacattccac
      901 ggcgtcgagg gacgtgacgc cgggcgcccg ggatcatgaa cgtaaaggag gaatgggcat
      961 atccgggcgc ccggcgcctg ggctgagaac cgaggatgcg aggtctttcg gtcttcggtg
     1021 cacgcacccg atacgaggtg cggccccgct cgtcaccgcc tactcgtaga ccttctcttc
     1081 ctctgcgtcg gcgctcggcg catccgcctc cacgtcacgc acgtcttcct tcgagatcac
     1141 cttgaggtac tccttgtacc actcgttctg cacgatgagc tgcatgatct cgttcgtccc
     1201 gacccagatc atggagagcc tgatgtcgcg gacgatcctc tccagcgggt agacgttcgt
     1261 gtaaccgatg ccgcccacca cctgcatgca gttgttcgcg atcgtccatg ccgcctcggt
     1321 cacgaacttc ttggtctccg agaccaggcg cctgaccctg ttggggtgcg cccgtcggga
     1381 gtcgaccgcg aggcacgtgg agtagaccat ggagcgggcg gcgtccaaga gcatgaccga
     1441 gtccgccacc ttgaagccca cagcctggaa cgactggatg gggaggccga aggccttgcg
     1501 cctggaggtg tagcgcgtgg ctatctcgat ggcgggccgg accgacccca gcgtcatggc
     1561 cgccgtgccg agcctctcgg ggatcatcat gcgcacgaac acgtcgaacc cgccgttgat
     1621 gccgttgagg gcgtatctct cgggcacccg gacgtctttg agcaccagcc tcccggctcc
     1681 gccgccgcgc acgcccatga gtccgtagat gtatttcgtc tcgacaccct cggtcctcgg
     1741 caccatgaag gcggtcatgc gcctgtgggg cggggcgtcc gggtcggtga cggcgtatac
     1801 gagaaaccag tccgctccct cggcgccgac gatgaaccgc ttctggccgt ttatgatcca
     1861 gtcggaacca tcctttttcg cgtacgtggt cgccccgaag aaatcggacc cgcccctcgg
     1921 ctcggtgagg ccctccgccg cgaaggtctc ccccttcagg agcgggacca cgacggcctg
     1981 cttgagctcg tcgcatccga agagctccac ggcctcgcac acgatatccg ctcccacccc
     2041 ccacaggcag gcgagcgaat agctggcgac gcctacctct tccgcgacga tcccgtcctc
     2101 gatccacccg aggcccctgc ccccgagctc gcgggagatc cttatcccca gaaggttcct
     2161 ccttccggcc tcctggaggt attccctggg aaactgcacc ttctccgcat ccatgtccag
     2221 gatgagctgc cggggcaccc acctggtgaa cgcccgcgcc tcgtccctga gcctcttctg
     2281 gtcttcggtc atgatgtagt cgaacatggt ctctgcccgt cccttataac gttttgtttt
     2341 atcattatcg tcatctgtta tgaccgtgtc aagcccccga ggcaggaagg cgtagcggcc
     2401 tcatgggtgg cttcccgcac cgggcaagca tggggacgtc ctgttcacgg tctttcccca
     2461 tggggactgg cggccggatg gaaggcattg acaccccttt cccctccccc gagacgaaga
     2521 cccacccgtg gccacgtcca cggcgatggg tcttcacgag aaaggcgcgg ggctcacggg
     2581 atccctcacg ccgcggtcaa aacggccttg catttttccc ggtaataccc aacataaaag
     2641 accggcatgg gattcaagga gatcgggaga aaaatccagc ttgcaaggga ggaaaagggc
     2701 ctcacccagg ccgagctcgc acaggagctg ggcatgaccc aggccggcct gtcgaactac
     2761 gagctgggca agcgcaggct ctacctcaac cagatagaac gcatagcccg cgtcctgggc
     2821 aaggacatcg ccttcttcat cggcggggcg aaccacgaga gcgcggccag tgacgaatct
     2881 tcgcacctga tgaagaccgt ggccgagcga ctgaggaacc tggacgagga tgacctgagg
     2941 tcgatcaacg actatctcga ctttctcgcg tggaggaaac gccgtgatag acactgaagc
     3001 gtgcccccat gggctcatgg aggagatagg gactctcgcc cgagaggtcc gcgatgtcct
     3061 cgacgcagga ctcccggcac gtcagcgggc ggcgggcata cactcggcct tcgcgaaaag
     3121 gggctaccac gcgctcgtcg tgccgaggga atcgggaggc cgggggctcg accacctcac
     3181 ggcgggcctg atctacgagg cactgagtta cgaactgccg ggtacccttc acggcacgat
     3241 gaccacggcc cactgcgcgc tcatgatctc gagcggcctc accaacgagt tccacctgga
     3301 gcggctggag gcgatcgccg gtgaggatca cccgggggcc ttctgcctca ccgagcgcga
     3361 cgccggctcc gacataggag cggtgggcac gcgggcggaa acgatccccg gcggcttcac
     3421 gatcacgggc gagaaggcta tcgccataaa ccacgccata gccaagaccc tggtcgtctt
     3481 cgccacactc ccccccgcat ccggcagggc cgcgctcaac gccttcgtgg tggacccgtc
     3541 ggccgagggc atccgccagg ggccggccct cgacaccccc ggcatggccg gcggcatcct
     3601 cgggccgatc tccttcgacc gcgcgagggt acctcacgaa tgcctcctcg gcgagagggg
     3661 cagcgggtac ctcctcttca tggagacgct cgacaagggc aggcccctgg tggcggcctg
     3721 ctgcacgggg gaagcggaca gggcgctcga ctgcgcgatc gaccacgcac ggaaacgata
     3781 ccagttcggc aaacccctgt atgccttcca ggatgtgtcc ttccggatcg cggagctgtc
     3841 cacgaggctc aaggcggcga ggctgctctg gaaggacgcc ctcgagcgga tcgaccgggg
     3901 acgccccttc accatggagg cgtccatggc gaagctcttc gcatccagca ccctgatgga
     3961 cattgcatcg ttcgccctcg agctcaccgg gtaccgctcg gtgggcagga acaccgagct
     4021 cgagcggatc tatcacggcg cacggctcat gaaatccatc gacggcacgg aggcggtgca
     4081 gaagatggtg atcgcctcgc agctgtgagc ggccccatgg cgagacacgc aacgataggg
     4141 tatgcgagcg tgatcgtcgc cgccctgctc tgggcctcct gcggaacggc cgggaaggcc
     4201 ctgttcggct cgggcatccc tcccgccctg ctggtgcaga cgcgtgtcac cctcgcctcc
     4261 ttgatactcg gggcgttcct tgccgcatgg cggccctcgc tgctcaggat aggcgtgaag
     4321 gaccttgggt tcttcctcgt gttcggcggg gtgttcatgg cgagcatgca gctcgcgtac
     4381 ttcatggcca taagcaagat ccaggtggcc gcggccatcc tggtgcagta cctggccccc
     4441 gtgatcgtcg ccgcgtactc catggccttc tggcacgagc gggtgacggc gaagaaggcg
     4501 gcggccctcg cgttgtcgat ggccggctgt tacctggtgg tcgggggcta cagcctcagg
     4561 ctcgtgagcc tcaacctgcc gggcatcctc tgggccctcc tgtccgcctt cgccttcgcg
     4621 ggaacgacgc tgctcgggga gaagggcatg cacacccaca acccctggac gtccctgatg
     4681 tacgccctcc tgttctccgc actcagcctg aacgtcgcct acgagcccct gcagatcctg
     4741 tccgtctcgt acacccccat ccaatggggc gcgatcctct acatcgtggt gttcgggacg
     4801 ctcatcccct tcggcctcta cctcacgggg gtgaactaca tacggtccac ccgcaccatc
     4861 atcacggcca ccctcgaacc cat
//
LOCUS       DAOSCR010000130         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10915_length_4877_cov_3.990460, whole genome shotgun sequence.
ACCESSION   DAOSCR010000130 DAOSCR010000000
VERSION     DAOSCR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10915_length_4877_cov_3.990460"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..785
                     /gene="lptB"
                     /locus_tag="PLT69_04540"
     CDS             57..785
                     /gene="lptB"
                     /locus_tag="PLT69_04540"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOJ13743.1"
                     /translation="MEEVILRAQGLEKSYGKHKAVNGLSLRCAPGEVIGLLGPNGAGK
                     TTTFYMIVGLVRPDSGAVYLGDEDITEFDLAGRALRGIGYLPQEPSVFRGLSVEDNIA
                     VPLEARGLKGADLKGRVDEIVGAFGLDHVRTSKGVSLSGGERRRVEIARAMALDPKYM
                     LLDEPFAGIDPVTVKDIQEMIITLKNSSIGVIITDHNVKETLKICDRAYIIHQGVVIE
                     EGTPAQVVANEDVRRYYVGRDFSL"
     gene            803..2182
                     /gene="rpoN"
                     /locus_tag="PLT69_04545"
     CDS             803..2182
                     /gene="rpoN"
                     /locus_tag="PLT69_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOJ13744.1"
                     /translation="MALELKQHLKLAQQLIMTPQLQQAIKLLQLSRLELQEYIETELQ
                     QNPLLEVDTTRVKEDTLEEIAQRKELRQRWEAYLETYGQDNIPYYEDEERPSFEATTS
                     KPEGLVDHLMWQLRLNDFTDEERAIGLFIIGNLDHNGYLPMDIPSICQETGASEEVVE
                     RVLSRVQQFDPQGIAARDLKDCLKIQARQLGLENSLVWKIIENHLQDLQTKNYHKIAQ
                     ELSTSVEEVAQAAGIITNMEPRPARDYSDDPPQYIVPDVYVVKLEGEYVVLLNEEDLP
                     VLKFSREYQEMMKSNSMSKLARDYLSECYKSAQWLMKSIQQRQNTLVRVTESIVKFQR
                     EFLDHGISHLKPLVLRDVADDVGMHESTISRVTSNKYVHTCQGTFELKFFFNSGISKS
                     DGSFVASQSVKNEIEGIIKAEDPRHPLSDQAIALILKQKGVSIARRTVAKYRELLGIL
                     PSNQRKKHF"
     gene            2207..2749
                     /gene="raiA"
                     /locus_tag="PLT69_04550"
     CDS             2207..2749
                     /gene="raiA"
                     /locus_tag="PLT69_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ13745.1"
                     /translation="MYMLTNITFKNIESSDALRDYALKRLSKIDKYIDRTAEAHVVFS
                     VEKRRHKADITLSADGTVINAVEITDDLYAAIDMVMDKLERQLKKHKGKLQDKKAPAK
                     TGVPASLSAEEAPKPRKKPPRIAKGTAYTVRPMSLDEAIEEMLAGKEDFVLFRNTKSQ
                     LLNLLYRRADGELAIVEPRL"
     gene            2746..3210
                     /locus_tag="PLT69_04555"
     CDS             2746..3210
                     /locus_tag="PLT69_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ13746.1"
                     /translation="MKIADILSPECVISDLRGATKKEILEELALPVSRIHRINLDEIV
                     SILMAREQLGSTGMGDGVAIPHGKVPGLKDIVGALGKSAKGLDFDSIDKKPCHIFFML
                     LAPSTFVTQHLKALARVATLLKDPSLRHRIMESSSASEIHHLLIDHDSTIED"
     gene            complement(3307..4467)
                     /locus_tag="PLT69_04560"
     CDS             complement(3307..4467)
                     /locus_tag="PLT69_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ13747.1"
                     /translation="MAVLDQKAQQELRVRAADVAQNVADFLRERQKDVLIATILPADA
                     QAYKKFLDTKTFELWVKKDTGIAREQLPLYVELSLTDSAGREIMKIKDGKVVGADGLL
                     DISNPANTTYKSEDYFIKARELDKGEVYVSHVTGWYVTREEFEKGKRFEGIIRLATPV
                     FDKRGFTGVLTLTLDARALSRFTDTVVPTEAGHVVESDAATGNYAYLVDNRGFVISHP
                     ADYHIVGLYKDGTQVPPITKGTWDEKMKKGEEVLNVNELGGVDQTLPEVAKDAAQGLS
                     GVKTYQFEGHTKVVAYAPVPFYTRDYPKPAGFGWVAMAVDVEKFLEQAKEASGKVEKE
                     AQAWLTTIVLIIFGAAVILFTIAVILSRGINRSIASEVPPEALKPKGYDDED"
     gene            complement(4663..4875)
                     /gene="atpE"
                     /locus_tag="PLT69_04565"
     CDS             complement(4663..4875)
                     /gene="atpE"
                     /locus_tag="PLT69_04565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOJ13748.1"
                     /translation="MGLGCIGAGAGIGNAAAGASEAVGRNPAAQGKIMMTMMVGMAMA
                     ESIAIYALVITLILIFANPYKTFLFG"
BASE COUNT         1071 a         1489 c         1483 g          834 t
ORIGIN      
        1 ggagaccaga gggtgagggt acgggtggag gcccaggagg gctcggggat cctgcaatgg
       61 aagaagtgat cctcagggcc caagggctcg agaagagcta cgggaaacac aaggccgtca
      121 acggactgtc cttgcggtgc gcccccggcg aggtgatcgg ccttctcggc cccaacgggg
      181 cgggaaagac cacgaccttc tacatgatcg tgggcctcgt acgccccgac agcggagcgg
      241 tgtacctggg cgacgaggac atcaccgagt tcgacctcgc cggccgcgcg ctcaggggga
      301 tagggtacct gccgcaggaa ccgtcggtgt tcagggggct cagcgtggag gacaacatcg
      361 cggtcccgct ggaggcgaga ggcctgaaag gggcggacct caagggccgt gtcgacgaga
      421 tcgtgggggc gttcgggctg gaccacgtgc gcacgtccaa gggggtgtca ctctcggggg
      481 gcgagcgcag gagggtcgag atcgcccggg ccatggcgct cgacccgaaa tacatgctcc
      541 tcgacgaacc gttcgcgggg atagatccgg tcacggtcaa ggacatccag gagatgatca
      601 tcaccttgaa aaacagctcc atcggtgtca ttattacaga ccacaacgtg aaagaaacgc
      661 tcaagatctg cgacagggcc tacatcatcc accagggggt cgtgatagag gaagggaccc
      721 cggcgcaggt cgtggcgaac gaggacgtga gaaggtacta cgtgggaagg gatttctcgc
      781 tgtaagaggc agggagggag aagtggcact ggagctcaaa caacacctca agctggcgca
      841 acagctcatc atgacccccc agctccagca ggccatcaag ctcctgcagc tctccaggct
      901 cgagctccag gaatacatcg agaccgagct ccagcagaac ccgctgctgg aggtcgacac
      961 caccagggtc aaggaggaca ccctcgagga gatagcacag cgcaaggaac tcaggcagcg
     1021 atgggaggcc tacctggaga cctacggcca ggacaacata ccctactacg aagacgagga
     1081 aaggccgtct ttcgaggcca ccacctcgaa gccggaaggg ctcgtcgacc acctcatgtg
     1141 gcagctcagg ctcaacgact tcacggacga ggaacgcgcg atcgggctgt tcatcatagg
     1201 gaacctcgac cacaacgggt accttcccat ggacataccg tccatctgcc aggagaccgg
     1261 ggcttccgaa gaggtggtcg agcgtgtcct gtcgagggtc cagcagttcg acccgcaagg
     1321 catagccgcg agggacctca aggactgcct caagatccag gcacgccagc tcgggctgga
     1381 gaactccctc gtctggaaga ttatcgagaa tcacctccag gacctccaga ccaagaacta
     1441 ccacaagatc gcgcaggagc tgtccacgag cgtcgaagag gtggcacagg ccgccggcat
     1501 catcaccaac atggaaccca ggccggccag ggactactcg gacgacccgc cgcagtacat
     1561 cgtgcctgac gtctacgtgg tgaagctcga gggtgagtac gtggtgctcc tcaacgagga
     1621 ggacctcccc gtgctcaagt tcagccgcga gtaccaggag atgatgaagt cgaacagcat
     1681 gagcaagctc gcgagagact acctctccga gtgctacaag tccgcacagt ggctcatgaa
     1741 gagcattcag cagaggcaga acacgctcgt tcgagtcaca gagagcatcg tcaagttcca
     1801 gagagagttc ctcgaccacg ggatctcgca cctcaagccc ctcgttctca gggacgtcgc
     1861 cgacgacgtg gggatgcacg agtccaccat cagcagggtg acctccaaca agtacgtgca
     1921 tacgtgccag ggtaccttcg agctcaagtt cttcttcaac agcgggatct cgaagagcga
     1981 cgggagcttc gtcgcatccc agagcgtcaa gaacgagatc gaggggatca tcaaggcgga
     2041 ggaccccagg cacccgctga gcgaccaggc catcgctctc atcttaaagc agaagggggt
     2101 gagtatcgca cgcagaaccg tcgccaagta cagggaactc ctgggcattc ttccttccaa
     2161 ccagaggaag aaacatttct gaaagcccga attccaagag agggggatgt acatgctcac
     2221 aaacatcacc tttaagaaca tcgagtcgtc cgacgcactg agggattacg cactgaaacg
     2281 cctttcgaag atagacaagt acatagaccg cacggcagag gcgcacgtgg tcttttccgt
     2341 cgagaagcgc cgccacaagg ccgacatcac cctcagcgcg gacggaacgg tgatcaacgc
     2401 ggtggagatc accgacgact tgtatgcagc catcgacatg gtgatggaca agctcgaaag
     2461 acagctcaag aagcacaagg gaaagctcca ggacaagaag gcccccgcga agaccggcgt
     2521 gccggcgtct ttgtctgcgg aggaggcgcc gaaacccagg aagaagcccc cccgcatcgc
     2581 caagggaacc gcgtacacgg tcaggcccat gagcctcgac gaggccatcg aagagatgct
     2641 cgcaggcaag gaggacttcg tcctcttcag gaacacgaag tcgcagctgc tgaacctcct
     2701 ctacagacgc gcggacgggg agctggccat cgtcgagcct cggctatgaa aatcgctgac
     2761 atcctctccc ccgagtgcgt gatctcggat ctacggggcg ccacgaagaa ggagatcctc
     2821 gaggaactcg cgctgccggt atcccggatc caccgcatca acctcgacga gatcgtctcg
     2881 atcctcatgg cgcgggaaca gctggggtcg accgggatgg gagacggggt ggccataccg
     2941 cacggcaagg ttcccgggct gaaagacatc gtgggcgccc tgggcaagag cgccaagggc
     3001 ctcgatttcg attccataga caagaagccc tgccacatct tcttcatgct cctcgcgccc
     3061 agcacgttcg tgacccagca cctcaaggcc ctggccaggg tcgcaaccct cctgaaagac
     3121 ccctcgctga gacaccgcat catggagagc tcctctgcaa gcgagatcca tcacctgctc
     3181 atcgaccacg acagtaccat cgaggactga aggaaccaca cgaggcgcaa aaggggcggg
     3241 gcccgccgaa agcggcgggc cccgcccggt gtttcacggg tgtatgggtg tgcgacgcga
     3301 cccggatcag tcctcgtcgt cgtagccctt cggcttgagc gcctcggggg gcacttcgga
     3361 ggcgatggac ctgttgatgc ccctcgagag gatgaccgct atggtgaaga ggatgacagc
     3421 ggcgccgaag atgatgagca cgatcgtggt gagccaggcc tgggcctctt tctcgacctt
     3481 gcccgatgcc tccttggcct gctcgaggaa cttctcgacg tcgaccgcca tggccaccca
     3541 cccgaagccc gcgggcttgg ggtaatccct cgtatagaag ggcacgggcg cgtaggccac
     3601 gaccttggtg tgtccctcga actgataggt cttcaccccg gacaaaccct gagcggcatc
     3661 cttggccacc tcggggagtg tctggtcgac ccctcccagc tcgttcacgt tcaggacttc
     3721 ctcgcccttc ttcatcttct catcccaggt gcccttggtg atggggggca cctgcgtgcc
     3781 gtccttgtag aggccgacga tgtggtaatc agcagggtgc gaaatcacga acccgcggtt
     3841 gtccaccagg taagcatagt tgccggtggc cgcgtcgctc tccaccacgt ggcccgcctc
     3901 tgtggggacg acggtgtcgg tgaacctcga caacgccctc gcgtcgagcg taagcgtcag
     3961 gacccccgtg aatccccgct tgtcgaacac cggggttgcc agcctgatga tcccctcgaa
     4021 tctcttccct ttctcgaact cctccctcgt cacgtaccag cccgtgacgt gagagacgta
     4081 cacttcgccc ttgtcgagct ccctggcctt gatgaagtag tcctcgctct tgtacgtggt
     4141 gttcgccggg ttcgagatgt ccagcaaccc gtcagcgcct acgaccttgc cgtccttgat
     4201 cttcatgatc tcgcggcccg cgctgtcggt aagggagagc tcgacgtaca gggggagctg
     4261 ctctctcgcg atcccggtgt ctttcttgac ccagagctcg aacgtcttgg tgtccaggaa
     4321 cttcttgtag gcctgggcgt ccgccggaag gatggtggcg atgagaacgt ccttctggcg
     4381 ttcgcggagg aagtccgcca cgttctgggc cacgtcggcg gccctgacac ggagttcctg
     4441 ctgtgccttc tgatccagca ccgccatggt gcccttcttc gacacacctc cgatatggaa
     4501 gatgctgaag gcgataaggg aagctatcag taataagggg atgatcacga gcaggaaaaa
     4561 caaccgcgca accgttagaa ggcgacttcc tttgatcgtt tcagtcattg ttttccccct
     4621 tattatgcat agcgttatgg ttggacatac ctccgcaggg cgttatccga agaggaacgt
     4681 cttgtagggg ttcgcgaaga tcagaatgag cgtgatgacg agcgcataga tggcgatcga
     4741 ctcggccatg gccatgccca ccatcatggt catcatgatc ttgccctgcg cagcggggtt
     4801 ccttccaacc gcctccgacg cacctgcggc cgcgttccct atgccggcac cggcgccgat
     4861 gcaaccgagc ccgatgg
//
LOCUS       DAOSCR010000131         4867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_10950_length_4867_cov_4.131338, whole genome shotgun sequence.
ACCESSION   DAOSCR010000131 DAOSCR010000000
VERSION     DAOSCR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_4867_cov_4.131338"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLT69_04570"
     CDS             complement(<1..304)
                     /locus_tag="PLT69_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13749.1"
                     /translation="MDMRVYIVGETLGGRLTEETMDLASFVHSAYGRMPDACFLPSCK
                     GDDAAHELAGLTGSTVHLLRSGDDLCPPGRALAEKIAAIIGEGRPSAVFLAQRFFGL"
     gene            complement(314..1096)
                     /locus_tag="PLT69_04575"
     CDS             complement(314..1096)
                     /locus_tag="PLT69_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ13750.1"
                     /translation="MKVLVCIKQVVDAHDTVSLDEAAGRLTFAPKSVFRMNRADEHAL
                     EEALLLKERLGEDVEIHAVTVGPPRAASVVRRALEMGATGCVHVTVGDDGQPDPSTTA
                     RALAACVSRGGFDLVLAGVMAEDDMAGVTGQLVGAYLGWPCATSVVAIETLSPGGKVR
                     VTSEIEHGYRALREIALPAVLTIQTGINQPRYPRLSDVLRARTMSPETIPLDELVPHQ
                     RIGNAPKVAKPASSRQGIVLKGTSLEKARALVDILREKALIP"
     gene            1214..1924
                     /locus_tag="PLT69_04580"
     CDS             1214..1924
                     /locus_tag="PLT69_04580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ13751.1"
                     /translation="MSMIYLFRHGQASFGDDDYDVLSPRGLAQARILAGILYDKGFMP
                     VAVYSGTMRRQVSTAQEVLRFFKDSDRPLPGLVTMPEFDEYDTAAIVTALFPAMAAED
                     PSLEADLPRMYESKAAFKRIFEAAMLRWVSLGAHVDGVESWEGLKSRVMRGVDSIRRR
                     HGRGSTVAVFTSGGVIAACLAAVLGIPGETAMRLNWQIVNTSVTRLMYNEERVTLAGF
                     NDISHLELARDESLLTYR"
     gene            1954..3162
                     /locus_tag="PLT69_04585"
     CDS             1954..3162
                     /locus_tag="PLT69_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13752.1"
                     /translation="MDFSVSERVQAITSLIDEFVDKELIPLEREFLHKSCREMLPVLE
                     KKRDMVRKMELWAPNHPKEYGGMGLGLVDHAFVSECLGRTPIGHYVFNCQAPDAGNIE
                     ILHLHGTPEQKERYLRPLVEGKIRSCFSMTEVEMPGSNPVMMDTTAVKDGDDYVINGQ
                     KWYSTAADGAAFSIVMAVTNPEAPPYLRASMIIVPTDTKGFNLVRNIPVMGHTGDDYA
                     SHGEVLFQSCRVPQKNLLGPEGHGFVIAQERLGPGRIHHCMRWIGICNRAFDLMCSRA
                     SSRIIQPDGRTLATKQIIQAWIAECAANIQAARLMTLNAAWKIETMGAKAARYDISFI
                     KFFVANVMQEVIDKALQVHGGLGMTDDTIIAYFYRHERAARIYDGADEVHKMAVAKRI
                     LRDYEGRTVR"
     gene            3165..3881
                     /locus_tag="PLT69_04590"
     CDS             3165..3881
                     /locus_tag="PLT69_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13753.1"
                     /translation="MAKGELSFEEFRRMVSLSMENMCRDLYLGNRASIKIKKEPVAVG
                     NLMKILDAALTLSNKKGFSAMSLRELSSQSGLSMGALYTYFSSKEDLLHMIQRESAVV
                     FDVLTAQLEGVRDPQARLRRAIRTHLFLSEILRPWFYFSYMEAKNLSKQEQKKAIEAE
                     LLTEKLFVDIVREGQDVGVFRDCDAELVAAVTKAMLQDWYLKRWKYARRKTTVERYAE
                     FVTDVVEGYLGCGRRGEKKS"
     gene            3894..>4867
                     /locus_tag="PLT69_04595"
     CDS             3894..>4867
                     /locus_tag="PLT69_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOJ13754.1"
                     /translation="MIDTPTKVRQGEELDLKAVEAFLKDSIPGLHGELVVEQFPSGYS
                     NLTYLIRVGERELVLRRPPFGRKAKTAHDMGREYRILKALNPVFPYCPRPLVYTEDPS
                     VMGCPFYVMDRIRGIILRKNLPEGLELSPGQVRTLCENLLDVQLELHAVDYKAIGLEG
                     FGRPEGYVRRQVEGWSERFRAAWTPDAPTFDGVMQWLHDRMPPDSQRPALIHNDYKFD
                     NVVLDPKDPLRIIGVLDWEMATIGDPLMDLGSSLGYWVQADDPEDFQAARMMPTNLPG
                     ALTRKEMVERYAEKAGITIDNFDFYLCFGLFRLAVIAQQIYYRYYHGQT"
BASE COUNT          952 a         1504 c         1591 g          820 t
ORIGIN      
        1 cgagcccgaa gaacctttgc gccaggaaca cggcagacgg ccgcccttcg ccgatgatgg
       61 ccgcgatctt ctccgccagg gcgcgcccag gcgggcacag gtcgtctccg gacctcaaga
      121 ggtggacggt ggacccggtg aggccggcaa gctcatgcgc cgcgtcatcg cccttgcaag
      181 agggcaggaa gcacgcatcg ggcatgcggc cgtacgcgga atgcacgaag gaagcgaggt
      241 ccatcgtttc ctcggtgagc cttccgccga gggtctctcc cacgatgtac acgcgcatgt
      301 ccatgagggt ctcctagggg atgagggcct tctcgcgaag gatgtcgacg agagccctgg
      361 ccttctcgag ggatgtcccc ttgaggacga tgccctgcct gctgctcgcg ggcttggcga
      421 ccttcggtgc attcccgatc cgttggtgcg gcaccagttc gtccaagggg atcgtctccg
      481 gagacatggt cctggcgcgc aggacgtccg agagcctggg gtagcggggt tggttgatcc
      541 cggtctggat ggtgagcacg gcgggcaggg cgatctcgcg cagagcgcgg tacccgtgct
      601 cgatctcgct cgtgacccgc accttccctc ccggggaaag cgtctcgatc gcgacgaccg
      661 aggtcgcgca gggccatccg aggtacgccc ccacgagctg gccggtgaca cccgccatgt
      721 cgtcctctgc catgacgcct gcgagaacga ggtcgaagcc ccccctggat acgcatgcag
      781 caagggcccg ggccgttgtc gagggatcgg gctgcccatc gtcgccgacc gtcacgtgca
      841 cgcacccggt cgcccccatc tcgagggccc gcctgacgac agaggcggcc cttggcgggc
      901 cgacggtcac tgcgtggatc tcgacgtcct cgccgaggcg ctccttgagg aggagcgcct
      961 cttccagggc atgttcgtcc gccctgttca tcctgaagac gctctttggg gcgaaggtga
     1021 gcctgcccgc tgcctcgtcg aggctcacgg tgtcgtgggc gtcgaccacc tgctttatgc
     1081 acacgagaac cttcatgctc tatctccgct cgcactctac catacctcaa ggcgcaggat
     1141 gcaggcaagc aaaaaacgaa cgttcgttat atttagcctt gacaggcatc cctttccagg
     1201 gtaggatcgg tccatgagca tgatatacct cttcaggcat gggcaggcct ccttcgggga
     1261 cgacgattac gacgtccttt ctccgagggg gcttgcgcag gccaggatcc tcgcggggat
     1321 cctctacgac aagggcttca tgccggttgc cgtgtactcc gggaccatga ggcggcaggt
     1381 gtccaccgcc caggaggtcc tgaggttctt caaggacagc gacaggccgc tgccgggcct
     1441 ggtgacgatg ccggagttcg acgagtacga caccgcagcc atcgtgaccg ccctgttccc
     1501 ggccatggcg gcggaggacc cctctctcga ggcggatctg ccgaggatgt acgagagcaa
     1561 ggccgcgttc aagcggatat tcgaggcggc catgctcagg tgggtgtccc tgggcgccca
     1621 tgtcgacggc gtggaatcgt gggaggggct caagagccgt gtcatgcggg gggtggactc
     1681 cataagacgg cgtcacggca gggggagcac cgtggcggtc ttcacctcgg gcggggtgat
     1741 cgccgcatgc ctggccgcgg tgctgggaat cccgggggag accgccatgc gactgaactg
     1801 gcagatcgtc aacacctcgg tcacgaggct catgtacaac gaggagaggg taaccctggc
     1861 cgggttcaac gacatctccc acctcgagct cgcccgggac gaatcgctcc tcacctatag
     1921 gtgaggggcc ttcacaaacg aaaggagaca gccatggatt tctcggtttc cgaaagggtg
     1981 caggccatca catccctgat cgacgagttc gtcgacaagg agctgatccc cctcgagagg
     2041 gagtttctcc acaagagttg ccgcgagatg ctgccggtgc tcgagaagaa gcgggacatg
     2101 gttcgcaaga tggagctctg ggcgccgaac caccccaagg aatacggcgg catggggctc
     2161 ggcctcgtgg atcacgcctt cgtctccgag tgcctgggaa ggacccccat cggtcactac
     2221 gtcttcaact gccaggcccc ggatgcgggg aacatagaga tcctccacct ccacggcacc
     2281 cccgagcaga aggaacgcta cctgaggccc ctcgtggagg ggaagatcag gagctgcttc
     2341 tccatgaccg aggtggagat gcccgggtcc aacccggtca tgatggatac caccgcggtc
     2401 aaggacggcg acgattacgt gatcaacggg cagaagtggt actccacggc agccgacggt
     2461 gctgccttct ccatcgtcat ggccgtcacg aacccggagg ccccgccgta tttgagggcg
     2521 agcatgatca tcgtgcccac cgacaccaag gggttcaacc tcgtgaggaa catcccggtc
     2581 atggggcaca cgggcgacga ttacgcatct cacggcgagg tgctcttcca gtcctgccgg
     2641 gtgccgcaga agaacctgct gggtcccgag gggcacgggt tcgtcatcgc gcaggaacgg
     2701 ctgggcccgg gcaggatcca ccactgcatg cgatggatcg ggatctgcaa ccgcgccttc
     2761 gacctcatgt gctcgagggc ctccagccgc atcatccagc ccgacggcag gaccctggcg
     2821 accaagcaga tcatccaggc gtggatagcc gagtgcgcgg cgaacatcca ggccgcccgc
     2881 ctcatgacgc tgaatgcggc gtggaagatc gagaccatgg gcgcaaaggc ggccaggtac
     2941 gacatctcgt tcatcaagtt cttcgtggcg aacgtgatgc aggaagtcat agacaaggcg
     3001 ctccaggtcc acggaggcct cggcatgacc gacgacacca tcatcgccta cttctaccgc
     3061 catgagcggg cggcgcgcat ctacgacggc gcggacgagg tgcacaagat ggcggtggcc
     3121 aagaggatcc tgagggacta cgaaggcagg acggttcgtt agtcatggcc aagggcgaac
     3181 tctcgttcga ggaattcagg cgcatggtct ccctctccat ggagaacatg tgcagggatc
     3241 tctacctcgg caaccgcgcg agcatcaaga taaagaagga accggtcgcg gtgggaaacc
     3301 tcatgaagat cctggacgcc gcgctcaccc tcagcaacaa gaagggcttc tccgccatga
     3361 gcctgaggga gttgagcagc cagagcggtc tcagcatggg ggctctctac acgtacttct
     3421 cgagcaagga agacctcctt cacatgatcc agcgcgagag cgcggtcgtg ttcgacgtcc
     3481 tcaccgctca gctcgagggg gtacgagacc cgcaagccag gctcaggagg gccatacgca
     3541 cccacctctt cctgagcgag atcttgaggc cgtggttcta cttctcctac atggaggcca
     3601 agaacctctc gaagcaggaa cagaagaagg ccatcgaggc ggagctcctc accgagaagc
     3661 tcttcgtgga catcgtgcgc gaaggccagg acgtaggcgt cttcagagac tgcgacgcag
     3721 agctcgtggc cgcggtcacg aaggccatgc tccaggactg gtatctcaag aggtggaagt
     3781 acgcgaggag gaagacgacg gtggagaggt acgccgagtt cgtgacggat gtcgtcgagg
     3841 ggtacctcgg gtgcgggcga cggggagaaa aaaagagctg aaggggtgat cacatgatcg
     3901 acacgccgac caaggtgcga cagggagagg agctcgatct caaggccgtc gaggccttcc
     3961 tcaaggactc gatcccgggt cttcatggcg aactcgtggt ggagcagttc ccgagcgggt
     4021 actcgaacct gacgtacctc atacgggtcg gggagaggga gctcgtgctc aggaggccgc
     4081 cgttcggccg caaggcgaag acggcccacg acatgggcag ggaataccgc atcctcaagg
     4141 cgctgaaccc ggtgttcccc tactgcccga gaccgctcgt ctacaccgag gatccgtcgg
     4201 ttatggggtg ccccttctac gtgatggaca ggatcagggg gatcatcctg aggaagaatc
     4261 tccccgaggg tctcgagctc tcccccggcc aggtgcgcac gctgtgcgag aacctcctgg
     4321 acgtgcagct cgagcttcac gccgtggact acaaggccat aggcctggaa ggcttcggca
     4381 ggcccgaggg atacgtgagg cgccaggtgg agggctggtc ggagcggttc agggcggcgt
     4441 ggaccccgga cgcaccgacc ttcgacggcg tcatgcagtg gctccatgac aggatgccgc
     4501 ccgactctca gaggcctgcg ctcatccaca acgactacaa gttcgacaac gtcgtccttg
     4561 acccgaaaga ccccctgagg atcatcggtg tcttggactg ggagatggcc accatcggcg
     4621 atccgctcat ggacctcggg agttcgctcg gctactgggt gcaggcggac gaccccgagg
     4681 atttccaggc cgcccgcatg atgcccacga acctgccggg tgcgctcacg cgcaaggaga
     4741 tggtggagag gtatgccgag aaggcgggga tcaccatcga caacttcgac ttctacctct
     4801 gtttcgggct cttccgcctg gcggtcatag cccagcagat ctactaccgc tactaccacg
     4861 gacagac
//
LOCUS       DAOSCR010000132         4835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11043_length_4835_cov_3.461925, whole genome shotgun sequence.
ACCESSION   DAOSCR010000132 DAOSCR010000000
VERSION     DAOSCR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11043_length_4835_cov_3.461925"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1493)
                     /locus_tag="PLT69_04600"
                     /pseudo
     CDS             complement(30..1493)
                     /locus_tag="PLT69_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
     gene            complement(1522..2862)
                     /locus_tag="PLT69_04605"
     CDS             complement(1522..2862)
                     /locus_tag="PLT69_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOJ13755.1"
                     /translation="MDKPKKVKKPRGRASLIPNKCIACGARCQSSCPVEAIEMNEKGE
                     PIIDQAKCIGCQKCIKVCPAQALEMYFTPEELEILKQLAAEKGAAGAEEEEDSEEAKL
                     RKYLDQYRGVWVFVEQTDCIPAKVSWELLGVGAQLASALQVELCAVVLGHNVEKLCHE
                     AFAYGATKAYLIDDPILHHYRTAPYVEGFCSLVEKYKPEVVLMGATGLGRDLAGAVAT
                     RLQTGLTADCTGLGIDEKRQLLQTRPAFGGNIMATIFCEQRRPQMASVRPHVMPMPAR
                     KEGAEGTIVRETISMKEEDILTKVLEVITDASHKDSIDISGAEFIVSGGRGMMAKENF
                     RILQELADELGGVVACSRSAVDAGWMPVERQVGQTGKTVRPKIYIACGISGAIQHLVG
                     MQDSDLIIAINRDKEAPIFEVATYGIVGDLFQVVPAITEYIRKAKATRGTPKVC"
     gene            complement(2916..3728)
                     /locus_tag="PLT69_04610"
     CDS             complement(2916..3728)
                     /locus_tag="PLT69_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ13756.1"
                     /translation="MLVVACIKQVPDTTQVKIDPVTNTLVREGIPFIMNPFDTHALEE
                     ALRLKDRFGVRVAAVSMGPPNAEMTLRKALSLGADEVVLLSDRAFGGADTLATSMVLA
                     EAVKRLAEKDELALVICGKQTIDGDTAQVGPGIAMRLNLVPLTLVDRIVDLDLAAKTI
                     VVSRKLEARHEIVQSKLPALLTVVREINKPRYPSVPMRLEAQDLPVQLWDNKVMNLDV
                     NKIGLKGSPTAVRKIFSPEREQGEIIGDGMKDPEGTAKLLVEKLVQKELLAL"
     gene            4563..>4835
                     /locus_tag="PLT69_04615"
     CDS             4563..>4835
                     /locus_tag="PLT69_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ13757.1"
                     /translation="MRTRGCSHRRLLLVRGGSIASGVGVGKGYTDHLEALCRAKGVEF
                     VNVSRPGETSFDGVWTFDEDIAPWRPDILVIHFGIDDAFHPVYRSEF"
BASE COUNT          839 a         1533 c         1500 g          963 t
ORIGIN      
        1 cgttactgga tgatgaaagc cccgaaaagg accttcttca cgcccttttc cttgaagagc
       61 ttcacgttct ccctcgccat cctgtcgaag acgaactcgt tgccgagcct cctgagggaa
      121 tcgccgcagc agagctcctc cttgccgagg atgccgtagg agacgccggc cgcgtccaag
      181 atgtgggcga gcgccacggt gacctgcttg ttgcgggagt cgaaggcgcc cgcgcacccc
      241 acgtagagga ggtactcgca ggaggcgtcg aaaggcttca cgttgagctg ggagcaccac
      301 ttggtgcgct cggtgggtgc tatgccccag gggttgctcc gttgctccat gttctcgaag
      361 aggttgagga gctcctcggg gaacgtggcc ttcatctcca cgagatagcg cctgagctgc
      421 aggatcttcg gcatctgctc gatgaagacc gggcacacct ccatgcatgc gccgcaggtc
      481 gtgcaggacc agatctcgtc ctcggttatc gacccctcgc cgttgttgcc tatgagcggg
      541 agggtcggct tcccgccttc cttaatgatc ggcccgttgg cgagcaggtt gaccttgagg
      601 tcgtggatca ccttccgggg attgagcggc ttgcccgtgt tcgaggccgg gcacaccatc
      661 tggcagcggc cgcactccgt gcaggagtac gagtcgaaca ggtccttcca cgtgtactgg
      721 tccacggtct ccacgccgaa cttggcgccc ttgacgaagg tctcggtcgg cagcgtgttc
      781 gccttgccga acctcctgaa gtaacagttc gggatggagg acaggatgtg catgtgcttg
      841 ctgtacggca ggtagttcag gaacacgagg aacacgatgg cgtgcagcca ccaccagaag
      901 tttatgaagg agtgcgccga ttccagatcg atccccgcgt agaaggtccg gccgacccac
      961 gcggagatgg gcatgagggc cgagatctcc cccacggcca gttccacgcc gtggaggttg
     1021 aagtaggcga tcatgagcag ggcgatcatg gagaggatca cgtacgcgtc cagcgtgatg
     1081 gcctcggggt acgggggggc caccaccctc ctgacgcccg ccacgatcac gcacaccagc
     1141 gcgaagaggc tgaagagatc gaagaggaag tatatcgggt tgtggatgaa ggccggcagg
     1201 agcatgaaag acagggagtg gtgaagaccc ttgagcacga actctatgtt ggcgagcagg
     1261 agcaccatga aggaccagaa caggacgaaa tggttgatgc cgaagggctt ctgcaagacc
     1321 ctcttctggc agaacgcgta gaggaaggtg tcccgtatcc tggcggggaa attgtcgaag
     1381 cggtcttcgg gcttgccgat ggtgacgagg ctgaagcggc gcacgcagct ccagatgaac
     1441 aacaagaccg ctgccacaaa cacgatggcg aaaatcttcg agttcggtgt catgaacgta
     1501 tcctctttcg tagtatcctg atcagcagac cttcggcgtc ccccgggtgg ccttcgcctt
     1561 cctgatgtac tcagttatcg ccgggacgac ctggaagagg tcgccgacga tcccgtaggt
     1621 ggccacctcg aaaatgggcg cctccttgtc gcggttgatg gcgatgatga gatctgagtc
     1681 ctgcatcccg acgaggtgct gtatggcgcc ggatatgccg caggcgatgt agatcttggg
     1741 cctcaccgtc ttcccggtct ggccgacctg tctctcaacg ggcatccagc ccgcgtccac
     1801 cgcgctcctg gagcacgcca ccacacctcc gagctcgtcg gcgagctcct ggaggatcct
     1861 gaagttctcc ttcgccatca tgcccctgcc gcccgacacg atgaactccg cgccggatat
     1921 gtcgatgctg tccttgtgcg atgcgtcggt gatcacctcg aggaccttgg tgaggatatc
     1981 ctcttccttc atgctgatgg tctcgcgcac gatggtcccc tcggccccct ctttcctggc
     2041 cggcatgggc atgacgtgcg ggcgcaccga ggccatctgc ggcctcctct gctcgcagaa
     2101 gatggtcgcc atgatgttcc cgccgaaagc gggtcgcgtt tggagaagct gtctcttctc
     2161 gtcgatgccg aggccggtgc agtcggcggt gagccctgtc tgcagcctcg tggccacggc
     2221 gccggcaagg tccctgccga ggccggtcgc acccatgagc accacttctg gcttgtactt
     2281 ctccaccagt gagcagaacc cttccacgta gggcgcggtc cggtagtggt gaaggatggg
     2341 gtcgtcgatg aggtatgcct tggtggcgcc gtaggcgaac gcctcgtggc acagcttttc
     2401 gacgttgtgg ccgaggacca ccgcgcagag ttccacctgc agcgccgacg cgagctgggc
     2461 gcccacgccc aggagctccc aggaaacctt ggccgggatg cagtcggtct gttccacgaa
     2521 gacccacacg ccccgatact ggtcgaggta cttcctgagc ttcgcctctt ccgagtcttc
     2581 ctcttcctcg gcccctgcgg cgcccttctc ggcggcgagc tgcttgagga tctcgagttc
     2641 ctcgggggtg aagtacatct cgagggcctg cgccggacag accttgatgc acttctggca
     2701 gccgatgcac tttgcctggt cgatgatcgg ctcgcccttc tcgttcatct cgatcgcctc
     2761 gacggggcag gagctctggc accgggcccc gcaggcgata cacttgttgg gaatgagaga
     2821 cgccctacct cgcggcttct tgaccttctt cggcttgtcc atatcctgtc gtcctgtctc
     2881 tttgtgtctt tatgttccgg ccttcacacc gagtgctaca gcgccaggag ctctttctgg
     2941 acgagcttct cgaccaggag cttcgctgtg ccctcgggat ccttcatccc gtcgcctatg
     3001 atctcgccct gctcgcgctc gggcgaaaag atcttgcgca cggcggtggg agaccccttg
     3061 agcccgatct tgttcacgtc gaggttcatg accttgttgt cccagagctg aaccggaagg
     3121 tcctgggcct cgaggcgcat ggggacgctg gggtacctgg gcttgttgat ctccctgacg
     3181 accgtgagga gcgcgggcag cttcgactgc acgatctcgt gacgcgcctc gagcttcctg
     3241 ctgaccacga tggtcttggc agcgaggtcg agatccacga tgcggtccac cagggtcaag
     3301 ggaacgagat tcaacctcat ggcgatgccg gggcccacct gggcggtgtc cccgtcgatc
     3361 gtctgcttcc cgcagatcac gagcgcgagc tcgtccttct cggcgagcct cttgaccgcc
     3421 tccgcaagga ccatgctcgt ggcgagcgtg tcggcgccgc cgaaggccct gtcggaaagg
     3481 aggaccacct cgtccgcccc cagggacagc gccttcctga gcgtcatctc tgcgttcggg
     3541 ggccccatgg acacggccgc caccctcacc ccgaacctgt ccttgagcct gagggcctcc
     3601 tcgagggcat gggtgtcgaa ggggttcatg atgaagggga tcccctcgcg gaccagggtg
     3661 ttggtgaccg gatcgatctt gacctgcgtg gtgtctggca cctgcttgat acatgcgact
     3721 acgagcattg gctaccttcc ttcatggttc atcccgggac gggatgttgt tgtacacgtt
     3781 ccgttctcca tggacaggat ccactccctc gcagtaccga cggccgtgtt gcaaaaacgt
     3841 gtcccaccga acgaatcagc gaccggccat gagtgaacca tcattcatca ccgggtgaac
     3901 atacatgatg gtctcgattt ctgtcaacac cattttcatg cagcgtgcac ccgtggacgt
     3961 tgaggcgagc gtttataggt aactatcgta gagatttccg ttcagggaca cgagaccccc
     4021 gcctggcgtc tcccgggtcc aggtccgtca gatctcccgc tcgggcgctc atggggcacc
     4081 gggcatgccc tctcccgaac cggaagagct caaacccttt tccacgcagg ccctcacccg
     4141 tacccgtaac cggcaaggcc gcatcatccc catcgccgct tcccgaggct acgcgcttca
     4201 tcgccatcca aggctcctcc cataacctct cccccctcgc ctcgaccctg acccgtccgg
     4261 gccacgaagt cggacgcctt cagcgcgggc aggttttccc ccgccagggg gtatcgtctc
     4321 ttgcatcgcc aaggtgtata tgagagaggc ggccgggccg tgcgggagac gtgcacgccg
     4381 cagggcggcc tcgtcggccg tcatgtcacg ggtctccctg cgtgccagcc gttgcttgtc
     4441 cctgccgtgg cggccgggga tacgaccccg cccttccgtg acaggcgggc ctgtccccgg
     4501 agtggagcgg acaggcgcgg cacccggccg acgcaccagg gtgtctcgag gaggtcacga
     4561 tcatgagaac aaggggatgt tcccacagga ggctcctcct cgtgaggggg ggaagcatag
     4621 cctccggcgt cggagtcggg aagggataca cggaccacct cgaagcgctg tgcagggcga
     4681 agggcgtgga gttcgtgaac gtctcgaggc cgggcgagac gtccttcgac ggcgtctgga
     4741 ccttcgacga ggacatagcg ccgtggagac cagacatcct cgtcatccac ttcggcatcg
     4801 acgacgcctt ccaccccgtc taccgatcgg agttc
//
LOCUS       DAOSCR010000133         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11135_length_4811_cov_3.219302, whole genome shotgun sequence.
ACCESSION   DAOSCR010000133 DAOSCR010000000
VERSION     DAOSCR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11135_length_4811_cov_3.219302"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLT69_04620"
     CDS             <1..442
                     /locus_tag="PLT69_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HOJ13758.1"
                     /translation="DEAGMPVEATTLSIVRREGVCASMPSRYERAAPLRGTKNTIVRT
                     RKVYAGIGRSDNASIVVIPLEDARRVITHLLLLQVDFDEHLQTARKKDVLGVKLNDIV
                     NLVNEYDIPWKDEYLEHFDIKVLLGEDVNDIVGEIVKVHKERGR"
     gene            504..3728
                     /gene="carB"
                     /locus_tag="PLT69_04625"
     CDS             504..3728
                     /gene="carB"
                     /locus_tag="PLT69_04625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ13759.1"
                     /translation="MPKRDDLKKILIVGSGPIVISQACEFDYSGVQACKALREEGYEV
                     VLVNSNPATIMTDPGMADRTYIEPITADYLELIVARERPDAILPTLGGQTGLNVSVEL
                     ASRGVLERYGVEMIGARPEVIHRAEDRELFRKAVEEAGLRVPRSGFARSMDEAFRIAE
                     SIGYPVIIRPSFTLGGIGGGIAYNPEDLGRIAKEGLDASMIREIMIEESVIGWKEFEL
                     EVMRDRADNVVIICSIENVDPMGVHTGDSITVAPAQTLTDVEYQQMRDAAIRVMRAIG
                     VETGGSNVQFAVNPSNGELVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETFASFEPAIDYCVVKIPRWTFEKFPETADVLTTSMKSVGEVMAVGRTFKE
                     ALNKAVRSLEIGSYGLGWPRTIETPEDKKAVVKGLSYPNSKRLFSMACALRSGFTVED
                     VSRLTGIDPWFVGQIEDLVKAERVIRDAGLEEGVLLEAKRMGFSDRYLAALWDTTEEH
                     VRSERHRLGVTPVYKLVDTCAAEFEAYTPYYYSTYEQEDESNPSGARKVIILGSGPNR
                     IGQGIEFDYCCVQASFALRESGYESIMINSNPETVSTDYDISSKLYFEPLTLEDVLHV
                     VSRERPEGVIVQFGGQTPLNLTKGLADAGVKILGTQPDAIARAEDRRLFLEIVNKLRL
                     KQPPSDTATTTREAVAIADRIGYPVLVRPSYVLGGRSMEIVYDKDALESFMAGVLELF
                     PDNQILIDKFLEDALEVDVDSICDGSRCVICGVMEHIEEAGIHSGDSSCVLPPPTLDK
                     RVVARIEDQTKRIALELGVRGLMNVQFAVKGSDVYVLEVNPRASRTIPFVSKAVGVSF
                     ARLATKVMLGCTLDELGLRRKRTPRYVSVKESVFPFARFPGITVALGPEMKSTGEVMG
                     IDSSFELAFAKAQCSAGFELPTSGNVFVSVHDAYKGRVVDIVRSFSDMGFGIIATTGT
                     ASYLSGKGVQVDTVLKVSEGRPNVADAIMNGQIQLVINVSLGRRPTMDAALLRRHAIR
                     AQVPYTTTIAAAKAIARAIGVLKKTTPGVKTLQEYHARTSKPRT"
     gene            3847..>4811
                     /locus_tag="PLT69_04630"
     CDS             3847..>4811
                     /locus_tag="PLT69_04630"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOJ13760.1"
                     /translation="MPTNKSTKYIFITGGVLSSLGKGLAAASIGAIMEARGLKVCNIK
                     LDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTSSTLSAKNNFTTGQIYDA
                     VIQKERNGEYLGKTVQVIPHITDEIKARIEAAAVGHDIAIVEIGGTVGDIESLPFLEA
                     IRQFGFDKGRGNVLYIHLTLVPYIATAGELKTKPTQHSVQKLREIGIQPAIILCRSER
                     RLTSELKEKIALFCNVEKSAVINAVDVQSVYEVPIEFHREGLDDRIVEYLNMWTREPR
                     MQGWEDLVERIRKRTEKVNIAVVGKYVRLTDSYKSLNEALEHAGIA"
BASE COUNT          991 a         1456 c         1607 g          757 t
ORIGIN      
        1 ggacgaggcg ggcatgcccg tggaggctac cacgctctcc atcgtcaggc gcgagggcgt
       61 gtgcgcgtcc atgccgtcca ggtacgagcg cgcggccccg ctcaggggga ccaagaacac
      121 catagtcagg acccggaagg tgtacgccgg tatcgggcgg tcggacaacg cttccatcgt
      181 ggtcatcccc ctggaggacg cgcgccgcgt catcacccac ctcctccttc tgcaggtgga
      241 cttcgacgag cacctccaga cggccaggaa gaaggatgtt ctcggcgtca agctcaacga
      301 catcgtgaac ctcgtcaacg agtacgacat accctggaag gacgagtacc tggaacactt
      361 cgatatcaag gtgttgctcg gcgaggacgt gaacgacatc gtgggggaga tcgtcaaggt
      421 gcacaaagaa aggggtcggt aaaccttcgg tgacctgaag cggtcacgga ccccagacag
      481 ccggagcgaa acagaagaca gccatgccca agagagacga cctgaagaag atactcatcg
      541 tggggagcgg gcccatagtc atatcgcagg catgcgagtt cgactattcg ggggtgcagg
      601 cctgcaaggc gctgcgggag gaagggtacg aggtggtcct cgtgaactcg aaccccgcca
      661 ccatcatgac cgaccccggg atggccgacc gcacctacat cgaacccatc acggccgact
      721 acctcgagct gatcgtagcc agggagcggc cggacgcgat cctgccgacc ctcggggggc
      781 agaccggcct caacgtctcc gtggagctcg catccagggg cgtcctcgag cgctacgggg
      841 tggagatgat cggtgcgagg cccgaggtca tacaccgcgc agaggacagg gagctcttca
      901 ggaaggccgt ggaagaagcg ggcctcaggg tccccaggag cggcttcgcc aggagcatgg
      961 acgaggcgtt caggatagcc gagtcgatcg gctaccccgt catcatcagg ccgtccttca
     1021 cgctcggcgg catcggcggg gggatcgcgt acaaccccga ggacctgggc cgcatagcca
     1081 aggagggcct cgacgcgagc atgatccgcg agatcatgat cgaggagtcg gtgatcgggt
     1141 ggaaggaatt cgagctcgag gtgatgcggg acagggcgga taatgtggtg atcatctgtt
     1201 ccatcgagaa cgtcgatccc atgggggtcc acacggggga ctccatcacg gtggccccgg
     1261 cgcagacgct caccgacgtg gaataccagc aaatgaggga cgcggccata cgcgtcatgc
     1321 gagccatcgg ggtcgagacc ggcgggtcga acgtccagtt cgccgtgaac ccctcaaacg
     1381 gcgagctcgt ggtgatcgag atgaatccca gggtctccag gtcctctgcg ctcgcatcca
     1441 aggcgacggg gttccccata gccaagatcg cggcgaagct ggcggtcggg tataccctgg
     1501 acgagatccc caacgacatc accagggaga ccttcgcgtc gttcgagccc gccatcgact
     1561 actgcgtggt gaagatcccc aggtggacct tcgagaagtt ccccgagacc gcggacgtcc
     1621 tcaccacgtc catgaaatcc gtcggggagg tcatggccgt gggcaggacg ttcaaggagg
     1681 cgctcaacaa ggcggtgagg tcgcttgaga tagggtccta cggactgggc tggccgagga
     1741 cgatagagac acccgaagac aagaaggcgg tggtcaaggg tctgagctac cccaactcga
     1801 agcgcctgtt ctccatggca tgcgccctca ggtcggggtt cacggtcgag gacgtctcca
     1861 ggctcacggg catcgacccc tggttcgtgg gccagatcga ggacctcgtg aaggcggagc
     1921 gggtcatccg ggatgcgggc ctggaggagg gggttctcct ggaggcgaag aggatggggt
     1981 tctcggacag gtacctggcg gcgctgtggg acaccacgga ggaacacgtg cgctccgagc
     2041 ggcaccggct cggcgtcacc cctgtgtaca agctggtgga cacctgcgcc gccgagtttg
     2101 aggcgtacac cccgtactac tactccacct acgagcagga ggacgagtcg aacccgtctg
     2161 gcgccaggaa ggtgatcatc ctcgggagcg gcccgaaccg gatagggcag ggcatcgagt
     2221 tcgactactg ctgtgttcag gcgtcgttcg ccctcaggga atcggggtac gagagcatca
     2281 tgatcaactc gaacccggag accgtgagca ccgactacga catctcgagc aagctctact
     2341 tcgagccgct caccctggag gacgtgctcc acgtcgtctc gagggaacga cccgaggggg
     2401 tgatcgtcca gttcggcggg cagacgccgc tcaacctcac caagggcttg gcggacgccg
     2461 gcgtgaagat cctcggcacc cagcccgacg ccatagccag ggccgaagac cgcaggctgt
     2521 tcctcgagat cgtgaacaag ctcaggctga agcagccgcc gagcgacacg gccacgacca
     2581 cgcgcgaggc cgtggccatc gcggaccgca tcgggtaccc cgtgctggtc aggccctcgt
     2641 acgtgctcgg ggggcgttcc atggagatcg tctacgacaa ggacgcgctg gagtctttca
     2701 tggccggggt gctcgaactc ttccccgaca accagatcct catagacaag ttcctggagg
     2761 acgccctcga ggtggacgtg gactcgatct gcgacgggtc caggtgcgtg atctgcgggg
     2821 tcatggaaca catagaggag gccggtatcc actccggcga ctcgtcatgc gtgcttccgc
     2881 cgcccacgct cgacaaacgc gtcgttgcac gcatagagga ccagacgaag aggatagcac
     2941 tcgagctcgg cgtgaggggc ctcatgaacg tgcagttcgc ggtcaagggg tccgatgtct
     3001 atgtgctcga ggtcaacccc agggcctcga ggaccatccc gttcgtgagc aaggcggtcg
     3061 gggtgagctt cgcgaggctc gcgaccaagg tcatgctcgg ctgtaccctg gacgagctcg
     3121 gcctgaggag gaagcgcacg cccaggtacg tctcggtgaa ggagtccgtg ttcccgttcg
     3181 cgaggttccc gggcatcacc gtggcgctgg gccccgagat gaagtccacg ggcgaggtca
     3241 tggggatcga ctcgagtttc gagctcgcgt tcgccaaggc gcagtgttcg gccgggttcg
     3301 agctgcccac ctccggcaac gtgttcgtga gcgtccacga tgcatacaag ggccgtgtgg
     3361 tggacatcgt gaggtctttc tcggacatgg gtttcggcat catcgcgacg accggtaccg
     3421 cctcgtacct ttccggcaag ggcgttcagg tcgacacggt gctcaaggtg agcgagggga
     3481 ggccgaacgt ggcggatgcc atcatgaacg ggcagatcca actcgtgatc aacgtcagcc
     3541 tcggcaggag gcccacgatg gacgcggcgc tcctcaggcg gcacgcgatc cgcgctcagg
     3601 tcccctacac caccacgatc gccgccgcca aggccatcgc cagagccata ggcgtcctga
     3661 agaagaccac gccgggggtg aagaccctcc aggagtacca cgccaggacc tcgaaaccga
     3721 gaacttgaaa ggcgtatccc tacctgatat gtgaggtgtc gggaaggttg agattgtccc
     3781 ggtgtggggt gcatccgggt ccccgacggc gcgcgcgggc cggcgacaag gaggagagga
     3841 ttgggtatgc ccacgaacaa gtccaccaag tacatcttca ttacgggcgg ggtgttgtcg
     3901 tctctcggca aggggcttgc ggcggcctcc atcggcgcca tcatggaggc gaggggtctc
     3961 aaggtctgca acatcaagct cgacccctac atcaacgtcg acccggggac catgagcccc
     4021 tttcagcacg gcgaggtgtt cgtgacggac gacggcgccg agacggatct cgacctcggc
     4081 cattacgagc gcttcacgtc gagcaccttg agcgcgaaga acaacttcac caccggccag
     4141 atctacgatg ccgtgatcca gaaggagcgg aacggcgagt acctcggcaa gaccgtccag
     4201 gtgatccccc acataaccga cgagatcaag gcgcgcatcg aggccgccgc cgtgggtcac
     4261 gacatcgcca tcgtggagat aggcggtacc gtgggagata tcgaatccct gcccttttta
     4321 gaggcgatca ggcagttcgg gttcgacaag gggaggggga acgtcctcta catacacctc
     4381 acgctcgtgc cgtacatcgc gaccgcgggc gagctcaaga cgaagcccac ccagcactcg
     4441 gtgcagaagc tcagggagat cggcatccag ccggcgatca tcctctgcag gtccgagcgc
     4501 aggctcacga gcgagctcaa ggagaagatc gccctgttct gcaacgtgga gaagagcgcg
     4561 gtcatcaacg cggtcgacgt ccagagcgtc tacgaggtcc ccatcgagtt ccacagggag
     4621 gggctcgacg accgcatcgt ggagtacctc aacatgtgga cccgcgagcc gcgcatgcag
     4681 ggatgggagg acctcgtcga gcgcatccgg aaaagaacgg agaaggtgaa catcgcggtg
     4741 gtcggcaagt acgtgaggct tacggactcg tacaagagcc tcaacgaggc cctcgagcat
     4801 gcgggcatcg c
//
LOCUS       DAOSCR010000134         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11140_length_4810_cov_2.759621, whole genome shotgun sequence.
ACCESSION   DAOSCR010000134 DAOSCR010000000
VERSION     DAOSCR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11140_length_4810_cov_2.759621"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLT69_04635"
     CDS             <1..773
                     /locus_tag="PLT69_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ13761.1"
                     /translation="IGSIAMFVFMANLFGTLVLLPIFLQNLMGYTATLAGIALMPGGV
                     ANLVTMPLVGRILHRYNPKAFVLAGIVVTAYSTLLMARFTLGVDFATVMVPRVVMGVG
                     MGLLFIPLTTLTIAHIEKEGMANATAIYNLIRNIGGSIGIAVVTTLLSRRSQFHQVRL
                     VEHLTPFDALYQVRTQQAASLLASKGFDPETARHAGLGLVYESMLKQAGMLSFADVFY
                     ALALMMVVLVPLVFFMKHTPHARQAEPPRGGTDAGHGA"
     gene            1635..2675
                     /locus_tag="PLT69_04640"
     CDS             1635..2675
                     /locus_tag="PLT69_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13762.1"
                     /translation="MRRPRRTDAIPVVLLSNMDPLWSEEDREEVRGLVRRLAEALSRA
                     GHPVSVEQLEREDLDALMAPYDPASCVVFNWCEGIPGIDHSEHLVARRLEELGFTFTG
                     ADSTVLETAQDKRLVKRILEGARVPTPRWRLYGAPRARDWDCFPAIVKAAHEHCSAGI
                     TPQSVVTTVEELEERIAYIVDAYRQPAIVEDFIDGREFHVSIWGNGALEMLPPAEMDF
                     SEFNDMHDRLCTRDSKCVPGSLHYEKIRTLLPAPLDAHEMRELERVCAAAYRAVGCRD
                     YGRLDVRVRDGVFYVLDVNPNADISIDASMACAAEVAGFPYHLMAGTIVRLACERLAS
                     GNTRARVVEDLI"
     gene            complement(2668..3099)
                     /locus_tag="PLT69_04645"
     CDS             complement(2668..3099)
                     /locus_tag="PLT69_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13763.1"
                     /translation="MSVGKAYTAFVESFSVKLGGGDSLENLNERIALVEKARMEALMI
                     SSDIRDPNEASFLSRYTDLLDRYLDLARRHAATLEEDASLRRQGIELEQKISSLKDAE
                     KNKATLEFNALWERHQELSKGRLAQERKELEELGRKLVSLK"
     gene            complement(3408..>4810)
                     /locus_tag="PLT69_04650"
     CDS             complement(3408..>4810)
                     /locus_tag="PLT69_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOJ13764.1"
                     /translation="SSTICFPPPAGASTTDPSAIERALSRIDLSSTLFVVSTKSGTTV
                     ETLSLFSYCYGLARKALGASRAGEHFVAVTDPGTPLVETARRCSFRRVFEGMPTIGGR
                     FSALSIFGLLPASLIGVDLATLLESATEQAQSCRAPELTLDCPNRAAVLGAALGVLAQ
                     AGRDKLTFFISEAASSFGDWVEQLVAESTGKQKTGILPVIGEAPGGVDLYGPDRVFCS
                     ISLKGEPPSSPEFGRLVDAGHPVIKIFIDDLHELGGLFYLWEFATAVAGHVLGINPFD
                     QPDVESTKKHTKAATEEYRLTGRLETGEPIIAQDGIEVYGGMKALTLRDALISFLFQA
                     REDSYLAIQAYLCPTSEVRDALDILRTLVRDRYRIATTLGFGPRFLHSTGQLHKGDGG
                     KGLFIQITADDRKDLPIPDDPLSDESSMGFAALKKAQWIGDARALQAAGRRVMRIHVT
                     LGDVAAAVRRISSALA"
BASE COUNT          818 a         1545 c         1604 g          843 t
ORIGIN      
        1 ccatcggcag catcgccatg ttcgtgttca tggcgaacct gttcgggacg ctcgtgctcc
       61 tgcccatctt tctccagaac ctcatggggt ataccgccac gctcgcaggc atcgcgctca
      121 tgcctggcgg tgtcgcgaac ctggtcacca tgccgctcgt gggcaggatc ctgcacagat
      181 acaacccgaa ggcgttcgtc cttgcaggta tcgtggtgac cgcatactcg acgctcctca
      241 tggccaggtt caccctgggg gttgacttcg ccaccgtcat ggtgccgagg gtggtcatgg
      301 gcgttggcat ggggctgctc ttcatcccgc tcaccacgct gaccatcgct cacatcgaga
      361 aggaagggat ggcgaacgcc acggcgatct acaacctgat acgcaacata ggcgggagca
      421 tcggcatcgc cgtggtgacg accctgttga gcaggcgctc ccagttccac caggtacggc
      481 tcgtggagca tctgaccccc ttcgacgccc tgtaccaggt gaggacgcaa caggctgcgt
      541 ccttgctcgc ttcgaagggg ttcgacccgg agacggcccg gcacgcaggc ctcggcctcg
      601 tctacgaatc catgctgaag caggcgggca tgctctcgtt cgcggacgtg ttctacgcct
      661 tggccttgat gatggtcgtc cttgtgcccc tcgtgttctt catgaagcat acgccccacg
      721 ccaggcaggc cgagcctccc aggggcggaa cggacgccgg ccacggcgcc taggcggacc
      781 cttgcgacct gtgggtgtac ctggcccgct tggggataca tctcgaaggc agggcacccg
      841 agggtgtggt ggccggttgg ccgtataccg gggggcctcg ggatgtggct gcgtactgcg
      901 gggagagaga agggacaagc tggcgcagtg accggggagg atgaaccagg ggagagacag
      961 ctcaacagac gcatccatca tgggaccttc cccttgcaag cggtgtccgc aatcgtggca
     1021 acccaccaca ggacccttga gggctctctc tccgtgcaac acaatgcgca tcagggactt
     1081 gatctcctcg aggtaatcgg tgacaatgga atcggtgatg aacggtcgaa cggaaaacga
     1141 ccagaagcag gccgcctgcg accacctcat ggcgcaagag gggggcatgc cgagcggtcg
     1201 ggagcgcccc tcctcgtcct ccaggctgac cgtcacccca cagcacccct cgtcgcagcc
     1261 cgatcgatgc attcgcgcct tcaggaaggc tgaacgcgaa gagaactccc gacgcccacg
     1321 agagatatcc gcctgctcga agcccgcctt gccccttgtg gccccacgcg tcctccgaac
     1381 agggctccct gctctgagaa gagcattctc ccggccccgc gcaccccggg gggactcgag
     1441 tgttccccgg ccgagcgcat cctccttggt ggcaggaaca gttgaagggt tgcttcgtgc
     1501 ggccccctcg agtgctcctt ggacaggcgc atcctccctt gaaggctttc cccatgggac
     1561 aagcacgcgg catgcccgcg cacgaacgtg catgatgccc ttcatggcat cgatcacggt
     1621 gcggaggtga gacggtgagg cgacccaggc gcacagacgc gatacccgtg gtgctcctgt
     1681 caaacatgga cccgctctgg agcgaagagg acagggagga ggtgaggggg ctcgtccggc
     1741 ggctggccga ggccctgtcc agggctggac accccgtgag cgtcgagcag ctcgagcgcg
     1801 aagacctcga tgcactcatg gccccctacg atcccgcctc gtgcgtggtg ttcaactggt
     1861 gcgaagggat ccccgggata gaccacagcg agcacctggt ggcccgcagg ctcgaggaac
     1921 tcggcttcac cttcaccggg gccgactcca cggtgctcga gacggcccag gacaagcgtc
     1981 tcgtgaagag gatcctcgag ggggcccgtg tgccgacgcc caggtggcgt ctctacgggg
     2041 ccccccgggc gcgcgactgg gactgtttcc cggccatcgt caaggcggcg cacgagcact
     2101 gcagcgcagg catcaccccg cagtccgtgg tcaccaccgt cgaggaactc gaagaacgca
     2161 tcgcctacat cgtcgatgcg taccgccagc ccgccatcgt ggaagacttc atagacggaa
     2221 gagagttcca cgtctccatc tggggcaacg gcgccctcga gatgctgccg cccgccgaga
     2281 tggacttctc cgagttcaac gacatgcacg acaggctgtg cacccgtgac tcgaagtgcg
     2341 tccccggttc cctccactac gagaagatca ggaccctcct gcccgcaccc ctggacgccc
     2401 acgagatgcg cgagctggag cgggtctgcg ctgcggccta cagggctgtg gggtgcaggg
     2461 attacgggag gctcgacgtg cgggtgcgcg acggggtctt ctacgtgctc gacgtgaacc
     2521 cgaacgccga catcagcatc gacgcgagca tggcctgcgc agcagaggtg gcgggtttcc
     2581 cctaccacct catggccggg accatcgtga ggctcgcctg cgagaggctc gcctcgggaa
     2641 acacacgcgc aagggtcgtc gaggatctca tttgagggag acgagcttgc ggcccagctc
     2701 ctcgagctcc ttgcgttcct gggcgagcct ccccttggaa agctcctggt gcctttccca
     2761 cagggcgttg aactcgaggg tcgccttgtt tttctcggcg tccttgagcg aggagatctt
     2821 ctgctcgagc tcgatcccct gcctgcggag ggacgcatct tcctcgagcg tggcggcatg
     2881 ccttctggcc aggtccaggt aacgatcgag caggtcggtg tacctcgaca ggaacgaggc
     2941 ctcgttggga tcccgtatgt ctgacgagat catgagggcc tccatcctgg ccttctccac
     3001 gagggcgatc ctctcgttga ggttctcgag gctgtcaccg ccgccgagct tcacggagaa
     3061 ggactccacg aaggccgtgt aggccttgcc tacagacatc gatcttacag gctccccggc
     3121 cggcccgggc gccgtacggt caggagttgc ggcctcgccc tgggccgacg gtgcggctgc
     3181 acgttccacg ccctgcccac cgtgcgtgtc cggcgtgccc tgtggttgcc ggtcatgccg
     3241 ctggcatccg tgcatcaaga ggatcgcggt gacgagtgcg aagaggagca atcgaagcgt
     3301 cgtcttgttc atgtgcacct ccctgcctga agaaggggct tcaggtatca ggctgattgg
     3361 atccatcgct caccctccct tccggatgaa cgtgtattcg tccgccgtca cgcaagggcc
     3421 gaggagattc tcctcactgc ggccgcaacg tcgcccaggg tgacgtgtat gcgcatgacg
     3481 cgcctgcctg cggcctggag tgcccgtgcg tcccctatcc actgggcctt cttgagggcg
     3541 gcgaaaccca tggacgattc atccgagagc gggtcgtcgg gtatggggag atctttccgg
     3601 tcgtcggcgg tgatctggat gaagagcccc ttgccgccgt cgcccttgtg gagctggccg
     3661 gtcgagtgga ggaacctggg gccgaacccg agggtggtcg ctatcctgta gcggtctctc
     3721 acgagggtgc ggagtatgtc cagggcgtct ctgacttcgc tggtcgggca gaggtacgcc
     3781 tgtatggcga ggtaggagtc ctcgcgcgcc tggaacaaga acgagatgag ggcgtcgcgc
     3841 agggtgagcg ccttcatgcc cccgtagacc tcgatgccgt cttgggcgat gatcggttcc
     3901 ccggtctcta gcctgccggt gaggcggtac tcctcggtgg ccgccttcgt gtgctttttc
     3961 gtggactcga cgtcgggctg gtcgaacggg ttgatgccca gcacgtgtcc ggcaacggcc
     4021 gtggcgaact cccagaggta gaacagcccg ccgagttcgt ggaggtcgtc gatgaagatc
     4081 ttgatcaccg ggtggccagc atccacgagc ctcccgaatt ccggcgacga gggaggctct
     4141 cccttgagcg agatggagca gaagactcga tccgggccgt agaggtcgac gcccccgggc
     4201 gcctcgccga tcacgggcag gatccccgtc ttctgcttgc cggtgctctc ggcgacgagc
     4261 tgttcgaccc agtccccgaa ggaggaagcg gcctccgaga tgaagaaggt cagcttgtcc
     4321 cggccggcct gggccagcac gcccagggcg gcgcccaaga ccgccgcgcg gttcgggcag
     4381 tcgagggtga gctcgggggc cctgcagctc tgggcctgct cggtggcgct ctccagcagg
     4441 gtcgcgagat cgacgccgat gagggatgcc ggcaggagcc cgaagatgga cagcgccgag
     4501 aacctgccgc ctatggtggg catgccctcg aagacccgcc tgaacgagca cctgcgcgcc
     4561 gtctcgacga gaggggtgcc ggggtcggtg acggcgacga agtgctcgcc cgccctggag
     4621 gcgcccaggg ccttcctcgc caggccgtag cagtacgaga agagggagag ggtctcgacg
     4681 gtggtcccgg atttcgtgga cacgacaaag agcgtggagg acaggtcgat cctcgagagg
     4741 gcccgctcga tggcggacgg gtcggtggtc gaggccccgg ccggcggcgg aaagcagatg
     4801 gtgctgctcc
//
LOCUS       DAOSCR010000135         4797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11178_length_4797_cov_3.907423, whole genome shotgun sequence.
ACCESSION   DAOSCR010000135 DAOSCR010000000
VERSION     DAOSCR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11178_length_4797_cov_3.907423"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLT69_04655"
     CDS             <1..203
                     /locus_tag="PLT69_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13765.1"
                     /translation="GHAPSADFILEFANSEVFLDVADDKVDVMARACLGDVSLKGDLH
                     MGQVLSRGLDVVGRYLLQETHS"
     gene            200..1435
                     /locus_tag="PLT69_04660"
     CDS             200..1435
                     /locus_tag="PLT69_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ13766.1"
                     /translation="MTYTFARSMELFSRAAKVIPQGIPGHLTPVFTVPGSYPYFCSHA
                     KGSRFVDVDGNEYIDYMCAYGPMILGYANEKVDEAYRVQAARGNCLSLANGLSVELAE
                     LIVDTVDCADWVMFAKNGADATSMAVMLARGYTGRDIVVLAEGGYHGTQSWAGTNHVG
                     VTARDRADIVMVPWGDPDALRRVVGRHKGSVAGVMLTPYHHPVFEDQRLPDPGFWPEV
                     RRICDEHGIVLILDDVRAGWRLDMKGSGHFFGIRPDLACYSKAMANGYPISALVGVER
                     LRITASKAYFAGSFWCNAPEMAAAIACIEEMKRIDAPRIVSELGAKLTNGLAERARAN
                     GLEVVISGPPAIPFMRFASEKNFLRSQLFCGECAKRGVFFHPHHNWFVSAAHTEADIE
                     RTLEVADVCFGIVKDRFGS"
     gene            complement(1921..2502)
                     /locus_tag="PLT69_04665"
     CDS             complement(1921..2502)
                     /locus_tag="PLT69_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13767.1"
                     /translation="MRHLQGPAFAVALAALVILPCRQASGACLEIPKDALLLIEKSRT
                     ESCALCARQFRKEAFAILDAALAPGTQIVPAGPCVFEKGASPDVQEFTLSPAPGEDGK
                     AEGGASEHAPEVVFVFHTRARRLVGIPERECTADEVAAAFRGLAPKSGFRGRARLVRF
                     AYGEGRTFTYLVKENRIVIHCRLEEVSPAKAGP"
     gene            complement(2499..3422)
                     /locus_tag="PLT69_04670"
     CDS             complement(2499..3422)
                     /locus_tag="PLT69_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ13768.1"
                     /translation="MERVLLSFKRTGRKVRVKEEPAASPGRGSPRWTVEGRAFPAAAL
                     FLSAFLACAGCVCRAVYVPDDVIHATPGMLGMPHEEASITTADGEVLGAWWIPARNPS
                     GTVLFCHGNGGNISTCLEAARLINSMGLNVLLFDYRGYGRSTGSPSEEGTYRDAAAAW
                     DYLVRVRGVRPETIVVWGKSLGGAVAARTAAEYKPAAVVIESAFTSIPDLVSDMGYPI
                     LSRCFSGCSHDTLAWVRRLEVPLLVIHSPQDEIVPFSHGRVLYDAAPLPKEFVAVDGS
                     HNTLSRASLPSYEASVRAFLSRHLGARGAGP"
     gene            complement(3404..>4797)
                     /locus_tag="PLT69_04675"
     CDS             complement(3404..>4797)
                     /locus_tag="PLT69_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOJ13769.1"
                     /translation="ANESALLSYSVSYSALTSETFGPAFALKSIFGFYPGYFSVLPYY
                     RKLQEYNDVDHRDIWEYRLDLTEEETRRMILHVRELQDIHSDYYFFDENCSYSLMYLL
                     DAARDGERLTGKVRGWMIPLDSIRMIERAGMIEGVSYRPSRVTRIRYFLGRMTDAERE
                     MAYSLATSDGDTHHDEGLTTGRGALVLEAAGEYLQYLYTKGAVSRDDYRRRYLKILTA
                     RSALGPQEDEERGEPAPGADPLKGHLSNRFSLGAGARTGDPFVEFRVRPAYHALMDDD
                     TGYVEGAHLVFADVILRSYPGEDRFVLQAIDAIDIVSLCPRDRFFRPISWKVKAGLTR
                     VPDSNGADRLVFGVNPGAGWAFGETAGLLCYGMVETAAYVGGVLEKDCLFGAGASAGV
                     LKTFPTPLKWKVHLFTRGLTLGPWDRHNHFEAGLSQGLTLGKDESLGIDLAFERERGF
                     PQWESKVSWNVFF"
BASE COUNT          850 a         1596 c         1543 g          808 t
ORIGIN      
        1 cgggacatgc gccctcggcc gatttcatcc tggagttcgc aaacagcgag gtgttcctcg
       61 acgtcgcgga cgacaaggtc gatgtcatgg cccgagcctg cctcggcgac gtttccctga
      121 agggagacct gcacatgggc caggtcttga gccgggggct ggacgtcgtc ggcagatacc
      181 ttctccagga gacccattcg tgacgtacac gttcgccaga tccatggaac tcttctcgag
      241 ggcggccaag gtcattcccc aggggatccc ggggcacctc acccccgtct tcacggtgcc
      301 tgggtcctac ccgtatttct gcagccacgc caaggggtcc cgcttcgtgg acgtggacgg
      361 aaacgagtac atcgactaca tgtgcgcata cggccccatg atcctgggat acgcgaacga
      421 gaaggtggac gaggcgtacc gggtccaggc cgcgcgcggc aactgccttt ccctggcgaa
      481 cggcctctcc gtggagctcg ccgagctcat cgtggacacc gtcgactgcg cggactgggt
      541 catgttcgcc aagaacgggg cggatgcgac gagcatggcg gtgatgctcg cccgcgggta
      601 cacagggcga gacatcgtgg tcctcgcgga gggtggctac cacgggacgc aaagctgggc
      661 cggcacgaac cacgtgggcg tgacggccag ggaccgcgcg gacatcgtca tggtcccctg
      721 gggcgatccc gacgccttga ggcgggtggt cggacggcac aagggttcgg tggcgggagt
      781 catgctgaca ccctaccacc acccggtatt cgaggaccag aggctgcccg accccggctt
      841 ctggccggag gtacgaagga tctgcgacga gcacgggatc gtcctcatcc tcgacgacgt
      901 gagggcaggc tggaggctgg acatgaaggg ttccggccac ttcttcggca tcaggccgga
      961 cctggcctgc tacagcaagg ccatggcgaa cgggtatccc atatcggccc tcgtgggtgt
     1021 cgagcgcctg aggatcaccg cctccaaggc ctacttcgcc ggctccttct ggtgcaatgc
     1081 accggagatg gccgcggcga tagcctgcat agaggagatg aagcggatcg atgctccgag
     1141 aatagtcagt gagctgggcg ccaagctgac gaacggcctc gccgagcgcg cccgggccaa
     1201 cggcctggag gtcgtgatat cgggaccgcc cgcgatcccc ttcatgaggt tcgcaagcga
     1261 gaagaacttc ctgcgctccc agctcttctg cggcgagtgc gccaagcgtg gcgtcttctt
     1321 ccacccgcac cacaactggt tcgtctccgc ggcccacacg gaggccgaca tcgaacgcac
     1381 gctcgaggtc gcggacgtgt gcttcgggat cgtcaaggat cggttcggga gctgaggaaa
     1441 ggaagtcgag gccgaagagg agacggccat ccccgcacga ccccgcctgc agcccgttgc
     1501 gggactgtct cgcgcgcacc ggccgaagag cggtgacgat ctgggtgcac cgccgccggc
     1561 cttctcgggc gggaccaggg gtgtgttgtg gcgggaaggt gtacgtctcg gcccgatgga
     1621 tccttccgtg gcggcgctcg cttggcatga agacgtccgt tttcgtgatc ccttgcatcc
     1681 accaaccccg gatacggccc tggaaggagg gtttgccgca cctcgggcgg cacggcgtcg
     1741 tcgccgggaa aggcgcccgg gcctctgcct gtacctgcaa tgaaggtcat tccgccgaag
     1801 gaccagccat gcaggccccc tgaggtgaga ccccgacggg tgacagggcc acccgcggcg
     1861 ccgacgaggg cataaataga tagccacgag agatcgcaaa gcctctgccg aaatcccgcc
     1921 tcagggccct gccttggcag gcgagacctc ctcgagcctg cagtggatca cgatacggtt
     1981 ttccttcacg aggtacgtga aggtcctgcc ttccccgtag gcgaacctca cgagcctcgc
     2041 cctccccctg aacccggact tcggcgcgag gcccctgaag gccgccgcca cctcgtccgc
     2101 ggtgcattcc cgctctggta tccccacgag ccttctcgct cgtgtgtgga agacgaacac
     2161 gacctcgggg gcgtgttccg acgcgccccc ttcggccttc ccgtcttcgc cgggggccgg
     2221 gctcagggtg aactcctgga cgtctggcga agcgcccttc tcgaagacgc agggtcctgc
     2281 aggcacgatc tgcgtccccg gcgcgagcgc ggcgtccagg atcgcgaagg cctcttttcg
     2341 gaactgcctg gcgcacaggg cgcaggactc ggtcctggat ttctcgatga gcaggagggc
     2401 gtctttgggt atctcgaggc acgccccact ggcctgccgg cagggaagga tgacgagcgc
     2461 cgcgagggcg actgcgaagg ccgggccctg gaggtgtctc atggccctgc cccccgagcg
     2521 ccgaggtgcc gcgacaaaaa cgcccgcacg gacgcctcgt aagaggggag ggacgccctg
     2581 gagagggtgt tgtgcgaacc atctacggcg acgaactcct tgggaagcgg agccgcatcg
     2641 tagaggaccc tgccgtgcga gaacggcacg atctcgtctt gggggctgtg tatgacgagc
     2701 agcgggactt ccagccgtct cacccacgcg agggtgtcat gggaacaccc ggagaaacac
     2761 cgcgacagga tgggataccc catgtcagac acgaggtcgg ggatggaggt gaaggccgac
     2821 tcgatcacca cggccgcagg tttgtactct gcggccgtcc tcgccgccac tgccccgccc
     2881 agggactttc cccagacgac gatcgtctcc ggcctcacgc ccctcaccct caccaggtag
     2941 tcccaggcgg cagcggcatc cctgtaggta ccctcctcag agggcgaacc ggtactcctg
     3001 ccataccccc ggtaatcgaa caggagtacg ttcaggccca tgctgttgat cagccgtgcc
     3061 gcctcgaggc aggtggagat gttcccgccg tttccatgac agaacaggac ggtccccgag
     3121 ggattgcgcg ccgggatcca ccacgcgccg aggacctccc cgtcggcggt cgttatggac
     3181 gcctcctcgt ggggcatccc gagcatcccg ggcgtcgcat ggatgacgtc gtccgggacg
     3241 tagaccgccc ggcagacgca cccggcacac gcaaggaagg ccgagagaaa gagggcggcc
     3301 gccgggaacg ctcggccctc gaccgtccag cgcggtgagc ctctccctgg tgaagccgcc
     3361 ggctcttcct tcacccgcac cttccggccc gtacgcttga agctcagaag aacacgttcc
     3421 acgacacctt cgactcccac tgcgggaaac ctctctccct ctcaaaggcc aggtctatcc
     3481 ccagcgattc gtctttcccc agggtgagac cctggctgag ccctgcctcg aagtggttgt
     3541 gcctgtccca gggacccagc gtcaagcccc tcgtgaacag gtgcaccttc cacttcaggg
     3601 gcgtggggaa ggtcttcagg accccggccg aggcaccggc cccgaacagg cagtccttct
     3661 cgaggacccc gcccacgtac gccgccgtct ccaccatgcc gtagcaaagg agccccgcag
     3721 tctccccgaa ggcccaaccg gcacccgggt tgacgccgaa cacgaggcga tccgcaccgt
     3781 tgctgtcggg cacacgggtg agacccgcct tcaccttcca ggaaataggc ctgaaaaacc
     3841 tgtccctcgg gcagagagac acgatgtcga tggcgtcgat tgcctggagg acgaacctgt
     3901 cctcgccggg gtacgaccgg aggatgacgt ccgcaaacac caggtgcgca ccctccacat
     3961 agcccgtgtc gtcgtccatg agggcgtggt acgcgggcct caccctgaac tcaacgaagg
     4021 ggtcaccggt tcttgcgccc gcaccgaggg agaacctgtt cgagaggtgt cccttcaggg
     4081 ggtcggcccc aggggcgggc tcacctcgtt cttcgtcttc ctgggggccc aaggcgctcc
     4141 gggccgtcag tatcttcagg tagcgcctcc tgtagtcgtc acgggatact gcccccttcg
     4201 tgtagaggta ctggaggtac tcccccgcgg cctcgagcac cagggcgccc cgtcccgtgg
     4261 tcaaaccctc gtcgtgatgc gtatccccgt cggaggtggc caacgagtat gccatttctc
     4321 gttccgcatc cgtcatccgc cccaggaaat accttatcct cgtgaccctc gacggcctgt
     4381 aggagacccc ctcgatcatg cccgcgcgct cgatcatccg aatggagtcc agcggtatca
     4441 tccatccccg gaccttgccg gtcagcctct cgccgtcccg ggcggcgtcg aggagataca
     4501 tgagcgagta ggaacagttc tcgtcgaaga agtagtagtc cgagtgtatg tcctggagct
     4561 cccggacatg gaggatcatc ctcctggtct cctcttccgt gagatcgagc ctgtattccc
     4621 agatgtcccg gtgatccacg tcgttgtatt cctggagctt ccggtaatac gggaggacgg
     4681 agaagtagcc gggatagaac ccgaagatgc tcttgagcgc gaatgccggc ccgaaggtct
     4741 cggaggtgag agccgagtag ctcacggaat aggagagcag tgcgctctcg ttggcgg
//
LOCUS       DAOSCR010000136         4793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11188_length_4793_cov_3.506543, whole genome shotgun sequence.
ACCESSION   DAOSCR010000136 DAOSCR010000000
VERSION     DAOSCR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11188_length_4793_cov_3.506543"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..748)
                     /locus_tag="PLT69_04680"
     CDS             complement(2..748)
                     /locus_tag="PLT69_04680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ13770.1"
                     /translation="MDIPRIFTITESGHRIHNPFTPEKLAQLGEALRLEKGARVLDLG
                     SGSGEMLCTWARDYGIIGTGIDMSLLFTEQARRRAEELGVAHRVRFIHGDAAGFVSDE
                     PVDVAACVGATWIGGGVAGTIELLAKSVRPGGVILVGEPYWRRLPPTEDVARGCLAGA
                     ISNFLTLPDLLASFGSLGYDVVEMVLADQDSWDRYEAAQWLTMRRWLDANPDDEMAGE
                     VRARLTSAPVRYATYTREYLGWGVFALMKR"
     gene            complement(867..2324)
                     /gene="ppcA"
                     /locus_tag="PLT69_04685"
     CDS             complement(867..2324)
                     /gene="ppcA"
                     /locus_tag="PLT69_04685"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOJ13771.1"
                     /translation="MKIPHCMSTQHPDNASTPFFASSSVLAGEDEIREAYYAFSHLGC
                     DEQMWDVEGKEIDTYVVKKLLTYYPEYFHENVLGEDLRITLRVPNPTVEKAEAKILLE
                     TLESIPRSYDAARLFYANDVPPIFEVILPMTTSARCIDRVYHYYADHIIGRQNTRFCD
                     DDVTIAEWIGEFLPSRIQVIPLFEDVQSMLNADRIVEDYLSDKDVEDQRVFLARSDTA
                     MNFGLVSAALANKIALMNLDRLAERIGVRIHPILGMGSAPFRGGLSPRTAERVGREYP
                     SVVTFTIQSAFKFDYPVDEVRAACEHLKSRVIGHAAPIDVERAQSIMERYSKVYRRSI
                     AELAPHINRMAKHVPARRARKLHVGLFGYARELDGITLPRAISFTCSLYALGLPPELI
                     AFDALSDDDLSFIHDAYPSFRAKIEEALKFADLEGPLMTDSLREALGRFVGEPQTHEE
                     HLSVVRRIRNDLESGNTQQIGNHILSTALMRRFLG"
     gene            complement(2927..3520)
                     /locus_tag="PLT69_04690"
     CDS             complement(2927..3520)
                     /locus_tag="PLT69_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ13772.1"
                     /translation="MNDNAFMYRIDSTDTIVGISDNWCAFAYANAWRSSLRPEEIVGR
                     KLWDFIQGLETKYLYRELYRRAREGATLRPIPFRCDSPQERRHLELLIEALPDGQIEI
                     TSRICRTEPRSPVRLLDAAAPRSADLVTVCSMCKKIQVSPGEWAEIEDALARLALFEA
                     DTMPQLTHGLCRPCYRIAMGELDALKAPAKAVESDRE"
     gene            complement(3588..4502)
                     /locus_tag="PLT69_04695"
     CDS             complement(3588..4502)
                     /locus_tag="PLT69_04695"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ13773.1"
                     /translation="MDLQDRFRGCLLGLAVGDAVGTTVEFRPRGSFEPLTDMIGGGPF
                     NLLPGQWTDDTSMALCLATSLVECRGFDARDQMKRYCMWADTGYLSSTGECFDIGSTV
                     AAALRSFRRSGDPHAGPDDPHSAGNGSIMRLAPIPMFFFPDVDSAERFAAESSRTTHG
                     AAECIDACRLLARIICRALSGKPKEEVALGDGKTFAGSEKIAAIARGEYRGKSREDIR
                     GSGYVVDCLEAAMWCFVHTDGFAEAVLMSANLGDDADTTAAVCGQVAGAYYGSRGIPA
                     GWVERLAFGSDITKLADLLYGVSKERTG"
BASE COUNT          915 a         1466 c         1442 g          970 t
ORIGIN      
        1 atcaccgctt catgagcgcg aacacgcccc agcccaggta ttcccgcgtg tacgtggcgt
       61 agcgcacggg cgccgatgtc agccgggcgc gaacctcccc ggccatttcg tcatcggggt
      121 ttgcatcgag ccatcggcgc atggtgagcc attgggcggc ctcatacctg tcccagctgt
      181 cttggtcggc aaggaccatt tccacgacgt catagccgag gcttccgaac gacgcgagca
      241 ggtcaggaag cgtgagaaag ttggagatcg cgcctgcaag acaccccctg gcgacgtctt
      301 ccgtcggcgg caagcgccgc cagtagggct cgccgacgag gatgacaccc ccggggcgca
      361 cgcttttcgc cagaagctcg atggtgccgg caacaccccc tccgatccag gtggctccga
      421 cacatgccgc cacatcgacc ggctcgtcgg agacgaagcc ggccgcatcg ccgtggatga
      481 acctcacccg gtgggcgacc ccgagttctt ctgcgcgtcg tcgtgcctgc tcggtgaaca
      541 acaggctcat gtcgatgccg gtgccgatga tcccgtaatc gcgcgcccag gtgcacagca
      601 tctcccccga cccgctgccg aggtcgagca ccctcgcccc cttctccagg cggagcgcct
      661 ctccgagctg tgcgagcttt tcgggcgtga acgggttgtg gatgcggtgg ccgctttcgg
      721 ttatggtgaa tatcctggga atgtccaaaa cggcagatct cctttcacgt gtgaatacac
      781 acggtcacag cctgtcttcc atgacatgag cggccatcga cgtgatcccg ctcggtctca
      841 tggtcagccc tgcgtcggtg cgttgtctag cccaggaatc ggcgcatcag cgccgtagag
      901 agaatatggt tgccgatctg ttgcgtattc ccgctttcga ggtcgtttct gattctgcgc
      961 acgactgaca ggtgttcttc gtgggtctgg ggttccccta cgaaccttcc cagtgcctca
     1021 cgcagcgaat cagtcatcaa cggcccttcg agatcggcga acttcaaggc ttcctcgatc
     1081 ttcgcacgga acgaaggata tgcatcgtgg ataaaactga ggtcgtcgtc gctgagggcg
     1141 tcgaacgcga tgagctccgg cggcaggccg agagcgtaca gagagcacgt gaacgagatc
     1201 gctctcggca gggtgatccc gtcgagctcg cgcgcatacc cgaacaggcc cacgtgcagc
     1261 ttacgcgccc gccgcgccgg gacgtgcttt gccatccggt tgatgtgcgg tgccagctca
     1321 gcgatgctgc gtcggtagac cttgctgtag cgctccatga tcgactgggc tcgttccacg
     1381 tcgatgggtg ctgcatgacc gatgactcgc gacttgaggt gttcacaagc ggctcgcacc
     1441 tcgtcgaccg ggtagtcgaa cttgaacgca gactggatcg taaacgttac cacgcttggg
     1501 tactcgcgac cgacgcgctc tgcggtgcgt ggagagagtc ctccacggaa tggcgcagac
     1561 cccataccga ggatggggtg gatccgcact ccgatgcgct cggcgagacg gtcgagattc
     1621 atgagggcga tcttgttggc aagcgccgcc gagacgagcc cgaagttcat cgccgtatcc
     1681 gaacgcgcga ggaagactcg ctggtcctcc acgtccttgt ccgagaggta gtcctcgacg
     1741 atacggtctg cgttcagcat gctctggacg tcttcgaaca gcgggatgac ctggatacgg
     1801 gaaggcaaga actcgccgat ccactcggca atggtgacgt catcgtcgca aaagcgcgtg
     1861 ttttgccggc cgatgatgtg atcggcatag tagtgataga cacggtcgat gcagcgtgcc
     1921 gaggtcgtca tgggcaggat gacctcgaag atcggcggga cgtcgttcgc gtagaacagg
     1981 cgtgccgcgt cgtacgagcg cgggatgctc tcgagcgtct cgagcaggat cttcgcctcg
     2041 gccttctcga cagtcggatt cgggacccgc agcgtgatcc tcaggtcttc accgagcacg
     2101 ttctcgtgga agtactcagg gtagtatgtc agcagcttct tgacgacgta ggtgtctatt
     2161 tcttttcctt cgacgtccca catctgctcg tcgcatccca gatgggagaa ggcatagtag
     2221 gcctctctta tctcgtcttc gcccgcgaga accgatgacg acgcaaagaa aggggtcgag
     2281 gcgttgtccg gatgctgtgt gctcatacag tgcggtattt tcatagacag ttccgtcctc
     2341 ccaagctgac tatgcgctct tcgggttcac gaccgagatc gttcgttgtt accacagcgg
     2401 ggtaactaat tcaaagtatt atcaggctcg atgctgtaca agagcagacc tgccgcaaag
     2461 ggctgtcgtg acgagacccc atgggatcta taccgatcat gcttctcatg ttgctttcat
     2521 gggaacgaac ccgtgagtga cggccgagct ttcaacgagg ccggtcggtg ttattacccc
     2581 tgacccgatc tttcatgcct ctctttgctt cactgaatga agagaaggaa cggggtcgga
     2641 tgcattcctc cttccatggt ccgcatggac cgcacctcaa ccctgtgctg ccccggggta
     2701 tacggcgtga tgtcccccgt ttccatgcaa ggggcggccg tcaaccgcac accgctttcg
     2761 aggaaaggat cgagccgttg tctctttaga ggatgaccac tcggtgcagg ccctttcccc
     2821 aagaaagccg tgagacaagg cgtcctcgag ggcaccgact gccaccttgg cattcaggtt
     2881 cctgcaggga gggcactgta ctatgttatt ttcatgatgc ggcgcgctat tcccggtcgc
     2941 tctcgacggc cttggcgggt gccttgaggg cgtccagctc acccatcgca atccggtaac
     3001 atggccggca caaaccgtga gtcaactggg gcatggtgtc ggcctcgaag agcgcaagac
     3061 gggccagggc gtcttcgatc tcggcccatt cccccggtga gacctgtatc ttcttgcaca
     3121 tgctgcatac cgtgacgaga tcggccgatc tcggcgcggc cgcgtcgagc agacgaacgg
     3181 ggctgcgggg ctctgtccgg catatcctgc tggtgatctc gatctgtccg tccggcagag
     3241 cctcgataag gagttcgagg tgtcttcgtt cctgtggaga gtcgcatcga aaggggatcg
     3301 gccgcaacgt ggcgccttct cttgcccgtc ggtacaactc gcgatatagg tacttcgtct
     3361 caaggccttg aatgaagtcc cagagcttgc gcccaactat ttcttccggc cgcagggaac
     3421 ttcgccaggc gttcgcatac gcgaaggcac accaattgtc gctgatgccg acgatggtat
     3481 cggtgctgtc gatccggtac atgaacgcgt tgtcgttcat gtgctttgcc ctccaggcct
     3541 gacggaaccg agaccggccg tcgttggccc tcgcctgcag cgggttctca gcccgttcgt
     3601 tccttactca caccatacag caggtcagcc agcttcgtga tgtccgaacc aaacgcgagg
     3661 cgttctaccc agccggcagg gattcctcgg gagccgtaat atgcccctgc gacctgcccg
     3721 cacaccgcag cagtggtgtc ggcgtcgtcg cccaggttgg cggacatgag gaccgcctct
     3781 gcaaaaccgt cggtatggac gaagcaccac atcgccgcct ccaggcagtc taccacatac
     3841 ccggagccgc ggatatcctc tctcgacttt ccccgatact ctccacgagc gatggccgcg
     3901 atcttctccg accccgcgaa cgtcttgccg tcgcccagcg cgacctcttc tttcggcttg
     3961 ccggaaagcg cccggcaaat gatccgcgcg agcagacggc atgcatcgat gcactctgca
     4021 gcgccatggg tggttcggga gctctcagcc gcaaaccgtt ccgccgagtc cacatccggg
     4081 aagaagaaca tcgggatcgg ggccagcctc atgatgcttc catttccggc cgaatgcggg
     4141 tcatccggcc cggcatgcgg atcgccactc ctgcgaaaac tcctcagcgc ggcggccacc
     4201 gtactgccga tatcgaaaca ctccccggtg ctgctcaggt agccggtgtc ggcccacatg
     4261 caataacgct tcatttggtc acgcgcatcg aaaccgcggc actcgacgag actggtggcc
     4321 aggcaaagcg ccatggaggt gtcatcggtc cactggcccg gaaggaggtt gaagggaccg
     4381 ccgccgatca tgtccgtcag cggctcaaag ctcccccggg gccgaaactc gacagtggtg
     4441 ccgacagcgt ctcccacggc tagaccgagg agacacccac gaaaccggtc ttgaagatcc
     4501 atgcgtaccc tcccatacga gctgaccgcc agcatttgcc gtaggtcctg tctctatatt
     4561 tctataaaaa aggtggaagc gagccaaggg cacctccgat aataggcata gcgaccaact
     4621 aacgcttatt caggaggtgc gaatggctcg tggaggagat gttaaggggt tggtgggcat
     4681 atggcaagag gcgaccggtg agggggaaga cgggctgtta aggttgatgg agcatggtgt
     4741 gcagcgggtg ctggaagaag agctgacggc ataccttaag gcaaagcctc acg
//
LOCUS       DAOSCR010000137         4784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11217_length_4784_cov_2.619158, whole genome shotgun sequence.
ACCESSION   DAOSCR010000137 DAOSCR010000000
VERSION     DAOSCR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11217_length_4784_cov_2.619158"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLT69_04700"
     CDS             <1..328
                     /locus_tag="PLT69_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ13774.1"
                     /translation="MEFDTMVRHNIPVLVIVNNDQAWGMIKHSQELSIGCDRLQCAEL
                     GERRYEKMVEGLGGHGEFVTRDEEIVPAIRRALESGRPACVNVMTDPTATSPATPLFY
                     QALKME"
     gene            332..1546
                     /locus_tag="PLT69_04705"
     CDS             332..1546
                     /locus_tag="PLT69_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ13775.1"
                     /translation="MKALVFSITVPQYLVLQVLGRLNRKYFFEGPFATVSLRDVPEPA
                     LPGREWVKVRVRLCGLCGSDINLLMVKDSPMASPFTSFPCIFGHELAGEVVETGADVH
                     DCKVGDLVTVNPSLSCVTRGLTPVCRMCSSGRPGNCERFAEGAFAPGMFAGICKDIGG
                     GFAEYMVAHTSQVFRVPDGVSPESAVLTEPLAVGLQAVLDNLPKDGDAVLVIGGGVIG
                     AMVVKAIRGLGIGCSVTVVEPSAFHREYARRSGADHVVASGILDAAVKMAGARAYKPM
                     LGEHILQGGFDKVFDTVGHSDTIQQALIATSGLGTVSLVGIGNKVTFDPTPLWLKLLT
                     LKGCYGHAVNEDGSGKRHAFSRALELVASSRVHVEDMLTHTFPITRYRELLEVSVSKG
                     SYQAIKTAVRFF"
     gene            2477..3955
                     /locus_tag="PLT69_04710"
     CDS             2477..3955
                     /locus_tag="PLT69_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOJ13776.1"
                     /translation="MSDTFFTFLAGFRIQDFFDIAIISFLLYLAIVWFKHATSKLVLV
                     GMILLGVVYIGARLFHLYLTTIVLQSFFTVIIIALVVIFQEDIRRFFERLATLRNLAR
                     HRRKSAEGVDGAVSAIVESSAELASRRIGALIVIEGEDPIDRQITGGYTLNGVLTSPL
                     VMSIFDPHSQGHDGALVVRNGRAFKFGCHLPLSTNTEKLANFGLRHTAALGLSERCDA
                     LAIVISEEKGTISVVRNAEIVTLKNAIELNSVLEQFFEKTFTDGGKHAKGGLGKNALE
                     KGLALVLAVGLWFVFGYQRESIQRDYIVPIEYRKISPEWEIEEARGTDATVTLMGSTQ
                     AFRLFDPSSLKISIDLSSLEEGRQVVVITSDMVNVPSNLTLLKVRPDRIIITAHRLFP
                     RDVKVTPDMVGRPEEGYEIRQVTVEPEFVSVLAPFAITASRISVITEPIDVSGITSSH
                     TYTVKVIPSTSRVRFKAQQQPTVRVTVEVERTSRRDAPKKGT"
     gene            complement(4013..4273)
                     /locus_tag="PLT69_04715"
     CDS             complement(4013..4273)
                     /locus_tag="PLT69_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HOJ13777.1"
                     /translation="MAGEKKLIGKVTHFFGKISVAGIQLTDTLKVGDRISIEGATTSF
                     EETIDSMQIDNKPVDVAGPGDLIGIKVKEKTRVGDSVYLITD"
     gene            4470..>4784
                     /locus_tag="PLT69_04720"
     CDS             4470..>4784
                     /locus_tag="PLT69_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13778.1"
                     /translation="MSYRFDTLCGLFHNQAQRYGEDHVFLSGAFDEHGHPMDTFRNIT
                     WAQARRLALDLARGLAALGVKPGDRCVIFSESRPEWVIADQAVQACGAVGVPLYPTLN
                     PVE"
BASE COUNT          843 a         1496 c         1566 g          879 t
ORIGIN      
        1 catggagttc gataccatgg tgaggcacaa catcccggtg ctcgtcatcg tgaacaacga
       61 ccaggcctgg ggcatgatca agcacagcca ggagctgtcc ataggctgcg accgcctcca
      121 gtgcgccgaa ctgggagaga ggcggtacga gaagatggtc gaggggctcg ggggccacgg
      181 cgagttcgtg acgagggacg aggagatcgt gccggccatc aggcgcgccc tcgagtcagg
      241 caggcccgcc tgcgtaaacg tcatgaccga cccgactgcc acgagcccgg cgacacccct
      301 gttctaccag gccctcaaga tggagtgagc cgtgaaggcg ctggtctttt ccataacggt
      361 accccagtac ctcgtgctcc aggttctcgg caggctcaac aggaagtatt tcttcgaggg
      421 cccgttcgcc acggtgagcc tcagggacgt gcccgagccg gccctgccgg ggcgcgagtg
      481 ggtgaaggtc agggtgaggc tctgcggtct ctgcggcagc gacatcaacc tcctcatggt
      541 gaaggattcc cccatggcct cccccttcac ctccttcccg tgcatcttcg ggcacgagct
      601 ggcgggcgag gtggtggaga cgggcgcaga cgttcacgac tgcaaggtgg gcgacctcgt
      661 cacggtcaac ccgtccttga gctgcgtcac gagagggctc acgcccgtct gccgcatgtg
      721 ctcctcgggc aggcccggca actgcgagcg gttcgccgag ggggcgttcg ctcccggcat
      781 gttcgccggc atatgcaagg acataggcgg gggcttcgct gaatacatgg tcgcccacac
      841 atcccaggtg ttcagggtgc cggacggggt ttcccccgaa tccgcggtgc tgaccgaacc
      901 tctcgccgtg gggctccagg cggtgctcga caacctgccg aaagacggcg atgcggtcct
      961 tgtcatcggc ggcggggtca tcggcgccat ggtggtcaag gccatacgcg gcctcggcat
     1021 cggctgcagc gtgaccgtgg ttgagccgtc ggcctttcac cgcgaatacg ccaggcgctc
     1081 gggagccgac cacgtggtcg catccggcat cctggatgca gccgtcaaga tggccggcgc
     1141 acgggcctac aagcccatgc tcggggagca tatcctccag ggcggcttcg acaaggtgtt
     1201 cgacacggtc ggccactcgg acacgatcca gcaggccctc atcgcgacct caggtctcgg
     1261 cacggtgagc ctggtgggca tcggcaacaa ggtgaccttc gaccccacgc cgctgtggct
     1321 caagctcctc accctcaagg ggtgctacgg gcacgccgtg aacgaggacg gatcggggaa
     1381 gaggcacgcc ttctcccggg ctctcgagct cgtggcctcc tcgagggtcc acgtggagga
     1441 catgctcacc cacaccttcc ccataacgag gtacagggaa ctcctggagg tgagcgtgtc
     1501 caaggggtcg tatcaggcga tcaagaccgc ggtgcgcttc ttctgagaac cacccccgtc
     1561 atcggtctcg ccggggagtc ggccctttcc ctggaggccg cccctcgggg gacgggcttg
     1621 ctctttcggc ggcctcgagg tgagggccgt cccttcgagc gaagagaggg ggatgttctc
     1681 tgatggaacc gtggcggtgc ccccctgtcg acaggcgttg ggggggcatc gggccagggc
     1741 cgagcacttc gtccttgcag ggccgttcgt acgccgcgaa gcctcggctc tcccccgtga
     1801 cgccggtaca tccacgtcat ggggtctcgg ggttcctcag ggagacaccc gccgacgaga
     1861 cgggcacgcc cccaggagga agaggcctct tagacgtccg tgtttagggt cgtccagctc
     1921 gacgcatgca cggcaggacg tgcatgaccc ccgtcccggt ataccgtcgg gccaggtgct
     1981 ccattctctc gccatgtggg gagtatgacc ctcgtccggg tatgccggct gtgctttcgc
     2041 gtgcgggctt gtcctgaggg gtgtcttccg gcgggacgct tctctctggg cgcgtccgcc
     2101 ttgccacgtg cttggaggaa tgccgccttt ctcggcatgg atggagggtg agggcggccc
     2161 tgtccgactt gccgaggagg tcgggatgga tccttcgggc gcgcgggctt ccttgcctgt
     2221 tcacgagagg gggccctgcc cggccgcacg ggatggggcc ttttaaaccc gctctcgggg
     2281 cgcgggacac catgagcagg cggaaagatg acgccgcatg gagtgacggc ctgtccgtcg
     2341 agcggtcatg cccggcggaa ccgtgaagaa acctgcccct tgcgaacctg caaggatcgg
     2401 gataatctta aacccggccg cttgaggttg gtgccgcgtt cgtttcccgt tcagatgctg
     2461 gagggttgaa ggtcagatga gcgatacgtt cttcacgttt ctcgccggct tcaggatcca
     2521 ggactttttc gacatcgcga tcatctcctt cctcctgtac ctcgccatcg tctggttcaa
     2581 gcacgccacg tccaagctcg tgctcgtggg gatgatactg ctcggcgtgg tctacatcgg
     2641 cgcacggctc ttccacctct acctcaccac gatcgtgctc cagagcttct tcacggtgat
     2701 catcatcgct ctggtcgtga tcttccagga agacatcagg aggttcttcg agcgcctggc
     2761 caccctcagg aacctcgcca ggcacaggag aaagtccgcc gaaggcgtgg acggggcggt
     2821 gagcgccatc gtcgagagct cggcggagct cgcctcgagg cgcatagggg cgttgatcgt
     2881 gatcgagggg gaggacccca tagacaggca gatcaccggc ggatacaccc tgaacggcgt
     2941 gctcacctcg cccttggtca tgagcatatt cgatccgcat tcccaggggc acgacggtgc
     3001 actcgtggtc cgcaacgggc gtgccttcaa gttcggctgc cacctcccgc tgtcgaccaa
     3061 cacggagaag ctcgccaact tcggcctccg ccacacggcg gcactggggt tgtcagagcg
     3121 gtgcgacgcc ctcgcgatcg tcatatcgga ggaaaagggc accatctcgg tcgtgcgcaa
     3181 cgccgagatc gtgaccctca agaacgccat agagctcaac tcggtcctcg agcagttctt
     3241 cgagaagacc tttaccgacg gcgggaaaca cgccaagggc ggactcggga aaaacgccct
     3301 cgagaagggg ctcgccctcg tactggctgt ggggctctgg ttcgtgttcg gctaccagcg
     3361 tgaatccatc cagcgggact acatcgtccc catcgagtac cgcaagatct cgccggagtg
     3421 ggagatagag gaggcccggg gaacggacgc cacggtcacg ctcatggggt cgacgcaggc
     3481 cttcaggctg ttcgacccgt cgagcctcaa gatatcgatc gacctctctt cgctcgaaga
     3541 gggcaggcag gtggtggtca tcacctcgga catggtcaac gtgccgtcga acctgacgct
     3601 cctcaaggtc aggccggaca ggatcatcat caccgcgcac aggctcttcc cacgcgatgt
     3661 gaaggtgacg ccggacatgg tcggcaggcc cgaagaggga tacgagatca ggcaggtcac
     3721 cgtggagccg gagttcgtct cggtgcttgc gccgttcgcc ataaccgcga gcaggatctc
     3781 ggtgatcacc gagccgatcg atgtctcggg catcacctcc agccacacct acaccgtgaa
     3841 ggtgataccg tccacgtccc gggtgagatt caaggcccaa cagcaaccga ccgtccgcgt
     3901 gacggtggag gtggaacgca catcccggag agacgcaccg aagaagggca cgtgacggca
     3961 catcgggccg ccctccgtga tgccggcccg gggtgccccg ggtgagccgg gatcagtcgg
     4021 tgatcaggta aacactgtca ccgacgcgtg tcttctcctt gaccttgatg ccgatgaggt
     4081 ctcccggccc ggcaacgtct accggcttgt tgtcgatctg catggagtcg atggtctcct
     4141 cgaacgaggt ggtcgccccc tcgatggaga tcctgtcgcc gaccttgagg gtgtcggtca
     4201 gctgaatgcc cgcgacgctt atctttccga agaagtgggt gaccttgccg atgagtttct
     4261 tttcgccagc cattgtgaat acctcctcga atgtatgtct ttataacccc tctgcaggga
     4321 aatttcaagg gaatcggcgc gggtgcggcc cacagggaga gggagagagg cttggacccg
     4381 gcctcgggct tctcaaaccc cttgagatca ggtacaatca tggaagagag cagccggcgg
     4441 cttcgatcat ctttctctgg gggaatgtga tgtcgtacag gttcgatacc ctctgcggac
     4501 tgtttcacaa ccaggcacaa cgatacggcg aggatcatgt ctttctctcg ggcgcgttcg
     4561 acgagcatgg gcatcccatg gacacgttcc ggaacataac gtgggcccag gcgaggaggc
     4621 tggcgctcga cctggccagg ggactggccg ccctcggggt gaagcccggt gaccggtgcg
     4681 tcatattctc ggagagcagg ccggagtggg tcatcgcgga ccaggccgtg caggcgtgcg
     4741 gtgccgtcgg ggtgccgctg tacccgacgc tcaatcccgt cgaa
//
LOCUS       DAOSCR010000138         4756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11311_length_4756_cov_3.098490, whole genome shotgun sequence.
ACCESSION   DAOSCR010000138 DAOSCR010000000
VERSION     DAOSCR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11311_length_4756_cov_3.098490"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLT69_04725"
     CDS             complement(<1..209)
                     /locus_tag="PLT69_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOJ13779.1"
                     /translation="MAETKGTRVSAVWGFEARTRLEIPLFTARVQAGFPSPADDFVDK
                     RLDLNELLVAHPAATFFVRVEGTSM"
     gene            744..1970
                     /locus_tag="PLT69_04730"
     CDS             744..1970
                     /locus_tag="PLT69_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOJ13780.1"
                     /translation="MGRESDREYRRRVSGYYRQLMERCQAHMDMLEADLPPAREGLGE
                     VLEERGVTRRDFLKWTSAMTAALMLPPMFRPLVARAAENFGRLPVVWLHFAECTGCSE
                     AFLRSSYPNVDDILLETISLEYHETLMAAAGKQAEECLEKALADFPGKFVCVIEGALP
                     TGLDGQYLRLGPKGKTGIEIAKEVTSKAAATICIGSCSSFGNVQAAKPNPTDAKGISE
                     AIGIQTVNISGCPPSPQNFVGTVLHFLMFGALPPVDNLGRPVWAYGKRIHDYCERRPH
                     YDAGEYVEEWGDEGAKKGWCLYKMGCKGPYTYSNCAKVRFNDGVSWPIMAGHGCMGCT
                     EPGFWDTMAPLEKPLTDKPVGGWGVTARIDKLGLGLGAATLVGIGAHALVTGALKHGQ
                     PAKPESDEKTDQGSHE"
     gene            1997..3670
                     /locus_tag="PLT69_04735"
     CDS             1997..3670
                     /locus_tag="PLT69_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOJ13781.1"
                     /translation="MAQRIVIDPITRIEGHLRIEVELDDTNTVKDAWSSITLWRGIKT
                     ILKGRDPRDAPLMVQRFCGVCTYVHYEASILALEDAFGVRPPANARLVRNLIHAALYM
                     TDHIMHFYHLHGLDWVDITKAISADPAKAVELAKQYSDNPYNCSETHYRAVQKRLAKY
                     VESGRLGPFANAYWGNPSYKLSPEANLIIVSHYLDALQVSKTGAQMMAIFGGKNPHPQ
                     TLVAGGITSVMDMLDAHRIAEYQFRLNELKRFIDTAYLPDLLLAAGAYKDEGLAGVGA
                     GVKNYICYGGFPLDDQWNKTLLPKGVIVGGDISKPLPLDEAKITEDVTHSWYKGDKPA
                     HPYDGSTEPEYTGLDANGYVKGNEKYSWCKGPCYDGKPFEVGPLARMVLGYAQGDERI
                     KPLVEDTLKATGLPATVLFSTLGRTAARGLETKLIADYTQNWINELVANIKAGDTRTW
                     TRCDVPKSGKGRGMVEVPRGALGHWVVIEDHVIANYQAVVPSTWNCAPRNQDGMRGPY
                     EESLIGTKLANPEQPLEILRTIHSFDPCMACAVHIMRPGSDEIMHFRVV"
     gene            3692..4360
                     /gene="cybH"
                     /locus_tag="PLT69_04740"
     CDS             3692..4360
                     /gene="cybH"
                     /locus_tag="PLT69_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HOJ13782.1"
                     /translation="MDTRTAKKEWSLSLRANHWIMALCIFGLIATGFYIARPITFSAG
                     ETIDKFAMGYARYFHLMFGVTLTFLFVWRVYLMFFSRFHADWKDFFAWTDFRNFWTQI
                     KFYTFLSQKKPEHTHLYGPLQSIAYGGFFAMLLVLIVTGLILAGVDYSSGFIGTAYAV
                     FNPITNAIGGLAVVRVVHHVFTWLVILFVVVHVYMAFWYDMVFKEGTISSMIGGRIFR
                     KQHE"
     gene            4565..>4756
                     /locus_tag="PLT69_04745"
     CDS             4565..>4756
                     /locus_tag="PLT69_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOJ13783.1"
                     /translation="MIQGEKKITVLGLGNILLKDEGFGVHFVRWFSERHSYGPEVELL
                     DGGTLGYRLLDIVTSTRHLI"
BASE COUNT          943 a         1449 c         1576 g          788 t
ORIGIN      
        1 atcatggacg tgccttccac ccgcacgaag aaggtcgccg cggggtgcgc gacgagcagc
       61 tcgttgaggt cgagcctctt gtccacgaag tcgtccgcgg gcgaggggaa gccggcctgg
      121 acccgggccg tgaacagggg gatctcgagc ctcgtcctcg cctcgaaccc ccagacggcg
      181 ctgacgcgtg ttccctttgt ctcagccacg gcaaaccatt gtaccaccag gggggtgacg
      241 tgtaaaggaa cgggcccggt ggtgcagggg acggcgggcc gaaagacggc cgagtcctcg
      301 ggccgcacgc ggtgtccggt catgaagggg tttactcccg ccgcctgtca cgtcccgggc
      361 cggcagcggc ggccccggcc catgcggtgg aggttgttca gggagccgtg cgtcaccggg
      421 gccggccggc aggctgggta cggcgcactg cccgtggtgc atcgaggggc ctgtgcggtc
      481 gggccgtctc aagacccccg ggcggggtcc tgcagggtga aaagcatctt gacgagaccg
      541 ggttctcttc tgtatttcat ctcgctaatc ccctgcgaac gcctcgtagg agcgatggga
      601 tacagaaggg gaggttcatg cgtccaagcc cggagggaca tgggtgtatg aggagcgcgc
      661 ccgtgtcatg aagcactgct gtacggcatc gcgggctcgg cgtacaccgc aagcgagaac
      721 actcatttcg tgagggggta agaatgggta gagagagcga cagggaatac cggagaaggg
      781 tgagcggcta ctaccgtcag ctgatggaga ggtgccaggc tcacatggac atgctcgagg
      841 ccgacctgcc gcctgcgcgc gagggtctgg gcgaggtcct cgaggaacga ggcgtcacca
      901 ggagggactt cctcaagtgg acctccgcca tgacggccgc cctgatgttg ccgcccatgt
      961 tcaggccgct ggtggcccgg gcagcggaga acttcggcag gcttcccgtg gtgtggctcc
     1021 acttcgcgga gtgcacgggg tgctccgagg cattcctcag gtcgtcctac cccaacgtgg
     1081 acgacatcct cctcgagacc atttccctcg agtatcacga gaccctcatg gccgccgccg
     1141 ggaaacaggc ggaggagtgt ctggaaaagg ccttggccga ctttccgggc aagttcgtgt
     1201 gcgtcataga gggtgcgctc cctacggggc tcgacgggca gtacctgcga ctcggcccca
     1261 agggcaagac cggcatcgag atagcgaagg aggtcacctc caaggcggcg gccaccatct
     1321 gcatcggcag ctgctcttcg ttcggcaacg tgcaggcggc gaaaccgaac ccgacggacg
     1381 ccaagggcat ctcggaggca atcggcatcc agaccgtgaa catctcggga tgcccgccga
     1441 gcccccagaa cttcgtgggc acggtgctgc acttcctcat gttcggcgcg ctgccgcccg
     1501 tcgacaacct gggaaggccc gtgtgggcat acgggaagcg catccacgac tactgcgagc
     1561 gcaggcctca ctacgatgcg ggcgaatacg tcgaggagtg gggcgacgag ggggcgaaga
     1621 aggggtggtg cctctacaag atggggtgca aggggccgta cacgtacagc aactgtgcca
     1681 aggtcaggtt caacgacggc gtatcctggc ccatcatggc cgggcacggg tgcatgggct
     1741 gcacggagcc cggcttctgg gacaccatgg ccccgctcga gaagccgctc acggacaagc
     1801 ccgtcggcgg gtggggcgtc acggcccgga tcgacaagct cggcctcggg ctgggcgcag
     1861 cgacgctcgt cggtatcggc gcccacgccc tggtgaccgg cgccctcaag cacggacagc
     1921 cggccaagcc cgagtccgac gagaagacgg accagggttc acacgaatag ccccatagcc
     1981 cacgggagga gagaacatgg cacagaggat agtcattgat ccgatcacca ggatcgaggg
     2041 tcatctgcgt atagaggtgg agctggacga taccaacacg gtcaaggacg catggagcag
     2101 catcacgttg tggcgcggca tcaagaccat cctcaagggc cgcgatccca gggatgcgcc
     2161 tctcatggtc cagcggttct gcggagtgtg cacctacgtg cactacgagg cgagcatcct
     2221 cgcgctggaa gacgccttcg gcgtgaggcc ccccgcaaac gcgaggctcg tgaggaacct
     2281 catccacgcg gccttgtaca tgaccgacca catcatgcac ttctaccacc tgcacgggct
     2341 cgactgggtc gacatcacca aggcgatctc ggccgacccg gcgaaggccg tcgaactggc
     2401 caagcagtac agcgacaatc cctacaactg ctcggagacc cattaccgcg ccgtgcagaa
     2461 aaggctcgcg aagtacgtgg agtccggcag gctcgggcct ttcgcgaacg cgtactgggg
     2521 caacccgtcg tacaagctat cccccgaggc gaacctcatc atcgtgtccc actacttgga
     2581 cgcgctccag gtctcgaaga caggcgcgca gatgatggcc atcttcggcg gaaagaaccc
     2641 ccacccgcag acgctggtcg cgggcggcat aacatccgtc atggacatgc tcgacgctca
     2701 ccggatcgcc gagtaccagt tcaggctcaa cgagctcaag cgcttcatcg acacggccta
     2761 cctgcccgac ctgctcctcg cggccggtgc ttacaaggac gagggcctgg cgggcgtggg
     2821 cgccggggtg aagaactaca tctgctacgg cgggttcccg ctcgacgacc agtggaacaa
     2881 gaccctgctg cccaagggcg tgatcgtcgg cggggacatc tccaagccgc tgccgctcga
     2941 cgaggcgaag atcaccgagg acgtgacgca ttcctggtac aagggcgaca agcccgctca
     3001 cccgtacgat ggttcgaccg agcccgagta tacggggctg gacgcgaacg gctacgtcaa
     3061 aggcaacgag aagtattcat ggtgcaaggg cccgtgctac gacggcaaac ccttcgaggt
     3121 ggggcctctc gcaaggatgg tgctcgggta cgcccagggc gacgagagga tcaagcccct
     3181 cgtggaagac accctcaagg ccacggggct gccggccacg gtgttgttct ccaccctcgg
     3241 gaggaccgcg gccaggggcc tggagacgaa gctcatagcg gactataccc agaactggat
     3301 aaacgagctc gtggcgaaca tcaaggccgg cgacacgagg acatggacca ggtgcgacgt
     3361 gccgaagtcg ggcaagggca gggggatggt agaggttccg agaggcgccc tgggtcactg
     3421 ggtcgtcata gaggaccacg tgatcgccaa ttaccaggcc gtcgtcccct cgacctggaa
     3481 ctgcgcgccg aggaaccagg acggcatgag gggcccgtac gaggaatccc ttatcgggac
     3541 gaagctcgcc aacccggaac agccgctcga gatcctgagg accatccact cgttcgaccc
     3601 gtgcatggcc tgtgccgtgc acatcatgcg gcccggctcg gacgagatca tgcacttccg
     3661 tgtggtctag cccggaggga gggggaacga gatggacacg agaactgcca aaaaggaatg
     3721 gagcctgtcc ttgagggcga accactggat catggccctg tgcatcttcg gcctcatcgc
     3781 gacaggtttc tacatcgcga gacccatcac gttcagcgcc ggagagacca tcgacaagtt
     3841 cgccatgggt tacgcacggt attttcacct gatgttcggg gtcacgctca ccttcctgtt
     3901 tgtctggagg gtgtacctga tgttcttctc ccgtttccac gccgactgga aggacttctt
     3961 cgcctggacc gacttcagga atttctggac gcagatcaag ttctacacct tcctgtccca
     4021 gaagaagccc gagcacacgc acctgtacgg cccgctgcag tccatcgcct acggggggtt
     4081 cttcgcgatg ctcctggtgc tgatcgtgac cgggctcatc ctggcagggg tggactacag
     4141 ctcagggttc ataggtacgg cgtacgcggt gttcaacccg atcacgaacg ccatcggcgg
     4201 gcttgcggtt gtaagggtgg tccatcacgt gttcacctgg ctcgtgatcc tgttcgtggt
     4261 cgtccacgtg tacatggcct tctggtacga catggtcttc aaggagggca cgatatcgtc
     4321 catgatcgga ggaaggatct tcaggaagca gcacgagtga cggcatcggg caaagggggg
     4381 ggagacaggc aaggacgcca tgtctcgagg gtctccccga agcggttcgg caccgaggca
     4441 gccgccggct tcgtgcccct tttcatgagg atccatcgaa aggggtcgtc ggatcataac
     4501 actacgatga gggggagaga cggagatctt tccccctctc ggtatgctgg gggacgaggc
     4561 tgaggtgata caaggggaaa agaagatcac ggtgctgggc ctgggcaaca tcctcctcaa
     4621 ggacgagggc ttcggcgtgc acttcgtgcg gtggttttcc gaaaggcatt cctatggccc
     4681 cgaggtggaa ctgctcgacg gcggcaccct cggctacagg ctgctcgata tcgtaacgag
     4741 caccaggcac ctgatc
//
LOCUS       DAOSCR010000139         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11340_length_4749_cov_3.170004, whole genome shotgun sequence.
ACCESSION   DAOSCR010000139 DAOSCR010000000
VERSION     DAOSCR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11340_length_4749_cov_3.170004"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLT69_04750"
     CDS             <1..951
                     /locus_tag="PLT69_04750"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HOJ13784.1"
                     /translation="RGHAVIVIECPNCRSKFRFVQPDPAKTTYMMRCSVCRHVFEHTI
                     DQGLSLEEEFDLLVQKAGTRQDKEQESPTLRPTEQEGIVETPGDVPEAVEEGAQVHVE
                     GEEPPTESVMREIDSILGAAEETAVTEEEAPVAVEETSRTRTVLKAAAAVACIVVIAA
                     ALGWFFKDRILPLSGRQDQGLPSQPKTAGPFFEIDERNVTHELLTHEQEGAVLVIRGT
                     LRKISGVPVESVMVEARVYDRAGALMENRIAYAGIVPDTSEFVRQPRADIDALLTSDP
                     ATPGSTLPGGDIPFAVAFFGKTAREASSYQVEVKDIRWRQ"
     gene            1031..1444
                     /gene="ruvX"
                     /locus_tag="PLT69_04755"
     CDS             1031..1444
                     /gene="ruvX"
                     /locus_tag="PLT69_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOJ13785.1"
                     /translation="MRILGIDYGTKRIGIAVSDPAGIMAHPLKVLEVRPDDSHLLEIQ
                     AIARSYGVEKIVVGLPYKMDGTEGPKAREACLWGERVAELTGLPVVFWDERLSSFEAE
                     ELLAGHEVSPKRMRKLVDKIAAGIILQGYLDSQKT"
     gene            1441..2451
                     /gene="mltG"
                     /locus_tag="PLT69_04760"
     CDS             1441..2451
                     /gene="mltG"
                     /locus_tag="PLT69_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOJ13786.1"
                     /translation="MSPKAHTVTTAIVVLAVGLAGHALSFVSTQVRPPETVLLEITRG
                     SGSWQISRTLEEKGVVSDAPTFMALALATGKARRLQAGIYVFEGAHRPLEVMDILFMG
                     KTLRHRVTIPEGYTIFQIAHAVAQTGVMERDAFLAKARDPQTASFFGIDAPSMEGFLF
                     PDTYFLVPRSTPLEVMAKMVGRFKQVYTQEMEERARTLGLSMREAVTLASLIAKEAKV
                     AHEKPLVSSVFHNRLRRGIRLQSDPTAIYGIDGFTGRIRHVDLLRESPYNTYLHKGLP
                     PGPICNPGEDSLKAALWPARSNYLYFFARGDGTHVFSATIEEHNRAISGITGPRGGRP
                     LF"
     assembly_gap    2640..2649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2650..3076
                     /locus_tag="PLT69_04765"
     CDS             <2650..3076
                     /locus_tag="PLT69_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13787.1"
                     /translation="GLLEAMSKAGVDVPFVLITGYASLDSAIEAVNKGAFSYIKKPFN
                     MEEIRIVVRKVRQRQDLVEKVRSLTLELEALRERMKTGGEDVGTPFLLAQPMQPVHSS
                     LDMNKALAAIEYLGRLKNDGLITESEFGEYKGKILKRIV"
     gene            3076..4503
                     /locus_tag="PLT69_04770"
     CDS             3076..4503
                     /locus_tag="PLT69_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13788.1"
                     /translation="MEDRVLIVDDDQGIRQTIRSIVEDLGYACDTAPDGQEALGLVES
                     RRYLCVFTDIMMPRMTGLELIKKIKARDISIPIIVITGYASLEIAIDAMKCGASDFLP
                     KPFKMSQIQILLNKMRREKTILEENRRFSDTLQLYRIIDNLKGQLEDKAREISSLKAI
                     SEKIVSLKGIRDLVGAIVDVSKQLIGNSEVKFYPVRGLPEPSRDNGPMPELQVPDELL
                     AGKIVRVDNSFLDGERHFQTLFPLTIEGQVFGALDVRTETLLDEEAETKILYLLNRSA
                     ERMENVALYEGLYENILSTLRSMAKILDARDPHTSQHSTRVTNFSIAIANALGLSAEE
                     RDVLYIASSLHDIGKVGIPDSILLKPDRLTEEEFTIIRKHPDIGADILKQLPPMYRET
                     EIIRHHHERYDGKGYPAGIGDNEIPLLSRIITIADAYDAMTSDRPYRKGLAVGMAVEE
                     ITRCAGSQFDPELARLFLHKVIGVF"
BASE COUNT          984 a         1554 c         1459 g          742 t
ORIGIN      
        1 aggggccatg ccgtgatcgt catcgagtgt ccaaactgcc gctccaagtt caggttcgtc
       61 cagccggacc ctgcaaagac gacgtacatg atgcgatgca gcgtatgcag gcacgtcttc
      121 gagcacacga tcgaccaagg cctttccctg gaagaagaat tcgacctcct cgtccagaag
      181 gcgggcacac ggcaggacaa ggagcaggag tcaccgaccc tgaggcccac ggaacaggaa
      241 ggcatcgtgg agacgcccgg ggatgtcccc gaagcggtcg aggaaggcgc ccaggttcac
      301 gtggagggtg aggaaccccc cacggaatcg gtcatgcgcg agatagactc gatcctgggt
      361 gcggcggaag agacggccgt aaccgaggag gaagcccccg tcgccgtcga ggagacctcc
      421 cgcacgagga ccgtcctgaa ggcggcggcc gccgtcgcct gcatcgtggt gatcgccgcg
      481 gccctcgggt ggttcttcaa ggacaggatc cttcccctct ccggcaggca ggaccaaggc
      541 ctgccctccc aacccaagac cgcggggccg ttcttcgaga tcgacgagag aaacgtcacc
      601 catgaactgc tcacgcacga gcaggaagga gcggtgctcg tgatcagggg caccctcaga
      661 aagatctcgg gagtgccggt ggaatcggtc atggtcgagg ccagggtgta cgaccgggca
      721 ggggccctga tggaaaatcg catcgcgtat gcaggcatcg tgcccgacac gagcgagttc
      781 gtgaggcagc cgcgggcaga catagacgcc ctcctcacct ccgaccccgc cacccccggc
      841 tccacgctgc cgggaggcga catacccttc gccgtcgcct tcttcggcaa gacggcgagg
      901 gaggcctcgt cgtaccaggt ggaggtcaag gatatccgtt ggagacagtg agacagcccc
      961 ccccccgcct caccgtccgg ccggaccggg agcatgcagt cgaaacacac ccccgaacca
     1021 gagtgatacc atgcgcatcc tcggcataga ttacgggacg aagaggatcg gcatcgcggt
     1081 cagcgacccc gccgggatca tggcccatcc actaaaggtc ctcgaggtga ggccggacga
     1141 ctcgcacctc ctcgagatac aagccatcgc tcgaagctac ggggtggaga agatcgtggt
     1201 cggcctcccg tacaaaatgg acgggaccga gggccccaag gcccgcgagg cctgcctttg
     1261 gggcgagcgt gtggccgagc tgaccggcct gccggtggtg ttctgggacg agcgtctctc
     1321 gtcgttcgag gccgaggagc tgctcgccgg tcacgaggtg agcccgaaaa ggatgaggaa
     1381 actcgtcgac aagatagccg ctgggatcat cctccaggga tacctggact cgcagaagac
     1441 atgagcccga aagcgcacac ggtgacgacg gcgatcgtgg ttctggccgt gggtttggcc
     1501 ggccacgccc tgtccttcgt ctcgacccag gtcaggccgc ccgagaccgt gctcctcgag
     1561 atcacgcgcg gctcgggttc gtggcagata tcgcgcaccc tcgaagagaa gggcgtcgtc
     1621 tcggacgcgc cgacgttcat ggcgctcgcg ctcgccacgg gaaaggcgag acggctccag
     1681 gcgggcatct acgtgttcga gggggctcac aggcccctcg aggtcatgga tatcctcttc
     1741 atgggcaaga ccctcaggca ccgtgtcacc atacccgagg gatacacgat cttccagatc
     1801 gcccatgccg tggcgcagac aggcgtcatg gagagggacg ccttcctggc gaaggccagg
     1861 gacccgcaga cggcctcgtt cttcggcata gacgccccat cgatggaggg cttcctcttc
     1921 ccggacacct acttcctcgt cccccgctcc acacccctcg aggtgatggc caagatggtc
     1981 ggccgcttca agcaggtcta cacgcaggag atggaagaac gtgcgcgcac tctcgggctc
     2041 tccatgagag aggccgtcac cctggcctcg ctcatcgcga aggaggcgaa ggtcgcccat
     2101 gagaagccgc tcgtctcgtc cgtcttccat aaccgcctac gccgaggcat acgcctccag
     2161 tcggacccta cggcgatcta cgggatcgac gggttcacgg gcaggatcag acacgtcgac
     2221 ctcctcaggg aatccccgta caacacctac ctgcacaagg gactgccgcc cgggcccata
     2281 tgcaaccccg gggaagattc cctgaaggcg gctttatggc ccgccagatc gaactacctc
     2341 tacttctttg caagggggga cggcacccac gtattctcgg ccaccatcga ggagcacaac
     2401 cgagcgatct cggggatcac aggccctcga ggcggccgac ctctcttcta aagtcgcgca
     2461 ccctcacgtt cgataaggga gatcatggcc gaagtacgcg catgcatcgt ggacgacgac
     2521 gactccatcc tcgacatcat agaacagatc ctggaggaag agggcgttac gagcaggagg
     2581 ttccacctgg ccgaggaggc cctcgcctcg atcgtttccg agcgctacga catggtcatn
     2641 nnnnnnnnnc gggctcctgg aggcgatgtc caaggcgggc gtcgacgtcc ccttcgtgct
     2701 catcacgggc tacgcatcac tggactccgc gattgaggcc gtgaacaagg gcgcgttctc
     2761 ctacatcaag aagccgttca acatggagga gatacgcatc gtcgtacgga aggtcaggca
     2821 gcgccaggac ctcgtcgaga aggtgaggag cctcacgctg gagctcgagg cgctcagaga
     2881 gcgcatgaag acgggcgggg aggacgtcgg cacccccttc ctgctcgccc agcccatgca
     2941 gcctgttcac tcatccttgg acatgaacaa ggccctcgcc gcgatcgaat acctggggcg
     3001 gctcaaaaac gacggcctca tcaccgagag cgagttcggc gagtacaagg ggaagatcct
     3061 caagaggata gtctgatgga agaccgcgtg ctcatagtgg acgacgacca ggggatacgc
     3121 cagactatcc gcagcatcgt ggaggacctc gggtacgcct gcgacacggc tccggacggc
     3181 caggaggcgc tcggcctcgt cgagtcgagg cgttacctgt gcgtgttcac ggatatcatg
     3241 atgcccagga tgacggggct cgagctcata aagaagatca aggcccggga catctccatt
     3301 cccatcatcg tgatcacggg ctacgcctcc ctggaaatcg cgatcgacgc catgaagtgc
     3361 ggcgcctcgg acttcctccc caagcccttc aagatgagcc agatccagat cctcctgaac
     3421 aagatgcgga gggagaagac gatcctggag gagaacagga ggttctccga caccctgcag
     3481 ctgtaccgga tcatagacaa cctcaagggc cagctcgagg acaaggcgag ggagataagc
     3541 tctctcaagg ccatatccga gaagatcgtg agcctcaagg gcatcaggga cctggtgggc
     3601 gccatcgtgg acgtctccaa gcagctgatc ggaaactcgg aggtgaagtt ctaccccgtg
     3661 agaggactgc ccgagccttc ccgcgacaac ggccccatgc cggagctgca ggtgcccgac
     3721 gagctcctcg ccgggaagat cgtcagggtc gacaactcgt ttctcgacgg cgagcgccat
     3781 ttccagacgc tcttccccct cacgatcgag ggccaggtct tcggggccct cgacgtcagg
     3841 accgagaccc tcctcgacga agaggccgag accaagatcc tctacctgct gaaccgctcc
     3901 gccgagcgca tggagaacgt ggccctctac gaggggctct acgagaacat cctctccacc
     3961 ttgaggagca tggccaagat cctggatgca cgggaccctc acacgtccca gcattccacc
     4021 agggtcacca acttctccat cgcgatcgcc aacgccctcg ggctgtcggc agaagagcgg
     4081 gacgtgctct acatcgcctc gtcgttgcac gacatcggca aggtgggcat cccggacagc
     4141 atcctcctca agccggacag gctcaccgag gaggagttca ccatcatcag gaaacacccc
     4201 gatatcgggg cggacatcct caagcagctt ccgcccatgt accgcgagac ggagatcata
     4261 aggcatcacc acgaacggta cgacgggaag ggataccccg ccggcatcgg ggacaacgag
     4321 atccccctcc tctcgcgcat cataaccatc gccgacgcgt acgacgccat gacctcggac
     4381 aggccttacc gcaaggggct ggccgtcggg atggcggtcg aggagatcac caggtgcgcg
     4441 gggtcgcagt tcgaccccga gctcgccagg ctcttcctcc acaaggtgat cggcgtgttc
     4501 taaggtaggg gtcccccgcg tcgcaccttg ccgccttctt gcccgaggac aggccccaag
     4561 catcgacacc ccgtctccga cccggggaaa tcaggtacat ctcccgccgg gatcccggcc
     4621 tggagtcggc cggcctcttc cccggagcac catgcgttcc gcacggctgg acgcagggcg
     4681 ctccttcctg agcctcaccg acttcgagcc gtcacgcacc tcgagctctc ccaggcaggc
     4741 gccgacagc
//
LOCUS       DAOSCR010000140         4745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11356_length_4745_cov_3.052239, whole genome shotgun sequence.
ACCESSION   DAOSCR010000140 DAOSCR010000000
VERSION     DAOSCR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11356_length_4745_cov_3.052239"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..143
                     /locus_tag="PLT69_04775"
                     /pseudo
     CDS             1..143
                     /locus_tag="PLT69_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            699..1226
                     /locus_tag="PLT69_04780"
     CDS             699..1226
                     /locus_tag="PLT69_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ13789.1"
                     /translation="MVRLGLVDSFFGNISIRTGGSISISQTGASLDELQGCIDRACMD
                     GSSSCEITSSSELFSHVRIYELTGARVILHGHPRCAVILSLAEGPIPFGETRYVLGVP
                     VVSGEVGAGSRGIVHTLPAAMEGSLEAIVAGHGVFAASRSSVREAFERLLSVENRCYE
                     ECARRIKAIDERRQP"
     gene            1592..1939
                     /locus_tag="PLT69_04785"
     CDS             1592..1939
                     /locus_tag="PLT69_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13790.1"
                     /translation="MSKILSACLAVLVVCTWARGASAIEFAIIKNAMFCGQKIEVYKS
                     LDEALENYTGEGKIFEITLKPVPMKRVENKKKIEVSEFTWKVDEQYLKDLRSRQGKNT
                     KGSKSAAQEKKTP"
     gene            2179..2460
                     /locus_tag="PLT69_04790"
     CDS             2179..2460
                     /locus_tag="PLT69_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13791.1"
                     /translation="MDKEETELQAKKILIQKKHELHEETLARVIHDLEDTLERYEKYS
                     GPESIFAFVRANAAALLFLCQERKRDRDKRKGDYTKPAQELPPSGSDLL"
     gene            2908..4164
                     /locus_tag="PLT69_04795"
     CDS             2908..4164
                     /locus_tag="PLT69_04795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ13792.1"
                     /translation="MAHAKTPLFLRFRISAPTTVVIFVVLLASLVSAAGQDPATETQG
                     GNRAQAVREGSSDRTIPLKKAVVIRKGDIFSEILVRQGVSLADALEVSRKANKVCRLT
                     SLKPGDVLELSFTPDGVGLEEIAHKAPGKKPLVLYHSSVVAIAGTRQKAPVATVATDP
                     GRAIPQKRKDVSRADASPETSDLDASHDMSLVSSYDLGPLPVLRHPAQLKYELLAQGK
                     VLSPIAPLWVTSVHPTPFDHSKDKGALSIAKAKERLGSKQRHPSKSASRKKTDDGFLR
                     APVSYRYVSSGFTASRVHPITGDVLPHYGVDFAAHMGTPVHAVGSGRVIFAGWDGGFG
                     KVVRIRHTNGYITHYGHLSKFAKGIRPGKKVKRGQTIGYVGMTGVATGPHLDFRITRN
                     GRFVNPFTILNNSKRVTAKKSRHHRG"
BASE COUNT          959 a         1529 c         1461 g          796 t
ORIGIN      
        1 agaagctcgt cttcaaccgc gttgccaagt attactacta cccgggctgg tgggagggtg
       61 gcccccagct gtccctctac gtcaatgcaa agaagttcgc cgagctgccc aaggaatacc
      121 agaccatcct cgaggatgcc tgcctctacg ccctcgacta tgagctgttt tccaacaggc
      181 cccaggtgcc gaaggcagtg cgggccctct tcgacaggct gaacatcaac ggtctcatcg
      241 tggccaagcc ggaagagacc aggtggtcga tcatgcgacg cctcgccgag accgggggca
      301 acccggtgac accctcggac tcggagagcg tcaccttcct tcacgacatc cccgtggtcg
      361 ggtctctgca ttccgaggat ctcttcgagg tactggcccg ccgcaagggg tgcatcgtcg
      421 aggacgcggg cgtcgtcgcg actggcaccg tctgcctgga gcaggccttc gtgaccttca
      481 gttccatctg ctttgcgacc ttcgtcaagt acttcgccga cctgatcggg aaacccggcg
      541 actcgtgcgc cctcaccggt cgcacccggg aagacagggc gaaggccctc aaggcactcg
      601 agaccgcacg gccgaagggc cggccggcag gcctggccga cgagataccg tccgacgagg
      661 ccgggatcgt ctcggccatg gacagggcag gcaaggagat ggtccgcctg gggctggtcg
      721 actccttctt cggcaacatc tcgatcagaa ccggcggttc gatctctatt tcccagaccg
      781 gggcgtcgct ggacgagctg caggggtgca tcgacagggc ctgcatggac gggagctcga
      841 gctgcgagat cacctcgtcg tcggaactct tctcccacgt ccgcatatac gagctcaccg
      901 gggcccgcgt gatcctgcac ggtcaccccc gatgcgccgt gatcctctcc ctggccgagg
      961 ggcccatacc attcggcgag acgaggtacg tcttgggggt tccggtggtt tccggggagg
     1021 tgggcgccgg ttcgcgcggg atcgtgcaca ccctccctgc agccatggag ggatccctcg
     1081 aggcgatcgt cgccgggcac ggcgtgttcg cggcctcgcg atcctccgtg cgggaggcgt
     1141 tcgagaggct cctttctgtg gaaaaccgct gttacgagga atgcgcacgg aggatcaaag
     1201 cgatcgacga acgccggcag ccgtgacgag ggaacccggc gagcgccctc gtcttcggtt
     1261 cctgtccacg gcgatgtcga tcgcccctcc gtcccggcaa cgacgagcga cgcccgcacg
     1321 cgatcggggc gcccatgcac agcgattcca aggttcgtct tcgcagacgt gcaggacgtc
     1381 gtcgccccgg tcgaagacct cggggaaacg cggcggaccc ttgacgtacc ccgatggttg
     1441 accgtgatga tatgcccgcc gatcatcacc ctcggggaaa cccgccgggc cctcgacgta
     1501 ccccgcctcg gggttgaggc ggcggcgcgc acgcatctcc gtgttgacat ctcgggtgaa
     1561 atgccccaca cactagccag gagggtggcc tatgagcaag atcctctctg catgccttgc
     1621 ggtcctcgtg gtctgcacgt gggcgagggg cgcctcggcc attgaattcg ccatcatcaa
     1681 gaacgccatg ttctgcggcc agaagataga ggtctacaag agcctggatg aggcgctgga
     1741 gaactacacc ggcgagggca agatcttcga gatcacgctc aagcctgtgc cgatgaagag
     1801 ggtcgagaac aagaagaaga tcgaggtctc ggaattcacc tggaaggtgg acgagcagta
     1861 tctcaaggat ctcaggagca ggcaggggaa gaacacgaaa gggtccaagt ccgccgccca
     1921 ggaaaagaag accccctgag agacacctgc cccggacgtg gacgaaaggg ctgtcccgac
     1981 tcttcaatac ggataaacct tggtccctcc catgactgaa acacggtgct cacatggaca
     2041 tacgggcagg ggacattgag aaagagttta gcaagggaaa tttctcatga gccttgacaa
     2101 cgtggacgaa aaggattgac cggcgaggat cgttggccta acctatcgcg ggagaatgca
     2161 tgcaggggga ttgagaccat ggacaaggaa gaaaccgaac tccaggcgaa gaagatcctg
     2221 atacagaaga agcacgaact ccacgaggag accctcgcaa gggtcattca cgacctcgag
     2281 gacacgttgg agaggtacga gaagtactct gggcccgaga gcatcttcgc tttcgtgagg
     2341 gccaacgcgg cggcgctgct cttcctctgt caggagagaa aacgcgaccg agacaagcgc
     2401 aagggggatt acacgaaacc cgcccaggaa ctcccacctt ccgggagcga cctcctgtag
     2461 ccatccccag gcaaaccacc gccttgccgg gtcgtatccc gcagcttgcc tgcaggggga
     2521 acgcaccggt gcccaggggg catacccggg ccatcccgtt gagccgatcc ttgcaggctc
     2581 gccaaggggt aggcgctcac tgaacgagcg acaggcacac cctcgacccc gacattcttt
     2641 gccatgcgcc catccgtgca cgggcgtgac aggaaagagc cctgtccggc cgaaggccgg
     2701 cacattcggg ccgacgccag agacggggct tcctgcgtgc atgcattcca cccggaccca
     2761 actatcgtga agttcaacgc atttttttga ttttactgga gtatatctga acttgccatt
     2821 tctttgatcc tggtgtatag atcccctcga tgcattgcat gcctgatacc gttcacttca
     2881 gggcattcca aggggaggtt ccatcccatg gcacatgcaa aaaccccgct ctttctgcgg
     2941 tttcgtatat cggcaccgac gacagtcgtg atcttcgtgg tccttcttgc ctcgctcgtg
     3001 agcgcagccg gtcaggaccc ggccaccgaa actcaggggg gaaaccgggc acaggccgtc
     3061 cgagaggggt cctctgaccg gacgatcccc ctcaagaagg cggtggtcat tcgcaagggg
     3121 gacatcttct cggagatact cgtgcggcaa ggcgtcagcc tggccgacgc cctcgaggtc
     3181 tccaggaagg cgaacaaggt ctgcaggctc acgagcctga aacccgggga tgtgctcgag
     3241 ctttccttca cccccgacgg ggtcgggctc gaggagatcg cccacaaggc gccgggcaag
     3301 aaacccctgg tcctgtacca ctccagcgtc gtcgccatcg ccggaacccg ccagaaagca
     3361 ccagtggcga ccgtggccac cgaccccggc cgggcgattc ctcaaaagcg gaaggacgta
     3421 tcgagggcgg atgcctcccc ggaaacgtcc gacctcgacg catcacatga catgagcctc
     3481 gtgtcctcct atgacctcgg ccccctcccc gttctgcgac accctgccca actcaaatac
     3541 gagctgctcg ctcagggaaa ggtgctctcc cccatagccc cgctgtgggt cacctcggtg
     3601 cacccgacac ctttcgacca ctcgaaagac aagggcgccc tctccatcgc caaggcgaaa
     3661 gagcgtttgg gctccaagca gcgccacccg tcgaaatccg cctcacggaa aaagaccgac
     3721 gacggcttcc tgagggcacc ggtgtcctac aggtacgtat cctcaggatt cacggcgagc
     3781 agggtgcacc cgatcaccgg ggacgtgctt ccccattacg gggtcgactt cgccgcgcac
     3841 atgggcaccc ccgtacacgc cgtgggctca ggcagggtga tcttcgccgg gtgggacggc
     3901 ggtttcggga aggtggtccg cattcgccac accaacgggt acataaccca ctacggccat
     3961 ctctcgaagt tcgccaaggg aatcaggccc ggcaagaagg tcaagcgcgg tcagacgata
     4021 gggtacgtgg gcatgacggg agtggccaca ggccctcacc tggacttcag gatcacgcgc
     4081 aacggccggt tcgtcaaccc ctttaccatc ctgaacaact caaagagggt cacggccaag
     4141 aagtcgcgac accacagggg ttgagcgccg caacgagata cccgtccctc ctctccttgt
     4201 ccctggaggt cggtcgtcgc ccgcaggaca gagggcgccc ctggacgctc ccttcccacg
     4261 ccagcggcag gcaaccagca aaaaccgcct cggcaatgga ggggatcgcg agggtcacag
     4321 ggggctggaa gatcaccggc gcggcaactc gccacggtct tcggccacga gggggttgtc
     4381 gtgcaggatg ggaggtccgt gctggagggg ctctttcccg aaccattcct gtgcttgccc
     4441 gtacgcgccg gcctgcttca aggggctccc ccgcacgcca ccagggggga gaacgacgcg
     4501 ggtgggcggc ccgtccgccc gcggcaagcc ccctccccat gggacgccga gccgggggtc
     4561 catcgtctgc gcttaagcac gcatgctcag gcgtagacga ccctcgttca catccaaccc
     4621 cgccgaacat gcggccttcc ccgcttgacc ggggaatgcc cgctgtatat ggtacacgtg
     4681 tatgagagcg ctgagaaagg gccgccattg tcgacaagga cggacgtgat catcatcggt
     4741 ggtgg
//
LOCUS       DAOSCR010000141         4720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11436_length_4720_cov_3.414362, whole genome shotgun sequence.
ACCESSION   DAOSCR010000141 DAOSCR010000000
VERSION     DAOSCR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11436_length_4720_cov_3.414362"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PLT69_04800"
     CDS             complement(<1..1906)
                     /locus_tag="PLT69_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HOJ13793.1"
                     /translation="MKLSFKMNLFLASVVVVLIVGLSAVFRMVVLESFYELDRKHAED
                     AALRCRESLMREIVHLGSFVHDWSSWDDTYRYVVDRNEEFIQSNITKEVFEDQEINLI
                     HIYDASRRALSAKSYGMDLEREIDLDLAEELGPEIFAMLVDQKDPSRGTDGLVMTSYG
                     PLLVSARPILTSENKGPARGTMVMGRFLSGGIMEAISQQSGVPAGFVPAVGEAGAAGD
                     TTTRSGAANLDDVRVVVDPSGDFSTASVVIKDIAGRPAIVLSARVDRAIAGKGLRAYF
                     LGLVSLMVLGVAAMVSVYLLTRSMVIEPIEKLAQKANSTLDGKNENVASLSGRDDEIG
                     ELARRFDALFGDLARQNKMLEVQEKRLAQIIDFLPDATYAVDREGVVIAWNRAMEEMT
                     GMPKGEVMGRDHGHVARRFFGTQAGMLVDLVLGKPLEGDGRWFNLVREDSARVTAECH
                     APSLSEGRGAYLVSAAAPLFDEGGVIAGAIECLRDVTEKKRAEEVLERLGRTVEQLHD
                     GVLMTGLDGTVIYANPAWASMHRLEAEEVCGRHVSVFHTEVQMEQTVKPFMAEVTSRG
                     SGRAVISRITSHDEIVETLTSAFLLKDGRKNPTAIVWIARDITQETRMEEQLRQAQKM
                     EVVGRLAGGVA"
     gene            complement(2310..3299)
                     /locus_tag="PLT69_04805"
     CDS             complement(2310..3299)
                     /locus_tag="PLT69_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13794.1"
                     /translation="MREEIVRVRDLVKRFPVQRGILSGFLSSEARAVHAVDGVSFVIS
                     KGEVLGLVGESGSGKTTTGRLILGLTRPSAGEISYRGRPLASFGRAEMERLRTRMQVI
                     FQDPYASLSPRMTVGDAVSHPLKIHGMASGERAREMAEEIMEKVGLTPAGFLYRKYPH
                     QLSGGQRQRVAIARALVTSPDFVVADEPIAMADVSVRAMILELMVRLKEDFDLTYLFI
                     THDLATCKYICDRIAVMYLGRIVEIGPIREVFSNPVHPYTRTLLEAVPVPDPEFRRTQ
                     PMPKGEIPNPVDPPAGCAFHPRCAYAEPACARERPELLPVGPGHEAACPVMAR"
     gene            complement(3296..4252)
                     /locus_tag="PLT69_04810"
     CDS             complement(3296..4252)
                     /locus_tag="PLT69_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13795.1"
                     /translation="MLSIKGLHVEFPISIGTVKALQGVDLDIVKGEVMGLVGESGCGK
                     STLGFSIVRLLRPPGRITSGRIDYRGRNVAGMDEKELLRIRGRRIAMVFQDPLTSLNP
                     LFSIGQHFVETIRTHDRAVTKSRALEMAGDMLESLGISRQRLGEYPHQMSGGMRQRIM
                     IGLALVMRPELLIADEPTTALDVIVEAQFLDLLEELRGRFDLTVILITHNLGNVAQLA
                     HRITVMYGGTIVEVAPAGDLFRRPLHPYTEGLLASIPNIRLDRPRLVTMPGSPPDLVD
                     PPAGCVFHPRCPRAMDVCRRERPPSVSGDGRTVSCWLYKEVP"
     gene            complement(4273..>4720)
                     /locus_tag="PLT69_04815"
     CDS             complement(4273..>4720)
                     /locus_tag="PLT69_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ13796.1"
                     /translation="AMFGPGVINITFSVAIIYIPTYFRLVRGQTLAVKQELYVEAARA
                     IGAGHLTILAKYIFPNVIATTVVVFTLNVADAIMIEAALTYLGLGLPPTVVDWGMDLA
                     MGKKFLPSGMWWLVTFPGVMISLLALGFTMLGEGLAEILNPRLLER"
BASE COUNT          798 a         1572 c         1481 g          869 t
ORIGIN      
        1 aggccacccc ccctgcgagc cttcccacca cctccatctt ctgggcctgc ctcagctgtt
       61 cctccatcct ggtctcctgg gtgatgtccc gcgctatcca gacaatggcg gtggggttct
      121 tcctgccgtc cttgagcaga aaggcgctgg tcagggtctc aacgatctcg tcgtgtgagg
      181 tgatccggga gatcaccgcc ctgcccgacc cccgggacgt cacctcggcc atgaagggct
      241 tgaccgtttg ctccatctgg acctcggtgt ggaacacgct cacatgcctg ccgcatacct
      301 cttcggcctc gagcctgtgc atcgaggccc atgccgggtt tgcatagatg acagtcccgt
      361 cgagcccggt catgagcacg ccgtcgtgaa gctgttcgac ggtgcggccg agcctttcga
      421 gtacttcctc ggccctcttc ttctccgtga cgtcccgcag gcactcgatg gcgccggcga
      481 tgacgcctcc ctcgtcgaac aggggggccg cggcgctcac caggtatgcg cccctgcctt
      541 cggacaggct tggggcgtgg cactcggccg tcacccgtgc ggagtcttcg cgcaccaggt
      601 tgaaccagcg cccatcaccc tcaaggggct ttcccaggac gaggtccacg agcatgccgg
      661 cctgcgtacc gaagaaccgt cgggcgacat gcccgtggtc ccggcccatg acctctccct
      721 tgggcatgcc ggtcatctcc tccatggccc tgttccaggc gatcacgaca ccttccctgt
      781 ccaccgcgta cgtcgcgtcg ggcaggaaat cgatgatctg ggcgagcctc ttttcctgga
      841 cctccagcat cttgttctgc cttgcgaggt cgccgaacag ggcgtcgaac cgcctcgcga
      901 gctccccgat ctcgtcgtct cgacccgaaa gggacgccac gttctcgttc tttccgtcga
      961 gagtgctgtt ggccttctgg gcgagcttct cgatgggctc tatcaccatt gagcgcgtga
     1021 ggaggtatac cgagaccatc gccgcgacac cgagaaccat gagcgacacg agccccagga
     1081 agtaggcccg aagacccttt cccgcaatgg cgcggtccac cctcgcgctc aagacgatcg
     1141 cgggcctgcc cgcgatatcc ttgatcacga cgctcgcagt cgagaaatca cctgatgggt
     1201 caacgaccac cctgacgtcg tcaaggttcg cagccccgga acgggtagtc gtgtccccgg
     1261 ccgcaccggc ctcacccacg gcgggtacga accccgccgg gacgcctgac tgctgggata
     1321 tcgcctccat gatcccgccg gagagaaacc gtcccatgac catggtaccc cttgccggtc
     1381 ctttgttctc ggaggtgagg atgggtctgg ctgatacgag gagggggccg tagctcgtca
     1441 tcaccaggcc gtcggtgccc cgggaagggt ccttctggtc cacgagcatg gcgaatatct
     1501 cggggccgag ctcctcggcg aggtcaaggt cgatctccct ttccagatcc atgccgtagc
     1561 tcttcgcgct gagcgccctc ctcgacgcgt cgtagatgtg tatgaggttg atctcctggt
     1621 cctcgaagac ctctttcgtg atgttcgact gaatgaactc ctcgttcctg tcgacgacat
     1681 agcggtaggt gtcgtcccag gacgaccagt cgtgaacgaa cgaacccagg tgcacgatct
     1741 ccctcatgag gctctccctg cacctcagtg ccgcatcctc ggcatgtttg cggtcgagct
     1801 cgtagaagct ctcgagcacc accatcctga acaccgcaga caagccgacg atgagcacga
     1861 cgaccacgga agcgaggaag aggttcatct tgaaggagag cttcatcttg tatcgggatc
     1921 ctcctcaggc atgagagggc accgtcagta cggcctcctg atttatatcg gcagagtgac
     1981 aggttcctca aggggtttct tcccccatgt cgccggtggg atacctgtct ggtatccacg
     2041 tcgatgcgca tgcgccttcc cagaacccca tgggtgaagg acatgccagg aatcccccat
     2101 cccttgcatg cggccccctc gggcatggcc gcccgacaac ccgcaggggt tcgcccgtga
     2161 gactcccgtg cgtcgcacag accgcctggt tatggatgtt cacggtctcc ttccagggta
     2221 tgcccccatg tgcgtaggct gtcccgccgc ggcgatgacc gcgggcgcgt gtgacgatcg
     2281 agggctacgg gagcgaggat gccggagcgc tacctggcca tgacaggaca agcggcctcg
     2341 tgacccggcc cgacaggcaa gagttcgggc ctctcgcggg cacatgcggg ctcggcgtac
     2401 gcgcagcgcg ggtggaacgc gcatccggcc ggtgggtcga cggggttcgg gatctcgccc
     2461 ttgggcatgg gctgggtcct cctgaactcg gggtccggca cggggacggc ctccagcagc
     2521 gtcctcgtgt aggggtgcac ggggttcgag aagacctccc gtatgggccc tatctccacg
     2581 atccggccca ggtacatgac ggctatgcgg tcgcagatgt acttgcaggt cgccaggtcg
     2641 tgcgtgatga acaggtaggt gaggtcgaaa tcctccttga gcctgaccat gagctcgagg
     2701 atcatggcgc gcacggagac gtccgccatg gcgatcggct cgtcggcgac cacgaagtcc
     2761 ggcgaggtca ccagggcgcg cgcgatggcc acccgctgtc gttgcccgcc agacagctgg
     2821 tgagggtact tccggtagag gaaaccggcg ggcgtcaggc cgaccttctc catgatctcc
     2881 tcggccatct cgcgggcacg ctcaccgctt gccatcccgt ggatcttcag ggggtgcgac
     2941 acggcgtcgc ccaccgtcat gcgggggctc aacgaggcgt aggggtcctg gaagatcacc
     3001 tgcatcctcg tgcgcagccg ttccatctcg gcgcggccga acgaggccag ggggcggccc
     3061 cggtacgaga tctcgcctgc gctcggccgt gtgagcccga ggatgagcct ccccgtggtg
     3121 gtcttgcccg acccgctctc tcccacgagt cccagcacct ctcccttcga gatgacgaag
     3181 gacacgccgt ccactgcgtg cacagccctg gcctcactgg acaggaagcc ggagaggatc
     3241 ccgcgctgaa ccgggaagcg cttgacgagg tccctgaccc tgacgatctc ctccctcacg
     3301 gcacctcctt gtacagccag cagctcacgg tcctgccgtc gccgcttacc gacgggggcc
     3361 tctcgcggcg gcatacgtcc atggcacggg ggcacctcgg gtgaaacacg cagcccgcgg
     3421 gcgggtcgac gaggtcggga gggctgcccg gcattgtgac gagcctcggc ctgtcgagcc
     3481 tgatgttggg gatcgatgcg agcaacccct cggtgtaggg gtgcagaggc ctcctgaaaa
     3541 ggtcccctgc aggggccacc tccacgatcg tcccgccgta catcacggtg atcctgtggg
     3601 cgagctgggc cacgtttccc aggttgtgcg tgatgaggat cacggtcagg tcgaagcgcc
     3661 cgcgcagttc ctccaggaga tccaggaact gggcctcgac gatcacgtcc agggcggtgg
     3721 tgggctcgtc ggcgatcagg agttcaggcc tcatgacgag ggcgagcccg atcatgatgc
     3781 gctgcctcat gccccccgac atctggtggg ggtactcgcc gagccgctgc ctggagatcc
     3841 cgaggctctc gagcatgtcg ccggccatct cgagggcccg ggacttcgtg acggccctgt
     3901 catgggtccg tatggtctcc acgaagtgct gccctatcga aaagagcggg ttgagggagg
     3961 tcagggggtc ctggaagacc atggctatac gcctgcccct gatacggaga agttccttct
     4021 cgtccatgcc agccacattt ctgccccggt agtcgatcct gccggatgtg atcctccccg
     4081 gcggcctcaa aaggcgcacg atggagaaac cgagcgtcga cttgccgcac cccgactcgc
     4141 cgacaagccc catgacctcg cccttcacga tgtcgaggtc caccccctgg agcgctttca
     4201 ccgtcccgat ggagatggga aactcgacgt gcaacccctt aatggagagg atggtctccc
     4261 ggcgcccatg tctcaacgct ccagcagtct cgggttgagg atctcggcca gcccctcccc
     4321 gagcatggtg aacccgaggg ccaagagaga gatcatcact ccggggaacg tgacgagcca
     4381 ccacatgcct gagggaagga acttcttgcc catggcgagg tccatccccc agtcgacgac
     4441 cgtcggcggc aggccgaggc cgaggtacgt gagtgcggcc tcgatcatga tggcgtccgc
     4501 cacgttcagc gtgaagacca ccaccgtggt ggctatcacg ttggggaaga tgtacttcgc
     4561 caggatcgtg aggtgtcccg cccctatggc gcgcgcggcc tccacgtaga gctcctgctt
     4621 gacggccagg gtctggcccc tcacgaggcg gaagtacgtg gggatgtaga tgatggccac
     4681 ggagaaggtg atgttgatca cgcccgggcc gaacatggcc
//
LOCUS       DAOSCR010000142         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11439_length_4719_cov_3.302530, whole genome shotgun sequence.
ACCESSION   DAOSCR010000142 DAOSCR010000000
VERSION     DAOSCR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_4719_cov_3.302530"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLT69_04820"
     CDS             <1..327
                     /locus_tag="PLT69_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HOJ13797.1"
                     /translation="TGIGTAEIERYGLRHVGSTVIKARTLPGYFPGSSDIVVKLFFEN
                     ETGVLVGGQIAGGPGSALRINVIAAAVTMRMRVDELYTLDTAYAPPISPVWDPLLIAA
                     RSAMKT"
     gene            complement(361..780)
                     /locus_tag="PLT69_04825"
     CDS             complement(361..780)
                     /locus_tag="PLT69_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13798.1"
                     /translation="MSEATVDNRAQDVPFSFVIADLLSSNLEQRPEKMRTFSKMWGVV
                     GLNLPDIDAAITMIFAGGRVRIEPGIVGKPDIVITSSSEKIISLNSISIKFGLPYYFD
                     EAGLAVLRQLATGELKIQGMFTHPVLLTRLTKIMSVM"
     gene            complement(839..2002)
                     /locus_tag="PLT69_04830"
     CDS             complement(839..2002)
                     /locus_tag="PLT69_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ13799.1"
                     /translation="MTYDTDLSFNYYGPTKVIYGVDSSKDVEIEMGSIGGTRAVVVTD
                     QGIIKAGLTDHIIKALGNKCVGVFSDVPQDTGVEVVDACADFARKGGADTVISIGGGS
                     VIDTAKGVCILLTEGGSLRDYSGVQLLSRPQTPHIAIPTTAGTGSEVTNAAVIMDKEM
                     GQKRLMVENYNIPRIGILDPKLTAKLPPLLTAGTGMDAMTHAVEAIHAIPHEPITDGL
                     AMHAIRLLSRYLPVCVAKGDDLAARGQVQIAATMAGWAFSIAGVGNVHALAHSIGAVA
                     HVPHGIANGILLAACMEFNLETCPESYALVAEAFGVREKGMDDMEAARAAIDAVKEFT
                     KKIGHPQRLSEMNVTEDDIVKAADLSLGDGAIVNNPRVVLDASEVLEIYRKVL"
     gene            complement(2082..2507)
                     /locus_tag="PLT69_04835"
     CDS             complement(2082..2507)
                     /locus_tag="PLT69_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOJ13800.1"
                     /translation="MGKIKAMTQEWMDAYKAAIEADPEYKEIAKNWEGSVALIVNPDP
                     DKGVPEPVYLFMDYWHGDVGEFVVCDKAKAESAKFVMTGDYARWKQVALKELDSTKAL
                     LQGKLKMKGDLPYVVRNIKVVNKVIDILGRVDTLWPDEA"
     gene            complement(2540..4399)
                     /locus_tag="PLT69_04840"
     CDS             complement(2540..4399)
                     /locus_tag="PLT69_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOJ13801.1"
                     /translation="MPNGYAGAILLVDLTNRTTDELKVDETLLKDFIGGSSLAARLLL
                     DRFKLDADPLSGDNPLMIMTGPMVASGFPGTSRFSICARAPQTGIWGESACGGTFGPE
                     LKRAGYDGIIVTGVSSSPVYLSVVDGKAGIHDAKHLWGKDIYETTDELKAKDGGLKVL
                     AIGQAGENLVKIAAIGNDKGHFSGRTGLGAVMGSKRLKAIVARGKGRPAKADEERFKE
                     VMKKALAEVKDSALAEGLHLMGSDANMELGMIKGDVPIKNWTVGEAFDLASNLSGPTM
                     REKYLTKEHACANCPVACKRVVKVADGPYRTEEGPGPEYETCATFGTMLMNDDLAAVI
                     KANELCNRYGMDTISCGSAIALAMELYEKGIVTPSDTGGLELTWGNMDAVMPLIKNIA
                     FREGFGDILAEGTLGAARRIGRGAEDMVAHVKGLEAPMHDPRGFHGMGLAYMMSNRGA
                     CHLQHAVLATEQGMASWPELFDMEDDYVGTTSEGKAMLVFNSENYGILCNSLSICHYV
                     VYILRPETVLEAFNAVTGFGLGKDDLLKAGMRDWTLKRGINNLLGISRKDDVLPKRIL
                     TALSEGAAAGSVPDMDLLLKEYYALRGLDERGFPKPEKLDELGLKELKRRLYQ"
     gene            complement(4573..>4719)
                     /locus_tag="PLT69_04845"
     CDS             complement(4573..>4719)
                     /locus_tag="PLT69_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ13802.1"
                     /translation="QGKSTSEIASAMNLSVSTIGTYRERIKEKLNLKNAAELVRYAVH
                     WVDG"
BASE COUNT          719 a         1554 c         1498 g          948 t
ORIGIN      
        1 acggggatag gcaccgcgga gatcgagcgg tacggtctca ggcacgtcgg gtccacggtg
       61 ataaaggcga ggaccctgcc gggttacttc ccgggctcgt cggacatcgt ggtgaagctc
      121 ttcttcgaga acgagaccgg ggtgctcgtg gggggacaga tcgcgggcgg gcccggctcc
      181 gcgctcagga tcaacgtgat cgcggccgcc gtgaccatgc gcatgcgggt ggacgagctc
      241 tataccctcg acacggcgta cgcgccgccc atatcccccg tgtgggaccc gctgctcatc
      301 gcggccagga gcgccatgaa gacgtgacgc ggggccgagg cggccgagcc gccgggcccc
      361 tcacatgacc gacatgatct tggtcagcct tgtgagcagc acggggtggg tgaacatgcc
      421 ctggatcttg agttcccccg tggccagctg cctgagcacg gcgagcccag cctcgtcgaa
      481 gtagtagggg agcccgaact tgatggatat ggagttcagg gagatgatct tctctgagct
      541 cgaggtgatc acgatgtcgg gcttgcccac gatccccggc tcgatcctca cgcggccccc
      601 ggcgaagatc atggtgatgg ccgcgtcgat gtcgggaagg ttgagcccca ccacccccca
      661 catcttcgag aaggtgcgca tcttctccgg cctctgctcg aggtttgagg agagcaggtc
      721 ggcgatcacg aaagagaagg gcacatcctg cgccctgttg tcgactgtcg cttctgacat
      781 gaacgagtcc tcccgtacaa gtggtacgcc cgcccgtcgc cgggcgggcg caagccttct
      841 agagcacctt tctgtagatc tccagcacct cggaggcgtc cagcaccacg cggggattgt
      901 tcacgatggc gccgtcgccg aggctcaaat cggcggcctt gacgatgtcg tcctcggtca
      961 cgttcatctc ggagagtctc tgcgggtggc ctatcttctt ggtgaactcc ttcacggcgt
     1021 ctatcgctgc ccttgccgct tccatgtcgt ccatgccctt ttccctcacg ccaaaggcct
     1081 cggcgacgag ggcgtagctc tcggggcagg tctcgaggtt gaactccatg caggccgcca
     1141 ggaggatgcc gttggcgatt ccgtgcggca cgtgcgccac tgcgcctatg gagtgggcca
     1201 gtgcgtgcac gttgccgacg cccgcgatgc tgaatgccca gccggccatg gtggccgcta
     1261 tctgcacctg ccccctcgcc gcgaggtcgt cgccctttgc cacgcacacg ggaaggtacc
     1321 tcgagagaag cctgatggcg tgcatggcca gcccgtcggt gatcggctcg tgcgggatcg
     1381 cgtggatggc ctccacggca tgggtcatgg cgtccatgcc ggtgcctgcc gtcaacaggg
     1441 gcggcagctt ggcggtcagc ttcgggtcca ggatgccgat gcgtggtatg ttgtagttct
     1501 cgaccatgag gcgcttctgc cccatctcct tgtccatgat gaccgcggcg ttggtcacct
     1561 cgctgcccgt accggcggtc gtcggtatgg cgatgtgggg ggtctggggc ctggacagca
     1621 gctggacccc gctgtaatcc ctgagggagc cgccctcggt gagcaggatg cacaccccct
     1681 tggccgtgtc gatcacgctg ccgccgccta tgctgatcac ggtgtcggct ccgcccttgc
     1741 gcgcgaagtc cgcgcaggcg tccaccacct ccacccccgt gtcctggggg acgtcgctga
     1801 agacgcccac gcacttgttg ccgagcgcct tgatgatgtg gtccgtgagc ccggccttga
     1861 tgatgccctg gtcagtgacc acgacggccc tggtgccgcc gatggaaccc atctcgatct
     1921 ccacgtcctt cgacgagtcc acgccgtaga tgaccttggt gggcccatag taattgaaag
     1981 acaggtcggt atcgtatgtc attgcccgct ccttcgctgt gttggactca aggcgcaccg
     2041 cgccggctcg acgcgcgagc ccgcgcctcc gcctgggtac gctacgcctc gtccggccac
     2101 agggtgtcca ccctgcccag gatgtcgatg accttgttga ccaccttgat gttcctgacc
     2161 acgtagggca ggtcgccctt catcttgagc ttgccctgga ggagcgcctt ggtcgagtcc
     2221 agctccttga gggcgacctg tttccagcgc gcatagtcgc cggtcatgac gaacttcgcg
     2281 ctctcggcct tggccttgtc gcacaccacg aactcgccca cgtccccgtg ccagtagtcc
     2341 atgaacaggt acacgggctc gggcacgccc ttgtccgggt cggggttgac gatgagcgcg
     2401 acgctgcctt cccagttctt tgcgatctcc ttgtactccg ggtccgcctc gatcgccgcc
     2461 ttgtaggcat ccatccattc ctgagtcata gccttgatct ttcccatgaa gagcacctcc
     2521 cctcgtttgt gtgtatgtgt tattggtaga gtcgcctctt gagttccttc aggccgagct
     2581 cgtccagctt ctcgggtttc gggaacccgc gctcgtcgag cccgcgaagc gcatagtact
     2641 ccttgaggag cagatccatg tccgggaccg agccggcggc cgccccctcg gaaagggccg
     2701 tgaggatccg cttcgggagc acgtcgtcct tccgggagat cccgagcagg ttgttgatgc
     2761 cgcgcttgag ggtccagtcg cgcatgccgg ccttgagcag gtcgtccttg ccgaggccga
     2821 acccggtcac cgcgttgaac gcctcgagga cggtctctgg cctgaggatg tacaccacgt
     2881 agtggcagat ggacagcgag ttgcacagga tgccgtagtt ctcggagttg aacaccagca
     2941 tggccttgcc ctcgctcgtg gtccccacgt agtcgtcttc catgtcgaag agctccggcc
     3001 acgaggccat gccctgctcc gtggcgagca cggcgtgctg gagatggcac gcgccgcggt
     3061 tcgacatcat gtaggcgagc cccatgccgt ggaaccccct cgggtcgtgc atgggggcct
     3121 cgagcccctt gacgtgcgcc accatgtcct cggctcccct gcctatcctg cgggcggcgc
     3181 cgagggtccc ctcggccagg atgtcgccga acccctccct gaacgcaatg ttcttgatga
     3241 gcggcatcac cgcgtccatg ttgccccagg tgagctcgag gccgccggtg tcgctcggcg
     3301 tcacgatccc cttctcgtag agctccatgg cgagagcgat cgccgaaccg cagctgatgg
     3361 tgtccatgcc gtagcggttg cagagctcgt tcgccttgat cacggccgcg agatcgtcgt
     3421 tcatgagcat ggtcccgaag gtcgcgcagg tctcgtactc ggggcccggc ccctcctcgg
     3481 tgcggtaggg gccgtcggcg accttgacca cccgcttgca ggcgaccggg cagttcgcgc
     3541 acgcgtgctc cttggtgagg tacttctccc gcatggtggg acccgagagg ttcgaagcga
     3601 ggtcgaaggc ctcgcccacg gtccagttct tgatcgggac gtcgcccttg atcatgccca
     3661 gttccatgtt cgcgtccgac cccatgaggt ggagaccctc ggccagtgcc gaatccttca
     3721 cctcggcgag ggccttcttc atcacctcct tgaagcgctc ttcgtcggcc tttgcgggtc
     3781 tccccttgcc gcgcgccacg atcgccttga gccgcttgga gcccatgacc gcgccgaggc
     3841 ccgtcctgcc gctgaagtgc cccttgtcgt tgccgatggc cgctatcttc acgaggttct
     3901 cgccggcctg gccgatggca agcaccttga gaccgccgtc ttttgccttg agctcgtccg
     3961 tggtctcgta gatgtccttc ccccacaggt gcttggcgtc atggatgccg gccttcccgt
     4021 cgaccaccga gaggtagacg ggcgatgacg acacgccggt cacgatgatt ccgtcgtagc
     4081 ccgcccgctt gagttccggg ccgaaggtac cgccgcaggc cgactcgccc cagatcccgg
     4141 tctgcggggc ccgtgcgcag atgctgaagc gcgaggtgcc cggaaagccg ctcgcgacca
     4201 tgggcccggt catgatcatg agcgggttgt cccccgagag ggggtcggcg tccagcttga
     4261 agcgatcgag gagcagcctc gccgcgagcg agctgccccc tatgaagtcc ttcagcaggg
     4321 tctcgtcgac tttcagctcg tctgtggttc tgttcgtaag gtccaccagg agaatcgcgc
     4381 cggcataccc gttgggcata gtgatacctc ctcgcagcat gtggtagaaa tacaatatta
     4441 gcagcataca agggtgatgc aatagggtgg catgcgaaga tggtgtggcc ggtcgcccga
     4501 gaccgccgta gcaggacatg ctacacggcc tcgacggcgc ccggttcccg gagtgacggc
     4561 tccggccctc cctcagccgt caacccagtg cacggcgtat ctcacgagtt ccgcggcgtt
     4621 cttgaggttg agcttctcct tgatgcgctc acggtaggtg ccgatggtgc tcaccgagag
     4681 gttcatggcc gatgcgatct cggaggtgga ctttccctg
//
LOCUS       DAOSCR010000143         4708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11474_length_4708_cov_4.028584, whole genome shotgun sequence.
ACCESSION   DAOSCR010000143 DAOSCR010000000
VERSION     DAOSCR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11474_length_4708_cov_4.028584"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1740)
                     /locus_tag="PLT69_04850"
     CDS             complement(280..1740)
                     /locus_tag="PLT69_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13803.1"
                     /translation="MGNGWMRDLAFHYESIRDTHPLSEIMIVFDIDGTVLDERYLVLS
                     VLRSYDRRHSTSCFENLRAEDITSSPGELDSMLKDFGIGPGARRDILAWFFENLFTAD
                     SVKAAQVPCKGVLDVIRWFQLQERTVVGLNTSRPEALRRETLEILNGLGSRYRVGFES
                     ALMFMNQGMGAREAKVEGIRFFQSLGYVVAAVIDSDTANLEALQRYEGSRDILLVHSD
                     LMWDRPHAEEARPWHPGNPCDITDLVREEDLPSSVEFAWHGVDEESVLRQFLVSPVNW
                     AELHVRRHPTSGRLVLRRRSYAEVPLLPHEHCPELDDFLYLIFEAGRGVKLDLKDPGL
                     APPVVASLASMGYTEDRVWFSLSIEDAMRGGLRAICEAFPGAVKQCPVDPLWRSIMFA
                     PHDARRRLHALSEAGIDRYSVDWNTPGSREIVVSLQNWGYEVNIYNVPDLESFLQAVL
                     LLPASVTSYFNFPKWFYTGCADDFPQAYPRLAAMSA"
     gene            complement(1876..2124)
                     /locus_tag="PLT69_04855"
     CDS             complement(1876..2124)
                     /locus_tag="PLT69_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13804.1"
                     /translation="MKPRTDRAWVFRETGRILKGLYGIWVTRDTYGYNSTLERFFRYL
                     TGRMARPEKGNPPAGRAAGRGVVPARDLGPGGFVGGGS"
     gene            complement(2642..3556)
                     /locus_tag="PLT69_04860"
     CDS             complement(2642..3556)
                     /locus_tag="PLT69_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOJ13805.1"
                     /translation="MRRDFELEGNRIVSCAEGEGSVTIFVNPDPEERKYLITGLGVDE
                     HTLASSLDPDELSRLEFEPDHVALIFKMPKSYSGEDQLQFRVCSTGAFLFKDRLVLVM
                     SEEVPLFEGKQFLKVSSLNSLFLKVINRSAHHFMEHLRVINLLTEELEDRINTSMENT
                     YLINLFTLGKSLVYYLNAINSNAIVIAKLKNNVQKIGLNTEEAEFLDDIMVDNNQCYR
                     QAEIYSNILSNLMDARVSIVNNNLNILMKTLNIIILCIMLPQFVVSAFSMNVAMPMQH
                     HPLAFWFIMGISLLSTLVVVVFWKYKKL"
     gene            4133..>4708
                     /locus_tag="PLT69_04865"
     CDS             4133..>4708
                     /locus_tag="PLT69_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13806.1"
                     /translation="MFPWKSAFSAVMVCLGALLVLLPLNLAAPPGDDEAWVWRAAGEM
                     REAGSFLPLLNGRVVGGANPLTMLGACAVPSTGDVPPRLINGALGCILAVGTFLYAHR
                     LFGLKSALASSVALLTTLGFQSTYGTLNLAAIPVTFSACAFGLFSLAYLERLPRWCYH
                     ASYVTAALAFITGGAPPLVFFAAASALTTSHT"
BASE COUNT          844 a         1551 c         1412 g          901 t
ORIGIN      
        1 agtcgacggc gacgacgtcg tcaaaccgct tcgtcacctc cttcagtgaa acaatgggct
       61 gcctctccat ccatgcggcc tccgtggcgt agtgatgtct tggagacacc ctgtctttat
      121 accaaaccac cggctcccgc aacggtattc ttgtctccga accctacttt tatcgtcctg
      181 gcggtacaac aatagtaaac ccgcaggggg gctcggtccc gggaggcgaa ccccgggaac
      241 gccgcccaga ggcttcccgg agccacacgg ctcggccgct cacgcgctca tggccgcaag
      301 gcgcgggtat gcttgcggga aatcatcggc gcacccggta tagaaccact tcggaaagtt
      361 gaagtaggag gtgacggatg cgggcaggag gagcacggcc tggagaaagg attccaggtc
      421 cgggacgttg tagatgttca cctcgtaccc ccagttctgc aggctgacga cgatctccct
      481 cgagccgggc gtgttccagt cgaccgagta ccggtcgatc cccgcctcgg agagcgcatg
      541 gaggcggcgc ctggcgtcgt ggggcgcaaa catgatggat ctccacaggg ggtccacggg
      601 gcactgtttg acagcgcccg ggaaggcctc gcagatcgcc ctcagtccgc ccctcatggc
      661 atcctcgatg ctcagggaga accacaccct gtcttctgtg taccccatcg acgcgaggga
      721 agccacgacg ggcggcgcca ggccggggtc cttcaggtcg agcttcaccc cccgcccggc
      781 ctcgaagatg aggtacagga aatcgtccag ctccgggcag tgctcatgcg gcagcaaagg
      841 cacctcggcg tacgagcgtc tcctgagcac caaccgaccg ctcgtggggt gcctgcgcac
      901 atggagctcg gcccagttca cgggagagac caggaactgc ctcagcacgg actcttcatc
      961 gacaccgtgc caggcgaact ccacgctcga agggaggtct tcctcgcgca cgagatcggt
     1021 gatgtcgcat gggtttcccg ggtgccaggg ccgggcctcc tcggcgtgcg ggcgatccca
     1081 catcaggtcc gagtggacga ggaggatgtc gcgggatccc tcgtaccgct ggagagcctc
     1141 gaggttggcc gtgtcgctgt ctatcaccgc ggcgacgacg tacccgagag actggaagaa
     1201 cctgatgccc tccaccttgg cttcgcgcgc gcccatccct tggttcatga acataagagc
     1261 gctttcgaac cccacgcggt acctcgatcc cagcccgttg aggatctcga gcgtctcgcg
     1321 tctcaacgcc tcgggacggg aggtgttcag tcccaccacc gtcctctcct gcaactggaa
     1381 ccacctgatg acgtcgagca cccccttgca cggcacctgg gcggccttca ccgagtccgc
     1441 cgtaaagagg ttctcgaaga accaggccag gatatcccgc ctcgcgccgg ggcctatgcc
     1501 gaaatccttg agcatcgagt cgagctctcc gggcgaagag gtgatgtcct cggcccgaag
     1561 gttctcgaaa cacgacgtcg agtgccttct gtcatacgac ctgagcaccg acaggaccag
     1621 gtaccgttcg tcgaggacgg tcccgtcgat atcgaacacg atcatgatct ctgacagggg
     1681 gtgcgtgtcc cgtatggatt cgtagtgaaa cgccagatcc ctcatccacc cgttgcccac
     1741 gatccggtca ctcctttggg tcgagccctc ctatgcggcc ctgagggcat gattccaacg
     1801 cccggttcac caccttggtg cactccggtt gaacctcgag aggtcgatcc ttgcaccagg
     1861 ctcttcgtgt caggctcacg atccaccccc cacgaaacca cccggcccca agtctctcgc
     1921 ggggacgaca ccccgaccag cggcacgccc ggcagggggg tttcctttct cggggcgggc
     1981 catcctgccg gtgagatacc tgaagaacct ctccagggtg gaattgtagc cgtaggtatc
     2041 cctggtgacc cagatcccgt acagcccctt cagtatccgc ccggtctcgc ggaacaccca
     2101 tgcacggtca gtgcgcggtt tcatgatcaa gacctcccgc gtatgcttat cggcagatta
     2161 cactgtcatt atgaatgaat gatgaatgca gggtgcccat ggcgaacatc cacggctgac
     2221 acaagcggcg acccgtggag cacggggttc cacgggtgat ggttcagcga gctcctatcc
     2281 gcgttttctg gctccctcaa aagatccacc catatcatcg ggctcacgcg gacagaccga
     2341 ggccctcgcc cttgatcccc ccatcccttg ccgggaaagc attgcaccgc gcccgacgag
     2401 catcatcaca cctcgatctc aaggttcttc tcatgccctc agcgagggcc cgtggcgctc
     2461 cctgggagga gaccgtcacg gtcccggctc acaacgtctt gccggtgcgc gggaaagggg
     2521 cggggggaag tgcagcggcc cgccatgtcg ggagaagacc ggtatcggcc ccaaggggca
     2581 gacacgggag tcgggccggc acggcggggc ggctcgccca ttcggacgcg cctaagcgct
     2641 gtcacaattt cttgtacttc cagaagacca cgaccacgag ggtcgacaac agcgagatcc
     2701 ccatgatgaa ccagaacgcc agggggtggt gttgcatggg catggccacg ttcatggaaa
     2761 aggcgctcac cacgaactgg gggagcatga tgcacaggat gatgatgttg agcgtcttca
     2821 tgagtatgtt gaggttgttg ttcacgatcg acacccgcgc gtccatgagg ttcgaaagga
     2881 tgttcgagta gatctccgcc tggcggtagc actggttgtt gtcgaccatg atgtcgtcga
     2941 ggaattccgc ctcctcggtg ttcaggccta tcttctggac gttgttcttg agcttcgcga
     3001 tgacgatggc gttcgagttg atggcgttca ggtagtaaac caggctcttc cccagggtga
     3061 agaggttgat gaggtacgtg ttctccatcg aggtgttgat gcggtcctcg agctcttcgg
     3121 tgagtaggtt tatcaccctg aggtgttcca tgaagtggtg cgccgatcgg ttgatcacct
     3181 tgaggaaaag gctgttgagc gaggacacct tgaggaactg cttcccctcg aacagcggca
     3241 cctcctccga catcacgagc acgagcctgt ccttgaagag gaaggcgccg gtggagcaga
     3301 ccctgaactg gagctggtcc tcaccggaat agctcttggg catcttgaag atgagggcga
     3361 cgtgatccgg ctcgaactcc agcctcgaga gctcgtcggg gtcgagcgag gacgcgagcg
     3421 tgtgctcgtc caccccgagc ccggtgatga ggtacttccg ctcctcggga tccgggttga
     3481 cgaagatcgt cacactccct tcaccctcgg cgcaactcac gatcctgttg ccttcgagtt
     3541 cgaagtctct ccgcatgcgt tcttcccgtt gcgacgaccg gcgcagcgga accggaagag
     3601 atctctgaag tcctggatcc ggcgcggcca tccttaccca cccagggcag gatgacctgc
     3661 tcctaggcgg gattacctcg gtcgtttacc ggtgatgtcg tcggtactca ttggcaacgt
     3721 gcctagcaca catttcttcc atgcgtcaaa caaaagatcc tgccctgtct ctgtccaggc
     3781 acaaacgcat ccccgcctcg cccggccgtc cccgtgccca gcacgtggat cccctttctt
     3841 cccgcctgga cagccctggc gggacgggct tctcctcaag ggctgcacga cggccgtctc
     3901 gaccctcgcg cgcggctgca ttggggacac cgcatcgtac gagctcatgc cgcatgcgcc
     3961 gccctcgcac ttccgaagga acgtgggggt gccggccgtg ggatacccgg atgcctcacc
     4021 cccaggacag gggccgtatc tcctcgtaaa aacggccggg aaaggacatc ttgcgacttg
     4081 acaccctgtt tccggctcat gtaatcgctc agggcatagg gaggtatgcc ccgtgttccc
     4141 ctggaaatcg gccttctcgg ccgtgatggt ctgcctgggc gctctcctgg tgctcctgcc
     4201 cctgaacctg gcggcgcctc caggagacga cgaggcctgg gtctggcgtg cggccgggga
     4261 gatgcgggag gcaggctcgt tcctccccct gctcaacggc cgggtagtgg ggggcgccaa
     4321 ccccctgacc atgctcgggg cctgcgccgt cccatccacg ggtgacgtcc cgccgaggct
     4381 catcaacggg gcgctcggct gcatcctcgc cgtcgggacg ttcctctacg cgcaccgact
     4441 cttcggcctg aagagcgccc tcgcatccag tgtcgccctg ctcaccaccc tggggttcca
     4501 gtccacctac ggcaccctca accttgccgc cataccggtg acgttttccg catgcgcatt
     4561 cgggctcttt tccctcgcct acctcgagag gctgccgcgc tggtgctacc acgcctcgta
     4621 cgtcacggcc gccctggcct tcatcacggg tggggcgccg cccctggtct tcttcgccgc
     4681 ggcatccgcg ctcacgacgt cccacacc
//
LOCUS       DAOSCR010000144         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11526_length_4692_cov_3.846452, whole genome shotgun sequence.
ACCESSION   DAOSCR010000144 DAOSCR010000000
VERSION     DAOSCR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11526_length_4692_cov_3.846452"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLT69_04870"
     CDS             complement(<1..603)
                     /locus_tag="PLT69_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOJ13807.1"
                     /translation="MIVYNSMQASDKPLIIQSDGSVLLEVHSEAYDEAKDAIAPFAEL
                     VKSPEHVHTYRITPLSLWNASAMGVTREEVMAALERFSRYEIPHTVARRIGEAFERWD
                     AVRLFEHDGDTLVARIKNGEILSLLRTSAEVSKMIVGVDEPLGLVMPRGVRGRFKHAC
                     IKMGYPVNDLVGYEPGDPLPMDLRPVTSSGEQFRLRPYQEE"
     gene            834..2060
                     /locus_tag="PLT69_04875"
     CDS             834..2060
                     /locus_tag="PLT69_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ13808.1"
                     /translation="MVFNEVNPGGITRADIVVGIPSCNEAAAIALPAEQAALGLREHF
                     ANLRSVIINCDNSSTDGTPQAFMEAPDHGVPKMVISTPRGIRGKGNNLKNLFRKAVDL
                     EAKAVVVLEANLKSVGPVWVKNLAEPVFKGFSFVSPLYVRHKYESPVATTIAYPLTRC
                     LYGRRVRQPIGGDCAFSGDMAHTFLKSTLWDEDVSQFGIDLWMCTLAVNEGVPICQAF
                     LGRPKISTTKPRDPALDMGPMFRNMVVTLFNLMGHFKDKWATTKWSKPTAIFGFNLKD
                     IEPPPPITVNRERLYTKFRDGFNSQWDLYRALLAGENLQKIREIASLDLDHFEIPVTV
                     WARILFDCAIGYHRRVMEPARILDVLASLHFGMILSYVNKTEGMSHQQAEEYLEDMCL
                     VFEQNKPYLTYRWDQK"
     gene            2086..2451
                     /locus_tag="PLT69_04880"
     CDS             2086..2451
                     /locus_tag="PLT69_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ13809.1"
                     /translation="MPKILIVDDEKHIRLLYTEELEEEGYEVATASDGVGILDRIAEE
                     KPDVVILDIKMVSSNGLDVLQEIRNTYYNLPVILCSAYGSYKVDLKSIAADAYVVKSS
                     DMTELKKKIAQVLETNVPA"
     gene            2459..3292
                     /locus_tag="PLT69_04885"
     CDS             2459..3292
                     /locus_tag="PLT69_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ13810.1"
                     /translation="MRVAADLHVHSNASDGAHSPTEVVQRARSSGIVAVAITDHDTLG
                     GIGEALSASRGTSLSVVAGTELSARSPRGTLHILGYFPCWPSGLEAELASLERARRER
                     IPRIVDRLRGLGMDITEQDVETYAARAVPGRPHVARVLVDKGFASSVDDAFHRFLARG
                     RPGYVPKAKPSVRDALALVRDHGGIPVLAHPSSLGMDRGELRRFVRFLARSGLEGIEA
                     FHPDHGPGETAHCIGMAEALGLLVTGGSDYHGLEDHECPIGAVGIDESRLEAFLARLG
                     G"
     gene            3996..>4692
                     /locus_tag="PLT69_04890"
     CDS             3996..>4692
                     /locus_tag="PLT69_04890"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOJ13811.1"
                     /translation="MFSKNVLDQIGNTPIVCLKNALNANIFAKAEHLNPGGSIKDRVA
                     LFMLEEARRKGVLKPGAVIAEPTSGNTGIGMALVGGIMGHRVICVMPENMSEERKKII
                     RALGAELILTPAEESLEGCIRVIDDLRRDIKNLYVPQQFENPNNPLAHYLSTGPEIWS
                     QLRHHVDVFVSGLGSGGTLQGVGSFLKERNKDTVVVAVEPKNVSALLGHEPGLHRIEG
                     IGDGFIPPVLDTSI"
BASE COUNT          883 a         1570 c         1499 g          740 t
ORIGIN      
        1 ctcctcctgg tacggcctca gcctgaactg ctcgcctgag gaagtgaccg gcctgaggtc
       61 catggggaga gggtcgcctg gctcataccc cacgaggtcg ttcaccgggt agcccatctt
      121 gatgcaggcg tgcttgaacc tcccgcggac ccccctgggc atgaccagcc ccagcggctc
      181 gtccacgccc acgatcatct tggagacctc ggcggatgtt ctcaagagag acaggatctc
      241 cccgttcttg atccttgcca ccagggtgtc cccgtcgtgc tcgaagaggc gcacggcgtc
      301 ccaccgttcg aaggcctccc cgatgcgcct ggccacggta tgcggtatct cgtagcgcga
      361 gaacctctcg agcgccgcca tgacctcttc cctggtcacc cccatggccg aggcgttcca
      421 caaggagagg ggggttatcc ggtaggtatg gacatgctcg ggggacttca cgagctccgc
      481 gaagggcgct atcgcatcct tcgcctcgtc gtacgcctcg gaatgcacct cgaggaggac
      541 gctcccgtca ctctgtatga tgagcggctt gtcgcttgcc tgcatcgaat tataaacgat
      601 cacacagcac cctcgaaaag gcaaggggag aattggcgac ccaggggctc aaacccggga
      661 cctcggcggc ctttccgcgg cggcctcgac cacgttctca ccttttgcgc ccgctcctct
      721 ctttccgttg cgtaaggggc tgaaggtttt tccagttgcc gcacggcgat aaatgtccca
      781 atatatgaag gcggggaggc aggcccgtcc cctagacaag gagccaggtc ggcatggttt
      841 tcaacgaggt gaaccccggc ggcataaccc gggcggacat agtggtgggc atcccgtcct
      901 gcaacgaggc ggcggccata gccctgcccg cggagcaggc ggccctgggg ctcagggaac
      961 acttcgcaaa cctcaggtcc gtgatcatca actgcgacaa cagcagcacc gacggtacgc
     1021 cccaggcctt catggaggcg ccggatcacg gcgttcccaa gatggtgatc tccacgccga
     1081 gggggatcag gggcaagggc aacaacctca agaacctctt ccgcaaggcg gtggacctcg
     1141 aggcgaaggc ggtggtggtg ctcgaggcca acctcaagag cgtgggccct gtctgggtca
     1201 agaatctggc cgaacccgtg ttcaaggggt tttccttcgt gtcacccctg tacgtgaggc
     1261 acaagtacga gagcccggtc gccaccacta tcgcctaccc gctcacgcgc tgtctctacg
     1321 ggaggcgtgt ccgccagccc ataggagggg actgcgcctt ctccggcgac atggcccaca
     1381 ccttcctcaa gagcacgctt tgggacgagg acgtgagcca gttcggcata gacctctgga
     1441 tgtgcacgct cgcggtgaac gagggggtcc ccatctgcca ggcctttctc gggaggccca
     1501 agatcagcac caccaagccc agggacccgg cgctcgacat ggggcccatg ttcaggaaca
     1561 tggtcgtcac cctcttcaac ctcatgggtc acttcaagga caagtgggcc accaccaagt
     1621 ggagcaagcc cacggccatc ttcgggttca acctcaagga catcgagccc cccccgccca
     1681 tcaccgtgaa cagggagcgg ctctacacga agttcaggga cgggttcaac tcgcagtggg
     1741 acctctaccg cgccctgctg gcgggcgaga acctccagaa gatccgggag atcgcctcgc
     1801 tcgacctgga ccacttcgag atcccggtga cggtctgggc gaggatcctg ttcgactgcg
     1861 ccatcgggta ccacaggcgc gtgatggaac cggcgcggat actggacgtc ctggcgtcgc
     1921 tgcacttcgg catgatcctg tcgtacgtga acaagaccga gggcatgtcg caccagcagg
     1981 ctgaggagta cctcgaggac atgtgcctcg tcttcgagca gaacaagccg tatctcacct
     2041 accggtggga ccagaagtaa cactagcaac cccaaggagg tgaccatgcc caagatcctc
     2101 atcgtggacg acgaaaagca cattcgtctc ctctacacgg aggagctgga ggaggagggg
     2161 tacgaagtcg ccaccgcgtc ggacggcgtg gggatcctgg ataggatagc cgaggagaag
     2221 ccggacgtgg tgatcctgga catcaagatg gtctcctcca acgggctgga cgtgctccaa
     2281 gagatccgca acacctacta caacctcccc gtgatcctct gcagcgcgta cgggagctac
     2341 aaggtcgacc tgaagtccat agcggcggat gcgtacgtgg tgaagtcctc ggacatgacc
     2401 gagctcaaga agaagatcgc gcaggtcctg gagacgaacg tccccgcgtg aagactgcat
     2461 gcgcgtcgcg gccgacctcc acgtgcactc gaacgcatcc gacggcgcgc acagcccgac
     2521 ggaagtggtc caaagagccc ggtcctcggg catcgtcgcg gtcgcgatca cggaccacga
     2581 caccctgggc ggcatcggcg aggcgctctc ggcatctcgg ggcacctccc tctccgtggt
     2641 cgcgggcacg gagctgagcg cccgatcgcc acggggcacc ctccacatcc tcgggtactt
     2701 tccctgctgg ccctcgggcc tcgaagccga gctcgcatcc ctcgagaggg cccgcaggga
     2761 gaggatcccc aggatcgtcg acaggctcag aggcctcggg atggacataa ccgagcaaga
     2821 cgtggaaacg tacgccgccc gcgcggtccc cgggaggcca cacgtcgccc gggtcctcgt
     2881 cgacaagggg ttcgcatcga gcgtcgacga tgcgttccac aggtttctcg cgcggggaag
     2941 gcccggttac gtccccaagg ccaagccgtc ggtacgcgac gccctggccc tcgtcaggga
     3001 tcacggcggc atacccgtgc tcgcccaccc gtcctccctc gggatggacc gcggcgaact
     3061 gaggaggttc gtgcgcttcc tcgcgcgctc gggcctcgag gggatcgagg cctttcaccc
     3121 cgatcacggg ccgggcgaga ccgcgcactg catcggcatg gcggaggccc tggggctctt
     3181 ggtcacaggc gggagcgact accacggcct ggaagaccat gagtgcccca tcggcgcagt
     3241 gggcatcgac gagtcgaggc tcgaggcctt cctggcccgc ctcggtggtt gaagcatcgc
     3301 ctcgccgtga ggccgggccg cggggccgcc ccctgcacgg aggccgcgcg tgtcgaggga
     3361 cgcgctttgc cgcctggatc cgctcccgag ggccgtcacg cacagtcgat gtcgtggggg
     3421 caacgtgccc gccggaaggg cgcacgggtg ggccgacgtc ttcggcgcga gccacggcgc
     3481 cgccttgaga ccggcctatg acacacgacc cggaaggcat gccgcaccgt gggcggcatc
     3541 accggcccgt gaggcggcga agacgccccg agacgtcgtc cgggcgtctc ggcctctccg
     3601 ccagcatggc ccgaaccgaa gtcccggcgc cttccacccg aaaacccgca acgcaaggcc
     3661 tcctgcagcc cgccatgatc cgggacccct tctccacgca tcgcgacggc cctcgaggtc
     3721 acgccgcggc acacccccag ggagagcctg cctcgcgggg tcatacccgg gtgcatcccg
     3781 cccgcagggt ggaagcacgt cccgagacgc cggggagggc atcgcggcga cccttgcggc
     3841 cttcaggccg caaggaccct ggagcggacc cgtttgatct tgacacgggg cgccttctcg
     3901 tctatcgggt agggctgtac caagggcgca acaggtgcgg ggccctcctg ccgccaaagg
     3961 atccgcatcg agagaccact cgggagcact ggagcatgtt ttccaagaac gtcctcgacc
     4021 agatcggaaa cacccccatc gtgtgtctga agaacgcctt gaacgccaac atcttcgcca
     4081 aggcggaaca cctcaatcca ggcggcagca tcaaggaccg cgtcgccctg ttcatgttgg
     4141 aggaggcgag gaggaagggc gtgctcaagc ccggcgccgt gatcgcggag ccgacctcgg
     4201 gcaacaccgg gatcggcatg gcgctcgtgg gcgggatcat gggccatcgg gtcatatgcg
     4261 tcatgcccga aaacatgagc gaggagcgaa agaagatcat cagggcgctc ggggcggaac
     4321 tcatcctcac ccctgcagag gagagcctcg aggggtgcat ccgggtgatc gacgacctgc
     4381 gcagggacat caagaacctc tacgtgccgc agcagttcga gaaccccaac aaccccctcg
     4441 cccactacct ctcgacgggg ccggagatct ggtcgcagct caggcatcac gtggacgtct
     4501 tcgtctccgg gctcggaagc ggcgggacgc tgcagggagt cggctccttc ctcaaggagc
     4561 gcaacaagga taccgtcgtc gtggcggtgg aaccgaagaa cgtgtccgcg ctcctgggcc
     4621 acgaacccgg cctccaccgg atcgagggta tcggggacgg gtttatcccg cctgtgctgg
     4681 acacctccat cg
//
LOCUS       DAOSCR010000145         4664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11610_length_4664_cov_3.306140, whole genome shotgun sequence.
ACCESSION   DAOSCR010000145 DAOSCR010000000
VERSION     DAOSCR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11610_length_4664_cov_3.306140"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLT69_04895"
     CDS             complement(<1..657)
                     /locus_tag="PLT69_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13812.1"
                     /translation="MSMRDRPRLGRGLKDVSRYFLADSHPRGGPDPVPTSEDARPISV
                     FVFHASSPEVQFLLTLALAMETARLGRGALIWDGMAQGAQGVKRLAGGLLARPGGRDK
                     GGGCCIRLYGLGAIPIHDAACPVWSQAEASCLDLPGDGDTARGELLVFVNASPDMGFF
                     TRGDLPDHTVIAARTDERSLLKAYACMRVMDRRAPGSRMYLVLEAHGGEPANQCDRTF
                     E"
     gene            complement(654..1724)
                     /locus_tag="PLT69_04900"
     CDS             complement(654..1724)
                     /locus_tag="PLT69_04900"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13813.1"
                     /translation="MRIIAVINQKGGCGKTTTAINLGACLAGLSRRVLVVDLDPQAHA
                     TLGFGVDQGEFRLSSYDLLMEGRDGLLSSDMIISLNERLDLIPSDVMLSAAEPVLLQR
                     DGREHVLESVLAPLDGRYDFVVLDCPPNIGVLTFNALYACTEAIIPMESGLFALHGLA
                     KLLETIDVVNGNREVPIEVHALATMFDRRTRIAHESLEELRKHMRGRVFRTVVNFNVK
                     LKEASGYGMHILEYDPDSQGAKDYLALAREVLSARPSARKRRKKEGFLKPVVTEDGVL
                     FTYYSPEATSVHVVADFNGWDTSGLPMENVAGTGMWQRFVPLKKGRYEYKFLVDGRWV
                     SDPDNPRRAANEFGENSILEIA"
     gene            complement(1948..2472)
                     /locus_tag="PLT69_04905"
     CDS             complement(1948..2472)
                     /locus_tag="PLT69_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOJ13814.1"
                     /translation="MESRRSETLVMALSNERRERAFYLAQAERTRNRLGRSMFLRIAD
                     DELEHAVRLERIHEELARGGAWPEGVELGLKLGEVGRSLDALVGELGQAAGEADADDL
                     EAVETACRFEAEGERLYAQLAGAASEKREKAFFELLSSMEREHFRALKEVEEYLRDPA
                     SYFTVKERHGLDGA"
     gene            complement(2576..3616)
                     /locus_tag="PLT69_04910"
     CDS             complement(2576..3616)
                     /locus_tag="PLT69_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ13815.1"
                     /translation="MKVKRLFLAEAIVALALFASATGAFAENREGAVNVTPWIGGYNL
                     DGDLPFDNGTVFGLGLGYNFTEKIGAELSLYYVETDYDGPPLIDGGIDFNDDDASIYL
                     GKLDALYHITGMLPDMVVPYLAAGIGVNTYDSDMKGVDSDTDLILNYGAGLKFFLKRD
                     IALRADARHVLDFDGGDTYNNFLYTFGLCFEFGGKTKEAEVVTVEPEPAPAPAPAPAP
                     EPAPAVAPTPPPVEKQGAIVFRNIQFDLNKATLKEESYPILDEVADYLKANPSVKMEV
                     QGHTCNLGKAEYNLKLSDQRAKAVKDYLVGKGIAADRLDAKGYGLTKPAFPNDSEENR
                     SKNRRVEFKPIQ"
     gene            complement(3770..4315)
                     /locus_tag="PLT69_04915"
     CDS             complement(3770..4315)
                     /locus_tag="PLT69_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13816.1"
                     /translation="MRGVQAVAARQEARVPREMRSHPDLPKVVEALSRSYRPLWRESS
                     VEVPVVPFSEGLAAALRRASQKGFLVRGLETASEVLGNEKRGIDRSSSEGGSSAATGI
                     SRLIVAATDGSERFYRAVESLLVAHRPRVLAVLAEADAYRFGEEVFRTGRLCRLVLVT
                     RRKAVADVLLALAHAETACGG"
     gene            4491..>4664
                     /locus_tag="PLT69_04920"
     CDS             4491..>4664
                     /locus_tag="PLT69_04920"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOJ13817.1"
                     /translation="MDLARTEPRTLISMLLATFFGAGFSPKAPGTVGTLAALPLYLAL
                     RRLSLPLYLAFAAK"
BASE COUNT          773 a         1611 c         1422 g          858 t
ORIGIN      
        1 ctcgaacgtc cggtcgcact ggttggccgg ctcccccccg tgggcctcga ggaccaggta
       61 catcctcgac ccaggggccc tcctgtccat caccctcatg cacgcgtagg ccttgagcag
      121 gctgcgctcg tcggtacggg ccgcgatgac cgtgtggtcg gggaggtcac cccgagtgaa
      181 gaaacccatg tcgggcgagg cgttgacgaa cacgaggagc tcccctcggg cggtatcgcc
      241 gtcaccgggc aggtcaaggc aactggcctc cgcctgcgac cagaccgggc aggccgcatc
      301 gtgtatcggg atcgcgccca ggccgtagag ccttatgcag cagcccccgc ccttatcccg
      361 gccgccgggc cgggccagga gcccccctgc caggcgcttg accccctgcg ccccttgggc
      421 cataccatcc catatgaggg cgcctcgccc gagcctggcg gtctccatgg cgagggcgag
      481 ggtgagcaga aactggacct cgggggacga cgcgtggaac acgaagacgc ttatcggccg
      541 ggcgtcctcg ctcgtaggta cggggtcggg accgccgcgg ggatgcgagt ccgcgaggaa
      601 gtacctcgac acgtctttca ggcccctgcc cagtctcggc ctgtcgcgca tgctcacgct
      661 atctcgagga tcgagttctc gccgaactcg ttcgcggccc ttctcgggtt gtcagggtcg
      721 ctgacccacc tgccgtcgac gaggaacttg tattcgtacc tgcccttctt caggggaacg
      781 aagcgttgcc acatgcccgt gcccgccacg ttctccatgg ggagacccga cgtgtcccag
      841 ccgttgaagt ccgcaaccac gtggaccgac gtggcctcgg gcgagtagta cgtgaagagc
      901 accccgtcct cggtgaccac cggcttgagg aacccctcct tcttcctcct cttgcgcgcc
      961 gagggccgtg ccgagaggac ctccctggcg agcgcgaggt agtccttcgc gccctgggag
     1021 tccgggtcgt actcgaggat gtgcatgccg tagccggagg cctccttgag cttcacgttg
     1081 aagttcacga ccgtcctgaa gacgcggccc ctcatgtgct tcctgagttc ctccagggac
     1141 tcgtgggcga tcctcgtcct cctgtcgaac atggtggcga gcgcgtgaac ctcgatggga
     1201 acctcccggt tgccgttgac cacatcgatg gtctcgagca gcttggccag cccgtgaagc
     1261 gcgaacaggc cgctctccat gggtatgatg gcctcggtgc acgcatacag ggcgttgaag
     1321 gtgagcacgc cgatgttcgg cgggcagtcc agcacgacga aatcgtagcg cccgtccagt
     1381 ggtgcgagga cggactccag cacgtgctcc ctcccgtcgc gctgcagcag tacgggttcg
     1441 gccgcgctga gcatgacgtc ggaggggatg aggtcgagac gctcgttcag gcttatgatc
     1501 atgtcagaag agagaagccc gtctcggcct tccatgagga gatcgtagct gctcaggcgg
     1561 aactccccct ggtcgacccc gaacccgagg gtggcgtggg cctgggggtc gaggtcgacc
     1621 accagaaccc tcctcgacaa accggcaagg cacgccccca ggttgatggc cgtggtcgtc
     1681 tttccgcacc cgcccttctg gttgatgaca gcgatgatcc tcatcaggca cctccccgca
     1741 catggtaatc cccatcctgc gcacaccccg tcgtggagca cgaggcgcga tcagctcatt
     1801 ccatcgtcca gcccccgggc atcgaaccag gccgggaaac ctctgcccgc ttgtccgacg
     1861 cgccgggaac agcactcttc gcctccatgc cttcaccggt ggcaagacca cggcaaggcc
     1921 tgcacgtcaa gagaatcact gttcttttca agccccgtcc aggccgtgac gctccttcac
     1981 ggtgaagtag gacgcggggt cccggagata ctcctccacc tccttcaggg cccggaagtg
     2041 ctcccgctcc atggacgaga ggagttcgaa gaaagctttc tctctcttct cggaggccgc
     2101 cccggcgagc tgggcataga gcctctcacc ctccgcctcg aacctgcacg cggtctcgac
     2161 ggcctcgagg tcgtccgcgt ccgcctcgcc tgcggcctga ccgagttcgc cgacgagggc
     2221 atcgagagac ctcccgacct cgcccagttt gagacccagc tcgacccctt caggccaggc
     2281 gccaccgcgg gcgagttctt cgtgtatcct ctccagccgc acggcgtgtt cgagctcgtc
     2341 gtccgcgatc ctcaggaaca tggaccggcc gagacggtta cgggtcctct cggcctgcgc
     2401 gaggtagaac gcacgttccc tgcgctcgtt ggagagcgcc atgacaagcg tctcggaccg
     2461 ccgactctcc atgtgcaccc ccttgccctc tcctgatgac acgggccgga gcccccgcac
     2521 ggaggcccgg cccacgtcgt ctgcagcctg aaccagatcg gaacgatgct tcctgttact
     2581 ggatgggctt gaactcgacc ctcctgttct tcgacctgtt ctcctcggag tcgttgggga
     2641 atgcgggctt cgtcaggccg taacccttcg catccagcct gtccgccgcg atacccttgc
     2701 cgaccaggta atccttcacg gccttggccc tctggtcgga gagcttgagg ttgtattcgg
     2761 ccttgccgag gttgcaggtg tggccctgga cttccatctt cacggaagga ttggccttca
     2821 ggtagtcagc gacctcgtcg aggatcgggt agctctcttc cttgagggtc gccttgttga
     2881 ggtcgaactg gatgttcctg aagacgatgg cgccctgctt ctccaccggg ggcggcgtgg
     2941 gggcgaccgc aggggcgggc tcgggcgcag gggccggggc gggcgcgggg gccggctcgg
     3001 gctccacggt caccacctct gcctccttgg tcttgccgcc gaattcgaag cacaggccga
     3061 acgtatagag gaagttgttg tacgtgtcgc cgccgtcgaa gtcgaggaca tggcgggcgt
     3121 cagccctgag cgcgatgtcc ctcttcagga aaaacttcag tccggcgccg tagttgagga
     3181 tgaggtccgt gtcagagtcc acgcccttca tgtcgctgtc gtacgtgttc acgccgatgc
     3241 ccgcggcgag atacgggacc accatgtccg gcagcatgcc ggtgatgtgg tacagggcat
     3301 cgagcttgcc gaggtagatc gaggcgtcgt cgtcattgaa gtcgatcccg ccgtcgatga
     3361 gaggggggcc gtcgtaatcg gtctccacat agtagaggga aagctcggcg ccgatcttct
     3421 cggtgaagtt gtacccgaga ccgagaccga acaccgtacc gttgtcaaag ggcagatcgc
     3481 cgtcgagatt gtagcctccg atccatggag tcacgttcac ggcgccttca cggttctccg
     3541 caaaggcacc cgtcgcgctc gcgaacaggg ctaatgccac gattgcttca gccaagaaga
     3601 gcctcttcac tttcataact acctcctcct cgttctctct gtgggaatat tcctgctggg
     3661 ggaacagttc acgtaaaatc aagataattc gtttcccccg ccttgtcaat ggttctcaac
     3721 ggatcgcccg tggaatgtcc gcgcccgagt cggcctgcag cccgcccgct caccctccac
     3781 acgccgtctc cgcatgagcc agtgcgagga ggacatcggc cacggccttc ctgcgggtga
     3841 cgagaacgag cctgcacaac ctccccgtcc tgaagacctc ctccccgaac ctgtaagcgt
     3901 cggcctcggc gaggacggcc aagaccctcg gccggtgggc gacgaggagg gactccaccg
     3961 ccctgtaaaa gcgctcggac ccgtcggtgg cggccacgat caggcgtgat ataccggttg
     4021 cggcgctgga ccccccttcc gaggacgaac gatcgatacc ccgcttctcg ttcccgagca
     4081 cctccgacgc ggtctcgagg cccctcacca gaaacccctt ctgcgacgcc cgtctcaagg
     4141 ctgcggcgag accttcagag aaaggcacca cgggcacctc gacagaggat tccctccaca
     4201 agggcctgta cgagcgagac agggcttcga ccaccttcgg gaggtccggg tgtgaacgca
     4261 tctccctggg gaccctcgct tcctgcctcg ccgccaccgc ctgcacccct ctcacgtgcc
     4321 ccaataggcc ctcgcgcgcc gaaggtcaag atcattcagg gcgtctcccc ggggtgtcga
     4381 ggcgcttgcc ccgagcattc accggcgagg tctcccttct ctcccgggcg cgatcgtgct
     4441 atcctctggc catgaccacg gcaggaggca agccatccac gtccacggtg atggacctcg
     4501 cccgcacgga acccaggacc ttgatctcca tgctcctcgc gaccttcttc ggggccgggt
     4561 tctcgccgaa ggcgcccggc accgtgggca ccctcgcggc attgcccctc tatctcgccc
     4621 tgaggagact cagcctcccg ctctacctcg ccttcgccgc gaag
//
LOCUS       DAOSCR010000146         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11650_length_4653_cov_3.541540, whole genome shotgun sequence.
ACCESSION   DAOSCR010000146 DAOSCR010000000
VERSION     DAOSCR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11650_length_4653_cov_3.541540"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLT69_04925"
     CDS             complement(<1..987)
                     /locus_tag="PLT69_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ13818.1"
                     /translation="MAVLLLAAYPALSAQQAPPKTVRIGNILPLTGPSSVAGKEAMCA
                     VEMATEEINSLGGIKSMGGARLENVWADSASDPTKGMSELARLVTEAKVHLVSGAWNS
                     AVTYPTSQAADTAGIPYVVPVSVRDSITERGLRYVFRLAPKDSWRSRDQFRFLADMKR
                     RSKAPVQSLAFVFENGPWGSSMRDQWQRLAASEGYRVALAESYPASSQDLSATAMKVA
                     NAKPDVILLASIGEDAQTLAKAFGDIKVKAAAVVASGGGHSDPSFIRKAGRACEYLFD
                     VSMWEPDMDRPSIARLRDAFGKRCKGALSAEAVYSYVSVFVIAQALEDAGKAT"
     assembly_gap    1677..1686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1687..3291)
                     /locus_tag="PLT69_04930"
     CDS             complement(<1687..3291)
                     /locus_tag="PLT69_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOJ13819.1"
                     /translation="MIQWQKSNDVLGTANRGNPAESGLCTLCRADCTGKCETWLSSLI
                     GRKLLYPRDFGIVTAGSSNTVHVGVSYNSLRIQGYNYGAYNRPKGLADSADDCVFPKV
                     SIETEFGSKVKTKSRIPVMTGALGSTFVAAKYWESFAVGASLVGIPIVVGENVVGVDK
                     ASVIKNGKISKAPELDRRIDTYLRYHDGYGAIIVQMNVEDTRNGVAEYVIDKYGDKVI
                     IELKWGQGAKDIGGEIQVTSLDYAIFLKKRGYVVDPDPTLPEVQEAFKHGAIRSFARH
                     SRLGYTNLGSPEEVQEDFMKEVNYLRKLGYSRISLKTGSYGMEALAMAIKYATEAKLD
                     LLTIDGSGGGTGMSPWNMMETWGVPSIYLHSKAYEYCSILAKKKKKVVDIAFAGGIAR
                     EDHIFKAIALGAPFTKLVCMGRAAMIPGFVGSNIEGALFPERREKVQGHWEKLPKTVT
                     DIGHKADEIFAGYYDVQKKVGAKEMENIPYGAIAMWTLCDKLSAGLQQLMAGARKFSL
                     SGVSREDLFSANKETVEVTGIPFITDV"
     gene            3527..4537
                     /locus_tag="PLT69_04935"
     CDS             3527..4537
                     /locus_tag="PLT69_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOJ13820.1"
                     /translation="MTEKKEKIRHAILSILRDAESPLSSYAISSQLIDRGIEISERTV
                     RLYLQVMDEEGLTMKSNRRCHAITEQGVQELGSWRTLERVGFLSARIDQMTYQMDFDL
                     ATGRGTVIVNITNVPPRAALEHAHSIKKVYEEGYSMGTLLTVFGPGEKVGSITIPEET
                     VGIGTVCSVTLNGVLLKAGIPTISRFGGLLEIVDKRPVRFVEIIMYDGTSLDPLEVFI
                     RSGMTDYTGAVTKGTGKIGASFREFPAASRNQVAKLASRLESMGLGGFMRIGHPNLAL
                     LEIPVAEGRFGAVVIGGLNPVAILEENGVKVHSRALAGLVDYDRLIHYDELERRLERF
                     VR"
BASE COUNT          817 a         1416 c         1524 g          886 t
ORIGIN      
        1 tgttgccttt cccgcatctt cgagcgcctg ggcgatgacg aagaccgaga cgtaggaata
       61 gaccgcctcg gcgctcaagg cacccttgca tctcttcccg aacgcatccc tgagccgggc
      121 gatcgacggc ctgtccatgt ccggttccca catggacacg tcgaacaggt attcgcatgc
      181 acgcccggcc ttccggatga acgaggggtc ggaatgcccg ccgccgctgg cgacgacggc
      241 ggcggccttg accttgatgt caccgaatgc cttggcgagg gtctgcgcgt cttcgccgat
      301 cgaggccagg aggatgacgt cgggcttggc gttcgcgacc ttcatggccg ttgccgagag
      361 atcctgtgaa gacgccgggt atgactcggc cagggccacg cggtagccct cggatgccgc
      421 gagcctctgc cactggtctc tcatggacga gccccatggg ccgttctcga acacaaaggc
      481 cagagactgg acaggggcct tcgatcgcct cttcatgtcc gccaggaacc tgaactggtc
      541 cctggatcgc cagctgtcct tgggcgcgag gcggaagacg tacctgagcc ctcgctcggt
      601 gatggagtcc ctcaccgaca ccggcaccac gtaagggatc cccgccgtgt cggcggcctg
      661 ggaggtgggg taggtgaccg cagagttcca tgcgcccgag acgaggtgca ccttcgcctc
      721 cgtgacgagc ctcgcgagct cgctcatgcc cttggtcggg tcgctggcgc tgtccgccca
      781 gacgttctcg agccttgccc cgcccatgga cttgatgccc ccgagcgagt tgatctcctc
      841 ggtggccatc tccaccgcgc acatggcctc ctttccggca acggaggagg ggccggtgag
      901 agggaggatg ttccctatcc tcactgtctt gggcggcgcc tgctgggcgg acagggcggg
      961 atatgccgcg agcaggagca cggccatgat cgcaagacag aactcgagcg gtcttccgcg
     1021 gtgtccctgg cgcaaggatg ctgagcgggg cgagaacacg cgtcccccgt gcccgcgcga
     1081 ggggcgagcc cgaaggatgc ctgggcgcct gccgccacgt gttcgataca cgtctcggcc
     1141 gccctctcct tttcgggcac tgcagtgagg cggaatcaca tcacggttca agccgcaatc
     1201 cacggggcga tcctaccacg gccgggctcg tgtgtcatgc accttgtccc cttggtgtcg
     1261 cctgcatggc gggcatgccg cggaccgctc cggcaagggg caaggccagc cgcacggaag
     1321 acgggctgga accctctcgg gctgagggtg gttgtctctc ggggggcggg tgccgacgag
     1381 gcgggtggat ctcccttgtt tcggcgccat ctgttccggc accatcggcc cggcatgggc
     1441 cttgacccag ggccctcccg gcaggcaggg cgatcggtgc ttcaaccccg gggtgatagg
     1501 gacgagggcg tacaggagag aaggcatcgc agcccgagcg atggaatgcc gatatcgcgt
     1561 gtgaggcggc gccggaggcc ctgcccggca cggggactca cgtggaaggg ggggcgaggt
     1621 ggggcggccc ctgaaacccg gggccgcccg gaaggggtgc gtcaggactt gaggatnnnn
     1681 nnnnnngacg tcggtgatga aggggatgcc ggtcacctcc acggtctcct tgttcgcgga
     1741 gaagaggtct tccctggaga cgccggacaa gctgaacttc ctggcaccgg ccatgagctg
     1801 ctggagacct gcagacagct tgtcgcacag ggtccacatg gcgatggcgc cgtaggggat
     1861 gttttccatc tccttcgccc cgaccttctt ctggacgtcg tagtagccgg cgaagatctc
     1921 gtcggccttg tggccgatat ccgtcacggt cttgggcagc ttttcccagt ggccctgcac
     1981 cttctccctg cgctccggaa agagcgcccc ctcgatgttg gaacccacga agccggggat
     2041 catggccgcc ctgcccatgc agacgagctt cgtgaagggc gctcccagcg cgatggcctt
     2101 gaagatgtgg tcctcccgtg caatgccgcc tgcaaaggcg atgtccacga ctttcttttt
     2161 cttcttcgcg aggatcgagc agtactcgta ggccttggaa tggaggtaga tggagggtac
     2221 gccccacgtt tccatcatgt tccacgggct catgccggtc ccgccgccgg acccgtcaat
     2281 ggtgaggagg tcgagcttgg cctctgtggc gtacttgatg gccatggcca gggcctccat
     2341 gccgtaggat cctgtcttga gcgagatgcg ggagtacccg agcttcctca ggtagttgac
     2401 ctccttcatg aagtcttcct ggacctcttc cggggaaccg aggttggtgt agccgagcct
     2461 gctgtgtctt gcgaacgacc tgatggcgcc gtgcttgaac gcctcctgga cctccgggag
     2521 ggtggggtcc gggtcgacca cgtagccgcg tttcttgagg aagatcgcgt agtcgagaga
     2581 ggtgacctgg atctctccgc cgatgtcctt tgcaccctgg ccccacttga gttctatgat
     2641 gaccttgtcg ccgtacttgt cgatgacgta ctctgccacg ccgtttcggg tgtcctcgac
     2701 gttcatctgg acgatgatgg cgccgtagcc gtcatggtag cgcaggtagg tgtctattct
     2761 gcggtcgagt tccggggcct tggagatctt gccgttcttg atcaccgacg ccttgtccac
     2821 gcccacgacg ttctctccca ccacgatggg tatgcccacg agagaggcgc ccaccgcgaa
     2881 cgactcccag tacttggcgg caacgaaggt ggagccgagg gcgcctgtca tgacgggtat
     2941 cctgctcttg gtcttcacct tggaaccgaa ctcggtctcg atgctgacct tggggaacac
     3001 gcagtcgtcg gccgagtcgg cgagaccctt gggacggttg tatgcgccgt agttgtaccc
     3061 ctggatgcga agggagttgt acgagacgcc gacatgcacg gtgttggagc ttcccgcggt
     3121 cacgatgccg aagtccctcg ggtagaggag cttgcggccg atgaggctgg agagccacgt
     3181 ctcgcacttt cccgtgcagt ccgccctgca gagggtgcac aggcctgact cggcggggtt
     3241 ccccctgttg gcggtgccca gaacatcgtt cgatttctgc cactggatca tgaagaatac
     3301 ctccatacct aagtttgatt ggcaatgcat tgccacaaca atgtgcgata gtggcaattg
     3361 gttgccggat atttgagacc gacagacgtt actatgccca atacagacat ggatgtcaac
     3421 acaaaacacc tttccctgcc gaaatacggt gactgatatc ggcaataatg ccgaaaatgc
     3481 cctccccatg ttgacacctt tcagtgatgc cgctatggtg tcgtgcatga ccgagaagaa
     3541 ggagaagatc cgccacgcca tcctttccat actcagggat gccgagtcgc ccttgagcag
     3601 ttatgccatc agctctcagc tgatcgatcg gggcatagaa atcagcgaac ggaccgtgag
     3661 gctctacctg caggtcatgg acgaggaagg cctcaccatg aagtcgaaca ggaggtgtca
     3721 cgccatcacc gagcagggcg tccaggaact cggctcgtgg cggaccctcg agcgggtggg
     3781 gttcctctcc gcccgcatcg accagatgac ctaccagatg gacttcgacc tcgcgacggg
     3841 tagggggacg gtgatcgtca acatcacgaa cgtcccgccg agggcggccc tcgaacatgc
     3901 ccactccatc aagaaggtct acgaggaagg ctactcgatg gggaccctgc tcaccgtctt
     3961 cggtccgggc gagaaggtcg ggtcgatcac cataccggag gagacggtcg ggatagggac
     4021 cgtgtgctcg gtgacgctca acggcgtgct cctcaaggca ggcattccca ccatatcgag
     4081 gttcgggggt ctgctcgaga ttgtcgacaa gcggccggta cgcttcgtgg agatcatcat
     4141 gtacgacggg accagtctcg accccctcga ggtgttcatc aggagcggga tgacggacta
     4201 cacgggcgcc gtgacaaaag gcacggggaa gataggggcg agcttcaggg aattccctgc
     4261 cgcgagcagg aaccaggtcg ccaagctcgc ctccaggctc gaatccatgg gtctcggcgg
     4321 gttcatgcgc atcggtcacc ccaacctggc gctcctcgag atcccggtcg ccgaagggag
     4381 gttcggtgcc gtggtcatag gcgggctgaa cccggtggcc atcctcgagg agaatggcgt
     4441 gaaggtccac tcgcgggcgc tcgcaggcct ggtcgattac gaccgcctca tccactacga
     4501 tgaactggag agacgcctgg agcgtttcgt acggtgatcc tcgccagagg cggcgtgcat
     4561 gggccaggag accggtgaat cgttggcggc gcgggatcgg ggagcgggag cggcggacct
     4621 gcgcgtggcg gtcataggcg cgttgcgcag gaa
//
LOCUS       DAOSCR010000147         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11689_length_4643_cov_5.227114, whole genome shotgun sequence.
ACCESSION   DAOSCR010000147 DAOSCR010000000
VERSION     DAOSCR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11689_length_4643_cov_5.227114"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..970
                     /locus_tag="PLT69_04940"
                     /pseudo
     CDS             2..970
                     /locus_tag="PLT69_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
     gene            967..1548
                     /locus_tag="PLT69_04945"
     CDS             967..1548
                     /locus_tag="PLT69_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOJ13821.1"
                     /translation="MNTGRHAGFVMLAGMLVAAGCAGATGSHDVTITYRALEDTDRGR
                     IEHVEHGSQVEQDAIERFKEFYRIFSAERIAASMERVYAENAYFEDGLRQVRGRERIK
                     EYFLSTTSAFTECTFDIKDMAYSQGNYYFRWIMHLRLKRDPDHPMEQPGMSHVRFDEQ
                     GRVVFHHDYWETLALFERFPVIGRVIRFIKSRI"
     gene            complement(2021..2635)
                     /locus_tag="PLT69_04950"
     CDS             complement(2021..2635)
                     /locus_tag="PLT69_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOJ13822.1"
                     /translation="MKRMKATKGLFPVIVIIALLPTLAWAVPITTSLNVSNEDLGLAG
                     DFATVVVSYVGEHTVQFSVDANEALLGEDDNFGIQAFGFNASLDLSSAVFDLPAGWDF
                     RYDQNLSMFGLFYIDAYGTGKTRKDPLIFTVSLPGINDENQFFVPNALGYHYAAHIAG
                     FVDHNGQTSAWFSDQPAPVPEPATLMLLGTGLLGLAGFRRKTKR"
     gene            3086..3202
                     /locus_tag="PLT69_04955"
                     /pseudo
     CDS             3086..3202
                     /locus_tag="PLT69_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3220..3372
                     /locus_tag="PLT69_04960"
     CDS             3220..3372
                     /locus_tag="PLT69_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13823.1"
                     /translation="MEIGEEQQGRYFIFEDEVSAEMTGYFLPAMNASAHAGYHLSQQV
                     GSFSNY"
     gene            complement(3377..>4643)
                     /locus_tag="PLT69_04965"
     CDS             complement(3377..>4643)
                     /locus_tag="PLT69_04965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ13824.1"
                     /translation="DIGDKLTFDTIPVYAFEALGIPVPEGEELDMRMLKLKVFSPDKK
                     HSFEADLDGYLAHRRLLGQRLLKYAVDAGAVLLPEHEVIDPILERDYIIGVRCRDRSG
                     KITEYKSKIVVDASGILSVVRDKLPHSVYHNEGIIPQDTVVTYREVRDLKGSSDYIPP
                     HDYPGWYCILQNRGYFWIVPEAKDKTNIGCGIPMFPGHPDPVRVTNEFCSAHADKIGE
                     KVYAKGTGPTPFLPMRACQPELVGNGFMLVGDSGYQVSTNSGFGVPGSVIGGKIAAEV
                     AASAIRKGDVSRKGLWEYNVRYKRGFGSSRPFADAIRIFVQSLSHDDLNTLIKLGLIG
                     SKEFSALWMDRTFSYTMPELIAKFTSGMRNIRLFLKMSAVFLIAKSLERIYKKFPSSI
                     EDFHAWMLKRMLLYALLFKVVGLKRGISY"
BASE COUNT         1119 a         1299 c         1245 g          980 t
ORIGIN      
        1 cgtgaaccgt cgcgccttcc aagacggcaa cttggtcttg tcttccctgt caagggcgcg
       61 caacttccgt ctccatcgca gcaggccgaa caccgtacgc gggagcaagg agaacataga
      121 agcccattac gacctggact cggccttcta cgcgacgttt ctggacagga ccatgacgta
      181 ctcctgcggg atcttcgaca gcccctccga ctccctcgag caggcccagg tgaacaagat
      241 gaggtcgatc atgagcaagg cacggatagg accaaacgac cacgtcctgg agatcgggtg
      301 cggctgggga gggttcgcca tagaggcggt caaggcaagg gggtgctcct ggacgggcat
      361 caccgtatcc agggcccagt acgaccatgc acgcgaatgg gtgaagaggg aaggcctcga
      421 caagagcatc cggatcctct tggaagacta ccggaccatc ggcggctcct ttgacaggat
      481 cgtgtccgtg gaaatgctcg aggcggtggg cagcgagcat ctcggcgagt tcttcgcgtg
      541 ctgtgacagg ctgctcaagc cggatggcgt ggcggtgatc caggtcatca cggtccctga
      601 cagccgctac gagaaacaca taagcagccc gaactggatc cagaaacaca tcttcccggg
      661 cggggagctc ccttagctga ccgccatgtg cgtggccatg acgaggcact cgagactcat
      721 ggtggaatcg gtggaaaaca tcggcccaca ctacgccgag accctgagac actggcgtga
      781 acggttctcg gaggcggccc cgacgctcga ccgcatgggg ttcggcgcgg cattcagaag
      841 gaaatggatt tactacttct ccatctgcga ggcccagttc cgtacgcgct cactgggcga
      901 cctccagatc gttctcacca gggaggggaa cactacgctc tccccacccg cgacaggagg
      961 ggtctcatga acacaggccg ccatgccggg ttcgtcatgc tggcggggat gctcgtggcc
     1021 gcaggctgtg ccggcgccac aggctcgcat gatgtcacca tcacgtacag ggcccttgag
     1081 gatacggaca gagggaggat cgaacacgtg gaacacggtt cccaggtcga acaggatgcc
     1141 atagagaggt tcaaggagtt ctaccgtatc ttctcggcgg aaaggatagc cgcctccatg
     1201 gagcgggtct atgccgagaa tgcctacttc gaggatggct tgaggcaggt caggggcagg
     1261 gagcgtatca aggaatactt cttgagcacc acgagtgcat tcaccgagtg caccttcgac
     1321 atcaaggaca tggcgtacag ccaaggtaac tactacttca ggtggatcat gcacctgcgc
     1381 ctcaagcgtg acccggacca ccccatggag cagcccggca tgtcacacgt gaggttcgac
     1441 gagcagggcc gggtcgtctt ccaccacgac tactgggaga cactcgcgct cttcgagaga
     1501 ttcccggtca tcgggcgcgt gatccgcttc atcaagagca ggatataggc cccctggcct
     1561 ctcgcacgcg cctgcgttca ccccggtgtc agtcatggga ccgtggcagc gtacacacac
     1621 gatcgcctgg cacgtgggtt ctcgcaccca ggacctcggt cccgggtgag ccggtaacag
     1681 cgccgaccgt tgggaacccg gagggcacga cccctcccca cgcaggcacg aggcgccagg
     1741 aaccgcaagg agaagataga agccgcacga cccctcccca cccgggcacg agaacaatcg
     1801 atacgccttg gcactcgcca catacgtagg gatcaaggag cgcccctggc caccgtctcc
     1861 tcgaagcggt aacattcgcc acatgcgtag ggaccaagga agagatcatg cgttgagttc
     1921 atgaaccagt ggtattgccg ttgttccagg tgtagaaatg aatgaggcag ggcctcgatc
     1981 ttgaccggcc ctgccccgta tgtccccagc ggcgaaaccg ttaccgtttc gtcttccttc
     2041 tgaatcccgc aagaccaaga agacccgttc cgagcaacat gagcgtcgcc ggctcgggca
     2101 ccggggcggg ctgatcggag aaccatgcgc tggtttggcc gttatgatca acgaaacctg
     2161 cgatgtgggc ggcatagtgg taaccaagcg cattcgggac gaagaactgg ttctcgtcat
     2221 tgattcccgg caaagaaaca gtgaagatca gtgggtcctt tctcgtcttt cccgtgccgt
     2281 aggcatcgat gtagaacagc ccgaacatgc tgaggttctg gtcgtaccgg aaatcccacc
     2341 ccgcgggcag atcgaagacg gctgacgaca agtcaaggga tgcattgaac ccaaatgcct
     2401 ggatgccgaa gttatcatcc tctcccagga gagcctcatt ggcatcgaca ctgaactgca
     2461 cggtatgttc acccacgtaa gacacgacga ccgtggcgaa atctcctgcc aaccccaggt
     2521 cttcattgga tacgttcagt gacgtggtga tcggaacagc ccaagccagg gtcggaagaa
     2581 gagcgatgat cacgatgacg ggaaacaagc ctttggttgc tttcattctt ttcatggtgt
     2641 cctcccaagg atgcctagca tataatatgc aaaagtcttg cccactgaca taccccttcc
     2701 ttttatatcg ttttatcaat tagttatgaa gttaaacgca agttttcgtc atggagggcc
     2761 ttttgcgatt gtaaaatgta tcgacacaac cttttttcaa agatcacgtt atcgctcgcc
     2821 atatgataat gatttcttct cgttgggatc gtcagaaatc tttacagtgc gatcagtctt
     2881 ggcacgcccc tgccgacagg cacagtctac caacccccat gcccgtaagc gctcacaccg
     2941 aaggatgaag atagtgcagc agaaggccaa tgagttaccc gacaacgttc atgtctcacc
     3001 aaaaccaccg aggatggcag gacatgagcc gtacatattc taacgcagtt ttaagcgtgg
     3061 tgcactctca ttgttgacac tatcaaagat aggtgagcgg atcgggtaca ggaaagcata
     3121 caagccgaga acgctggtta ctcgtttgga acgtatggaa ctcatggtgc ccagggaccg
     3181 tgaggggcgg ttccagacgg agccatcaaa gagctatcaa tggaaatagg cgaggaacag
     3241 caaggccgat acttcatttt tgaagatgaa gtatcggccg aaatgacggg atacttcctt
     3301 cctgccatga atgccagcgc ccatgccgga taccatcttt cacagcaggt cggtagcttt
     3361 tctaattatt gatcaactaa tacgatatcc ccctcttcaa tccgacaacc ttgaacagaa
     3421 gggcatacag caacatccgt ttaagcatcc atgcatgaaa gtcttcgata gaagatggaa
     3481 acttcttata gatcctctct aaactcttag cgatcaggaa cacggctgac atcttcagga
     3541 ataacctaat gtttctcata ccagatgtga atttggcaat gagttcaggc atcgtgtagg
     3601 agaatgtccg gtccatccaa agggcactga attcctttga accaatgagt cctaatttga
     3661 tcagggtatt cagatcatcg tgtgaaaggc tctgtacgaa aatcctgata gcatcagcaa
     3721 agggtcgcga ggaaccgaat ccccttttgt atctcacatt atattcccat agacccttcc
     3781 tgctcacgtc accttttcgt atggccgaag cggcaacttc agccgcgatc ttcccgccta
     3841 tcaccgaacc gggaacgccg aaccccgaat ttgttgagac ctggtatccc gagtcaccga
     3901 caagcatgaa tccgttgcct acaagttcag gttggcaggc cctcatgggc agaaagggcg
     3961 tagggcctgt ccctttggca tataccttct caccgatctt gtctgcatga gcggagcaga
     4021 attcattggt cactctcaca ggatccgggt gcccaggaaa cattggaata ccacaaccta
     4081 tgtttgtctt atcctttgcc tcagggacta tccagaaata accgcgattc tggagaatgc
     4141 agtaccatcc agggtagtca tgaggaggta tgtagtcaga gctaccctta agatccctta
     4201 cctcacgata ggttacgact gtgtcctgtg gtatgattcc ttcattatgg taaaccgaat
     4261 gagggagctt gtcccgcaca acagacaaga tcccgctggc atcaacgacg atctttgact
     4321 tgtactcagt tatcttccca gaacgatccc tgcaccgtac gccaatgata taatctcttt
     4381 ccaagattgg atctatcacc tcgtgttctg ggaggagaac ggcgccggcg tctacagcat
     4441 acttcagaag cctttgcccc aacaaacgcc tgtgcgcgag gtacccgtca agatcagcct
     4501 cgaaagaatg tttcttgtct ggagaaaaca ccttcagttt gagcattctc atatcaagct
     4561 cttccccttc agggactggt atgcccaggg cctcgaatgc gtagaccggg atagtatcga
     4621 aggtgagctt gtctcctatg tct
//
LOCUS       DAOSCR010000148         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11718_length_4636_cov_3.178564, whole genome shotgun sequence.
ACCESSION   DAOSCR010000148 DAOSCR010000000
VERSION     DAOSCR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11718_length_4636_cov_3.178564"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2446)
                     /gene="polA"
                     /locus_tag="PLT69_04970"
     CDS             complement(<1..>2446)
                     /gene="polA"
                     /locus_tag="PLT69_04970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOJ13825.1"
                     /translation="AVYILCRMLLKLIKDKNPSHICFVLDSKGPTVRHERYEAYKATR
                     QKMPEALAVQFPYILEAVEAMGIPIVMKEGLEADDVIATAARRFKASSAVCIVSGDKD
                     LMQLVDDDVTVWDTLKDARYDKDAVRARFGVDPAYIPDLLAVMGDSSDNVPGIRGIGE
                     KGARDLVARFGHLEDIIENALSIDNPRIAKAVSQGADDARLSLELVRLDRDCELDLEL
                     AHIERRPMDLERLNKLFAELEFRGLLDGMSPGLAKTTPSFGGTIEFGCLPLARGEAGF
                     YVLGLRCSALVCAGGATVCTDPRACLDALRSPDALLVMHNAKEAVAEALMRGIEVKAA
                     TFDTMLAEYCCDASSGQSSLEDLAKVYLGEVLPGPKDLLGSGKTAQDPEMIPREELGR
                     FLASHAACLPPLRRAIEERMAALGVDRLFATIETPLSTVLAHMEATGMYVDVSILGAI
                     ARELDAELGLMETRIHELAGKPFNINSPKQLAEILFADLRLPVVKKTKTGPSTDSAVL
                     EALAARHELPAAILNWRTLAKLKNTYVDTLPTMINPATGRLHTRFNQAVTATGRISSS
                     EPNLQNIPIRTGTGRRIREAFRAPEGFVIMSADYSQIELRILAHITKDPLLVESFEQD
                     LDIHAKTASEIFGTPLAQVTENERRQAKTINFGIIYGMGPHKLAGELQIKRDVAKRYI
                     ENYLARYPGVRAYMEDIVREAQEKGFVTTLMGRRRSIPEISSRNFNEREAARRIAINT
                     PIQGSAADIIKLAMVRIHNRMQGMHSRMILQVHDELVFEVALEEIDEVKALVKHEMEH
                     AYPLAVPTHVDIGVGK"
     assembly_gap    2447..2456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2457..3023)
                     /locus_tag="PLT69_04975"
     CDS             complement(<2457..3023)
                     /locus_tag="PLT69_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ13826.1"
                     /translation="MDKGRTIRCPGCGREVKVYENPVPTVDAIVEYEGGIVLVRRKNP
                     PEGWALPGGFVDYGETVEQAVAREVLEETGLGVHGLRLFSVYSAPDRDPRRHTITTVF
                     TAKGDGVLRPGDDAAKACVFSLDDLPELAFDHGRILADYREYLASGRRGDCPPMTDRP
                     TVYLIDGSSYVYRAFYAMRDLSTSTGLPT"
     gene            complement(3024..3668)
                     /locus_tag="PLT69_04980"
     CDS             complement(3024..3668)
                     /locus_tag="PLT69_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13827.1"
                     /translation="MKEKILAHMGSLLSGVVHNLNTPLMWVMGRAQLVQSRIERLASQ
                     DSHSKEEIASVCEKSLKDLGSIIEGAEKIDAILKGLGYKIHMVNEGFTAVELREYLEM
                     EMNFLMADMRFKHDTTKIIELGGSAHYTKVDYNALSSAVTGVIHTMIDRTNKGRTIRV
                     ALNGSVIHLACPELKPDDETRRAIDEATSPLAPMADIFVDDSDGLAVAITVRDR"
     gene            complement(3665..>4636)
                     /locus_tag="PLT69_04985"
     CDS             complement(3665..>4636)
                     /locus_tag="PLT69_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175642.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ13828.1"
                     /translation="ARPLALHGVVVKEDGTSVDIVIGEDEDDPCFTIEDLLPHLAGKA
                     QYGKKLEDAFLGEKLNIVCGTRPDTKAKEEKVKKAILDLVHKKYGIVEQDFTSADIEV
                     VPAGPAREVGFDRSLIGAYGHDDRACAWAAFRAVMDATRVPWTTVVLLVDREETGSDG
                     NTGAKSLFLEETVWAMADRLKRQVTPSRVLFRSRAVSGDVNGALDPDYPEVHEKNNAA
                     RLGYGVCITKYTGSRGKAGSSEASAEYMGWIRSILNRKRIPWQTGELGKVDEGGGGTV
                     AKFLAEYGMDIVDAGPPLLSMHAPFELASKVDVYATYLAYRAFMEEP"
BASE COUNT          658 a         1503 c         1551 g          914 t
ORIGIN      
        1 ctctttccca cgcctatgtc gacgtgcgtg ggcacggcga gggggtaggc gtgctccatc
       61 tcgtgtttga ccagggcctt cacctcgtcg atctcttcca gggccacctc gaagacgagc
      121 tcgtcgtgca cctggaggat catgcggctg tgcatgccct gcatccggtt gtggatcctc
      181 accatggcga gcttgatgat gtccgcggcg ctgccctgga tgggggtgtt gatggcgatg
      241 cggcgggccg cctcgcgctc gttgaagttc ctggacgaga tctccggtat gctccggcgc
      301 ctgcccatca gggtggtcac gaagcccttt tcctgtgcct cgcggacgat gtcctccatg
      361 tacgccctca cgcccgggta gcgggcgagg tagttctcga tgtagcgctt ggccacgtcc
      421 ctcttgatct ggagctcgcc cgccagcttg tgaggcccca tgccgtagat gatgccgaag
      481 ttgatggtct ttgcctggcg tcgctcgttc tcggtcacct gcgcgagcgg ggtgccgaag
      541 atctcggagg cggtcttcgc gtggatgtcg aggtcttgct cgaaggactc gacgaggagg
      601 gggtctttcg tgatgtgggc gaggatcctg agctcgatct gcgagtagtc cgcgctcatg
      661 atcacgaagc cctcgggcgc cctgaacgcc tccctgatgc gcctgccggt gccggtccgg
      721 atggggatgt tctggaggtt cggctccgag gacgatatcc tcccggtggc cgtcaccgcc
      781 tggttgaatc tcgtgtgcag cctgcccgtg gccgggttga tcatggtcgg cagcgtgtcc
      841 acgtaggtgt tcttgagttt cgcgagcgtg cgccagttga ggatggcggc cgggagctcg
      901 tgcctggcgg ccagggcctc gagcaccgcg ctgtcggtgg atggtcccgt cttcgtcttc
      961 ttcaccaccg gcagcctcaa gtccgcaaag aggatctccg ccagctgctt cggcgagttt
     1021 atgttgaacg gcttcccggc gagctcgtgt atcctggtct ccatgagacc cagctcggcg
     1081 tcgagctccc gcgcgatggc gccgaggatg gacacgtcca cgtacatgcc cgtcgcctcc
     1141 atgtgggcga gcacggtgga gaggggcgtc tcgatcgtgg cgaacagccg gtccacgcct
     1201 agggcggcca tgcgttcctc gatggcccgc ctgaggggcg gaaggcacgc ggcgtgcgag
     1261 gcgaggaacc tccccagttc ctccctcggg atcatctccg ggtcctgcgc cgtctttccc
     1321 gaccccagga ggtctttggg gccgggcagg acctcgccga ggtagacctt ggcgaggtcc
     1381 tcgagggagg actgcccgct tgaggcgtcg cagcagtact ccgcgagcat ggtgtcgaag
     1441 gtcgccgcct tcacctcgat gccccgcatg agagcctccg cgacggcctc cttcgcgttg
     1501 tgcatgacga ggagggcatc cggtgacctc agcgcgtcca ggcaggcccg ggggtcggtg
     1561 cacacggtgg cccccccggc acagacgagc gccgagcacc tgaggccgag cacgtagaaa
     1621 ccggcctctc cccgggcgag gggaaggcac ccgaactcga tcgtcccgcc gaaggacggg
     1681 gtggttttcg cgaggccggg agacatcccg tcgagcagcc ccctgaactc gagctcggcg
     1741 aagagcttgt tcagccgctc gaggtccatg ggcctgcgct cgatgtgggc caactcgagg
     1801 tccagctcgc agtcgcggtc cagccggacg agctcgaggc tcagccttgc gtcgtcggcg
     1861 ccttgcgaca ccgccttggc gatccgtggg ttgtcgatgg aaagtgcgtt ctcgatgatg
     1921 tcctcgaggt ggccgaaacg cgccacgagg tcgcgagccc ccttctctcc tatgccccgt
     1981 atgcccggta cgttgtccga gctgtcgccc atgacggcga ggaggtcggg gatgtatgcg
     2041 ggatcgacgc cgaacctcgc cctgaccgcg tccttgtcgt agcgtgcgtc cttgagcgtg
     2101 tcccacacgg tcacgtcgtc gtcgaccagc tgcatgaggt ccttgtcgcc ggagacgatg
     2161 cagactgccg aggaggcctt gaacctcctg gcggcggtcg ctatcacgtc gtccgcctcg
     2221 agcccctcct tcatgacgat ggggatcccc atggcctcca cggcctcgag gatgtagggg
     2281 aactgcacgg cgagcgcctc tggcatcttc tggcgcgtcg ccttgtaggc ctcgtaccgc
     2341 tcgtggcgga cggtggggcc tttcgagtcg agcacgaagc agatgtggga agggttcttg
     2401 tccttgatga gcttgagcag catgcggcac aagatgtaca cggcgtnnnn nnnnnnggtg
     2461 ggcaggcccg tcgacgtgga gaggtccctc atggcgtaga acgccctgta gacgtacgag
     2521 ctcccgtcga tgaggtagac ggtgggcctg tcggtcatgg gagggcagtc cccccttcgt
     2581 ccgctcgcga gatactcccg gtagtccgcg aggatgcgcc cgtgatcgaa ggcgagctcg
     2641 ggcaggtcgt ccagggagaa gacgcaggcc tttgcggcgt cgtcgcccgg cctgagcacg
     2701 ccgtcgccct tggccgtgaa gaccgtggtg atggtgtgcc gcctcgggtc cctgtccggg
     2761 gccgagtaga ccgagaagag tctcaggccg tgaacgccga gccccgtctc ctcgaggacc
     2821 tcccgggcga ccgcctgctc cacggtctcg ccgtagtcca cgaacccgcc gggcagcgcc
     2881 cagccctcag gggggttctt cctccgcacg agcacgatgc cgccttcgta ctcgacgatc
     2941 gcgtccacgg tgggcacggg gttttcgtac accttcactt cccggccgca accggggcac
     3001 cgtatcgtgc ggcccttgtc catctagcgg tccctcacgg ttatggcaac ggcgagcccg
     3061 tccgagtcgt cgacgaagat atcggccatg ggcgccaagg gcgaggtcgc ctcgtcgatg
     3121 gcacgcctgg tctcatcgtc cggcttgagc tcggggcagg cgaggtggat cacgctcccg
     3181 ttcagggcga cccgtatcgt cctgcccttg ttcgtgcggt cgatcatggt gtggatgaca
     3241 cccgtgaccg cggacgagag ggcgttgtag tccaccttcg tgtagtgggc cgaaccgccc
     3301 agctcgatga tcttggtggt gtcgtgcttg aacctcatgt ccgccatgag gaagttcatt
     3361 tccatctcga ggtactcgcg gagctccacc gcggtgaagc cctcgttgac catgtggatc
     3421 ttgtagccca ggcccttgag gatggcgtct atcttctcgg cgccctcgat gatggacccg
     3481 agatccttga gactcttctc gcacacggag gcgatctctt ccttggagtg gctgtcctgc
     3541 gaggcgaggc gctcgatgcg ggactggacg agctgggccc gtcccatgac ccacatcagg
     3601 ggggtgttca ggttgtgcac caccccgctc aagagagagc ccatgtgcgc gaggatcttt
     3661 tccttcatgg ttcctccatg aacgcccggt aggcgaggta tgtcgcgtag acgtccacct
     3721 tggaggcgag ctcgaagggc gcgtgcatgg agagcagggg aggcccggcg tccacgatgt
     3781 ccatgccgta ttccgcgagg aacttcgcca cggtgccccc gccgccctcg tccaccttgc
     3841 cgagctcgcc ggtctgccag gggatgcgct tccggttcag gatggacctg atccagccca
     3901 tgtactcggc gctcgcctcg ctgctgcccg ccttgcccct gctcccggtg tacttggtga
     3961 tgcacacgcc gtagccgagg cgtgccgcgt tgttcttctc gtgcacctcg ggatagtcgg
     4021 ggtcgagcgc gccgttgacg tcgccggaga cggccctgct cctgaaaagc acgcgggagg
     4081 gggtgacctg ccgcttgagc ctgtccgcca tcgcccagac ggtctcctcg aggaagaggc
     4141 tcttcgcgcc ggtgttgccg tcgctgcccg tctcctccct gtccacgagg agcaccaccg
     4201 tggtccacgg cacgcgcgtg gcgtccatga cggcgcggaa ggccgcccag gcgcaggccc
     4261 ggtcgtcgtg gccgtatgcg cctatgagcg agcggtcgaa ccccacctcc ctggccgggc
     4321 ccgcagggac gacctcgatg tccgcggacg tgaagtcctg ctccacgatg ccgtatttct
     4381 tgtggaccag gtcgaggatc gccttcttga ccttttcctc cttcgccttg gtgtccggcc
     4441 tggtgccgca gacgatgttg agcttctctc ccaggaaggc gtcctcgagc ttctttccgt
     4501 actgcgcctt tcccgcgagg tggggcagga ggtcctcgat ggtgaagcag gggtcgtcct
     4561 cgtcctcgcc tatcacgatg tcgacggacg tgccgtcctc cttcacgacg acgccgtgca
     4621 gggccagcgg cctggc
//
LOCUS       DAOSCR010000149         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11836_length_4602_cov_3.576644, whole genome shotgun sequence.
ACCESSION   DAOSCR010000149 DAOSCR010000000
VERSION     DAOSCR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11836_length_4602_cov_3.576644"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PLT69_04990"
     CDS             complement(<1..1199)
                     /locus_tag="PLT69_04990"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOJ13829.1"
                     /translation="MKKNGRKVVLAYSGGLDTSVILKWLVDEFGYEVICFVADLGQAE
                     ELEGIEEKAKKTGARKVYVEDLREEFVRDYVFPAYRADVAYEGTYLLGTSLARPLIAK
                     RLVEIARKEKAFAVAHGATGKGNDQVRFELGVWALEPDLKVIAPWKMDEWTLRSREKM
                     IDYAKKHGIEVPVTKKKPYSMDRNLLHISFEGGVLEDLWNEPAEDMFVLTRSPEKAPD
                     RPTTLVVSFKDGDPVAVDGKKMSPARLLAHLNAVGGANGVGRVDIVENRYVGMKSRGV
                     YETPGGTILKVAHRGIEQITMDREVMHLRDELMPRYATLVYNGYWFSPEREVLQKLID
                     ETQKGVTGDVRVKLYKGSCMVTGRRAKVSLYDPEIATFGEDEVYDQKDAGGFIRINAL
                     RLKVRAKV"
     gene            complement(1211..2113)
                     /gene="argF"
                     /locus_tag="PLT69_04995"
     CDS             complement(1211..2113)
                     /gene="argF"
                     /locus_tag="PLT69_04995"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOJ13830.1"
                     /translation="MEHRDFLTIRDLQAEEIGEIFDRARRFRDAIPAGRLSGRSVVLV
                     FEKASTRTRVSFEVGVARMGGHPVVLGVEGSQIARGEPLSDTARILSGYCDAIVMRTY
                     GHDRIEEMARWASVPVVNALTDRFHPCQVLSDCFTLEGRFGGIRGRKVAWIGDGNNMA
                     NSWINAAAVLGFELVLACPEGYDPDAYVVSSAVSDGAKVRIVRDPMEAAEGAIAVNTD
                     VWASMGREDEAQTRARVFEPFRVDERVMSRADPRAVFMHCLPAHRGEEVTDEVLEGGR
                     SIVWEQALNRLYVQEAILDFLLNG"
     gene            complement(2113..3276)
                     /locus_tag="PLT69_05000"
     CDS             complement(2113..3276)
                     /locus_tag="PLT69_05000"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOJ13831.1"
                     /translation="MEHVMGTYARQPVRFVRGQGCRLYDESGRGYLDLVSGIAVCNLG
                     HAHPEVVAAVKDQAEKLFHCSNLYEIPLQETVAKMLADNAFEAKVFFCNSGAEANEGA
                     IKLVRRAARSRGKPKPRIITARESFHGRTLATLAATGQEKYRQGFDPIPEGFSTVPYG
                     DIEALEASIDGDVGGVMLEPIQGEGGIRVPPGGYFEKVRRICDAAGILLVLDEVQTGM
                     GRTGRFFAHQHEGITPDVMTLAKALGNGFPVGAVLARPELADVMTPGSHASTFGGNPL
                     AMAAAHATLSVMLREDIPARAHELGEYLMERLNGLAPSHPSIVEVRGRGLMTGVEFSC
                     DVSFLPAEGLRHGVLLNVIQGRVLRLTPPLVVSREEIDKGVEAIDAILGEKGL"
     gene            complement(3282..4166)
                     /gene="argB"
                     /locus_tag="PLT69_05005"
     CDS             complement(3282..4166)
                     /gene="argB"
                     /locus_tag="PLT69_05005"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ13832.1"
                     /translation="MEHAIQRAKVLVEALPYIRDLWGRRVVVKYGGHAMVDPELKRLF
                     ARQIVLFQHIGIQVVVVHGGGPQITSMLDMMGIETRFVEGKRVTDAKTMDVVEMVLAG
                     GINKEIVHLINAEGGRAVGLSGKDGRLIRARKLYSSRTHPDGTVTRDLDIGHVGEVEE
                     IDPSVIDAVEKAGYVAVIAPSGVGSDGLTYNINADTVASGIAARLKVEKLVILTDEAG
                     VLDADGRLISSLTTSEIPALVKRGVVTGGMIPKLECCVEALEAGVVKAHIVDGRVPYS
                     IMLELFTDSGIGTQVVKG"
     gene            complement(4392..>4602)
                     /locus_tag="PLT69_05010"
     CDS             complement(4392..>4602)
                     /locus_tag="PLT69_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13833.1"
                     /translation="DAFLGGRPRYVPGVFNKVLYLSLVRITPRRVMDLLLRVLTKRLS
                     EIFPKNAAALLSGGEGAKGQGTRAK"
BASE COUNT          736 a         1658 c         1340 g          868 t
ORIGIN      
        1 accttcgccc tcaccttgag cctgagcgcg ttgatcctga tgaaccctcc cgcgtccttc
       61 tggtcgtaca cctcgtcctc gccgaacgtg gcgatctccg ggtcgtagag tgagaccttc
      121 gccctcctcc cggtcaccat gcaggagccc ttgtagagct tgacgcggac gtctcccgtg
      181 acccccttct gggtctcgtc gatgagcttc tggagcacct ccctctccgg cgagaaccag
      241 tacccattgt acaccagggt tgcgtagcgg ggcatgagct cgtcgcgcag gtgcatgacc
      301 tccctgtcca tggtgatctg ctcgatcccg cggtgcgcga ccttgaggat ggtcccgccg
      361 ggtgtctcgt acacgcccct ggacttcatg cccacgtaac ggttctccac gatgtccacg
      421 cggcccacgc cgttggcccc gcccacggcg ttgagatgcg ccaggagcct cgctgggctc
      481 atcttcttcc cgtcgacggc caccgggtct ccgtccttga aggagacgac gagggtggtc
      541 ggcctgtcgg gagccttctc gggcgagcgg gtcagcacga acatgtcctc ggcgggctcg
      601 ttccagaggt cctcgagcac ccccccctcg aagcttatgt gcagcaggtt gcggtccatg
      661 ctgtaaggct tcttcttcgt gacagggacc tctatgccgt gcttcttggc gtaatcgatc
      721 atcttctccc tggacctgag cgtccactcg tccatcttcc acggcgcaat gaccttgaga
      781 tccggctcga gcgcccagac gccgagctcg aaccgcacct ggtcgttgcc cttgcccgtc
      841 gccccgtgtg caaccgcgaa ggccttttcc ttcctggcga tctccacgag cctcttggct
      901 atgagaggcc tggccaggga cgtcccgagg aggtaggtcc cctcgtacgc cacgtccgcc
      961 ctgtaggcgg gaaacacgta atcccgcacg aactcctctc tcaggtcctc gacgtagacc
     1021 ttcctggcgc cggtcttctt cgccttctcc tcgatgccct cgagctcctc ggcctggccg
     1081 aggtcggcca cgaagcagat cacctcgtag ccgaactcgt cgacaagcca cttgaggatg
     1141 accgacgtgt ccagcccacc ggagtatgcc agcaccacct tcctgccgtt cttcttcatg
     1201 tctcgccccc tcagccgttg agtaagaaat cgagtatcgc ttcctggaca tacagcctgt
     1261 tcagggcctg ctcccacacg atggaacgcc cgccctcgag aacctcatcc gtgacctcct
     1321 cgcccctgtg cgccggcaga cagtgcatga acacggcccg tggatcggcc cgggacatga
     1381 cccgttcgtc cacgcggaag ggctcgaaca cccgtgccct tgtttgcgcc tcgtcctccc
     1441 tgcccatgct cgcccagacg tccgtgttca ccgcgatcgc cccttcggcc gcctccatgg
     1501 ggtctctcac gatacgcacc ttcgcgccgt ccgagacggc cgaactcacc acgtacgcgt
     1561 ccggatcgta cccctcggga caggcgagca caagctcgaa tcccagcacg gcggcggcgt
     1621 ttatccacga gttcgccatg ttgttgccgt cacctatcca ggccaccttc ctgcccctga
     1681 taccgccgaa acgcccctcg agcgtgaagc agtccgacag gacctggcag gggtggaacc
     1741 ggtcggtgag cgcattgacc acgggcaccg aggcccacct agccatttcc tcgatgcggt
     1801 cgtgcccgta ggtgcgcatg acgatggcgt cgcagtaacc ggagaggatc ctcgccgtgt
     1861 cgctgagggg ctcccccctg gcgatctgcg acccctcgac cccgagcacc acggggtgtc
     1921 cgcccatcct cgccacaccg acctcgaagg acaccctggt ccgtgtcgac gccttttcga
     1981 agacgagcac caccgacctg ccggagagtc tccccgcagg gatcgcgtcc ctgaacctgc
     2041 gcgcccggtc gaaaatctcc ccgatctcct ccgcctggag gtcccttatg gtgagaaaat
     2101 ccctgtgttc catcacaagc ccttctctcc gaggatggcg tctatggcct cgacgccctt
     2161 gtctatctct tcccggctca ccaccagcgg cggggtcagc ctgagcaccc tgccttggat
     2221 gacgttgagg agaaccccgt gcctgagccc ctcggccggc aggaacgaaa cgtcgcacga
     2281 aaactccacc ccggtcatga gccccctgcc cctgacctct acgatcgacg ggtgcgacgg
     2341 cgcgaggccg ttcaggcgct ccatgagata ctccccgagc tcatgagccc tcgcggggat
     2401 gtcttctctg agcatgaccg aaagcgtcgc gtgcgcggcc gccatggcca gcgggttccc
     2461 gccgaaggtc gatgcatgcg aaccgggcgt catcacgtct gcgagctcgg gcctcgcgag
     2521 gacggccccg acggggaacc cgttcccgag agcctttgcg agggtcatga cgtcgggcgt
     2581 gatcccctcg tgctgatgcg cgaagaacct gcccgtgcgg cccatgcccg tctgcacctc
     2641 gtcgagcacg aggagtatcc ccgcggcgtc gcatatcctg cggaccttct cgaaataccc
     2701 ccccggcggc accctgatgc cgccctctcc ctggatgggc tcgagcatga cgccgcccac
     2761 atcgccgtcg atggaggcct ccagcgcctc gatgtccccg taggggacgg tcgagaaccc
     2821 ttcggggatc gggtcgaacc cttgccggta cttttcctgt ccagtggctg cgagcgtcgc
     2881 caacgtcctg ccgtggaagg attcccgcgc ggtgatgatg cggggcttgg gcttgcccct
     2941 ggagcgcgcc gccctcctca cgagcttgat cgccccctcg ttggcctcgg ctcccgagtt
     3001 gcagaagaag accttcgcct cgaaggcgtt gtcggccaac atcttcgcga ccgtctcctg
     3061 aagggggatc tcgtagaggt tcgagcagtg gaagagcttc tccgcctggt ccttgaccgc
     3121 cgcgaccact tcagggtgcg catgtccgag gttgcacacc gcgatgcccg agacgaggtc
     3181 gaggtaccct ctgccgcttt cgtcgtagag cctgcagccc tggcccctca cgaacctgac
     3241 gggctgcctg gcgtaggtcc ccatcacgtg ttccacggtt ctcacccctt cacgacctgc
     3301 gtgcctatgc ccgagtcggt gaagagctcg agcatgatgg agtacggcac gcggccgtcc
     3361 acgatgtgcg ccttgacgac gcccgcctcc agcgcctcga cgcagcactc cagcttgggg
     3421 atcatccctc ccgtgaccac gccgcgtttg accagcgccg ggatctcgga cgtggtgaga
     3481 ctcgagatga gccgcccgtc cgcatccagc accccggcct cgtcggtgag gatgacgagc
     3541 ttctccacct tcaggcgcgc cgcgatgccc gaggcgacgg tgtccgcgtt gatgttgtag
     3601 gtgagcccgt cggaacccac cccggagggc gcgatcaccg cgacgtaccc ggccttctcg
     3661 accgcgtcga taaccgacgg gtctatctcc tcgacctctc ccacgtggcc gatgtccagg
     3721 tccctcgtca ccgtcccgtc ggggtgggtg cggctggagt agagcttcct cgccctgatg
     3781 agccgcccgt ccttgcccga cagccccacg gccctccccc cctcggcgtt tatgaggtgg
     3841 acgatctcct tgttgatccc accggcgagc accatctcca ccacgtccat ggtcttggcg
     3901 tcggtgacgc gcttgccctc gacgaaccgc gtctcgatgc ccatcatgtc gagcatggag
     3961 gtgatctggg gcccgcctcc gtggacgacg accacctgga tgccgatgtg ctggaacagg
     4021 acgatctggc gggcgaagag cctcttgagc tccgggtcca ccatggcgtg gccgccgtac
     4081 ttgaccacga ccctcctgcc ccacaaatcc ctgatgtaag gcagcgcttc caccaggacc
     4141 ttcgccctct gaatggcatg ttccatgggt tcgcacccct tgcggcacag ctctcggtac
     4201 aacgtccgcc gcgggcggct ttaccgtcgc ccacgggaaa gcagtttttt atccaaatcc
     4261 tcgagaaaag aaaaggggaa aatacggcct ctggcgcatc tcaccagggg ccgccggggg
     4321 acctccccga ggggaccgag acatgcccga tgttcatcga cgacgcgaca aggggctcgc
     4381 gtgcacccct ttcacttcgc ccgtgtgccc tgcccctttg ccccttcacc acctgacaac
     4441 aacgcggcgg cattcttggg gaagatctcg gagagcctct tcgtgaggac gcgcaggagg
     4501 aggtccatga cgcgcctggg cgtgatgcgg acgagcgaga ggtagaggac cttgttgaac
     4561 acccccggga cgtagcgcgg acgtccgccg agaaaggcgt cc
//
LOCUS       DAOSCR010000150         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11857_length_4596_cov_3.018498, whole genome shotgun sequence.
ACCESSION   DAOSCR010000150 DAOSCR010000000
VERSION     DAOSCR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11857_length_4596_cov_3.018498"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLT69_05015"
     CDS             <1..611
                     /locus_tag="PLT69_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ13834.1"
                     /translation="PGIVRALCICKDRPEPMRAAFSSFNNRIAPVFDVARQLFIVESD
                     AGRIVHQSQENVPAEDVCAKVRRLMELGVETLVCGAISRSTQGLVSSCGIEVIPFVAG
                     DLQEVIQAWLQGRIHDHDYAMPGCCAQGRRNPLRGMGARQGRCDRWEAGERDSCGTCV
                     CPQCSHSEPHERGVPCSQKRCPVCGSALVRGDPGKEQRRTRT"
     gene            635..946
                     /locus_tag="PLT69_05020"
     CDS             635..946
                     /locus_tag="PLT69_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13835.1"
                     /translation="MFRWGKRAFTRTLGRNLPGVPMPPGRLFRRAGVLGPIVAWLAGM
                     AIHDLRQPQSRIRAFARRMLERLSKPRRVTAVVVGPEAGPGHGGLIEQAGKNGKRGKE
                     G"
     gene            972..1415
                     /locus_tag="PLT69_05025"
     CDS             972..1415
                     /locus_tag="PLT69_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOJ13836.1"
                     /translation="MKKIGIIICDRYRSCGGGKCFRALKERCGGFACYPPGEPVEVVG
                     FSSCGGCPGGNVEYVPAEMKENGAEVIHLATGFVVGYPPCPYTKDHKEFIENHYGLKV
                     VVGTHPIPMKYLRAHERLGFWEREDMKELAGALMDEPPDVMTAYD"
     gene            1457..1819
                     /locus_tag="PLT69_05030"
     CDS             1457..1819
                     /locus_tag="PLT69_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ13837.1"
                     /translation="MKIAFTTSGHSLDAPLDARFGRAPRILVYDLDTGTFEVVDNTQN
                     LNAPQGAGIQAAQNVARTGATHLVTGHCGPKAFKVLTAAGIAVYTTDAATVAQALELF
                     RSGGLTRATSADVEGHWV"
     gene            1816..2346
                     /gene="tsaA"
                     /locus_tag="PLT69_05035"
     CDS             1816..2346
                     /gene="tsaA"
                     /locus_tag="PLT69_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOJ13838.1"
                     /translation="MRKASVRTGHITYTPIGVIRSTHTDPEKTPIQGVYAKGCLGRAE
                     VLPEYEEGLKDLEGFSHLILIYHFHRADSVRLTVRPFLQDADHGVFSTRAPCRPNPIG
                     LSIVELVRRQGRTLHLDGVDILDNTPLIDIKPYVGRFDRIEKTRDGWQHEVDEPTARR
                     KGRRGYDPKNDPEAAP"
     gene            2343..3215
                     /locus_tag="PLT69_05040"
     CDS             2343..3215
                     /locus_tag="PLT69_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13839.1"
                     /translation="MIISVASGKGGTGKTTVSANLARVWPSPVQLIDCDVEEPNCHLF
                     IKGELMARDVVSMRVPQVDAALCTGCGACSDICEFNAIAALRDDALVFEELCHGCGGC
                     TLVCPTRAITEKDRRIGGIEVIQAGHICLVQGRIDVGVAMAPPVIRAVKARLDPGKTA
                     ILDAPPGTSCPVIATVKDTDLVILVTEPTPFGLNDLRLAVDMVRALGLPFGVVVNRVG
                     AGDDRVHVFCRDEGVPILMEIPDDRRIAEAYSRGELMVDALDGYRGLFEGLVHTVQGL
                     RTRTRREDHADLRV"
     gene            3196..3444
                     /locus_tag="PLT69_05045"
     CDS             3196..3444
                     /locus_tag="PLT69_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ13840.1"
                     /translation="MPTYEYECNDCRVRFEREQSMSDAPLVTCPECGGRVRRLVTGGT
                     GFINKTGSHGQKGPCSLETTGRTCCGRDQRCGAPRCEE"
     gene            3453..4355
                     /locus_tag="PLT69_05050"
     CDS             3453..4355
                     /locus_tag="PLT69_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13841.1"
                     /translation="MERDTQDIRELVVVSGKGGTGKTSLAASLAVLAQNAVIADCDVD
                     ASDLHLLLDPQVLERHEFVSGHEAVIRQQDCTGCGACIDLCRYAAIAARRDDAGRKVC
                     SVDPVSCEGCGVCVRFCPERAIDFPERSCGEWMISRSRSGPMVHARLGIAAENSGKLV
                     SLVRREARRIAGEEGRSLIIVDGPPGIGCPVIASLGGASQVLVVTEPTVSGEHDLERV
                     LALARHFGVPAGVCVNKWDINPEMTRRIEDMARTAGAQVAGRIRYDAGVTSAQVAART
                     VVETECPAALDIRELWERITHSGG"
     gene            4363..>4596
                     /locus_tag="PLT69_05055"
     CDS             4363..>4596
                     /locus_tag="PLT69_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOJ13842.1"
                     /translation="MHEELDLSPVAREHASRPLNEGPPPRFNAYGRVTGPCGDTMEIW
                     LLVQDGIVERASFTTTGCGPSRACGSMATSLASG"
BASE COUNT          810 a         1459 c         1642 g          685 t
ORIGIN      
        1 cccctggcat agtccgtgct ctgtgcatct gcaaggacag gcctgaaccc atgagagccg
       61 cattcagctc ctttaacaac cggatcgccc cggtcttcga cgtggcaagg cagctcttca
      121 tcgtggagag cgacgcgggc cgcatcgtgc accagtccca ggagaacgtg cccgccgaag
      181 acgtatgcgc caaggtcagg cgcctgatgg agctcggggt cgagacgctt gtctgcggtg
      241 ccatatcgcg ctccacgcag gggctggtct cctcctgcgg catagaggtg atcccgttcg
      301 tggccgggga cctgcaagag gtgatccagg catggctcca aggcaggatc catgaccatg
      361 actatgccat gcccgggtgc tgtgcccagg ggaggcgaaa ccccctcagg ggcatggggg
      421 cgcggcaggg ccggtgcgac aggtgggagg caggggaacg ggactcctgc gggacctgcg
      481 tctgcccgca gtgctcccac agcgaaccgc acgaacgggg cgtcccctgt tcgcagaagc
      541 gctgccccgt gtgcgggagt gcgctggtca ggggcgaccc cggaaaggaa cagaggagga
      601 ccaggacgtg aatactcgcc agggaggacg gtggatgttc aggtggggga aacgtgcctt
      661 cacaaggacc cttggcagga acctgcccgg ggttcccatg ccgccgggcc gcctcttccg
      721 gcgggcgggg gtcctcgggc ctatcgtcgc ctggctcgca ggcatggcca tacacgacct
      781 gaggcagccg cagagccgca tcagggcctt tgcgcggcgc atgctggaaa ggctcagtaa
      841 accgcggcgc gtcacggccg tcgtggtggg gccggaagcc ggacccgggc acgggggtct
      901 gatcgaacag gcaggtaaga atgggaagag ggggaaagag ggatgagccc gagggtcccc
      961 gggggtagtg catgaagaaa atcggcatca tcatctgtga ccgctaccgt tcgtgcgggg
     1021 gcggcaagtg tttcagggcg cttaaggagc gctgcggcgg ctttgcgtgc taccctccag
     1081 gcgagcccgt cgaggttgtg gggttttcca gctgcggcgg ctgcccgggg ggcaacgtgg
     1141 aatatgtccc tgcggagatg aaggaaaacg gcgccgaggt catccacttg gcgaccgggt
     1201 tcgtcgtggg gtacccgccg tgcccgtaca ccaaggacca caaggaattc atcgagaacc
     1261 actacggcct gaaggtggtg gtgggaaccc accccatccc tatgaagtac ctcagggccc
     1321 atgagcggct cggcttctgg gagcgcgagg acatgaagga gctcgccggc gccctcatgg
     1381 atgaaccccc ggatgtcatg acggcctatg actgagcatc ggccgcccag gaaggaaagg
     1441 ggaaaggaga agaaccatga agatcgcgtt caccacctcg ggccacagcc tggacgcgcc
     1501 cctggatgcc aggttcggca gggcgcccag gattctcgtc tatgatctcg ataccgggac
     1561 cttcgaggtc gtggacaaca cccagaacct gaacgccccc cagggcgcgg ggatccaggc
     1621 ggcccagaac gtggcccgga ccggggcgac gcacctggtg accgggcact gcgggccgaa
     1681 ggcattcaag gtgctcaccg ccgcagggat cgccgtgtac accacggatg cggccaccgt
     1741 ggcccaggcc ctggagctct tccgctcggg cggcctcacc agggccacct cggcggacgt
     1801 ggagggtcac tgggtatgag gaaggcgtct gtccgcacgg ggcacatcac ctatacgccc
     1861 atcggcgtca tccggagcac acatacggac ccggaaaaga cgcccatcca gggcgtttat
     1921 gcgaagggct gcctcggcag ggccgaggtg ctgcccgagt acgaagaggg gctgaaggac
     1981 ctggagggct tctcccacct catcctgatc taccacttcc accgggcgga ctcggtacgc
     2041 ctcacggtgc ggccctttct ccaggacgcc gatcacgggg tcttctccac ccgggcgccg
     2101 tgcaggccca accctatcgg cctgagcatc gtggagctcg tgcgcagaca aggccgcacc
     2161 ctgcacctcg acggggtgga catcctcgac aacacgccgc tcatcgacat aaagccttac
     2221 gtgggcaggt tcgaccgcat cgagaagacg agggacgggt ggcagcacga ggtggacgaa
     2281 cccacggcac ggcgcaaggg gaggcgggga tacgacccga agaacgaccc ggaggcggcg
     2341 ccgtgatcat ctccgtcgca tccggcaagg gcggcacggg caagaccacg gtgtccgcga
     2401 acctggcacg ggtctggccc tcgccggtgc agctcatcga ctgcgacgtg gaggagccca
     2461 actgccacct gttcatcaag ggcgagctca tggcccgaga cgtggtctcc atgcgcgttc
     2521 cccaggtgga tgcggcgctc tgcaccggct gcggggcctg cagcgacatc tgtgagttca
     2581 atgccattgc cgcgctcaga gacgatgcac tggtgttcga ggagctgtgc catgggtgcg
     2641 gcggctgcac cctggtctgt cccaccaggg ccatcaccga gaaggaccgc cgcatcggcg
     2701 ggatcgaggt catccaggcc gggcacatct gcctggtgca gggcaggata gacgtgggcg
     2761 tggccatggc gccgcccgtg atccgggccg tgaaggcccg cctcgatccc gggaagacgg
     2821 ccatcctgga cgcacccccg ggcacctcgt gcccggtcat cgccacggtc aaggacacgg
     2881 acctggtcat cctggtcacc gagcccacgc ccttcgggct caacgacctg aggctggctg
     2941 tcgacatggt gcgcgctctc ggcctcccct tcggggtggt ggtgaaccgg gtgggtgccg
     3001 gcgacgaccg ggtccacgtc ttctgcaggg acgagggcgt gcccatcctc atggagatcc
     3061 cggacgaccg ccgcatcgca gaggcctatt cccgggggga gctcatggtg gatgcgctgg
     3121 acgggtaccg ggggctcttt gaagggctcg tgcacaccgt gcagggtttg agaaccagga
     3181 caaggagaga ggaccatgcc gacctacgag tatgagtgca acgactgccg cgtgcgcttc
     3241 gagcgcgagc agtccatgag cgacgcgccg ctggtgacgt gtcctgaatg cggcggccgg
     3301 gtgcgccgcc tggtgacagg cggcaccggc ttcatcaaca agacaggcag ccacgggcaa
     3361 aaaggcccct gctccctgga gaccacgggc aggacctgct gcggcaggga ccagcgttgc
     3421 ggcgctcccc ggtgcgagga gtgagccccg ccgtggagag ggacacccaa gacatccgtg
     3481 agctggtggt agtaagcggc aagggcggca cgggcaagac cagcctggcc gcatcactgg
     3541 ccgtgctggc gcaaaacgcg gtcatagcgg attgcgacgt ggacgcctcc gacctgcacc
     3601 tgctcctcga cccgcaggtg ctcgagcggc acgagttcgt gagcgggcac gaggcggtga
     3661 tccgccagca ggactgcacc gggtgcggcg cctgcatcga cctgtgccgg tacgcggcga
     3721 tcgccgcccg gcgggatgat gcgggcagga aggtctgttc cgtggacccg gtgtcctgcg
     3781 agggttgcgg ggtgtgcgtg cgcttctgcc cggagcgagc catcgacttc ccggagcgct
     3841 cctgcgggga gtggatgatc tcgcggagtc ggagcgggcc catggtgcac gcacgcctcg
     3901 gcatcgcggc cgagaactcg ggcaagctgg tctccctggt tcgcagggag gcccgccgca
     3961 tcgccgggga agaggggcgt tccctgatca tcgtggacgg gccgccgggc atcggctgcc
     4021 cggtcatcgc gtccttgggg ggcgcttcgc aggtgctcgt cgtcaccgag cccacggtat
     4081 cgggggagca cgacctggag agggtcctcg ccctggccag gcacttcggt gtcccggcgg
     4141 gtgtgtgcgt gaacaagtgg gacatcaacc ccgagatgac ccggcgcatc gaggacatgg
     4201 cacgcactgc gggtgcgcag gtcgcgggcc gcatacgcta cgacgcgggc gtcacctccg
     4261 cccaggtggc ggcacggact gtggtggaga ccgagtgccc ggcggcactc gacatcaggg
     4321 aactctggga gcgcataacc cattcgggag gatgacaggg agatgcacga agaactggac
     4381 ctttcgcccg tggcgcgcga gcacgcgtcc aggccactga acgaggggcc gccgcccagg
     4441 ttcaacgcct acgggcgcgt caccggcccc tgcggggata ccatggagat ctggcttctc
     4501 gttcaagacg ggatcgtgga acgggcctcc ttcaccacca cggggtgcgg gccttcccgg
     4561 gcctgcggga gcatggccac ctccctggcc tcgggc
//
LOCUS       DAOSCR010000151         4578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11907_length_4578_cov_3.023878, whole genome shotgun sequence.
ACCESSION   DAOSCR010000151 DAOSCR010000000
VERSION     DAOSCR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11907_length_4578_cov_3.023878"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLT69_05060"
     CDS             complement(<1..403)
                     /locus_tag="PLT69_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOJ13843.1"
                     /translation="MKTDTVLLEHGSGGMLTNRLIREMFVGSFSNEHLDRMDDAAVVR
                     VGEGRLCFTTDSFVVDPIFFPGGDIGSLAVHGTVNDIAACAGRPLFLSAGFIIEEGFA
                     MEELARIVDSMARASREAGVKVVTGDTKVVPK"
     gene            complement(416..2698)
                     /gene="hypF"
                     /locus_tag="PLT69_05065"
     CDS             complement(416..2698)
                     /gene="hypF"
                     /locus_tag="PLT69_05065"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOJ13844.1"
                     /translation="MKSRIRLRFSGIVQGVGFRPFVYRTALSHGMAGFVRNDADGVTV
                     EAEGAEGLVDAFVSSLLEKTPPLAEIHGVLRERVPPLGEKAFRIVASDATGRAEALIS
                     PDVATCDACLAELFDPSNRRYRYPFINCTDCGPRLTIVEDVPYDRERTSMSVFPMCEQ
                     CRKEFEDPGDRRFHAQPNACPVCCPRLTLVDREGRGIRTDDPLAWAANLIREGGILAV
                     KGLGGFHLCVNALYDKAVARLRERKCREEKALAVMARDLVTAERLVHLGEEERRLLRS
                     PSRPIVICREREVSPVSRLVAPSMGTLGVMLPYTPLHHLILEKDPPVLVMTSANLTDE
                     PICIDNEEAVERLKDVADAFVLHNRNIVVRCDDSVAIATERGPTVVRRSRGYAPRPVL
                     LGARMPEVLALGAHLKSTVCVVRDDLAFLSPHVGDLETPLARDFFLETVETMQRIARC
                     RPGVLACDMHPDYFSTRYALGKASSGVIRVQHHHAHIVSCMAENRLKGPVIGLAMDGT
                     GYGEDSTIWGGEFLIATETAFERAGHLKCVALPGGEAAVRQPWRTGASLLRGCFGEEW
                     QEIAGRLGLVPAGIDPAQIEALLESGLNCPVTSSLGRLFDGVSALLGLCRRSSYEGQA
                     AIVLEACADRTYAEVLPFVVDDTRKPLVVDLSAAVSKIVERRLSGEDASRLASAFHAT
                     VVEALAGTAVRLRGMTGIGTVVLSGGCFQNRLLLQGCASRLEGEGFSVATHARVPPND
                     GGISLGQAVVAASRLAYGNV"
     gene            complement(2704..3372)
                     /gene="hypB"
                     /locus_tag="PLT69_05070"
     CDS             complement(2704..3372)
                     /gene="hypB"
                     /locus_tag="PLT69_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOJ13845.1"
                     /translation="MPVINVVRQVLDSNDRIAGGNRALFDEKGIYVMNLMSSPGAGKT
                     TLVEKTIDALKGRYRIAVIEGDIQSTHDAERVARHGVPVVQINTGGGCHIDANMVREA
                     LPGLPLDEVDVLINENVGNLVCPAEFTIGENDKVMILSTPEGADKPSKYPLMFHESSV
                     MIINKIDLLPYVEFDIVKAKADALACNPELVIIEVSCRTGQGLDAWVEWLTTRVETFR
                     KGRA"
     gene            complement(3392..4480)
                     /gene="hypD"
                     /locus_tag="PLT69_05075"
     CDS             complement(3392..4480)
                     /gene="hypD"
                     /locus_tag="PLT69_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOJ13846.1"
                     /translation="MKLIDEYRDNELLVRLRERIHRQARGIGRGVTLMEVCGTHTYAI
                     GRYGIRRILPENVRLISGPGCPVCVTSASDVGRALDMAGKKGVLFTTFGDMLKVPGPG
                     GESLQKLRAKGADVRVVGSTIEAMRIATDNPGSEVVFMGIGFETTTPTVASMIKTCAR
                     LGVKNMSLYSVHKLVPPAIEALLADSELKVDGFLCPGHVSAIIGTRPYEMIPRKKKAA
                     VIAGFEPVDILEGVSMILEQIITGEFTVENQYGRAVKPHGNTKAVELMEEVFMPAPSQ
                     WRGLGVIPESGLVLRPEYSSFDAIERFDVPYREYEQEEGCMCGHILRGVASPEECPLF
                     KTSCTPHDPVGPCMVSREGTCAAHYRFS"
     gene            complement(4477..4569)
                     /locus_tag="PLT69_05080"
     CDS             complement(4477..4569)
                     /locus_tag="PLT69_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13847.1"
                     /translation="MLCHAGFAIRKVDEVDAEERLALLREILEA"
BASE COUNT          768 a         1608 c         1367 g          835 t
ORIGIN      
        1 ctttgggtac caccttcgtg tcgcccgtca ccaccttcac gccggcctcg cgcgaggccc
       61 tcgccatgga gtcgacgatc ctggccagct cctccatggc aaacccttcc tcgatgatga
      121 acccggcgct caagaacagc ggccttcctg cacaggcagc gatgtcgttg accgtgccgt
      181 gcacggcgag cgacccgatg tcgcctccgg gaaagaaaat cgggtctacg acgaaggaat
      241 ccgtggtgaa acagagcctc ccctcaccca cccgcacgac cgcggcgtcg tccatcctgt
      301 ccaggtgctc gttcgaaaac gaccccacga acatctcccg tatgagcctg ttggtgagca
      361 tgccccccga cccatgttcg aggagaaccg tatccgtctt catgaacatc tcctttcaca
      421 cgttcccata agccaggcgg gacgcagcga ccacggcctg cccgagggat ataccgccgt
      481 cgtttggagg cacccgtgca tgagtggcca ccgagaaccc ctctccttcg agacgggatg
      541 cgcacccctg gaggaggagg cggttctgga agcatccgcc tgagaggacc accgtgccta
      601 tacccgtcat gccccgcaat ctcacggccg tcccggccag ggcttccacg accgtggcat
      661 ggaacgcgga tgcgagcctc gaggcatctt cacccgaaag acgcctttcc acgatcttac
      721 tcacagccgc cgacaggtcg acgaccaggg gtttcctcgt atcgtccacc acgaaaggca
      781 ggacctccgc gtacgtcctg tccgcgcaag cctcgagcac gatcgccgcc tgcccctcgt
      841 acgatgaccg cctgcacagg cccaagaggg ccgagacccc gtcgaagagc ctccccaggc
      901 tcgaggtcac cgggcagttc agccccgact ccagcaaggc ctcgatctgt gccgggtcga
      961 tgcccgcggg aaccaggccc aacctccccg cgatctcctg ccactcctcg ccgaagcacc
     1021 ctctcaggag gctcgccccc gttctccacg gctggcgcac ggccgcctcg ccgccgggca
     1081 gagccacgca cttcaggtgg ccggccctct cgaaggccgt ctcggtcgca atcaggaact
     1141 caccgcccca tatggtggag tcttccccgt acccggtccc gtccatggcc aacccgatga
     1201 ccgggccttt caagcggttc tccgccatgc agctgacgat gtgtgcgtgg tggtgttgaa
     1261 cccggatcac cccggaggag gccttcccca gtgcgtagcg ggtggagaag tagtccgggt
     1321 gcatgtcgca cgcgagaacg ccgggcctgc acctcgctat gcgctgcatg gtctccacgg
     1381 tctcgaggaa gaagtccctg gcgagcggcg tctcgagatc ccccacgtgc ggggacaaga
     1441 aggcgaggtc gtccctcacc acgcacacgg tcgacttgag gtgggccccc agggcaagca
     1501 cctcgggcat ccgagcgccg aggagcaccg gcctcggcgc atagccgcgc gagcgcctga
     1561 cgacggtggg gcccctctct gtcgcgatgg ccaccgagtc gtcgcaccgg accacgatgt
     1621 tccggttgtg caggacaaag gcgtccgcca cgtccttgag cctctcgacg gcttcctcgt
     1681 tgtcgatgca tatgggctcg tccgtcaggt tggcgctcgt catgacgagc accggcgggt
     1741 ccttctccag gatgaggtgg tgcaggggcg tgtacggcag catgacgccg agcgttccca
     1801 tggacggcgc gaccagccgg gacacgggcg agacttccct ctccctgcat atgacgatgg
     1861 gcctggaggg cgacctgagg agcctcctct cctcctctcc aaggtgaacg agcctctcgg
     1921 cggtgaccag atccctcgcc atgaccgcca gtgccttttc ctccctgcac tttctctccc
     1981 tcaacctggc taccgccttg tcgtaaagcg cgttgacgca gagatggaat ccgccgagcc
     2041 ccttgacggc caggatcccg ccttcccgga tcaggttcgc cgcccaggcg aggggatcgt
     2101 ccgtccggat cccgcgaccc tctctgtcca ccagcgtcag ccggggacag cagactgggc
     2161 aggcattggg ctgggcgtgg aagcgcctgt cgcccgggtc ctcgaactcc ttccggcact
     2221 gctcgcacat ggggaagacg gacatggacg tccgctcccg gtcgtacggg acgtcctcga
     2281 cgatggtcag cctcggcccg caatccgtac agttgatgaa cgggtaccgg tacctgcggt
     2341 tcgacgggtc gaacaactcg gccagacatg cgtcgcacgt ggccacatcg ggggagatga
     2401 gggcctcggc ccgccccgtt gcgtcgctcg ccacgatcct gaaagccttc tccccgaggg
     2461 gtgggaccct ctctctcagg acgccgtgta tctcggcaag gggaggggtc ttctccaaga
     2521 gggatgagac gaaagcatcg acgagaccct ccgcgccttc cgcctcgacg gtgaccccgt
     2581 ccgcgtcgtt gcgcacgaac ccggccatgc cgtgggacag cgccgttctg tacacgaagg
     2641 gcctgaagcc cacgccctgg acgatccccg agaaacgcag gcggatcctg gatttcacct
     2701 ggctcaggcg cgccccttcc tgaaggtttc caccctcgtc gtaagccact cgacccaggc
     2761 gtcgagaccc tgccccgtcc tgcacgacac ctcgatgatc acgagctcgg ggttgcatgc
     2821 gagcgcgtcg gccttggcct tgacgatgtc gaactccacg tacggcagca ggtcgatctt
     2881 gttgatgatc atgaccgagg actcgtggaa catgagcggg tacttggagg gcttgtccgc
     2941 gccctcgggg gtggacagga tcatgacctt gtcgttctcg ccgatggtga actcagcggg
     3001 gcacacgagg ttgccgacgt tctcgtttat gaggacgtcc acctcgtcga gcggcagacc
     3061 tggcagggcc tccctgacca tgttggcgtc gatgtgacac ccgccgccgg tgttgatctg
     3121 gaccaccggc acaccgtgcc tcgccaccct ctccgcatcg tgggtgctct ggatgtcgcc
     3181 ctcgatgacc gctatgcggt accttccctt gagggcatcg atcgtctttt ccacgagcgt
     3241 ggtcttgccg gccccggggg agctcataag gttcatcacg tagatcccct tctcgtcgaa
     3301 cagggccctg ttgcctcccg ctatcctgtc gttcgagtcg aggacctgcc tcaccacgtt
     3361 gatcacgggc atcgtcgtct cctttctgtt ctcagctgaa cctgtagtgg gcggcgcacg
     3421 tcccctcccg ggataccatg caggggccca ccgggtcgtg gggggtgcac gaggtcttga
     3481 agagcgggca ttcctcgggg gaagccaccc ctctcaggat gtggccgcac atgcaaccct
     3541 cctcctgctc gtactctctg tacgggacgt cgaatctctc gatcgcgtcg aaggaagagt
     3601 actcgggcct gagcacgagc ccgctctccg gaatgacacc gaggccgcgc cactgggaag
     3661 gcgccggcat gaacacctct tccatcagct ccacggcctt cgtgttgcca tggggcttca
     3721 cggcacgtcc gtactggttc tcgaccgtga actcaccggt tatgatctgc tcgaggatca
     3781 tggatacccc ctcgaggata tccaccggtt cgaacccggc gatcactgcc gccttcttct
     3841 tccgcggtat catctcgtac gggcgggtgc cgatgatcgc gctcacgtgc ccagggcaga
     3901 ggaagccgtc caccttgagc tcgctgtcgg ccagcaacgc ctcgatggcg ggggggacga
     3961 gcttgtgaac ggagtacaat gacatgttct tcacgcccaa tctcgcgcac gtctttatca
     4021 tggaagccac ggtgggggtc gtggtctcga aacctatgcc catgaacacg acctcgctgc
     4081 cggggttgtc cgtcgctatc ctcatcgcct cgatggtgga cccgacgacg cgcacgtcag
     4141 cccccttcgc cctcagcttc tggaggctct caccgcccgg gcccggcacc ttgagcatgt
     4201 cgccgaaggt ggtgaagagc acccccttct tccccgccat gtcgagcgcc ctgccgacat
     4261 cggatgccga cgtcacgcac accgggcatc ccggccccga gatcaggcgc acgttctcgg
     4321 gcaggatcct cctgatcccg tacctgccta tggcgtacgt gtgcgtcccg cagacctcca
     4381 tgagggtgac gcctctcccg atgccgcggg cctggcgatg gatgcgttcc ctgaggcgca
     4441 cgagcagctc gttgtcccgg tactcgtcga tcagcttcac gcttcgagaa tctccctgag
     4501 cagggcgagg cgttcctcgg cgtccacctc gtccaccttc cggatggcga accccgcgtg
     4561 acacagcacg aagtcgcc
//
LOCUS       DAOSCR010000152         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11917_length_4576_cov_3.423358, whole genome shotgun sequence.
ACCESSION   DAOSCR010000152 DAOSCR010000000
VERSION     DAOSCR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11917_length_4576_cov_3.423358"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..888
                     /locus_tag="PLT69_05085"
     CDS             154..888
                     /locus_tag="PLT69_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOJ13848.1"
                     /translation="MARYLEERGVGLDLVVARVPVVPTAVIYDLSFMDPKARPTPRMA
                     YEACLAATSDPVEQGCVGAGTGATCGKLRGVLNATKSGLGSSLVHGADGVCVAALVVA
                     NPFGDVLDRTGRIVAGARDGGTFLDMAGAIRKGEVRQRFGAPGNTTLCVVVTDALLDK
                     VSAMQVARMAGQGLARHIVPYNTPLDGDMVFCLSVGDKKAHPLHLGVLAQDAAREALL
                     NAVWRACSMGGLPAARDLVDDACMVP"
     gene            955..1031
                     /locus_tag="PLT69_05090"
     tRNA            955..1031
                     /locus_tag="PLT69_05090"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:989..991,aa:Met,seq:cat)
     gene            3358..>4576
                     /locus_tag="PLT69_05095"
     CDS             3358..>4576
                     /locus_tag="PLT69_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HOJ13849.1"
                     /translation="MRPKILLAFCVHSHQPVGNYDSVFEQGTNDCYIPFLRILREYPD
                     IRMTLHYTGPLFEWFEKSSPEFFEILSELVARSQVELMGGGFYEPILSVIPERDAVSQ
                     IEYTSRYLVDRFGVRPTGMWCTERIWDPSLPKKIRAGSLVEYTLLDDSHFLSAGLCPE
                     DVHGYFMTEREGYALKVFPIDMRLRYLIPFKEPHETIEYLLRLRSRGVEVLTYGDDGE
                     KFGMWPGTRMWVMEQGWLRRFFDAMLGVKDKIELVPLSEAIARTRPRGLVYLPTATYQ
                     EMMEWSLLAPQGRYYEELVKQAKAQHDWERKRAFLRGGMWDNFLSKYRASNLMHKKML
                     RVSELVDHHVGPTEAKRRLFMGQCNCAYWHGLFGGIYVGALRHAVYENLLAAESLVDE
                     KRFSSSRWSVERSD"
BASE COUNT          857 a         1413 c         1466 g          840 t
ORIGIN      
        1 ccattttgga tgggttttgt taggaaaagg gcgctggggt cagctccgcg ccttcagacg
       61 ggcctgcacc gcgtcgccgg tgagctcgcg caaggcgtcg gcggcaatcc agcgcgcggc
      121 gcgcgaaccc tgcgcctgga tgcggcgtgc ggcgtggcgc gctacctcga agagcgtggc
      181 gtggggctcg acctcgtggt ggcccgtgtg ccggtggtcc ccaccgcggt gatctacgac
      241 ctctccttca tggaccccaa ggcccgtccc acgcccagga tggcctacga ggcgtgcctg
      301 gccgcaacgt ccgacccggt ggagcagggg tgcgtcggcg cagggaccgg ggccacgtgc
      361 ggcaagctgc gcggcgttct caacgccacc aagagcgggc tgggttcgag cctggtgcat
      421 ggagcggacg gggtctgcgt ggccgcgctc gtcgtcgcga accccttcgg cgacgtcctc
      481 gacaggacgg gcaggatagt ggccggtgcg cgggacggcg ggacgttcct ggacatggcc
      541 ggtgccattc gaaaggggga ggtcaggcaa cgcttcgggg cgcccggcaa caccacgctg
      601 tgcgtggtgg tgaccgacgc cctgctcgac aaggtgtccg ccatgcaggt ggcacgcatg
      661 gcaggccagg ggctcgctcg ccacatcgtg ccgtacaaca cccccctgga cggggacatg
      721 gtgttctgcc tgtccgtggg agacaagaag gcccacccgc tgcatctggg cgtgctcgcc
      781 caggatgctg cccgtgaggc gctcctgaac gccgtgtgga gggcatgttc catgggcggt
      841 ctcccggcgg ccagggacct cgtcgacgac gcctgcatgg tgccttaggg caagggatcg
      901 agtcacccaa aaacgttgac aaaggggtgc ctcttgggta gaagacatga ccgtggcgga
      961 gtagctcagg tggtcagagc atgcggctca tatccgcagt gtcgggggtt caaatccctc
     1021 ctccgctacc acccccccaa taaaggtatc gtaggcctcg aacacggttg agcacccccg
     1081 ttcaaaacac ccgtctgcaa cgaaccaccc ggtcgtcacc acccctgctc gagctgcgcc
     1141 catcctgagg ccgatgacaa gcgcgtgcat ggatccgaga cctctcctcc cggtagcggc
     1201 agggcttggc ctgtctcttt cgccgtatgt cgcgccgtgt atctctcgag cgccgggatc
     1261 atcctcgtga agggcgggag accggctctc gagcgttccc gcggtaggcc catcgatcga
     1321 agccaaggcg caggcgccct tcgggtcgcc tggccacccc gacaacctcc acggtgcctc
     1381 gcagcggcct cagcggtgaa gccctcgagg atctcccctg ccagctgggt tcaccctcga
     1441 caccctcggt gctttttacg cacagagtac ggcttcagag gttcaccttg ggctccggcg
     1501 cggggctcaa cctcgacacc ctcggtgctt gcgctcccgt catggccggg atgcgcgaag
     1561 gcggcacgat gagtcctctg cgggggctcg gcgaggcgtt ctgcgacgct gcgccacggt
     1621 gggggcggac aagctcggcg tcgttttcgc gaggatggat tcgtgagcag ggatgccctt
     1681 agacggcaaa accacaagaa agaggccctg gctggtcctc gggaaagggc gcgtcacgag
     1741 ggcgcagact tgatatgccg ctcaaatccg accaggttca tgcgcaggcc ggatcttgca
     1801 gcaacagggt gcaaggcctt tccccgggtc ggacggcatc ccgcccgggg cttgtcaagc
     1861 ctacctgaag agcctccttg cagacgccaa gccgatgagc cctgacccca gaagaagcat
     1921 ggacgcgggc tcagggaccg gggccggtgc aggctcctct ccctggaccg ggttcccccc
     1981 atcatacccg gtatctccgc cgaccggagg gtatgttccc ggggttgggc cgctgtcgtg
     2041 gtgaggttga tggtaggggt attcaggtct ccagtggttg tccccctcgc tcggcccggc
     2101 cgggaagggc atgtacggcc cacccgagga aaagccccag gagaaccaga aggcggagct
     2161 ttggttgctt gtccaccctc tcgtcgtgcc gacgtccgtc ccgctgagag gaagagcgta
     2221 tgccgtgaat ggggccgcaa agaggatcgc cgttatgata gccagatatt ttttcatcct
     2281 cacctctctt tcctctcgac cgagagcctt cccctgtcat ctatcgtgaa aacaccgtta
     2341 ctcgtataat aatccacatg gaggaaaaag caaagacaat gccaagccat tttccttgag
     2401 gagagggaaa cacgacggat aatcagagca ttaccaaagg cacatctctt aaatgctata
     2461 atatggaaat atactaccac tattggagat ttgtttgccc aatggccctc tcctccaggg
     2521 agggagaccc gggcaagact cgtggtcttg atgtcacggt gtgtgtatcg agcatggcgt
     2581 tgtctggggt gaagcaggcg ggtcgtctcg tgcttggggt aaaggcctga acagcccgtg
     2641 cagcgacgac tctgagaccc aatgaacacc gtgatctcgg ttctgccagg accgtggatc
     2701 cggcatgacg ctcggcgtga tcctcccttc cggggatggg aaggccgccg tcgtacgccg
     2761 tgtcatcacg acgatccggc agatcattcc ttacgggtat gggaagacct cacgaaggca
     2821 tggtgcgtgg gtgcgccgat tggatcctcc caaaggggga tgggaagaac gattcgtcgg
     2881 cgaccatcct caacagcatc gccaggtctt cccttatggg gagggaaaga ccgcaagcgg
     2941 gagagcgtgt tgccaggcag ggcctgagac tgtcgccccg tgtccttccg cccgataagc
     3001 ctgcctgctc cccctttctc tcttgcatgt ccatggagcc ccacggtcac ccccctcgag
     3061 aaggctgcac ccggccgatt tcctcaccca agggggtgtt cgaccgtatg gtttgcttcc
     3121 acggcggcaa aaccctgcca agttcctctt cccctccggt gcccgtttcc gtataaggag
     3181 agtgcacagg ggccatgtcc ttaccggacg gcatggatcg aaaaaacgtc gagggatggg
     3241 gcaagaccca gggaaaggct ccgcctgtga tctttcacgt gctgcatgaa caggccccat
     3301 gccgaggaga agccgcctgt gtccgataga tagagatccg ggtggaggtg atcccgtatg
     3361 cgaccaaaga ttctcctggc cttctgcgtg cactcgcacc agcccgtggg aaactacgac
     3421 tcggtcttcg aacagggaac gaacgactgc tatatcccct tcttgagaat tctcagggaa
     3481 taccccgaca tcaggatgac cctccactac accggcccgc tcttcgagtg gttcgagaag
     3541 agttcccccg agttcttcga gatcctctcg gaactcgtgg cgaggtctca ggtggaactc
     3601 atgggtgggg ggttctacga gcccatcctc tcggtgatcc ctgaacgcga cgcagtctcg
     3661 cagatcgagt acacctcgcg ctaccttgtg gaccggttcg gcgtgagacc cacgggcatg
     3721 tggtgcacgg agcgcatatg ggacccgagc ctgccgaaga agatcagggc gggatcgctc
     3781 gtggagtaca cgctgctcga cgacagccac ttcctctctg caggcctgtg ccccgaggat
     3841 gtacacggct acttcatgac cgagcgcgag gggtatgccc tcaaggtgtt ccccatcgac
     3901 atgcgccttc ggtacctgat cccgttcaag gagccccacg agacgatcga gtatctcctc
     3961 aggctccgct cgcggggagt ggaagtcctc acctacggag acgacggcga gaagttcggc
     4021 atgtggcccg gaacccgcat gtgggtgatg gagcagggct ggctcaggag gttcttcgac
     4081 gcgatgctcg gggtgaaaga caagatagag ctcgtccccc tgtcggaggc gatcgccagg
     4141 acgaggccga gggggctcgt ctacctgccc acggcgacct accaggagat gatggagtgg
     4201 agcctcctcg caccccaggg aaggtactac gaggaactcg tgaagcaggc caaggcccag
     4261 cacgactggg aacgcaaacg ggcctttctt aggggtggga tgtgggacaa cttcctgtcc
     4321 aagtaccggg cctccaacct catgcacaag aagatgctca gggtgagcga actcgtagac
     4381 caccacgtgg gtccaaccga ggcgaaaagg aggctcttca tgggccagtg caactgcgcc
     4441 tactggcacg ggctcttcgg cggcatctac gtgggcgcct tgaggcacgc ggtgtacgag
     4501 aacctccttg cggcggagtc cctcgtcgac gagaagagat tctcctcttc acggtggtcg
     4561 gtggaacgct cggacc
//
LOCUS       DAOSCR010000153         4574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11926_length_4574_cov_3.264218, whole genome shotgun sequence.
ACCESSION   DAOSCR010000153 DAOSCR010000000
VERSION     DAOSCR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11926_length_4574_cov_3.264218"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLT69_05100"
     CDS             <1..605
                     /locus_tag="PLT69_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOJ13850.1"
                     /translation="PFTLVAAKVALVLPLALLTAAFNPFYDRQPVVTVFDLPVTGGMI
                     SFVSIIVKFVLTAAGAVTLVAVTGFNDLCAAMGRLGVPNVLTTQLLLLHRYLFTLSAE
                     AAHMRRARDLRSPGTDAHGIRTASAVLGHLLLRTYARAERIHLAMLSRSRRGDILPHR
                     SFRSRPADWVFCVLTCALFIAWRVMDMTSALGSLALGVLG"
     gene            602..1360
                     /locus_tag="PLT69_05105"
     CDS             602..1360
                     /locus_tag="PLT69_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13851.1"
                     /translation="MSHHIVELKDVTYVYPDGTEALKGVSLKITHGEAVALVGANGAG
                     KSTLLLNLNGCLMPTSGSVRIGDYPVTQATLANVRRTVGTIFQDPDDQLFMPTLGDDV
                     SFGPYNLGLRGEELRRRVDEALARMGIEHLRNRPPYRLSAGEKRRGAIASVLAMTPDI
                     LILDEPTTGLDPRGRRQMIEILKGFAHTRIIATHDLDLVLEVCPRTVVLSEGSIRADG
                     PTLEIFRDADLLERCSLEPPLSLKGCPVCGKGPG"
     gene            2076..2912
                     /locus_tag="PLT69_05110"
     CDS             2076..2912
                     /locus_tag="PLT69_05110"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOJ13852.1"
                     /translation="MTGLTTSRTSYDGGTSVLKWPVEGVMPSMRARLSFRETVDASIQ
                     VTATPWYSQDSRMRDFDYLDESEWNRQAHEGVDIYSETETEAKTVELSTHLRFLPVCT
                     GFMRAGLTAGYSRREHDFRGFDTIQTGHGPWVDQARTLKGPSTLYSVKYEIYSAGVAV
                     EWTCFESLAVALEWASLPVVHATDEDDHLRRNRAGRSSTRGSGYRTSVYALAPVGRRL
                     AVTMSCTHERFDTDGRQTQYWYGDDPATPGFNDTGYGLGGIRTDLDSRCTTLSIGSRV
                     AF"
     gene            2991..4007
                     /locus_tag="PLT69_05115"
     CDS             2991..4007
                     /locus_tag="PLT69_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ13853.1"
                     /translation="MRSYSSLKILVVGTGTFYHHNLENMLTKIGFPAPEHADDGPSGW
                     EAVKRLMPDIVVAQWDMPEMNGLALLKVIRSDPFHGETPVILCTNEITKSDVIKAGES
                     GVNAILVEPLSIEVLEQKINLILEFDMTPSTKQARAYLEKGDRYMRAGLFDLALKEYE
                     RVLTILESAEVYYNIGYIKTARGEYDEALVAFRKATQINQLFAKAYQAMAEVYIKLGD
                     KAMAEKYLNMAGEIFLEREMHEKAESVFNEIIALNPETTNVYNSLGILYRRQNRHADA
                     VKVYQKAVKIDPEDENIHFNMGRAYLEMGQHELARQCFKKALELNPRFDTAQAMLKAL
                     ESID"
     gene            complement(4138..>4574)
                     /locus_tag="PLT69_05120"
     CDS             complement(4138..>4574)
                     /locus_tag="PLT69_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ13854.1"
                     /translation="TAHYIQALQEDCTIVPLKEFNEIDAGICEGMTYDEIQRLKPDVY
                     AARKADKYNYTYPLGEGYATMKERIKIGIKKAFFLNQKPDNIMIVGHRAVNRMILSHF
                     LYRREEDVPYIYVPQDKFYHIVATQDRKVFELKRYDFRRAGR"
BASE COUNT          849 a         1521 c         1432 g          772 t
ORIGIN      
        1 tccccttcac cctcgtcgcc gcgaaggtcg cgctcgtcct cccgctggcg ctattgaccg
       61 cggccttcaa ccccttctac gaccgccagc ccgtcgtcac cgtcttcgac ctgccggtca
      121 cgggcggcat gatctcgttc gtgtccatca tcgtcaagtt cgtcctcact gcagccggcg
      181 cagtcaccct cgtggcggtg accggcttca acgacctgtg cgcggccatg ggcaggctcg
      241 gtgtcccgaa cgtcctcacc acccagctcc tcctcctcca ccgctacctc ttcaccctct
      301 cggcagaggc cgcccacatg aggcgcgcga gggacctgcg ttcccccggg accgacgccc
      361 acggtatcag aaccgcatcc gcggtcttgg gccatctcct cctgaggacg tacgcgcgcg
      421 ccgaaaggat ccacctcgcc atgctctcga ggtccaggcg aggcgacatc ctgcctcaca
      481 ggtctttcag gtcgagacca gcggactggg tcttctgtgt cctcacgtgc gccctcttca
      541 tcgcttggag agtcatggac atgacgtccg ccctgggaag cctcgcgctg ggggtgctcg
      601 ggtgagccat cacatcgtcg agctcaagga tgtcacgtac gtgtaccccg acgggaccga
      661 ggccttgaag ggggtctcgc tgaagatcac gcacggcgag gccgtcgcgc tcgtgggtgc
      721 caacggcgcg ggcaagtcca ccctgttgct caacctcaac ggctgcctca tgcccacctc
      781 gggcagcgtc cgcatcggcg actatcccgt gacgcaagcg acgctggcga acgttagacg
      841 aaccgtgggc acgatcttcc aggaccctga cgatcagctc ttcatgccga ccctcgggga
      901 cgacgtgtcg ttcgggccct acaacctggg cctcaggggc gaggagctca ggcgcagggt
      961 ggacgaggcg ctggcgcgca tgggcataga gcacctcagg aataggccgc catacaggct
     1021 gtccgcgggc gagaagaggc ggggcgcgat cgcctccgtc ctcgccatga cccctgacat
     1081 cctcatcctc gacgagccta ccacgggtct cgacccgcgg ggccgcaggc agatgataga
     1141 gatcctcaag gggttcgccc acaccaggat catagccacc cacgacctcg atctcgtcct
     1201 tgaggtatgc ccgaggaccg tggtgctctc ggaagggtca atccgcgctg acggccccac
     1261 cctggagatc ttcagggatg cggacctcct cgagagatgc agcctcgagc ccccgctctc
     1321 gctcaagggt tgcccggtgt gcggaaaagg tcccgggtga acccggctcc acgggactcc
     1381 gacacaggac gcctgccagg gcctccccat gacgacgacc tgaaacggcc cacacccggc
     1441 tcaacccccg ccggaggaag tgagggaaag ggcaaggcac ggtgagttcc cggtgcccat
     1501 cgtgcggcca gggcgtagtc tcctcgacgg cgggccacgg ccgacacccg cctttaccct
     1561 ctgccagggg aggtgcggga aaggacaagg tcaccatgaa cgcccgatac ccctcaggca
     1621 gggcgtagcc tcctcgacga cgggcgacgg cccacacccg cttgcccacg agaggcgagg
     1681 caaggggcac ggcacggtga gtgcccggtg cgttcagacc tgtttcctcc ggaaaggctc
     1741 gagccccacc catgacctgg ccctggccac cttcagggcg ccctttgagc ccgttttccc
     1801 ttgccgtcac aaaacccccc gtgatatcta tgaaatagat gatgcgcggt ccaggacacc
     1861 atgacgagga acaaggcatg cccgcttcct ttccggatga cgcccacggg tgtgaggccg
     1921 gggctttccc gcaccgacgc accggttccc gcaggaaaca cctgggcagc cttttcgcgg
     1981 ggtggtcggc acttctgctc gccctgctgt cgcctggcct ttgcccttcc catgccatgg
     2041 acctcgatgg gagcatggag gtgtcctgcg tgtccatgac aggcttgacc acctcccgca
     2101 cctcgtacga cgggggtaca agcgtcctca agtggccggt ggagggggtg atgccgtcga
     2161 tgcgcgcgcg cctttctttc cgggagaccg tggatgcaag catccaggtc acggccacgc
     2221 cgtggtactc ccaggattcc aggatgaggg acttcgacta cctggacgag agcgagtgga
     2281 acaggcaggc gcacgaaggg gtggacatct actcggagac cgagaccgag gcgaagaccg
     2341 tggaactgtc aacgcacctc aggttcctcc ctgtctgcac agggttcatg agggcgggcc
     2401 tcaccgcggg atacagccgc agggagcacg acttcagggg tttcgacacg atacagacag
     2461 gccacggtcc ctgggtcgac caggcgcgca cgctcaaggg gccgagcacc ctctacagcg
     2521 tgaagtacga gatctactcg gccggggtgg ccgtcgagtg gacttgcttc gagtccctcg
     2581 ccgtcgccct ggaatgggcc tccctgcccg tcgtccacgc aacggacgag gacgaccacc
     2641 tgaggcggaa ccgggcgggc agatcgagca cgaggggatc gggatacagg acctcggtgt
     2701 acgccctggc cccagtcggc cgaaggctcg ccgtcacgat gtcctgcacc cacgagcgct
     2761 tcgacacgga cgggcgccag acccagtact ggtacggcga cgatcccgcc acaccgggtt
     2821 tcaacgatac gggctatggt ctcggcggca tcaggacgga cctggactcc aggtgcacga
     2881 ccctgtccat aggctcgcgt gtcgcgttct gacgcccatc ggcgcgcggg gaggcgggca
     2941 tggcttgcgg caggcgtcgt cgccgcttat aatcatgaaa aaacagggcc atgagatcat
     3001 actcctcgct caagatactc gtcgtgggaa ctgggacgtt ctaccaccac aacctcgaga
     3061 acatgctcac caagatcggc tttcccgccc ctgagcacgc agacgacggg ccttccgggt
     3121 gggaggccgt gaagcgcctc atgccggaca tcgtggtcgc ccagtgggac atgccggaga
     3181 tgaacggcct ggcgctgctc aaggtcatcc ggtcggaccc cttccacggc gagacccctg
     3241 tcatcctctg taccaacgag atcaccaaga gcgacgtgat caaggccggg gagtcgggcg
     3301 tgaacgccat cctcgtggag cccttgagca tcgaggtcct cgagcagaag atcaacctca
     3361 tcctcgagtt cgacatgacg ccgtcgacga aacaggcaag ggcctacctg gaaaagggcg
     3421 acaggtacat gagggcgggg ctgttcgacc tcgcgctcaa ggagtacgag cgggtgctca
     3481 ccatcctcga gtcggccgag gtctactaca atatcggcta catcaagaca gcccgggggg
     3541 aatacgacga ggccctggtg gcgttcagga aggcgacgca gatcaaccag ctcttcgcga
     3601 aggcatacca ggccatggcc gaggtgtaca tcaagctcgg cgacaaggcc atggccgaga
     3661 agtacctcaa catggcgggg gagatcttcc tcgagcggga gatgcacgag aaggccgaga
     3721 gcgtgttcaa cgagatcatc gcgctcaatc ccgagaccac gaacgtgtac aacagcctgg
     3781 gcatcctcta ccgcaggcag aaccgacacg ccgacgcggt caaggtgtac cagaaggccg
     3841 tcaagatcga ccctgaggac gagaacatcc acttcaacat ggggagggcg tacctggaga
     3901 tgggccagca cgaactcgcg aggcagtgtt tcaagaaggc cctcgagctc aaccccaggt
     3961 tcgacaccgc ccaggccatg ctcaaggcgc tcgagtccat cgactagccc cccgggcgcc
     4021 taccgggcgc ggatgcgtga tcaccgcacg gcccggctcc cgtctctctg gcggcccggg
     4081 gaaggccgga ggccacccgc cagaggggat ccgggcagcc acgccgggcg cttgcctcta
     4141 tcggccggcc ctcctgaagt cgtacctctt cagctcgaac accttcctgt cctgggtggc
     4201 cacgatgtgg tagaacttgt cctgggggac gtagatgtac ggcacgtctt cctccctgcg
     4261 gtagaggaag tgcgacagga tcatgcggtt gaccgccctg tgcccgacga tcatgatgtt
     4321 gtcgggcttc tggttcagga agaaggcctt cttgatgcct atcttgatgc gctccttcat
     4381 ggtcgcgtac ccttcgccca gggggtaggt gtagttgtac ttgtccgcct tgcgcgcggc
     4441 atagacgtcg ggcttcaggc gctgtatctc gtcgtaggtc atgccctcgc agatacccgc
     4501 gtctatctcg ttgaactcct tgaggggcac gatcgtgcag tcctcctgga gggcctggat
     4561 gtagtgggcg gtct
//
LOCUS       DAOSCR010000154         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11945_length_4568_cov_3.582982, whole genome shotgun sequence.
ACCESSION   DAOSCR010000154 DAOSCR010000000
VERSION     DAOSCR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11945_length_4568_cov_3.582982"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1114)
                     /locus_tag="PLT69_05125"
     CDS             complement(68..1114)
                     /locus_tag="PLT69_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ13855.1"
                     /translation="MGGSFQPKKFRYLSDPPVETRPFSIEVDQEKCTGCGMCVRQCPT
                     QSIELVDKKPRFTGLPYFGKICIACHNCEAVCPTGALVFNHFYRVDKGRWAYDFDYPE
                     GHGDGMPNPLMTERPADFSALEDKLTPVEKVIYTRRSVRVYKDKPVPKELIHRVLEAG
                     RFAPSAGNCQGWKFTVVTNKELLNQISASTLKFLNLFTKLYQGKGPVRTFLKKTLAIV
                     KPEGIDQRPMVAIQALLTPKFKKQPLSVFFDAPCAIFLIAHHMHISDASLDIGICAQN
                     MVLAAHSLGLGTCYVGFVSTALKMDPATKKFWPALGIGWPYDNPCTVLTLGYPATRVD
                     KAVDREKPRVMWVE"
     gene            complement(1195..2433)
                     /locus_tag="PLT69_05130"
     CDS             complement(1195..2433)
                     /locus_tag="PLT69_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOJ13856.1"
                     /translation="MSDVYIIGVGMIRFGKYLDGSVRGMAKEAFDLVLKDAGIPKEAI
                     EAAYVSNTFWGMFSNQHSIKGEVMLWDIDMNGIPIVNCENACAGASTAVHLGYTAIKA
                     GMYDVVLALGSEKITHQNKALSLSAYATCMDVENLESHINMFMELTKKLDFRLPDGET
                     PPGEGRSIFMDAYAMGARWHMKKYGSTKRQLAVICSKNHFHGSLNPKAQYQEPMTVDE
                     VMNARPVTYPLTVPMCAPVGDGAAAAILCSERFLRKLSGARPVRIRASVLGSGTHRDL
                     DGEDIGERLSKKAYEIAGVGPSDIDVAELHDATSYGELHQYEAMGFCKPGEGGILAES
                     GATTLGGRIPVNTSGGLECRGHPIGASGLAQIHEIVEQLRGEAGKRQVEGARIGLTEN
                     GGGNLGVEEAAMCIHILEKA"
     gene            complement(2492..3757)
                     /locus_tag="PLT69_05135"
     CDS             complement(2492..3757)
                     /locus_tag="PLT69_05135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13857.1"
                     /translation="MAYDYLDLQCADQIVLNDDHEALKKQVRKFVNEVVKPASIELDK
                     MSALDVIKEGSPYWTVMRKMHELGYHTIFIPEELGGIGLDPLGLHIFFEEMAAGSIGL
                     AVACGVDVFPSFFTSLILPDYPELEELIIQPYVADRDCSLLGCWAITEPEHGSDILTP
                     YQDHFHNPRITHQLVGKPDGDDWILNGQKAAWVSNGTTATMALLFFGTDPSKGLAGGG
                     IAIADLTVPGVSRGKALEKIGQRDLPQGEIYFDDVRVPKERIVCTADAYEQMTDLVLG
                     AANAHMAVMCLGGARAAFEETLTYTRERVQGGKPLCEHQDIQRRLAEMFIEIEAARQL
                     SRNVLNYNLWSFPPHTTYSMVSKIFCSRTAFDVAQNALQIFGGNGLSKEYHIEKLFRD
                     ITSTRIEDGSNETLTLAVGHEILFGERPY"
     gene            complement(3798..>4568)
                     /locus_tag="PLT69_05140"
     CDS             complement(3798..>4568)
                     /locus_tag="PLT69_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13858.1"
                     /translation="MMGLYSEPRRPDDADNPVRVVLSAGTPRSIWEDFEKRFNVKIHE
                     WYGAVEGGFAHNPPGVGPIGSFGKPIEGIMEMKVVREDDTECAPGEIGELIVVPKQGK
                     AEVEYLGKKKASEEKTRGGVLRTGDMCHRDENGWFYFDFRKGGGLRRHGDFILPEYVE
                     KALAELPEVSDVCVYGIPAETGAPGESDIVAAVVLVPGVALDLGKVRSALEKSLEKNS
                     IPQFLQVVEEIPKSASEKNLDRLLKEEFRKDAPNVYRL"
BASE COUNT          723 a         1510 c         1380 g          955 t
ORIGIN      
        1 atgaccctga acccgcccgc tccttgtcga cgaaggccga cctgtcccat gcgggtcccc
       61 gggcggctca ctccacccac atgaccctcg gcttctccct gtccacggcc ttgtccaccc
      121 tggtcgcggg gtagcccagc gtcaacacgg tgcaggggtt gtcgtacggc cacccgattc
      181 ccagcgcagg ccagaacttc ttcgtcgccg ggtccatctt gagggcggtg gagacgaacc
      241 ccacgtaaca cgtcccgagg ccgagcgagt gcgccgccag gaccatgttc tgcgcacaga
      301 tgccgatgtc cagcgaggca tcggagatgt gcatgtggtg tgcaatgagg aagatggcgc
      361 acggcgcgtc gaagaacacg ctcaggggct gcttcttgaa cttcggcgtg agcagggcct
      421 gtatggccac catgggcctc tggtctatcc cctcgggctt cacgatggcg agcgtcttct
      481 tgaggaaggt cctcacgggg cctttgcctt ggtagagctt cgtgaagagg ttcaggaact
      541 tcagcgtgga ggcgctgatc tggttcaaca gttccttgtt cgtcaccacc gtgaacttcc
      601 atccctggca gttgccggcc gacggcgcga acctcccggc ctcaagcacc cggtggatga
      661 gttccttggg gacgggcttg tccttgtaca cgcggacgga tctcctcgtg tagatcacct
      721 tctccacggg ggtgagcttg tcctcgagag cggagaagtc cgctggcctc tccgtcatga
      781 gcgggttcgg catgccgtcc ccgtggccct ccgggtagtc gaagtcatag gcccacctgc
      841 ccttgtccac ccggtagaag tggttgaaga cgagggcccc cgtggggcag accgcctcgc
      901 agttgtggca ggcgatgcag atcttcccga agtaggggag acccgtaaag cggggcttct
      961 tgtccacgag ctcgatggac tgcgtcggac actgcctcac gcacatgccg catcccgtgc
     1021 acttctcctg gtcgacctcg atggaaaacg gcctcgtttc caccggcgga tcgctcaggt
     1081 acctgaactt cttcggctgg aaagatcctc ccatacgcgt cccctttcct ttcctgtgtc
     1141 atcgggcccg aaaaggcgca tcttgcgcct tccgggcccc ccatgcactt tgtctcaggc
     1201 cttctccaga atatggatgc acatggcggc ctcttccacg ccgaggtttc ccccgccgtt
     1261 ctcggtgagc ccgatgcgtg cgccttcgac ctggcgcttg ccggcctctc ccctgagctg
     1321 ctccacgatc tcgtgaatct gcgcgaggcc ggaggcgccg atggggtgtc ccctgcactc
     1381 gaggccgccg ctcgtgttca ctggtatcct tccgccgagc gtcgtggcac ccgactcggc
     1441 gaggatcccg ccctcgcccg gcttgcagaa ccccatggcc tcgtactggt ggagctcccc
     1501 gtatgacgtg gcgtcgtgga gctccgccac gtcgatgtcc gacggcccga cgccggcgat
     1561 ctcgtaggcc ttcttggaga gcctttctcc tatgtcctcg ccgtcgaggt cacggtgcgt
     1621 cccggatccg agcaccgagg ccctgatcct caccgggcgc gcgccggaga gctttctcag
     1681 gaacctctcg gagcacagga tcgcagccgc agcaccgtca cccacagggg cgcacatcgg
     1741 caccgtcaag gggtaggtga cgggcctggc gttcatgacc tcgtccacgg tcatgggttc
     1801 ctggtactgg gccttggggt tgagcgatcc gtggaagtgg ttcttcgagc agatcaccgc
     1861 gagctgccgt ttcgtggatc cgtacttctt catgtgccac cgtgcaccca tcgcataggc
     1921 atccatgaag atgctcctgc cctctccggg gggcgtctcc ccgtccggca gccggaaatc
     1981 gagcttcttg gtgagctcca tgaacatgtt gatgtgactc tcgaggttct cgacgtccat
     2041 gcacgtcgcg taggcgctga gagagagggc cttgttctgg tgtgtaatct tctcggagcc
     2101 gagcgcgagc accacgtcgt acatccccgc cttgatcgcg gtgtagccga ggtgcacggc
     2161 ggtggaggcc ccggcgcagg cgttctcgca gttgacaatg gggatgccgt tcatgtcgat
     2221 gtcccagagc atgacctcgc ccttgatgga atgctggttg gaaaacatgc cccagaacgt
     2281 gttcgagacg taggccgcct cgatggcctc tttgggtatc cccgcgtcct tcaggacgag
     2341 gtcgaaggcc tccttcgcca tgcccctcac ggatccgtcg aggtacttgc cgaacctgat
     2401 catgcccacg ccgatgatgt acacgtcact catggatgcc tcctggcgat gaaagtgtct
     2461 cctgtgcctg caacccggct ccccatgtgt ctcagtacgg ccgctcgccg aagaggatct
     2521 cgtgccccac ggcaagcgtc agggtctcgt tggatccgtc ctcgatcctc gtggacgtga
     2581 tgtccctgaa gagcttctcg atgtggtatt ccttggagag cccgttccct ccgaagatct
     2641 gcagcgcgtt ctgggcgacg tcgaaggctg tgcgcgagca gaagatcttg gacaccatcg
     2701 agtacgtggt gtgcggcggg aagctccaga ggttgtagtt caggacgttc ctggagagtt
     2761 gcctggcggc ctcgatctcg atgaacatct ccgcgaggcg cctctggatg tcctggtgct
     2821 cgcacagcgg cttgccgccc tggacacgtt ccctcgtgta ggtgagcgtc tcctcgaacg
     2881 cggccctggc tcctcccagg cacatgaccg ccatgtgggc gttcgccgcc cccagcacga
     2941 ggtcggtcat ctgctcgtag gcgtccgcgg tgcagacgat gcgttccttc ggcaccctca
     3001 cgtcgtcgaa gtagatctcc ccctgcggca gatctctctg gccgatcttc tcgagcgcct
     3061 tgcccctgga cacgcccggg acggtcaggt cggcgatcgc tatccccccg cctgcaagac
     3121 ccttggacgg gtccgtgccg aaaaacaaca gggccatggt cgccgtggtc ccgttcgaga
     3181 cccatgcggc cttctggccg ttcaggatcc agtcgtcgcc atcgggcttg cccacgagct
     3241 ggtgggtgat cctggggtta tggaagtggt cctggtaggg cgtgaggatg tcggagccgt
     3301 gctcgggctc ggtgatcgcc cagcatccca gcagggaaca gtccctgtcg gccacgtagg
     3361 gttgtatgat gagctcctcg agctccgggt agtcgggcag gatgagcgag gtgaagaacg
     3421 aggggaacac gtccacgccg caggcgaccg ccagccctat gctgcccgcc gccatctcct
     3481 cgaagaagat gtggagcccc agcgggtcga gccctatgcc tcccagttcc tcgggtatga
     3541 agatggtgtg gtagcccagc tcgtgcatct tgcgcatgac cgtccagtag ggcgacccct
     3601 ccttgatcac gtcgagggcg ctcatcttgt cgagctcgat ggatgcgggt ttcaccacct
     3661 cgttgacgaa cttcctcacc tgtttcttga gggcctcgtg atcgtcgttc aggacgatct
     3721 ggtctgcgca ctgcaggtcg aggtaatcgt aagccatctg tttctcctcc ttcagcgatt
     3781 gtcaaactgc gaacctgtca cagcctgtaa acgttcggtg cgtcctttcg gaactcctcc
     3841 ttgagaagcc ggtcgagatt cttctccgag gcagacttgg ggatctcctc cacgacctgc
     3901 aggaactggg ggatggagtt cttctccagg ctcttctcga gggccgaccg taccttgccg
     3961 aggtcgagcg ccaccccggg gacgagcacg acggccgcga cgatgtcgct ctcgcccggc
     4021 gcgcccgtct cggcggggat gccgtaaacg cacacgtcgg acacctcggg gagttcggcc
     4081 agcgccttct ccacgtactc gggcaggatg aaatcgccgt ggcgcctgag accgcctccc
     4141 ttgcggaagt cgaagtagaa ccagccattc tcgtccctgt gacacatgtc cccggtcctc
     4201 aagaccccac ccctggtctt ttcctccgaa gccttcttct tgcccaggta ctcgacctcc
     4261 gcttttccct gcttggggac gacgatgagc tcgccgatct ccccgggggc acactcggtg
     4321 tcgtcctccc ggaccacctt catctccatg atcccctcga tgggcttccc gaaagacccg
     4381 atgggcccca caccaggcgg gttgtgggcg aatcccccct cgaccgcgcc gtaccactcg
     4441 tggatcttca cgttgaaacg cttctcgaaa tcctcccaga tgctcctggg ggtccctgcg
     4501 ctgagaacga ccctcacggg gttgtccgcg tcgtccggcc tcctgggctc gctgtagagc
     4561 cccatcat
//
LOCUS       DAOSCR010000155         4566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_11956_length_4566_cov_3.416316, whole genome shotgun sequence.
ACCESSION   DAOSCR010000155 DAOSCR010000000
VERSION     DAOSCR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11956_length_4566_cov_3.416316"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLT69_05145"
     CDS             <1..365
                     /locus_tag="PLT69_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ13859.1"
                     /translation="DVLAVPNLLGDFISDALAAQVGGLGMAPGANIGIPYAVFEATHG
                     TAPKYAGQDKINPGSLILSGAMMFDYLGWTEAGALIRSGLERTIEDKLVTYDLERQME
                     GATLVSCSGFASAIVERM"
     gene            complement(404..616)
                     /locus_tag="PLT69_05150"
     CDS             complement(404..616)
                     /locus_tag="PLT69_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13860.1"
                     /translation="MDTEGFKANSKLIGIAVACALGFIFILQNFEQTSFQFLFWTLLS
                     APKWIVVGSTFAIGLAFGYLLGKKKA"
     gene            860..2569
                     /locus_tag="PLT69_05155"
     CDS             860..2569
                     /locus_tag="PLT69_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ13861.1"
                     /translation="MINEVIVVSRDLKVVAASDGAASILGTAKVSEGERIDARHPLLA
                     PLFEGRHEVVLDGAKARLAGTGETYVIVLSAPPGVARGNSTASLDAGLLEKIVEDAPI
                     GIIIADESGRIEYLNRKQEQNSRRRREDLYHRDLRDVYRRSYEHPEVVRMYENLRSGA
                     EEDQKAIIDHYNPQFYKRDMTIKFLGKRLKDYGKVAIFVEIEEELYRQKRKAEKAGEE
                     LRLSRTYLTRLLDASPNMVVSIDKNRTILSFNKTAERLLGCMAEEVYGTKVDRFFPKE
                     ELPKLFLAVDARIPWFGTTHLIHADGTGFPVELSSTKIVDEITGKEIATLLIAVDIRE
                     RNRLRKNLIQSQKMTFIGELVSGLAHQLNNPLVGVVNIAEVLMHAMERGDPRAEYVRM
                     IKDAGEHCREVIARLLRFSRRHDTTTHVDVDVVAVMGASIELLSRHPRFGKIKVERDL
                     GVVPCVRGDPVLLQQAFMNILMNSAEAVGDDGVIRVGCSLQNGTVPQVEVTIADNGRG
                     IPEEHIPRIFEPFYSTKADDEGTGIGLSLAYWIVQDHGGRIDVESTPGKGTCFVVMLP
                     AKE"
     gene            2577..3983
                     /locus_tag="PLT69_05160"
     CDS             2577..3983
                     /locus_tag="PLT69_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ13862.1"
                     /translation="MEGNKPGILVVDDEEYTRAFFKGILPPDRYRLALAKDGAEALRL
                     WSEGGVDLVIMDIRMPVLDGMEALRRIRATDQDTMVIMISAYGDMDSVIEAMRLGAND
                     FFTKPFASIDKILLDIRNCLERRSLMRENATLRRQVEAELTRHDLVFVSKAMERIVAM
                     AARAAELDMPVLLLGESGTGKEVLARLIHARSPRSKAQFFAVNCGALTDTLLESTLFG
                     YEKGAFTGAAATTPGYFEAARGGTIFLDEIGETPPAFQVKLLRVLQEGEIMRVGGTKP
                     IRVDFRLVSATNTDLADLVARGAFRRDLYYRINVVRIEIPPLRERPEDVPVLLEHFME
                     RVCRRNNLKPRRFSREALDVLVRQGWEGNVRELANLVERLMVSTGRGPVSVEDLPPEY
                     REPSPCASRSGLELDYQKARDAFERAYFSRLVEAAGGDLRRASELSGLDLSTLYRKKK
                     RVSPLDMHDRKGRLHSRE"
     gene            4235..>4566
                     /locus_tag="PLT69_05165"
     CDS             4235..>4566
                     /locus_tag="PLT69_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOJ13863.1"
                     /translation="MPVFKNTEHLYETLGGFWKALYERQDFSQAVKSAGIQIKFEITD
                     PQAVIWVGPDGVEFGEQNLKPDVTMQLAGDTCHAFWKKEVSLPVALAKRQIKSKGNIA
                     KVMKLLPLV"
BASE COUNT          930 a         1344 c         1529 g          763 t
ORIGIN      
        1 acgatgtcct cgccgtgccg aacctcctcg gcgacttcat ctcggacgcg ctcgccgccc
       61 aggtgggggg gctcggcatg gcgccgggcg cgaacatcgg gatcccgtac gcggtcttcg
      121 aggcgacaca cgggaccgcg cccaagtacg ccggccagga caagatcaac ccgggctcgc
      181 tcatcctttc gggcgcgatg atgttcgact acctgggatg gacggaggcg ggggcgttga
      241 tcaggtccgg tctcgagagg acgatcgagg acaagcttgt cacctatgac ctcgagcggc
      301 agatggaggg ggcgacgctc gtgtcctgct cggggttcgc ctccgcgatc gtcgagagga
      361 tgtgacggta cgggccttgg gcgtttcctg agcgcgccgt ctctcaggcc ttcttcttgc
      421 cgaggaggta accgaaggcg aggccgatcg cgaaggtgga tccgaccacg atccacttgg
      481 gcgcggagag cagggtccag aacaggaact ggaacgaggt ctgctcgaag ttctggagga
      541 tgaagatgaa gccgagcgcg caggccacgg caatgccgat gagcttggag ttcgccttga
      601 acccttcggt atccatgccc agcccctctt ggcgatgatc ctgaactatg gcatgcgccc
      661 gggtgcgtgt caacatgcat tcgccccgct caagggccgc tggcccgtct tgtggaagaa
      721 caccccgtgc ggggtctcaa caagacaggg tccctgcccg aggagacgca tgccggcagg
      781 gggtgcccgg agaccgtgag gtgtatgccc gcggccggcc cttttgccag gcccacggtg
      841 cgtgtggtat catggaacga tgatcaacga ggtgatcgtc gtatcgaggg acttgaaagt
      901 ggtggcggcc tccgacggcg ctgcgtccat actcggcacg gcgaaggtgt ccgagggcga
      961 gaggatcgac gctcgtcacc ccctcctcgc acccctcttc gaaggcaggc atgaggtcgt
     1021 tctcgacggc gcaaaggcga ggcttgccgg tacgggggag acgtacgtga tcgtgctttc
     1081 ggcccctccc ggggttgcaa gggggaactc gacggcttcg ctggacgccg ggcttctcga
     1141 gaagatcgtc gaggatgcgc ccataggcat catcatcgcg gacgaaagcg gcaggatcga
     1201 gtacctgaac aggaaacagg aacagaacag ccggaggagg cgcgaggacc tctaccaccg
     1261 ggacctcagg gacgtctacc gcaggtcata cgaacacccg gaggtcgtga ggatgtacga
     1321 gaacctcagg tccggggccg aggaggacca gaaggcgatc atcgatcact acaaccccca
     1381 gttctacaag cgggacatga ccatcaagtt cctcggcaag aggctcaagg attacggcaa
     1441 ggtggcgatc ttcgtcgaga tagaggagga actctacagg cagaagcgca aggctgagaa
     1501 ggccggcgag gaactgaggc tttcgaggac gtacctgacg agactcttgg acgcctcccc
     1561 gaacatggtc gtctccatcg acaagaacag gaccatcctc agcttcaaca agacggcgga
     1621 acgacttctg ggctgtatgg ctgaggaggt gtacggcacg aaggtggacc ggttctttcc
     1681 caaagaagaa ctgccgaagc tgttcctcgc cgtggatgcg cgcattccct ggttcgggac
     1741 cacgcacctc attcacgcgg acggaaccgg gttccctgtc gagctctcct ccaccaagat
     1801 cgtcgacgag atcacgggca aggagatagc gacgctgctc atcgcggtgg acatcaggga
     1861 gcgaaacagg ctcaggaaga acctcatcca gtcgcagaag atgaccttca taggcgagct
     1921 cgtcagcggg ctcgcgcacc agctcaacaa ccccctcgtg ggcgtggtga acatcgccga
     1981 ggtgctcatg cacgccatgg aacggggaga cccgcgggcc gagtatgtgc gcatgatcaa
     2041 ggacgcgggc gagcattgca gggaggtgat cgcccggctc ctgaggttct cgcgcaggca
     2101 cgacaccacc acccacgtcg acgtcgacgt ggtcgccgtg atgggcgcga gcatagagct
     2161 cctctcgaga cacccgcgtt tcggcaagat caaggtggaa cgagacctcg gcgtggtccc
     2221 ctgtgtcagg ggggacccgg tcctcctcca gcaggccttc atgaacatcc tcatgaacag
     2281 cgccgaggcg gtgggcgacg acggggtcat ccgggtgggg tgctccctgc agaacggcac
     2341 cgtgcctcag gtggaggtga ccatagcgga caacggcagg gggatccccg aggaacacat
     2401 cccgaggatc ttcgagccgt tctacagcac caaggcggac gacgagggaa cgggcatagg
     2461 gctgagcctc gcgtactgga tcgtgcagga ccacggcggg aggatagacg tggagagcac
     2521 ccccgggaaa ggcacctgct tcgtcgtgat gctgccggca aaggagtgaa cggggcgtgg
     2581 aagggaacaa gcccggcata ctcgtcgtcg acgacgagga atacacgagg gccttcttca
     2641 agggcatcct gccgcctgac agataccgcc tggcgctcgc gaaagacggg gcggaagccc
     2701 tgcgactgtg gtcggaggga ggggtcgacc tcgtcatcat ggacatccgc atgcccgtcc
     2761 tggacgggat ggaggcgctg cgcaggataa gggccaccga tcaggacacc atggtcatca
     2821 tgatctccgc ctacggcgac atggactcgg tcatcgaggc catgcggctg ggcgcgaacg
     2881 atttcttcac caagccgttc gcgagcatcg acaagatcct cctcgatatc cggaactgcc
     2941 tcgagagacg ctcgctcatg agggagaacg ccacgctcag gaggcaggtg gaggcggagc
     3001 tgacgaggca cgacttggtg ttcgtctcca aggcgatgga gcggatagtg gcgatggcgg
     3061 cgagggcggc cgagctcgac atgccggtgc tcctgctcgg ggagtccgga acgggcaaag
     3121 aggtcctggc ccgcctgatc catgccagga gccccaggtc gaaggcgcag ttcttcgcgg
     3181 tgaactgcgg ggccctcacg gataccctct tggaatcgac gctcttcggg tacgagaagg
     3241 gggcgttcac gggcgccgcc gccacgaccc cgggatactt cgaggccgcc cgcggaggga
     3301 ccatctttct cgacgagata ggagagaccc ctccggcgtt ccaggtcaag ctcctgcgcg
     3361 tcctccagga gggcgagatc atgcgcgtgg gcgggacgaa gcccatacgc gtggacttcc
     3421 gcctcgtgtc ggccaccaac accgacctgg ccgatctcgt ggccaggggg gccttccgca
     3481 gggacctcta ctaccgcatc aacgtggtga ggatcgagat cccgcctttg agggaaaggc
     3541 ccgaggatgt cccggttctg ctcgagcact tcatggagcg ggtgtgccgg cgcaacaacc
     3601 tgaagcccag gcgcttttcg agggaggcgc tcgatgtgct cgtgagacag gggtgggagg
     3661 ggaacgtgag ggagctcgcg aacctcgtgg agcgcctcat ggtgtccacg ggcaggggtc
     3721 ccgtctccgt cgaagacctg cccccggagt accgggaacc ttccccctgc gcaagccgaa
     3781 gcgggctcga gctcgactac cagaaggcgc gtgacgcgtt cgagcgcgcc tacttctcgc
     3841 ggctggtcga agcggcgggg ggtgacctca ggagggcctc cgagctctcc gggctcgacc
     3901 tctccaccct ctaccgaaag aagaagaggg tctcgcccct ggatatgcac gatcgcaaag
     3961 gccgtttgca ctcgcgcgaa taacccatct ccctggaatc tttggtcaaa taagccggcg
     4021 cctgaatcct ttcttgcggt cctgcaacgc gtgtacgtgc aactgcctgt ccatgtgtgt
     4081 gcgtggagtg aaatatcctc aagaatcata atctagtatg catggggacg cgcggtggca
     4141 cgggtattgc tgttataatg tgagtcaata ttataatcta agatccgcgc ccgtctcccg
     4201 ttggggcggg catcaacgca aaagggaggt tgacatgccg gtcttcaaga acacggaaca
     4261 cctgtacgag acgctggggg gattctggaa ggccctgtac gaaaggcagg atttttcgca
     4321 ggccgtcaag tccgcgggga tacagatcaa gttcgagatc acggatcccc aggcggtcat
     4381 ctgggtcggc ccggacggtg tggagttcgg tgagcagaac ctcaagcccg acgtcaccat
     4441 gcagctcgcg ggcgacacct gccatgcctt ctggaagaag gaggtgagcc ttccggttgc
     4501 cctcgcaaag agacagataa agtccaaggg aaacatagcc aaggtcatga agctgctgcc
     4561 cttggt
//
LOCUS       DAOSCR010000156         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12010_length_4551_cov_3.699956, whole genome shotgun sequence.
ACCESSION   DAOSCR010000156 DAOSCR010000000
VERSION     DAOSCR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12010_length_4551_cov_3.699956"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /locus_tag="PLT69_05170"
     CDS             <1..1868
                     /locus_tag="PLT69_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ13864.1"
                     /translation="ALEAGVSVAAGYPGTPSSEIIENLSRVADRAGLHVRWAVNEKVA
                     AEVAAAASFAGLRSICTMKQNGMHVASDFLLHLTGTGVRAGMAVIVCEDPGALSSQNE
                     ADARFFARMLEVPLLEPGDFQEAKDMTRWALELSEELKTFVILRSVTRMSHASGTVTL
                     GALSGNGKKAYFECTGSLLDFQRGPMITGPGAVTYARTVQQGRLRQASRMFDQSPFNS
                     YTGSLEPRLLVVSSSACALYAREAIEILGAWDRVGLLKLGTTWPLPEQLVLRHLAGAG
                     EVLVVEEPQDFLEDAVKVLAAEQASIAGAKVFYGKRSGHVPPAGELNCDVVIGALARI
                     LGVNYEPAPKDYADRALAATFTAPGRELTFCAGCPHRASFYCLHNALALKGKNGFVCG
                     DVGCYTMGILPAGFEVVKTAFCMGSGIGLASGFGKLGAFGFDQPVVAVCGDSTFFHAA
                     MPALVDAVHHEADVMLVVLDNSGTAMTGFQPHPGIGEDASGKRARPMDIEAICRAMGA
                     KVVVCDPFEVEGTERTLLSMLEESGPRVAILRQECALSPHKRAGKRFKIRVDEAACIG
                     SRCGCNRMCTRVFRCPGLFWDARAKVARIDEVICTGCGVCASVCPSGAIRKEEAG"
     gene            1872..2513
                     /locus_tag="PLT69_05175"
     CDS             1872..2513
                     /locus_tag="PLT69_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOJ13865.1"
                     /translation="MAGRTLSRDPFSVIVTGVGGQGNVTASRLIASMLVAKGYVVTVG
                     ETFGASQRGGSVMSHVRISACHTYSPQVPKCRADVVVSLEPTETIRVLASYGHPETRC
                     VVNDRPVYPVRVIAGEVEYPGIEEIDRTIAGLCRAHVMIGATSEALRLGNPVLQNVIM
                     IGALQATGLVPFTRADFEGLVSAEMAPATAELNLEAFDAGVRLAEQAMGGKAW"
     gene            2665..3795
                     /locus_tag="PLT69_05180"
     CDS             2665..3795
                     /locus_tag="PLT69_05180"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOJ13866.1"
                     /translation="MQRGQRELDRLVVDAKLCTGCGSCVGLCPYRRFYRDRIVTLFPC
                     DREEGRCFDFCPRTPADLDGIRKALFEPADTTPELGAVKAIYLARATREKVRSRAQHG
                     GTVSTLLSLALKEGLIDAAVVTDRKDAMDPFGVVVTDPERVVSHAGSSFVVSPSVAAF
                     NRAVRDGFANLGAVALPCQALAYAKMRVLPEDENNPPAQRPSLVIGLFCSWAFSRDRF
                     SELARKSVDPYLVRGMDIPPKQYGVLQLFTGERTVDIGLEEAKRCIRGGCHYCFDLTA
                     EFSDISVGSALLEEDWESARGWNTVIVRTRRARELLDLARKRRLLECREIPSQVVSEL
                     KKGSLEKRARARRNLVLKSGREGDLVYLDPNDPMFSALQQGA"
     gene            3792..4541
                     /locus_tag="PLT69_05185"
     CDS             3792..4541
                     /locus_tag="PLT69_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOJ13867.1"
                     /translation="MRTMEVSLFIPCTVDVLLPEIGVATCRVLERLGVKTVYHESQTC
                     CGQVFLNAGHAQKARRAACRFIEIFSKDDFVVSPSGSCVHMVRSCYPDLFPAGSLWRA
                     RALDLAPRVFELSQFLVDVMGVTDPGGSFDATVTYHDSCHVLRGLGVSRQARMLVDAA
                     AGPRRVEMPGADSCCGFGGEFSVRFPEISVAILKDKVQSFLDSRADVLVLNEPGCLLN
                     IGGYLHRHHPEKRVMHLAVFLDEAMERGSGR"
BASE COUNT          763 a         1400 c         1625 g          763 t
ORIGIN      
        1 gggcgctgga ggccggggtc tcggtggcgg ccggttaccc ggggacgcct tcgtccgaga
       61 tcatcgagaa cctctcgcgc gtggctgacc gggccgggct ccacgtgagg tgggcggtga
      121 acgagaaggt ggccgccgaa gtggccgcag cggcgtcgtt cgcggggctc aggtctatct
      181 gcaccatgaa gcagaacggc atgcacgtgg catcggactt cctcctccac ctcacgggca
      241 ccggggtgag ggccggcatg gccgtcatcg tgtgcgagga cccaggggcg ctctcgagcc
      301 agaacgaggc ggatgccagg ttcttcgcga ggatgctcga ggtccccctg ctggagcccg
      361 gggatttcca ggaggccaag gacatgacgc gctgggcgct cgagctctcg gaggaactca
      421 agaccttcgt catcctgagg agcgtcacgc gcatgtccca cgcgagcggc accgtgaccc
      481 tcggggcgct ttccggcaat ggcaagaagg cgtacttcga gtgcacgggc tcgctgctcg
      541 acttccagag ggggcccatg ataaccggcc ccggcgcggt gacctatgcc aggaccgtgc
      601 agcaggggcg gctgaggcag gcatcccgga tgttcgacca gtccccgttc aacagctaca
      661 cgggatcctt agagccccgc ctcctcgtcg tgtcgtcgag cgcctgcgcc ctgtacgcca
      721 gggaggcgat cgagatcctc ggcgcctggg acagggtggg gttgctcaag ctcgggacca
      781 cgtggcccct gcccgagcag ctcgtcctga ggcacttggc tggggcgggc gaggtgctcg
      841 tggtcgagga gccgcaggac ttcctggagg acgccgtgaa ggtgctcgca gctgagcagg
      901 cttccatagc gggcgcgaag gtgttctacg gcaagcgctc cggccacgtg cccccggccg
      961 gtgagctcaa ctgcgacgtg gtgataggcg ccttggcgcg catcctgggg gtgaactacg
     1021 agccggcacc caaggactac gcggacaggg ccctcgccgc cacgttcacc gcacccggca
     1081 gggagctgac cttctgcgcc ggctgcccgc acagggcgtc gttctactgc ctgcacaacg
     1141 cgctcgcgct caaggggaag aacgggttcg tctgcgggga cgtgggctgc tacaccatgg
     1201 ggattctgcc ggcaggcttc gaggtcgtca agacggcctt ctgcatgggg tcgggcatcg
     1261 gcctcgcatc aggtttcggt aagctcggcg ccttcggttt cgaccagccg gtggtggccg
     1321 tgtgcggcga ctcgaccttc tttcacgcgg cgatgccagc gctcgtggac gcggtacacc
     1381 acgaggctga cgtgatgctc gtggtgctcg acaacagcgg cacggcgatg acgggtttcc
     1441 agccccaccc gggcatcggt gaggacgcct cgggcaagag ggctcgaccc atggacatcg
     1501 aggccatatg ccgcgccatg ggcgcgaagg tcgtggtatg cgaccccttc gaggtggagg
     1561 ggaccgagcg caccctcctg tccatgctcg aggaatcggg tccccgggtg gccatcctca
     1621 ggcaggagtg cgccctcagc ccccacaagc gcgccggcaa aaggttcaag atccgcgtgg
     1681 acgaggccgc ctgcataggt tcgaggtgcg gctgcaacag gatgtgcacc cgcgtcttca
     1741 ggtgcccggg cctcttctgg gacgcccggg cgaaggtcgc ccgcatcgac gaggttatct
     1801 gcaccgggtg cggggtatgc gcgagcgtct gcccctcggg ggcgatcagg aaagaggagg
     1861 ctgggtgaac gatggccggt aggacattga gccgcgatcc gttcagcgtc atagtcaccg
     1921 gtgtgggggg gcagggcaac gtgaccgcat cgaggctcat cgcctccatg ctggtcgcca
     1981 aggggtacgt cgtgaccgtc ggtgagacct tcggcgcctc ccagaggggg ggctcggtca
     2041 tgagccacgt caggatctcg gcctgtcaca cctacagtcc ccaggtgccg aagtgcaggg
     2101 ccgacgtggt ggtctccctg gaaccgaccg agaccataag ggtcctcgcc tcctacgggc
     2161 acccagagac ccgctgcgtg gtgaacgacc ggcctgtgta tcccgtccgc gtgatcgcag
     2221 gcgaggtcga gtaccccggg atcgaggaga tagaccgcac gatagcgggt ctctgcaggg
     2281 ctcacgtgat gatcggagcg acgtccgagg ccctgaggct cgggaacccc gtgctccaga
     2341 acgtaatcat gatcggggcc ctccaggcca cggggctcgt gcctttcacg agggcggatt
     2401 tcgagggcct ggtctcggcg gagatggcac cggccacggc ggaactcaac ctcgaggcct
     2461 ttgatgcagg ggtccgtctc gccgaacagg cgatgggggg caaggcatgg tgatgacggg
     2521 aaggaccggg acccttcccc gatgagacga ccagcggccg ggagggtttc gttcaagggt
     2581 acagagcgtc gagcccctgc cgggttgcgg cgcggtcgtg ctggggaatg acggcttcag
     2641 gggctccttg ccatggagga gagggtgcag agaggacaga gggaattgga caggctggtg
     2701 gtggatgcga agctctgcac gggctgcggt tcgtgcgtgg ggctctgccc gtacaggagg
     2761 ttttacaggg acaggatcgt gaccctgttt ccctgcgaca gagaagaagg gaggtgcttc
     2821 gacttctgcc cgaggacccc ggccgacctc gacggcatca ggaaggcgct gttcgaaccg
     2881 gctgacacta cgcccgagct gggggccgtc aaagcgatct accttgccag ggctacgcgg
     2941 gagaaggtga ggagccgagc gcaacacggg ggcacggtct cgacgctctt gtccctcgcg
     3001 ctcaaggagg gcctgatcga tgccgcggtc gtgaccgaca ggaaggatgc catggacccg
     3061 ttcggggtcg tggtgaccga tcccgaacgg gtggtctcgc acgccgggag ttccttcgtg
     3121 gtctccccga gcgttgcggc cttcaacagg gctgtcaggg acggtttcgc gaacctgggc
     3181 gcggtggccc ttccctgcca ggccctcgcg tacgcgaaga tgcgcgttct ccccgaagac
     3241 gagaacaacc ctcctgcaca aaggccgtcg ctcgtgatcg ggctgttctg cagctgggcc
     3301 ttcagccgcg acaggttctc ggagctggct cgcaagagtg tcgatccgta cctcgttcgg
     3361 ggcatggaca tcccgcccaa gcagtacggg gtgctccagc tcttcaccgg cgagaggacc
     3421 gttgacatag ggctcgaaga ggcgaagcgc tgcatacggg gggggtgcca ctactgtttc
     3481 gatctcaccg ccgagttcag cgacatatcc gtgggaagcg cgctgctcga ggaggactgg
     3541 gagtcggcga ggggctggaa caccgtcatc gtgcgcactc ggcgggcgag ggagcttttg
     3601 gatctcgccc ggaagaggcg cctgctggaa tgcagggaga tcccgtccca ggtggtctcc
     3661 gagctcaaga aggggtccct ggagaagagg gcgcgggcga ggagaaacct cgtgctcaag
     3721 tccggccggg aaggagacct cgtgtacctc gacccgaacg acccgatgtt ttccgccttg
     3781 cagcaggggg catgaggacc atggaggtct ccctcttcat cccgtgcacc gtcgatgtcc
     3841 ttctcccgga gataggcgtc gcaacctgca gggtcctcga gagactcggc gtgaaaacgg
     3901 tgtaccacga gagccagacg tgctgcgggc aggtcttcct caacgccggg cacgcgcaga
     3961 aggcgagacg ggccgcgtgc cgcttcatcg agatcttctc caaggacgac ttcgtcgtga
     4021 gccccagcgg gtcgtgcgtg cacatggtca ggagctgcta tcccgacctc ttcccggcgg
     4081 gcagcctctg gcgggcccga gccctcgatc tcgcacccag ggtgttcgag ctgagccagt
     4141 tcctggtgga tgtcatgggt gtgaccgatc ccggagggtc gttcgacgcc acggtgacct
     4201 accacgattc gtgccacgtg ctcagggggc tcggcgtgtc gcgccaggcc aggatgctcg
     4261 ttgacgccgc ggcaggtccc cgcagggtgg agatgcccgg ggccgactcc tgctgcgggt
     4321 tcggggggga attctccgtg aggttcccgg agatatccgt ggcgatactc aaggacaagg
     4381 tccagtcctt cctggacagc cgcgcggatg tgctcgtgct gaacgagccc ggatgcctcc
     4441 tgaacatcgg cggctacctc cacaggcacc acccggaaaa gagggtcatg cacctggcgg
     4501 tcttcctcga cgaggccatg gaaaggggga gtggccgatg agctctcctt c
//
LOCUS       DAOSCR010000157         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12047_length_4537_cov_3.369032, whole genome shotgun sequence.
ACCESSION   DAOSCR010000157 DAOSCR010000000
VERSION     DAOSCR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12047_length_4537_cov_3.369032"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLT69_05190"
     CDS             <1..324
                     /locus_tag="PLT69_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOJ13868.1"
                     /translation="HAPASPAYERLAMRLGVIFALEEYAPGRLPPFACRILLDASRGR
                     GRAGRWEDLGVPYILAGGLTPDNVGEAVRRARPFGVDVSSGVESAPGIKDPGKIERFI
                     REARS"
     gene            321..1475
                     /gene="trpB"
                     /locus_tag="PLT69_05195"
     CDS             321..1475
                     /gene="trpB"
                     /locus_tag="PLT69_05195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOJ13869.1"
                     /translation="MKGYFGEFGGQYVPETLLPALEELEEAMLQAFADDAFSAELQAY
                     LSDYVGRPTPLTHALRLSAHYGCDVWLKREDLTHTGSHKINNAIGQILLARRMGKRRV
                     IAETGAGQHGVATATVCALFGMECRVYMGSTDMARQRVNVERMRLLGAEVSPVESGGA
                     TLKEATSEAIRDWIANVDTTYYLIGSAIGPHPYPTLVRELQAVIGREAKAQFETLAGG
                     LPDAVVACVGGGSNAIGIFSAFLDEASVELFGVEAYGEGNGRHGASINFGYPGVLHGT
                     RSMLLQDKDGQVCETHSVAAGLDYPGVGPEHSHLAATKRASYRLVTDEQALAAFRLLG
                     RLEGIVPALESAHAIAFLEQYSRQNSGRRVLVNLSGRGDKDVHTVFGGAA"
     gene            1472..2236
                     /gene="trpA"
                     /locus_tag="PLT69_05200"
     CDS             1472..2236
                     /gene="trpA"
                     /locus_tag="PLT69_05200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOJ13870.1"
                     /translation="MIEERIRSTKRAVIPYVTAGLPDLDTTGDILDVLAAGGAAAIEI
                     GIPFSDPVADGPVLQRASYLALKAGFRMCKLMERLGSWSARVSVPLVIMTYINPLMRK
                     GLEDFLTALRDAGVQGVIIPDLPVDAGGIFDHCSRIGLDLVRLVAPTTKRARAREILS
                     RCSGFVYAVSVKGVTGQRTSLPEGIDAQVASIRELTTLPVCVGFGVSRAGQVDELLGF
                     ADGVIVGSHLMQEVMSGTDPVERARDAFARLMNGSR"
     gene            complement(2217..3371)
                     /locus_tag="PLT69_05205"
     CDS             complement(2217..3371)
                     /locus_tag="PLT69_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ13871.1"
                     /translation="MAEKTRPPAKILHIISTLDTGGAEQNLFRLVSTLDRGSFTSEVV
                     CMRSPGPVAARITASGVPVRPLHMRPGVPSPLGVMRLWSTAGRFRPDIVQCWMYHANL
                     MGLCLFMPGRTVWNIRCSDMDLARYGGAYRFAVRAGAALSGIPRAVIVNSDAGRKAHE
                     AIGYRPRRWVTIRNGFDTELFRPDASARSAVRAELGIDEHALVIGLVGRLDPMKDHAT
                     FFQAAAMFLETYKDATFVLAGRAVTADNPALTALIPASAPRAAFRLLGERDDVQRVLA
                     CMDIAAGSSVYGEGFPNALAEAMACGVPCVATDVGDTSLLVGDTGIVVERGSAQALCR
                     GLETLARMGPDQRRRLGARARTRVETLFTQKNTTAAYEDLYRKILTSSRS"
     gene            complement(3364..>4537)
                     /locus_tag="PLT69_05210"
     CDS             complement(3364..>4537)
                     /locus_tag="PLT69_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ13872.1"
                     /translation="ALLKHAVCQQMVADVPLGAFLSGGIDSSTVVALMQAQSSRPVKT
                     FTIGFREENYDESGWARAVASRLGTEHTEETVTPEHAREILPLWPWIYDEPFGDISGI
                     PTTIVSRITRRHVKVSLSADGGDELFCGYHRYWVMARLDGLLSRLPDAVPRMAGRALA
                     ALGTDVAARMALALPGLRLPALRDRMRKFQAVLENWEGGAGPAYPYAVAYWLPSEVDR
                     LVGGYENCRPLPGESDWGLLKSMMLWDLVHYLPEDILAKVDRATMSVGLEGRDPFLDH
                     RVVEYALGLPLDLTYRGGTMKYILKKILARHLPKRLFERPKQGFAVPVHSWLHDDLLH
                     LVDENLNPDTLGAQTEIDPEPVLETVALFKGGRGSMAVDRVWLALVYMMWRRTYLG"
BASE COUNT          735 a         1504 c         1581 g          717 t
ORIGIN      
        1 cacgcgcccg cctcgcccgc ctacgagagg ctcgcaatgc gcctcggcgt gatcttcgcg
       61 ctcgaggagt acgcgccggg gaggctcccg ccctttgcgt gccgcatcct cctggacgcg
      121 agcaggggca ggggcagggc cgggaggtgg gaggatctcg gcgtccccta catcctggcg
      181 ggaggcctca ccccggataa cgtgggcgag gccgtgaggc gcgcccgccc cttcggggtg
      241 gatgtgtctt cgggagtgga atcggccccc gggatcaagg acccggggaa gatcgagagg
      301 ttcatcaggg aggcgagatc atgaagggat atttcggcga gttcggagga cagtacgtcc
      361 cggagacgct cctcccggcg ctggaggagc tcgaggaggc catgctgcag gcgttcgctg
      421 acgacgcgtt cagtgcggag cttcaggcct acctttccga ctacgtgggg aggcccaccc
      481 cgctcacgca cgctctcagg ctctcagccc actacgggtg cgacgtgtgg ctcaagaggg
      541 aggacctgac gcacacgggt tcccacaaga tcaacaacgc catcggccag atcctgctcg
      601 cgaggcgcat ggggaagagg cgcgtcatcg ccgagaccgg cgcgggccag catggtgtgg
      661 cgacggcaac ggtgtgcgcc ctgttcggca tggagtgccg cgtgtacatg ggcagcacgg
      721 acatggccag gcagcgcgtg aacgtggagc gcatgaggct cttgggcgcc gaggtctcgc
      781 cggtggaatc gggaggagcc accctgaaag aagccacgag cgaggcgatc cgtgactgga
      841 tcgcgaacgt ggacacgacc tactacctca tcgggtcggc gatcggaccg cacccgtacc
      901 cgacgctcgt cagggagctc caggcggtga tcgggcgtga ggccaaggcc cagttcgaga
      961 ccttggcagg cgggctgccg gacgcggtgg tggcgtgcgt tggcgggggc agcaacgcca
     1021 tcgggatctt cagcgccttc ctcgatgagg cctcggtgga actcttcggg gtggaggcat
     1081 acggcgaggg caacggcagg cacggcgcga gcatcaactt cggttacccc ggcgtgctcc
     1141 atggtacccg ctcgatgctc ctccaggaca aggacggcca ggtgtgcgaa acccactcgg
     1201 tggcagccgg gctcgactac cccggagtcg gaccggaaca cagccacctt gcggccacga
     1261 agagggcgag ctaccggctc gtcaccgacg agcaggccct tgccgccttc aggctcctgg
     1321 gcaggctgga gggcatcgtc cccgcgctcg agagcgcgca cgcgatcgcg ttcctcgagc
     1381 agtacagcag gcagaactcc ggcaggcgcg tcctcgtcaa cctctccggc aggggtgaca
     1441 aggacgttca caccgtgttc gggggtgcgg catgatcgag gagaggatcc gctcgacgaa
     1501 gagggccgtc atcccgtacg tgacggcagg gctcccggac ctggacacga ccggggacat
     1561 actggacgtt cttgccgccg ggggggcggc ggccatcgag atcggcatcc cgttttccga
     1621 tcccgtggcg gacggacccg tcctccagag ggcctcctac ctcgccctga aggccggctt
     1681 ccggatgtgc aagctcatgg agcgtctggg ctcgtggagc gccagggtct ccgtccctct
     1741 cgtgatcatg acctatatca accccctcat gcgaaaaggc ctcgaggact tcctcaccgc
     1801 gctcagggac gcgggggttc agggcgtcat catacccgac ctgcccgttg acgccggggg
     1861 tatcttcgat cactgctcga gaatcgggct cgacctcgtc aggctcgtag cgccaaccac
     1921 gaagagggcc cgcgcgcggg agatcctctc ccggtgctcg gggttcgtct acgccgtctc
     1981 ggtcaagggg gtcacgggac agaggacgag cctgcccgag gggatcgacg cccaggtggc
     2041 gtcgatcagg gaactcacca cgctgcccgt gtgcgtgggg ttcggggtct cgagggcggg
     2101 ccaggtggac gagttgctcg ggttcgcaga cggcgtgatc gtgggtagcc acctcatgca
     2161 ggaggtcatg agcgggaccg acccggtcga gcgtgcgcgc gacgcgttcg cgcggctcat
     2221 gaacggctcg aggtgaggat tttgcggtac aggtcctcgt aggcggccgt ggtgttcttc
     2281 tgggtgaaga gggtctccac cctcgtgcgg gcccgcgcgc ccaatcggcg cctctggtcg
     2341 ggacccatgc gggcgagggt ctccagcccc cggcagaggg cttgagcgga acccctctcc
     2401 acgacgatcc ccgtgtcacc tacgagcagg gaggtgtcgc cgacgtctgt cgccacgcac
     2461 ggcacgccgc acgccatggc ctcggcgagg gcgttgggga atccctcgcc gtagacggac
     2521 gagccggctg cgatatccat gcacgccagc accctctgca cgtcgtcccg ctcgccgagc
     2581 agcctgaagg cggccctggg ggcggaagcg gggatgaggg cggtgagggc tgggttgtcc
     2641 gccgtcacgg ccctgcctgc gaggacgaat gtcgcgtcct tgtatgtctc aaggaacatc
     2701 gccgcggcct ggaagaaggt cgcgtggtcc ttcatggggt ccaggcggcc cacgaggccg
     2761 atcaccaggg catgctcgtc gatgcccagc tctgcgcgga ccgccgaccg ggcggaggcg
     2821 tcaggcctga agagttccgt gtcgaacccg ttgcggatcg taacccagcg tctgggcctg
     2881 tacccgatcg cctcgtgcgc cttcctgccc gcatccgagt tgacgatgac ggcccggggg
     2941 atccccgaca gtgccgcccc cgccctgacc gcgaaccggt atgcgccgcc gtacctggcg
     3001 aggtccatgt cggagcagcg gatgttccac acggtcctgc caggcatgaa caggcagagg
     3061 cccatgaggt tggcgtggta catccagcac tggacgatgt cgggcctgaa acgcccggct
     3121 gtggaccaca gccgcatgac ccccagcggg ctcgggacac ccggcctcat gtggagcggg
     3181 cgtaccggca cgcccgaggc cgtgatgcgg gcggcgacgg gcccggggct ccgcatgcac
     3241 accacctcgc tcgtgaagga gcccctgtcc agggtgctca cgagcctgaa caggttctgt
     3301 tcggcccccc ccgtgtccag ggtggagatg atgtggagga tcttggccgg aggccgggtt
     3361 ttctcagcca aggtaggtcc tcctccacat catgtacacg agggccagcc acaccctgtc
     3421 aaccgccatg gagccgcgcc cgcccttgaa cagggccacg gtctcgagca cgggctcggg
     3481 gtcgatctcc gtctgggcgc ccaaggtgtc gggattgagg ttctcgtcca ccaggtgcag
     3541 gaggtcgtcg tgcagccacg agtggacggg caccgcgaac ccctgcttgg gcctctcgaa
     3601 gagccgcttc gggaggtggc gggccaggat cttcttcagg atgtacttca tcgtgccccc
     3661 gcggtaggtg agatcgagcg gcagcccgag ggcgtactcc accacccggt gatccaggaa
     3721 ggggtccctc ccctcgagcc ccacggacat ggtggcgcgg tccaccttgg cgaggatgtc
     3781 ctcgggcagg tagtggacga ggtcccagag catcatggac ttcaagaggc cccagtccga
     3841 ctcccccggc agcggcctgc agttctcgta tccccccacg agccggtcca cctcggacgg
     3901 cagccagtac gcgactgcgt acgggtaggc cgggccggcc ccgccctccc agttctcgag
     3961 caccgcctgg aacttcctca tgcggtccct cagggcgggc agcctgagac ccggcagggc
     4021 gagcgccatc cgcgccgcca cgtcggttcc gagggctgcc aacgccctgc cggccatgcg
     4081 cggcacggcg tcgggcagcc tcgagaggag accgtcgagc ctcgccatca cccagtacct
     4141 gtggtacccg cagaacagtt cgtctccgcc gtccgcggaa aggctcacct tcacgtgcct
     4201 gcgcgtgatg cgggagacta tggtggtggg gatgccggag atgtcgccga agggctcgtc
     4261 gtagatccac ggccacaagg ggagtatttc ccgcgcgtgc tcgggcgtca cggtctcctc
     4321 ggtgtgctcg gtcccgagac gacttgccac cgccctggcc catcccgact cgtcgtagtt
     4381 ttcctccctg aagccgatgg tgaaggtctt gaccggccgg ctggactgcg cctgcatcaa
     4441 cgccaccacg gtgctggagt cgatgccacc cgacaaaaag gcacccagcg gcacgtcggc
     4501 caccatctgc tggcacacgg cgtgtttcag cagcgcc
//
LOCUS       DAOSCR010000158         4532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12063_length_4532_cov_2.863748, whole genome shotgun sequence.
ACCESSION   DAOSCR010000158 DAOSCR010000000
VERSION     DAOSCR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_4532_cov_2.863748"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PLT69_05215"
     CDS             complement(<1..1130)
                     /locus_tag="PLT69_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ13873.1"
                     /translation="MGTDKKGNSRKVWIRISPWVIMGSLVVMAPIFIFITMESIRIQR
                     RSIELMLSEKGGALIRAFEAGTRTGMMWGDWSGSRVQQLIRETADLPDILYILITDEK
                     GTIIAHNQPLNIGLKYGPDLPKVIDRTLRWRIVMTPNGTRVFEVYRRFHPSRPGMFPR
                     PGHPMHYRYQDWFSPHMLPAPSAPPVQVIYVGLDMEPIERVVNESIKQKVILAVFLLM
                     YGLLGIVAVVIVQNYLSTRASLSRVRAFSDTLVENMPMGLVVIGETGTLLSLNDVSER
                     LLGISGRDWADSKAKDILPREIVDMIVEVDDEHPLATKELELDAGGRNLSLEATAGSL
                     RDEEGRLLGHLVLLRDVTEIEHLKREMERKERLASLGSLAAGV"
     gene            1694..2194
                     /locus_tag="PLT69_05220"
     CDS             1694..2194
                     /locus_tag="PLT69_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOJ13874.1"
                     /translation="MKRTMIIVLTLSILTALAVPLMAWGPRWGYGPDGRGMMYDRDDR
                     GDRGGFCRGGMYFAPGAGDLTPEQRERVDKLEAELYKKLEPLAQQERIKVLELEAAIN
                     ADKPDEAKVLDLHKQIASIRDQIERARIRHQLEVKKVVPEARFRPVYMRGFMGGMGFG
                     RGCGRI"
     gene            2967..4004
                     /gene="purM"
                     /locus_tag="PLT69_05225"
     CDS             2967..4004
                     /gene="purM"
                     /locus_tag="PLT69_05225"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOJ13875.1"
                     /translation="MSLTYRDAGVDIDKQNQLVRAIKSSVRSTHGPEVIGGIGGFAGL
                     FKLDTGAYTDPILVSSTDGVGTKLKIAFMADNHSTVGIDLVAMVVNDLVVVGAQPLFF
                     LDYFATGALSLERAKAVISGITRGCAEAGCALIGGETAEMPGFYREDEYDLAGFGVGV
                     VDAEKIIDGSRIAAKDVIIGIHSSGLHSNGFSLVRKVLFERAKLRVDDYLEDIGRTLA
                     EELLTPTRIYVKSVLNVIRSFEIKGIVHITGGGFVDNIPRVLPSRSCARIRKGSWEVP
                     PIFRFIQEVGKIEDHEMYRTFNMGVGMILIVSPKDADDVMLRLKGLKETADIIGYIDA
                     RTKGMDPVIIS"
     gene            4001..>4532
                     /gene="purN"
                     /locus_tag="PLT69_05230"
     CDS             4001..>4532
                     /gene="purN"
                     /locus_tag="PLT69_05230"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOJ13876.1"
                     /translation="MIRIGVLVSGSGSNLQAIMDACASGLIDGTVAVVISNVPDAYAL
                     VRARKMSVPTVVVQHEAYPDRESFDEALAGKLEEYQVDLVTLAGFMRVLTPAFLRRFP
                     GRIMNIHPALLPSFPGLHVRQKALDYGVRFSGCTVHFVDEGVDTGPIIIQAVVPVYPD
                     DTEEELNERILRLEHRI"
BASE COUNT          901 a         1351 c         1436 g          844 t
ORIGIN      
        1 accccggcgg cgaggctccc cagggaggca aggcgttcct tgcgttccat ctcgcgcttg
       61 agatgttcta tctcggtgac gtcgcgcagg agcacgaggt gcccgaggag gcggccttcc
      121 tcgtccctga ggctgcccgc ggtcgcttca agcgagaggt tcctcccccc tgcgtcgagc
      181 tcgagctcct tggtggcgag cgggtgttcg tcgtccacct ccacgatcat gtcgacgatc
      241 tctcgaggga ggatgtcctt cgccttcgag tctgcccagt ctctgccgga gattccgagc
      301 agcctctccg agacgtcgtt cagggataac agggtccctg tctcgccgat cacgacgagc
      361 cccatgggca tgttctcgac cagcgtgtcg gagaaggccc tcaccctcga gagtgaggcg
      421 cgggtggaga ggtagttctg gacgatgacc acggcaacga tgcccaacag cccgtacatg
      481 aggaggaaca ccgcgaggat caccttctgc ttgatgctct cgttgaccac cctctcgatg
      541 ggttccatgt caaggcccac gtagatgacc tggaccggtg gggccgacgg tgccgggagc
      601 atgtgggggg agaaccagtc ttgataccgg tagtgcatgg gatgcccagg cctcgggaac
      661 atgcccggac gcgacgggtg aaaacgccgg tatacctcga acacccgggt gccgttcggt
      721 gtcatgacga tccgccacct gagggtcctg tcgatgacct tggggaggtc aggtccgtac
      781 ttcagaccga tgttgagggg ctggttgtgg gcgatgatcg tgcccttctc gtcggtgatg
      841 aggatgtaga ggatgtcggg gagatccgcg gtttccctga tgagctgttg caccctcgac
      901 ccgctccagt ccccccacat catccctgtc ctcgtccctg cctcgaaggc gcggatcagg
      961 gcacccccct tctcgctgag catcagctcg atactcctgc gctggatcct gatgctctcc
     1021 atggtgatga aaatgaagat cggcgccatc accacaagag accccatgat cacccacggg
     1081 gatatgcgga tccacacctt gcgagagttg cctttcttat cggtgcccat gtgtttcaga
     1141 aaactctggt tttcaacatc catgccatgt ccccaaggcg aagcgccgga tgctcggcgt
     1201 ccatgaccag gcccctcgtg ccgttcgtcg gagaggaggc gagaacgggt cgagacaggg
     1261 gctcacaggc gaccgggcac ccggggagag acgatgtcgc gaaccgaaaa ccatgtgtga
     1321 aaccagtgcg ttgtgaggcg ttgacggtgc atggcggcct cctcgggcat cgaggttcac
     1381 ctccaggcag actcctcgtc gtccatgccc gaaaccgagg gccgttcgtc cccccggcca
     1441 cactgcttcc gcgaggtacc ctgctcgagg tatgcaccat gtatagtttc tatacacgaa
     1501 gcttgggcga tgtgtataga aaatgtacag gagcatcgat gccggggatc atccatggcg
     1561 tgtagacggc gggcggcatt cgtaagggga tgatatcaca aggagaaaga gactcgtcga
     1621 tgcattggca cgggtgttgc tcaatgaggg gcaagctgag tgaaccgaca cactcgaaca
     1681 aggaggatca ccaatgaaac ggaccatgat aatcgtcttg acgctctcca tcttgaccgc
     1741 actggccgtg cccctcatgg cctggggtcc gaggtggggt tacgggcccg acgggagagg
     1801 catgatgtac gacagggacg accgtggaga cagaggcggt ttctgccgcg gcgggatgta
     1861 tttcgccccg ggcgcaggcg atctcacccc cgagcagcgg gagcgggtcg ataagctgga
     1921 agccgagctc tacaagaagc tggagccgct cgcgcagcag gagaggataa aggtcctcga
     1981 actcgaagcc gccatcaacg ccgacaagcc ggacgaggcg aaggtcctcg acctccacaa
     2041 gcagatcgcg tccatcaggg accagatcga gcgcgcccgg atacggcacc agctggaggt
     2101 caagaaggtg gtgcccgagg caaggttcag accggtctac atgcgcggct tcatgggcgg
     2161 catgggcttc ggccgcggct gcggacggat ctgacgccgg gaggggaccg ggcggggcga
     2221 aagcccgggg gacaccctca ggacagagga aacaaaagga cagaggaaac aagaaaaagg
     2281 ctcatggcta tgaagaaagc ccaggaacct tcttcataac cacacaactc ctatctacct
     2341 cctcttggga tggcggccaa tgcccatacc gccatccttt tttttcgact tcgatgcgac
     2401 cctgatcgct gttcatggaa ccggtatgat cgtcgtcgtg cttcatgtca catcgtccgg
     2461 ggtcttcgat gatgcaaccc tggagggacg gacgacacgc gttgatctgc gctcgatcca
     2521 cggcctcagg gagatcatcc gcctttattg ctatatttcg aagctcgtaa ccgggtcatt
     2581 ccggatacgc gcggtcaccc gtcgccatcg ggagtggtga cgggagtgcg cacacccggg
     2641 gttgaccccc cgacgcctcg gtatctcgag tgattcgtgg gcgctcggcg cgagacgcct
     2701 ggaaaggggg tgtacccgcc gtcagcgggc ttcgagggcg aagggagtgc caaacggacg
     2761 ttccacccag ggacgggtgg acgctccgcc cgtacagtct ctcacccccc tgcgcacctg
     2821 gttgcttcgg gcatgcgcca tgaagtctcc cgcaacgtga atgggtgcat atgcacacga
     2881 caggcgcacg gacggtctcc tctggctcgt tgccggattg acgagcgcgt gaatctcata
     2941 tactagggaa aatctggggg gtacccatgt cattgacgta ccgggatgca ggagtagaca
     3001 tagacaaaca gaaccagctc gtcagggcga tcaagtcgag cgtgaggtcc acccacggtc
     3061 ctgaggtcat aggcgggata ggcgggttcg cagggctctt caagctcgac accggggcat
     3121 acaccgaccc gatactcgta tcttccacgg acggcgtagg caccaagctc aagatcgcct
     3181 tcatggcgga caaccactcc actgtgggca tcgatctcgt ggccatggtg gtcaacgacc
     3241 tggttgtcgt gggtgcgcaa ccgttgttct tcctcgacta cttcgcgacc ggggcgctga
     3301 gcctggaacg cgccaaggcc gtcatatcgg gtatcacgag gggctgcgcc gaggccggat
     3361 gtgcgctcat cggcggagag acggccgaga tgcccggttt ctacagggaa gacgagtacg
     3421 acctcgccgg tttcggcgtg ggcgttgttg atgccgagaa gatcatcgat gggtccagga
     3481 tcgccgccaa ggacgtgatc atcgggatcc actcctcggg cctgcacagc aacgggttct
     3541 ccctggtgcg caaggtcctt ttcgagcggg ccaagctcag ggtggacgac tatctcgagg
     3601 atatcggcag gaccctcgca gaggaactcc tcacgcccac gcgcatctat gtcaagagcg
     3661 tgctcaacgt gatccgcagc ttcgagatca aggggatcgt tcacatcacg ggcggcggct
     3721 tcgtggacaa cataccgagg gttctcccgt cgaggtcgtg cgcccgcatc aggaaaggct
     3781 cgtgggaggt ccccccgatc ttcaggttca tccaggaggt cggcaagatc gaagatcacg
     3841 agatgtaccg cacgttcaac atgggtgtgg ggatgatcct gatcgtgtca ccgaaggacg
     3901 ccgacgatgt catgctcagg ctcaagggcc tcaaggagac ggcggacatc atcggctaca
     3961 tcgacgcgcg caccaaggga atggacccgg tgatcatctc atgatccgca taggcgttct
     4021 cgtctcgggg agcgggtcga acctccaggc catcatggat gcctgcgcct ccggtctcat
     4081 cgacggcacc gtcgccgtgg tgatcagcaa cgtgccggac gcctacgcac tcgtccgcgc
     4141 gcggaagatg tccgtcccga cggtcgtggt gcagcacgag gcgtatcccg acagggaatc
     4201 cttcgacgag gcgctcgcag gcaagctgga agagtaccag gtcgacctcg tgacccttgc
     4261 aggtttcatg cgtgtgctca ccccggcctt cctgaggcgc ttcccaggca ggatcatgaa
     4321 catacacccc gccctgttgc cctcattccc gggtcttcac gtgagacaga aggccttaga
     4381 ctacggggtc aggttctccg ggtgcacggt gcatttcgtg gacgagggcg tggacacggg
     4441 gcccatcatc atccaggccg ttgtccccgt gtaccctgac gacaccgagg aggagctgaa
     4501 cgagaggatc ctcaggctgg agcaccgcat ct
//
LOCUS       DAOSCR010000159         4523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12097_length_4523_cov_2.959266, whole genome shotgun sequence.
ACCESSION   DAOSCR010000159 DAOSCR010000000
VERSION     DAOSCR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12097_length_4523_cov_2.959266"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLT69_05235"
     CDS             complement(<1..922)
                     /locus_tag="PLT69_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ13877.1"
                     /translation="MTGFFEIAQILALAAVLGMAGLRLKQPLVIAFLAAGILAGPSFL
                     GIIHSYEQVELFAHIGIAVLLFIVGLKLDLHLIRTTGPVALATGLGQVVFTSNIGFGI
                     AVAMCMDTVSAAYVSVALTFSSTIIIVKLLSDKKEIDSLHGRIALGFLIVQDIVAILA
                     LIGLSSLGTSAHGGESRLAASLLVLAKGAGLVAGVWVVMRTVLPPLTRKIASSQEMLL
                     LFAVTWAMLVGAASDLLGLTKEVGAFLAGVALASTEYRDALSARLVTLRDFLLVFFFV
                     DLGARMEWSHFGPRVFEASVFSAFVLVGNPL"
     gene            1202..3265
                     /locus_tag="PLT69_05240"
     CDS             1202..3265
                     /locus_tag="PLT69_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ13878.1"
                     /translation="MDTGFVADLILILAMSIVVVLTFMRLRLPPILGFLLTGVIAGPH
                     ALGIVSGVEEVEALSELGVVLLLFSIGIEFSIKDLLASGRTVFMGGLVQVGLTTVLVS
                     IFCKALGLGTAEAVFSGVLVSMSSTAIVLKTLDDRYEITTPHGKNTLSILIFQDLLVV
                     ALMPLLPIAVHAKTGIEGGIGVLVAKAASLLAFLYVSYRWAVPWLLHQIARTKSRDLF
                     LLSVILLCIGIAWLTSRLGLSPALGAFLAGLIISESEYSQEALGRVLPLRDIFMSLFF
                     ISVGMLLDLGFVADHLPAITGLAVLVVGMKTFTGSLSTLALGFPFRSTVLTGLALAQI
                     GEFSFILSGTGLAMGLIGSGTYQTILAITLATMAATPFLINTSPALADLSVRMPLPRR
                     LLTGLKTSGALARGEARAVDMEGHLVVIGYGLNGRNVSKAAAMAGIPRVVIEMNPDTV
                     RSERKKGEPIVYGDATQEAVLRYVCTEKANVVVITIPDAASARRVTELVRKMNPHAHI
                     IARTRYVKEVEPLLALGADQVIPEEYETSIEIFSRALSEYMVPRDDIERLIADLRSSG
                     YSMLRSLSGEATTMTDLQRTLPDFKVHTLRVHPGCPIASRPLIETQVRKRHKVSILAI
                     RRGERTIVNPGGEDTLEPGDVIVVSGDLDSVLGLIPLLRGDTGKDGEAGTCPVDEKTR
                     HADGA"
     gene            3282..>4523
                     /locus_tag="PLT69_05245"
     CDS             3282..>4523
                     /locus_tag="PLT69_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13879.1"
                     /translation="MRKAIVILAALSVTAAALAAAVILAAQAYFSTSEGAARLLGFVN
                     TLYPGEIRGEKIEVDLVAQEVTILEPVILAPGGRKVLEADRAFLKTDLARLLRKDLVF
                     VTIEIDRPLFLLELDEDGWLTIESAFVEKTPGPAPVDVHIRRLVSRGATIEYRDDQAA
                     PVVRLDDTDLAMQASFERDSVLHLSSPRTSPSLFVPGRRVFLGSGKASCSIINDRITG
                     IDVHLTSGSTTAAMKGAVSEMSQKAKLDLEFTVNGAASYLENLFGTGWDLKGAVQATV
                     RASGAWDEPDLDISLAGPVATIKGRGIGPVSARGKIHERFLTIDHFCVPFASGDLKAQ
                     GSLDLRGVFPEGLFEGVKEEDALAYELSVTGTSLSLQEIPGVPEGVTGRLSPVVRIKG
                     SGVSPSKRRVEASFDAVCRALT"
BASE COUNT          787 a         1527 c         1490 g          719 t
ORIGIN      
        1 tgagcgggtt gccgacgagc acgaaggccg agaagaccga ggcctcgaag accctgggcc
       61 cgaagtgcga ccactccatg cgcgcgccga ggtccacgaa gaagaacacg aggaggaagt
      121 ccctgagcgt cacgagcctg gcgctcaggg cgtcgcggta ctcggtggac gcgagcgcga
      181 cccccgccag gaaggcgccc acctccttcg tcaggcccag gaggtcgctc gcggcgccca
      241 cgagcatggc ccaggtgacc gcgaagagga ggagcatctc ctgtgacgag gcgatcttcc
      301 tcgtgagcgg cggcaacacg gtgcgcatga cgacccatac cccggccacg agccccgcgc
      361 ccttggcgag cacgagcagg gaggcggcca ggcgggactc cccgccgtgc gcggaggtgc
      421 ccagcgagga gagccctatg agcgccagga tggcgactat gtcctgcacg atgaggaagc
      481 cgagggcgat cctcccgtgg agcgagtcta tctctttctt gtccgagagg agcttgacga
      541 tgatgatcgt gctcgagaac gtgagggcga cggagacgta cgccgcgctg acggtgtcca
      601 tgcacatggc gacggcgatg ccgaagccga tgttcgaggt gaagacgacc tgcccgaggc
      661 ccgtggccag ggcgaccggc cccgtggtgc ggatgaggtg gaggtccagc ttcagcccga
      721 caatgaagag caggacggct atccctatgt gcgcgaagag ctcgacctgc tcgtagctgt
      781 ggatgatgcc caagaacgac ggcccggcga ggatccccgc ggccaggaac gcgatgacga
      841 ggggctgctt cagcctgagg cccgccatgc ccaagaccgc ggcaagggcg aggatctggg
      901 ctatctcgaa aaaccccgtc atgcatcctc ccttccactg cgatgcgacc tgcccgattg
      961 taggcaaaag ccttgccgga ggaaagggtt ttcgagacag gcgaggccgc gcccgacgcc
     1021 gggccggccc tgcaccgggc atgcaagggg tcgcccgccg ggcgatcttg caggggcgcc
     1081 agggcgcacc cggccgtgac ggcggcacgc atgcgcagcg cccgagaatc gaaatgcctt
     1141 gccgccacgg ctttgagtct gtaaaaacat cccggttgcg ggtacaatgg aggcacttgg
     1201 gatggatacg ggtttcgtcg ccgatctcat actgatcctc gccatgagca tcgtggtggt
     1261 gctcaccttc atgcgcctga ggctcccgcc catcctcggg ttcctcctca ccggcgtgat
     1321 cgcgggcccc cacgcgctgg gcatcgtctc gggcgtcgag gaggtggagg ccctctcgga
     1381 actcggcgtg gtcctcctgc tcttctccat cgggatcgaa ttctccatca aggatctcct
     1441 ggccagcggc aggaccgtgt tcatgggcgg tctcgtccag gtggggctca ccacggtcct
     1501 ggtctccatc ttctgcaagg ccctgggcct cggcacggcc gaggcggtct tctcgggcgt
     1561 actcgtgtcc atgagctcga cggccatcgt gctcaagacg ctcgacgacc ggtacgagat
     1621 aaccaccccg cacgggaaga acaccctctc catcctcatc ttccaggacc tcctcgtggt
     1681 ggcgctcatg cccctcctgc cgatagccgt gcacgccaag accggcatcg agggcggcat
     1741 cggcgtgctc gtggcgaagg ccgcctccct gctcgccttc ctctacgtgt cgtaccggtg
     1801 ggcggtcccc tggctgctcc accagatagc acggaccaag agcagggacc tgttcctcct
     1861 gagcgtgatc ctcctctgca tcggcatcgc ctggctcact tcccggctgg gtctctcccc
     1921 cgcgctcggc gcgttcttgg cgggcctcat catctcagag tcggagtaca gccaggaggc
     1981 cctgggcagg gtgctccccc tcagggacat cttcatgagc ctgtttttca tctccgtggg
     2041 catgctcctc gacctgggtt tcgtggctga ccacctcccg gccatcaccg gtctcgccgt
     2101 gctcgtggtg ggcatgaaga ccttcaccgg gagcctgtcc acgctcgcgc tgggattccc
     2161 gttcaggagc acggtgctca ccgggctcgc cctggcccag atcggcgagt tctcgttcat
     2221 cctcagcggg acaggcctgg ccatgggcct catcggctcc gggacgtacc agaccatcct
     2281 ggccatcacg ctcgcgacca tggcagccac gccgtttctc ataaacacct cgccggcgct
     2341 ggccgacctg tccgtgcgca tgccgctgcc gcgtcgcctg ctcacggggc tgaagacctc
     2401 cggcgcgctc gcccggggag aggccagggc cgtcgacatg gagggtcacc tcgtggtcat
     2461 cggctacggg ctgaacggac ggaacgtctc gaaggccgcc gccatggcgg ggatcccgcg
     2521 cgtggtcatc gagatgaacc ctgacacggt gagatccgag cggaagaagg gcgagcccat
     2581 cgtctacggc gacgcgaccc aggaggcggt gctcagatac gtatgcacgg aaaaggccaa
     2641 cgtggtggtc atcaccatcc ctgacgccgc ctccgcccga agggtcaccg agctcgtgag
     2701 gaagatgaac ccccacgccc acatcatcgc gaggacgcgc tacgtgaagg aggtggagcc
     2761 gctgctggcc ctcggcgctg accaggtcat cccggaggag tacgagacat cgatcgagat
     2821 attctcgagg gccctgtccg aatacatggt cccccgggac gacatcgagc gcctcatcgc
     2881 cgatctccgc tccagcgggt acagcatgct caggagcctc tccggcgagg ccaccaccat
     2941 gactgaccta cagaggaccc tcccggactt caaggtgcac accctcaggg tgcacccggg
     3001 ctgccccatc gcctcgaggc cgctcatcga gacccaggtg cgcaagagac acaaggtgag
     3061 catcctggcg atccgcaggg gggagaggac catcgtcaac ccgggcgggg aggacacgct
     3121 ggagcccggc gacgtcatcg tcgtctcggg cgacctcgac agcgtgctcg gcctcatccc
     3181 cctgctcagg ggcgacaccg gcaaggacgg ggaagcaggg acctgtcccg tggacgagaa
     3241 gaccaggcac gcagacggcg cttgagaaac ccgggggacg catgcgcaag gccatcgtga
     3301 tcctcgcggc actctccgtc accgccgcgg ccctcgctgc ggcggtgatc ctcgccgccc
     3361 aggcgtactt ctccacgagc gagggggccg cacgcctgct cggcttcgtc aacacgctct
     3421 acccgggaga gatcaggggc gagaagatcg aggtcgacct cgtggcccag gaagtcacca
     3481 tcctcgaacc ggtgatcctg gcgcccgggg ggagaaaggt cctcgaggcg gatcgggcgt
     3541 tcctgaagac ggacctcgcc cgcctcctgc gcaaggacct cgtcttcgtg accatcgaga
     3601 tcgacaggcc cctcttcttg ctcgagctcg acgaggacgg ctggctcacc atcgagtcgg
     3661 ccttcgtgga gaagaccccg ggtccggccc ccgtcgacgt ccacatacgg cggctcgtct
     3721 cgagaggggc caccattgaa taccgggacg atcaggccgc acccgtcgtg aggctcgacg
     3781 acacggacct cgccatgcag gcatccttcg agcgggactc cgtgctgcac ctgtcttccc
     3841 ccaggacatc gccgtccctc ttcgtcccgg ggaggcgcgt cttcctggga agcggcaagg
     3901 cctcctgctc gatcataaac gacaggatca ccgggatcga cgtgcacctc acgtcagggt
     3961 ccaccacggc cgccatgaag ggggcggtct ccgagatgtc ccaaaaggcg aagctcgacc
     4021 tcgagttcac ggtgaacggc gccgcctcgt acctcgagaa cctcttcggg acaggctggg
     4081 acctgaaggg ggccgtgcag gccaccgtga gggcgagcgg cgcatgggac gagccggacc
     4141 tggacatcag cctcgcaggc ccggtggcca cgatcaaggg tcgcggcatc gggccggtct
     4201 cggcccgcgg gaagatccac gagcggttcc tgaccatcga ccacttctgt gtgcccttcg
     4261 cctcgggcga cctgaaggcg caaggctctc tggacttgag aggggtgttc cccgaggggc
     4321 tcttcgaggg tgtcaaggaa gaggatgcgc tcgcctacga gctctcggtc accggcacga
     4381 gcctgagcct tcaggagata cccggcgtgc cggaaggggt cacgggcagg ctgagcccgg
     4441 tggtgcgcat caagggttcc ggcgtgtcac cgtcgaagag aagggtcgag gcctcgttcg
     4501 atgcggtctg ccgtgccctg acc
//
LOCUS       DAOSCR010000160         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12215_length_4484_cov_3.819147, whole genome shotgun sequence.
ACCESSION   DAOSCR010000160 DAOSCR010000000
VERSION     DAOSCR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12215_length_4484_cov_3.819147"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(593..1096)
                     /locus_tag="PLT69_05250"
     CDS             complement(593..1096)
                     /locus_tag="PLT69_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOJ13880.1"
                     /translation="MSEQERVMQELVDAKGFQAVVAIVRIALGVMFVYAGLTKIVAPA
                     GFALAVYNYHILPAQLVNLTAVVLPWVEVTAGACLVLGLWTPGGALIVSSLLFVFTLA
                     LGFNLSRGLDIACGCFSSSPTAERITWWYLLRDGSLLVAALGVLFYDRGLFALGKRGT
                     GSSSKIG"
     gene            complement(1089..1568)
                     /locus_tag="PLT69_05255"
     CDS             complement(1089..1568)
                     /locus_tag="PLT69_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOJ13881.1"
                     /translation="MMDRIRQGVIQAFWMAALAAVIGVVFNLVRPAGIPFVGDWSPQA
                     VTRLHAGDLEVIHVDDAFALLSQGRALFLDAREEAAFAAGHIPGALNIPPGKAAGMLG
                     QVRAMLATGKTVIAYCYDVDCPLAADVVKELGKLGASPIKVMPEGWTGWLDRGYPYE"
     gene            complement(1565..1822)
                     /locus_tag="PLT69_05260"
     CDS             complement(1565..1822)
                     /locus_tag="PLT69_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ13882.1"
                     /translation="MAYRMHIEVTGPGCPFCKTLYKWVKEVVGEKGIDADVEHVTQLK
                     VVMRRFPFTPALEVDGEVVHRGKWLPNKEKIHALITRGKAS"
     gene            complement(2020..2259)
                     /locus_tag="PLT69_05265"
     CDS             complement(2020..2259)
                     /locus_tag="PLT69_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ13883.1"
                     /translation="MKKKLQILGTGCAKCIKLAANVEEAAKRAGIDYEIEKVTDINQI
                     MKFGVMMTPALAVDGKVKLVGKVLPPAELENLIKG"
     gene            complement(2292..3590)
                     /locus_tag="PLT69_05270"
     CDS             complement(2292..3590)
                     /locus_tag="PLT69_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ13884.1"
                     /translation="MNWKDEWKWLAGLAGVFLVLFYLPVGVPRFDNAVLEGLYLAKSY
                     AREHVILCLLPAFFIAGAIGVFLSQNTVMKYLGADAKKVLAYGVASVSGTILAVCSCT
                     ILPLFAGIYRMGAGLGPAIAFLYSGPAINVLAIILTARVLGIEMGVARAVGAIAFSVV
                     IGLVMHVIYRKEKDGQDGPKMMLGADDGMRPLWQNVLYFAAMIAVLVFANWGKPQSET
                     GIWHLVYASKWAITALSAAGLGVMLVAWFSVPVVKVAAVAAVTAAFSLTFPHTPTVGF
                     AVGVIGLSYLTSTQEGEMQEWFSGSWGFAKQIMPILFSGVLVAGFLLGRPGYEGIIPS
                     VWVSSLVGGNSLFANLVASVAGAFMYFATLTEVPILQGLMGSGMGKGPALALLLAGPA
                     VSLPNMLVIRSVIGTGKTIVYVTLVIVMATLSGYVYGTVF"
     gene            complement(3607..3960)
                     /locus_tag="PLT69_05275"
     CDS             complement(3607..3960)
                     /locus_tag="PLT69_05275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ13885.1"
                     /translation="MARRQDNAVMAADFKAFVFKALAHPLRVRLFEAVASGEKTVGEL
                     VAMTGEKEANVSRHLAVLRSARLVKTRKDGLNVYYANSLPCLVSMIECVNQALYMMAD
                     ENMKMACCLRSPIRS"
BASE COUNT          841 a         1516 c         1326 g          801 t
ORIGIN      
        1 ccttgtccat aagccaactt atagcacttt tcacgtgcca aagttgtcat gaacccaaca
       61 aaatatcata ccccaaaaag gcagatctgt caattcgtgc cattgactgt ctcaaataga
      121 ctatcctgcg gcatcttcgt ggccgcaccc agcgttctac cgcaggcatc taagccctcg
      181 acgtcgacga ggcgacgggt ggccgccgtg agctgtaccc ccgcggcatc atcgaggggg
      241 tgatcaggaa acgaccctct tgtggacacc ccatcccgaa gttccagatc gggataggat
      301 gcctcacggc ctcagtcact ggatttctgg cgggcgtcgc ctctcgggct gcagtggctc
      361 cccctattca tcattccgcc tggggaatct cactcctacg tgcgatgcgg caggggctgc
      421 ggggccgcca tgtcccctgg tgtttgcgcg ggtgttctcc cagtttcgcg ccttgtccat
      481 ggaaaccccc tctccctgca ggagacagct catggagcgg gagccgaccg ggcatgaact
      541 tctctggcag tatgtttctc gagcagcccc tggaacgcgt cggaccgctc gttcacccaa
      601 tctttgagga tgagcctgtg cccctcttcc ccagggcgaa gagccccctg tcgtagaaga
      661 gcacgccgag cgcggccacg agcagggagc cgtcccgcag caggtaccac caagtgattc
      721 tctccgccgt gggggagctg ctgaagcacc cgcaggcgat gtcgaggcca cgggagaggt
      781 tgaagccgag cgcgagggtg aagacgaaga gcaggcttga gacgatgagc gcgccgcccg
      841 gcgtccacag gccgagcacc aggcacgcac cggctgtgac ctccacccag ggcaggacga
      901 cggccgtgag gttcacgagc tgggcgggca ggatgtggta gttgtagacc gccagggcga
      961 agcccgcggg cgcgacgatc ttggtgaggc ccgcatagac gaacatgacg ccgagcgcga
     1021 tgcgcacgat cgccaccaca gcctggaaac ccttcgcatc cacgagttct tgcatgacgc
     1081 gttcctgctc actcataggg ataccccctg tccagccagc ctgtccagcc ctcgggcatg
     1141 accttgatcg gcgaggcacc cagcttgccg agttccttga ccacgtccgc ggccaggggg
     1201 cagtcaacgt cgtagcagta tgcgatcacg gtcttgccgg tcgcgagcat ggcacggacc
     1261 tgcccgagca tgccggcggc cttccctggg gggatgttca gcgcgccagg gatgtgcccc
     1321 gctgcgaagg ccgcttcctc ccgtgcgtcc aggaacaggg ccctgccctg cgacagcagc
     1381 gcaaaggcat cgtccacgtg gatgacttcc aagtcccccg cgtgcagcct cgtcaccgcc
     1441 tgggggctcc agtcacccac gaaggggatg cccgcaggcc tgaccaggtt gaacaccacc
     1501 ccgatgacgg cggcgagcgc cgccatccag aaggcctgga tcacaccctg cctgatcctg
     1561 tccatcatga agctttcccc cttgtgatga gggcatggat cttttccttg ttcgggagcc
     1621 acttgccccg gtgcaccacc tcgccgtcca cctcgagcgc cggggtgaag ggaaagcgcc
     1681 tcatcacgac cttcagctgg gtgacgtgtt ccacgtccgc atcgatcccc ttctcgccca
     1741 ccacctcttt cacccattta tagagcgtct tgcagaaggg gcaccccggg ccggtcacct
     1801 cgatgtgcat tctgtaagcc atgtaactcc ccccatttca accccggaac cggttgggca
     1861 ttgttcagcc ccagactcac ggatccctga caaggtgagc ggtcatgaga cacaccccga
     1921 gagggatgat ctccagtctt ctcgtgggcc actttcccgt gggcattcca acgccactca
     1981 tgggctcacc tcgtcgatga accctgcccg gggcattcct caacccttga tcaggttttc
     2041 gagttcggca ggcggcagga ccttgcccac gagcttcacc ttgccgtcca cggcgagcgc
     2101 cggggtcatc atgacgccga acttcatgat ctggtttatg tccgtgacct tttctatctc
     2161 gtagtcgata cccgcccgct tcgccgcctc ctcgacgttg gcggcgagct tgatgcactt
     2221 ggcgcacccg gtacccagga tctggagttt cttcttcatg acggcttcct cccctttctc
     2281 tcttggtccg cttaaaagac ggttccgtac acatacccgg acagtgtcgc catgacgatg
     2341 accagcgtta catacacgat tgttttcccc gtgccgatca cggaccggat cacgagcatg
     2401 ttcggcagcg acacggccgg gccggccagc agcagggcca gcgcaggccc cttgcccatg
     2461 cccgagccca tgagcccctg gaggatgggc acctcggtga gcgttgcgaa gtacatgaag
     2521 gcgccggcca ccgaggcgac gaggttggcg aacagggaat tgccgcccac gagcgagctc
     2581 acccataccg aggggatgat cccctcgtag ccgggccttc ccagcaggaa gcccgccacc
     2641 aggacgccgg agaacaggat gggcatgatc tgcttggcga agccccagga accggagaac
     2701 cactcctgca tctcaccctc ctgggtgctc gtgaggtacg acaggccgat gacgcccacc
     2761 gcgaacccca ccgtgggggt gtgcgggaag gtcagggaga aggccgcggt gacggcggcc
     2821 acggccgcca ccttgaccac cggcacggaa aaccacgcca cgagcatgac gcccaggcca
     2881 gccgcgctga gagccgtgat ggcccatttc gatgcgtaga ccaggtgcca gatgcctgtc
     2941 tcagactggg gcttgcccca gttggcgaac acgagcaccg cgatcatggc cgcgaagtac
     3001 agcacgttct gccacagggg ccgcatgccg tcgtccgcgc ccagcatcat cttcggcccg
     3061 tcctggccgt ccttctcctt gcggtagatc acgtgcatga cgagcccgat gacgacgctg
     3121 aaggctatgg cgcccacggc ccgtgcgaca cccatttcga tgccgagcac ccgcgcggtc
     3181 aggatgatgg ccagcacgtt gatggcgggc ccggagtaga ggaaggcgat cgccgggccg
     3241 agcccggcgc ccatgcggta gatgcccgcg aagagcggca ggatggtgca ggagcacacg
     3301 gcgaggatgg tgccggacac cgacgccacg ccgtaggcca gcaccttctt tgcgtcagct
     3361 cccaggtact tcatgaccgt gttctgggac aggaacactc cgatggcgcc cgcgatgaag
     3421 aaggcgggca gcaggcacag gatcacgtgt tcccgcgcat aggacttcgc caggtacagg
     3481 ccttcgagga cggcgttgtc gaaccggggc acgcccacgg ggaggtagaa cagcaccagg
     3541 aaaacacctg caagcccggc aagccacttc cattcatctt tccagttcac gggtatttct
     3601 ttcctttcac gagcgtatgg gagacctgag gcagcacgcc atcttcatgt tctcgtccgc
     3661 catcatgtag agcgcctggt tgacacactc gatcatcgag acgaggcatg gcagggagtt
     3721 ggcatagtac acgttgaggc cgtccttcct cgtcttcacg aggcgggccg acctgagcac
     3781 cgccaggtgc ctggagacgt tcgcctcctt ctcgcccgtc atggcgacga gctcgccgac
     3841 cgtcttctcc cccgaggcga cggcctcgaa gagacgcaca cgcaaaggat gcgcgagcgc
     3901 cttgaagacg aaggccttga agtccgccgc catcaccgca ttgtcttgcc gcctcgccat
     3961 ggaacaggcc cccttatgtt tactagttta ctatttagtt gattagtagt tctcggtcaa
     4021 ggccctacca caccgcagtc cggctgtcaa gcaaacccac aaccgcgcct tgcgccgggc
     4081 cttgcctgtc ctcgggtgca gcatggctga aaacatctcg atcatccatc gcccggggca
     4141 atcggcaacc cctgcgctcg agcatgcatt gcctgggtct cgccaccaag acaaccaggc
     4201 cccccgtcgt acacgtctgc cgggtttccc cccggcaggt tcacgccctc gcgcctgatg
     4261 cccctgccag gaccacggcc aacccgtcca caaggcgtgt cacgggatcg gcaaccatcg
     4321 ggctcgagca tgcccccggc tgccggcatc ctcgtcccac gactttcaca cttactgcgg
     4381 caccatccgg agcagagcct gtgccttcgc gttgttggtc aacgtggggc acgggcttct
     4441 ccaggccgcg cgaacacggg atgaacgcgc gggagaaggc gaag
//
LOCUS       DAOSCR010000161         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12366_length_4443_cov_3.375114, whole genome shotgun sequence.
ACCESSION   DAOSCR010000161 DAOSCR010000000
VERSION     DAOSCR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12366_length_4443_cov_3.375114"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLT69_05280"
     CDS             <1..702
                     /locus_tag="PLT69_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13886.1"
                     /translation="MFSIPHTAETDTKYSLGGALLITRPENKGRTAVEMVTTDTRRLS
                     LSRYVMDGKISMGEGIIVPRKGLQEIKKLFDEKEQGAGVVITPTSLYYVSETTSATVR
                     LFEGTFPEYRNVVDLSTYPNTVRMNAQELLGVLRVCAVMVSDLMNCVRFSFKKDKTIV
                     YANNPEQGEVDTVISSELDGDEVDISFNPRYFIDCLAFIEQDALLHLRGSQGPCLVMD
                     ADHDDCKWIIMPMRY"
     gene            747..3116
                     /gene="gyrB"
                     /locus_tag="PLT69_05285"
     CDS             747..3116
                     /gene="gyrB"
                     /locus_tag="PLT69_05285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOJ13887.1"
                     /translation="MQEYTADDIKVLDGFEAVRLRPAMYIGSTSSQGLHHLVFEVVDN
                     SVDEAIAGYCDYIKVIIHMDESITVMDNGRGVPVDIHKETGRPAAEVVLTTLHAGGKF
                     DNSIYKVSGGLHGVGVSVVNALSESLEVHIFRDGYRYNQKYERGVPVTDLVRLEATEK
                     RGTTVHFKPDPDIFETTSFEYDILANRLRELSFLNKGLKIELEDERTDTVDTFFYKEG
                     LKDFIAYLNAKKKPIHQPIHFKRNKGDVVLECVLQYNTSYTESLFSFANNINTHDGGT
                     HVIGFRKALTRCINAYARDRGFLKNTKITIQGDDTREGLCAVISVLLPRPQFEGQTKG
                     KLGNSDIVGIAESITNEAISSYLNENPSEAAAIVKKILDSAQAREAARKARELTRRKS
                     AMENTLLPGKLADCQERDPALCELFIVEGDSAGGSAKQGRDRKNQAILPLKGKILNVE
                     KARIDKMLSNQEIKALVTALGTGIGSDSFDISKLRYHKIVIMTDADVDGAHIRTLLLT
                     FFFRQLPEIIERGHLYIAQPPLFKIMKGKKMAYVLNEEALDEFLLKNITRDFVLKGAI
                     EITGAELARYLKLINRRKNILVNYEMRQMDERIVQSVSYLDAQTLREGFDSARIKEET
                     LTIIQEWFPYIGPVGCDAMVDHLVFTSVKNGIRKETVIDPKLLESKGFEELQKIHQQL
                     KAIGKPPFKVFKGDVSFDVVNLTDAAARIYDEAKKGYTIQRYKGLGEMNPEQLWETTM
                     DPERRTLLQVTIDDALETDQIFSTLMGDDVEPRREFIEKNALKVVNLDI"
     gene            3148..>4443
                     /locus_tag="PLT69_05290"
     CDS             3148..>4443
                     /locus_tag="PLT69_05290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HOJ13888.1"
                     /translation="MNSTKITTVAIDQEMRRSYLDYSMSVIIGRAIPDARDGLKPVHR
                     RILYAMMEAGNTHNKPYKKSARIVGDVIGKYHPHGDAAVYDAIVRMAQPFSMRYTLVD
                     GQGNFGSIDGDPPAAMRYTEIRMSRLAEEMLADIDKDTVGFHPNYDESLQEPDVLPSR
                     IPALLLNGSSGIAVGMATSIPPHNLAELSRALVTLIENPACELRDIMKVLPGPDFPTG
                     GIIFDGGEIAKAYATGRGIIILRGKVTREEEDGRTRLIITEIPYTVNKSDLVERIAIL
                     ANEKKIDGISTIRDESDKDGMRVVVELKKAVPAEVIENQLYKLTSLQSSFSMNMLAIH
                     NGRPCVMGLMDMLRAFLDFREEVVTRRSLFELNKAKDRVHILEGLLKALEHIDEIVAI
                     IKASADVADARLRLMERFSFTEAQAQAILDMRLQRLTGLE"
BASE COUNT         1097 a         1277 c         1289 g          780 t
ORIGIN      
        1 atgttctcga taccccatac ggctgagacc gataccaagt actctctcgg cggcgccctc
       61 ctgatcacca ggccggagaa caaaggacgt accgccgtcg agatggtcac gacggacacc
      121 aggaggctct ctctctcccg ctacgtgatg gacgggaaga tctccatggg cgaagggatc
      181 atcgtgccga ggaaaggtct ccaggagatc aagaagctct tcgacgagaa agagcaaggg
      241 gccggcgtgg tgatcacccc gacctccctc tactatgttt ctgaaacgac gagcgccacg
      301 gtgaggctgt tcgagggcac cttccccgag tacagaaacg ttgtggacct gtccacctac
      361 ccgaacacgg tacgcatgaa cgcccaggaa ctcctcggcg tcctcagggt gtgcgcggtg
      421 atggtctcgg atctcatgaa ctgcgtgagg ttctcgttca agaaggacaa gaccatcgtc
      481 tacgcgaaca accccgagca gggggaggtg gacaccgtca tctcttcgga actggatgga
      541 gacgaggtgg acatttcctt caatccgaga tattttatcg actgcctcgc gttcatcgag
      601 caggacgctt tgctgcacct gaggggaagc cagggacctt gcctcgtcat ggatgccgat
      661 catgacgact gcaagtggat catcatgccc atgagatact gatcccgagc cgccaccagg
      721 ctcgtagggg tcatcaagga aaggatatgc aggaatatac agcagacgat atcaaggttc
      781 ttgacgggtt cgaggcggtc aggctgcgac ccgcgatgta catagggtcg acctccagcc
      841 aggggttgca tcacctcgtc ttcgaggtgg ttgataacag cgtcgacgag gccatagccg
      901 gatactgtga ttacatcaag gtcatcatcc acatggacga gagtatcacg gtcatggaca
      961 acgggcgcgg tgtgccggtc gacatccaca aggagacggg gaggcccgcc gccgaggtcg
     1021 tactgaccac gctgcatgca ggcggcaagt tcgataactc tatctacaag gtatccggag
     1081 gactccacgg cgtcggcgtt tctgtggtga acgccctgtc tgaaagcctg gaggtgcata
     1141 tattcaggga cggttaccgc tacaaccaga agtacgaacg cggcgtgccc gtcaccgacc
     1201 tcgtccgcct ggaggcgacg gagaagagag ggacgactgt tcacttcaag cccgaccctg
     1261 acatcttcga gaccacaagc ttcgagtacg acatccttgc caaccgcctc agggagcttt
     1321 ccttcctcaa caagggtctc aagatagagc tcgaggacga gaggacggac accgtcgaca
     1381 cctttttcta caaggagggg ctcaaagact tcattgcgta cctgaacgcc aagaagaagc
     1441 ccatccacca acccatccac ttcaagagga acaaggggga cgtcgttctc gagtgtgttc
     1501 tccaatacaa cacgtcctac acggaaagcc tcttttcctt tgcaaacaac atcaacacgc
     1561 acgacggcgg cacccatgtg atcggcttca ggaaggccct cacgaggtgc atcaatgcgt
     1621 atgcgaggga caggggtttt ctcaagaaca ccaagatcac catccagggc gacgacacga
     1681 gggaaggatt gtgcgccgtc atcagcgtcc tgttgcccag gccgcagttt gagggtcaga
     1741 caaagggaaa actcgggaac tccgacatcg tggggatagc agagagcatc acgaacgagg
     1801 ccataagctc gtacctcaac gagaatccct ccgaggccgc ggccatcgtg aagaagatcc
     1861 tcgacagcgc ccaggcgagg gaagcggcca ggaaggcgag ggaactcacg aggagaaaga
     1921 gcgccatgga gaacacgctc ctgcccggaa agctcgcaga ctgccaggag agagacccgg
     1981 ccctctgcga gctgttcatt gtcgagggtg attcggcggg aggcagcgcc aagcaaggca
     2041 gggacagaaa gaaccaggcc atcttgccgt tgaagggtaa gatcctcaac gttgaaaaag
     2101 ctcggataga caagatgctc tccaaccagg agatcaaggc cctggtgact gcgctcggca
     2161 ccggtattgg atccgacagt ttcgacatca gcaagctaag gtaccacaag atcgtcatca
     2221 tgaccgatgc cgacgtagac ggcgcgcaca tcaggaccct cctcctcacc ttcttcttca
     2281 ggcaactgcc ggagatcatc gagcgtgggc acctctacat cgctcagccg ccccttttca
     2341 agatcatgaa ggggaagaag atggcctacg tcctcaacga ggaggccctg gacgagttcc
     2401 tcctgaagaa catcacgagg gacttcgttc tcaaaggcgc catagagatc accggtgcag
     2461 agctggccag gtatctcaag ctcatcaacc gaaggaagaa tatactcgtc aactacgaaa
     2521 tgagacaaat ggatgaacgc atcgtgcaat ccgtttccta tctcgatgcg caaaccttgc
     2581 gggaagggtt cgactctgca aggatcaagg aggaaacgtt aacgatcata caagagtggt
     2641 tcccatacat agggcccgtg ggatgcgatg ccatggtcga tcacctcgtg ttcacgtccg
     2701 tgaaaaacgg gatacgaaag gaaacggtga ttgacccgaa gctcctggaa tcgaaggggt
     2761 tcgaggagct ccagaagatc caccagcagc tgaaggccat cggcaaaccg ccgttcaagg
     2821 tgttcaaggg cgatgtctcc tttgacgtcg tgaacctcac cgatgccgcg gcaaggatct
     2881 atgatgaggc caagaagggc tacacgatcc agaggtacaa ggggctcggc gagatgaatc
     2941 ccgagcagct gtgggagacg accatggatc ccgagcgaag gacccttctc caggtcacca
     3001 tcgatgacgc actcgagacc gaccagatct tctccacgct catgggagac gacgtggaac
     3061 cgcgtcgaga gttcatcgaa aagaacgcat tgaaggtcgt gaacctcgat atctgacctg
     3121 tccccttccc gcaataaagg agccttgatg aattccacga agatcacgac ggtagccata
     3181 gaccaggaaa tgagacgctc ttacctcgat tactccatga gcgtcatcat cgggagggcc
     3241 atcccagacg cgagagacgg gctcaagccc gttcacaggc ggatcctgta cgccatgatg
     3301 gaggccggca acacgcacaa caagccatac aagaaatcgg cccgtatcgt aggcgacgtg
     3361 ataggcaagt accatcccca cggggatgcg gcggtctacg atgccatcgt gcgcatggcc
     3421 cagccgtttt ccatgagata cacgctcgtg gacggccaag ggaacttcgg gagcatagac
     3481 ggcgaccctc ccgctgccat gcgttatacg gaaatacgga tgtccaggct cgcagaggag
     3541 atgcttgcgg atatcgacaa ggacaccgtc ggattccatc ccaactacga cgagtctctc
     3601 caggagcccg acgtgctccc ctcgaggata ccggccctgc tcctgaacgg gtcctcgggc
     3661 atagcggtgg gcatggcgac gagcataccg ccgcacaacc tagcggaact ctcgagggcc
     3721 ctggtcaccc tcatagagaa tcccgcctgc gagctccgcg atatcatgaa ggtgctcccg
     3781 ggtcccgact tccccacggg tgggatcatc ttcgacggcg gcgagatcgc aaaggcttac
     3841 gccaccggca gggggatcat catcttgagg ggcaaggtga cgagggagga ggaagacggc
     3901 aggacgcgcc tgatcatcac ggagatcccg tacaccgtga acaagtcgga cctcgtcgag
     3961 aggatcgcga tccttgcgaa cgaaaagaag atcgacggca tctcaaccat ccgggacgag
     4021 tcggacaagg atggcatgcg ggttgtcgtg gagctcaaga aggccgttcc ggccgaggtg
     4081 atcgaaaacc agctctacaa gctcacatcc ctccaaagct ccttctccat gaacatgctc
     4141 gccatccaca acggcaggcc gtgcgtcatg gggctgatgg acatgctccg ggccttcctc
     4201 gacttcaggg aggaggtcgt cacgagacgc agcctgttcg agctgaacaa ggccaaagac
     4261 cgcgttcata tcctcgaagg tctcctgaag gccctcgagc acatcgacga gatcgtcgcc
     4321 atcatcaagg cctcggctga cgtggcggat gcgcgcctga ggctgatgga gaggttctcg
     4381 ttcacggaag cccaggccca ggccatcctt gacatgcgcc tccagaggct gaccgggctc
     4441 gag
//
LOCUS       DAOSCR010000162         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12379_length_4440_cov_3.314481, whole genome shotgun sequence.
ACCESSION   DAOSCR010000162 DAOSCR010000000
VERSION     DAOSCR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12379_length_4440_cov_3.314481"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLT69_05295"
     CDS             complement(<1..786)
                     /locus_tag="PLT69_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOJ13889.1"
                     /translation="MEPLLSGVKILDFTYLLPGPFATMILSDMGAEVLRVESHTRMDM
                     ARLAPPFVDEACTVSCMHAYLNRNKRSIALDLKRAEAVEIVRRLIKEQGYDIVVEQFR
                     PGAMARLGLSYDDLRELDGGLIYCSITGYGQTGPYRDRAGHDINYLSLAGVMSYSGTP
                     EGGPCLMGVQVADVGSGSKDAAIAILAAVVSRTKTGKGRYIDVSMTDGVYPFHAVSGI
                     GCLVGAPEPGYATEILNGCSLYGFYRTACGRHISFGGLEPQFLA"
     gene            1331..2461
                     /locus_tag="PLT69_05300"
     CDS             1331..2461
                     /locus_tag="PLT69_05300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ13890.1"
                     /translation="MKVLVVTTSYPDRPGAQRGIFIRELCRALTGKGAEVMVVTPRVD
                     PSSPLRENDQGIEVRRFTYPTGGRRLHQTESIPVFPMAVFMVSGLASTLRAVRSFRPD
                     VIHGNWIVPTGLIASIAGLSSLTPVVNTARGMDTRLRKNPAVWPLFDLAVRLSGRVTV
                     VSEAMRAIPGLEAAEVIPSGIKDQFFEVSPDPSSQTVLYTRSLEPVYGADTIIRSIPH
                     VLEKAPGARFVIAGTGSLAGHLEAMASELGVRDRVTFLGHVPHESVPALLSESRVFVS
                     TATEDGTSVALMEAMAAGLIPVATDIAPNRTLIEHETDGFLFRPKDAPGLALMITRAL
                     SGEIREEAIASKRADLKRRIPWSVVADRFVLGYNQLLERSRR"
     gene            2903..>4440
                     /locus_tag="PLT69_05305"
     CDS             2903..>4440
                     /locus_tag="PLT69_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ13891.1"
                     /translation="MLGILRELFTYLPPRRTFRFTVLLAGMVLVAVLETLTAGVISFY
                     AASFANPGLIIQDYIPKAQKVFPWIPSLDTKGLILALSLTVIALVVVKNAAAAVVTYA
                     TNLYTANVSGHLGRLMFSGFLDMPYEWHTRHFSADLIQNVGWSQYFGLLLRSVLKFVS
                     DTLIVAILLTTILVANPAISVLVFLTIGGASLAIISHARRAIDRLTERQREFNISINR
                     QVSMAFHGIKELKVYNRTEGFIDAYAEEFLGLARIEARLGFVNQLPGWLLEILGVGLL
                     SSAIMIMFLWFGDSTAKITGTIALLAVTSWRVIPAMGRILNSLNSMRESLPYVQTGLG
                     RLREVHARPRPARTGRPARKRFEEAFRVENVTFGYQGANTFAVEDLTLTIPRGQVLGI
                     IGTSGAGKSTLADITAGLLPPATGELLLDGRRLAGEDLAGWQSLIGYVPQTPYILPGT
                     LAENVAFGVRKEEIDMEKVMRCCRMAAMDDFLHELPQGIETYIGERGVKLSGGQRQRV
                     AIARA"
BASE COUNT          788 a         1462 c         1423 g          767 t
ORIGIN      
        1 cgccaggaac tgcggctcga gcccgccgaa ggagatgtga cggccgcagg cggtgcggta
       61 gaacccgtag agcgagcagc cgttgaggat ctcggtggcg tatcccggct cgggtgcgcc
      121 cacgaggcag ccgatcccgc tcaccgcgtg gaagggatac acgccgtctg tcatggagac
      181 gtctatgtac cgcccctttc ctgttttcgt cctcgatacg accgcggcaa ggatcgcgat
      241 ggccgcgtcc ttggaacccg accccacgtc ggccacctgg acgcccatga ggcagggccc
      301 gccttccggg gtcccggagt agctcatgac gcccgcgagc gagaggtagt tgatgtcgtg
      361 ccctgcccgg tccctgtatg ggcctgtctg gccgtagccg gtgatggagc agtagatgag
      421 gccgccgtcc agctccctga gatcgtcgta ggagagcccg agcctcgcca tcgcgccggg
      481 cctgaactgc tccaccacga tgtcgtaacc ctgttccttg atcagcctcc gtacgatctc
      541 gacggcctcg gcgcgcttga ggtcgagcgc gatggaccgc ttgttgcggt tgaggtaggc
      601 gtgcatgcag gagaccgtgc acgcctcgtc aacgaacggc ggcgcgagcc tcgccatgtc
      661 catgcgggtg tgcgactcca ccctgaggac ctccgccccc atgtccgaca ggatcatggt
      721 ggcgaaggga cccggcagga ggtaggtgaa gtcgaggatc ttcactccgg aaagcagggg
      781 ttccatgcct ggtgcttcct ctcctcgtta cgggcgttgt gccctttcag aaccatgccg
      841 catgcatgcc gcacacgaaa acgcgttctt ccactcgatc ggctcgaggc ataacccggc
      901 aaagcgccgt ctatcttcac ccggcttcac gccggcattt cactcacgcg atcgcaaccg
      961 tcgaatcact ctacgctatt atccaaccgt tgacaaccgg ttgtcaacag ttttcgtatg
     1021 tttgtaatac atcattacca ccgggccgcg aggccgccgg gtcaccgcgc acccgcccga
     1081 ccctgccgat catgatgcgg tgcggatcca ggacctgggc cgcaccgagc gagagggatc
     1141 gtcatccagg aaagggaact ctcacggaag gcacgacgaa tgccccggag aaggccgtcg
     1201 ccctccaagc gtgcattcct cttgtcgtgc ccggcgtgca ggggatgaat ggcgaaacgg
     1261 cgagcgatgg aatgagcgaa agcgtgcatt cctcttgtca tgcccgacgt gctgggatag
     1321 gacttcgttc atgaaggtgc tcgttgtgac cacctcgtac cccgacaggc ccggcgcgca
     1381 gcgggggatc ttcatcaggg agttgtgccg ggcactcacg ggcaaaggcg ccgaggtcat
     1441 ggtcgtgacg cccagggtgg acccgtccag cccgcttcga gagaacgacc agggcatcga
     1501 ggtgaggagg ttcacgtacc cgacgggcgg aaggcggttg caccagacgg agtccatacc
     1561 tgttttcccc atggcggtgt tcatggtctc cggcctggcc tcgaccttgc gggccgtgcg
     1621 ttctttcagg cccgacgtca tccacgggaa ctggatcgtg cccaccggtc tcatcgcttc
     1681 catcgcgggg ctttcgagcc tcacccccgt ggtcaacacg gcgcgcggca tggacacccg
     1741 cctcaggaag aaccctgcgg tctggcccct gttcgacctg gcggtgaggc tctcgggcag
     1801 ggtgacggtc gtctcggagg ccatgcgggc gatccccggg ctcgaggcag ccgaggtcat
     1861 accatcgggg atcaaggacc agttcttcga ggtgtcgccc gacccctcgt cacaaaccgt
     1921 gctgtatacc cggagcctcg agcctgtcta cggggccgac acgatcatac ggtcgatacc
     1981 ccacgtcctc gagaaggccc cgggggcgag gttcgtcatc gcgggaacgg gcagcctggc
     2041 gggacacctc gaggccatgg cctctgaact cggggtgaga gaccgggtga cgttcctcgg
     2101 gcacgtcccc cacgagagcg tgcccgcgct cctttccgaa tcgagggtct tcgtctcgac
     2161 cgcgacggag gacgggacct cggtcgccct catggaggcc atggccgcgg ggctcatacc
     2221 cgtggccacg gatatcgctc ccaacaggac cttgatcgag cacgaaaccg acgggttcct
     2281 cttcaggccc aaggatgcac ccgggctcgc cctcatgatc acgagggcgc tctcgggcga
     2341 gatcagggaa gaggccatag cctcgaagag ggccgacctc aagagacgca tcccctggag
     2401 cgtcgttgcg gaccggttcg ttctcggcta taaccagctt cttgaacgat cgaggcggta
     2461 gggcgggcct gtagtttcgc ccgcatcagg acatgagacc cgcgcacaag gagaaagaga
     2521 gcggactcga ggcggctgcc gggcgtcaac gactgcaccc ggaaaggcgg agccatgacc
     2581 atgaagggat ttccgggtcc gcccgtccat cccggccgag gacctctcgc acctgccccg
     2641 ggcgggcccg caccggaggc cgcagggccc atgcacggcg ggcagtcgtg tcaagatcga
     2701 caggaaaggc acgcaacagt cccgcggcga ccacgggcga agtggccctg gttatcgtcg
     2761 ccagcccctg tcgcatcgag cgtcacgggc tgaaagggct tttgggcatg ggagggtcac
     2821 ggtccccctc ccagtcggtc gggaaggccc gttcttggaa ggtccaaccg ttcgggacac
     2881 ccaccggcag gacgctaccg gcatgctcgg catactgaga gagctgttca cctatctgcc
     2941 gccccggagg acgttcaggt tcacggtcct tcttgccggg atggtcctgg tggcggtcct
     3001 ggagaccctc accgccgggg tgatctcctt ctatgcggcc tcgttcgcca accccggcct
     3061 catcatacag gactacatcc ccaaggctca gaaggtcttt ccctggatcc cctccctcga
     3121 cacgaagggg ctgatccttg ccttgagcct caccgtgatc gcgctcgtgg tggtgaagaa
     3181 cgcggccgcc gcagtcgtga cgtatgccac caacctctac acggcgaacg tgagcggcca
     3241 cctcggcagg ctcatgttca gcggtttcct cgacatgccg tacgagtggc acacccggca
     3301 cttctcggcc gacctcatac agaacgtcgg ctggagccag tacttcggcc tcctcctgag
     3361 gtctgtgctc aagttcgtga gcgacaccct gatcgtggcc atcctgctca ccaccatcct
     3421 ggtcgcaaac ccggccatct cggtcctcgt attcctgacc atcggcggag cctccctggc
     3481 catcatcagc cacgcgagaa gggccatcga ccggctgacg gaaaggcagc gcgagttcaa
     3541 catctccatc aaccgccagg tctccatggc cttccacggc atcaaggaac tgaaggtcta
     3601 caacaggacc gagggcttca tcgacgcgta cgcggaagag ttcctggggc tggcgaggat
     3661 cgaggcccgg ttgggcttcg tgaaccagct gcccggttgg ctgctcgaga tcctgggcgt
     3721 gggcctcttg tccagcgcca tcatgatcat gttcttgtgg ttcggcgatt ccacggcgaa
     3781 gatcacgggc accatcgcgc tgctcgccgt cacctcgtgg agggtcatcc cggccatggg
     3841 ccgcatcctg aacagcctga actcgatgag agagtccctt ccctacgtgc agaccgggct
     3901 cggccgcctc cgtgaggtgc acgcgcgacc gaggcccgcc cgcacgggca ggcccgcccg
     3961 caagcgtttc gaagaggcgt tcagggtgga gaacgtcacg ttcgggtacc agggcgccaa
     4021 caccttcgcc gtcgaggacc tcacgctcac catcccgagg gggcaggtcc tcggcatcat
     4081 agggacctcc ggcgcgggga agagcacgct cgcggacatc acggcggggc tcctgccccc
     4141 cgcgacgggc gaactcctgc tcgacggcag gaggcttgcc ggtgaggacc tggcgggctg
     4201 gcagtccctg atcggctacg tgccccagac cccgtacatc ctgccaggca cgctggcaga
     4261 gaacgtggcg ttcggcgtca ggaaggaaga gatcgacatg gaaaaggtga tgaggtgctg
     4321 caggatggcc gccatggacg acttcttgca cgagctcccc caagggatcg agacctacat
     4381 cggtgagcgg ggggtcaagc tctcgggggg ccagcggcag agggtcgcca ttgcgcgcgc
//
LOCUS       DAOSCR010000163         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12396_length_4434_cov_2.906828, whole genome shotgun sequence.
ACCESSION   DAOSCR010000163 DAOSCR010000000
VERSION     DAOSCR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12396_length_4434_cov_2.906828"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLT69_05310"
     CDS             <1..693
                     /locus_tag="PLT69_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13892.1"
                     /translation="PIREGYGLNETTGITHISDEENFKLGTVGRSLPESEVKIAEDGE
                     ILIRHGGVFKGYFKEPAATAAALEGGWFHTGDVGEIDDEGYLKITDRKKDLIITAGGK
                     NVAPQYIENLLKFSPYINDAVVIGDRRKYLTALIVIDEENVMKYAQDNKVQYTTFASL
                     TRAEEIVKLIEAEVAKVNRQLARVENIRKFRLLDKKLYTEDGEVTPTMKVKRAAIARQ
                     YKDLIEEMYAED"
     gene            1292..2452
                     /locus_tag="PLT69_05315"
     CDS             1292..2452
                     /locus_tag="PLT69_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13893.1"
                     /translation="MIIRRYAVFVLAAAVLALFAAACSNDDSGGPAAPEELTTCISVS
                     AGENHTLAVDSDGRLWAWGHNRYGQLGDGTWEDSNVPVPVGHDTDWKSVSAGNHFSVA
                     VKHNGTLWAWGINNKGQLGTGLPVGCNAPVQVGNDGDWKAVSAGASFVAALKDDGSLW
                     TWGDNSMGQLGDGTLVDVYAPHRVGGEKDWAEVSAGSTHCVAVKKDGSLWAWGDSRLG
                     QLGEGLSAVPRATPVRIGGENDWVHVSAGWGHTLAIRKDGSLWAWGDNSHGQIGDGTT
                     AARPSPVMVSPASWKGISAGYQFSVGILHDGSLWAWGDNSYGQLADGTKTQSVEPVRE
                     VSGSPWSFASAGRQHAIAIRNDGTAWVWGANWYGQLGDGTMTDSPVPLGLTF"
     gene            complement(2643..>4434)
                     /locus_tag="PLT69_05320"
     CDS             complement(2643..>4434)
                     /locus_tag="PLT69_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ13894.1"
                     /translation="EPREIIGKRMEDFLTLESSSRMRRVLTYELGRSPDVRGMSGTFE
                     MQIMTKDGALKDIEVTTSWILDDESRPVGLQGSARDITMRKKAEEELKRSEERFRDLA
                     ELLPETVYEADLSGRITFVNKAGLEQFGYTEEDVRGGLTVEDIVHPSDKGRLRENFLQ
                     VKAGRMTGLNEYTVVRKDGTTFSGLARSSPVTRDGRVTGFRGVLVDITEKKNLEARLM
                     HAQRMEAIGTLAGGVAHDFNNLLMGILGNVSLLLAGVEEGNPAYERLKSMEEYVRRGS
                     ELTKQLLGFARGGKYEVKTTDMAGFVQRSAEMFGRTKKDIRMHVKAVEGLHPVEVDRG
                     QMDQVFLNLFVNAWQAMPEGGDIFISLENSVLGEDEVAPHGLRPGRYVKITVTDTGVG
                     MPPEILSRVFEPFFTTKERGRGTGLGLASVYGIVKNHGGFILVDSEKDVGTSFTIYLP
                     ASDKTPEKEEKKDEGVEKGSGTVLLVDDEDMILSVGRGMLERLGYTVIAAQGGREGVR
                     LFGENAGKIDLVILDMVMPEFSGRETFEAMRRQDPGVKVLLSSGYSLDGQARQLMDSG
                     CNGFIQKPFTMAELSKKISEILKEDRTQAA"
BASE COUNT          773 a         1486 c         1407 g          768 t
ORIGIN      
        1 cccatccgcg agggctacgg gctcaacgag accacgggta tcacccacat ctcggacgag
       61 gagaacttca agctcgggac cgtcggcagg tccctgcccg agtccgaggt gaagatcgcc
      121 gaggacggtg agatcctcat caggcacggg ggcgtgttca agggttactt caaggagccc
      181 gccgcaaccg ccgccgcgct cgagggcggc tggttccaca ccggggacgt gggagagatc
      241 gacgacgagg gctacctcaa gatcacggac cggaagaagg acctcatcat cacggccgga
      301 ggcaagaacg tagcccccca gtacatcgag aacctcctca agttctcgcc ctacatcaac
      361 gacgccgtgg tcatcggcga caggcgcaag tacctcacgg ccctcatcgt catcgacgag
      421 gagaacgtga tgaagtacgc ccaggacaac aaggtgcagt acaccacgtt cgcgagcctc
      481 acgagggccg aggagatcgt gaagctcatc gaggcagagg tggccaaggt gaacaggcag
      541 ctggcgcgcg tggagaacat caggaagttc cgcctgctgg acaagaagct ctacacggaa
      601 gacggcgagg tcacccccac catgaaggtc aagagggcgg ccatagcgag gcagtacaag
      661 gacctcatcg aggagatgta cgcggaagac tgagaggccc tgccccggca acgggtccgg
      721 cccgagactg ccggagggtt ccctctcgtc cgggagcacc gaagggcgcc tgttgcgccc
      781 cgaagacagg ggcgccgggc ctttctgtcc ggaccccgaa gccgggctca cccggcatgc
      841 cccactccac acccaaggcg tcccaggcaa gcttaccccg caggagactt ccccggggca
      901 gcgacgcctc ggcaagggtc tccctctccg agagacctgg tccatgcagc tggtgggcac
      961 tcgcgccgca ggcctttcgc gtgcgagacg tctccccccc ggcgcggaag ccatgcctgg
     1021 gcctgcaggg tctcccaccc ttatccgcca ggccgaacgt ggaaccctgg cttcccttcc
     1081 ggggaaatct ttttctctcg acttgttgcc acacagagcg gccgtggagc taaccccctg
     1141 aaaccccacg acgcaggccg ttgcatgctt attgctaaga gacgctcatt gcaatgcagt
     1201 ggcgttggaa tcggggaggg gcgctcacgg gggctcccct tcctcttatc gtgtttgctc
     1261 ctgaaagaga ccctggggaa tcggaggctg gatgatcatt cgaaggtacg ccgttttcgt
     1321 cctggccgcg gccgttctcg cactcttcgc agctgcatgc agcaatgacg actccggcgg
     1381 cccagcggcc ccggaagaac tcaccacgtg catctcggtg agcgcggggg aaaaccacac
     1441 gttggcggtg gactccgacg gcaggctctg ggcatggggc cacaacaggt acggccagct
     1501 gggcgacggg acgtgggagg actcgaacgt gcccgtgccc gtcggccacg acacggattg
     1561 gaagtcggtg agcgccggga accacttctc ggttgccgtc aagcacaacg gcaccctgtg
     1621 ggcctggggc atcaacaaca aaggccagct cggcacaggg ctgccggtgg ggtgcaacgc
     1681 cccggtgcag gtaggaaacg acggcgactg gaaggcagtg agcgccgggg cctcgttcgt
     1741 cgcggccctc aaggacgacg gctcgctctg gacgtggggc gacaactcca tgggccagct
     1801 cggtgacggc accctcgtgg acgtctacgc ccctcatcgt gtgggcggcg agaaggactg
     1861 ggccgaggtg agcgccggct ccacccactg cgtcgccgtg aagaaggacg gctcgctctg
     1921 ggcttggggc gactcaaggc tgggccagct cggtgaaggg ctgagcgccg tcccccgggc
     1981 aacccccgtg cgcatcggcg gcgagaacga ctgggttcac gtgagcgccg gctgggggca
     2041 caccctcgcg atcaggaagg acggctcgct ctgggcctgg ggagacaact cccacggcca
     2101 gatcggcgac ggcaccaccg cggccaggcc gtccccggtg atggtctcgc cggcgtcatg
     2161 gaagggcatc agcgcgggct accagttcag cgtgggcatc ttgcacgacg ggtccctgtg
     2221 ggcctggggc gacaactcct acggccagct ggctgacggc accaagaccc agagcgtgga
     2281 acccgtccgg gaggtctccg gctctccctg gtcgttcgcc tcggccggca ggcagcacgc
     2341 gatcgccata aggaacgacg gtacggcatg ggtctggggg gcaaactggt acggacagct
     2401 gggtgacggc accatgacgg actctcctgt ccccctgggt ctcacgttct gacaggaccc
     2461 tgacccggac gaagccgcct ggccggcacg caccctgccg ggatgcgcga tggcactcgt
     2521 gcgtgcaagg gtttcccgca aggaaccgat gggcagaagg ccgtccagac aaccatgacc
     2581 ctgtcgccgg gctcggcaga cctacgtgag ggttgcatga gccccggcgg tttctcaagg
     2641 tgtcaagcgg cctgggtccg atcctctttc aggatctccg agatcttctt cgacagctcc
     2701 gccatggtga agggcttctg tatgaacccg ttgcaccccg agtccatgag ttgccgcgcc
     2761 tgcccgtcga ggctgtaccc gctggacagg agcaccttca cgcccgggtc ctgcctgcgc
     2821 atggcctcga aggtctccct gccggaaaac tcgggcatga ccatgtccag gatcacgagg
     2881 tcgatctttc ctgcgttctc cccgaacagc ctgacgccct ccctgccccc ctgcgccgcg
     2941 atgaccgtgt atccgaggcg ttccagcatg cccctgccca cgctgaggat catatcctcg
     3001 tcgtccacga ggaggaccgt tccgctgccc ttctcgacgc cctcgtcttt cttctcttcc
     3061 ttttccgggg tcttgtccga ggcgggaagg tagatcgtga acgaggtccc cacgtccttc
     3121 tcgctgtcca cgaggatgaa gccgccgtgg ttcttcacga taccgtagac cgaggcgagg
     3181 ccgagacccg ttccccggcc gcgctccttg gtcgtgaaga agggctcgaa cacccgggag
     3241 aggatctcgg gtggcatgcc cacccccgtg tccgtcaccg tgatcttgac gtacctgccg
     3301 ggcctcagcc cgtggggcgc gacctcatcc tcgccaagca ccgagttctc cagggagatg
     3361 aagatgtcgc cgccctcggg catggcctgc caggcgttca cgaagaggtt cagaaagacc
     3421 tggtccatct gcccgcggtc cacctcgaca ggatggaggc cttctacggc cttgacgtgc
     3481 atgcggatgt ccttcttggt cctcccgaac atctcggcgc tcctctgtac gaacccggcc
     3541 atgtccgtgg tcttgacctc gtacttgccg cccctggcga accccaggag ctgcttggtg
     3601 agctccgatc ccctccgcac gtactcctcc atgctcttga gcctctcgta ggccgggttc
     3661 ccttcctcga cgcccgcaag cagcagggag acgttgccga ggatgcccat gaggaggttg
     3721 ttgaagtcgt gcgccacacc gcccgcgagg gtaccgatgg cctccatgcg ctgggcgtgc
     3781 atgagcctcg cctcgaggtt cttcttctcg gtgatgtcga cgagcacccc ccggaacccg
     3841 gtcacgcgcc cgtctctcgt gacgggagag gaacgggcga gcccggagaa ggtggtcccg
     3901 tccttgcgga ccacggtgta ctcgttcagg ccggtcatcc tgcctgcctt gacctgaagg
     3961 aagttctccc tgagccttcc cttgtccgac gggtgcacga tgtcctccac ggtgagtccg
     4021 cctcggacat cctcctcggt atacccgaac tgttcgagcc ccgccttgtt cacgaacgtg
     4081 atcctgcccg acaggtcggc ctcgtacacg gtctcgggaa ggagctcggc gaggtccctg
     4141 aaccgctcct cgctcctttt gagttcctct tcggccttct tgcgcatggt gatgtcacgc
     4201 gcgctcccct gcaggcctac cggcctggac tcgtcgtcca ggatccacga ggtggtcacc
     4261 tcgatgtcct tgagagcccc atccttcgtc atgatctgca tttcgaaggt cccggacatg
     4321 ccccgcacgt cgggactccg gccgagctcg tacgtgagca cgcggcgcat cctcgaggag
     4381 gattccaggg tgaggaaatc ctccatgcgt ttcccgatga tctcgcgggg ttcg
//
LOCUS       DAOSCR010000164         4416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12467_length_4416_cov_3.791103, whole genome shotgun sequence.
ACCESSION   DAOSCR010000164 DAOSCR010000000
VERSION     DAOSCR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12467_length_4416_cov_3.791103"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /gene="pnp"
                     /locus_tag="PLT69_05325"
     CDS             <1..1401
                     /gene="pnp"
                     /locus_tag="PLT69_05325"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ13895.1"
                     /translation="HEPVRNDARLHQIQEMASGRIEQIFKGAHTKQARNALIDQLFLE
                     VMKTFSDDTEERQGFVKAAFEEIEKKTIRSLMKQSKTRVDGRAFDDIRPIECKVGILP
                     RTHGSALFTRGETQALATTTLGTPRDEQRVETLLGETSKSFMLHYSFPPFSVGEVRML
                     RGPSRREIGHGNLAERALSKVLPEEGTFPYTIRLVSEILESNGSSSMATVCGGSLSLM
                     DAGIPVKKAVAGIAMGLLKEDDEYMILSDILGDEDHLGDMDFKVAGTKDGITALQMDI
                     KISGIPEDVLRRALNKAREGRLFILEKMNEAISAPRPSISPYAPRIFTLYINPEKIRD
                     VIGPGGKVIKDITSRTDTKIEIDDSGRVDIFSPDEDHAKMAVDMIQMLTKELEVGRVY
                     SGKVVKIMDFGAFVDFPSGESGLVHISQLSNERVKNVRDVVKEGDEIIVKVIGIDSKT
                     GKIRLSRKDALSETKR"
     gene            1411..2718
                     /locus_tag="PLT69_05330"
     CDS             1411..2718
                     /locus_tag="PLT69_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ13896.1"
                     /translation="MRNSTVLPNGVRILSERLEGVRSVSLGVWVNAGSRNDPPDGTGI
                     AHFTEHMLFKGTKRRSAIQIAKEIDALGGHLNAQTAKEYTVYYTKVLDEHISKACDIL
                     FDLFLNSEVSQEELEKEKKVVLQEIRMTEDTPDDYITDLFAETFFHGTPLGTSILGTT
                     EHVSSFNRSSVLNFMEAAYAPGSIIIAGAGSIDHDNLVSMASPLFENIPPRHGRHDRD
                     ASCAPAGLKKVVYRDIEQVHFTLGTRGSAMSDEKRWVFIVLNTILGASMSSMLFQEVR
                     EKRGLVYSIYSYLNAYRDCGVLAVYAACTPDHVNETLDVIREQLDRLAGGKIDDIRIE
                     DIKAQIKGNLLLARESSVNRMSSMAKNEIYYGREVTVEEALEKIENVSLDDVASLARE
                     IFDEQKGFTFVALGRVRPEDVAWPDVPAGTTTGDPASRVRVPG"
     gene            complement(2695..2967)
                     /locus_tag="PLT69_05335"
     CDS             complement(2695..2967)
                     /locus_tag="PLT69_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13897.1"
                     /translation="MSICEATDDALVCTFPSCRIESSLMDLISMPATIRIDSTSIEGT
                     LSYYTIEDSTCRVGISVDKRHRSAWRRICAEKIAEHCLSSTRHAHA"
     gene            3111..3845
                     /gene="truA"
                     /locus_tag="PLT69_05340"
     CDS             3111..3845
                     /gene="truA"
                     /locus_tag="PLT69_05340"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOJ13898.1"
                     /translation="MRNIKCVIAYDGTDFLGWQTQASGRTVQGVLEDALATILGERVR
                     VHAAGRTDAGVHALGQVVNFKTVSSIPVAGFLRGINSMLAHDVTVLSAEEAASDFHAR
                     YSAKLKSYVYVIDTAPQKNPFLRRYTHHLGAPLDVEAMGRAAGILCGEHDFSSFMGAG
                     SCVKSHVRTVVSSRVESRGSLVYLWVQGGGFLRHMVRNIAGTLILVGRGRLDSSGVER
                     ILEGRDRSLAGPTAPPQGLYLVSVTY"
     gene            3864..>4416
                     /locus_tag="PLT69_05345"
     CDS             3864..>4416
                     /locus_tag="PLT69_05345"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ13899.1"
                     /translation="MQRVLITGGCGFIGSNLVRYILRNRPDWHVVNVDLLTYAGNLEN
                     TEGLEQAYGSRYSFARGDISDERFIEELFSRNKFDLVLNLAAESHVDRSIEGAGVFVK
                     TNVLGVQVLLDAALRHGTGRFLQVSTDEVYGSLGAGGSFHEGLPLAPNSPYSATKAAA
                     DRLVKAYVHTHGMDAVITRWSNNY"
BASE COUNT          960 a         1301 c         1377 g          778 t
ORIGIN      
        1 cacgagccgg tccgcaacga cgcccggctc catcagatcc aggagatggc ctccggccgg
       61 atcgagcaga tcttcaaggg ggcgcatacc aagcaggcca ggaacgccct catcgaccag
      121 ctctttttgg aggtgatgaa gaccttctcc gacgacaccg aggagaggca ggggttcgtc
      181 aaggccgcct tcgaggagat agagaaaaag accatcaggt cgttgatgaa gcaatccaag
      241 acaagggtgg atggaagggc gttcgacgat atccggccca tagaatgcaa ggtgggcatc
      301 ctccccagga cccacgggtc cgcgctcttc accaggggcg aaacccaggc gctcgcaacc
      361 acgacgctgg gcaccccgag ggatgaacag cgcgtcgaga ccttgctcgg ggagacgagc
      421 aagtcattca tgctccacta ctcgttcccg ccgttttcgg tgggcgaggt gaggatgctc
      481 agggggccga gcaggcgaga gataggccat ggcaatctcg cggaaagggc cctgagcaag
      541 gtcctccccg aggaaggcac cttcccatac accatccgcc tcgtctcgga gatcctcgaa
      601 tcgaacggct cgtcttccat ggccacggtc tgcggaggct cgctctccct gatggatgcg
      661 ggcatacccg tgaagaaggc ggttgcgggc atcgccatgg ggctcctcaa agaagacgac
      721 gagtacatga tcctctccga tatcttgggc gacgaggatc acttgggcga catggacttc
      781 aaggtcgcgg ggacgaaaga cggcatcacc gccctccaga tggacatcaa gatctcggga
      841 atccccgagg atgtcctcag gcgggcgctc aacaaggcga gggaggggag gctcttcatc
      901 ctcgagaaga tgaacgaggc cataagtgcg ccgaggccct ccatatcccc gtatgccccc
      961 cggatcttca ccctgtacat caatcccgag aagatccggg acgtgatcgg cccgggcgga
     1021 aaggtgatca aggacatcac ctcgcggacg gacaccaaga tcgagatcga cgactcgggc
     1081 agggtggata tcttctcccc tgacgaagac catgcgaaga tggccgtgga catgatccag
     1141 atgctcacca aggagctcga ggtgggccga gtctacagcg ggaaggtcgt caagatcatg
     1201 gacttcggcg cgttcgtgga cttcccttcc ggagaatccg ggctcgttca catctcccag
     1261 ctgtccaacg agagggtcaa gaacgtgagg gatgtcgtga aggagggaga cgagatcatc
     1321 gtgaaggtca tcggcattga cagcaagacc ggcaagatcc gtctcagccg caaggacgcg
     1381 ctcagcgaga ccaagcgata accctcccgt atgcgtaact ccacagttct ccccaatggg
     1441 gttcggattc tttccgaacg gctggaaggt gtgcgttcgg tttccctcgg ggtctgggtg
     1501 aacgcaggat cccgaaacga cccgccggat ggcaccggta tcgcccattt caccgagcac
     1561 atgctcttca agggaacgaa aaggcgaagc gccatccaga tagccaagga gatcgacgcg
     1621 ctcggcgggc acctcaacgc gcagacggcc aaggaatata ccgtctacta cacgaaggtc
     1681 ctcgacgaac acatctccaa ggcgtgcgac atcctcttcg acctcttcct gaactcggag
     1741 gtgagccaag aggaactcga gaaggaaaag aaggtcgttc tccaggagat ccgcatgacc
     1801 gaggacaccc cggatgacta catcacggac ctgttcgccg agaccttttt ccacggcacg
     1861 cctctcggca cgtccatact ggggacaacc gagcacgtca gctccttcaa ccggtcgagc
     1921 gtcttgaact tcatggaggc ggcctacgct ccaggctcca ttatcatagc gggcgccggg
     1981 agcatcgatc acgacaacct cgtttccatg gcctcgcccc tcttcgagaa catcccaccc
     2041 aggcacggca ggcacgacag ggacgcctca tgcgcgcctg ccgggttgaa aaaggtcgtc
     2101 taccgcgaca tcgaacaggt gcacttcacc ctcggcacga gagggtctgc gatgagcgac
     2161 gagaagcgat gggtcttcat cgtcctgaat accatactcg gcgcctccat gagctcgatg
     2221 ctcttccagg aggttcgtga aaagcgcgga ctcgtctatt ccatttattc ctacctcaac
     2281 gcctatcgtg actgcggggt gctcgccgtt tacgcggcat gcacgcccga tcacgtgaac
     2341 gaaaccctgg acgtcatacg agaacagctc gacaggctcg ccggcgggaa gatcgacgat
     2401 atacgcatcg aggacatcaa ggcccagata aagggaaacc tgctcctcgc cagggagagc
     2461 tcggtgaaca ggatgtcgtc catggcgaag aacgagatct attacggtcg tgaggtcacc
     2521 gtggaagagg cgctcgagaa gatcgagaac gtctcgctcg acgacgtggc gagccttgcc
     2581 cgggagatct tcgacgagca gaagggtttc acgttcgtgg cgctgggaag ggtcaggccc
     2641 gaggatgtcg cgtggcccga cgtcccggcc ggcaccacga caggggatcc cgcctcacgc
     2701 gtgcgcgtgc ctggttgaag aaagacagtg ctcggctatc ttctccgcgc agatcctgcg
     2761 ccaggcggag cggtgccgct tgtccaccga aatccccacc ctgcaggtgg aatcctctat
     2821 ggtgtagtag gacagggtcc cctcgatgga tgtggaatcg atgcgtatgg tggcaggcat
     2881 ggagatcagg tccatgaggc tggactcgat acggcacgag ggaaaggtac agaccagcgc
     2941 gtcgtcggta gcttcacaga ttgacacgag aagcgacgtt ttgccgatgg aacacgaggc
     3001 actcatggaa ggaatctgtc tttcgttgtt cagccacctg aagatgccca tatgggagct
     3061 cccccgcgtc gtggtataca ctaccctttt cggcaggcaa gagggggtac atgagaaata
     3121 tcaaatgcgt aatcgcatat gacggcacgg acttcctcgg ttggcagacg caggcctcgg
     3181 ggaggacggt gcagggggtg ttggaggacg ccctcgccac gatcctgggg gaacgggtca
     3241 gggtgcacgc ggcgggcaga acggatgcgg gcgtgcatgc actgggacag gtggtgaact
     3301 tcaagaccgt ctcctccatc ccggtcgccg ggttcctcag gggaatcaac tcgatgctgg
     3361 cccatgacgt gaccgtcctg tccgccgaag aggcggcgtc cgacttccac gccaggtact
     3421 cggcgaagct caagtcgtac gtctacgtca tcgacaccgc accgcagaag aacccgtttc
     3481 tcaggcgata cacccaccac ctcggcgccc cccttgacgt ggaagcgatg ggccgcgccg
     3541 ccgggatcct gtgcggagag catgatttca gctcgttcat gggcgcgggg tcttgcgtga
     3601 agtcccacgt gcggacggtc gtctcctcga gagtggagtc gaggggatcc ctggtgtatc
     3661 tctgggtgca gggtggaggt ttcctgcgcc atatggtaag gaacatcgca ggcacgctca
     3721 ttctcgtagg ccggggaagg ctcgattcct cgggcgtgga aaggatcctt gagggaaggg
     3781 atcggtctct cgccggccct accgctcccc cccaaggtct gtatttggtg agcgtgacat
     3841 attgagccca gaggggtgcc tcgatgcaac gggtcctcat aacaggcggg tgcggcttta
     3901 tcggctccaa cctcgtacgg tacatcctcc gaaacaggcc agactggcac gtggtgaacg
     3961 tggatctcct gacgtacgcg ggaaacctcg agaacaccga aggactggaa caggcctacg
     4021 gttcgcggta ttccttcgcc aggggtgaca tctcggacga gaggttcatc gaggaactgt
     4081 tcagtcgcaa caagttcgac ctcgtcttga acctcgcggc cgagagccac gtggacagaa
     4141 gcatagaggg cgcaggggtc ttcgtgaaga ccaacgtcct gggtgtccag gtcctgctcg
     4201 atgccgcact cagacacggc acggggaggt tcctgcaggt gtccacggac gaggtctacg
     4261 gcagcctggg ggccggcggg agttttcacg aggggcttcc cctggccccc aacagcccct
     4321 actccgcgac caaggccgcg gcggaccgcc tcgtgaaggc gtacgtacat acgcacggca
     4381 tggatgcggt gatcacaagg tggtccaaca attacg
//
LOCUS       DAOSCR010000165         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12472_length_4414_cov_2.662996, whole genome shotgun sequence.
ACCESSION   DAOSCR010000165 DAOSCR010000000
VERSION     DAOSCR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12472_length_4414_cov_2.662996"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLT69_05350"
     CDS             complement(<1..914)
                     /locus_tag="PLT69_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ13900.1"
                     /translation="MATREIRTRRHTIELKETKWDIFIWLGSFVVLGFVVVFAMYSFY
                     GVEREMAAQFNREQGLLAQQTAMGIEQYMHDITNILELTSHMDRVSQGDPEAIAPALK
                     NAYSSLKNKVVFLFWEDADGVMRIHYPRDVLPGLEGRDFSFRTYFRVARDLGVPFVSD
                     IIMVGGEQYSDIPGRFETFVIAYPIKDQKGVFKGVLGCAIDLSNITVHYVAPIRPSEA
                     GYVWLLDESGMVLYHPDPKWIGMNLKDIIIELKRQGVKISGVDEIRQAMEIKNDGMFE
                     FTFPNYPDKKPVRKLLAFSSVHFLNRRW"
     gene            complement(922..2199)
                     /locus_tag="PLT69_05355"
     CDS             complement(922..2199)
                     /locus_tag="PLT69_05355"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOJ13901.1"
                     /translation="MRIESDLGGVDLDLVERIMRGEGLAEVPPSEVRPAAVVPASFSS
                     SPSAAQCVLAGRRLLEQGKVAALLVAGGQASRLGVESPKGCVEVTPLTGKTLFRVFAE
                     QILALGRRFGRPVPWFIMTSPVNHAQTVEYFTANDFLGLDPRMVFFFPQGVLPSLTGE
                     GKFILAPEGGLYLNPDGHGGVFRALLTSGMFDAMRDLGIEEIFTFQVDNPLVKVCDPL
                     FVGLHSMQGACMSSKVVRKAGFDEKVGVMVEYGGRTRLLEYSDMDDAIRYATDENGEM
                     LHWAGSIAIHLMRLDFAREVASRSHALPFHRAVKKIPALAPDGTTVTVEGIKFETFIF
                     DALAHARTSVTLEVTRSEEFAPVKNLTGTDSLETSRVLQSKRHVSWLRAIGVDVRDGV
                     TVEISPLFALDREELASRRAEIGDVITRDTYLG"
     gene            complement(2219..3610)
                     /locus_tag="PLT69_05360"
     CDS             complement(2219..3610)
                     /locus_tag="PLT69_05360"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOJ13902.1"
                     /translation="MDLEEPTYTYRHEPGLLERPHGGFLLAVSFSCYTYAMKGQTPPL
                     AFRRRPVRIEDLMGQEEASALLEDFSRGAVRSAILWGPPGSGKTSVVSIIEHLFPEDF
                     HAIQAVSSGVQEIRAVSKRSPSGKTPIVFIDEIHRFTRVQQDSLLPIVESAEVILVGA
                     TTENPSFALTPPLVSRTQVVLMKSLDVDTLVTILRSALEQDEILLSMNKQVADECIAA
                     IARASDGDARAALNLLELAMTRIDKEVIEPGDIERLLKRPLYHDSSGEYHYDLISAFH
                     KSVRAGDADASVYWLGRMLEAGEDRLFIVRRMIRIASEDIGLADPNALRMAVAARDAF
                     TFVGSPEGELALYEVAIYLACAPKSNAVYLTEHRVRDLIRKTGTPEVPLHLRNAPTKL
                     MRELGYGRGYIYPHDDYEGALDLAYMPESLGGLELYKPKDSGVEKRIKEILDARRKAQ
                     KAGSGSHRRHPRG"
BASE COUNT          725 a         1416 c         1426 g          847 t
ORIGIN      
        1 atccaccgcc tgttgaggaa gtggaccgac gagaacgcga gcagcttcct cacgggtttc
       61 ttgtccgggt agttggggaa ggtgaactcg aacatcccgt cgttctttat ctccatggcc
      121 tgcctgatct cgtccacccc cgagatcttg accccctggc gcttgagctc gatgatgatg
      181 tccttgaggt tcatgcctat ccacttgggg tcgggatggt agagcaccat gccgctctcg
      241 tcgagcaacc agacgtagcc cgcctcggaa ggccgtatgg gggccacgta gtgcacggtg
      301 atgttggaga ggtcgatggc gcagcccagc acacccttga agacgccttt ttggtccttg
      361 atggggtacg cgatgacgaa ggtctcgaac ctgcccggga tgtccgagta ctgctcgcct
      421 cccaccatga tgatgtcgga gacgaagggc acgccgaggt ccctcgccac cctgaagtag
      481 gtgcggaaac tgaaatccct gccctcgagc cctggcagca cgtccctcgg gtagtggatg
      541 cgcatgaccc cgtcggcgtc ctcccagaag aggaagacca ccttgttctt cagggaggag
      601 tacgcgttct tcaaggccgg ggcgatggcc tccgggtctc cctggctcac gcggtccatg
      661 tggctcgtga gctcgaggat gttcgtgatg tcgtgcatgt actgctcgat gcccatggcg
      721 gtctgctgtg cgaggagacc ctgttcgcgg ttgaactggg ccgccatctc gcgttccacc
      781 ccgtagaagg agtacatggc gaagaccacc acgaacccga gcaccacgaa gctcccgagc
      841 catatgaaga tgtcccactt cgtctccttg agctcgatgg tatggcgtct ggtccgtatc
      901 tctctcgttg ccacgtcgac ctcaccccag gtaggtgtcc cgcgtgatca cgtcaccgat
      961 ctcggcccgc ctcgaggcga gctcttccct gtcgagcgcg aacagcgggc tgatctccac
     1021 ggtgaccccg tctctgacat ccacgcctat tgcacgcagc cacgagacgt gccttttgct
     1081 ctggaggacc ctcgatgtct cgagggagtc cgtcccggtg agattcttga cgggcgcgaa
     1141 ctcctcggac cgcgtcacct cgagggtgac gctcgtccgg gcgtgcgcca gggcgtcgaa
     1201 gatgaaggtc tcgaacttga tcccctcgac cgtgacggtc gtcccgtcgg gcgcgagggc
     1261 ggggatcttc ttcacggccc tgtggaaggg cagggcatgg gatctcgacg ccacctcccg
     1321 ggcgaagtcc agcctcatga ggtggatcgc gatcgacccc gcccagtgga gcatctcccc
     1381 gttttcgtcc gtggcgtacc tgatggcgtc gtccatgtcg ctgtactcga ggagcctcgt
     1441 cctccctccg tactcgacca tgaccccgac cttctcgtcg aagcccgcct ttctgacgac
     1501 cttggacgac atgcaggcgc cctgcatgct gtggagcccc acgaacagcg ggtcgcacac
     1561 cttgacgagg gggttgtcca cctggaaggt gaagatctcc tcgataccca gatcgcgcat
     1621 cgcgtcgaac atgccggagg tcaggagggc cctgaacacg ccgccgtggc cgtccgggtt
     1681 caggtagagg cccccctccg gtgccaggat gaacttcccc tctccggtca gcgagggcag
     1741 cactccctgg gggaagaaga acaccatccg tgggtcaagc ccgaggaagt cgttcgccgt
     1801 gaagtattcc accgtctgtg catggttgac cggcgaggtc atgatgaacc agggcacggg
     1861 tctcccgaag cgcctgccga gcgcgaggat ctgctctgcg aacaccctga acagggtctt
     1921 tcccgtaagc ggggtgactt ccacgcaccc cttcggtgac tccaccccga gcctcgaggc
     1981 ctggcccccg gcgacgagga gggccgcgac cttgccctgt tcgaggagcc tccttcctgc
     2041 gagaacgcac tgcgccgcag acgggctgga cgagaatgac gccggcacca cggcggccgg
     2101 cctcacctcc gaaggcggca cctcggccaa cccttccccc ctcatgatcc tctccacgag
     2161 atcgaggtcc acccccccga ggtccgattc gatgcgcatg gcctcatgcc gccgagactc
     2221 atcctcgagg atgtctgcga tgtgatcctg acccagcctt ctgagccttt ctgcgagcat
     2281 cgagtatctc cttgatcctc ttttcgacgc cggagtcctt cggcttgtac agctcgaggc
     2341 cgccgaggct ctcgggcatg tatgcgaggt cgagcgcccc ctcgtagtcg tcgtgcggat
     2401 agatgtaacc ccttccgtat ccgagctccc gcatgagctt ggtgggtgcg ttgcggagat
     2461 ggagcggcac ctcgggagtc ccggtcttcc tgatgaggtc cctcacgcgg tgctcggtga
     2521 gatacaccgc gttgctcttg ggtgcacagg cgaggtatat ggccacctcg tagagggcga
     2581 gttccccctc ggggctcccc acgaaggtga atgcgtcccg cgccgcaacg gccatgcgca
     2641 aggcgttcgg gtccgcaagg cctatgtcct cgctcgcgat cctgatcatc ctgcggacga
     2701 tgaagagcct gtcctcgcct gcctccagca ttctgcccag ccagtagacg ctcgcgtccg
     2761 cgtcgcccgc acgcacgctc ttgtggaagg cggagatgag gtcgtagtgg tactcgcccg
     2821 agctgtcgtg gtagaggggg cgtttcaaga gcctctcgat gtccccaggc tcgatgacct
     2881 ccttgtcgat ccgcgtcatg gccagctcga gcaggttcag ggcggctcgc gcgtccccgt
     2941 cgctggcccg ggcgatcgcg gcgatgcact cgtcggccac ctgcttgttc atggatagca
     3001 ggatctcgtc ctgttcgagg gcgctcctga ggatcgtgac gagggtgtcc acgtccagcg
     3061 acttcatgag gaccacctgg gtgcgtgaga cgagcggcgg ggtgagggcg aaggaggggt
     3121 tctccgttgt cgcacccacg aggatcacct cggcgctctc cacgatcggg agcagcgagt
     3181 cctgctgaac cctcgtgaac cggtgtatct cgtcgatgaa gacgatcggg gtcttgcccg
     3241 agggggatct tttcgacacg gcacggatct cctggacacc ggaagagacg gcctggatgg
     3301 cgtggaagtc ctcggggaac aggtgttcga tgatggagac cacgctcgtc tttccgctgc
     3361 ccggggggcc ccagaggatg gccgagcgga cagcccccct cgagaagtcc tcgaggagcg
     3421 cggatgcctc ttcctggccc atgaggtctt cgatgcgcac gggcctccgc ctgaaggcga
     3481 gcggcggggt ctgtcccttc atggcgtagg tatagcatga aaaggagacc gcgagaagga
     3541 atccaccatg gggcctctca agcaatcctg gttcgtgtcg ataggtatac gtaggttctt
     3601 caagatccat tccccgtaga acctcctcat acgttgcagg tcagccatga cctgcttttt
     3661 tttgttcatg cggcctccgg cgcctgggcc gatcgtccgt ggggaaaggg gtgcgccgtg
     3721 agctcaggtg cacgaggggc gtcaagacgg tcagcaacga gtggccgccc cgcctggggc
     3781 tcggcctcgt cttcggacgc ggcggccgcc aggcggcgcc gagggtgaag cgtgcaccgg
     3841 ttcaccagac aaggggcatg ccggcgcctg tctcaagccc tctccctgca cgccgcaccc
     3901 cgttgtccgt ctgtggggtg cgagccttgt gggcgtgcgg cgcgacgtcg aaagggcggt
     3961 gtgcgtggca aggcgcgcct ggtaaccctg gccctcacga tgcccaaatc cgctcgaagg
     4021 tttgaacaag aagtcaccgc gtcgatgacg cctggtctgc tccaggccgc agcggtacgg
     4081 ctggggtact gcaagaaggc accgcgccga tgacgcccct tgggctgcgc ccaggggttt
     4141 tatcgggaag gggacgagcc tcgcgtggga taccctgcct gcgggacagg cgtcagggat
     4201 cgcggcgcca tcgcccggcg ccgtagcgag agaggcggtc atgtcctggt cggaacaggg
     4261 gcggtgatgc catcggcgtc gaggcgtcgt cgggaccctg tgtgttcccg gtaaaggggc
     4321 gtgtccaccc ggcaaaggtc cctgcgtgta cgcccgagac cgcgggtgcc tggcatggct
     4381 caccttggct cgtgcagggg atgtgcatgc cccg
//
LOCUS       DAOSCR010000166         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12506_length_4404_cov_3.456427, whole genome shotgun sequence.
ACCESSION   DAOSCR010000166 DAOSCR010000000
VERSION     DAOSCR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12506_length_4404_cov_3.456427"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLT69_05365"
     CDS             complement(<1..412)
                     /locus_tag="PLT69_05365"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOJ13903.1"
                     /translation="MDLALIEKAADLLVRARHTVVLTGAGISTESGVPDFRSPGGIWE
                     RYDPSLFFLSRFLSEPDVVWKCMIEMHTSGEFHLWEAKPNDGHLALAELERMGIVKVV
                     ITQNVDCLHQKAGSRNVIEFHGNMLTFRCLSCGKT"
     gene            complement(474..3266)
                     /locus_tag="PLT69_05370"
     CDS             complement(474..3266)
                     /locus_tag="PLT69_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ13904.1"
                     /translation="MNRPQPRLILCLAVSLTFSPLARGAAIAGGLEGVSLGPEAFQAR
                     PGELINVLFEVTNTTDNDITAATDAILPEGWKVVNRPGPLRLAPSARAVFIFSVVVPS
                     AARAATYEAAFRVFAQEAPSMSYRHGITVTVQEVQGLTVNLVDAPRTVVSGGLYAASF
                     LVKNTGNDTVVLALSCSSLGGIPAVVDVDRVELAPGQSHVARVTARIPEDIPRGFTHR
                     LFLEARSIGPRPLAAQGWADVEVIRSGTPFLERFTTIPATLTLRQATQHGGKTASSLQ
                     AALEAQGPVDEAGKHHVELVLVGPDTTDETTFGTQDQYLFRAYSETYDVVAGDSQFSL
                     SPLTEQYLAARGAGLGLSLGGLSVKGFSARSRWQEPDVTETGGSLGYRFGSSLDVTLN
                     ALGKSGEDEDAKVASAQVSIRPLPWMDMDIEGAQGNGGDAKGEAVRASVSCAGPPGSA
                     RLSYLDADPDFPGYHKDKRLSSLDLVSNVSKPLRASLSFNREERNLKREPDLAQAPVD
                     TRTRLGVDYLADDKTTYSLAVSGRRLDDRLPSPLTDFTEVTMRAGVRKQLPSASCNVF
                     AETGGRRDETDGEVSPLWQVALTAYGTRDRDLSFGLHARVASKVNIDPANARTVSAGA
                     SVACSFSRETRIELSGQVDADRSDASRNKYLLEASVKKDLWQDSTLTARASRTLYTGH
                     TDTKNETALLIEYAHEFGLPVARKAGTSTVSGIVRDASSSKPLGGVILRLDGRSAATD
                     PEGRFSFLGLTPGTAYLGVDVSGLDPTSLPDCQNPLPLTLEAGRVIPVEILITKKATL
                     RGRIEIYKAFKGERALPTVLEGELLPRQEGAIELRPAAPLANALVELRGTGGTVRALT
                     DAHGLFTFPELRPGTWTLAVSREAIPEDHVLEPAELTFELAPGDAREVALKVLPKKRV
                     IRFMQEGGRVIQEEAR"
     gene            complement(3263..4093)
                     /locus_tag="PLT69_05375"
     CDS             complement(3263..4093)
                     /locus_tag="PLT69_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13905.1"
                     /translation="MHDHRYRKTGVLILAIVLLVNAPSLYAAISVEGGLTHERTARPG
                     ETYRETITLRNQSQEPSEVKVYQTDYWFNHEGKMRYDDPGTLDRSNARWINVNPVRVT
                     VPPMDRAVVSYTVNVPKDDDRIGTYWSILMVEEIPPSSPEASGRERTKQPAVGIVQVM
                     RYAVQVITHIGEGGLREVSFLKTSIVKEGEQRLLKVDVENTGQVLVRPQVYTELFKDD
                     GTSMGRFPGERYRLYPKTSRRFTVDVSTVPPGTYRAVVIADCGEDAVFGITYTFVFER
                     "
     gene            complement(4107..>4404)
                     /locus_tag="PLT69_05380"
     CDS             complement(4107..>4404)
                     /locus_tag="PLT69_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13906.1"
                     /translation="VSGTLNKTDAWEVSVKRIDTGRNPALAILARRTSNGKGQGSVSG
                     GTNFVQVTETDSPFFTGNGNIKDIQVQLSLTNMSLSVQPASYMTTIQFTVIDTP"
BASE COUNT          690 a         1408 c         1452 g          854 t
ORIGIN      
        1 aggtcttccc gcaggagagg cacctgaagg tgagcatgtt cccgtggaac tcgatgacgt
       61 tcctgctgcc cgccttctgg tggaggcagt ccacgttctg ggtgatgacc accttcacga
      121 tgcccatgcg ctcgagctcg gcgagggcga ggtgtccgtc gttgggtttt gcctcccaca
      181 ggtgaaattc gccggaggtg tgcatctcga tcatgcactt ccacaccacg tccggttccg
      241 agaggaagcg cgagaggaag aacaggctcg ggtcgtaccg ttcccagatg ccgccgggag
      301 accggaaatc gggcaccccg gattccgtgg atatccccgc acccgtcagc accacggtgt
      361 gtcgtgcgcg tacaaggagg tccgcggcct tctcgatgag ggccaaatcc atgagatgag
      421 cattaccaga ggggtcgttc gaggtcaagg aactcgcacg ggcctcgagg cgctcaccgg
      481 gcctcttcct ggatgaccct gcctccttcc tgcatgaacc tgatcacgcg tttcttggga
      541 agaaccttga gcgccacttc cctggcgtct ccgggtgcga gctcgaaggt gagctctgca
      601 ggttcgagca cgtggtcctc ggggatcgcc tccctcgata cggccagcgt ccatgtcccc
      661 ggcctgagtt cagggaacgt gaacagcccg tgtgcatccg tcagggccct gaccgtcccg
      721 cctgtccccc tgagctcgac gagcgcgttt gcgaggggcg ctgcgggcct gagctcgatg
      781 gcgccctcct gccggggaag cagttcgccc tcgagcaccg tcggcagggc gcgctcccct
      841 ttgaatgcct tgtagatctc tatgcggccc ctgagggtgg cctttttcgt gatgaggatc
      901 tccacaggga tcacccggcc cgcctcgagc gtcaagggga gggggttctg gcagtcgggc
      961 aggctcgtgg gatcgagccc ggagacgtcc acgcccaggt atgccgtccc cggggtcagc
     1021 cctaagaagg aaaacctgcc ctctgggtcc gtggccgcgg acctgccgtc gagcctcagg
     1081 atcaccccgc cgagtggctt cgagctcgat gcgtccctga cgatccccga gaccgtggag
     1141 gtgccggcct tcctcgccac ggggaggccg aactcgtgcg catactcgat gagcagggcc
     1201 gtttcgttct tcgtgtccgt gtgcccggtg tacagggtgc gcgacgccct ggcggtgagg
     1261 gtcgagtctt gccacaggtc cttcttgacc gacgcctcga ggaggtactt gttccgcgag
     1321 gcatccgaac ggtcggcgtc cacctggccc gagagctcga tcctcgtctc ccgcgaaaac
     1381 gagcaggcca cggatgcgcc ggcgctcacg gtcctcgcgt tggcggggtc gatgttcacc
     1441 ttgctggcca ccctcgcatg gaggccgaag gagaggtccc tgtccctggt tccgtatgcg
     1501 gtcagggcga cctgccacag gggcgacacc tccccgtcgg tttcatcccg tctccctccc
     1561 gtctcggcaa agacgttgca ggaggccgat gggagctgtt tccttacacc cgccctcatc
     1621 gtcacctcgg tgaaatcggt caggggagag gggagcctgt catcgagccg ccttcctgac
     1681 acggccaggg agtacgtggt cttgtcgtcc gccaggtagt cgacgcccag ccgtgtgcgg
     1741 gtgtccaccg gggcctgtgc gaggtcgggt tcgcgtttca ggttcctctc ctccctgttg
     1801 aaggaaaggc tggccctcag aggcttcgag acgttcgaga ccaggtcgag ggaggaaagg
     1861 cgcttgtcct tgtggtaccc ggggaagtcg ggatccgcat ccaggtagga caggcgcgcg
     1921 ctgccgggcg ggccggcgca agacacggac gccctgacgg cttcaccctt ggcgtccccc
     1981 ccgttgcctt gggcgccctc gatatccatg tccatccacg gcaggggacg gatcgagacc
     2041 tgggcggatg ccacctttgc atcctcatcc tcgccgcttt tcccgagggc gttcagggtc
     2101 acatcaagcg aacttccgaa cctgtacccg agggagcccc cggtctcggt cacgtcgggc
     2161 tcctgccatc gcgacctggc ggagaagccc ttcacggaaa gacccccgag ggaaagcccc
     2221 aggcccgccc cccgtgccgc caggtactgt tcggtgaggg gggacaggga gaattgcgag
     2281 tccccggcga ccacgtcgta ggtctccgag tatgccctga acaggtattg atcctgcgtg
     2341 ccgaaggtgg tctcgtccgt ggtgtccggt ccgacgagca cgagctccac gtggtgcttc
     2401 cccgcctcgt cgaccgggcc ctgggcctcg agggcggcct gcaaggacga cgccgtcttc
     2461 ccgccgtgct gggtcgcctg cctcagggtc agggttgcgg gtatcgtggt gaacctttcc
     2521 aggaaaggcg tgccgctcct gatgacctcg acgtccgccc atccttgagc ggccaaaggc
     2581 ctggggccta tggagcgcgc ctccaggaag aggcggtgcg tgaacccccg agggatgtcc
     2641 tcggggatcc tcgccgtgac cctggccaca tgtgactggc cgggagccag ctccacgcgg
     2701 tccacgtcca caacagccgg gatgccgccg agacttgagc aggaaagggc gaggacgacc
     2761 gtgtcgtttc ccgtgttctt gacgaggaac gatgcggcgt acaagccgcc cgagacgacc
     2821 gtacgcggcg catccacgag attcacggtg aggccctgga cctcttgcac ggtgacggtt
     2881 atgccgtggc ggtacgacat cgagggcgcc tcttgtgcga acacgcggaa cgccgcctcg
     2941 taggtggcgg cccgagcggc ggaggggacc acgacgctga agatgaagac ggctctcgcc
     3001 gagggggcga gcctgagggg gccgggcctg ttcaccactt tccagccttc cgggaggatc
     3061 gcgtcggttg ctgccgtgat gtcgttgtcc gtggtgttcg tcacctcgaa gaggacgttg
     3121 atcagctccc cgggacgcgc ctggaaggcc tccggcccca gggagacgcc ctcgaggccg
     3181 cccgcgatgg cggctcctcg cgccaggggc gagaacgtga gtgacacggc caggcagagg
     3241 atgaggcgtg gctggggtct gttcaacgtt cgaacacgaa ggtgtaggtg atgccgaaga
     3301 cggcgtcttc cccgcagtct gcgatgacga ccgcacggta ggtccccggt gggacggtcg
     3361 agacgtccac cgtgaaacgc ctcgacgttt tcgggtacag gcggtatctc tctcccggga
     3421 acctgcccat cgaggtgccg tcgtccttga agagctccgt gtatacctgg ggtctcacga
     3481 ggacctgtcc cgtattttcc acgtccacct tgagaagcct ctgttccccc tctttcacga
     3541 tggaggtctt gaggaacgag acctctctca gtcctccctc ccctatgtgc gtgatgacct
     3601 gcaccgcgta ccgcatgacc tggacgatgc ccactgccgg ctgtttcgtc ctttcacgcc
     3661 ccgaggcctc gggggacgag ggcgggatct cttccaccat gaggatgctc cagtaggtcc
     3721 cgatcctgtc gtcatccttc gggacgttca ccgtgtagga aaccacggcc ctgtccatgg
     3781 gcggcaccgt gacgcgtacc gggttgacgt ttatccaccg cgcgttcgag cggtcgaggg
     3841 tcccggggtc gtcgtagcgc atctttccct cgtggttgaa ccagtagtcg gtctgataga
     3901 ccttcacctc agagggctct tgtgactggt tcctgagggt tatggtctcg cggtaggttt
     3961 ctcccggcct ggccgtccgc tcgtgggtca gcccgccttc cacggatatc gccgcataga
     4021 gcgagggagc gttcaccagg aggacgatcg cgaggatcag gactcccgtc ttgcggtacc
     4081 ggtggtcgtg cacggttgtt cctccttcat ggggtgtcga tgaccgtaaa ctggatcgtg
     4141 gtcatgtacg atgcaggctg gaccgaaagg gacatgttcg tgaggctgag ctggacttgg
     4201 atgtctttga tgttgccgtt ccccgtgaag aaggggctgt ccgtctcggt gacctggacg
     4261 aagttcgtgc cgcccgatac cgagccctgc cccttcccgt tggacgtcct cctggcgagg
     4321 atcgcaagcg ccgggttcct gcccgtatct atgcgtttga ccgagacctc ccaggcgtcg
     4381 gtcttgttta acgtgccgga gacg
//
LOCUS       DAOSCR010000167         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12533_length_4396_cov_3.151578, whole genome shotgun sequence.
ACCESSION   DAOSCR010000167 DAOSCR010000000
VERSION     DAOSCR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12533_length_4396_cov_3.151578"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="ftsW"
                     /locus_tag="PLT69_05385"
     CDS             <1..968
                     /gene="ftsW"
                     /locus_tag="PLT69_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOJ13907.1"
                     /translation="RHLAHLVVGAVAMIAAMAVDYRRLARGKLVYALVACAVILLVCC
                     FIPGIGRAGGHAKRWVDLGFFVVQASEFAKLSLVLYFAYHLSRKSRLMHDFSRGALPL
                     LLVVAVVCLLIFAEPDFGTAATVGLWSLGMLVMGGMPLKHLLVLCGTVLPLGLVGLLW
                     EPYRRVRIITFLDPWKDMLGSGYQIIQSMVAFANGGVLGAGLGEGSQKLFYLPEPHTD
                     FILAVVGEETGFLGVLLVTCLFGLWIWRGFSIAQATNDAFGYYAALASVSLVGLQAII
                     NMGVELCLLPTKGLPLPFFSYGGSTLISTMSLCGLVLSVSRRARL"
     gene            965..2017
                     /gene="murG"
                     /locus_tag="PLT69_05390"
     CDS             965..2017
                     /gene="murG"
                     /locus_tag="PLT69_05390"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOJ13908.1"
                     /translation="MRIVVTAGGTGGHVMPAVAIAQAVRDVRPDAEILFVGTTGGMER
                     RIAHSYGLAFRGIPALGVKGKSIANLVRALFVNTASLARALRILADFRPGWVVGTGGY
                     VTGMVMLAGKALGCRLAVQEQNSVPGLTNRILAPLVDRVYLSFPDARRRFPPAKCRIV
                     GNPLRKELLGVRRDPGADRLLVLGGSLGATSINRAAAGAVKILRSRGILLPVTHQCGE
                     RDYGWLKGEYEGMDAEVVPFIDDMAGAYSRAMLAVCRCGGISLAELSHAGVPAIMSPY
                     PYAADDHQLFNGAYVADKGGGWLILDRDLTAERLAGEIAARLADPEGLESASTRMREL
                     GLGTDAQAVAREITDV"
     gene            2010..3380
                     /gene="murC"
                     /locus_tag="PLT69_05395"
     CDS             2010..3380
                     /gene="murC"
                     /locus_tag="PLT69_05395"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ13909.1"
                     /translation="MFRGITRIHFVGIGGIGMSGIAELLLNLGFTVTGSDVAESDSVQ
                     RLRSMGATVHIGHRPENVGDAQVVVYSSAVGLDNPECVSARSRTLPVIPRAEMLAELM
                     RMKHSIAIAGTHGKTTTTSMLATILAHAGKDPTAVIGGKLDMFGSNARLGEGELLVAE
                     ADESDRSFLKLYPVVAVVTNIEEEHMDCYHDIEDIRSTYVQFLNKVPFFGFDMVCLDD
                     PNVQHILPQLERKVFTYGSHTQAIYRFSEPSFEGLNSRFLVHRKDATLGEVSLGVPGV
                     HNCLNALAAIGVSLELGIDFETVREALAGFSGVQRRCHVRGEASGIMVMDDYGHHPTE
                     IRVTLSALRSAFSDRRIVAVFQPHRYTRTRDLFDQFVNAFYDAGMLYITEIYPASEKP
                     IEGVSGKRLFEEIKSHGFRDAFYVPDKDDVPETLSGVLRPGDVVVFLGAGDVWRQGLR
                     LLERLA"
     gene            3377..4216
                     /gene="murB"
                     /locus_tag="PLT69_05400"
     CDS             3377..4216
                     /gene="murB"
                     /locus_tag="PLT69_05400"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOJ13910.1"
                     /translation="MKTMESVPASTLTSMRCGGVIARLFEPESGEELQGLIRAPAGFH
                     LLGAGTNTIFADTTIHTPVIRLGPSFDFIETVDGGLRAGGATHVKKILSRCVKDGLTG
                     MEFMAGIPGTLGGALAMNAGTAKKGIMEVVREIEAVDASGPRTIDPSSLPWGYRRGGI
                     EPGTVVTAAVLALEPSTTDEVRARILEHLEKRRNQPRGPSAGSVFKNPPEAPAGLLID
                     RAGLKGTRVGGAKVSELHANFIVNDEGASATDVAALIALVKRQVFDRFGIELHEEVRF
                     IGQ"
     gene            4206..>4396
                     /locus_tag="PLT69_05405"
     CDS             4206..>4396
                     /locus_tag="PLT69_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13911.1"
                     /translation="MVSDFSDLSHRLRVTSRKRTVQQVNLCLRLGIWAGVLVSVAIIV
                     VCAAAYLANSALFSVRHIT"
BASE COUNT          717 a         1441 c         1493 g          745 t
ORIGIN      
        1 ccaggcacct cgcccacctg gtcgtcggcg ccgtcgccat gatcgcggcc atggccgtgg
       61 actaccgcag gctcgctcgc ggcaagctcg tctacgcgct ggtcgcctgc gccgtgatcc
      121 tcctggtgtg ctgcttcatc cccgggatcg gccgcgcggg cggacacgcg aagcggtggg
      181 tcgacctcgg cttcttcgtg gtgcaggcct ctgagttcgc gaagctctcg ctcgtgttgt
      241 acttcgccta ccacctttcg cgcaagagcc gcctcatgca cgacttctcc cgcggggccc
      301 tcccgttgct cctcgtcgtg gccgtcgtct gcctgctcat cttcgcggag cccgatttcg
      361 ggacggccgc caccgtaggc ctctggtctc tggggatgct cgtcatgggc gggatgcccc
      421 tcaagcacct gctcgtcctc tgcgggacgg tcctgccgtt gggcctcgtc gggctgctct
      481 gggagcccta ccggagggtc aggatcataa cattcctgga cccgtggaag gacatgctgg
      541 gctccggcta ccagatcatc cagtccatgg tggcgttcgc gaacgggggc gtcctcggtg
      601 cgggtctcgg cgaagggagc cagaaactct tctacctgcc ggagccgcac acggatttca
      661 tcctcgcggt cgtgggcgag gagacaggct tcctcggcgt gctgctcgtc acctgcctct
      721 tcgggctctg gatctggagg ggtttctcca tcgcccaggc caccaacgac gcgttcgggt
      781 actacgcggc gctggcctcg gtcagcctcg taggcctcca ggcgatcatc aacatgggcg
      841 tggagctctg cctcctcccg accaagggcc tgccgctccc cttcttcagc tacggcggct
      901 ccaccctcat ctcgacgatg tcgctctgcg ggcttgtcct gtccgtgtcg aggagggcca
      961 ggctatgagg atcgtcgtca cagcgggggg aacgggcggc cacgtcatgc cggccgtcgc
     1021 catcgctcag gcggtccggg acgtgaggcc ggacgccgag atcctcttcg tggggaccac
     1081 cggcggcatg gagaggcgca tagcccactc ctacgggctg gcgttcaggg ggatcccggc
     1141 cttgggcgtc aagggaaagt cgattgcgaa cctcgtgcgg gccctcttcg tgaacacggc
     1201 gtctcttgcg agggcgctgc gcatcctggc ggacttcagg ccgggatggg tcgtgggcac
     1261 cggcgggtac gtcaccggca tggtcatgct cgccggcaag gccctggggt gcaggctcgc
     1321 cgtccaggaa cagaacagcg tgccgggact cacgaacagg atccttgcgc ccctggtcga
     1381 tcgggtgtat ctgtccttcc cggacgccag gcgacgcttc ccccccgcaa agtgcaggat
     1441 cgtgggaaac ccgctcagga aggagctcct gggcgtcagg cgggatcccg gggcggacag
     1501 gttgctcgtt ctcggcggca gcctcggcgc cacgagcatc aacagggccg ccgccggggc
     1561 ggtgaagata ctgaggtcac ggggtatcct cctcccggtg acccaccagt gcggagaacg
     1621 tgattacggg tggctcaagg gtgaatacga gggcatggac gccgaggtcg tccccttcat
     1681 agacgacatg gccggggcct acagcagggc gatgctcgcc gtgtgccgct gcggcgggat
     1741 cagcctggcc gagctctcgc acgcgggggt gccggccatc atgtcgccct acccgtacgc
     1801 cgctgacgat caccagctct tcaacggcgc ctacgttgcg gacaaggggg gaggctggct
     1861 catcctcgac cgggacctca ccgccgaacg tctcgccggg gagatcgcag cgaggctggc
     1921 cgaccccgag gggctcgagt ccgcctcgac acgcatgagg gaactggggc tcggcaccga
     1981 cgcgcaggca gtggcaaggg agatcactga tgtttagggg catcaccagg atacacttcg
     2041 ttggcatcgg cggcattggc atgagcggga tcgccgagct tttgctcaac ctgggcttca
     2101 cggtgaccgg ctcggacgtg gccgagtcag attccgtcca gcgcctgagg tcgatgggcg
     2161 cgacggtcca catcggccac aggcccgaga acgtcgggga cgcccaggtc gtggtctact
     2221 cgtcggcggt cggcctcgac aaccccgagt gcgtgtccgc gcggtcacgg acgctccccg
     2281 tgatccccag ggcggagatg ctcgccgagc tcatgcggat gaagcactcc atagccattg
     2341 cgggcaccca cggcaagacg acgaccacgt ccatgctcgc caccatcctc gcgcatgccg
     2401 gcaaggaccc caccgcggtc atcggcggca agctcgacat gttcgggtcc aacgccaggc
     2461 tcggggaggg tgaactgctg gtcgccgagg cggacgagag cgaccgctcc ttcctgaaac
     2521 tgtaccccgt cgtcgccgtg gtcacgaaca tcgaggaaga gcacatggac tgctaccacg
     2581 acatcgagga catccggtcg acctacgtgc agttcctcaa caaggtgccg ttcttcgggt
     2641 tcgacatggt gtgcctcgac gaccccaacg tgcagcacat cctgccgcag ctggagcgca
     2701 aggtcttcac gtacgggtcc cacacgcagg cgatctacag gttctccgaa ccctcgttcg
     2761 aggggctgaa ctcccgcttc ctggtgcaca ggaaagacgc cacgctcggc gaggtcagcc
     2821 tgggggtgcc gggcgttcac aactgcctga acgcgcttgc ggcgatcggg gtctccctgg
     2881 aactcggcat cgacttcgag accgtgaggg aggccttggc cgggttcagc ggggtccagc
     2941 ggagatgcca cgtgcggggt gaggcctccg ggatcatggt gatggacgac tacggacacc
     3001 acccgacgga gatcagggtg accctctcgg ccctgaggtc cgccttctcg gaccggcgca
     3061 tcgtcgcggt gttccagccg caccgctaca cgaggacgag ggacctcttc gaccagttcg
     3121 tcaacgcgtt ctacgacgcg ggcatgctct acatcacgga gatctacccg gcatcggaga
     3181 aaccgatcga gggtgtcagc ggtaagaggc tcttcgagga gatcaagagc cacggtttcc
     3241 gcgacgcctt ctacgtgccg gacaaggacg atgtgccgga gacgctctcg ggcgtgctca
     3301 ggccgggaga cgtcgtcgtg ttcctgggag cgggcgacgt gtggcggcag gggctcaggc
     3361 tcctggaaag gctcgcatga agaccatgga atccgtgccg gcatcgacgc tcacctccat
     3421 gcgctgcggc ggcgtaatcg cgaggctctt cgaacccgaa agcggtgaag agctgcaggg
     3481 cctgatccgc gcgccggccg ggttccacct gctcggcgca ggcacgaaca ccatcttcgc
     3541 cgacaccacc atccatacgc ccgtgatcag gctcggcccg tccttcgact tcatcgagac
     3601 ggtcgacggg ggtctccgcg cgggaggagc gacccacgtg aagaagatcc tgtcacggtg
     3661 cgtcaaggac ggcctgacgg gaatggagtt catggcgggc atccccggca ccctgggagg
     3721 cgccctggcc atgaacgcgg gcaccgcgaa gaaggggatc atggaggtgg tccgcgagat
     3781 cgaggcggtg gacgcctcgg gccccaggac gatcgatccg tcctccctgc cctggggcta
     3841 ccgcaggggc gggatcgagc ccggcacggt ggtgacggcc gcggttctcg ccctcgagcc
     3901 gtcgacgacg gacgaggtga gggcgaggat cctggagcac ctggagaaga ggcgcaacca
     3961 gcctcgaggc ccgagcgcgg gatccgtctt caagaacccg cccgaggccc cggcgggcct
     4021 cctcatcgac cgggcgggtc tcaagggaac gagggtcggc ggggccaagg tctcggagct
     4081 ccacgcgaac ttcatcgtca acgacgaggg tgcgagcgcg acggacgtgg ccgcgctcat
     4141 agccctcgtg aaacgacagg tgttcgacag gttcggcata gagctccacg aggaggtcag
     4201 gttcattggt cagtgacttc agcgatctct cccacaggct gagggtcacc tcgaggaaga
     4261 ggaccgtgca gcaggtgaac ctgtgcctgc ggctcggcat ctgggcgggc gtcctcgtgt
     4321 ctgtcgccat catcgtcgtg tgcgcggccg cctacctcgc caactctgcc ctgttctcgg
     4381 tgaggcacat aacgat
//
LOCUS       DAOSCR010000168         4395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12537_length_4395_cov_2.999309, whole genome shotgun sequence.
ACCESSION   DAOSCR010000168 DAOSCR010000000
VERSION     DAOSCR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12537_length_4395_cov_2.999309"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLT69_05410"
     CDS             complement(<1..1023)
                     /locus_tag="PLT69_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ13912.1"
                     /translation="MRYTEARKRHWDAIAVRKDSWRSRGEPYHRRLEAVYRHIVSPNL
                     RVLEIGCGTGELLAALEPSFGMGVDFSSEMIERAKRRHPHLHFLCADAHALPIEETFD
                     VIVLSDLVNDLWDVQAVLENLARLSHPRTRVVINTYSLLWEIPLAAVRMLGLATPVLR
                     QNWLTVHDLTNMLNLAGFEVIRRFQEVLWPFEEHGPLARFLNRVLVKVKPFSHLALTN
                     VIVARPKGRPMEAEPVVSVVVPARNEAGNIEAIFERVPEMGAGTQIVFVEGNSTDDTY
                     AAIERAIEAHPERRAVLFRQKGKGKGDAVRLGFEKASGDVLMILDADLTVPPEDLPRF
                     YEAVRSG"
     gene            complement(1067..2020)
                     /locus_tag="PLT69_05415"
     CDS             complement(1067..2020)
                     /locus_tag="PLT69_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOJ13913.1"
                     /translation="MEKKARIYVAGHTGLVGSAVVRRLKAEGYTNVLTAPHASLDLTR
                     QADVDAFFDDRRPEYVFLCAARVGGILANSTYPAWFIYDNIMIAANVIHASFRTGVKK
                     LLNLGSSCIYPKFAPQPLREDALLTGPLEPTNEAYAVAKIAAIKLCRYYNAQYKTDYL
                     SVMPTNLYGPFDNFDLESSHVLPALIRRFHEARIRGDGSVVLWGTGTPRREFLHVDDL
                     ARACVFLMQNYSAEDIGEFVNIGTGTDVTIAELAGIVAGVVGYWGRIEWDTSKPDGTP
                     RKLLDVSRIRSLGWEPAIGLSQGIADTYRWFLDNFERIRGT"
     gene            complement(2066..3184)
                     /gene="gmd"
                     /locus_tag="PLT69_05420"
     CDS             complement(2066..3184)
                     /gene="gmd"
                     /locus_tag="PLT69_05420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ13914.1"
                     /translation="MKKALITGITGQDGAYLAEFLLGKGYEVHGIKRRSSSFNTYRVD
                     HLYQDPHEQNVKFFLHYGDLTDATNIIRIIQDVCPDEIYNLGAQSHVQVSFETPEYTA
                     QSDALGTLRLLEALRILGMADRVRFYQASTSELFGKVQKIPQDEETPFYPRSPYGVAK
                     LYAYWITVNYREAYGMFACNGILFNHESPIRGETFVTRKVTRAAARVALGLQKKLYVG
                     NLGALRDWGHARDFVRAQWLMLQQERPKDYVIATGVQHSVRELCTLAFAEAGIGLEWK
                     GSGADEKGVVSGTRPPCEAGGPAPGEVVVEVDPRYFRPTEVDTLLGDATRARRELGWE
                     PTVTFEEMIGEMVRHDMEEARMDVLCRDSGYRILNRQE"
     gene            3764..>4395
                     /locus_tag="PLT69_05425"
     CDS             3764..>4395
                     /locus_tag="PLT69_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOJ13915.1"
                     /translation="MGVLRVILALSVLLSHVGGILISSGGRIALYFGTGGEVAVQCFY
                     ILSGFYMALILDGKYGRTAEGTLLFYTNRLFRLMPLYLLFLCVSFCLSYLATVSYSFP
                     KQPATDLILHHDRLGIGALAYVALSNLFVFGHDLVIFFLGIDPADGSLFFTTRLPETP
                     RLAIELFFIPQSWTLSLEFSFYLLAPFLVGRTRLSAVLVVASLCLRALLYS"
BASE COUNT          670 a         1451 c         1416 g          858 t
ORIGIN      
        1 gcccgagcgg acggcttcat agaagcgcgg caggtcttcc ggcggcacgg tgaggtcggc
       61 gtccaagatc atgaggacgt cgcctgaggc cttctcgaac ccgagcctca ccgcgtcccc
      121 cttccccttg cccttctgcc tgaagaggac cgccctcctc tcggggtgcg cctcgatggc
      181 cctctctatg gcggcgtagg tgtcgtcggt ggagttcccc tccacgaaca cgatctgtgt
      241 gcccgcgccc atctcgggca cgcgctcgaa gatcgcctcg atgttgccgg cctcgttgcg
      301 cgcgggcacc acgacgctga ccacgggctc cgcctccatg ggcctgccct tgggcctcgc
      361 cacgatcacg ttggtcagcg ccaggtggct gaagggcttg accttcacga ggacccggtt
      421 cagaaaccgc gcaaggggcc cgtgttcctc gaacggccag agcacctcct ggaagcgcct
      481 gatcacctcg aatcccgcga ggttcagcat gttcgtcagg tcgtgcacgg tgagccagtt
      541 ctgcctgagc acaggggtgg cgagccccag catgcgcacg gccgcgagcg ggatctccca
      601 gaggaggctg taggtgttga tcacgacgcg cgtccttggg tgggagagcc tcgcgaggtt
      661 ctcgaggacc gcctgcacgt cccacaggtc gttcaccagg tcggacagga cgatgacgtc
      721 gaaggtctcc tcgatgggaa gcgcgtgggc gtccgcgcac aggaagtgca ggtgggggtg
      781 gcgtctcttg gcccgctcga tcatctctga ggagaagtcg acccccatgc cgaaagaggg
      841 ctcgagcgcg gcgagcaact cgcccgtgcc gcacccgatc tccagcacgc gcaggttggg
      901 actcacgatg tgcctgtaga ccgcctcgag cctcctgtgg tagggctcgc cacgggatcg
      961 ccaggagtcc ttcctcacgg cgatggcgtc ccagtggcgc ttccttgcct cggtgtaccg
     1021 caagaaggca tcctgagcgg gcgattgagc ttttcccgtc gacatgtcat gtcccccgta
     1081 tgcgttcgaa gttgtccagg aaccagcggt acgtgtccgc gatcccttgg ctaagcccga
     1141 tggccggctc ccagccgagc gagcggatgc gggacacgtc gaggagcttc ctcggtgtcc
     1201 cgtcgggctt cgaggtgtcc cactcgatgc ggccccagta cccgacgacc ccggccacga
     1261 tgcctgcgag ctccgctatg gtgacgtccg tgccggtccc tatgttcacg aactcgccga
     1321 tgtcttctgc ggaatagttt tgcatgagga agacgcaggc cctcgccagg tcgtccacgt
     1381 ggaggaactc gcgcctgggc gtgcccgtgc cccagaggac caccgagccg tcccccctga
     1441 tcctcgcctc gtggaaccgc ctgatgagcg cgggcagcac gtgggaggac tcgaggtcga
     1501 agttgtcgaa aggcccgtag aggttcgtgg gcatgaccga gaggtagtcc gtcttgtact
     1561 gggcgttgta gtacctgcag agcttgatgg ccgcgatctt ggccacggcg taggcctcgt
     1621 tcgtgggctc gagcggcccc gtgagcaggg cgtcctccct caagggctgg ggagcgaact
     1681 tggggtagat gcaggacgac ccgaggttga ggagcttctt cacccctgtc ctgaaggagg
     1741 cgtggatcac gttggccgct atcatgatgt tgtcgtagat gaaccaggcg gggtacgtgc
     1801 tgttggccag gatgccgccg accctggcgg cgcacaggaa gacgtactcc gggcgccggt
     1861 cgtcgaagaa cgcatcgacg tcggcctgcc gggtgaggtc gaggctcgcg tgcggcgcgg
     1921 tcagcacgtt cgtgtacccc tcggccttaa gcctcctgac aacggccgac cccacgaggc
     1981 ccgtgtgccc ggccacatag atcctcgctt tcttctccac gtccgtcccc cgtgtccccg
     2041 gcaggcgcgc tcccccgcct ccagcctact cctgcctgtt gaggatccgg tagccgctgt
     2101 cgcggcacag cacgtccatc ctcgcctctt ccatgtcgtg cctcaccatc tccccgatca
     2161 tctcctcgaa ggtgaccgtc ggctcccagc ccagctcgcg ccgcgccctc gtcgcatcgc
     2221 ccaggagcgt gtccacctcg gtgggacgga agtacctggg gtccacctcg acgacgacct
     2281 cgccaggcgc cgggcctccc gcctcgcagg gcggcctggt cccggacacg acccccttct
     2341 cgtcggcccc tgagcctttc cactcgaggc cgatccccgc ctcggcgaac gcgagggtgc
     2401 agagttcgcg caccgagtgc tggacgccgg tcgcgatcac gtaatccttc ggcctttcct
     2461 gctggagcat gagccattgg gccctgacga agtccctcgc gtgtccccag tccctgagcg
     2521 cgcccaggtt gcccacgtag agcttcttct ggaggccgag ggcgactcgg gcggcggctc
     2581 gggtgacctt cctggtcacg aacgtctctc ccctgatggg agactcgtgg ttgaagagga
     2641 tcccgttgca ggcgaacatg ccgtacgcct cccggtagtt caccgtgatc cagtaggcgt
     2701 agagcttcgc caccccgtag gggctcctgg ggtagaaggg ggtctcctcg tcctggggta
     2761 tcttctgcac ctttccgaag agctcgctcg tggatgcctg gtagaagcgc acccggtccg
     2821 ccatgcccag gatcctgagg gcctcgagga gcctcagggt gccgagagcg tccgactggg
     2881 ccgtgtactc gggcgtctcg aacgagacct ggacgtggct ctgcgcgccc aggttgtaga
     2941 tctcgtcggg acagacatcc tggatgatgc ggatgatgtt cgtcgcgtcc gtcaggtcgc
     3001 cgtagtggag gaagaatttc acgttctgct cgtgggggtc ctggtagagg tggtccaccc
     3061 ggtaggtgtt gaagctggac gagcggcgct tgatgccgtg cacctcgtac cccttgccga
     3121 gcaggaactc cgccaggtag gcaccgtcct gccccgtgat cccggtgatg agcgccttct
     3181 tcatcggacg tcctccctaa ccacatgttc ggaaaacatg gaaaggcagt tgaaacacat
     3241 accacgacag gccggctcgt gtcacgtccc gccgggcgtc tggaggctcg ccgcacggta
     3301 tgccggcgcc atcccctccg cgcgggggaa gatgggaggc gatgcagaac ggggggctgg
     3361 atccacccct cacccgcccc acgggccgca gacggtgaac ggggtcgtcc aggcggggcg
     3421 taagccctgg ccggggacac gaaacactgc gcatgggacg ctttgccggg cgccgcctgt
     3481 catggccggg cgatgtctcg cgcacgtgcc ggcactcacc cggctatcga tggaaagggg
     3541 ccggggcccg gaccgggcgc accgcatcat tgcaagggat agagcccgca gtttgccctt
     3601 gtgcgggctc ggggtagtac gctaaggttc tccaggctat gcgcatgggc cgagtctgcg
     3661 caccggcagg ttccatgcct gaaggccgtg tacaaagacc tctgcattat tggtttcagt
     3721 aagccgggga tatggttgtc aaacgtacgt ggggttgagt tgaatgggcg tgttacgcgt
     3781 gatcctcgca ttgtcagtcc tgctctcgca cgtggggggg atcctgatct cttcgggcgg
     3841 caggattgcc ctgtatttcg gtactggtgg ggaggtggcg gtccagtgct tctacatcct
     3901 ctccgggttc tacatggcgc tgatcctcga cggcaagtac ggacgcaccg ccgaggggac
     3961 gctcctcttc tacacgaaca ggctcttcag gctcatgccc ctgtacctgc ttttcctctg
     4021 cgtgtccttc tgcctgtcct acctggccac ggtgagctac agtttcccca agcagcctgc
     4081 gaccgacctg atcctccacc acgacaggct gggcatcggc gccctggcct atgtcgcgct
     4141 gtcgaatctc ttcgttttcg gccatgacct ggtgatcttc tttttgggca tcgaccctgc
     4201 cgacgggtcg ttgttcttca ccacgaggct gccggaaacc cccaggctgg ccatcgagct
     4261 cttcttcata ccgcagtcat ggaccctgag cctggagttc tccttctacc tgctggcccc
     4321 gtttcttgtc gggaggacgc gcctttccgc ggtgctggtg gttgcgagcc tgtgcctgcg
     4381 cgccctgctc tactc
//
LOCUS       DAOSCR010000169         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12594_length_4378_cov_4.304881, whole genome shotgun sequence.
ACCESSION   DAOSCR010000169 DAOSCR010000000
VERSION     DAOSCR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12594_length_4378_cov_4.304881"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLT69_05430"
     CDS             <1..674
                     /locus_tag="PLT69_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ13916.1"
                     /translation="PFLRKVYEGWYSWIARNVPQGPGAVVELGSGAGFLDEYIPGLIT
                     SEVFLCPHVRVVLDGRALPFRDSSLKAVAMVDVFHHVPDVRALLQEASRCLMPGGRIL
                     MVEPWMTPFSRRVYTRLHHEPVDMEAGSWSFPSSGPLSGANTALPWIVFERDLETFRR
                     DFPGLELEGIDIVNPFSYLLSGGVSTRSLVPGFFFPAVDLVERILSRRAKTLGMFARI
                     SVRRR"
     gene            678..2381
                     /locus_tag="PLT69_05435"
     CDS             678..2381
                     /locus_tag="PLT69_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ13917.1"
                     /translation="MGPRQAPEKQGSRASRLEAFFPLALAVCTAIAYAPTFKNGFVHL
                     DDPAQLTQNARVLSGVSWDTFLWSFTGESFAQPLTVLAYALLNSLFGLNPAVFHGASL
                     LLHVANALLLFSFFKKATGDFLPSAAVASLFALHPVNVESVAWVAELNNVLSGTFFML
                     TLHAYLWYAQRPTPLAYLATASALILGLFSKQVLMTVPFLLLLLDLWPLRRVSIQRVG
                     KKVRLEGVPATRLVAEKIPLVAVSMAVLATAIAGLESHNSFIGTSLVPVGLRVSNAVL
                     SYFRYLEKLFIPAGLAPLYAYPRSIALWKAAAALAAILAASAYALREAGRRPYLAVGW
                     FWFLGVMAPFLGLIQAGQWPEMAERYAYLTTIGVFVVLAWGAKEAAASRRSLGRVLVA
                     LALAAVTACAVLTWRQVGFFRDSRVLFERVVQVHPQNPMGHRNLGTELLQAGDIEAAI
                     ASYERAIALDPTFPVNHYNMGLALTMAGRLDEAIASYEKGIALDEAGGVPEGSPDLDY
                     VQKNRVDFLSQAHNNLGYLLGARGRTEDAVRHLTRALELNPANARARSNLEKITGAAL
                     Q"
     gene            complement(2898..3920)
                     /locus_tag="PLT69_05440"
     CDS             complement(2898..3920)
                     /locus_tag="PLT69_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOJ13918.1"
                     /translation="MRKGQASLVCLMVVVLCAVSTDLFALDRPYRIEVLQVTNIGPFQ
                     ASLTGFVQELQQNGLVQGKNIDIRRTVIDFDVEKAGLWSKVGVLLRITGETQKIVGRK
                     PDLVLTIGTPATKYAKDMIVKAGIPLVFAGVAIPTAAGCKSQKEAGPGFTGATLYMDM
                     DNVIKIIKLAFPNIKKIGVIHSEDENAVAQVSLLKKSAGSAGIQVLSRLIDKNARITP
                     VAEELNSQGIEATIIPLDTYYGMRNYEATNDLRNLNMKWKKPGICLVYWKSPGAVLYI
                     GADFKHVGALAGRQAAKILLEGVKPETLPIAHQEDLGIMVDINEMKKLNIQLPLQILQ
                     IAKSVE"
BASE COUNT          737 a         1359 c         1357 g          925 t
ORIGIN      
        1 agccctttct caggaaggta tacgagggct ggtattcgtg gatcgcccgc aatgtccccc
       61 aggggccggg cgccgtggtc gagctcggct cgggcgcggg gtttctcgac gagtacatac
      121 ccggccttat cacctcggag gtcttcctgt gcccgcacgt cagggtcgtg ctcgacggca
      181 gggcgctccc cttcagggac tcctcgctga aggccgtcgc catggtggac gtgttccatc
      241 acgtgccgga tgtgcgcgca ctcctacagg aggcatccag gtgcctcatg ccgggggggc
      301 gcatcctcat ggtggagccg tggatgacgc ccttttcgag acgcgtctac acgaggctcc
      361 accacgagcc ggtggacatg gaggccgggt cctggtcctt cccgtcctcg ggcccgctct
      421 ccggggccaa caccgcgctg ccgtggatcg tgtttgaaag agaccttgag accttcagga
      481 gggacttccc cggcctcgag ctcgagggga tcgacatcgt caatccgttc tcctacctcc
      541 tctcaggtgg cgtctcgacg agatccctcg tgcccgggtt tttctttccc gccgtggatc
      601 tcgtggaacg catcctctcg cgacgagcca aaaccctcgg catgttcgcg aggatttccg
      661 tcaggaggag gtgaggcgtg ggcccaaggc aggccccaga gaagcaagga agccgggcct
      721 cgaggcttga ggccttcttc cctctcgcgc tcgccgtctg caccgccatc gcctatgccc
      781 ccaccttcaa aaacggcttc gtccacctcg acgacccggc ccagctcacg cagaacgcga
      841 gggtcctctc cggggtctcg tgggacacct tcttgtggtc cttcaccggg gagtcgttcg
      901 cgcagccgct cacggtactc gcctacgcgc ttctgaactc cctgttcggc ctgaaccccg
      961 cggtcttcca cggcgcgagc ctgctcctcc acgtcgccaa cgcgctcttg ctcttctctt
     1021 tcttcaagaa ggcgacggga gacttcctgc ctagtgcggc cgtcgcctcc ctgttcgcgc
     1081 tccacccggt gaacgtggag tcggtcgcgt gggtggcgga gctgaacaat gtcctgagcg
     1141 gcaccttctt catgctcacc cttcacgcgt acctgtggta tgcgcagagg ccaacgccgc
     1201 ttgcgtacct ggcaacggcc tcggcgctca tcctcgggct tttctccaag caggtgctca
     1261 tgaccgtgcc ctttctcctg ctgcttctcg atctctggcc cctcaggcgg gtgagtatac
     1321 agagggtcgg caagaaagtg cgcctcgagg gcgtgccggc aaccaggctc gtggcggaga
     1381 agatccccct cgtcgccgta tccatggccg ttctcgccac agccatcgcc gggctcgaga
     1441 gtcacaacag cttcatcggc acgtcgctcg tgccggtggg cctccgtgtc tcgaacgcgg
     1501 tcctgtccta tttcaggtac ctcgagaagc tcttcatccc ggcaggcctc gcccccctgt
     1561 acgcctaccc ccggtccata gccttgtgga aggccgcggc cgcgctggcc gccatcctcg
     1621 cagcgagcgc atacgcgctc agggaggcgg gaaggaggcc ctacctggcc gtgggctggt
     1681 tctggtttct cggggtcatg gcgccgtttc tcggcctcat ccaggccggc cagtggcctg
     1741 agatggcgga gcggtacgcc tacctcacca cgatcggcgt cttcgtcgtg ctcgcctggg
     1801 gtgcaaagga ggctgccgcc tcgagaaggt ctctcggccg cgtcctcgtt gccctggcgt
     1861 tggcggccgt cacggcgtgc gccgttctca cgtggcgcca ggtggggttc ttcagggaca
     1921 gcagggtgct cttcgagcgc gtcgtacagg tccacccgca gaaccccatg gggcaccgaa
     1981 acctcggcac cgagctcctc caggcgggcg acatcgaggc cgcaatcgcg agctacgagc
     2041 gtgcgatagc gctggacccg acgtttccgg tcaaccatta caacatgggt ctcgcgctga
     2101 ccatggccgg ccggctggat gaggcgatcg cctcctacga gaagggcatc gccctggacg
     2161 aggcgggagg cgttcccgag ggatccccag acctcgacta tgtgcagaag aaccgcgtcg
     2221 acttcctctc gcaggcgcac aacaacctcg gctacctcct gggtgccagg ggcaggacag
     2281 aggatgcggt ccgtcacctc acccgtgccc tcgagctgaa cccggcgaac gcgagggccc
     2341 ggagcaatct cgaaaagatc acgggtgcgg ccttgcagtg aggacatggg tctggatgcg
     2401 tatatgatac gctgaatgaa gggtttggcg gaagacaagc cggaagctct cggcgccacc
     2461 atcctgcgtt tgggtggata tcggttcgtc atcacccctc gagttgtcag atccgggatc
     2521 agggaagggg ccgctgtttt tccagcatgt ctcgtaccct cgatgcaggt ccctgcccct
     2581 cgggggaatg caccctcatt cttgtcgcta tccctctgtc ctggctccag tgggcagtcc
     2641 gcgtgtcgtc tttctggttg gccacctgca taagaacaga gggcggggtt ttctgcgggg
     2701 tggtcgatcc ccgcgatcgt gaaggcgatg atggcggcgt ccaatcggta cggcaaggca
     2761 tacggggcga gggacgtgac ggtcgacgca acggcgggca tgcgggtctc ccctggaacg
     2821 cccccgcccc tggtggcatc tcgtgcagag aggggcggga agatcagtgg aacagtcggc
     2881 cgcccgggcg accttgccta ttcgaccgac ttcgcaattt gcaggatctg caaggggagc
     2941 tggatattga gctttttcat ctcgttgatg tccaccatga tcccgagatc ctcctggtgc
     3001 gctatgggga gggtttcggg cttcacgccc tcgaggagta ttttcgcggc ctgcctgccg
     3061 gcgagggcgc cgacatgctt gaagtcggcg ccgatgtaga ggaccgcgcc cggcgatttc
     3121 cagtacacga ggcagatgcc cggcttcttc cacttcatgt tcaggttcct caggtcgttg
     3181 gtggcctcgt agtttctcat gccgtagtag gtgtcgaggg ggatgatggt ggcctcgatg
     3241 ccctggctat tcaattcctc cgcaaccggc gtgatgcgcg cgttcttgtc gatgagcctc
     3301 gagagcacct gaatgcctgc cgacccggcg ctcttcttca gcagcgagac ctgtgccacg
     3361 gcgttttcgt cctcgctgtg aatgaccccg atcttcttga tgttcgggaa ggcgagtttg
     3421 atgatcttga tcacgttgtc catgtccatg tacagcgttg cgccggtgaa ccccggccct
     3481 gcctccttct gggatttgca gccggcggcg gtgggtatgg cgacgcctgc gaacacgagg
     3541 ggaatgccag ccttgacgat catgtccttg gcgtacttcg tggcaggggt cccgatggtg
     3601 agcacgaggt cgggcttgcg acctacgatc ttctgggttt ccccggtgat cctcaggaga
     3661 acgccgacct tgctccacag gccggccttc tccacgtcga agtcgatgac ggtcctgcgg
     3721 atgtcgatgt tcttgccctg cacgaggccg ttttgctgga gctcctgcac gaatcccgtg
     3781 agcgaggcct ggaacggccc gatgttcgtc acctggagga cctcgatgcg gtagggcctg
     3841 tcgagggcga acaagtcggt ggagacagca cagagaacga cgaccatcag acacacgagg
     3901 gatgcttgtc cttttctcat gtcaacctcc cctgagggtg tcattcctgc cgctcgagac
     3961 gacgcgacac aaggtacccg atgaaactct agtggaggag aaattatatg tcaatgtcca
     4021 tatgatcata tcagacatag agaggcacgg gtatatgaat tcaggaaaag acggttatcg
     4081 ttgattatca acgggtggtt atccaaggaa agagaacaga tcggcgaact tggggcctca
     4141 accgaagcat tctgaccgag tatccccttt aattgcagtg tgtaaatagt catgagtgga
     4201 ctccttgagg gatccactga tttagcgtaa tagatatgaa ccatctcctt cataagaaac
     4261 ctagtttcat cggaagaggg tgcgttaatg ccggcctcgc ttcgctcgtg cctacggcag
     4321 tattggtggt gtcaagaatg tttcgcactt ttgatccagg tctctgtcct tcggagta
//
LOCUS       DAOSCR010000170         4368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12636_length_4368_cov_3.305588, whole genome shotgun sequence.
ACCESSION   DAOSCR010000170 DAOSCR010000000
VERSION     DAOSCR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12636_length_4368_cov_3.305588"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PLT69_05445"
     CDS             complement(<1..1079)
                     /locus_tag="PLT69_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOJ13919.1"
                     /translation="MNDILGGHLVGKYLREVEKVDTVFCLSGGHIEAILDSFTEYRIR
                     AIDVRHEQACAMMAHAWSIYTGRPGVCLVTAGPGFTNALTGIVNAFLDNAPVVVLCGR
                     HPLRDDLKGALQEMNQIDMVKPVTKWAATCYDTKRIPEYLAAAFRNASSGRPGPVFLE
                     LPPDILNVKVPVEKAPMPKAPARKFTVHPDDWDLKQAADVINAAKKPLLMGGSGVGMS
                     CCSVALGQFVERTGIPFILLNNGRGELADNHPLSLWDVGNTGLMLGLTQADVLVAVGV
                     RFNWLLQSGAVIPPHVKVVRIDIDPTEIDRNRTADVGLVGDAQSVLNQLLPLVKRADH
                     SEWVDMLRKASRSFLETELRLRSSPS"
     gene            complement(1173..1529)
                     /locus_tag="PLT69_05450"
     CDS             complement(1173..1529)
                     /locus_tag="PLT69_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13920.1"
                     /translation="MKLALLLFGLYLKLARAAKTNKAFRNYIGNIQLRILIKTSDNRW
                     GRLFVFDKGRVTSASGANHAFDAALVWIDASTAFKVMLSGSDEATFMAAASGKLRVEG
                     MAYYIQWFNDAVKLVM"
     gene            1786..2346
                     /locus_tag="PLT69_05455"
     CDS             1786..2346
                     /locus_tag="PLT69_05455"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13921.1"
                     /translation="MEAGLRLFGEKGLAGTSSNEIARRAGVSIGTFYSYFKDKRSLFL
                     AILKMHLDTFVTGIYLVEQQGDPSIDSIIRDHVQRAFAIFDRQLDFHRQALVMKFTDP
                     EVRRLFEEVEEKQLSLITALLERHTKPSKNRDLKVVAKVIHGAVENAAHAVKFLASPF
                     KEERFTEELTSMIQSYVEGLTERERG"
     gene            2900..4168
                     /locus_tag="PLT69_05460"
     CDS             2900..4168
                     /locus_tag="PLT69_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ13922.1"
                     /translation="MCLNATIGHPSACMQEIRVPEGVPHAWDELLETARCVLGASSAF
                     IVRTVAPRLEIVSSASPKKAQGSSLSLAARVRRQCEEAAASGEVVVRTSRSHRRHGRG
                     SSPTLSSTISAPLSWPTGAPFGAVCVVGVQGCPCTKEELAGIVRAFSRLATRILENAL
                     LSADLEAALREVSRLAEERERFLGVAAHDMKNALNAFLGCCRHLLPGADELDARRRRF
                     LELAHSSATTLIQLMDELMDIARIESAARAPSSSTPDAREVILECIHMHRHLAEAKGI
                     MIDLVLPKTDITAPIDSTLLACVMNNLLSNAVKFSPNGASVVVSVEVQQGNVVVRVTD
                     HGPGIPREEQPRLFRPFQKASPRPSGEETSTGLGLYIARRIVEGHGGSIWVESEPGKG
                     ATFSFTISSSSSTRPAGAEKEGTADPGVRP"
BASE COUNT          829 a         1436 c         1346 g          757 t
ORIGIN      
        1 gaggggctcg agcgaagcct gagctcggtc tccaagaacg aacggctcgc cttcctgagc
       61 atatccaccc actccgagtg gtccgccctc ttgacgagcg gcaggagctg gttgagcacc
      121 gattgcgcgt ctcccacgag ccccacgtcc gcggtcctgt tgcggtcgat ctcggtgggg
      181 tcgatgtcta tgcgcacgac cttcacgtgg ggggggatca ccgctcccga ctgcaggagc
      241 cagttgaacc tcacgcccac ggccacgagc acgtccgcct gggtgagccc gagcatcagg
      301 cccgtgttcc caacgtccca gagggagagc gggtggttgt ccgcaagctc gcccctgccg
      361 ttgttcaaga ggatgaacgg tatgccggtc cgttccacga actggccaag cgcaactgag
      421 cagcagctca tgcccacccc gctgccgccc atgaggaggg gtttcttcgc cgcattgatg
      481 acatccgcgg cctgcttgag gtcccagtcg tccgggtgga ccgtgaactt cctggccggc
      541 gccttgggca tcggcgcctt ttccaccggg accttcacgt tgaggatgtc cggggggagt
      601 tccaggaaca ccgggcccgg ccttccgctc gaggcgttcc tgaacgcggc agccaggtac
      661 tcgggaatcc tcttcgtgtc gtagcaggtg gccgcccact tggtcacggg cttgaccatg
      721 tctatctggt tcatctcctg gagcgcgccc ttgaggtcat ccctcagggg gtgcctgccg
      781 cacaggacca cgaccggcgc attgtcgagg aaggcgttca cgatccccgt cagggcgttg
      841 gtgaagccgg gccctgcagt gaccaggcac accccgggcc tgcccgtgta tatggaccag
      901 gcgtgggcca tcatggcgca cgcctgctcg tgcctgacgt ctatggcacg gatcctgtac
      961 tcggtgaagc tgtcgaggat cgcctcgatg tgcccgccgg agaggcagaa aaccgtgtcc
     1021 accttttcca cctccctgag atacttcccc accaggtgac cgcccaggat atcgttcatg
     1081 ggtcatcctc ccctgtcata gtacctgcga aatcgttcat cgtaagggcc gcgtccgttc
     1141 gcacggaagt cgcgccgcgg ccgcgtgggt tgctacatca cgagcttcac cgcatcgttg
     1201 aaccactgga tgtagtaggc catcccctcc accctgagct tgccggaggc cgccgccatg
     1261 aaggtggcct cgtcgctgcc cgagagcatc accttgaagg ccgtggacgc gtcgatccag
     1321 acgagggccg cgtcaaaggc gtgattggcg cccgaggccg aggtcaccct ccccttgtcg
     1381 aagacgaaga gcctgcccca cctgttgtcg gaggtcttga tgaggatcct gagctggatg
     1441 ttgccaatgt agttcctgaa cgccttgttc gtcttcgccg ccctggcgag cttgaggtag
     1501 agcccgaaaa gaagtaacgc cagcttcatg gaaccctccc tgcacgcatt tcaagaatgc
     1561 acagacactc gcggcccccc atgcatcaac gcttctgacc gcgaatgacc gaccttgtca
     1621 taccattata ctacctaggc catgcttgtc aagcaaatat gagcaagagc tcatatattg
     1681 atccatggcc tttcttgtgg tatgggtgac ctacggcgtg gtggccaagc gatgcgtgtc
     1741 cgaacacccc aacagaagag gagcagggag agcaaacaac gcatcatgga ggccggtctc
     1801 cgtctcttcg gcgaaaaggg tctggcaggg accagttcca acgagatcgc caggagagcc
     1861 ggggtatcca tcgggacctt ctacagttac ttcaaggaca agcggagcct cttcctcgcc
     1921 atcctcaaga tgcacctaga cacgttcgtc acaggcatct acctcgtgga acaacaaggc
     1981 gacccatcca tcgactcgat catcagggac cacgtacaga gggccttcgc catcttcgac
     2041 aggcagctcg acttccacag gcaggccctc gtgatgaagt tcacggaccc ggaagtcagg
     2101 aggctcttcg aggaagtgga ggagaaacag ctctccctca tcacggcact cctcgagcgc
     2161 cacacgaagc cgtcgaaaaa cagggacctg aaggtggtcg ccaaggtcat ccacggggcg
     2221 gttgagaacg cggcacacgc ggtgaagttc ctcgcatcgc ccttcaagga agagcggttc
     2281 acggaagaac tcacctcgat gatccagagc tacgtcgagg ggctcacgga acgcgagcgg
     2341 ggatgaacgc gcctgtcggc gcccggcgcc tgccacgcgg agatcaccct tctttacgct
     2401 gacgccaagc gtatgcgccg gccggccgat gcgccgaccg gcgccgatga gggaagggat
     2461 ctccctgtga aggcgccctc gcagacttga atccctcccc acaagagggg cctatcctca
     2521 gggagaaaga cgcacacgac gcttcatcgt tcaagcccgc gcgagacaaa cccctgcgca
     2581 ggcgtgcccg ccatgggagg ctcgcctgcg agggcgaccg gccacatagt gggagggacg
     2641 accgggtccc caagggaagg cccccaaggc gttcgtcgtc cctgggcccg ttgacgatcc
     2701 tggcaaatca ggtgtcggag cgactcgttg gaaggcgccc cgggcgcccg cagtccctcc
     2761 ccgaggccgg gcacacgtga cgccagcagg accatgacct agggcaccct ttccttttcc
     2821 atcgagggca ttataatgag gacccaggga agtcattgaa tcttggaatg cacctgcact
     2881 gcagtgaagg cggtcatgca tgtgcctaaa cgcgacgatc ggacatccct cggcatgcat
     2941 gcaggagatc cgcgtccccg agggcgtccc tcatgcctgg gacgagctcc tcgagaccgc
     3001 gagatgcgtc ctgggcgcat cctcggcctt tatcgtacgt accgtcgctc ctcgactcga
     3061 gatcgtctcc agcgcgtccc cgaagaaggc gcaggggagc tccttgtcgc tggcagcccg
     3121 ggtcagaagg cagtgcgaag aggcggcggc ctccggcgag gtcgtggtca ggacatcgag
     3181 gtcgcacagg cgccacgggc gtggatcctc ccccaccctg tcgtccacca tctcggcgcc
     3241 cctttcttgg ccgaccggcg ccccgttcgg ggccgtgtgc gtcgtgggcg ttcagggatg
     3301 tccttgcacg aaagaagagc tcgcgggcat cgtgcgggca ttctcgcgtc tcgcaacccg
     3361 aatcctcgag aacgccctct tgtcggccga tctcgaggcg gcgctcaggg aggtctccag
     3421 gctggccgag gagagggagc gtttcctcgg cgtggccgcg cacgacatga agaacgccct
     3481 gaacgccttc ctcggctgct gcaggcacct tcttcccgga gccgacgagc ttgacgcgcg
     3541 caggagaagg ttcctcgagc tggcgcacag ctcggcaacg accctcatcc agctcatgga
     3601 tgaactcatg gacatcgcga ggatcgagag cgcagcgagg gcgccgtcga gttccacccc
     3661 ggacgccagg gaggtcatcc tcgaatgcat ccacatgcat cgacacctgg ccgaggcaaa
     3721 gggcatcatg atcgacctcg tgctcccgaa gaccgacatc acggcgccca tcgacagcac
     3781 cctgctcgcg tgcgtgatga acaacctcct gtcgaatgcc gtgaagttct caccgaacgg
     3841 cgcctcagtg gtcgtctccg tggaggtcca gcagggaaac gtcgtcgtga gggtgaccga
     3901 tcatgggccc ggcatcccgc gggaggaaca gcctcgcctg ttcaggccct tccagaaggc
     3961 cagcccgaga ccttccggcg aggagacgag tacggggctc ggcttgtaca tcgcccgaag
     4021 gatcgtcgag ggccacggag ggagcatctg ggtcgagagc gagccgggca agggggccac
     4081 gttctccttc accatctcga gctcgtcgtc cacccgtccc gccggtgcgg aaaaggaggg
     4141 gacggccgac ccgggcgtgc ggccctgacg ggcatcctgg tcgaaccccg cacgtctgtg
     4201 ggcgcaaaga cgccggcgct cgcatgaagc ccggaccgag gacatccata ccacaccctc
     4261 cgaaaggtgg catgtgccga aagtctttcg cggtgatggt ggcacgggcc tttctcccca
     4321 tcacccatgg catgccggac gagccccctc accctcgaac atcccgcc
//
LOCUS       DAOSCR010000171         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12676_length_4354_cov_3.218190, whole genome shotgun sequence.
ACCESSION   DAOSCR010000171 DAOSCR010000000
VERSION     DAOSCR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12676_length_4354_cov_3.218190"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLT69_05465"
     CDS             <1..318
                     /locus_tag="PLT69_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ13923.1"
                     /translation="LYLHSAPEGRKIDLIRKNPKVCFGVESTCEVVPSDRACSFTMRY
                     ESVVGKGTARIVTDEAEKRRALEVITGRYAHEATPLPEGATGKVTVIAVEIEELTGKR
                     SGY"
     gene            710..868
                     /locus_tag="PLT69_05470"
     CDS             710..868
                     /locus_tag="PLT69_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13924.1"
                     /translation="MRIDMNLALPALFIVLGAADLAYGLYRHDAVSMVMGAAMVALAA
                     WIIFQRKT"
     gene            959..2146
                     /locus_tag="PLT69_05475"
     CDS             959..2146
                     /locus_tag="PLT69_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HOJ13925.1"
                     /translation="MGERRQGRGTMSVWAGEGRSSGGAPTQIPIVQSVSFGYDDMDAW
                     MRVGRGVEPGHIYSRNTNPTVEAFEEKVRALEGAEAATAFGSGMAAISNTLFTLLSPG
                     DRVVSVKDSYGGTSKLFMEFLPRFGIEAVLCDTTDHAMIEGELKRGCRLLYLESPTNP
                     TLKVIDIARLASSAHECGAVVVVDNTFATPINQRPLELGADLVIHSATKFLGGHADAL
                     GGVVCGPRALVKEIFHFREINGAALDPMAAYLLLRGMKTLHLRVARQNESALAIARWL
                     TGHRAVREVFYPGLETHPGHSIAASQMKGFGGVLSFSLAGDIEAVKVFLPRLKLAHRA
                     ANLGAVETIAGPPATTSHVECTPEERFAMGIPEGLVRYSVGIEDVEDLIGDLAQALEG
                     IPA"
     gene            2253..3149
                     /locus_tag="PLT69_05480"
     CDS             2253..3149
                     /locus_tag="PLT69_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ13926.1"
                     /translation="MALSFRPARTQDLEAVFDVYRESLEDVYVRHGFDPPHIHGKVNP
                     FHAFCLESEPGGFFVAEEEGEIVGASLGWARGRLWFLSHLFVRPHMQRRGIGGTLLEL
                     ARTHAGESGADIEAVVTMAFNPVSVGLYLGTGMLTVTAVLLMKMHGRPAPDGVTNEDA
                     GGVHETRPEVPVLEFLDAETLGFSRGSQHRFLLGLEGAGCTTVLRGTTPAAYAYHFAD
                     GRIGPACALDEDSLERALRAATAQAAGVAPTLSLMVPTSNRRALKTAVSLGFRPDVPY
                     VLLSSRPFGSFERYLLHSPGMM"
     gene            complement(3397..>4354)
                     /locus_tag="PLT69_05485"
     CDS             complement(3397..>4354)
                     /locus_tag="PLT69_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HOJ13927.1"
                     /translation="TADNVDDLETIVRLFRELGPPLLLKLDKGGGGKGIQPICTEDQI
                     VPVFESSKSIGKMAFGSPECYVEKRIANPRHIEVQCLGDNEGNYVALGERECSVQRKY
                     QKVVEEGPSPVVSVEDRQKLQSWAVSILREMGYNNAGTVEFLRDENGNFYFMEVNARI
                     QVEHPVTEMITGMDLVKTQLLIAMGERLGIRQEDVNIRGNAIELRIYAEDPFTFVPSP
                     GTVTEIAFPRLSNGVRLDHALEPGLKVSPFYDPMLAKLIVHAADRRGAIELMKESLRA
                     FRIEGVKTTIPLGLLIMESDAFVEGSFTTDTLAGLIEAATTR"
BASE COUNT          754 a         1391 c         1467 g          742 t
ORIGIN      
        1 ctctacctcc acagcgcgcc agagggcagg aagatcgacc tcatcaggaa gaacccgaag
       61 gtctgcttcg gcgtcgagtc cacctgcgag gtggtcccct ccgacagggc atgctcgttc
      121 accatgcggt acgagagtgt cgtcgggaag ggaaccgcac ggatcgtcac ggacgaggcc
      181 gaaaagaggc gtgccctgga ggtcatcacc ggacggtacg cgcacgaggc gaccccgctt
      241 ccagagggcg ccaccgggaa ggtcacggtc atcgccgtcg agatcgagga gctcacgggc
      301 aagagatcgg ggtattgagg tcggggagag acgcggggat cgcgttcgtg tatactcggg
      361 ccctcgagac gggtgcccca cgccgggtgc caaaaaggcg ggtggctgca cgacccacga
      421 aggagcgctc caccgggaag gccatgccgg gcacgagggc ggctgccccg tcccgcacac
      481 ggcatggggg tgcagcgacg tcaggtcggc ccggcgcgcg gcactgcagg tcctcgagca
      541 gggatgcaag ccgcgggagg acgggcaggg gtctttgcat ggacaacccc ggccgtgcga
      601 tctccgggcc gtttcacgtc gccgggcggg ccggcgcacc ggtcacccgc ctggaggacc
      661 gttcccggca cacccggcac cggaggcgcg agccacggga ggaacgagca tgcgcatcga
      721 catgaacctg gcgcttccag cgctcttcat cgtcctcggg gcggcggact tggcctacgg
      781 cctgtaccgc catgacgcag tgagcatggt catgggggcc gccatggtcg cccttgccgc
      841 ctggatcatc tttcaaagaa agacttaaga caggaaaagg aaatcgtgat cgggcgggtg
      901 ggtatacgcc gtatcgcacg tcgcccgcga gaatgtccgg agagaaagga gatcatgcat
      961 gggtgagagg agacagggcc gggggaccat gtccgtgtgg gcgggagagg gacgatcttc
     1021 aggtggcgca cccacccaga tacccatcgt gcagtcggtt tccttcgggt acgacgacat
     1081 ggacgcctgg atgagggtcg gcagaggcgt cgagccaggc cacatctact cgaggaacac
     1141 gaacccgacg gttgaggcat tcgaggagaa ggtgcgcgcc ctcgagggcg cagaggctgc
     1201 taccgcgttc ggcagcggca tggccgcgat cagcaacacc ctgttcaccc tcctctcgcc
     1261 gggggatcgc gtggtgtccg tgaaggacag ctacggcggg accagcaagc tcttcatgga
     1321 gttcctcccg cggttcggca tagaggcggt cttgtgcgat accaccgatc acgcgatgat
     1381 cgagggagag ctcaagaggg gttgcaggct gctctacctc gagtcgccca ccaacccgac
     1441 cctcaaggtg atcgacatag cgcgcctggc ttcctcggcg cacgagtgcg gggccgtcgt
     1501 ggtcgtggac aacaccttcg cgacacccat caaccagagg cccctggagt tgggggcgga
     1561 cctcgtcatc cacagcgcca ccaagttcct gggcggccat gcggacgcgc tgggaggggt
     1621 ggtctgcggg ccgagggccc tcgtgaagga gatcttccac ttcagggaga taaacggcgc
     1681 cgccctcgat cccatggccg cctacctgct gctcaggggc atgaagacgt tgcacctgag
     1741 ggtggcgagg cagaacgaga gcgcgctggc tatcgccagg tggctcacag ggcaccgggc
     1801 tgttcgcgag gtcttctatc cagggctcga gacccatccc ggccactcga tcgccgcgtc
     1861 ccagatgaag gggttcgggg gggtgttgag cttctcgctc gcaggcgaca tcgaggcggt
     1921 gaaggtcttc ctgcccaggc tcaagctcgc gcacagggcg gcgaacctcg gcgccgtgga
     1981 gacgatcgca gggcccccgg ccacgacgag ccatgtcgag tgcacacccg aggagcgctt
     2041 tgcgatgggc atacccgagg gcctcgtcag gtactcggtg ggcatagagg acgtggagga
     2101 cctcataggc gatctcgctc aggcgctgga aggcatcccc gcgtgaggat gcggcgtgag
     2161 gcgtgcatcg tgtagacacc gcctgtgaac ctgtataaga aagcaggaag gggcgccgcg
     2221 tccacgcggg gacgaaaacc aggggggttc ggatggcact atcctttcgc ccggcccgca
     2281 cgcaggacct cgaggcggtc tttgacgtct acagggagtc tctcgaggac gtctacgtgc
     2341 gtcacgggtt cgacccgcct cacatccacg gcaaggtgaa cccgttccat gcattctgcc
     2401 tggaaagcga gccgggcggg tttttcgtcg cggaagagga aggagagatc gtgggggcct
     2461 cgctggggtg ggcgagaggc cggttgtggt tcctctccca cctgttcgtc aggccgcata
     2521 tgcagaggcg tggcatcggg ggcaccctgc tggaactcgc gaggacacac gccggggagt
     2581 cgggcgccga catcgaggcg gtggtcacca tggcgttcaa ccccgtatcc gtgggactgt
     2641 acctgggaac aggcatgctc acggtcacgg cagtgctgct catgaagatg cacggacggc
     2701 ccgcgccgga cggggtcacg aacgaggacg cggggggtgt tcacgagacg cgtcccgagg
     2761 tccctgtcct ggagttcctg gacgccgaga ccctggggtt ctcgagggga tcacagcacc
     2821 ggttcctgct gggtctcgaa ggggccgggt gcacgacggt cttgagaggc acaaccccgg
     2881 ccgcgtacgc ctaccacttc gcggacggcc gcatcgggcc cgcctgcgcc ctcgacgagg
     2941 actcgctcga gagggccctg cgtgcggcca cggcccaagc ggccggggtt gcacccaccc
     3001 tctccctcat ggtgcccacg tcgaacaggc gtgccctgaa gaccgcggtc tccctcgggt
     3061 tcaggcccga tgtcccctac gtgctcctct cctccaggcc gttcggttcc ttcgaacgct
     3121 atcttctcca ctcgcccggc atgatgtgac ccgcaccacg ccggacggac cgccggagac
     3181 gaccgtcatc gtcccgaccg ggtcttgcag ccgagccagg cttgtgcctg agagaccggc
     3241 cgtgttccat cgctcccata tccgcgtggc gtccacccgt tctctccgct cgcgggtgcg
     3301 tcaccggagg ggtgttcgca ccacgctctc gggccggatc gtgaccccac ctctttctcg
     3361 gcggctctta cccggccacg acaagggagc gtcccgtcac ctcgttgtcg ccgcctctat
     3421 gagccccgcc agcgtgtcgg tagtgaagct gccttccacg aaggcgtccg actccatgat
     3481 caggagcccg agcggtatgg tcgtcttgac cccctcgatg cggaaggccc tcaggctctc
     3541 cttcatgagc tcgatggccc cgcgcctgtc ggctgcgtgg acgatgagct tggccagcat
     3601 ggggtcgtag aagggggaga ccttgaggcc cggctcgagc gcgtggtcga gcctcacgcc
     3661 gttcgacagc cgggggaacg cgatctcggt cacggtgccc ggagacggca cgaaggtaaa
     3721 ggggtcctcg gcatagatac gcagctctat ggcgttcccc cgtatgttca cgtcttcctg
     3781 gcggatgccg agcctctcgc ccatggcgat caagagctgg gtcttgacca ggtccatccc
     3841 ggttatcatc tcggtgaccg ggtgttccac ctggatccgt gcattcactt ccatgaagta
     3901 gaaattgccg ttctcgtccc gcaagaactc caccgtgccg gcgttgttgt accccatctc
     3961 cctgagtatg ctcacggccc aggactggag cttttgccgg tcctcgaccg agaccacggg
     4021 agaaggaccc tcctcgacga ccttctggta cttgcgttgg acggagcact cgcgctcgcc
     4081 cagggccacg tagtttccct cgttgtcacc caggcactga acctctatgt ggcgtgggtt
     4141 ggcgatgcgc ttctcgacat agcactcggg tgaaccgaag gccatcttcc cgatgctctt
     4201 cgacgactcg aagaccggga cgatttggtc ctcggtgcag atgggctgaa tgcctttccc
     4261 tccgcctccc ttgtccagct tcagaaggag cggcgggccg agttccctga acagccttac
     4321 gatcgtctcc aagtcatcga cgttgtcggc cgtg
//
LOCUS       DAOSCR010000172         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12696_length_4350_cov_3.432363, whole genome shotgun sequence.
ACCESSION   DAOSCR010000172 DAOSCR010000000
VERSION     DAOSCR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12696_length_4350_cov_3.432363"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLT69_05490"
     CDS             complement(<1..321)
                     /locus_tag="PLT69_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ13928.1"
                     /translation="MNVKEMFDLKGKVAVVTGGGRGLGRFIAQGLAEAGSDVVLASRK
                     VANCEKAASELASLGVKTLAVKCDTAVEEDIQNLVDATMKTFGTIDILVNNAGITWGA
                     PAENH"
     gene            complement(356..1460)
                     /locus_tag="PLT69_05495"
                     /pseudo
     CDS             complement(356..1460)
                     /locus_tag="PLT69_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463566.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
     assembly_gap    1461..1470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1471..1829)
                     /locus_tag="PLT69_05500"
     CDS             complement(<1471..1829)
                     /locus_tag="PLT69_05500"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOJ13929.1"
                     /translation="MDSSKLGRSMYELMMRTSTELPEDVTCALEKAREREPVHSTAAN
                     TLDIILKSAELSRANRLPICQDTGVPFAVVRAPQAVDTAEITRALVSALKTLTIEGVL
                     RQNCVDPVNGSNSGDNTG"
     gene            complement(2178..2843)
                     /locus_tag="PLT69_05505"
     CDS             complement(2178..2843)
                     /locus_tag="PLT69_05505"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOJ13930.1"
                     /translation="MTGAVLIGGQSKRFGTDKVLSRLNGLMLVEHVVHVLQTVFDEVI
                     LVGHARPGLEGYTVVQDIRPGLGPLGGIYTALTAARSDRCFVCAADMPNLDAAFISHI
                     AALSEGYEIVMPVWSQGREPLHAVYRKTLLQAIERLLEAGQRSIYALAQQANTLFVTE
                     ETIRLYGDPSTMFANINTPHDMKTFTAGSVQEAIQGRPEHGAQPAARSTTSNPATAVR
                     KGT"
     gene            3442..3948
                     /locus_tag="PLT69_05510"
     CDS             3442..3948
                     /locus_tag="PLT69_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ13931.1"
                     /translation="MSAVEKSKTDPGLAEFTAEQLAQVDKIIEANKGKPGSLIPVLEQ
                     VQEVCGYLPAAIQKVVSEGLNIPLANVFGVVTFYSFFTMVPRGKHVIRVCLGTACYVR
                     GSQGLLDRLTHILGIEPGGCTEDRNFSLETVRCLGACGLAPVVVIDSDTHGQVKAAKL
                     EQVLAKYN"
     gene            3958..>4350
                     /locus_tag="PLT69_05515"
     CDS             3958..>4350
                     /locus_tag="PLT69_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ13932.1"
                     /translation="MAKLTIEDLKKIKERVEREDQFRAGSKRVKITVHMGTCGIASGA
                     KEILDTFMNEISESNATEVMVTTSGCIGICSREPLATVEEQGKDPIVYEYLTPDKARQ
                     IFKQHILGGVVQTEFVMAKGSEHDTKKGV"
BASE COUNT          781 a         1343 c         1385 g          831 t
ORIGIN      
        1 gtggttctcg gcgggcgccc cccaggtgat gccggcgttg ttcacgagga tgtctatggt
       61 gccgaaggtc ttcatggttg catccaccag gttctggatg tcttcctcga cggccgtgtc
      121 gcacttgacg gcaagggtct tcacgccgag gcttgcaagc tcggatgccg ccttttcgca
      181 gttggcgacc ttccgggacg caagcaccac gtccgagccc gcctcggcca agccctgggc
      241 gatgaatctc ccgaggcccc ttcctccacc ggtgaccacg gccaccttgc ccttcaggtc
      301 gaacatctct ttcacgttca tctgcatacc ctccttgttg atggtgttgt ccctctcagg
      361 tgcgaggggg tgtgagcgtg ctctcgagca ccctcttgag catcgacgcc gacctgtcga
      421 ggatgtgccg gtgcagcgag gcgcccttgc cgtccatggt gacgagggcc ggcaagtccc
      481 tcacgcagag cacccacatg gcctcgggcc ggccgaactc ctcgaggtac accccctcga
      541 cgcgctcgat gcacgatgcg aggtagacgg ctgcgccgcc tgtcgcgtgg aggtagggga
      601 tcccgtgctc gacgagcgcc ttcgacgtgc gttcccccat ccctcccttt cccatgacgg
      661 cccccggctt gaaccgggct atgacgtccg cctggtacgg ctcctctctc atggaagtgg
      721 ttggccctgc tgcgacgacc ctgtgagagc ctgcatcccc gacgacgatg gggccgcagt
      781 gatagagcac catgcctgtc aagtccaagg gggactcgcc tcctgaggcc aggtacctgt
      841 gcacctggtc tcggccggtc acgatcctcc ctgagacgag cacggtgtcg cccacggcga
      901 gcgacgcggc tgtctcaggg tccatgggcg agcgtatgcg cctcagagcc atctcgcgac
      961 gccccctgtc tcgtcgagtt ccacgctgta cctcctcgtc gcccagcacg agtagctcac
     1021 ggtgacgaag aaactggccg ggtgcctccc tgcggcgttc agcgcgcaac cgaggagggt
     1081 cgtgttgccg cccaggccca tgggccctat gcccagcatg ttcgcctctt cggtgatcac
     1141 ggactcgagt ctcgccaggt ccacgtcggg gtttgcctcg ccgagcttcc tgaagaggcc
     1201 tttctttgcc acgagatagc cggacgcgcg gtcgccccct atgcaaacgc tgatgaagcc
     1261 aggcgcgcac cctctcccct gggccttgaa gaccgcatcc agcacgcacc tcctcacgcc
     1321 ttcgaggtcc ctgcctgccc gaagctcgtc gcatgggagg ctgtactggg tgctcacgtt
     1381 ctcggacccc cctcccttga gcatgaaatc cactctgatg gggccttcgg agggctcgta
     1441 gtggacctga gggacccgca nnnnnnnnnn ccggtgttgt ccccggagtt ggacccgttg
     1501 accgggtcca cgcagttctg tctcaagacg ccctcaatgg tgagggtctt caaggcggag
     1561 acgagcgctc tcgtgatctc ggccgtgtcg acggcctggg gcgcccggac gaccgcgaac
     1621 ggcacgcccg tgtcctggca gatgggcagg cggtttgcac gcgaaagctc cgcgctcttg
     1681 aggatgatgt cgagggtgtt tgcggccgtg gaatgaacgg gttccctctc cctggccttc
     1741 tcgagcgcgc aagttacgtc ttcgggcagc tcggtgctcg tgcgcatcat gagttcgtac
     1801 atgctcctgc ccagcttcga gctgtccatg gggatcatat accataccga ctcctggcag
     1861 gccagcgcct ctggtgtcca ctccccccgt ggtctcgagc ggctccagcc aacggtcaag
     1921 ggacatggcc ttccgagagg gcgggcaaat ccttgaacaa gggtgcatcg accgtgggcg
     1981 caggtcgcaa ggtcgtcacg ccttaggccg accggcaggc cgtgcacggt atccgggccg
     2041 tagaggtgcg cagcgcaccc ctggggacgg gagggtgtcc tcgagcatgg cggcctgcgg
     2101 gttttcttgc ctgtccttgt gcttgcctcg gcttgccacc tcagggacga tcggctcggg
     2161 catggcggcc tacgggctta cgtgcctttc ctgacggctg ttgccgggtt cgaggtcgtt
     2221 gatcgtgcgg cgggctgggc gccgtgctcc ggacggccct ggatcgcctc ttggacactg
     2281 cccgcggtaa aggtcttcat gtcgtgaggg gtgttgatgt tggcgaacat ggtggagggg
     2341 tcgccgtaga gcctgatggt ctcctcggtg acgaagaggg tgttggcctg ttgggcaagg
     2401 gcgtagatgc tccgctgccc ggcctcgaga agccgttcaa tggcttgcag caaggtcttc
     2461 cggtagaccg cgtgcagtgg ctctctgccc tgggaccaca cgggcatgac gatctcgtac
     2521 ccctcggaaa gggccgcgat atgggagatg aaggcggcgt ccaggttcgg catgtcagcg
     2581 gcgcagacga agcaccggtc gcttcgtgcg gccgtcaggg cggtgtagat gccgccaagc
     2641 ggacccagcc ccgggcgtat gtcctggacg acggtatacc cctcgaggcc gggccgtgca
     2701 tgtcccacca ggatgacctc gtcgaagacg gtctgcagga catgcacgac gtgttcgacg
     2761 agcatcagcc cgttgagcct cgaaaggacc ttgtccgtgc cgaacctctt cgattgtccg
     2821 ccaatgagca cagcgccggt catggtaaga gcttgtagat tgtatcggtg caagagtcaa
     2881 tcgatctcgg catcgtcgtt ctgccgccct acctgccacg gttcatactg tgtaccggga
     2941 aatcccccgt acccgctcga gcgccaccaa cccggctcgg tgtgcgtaac cgggaaggga
     3001 cacaagacat catgcaggga agcagtacaa gggctggatc acgcctcgaa ggaactcgaa
     3061 ggaacatgca ggggttctcg agcgaagagg ctcgggcgac ggtcccgccg gaggtcctcg
     3121 tacaccgttg gcacccatgc cctttatcct gcccgttctc ctggacacat gcctgccgac
     3181 gaccttctct ataggaaaag aacgatcatt tttccgtata tgattgggcg aagcatcacg
     3241 tgttcaatgc acgatgaagg atgatccttg ccgcatcgag gaacaggcga taccctattt
     3301 tcttgcttct ttcgacatcg cgaactgaat catgaaccac cattcagttg acccttgaag
     3361 cgggccttga agcatgataa ggaaagagag ccgtaaggca tgtcgcacca actcgaaaga
     3421 ctacgcgata gggggttgac cgtgtcagca gtcgagaaga gcaagacgga tccgggtctc
     3481 gcagagttca cggcggaaca gctcgcgcag gtggacaaga tcatcgaggc caacaaggga
     3541 aagccggggt ctctcatccc cgtgctcgag caggtccagg aagtgtgcgg gtaccttcct
     3601 gcggcgatcc agaaggtggt ctccgaggga ctcaacatac ctctggccaa cgtgttcggc
     3661 gtggtgacgt tctattcgtt cttcaccatg gtcccgaggg gcaagcacgt gatccgggtc
     3721 tgcctcggga cggcgtgtta cgtcaggggc agccaaggtc tcctcgatag gctgacacac
     3781 atcctcggca tagagccggg cgggtgcacc gaggacagga acttctcgct ggagacggta
     3841 aggtgcctgg gcgcatgcgg gctcgccccg gtggttgtca tcgattccga cacccacggc
     3901 caggtcaagg ccgcgaagct cgagcaggtg ctcgcgaagt acaactagga gggatccatg
     3961 gccaagttga cgatcgaaga tctgaagaag atcaaggaac gcgtggaaag ggaagaccag
     4021 ttcagggccg gttcgaagcg cgtcaagatc acggtccaca tgggcacctg cggcattgcg
     4081 tcgggagcca aggagatcct cgacaccttc atgaacgaga tcagcgagag caacgcgacc
     4141 gaggtgatgg tgaccacgtc cgggtgcatc gggatttgca gccgcgagcc cctcgcgacc
     4201 gtcgaggagc agggcaagga tcccatagta tacgagtacc tgaccccgga caaggcgagg
     4261 cagatcttca agcagcacat cctcgggggc gtcgtccaga cggaattcgt catggcgaag
     4321 ggctcggagc acgacacgaa gaagggggtt
//
LOCUS       DAOSCR010000173         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12697_length_4350_cov_3.376019, whole genome shotgun sequence.
ACCESSION   DAOSCR010000173 DAOSCR010000000
VERSION     DAOSCR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12697_length_4350_cov_3.376019"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(526..1728)
                     /locus_tag="PLT69_05520"
     CDS             complement(526..1728)
                     /locus_tag="PLT69_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ13933.1"
                     /translation="MAKHNSGFPLFKSRAFSYQALRTMGHACAFGATLGECLGAVRTI
                     RENDGESWFAAWGKAAVMCEKLSVSEGDRISRGKALLRAANYYRASEFFLPASDPRRR
                     ETYTRETDAFNRALKALGIPYHAWNVPYERVSMRAYYFPGDPSLPVIMACGGFDSSLE
                     ELFFWIGHASIERRHPCILFEGPGQSSMIREYGLTFIPEWEKPLMILLDHAQWVAPEI
                     SQGQKIIAGVSMGGMLALRAASRDKRIHAAACLGGFFSMETAAFERLPRAARWAYRLG
                     MKNTFDNLAHFAASRDMERKWALEHGMWVLGASSPSDLLEKTADFSLAPAAKDITCHV
                     LVVLGQNDHLVPATEGRVFQKHLTQAKSLKVVTLDTSTGAGEHCQAGATERLHQVFFG
                     WAEGISGR"
     gene            complement(2062..2946)
                     /locus_tag="PLT69_05525"
     CDS             complement(2062..2946)
                     /locus_tag="PLT69_05525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018691411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ13934.1"
                     /translation="MVDLSTFRGVAALTLMFLNTLFWAIPVHLLALTKLVIRSSSWQV
                     TCAKCLMKTVNCWIWGILTALRLTQRTVYDISGRENLSTSEWYFINSNHRSWADILVL
                     LVSLHGYIPFPKFFLKRELFKIPILGTAWWALDYPFMERYSKEFLEKNPHLKGKDLET
                     TRRMCERYKHTPVSILNFIEGTRFTPAKHERQKSPYRHLLKPKAGGFAFALAAMDGKI
                     RKIIDVTIIYPKRNFTFWDYLCGRVDRIRVIVKTLSVPDEFLYGDYENDASFRERFQS
                     WVTNLWEEKDRLLDEHLC"
     gene            3608..4350
                     /locus_tag="PLT69_05530"
                     /pseudo
     CDS             3608..4350
                     /locus_tag="PLT69_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
BASE COUNT          786 a         1231 c         1487 g          846 t
ORIGIN      
        1 cctccgctct atggtctgga atctgcaggg ttgatacagg caacaagcca gggtgtcaac
       61 aggagatttc ccgccgtgag acggactgcg tgccacggcg gcgggcctca tggggggcga
      121 cgggagcgcg gcctgaggga tcgagagtgc ctgtcgcgtc cggcctcagg ctgctcgcgc
      181 catgccctgc ggggagtgag cggccatgct cgatccaggc ggcgcacggc cgagccgaca
      241 ccgggcacgt gccgaaggga acgaggatgg tgtcttggcg atgacgctaa aggttcaggg
      301 taaacaagca ggccttggcc cggtatctgg gctgtgaacg aggatggtgt cttggcgagg
      361 acgcccgagg gagggaaggg aggcggggtg cgaggtgtcg atgggggagc atcgagacga
      421 aacccccggt accgttccag cagcggtcaa ggacgcggcg atgcgcgcgg ccgcaccggc
      481 ccggaccagt ccgaggcagt gtctcggtga tcctgcggta tgcccctacc tgcccgagat
      541 gccctcggcc cacccgaaga acacctggtg gagtctttcc gtggcgcccg cctggcagtg
      601 ttcgcccgcc cctgtggatg tatcaagggt gaccaccttc aggctctttg cctgcgtgag
      661 gtgcttctgg aagaccctgc cttccgtggc cggcacgaga tggtcgttct ggccgagcac
      721 gaccagcacg tggcaggtga tgtctttcgc cgcgggtgcg agagaaaagt ccgcggtctt
      781 ctccaggaga tccgaagggg acgatgcgcc cagcacccac atcccatgct cgagggccca
      841 cttcctctcc atgtccctcg aggccgcgaa gtgggcaagg ttgtcgaacg tattcttcat
      901 gcccagacgg tacgcccacc gggccgcacg ggggagcctc tcgaatgccg cggtctccat
      961 ggagaagaac cccccgaggc aggcggcggc gtggatcctc ttgtccctgg aagcggccct
     1021 gagcgcgagc atgccgccca tgctcacgcc ggcgatgatc ttctggccct gcgagatctc
     1081 gggcgccacc cactgtgcgt ggtccaggag gatcatgagg ggcttttccc actcggggat
     1141 gaaggtgagc ccgtattccc ttatcatgga cgactgcccc gggccctcga acaggatgca
     1201 cgggtgccgc ctttcgatgg atgcgtgccc gatccagaag aacagctcct cgagggagga
     1261 gtcgaaaccg ccgcatgcca tgatcacggg cagggaaggg tcgccgggga agtagtacgc
     1321 cctcatggag acgcgctcgt aggggacgtt ccacgcatgg tagggtatgc ccagtgcctt
     1381 gagtgcgcgg ttgaaggcgt ccgtctcgcg cgtgtacgtc tcacgcctac gcgggtcgga
     1441 agcgggcagg aagaactcgc tcgcgcggta atagttcgct gccctgagga gggccttgcc
     1501 cctgcttatc cggtcgccct ccgataccga gagcttctcg cacataacgg cggccttgcc
     1561 ccaagcggcg aaccagcttt ccccgtcgtt ttccctgatg gttcgcacgg cgccgagaca
     1621 ctcgccgagg gtcgcgccga atgcgcaggc atggcccatg gtcctgagcg cctggtacga
     1681 gaaggccctg gatttaaaga gagggaaacc cgagttgtgc ttggccatgg aacccccctg
     1741 ggtgtgcttg cggtgtagac ggagtctacc atggaaacgc gatggagatc aaagaaaaca
     1801 aggcggtacg acgggtctcg gggaataccc atagagacgt tccttcgtgc ccgggccgcg
     1861 tgcagcaagg cggctgcgct cacgcggtac ggatgggaca aaaccaggcg cttcggcatg
     1921 gttcgtttgt cgggcgaacg tcggggagca tgtccgacct gcggtctatg gtgcgacatt
     1981 gcttcggaaa gccccgaaca agggccgcaa ggtgaaagac tttggcgagg tattctcgtg
     2041 gtgccggtcc agcaagaccc atcaacagag gtgttcatcg aggagcctgt ccttttcctc
     2101 ccagaggttc gtcacccatg actggaacct ctcccggaaa gaggcgtcgt tctcgtaatc
     2161 cccgtaaagg aattcatcgg gtacggacag ggtcttcacg atgacccgta tcctgtccac
     2221 cctgccgcag aggtagtccc agaaggtgaa gttcctcttg gggtagatga tcgtcacgtc
     2281 tatgatcttc ctgatcttgc cgtccatggc ggcaagagcg aaggcaaacc cgccggcctt
     2341 cggtttcagc aggtggcggt acggggattt ctgtctctca tgcttggcgg gcgtgaagcg
     2401 ggttccctcg atgaagttca ggatggatac gggggtgtgc ttgtatcgtt cgcacatcct
     2461 cctggtcgtc tcgaggtcct tgcccttgag atgcgggttc ttttcgagga attccttcga
     2521 gtacctctcc atgaaggggt agtcgagcgc ccaccatgcg gtccccagta tgggtatctt
     2581 gaagagctcc ctcttcagga agaatttggg aaaggggatg tacccgtgca aggacaccag
     2641 caggacgagg atgtccgccc acgaccggtg gttgctgttg atgaagtacc actcggacgt
     2701 gctcaggttt tcgcggccgc tgatgtcgta cacggtcctc tgggtgagcc tgagcgccgt
     2761 gaggatcccc catatccagc aattgacggt tttcatgagg cacttcgcgc acgtgacctg
     2821 ccatgagctg gacctgatca cgagcttcgt gagggcgagc aggtggacgg gtatggccca
     2881 gaagagcgtg ttcaggaaca tgagggtcag ggcggcgacg cccctgaatg tcgatagatc
     2941 aaccatggtg tgtgagcggc ttcatgttct catgctgtgt gaaccatacc tctgggtgcc
     3001 tgcagccgac cggtctcggg aggatcgcgc cccttgcggc atggaggctt cacctgcgtg
     3061 acagcatcct gcccgtcttc cgttcatctc ggcacggccg gccgattcat accgggatgc
     3121 ggcctttgag gagcacccgt tcgtgccggt tacgatgcag ccatggattg tatgaccggc
     3181 tgacgagaaa agcaacgagg aggtgcatag tgtcatgtgt tttaacgata tttttacaag
     3241 cggtcgggtg cgtgggctgg tgccgagacg ctattcggtc ttgccatggg ggccgggaat
     3301 aaatataacg gtgttcatgt aacggaaaac ggccttgtgt actcatgggg cggcgcgtgt
     3361 ttcgctgtcc ccgtgggaaa cccggtcgcg gaatcgatgg gtcgtggggc cgaattcccc
     3421 tgtgcatcgc ggacgagacg ggagatggcc cttgtcggcg tgtctgtaga cgcctgttgc
     3481 gtcctcgtga tgctttctcg tcacgggagg aacgtgtctc ggcgatcagc gggtgtacgg
     3541 gggctggaga ccatcgagcg ggagattcgt tgcaagacga ggggagggtg acatgggata
     3601 cggttacatg ggaagggtcc tgaatgtcga tctgggcgcc gggagcgtgg aggtcgagcg
     3661 catacccgac gaggtctacg ggaagtatct ctcggggctc ggcctcgggt cgtacatcct
     3721 ctacaacagg atccccaggg gtgcagaccc gctcgggcca gacaacgtcc tcggtttcgt
     3781 gtccgggctt ctcaccggga ccggcagcct gttcgcgggc aggtggatgg tggtaggcaa
     3841 atcacccttg acgggtggct ggggcgatgc caactgcggg gggatgtttt cgccggccat
     3901 caagcgctcg ggtttcgacg gcatcttctt caagggtgta agcgaaaggc ccgtgttcct
     3961 ctccgtgaag aacggccggg ccagataagg gacgcggccg atctctgggg caaggacacg
     4021 atcaccacgg agagcatcct gaaaaaggaa ttcggcgagc acgtgtgtgt ggcctgcata
     4081 gggcccgcag gcgagcgact ctcgctcata tccggcgtgt gctgtgacca ggggaggatg
     4141 gcggcccgca gcgggctcgg cgcggtgatg gggtcgaaga ggctcaaggc tgttgtgctc
     4201 gacggcaagg cgcgtatccc cgtctacgac agggaccagg tccacaggct gagcggtagg
     4261 tgcggcaggt gggtgaggtt ccagctcccc ctgggcccgg gcaccctcat gggatacctc
     4321 gggaccctgc tcagggtgct gccgacggcg
//
LOCUS       DAOSCR010000174         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12730_length_4340_cov_3.441074, whole genome shotgun sequence.
ACCESSION   DAOSCR010000174 DAOSCR010000000
VERSION     DAOSCR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12730_length_4340_cov_3.441074"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /gene="ribD"
                     /locus_tag="PLT69_05535"
     CDS             complement(<1..322)
                     /gene="ribD"
                     /locus_tag="PLT69_05535"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOJ13935.1"
                     /translation="MADARDAEFMREAVHLAGQALGRTAPNPSVGAVVVKDGRVVGRG
                     FHPKAGMPHAEIYALKEAGERARGATVYVTLEPCNHYGRTPPCTEALIKAGVKRVVVG
                     SLDPN"
     gene            complement(392..859)
                     /gene="nrdR"
                     /locus_tag="PLT69_05540"
     CDS             complement(392..859)
                     /gene="nrdR"
                     /locus_tag="PLT69_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOJ13936.1"
                     /translation="MRCPRCQGLDDKVLQSRLSQDGKAIRRRRQCERCSHRFTTYERI
                     EEIVPVVVKKDGRREPFDAAKLVAGVRTACGKRPVGEEQIEALKDAIVQDIQARWDRE
                     VPSSYIGEKVMEGLHRLDQVAYVRFASVYREFKDVSEFMDEIKGLKRARKPRR"
     gene            complement(865..2109)
                     /gene="glyA"
                     /locus_tag="PLT69_05545"
     CDS             complement(865..2109)
                     /gene="glyA"
                     /locus_tag="PLT69_05545"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOJ13937.1"
                     /translation="MNLEHVKRTDPDVYNAIMGELSRQRDKLELIASENIVSEAVLEA
                     QGCVMTNKYAEGYPAKRYYGGCEYVDVVEELAIERAKELFKAEHANVQPHSGSQANMA
                     VYFSVLEPGDTYLGMNLAHGGHLSMGSPVNFSGRIYKVVPYGVRPDTHLIDYDQLRDL
                     ARKHRPKLVIAGASAYPRIIDYGIFKEVADEVGAVFMVDMAHIAGLIACGLHPSPVPL
                     ADFVTTTTHKTLRGPRGGMILCKEQHAKTLNSKVFPGMQGGPLMHVIAAKAVALKEAL
                     SPEFREYQAQIVKNAKAMAEEFLALGFRLVSGGTDNHLMLIDLTNKDVTGKEAQEMLD
                     AANITVNRNAIPFDTKSPFVTSGIRIGTPALTTRGMKEEEMRFVAGLIARVIDAKGDP
                     QVIESVKKEVSGLTRRFPVYGG"
     gene            complement(2357..2797)
                     /gene="rpiB"
                     /locus_tag="PLT69_05550"
     CDS             complement(2357..2797)
                     /gene="rpiB"
                     /locus_tag="PLT69_05550"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ13938.1"
                     /translation="MRVAVACDHGGVTLKPAVVEELAARGAEVLDLGTHGTQSVDYPD
                     FARLAVEAYIARECERIVLICGTGIGMSICANRVPGIRAALCHDEYTARMSRLHNDAN
                     CLVLGGRVIGPGLARAIVGVWMDTPFEGGRHEARVKKIEDMPGS"
     gene            complement(2794..4029)
                     /gene="fabF"
                     /locus_tag="PLT69_05555"
     CDS             complement(2794..4029)
                     /gene="fabF"
                     /locus_tag="PLT69_05555"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOJ13939.1"
                     /translation="MKKRVAVTGIGVVSPLGCDCTTLWDNLTNGVSGIEPIDELAGLP
                     VTFGGRARGFDPGKYLGPKEIRRMDRYAQMAVTCALDAWAQSGLGRNDYPAHRVGVLV
                     GTGAGGISTLEEQIPVYVEKGPRRVSPFTVPMFIPNIASAEVSMKLKAQGPGMGLSTA
                     CATGGHALAVAARLIQAGDADCMVAGGVEACLTPFSISAFASMRALSTRNDEPSRASR
                     PFDRDRDGFVMAEGGAVFVLEDREAARARGAQILGEIMGAGMSQDAYHITAPEPEAQA
                     VVFAMERALEDAGVSPGDIGYINAHGTSTQLNDVTETLAVKKVFGGLAYRIPMSSTKS
                     MTGHLIGGTAGIETAVCILAMRNGVIPPTINLDNPDPQCDLDYVPHQARPAEIACCLN
                     NSFAFGGQNVVLVLGRGER"
     gene            complement(4039..4272)
                     /gene="acpP"
                     /locus_tag="PLT69_05560"
     CDS             complement(4039..4272)
                     /gene="acpP"
                     /locus_tag="PLT69_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ13940.1"
                     /translation="MADVQQRIIQLIAKQLEKDPSEIKPEMSFADDLGADSLDLVELI
                     MAVEEEFNIEVPDEDAEKIKFVKDAIEYVKSKI"
BASE COUNT          627 a         1530 c         1349 g          834 t
ORIGIN      
        1 ggttggggtc gaggctcccc acgacaaccc gcttgacacc cgcctttatg agcgcctcgg
       61 tgcagggggg cgtcctgccg tagtggttgc acggctcgag cgtcacgtag acggtcgcgc
      121 cgcgggcgcg ctcccccgcc tccttgaggg cgtagatctc cgcgtgcggc atgccggcct
      181 tcgggtggaa ccccctgccc accacgcggc cgtctttgac cacgaccgcg ccgacgctcg
      241 ggttgggagc ggtccttccc agggcctgcc cggcgaggtg taccgcctcc ctcatgaact
      301 cggcgtcacg cgcgtctgcc atgctcaccc cgcggcacga ggccgtggcg tctccggcgc
      361 gccccgtggg cgcccacgcc tgccacgcgc ctcacctcct cggcttcctc gcccgcttga
      421 ggcccttgat ctcgtccatg aactcgctca cgtccttgaa ctcgcggtat accgaggcga
      481 agcgcacgta tgcgacctgg tcgagcctgt ggaggccttc catcaccttc tcgccgatgt
      541 aggaggacgg cacctcccgg tcccacctgg cctggatgtc ctgcacgatg gcatccttga
      601 gggcctcgat ctgctcctcg ccgacgggcc gcttcccgca ggcggtcctc acgccagcga
      661 cgagcttggc cgcatcgaag ggctcccgcc tcccatcctt cttgaccaca acggggacga
      721 tctcctcgat cctctcgtag gtggtgaagc ggtgagagca cctctcgcac tggcgccttc
      781 gccgtatcgc cttcccgtcc tggctgaggc gggactggag gaccttgtcg tccagtccct
      841 ggcaccgcgg gcaccgcacc gctctcaccc cccgtacacg gggaatcgtc tcgtgaggcc
      901 ggagacttcc ttcttcaccg actcgatcac ctgggggtcc cccttcgcgt cgatgacccg
      961 ggcgatgagc ccggcgacaa accgcatctc ctcttccttc atccccctcg tggtgagcgc
     1021 aggcgtgccg atgcggatgc ccgaggtcac gaacgggctc ttggtgtcga acggtatggc
     1081 gttgcggttc acggtgatgt tggcggcgtc cagcatctcc tgtgcttcct tgcccgtgac
     1141 gtccttgttc gtgaggtcta tgagcatgag gtggttgtcg gtgccgccgg agacgagcct
     1201 gaaccccagc gcgaggaact cctcggccat ggccttcgcg ttcttgacga tctgggcctg
     1261 gtattccctg aactccgggc tgagcgcctc cttgagcgcc accgccttgg ccgcgatcac
     1321 gtgcatgagt gggccgccct gcatgccggg gaagaccttc gagttgagcg tcttcgcgtg
     1381 ctgctccttg cagaggatca tgccgcccct cggaccgcgg agcgtcttgt gcgtggtcgt
     1441 ggtgacgaag tcggcaaggg gcaccgggct cggatggagg ccgcacgcga tcagccctgc
     1501 gatgtgcgcc atgtccacca tgaagacggc ccccacctcg tccgccacct ccttgaagat
     1561 cccgtagtcg atgatcctgg ggtatgcgct ggcgccggcg atgacgagct tgggcctgtg
     1621 cttcctggcg aggtccctga gctggtcgta gtctatgagg tgcgtgtcgg gcctgacccc
     1681 gtaggggacg accttgtaga tcctgcccga gaaattcacc ggcgagccca tggagaggtg
     1741 tcctccgtgg gcgaggttca tgcccaggta cgtgtccccg ggctcgagca cggagaagta
     1801 cacggccatg ttggcctggg agcccgagtg gggctggacg ttcgcgtgct cggccttgaa
     1861 gagctccttg gcgcgctcga tggcgagctc ctccaccacg tcaacgtact cgcagccgcc
     1921 gtagtacctc ttcgccgggt acccctcggc gtacttgttg gtcatgacgc acccctgggc
     1981 ctcgagcacc gcctcgctca cgatgttctc ggaggcgatg agctcgagct tgtcccgctg
     2041 cctcgagagc tcccccatga tcgcgttgta cacatccgga tccgtccgct tcacgtgttc
     2101 aaggttcatc gtgttctcct gtgtatcgtc tctcgtgtgt cttctcaccc gtggcgaccg
     2161 tcccgcccca ggcctcttgg cgcccgctca cgggacctgc cgggtctctc aggcggcgcg
     2221 gcggcctggc gtcccgcctc cggccccttg tcacgagccc cagggaccgg ccgcgggcag
     2281 cgccgccctc ggccgtttcc cctcgcgatc cgtccccgct caggccaccc agggaaaggg
     2341 cgggggaccc gccgtgctac gacccgggca tgtcctcgat cttcttgacc cgcgcttcgt
     2401 gacgcccgcc ttcaaacggc gtgtccatcc acacgccgac gatcgcccgt gccaggccgg
     2461 gacctatgac ccttcccccc aacaccaggc agttcgcgtc gttgtggagc ctcgacatcc
     2521 gcgcggtgta ctcgtcgtgg cacagcgccg cacgtatccc cggaacgcgg ttcgcgcaga
     2581 tggacatgcc tatgccggta ccgcagatga ggacgatcct ctcgcactct cgcgcaatgt
     2641 aggcctcgac ggccaggcgg gcgaagtcgg gatagtccac ggactgggtg ccgtgggtac
     2701 cgaggtcgag tacctccgcg cccctggccg ccagctcctc gacgaccgcc ggcttcaggg
     2761 tcacgccgcc gtgatcgcat gcgacagcca ccctcaccgc tcgcccctcc cgagaacgag
     2821 taccacgttc tggccgccga aggcgaacga attgttcaaa cagcacgcga tctcggccgg
     2881 cctggcctgg tggggcacgt agtcgaggtc gcactgcggg tccgggttgt cgaggtttat
     2941 ggtgggcggg atcacgccgt ttctcatcgc aaggatgcag acagcggtct cgatcccggc
     3001 tgtgccgcct atgaggtgtc cggtcatgga cttggtcgag ctcatgggga tgcggtacgc
     3061 gagcccaccg aacaccttct tgaccgcgag cgtctccgtg acgtcgttga gctgggtgga
     3121 cgtaccgtgg gcgttgatgt agccgatgtc gccgggcgac acgccagcgt cttcgagcgc
     3181 gcgctccatg gcgaagacca cggcctgcgc ctcgggctcg ggggcggtga tgtggtaggc
     3241 gtcctggctc atgccggcgc ccatgatctc gcccaggatc tgtgcgcccc tggcgcgcgc
     3301 cgcctccctg tcctcgagca cgaacacggc gccgccctcg gccatgacga acccgtcccg
     3361 gtcgcggtcg aacggcctcg aggccctgga cggctcgtcg ttgcgggtcg acagcgcccg
     3421 catggaggcg aacgcgctta tcgagaacgg cgtcaggcat gcctccaccc cgcccgcgac
     3481 catgcagtcg gcatcaccgg cctgtatgag cctggccgca acggcaaggg cgtggccgcc
     3541 ggtcgcgcac gcggtcgaca gccccatgcc ggggccctgc gccttcagct tcatcgacac
     3601 ctcggccgag gcgatgttgg ggatgaacat cggcacagtg aacggcgaga ccctcctcgg
     3661 ccccttctcc acgtaaaccg ggatctgctc ctccagcgtg gagatacccc cggccccggt
     3721 accgacgagg acgccgaccc ggtgcgcagg atagtcgttc ctgccgagcc ctgattgcgc
     3781 ccaggcgtcc agggcgcagg tcaccgccat ctgcgcgtag cggtccatgc gcctgatttc
     3841 cttgggaccg agatacttgc cgggatcgaa ccccctggcg cgcccgccga aggtgacggg
     3901 aaggcctgcc agctcgtcta tgggttctat gcctgaaaca ccgttcgtca gattatccca
     3961 gagagttgtg caatcacagc caagtgggga caccacccct atccccgtta ctgccacccg
     4021 tttcttcatc gaggttgatc agatcttcga cttgacgtac tcgatggcgt ccttgacgaa
     4081 cttgatcttc tcggcgtctt cgtcgggcac ctcgatgttg aactcctcct ccacggccat
     4141 gatgagctcg acgaggtcga gtgagtccgc acccagatcg tccgcgaacg acatctccgg
     4201 cttgatctcg gaggggtcct tctccaactg cttggctatc agttgaatga tccgctgctg
     4261 aacatctgcc atcagtacac tcctcctttg aggttctctg catcctcatg ctctaccgtc
     4321 acgtgtagag gccgccgtcc
//
LOCUS       DAOSCR010000175         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12751_length_4336_cov_3.170521, whole genome shotgun sequence.
ACCESSION   DAOSCR010000175 DAOSCR010000000
VERSION     DAOSCR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_4336_cov_3.170521"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLT69_05565"
     CDS             complement(<1..565)
                     /locus_tag="PLT69_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13941.1"
                     /translation="MPGHTTTPGAAAAGPGGIDRRGLIARLTFRLELYINLIVVPLAV
                     YFGTIAGGFEGEKLSCILVSSLVAALLATVFGMSLRIIRLCAVLKGLDAPDADFASTK
                     IRLLSYPAFESAVIALRWVFGMTCVYVMTEYWVGLSFHETLLVFLILVVCIPINSIIS
                     YCTTEHLLAPALMEERIRAVHLPMDSYT"
     gene            1484..2278
                     /locus_tag="PLT69_05570"
     CDS             1484..2278
                     /locus_tag="PLT69_05570"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOJ13942.1"
                     /translation="MSTQGVTAPRIDPRFKPRLGTYYYKFDFNNVSIGFASISIEKDG
                     DLYKVKVLAQTNSTIDRIYKIRYRGESVLDTVPVVSPVVTLSSQQVKSTEKDMTIRFD
                     KPGEIKAVEKKSENGATVDYEVRKLQTDRFTVDPFAATYLVRGLDWKVGHEEVFDVFG
                     GKSRYELRLKCTEVQDVDVEGAKRRAFVIVPRVDKLDKDGKVVPPKKKPAETKIFLSA
                     DEFKDVLKIEASHTMGFFRATMNRFVPLVPQVREHDPAVQAPKEAP"
     gene            complement(2330..2755)
                     /locus_tag="PLT69_05575"
     CDS             complement(2330..2755)
                     /locus_tag="PLT69_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13943.1"
                     /translation="MEEMQQAGFTKEQIEQVISIKVNVREANKKWTEEELRQLDANLR
                     ASWRSMVQALARGDVETASAFFERDMRDIHRMLLAATPHQQRKLLLEALEDIRLLGVK
                     GSNLAEYRVNIPQARTIYTYRGELVFIRNDEGVWEIKSF"
     gene            complement(3576..>4336)
                     /locus_tag="PLT69_05580"
     CDS             complement(3576..>4336)
                     /locus_tag="PLT69_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOJ13944.1"
                     /translation="CLEEHLQRFLSSASQAFMKIPFTAEVIRRAVIETSHANGLKSGI
                     VKCYAYHPDIDVGPATPETTTVAVFCLDFSALGIPHDKYAHPIRLGVSSLRKLHPQTA
                     AVHAKITGNYANGFLARMEARGRGYDEALMLDLDGFVAEAPTANIFLASSGVVVTPPD
                     ECVLPGITRKVVMDVLGDMGVQVRKQPIRVEDLGSFDEGFLSGTLRHVQPLAQIEDCV
                     FACPGPITASLLDRMQEVYEGTAERYRHLLTYLS"
BASE COUNT          736 a         1368 c         1507 g          725 t
ORIGIN      
        1 gggtgtagga gtccataggc aggtgtaccg ccctgatgcg ctcctccatg agcgccggcg
       61 cgaggaggtg ctcggtggtg cagtaagaga tgatgctatt gatcggtatg cacacgacga
      121 ggatcaggaa cacgaggagc gtctcgtgga agctgaggcc cacccagtac tccgtcatga
      181 cgtagacgca cgtcatgccg aacacccacc tgagcgctat cacggcgctc tcgaaggccg
      241 ggtaggacaa cagccgtatc ttggtcgagg cgaaatccgc atccggcgcg tcgaggccct
      301 tgagaacggc gcacaggcgg atgatgcgaa gcgacatccc gaacacggtg gcgagcaggg
      361 cggcgaccag ggaagacacc aggatgcacg agagcttttc gccctcgaac ccccccgcga
      421 tcgtgccgaa gtagacggcg agcggcacca cgatcaggtt gatgtagagc tcgaggcgga
      481 acgtcaggcg tgcgatgagc ccgcggcggt cgatgccgcc cgggcccgcc gcggccgccc
      541 cgggagtcgt cgtgtggcct ggcatggccg gcgtcctcct gcgaacgtga gtgtgtgggg
      601 cgggtcccgc aaggaggcct catccccctg gatctcctct agggggtccg gcggtgcgct
      661 gtcaatgagc gatcccccct cgacaccgaa aaagtgcgcg ctcagacaca gaaaccatca
      721 cctggacgca tgcgtgcgcc gacggaaagt cgttgtcgcg tggcggctcg agtggtcttg
      781 agggtagggt gcctcggggg gtggtcgtac ctgttggcac gcaagatgct tgtctatgtg
      841 tacgatgatg ggtggaggat ctatcacgag gaagaaacgg gagatgcgtg ttcaagggtg
      901 ttgcacgaaa gacacagccg ggtcgtcagg acccggcagg caaccgccgg catcgacggg
      961 tcaggccatg cgcggccggc ttcggccgga tctcgtgggg gaggtgcatc ctttctgcgc
     1021 aaggcctgca gccccacggt acgctgtgtg gggggctgcg gtctcagggc cttgccgcag
     1081 gacatccccc tcgtcgtgcg gggctgccgg ggtcgggacg gccatgcagg cgacgtcggc
     1141 agcggcctcc cgcgggggcc gtgctcgcgg ctgcgggggc tccgcgcagg aggcaccctc
     1201 gtcgggcccc ctcggcgctg ttcgtcgacc cgcctcctgc atcgaggtcg aggacggggg
     1261 tgccgccccc tgggcagggg ttcccggtgg gtttgcgggc ctgcggcgct cacccgcgag
     1321 gacgcgggcc cgggtcccgg cgggtacgtg gacaggtctc gtccgcacag gcctttcttg
     1381 cggaacggcc gcgggaggga tcgcgccgtt tctcctggca accttgctct gctacctcgt
     1441 gatcgcggcc ggaccccagg gggttcaggc ccaggaggcc gtggtgtcaa cgcagggcgt
     1501 gaccgcgccc aggatcgacc cgaggttcaa gcccaggctc ggcacctact actacaagtt
     1561 cgatttcaac aacgtcagca tagggttcgc gagcatctcc atcgagaagg acggcgatct
     1621 ctacaaggtc aaggtgttgg cccagacgaa cagcaccatc gaccgtatct acaagatacg
     1681 gtaccggggg gagtccgtgc tcgacaccgt gcccgtggtc tctcccgtgg tgacgttgtc
     1741 ctcccagcag gtgaaatcga cggagaagga catgaccata aggttcgaca agccggggga
     1801 gatcaaggcg gtcgaaaaga agagcgagaa cggggcgacc gtggactacg aggtgagaaa
     1861 gctccagacg gacaggttca ccgtcgaccc cttcgccgcc acctacctcg tgcgcgggct
     1921 cgactggaag gtgggtcacg aggaggtgtt cgacgtgttc ggcggcaaga gccgctacga
     1981 gctgaggctc aagtgcaccg aggtgcagga cgtggacgtg gagggggcga agcgccgtgc
     2041 gttcgtcatc gtgccgaggg tcgacaagct cgacaaggac gggaaggtcg tcccgcccaa
     2101 gaagaagccc gcggagacca agatcttcct ctccgcggac gagttcaagg acgtgctgaa
     2161 gatcgaggcg agtcacacca tggggttctt cagggccacc atgaaccgtt tcgttccgct
     2221 tgtgccgcag gtccgtgaac acgaccccgc ggtccaggcg ccgaaggagg ccccctgagg
     2281 gggctcgacg ggtgaccggg tgcgtgcccg gggcggggac ttcccgtggt cagaacgact
     2341 tgatctccca gacgccctcg tcgttgcgga tgaagacgag ctctccgcgg taggtgtaga
     2401 tggttctcgc ctgggggatg ttgacccggt actcggcgag gttcgatccc ttgacgccca
     2461 gcagccgtat gtcttcaagg gcctcgagga ggagcttgcg ctgctggtgg ggtgtcgccg
     2521 cgaggagcat cctgtggatg tcccgcatgt ccctctcgaa gaaggccgag gcggtctcca
     2581 cgtcacccct ggccagagcc tggaccatcg atctccagct cgccctgaga ttcgcatcca
     2641 gctgcctgag ttcctcctcg gtccacttct tgttcgcctc ccgcacgttc accttgatgg
     2701 atatgacctg ttctatctgc tccttggtga accccgcctg ctgcatctcc tccacgagga
     2761 ggatctccta gtcggtgagc atggacttgg cccggccctc cttcggccag aggaacgcga
     2821 gcgccgcggc gaggacgatg gcaaccgcct tcatgactcc gcctccttca tggacacacc
     2881 ctctgccggg gacaagcccc gggtctcggg ataatctctt gcgaaaacat attacctgtc
     2941 ggctgcccac tttgcaagtg gatgcacagg gcatgggcga caccccagga ggggtctccg
     3001 gggacggccg tgcagatcct cccgcgcgct cccccgcctc gtcccggccc gcatgaggcc
     3061 catcggggcg cccagctcga cagggaggcg tcttgaggcg cgtgcaatcg tctgtcgccg
     3121 ccatcggccc caggaggcga cgggcgggaa ggggcacggt tgcaggccgt cttcacacaa
     3181 gccggcgttt ccgggtcctc tgtgctcccg cacgcagcgt cgctcatgca gacaacggcg
     3241 cacagagggc atttgtcgtt cccacctctc cgctctcccc gcacgcggcg tcggggatgc
     3301 gggcgacggc gcccgcctca atgcgaggcc gcgtcccggc gagggtggga cccctcccgt
     3361 gagggggatc cccctgcggg atacacgggt atacggtcag gtctgcacct gatggggata
     3421 tccctgcggg atacccgctc catgataagg cccgcgccgc ctcgtgcggc cgccgtgttc
     3481 tccagggggc gccatgcatg cgggagggag cgagacggcc ccgggtgaag acgatcccgc
     3541 agccctgcag gtcgccgtgc aagggatgaa gcccgtcacg agaggtacgt gagcaggtgg
     3601 cggtaccgct cggccgtgcc ctcgtatacc tcctgcatgc gatccaggag cgaggcggtg
     3661 atcggcccgg gacacgcaaa gacgcagtct tcgatctgtg cgagcggctg cacgtgcctg
     3721 agcgtgcccg agaggaaccc ctcgtcgaag gagcccaggt cctcgacccg tatgggctgc
     3781 ttcctcacct gcacacccat atccccgagc acgtccatga ccaccttcct ggtgatgccc
     3841 ggcagcacgc actcgtcggg cggggtcacc acgacgccgg aggaggcgag aaagatgttc
     3901 gccgtcgggg cctcggcgac gaacccgtcg aggtcgagca tgagggcctc gtcgtagccc
     3961 ctgcccctgg cctccatgcg ggccaggaac ccgttcgcgt agttgccggt gatcttcgcg
     4021 tgcaccgcgg ccgtctgcgg gtgcagcttc ctcagggagg agacgccgag gcgtatgggg
     4081 tgcgcgtact tgtcgtgggg gatacccagg gcgctgaagt ccaggcagaa cacggcgacc
     4141 gtcgtcgtct cgggagtggc cgggccgacg tcaatgtccg ggtggtaggc gtagcacttc
     4201 acgatgccgc ttttcaggcc gttcgcgtgc gaggtctcga tgacggcccg ccggatgacc
     4261 tcggcggtga aggggatctt catgaaggcc tgggaggcgc tcgagaggaa ccgctggagg
     4321 tgctcctcca ggcaga
//
LOCUS       DAOSCR010000176         4331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12772_length_4331_cov_3.453695, whole genome shotgun sequence.
ACCESSION   DAOSCR010000176 DAOSCR010000000
VERSION     DAOSCR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12772_length_4331_cov_3.453695"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLT69_05585"
     CDS             complement(<1..758)
                     /locus_tag="PLT69_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOJ13945.1"
                     /translation="MNSNASMMKTIALWVVIALIGVMLFHLFNTPPAKQPKSLSFSEF
                     VQVLEKGGIKEVTIQGNELKGVLSDGKPFETYTPDDPKLVERLMEKNVTIKAKPKDES
                     PWYLSALISWIPMLLLIGVWILFMRQMQTGGTKAMSFGRSKARLMTQDRMKVTFADVA
                     GVDEAKEELEEIIEFLKEPKRFTSLGGRIPKGVLLLGPPGTGKTLLAKAVAGEAGVPF
                     FSMSGSDFVEMFVGVGASRVRDLFAQGKQHAPCI"
     gene            complement(766..1416)
                     /locus_tag="PLT69_05590"
     CDS             complement(766..1416)
                     /locus_tag="PLT69_05590"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ13946.1"
                     /translation="MAIDKKDRLDAPDEFVKSASTTLLWIKEHPLNTAVIALVVLGVV
                     ASGFGLVYWKTSRDTDAMNALLKATDDYDLTLKVTRDYPGTKADKLARLRLARMSYEK
                     GDTAKALPMVEEFIDDWGRQDTFHWQAVLLAAACHRDQKHPDKALALLDSAIGSAGGV
                     LRDHALFMKGSILLSQGKTDEASQALQGVSENYKDMARSALAASRAAAAPAQPPAQ"
     gene            complement(1580..3025)
                     /gene="pilQ"
                     /locus_tag="PLT69_05595"
                     /pseudo
     CDS             complement(1580..3025)
                     /gene="pilQ"
                     /locus_tag="PLT69_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
     gene            complement(3412..3945)
                     /locus_tag="PLT69_05600"
     CDS             complement(3412..3945)
                     /locus_tag="PLT69_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOJ13947.1"
                     /translation="MTRVLTSLFVLCLLMSAASCTGGSQAPAPAPVKKAPAAGKVEAE
                     KPVPVLEQVEEQKPPEYQVVGVRDPFQPFAGLKPAESMISGEIGKGVDPLQQLSLAQL
                     YLVGVIMGKQNKALLQDTTGLGYIVHEGTLIGENNGVVTKITKDSVTVKQHFKDYMGR
                     VSTREVVLALRKEEGVK"
     gene            complement(3942..>4331)
                     /gene="pilO"
                     /locus_tag="PLT69_05605"
     CDS             complement(3942..>4331)
                     /gene="pilO"
                     /locus_tag="PLT69_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOJ13948.1"
                     /translation="VALKQLPDKSEIPTLLENVSSLGTAAGLQFKLFKPMPEVPKNFY
                     AEIPVDIEVEGTFRDVVSFFDNVSKMPRIVNIVNITMDKPHKNPTGGTLVNTTCNAVT
                     YKFIESASPEGGPAKPAGNDTKEVTNK"
BASE COUNT          655 a         1335 c         1388 g          953 t
ORIGIN      
        1 atgatgcacg gcgcgtgctg cttgccctgg gcgaacaggt cgcgcacgcg cgaagccccc
       61 acgcccacga acatctccac gaagtcggaa cccgacatgc tgaagaacgg gacccccgcc
      121 tcgccggcca cggccttcgc gaggagcgtc ttgcccgtgc ccgggggacc gagcaggagg
      181 acccccttcg ggatgcggcc gcccaaggag gtgaagcgct tgggttcctt gaggaactct
      241 atgatctcct cgagctcttc cttggcctcg tcgacgccgg ccacgtccgc gaacgtcacc
      301 ttcatcctgt cctgggtcat gagcctggcc ttgctcctcc cgaagctcat ggccttggtg
      361 ccgcccgtct gcatctggcg catgaacagg atccagacgc cgatgaggag cagcatcggg
      421 atccaggata tgagcgccga gaggtaccac ggcgattcgt ccttgggctt ggccttgatg
      481 gtgacgttct tttccatgag gcgctccacg agcttcgggt cgtcgggtgt gtaggtctcg
      541 aagggcttgc cgtcggacaa gacgcccttg agctcgttgc cctggatggt gacctccttg
      601 atgccgccct tttccagcac ctggacgaac tcgctgaagg acagggactt gggctgcttc
      661 gcaggaggcg tgttgaacaa gtggaagagc atgacgccga tgagggctat caccacccac
      721 agcgcgatgg tcttcatcat gctggcgttc gagttcatcg aatgctcact gggcaggggg
      781 ctgcgccggg gcggctgcgg cccgggaggc ggccagggcc gaccgcgcca tgtctttgta
      841 gttctcggag accccctgga gcgcctggct cgcctcgtcg gtctttccct gggagaggag
      901 gatggaaccc ttcatgaaga gggcatggtc cctcaagacc cctcccgcgg acccgatggc
      961 cgaatccagg agggcgaggg ccttgtccgg gtgcttctgg tccctgtggc acgcggccgc
     1021 gaggaggacc gcctgccagt ggaaggtgtc ctgcctgccc cagtcgtcga tgaactcctc
     1081 caccatcggg agcgccttgg cggtgtcccc cttttcgtag gacatgcggg cgagcctgag
     1141 tcgcgcgagc ttgtccgcct tcgtgccagg atagtccctg gtcaccttga gggtcaggtc
     1201 gtagtcgtcc gtggccttca agagcgcgtt catcgcgtcc gtgtccctgg aggtcttcca
     1261 gtacacgagg ccgaaacctg acgccacgac gcccaggacc accagggcga tgacggcagt
     1321 gttgagggga tgctccttga tccacaaaag ggtggtgctc gcgctcttga cgaactcgtc
     1381 cggcgcgtct agcctgtctt tcttgtcgat tgccatgaaa gcgtcctcca gatcgttagc
     1441 acacatatag tgacacggtg cgttctctgt caatcggtga ttctccccgg atctcctcgc
     1501 ggaggggatg ctgcggaaga aaaggagccc cggcctcgag gccggggctc ctggaatgcg
     1561 tacggtgtcg gtcaggtcgt cactggaacg cgagcggctc tatgatctgc ggcgttatga
     1621 agatgaggag ctccttcttg atcttcgact tttccgtgcc cttgaagaga tagcccagca
     1681 ccgggatgtt ccccaagaac gggacctgcg tgatgttgct ccggtcctct tcctcgatga
     1741 tgccgccgat gacgctcgtc tcgccgttct tcaccaggac ctcggtcacc gcctccctgg
     1801 cggcgatcta cgggtcgccc agctggttcg tgatcgagcc gagggagttg cgtttcacgc
     1861 tcacctccat gttcacgctc ccgtcgaagg cgatgcgcgg ggtgacctcg aggaccaggt
     1921 ctatgtcctt gagctcggtg gagagctggc cgttctcgtc gcggacgatg taggggtatt
     1981 tctcgccctg gcggatggtg gccttcttgt tgtccatggt cacgatcttc ggcctggaca
     2041 gcacgttcac gctgcccaac gtctcgccta tgtccagcct gagatcgagg gtcgctgcgg
     2101 tcgagccgag cctgccgacg gtgatgccga agccggcggc ctggttcgtc gggaagttga
     2161 ccgccatgga gggcgtgggc acgaggtccg tggaccacat gggcctgccg cccgaggtca
     2221 ccgatgcgtc gaacgggttg accgtggcgc ccgtgttcac gccggatagc ccgaacaggt
     2281 acttgccgga ctggttcctc gtcgacccgc cccactggac gccgagtccc ttggtgaagc
     2341 tggtgtccgc ctggacgatc cgcgcggaga tgagcacctg cggggtgggc ttgtcgagct
     2401 tcttcaccat ggccgtgacc tcgtccacgc tcttgcggat atccttgacg atgagcacgt
     2461 tggtcctgtt ctcggcggtg atgttcccgc gctccgagag cagccccacc tccttgccct
     2521 tgatggtcga tgcgatctca gatgccttgg cgaagttcac cgggatcacc gatgtcacga
     2581 ggggctcgag ctgttcctgc gtcttcttcg acgagagctg cctctcctgg gcctgcttga
     2641 tcttgtccgc gggcgcgatg cggatcacgt tgcccatggt catcttgtcg aggtccttgc
     2701 tctcgagcac gacgtcgagg gcctggtccc acgggacgtc cttgagcctg atggtgaccg
     2761 tgcccttgac ctcgtcggag gtcacgatgt tcatgccgct cacgtcggct atgatcctca
     2821 ggatgttgtg gatgtcggcg ttctggaagt cgagcgtgat gcgctcaccc ttgtacttcg
     2881 gccccgtgtc cgtgtcgatg attgtgctct gcggggcctc gttcgtggtg agacccttga
     2941 cgtaactctc ggttttcctg gccgcctgct ggccggacag gaccggcgcc gccttcgggg
     3001 ccggggcggc ggctctcgcc tgcatgtccg ccggggtgtc gaagtccatg atgaggagct
     3061 tgccgtcctg ggacacgaag tagggtgtca cctgcttcaa ggtgatctcg aagacgacgt
     3121 ccgccttgtc ctgtctcggg atgatctggt cgacggcgct tgggaactcc ctcgtgatgt
     3181 aggggcgtgc gaggtgggcc ggtatggtgg ccttgttgat cctcaggatc acgttgttcc
     3241 cggacctgtt cacgtcgtag gatgccttgc cgtcgatacc tatctggagc ctggacgtct
     3301 tgtcgaggtt caggaagtcc acgctcttga tgctcaggcc atgcgcggcc cccggcagga
     3361 ccagtaatcc gatggctagt atggtcaaaa ggatggtgtc tctcttcatg cctactttac
     3421 cccctcttcc ttcctgagcg ccaggacgac ctcgcgggtg ctcacgcgtc ccatgtagtc
     3481 cttgaagtgc tgtttgacgg taacggagtc cttcgtgatc ttcgtgacca cgccgttgtt
     3541 ctctcctatg agggtccctt cgtggacgat gtaccccagt ccggtggtgt cctgcagcag
     3601 cgccttgttc tgtttgccca tgatcacgcc cacgaggtag agctgggcga gggagagctg
     3661 ctgcaacggg tccacccctt tgccgatctc tcccgagatc atggactcgg cgggtttcag
     3721 gcccgcgaag ggttggaacg ggtcacgcac gcccaccacc tggtattccg gcggcttttg
     3781 ctcttccacc tgttcgagca cgggcaccgg cttttccgcc tcgacctttc cggctgcggg
     3841 tgctttcttc acgggggccg gcgccggggc ctgcgacccc cccgtgcagg acgccgccga
     3901 catgaggagg cagagtacga acagtgaggt cagaaccctt gtcatttgtt cgtcacctcc
     3961 ttcgtatcgt ttcctgcggg cttggccggc ccgccctccg gggaagcaga ctcgatgaac
     4021 ttgtacgtca ccgcgttgca ggtggtgttc accagggtcc cgcccgtggg gttcttgtgc
     4081 ggcttgtcca tggtgatgtt cacgatgttc acgatcctcg gcatcttcga cacgttgtcg
     4141 aagaaggaca cgacgtcgcg gaaggtgccc tccacctcga tgtcaaccgg gatctccgcg
     4201 tagaagttct tcgggacctc gggcatgggt ttgaagagct tgaactggag accggcggcg
     4261 gtaccgagcg aggagacgtt ctcgagcagg gtggggatct cgctcttgtc ggggagctgc
     4321 ttcaacgcca c
//
LOCUS       DAOSCR010000177         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12778_length_4330_cov_3.400702, whole genome shotgun sequence.
ACCESSION   DAOSCR010000177 DAOSCR010000000
VERSION     DAOSCR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12778_length_4330_cov_3.400702"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLT69_05610"
     CDS             complement(<1..445)
                     /locus_tag="PLT69_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ13949.1"
                     /translation="MSSESAAYSGCGRSACKASCAACPPTPPAPLSTSHVSGGSFSHA
                     VLTLCVLAMATALAHGCSPGVESPQGARAEIRGVKVSEIRPTTTTSFVEVTGTVRSDR
                     RAAISSKVMGRVSSIAVKEGDLVKTSSLLLTIDDTDIAQRHKAALE"
     gene            complement(620..814)
                     /locus_tag="PLT69_05615"
     CDS             complement(620..814)
                     /locus_tag="PLT69_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13950.1"
                     /translation="MGLALDESKDGDEIFEKGGFTFVIEKKLLEEVKPVTVDYVTTPR
                     GEGFVVKTGIKADCGGCTSC"
     gene            complement(1631..1948)
                     /locus_tag="PLT69_05620"
     CDS             complement(1631..1948)
                     /locus_tag="PLT69_05620"
                     /inference="COORDINATES: protein motif:HMM:NF038090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscA/HesB family protein"
                     /protein_id="HOJ13951.1"
                     /translation="MLEVTRKATEMIGQFFKGREKVEPVRVFVAGMGCSGVQLGMALD
                     EANENDRTFEIDGYTYLIENQLYETAKPIKIDYVTTSQGEGFVISSSLKPGSGCSGGC
                     CGC"
     gene            complement(2459..2695)
                     /locus_tag="PLT69_05625"
     CDS             complement(2459..2695)
                     /locus_tag="PLT69_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOJ13952.1"
                     /translation="MARRGRKPLSSKYEGTDTKLVKRNYYIEERHAKALSILSAITGK
                     ELSILVNEAIGMLIKKYQPKTDINLLDLKEVIKA"
     gene            3384..>4330
                     /locus_tag="PLT69_05630"
     CDS             3384..>4330
                     /locus_tag="PLT69_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13953.1"
                     /translation="MGFERIWHKSYPQGVPREIVFDRTTMPEALERSSRRFPDTPALN
                     YMGRLITYEELNRMVNAFARALMGLGVKKGDTVAMLLPNIPQVVIANYATWRIGAVAA
                     QNNPLYTERELTHQLDDSDATILVTLDLLLPRALAVKPKTKIRHIVICHINDYLPFPK
                     KQLFPLVKRKMYRKVKPGDGVSLFVDLVEANDDSPVPNHAAWEELGTLIYTGGTTGVS
                     KGVMLTHANMSCNVQQLRAWFYDIEDGKESILGIFPFFHSAGFTGIQNFTVWGGGTDV
                     LVPRPEPGVIIELLKKFRPGFMPGVPTIFIGLLNDERFR"
BASE COUNT          719 a         1406 c         1326 g          879 t
ORIGIN      
        1 cctcgagggc ggccttgtgc ctctgggcga tgtccgtgtc atctatggtg aggagaagcg
       61 aagaggtctt caccaggtcc ccttccttca cggcgatgga gctcaccctg cccatgacct
      121 tgctcgagat ggccgccctc ctgtccgagc ggaccgttcc cgtgacctcg acgaagctcg
      181 tagtcgtcgt gggcctgatc tcggacacct tcacccccct gatctcggcc ctggcccctt
      241 gaggcgactc cacgcccggt gagcagccgt gagcaagggc cgtcgccatg gccaggacgc
      301 agagcgtgag gaccgcgtgc gagaacgatc caccgcttac gtgcgaggtg gacaggggcg
      361 caggtggagt ggggggacat gccgcgcagc tcgccttgca ggccgacctt ccacagccgc
      421 tgtaggcggc ggattccgag gacatggacg gcactgccct cacctccaaa gagatatttc
      481 acgccggcag tggactcgat ccacccggca gactcgttga tagtatagcc gcggtatacc
      541 ggtgagacaa gccctccacc cgccatggcg gcgggtggag ggctcttggg tcaggtggtg
      601 gtgcggccgg ggtcgcgtct cagcagctcg tgcacccccc gcagtccgcc ttgatgcccg
      661 tcttcaccac gaacccctcg ccgcgcgggg ttgtgacgta gtccacggtc accggcttca
      721 cctcctcgag gagcttcttc tcgatgacga aggtgaatcc gcccttttcg aagatctcgt
      781 ctccgtcttt tgactcgtcc agagccaggc ccatctgggg cccgcctcag cccacgcccg
      841 cgatgaagac cctcaggggc tcgacgccct ggcggctctt gaagaactcg cctaccatct
      901 cgatcgcctt ttccgttacg gtgaacatcg tgttcctcct tttttcgtgg agtgagcccc
      961 aggccgagga ggtccttggc gcaccccggg tttatatcct ttcagccgtt cttgttgtat
     1021 cgtccggccg tcttctccag tcaacctcgt gcacccgtgc tcgtgcccga ggacaccgga
     1081 cgggttcccc cctcttaccc gatctttcct tccccatcaa ggccgctggg acggtgctgt
     1141 cgaccggcac gcccggcggg ccggacctgt ttgtacaacc ggtttgtaca gatggatccc
     1201 cgtgcttagc catggaagcc ttgctgtccg ggtgccttct cccgggcggg cggatgctcc
     1261 cactgccttg ggatgagccg gcaggggatg tccccaaggt ctttctgagg gggatgcccc
     1321 ccctgccttg ggggtgaacc gccgtcccgg cacgagaacg aggccgaccg tgaaggtctt
     1381 gctccatgat cccccacgcc ctggcaggca aatcgcgaga cgctatggga ccgaacccgg
     1441 cctggacggc ggtaccgtcg tccagggcac gatgaggggc ggcctgctct tgcctggtat
     1501 cacggccagg acgctcgcct gccgagagac ggaaggcgcc ggtccgccgg cgcctgcccc
     1561 cggtgcctcg aggcctcggc agaccatgca tgacctcatg tgtcacatcg gtttgcacgt
     1621 tgtcgtgcgc tcagcaccca caacaccccc ccgagcaccc ggagcccggc ttgagggaag
     1681 aggagatgac gaagccctct ccctgcgagg tggtcacgta gtctatcttg atgggcttgg
     1741 ccgtctcgta gagctggttt tcgatcaggt aggtatagcc gtcgatctcg aacgtgcggt
     1801 cgttctcgtt cgcttcgtca agggccatgc ccagctgcac gccgctgcac cccatgccag
     1861 ccacgaatac ccgtacgggc tccacctttt cccttccctt gaagaactgc ccgatcatct
     1921 ccgtcgcctt cctggtcact tccaacatct tctgtctcct tgtcgggcgg ttgcatgggc
     1981 tttctgtcgt tttgagccag acaaccagca gtcacccttt cgttgtatgc gccttctcaa
     2041 ggtttcgcgc cccgcggccg cgacccggcg caatggtccg gccgtgtggt cccctcgtcg
     2101 agcgggatcc tttagcgtcc gtgttcatcg tctgtcttca ggtccccatg cgtccgtcgg
     2161 tgcaccctgc cacatgggga tgcttcggtc aacccgtatc gggggtgtcg atggggggtg
     2221 tctctctcgt ccttggccct tgacgccgca caggagcccg ttggctggcg cgagcgtgat
     2281 cctcaaggtt cgcggcgctc tgctccggcg attcatcact ccgaggcggc cctctccctc
     2341 aagccaaggc ctgatctcgg ccgggtcacc caccatgccc gaagggccgt gtctgtgcgg
     2401 ccggtggtgc atgtggccgg gcgtggcgca ggggtcgaaa ggtcgggccc gagaccggct
     2461 aggccttgat gacctccttg aggtcgagga ggttgatgtc cgtcttgggc tggtacttct
     2521 ttatcagcat cccgatcgcc tcgttcacca ggatggagag ctccttgccc gtgatggcgg
     2581 acaggatcga gagggccttt gcatggcgtt cctcgatgta gtagttccgc ttgacgagtt
     2641 tcgtgtccgt gccctcgtac ttgctgctca atggttttct tccgcgtctt gccatgtgtc
     2701 tgtcctttcc ctctcgtgca acatgatagt ctatattatt tagaacatcg aagatcgaca
     2761 aggattctcg tatcacgaaa tatgtacgct ccccgccatg cgccgacccc ctgtgtccgc
     2821 gatttcactg gctggttttt cgagagacca gggtaactcc cctcttcatg acgacggctc
     2881 gcaggatgaa gacccgaggg cccgccttcc ggaaagaacc gggtgagtgc acgaggagac
     2941 ctcgtttgcg tggcgggaaa cgagacgtca ctccttcccg gcgcagcgcc tggttgcatc
     3001 ccgatgggac acgagccccc tcatccttgg gcggtcgcac ggacagccat gaaaggcgtg
     3061 tgctcagtgt ttcggaaggc gcctcttcgc gcatgggagg catacccttt tgcgcaggga
     3121 agagacccac gggtacccga gaacatggaa ggggcccgcc ggcggaccct ttgcgcaggg
     3181 agagggcgcg ggggtcctcc ctcaccgaac gaccgtttcc agcgtggccg gtgtgccatg
     3241 gccaaggtga ggcgggtcct cccgtgcatc tcgggtcaca ggtcgcggta gggtcttgac
     3301 agggcggggc atcgtgttag tattgtatga taataagtca acttggatgc atgacccgaa
     3361 tgcttcggat gggggaggtt cgcatgggat tcgagaggat ctggcacaag agttatccac
     3421 agggggtccc cagggagatc gtcttcgacc gcaccacgat gcctgaagcg ctcgagagga
     3481 gctcgaggcg ttttcccgac acgccggcgc tcaactacat gggcaggctg atcacgtacg
     3541 aggagctcaa ccgcatggtg aatgccttcg ccagggccct catggggctc ggggtgaaga
     3601 agggcgacac ggtcgccatg ctcctgccca acatccccca ggtggtcatc gccaactacg
     3661 cgacgtggcg gataggcgcg gtcgccgcac agaacaaccc gctctacacg gagcgggagc
     3721 tcacgcacca gctggacgac tccgacgcaa cgatcctcgt caccctcgac ctccttctgc
     3781 cgagggctct cgccgtcaag ccgaagacaa agatcaggca catcgtgatc tgccatatca
     3841 acgactacct gccgttcccc aagaagcagc tcttccccct ggtgaagagg aagatgtacc
     3901 gcaaggtgaa gcccggtgac ggggtctcgc tctttgtgga cctcgtcgag gcgaacgacg
     3961 actccccggt tcccaaccat gccgcgtggg aggaactggg cacgctcatc tacaccggcg
     4021 ggacgacggg cgtgagcaag ggcgtcatgc tgacgcacgc caacatgtcg tgcaacgtcc
     4081 agcagctcag ggcgtggttc tacgacatcg aggacggcaa ggagagcatc ctcgggatct
     4141 tccccttctt ccactcggcc gggttcacgg gtatccagaa tttcaccgtg tggggcggcg
     4201 gcaccgacgt tctcgtgccc aggcccgagc ccggggtgat catcgagctc ctcaagaagt
     4261 tcaggcccgg cttcatgccc ggcgtgccca cgatcttcat cggcctgctc aacgacgagc
     4321 gtttcaggaa
//
LOCUS       DAOSCR010000178         4326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12791_length_4326_cov_3.288457, whole genome shotgun sequence.
ACCESSION   DAOSCR010000178 DAOSCR010000000
VERSION     DAOSCR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12791_length_4326_cov_3.288457"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..2901
                     /locus_tag="PLT69_05635"
     CDS             265..2901
                     /locus_tag="PLT69_05635"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ13954.1"
                     /translation="MLDREYDPKDIEQKWYDVWLEKGYFHADELDDTRPPYSIVIPPP
                     NVTGVLHMGHALNNTLQDVMIRYKRMDGYNALWMPGTDHAGIATQNVVEKALAERGLS
                     RHDLGRDAFVEEVWKWREKHGGLIIHQLKRLGASCDWERTRFTMDEGLSHAVRKVFVA
                     LYRDGLIYRSDYIVNWCPRCHSALSDLEVEHEEEDGHLWHIRYPLADGDGGIVVATTR
                     PETMLGDTAVAVNPSDERYRDMIGRQLILPVMNRRIPVIADAVVDKEFGTGAVKITPA
                     HDLNDYEAGLRHGLEIVVVIDDSGVMNANAGPYEGLDRFECRRRLELDLEAQGYLVDK
                     TPYRVPVGKCYRCKTTIEPYLSKQWFVRTKPLAEEAIRAVHDGRTRIVPGNWEKVYYE
                     WMNNIRDWCISRQIWWGHRIPAWYCDGCGAVIVDEVEPKTCGACGNAELRQETDVLDT
                     WFSSGLWPFSTMGWPEDTPTLRRFYPTDVLITGFDILFFWVARMMMLGLRFMGEVPFR
                     DVYLHALVRDEQGQKMSKSKGNIVDPILVMDTYGADAFRFALCAFAAMGRDVRISDKR
                     VQGYRYFVNKIWNAGKYVLSNLAGFDPARPEPGPDDLGLAEKWILTRLDEAVEQTRSA
                     LDEYRFNDAAETLYHFAWHTFCDWYIEFSKSTVNTESGHATKWVLHHVLKTLLELLHP
                     IIPFVTEEIWQSLPGKGGESIVIAAYPKQKAGFRFLAEAGLMDTVIEVISGVRSIRGE
                     TGIAPSVLLDVVVKTDDPAMRRLVEEHSVYIANLARARRVACIADESERPKKCALAVR
                     KGLEVYVPLEGVVDIEKELERLSKQMKKVEKELGEKRRKLDNPTFLEKAKKEVVEEQE
                     RLARELSFELASLQKAFDMLAG"
     gene            2910..3608
                     /locus_tag="PLT69_05640"
     CDS             2910..3608
                     /locus_tag="PLT69_05640"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOJ13955.1"
                     /translation="MDKTGIFTEVVKLEEVDSTNRYALETGRSGVIVAALGQSAGRGR
                     RGRSWFSPPGENLYITYTLSPVREHYPIVAGVAARQALSGLVPGVEVLLKWPNDVVAA
                     GRKLCGILCESSYGITAVGMGVNVNQKAWPDPLAPRAVSLSQLAGRDFDLDEVASRVT
                     DSLSEWVERYGREGFEPVRVEFLVHGMLKGLEVRDDTGEPCTIVDLTLDGHLVVETRT
                     GRRELVSEGISIGW"
BASE COUNT          796 a         1339 c         1492 g          699 t
ORIGIN      
        1 catcgtggta gaccctgttc aggcatgtct cgggttctcg acaggggtag cgtctaagcg
       61 tcccgggccc gagggttcga cccggtacgc ccatatcggc gaggtctgtg cctctgcacg
      121 ctcagggcat caagcggcgc ctcgagcgtg agcggtcttc ggtgcaggga tcggtcaggg
      181 acctgcccga cggcatcgcc gctttgccac cacagccctg tttatgctat gtggacacca
      241 ccacattgct cgcaggagtg cgacatgctc gacagagagt acgacccgaa ggacatcgaa
      301 cagaagtggt acgacgtgtg gctcgagaag ggctacttcc acgccgacga gctcgacgac
      361 accaggcccc cctattcgat cgtgatcccg ccgccgaacg tgaccggcgt gctccacatg
      421 gggcacgccc tgaacaacac cctgcaggac gtcatgatcc gttacaagcg catggacggc
      481 tacaacgccc tgtggatgcc cggcaccgac catgccggca tcgcgaccca gaacgtggtg
      541 gaaaaggcgc tcgccgaacg gggcctttcc aggcacgacc tggggaggga tgcgttcgtc
      601 gaggaggtct ggaagtggag ggagaagcac ggcggcctca tcatccacca gctcaagcgc
      661 ctgggtgcgt cgtgcgactg ggagaggacc cggttcacca tggacgaggg gctctcgcac
      721 gccgtgcgca aggtcttcgt ggcgctctac agggatgggc tcatctacag gagcgactac
      781 atagtgaact ggtgccccag gtgccacagc gccctgtcgg acctcgaggt ggagcacgag
      841 gaagaggatg gtcacctctg gcacatccgc taccccttgg cggacgggga cggggggatc
      901 gtggtggcga ccacgaggcc cgagaccatg ctcggagaca cggcggtggc cgtcaacccc
      961 tcggacgagc gctaccgtga catgatcggc aggcaactca tcctcccggt gatgaaccgc
     1021 aggatccccg tcatcgccga cgcggtcgtg gacaaggagt tcgggaccgg ggcggtgaag
     1081 atcaccccgg cgcacgatct caacgactac gaggccggcc ttaggcacgg cctcgagatt
     1141 gtcgtggtga tagacgactc cggggtcatg aacgcaaacg ccggccccta cgaggggctc
     1201 gaccggttcg agtgcaggag gcgtctcgag ctcgatctcg aggcccaggg atacctcgtg
     1261 gacaagacgc cgtaccgggt gcctgtgggc aagtgctatc ggtgcaagac cacgatcgag
     1321 ccctacctct ccaagcagtg gttcgtccgc accaagccac tcgccgagga ggcgatcagg
     1381 gcggtacacg acggcaggac gaggatcgtc cccgggaact gggagaaggt ctactacgaa
     1441 tggatgaaca acatccgcga ctggtgcata tcgcgccaga tctggtgggg ccacaggatc
     1501 ccggcctggt actgcgacgg ctgcggcgcg gtgatcgtgg acgaggtgga gccgaagacg
     1561 tgcggcgcct gcgggaacgc cgaactcagg caggagaccg atgtcctgga cacctggttc
     1621 tcctccggcc tctggccgtt ctccaccatg gggtggcccg aagacacgcc gacgctcagg
     1681 cgtttctacc ccacggacgt cctcatcacg ggcttcgaca tcctgttctt ctgggtggca
     1741 cgcatgatga tgctcggcct caggttcatg ggggaggtcc ccttcaggga cgtgtacctg
     1801 cacgccctgg tccgcgacga gcagggccag aagatgagca agtcgaaggg gaacatcgtc
     1861 gacccgatcc tcgtgatgga cacctacggt gcggatgcct tccgcttcgc gctgtgcgcg
     1921 ttcgcggcca tgggcaggga cgtgcgcatc agtgacaaga gggtccaggg ctaccgctat
     1981 ttcgtcaaca agatctggaa cgcgggcaag tacgtgctct cgaacctcgc cgggttcgac
     2041 cccgcgaggc ccgagccggg cccggacgac ctggggctgg ccgagaagtg gatactcacc
     2101 aggctcgacg aggcggtgga acagacccgg tcggcgctcg acgagtaccg gttcaacgat
     2161 gccgcggaga cgctctatca cttcgcctgg cacaccttct gcgactggta catcgagttc
     2221 agcaagtcca cggtcaacac cgagtcgggc catgcgacga agtgggttct ccaccacgtc
     2281 ctcaagaccc tgctggagct cctccacccc atcatcccgt tcgtgactga ggagatctgg
     2341 cagagcctgc cgggcaaagg cggggagtcg atcgtgatcg ccgcctaccc gaagcagaag
     2401 gccggcttca ggttcctcgc tgaagcgggg ctcatggaca cggtcatcga ggtcataagc
     2461 ggggtgcgca gcatcagggg cgagacgggg atcgcaccct cggtcttgct cgacgtggtc
     2521 gtcaagacgg acgacccggc catgaggcgt ctcgtcgagg agcattcggt ctacatcgcg
     2581 aacctcgcgc gcgccaggcg cgtcgcatgc atcgccgacg agtccgaaag gcctaagaag
     2641 tgcgcccttg cggtcaggaa ggggctcgag gtgtacgtgc cgctcgaggg ggtggtcgac
     2701 atagagaagg agctcgagcg cctctccaag cagatgaaga aggtggaaaa ggaactcggg
     2761 gagaaacgcc gcaagctcga taaccccacc ttcctggaga aggcgaagaa ggaggtcgtg
     2821 gaggagcagg agcggctggc cagggagctc tccttcgagc tcgccagcct gcagaaggcc
     2881 ttcgacatgc tggccgggtg atcgtcggcg tggacaagac cggcatcttc accgaggtcg
     2941 tcaagctcga agaggtggac tccacgaacc gctacgcgct cgagacgggc cggtccggtg
     3001 tgatcgtcgc ggcgctcggc cagagcgccg gcaggggcag gcgggggagg tcgtggttct
     3061 ctccgcccgg ggagaacctc tacatcacct acaccctctc gccggtgcgc gaacactacc
     3121 ctatcgtggc gggggtggcg gccaggcagg cgctctccgg gctggtcccg ggcgtcgagg
     3181 tcctcctgaa gtggccgaac gacgtggtgg ccgcaggcag gaaactctgc ggcatcctct
     3241 gcgagagctc ctacgggata acggcggtgg gcatgggcgt caacgtgaac cagaaggcgt
     3301 ggcccgatcc gctcgctccc agggcggtgt cgctgagcca gctcgccggg cgggacttcg
     3361 acctggacga ggtcgcgtcc cgcgtgaccg acagcctctc cgaatgggtg gagaggtacg
     3421 ggagggaggg gttcgaaccc gtcagggtgg agttcctcgt gcacggcatg ctgaaaggcc
     3481 tcgaggtgag ggacgacacc ggcgagccgt gcacgatagt ggacctcacc ctcgacggcc
     3541 atctcgttgt cgagacgaga acgggcaggc gagagctcgt gtccgaaggt atctccatcg
     3601 ggtggtaggc ggttcatgcc cggacccgcc ctggggcggg ggcccagaga gggcatcctt
     3661 gcccgccgta tcgaggccgc cgcacccggc cttgcgtcgc tcggacaggg tgtcacccgg
     3721 ccaggccggc cggtgtcccg aacgaccaat ggcaggtcct cgggtgggaa gaccgcgagg
     3781 ctctgccggg tttccggccc atcccctctg cccggccctc gagacgggca aacgctcgtt
     3841 tctcgtgcac aggtgggcac gatgtctcgg tcagccgtag atcctccttg ccgatggtcg
     3901 tttctcgtgc acgggtgggc tcgataccag gatctcgcgg cgacgtcgac cgcggtaccg
     3961 gccttccctc gtgggtcgcg gccccgagtg caagaagggg tctccccggt aacgcccccg
     4021 aagggcggcc ccgggcccgt gtcgccccaa gcgtttctcc ccgtccgtgg tgtttccggc
     4081 cgcgttgggc cccagggtga ggatgtcccc cggcgcccgg tggccgcaag ggtttgggcc
     4141 ccaaccaggg tcttgccttt cgtcgagaag cgactacctt aagtggaggg agggaaggat
     4201 catgcgaatg gacaggaata tcaaggaaag aggccacgat ccgtacgcag aggcacggaa
     4261 gtacccggag ggcgcctatt gtcccgagtg tggtgccgtg tacaggcagg ggcggtggat
     4321 ttggtc
//
LOCUS       DAOSCR010000179         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12866_length_4308_cov_3.076417, whole genome shotgun sequence.
ACCESSION   DAOSCR010000179 DAOSCR010000000
VERSION     DAOSCR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12866_length_4308_cov_3.076417"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLT69_05645"
     CDS             complement(<1..313)
                     /locus_tag="PLT69_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13956.1"
                     /translation="MGTVFKKELTPMKELARAFSAKELAPNIREHDRHPFAPLFMDTL
                     EKACEAGFFGVVIPEELGGAGGDLETLCVLLEEISSVDASLGAAVFTNACAQLTAGAC
                     AG"
     gene            complement(330..1397)
                     /locus_tag="PLT69_05650"
     CDS             complement(330..1397)
                     /locus_tag="PLT69_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOJ13957.1"
                     /translation="MAEGERSGWPCLISLAVTEPGAGTDVEEVELLDKGSVSCHARKV
                     KGGYVVNGTKVFISMGHLSTWTVLYAYTDLRHPAESTVGLMVKTGMKGFSLGSHEDKM
                     GQRACPASELVFEDCFVPDELVLFDEGHARAFSKKPRRTLAQTYIDYVVSATRPGVCA
                     FGVGAARGALEKALDYASKTQLNGRPLIDHEWVQSRLAQMYANVALGRLAYQEANHAN
                     SLRGLYRLLQFKPLYYYFLLMPDAYFKRVISPTLDKRITTNLMGRVSFDWAKQEDIRC
                     CSGWASLAKITGTDLGLANCRAALEIMGAAGLRHDLGAEKLLRDIKLLQIYEGTNQLN
                     RLNLFKCLIAPSFPKARMFDE"
     gene            complement(1361..1834)
                     /locus_tag="PLT69_05655"
     CDS             complement(1361..1834)
                     /locus_tag="PLT69_05655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13958.1"
                     /translation="MVSRAEIRARLSNFPPFDDASVGLSYGVRMPRHMVKQIREVVSI
                     ARAFNEQVARPLSLEIDRKTQEDPSYMPRELVETANRWGFYTMWIPRMFGGKGYNLPS
                     MSYFSEEVASACLGIMNVIGVHYLGVAGVISLRTRAWPSVSSRRWRRESARGGRA"
     gene            complement(1905..2588)
                     /locus_tag="PLT69_05660"
     CDS             complement(1905..2588)
                     /locus_tag="PLT69_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ13959.1"
                     /translation="MPSFEDRKAETREMIIRAGCKVFSEKGYHKAQVADIVREAGIST
                     GSIYAHFRDKRDLYEQILRENLESLRNKLKELSQTKTPGDVRERVSMWRPAFTAFFDY
                     VEEHPDQILLMVRGGLGMDYDEDLAWEFFDAFATDIADDFRKWEDLGFISGVNAPLMG
                     HIITGMCLHVALSYLKTRRFSRTEAIETLMAATYAMVSWYLTEKGKKELSGMMADQAC
                     RAGLPEPPL"
     gene            complement(2996..>4308)
                     /locus_tag="PLT69_05665"
     CDS             complement(2996..>4308)
                     /locus_tag="PLT69_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ13960.1"
                     /translation="TIAGRHVYKDIMEVPGKVDLAVVTIPARGVMDLLPRLAAKGIRS
                     VLLISSGFAETGDAGRRLQEELVEAAKRLGLVILGPNTMGIANPHINLYCTGSPVRPR
                     AGGTAVVAQSGNMGVQLLGFAEEQGIGIRGFCGSGNEAMITVEDFIEGFALDETTTIV
                     MLYIEGVRDGGRFFESARKLSRKKPIVLLKGGQSEAGNRAASSHTGAMTSDARVFDAV
                     CRQAGIVKVEQSMDLLDLAAAFSSLPLPGGNRVAVMTLGGGWGVITADLCAQYGLEVP
                     ALSPRLVEKLDEILPPFWSRSNPVDLVGEPSNSIMTTVMEELMQWDGCDAIINLGIMG
                     RALYFRKMADAVRRADPSYTQEFLENAVRYMHTFEEDYLRLIARLMDTYGKPVFGVSL
                     MTDEKSKTVYTVEGFTSKPVFYQTPERAVKACARMYEYKRFRES"
BASE COUNT          721 a         1414 c         1342 g          831 t
ORIGIN      
        1 ctccggcgca ggcgcctgcc gtgagctggg cgcaggcgtt ggtgaacacg gccgccccca
       61 gagacgcatc cacgctcgag atctcctcga ggaggacgca cagcgtctcg aggtccccgc
      121 ctgccccgcc cagctcctcg gggatgacga ccccgaagaa ccccgcctcg caggccttct
      181 cgagggtgtc catgaacaac ggcgcgaacg ggtgacggtc gtgctcgcgg atgttcgggg
      241 cgagctcctt ggcggagaac gccctcgcca gttccttcat gggtgtgagt tccttcttga
      301 atacggtccc catgaaacct cccatacgtt cactcgtcga acatgcgcgc tttggggaag
      361 gatggcgcga tgaggcactt gaagaggttg agcctgttga gctggttggt cccctcgtag
      421 atctggagga gcttgatgtc gcggagcagc ttctcggccc ccaggtcgtg cctgagcccg
      481 gcggcgccca tgatctccag ggccgcgcgg cagttggcca ggcccaggtc cgtccccgtg
      541 atcttggcga gagaggccca accggaacag cacctgatgt cttcctgctt cgcccagtcg
      601 aacgataccc tgcccatgag gttcgtggtg atcctcttgt cgagggtggg agatatcacc
      661 ctcttgaagt aggcgtccgg catgaggagg aagtagtagt agaggggctt gaactggagc
      721 agcctgtaga ggcccctgag gctgttcgcg tggttggcct cctggtaggc gagccttcca
      781 agggcgacgt tggcgtacat ctgggcgagc ctgctctgca cccactcgtg atcgatgagc
      841 ggcctcccgt tcagctgcgt cttcgatgcg tagtcgagcg ccttctcgag ggcgcccctg
      901 gccgccccca caccgaaggc gcagacgccc ggcctggtcg cggagacgac gtaatcgatg
      961 taggtctgtg ccagggtgcg cctcggcttc ttggagaacg cccgagcgtg gccctcgtcg
     1021 aacaggacca gctcgtccgg gacgaagcag tcctcgaaca cgagctcgct cgcagggcat
     1081 gccctctggc ccatcttgtc ctcgtgggaa cccagggaga accccttcat gccggtcttc
     1141 accatgaggc ccacggtcga ttcggccggg tggcggaggt ccgtgtaggc gtacaggacg
     1201 gtccatgtcg agaggtgacc catggagatg aacaccttgg tgccgttgac cacgtaacca
     1261 cccttgacct tgcgcgcgtg gcaggagacg ctgcccttgt cgaggagctc cacctcctcc
     1321 acgtcggtgc ccgcgccggg ctcggtcacc gcgagcgaga tcaggcacgg ccaccccgag
     1381 cgctctccct ccgccacctc cgagaggaca cgcttggcca ggcgcgtgtt cgcagcgata
     1441 tgacgccggc gacgcccagg taatggacgc cgatcacgtt catgatgccc aggcaggctg
     1501 acgcgacctc ttcggagaag taggacatgg acgggaggtt gtagcccttg ccgccgaaca
     1561 tcctcggtat ccacatggtg tagaagcccc acctgttggc ggtctccacc agttcgcgcg
     1621 gcatgtacga ggggtcttcc tgcgtcttcc ggtcgatctc gagggagaga ggcctggcga
     1681 cctgttcgtt gaacgcgcgc gcaatggaga cgacctcgcg gatctgcttc accatgtggc
     1741 ggggcatgcg caccccgtag gaaagcccca cgctcgcgtc gtcgaacggg ggaaagttcg
     1801 acagccgcgc cctgatttca gccctggata ccatgttcac ctccttgcaa agctgttggg
     1861 aaaggcgctc gtgcagcgtc ttgtatggac gctgcgcccc gaactcacag gggtggttca
     1921 gggagacccg cccggcatgc ctgatcggcc atcatgcccg aaagctcctt tttgcctttt
     1981 tccgtgaggt accacgagac catggcatac gtcgccgcca tgagggtctc gatggcctcg
     2041 gtacgcgaaa accgcctggt cttcaggtag ctcaaggcca cgtggaggca catgccggtt
     2101 atgatgtgcc ccatgagggg tgcgttgacc ccggaaatga agcccaggtc ctcccacttg
     2161 cggaagtcgt ccgcgatgtc cgtggcaaag gcgtcgaaga actcccacgc gagatcctcg
     2221 tcgtaatcca tgccgagccc ccccctcacc atgaggagga tctggtccgg gtgttcttcc
     2281 acgtagtcga agaacgccgt gaacgcgggc ctccacatgc tcacgcgctc gcggacgtca
     2341 ccgggcgtct tcgtttggct cagttccttg agcttgttcc tgagcgactc gaggttctcc
     2401 ctgaggatct gctcgtagag gtcgcgcttg tccctgaagt gggcgtagat gctcccggta
     2461 gagatgcctg cctcgcgaac gatgtccgct acctgggcct tgtggtaccc cttctcgctg
     2521 aacaccttgc acccggccct gatgatcatc tcgcgggttt cggctttgcg gtcttcgaac
     2581 gatggcacga ggcctcctgc aaaaatatat cgtaaaaaaa tagaatcaac gtttctaaac
     2641 tataatactc ctcgttcgat tgtcaagtcg aaccaggcat gagacaatgc gggatacacc
     2701 cggggcatgg attcccgctc ggagcgctcc tcgtcgagac gacaggcgac ctggtctaga
     2761 agcacccagg gttggactcc cacgccggcc tcttcgcggc cgacgaccac ccgtcaatcg
     2821 attcctgacg gccgcggggg tggaggcagg accgcctccg gccccgcttc cccgtccatc
     2881 gtcccctggc ggccgcgggg gtggagtgcg aatccctggg tgcctgcatg ccgggcttcg
     2941 cctcacggga tagaggccgc tcgagaggcc gggcagtgcg ttttttttgg gtccctcagg
     3001 attccctgaa ccgcttgtac tcgtacatgc gcgcgcaggc cttcacggcg cgttcgggtg
     3061 tctggtagaa cacgggcttg ctcgtgaacc cctccaccgt gtagacggtc ttgctctttt
     3121 cgtcggtcat gaggctcacg ccgaacaccg gctttccgta cgtgtccatg agcctcgcaa
     3181 tgagcctcag gtagtcctcc tcgaaggtgt gcatgtacct gaccgcgttc tccaggaact
     3241 cctgcgtgta ggaagggtcg gcccgcctca ccgcgtcggc catcttcctg aagtacaggg
     3301 ccctgcccat gatgccgagg ttgatgatgg cgtcgcatcc gtcccactgc atgagctcct
     3361 ccatgaccgt cgtcatgatg ctgttgctcg gctcgcccac gaggtccaca gggttggacc
     3421 tgctccagaa gggggggagg atctcgtcga gcttctccac gagccgggga gaaagcgccg
     3481 ggacctcgag cccgtactgg gcgcacaggt cggccgtgat cacgccccac ccaccgccca
     3541 gggtcatgac cgcgaccctg ttgccgccgg gcaggggaag ggaggagaac gcggccgcca
     3601 ggtccaacag gtccatggac tgctcgacct tcacgatgcc tgcctgcctg cacaccgcgt
     3661 cgaacaccct ggcgtcagac gtcatggcgc cggtgtggct ggatgccgcc ctgttgccgg
     3721 cctcgctctg gccccccttg aggaggacta tgggcttctt cctggacagc ttcctcgcgc
     3781 tctcgaagaa ccggcccccg tcgcggacgc cctcgatgta gagcatgacg atcgtggtgg
     3841 tctcgtcgag ggcgaacccc tcgatgaaat cctccaccgt tatcatggcc tcgttgcccg
     3901 agccgcagaa ccccctgata ccgatcccct gctcctcggc gaaccccaag agttgcaccc
     3961 ccatgttgcc cgactgcgcc acgaccgctg tcccgcccgc cctcggccgc accggggacc
     4021 ccgtgcagta gaggttgatg tgggggttcg cgatccccat ggtgttgggc ccgagaatga
     4081 cgaggccgag cctcttggcc gcctccacga gctcttcctg gagccgcctg ccggcatccc
     4141 ctgtctcggc gaaaccggac gagatgagca gtacgctccg gatgcccttt gcggccagcc
     4201 gcggcaggag gtccatgaca cccctggcgg ggatggtgac gaccgcgagg tcaaccttgc
     4261 cgggcacttc catgatgtct ttgtatacgt gcctgcccgc gatcgtgc
//
LOCUS       DAOSCR010000180         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12910_length_4297_cov_3.225365, whole genome shotgun sequence.
ACCESSION   DAOSCR010000180 DAOSCR010000000
VERSION     DAOSCR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12910_length_4297_cov_3.225365"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLT69_05670"
     CDS             <1..782
                     /locus_tag="PLT69_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOJ13961.1"
                     /translation="GDTMFVVPSEKKAKEIVEYLQNKEMVSRLQAPEQKPQVTLEDLY
                     SQVQEGKIKDLNLIVKGDVHGTVEAIVSSVKGIDTSGHDIRINVIHSAVGGITENDVL
                     LASTSSAIIVGFNVRPETKARDLAKKHKVEIKLYTVIYDLIEDIKQALRGMLEPVYRE
                     VVLGRAEVREIFKIPKVGVVAGCMVTEGTIQRTSSVRLLRDNVIVHEGKVDSLRRFKD
                     DVREVQMGYECGIGLGGYNDIKVGDVIEAFTMEKVETTSSW"
     gene            776..1060
                     /locus_tag="PLT69_05675"
     CDS             776..1060
                     /locus_tag="PLT69_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOJ13962.1"
                     /translation="MVIAALEVRLRIPGAHSLKEKRRVIKSMKDRFAKLNLSVSEVDD
                     NDKWQSAVLGLALVTNDAGFASSVMDRVILDMEDAYEAEMVDVKREIIHL"
     gene            1057..1422
                     /gene="rbfA"
                     /locus_tag="PLT69_05680"
     CDS             1057..1422
                     /gene="rbfA"
                     /locus_tag="PLT69_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOJ13963.1"
                     /translation="MKVVRLRQARVGDLVGRTIAELIQKKVKDPRLEGVTITGADVSV
                     DLKAARIYFCVMGRERKGSVLEGLASAAGYLRRELGRELHMKAVPSLSFAYDESFDHG
                     ARIDEILDSLASHQDDGKE"
     gene            1436..2371
                     /locus_tag="PLT69_05685"
     CDS             1436..2371
                     /locus_tag="PLT69_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOJ13964.1"
                     /translation="MEMKSKIARILRERDSFEILTHVQPDEDAVGSTRALGLALQAMG
                     KAVDIVYPDPVPRSLDFTEAPIRADGTNPAVSIVVDLSDPVLLGGVNPRGEVIVIDHH
                     RGEGLFGAFRWIDPTRSSSAEMVYELLCTLETDITPAIATNLYMGLFGDTGGFMHANT
                     TKEVFGIAHDLVSKGADPHAVAYRIKKTRALAFYKLLCAAVNRLVIRDRVLASYVTHE
                     EITRFGARAEDTSGIVEEMASLDGVDLVIFLKEVSRGTIKASLRSRVKDAALNTAAAF
                     GGGGHGLAAGCTLSGTPEAVVVEMIEEGVKWVRTA"
     gene            2353..3246
                     /gene="truB"
                     /locus_tag="PLT69_05690"
     CDS             2353..3246
                     /gene="truB"
                     /locus_tag="PLT69_05690"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ13965.1"
                     /translation="MGSDGIVLIDKPAGITSRKAVDRVMAALGVKKAGHFGSLDPFAT
                     GLLCIGVGQGTKLLPFLQDHPKEYIALIGFERFTDTDDITGQVTASFEGVRVDPGGIR
                     AWFDANRGTIRQVPPVYCAQKHLGKPMYKLKRANKEVSPRPKEVHLFETEILGCGDDW
                     VEVRVVCSRGTYIRSIARDLGTHLGVGGYLRRLKRTKSEGFSLDDAHAIDALVALGDK
                     AVIPLGEALNIPKARVTKVGAMGIRDGMPIQISWVKDDIEVAQGGLAALTDSEGSLLC
                     IARIRREGGIWGYIERGFKVY"
     gene            3366..3635
                     /gene="rpsO"
                     /locus_tag="PLT69_05695"
     CDS             3366..3635
                     /gene="rpsO"
                     /locus_tag="PLT69_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ13966.1"
                     /translation="MALQAEEKKRIIDQFKRHDKDTGSPDVQVAILTKRINDLTEHFK
                     THKKDHHSRRGLLMLVGQRRRLLNYLRDHDIKRYRDLVQTLNLRK"
     gene            3661..>4297
                     /locus_tag="PLT69_05700"
     CDS             3661..>4297
                     /locus_tag="PLT69_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ13967.1"
                     /translation="MKIQAKGTDTPLIFEVGTVARQADGAVLVHQGDTVVLVTATVAK
                     EERKGVDFLPLVVDYQEMSYAAGRIKGGFIKRDGRPGTQEILTARCIDRPIRPLFPHT
                     FRNEIQIIAMVLSADPDWTPDVMAVNGASAALTARVGLIDGRIVIDPSVAALENSKLD
                     LLVVGTKDAIVMVEGEASEVSEDTVIEAISFAHKRINDVVEAIEELGRSFGK"
BASE COUNT          941 a         1196 c         1433 g          727 t
ORIGIN      
        1 caggcgatac catgttcgtg gtgccgagcg agaagaaggc caaggagatc gtcgagtatc
       61 tccagaacaa ggagatggta tcgaggctcc aggcgcccga gcagaagccc caggtcaccc
      121 tggaggatct ctactcgcag gtgcaggaag ggaagatcaa ggatctcaac ctcatcgtca
      181 agggggacgt acacggcacc gtcgaggcga tcgtgagctc ggtgaagggc atcgacacct
      241 cgggccacga catcaggatc aatgtgatcc actcggcggt cgggggtatc accgagaacg
      301 acgtgctcct cgcctccacg tcgtcggcga tcatcgtcgg gttcaacgtg aggccggaga
      361 ccaaggccag ggacctggcg aagaagcaca aggtcgagat caagctttac accgtcatct
      421 acgacctcat cgaggacatc aagcaggcgc tcaggggtat gctggagccc gtctacaggg
      481 aggtggtcct cggccgcgcg gaggtgaggg agatattcaa gatacccaag gtgggggtcg
      541 tcgccgggtg catggtcact gaaggcacca tccaacggac gtcgagcgtc aggctcctca
      601 gggacaacgt catcgtccac gaggggaagg tggattcgct caggcggttc aaggatgacg
      661 tccgcgaggt ccagatgggc tacgagtgcg gtatcgggct cggcgggtac aacgacataa
      721 aggtggggga tgtcatcgag gccttcacca tggagaaggt ggagacgaca agctcgtggt
      781 gatcgcggcg ctggaagttc gtctcaggat accgggtgcg cattccctca aggagaagcg
      841 cagggtgatc aagtccatga aggacaggtt cgccaagctg aacctctccg tctccgaggt
      901 ggacgacaac gacaagtggc agtcggctgt cctggggttg gccctcgtca cgaacgacgc
      961 gggattcgcg agctcggtca tggacagggt catcctcgac atggaggatg catacgaggc
     1021 cgagatggta gacgtgaagc gggagatcat ccacctatga aggtcgtgag gctgcgccag
     1081 gccagggtgg gcgacctggt gggacggacc attgcagaac tcatccagaa aaaggtcaag
     1141 gacccgcggc tggagggggt gaccataacc ggggcggacg tgagcgtcga cctgaaggcg
     1201 gctcgcatct acttctgcgt tatgggcaga gagaggaagg gatccgttct cgagggtctc
     1261 gcgagtgccg ccgggtacct ccgcagggaa ctggggaggg aactccacat gaaggccgtc
     1321 ccctccctga gcttcgctta cgacgagtcg ttcgatcacg gggcgaggat cgacgagatt
     1381 ctcgattcct tggcatcgca ccaagacgac ggaaaggaat gaatcggcgc ctggaatgga
     1441 gatgaagtcc aagatcgcgc gcatcctcag agaacgcgac tcgttcgaga tcctcactca
     1501 cgtgcagccg gacgaggacg ccgttgggtc cacccgggcc ttggggctcg ccctccaagc
     1561 catgggaaag gccgtcgaca tcgtgtaccc ggaccctgtt ccccgatcgc tggattttac
     1621 cgaggcgccc atacgggcag acggaaccaa ccccgcggtt tccatcgtcg tggatctttc
     1681 agaccccgtc ttgctcgggg gcgtgaaccc ccgtggagag gtgatcgtca tagaccacca
     1741 caggggagag ggtctcttcg gtgctttccg ctggatcgat ccgacacggt cgtcctcggc
     1801 ggagatggtc tacgaactct tgtgcaccct cgaaacggac ataacgccgg ctatcgcgac
     1861 gaacctgtac atgggcctct tcggggatac gggggggttc atgcacgcga acaccacgaa
     1921 ggaggtcttc ggcatcgccc atgacctggt ttccaagggc gcggaccccc atgcggtggc
     1981 gtaccggatc aagaagacga gggcccttgc cttctacaag ctcttgtgtg ccgcggtcaa
     2041 tcggctggtc atcagggacc gggtgctcgc gagctacgtc acccacgagg agatcacgcg
     2101 tttcggggcc agggccgagg acacatccgg gatcgtggag gagatggcct ccctggacgg
     2161 ggtggatctc gtgatctttc tcaaggaagt ctcccgcgga acgatcaagg cctctctgag
     2221 gagcagggtg aaggatgcgg ccctcaacac ggcggccgct ttcggcgggg gaggccatgg
     2281 gctcgccgct gggtgtaccc tcagcggaac ccccgaggct gtggtagtgg agatgatcga
     2341 ggaaggtgtc aagtgggttc ggacggcata gtgctcatag acaagcccgc cgggatcacc
     2401 tccaggaagg cggtggacag ggtcatggct gcgctggggg tgaagaaagc gggtcacttc
     2461 gggtctctcg acccctttgc cacagggctc ttgtgcatcg gcgtggggca ggggacgaaa
     2521 ctcctcccgt ttctccagga tcatcccaag gaatacatcg ccctcatcgg gttcgaacga
     2581 ttcaccgaca ccgatgacat aaccggccag gttaccgcca gcttcgaggg ggtgagggtc
     2641 gaccccggcg gcatacgggc ttggttcgac gccaacaggg ggacgatcag gcaggtgccg
     2701 cccgtttatt gcgcacagaa acacctgggg aagccgatgt acaagctgaa gcgggcgaac
     2761 aaggaggtaa gccccaggcc gaaggaagtc cacctgttcg agacagagat cctcgggtgc
     2821 ggcgatgatt gggtggaggt tcgcgtggtg tgctcccgcg ggacctacat caggtccatt
     2881 gcgagggatc tggggactca cttgggcgtt ggaggctatc tcaggcgtct caagcgcacg
     2941 aagagcgagg ggttctccct ggacgatgcc cacgccatcg acgccctcgt cgccctgggc
     3001 gacaaggcgg tgatacccct tggagaggct ttgaacatcc ccaaggccag ggtgacgaag
     3061 gtcggcgcaa tgggtatacg ggacggcatg cccatccaga tctcctgggt gaaagacgac
     3121 atcgaggtcg cccagggggg gctggcggcg ctcacggact ccgagggaag cctcctctgc
     3181 attgccagga tccggaggga aggcggaatc tgggggtaca tagagagagg cttcaaggtt
     3241 tattgactat ttacagcaga aacgaaggtg tggtaatgtc tccaccttgt tcacggatac
     3301 acgcatgcag gaatgtcccg tgttcggcgg accaagatcg cacctaacgc aggaggaacc
     3361 gagaagtggc attgcaggca gaagagaaaa agcggatcat cgatcaattc aagcggcatg
     3421 acaaggacac cggctcacca gacgtacagg tggccatcct cacgaagagg ataaacgacc
     3481 tcaccgagca cttcaagacg cacaagaagg accatcactc gaggaggggg ctgctcatgc
     3541 tcgtcggcca gaggaggcgt ctgctcaact atctgcgcga tcacgacatc aagcgataca
     3601 gagacctcgt acagaccctg aacctcagaa aataacgtac ggagaaggca tacaaacgac
     3661 atgaaaatac aggctaaagg aaccgatacg ccgttgatct tcgaggtggg gaccgttgcc
     3721 aggcaggcag acggggcggt gcttgtccac cagggagaca cggtggtcct cgttaccgcg
     3781 accgtcgcca aggaggaacg caagggcgtg gatttcctcc ccctcgtcgt ggattatcag
     3841 gagatgagct acgcggcggg aaggatcaag ggcgggttca taaagcgtga cggccggccc
     3901 gggacgcagg agatcctcac ggcccgttgc atcgacaggc ccatcaggcc cctgttcccc
     3961 cacaccttcc gaaacgagat acagatcatc gccatggtgc tgtcggcgga ccccgactgg
     4021 accccggacg tcatggcggt caacggcgca tctgcggcgc tgaccgcccg ggtggggctc
     4081 attgacggca ggatcgtcat cgacccctct gtggcggccc tcgagaacag caagctggat
     4141 ctcctggtcg tgggcacaaa ggatgccatt gtcatggtgg agggcgaggc cagcgaggtc
     4201 tccgaggata ccgtgatcga ggcgatcagc ttcgcccaca agcgcatcaa cgacgtcgtg
     4261 gaggcgatcg aagagctcgg gaggtccttc ggcaagg
//
LOCUS       DAOSCR010000181         4292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12930_length_4292_cov_3.410432, whole genome shotgun sequence.
ACCESSION   DAOSCR010000181 DAOSCR010000000
VERSION     DAOSCR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12930_length_4292_cov_3.410432"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /gene="hisD"
                     /locus_tag="PLT69_05705"
     CDS             <1..909
                     /gene="hisD"
                     /locus_tag="PLT69_05705"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014716941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ13968.1"
                     /translation="PGGANAFPSTMLMNVIPARIAGVGRIEVVSPTPKGRVNEALLAA
                     AHASGVDRIVRIGGAQAVAALAYGTERVTRVDKVVGPGNIYVAHAKRLLQGVIGIDSF
                     AGPSEILVIADEHADPSVVANDLLSQAEHDAMASAILVTPSEPLLQGVLAAIERALET
                     LPRREVIEKSLDHHGACIVVRDLDEAVEVANVVAPEHLELMVQDAWSYLGGIRNAGAV
                     FLGYHTPEAIGDYVAGPNHTLPTSSTARFSSPLGVYDFTKRTSVVAVSRKALETLGPA
                     AVLLARSEELEAHARSVECRLGKPHP"
     gene            866..2161
                     /locus_tag="PLT69_05710"
     CDS             866..2161
                     /locus_tag="PLT69_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13969.1"
                     /translation="MRDLSSAASASPILERILPLLPQDAYVVGGCVRDLVLGVAPTDI
                     DLVTFGDVEALATRIAQAFYAHPFVMDPERRVIRVALRGGRATVDFTPGRGKSIEDDL
                     LERDITVNAMAWHPDSPGVVDPLGGLEDIEKRVIRLVREENLLDDPIRGLRCLRFAVQ
                     LGFSLDPATAELVRKHAKTLSAVAPERIKHELSRALAMPSGGALFPLVVDAGMDGVLF
                     GRESKAGSIRSLEAAARAEDVLGRSESVLPGSARVLGGELESGFTRKAALRLVSFLAG
                     DGQAGDAVFGVSSAARRLALSTRARRVMRETVEGALTAARGRPPGLDGAAWMHRLAAS
                     YGAVLPELLIVCLAWGKGPDEALARRIWEYARGPYLEQAARPLLRGSDIIEHLGVPPG
                     PTIGELLSLLGEARAAGLVSNREEALAFLREHLGVSKNT"
     gene            2259..2858
                     /locus_tag="PLT69_05715"
     CDS             2259..2858
                     /locus_tag="PLT69_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ13970.1"
                     /translation="MKVESWMVRDVVTIASHAGIREALTVMKSHSVRHLPVVDGGRLV
                     GLVTLGDLKQAILASMIEQLLVRDVMVRDPYTTTKDTPLEKAAQLIYEQRIGCLPVVE
                     EGSVVGILTVNDILKAFIDIMGVLKAGSRIDVILKNVHGSFDEVVGIIEDKGGYIISA
                     GMTMNGDDNVHHFRVSGGDTEAMARALTTLGYKGVKVID"
     gene            complement(2848..3138)
                     /locus_tag="PLT69_05720"
     CDS             complement(2848..3138)
                     /locus_tag="PLT69_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOJ13971.1"
                     /translation="MWCSERGDASFLNVRVKPGSSRQGIGPVENDLLTVRLNAPAVEG
                     RANEALVTLLAKALGVAKTKIEIVTGEKGRVKKVRVEGLTAREVASRLDISL"
     gene            complement(3125..3640)
                     /locus_tag="PLT69_05725"
     CDS             complement(3125..3640)
                     /locus_tag="PLT69_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOJ13972.1"
                     /translation="MKLSPEMIREQRFRTRFKGFDRQEVTAFLLDLAEDMESLIEENS
                     LLKGEIEEGKKRQREIEDMILSLRQFTEERMKMAESESSALVDKAERRASEIQEAASR
                     KMAEAEQRAQEVLAQAMRKAKEMLRDAERARQEAERSLEEISERRKAMLEQLRTVVDS
                     CRAWIDAHVVQ"
     gene            complement(3641..3949)
                     /locus_tag="PLT69_05730"
     CDS             complement(3641..3949)
                     /locus_tag="PLT69_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOJ13973.1"
                     /translation="MYILGNILIGLGNVLDILLSVYMWIVIVGALISWVNPDPYNPIV
                     RFLRSATYPVFAWVRSRLRLTAGGIDFSPVVVIAAIVFLRYALARSIVEAGIRLKGGF
                     "
     gene            4082..>4292
                     /locus_tag="PLT69_05735"
     CDS             4082..>4292
                     /locus_tag="PLT69_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ13974.1"
                     /translation="MRLSRIEIRGFKSVARKTALVISDGVTCIAGPNGCGKSNIVDAV
                     RWALGEQSPKALRASSMNDVIFSGTQ"
BASE COUNT          755 a         1392 c         1371 g          774 t
ORIGIN      
        1 cccggcgggg ccaacgcctt cccctcgacc atgctcatga acgtgatccc ggccaggatc
       61 gccggtgtgg gccggatcga ggtggtctcc cccacgccca agggacgggt gaacgaggcc
      121 ctgctcgcgg ccgctcacgc ttcaggcgtg gacaggatcg ttcgcatagg cggcgcgcag
      181 gccgtggcgg ccctcgctta cggtacggaa cgcgtcacga gggtggacaa ggtggtgggg
      241 cctggcaaca tctacgtggc gcacgccaag aggctcctcc agggcgtcat agggatagac
      301 tcgttcgcag gtccctccga gatcctcgtg atcgcggacg agcacgcgga tccgtcggtt
      361 gtggccaacg acctcctgtc ccaggcggaa cacgacgcca tggcctcggc catcctcgtc
      421 accccttcgg aacccctcct ccagggtgtg ctcgcagcca tcgaacgtgc tctcgaaacc
      481 ctgccgagaa gggaagtgat cgagaagtcc ctcgatcatc acggcgcgtg catcgtcgtg
      541 agggacctcg acgaggccgt ggaggtcgcc aacgtggtgg cgccggaaca cctggagctc
      601 atggtccaag acgcgtggtc gtacctcggg ggcatacgga atgcgggcgc cgtgttcctg
      661 gggtatcata cgcccgaggc cataggcgac tatgtcgcgg gccccaacca cacgctgccg
      721 accagctcaa ccgcacggtt ttcatccccg ctcggggtgt acgacttcac caagcggacc
      781 tcggtcgtcg ccgtgagcag aaaggccctt gaaaccctgg ggccggcggc ggtgctcctg
      841 gcccggtcgg aggagctcga ggcacatgcg agatctgtcg agtgccgcct cggcaagccc
      901 catccttgag cggatcctgc cccttttgcc acaagacgcg tacgtggtgg gagggtgcgt
      961 gcgagacctc gtgctgggtg tcgcgccgac ggacatagac ctcgtcacct tcggggacgt
     1021 cgaggccctc gccacaagga tcgcacaggc attctacgcg caccccttcg tcatggaccc
     1081 cgagcgcagg gtcatccgcg tcgcgctcag gggaggcaga gccaccgtgg acttcacccc
     1141 gggccgcgga aagtccatcg aggacgatct cctcgagagg gacatcacgg tcaacgccat
     1201 ggcctggcac cctgactccc ccggtgtcgt cgatcccctc ggggggctcg aggacatcga
     1261 gaagagggtg atcaggctcg ttcgcgaaga gaatctcctc gacgacccga tacgggggct
     1321 caggtgcctt cgattcgccg tgcagctggg gttctccctc gatccggcca cggccgagct
     1381 cgtcaggaag cacgcaaaga ccctatcggc cgtggcgccc gagaggatca agcacgagct
     1441 ctcgagggcg cttgcgatgc cttcaggcgg cgcgctcttc ccgctcgtgg tggatgccgg
     1501 gatggacggg gtgctgttcg ggcgtgaatc aaaagccgga tccatccggt cgctcgaagc
     1561 ggccgccagg gccgaggacg ttcttgggcg gtcagagagc gtcctgcccg ggtcggcccg
     1621 tgtccttggc ggggagctcg agagcgggtt cacgaggaag gcggccctca ggctcgtctc
     1681 tttcctcgca ggagacgggc aagcaggcga cgccgttttc ggcgtgtcat ccgcggccag
     1741 gaggctcgcc ctctcgacga gggcccgaag ggtcatgagg gagaccgtgg aaggggcgct
     1801 cacggcggca cgcggtcgtc ccccggggct ggacggcgcg gcatggatgc accggttggc
     1861 ggcgtcatac ggcgccgtcc tgccggagct gctcatcgtg tgcctcgcgt gggggaaagg
     1921 gccggacgag gcgctcgccc ggcgtatctg ggaatacgcc cgcgggcctt acctcgagca
     1981 ggcggcccgc ccgctcctca ggggatcgga catcatcgag cacctggggg taccgccggg
     2041 ccccactatc ggagaactcc tgtcgctcct cggggaggcc agggccgcag gccttgtgtc
     2101 gaaccgcgag gaagcccttg cgttcctcag ggagcacctg ggggtatcga aaaacacgtg
     2161 acgatccctt gcggggagac cctgccgttg cccgtaaacg gccttatcct gcggcttgtt
     2221 ggtgtacaat cgacgtcgtc tctggtatat gaacgggtat gaaggtagag tcctggatgg
     2281 tcagggacgt ggtcacgata gcaagccacg caggcatccg cgaggccctt accgtgatga
     2341 agagccactc tgtcaggcac ctgccggtgg tcgacggcgg caggctcgtc gggttggtga
     2401 cgctcgggga cctcaagcag gcgattctcg cctccatgat cgagcaactc ctggtgaggg
     2461 acgtgatggt acgcgacccg tacaccacca ccaaggacac cccccttgag aaggccgctc
     2521 agctcattta cgaacagagg ataggctgcc tccccgtggt cgaggagggc tctgtcgtgg
     2581 gcatcctcac ggtcaacgat atcctcaagg ccttcatcga catcatgggc gtgctgaaag
     2641 cgggaagcag gatcgacgtg atcctcaaga acgttcacgg atcgttcgac gaggtggtgg
     2701 gcatcatcga ggacaagggc ggctacatca tcagcgccgg catgaccatg aacggtgacg
     2761 acaacgtcca ccatttccgt gtctccgggg gggacacgga ggccatggcc agggcgctca
     2821 ccaccctcgg atacaagggg gtgaaggtca tagactgatg tcgagcctcg aggcgacctc
     2881 cctcgccgtc agcccctcga ccctgacctt cttgaccctg cctttctcgc cggtgacgat
     2941 ctcgatcttc gtcttcgcga cgcccagggc cttggccagg agcgtcacca gcgcctcgtt
     3001 ggccctgccc tccacggccg gcgcgttcag cctgaccgtg aggaggtcgt tttccaccgg
     3061 tccgatcccc tggcgggacg accccggctt cacgcggacg ttcagaaaag aagcgtcccc
     3121 tctctcactg caccacatgg gcgtctatcc atgccctgca tgagtccacc accgttctga
     3181 gctgctccag catggccttt cgcctctcgg aaatctcctc gagagacctt tccgcctcct
     3241 gcctcgccct ctccgcatcc ctgagcatct ctttggcctt cctcatcgcc tgggcgagaa
     3301 cctcctgggc gcgctgctcg gcctccgcca tcttccgaga cgcggcctcc tgtatctcgg
     3361 aggccctcct ttcggccttg tcgaccaagg ccgacgactc ggactcggcc atcttcatgc
     3421 gctcctcggt gaactgtctc aaagacagga tcatgtcctc gatttccctc tgcctcttct
     3481 tgccctcctc gatctccccc ttgaggaggg aattctcctc gatgagcgat tccatgtcct
     3541 cggcgaggtc cagcaggaat gccgtcacct cctgccggtc gaagcctttg aagcgggtcc
     3601 tgaagcgctg ctcgcggatc atttcgggag agagcttcat ctagaacccc cctttcaggc
     3661 ggatgccggc ctccacgatg gaccgcgcga gggcgtacct gaggaagacg atcgccgcga
     3721 tcaccacgac gggcgagaaa tctatgcccc ccgccgtgag cctcagcctc gatctcaccc
     3781 atgcaaagac cggatacgtg gccgagcgga gaaacctgac gatggggttg tagggatcgg
     3841 ggttgaccca cgagatcaga gccccgacga tgacgatcca catgtacacg ctcaggagga
     3901 tatccagaac attgccgagc cctatgagga tgttgcccag gatgtacatg ccgtgccccc
     3961 ctcgatcgat ctcgatggat aatgtgccgt tttctctccc tgaaagcaag gggattcgag
     4021 ggattcccac cctgggaagc cggtcttgcc tagggggtga agctctgata ttgcatggtt
     4081 cgtgagattg tctcggattg agatcagggg cttcaagtcg gtcgcgagaa agacagccct
     4141 ggtcatctcc gacggggtga cctgcatagc ggggccgaac ggctgcggaa agagcaacat
     4201 cgtggacgcc gtgaggtggg ccttgggaga gcagtcaccc aaggccctca gggcctcgtc
     4261 catgaatgac gtcatctttt ccggcaccca ag
//
LOCUS       DAOSCR010000182         4288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_12952_length_4288_cov_3.885897, whole genome shotgun sequence.
ACCESSION   DAOSCR010000182 DAOSCR010000000
VERSION     DAOSCR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12952_length_4288_cov_3.885897"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLT69_05740"
     CDS             complement(<1..415)
                     /locus_tag="PLT69_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ13975.1"
                     /translation="MQPKTTILSLPEEEFVNPGTRACAGCGLGIAYRVALKAVGRDAI
                     LIVPPSCLTVLQGLYPVAATRLPCLNVTFASTAAAATGMVAALKALGRKDTRVVAWAG
                     DGGTADIGLQALSGAAERGDDFLFICYDNKGYMNTG"
     gene            complement(426..1637)
                     /locus_tag="PLT69_05745"
     CDS             complement(426..1637)
                     /locus_tag="PLT69_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOJ13976.1"
                     /translation="MSSHIIESGNTAAALGVKLCRVQVIAAYPITPQTPLTEKLSELV
                     DSQEMEAQYIPVESEHSALAVCIAASSTGARAFTATSANGLLYMHEQIHWAAGARLPL
                     VMCVVNRGVGAPWTIWNDHQDSISQRDTGWIQLYVNDHQQIIDGVIKAYRLAESVMIP
                     VMVCYDGYLLSHTHMPFEVPSQEAVDSFLPPYRPDYALDPKRPANLNTVTLPDTRVDT
                     EGRTAHGYMEIRSLLHEDLQRAVEVYQDVEAGFRDTFGRGGEALFEHYRCEDAEYVAV
                     ALGSLSHNLRDVVDTLREAGVPAGVMSIHLYRPFPARQIASFLSPAKGVIVFEKALSY
                     GYEGALCSDIKAALLTHARSRADLPFVHNYILGLGGREIKTSQLVEAMTASCKAGAAS
                     HDGVRWIGLKL"
     gene            complement(1634..1948)
                     /locus_tag="PLT69_05750"
     CDS             complement(1634..1948)
                     /locus_tag="PLT69_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ13977.1"
                     /translation="MGTRTYPRKTSHSEPGPGDGGRTGSWRVARPVIDPQVCTPSRRG
                     VPSCFICWLYCPDGVISRTVPPKIDYEYCKGCGICAEECPTKAISMVDEACFIEEGRD
                     TT"
     gene            complement(1951..2508)
                     /locus_tag="PLT69_05755"
     CDS             complement(1951..2508)
                     /locus_tag="PLT69_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ13978.1"
                     /translation="MKDTIDIRWHGRGGQGAITAAKIVAKAMFSLGYEGVAMIPTFGT
                     ERRGAPVFSSLKISRRKIYDLSPIERPDIVVVLDHVLMEEADITSGLKEGGLLVINSP
                     CSLESYHADGRKVAVCNVTALATRAGLPAGVVNTGIIGAFARASGLAGLDLLTEVIEE
                     EFKGRKPEANALAARLAYEHTIVGG"
     gene            2711..3685
                     /locus_tag="PLT69_05760"
     CDS             2711..3685
                     /locus_tag="PLT69_05760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ13979.1"
                     /translation="MIVHEASRTEPGRRGADRHGPARPLALYTEGIMILLKGSDTHTA
                     LVDLVSLMAQSFPLSAPAVYVVTRGRQARAFSTLDPLDAGQADAMRVLGKAPWSYERV
                     IAAGQAPLAPERVDIELVGGEKARVILSVNAGNHVDIFLEWAKYLTAAIAKILDNEML
                     QDMAFTDGLTGLLNYRAFMGILNAECERARRYDTTFSLMMLDIDHFKLINDTFGHPAG
                     DRILVALAERLQGRLRKSDLVFRYGGEEFMAILPQTGMYKAAFLAERIRTFVERMRTE
                     DGMSVTVSIGVSQYHRDLSPGDLIRQVDAGLYLAKGKGRNRVEIYAAR"
     gene            3893..>4288
                     /locus_tag="PLT69_05765"
     CDS             3893..>4288
                     /locus_tag="PLT69_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13980.1"
                     /translation="MNIDSLLNLDAGITAMNAQGERARVRVGEILEDDIFVLVPEGEF
                     HAEEGGIVKVTDGQDSVLAKVVERSGDEIRLCMECYLSPGHERRSDVRIYDRVYYDVK
                     FICHASEKAGRLHEALDRIRAHKTVLDSFV"
BASE COUNT          714 a         1355 c         1473 g          746 t
ORIGIN      
        1 ccccggtgtt catgtagccc ttgttgtcgt agcagatgaa caggaagtcg tccccccgct
       61 cagccgcacc ggagagggcc tgcagcccga tgtccgcggt ccccccgtcg ccggcccacg
      121 ccacgacgcg cgtgtctttc ctgcccaggg ccttgagcgc cgccaccatg ccagtggcgg
      181 ccgccgcggt ggaggcgaag gtaacgttca ggcagggcag cctggtcgct gcgaccgggt
      241 agaggccctg cagaacggtg agacagctcg gaggaacgat gaggatggcg tctctgccca
      301 cggccttgag cgcgacccgg tacgcgatgc cgaggccgca cccggcgcag gcccgcgtcc
      361 ctgggttgac gaactcctcc tcgggcaggc tcaggatggt tgtcttgggc tgcatgcggc
      421 tccccctaga gcttgaggcc tatccagcgc acgccgtcgt ggctcgcggc gccggccttg
      481 caggaggcgg tcatggcctc cacgagctgg gacgtcttga tctccctgcc gcccaggccc
      541 aggatgtagt tgtggacgaa gggcaagtcg gcccgggacc gcgcgtgcgt gagcagggct
      601 gcctttatgt ccgagcacag ggccccctcg tacccgtagc tcagcgcctt ctcgaagacg
      661 atcaccccct tggccggcga gaggaaagag gcgatctgcc gtgccgggaa gggccggtag
      721 aggtggatgg acatcacgcc ggcagggaca cccgcttccc tcagcgtgtc caccacgtcc
      781 ctcaggttgt gggagaggga cccgagggcc acggccacgt actcggcgtc ctcgcagcgg
      841 tagtgctcga agagggcctc gccgccgcgg ccgaaggtgt ccctgaagcc ggcctccaca
      901 tcctggtaga cctccacggc ccgctgcagg tcctcgtgga ggaggctgcg gatctccatg
      961 tagccgtgcg ccgtcctgcc ctcggtgtcg acgcgggtgt ccggcagcgt aaccgtgttc
     1021 aggttcgcgg gtcgtttcgg gtccagcgcg tagtccggcc ggtagggcgg gaggaacgag
     1081 tccacggcct cctgggaagg gacctcgaag ggcatgtggg tgtgtgacag gaggtacccg
     1141 tcgtagcaca ccatgacggg gatcatgacc gactcggcga gccggtaggc cttgatgacc
     1201 ccgtctatga tctgctggtg atcgttgacg tagagctgga tccagccggt gtccctctgg
     1261 gagatggagt cctggtggtc gttccagatg gtccagggcg cgccgacgcc cctgttcacc
     1321 acgcacatga ccagggggag ccgcgcgccc gcggcccagt ggatctgctc gtgcatgtag
     1381 aggaggccgt tggcgctcgt cgccgtgaac gcccgtgcgc cggtggagga tgcggctatg
     1441 cagacggcga gcgcgctgtg ttcgctctcc accgggatgt actgcgcctc catctcttgg
     1501 gagtcgacca gttccgagag cttctcggtg agcggggtct gcggtgtgat ggggtaggcg
     1561 gcgatgacct gcacccggca cagcttgacg ccaagggccg cggccgtgtt ccccgattcg
     1621 atgatgtgcg agctcacgtg gtgtccctgc cttcctcgat gaaacatgcc tcgtcgacca
     1681 tggagatggc cttggtgggg cactcttctg cgcagatgcc gcaccccttg cagtactcgt
     1741 agtcgatctt cggggggacg gtcctgctga tgacgccgtc ggggcagtag agccagcaga
     1801 tgaagcacga ggggacgccg cgcctggatg gggtgcagac ctgggggtcg atcaccgggc
     1861 gggcgaccct ccaggaaccg gtcctcccgc cgtccccggg gccgggctcg ctgtgggagg
     1921 tcttgcgggg gtacgtgcgg gttcccatgg tcaacccccg acgatcgtgt gttcgtacgc
     1981 gagccttgcg gcgagcgcgt tggcctcggg tttcctgccc ttgaactcct cctcgatgac
     2041 ttccgtgagg agatcgagac ccgccagacc ggacgcgcgg gcgaaggcgc ctatgatgcc
     2101 ggtgttgacg acgcctgcag gcaggccggc cctcgtggcg agcgcggtca cgttgcacac
     2161 ggccaccttc ctgccgtccg cgtggtacga ttcgaggctg cacggcgagt ttataacgag
     2221 gaggccgcct tccttgagac ccgaggtgat gtccgcctcc tccatgagca cgtggtcgag
     2281 cacgaccacg atgtcgggtc gctcgatggg cgaaaggtcg tagatcttgc ggcgcgagat
     2341 cttcagggag gagaacacgg gcgcgccccg ccgttcggtg ccgaaggtgg gtatcatggc
     2401 cacgccctcg tagccgaggc tgaacattgc cttggcgacg atcttggccg cggtgatggc
     2461 accctggccg cccctgccgt gccacctgat gtctatggtg tccttcatgg aagtcccctc
     2521 gctgccgtag ctgtccccgt gatcgcgcgg gtatggcggc cgtcatgccc ctgtgcccgt
     2581 ccatcgcgcg aacggcatgg gatgtccgtt caccggggga actatatacc aagtgagagg
     2641 ccgaatcaag gcacatggat tgcatggcaa tcgaatggca accgaaacca ggacagagaa
     2701 aggatcgcag atgatcgttc acgaggcgtc caggacggaa ccggggaggc ggggtgccga
     2761 caggcatggc cccgccaggc cgctggcctt gtataccgag ggcatcatga tcctgctcaa
     2821 ggggtccgac acgcacaccg cccttgttga cctggtctcg ctcatggcgc agagtttccc
     2881 gctgtccgcc ccagccgtct acgtggtcac caggggcagg caggcacgcg ccttctccac
     2941 gctcgacccc ctggatgcgg gccaggcgga cgccatgcgc gtactcggaa aggccccatg
     3001 gtcctacgag cgcgtgatcg ccgctggaca ggctcctctg gcgcccgagc gcgtcgacat
     3061 agagctcgtg ggcggggaga aggcgagggt gatcctctcg gtgaacgccg gcaaccacgt
     3121 cgacatcttc ctggagtggg ccaaatattt gacggccgcg atcgccaaaa tattggacaa
     3181 cgagatgctc caggacatgg cgttcacgga cgggctcacg ggactgctca actaccgggc
     3241 cttcatgggc atcctgaacg ccgagtgcga acgggcgagg agatacgaca cgacgttctc
     3301 cctcatgatg ctcgacatag accacttcaa gctcatcaac gacacgttcg gtcaccccgc
     3361 cggcgacagg attctcgtcg cgctggcgga aaggctccag ggacgccttc gcaagagcga
     3421 cctcgtcttc aggtacgggg gagaggagtt catggccatc cttccccaga cggggatgta
     3481 caaggccgcg ttcctcgcgg agcgcatcag gacgttcgtg gagcgcatgc gcaccgagga
     3541 cgggatgtcc gtgaccgtga gcatcggcgt gagccagtac cacagggacc taagccccgg
     3601 tgacctcatc cggcaggtcg atgcgggtct ctatcttgcc aagggcaagg gacgcaaccg
     3661 ggtcgagatc tatgccgcca ggtgatggat ctcgcccgat gtccgtcttc ggtccggacg
     3721 ccgtctccac tggattccag tgccgtcctg tgatagggga gcgaaaggcc gctggcacgg
     3781 gagacggcct cgcggggagg cggcgcccgg aagtctcgcc cggccgcagg gggccgtccc
     3841 aagggtggtg ccgcccgagg ccatgccgcc ttgaattcga ggagcccatg cgatgaacat
     3901 tgacagcctt ctcaacctgg atgcagggat cacggccatg aacgcccaag gtgaacgggc
     3961 aagggtcagg gtgggggaga tcctcgagga cgacatcttt gttctcgtgc ccgagggtga
     4021 gttccatgcc gaggagggcg gcatcgtcaa ggtgaccgac ggccaggact cggtgctcgc
     4081 caaggtcgtg gagaggtcgg gcgacgagat ccgtctctgc atggagtgct acctctcccc
     4141 tggtcacgag cggagatcgg acgtgcgcat ctacgaccgc gtgtactacg acgtgaagtt
     4201 catctgtcat gcctccgaga aggccgggcg cctgcacgag gcgctcgacc gcatccgggc
     4261 ccacaagacc gtgctcgatt ccttcgtc
//
LOCUS       DAOSCR010000183         4276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13006_length_4276_cov_2.982232, whole genome shotgun sequence.
ACCESSION   DAOSCR010000183 DAOSCR010000000
VERSION     DAOSCR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13006_length_4276_cov_2.982232"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLT69_05770"
     CDS             <1..840
                     /locus_tag="PLT69_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ13981.1"
                     /translation="LDRLVFSKIRERFGGRLRGAMTASAAMNPEIAGFFFDIGIPTYD
                     CYGMTETSPAVTMNCPSAYKLGSVGRPIDKVKVVIDSSVVEEGAEDGEIVVYGPNVMK
                     GYHNKPEATAAVMTPDGGIRTGDRGRFDKDGFLYITGRIKEQYKLENGKFVFPAALEE
                     DIRLIPYIENAMVYGENKPYNICIVIPDFAVLGKYARENGLPTEPEELVRRKEVYDLI
                     ADEVNKALKGKYGGYEIPKKFLILSQNFSVDDGTLTQTLKVKRRVVIQRLKDSIEALY
                     EKK"
     gene            complement(1147..1362)
                     /locus_tag="PLT69_05775"
     CDS             complement(1147..1362)
                     /locus_tag="PLT69_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOJ13982.1"
                     /translation="MAESVYKVIEIIGTSPVSWEKAAEAAVATASKTIKDLRIAEVVE
                     LDLQIEDGKVVNYRTKLSLSFKYLGKD"
     gene            complement(1444..>1524)
                     /locus_tag="PLT69_05780"
     CDS             complement(1444..>1524)
                     /locus_tag="PLT69_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13983.1"
                     /translation="ARLRVSFRYHMIFSLTPKYHQKGARW"
     assembly_gap    1525..1534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1535..1704)
                     /locus_tag="PLT69_05785"
     CDS             complement(<1535..1704)
                     /locus_tag="PLT69_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOJ13984.1"
                     /translation="MAEREYQVIELVGTSPVSWEKAAETAVAEASETLTDLRIAEVVR
                     LDMHADEEGRPL"
     gene            complement(2373..2807)
                     /gene="arfB"
                     /locus_tag="PLT69_05790"
     CDS             complement(2373..2807)
                     /gene="arfB"
                     /locus_tag="PLT69_05790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOJ13985.1"
                     /translation="MEGTRGDVTIDASELRWEFCRAGGPGGQNVNKVSSAVVLRFDVA
                     GSPSLAPGVKERLARLAGRRMTADGVLVIRAQRFRSQEKNREDALARLKDLILEAERV
                     PRRRVRTGPPASSRMARLEEKRSRARKKRMRAAARDHDPGGA"
     gene            complement(2858..3616)
                     /locus_tag="PLT69_05795"
     CDS             complement(2858..3616)
                     /locus_tag="PLT69_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOJ13986.1"
                     /translation="MLNQRREDGIIVATFEHGKTNSITMEVLRGIRDAVRQANTEEDV
                     KGIVLTGSGRVFCSGFDLPMFLGFKNVDEIIGFFHEEEEILLDLFTCAKPVVAAMNGA
                     AVAGGLIFAMAADYRIVKNHPKITIGMSEIRIGLPLSLAQSGVMRFGYDSDRKFRDLM
                     YFGRMMNVNEALEMGIVDEIVEEDQLIPRAKAVISAWIDNPGRAFIKLKEGLKKPVAD
                     RIRERLRTENWQDTLNCFFRKDVRDALEFVRAMM"
     gene            complement(3775..>4276)
                     /locus_tag="PLT69_05800"
     CDS             complement(3775..>4276)
                     /locus_tag="PLT69_05800"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ13987.1"
                     /translation="YALHSDEEALAEAARTQAMLTHTDALTVAASEFFARAAHMVLRG
                     AGPREAMEFLAGGRFSGTALAEAVAKGMATRGEDTIETVKGFGQDCHSPHALPSVAHI
                     VASYQDDLEEALVQNVMAGGDSAARGMIVGMLLGAHLGMQAIPRRWIDGLVRGPAVMG
                     LLGRVG"
BASE COUNT          732 a         1530 c         1248 g          756 t
ORIGIN      
        1 ctggacaggc tcgtgttctc caagatcagg gaacgcttcg gcgggaggct caggggcgcc
       61 atgaccgcca gcgcggccat gaaccccgag atcgccgggt tcttcttcga catcggcatc
      121 cccacctacg actgttacgg catgaccgag acctcgcccg cggtcacgat gaactgcccg
      181 tccgcctaca aactgggaag cgtcggcaga cccatcgaca aggtgaaggt cgtcatcgat
      241 tcctcggtcg tggaggaagg cgccgaggac ggggagatcg tggtgtacgg gcccaacgtc
      301 atgaagggct accacaacaa gcccgaggcc acggcagcgg tcatgacgcc tgacggcggc
      361 atccggaccg gcgacagggg caggttcgac aaggacgggt tcctctacat aacgggccgc
      421 atcaaggaac agtacaagct ggagaacggc aagttcgtct tcccggccgc cctcgaggag
      481 gacatccgcc tcatcccgta catagagaac gccatggtct acggcgagaa caagccgtac
      541 aacatctgca tcgtgatccc cgacttcgcg gtgctcggca agtacgcccg cgaaaacggg
      601 ctcccgaccg aacccgaaga gctcgtgaga cgcaaggagg tctacgacct catcgcggac
      661 gaggtgaaca aggccctcaa gggaaagtac ggcggttacg aaataccgaa gaagttcctg
      721 atcctgagcc agaacttctc ggtcgacgac ggcaccctga cccagaccct caaggtcaag
      781 cgccgcgtgg tgatccagag gctcaaggac tcgatcgagg cgctctacga gaaaaagtga
      841 cggccggagg cccctctcgg gttacacgga cacgctcgag cgagacccgt cacggcggcc
      901 cctttcgcct gcgccgccgg ctatcgtccc agggcggtgc atctccaccc ccccggccgc
      961 ggccgggcag aaccaccgcc cacaaccggc ggcgcgcacg cacacaggcc ttaagcccct
     1021 gccttcgccc ggccggcaga tgaccatccc catgcgccgt tcaaccccga gggtctcgag
     1081 ggtgggtagg gtcgtcgcgc cctctcgtgt tcgaccctgc tctcgagaca tgcctgacgg
     1141 tacgtcctag tccttcccga ggtacttgaa cgagaggctc aacttcgtgc ggtagttgac
     1201 gaccttgcca tcctcgatct gaaggtcgag ttccaccacc tccgcgatcc tcaggtcctt
     1261 gatggtcttg gacgccgtcg ccacggcggc ctccgcggcc ttctcccagg aaacggggct
     1321 ggtccctatg atctcgatca ccttgtagac gctctcagcc atgacaccct cctttcacga
     1381 accgtccagc cggtacgacc cttcgttagc gcaggcgccc ataaagaccc ggcggcggcc
     1441 gtctcaccac cgggccccct tctggtgata cttgggggtc aaggagaaga tcatgtgata
     1501 cctgaaggaa accctcagcc tcgcnnnnnn nnnnaagagc ggcctgcctt cttcgtctgc
     1561 gtgcatgtcc agcctcacca cctccgcgat ccgcaggtcc gtgagggtct cggacgcctc
     1621 tgcgacagcc gtctcagccg ctttctccca tgaaaccggg ctcgtgccca caagctcgat
     1681 cacctggtat tctcgctcag ccatacatgg ctcctttcgg tctgccacgc cagggcgcga
     1741 aagcgccccg ccctcctccc acaacgccgc cgtctccctc gaagacctcc cggaggatgc
     1801 cgtttccccg gcaacgggcc cgtcccgacc gtcctcctcg agtgcgccgc ctcctctgtc
     1861 attctgaata ataaaggcct tcctcgcaga ggcaatatgc gaaacgtttt ctgaatgaaa
     1921 ttgaccagga tttcaggtgt cgatcgcgtt tttggccgga ccgctcatga ccggcccggg
     1981 aggggacatc ggatgggtgt cgccagtccc tcaatgtatc cggaagcccg atcgtgcccg
     2041 gccggtgcaa acccttgtcc ggggcaccag gaaacccgcc tgcccctcga acaccaagga
     2101 acgcccgcgt cgcagcatca ccccccaggg gatcaccctc ggcggacatc ccgctgccga
     2161 cgatgcgtgg gtgtacctgg agcgcgggca tgtccttcct ggggcctggg cgctgccgga
     2221 ccggccgagc ccctacatgc ccgcaggccg ctcgagctgg acgcgcacgc tcgatgggac
     2281 ccaagggccc gggccggacg gaagggggga tctgcgggga gcgaacagcc gcgcccccgt
     2341 gcgggtacgc acgatggagg ccgttctccc cctcacgcgc ccccggggtc gtgatccctc
     2401 gccgccgccc tcatcctctt ctttcgagcc ctcgagcgct tctcctcgag cctcgccatg
     2461 cgtgaggagg cgggcggccc ggtcctcacc ctgcgcctcg gcacgcgctc agcctcgagg
     2521 atgaggtcct tgagccgagc gagcgcgtcc tcgcggttct tctcctggga cctgaagcgc
     2581 tgggccctga tgacgagcac gccgtccgcg gtcatgcgcc ttcccgccag gcgcgccagc
     2641 ctctccttca cgccgggggc cagggacggg gagcctgcga cgtcgaacct caacaccacg
     2701 gcgctcgaga ccttgttcac gttctgcccg cccggcccgc ctgcgcggca gaactcccag
     2761 cgcagttccg atgcatcgat cgtcacgtcg cccctggtac cctccatgac cgaaggtctt
     2821 gcctcccccg gaccgagccg tgcccgccgc ttgtgaccta catcatggcc ctgacgaact
     2881 ccagggcgtc ccgcacgtcc ttcctgaaga agcagttcag cgtgtcctgc cagttctccg
     2941 tcctcaggcg ctccctgatc ctgtctgcga cgggtttctt cagcccttcc ttgagcttga
     3001 tgaaggcccg gccagggttg tcgatccacg cggagatcac ggccttggcc ctggggatga
     3061 gctggtcctc ttccactatc tcgtcgacga tgcccatctc gagcgcctcg ttcacgttca
     3121 tcatcctgcc gaagtacatg aggtcgcgga acttcctgtc gctgtcgtag ccgaacctca
     3181 tgacgccgga ctgggccagt gacaggggca gcccgatcct tatctcggac atgcctatgg
     3241 tgatcttggg gtggttcttc acgatccggt aatccgcggc catggcgaat atgaggccgc
     3301 cggccactgc agccccgttc atggcggcga ccacgggctt ggcgcacgtg aacaggtcga
     3361 ggaggatctc ctcttcctcg tggaaaaacc cgatgatctc gtccacgttc ttgaaaccga
     3421 ggaacatcgg gaggtcgaac ccgctgcaga agaccctgcc cgagccggtg agcacgatgc
     3481 ccttgacgtc ctcttccgtg ttggcctgtc tgactgcatc ccgtatgccc cggagcacct
     3541 ccatggtgat ggagttcgtc ttgccgtgct cgaacgtcgc cacgatgatc ccgtcttccc
     3601 ttctctggtt cagcatgtca ccctcctcgt gggaacttga aatcaaacca tgcctataac
     3661 tcattccccg aaccttgtca tggttcaatc gtcgcgttcg ggcacgcgcc gcgccgtggc
     3721 gccagcgtgg ctgcagccgc ccctcgggcg cccccgggaa ccgtcacgcc gccatcaccc
     3781 tacccggccc aggagcccca tgacggcagg gccccttacg agcccgtcga tccaccgccg
     3841 ggggatcgcc tgcatcccca ggtgtgcgcc caggagcatg cccacgatca tccccctcgc
     3901 cgccgagtca ccgccggcca tgacgttctg cacgagcgcc tcctcgagat cgtcctggta
     3961 cgaggccacg atgtgcgcca cggacggcag ggcgtgcggg ctgtggcaat cctgcccgaa
     4021 cccctttacc gtctcgatgg tgtcttcacc cctcgtcgcc atgcccttcg cgaccgcctc
     4081 tgccagggcg gtaccggaaa acctgccacc tgccaggaac tccatggcct cacggggtcc
     4141 cgcccccctg aggaccatgt gggcagccct cgcgaagaac tccgaggcgg ccacggtgag
     4201 ggcgtccgtg tgcgtgagca tggcctgtgt ccttgccgcc tcggccaacg cctcctcgtc
     4261 gctgtggagc gcgtag
//
LOCUS       DAOSCR010000184         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13020_length_4272_cov_3.282903, whole genome shotgun sequence.
ACCESSION   DAOSCR010000184 DAOSCR010000000
VERSION     DAOSCR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13020_length_4272_cov_3.282903"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLT69_05805"
     CDS             complement(<1..603)
                     /locus_tag="PLT69_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ13988.1"
                     /translation="MASQWVDLRDLKFILFEVFKMGEALLGKGQFADHDVDMVNMVLD
                     QAAKFTENDIAPTYPDEVQRKPVEAVYKDGKVYVPEAYHRLYQLYCEGGWMTVSDSPE
                     VGGQGFPAVVAAACAQMFLAGNQAFLMYPGLTHGAARLVEDHFQHPLRDIILEKMYTG
                     VWAGTMCLTEPGAGSDVGALKTTAKKNPDGTYSITGTKCFI"
     gene            843..1679
                     /locus_tag="PLT69_05810"
     CDS             843..1679
                     /locus_tag="PLT69_05810"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOJ13989.1"
                     /translation="MQTVTGSFVGSGGLSLFWKGWLPQGDVKAVIHLIHGYAEHVDRY
                     ANVVNELVPAGYAVMGNDHRGHGRSDGRRGHVRRFQDFVDDEHLFYLQVIRFRMPDVP
                     RFVLGHSMGSLIAMDYVEQRPENVKGLVLSGTGSKPGPDIPPLLVKITGILSSVLPWV
                     HVKSPLPPEFISRDEDVVKAYVEDPLVYNVITPRLAHEMNKSVVRVASGAHALAMPVL
                     IQLGSLDTSFSGQRELFEAIGSKDKTFKLYEGLKHEVYNELPQDRKRVLEDLRAWLDA
                     HL"
     gene            complement(1841..3289)
                     /gene="ahcY"
                     /locus_tag="PLT69_05815"
     CDS             complement(1841..3289)
                     /gene="ahcY"
                     /locus_tag="PLT69_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOJ13990.1"
                     /translation="MAEKAKKLSCSTLPDYCGLPCKIADRSLAEFGRKEIAIAEHEMP
                     GLMAVREKYAPAKPLEGARITGSLHMTIQTAVLIETLKELGADVRWASCNIFSTQDHA
                     AAAIASGGTPVFAWKGESLEDYWACTLAALTFPGGKGPNLIVDDGGDATLMVHRGYYA
                     EENPAILDEPTENKELAIINEILKKRLAEDPTFWHRTVKELRGVSEETTTGVHRLYQM
                     LEKKTLLFPAVNVNDSVTKSKFDNLYGCRESLADGIKRATDVMVAGKVVCVCGYGDVG
                     KGCAQSMRGFGARVLVTEIDPICALQAAMEGYQVTTVEDALQEANIFVTATGNCRVIR
                     IEHMERMLDQSIVCNIGHFDDEIEVDKLNAYPGITRVNIKPQVDKYIFPDGHCIYLLA
                     EGRLVNLGCATGHPSFVMSNSFTNQVLAQMDLWKNPREVSVRTLPKILDEEVARLHLT
                     KLGAKLTTLTQEQADYIGVSVDGPFKPEHYRY"
     gene            complement(3292..>4272)
                     /gene="metK"
                     /locus_tag="PLT69_05820"
     CDS             complement(3292..>4272)
                     /gene="metK"
                     /locus_tag="PLT69_05820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ13991.1"
                     /translation="HQFDVQRIARQTIKEIGYTEAVMGFSWNTCGVMVSIDKQSPDIN
                     QGVQEGQGLFAEQGAGDQGMMFGFACDETDMLMPTPIYYAHKLTKALADTRKSGVLEF
                     LRPDGKSQVSVQYEDGRPARIDGIVIAAQHDEDASYETVREGIMEEVVKKQIPAHLLH
                     KDTKYFINATGRFVLGGPHADTGLTGRKIIVDTYGGFASHGGGAFSGKDPTKVDRSAA
                     YMARKIAKNIVASGLARRCTVQLAYAIGVADPVSIMVDTQGTGKISDDRLAQIVREVF
                     PLKPKGIIEHLDLLRPIYRKTAAYGHFGRPEPEFTWERTDMVEKLLERAG"
BASE COUNT          647 a         1364 c         1368 g          893 t
ORIGIN      
        1 gatgaagcac ttggtgcccg tgatggagta cgtgccgtcc gggttcttct tggccgtggt
       61 tttcagggcc cccacgtccg aaccggcgcc cggctcggtg aggcacatgg tgccggccca
      121 cacgcccgtg tacatcttct cgaggatgat gtcgcggagc gggtgctgga agtggtcttc
      181 cacgagtctg gctgcgccgt gggtgagacc gggatacatg aggaaggcct ggttgccggc
      241 gaggaacatc tgtgcgcagg ccgcggcgac caccgccggg aagccctggc ctcccacctc
      301 ggggctgtcg gacacggtca tccacccgcc ctcgcagtag agctggtaca gccggtggta
      361 cgcctcgggc acgtacacct tgccgtcctt gtacacggcc tcgacgggct tgcgctgaac
      421 ctcgtcgggg tacgttgggg cgatgtcgtt ctcggtgaac ttcgcggcct ggtcgagcac
      481 catgttcacc atgtcgacgt catggtcggc gaactggccc ttgccgagaa gggcctcccc
      541 catcttgaag acctcgaaca gtatgaactt gaggtccctc aggtccaccc attgactagc
      601 cataataccc tccttgcttg agtgtcgttt gcgtgtcgtc ttgcagcccc atgaatgagc
      661 actcattctt ataccccaca tgcggtgctt atgtcaacag aggttcttga ttttttcgcg
      721 gacctggtgc ataaagacat ctcgtttgtt tgcgtcgtcc tcgaaacgct tatctggcga
      781 gtccgggtct tgcgggcggg gtttacgata ccatggctgc attggaagga gggtcgtcgg
      841 gcatgcagac cgtcacgggg agcttcgtcg ggtcgggagg tctttccctc ttctggaagg
      901 gttggctgcc tcaaggggat gtcaaggcgg tcatccacct catccacggc tacgccgagc
      961 acgtggaccg ctatgccaac gtcgtgaacg agctggttcc cgcagggtac gcggtgatgg
     1021 gcaacgacca ccgcggtcac ggcaggagcg acggcaggag ggggcacgtg cgacgtttcc
     1081 aggatttcgt ggacgacgag cacctcttct acctccaagt gatcaggttc cgcatgccgg
     1141 atgtccccag gttcgtcctc ggtcactcca tggggagcct catagccatg gattacgtgg
     1201 agcagaggcc cgagaacgtg aagggtctcg tgctctcggg gaccggctcc aagcctgggc
     1261 cggacatccc gcccctgctc gtcaagatca ccgggatcct ctcctcggtg ctgccctggg
     1321 tgcacgtgaa gtcgccgctc ccgccggaat tcatctcgcg ggatgaggat gtggtgaagg
     1381 cctacgtgga ggaccccctg gtgtacaacg tcatcacgcc gaggctcgcg cacgagatga
     1441 acaagtccgt ggtcagggtg gcctcagggg cgcatgcgct cgccatgccc gttctcatcc
     1501 agctgggctc gctcgacacc tcgttctcgg gccagcggga actcttcgag gcgatcggtt
     1561 cgaaggacaa gaccttcaag ctctacgagg ggctcaagca cgaggtctac aacgagctgc
     1621 ctcaggacag gaagagggtg cttgaagacc tgcgcgcgtg gctggatgcc cacctctgac
     1681 ccaggcgcct ttccttcgcg gtcgctcgag gacacggcct gccgtgtaca ctgatgcctg
     1741 tcccctctcg tgacgcctgt cccttcgtga cctcgcggga ccgaaccctg ccttgatgcc
     1801 tcgccgccag gtgcgggtgt gagatcgagc accccgcggg tcagtagcgg tagtgttcgg
     1861 gcttgaaggg cccgtccacg ctcaccccga tgtagtccgc ctgttcttgg gtcagcgtgg
     1921 tcagcttggc gccgagcttg gtgaggtgga gcctcgcgac ctcctcgtcc aggatcttgg
     1981 gcagcgtcct cacgctcacc tcgcgggggt tcttccagag gtccatctgc gccagcacct
     2041 ggttcgtgaa cgagttggac atgacgaagg acgggtgccc cgtggcgcac ccgaggttca
     2101 cgagcctccc ctcggccagc aggtagatgc agtgcccgtc cgggaagatg tacttgtcca
     2161 cctggggctt gatgttgacc cgggtgatcc ccgggtaggc gttcagcttg tcgacctcga
     2221 tctcgtcgtc gaagtggccg atgttgcaca cgatcgactg gtccagcatc cgctccatgt
     2281 gctcgatgcg tatgacgcgg cagtttccgg tggccgtgac gaagatgttg gcctcttgga
     2341 gggcgtcctc cacggtggtg acctggtagc cttccatcgc ggcctggagg gcgcatatgg
     2401 ggtctatctc ggtgacgagg acccgcgccc cgaagcccct catggactgg gcgcagccct
     2461 tgcccacatc gccgtagccg cacacgcaga ccaccttgcc cgccaccatc acgtcggtgg
     2521 ccctcttgat gccgtccgcc agggattccc tgcacccgta gaggttgtcg aacttgctct
     2581 tggtgaccga gtcgttcacg ttgacggctg gaaacagcag cgtcttcttc tcgagcatct
     2641 ggtagagcct gtggacgccg gtggtcgtct cctcggagac ccccctgagc tccttgacgg
     2701 tcctgtgcca gaaggtgggg tcctccgcga ggcgcttttt gaggatctcg ttgatgatcg
     2761 cgagttcctt gttttccgtg ggctcgtcga ggatcgcggg gttctcctcc gcgtagtacc
     2821 cccggtggac catgagggtg gcgtccccgc cgtcgtccac gatcaggttc ggccccttcc
     2881 cgcccgggaa ggtgagcgcc gcgagcgtgc atgcccagta atcctcgagg ctctcgccct
     2941 tccacgcgaa gaccggcgtc ccgcccgatg cgatggccgc cgccgcgtga tcctgggtgg
     3001 agaagatgtt gcacgacgcc catcgcacgt cggcccccag ctccttgagg gtctcgatga
     3061 ggaccgcggt ctggatggtc atgtgcaggg aaccggttat gcgggcgccc tcgagcggct
     3121 tggccggcgc gtacttctcg cgcaccgcca tgaggccggg catctcgtgc tccgctatcg
     3181 cgatctcctt gcggccgaac tcggccaggg accggtccgc gatcttgcac ggcaggccgc
     3241 agtagtccgg gagggtcgaa caggacagct tcttcgcctt ctcagccata ctcatcccgc
     3301 cctttcgagg agtttttcca ccatgtcggt gcgttcccac gtgaactcgg gttcgggtct
     3361 cccgaagtgc ccgtacgccg cggtcttcct gtagatcggc ctcaggaggt cgaggtgctc
     3421 gatgataccc ttcggcttga gcgggaagac ctccctgacg atctgcgcca gcctgtcgtc
     3481 ggatatcttc ccggtcccct gggtgtccac catgatggac acgggatcgg cgacgccgat
     3541 ggcgtaggcg agctgcacgg tgcagcgtct ggcgagaccc gaagccacga tgttcttggc
     3601 tatcttgcgc gccatgtacg cggcggagcg gtccaccttg gtggggtcct tgcccgagaa
     3661 ggccccgccc ccgtggctgg cgaaaccgcc gtaggtgtca acgatgatct tcctgccggt
     3721 caggccggtg tccgcgtgcg gcccgcccag gacgaacctg ccggtggcgt tgatgaagta
     3781 cttcgtgtcc ttgtggagga ggtgggcagg gatctgtttc ttgaccacct cctccatgat
     3841 cccctcgcgc acggtctcgt agctcgcgtc ctcgtcgtgc tgcgccgcga tcacgatgcc
     3901 gtcgatcctc gccggcctgc cgtcctcgta ctgtacggat acctgcgact tcccgtcggg
     3961 cctgaggaac tcgagcacgc cgctcttgcg cgtgtccgcg agcgccttgg tgagcttgtg
     4021 ggcgtagtag atgggggtgg gcatgagcat gtctgtctcg tcgcaggcga acccgaacat
     4081 catgccctgg tccccggccc cctgctcggc gaagagcccc tgtccctcct ggacaccctg
     4141 gttgatgtcc ggggactgct tgtcgatgct caccatcacg ccgcaggtgt tccagctgaa
     4201 ccccatgacg gcctccgtgt agccgatctc cttgatggtc tgccgtgcga tgcgctggac
     4261 gtcgaactgg tg
//
LOCUS       DAOSCR010000185         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13171_length_4236_cov_3.594595, whole genome shotgun sequence.
ACCESSION   DAOSCR010000185 DAOSCR010000000
VERSION     DAOSCR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13171_length_4236_cov_3.594595"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PLT69_05825"
     CDS             <1..1354
                     /locus_tag="PLT69_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOJ13992.1"
                     /translation="ERATVPIDIRIPKGHDCLVITGPNAGGKTVALKTLGLLVLMAKS
                     GLAVPAREGSTIAPFGAVHVEMDTSQDITHDLSSFTAHALTLKGIYGKTQRGDLVLLD
                     EPGSGTDHEQGSALAVACIHALREKGATVVVTSHADLVKLYGVTSKGVQNAATAFDET
                     GLKPLYTLQYGMVGQSRAFEILESIDFPRELIEQARGIVNRDTSSTLARAVMDMTQAH
                     VMREEASRELAEARRMREEAARELEAHRKERLQSALRYKNLLDRLEKMAKRKAAPEEV
                     RRVREDPETAEVERVIEEAGAPAEALDVSPGARVRLKGSDTEGVVVGVDQDSAEVSLA
                     GKRVRVGLDQVTAVPGAGSVGGAGRTVSRMQAPPRVLPIKVEGLRVDEALPVVERAVD
                     RAVLTGQRQLEIIHGAGTGRLKNAIRAYLRELPCVKAVEDAPMSEGGGNKTIVTLEGE
                     "
     gene            1357..3096
                     /gene="dnaG"
                     /locus_tag="PLT69_05830"
     CDS             1357..3096
                     /gene="dnaG"
                     /locus_tag="PLT69_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOJ13993.1"
                     /translation="MDRSRLERIKQALDIVSVVAEHVSLERKGGRLWGLCPFHSEKTP
                     SFAVDAARQTFKCFGCGKGGDVFTFLMEKKGLSFAEAAEELAKRAGIEPEERPASSRR
                     SGNKALYEANACAAEFFVETLSSPEGEPARRYLRGRGIDEATQSRFRIGYAPESWDAL
                     LRRLRARGVPPTVAERAGLVVSKKGGGYRDRFRGRVMFPIVDLSGEVAGFGGRIIGPG
                     EPKYLNTSESPIFEKRRLVYNLHSARSAIRKGGAVVVEGYMDVVSLAQAGFDAAVATL
                     GTALGEDHVRLLMRFTDDITLVFDGDESGRKAMVRAIEPFAASGVIPRVVILPQGKDP
                     DDIAREGIEAFRSILETARDIWEFVFDESFSRNDPSKLRGQNAILKELTPMIAHARDP
                     VLRDLLSHRLASRLGVSPDVVARMCEVRSADTAGEPGQGYGAERKGIETTLARLMVLD
                     RAAASKVREKAPALEFSDAELQGLFDYMVKGGDDPLADPSCPDRVRLTASRLAAQGEF
                     PGDAKKALIDTLFRFKSLAIDERLKRIQHELLAAERARDTARRNELLKEKQEAMIERR
                     GLRQRIMEELEDR"
     gene            3093..>4236
                     /locus_tag="PLT69_05835"
     CDS             3093..>4236
                     /locus_tag="PLT69_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOJ13994.1"
                     /translation="MSDKKQYPKEIQRLIAMGKSKGFLTFDEVNEQLPDDILNPDDLD
                     DVMDLFTEKEIQLVDTEEDFAPDDEEFDFHDDGSMRSPDPVKMYLHEMGSVSLLSRED
                     ETEIAKRIEAGQRQILNVIINSPVTVKEVIQIGNAIACGEKRLKDVVDIDDIVDVDEE
                     AVRAKTLAIIKEIEEKQGELKDLKYQLFKAKSKSQKEKIKSNMAKVSREIVLLIHDIH
                     FLPQQIEQFGNRLKDLKRVLEEREATVNRCQERFNKTVDEIRQMHKTLCNGDCDKAAE
                     RFLKDHGLSREAFVETAGEMIDALKDIKRIEMEGGLTAHGLKKAVSILEDAEQKTNAA
                     KTELIQANLRLVVSIAKKYVNRGLQFLDLIQEGNIGLMKAVEKFEYR"
BASE COUNT          880 a         1231 c         1524 g          601 t
ORIGIN      
        1 cgagcgggcc acggtcccga tcgacatccg catccccaag gggcatgact gcctggtcat
       61 cacgggcccc aacgcaggcg gcaagaccgt ggcgctcaag acgttggggc tcctggtgct
      121 catggccaag agcgggctcg ccgtgcccgc gcgggaaggc tccacgatcg cccccttcgg
      181 ggcggtgcac gtggagatgg acacgagcca ggacatcacc cacgacctct cctccttcac
      241 ggcgcatgcg ctgaccttga aggggatcta cgggaagacc caaaggggtg acctcgtgct
      301 cctggacgag ccgggttcgg gcacggacca cgagcaggga tccgcgctcg cggtggcctg
      361 catacacgcc ctcagggaaa agggcgcgac cgtcgtggtg acctcgcacg ccgacctcgt
      421 gaagctctac ggtgtgacgt ccaagggggt gcagaacgcg gcgaccgcgt tcgacgagac
      481 cggcttgaag cccctgtaca cgctccagta cggcatggtc ggccagagca gggccttcga
      541 gatcctggag tccatcgact ttcccaggga actcatcgag caggcccggg gcatcgtgaa
      601 ccgggacacc tcctccacgc tcgcccgtgc ggtcatggac atgacccagg cgcatgtcat
      661 gcgggaagaa gccagccgcg agctggccga ggcgagaagg atgcgggagg aggcggcaag
      721 ggagctcgag gcgcacagga aggagaggct gcagagcgcc ttgcgctaca agaacctcct
      781 ggacaggctc gagaagatgg cgaaaaggaa ggccgccccc gaggaggtca ggagggttcg
      841 agaagaccct gagacggccg aggtggaacg ggtcatcgag gaggccgggg cgccggccga
      901 ggcgctggat gtgtcaccgg gcgccagggt gcgcctgaag ggctcggaca ccgaaggcgt
      961 ggtcgtgggc gtggaccagg acagcgccga ggtgtccctt gccggcaaga gggtgagggt
     1021 ggggctggac caggtcacgg ccgtgcccgg cgcagggtcc gtcggcggcg ccggccgcac
     1081 ggtctcgagg atgcaggcac cgccacgtgt gctgcccatc aaggtggagg ggctcagggt
     1141 ggacgaggcg ctgccggtgg tggagcgggc cgtggacagg gccgtgctca cggggcagag
     1201 gcagctggag atcatccacg gcgcaggcac cgggaggctc aagaacgcca tacgggccta
     1261 cctcagggaa ctgccgtgcg tcaaggcggt ggaggacgcg ccgatgtccg agggcggggg
     1321 aaacaagacc atcgtgacgc tcgaggggga gtgagggtgg acaggtcgcg cctcgagagg
     1381 atcaagcagg ccctcgacat cgtctccgtc gtcgcggagc acgtgagcct cgagaggaag
     1441 ggggggaggc tctggggact ctgccccttc cacagcgaga agacgccgtc gttcgccgtg
     1501 gatgcggcca ggcagacctt caagtgcttc gggtgcggca agggcgggga cgtgttcacg
     1561 ttcctcatgg agaagaaggg cctgtcgttc gccgaggcgg ctgaagagct ggccaagagg
     1621 gcgggcatcg agcccgagga gcggcccgcg tcctcgaggc ggtccgggaa caaggccctc
     1681 tacgaggcga acgcctgcgc agcggagttc ttcgtggaga cgctctcctc gcccgagggc
     1741 gagcccgcgc gcaggtatct caggggcagg gggatcgacg aggccacgca gtcacgcttt
     1801 cgcatagggt atgcgccgga gtcctgggat gcgctcttga ggcgcctgag ggcgaggggc
     1861 gtgcccccca cggtggcgga gagggccggg ctcgtggtct cgaagaaggg cggcggctac
     1921 cgggacaggt tcagggggcg cgtgatgttc cccatcgtgg acctctcggg cgaggtggcc
     1981 ggcttcggtg gcaggatcat cggcccggga gaacccaagt acctcaacac ctccgaatcc
     2041 cccatattcg agaagaggag gctcgtctac aacctgcact cggcgaggag cgccatcagg
     2101 aaaggcggcg ccgtggttgt cgaggggtac atggacgtcg tctccctcgc ccaggcgggt
     2161 ttcgatgccg ccgtcgcgac gctgggcacg gccctgggag aggaccacgt gaggctcctc
     2221 atgcgcttca cggacgacat cacgctggtc ttcgacggcg acgagtccgg caggaaggcc
     2281 atggtcaggg cgattgagcc gtttgccgcc tcgggggtga tcccgagggt cgtgatcctg
     2341 ccccagggca aggacccgga cgacatcgcg cgggagggca tcgaggcgtt caggtccatc
     2401 ctcgagacgg ccagggacat atgggagttc gtcttcgatg aatccttttc caggaacgat
     2461 ccatctaaac tccgtggtca gaacgccatc ctgaaggaat tgacgccgat gattgcacat
     2521 gcaagggatc ccgtgctgag ggatctcctc tcccacaggc ttgcctccag gctcggggtg
     2581 tccccggacg tggtggcgag gatgtgcgag gtgcgctctg ccgatacggc gggggaaccc
     2641 ggccagggat acggtgcaga gaggaagggc atagagacga cgctcgcgag gctgatggtc
     2701 ctcgacaggg cagcggcatc gaaggtcagg gagaaggcgc ccgcgcttga gttctcggac
     2761 gccgagctcc aggggctctt cgactacatg gtgaaaggcg gggacgatcc cctcgcggac
     2821 ccgtcgtgcc cggacagggt gaggctcacc gcctcgcggc tggccgccca gggggagttc
     2881 cccggggacg cgaaaaaggc cttgatcgac accctcttca ggtttaagag tcttgcgatc
     2941 gacgagaggc tcaagaggat ccagcacgag ctccttgccg ctgagcgcgc ccgcgacacc
     3001 gcaaggcgca acgagctgct caaggagaag caggaggcga tgatcgagcg caggggtctg
     3061 cgccagcgaa tcatggagga actcgaggac agatgagcga caagaaacag tacccaaagg
     3121 agatacagag gttgatagcc atgggcaaat caaagggatt cctgaccttc gacgaggtga
     3181 acgagcagct gcccgacgac atcctgaacc ctgacgatct cgacgacgtc atggacctgt
     3241 tcacggagaa ggaaatccag ctcgtggaca ccgaggaaga cttcgcgccc gacgacgagg
     3301 agttcgactt ccacgacgac gggtccatgc gttcccctga cccggtcaag atgtacctcc
     3361 acgagatggg gtccgtgtcg ctgctctccc gcgaggacga gaccgagatc gccaagcgga
     3421 tcgaggcggg ccagcgccag atcctcaacg tgatcatcaa ctcgccggtc acggtcaagg
     3481 aggtcatcca gatcggcaac gcgatcgcgt gcggggaaaa gcgtctcaag gacgtggtgg
     3541 acattgacga catcgtggac gtcgacgagg aggcggtcag ggccaagacc ctcgccatca
     3601 tcaaggagat agaggagaag cagggggagc tcaaggacct caagtaccag ctcttcaagg
     3661 cgaagagcaa gtcgcagaaa gagaagatca agtcgaacat ggcaaaggtc agccgggaga
     3721 tcgtgttgct catccacgac atccacttcc tcccccagca gatcgagcag ttcgggaaca
     3781 ggctcaagga tctcaagagg gtgctggagg aacgcgaggc cacggtgaac cggtgccagg
     3841 agcgcttcaa caagacggtg gacgagataa ggcagatgca caagacgctg tgcaacggcg
     3901 actgcgacaa ggccgcggaa aggtttctca aggatcacgg gttgagcagg gaggccttcg
     3961 tggagacggc cggcgagatg atagacgccc tcaaggacat caagcgcatc gagatggagg
     4021 gcgggctcac ggcgcacggg ctgaagaagg cggtgagcat cctcgaggac gccgagcaga
     4081 agaccaacgc cgccaagacc gagctcatcc aggcgaacct gaggctcgtg gtcagcatcg
     4141 ccaagaagta cgtgaaccga ggtctccagt tcctggacct catccaggag ggcaacatcg
     4201 gcctcatgaa ggcggtggag aagttcgagt acagga
//
LOCUS       DAOSCR010000186         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13345_length_4194_cov_3.441894, whole genome shotgun sequence.
ACCESSION   DAOSCR010000186 DAOSCR010000000
VERSION     DAOSCR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13345_length_4194_cov_3.441894"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLT69_05840"
     CDS             <1..224
                     /locus_tag="PLT69_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOJ13995.1"
                     /translation="RIRITARGRLRPCLFSEEELDIMTPLRSGVTDEELEAVIVEGVR
                     RKPASHGACRGTSIGDRGPLRQAMHTIGG"
     gene            complement(228..584)
                     /locus_tag="PLT69_05845"
     CDS             complement(228..584)
                     /locus_tag="PLT69_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13996.1"
                     /translation="MVKTKDKRVRERISLHDVRGTVEIDDTLKPVTVLNASEEGVCIA
                     GAAIETGTVVRLSIEDTLGLGAFSLYCRSAWSSSKKKPDKLTGLFLLNTNKVLFTKDL
                     ATFNRLIDTVRRQRNP"
     gene            916..2268
                     /locus_tag="PLT69_05850"
     CDS             916..2268
                     /locus_tag="PLT69_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ13997.1"
                     /translation="MRGRKVLLVNPWIHDFAAYDLWARPLGLLIVGSVVRRNGVEVSF
                     IDCLGDDLASDRRPGGHGKFRRSTIEKPEPLGFVPRTYARYGIDPGEFARRLEEIPAP
                     DAVLVTSMMTYWYPGVREAVGIVKDAFPGVPVILGGVYATLCTDHARLASGADYVVAG
                     TGEARVIELLCSLWGCSAEYVPDEDDLDSLPYPAFDVMDNLDSVCVRTARGCPYRCAY
                     CAAPVLSGSPSYRKTRAVIEEILHWERSRGVKDVAFVDDALLTPRPRALEFLGGLASK
                     SGDVRFHCPNALHAREISSEVARLMREAGFVTVRLGLETADAFRQRATGGKVTNDEYL
                     WAVDALVGAGYESRDIGVYVLCGLPDQEPEEVLDTVRFVLSSGATPLLAEYSPIPHTP
                     LWDEAQKASPYPISDEPLFHNNTILPCAQRRFLEVFPEIKRLALTGRRASWTPCARDP
                     "
     gene            complement(2222..3181)
                     /locus_tag="PLT69_05855"
     CDS             complement(2222..3181)
                     /locus_tag="PLT69_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOJ13998.1"
                     /translation="MKYLVMGAGALGSVFGGMLAEHGHDVTFVGADDHLLAMREQGLT
                     ITGIWGEHHVHPVKAHYKTEGLEGVFDVVLLCVKSYHTATVIAQTAPYLHESSLVFSI
                     QNGLGNWEIIADAVGWDRTVGARVIFGAEVPRPGTARVTVYADKVLLGSPSGTAPKDK
                     IDTVRSHLDSAGIPCSVTDDIESSLWGKVLYNCSLNALGAILNVPYGELSETPALMDM
                     IRRVIGEIFEVARAKGVKLPYAHAGEYFRVLTEVQLPPTASHRSSMLQDLSRGSLLTE
                     IDALNGAISRYGRELGVMTPANDFLTAIVKGLEHRASTRHASR"
     gene            complement(3178..>4194)
                     /locus_tag="PLT69_05860"
     CDS             complement(3178..>4194)
                     /locus_tag="PLT69_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ13999.1"
                     /translation="MGDVAFLGQSGGMTHNFMRMATARYMELNKVVSYGNQIDLGVDD
                     YLEYLAGDETVKVIACYIEDIKRGRRFAEVLGRAVSKKPVIVLKGGVTEQGAKAAYSH
                     TGAMAARHELWTALMHQRRAIEAETFEHLMDLAMVAVARRRPEGPRLGFLGAGGGTSV
                     LFTDLSSRAGLALPQLHTATQRRIASRISSVNTSTANPVDLGFYGFDFTIMAHTIEAM
                     ASDPSIDVIVPYFSLDFITSFQKDQIETGPHAIVQAASSIDKPVVPILSQFTENSIDI
                     EEVRIRMTAIFRKAGLAVYRTPQDAVAAIQGYLTWRRGLSPAGETLHRDAVPLREERE
                     AAKA"
BASE COUNT          710 a         1312 c         1404 g          768 t
ORIGIN      
        1 accgtatccg tatcaccgcg agggggcggc tgaggccgtg cctcttttcc gaagaagagc
       61 tcgatatcat gacccccctg aggagcggtg tcacggacga ggagctcgaa gccgtgatcg
      121 tggagggcgt gaggcggaag cccgcttcgc acggtgcatg caggggaacg agtatcggcg
      181 acagaggccc gctccgccag gcgatgcaca cgataggcgg gtgaccgtca ggggttgcgc
      241 tggcgccgaa cggtgtctat gagcctgttg aacgtggcga ggtccttcgt gaagaggacc
      301 ttgttggtgt tcagcaggaa gaggcccgtg agcttgtcgg gtttcttctt cgaggatgac
      361 catgcggacc tgcagtacag ggaaaaggcc cccagcccga gggtgtcctc tatggacagc
      421 cggacgaccg ttcccgtctc tatggccgca cccgcgatgc acacgccttc ctcgctggcg
      481 ttgaggaccg tcaccggctt gagggtgtcg tcgatctcca cggtgcccct tacatcgtgc
      541 agggaaatgc gttcgcgaac ccgcttgtct ttcgtcttca ccatggtcgt caaacatgta
      601 catgcacaaa gaatgcctgt caaggaggtc tctcctgcct gagacgtgtc ttgcacacgc
      661 catgacccgg gatgggcggg cctccgccga gggacgcctt gttgccgggg gcagaagggg
      721 catcggcgcc gtgccctcgg ggagcatgat cggcggggct agaagcacac gatgcacgtt
      781 ctggttccca gcgggaggtc gcgacgttcg agatcgaggc ctcggcttgc aacagggccc
      841 gagaaggtgc gggtttcatg agagggtggt cgtccctctc tgcgagtccg ccgcgaggga
      901 ggagggatcc tgcaggtgag ggggcgcaag gttctcctcg taaacccgtg gatccacgac
      961 ttcgccgcct acgacctgtg ggcgaggccg ctggggttgc ttatcgtggg ctcggtggtg
     1021 cggagaaacg gggtcgaggt gagtttcatt gactgtctcg gcgacgacct cgcttcggac
     1081 cggaggccgg gcggccacgg caagttccgc cgaagcacga tcgagaagcc cgaacccctc
     1141 gggttcgtac cgaggacgta cgcgcgctac ggcatcgatc ccggggagtt cgcgcgcagg
     1201 ctcgaggaga tccccgcgcc tgacgccgtg ctcgtgacat cgatgatgac gtactggtac
     1261 cccggcgtga gggaggccgt cggtatcgtc aaggatgcct tccccggtgt ccccgtgatc
     1321 ctgggcggcg tctacgcgac cctctgcacc gaccacgccc gcctcgcgag cggggcggac
     1381 tatgtcgtcg ccggaaccgg ggaagcgcgc gttatagaac tgctctgctc gctttggggg
     1441 tgttccgccg agtatgtccc ggacgaggac gatctcgact ccctgcccta tcccgccttc
     1501 gatgtgatgg acaacctcga cagcgtgtgt gtcaggacgg ctcgagggtg cccgtaccgg
     1561 tgcgcctact gcgcggcgcc cgtcttgtcg ggaagcccgt cctacaggaa gacccgggcc
     1621 gtcatcgaag agatcctcca ctgggaaaga agccgcggcg taaaggatgt ggccttcgtg
     1681 gacgacgcgc tcctgacgcc gcgcccgcgt gccctggaat tcctcggggg ccttgcaagc
     1741 aaaagcggtg acgtgaggtt ccactgcccg aacgccttgc acgcccggga gatctcatcg
     1801 gaggtggccc ggctcatgcg cgaggccggg ttcgtgaccg tcaggctcgg cctcgagacg
     1861 gcggatgcct tcaggcagag ggcgacaggc ggcaaggtga ccaacgacga gtacctgtgg
     1921 gctgtagacg ccctagtagg tgcgggatac gaatcccgcg acataggggt gtacgtgttg
     1981 tgcggcctgc cggaccagga accggaagag gtcctcgaca ccgtaaggtt cgtgctctcg
     2041 tcgggtgcga cccccctgct cgccgaatac tctcccatcc cccacacccc gctgtgggac
     2101 gaggcgcaga aggcaagccc ctaccccatc tcggacgagc ccctgttcca caacaacacg
     2161 atccttccct gcgcgcaaag acggtttctg gaggtgttcc ccgagatcaa gcgcctcgcg
     2221 ctcaccggga ggcgtgcctc gtggacgccc tgtgctcgag acccttgacg atcgcggtca
     2281 ggaagtcgtt cgcaggggtc atgaccccga gttcccggcc gtagcggctg atcgcgccgt
     2341 tgagggcgtc gatctcggtc aggagggatc cccgggagag gtcctggagc atggacgagc
     2401 gatgagacgc ggtcgggggc agttggacct cggtgaggac cctgaaatac tcgcctgcat
     2461 gtgcataggg cagcttcacg ccctttgccc gggcgacctc gaagatctct cctatgacgc
     2521 ggcggatcat gtccatgagc gccggcgtct ccgagagctc cccgtacggc acgttgagga
     2581 tggcgcccag ggcgttgagc gaacagttgt agaggacctt gccccacaag gacgactcga
     2641 tgtcgtccgt cacggagcac gggatacccg cactgtcaag gtgggagcgg accgtgtcga
     2701 tcttgtcctt gggcgccgta cccgaaggcg atccgagaag gaccttgtcc gcgtacacgg
     2761 tgacccgcgc ggtgccgggc cggggcacct cggcgccgaa gatcacccgg gcgcccacgg
     2821 tgcgatccca ccccaccgca tcggcgatga tctcccagtt tcccaggccg ttctggatgg
     2881 agaacacgag gctcgattcg tggagatacg gcgccgtctg ggctatgacg gtcgcggtgt
     2941 ggtacgactt cacgcagagg agcacgacgt cgaagacacc ctcgaggcct tccgtcttgt
     3001 agtgggcctt caccgggtgg acgtggtgct caccccagat acccgtgatg gtgagaccct
     3061 gctccctcat ggcgaggaga tggtcgtcgg cgccgacgaa ggtcacgtca tgaccgtgct
     3121 cggccagcat gccgccgaag acgctgccga gcgcgccggc gcccatgacg aggtatttca
     3181 tgccttcgcg gcctcccttt cctctctgag cggcaccgcg tccctgtgga gggtttcccc
     3241 cgccggagaa aggccgcgcc gccacgtcag gtacccctgt atggctgcaa cggcgtcctg
     3301 gggcgtcctg tagacggcca ggccggcctt cctgaatatg gctgtcatgc gtatccgcac
     3361 ttcctctatg tcgatcgagt tttccgtgaa ctgggagagg atggggacga caggcttgtc
     3421 gatcgacgag gccgcctgca cgatggcatg gggccctgtc tctatctggt ccttctggaa
     3481 cgaggtgatg aagtcaaggg agaaatacgg gacgatcaca tcgatcgacg ggtctgaggc
     3541 catggcctcg atggtgtgcg ccatgatggt gaaatcgaag ccgtaaaatc ccaagtccac
     3601 ggggtttgcc gtgctcgtgt tcacggagct gatcctgctc gcaatcctcc tttgggtggc
     3661 ggtgtggagc tgggggagag cgagcccggc acgggaggag aggtccgtga acagcacgga
     3721 cgtccccccg ccggcgccga ggaacccgag cctcggtccc tcgggccgcc tcctcgccac
     3781 ggccaccatg gcaaggtcca tgaggtgctc gaaggtctcc gcctcgatcg ccctcctctg
     3841 gtgcatgagg gccgtccaga gttcgtgcct ggccgccatg gcccccgtgt gggaataggc
     3901 cgccttggcc ccctgctccg tcacgccgcc cttgagcacg atgacgggct ttttcgagac
     3961 ggccctgccg aggacctccg cgaaacgcct gccgcgcttg atgtcctcga tgtaacaggc
     4021 gatcaccttg accgtttcgt cgccggcgag gtactcgagg tagtcgtcca cgcccaggtc
     4081 tatctggttg ccgtaggata cgaccttgtt gagctccatg tagcgtgctg tcgccatcct
     4141 catgaaattg tgcgtcatcc cgccggactg gcccaagaac gcgacgtccc ccat
//
LOCUS       DAOSCR010000187         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13365_length_4190_cov_3.754534, whole genome shotgun sequence.
ACCESSION   DAOSCR010000187 DAOSCR010000000
VERSION     DAOSCR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13365_length_4190_cov_3.754534"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLT69_05865"
     CDS             complement(<1..363)
                     /locus_tag="PLT69_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ14000.1"
                     /translation="MPSHKTAPGKKTTHAYKPVSGWELLEGKNDRIEAYARDYTAFLS
                     SVKTEREAVSYIRQRASKDKNLVCMENRGRALALCRAGARPLEEGLRIVVAHLDSPRL
                     DLKAKPLYEDTEIAMLKTH"
     gene            complement(382..1416)
                     /gene="amrS"
                     /locus_tag="PLT69_05870"
     CDS             complement(382..1416)
                     /gene="amrS"
                     /locus_tag="PLT69_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOJ14001.1"
                     /translation="MQQARFYEKVRGSSVRCTLCPHRCLIADGKTGICGVRKNYGGTL
                     MSLVWARSVAANVDPIEKKPLFHFLPGSRSFSIATVGCNMRCAFCQNSDISQYAVETG
                     SVTGDVFPPEAVAASAVRAGCRSISYTYTEPTVYLEYALDTAALAREKGLYNILVTNG
                     YTSVEVVASACKHLVDAANIDLKAFTESFYKRLCSARLAPVLDAIRAYFDAGVWIEIT
                     TLVIPGENDSDAELRDIASFICSLSPDIPWHVSRYYPRHLYDKAPPTPVKTLERAREI
                     GLSQGLHFVYTGNVAGHEGEHTVCPGCRNVVIARRGFTVESMNLSGSACAACGRTIPG
                     KFTEPRVIPH"
     gene            complement(1418..3076)
                     /gene="ilvD"
                     /locus_tag="PLT69_05875"
     CDS             complement(1418..3076)
                     /gene="ilvD"
                     /locus_tag="PLT69_05875"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOJ14002.1"
                     /translation="MQSDRTKKSLEKAPHRSLLKANGLTDEEIARPFVGVVNSASEII
                     PGHIHLRSVADAVKAGVRMAGGTPMEFPVIGICDGIAMNHAGMRYSLPSREIIADSIE
                     LVAMAHAFDALVFIPNCDKVVPGMLMAALRLNVPAIVVSGGPMLAGSFRGAAVDLISV
                     FEGVGRVKAGTMTKEELAELEGCACPGAGSCAGMFTANSMNCLTEALGLGLPGNGTIP
                     AVFSERIRLAKTAGMKVMELLEKGVRPRDIATREAFANAIAVDMALGCSTNTVLHVPA
                     VAYEAGIALDLGDFDAMSRRVPHLCSLSPAGPHRLEDLYRAGGVQAVMKRLAEAKALD
                     LSVMTATGRTLRENLKSARILDDEVIRPLSKAYHKEGGIAILKGTLAPDGAVVKQSGV
                     LPDMMRRTGTARVFDSEESAVKVILAGGIKKGDVVVIRYEGPKGGPGMREMLTPTSAI
                     AGMGLDRDVALVTDGRFSGGTRGAAIGHCSPEAAEGGPIALVEDGDRIRIDIPARRLD
                     LLVDDAELARRRKAWRRPAKRLTGVLKRYARMSLSADKGARYED"
     gene            complement(3164..3364)
                     /locus_tag="PLT69_05880"
     CDS             complement(3164..3364)
                     /locus_tag="PLT69_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14003.1"
                     /translation="MDLKADIVSLAVTAAVSLVAGGAIVLMVERIEPGLVTSRGQTFL
                     FASIGVFAANLIIETIHSRMRR"
     gene            3582..4103
                     /locus_tag="PLT69_05885"
     CDS             3582..4103
                     /locus_tag="PLT69_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 family protein"
                     /protein_id="HOJ14004.1"
                     /translation="MDKTVLKRNILFGLLLVSFIIVFEIILARLHLPAWPAFLVMIQF
                     FVVHEDLAKAPEIIVGGLFGIVCAVLIDPFKAASGPILGMEAAKLSFIGAFVYAIVML
                     KDLLPHVFNSHAFLFFLVSSIAKASGDHPRPYVWMIVELVFGLVFVAGILGMNRVVES
                     VLAGADTAGRERA"
BASE COUNT          629 a         1383 c         1401 g          777 t
ORIGIN      
        1 gtgggtcttg agcatggcga tctcggtgtc ctcgtagagg ggcttggcct tgaggtcgag
       61 cctggggctg tccaggtgcg ccaccacgat cctgaggccc tcctcgaggg gcctggcccc
      121 ggccctgcac agggcgagcg ccctgccgcg gttctccatg cagacgaggt tcttgtcctt
      181 ggatgcgcgc tgcctgatgt aggatacggc ctcgcgctcg gtcttcaccg acgagaggaa
      241 cgccgtgtag tccctggcgt acgcctcgat gcggtcgttc ttgccctcga ggagctccca
      301 gcccgacacc ggtttgtacg catgcgttgt cttctttcct ggtgcggtct tgtgtgaagg
      361 catcgttgtc tcccgcgttc tctagtgggg tatcactcgg ggctcggtga acttccccgg
      421 gatggtgcgg ccgcacgcgg cacacgcgga accggagagg ttcatgctct cgacggtgaa
      481 ccccctgcgt gcgatcacca cgttcctgca gcccgggcac acggtgtgtt cgccctcgtg
      541 ccccgcgacg ttcccagtat acacgaaatg gagcccctgg ctcaatccga tttccctggc
      601 gcgctcgagg gtcttcacgg gtgtcggcgg cgccttgtcg tagaggtggc gcgggtagta
      661 cctgctcacg tgccagggga tgtccgggct cagggagcag atgaacgacg cgatgtccct
      721 cagttccgcg tccgagtcgt tctcccccgg gatcacgagg gtggtgatct ctatccagac
      781 gcctgcgtcg aagtacgccc gtatcgcgtc gagcacgggc gcgagcctcg cggaacagag
      841 cctcttgtag aacgactcgg tgaaggcctt gagatcgatg ttcgccgcgt ccacgaggtg
      901 cttgcatgcc gaggcgacga cctccaccga ggtgtagccg ttcgtgacca ggatgttgta
      961 caggcccttt tcgcgtgcga gtgcggcggt gtcgagggcg tactcgaggt acacggtggg
     1021 ctcggtgtag gtgtacgaaa tgctcctgca gcccgccctc acggccgagg ccgccaccgc
     1081 ctcgggcggg aagacgtccc ccgtcacgga gcccgtctcc accgcgtact gggatatgtc
     1141 gctgttctgg cagaaggcgc acctcatgtt gcaccccacc gtcgcgatcg agaaggacct
     1201 ggaccccggg aggaagtgga acagcggctt cttctcgatg gggtcgacgt tggccgccac
     1261 gctcctcgcc cacacgaggc tcatgagcgt gcccccgtag ttcttgcgca cgccgcagat
     1321 ccccgtcttg ccgtcggcta tcaggcacct gtggggacag agcgtgcagc ggacgctcga
     1381 tccccggacc ttttcgtaga aacgggcctg ctgcatgtca gtcttcgtag cgcgccccct
     1441 tgtccgcgga caggctcatg cgggcgtatc gtttgagcac gcccgtcagc ctcttggcgg
     1501 gcctcctcca cgccttcctc cgcctggcga gctccgcgtc gtcgacgagg aggtcgagcc
     1561 tgcgggccgg gatgtcgatc cgtatccggt caccgtcctc gacgagggcg atggggccgc
     1621 cctcggccgc ctccggcgag cagtgaccta tggccgcgcc cctggtgccg ccggagaacc
     1681 tgccgtccgt gacgagcgcc acgtcgcggt cgagccccat gccggcgatc gccgaggtgg
     1741 gcgtgagcat ctcgcgcatg ccggggccgc ccttgggccc ctcgtaccgg atcacgacca
     1801 cgtcgccctt cttgatgccg ccggcgagga tgaccttcac cgccgactcc tccgagtcga
     1861 agacccgcgc cgtacccgtg cgcctcatca tgtcggggag caccccggac tgcttcacca
     1921 cggcgccgtc aggggccagc gtccccttga ggatcgcgat gccgccctcc ttgtggtagg
     1981 ccttggacag gggccgtatg acctcgtcgt cgaggatgcg cgcggacttc aggttttccc
     2041 tgagggtcct gccggtcgcc gtcatgacgg agaggtcgag cgccttcgcc tcggccaggc
     2101 gtttcatgac cgcctgcacc ccgcccgccc ggtaaaggtc ttccagccgg tgggggcctg
     2161 cgggcgagag ggagcacagg tgcgggacac gcctgctcat cgcgtcgaag tccccgaggt
     2221 cgagggcgat gcccgcttcg taagcgaccg cggggacgtg gagcaccgtg ttcgtggagc
     2281 acccgagggc catgtccacg gcgatggcgt tggcaaaggc ctcgcgcgtc gctatgtcgc
     2341 gcggccggac gcccttttcc aggagctcca tgaccttcat gccggctgtc ttcgcgaggc
     2401 ggatgcgctc cgagaagacc gccgggatgg tgccgttgcc cggcaggccc agccccagcg
     2461 cctcggtgag gcagttcatg gagttggccg tgaacatgcc ggcgcaggaa cccgcccccg
     2521 ggcaggcgca cccctcgagc tcggcgagct cttccttggt catggtcccc gccttgaccc
     2581 tgcctacccc ctcgaacacg ctgatgaggt ccacggctgc gcccctgaaa gacccggcga
     2641 gcatgggccc gcccgagacc acgatcgcag gcacgttgag cctgagggcg gccatgagca
     2701 tgcccgggac caccttgtcg cagttgggga tgaacacgag ggcgtcgaag gcgtgggcca
     2761 tggccacgag ctcgatcgag tccgcgatga tctcgcgcga cgggagggaa tatcgcatgc
     2821 ccgcgtggtt catggcgatg ccgtcgcaga tgccgatcac ggggaactcc atgggggtcc
     2881 ccccggccat gcggaccccg gccttgaccg catccgcaac ggatctcagg tggatgtgcc
     2941 cgggaatgat ctcgctggcc gagttcacca cacccacaaa cggcctcgcg atctcctcgt
     3001 ccgtgagccc gttcgccttg agcaggctcc tgtgcggggc cttttccagg gacttcttgg
     3061 tacggtcgct ctgcatggtc catcctttct cgcgtgggat gggaacacag tatggcacct
     3121 ttggggggcg gtttcaagct cgtggaaccg ggggaggagc ctcctagcgc ctcatgcggg
     3181 agtgtatcgt ctcgatgatg aggttcgccg cgaagacccc gatggaggcg aacaggaagg
     3241 tctgccccct ggacgtgacg agacccggct cgatgcgctc gaccatgagc acgatggccc
     3301 cgcccgcgac gagagagaca gccgcagtca ccgccaggga tacgatgtcg gccttgaggt
     3361 ccatgcccgc cccccttcac agggctcgca tcgactatac cgtggatgcg ccctgcgggc
     3421 aacgccgagg cgccgcctgg gtccgaaacg agatgcagac aaccggcgcc gagttctgct
     3481 agtatccggg aaacgggcga aggggggcag aaggacagcc gcagggctgc cttgccgaag
     3541 gcctcggcct cgcccgatat cgaacggagg ggctcaagcg tatggacaag accgtcttga
     3601 agaggaacat cctgttcggg ctgctgctcg tctcgttcat catcgtgttc gagatcatcc
     3661 tcgcaaggct ccacctgccg gcctggccgg cgttcttggt catgatccag ttcttcgtcg
     3721 tccacgagga cctggcaaag gccccggaga tcatcgtggg ggggctcttc gggatcgtgt
     3781 gcgccgtgct catcgacccg ttcaaggcgg cgtcagggcc catcctgggc atggaggcgg
     3841 cgaagctgtc gttcatcggg gcgttcgtct acgccatcgt catgctgaag gacctgctgc
     3901 cccacgtgtt caacagccat gcgttcctgt tcttcctcgt ctcctccatc gccaaggcct
     3961 caggtgacca cccgaggccc tacgtgtgga tgatcgtgga gctcgtcttc gggctcgtgt
     4021 tcgtggcggg gatcctcggc atgaaccgcg tcgtggagtc cgtcctggca ggggccgaca
     4081 cagccggccg ggagcgtgcc tgagagatgc gttccccatt gtcgttcgat ctgcgtcacc
     4141 ctcttcggcg gtgcgggtgt ggcaggaagg agagggctcg aaggcgtggg
//
LOCUS       DAOSCR010000188         4173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13433_length_4173_cov_3.066780, whole genome shotgun sequence.
ACCESSION   DAOSCR010000188 DAOSCR010000000
VERSION     DAOSCR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13433_length_4173_cov_3.066780"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLT69_05890"
     CDS             complement(<1..1171)
                     /locus_tag="PLT69_05890"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOJ14005.1"
                     /translation="MESLRVLFCSSEVTPYAKTGGLADVSAALPAELRRRGVDCTVVM
                     PLYREIMNARLQLRPRFTLSVLTGAGPTEAQVYSHKEILFISHPLFSDRTGLYSYSGY
                     DYPDNLERFSLFSRACAELVRALGNIDVVHCNDWQTALVPAYLRDSLQTEVGCLFTIH
                     NLAYQGLFDAVLWPMLFLPHEYFNPECMEFYGRINVMKAGIVFSDAVNTVSPTYAREI
                     QTPAFGCGLEGLLSSVSHKLTGIVNGIDTDTWNPETDEHIAGTYSKADLRGKALCKRS
                     LMDAFSLDPTDAPVFGLIGRLVEQKGVDCVLEVIPGLLQEGARVVVLGNGNPAYEKRL
                     KELEASLKGRMGVHIGFDERLAHMIEAGSDFFLMPSRFEPCGLNQMISMRYGTIPI"
     gene            1469..2065
                     /locus_tag="PLT69_05895"
     CDS             1469..2065
                     /locus_tag="PLT69_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14006.1"
                     /translation="MTREEFERTLVDFETKIKKRLPSVIGAYLANKGNREFEASFTHL
                     VDTLDRQRKTLLRDLPKVARLDQRTRYFNAVSALDAQLRSMANKDALKEKLRLRQRRV
                     HEPVIYDLGEGPRAGLLLNAEGTGVVLETEEKIPPECEVSIELAGRKARGKAMWSIPE
                     ESGRAETGVRLASPPDEFVAEINRIISSLEGGPKGCVD"
     gene            2110..3303
                     /locus_tag="PLT69_05900"
     CDS             2110..3303
                     /locus_tag="PLT69_05900"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOJ14007.1"
                     /translation="MCTLGCKVNQYDSQKIREGLAAQGHSEARFGEAVDMVVVNTCTV
                     THRADADGRRAVRKALRTGAEVVVTGCQAVVYPEALRRAFPSARVVRPDHVPTVLGIE
                     TPRFVSGLKGHSRAFVVVQTGCDRFCTYCVVPHARGVPRSRPWQEVVGEVAHLVNLGV
                     EEVVLTGVNTGLYEGGLTRLVQKVLRYTSVKRVRLSSLEPFTVEDCLADLLADEPRVC
                     AHLHLSVQHASDRILASMGRPLSSRALRSLVELRSSSVLGLSLGADVIVGFPGETERD
                     FLETYAFFEELPFSYLHVFPFSPRPGTRASSMEARVDPGVVSMRSRALRSLSQRKRRA
                     FAASRVGSVEEVLVTRAEEGGVFGVSSTYLGVFSSTKIEPGGIVPVRITGVQGHVLTG
                     EPLVQ"
     assembly_gap    3487..3496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3497..>4173
                     /locus_tag="PLT69_05905"
     CDS             <3497..>4173
                     /locus_tag="PLT69_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ14008.1"
                     /translation="IGLRTVDAGNYVTSGDTAFLDELKSRLPSGYAELRKVQGLGPRR
                     IRRLFEEYGIQGLEDLASAIDEGRLAGSKGFTEKTLDKLRRSVAEALSYRGLFLLDEA
                     WAWMETIRGVLAGAGIEAEPTGESRRGMEVLGRVELLCAGGREEALGVASALEAFTGR
                     RPELSEGRIAVGLPGRPELVVHLCPEGSRATALFFTTGAPEHVEQLVARSAIESILVT
                     KTMVSRRG"
BASE COUNT          726 a         1254 c         1482 g          701 t
ORIGIN      
        1 cgatggggat ggtcccgtac ctcatgctga tcatctggtt gaggccgcac ggctcgaacc
       61 tggagggcat gaggaagaag tccgaccccg cttcgatcat gtgtgcgagc ctctcgtcga
      121 agccgatgtg gacccccatc cggcccttga gcgaggcctc gagctctttc aaccgtttct
      181 cgtacgcggg gttcccgttg ccgaggacca ccaccctcgc gccctcctgg aggagccccg
      241 ggatgacctc cagcacgcag tcgacgccct tctgttccac gaggcggccg atgagaccga
      301 agacgggtgc gtcggtcgga tccagggaga aggcgtccat gagggatctc ttgcagagcg
      361 cctttcccct caagtcggcc ttcgagtacg taccggctat gtgttcgtcg gtctccgggt
      421 tccaggtgtc ggtgtctatg ccgttgacga tccccgtgag cttgtgcgag accgaggaca
      481 ggagcccctc gaggccgcac ccgaacgccg gtgtctggat ctcgcgggcg tacgtggggc
      541 tgaccgtgtt gaccgcgtcc gagaacacga tccccgcctt catgacgttg atcctgccgt
      601 agaactccat gcactcgggg ttgaagtatt cgtggggcag aaagagcatg ggccacagga
      661 cggcgtcgaa gagaccttgg taggcgaggt tgtggatcgt gaagaggcat ccgacctccg
      721 tctggaggga gtctctgagg taggccggta caagggcggt ctgccagtcg ttacagtgga
      781 ccacgtcgat gttgccgagg gcccgcacga gctcggcgca tgccctcgag aacagggaga
      841 agcgctcgag gttgtcgggg tagtcgtacc ccgagtagga gtagaggccc gtccggtcag
      901 agaagagggg gtgggagatg aacaagatct ccttgtggga atacacctgt gcctccgtgg
      961 gcccggcccc ggtgagcacg gagagggtga agcgcggcct cagctggagg cgcgcgttca
     1021 tgatctccct gtagaggggc atgaccacgg tgcagtccac cccccggcgc ctgagctcgg
     1081 cgggcagggc ggcgctcacg tcggcaaggc cccctgtctt ggcgtacggc gtgacctccg
     1141 aggaacagaa cagcaccctc aatgattcca tgtgtctatc atacacgagt ggccgggccc
     1201 gagtaaaggg acacgaccgg tgagccggca gtccctccgg ccggggatgc cccggcggcg
     1261 tcttcctgcc atgtcgcctc catcgtgcac cgacgcatcg ctccgccttg acggcgggcg
     1321 gacctcttcc ccggtgggaa gggtacgggg ccggggttcc cccgctatcc ccatggaatc
     1381 gaagggcagg tgaagggagg agtgtgccgg ggggcggaag gcgaaccccc ggtttgcttt
     1441 ctgtgcacgc gtggtgtatc gttcgtgcat gaccagggaa gagttcgaac gcaccctcgt
     1501 ggatttcgag accaagatca agaagaggct cccctcggtc atcggcgcgt acctggccaa
     1561 caagggcaac agggagttcg aggcctcctt cacgcacctg gtggacacgc tcgacaggca
     1621 gcgcaagacc ctcctcaggg atctcccgaa ggtcgcgagg ctggaccaga gaaccaggta
     1681 cttcaacgcg gtgtccgccc tggacgccca gctgaggagc atggcgaaca aggacgcctt
     1741 gaaggagaag ctcaggctca ggcagaggcg tgtgcatgag ccggtgatct acgacctcgg
     1801 ggaagggccg agggccgggc tcctcctcaa cgccgagggc acgggcgtcg tgctcgagac
     1861 cgaggaaaag atccctccgg aatgcgaggt gtccatagag ctcgcaggca ggaaggccag
     1921 ggggaaggcg atgtggtcca tacccgagga gtcgggaagg gccgagacgg gggtgaggct
     1981 cgcaagcccc cccgacgagt tcgtcgccga gatcaaccgc atcatcagct ccctggaggg
     2041 gggccccaag ggctgcgtcg attgacactg gatgcagccc catagtatgc gtgccacatg
     2101 agattttcca tgtgcaccct cgggtgcaag gtcaaccagt acgactccca gaagatccgt
     2161 gaaggcctgg cggcccaagg ccacagtgag gcccgcttcg gcgaggcggt cgacatggtc
     2221 gtcgtgaaca cgtgcaccgt gacccatcgc gcggatgcgg acgggaggag ggccgtccgg
     2281 aaggccctgc gcaccggggc cgaggtggtg gtgacgggat gccaggccgt ggtgtacccg
     2341 gaagccctca ggcgggcgtt cccgtcggcc agggtcgtca ggccggacca cgtccccacg
     2401 gtcctcggga tagagacgcc gaggttcgtc tcgggactca agggccactc cagggccttc
     2461 gtcgtggtcc agaccggatg cgaccgtttc tgcacgtact gcgtggtgcc ccacgccagg
     2521 ggcgtcccgc gcagcaggcc ctggcaggag gtcgtcggag aggtggcgca cctggtgaac
     2581 ctcggggtcg aggaagtggt cttgaccggt gtcaacaccg ggctctacga gggggggctc
     2641 acccggttgg tccagaaggt cctcaggtac acaagcgtca agagggtccg cctgagttcc
     2701 ctcgagccct tcacggtcga ggactgcctc gcggatctgc tggcggacga gccgagggtg
     2761 tgcgcccacc tgcacttgag tgtccagcac gccagcgaca ggatcctcgc atccatggga
     2821 aggccgctct cgtccagggc gttgaggtcc ctcgtggagc tccgctcatc tagtgttttg
     2881 ggcctgtccc tcggcgcgga cgtgatcgtg ggtttccccg gcgagacgga gcgtgacttc
     2941 ctcgagacgt acgcgttctt cgaggagctg cccttctcgt acctccacgt gttccccttc
     3001 tcgccgaggc cggggacgag ggcgtcgtcc atggaggcgc gggtggatcc gggggtcgtg
     3061 tccatgaggt cgcgggcctt gaggtcgctc tcgcagagga aacgaagggc ttttgcagcc
     3121 tcgagggtgg gctcggtgga ggaggtcctc gtgacgaggg cggaagaagg cggggtcttc
     3181 ggcgtctcgt cgacgtacct cggggtcttc tccagcacga agatcgagcc cggcggcatc
     3241 gtgcccgtga ggataaccgg ggtccagggt catgtcctta ccggggagcc ccttgtccaa
     3301 tgaccagatg gcgggactcc tcctcgaggc cgctgaagcc ctcgagtttc tggaggagaa
     3361 ccccttcaag gcgaaggcct tcaggaaggc cgcagagtcc atcgtccggc tcgacaagga
     3421 cgtcgccgac ctcgtggctg acgggacgat ccacctcgtc aaggggatcg gaccgtccat
     3481 cgcctcnnnn nnnnnnatcg gtcttcgcac cgtggatgcg ggcaactacg tgacctcggg
     3541 cgacacggcg ttcttggacg agctcaagtc caggctcccc tccgggtacg cagaactgag
     3601 gaaggtgcag ggcctgggcc cccggcggat acggaggctg ttcgaggaat acggcatcca
     3661 ggggctcgaa gacctggcct ctgccataga cgaagggaga ctggccggat ccaaggggtt
     3721 caccgagaag accctcgaca agctgaggag gtcggtcgcc gaggcgttgt cgtaccgtgg
     3781 actcttcctc ctggacgagg cctgggcatg gatggagacg atcagggggg tcctcgccgg
     3841 ggcgggtatc gaggccgagc ccacgggaga gtccaggcgc ggcatggagg tgctggggcg
     3901 ggtggaactc ttgtgcgcgg gaggccggga agaggccctg ggtgtggcat cggcgctcga
     3961 ggccttcacg gggcggaggc cggagctctc ggaagggcgc atcgccgtgg gcctccccgg
     4021 caggccggag ctggttgtcc acctgtgtcc agagggctcg agagcgacgg ccctgttctt
     4081 cacgacagga gcgccagaac acgtggagca gctcgtggcg cgctcggcca tcgagtcgat
     4141 cctcgtcacg aagacgatgg tctcgaggcg ggg
//
LOCUS       DAOSCR010000189         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13450_length_4170_cov_3.175456, whole genome shotgun sequence.
ACCESSION   DAOSCR010000189 DAOSCR010000000
VERSION     DAOSCR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13450_length_4170_cov_3.175456"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..692)
                     /locus_tag="PLT69_05910"
     CDS             complement(243..692)
                     /locus_tag="PLT69_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14009.1"
                     /translation="MDQGESEGKGRLKAVAVIAIVTFLAGGILGYYIWGYRGHHPPDY
                     KEMLRQTISYISALEEKNRELASRVSGLENETASLKKRLGEVPAQGVQDSARTAERER
                     EYAELKAKAQERDELLKENQLLRERVRSLVEELHASGRQQGAANTSP"
     gene            complement(830..2290)
                     /locus_tag="PLT69_05915"
     CDS             complement(830..2290)
                     /locus_tag="PLT69_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOJ14010.1"
                     /translation="MDTAVDLRPKLSRVSRVHMGGPFPEPVPLVVPGLGVVRCSKILQ
                     VYERKRLTCLGLLDRHRNGGTQEDKVVVKLYFARMRAARQWRKGDRGFRVFMEGGVAA
                     PAVLFSGYLPGHGVYAAVYEYLESAENLDEVLQRTSAERDRRPMLEALIEAVALQHAS
                     GVVQKDLRASNFMVRSGNVYAIDGDLVRGFTAPLDRETSLENLAWLLANNRAFFGDHV
                     HDWYTLYCVKRGWEAEPREERRLTGEIFRVRRRNFKKTLRKVFSSRDVFVSRKERGLF
                     VLHRRDRPWLGPKEILSLIEGLPAAKGERDGSLSRYEVRSNHGGLLVFCSAGFLFSGR
                     RGFGKVGGLWRNAYMLRWLGIKAPSPLALVRSGVPMGRCVSWAVFECVEGVPLGSFLR
                     SPGVSEREMSQVLPRLARTLSTLFRFGLGLERLRSDEIMVMDDAVALSALEAVRMAVR
                     TEAAVRRLCSDFLTEWEDAPEVKSGIEASLRSEGLL"
     gene            complement(2352..3320)
                     /locus_tag="PLT69_05920"
     CDS             complement(2352..3320)
                     /locus_tag="PLT69_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ14011.1"
                     /translation="MPRIMVFGKSKRKTRKVAHIARAFRELGNETLWINAQKTRRFLG
                     PLSEPYLLMRIRRFDPDIVFIHSMDLPLTVLERISGGKAVIVMYYHDGWRLDQIEKVG
                     MWGSKVDLFLENAEGIHDRFREVGIREPVFILEGCDRHDHRVRKALLPVWKSDVAFVG
                     AAREGEARVRLVRELSRICEVKVYGKGWRKFGIKATLPEVRPRGYAKVCSGAKIVLGV
                     DAVTTIRGHWTNRLWLTLGCGGFHLTNYIPGMEEVFENRTHLVWYHSEEECVSLVQEY
                     LSKPEERKRIAMAGYRYVHEHHTFHHFAQRVLDLCGKVSKPTGERG"
     gene            complement(3699..>4170)
                     /locus_tag="PLT69_05925"
     CDS             complement(3699..>4170)
                     /locus_tag="PLT69_05925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ14012.1"
                     /translation="SVVSSLRDDVVFVLVGDGDMRAELEAEARALRLANVVFEGYRDD
                     LAPYYRVFDVFLLTSRQEGLGSSILDAMACGVPVVATRAGGIPEIVHDGETGLLVDVG
                     DAEGVARAVLRMLHDERLRRGCIDAGRRMVREGYTVEAMAKSYHDLYAELVQGA"
BASE COUNT          701 a         1516 c         1214 g          739 t
ORIGIN      
        1 ccgcctcggc ggtggggagg accgtggaac acgtgcttga cacaatcggc gcaaggctgg
       61 gggggtggtg acgcgaacgg gcctgtctcc ctcgagggac gggttccggc agcgggatcg
      121 aggacgccaa gcggtgtgga taccatcgga agccggaccc cgggcccggt tccggccacc
      181 gtcacgaccc tgtcgcagga cgcggacgag gaatgacgat cgcgcccttg cccgagggag
      241 gctcagggag aggtgtttgc ggcgccttgt tgcctgccgg acgcgtgcag ctcctccacg
      301 agggatcgga ccctctcgcg aagcagctgg ttctccttga ggagctcgtc acgctcctgg
      361 gcctttgcct tgagctcggc gtactcccgc tcccgctcgg cggtccgggc agagtcctgc
      421 acgccctggg ccgggacctc gccgagcctc ttcttcaacg aggcggtctc gttctccagg
      481 ccgctcacgc gggatgcgag ctcgcggttc ttctcctcga gcgcggagat gtaggatatg
      541 gtctgcctga gcatctcctt gtaatccggt gggtgatgcc cccggtaccc ccagatgtag
      601 tacccgagga tcccgcctgc caggaacgtg acgatcgcga tgacggcgac cgccttcagc
      661 cgccctttcc cctccgactc gccctggtcc atcgtgatct cctttcccgg ggcacaggct
      721 ccggaggcct tgcgggcggc cgggaaaggc cgcctcgaga ggcgctcctc gtccatgcgc
      781 ggggcatagc gtcatgcctg gtggggcatg gccgcgagaa gggatggtat cataaaaggc
      841 cctcgctcct caacgaggcc tcaatccccg acttgacctc aggcgcgtcc tcccactcgg
      901 tcaagaagtc ggaacagagc cttctcacag ccgcctccgt cctcacggcc atgcgcaccg
      961 cttccagcgc cgacagcgcg accgcgtcgt ccatgaccat gatctcgtcg gacctcaggc
     1021 gctcgagccc caatccgaac ctgaacaggg tcgagagggt acgggcgagc ctcgggagga
     1081 cctgcgacat ctctctctct gagacaccgg gcgaacggag gaagctgccg agaggcaccc
     1141 cctcgacgca ctcgaagacc gcccagctca cgcatctccc catgggcacg ccgcttcgta
     1201 ccagcgccag gggcgacggg gccttgatgc ccagccaccg caacatgtag gcgttcctcc
     1261 acagcccgcc cactttcccg aagcccctcc tcccggaaaa caggaaccct gccgaacaaa
     1321 acacgagcaa tccgccgtga ttcgagcgca cctcgtatcg ggacagggag ccatccctct
     1381 ctcccttggc ggcgggaagg ccctcgatga gcgagaggat ttctttcggc cccagccagg
     1441 gcctgtccct ccggtggagc acgaaaaggc ccctctcctt tcttgagacg aagacatccc
     1501 tcgaggaaaa caccttacgg agggttttct taaagttcct cctcctgacc cggaagatct
     1561 cccccgtaag gcgcctctcc tcccggggtt cagcctccca ccccctcttc acgcagtaca
     1621 gggtgtacca gtcgtggaca tggtctccga agaaggcccg gttgttggcc aaaagccacg
     1681 cgaggttttc cagggaagtc tcacggtcca atggtgctgt gaaacccctc acgaggtcgc
     1741 cgtcgatggc gtagacgttt cccgacctca ccatgaagtt ggacgccctg agatccttct
     1801 ggacgacgcc ggatgcatgc tggagggcca ccgcttcgat gagggcctcg agcatggggc
     1861 gcctgtcccg ttcggccgaa gtcctttgaa gcacctcgtc caggttctcg gccgactcca
     1921 ggtattcgta cacagcggcg tagaccccat ggccggggag gtacccggaa aagagcactg
     1981 caggtgcggc cacgccgcct tccatgaaga ccctgaaccc ccggtccccc ttcctccatt
     2041 gcctcgccgc ccgcattctc gcgaagtaga gcttgacgac gaccttgtcc tcttgcgtgc
     2101 cgccgttgcg gtgcctgtcg agaagaccga ggcacgtgag cctcttgcgc tcgtagacct
     2161 ggagtatctt cgagcacctc acgacgccga gccccgggac gaccaggggc acgggctcgg
     2221 gaaacggccc gcccatgtgc accctcgata cgcgggagag cttcggcctg aggtcaacag
     2281 cggtatccac tggcttctcc ctcgcttttg tccggcaccg tcctttcggt ctcggggtcg
     2341 agccctcgag actacccccg ctcgcctgta ggcttcgaaa ccttcccgca caggtccagc
     2401 accctctgcg caaagtggtg gaaggtgtgg tgttcgtgca cgtaccggta gcctgccatg
     2461 gcgatcctct tcctctcctc gggcttcgac aggtactcct gcacgaggct gacgcactcc
     2521 tcttcgctgt ggtaccacac gaggtgggtc ctgttctcga acacctcctc catgcccggg
     2581 atgtagttgg tgaggtggaa cccgccgcac ccgagcgtca accacagccg gttcgtccag
     2641 tgccccctga tggtcgtgac cgcatcgacg ccgaggacga tcttcgcccc cgagcacacc
     2701 ttggcatacc cccggggccg cacctccggc agggtcgcct tgatgccgaa cttcctccag
     2761 ccctttccgt agaccttcac ctcgcatatc cgcgagagct ctcttaccag tctgacccgt
     2821 gcctccccct cccgcgccgc acccacgaac gccacgtcgg acttccagac ggggagcaag
     2881 gccttcctca ccctgtgatc atgcctgtcg cagccttcga gaatgaagac gggctcgcgt
     2941 atgcccacct ctcggaacct gtcgtggata ccctcggcgt tctccaggaa gagatccacc
     3001 ttgctgcccc acatccccac cttctcgatc tggtcgagcc tccagccgtc gtgatagtac
     3061 atgacgatca ccgccttccc gccggaaatc ctctcgagaa ccgtgagcgg caggtccatg
     3121 ctgtggatga acacgatgtc cgggtcgaac ctccggatcc tcatgaggag gtacggctcg
     3181 gaaagagggc ccaagaacct cctcgtcttc tgggcattga tccacagggt ctcgttgccc
     3241 agctccctga aggccctcgc aatgtgtgca accttcctcg tcttcctctt cgacttgccg
     3301 aagaccatga tcctgggcat tcacgccctc cccgtcgtct tgaggtatcg gtcccccgga
     3361 cgccgccccc cgtctctctc cttcggcgcc caaggggcac gtgcgcgccc ccgccctttg
     3421 cggtgaggca cgacatctcc acgggccatc gccctcggat gccccggcct ctctgctcac
     3481 cgcgccttgg ggcacaaccg gaaacaggcc gcacgcggca gcgggtggga gcgtcccgac
     3541 acggtcccca ggagcagctc ggcaccaccg ggcggccacc cggtcaacaa ccggcacaac
     3601 cgtgccatgt ccggcacagg gctcatcact tccctgtccc ctcagcccat gccgaggggt
     3661 cctgccgggt gtcgtcgcgg gccgggccag gtcccacgtc acgccccctg gacgagctcc
     3721 gcgtagaggt catggtagga cttcgccatg gcctccaccg tgtacccctc gcgtaccatg
     3781 cggcgtcccg catcgatgca acctcgcctc agcctctcgt cgtggagcat cctgagcacc
     3841 gcccgtgcga cgccctcggc gtcgccgaca tccacgagca ggccagtctc gccgtcgtgg
     3901 acgatctcgg gtataccgcc cgcccgcgtc gccaccaccg gcacgccgca cgccatcgca
     3961 tccagtatcg agctgccgag cccctcctgg cgcgaggtca ggaggaagac gtcgaacacc
     4021 cggtaatagg gggcaaggtc gtccctgtac ccctcgaaca cgacgttggc aaggcgcaac
     4081 gccctggcct cggcctcgag ctccgcccgc atgtcaccgt cgcccacgag cacgaagacc
     4141 acgtcgtccc ttagggacga gaccacggat
//
LOCUS       DAOSCR010000190         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13642_length_4123_cov_3.578663, whole genome shotgun sequence.
ACCESSION   DAOSCR010000190 DAOSCR010000000
VERSION     DAOSCR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13642_length_4123_cov_3.578663"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2659)
                     /locus_tag="PLT69_05930"
     CDS             complement(<1..2659)
                     /locus_tag="PLT69_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ14013.1"
                     /translation="MKRLDLNTVDLSGTHLVEASAGTGKTYAIASLFIRLLVEAGLPV
                     ESILVITFTVPATDELKIRIRNKIREACEAFAAGDSPDAFLKGFIERSPERLQATRVL
                     EDALMRFDEASISTIHGFCRRLIADMAFETASPFRMELVPDVFEVVERAACDYYRIHV
                     SSSDLPEFVAYAMGKKVSPAWFMSLAKRPVAGRVIPGAGEPDMQTPLERYRKALDELK
                     AAWGRGRAEIEAILSDVDLIKQSSRLKAENIPGLIRVIDGFLAGGRVSMGVPECITAL
                     TYDSLSKAKKSHAVLPKHAVFDACTILEEEGKALEAAFAEKLTSLKAGFLKYLDEHLR
                     RWGEEEGRLHFDELLLRVHRALSQDTGTVLVDAVRSRYRAALIDEFQDTDPIQYEIIS
                     RCFEGLPLFFIGDPKQAVYSFRGADVFTYFKASSRVPKEKKHTLLTNYRSDPGLVEAV
                     NALFRGHDSPFLLPGISFEPAVPAQAPLRKDPGEEGEASLEIWVAREGEEYTNVSDAE
                     DAVCRAVALEIGRILSGEGPGISASNIAVLVRTNYQARKVRDVLKDARIPAVLSSDED
                     VFSTREAQETALVLDAVIEPRNEGIVRCALMTRIFGLTWRDVLALNADTASWERRLES
                     LAAYHDLWLGKGFTLMFRRLIDSEGVRERLLGMERGERALTNVLHVAELLGRAEAEGG
                     LGMRALRKWLALKMDPRAPKPEESQLRLESDDEAVKVMTVHKSKGLEFEVVFCPFAWR
                     TNRPKNDLSCWFHDENGDVVLDLGSKDWDEHRCRESQEMMAEDLRLFYVSVTRAKKRC
                     YVALVDVKNAKKGNVPEASAPHLLLGADGGGGIQEGLRGLVERAKGRIRVVPMPRGAH
                     AFPREGARGAEPVARAFTGIVDRSWRLVSF"
     gene            complement(2662..>4123)
                     /locus_tag="PLT69_05935"
     CDS             complement(2662..>4123)
                     /locus_tag="PLT69_05935"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF037683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOJ14014.1"
                     /translation="EGSGFLERGVTFCSMLPMRAVPFRVVCILGMNHDDYPRKDAASM
                     LDLMKKRRRRGDRSLRLEDLSLFLETLLSAREVLCLSYVGMGIQDNALKPPSVVVSAI
                     LDYLDKGFVSRGGGKASEAVLRRHHLQAFNPAYFTKADPIHSFSERDALAARALVSAG
                     PPGGPVVDAAAGAHVEAPATSAPGADGAEAVSCPGASPAPAIERVPLKGLIDFYSNPA
                     RCFLERTLGARPPRDDGPLEDSEPVTLEGLERYVVDERVLAGILEGEPKQRLFSRLRA
                     EGLLPHGEAGRVVFEEAASRAASFVERLSQLCKGVSRRVERVRAEIGRYVLEGDVELF
                     GHGVVASLRPAEPKTKDHLGMWVRLLAYRASGGHAEKAYHLGLDKVVSARPPADPRAA
                     LETLIEVYLEGLRRPVHLFPNSSYAYAEELVKGGDEEKAMAKARTKWAGDYEPSPDSD
                     DEAVQLLFHGTDPLDEEFRIFSARVFLPMVEAQKKG"
BASE COUNT          663 a         1427 c         1281 g          752 t
ORIGIN      
        1 tgaagctcac aaggcgccac gaccgatcga caatacccgt gaaggcacgg gcaacgggct
       61 cggcccctcg agctccttcc ctggggaagg cgtgcgcgcc cctgggcatg ggcacgacgc
      121 gtatgcggcc cttggccctc tcgacaagcc ccctcaagcc ctcctggatg cccccgcccc
      181 cgtccgcgcc caggagaagg tgaggcgcag aggcctcggg cacattgccc ttcttcgcgt
      241 tcttcacatc caccagggcg acgtagcatc tcttcttcgc cctcgtgaca ctcacgtaga
      301 agagcctcag gtcctccgcc atcatctctt gggactcgcg gcagcgatgt tcgtcccagt
      361 ccttcgagcc gaggtcgagc accacgtcgc cgttctcgtc gtggaaccag caggagaggt
      421 cgtttttcgg cctgttcgtc ctccacgcga aggggcagaa tacgacctcg aactcgagcc
      481 ccttgctctt gtgcacggtc atgactttga ccgcctcatc gtcgctctcg agcctgagct
      541 gcgactcttc tggctttggc gcccgagggt ccatcttcag cgcgagccac ttcctgaggg
      601 ccctcattcc gagcccgcct tcggcttccg ccctgccgag cagctccgcg acgtggagga
      661 cgttggtgag ggcccgctcg ccccgttcca tcccgaggag cctttcccga accccctccg
      721 agtcgatgag cctcctgaac atcagggtga accctttacc gagccagaga tcgtggtatg
      781 ccgcaaggga ctcgagccgt ctctcccagg acgccgtgtc cgcgttcagg gccaggacat
      841 ctctccacgt caggccgaag atgcgcgtca tgagggcgca gcggacgatc ccctcgttcc
      901 gaggctctat gacggcgtcc agaacgagcg ccgtctcctg cgcctccctc gtggaaaaca
      961 cgtcctcgtc gctcgagagc acggcgggga tgcgcgcatc cttgagcacg tccctgacct
     1021 tgcgtgcctg gtagttggtc ctgacgagca cggcgatgtt cgaggcgctg atccccgggc
     1081 cctccccgga gaggatcctc cctatctcga gggccacagc cctgcacacg gcatcctccg
     1141 cgtcgctcac gttcgtgtat tcctcgccct ctcgcgccac ccatatctcc agggaggcct
     1201 ccccttcctc cccggggtct ttgcgcaagg gggcctgcgc cggcactgcg ggctcgaagg
     1261 agatgcccgg gagcaaaaac gggctgtcat gcccgcggaa cagggcgttg accgcctcga
     1321 cgaggccggg atcgctcctg tagttggtga gcagggtgtg tttcttctcc tttggcaccc
     1381 gggacgaggc cttgaagtag gtgaagacgt cagcccccct gaagctgtag acggcctgct
     1441 tggggtcgcc tatgaaaaag aggggcagcc cctcgaagca cctgcttatg atctcgtact
     1501 ggatggggtc ggtgtcctgg aactcgtcta tgagagcagc cctgtaccgg gatctcaccg
     1561 catccacgag gacggtgccc gtgtcctgcg agagcgcccg gtggaccctc aagagcagct
     1621 cgtcgaagtg cagcctcccc tcctcctcac cccacctgcg gagatgttcg tcgaggtact
     1681 tcaggaaccc cgccttgagg ctcgtgagct tctcagcgaa ggccgcttcg agcgcctttc
     1741 cttcttcctc gaggatcgtg cacgcatcga acacggcgtg cttcggcagc accgcatggg
     1801 acttcttcgc cttcgagagc gagtcgtagg tgagggcggt tatgcactcg ggcacgccca
     1861 tggagacccg gccccctgcg agaaacccgt cgatcacacg gatgagcccg gggatgttct
     1921 cagccttgag cctggaactc tgtttgatga gatcgacatc ggagaggatg gcctcgatct
     1981 ccgccctgcc cctgccccac gccgccttca gctcgtccag ggccttccgg taacgctcca
     2041 ggggggtctg catatccggt tcccctgcac cggggatcac ccggccggcc acaggtctct
     2101 tggcgaggct catgaaccag gcggggctga ccttcttccc catcgcatac gcaacgaact
     2161 cgggcagatc cgacgaagag acgtggatcc tgtagtagtc gcacgcggca cgttccacca
     2221 cctcgaagac gtccgggacg agctccatcc tgaagggact ggcggtctcg aaggccatgt
     2281 cggctatgag acgcctgcag aacccgtgga tggtcgagat ggacgcctcg tcgaaccgca
     2341 tgagcgcgtc ctcgaggacc cttgttgcct gcagcctctc gggcgagcgc tcgatgaacc
     2401 ccttgaggaa cgcatccggg ctgtcgcccg cggcgaaggc ctcgcaggcc tccctgatct
     2461 tgttcctgat gcggatcttc agctcgtcgg tcgcgggcac cgtgaaggtg atcacgagga
     2521 tggactcgac gggaaggccg gcctcgacga gcagcctgat gaagagcgag gctatggcgt
     2581 aggtcttgcc cgtgcccgcg ctcgcctcga cgaggtgggt gcccgagagg tccaccgtgt
     2641 tcaggtcgag tctcttcacg cctatccctt tttctgcgct tcgaccatcg ggagaaacac
     2701 cctcgccgag aagatcctga actcctcgtc cagggggtct gtcccgtgga agaggagctg
     2761 gacggcctcg tcgtccgaat ccggactggg ttcgtagtcg cctgcccact ttgttctggc
     2821 cttcgccatg gccttctctt catcgccccc cttcacgagc tcttccgcgt atgcgtagga
     2881 gctgttcggg aagaggtgaa cgggcctcct caggccttcc aggtaaacct cgatcaacgt
     2941 ctcgagggcg gccctcgggt cggcgggcgg cctcgccgag acgaccttgt ccaggcccag
     3001 gtggtaggcc ttctcggcgt gcccgccgga cgcacggtac gccagcaacc tcacccacat
     3061 gcccaagtga tccttcgtct tgggctcggc cggtctcagg gatgccacga ccccgtgccc
     3121 gaagagctcc acatcgccct cgagcacgta gcggccgatt tcggcccgga ccctctccac
     3181 ccgtctcgag acgcccttgc agagctgcga gagccgctcg acgaacgatg cggcacgtga
     3241 tgcggcctcc tcgaacacga ccctcccggc ctctccgtgg gggaggaggc cctcggccct
     3301 caggcgcgaa aacagcctct gcttgggttc gccctcgagg atgccggcga ggacgcgctc
     3361 gtcgaccacg tacctctcga gcccctcgag ggtcacgggc tccgagtcct cgagagggcc
     3421 gtcgtccctc gggggcctcg cgccgagggt cctttccagg aaacagcggg cggggttcga
     3481 gtagaagtcg atcaggccct tgagaggaac gcgctcgatc gccggggctg gggatgcccc
     3541 cgggcaggac accgcctccg ccccgtccgc cccgggggcg ctcgtcgcgg gcgcctctac
     3601 atgagcgcca gctgccgcgt ccacgacggg cccgccgggt gggccggccg agaccaaggc
     3661 acgggcggcc agggcgtctc gctccgagaa gctgtggatc gggtcggcct tcgtgaagta
     3721 cgcggggttg aaggcctgga ggtggtgcct gcggagaacc gcctcggagg ccttcccccc
     3781 gccccgcgac acgaagccct tgtcgagata gtccagaatc gcgctcacga ccaccgatgg
     3841 cggcttcaac gcgttgtcct ggatgcccat cccgacgtag ctcaggcaga ggacctccct
     3901 ggccgagagc agggtctcca ggaagagcga caggtcttcc aagcgcaggc tcctgtcgcc
     3961 cctcctcctg cgtttcttca tgaggtcgag catggaggcc gcgtccttcc tcgggtagtc
     4021 gtcgtggttc atgccgagga tgcagacgac cctgaacggc accgctcgca tggggagcat
     4081 ggagcagaag gtcaccccgc gttcgagaaa cccgctgccc tcg
//
LOCUS       DAOSCR010000191         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13665_length_4118_cov_3.370416, whole genome shotgun sequence.
ACCESSION   DAOSCR010000191 DAOSCR010000000
VERSION     DAOSCR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13665_length_4118_cov_3.370416"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLT69_05940"
     CDS             <1..846
                     /locus_tag="PLT69_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ14015.1"
                     /translation="ACIGNKADVDESDLLEYLARDGQTTTIGMYLEGVARGRRFVRAA
                     AKACRRKPVVVLKSGRTELGAQAIASHTGSLAGADEVFDAVCRRTGLVRVHDSETFFD
                     TLAAFESLPLPRGKGLGVMSITGMGCVAATDACDEHGIALPGLRPATVRRLRQVVPDW
                     APVRNPIDIWSAVEQHGAAHTMRHIASCLLDQPDIDALLIIFVLMPESMFDIEQAFAD
                     VFARHPGKPVLVAAYGGTHKETRHVQEGFGKLGVPCYPTPERAVYALSRMLAYAEFRR
                     GKKAR"
     gene            complement(1159..2154)
                     /locus_tag="PLT69_05945"
     CDS             complement(1159..2154)
                     /locus_tag="PLT69_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14016.1"
                     /translation="MEAMTVRLIRRIRPGRGSTHGRVSVFPLFMDKARWVEHLVLKEA
                     LQDALAEVREVTEEGRVPELVVVNRASLPLLILDGEELEGARQNRVLNSSVLLPEKSA
                     TIIPVSCTEQGRWSYRTREFRDSDVMMSTRLRGVKNRSVRSSLEAGGAFSSDQGALWE
                     EIAAQARQSSVKSPTSAMKDVHEARRPELGDYVRRFPCQEGQQGVLVAIGGRVAGMDF
                     VARPASYALLHEKIVRSYVMDALLEEPGDDGVADASAAQAFIEDAAGSTARGFDSVGL
                     GRDIRFEGKRLFGSVLVHGRSFVHLAAFATDGVARRNGSGGMAGFRSRAAFRRPW"
     gene            2636..3604
                     /locus_tag="PLT69_05950"
     CDS             2636..3604
                     /locus_tag="PLT69_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HOJ14017.1"
                     /translation="MGTDRNTARKQRLVRVGKLLRAFIERDTAGTALLAKLLCVDVRT
                     VQRDLKALREAGIPIHEEKKGVYSLKKDLVRYGDLSVFDESELALIVAIKDLVSQLGA
                     PFERAAEDILGRVCEYGSCRPVYVKVESGTGLSTATMNRLVDAIQLGRRVSFHYAGAK
                     PHDVEAHPYRVAYFNGFWYLVAKDARDGKIKKYALNRVSGLKKLRSPAGRMPRDLDEA
                     LSSSVNVWFEKDRTIEVVVEVDSSWAHYFRRRSILPVQEIVEEAPDGSLVIRFLACTK
                     EEVVMCLKPWLPHLRVIRPAEIASLIAGELRSWLAWQEEAMARGMP"
     gene            complement(3673..>4118)
                     /locus_tag="PLT69_05955"
     CDS             complement(3673..>4118)
                     /locus_tag="PLT69_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOJ14018.1"
                     /translation="LVLTFRVEADTLALSGTEPELSWTEGLRLDRNTQVEGPVWRLSA
                     IRSPNGTRIVPQDPSRYGLVLSPDGTATVLADCNRGMGTYVLAGKAIVMRGLAFTRMI
                     CADDSLFDEYTLALKNASSCTRQGQRLLVFFDRDMGCLEFEPASD"
BASE COUNT          672 a         1411 c         1440 g          595 t
ORIGIN      
        1 gcgtgcatcg gcaacaaggc cgacgtggac gagagcgatc tcctcgagta cctggcccgg
       61 gacgggcaga ccaccaccat cggcatgtac ctcgagggtg tggccagggg caggcgcttc
      121 gtgagggcgg cggccaaagc ctgcaggaga aagccggtcg tggtcctcaa gtcgggccgc
      181 accgagctcg gcgcccaggc catagcctcc cacacggggt cgctcgccgg ggcggacgag
      241 gtcttcgacg cggtgtgcag gcgcacgggg ctggtgaggg tccacgactc cgagaccttc
      301 ttcgacaccc tggccgcctt cgagtcgctc ccgctcccca ggggcaaggg cctgggcgtg
      361 atgtcgatca cgggcatggg ctgcgtggct gcgacggacg cctgcgacga gcacggcatc
      421 gcgcttccgg gcttgaggcc cgccacggtc aggaggctca ggcaggtcgt ccccgactgg
      481 gcgcccgtgc gcaaccccat cgacatctgg tccgcggtgg aacagcacgg cgcggcgcat
      541 accatgaggc acatcgcgtc ctgcctcctc gaccagccgg acatcgacgc cctgctcatc
      601 atcttcgtgc tcatgccgga gagcatgttc gacatcgaac aggccttcgc ggacgtgttc
      661 gcccgccacc ccggcaagcc ggtgctcgtc gccgcctacg ggggcaccca caaggagacc
      721 cggcacgtgc aggagggctt cgggaagctt ggcgtcccct gctaccccac cccggagcgg
      781 gctgtctacg ccctgagccg catgctcgcc tacgcggaat tcaggagggg caagaaggcg
      841 cgctgaaaac agggaggcct tcccgaggcg tcgccggccg gggctgcccg ccgccacgcc
      901 gccgtccgca acggaccccg cacccataag ggaccggttc gtctccccgg cacgggctga
      961 tgacagggtg gctgcaagca cgctcacgac accgggcaag gggtgccccg cgacccaact
     1021 acccagccgc gggacgccgg ccggaagacg gccggtgcgg cgcggacagg cccgtacagg
     1081 ccggggcggg gcgcaggcag gggcccgccg agggggtcgc ggacagggcc cggccgcaca
     1141 cccgccccgc ccgggcagtc accagggtct cctgaaagcc gccctcgagc ggaagcccgc
     1201 catgccgccg gaaccgttgc gcctcgccac gccgtcggtc gcgaaggcag ccaggtgcac
     1261 gaaggacctg ccgtggacga gaaccgagcc gaacagccgc ttcccctcga acctgatgtc
     1321 gcggcccagc ccgacggagt cgaagcccct ggccgtggag cccgccgcat cctcgatgaa
     1381 cgcctgggcc gccgaggcgt cggcgacgcc gtcgtccccg ggctcctcca gcagggcgtc
     1441 catgacgtag ctcctcacga tcttctcgtg aaggagcgca taggaggcgg gcctcgccac
     1501 gaagtccatg cccgccaccc tgcccccgat cgccacgagg acaccctgct gaccctcctg
     1561 gcaggggaag cgcctcacgt agtcaccgag ctcgggccgc ctcgcctcgt gcacgtcctt
     1621 catggccgag gtcggcgact tcaccgagga ctgccgggcc tgggcggcga tctcctccca
     1681 cagggctccc tggtcggagc tgaaggcacc cccggcctcc agggaagacc tcacggagcg
     1741 gttcttcacg cccctcagcc tggtcgacat catgacgtcc gaatccctga actcccgggt
     1801 gcggtagctc cacctcccct gctcggtgca gctcaccggt atgatggtgg ccgacttctc
     1861 cggcaggagc acgctcgagt tcagcacgcg gttctgcctc gcgccctcga gctcctcgcc
     1921 gtccaggatg aggagcggca gcgacgccct gttcaccacc acgagctcgg gcacgcggcc
     1981 ttcctcggtc acctccctca cctctgcaag cgcgtcctgg agcgcctcct tgagcacgag
     2041 gtgctccacc cacctggcct tgtccatgaa gaggggaaag acgctcacgc ggccgtgggt
     2101 agaccctcgg cccggacgga tcctcctgat gagtctcact gtcatcgcct ccatacaaac
     2161 ctcccttcct cgccttgttc tcacccctgc cgctttccgg atctcccgcc tggcaggaac
     2221 ccccatcgta gaatggaacg ggggttcacg gaaggtgacc gcgacaggtg tgtgtcgcat
     2281 aaaatgcacg cgcgcatgtt tgcctcgcag gtcccgatgc gttaggatag gggccgggcc
     2341 cggccctcag gcgtggtcgc gggggttgca caggacgaaa ggccccgcct gggcgttggc
     2401 ggggatgccg tctatcggcc caccggcaag gcgcgaaagc cagggcgacg tcacggcgtg
     2461 tccacgccaa aggccggcgg gcgtatccgc ccggcgtcgc ggcgggccgc ctcgagcggg
     2521 gccgcctgtc tctgtcgtga cacgaagagg cgctcggggg cacccgcccc ggcggtggcg
     2581 gcagcgccct gcctgccggg ggcgccgggc ggacggttca gcgagggggt tcgaggtggg
     2641 gacggacagg aacaccgcga ggaaacagag gctcgtgagg gtggggaagc tgctcagggc
     2701 gttcatcgag cgcgacacgg cgggcacggc gctcttggcc aaactcctgt gcgtggacgt
     2761 gcgcacggtg cagcgcgacc tgaaggccct gagggaggcc ggcatcccca tccacgagga
     2821 aaagaagggc gtctacagcc tgaagaaaga cctcgtcagg tacggcgacc tgagcgtgtt
     2881 cgacgagagc gagctcgcgc tcatcgtggc gatcaaggac ctcgtgagcc agctcggcgc
     2941 tcccttcgag cgggccgccg aggacatcct gggcagggtc tgcgagtacg gctcgtgcag
     3001 gccggtgtac gtgaaggtgg aaagcggcac gggcctgagc acagccacca tgaacaggct
     3061 cgtcgatgcc atccagctgg gcaggagggt ctccttccac tacgcgggcg cgaagcccca
     3121 cgacgtggaa gcccacccct accgggtggc ctacttcaac ggcttctggt acctcgtggc
     3181 gaaagacgcc agggacggca agatcaagaa gtacgccctc aacagggtct ctggcctgaa
     3241 gaagctcagg tcgccggccg ggcgcatgcc cagggacctc gacgaggcgc tgtcctcgag
     3301 cgtgaacgtg tggttcgaga aggacaggac gatcgaggtg gtcgttgagg tggactcctc
     3361 ctgggcccac tacttcagga ggcgttccat cctgccggta caggagatag tggaagaggc
     3421 cccggacggg tccctggtca tacgcttcct cgcctgcacg aaggaggagg tggtgatgtg
     3481 cctgaagccc tggctgcccc acctgcgcgt gatcaggccg gccgagatcg catcgctcat
     3541 cgcgggcgag ctcaggagct ggcttgcgtg gcaggaagag gccatggcac gcggcatgcc
     3601 gtagaggggc gcgggccgtc tcgcgccggt tcccgcgggt gatgcgcggg ggggtgcgga
     3661 tgggccgggc ggctagtccg atgcgggctc gaactccagg caccccatgt ccctgtcgaa
     3721 gaagacgagc aggcgctgcc cctgcctggt gcaggagctc gcgttcttca gggccagggt
     3781 gtattcgtcg aagagcgaat cgtccgcgca gatcatgcgg gtgaacgcga ggccgcgcat
     3841 gacgatggcc tttcccgcga gcacgtacgt gcccatgccg cggttgcagt ctgcgagcac
     3901 ggtcgccgtg ccgtcggggg agagcaccag gccgtaacgc gaggggtcct gcggcacgat
     3961 gcgggtgccg ttgggcgacc tgatcgccga gagcctccac acggggcctt ccacctgcgt
     4021 gttgcggtcg agcctgagcc cctcggtcca gctgagctcg ggttcggtgc cggaaagggc
     4081 gagggtgtcg gcctcgaccc tgaaggtgag gaccaggg
//
LOCUS       DAOSCR010000192         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13745_length_4103_cov_3.468874, whole genome shotgun sequence.
ACCESSION   DAOSCR010000192 DAOSCR010000000
VERSION     DAOSCR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13745_length_4103_cov_3.468874"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1401
                     /locus_tag="PLT69_05960"
     CDS             217..1401
                     /locus_tag="PLT69_05960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ14019.1"
                     /translation="MARVILRPGREDSVLRRHPWIFSGAVQEVRGRPSPGETVEVLSS
                     SGAWLAFGAWSPHSQIRVRVWSFDRGAPVEGALFEERIQRCMLLRRDILGARETNAFR
                     LVHAESDGMPGLVVDSYAGFLVCQFSSAGVERHRDAIVDALEAAFSPRGVYERSDTDA
                     RTKEGLPKRTGLISGEEPPERVEIVEHGARFLVDIKRGHKTGFYLDQRENRLCARALS
                     SGREVLNCFSYTGAFGVAAMLGGANSVLNVDTSAASLESALDNARLNGIDLSRFGVKE
                     ADVFSCLRELRDDGRSFDLVVLDPPKFASTAGQVARAARGYKDINMLAFEVLKPGGLL
                     ITFSCSAHIGPALFQKIVADAALDAGREASIVRYLWQASDHPVGLAFPEGLYLKGLVC
                     AA"
     gene            1619..2509
                     /locus_tag="PLT69_05965"
     CDS             1619..2509
                     /locus_tag="PLT69_05965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ14020.1"
                     /translation="MVRVTNHGEDYELIDSGNGWKLERFGRYVLSRPCSQAIWPPRAQ
                     AAVWQAAHASFERRPGKGWQVRVPMEETWQVTMEGLIFRLKRTDFGHVGVFPEQRRLW
                     RWIVRRLGSLPEPWKREIQVLNLFAYSGGATLACAASGVRVCHLDASRGMVAWARENA
                     RLNGLEHAPVRWIVDDANKFLDRELRRSRRYDAIILDPPTYGHGANDEIFKIEDDLQA
                     LMRRCWSLLSERPLFVLLSSHTPGCTPKALENVMVSTMQRTSGSFESGEMLLEGGPGV
                     LAVPSGSYCRWLPGPGQGGP"
     gene            2583..3335
                     /locus_tag="PLT69_05970"
     CDS             2583..3335
                     /locus_tag="PLT69_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOJ14021.1"
                     /translation="MDGAVRTGTSSASRRGDPPDIRVLYEDNHLLALDKPAGLLTQAS
                     GLGRENLEDMARTWIRSTKGKPGNVFLHAVHRLDRLSSGIVLFARTSKALARLNGQMR
                     ERSIRKVYLAVVSGGPHEEEGTLEHTLRHSRMKAVCATPEDSGAHPCTLHFRVVARSR
                     GLALVEVEIVTGRYHQIRAQMSASGFPVLGDTRYGGRPVQGMKGIALHHARMEFSHPT
                     TALQVIITSPLPGDWPVFPGHPTTHGRRAGGD"
     gene            3422..>4103
                     /locus_tag="PLT69_05975"
     CDS             3422..>4103
                     /locus_tag="PLT69_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ14022.1"
                     /translation="MEPRENPGQGRIETRRGLHATVWVLGFVSLFMDVSSEMVHSLLP
                     LFMVGSLGVSVAVVGLIEGLAEATALITKVFSGVISDYVGKRKVLAVIGYGLGALTKP
                     LFALATGPGLIFSARMLDRVGKGIRGAPRDALVADITPPEQRGAAFGLRQALDTVGAF
                     VGPLMAVGLMLLWANDYRAVFAVAVIPAALAVLLLIFGVREPQRAPGAARVNPITRAN
                     LKRLSPACW"
BASE COUNT          659 a         1307 c         1452 g          685 t
ORIGIN      
        1 tgtcgtgcct atccggccat gccaggccac gcttgtcaag accaaaccca cctccagagc
       61 aaaggcgccg cgatattgag gcctgctcga ggcggtgttg tccacagcca gcgttcaggt
      121 gtaggatccg cggtagtaag gcatctcccc ctcgagcacg cccaaaccct ggcgatgccg
      181 tgggggatgc ccggacgacg aggggggaac atgggcatgg cccgggtcat tctgcgtcca
      241 ggaagggagg actcggtcct caggaggcac ccgtggatct tctcgggcgc cgtgcaggag
      301 gtcaggggcc ggccttctcc cggggagacc gtcgaggtcc tgtcctcctc gggggcatgg
      361 cttgcgttcg gcgcgtggtc tccccactcg cagatacggg tgagggtctg gtcgttcgac
      421 aggggagcgc ccgtcgaggg agccttgttc gaggagagga tccagaggtg catgctcctg
      481 agacgcgata tcctgggcgc ccgcgagacg aatgcgttca ggctcgttca cgcggagtcc
      541 gacggcatgc ccggcctcgt ggtggacagc tatgcgggtt tcctcgtgtg ccagttctcg
      601 agcgcgggcg tcgagcgcca ccgggatgcc atcgtggatg cgctcgaggc ggccttctct
      661 cccaggggtg tctacgagcg ttcggacacc gatgccagga cgaaagaagg gctcccgaag
      721 aggaccggcc tcatctcggg ggaagaaccc cccgagcggg tggagatcgt ggagcacggc
      781 gcgaggttcc tcgtcgacat caagaggggc cacaagacgg ggttctacct cgaccagcgc
      841 gaaaacaggc tgtgtgccag ggcgctctcc tcgggcaggg aggtcctcaa ctgcttttcg
      901 tataccggag ccttcggtgt cgcggccatg ctcggcgggg cgaacagcgt cctcaacgtc
      961 gacacctcgg cggcctcgtt ggagtccgcg ctcgacaacg cgcgcctcaa cgggatagac
     1021 ctctcgaggt tcggcgtgaa agaggcggac gtgttctcgt gcctgaggga actcagggac
     1081 gacggcaggt ctttcgacct cgtggtgctc gacccgccca agttcgcatc cacagcgggg
     1141 caggtcgcgc gtgcggcgcg cggctacaaa gacatcaaca tgctcgcctt cgaggtcctc
     1201 aagcccgggg ggctgctgat caccttctcg tgttccgcac acataggccc cgcgctcttc
     1261 cagaagatcg tggccgacgc ggcgctggac gcggggaggg aggcctcgat cgtgaggtac
     1321 ctctggcagg cttccgatca cccggtgggc ctcgcctttc ccgagggcct ctacctcaag
     1381 gggctcgttt gcgccgcatg aggcgggccc ttccccggcg ccgtcacccg cgcacggctg
     1441 cagggaccgg gcccggggtg ggggaactcg agcgcatcgc gccgcctgtg tcgtcaccgt
     1501 cgagatcgtc gcgaaggggt gggcggcccg aggtccgcag ggaggacgcc cgaccggaca
     1561 tggccgccgc tcgaggctgt acataccggc gcgggtgatg gtatgcctga gggcacgcat
     1621 ggtacgggta acgaaccacg gggaagacta cgagctcatt gacagcggga acggctggaa
     1681 gctcgagcgg ttcggcaggt acgtgctgtc gaggccgtgc agccaggcga tctggcctcc
     1741 gagggcccag gcggccgtgt ggcaggcggc ccacgcctcc ttcgagcgca ggccgggcaa
     1801 gggatggcag gtgcgcgtcc ccatggagga gacctggcag gtcaccatgg aagggctcat
     1861 cttccggctc aagaggacgg acttcggcca cgtgggcgtc ttcccggaac agcgcaggct
     1921 gtggagatgg atcgtcaggc ggttggggtc cctccctgag ccgtggaaga gggagatcca
     1981 ggtgctcaac ctgttcgcgt actcaggcgg tgcgacgctc gcctgcgcag cctctggggt
     2041 gcgtgtctgc cacctcgacg cctcccgcgg catggtcgcc tgggcgaggg agaacgcgag
     2101 gctgaacggt ctcgagcatg cgccggtgag atggatcgtg gatgacgcga acaagtttct
     2161 cgacagggaa ctcaggcgct cgaggcgcta cgacgccatc atcctcgacc cgccgaccta
     2221 cggtcacggc gcgaacgacg agatcttcaa gatcgaggac gacctgcagg ccctcatgcg
     2281 caggtgctgg tcgctgctct ccgagagacc cctgttcgtc ctcctctcct cccacacccc
     2341 tggatgcacg cccaaggccc tcgagaacgt gatggtctcc accatgcaga ggacgagcgg
     2401 atcgttcgag tcgggcgaga tgctcctgga aggcggcccc ggggtgctgg ccgtgccgag
     2461 cggctcatac tgcaggtggc tccccggccc cggtcaagga ggtccctgat gggcaagacg
     2521 gtgcgctcct cttccgcccg tctgtgtccc ccttgggccg tcgcggggaa agagggcccc
     2581 tgatggacgg cgcggtccgc acgggaactt cttctgcgtc tcggcgggga gaccctcccg
     2641 atatcagggt gctctacgag gacaaccacc tccttgcgct cgacaagccg gcaggcctcc
     2701 tcacccaggc gtccggcctg gggcgcgaga acctggagga catggccagg acgtggatca
     2761 ggtctaccaa gggcaagccc ggcaacgtct tcctccacgc cgtgcacagg ctcgaccgcc
     2821 tctcgagcgg gatcgtcctc ttcgcgagga cgagcaaggc gcttgcgcgg ctgaacggcc
     2881 agatgaggga gcgttccatc cgcaaggtct acctcgccgt ggtctcgggt ggcccgcacg
     2941 aggaggaggg cacgctcgag catacgctca ggcactcgcg catgaaggcc gtctgcgcga
     3001 cgccggaaga ctcgggcgcc cacccctgca ccctgcactt cagggtggtc gcgagatcca
     3061 ggggcctggc cctcgtggag gtcgagatcg tgacgggacg ctaccaccag atcagggccc
     3121 agatgagcgc gtctgggttc ccggtgctcg gcgacacacg ctacggggga aggcctgtgc
     3181 agggcatgaa ggggatcgcg ctgcaccacg cgcgcatgga gttctcacac ccgactaccg
     3241 ccctccaggt gatcatcacc tctcccttgc ccggagactg gcctgtcttt cccggtcacc
     3301 ccaccaccca cggccggcgc gcggggggcg actagcccgc gggtgagtcc ctcactgcgg
     3361 gaatgcaacg cacaggggca agggctcgtc acgggatcga catacgaagg agtagacggg
     3421 tatggaaccg agggaaaacc cgggacaagg gaggatcgag acccgcaggg ggcttcatgc
     3481 cacggtatgg gtcctggggt tcgtgagcct cttcatggac gtgtcctccg agatggtgca
     3541 cagcctgttg ccgctgttca tggtgggctc gctcggcgtg agcgtggccg tggtcgggct
     3601 gatcgagggt ctggccgagg ccacggcctt gatcaccaag gtgttctccg gcgtcatcag
     3661 cgactatgtc ggcaaacgca aggtgctcgc ggtgatcggc tacgggcttg gcgcgctgac
     3721 caagccgctg tttgcgttgg ccactggccc ggggctgatc ttttcggcgc gcatgctgga
     3781 cagggtcggc aaggggattc gcggtgcgcc cagggatgct ctggtggccg atatcacgcc
     3841 gcccgagcag cggggcgccg cattcggcct gcgccaggcg ctcgacacgg tcggggcctt
     3901 cgtcggccct ttgatggcgg tgggcctgat gttgttgtgg gccaatgact accgggcggt
     3961 gttcgccgtc gcggtgatcc cggcggcgct ggccgtgctg ctgctgatct tcggtgtgcg
     4021 cgagccgcag cgcgcgccgg gggcggcgcg ggtcaacccg attacacgcg ccaacctcaa
     4081 acgcctgagt cctgcctgtt ggt
//
LOCUS       DAOSCR010000193         4095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13788_length_4095_cov_3.401980, whole genome shotgun sequence.
ACCESSION   DAOSCR010000193 DAOSCR010000000
VERSION     DAOSCR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13788_length_4095_cov_3.401980"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..168
                     /locus_tag="PLT69_05980"
                     /pseudo
     CDS             1..168
                     /locus_tag="PLT69_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
     gene            208..1092
                     /locus_tag="PLT69_05985"
     CDS             208..1092
                     /locus_tag="PLT69_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOJ14023.1"
                     /translation="MHKAGQSCETCPDETCSAKKRKSDESDADFADRRKLEQRLCRIK
                     HKILVLSGKGGVGKSTVAVNLAVALMMDGWSVGLLDADIHGPSIPTMLGLEGERVEGE
                     PGELLPVDLGGLKVMSLGFFLRHRDDAVIWRGPMKMGALKQFLKDVAWGDLDYLIVDS
                     PPGTGDEPLSVCQLLGSVDGAVVVTTPQKVAAVDVRKSVSFCRQLKVPVLGVVENMSG
                     FVCPHCGKHTPVLKSGGGRGIAEDMGVPFLGAIPMDPQVAEAGDDGYAFVHRYAKSPT
                     AGAMRDIVEQLFLELQEE"
     gene            1144..1482
                     /locus_tag="PLT69_05990"
     CDS             1144..1482
                     /locus_tag="PLT69_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ14024.1"
                     /translation="MKIAIPIANGTLCMHFGHCERFALIDVNPETRTIMGRQDIAAPP
                     HQPGLLPPWLAERGVTFVIAGGMGQRAIALFQQQGIDVLVGAPAMAPEALVEEYLAGR
                     LQGGRNVCDH"
     gene            1541..3241
                     /locus_tag="PLT69_05995"
     CDS             1541..3241
                     /locus_tag="PLT69_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ14025.1"
                     /translation="MVDRKRIVVIGGSAAGPKAAAKARRMDEHAEVVIFQKDADLSMA
                     SCGYPYYVGGYFDDRNMLLSTPAGIVRDPKFYLNAKAIDARTLTEVTSIDRKGHTVSY
                     RHLATGETGSMGYDKLIIATGATPKMPPIPGVNLKGITTLQSMKDADFLRKVRDEGLI
                     KKAVIVGGGLIGIETCEALQLAGIDITVIELLPQLLTFLDWDLAKLVENHVRSKNANV
                     ITGNGIAEFLGENGTLTGVKLSNGTELPCELAVVAIGVSPNVKLAKEAGLAIGVTGGI
                     EVNEYMQTSDPDIYACGDCVETVHRITGKKALAPYGDLANLQGRVAGENAVLGNTVTF
                     PGTIQTGICKVFDYSAGSTGLSETAARKNGYEDIVTVMNASTDKPNYMGGELLITKLV
                     CERSTGKILGAQCIGPGNVAKQIAQWAMAIQAGMHVEDIVNADLPYAPPFSLAIDHCI
                     ATAHLMQNKLKGRLKGISPTEVRKKLDGKDRPFFLDARGPDEYEQTRLGIGETLIPLG
                     ALRKRLNELPQDKNKEIICYCKISLRGYEAALVLEANGWTNVKVMEGGIVAWPYAKEK
                     "
     gene            3328..>4095
                     /locus_tag="PLT69_06000"
     CDS             3328..>4095
                     /locus_tag="PLT69_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14026.1"
                     /translation="MKIHEVDKVEILTLQDNYIDVTALDNTAVVQRAVPLKDMQIKNS
                     ILAEHGFSALVTTHVAGKASSLIFDFGFSEDGAARNAVKLGVDMGSVKTLVLSHGHSD
                     HTGGFHEVGALITGNKSAVEFVCHPAVFKKSRYLKFGEEIKVFFPGFTKEDVLKEGFK
                     LVETAEPYPLLDGSALFLGEVQRLTDFEKGFPIAHRMEDGREQWDPIEDDTGIAVHVR
                     GKGLVVVTGCAHSGVVNTVRHAMKTTGVDRVLAVIGGF"
BASE COUNT          815 a         1283 c         1384 g          613 t
ORIGIN      
        1 ccttcacggg cctgcgggag catgggcacc tccctggcct cgggcaagga cgtgcgcgag
       61 gcagcgcaga tcgggcagca ggagatcctg aaggccctgg gaggcctgcc cgccgaggtg
      121 gagcactgcg ccctgctggc ggcgaatacc ctcaaggcgg ccctggatgc gtacggcaag
      181 ggccgcactg accgtgcagc gcacaggatg cacaaggcag gccaaagctg tgagacctgc
      241 ccggacgaga cctgctcggc gaagaagcgc aagagcgacg agagcgatgc cgacttcgcg
      301 gatcgccgga agctcgagca gaggctctgc cgcatcaaac acaagatcct ggtgctctcg
      361 ggcaagggcg gggtcggcaa gagcaccgtg gccgtgaacc tggccgtggc cctcatgatg
      421 gacggctgga gcgtggggct gctggatgcc gacatccacg gcccgagcat ccccaccatg
      481 ctcggcctgg aaggggaacg ggtggaaggc gagcccgggg agctccttcc cgtggacctc
      541 ggcgggctca aggtcatgtc gctgggcttc ttcctccgcc accgcgacga tgccgtgatc
      601 tggcgcggac ccatgaagat gggggccctc aagcagttcc tcaaggacgt ggcctggggc
      661 gacctggact acctcatcgt ggattcgccg ccgggcaccg gcgacgaacc cctgtcggtc
      721 tgccagctcc tgggctccgt ggacggcgcg gtggtggtga ccacgcccca gaaggtggcg
      781 gccgtggacg tacgcaagtc cgtctccttc tgccggcagc tcaaggtgcc cgtgctcggg
      841 gtggtggaga acatgagcgg gttcgtctgt ccgcactgcg ggaagcacac gccggtgctc
      901 aaaagtgggg gcggcagggg catcgccgag gacatgggcg tccctttcct cggcgccatt
      961 cccatggacc cccaggtggc cgaggccgga gacgacgggt acgccttcgt ccaccgctat
     1021 gccaagagcc ccacggcggg cgccatgcgg gacatcgtgg aacagctctt cctggaactg
     1081 caggaggaat aggcctgttc ccatgaaagg aaagaacgca tcatccaagc aaggaggata
     1141 gtcatgaaaa tcgccatacc cattgcgaac ggaaccctgt gcatgcattt cggacactgc
     1201 gagcgtttcg ctctcatcga cgtgaacccc gagacgagga cgatcatggg caggcaagac
     1261 atcgccgccc cgccgcacca gcccggtctg cttccgccct ggctcgccga gcgcggcgtc
     1321 accttcgtca tcgcgggcgg catgggccag cgggccatcg ccctgttcca gcagcagggc
     1381 atagacgtcc tcgtgggggc cccggccatg gcgccagagg ccctggtgga ggagtacctc
     1441 gcaggaaggc tccagggcgg ccggaacgtg tgcgaccact gaggggtcgg aaccgggggc
     1501 agggcatgaa ccaatctttg caccatcaaa ggaggatcgc ttggtagaca ggaagaggat
     1561 cgtggtcata ggcgggtccg cggcaggacc caaggcggcg gcaaaggcga gacgcatgga
     1621 cgagcatgcg gaggtggtga tcttccagaa ggacgcggac ctctccatgg cgtcctgcgg
     1681 gtacccctac tacgtgggcg gctacttcga cgaccggaac atgctcctga gcacccccgc
     1741 gggcatcgtg cgggacccca agttctacct gaacgccaag gctatcgacg cgcggacgct
     1801 caccgaggtg acctccatcg acaggaaagg ccatacggtc agctaccgac atctcgccac
     1861 cggcgagacc gggtccatgg gctatgacaa gctcatcatc gccaccgggg caacgcccaa
     1921 gatgcccccc atcccaggcg tcaacctcaa gggcattacc acgctccagt ccatgaagga
     1981 cgccgatttc ctgcgcaagg tgcgagacga gggcctcatc aagaaggcgg tcatcgtcgg
     2041 cggcgggctc atcggcatcg agacctgcga ggcgttgcag ctcgcgggca tcgacataac
     2101 ggtcatcgaa ctgttgccgc agctgctcac cttcctggac tgggacctgg cgaagctcgt
     2161 ggagaatcac gtgaggtcca agaatgccaa tgtcataacg ggcaacggca tcgccgagtt
     2221 cctgggtgaa aacggcacgc tcaccggggt gaagctgtcc aatggcacgg agctgccctg
     2281 cgagctggcc gtggtggcca tcggcgtgag cccgaacgtg aagctcgcga aagaggccgg
     2341 cctcgccatc ggggtcacgg gaggcatcga ggtgaacgag tacatgcaga catccgaccc
     2401 ggacatctat gcctgcggtg actgcgtcga gaccgtgcac cgtatcacgg gcaagaaggc
     2461 gctggcgccc tacggcgacc tggcgaacct ccagggccgc gtggcgggcg agaacgcggt
     2521 tctcggcaac acggtcacct tcccgggcac catccagacc ggcatctgca aggtcttcga
     2581 ctattcggcc gggtccacgg gcctgtccga gacagcggca aggaagaacg gctacgaaga
     2641 catcgtcacc gtgatgaacg cgagcacgga caagccgaac tacatgggcg gcgagctcct
     2701 catcaccaag ctcgtgtgtg agcgttccac cggcaagatc ctcggcgccc agtgcatcgg
     2761 tcccggcaac gtcgccaagc agatcgccca gtgggccatg gccatccagg cgggaatgca
     2821 cgtggaggac atcgtgaacg ccgacctccc gtacgcgccc cccttctcgc tggccatcga
     2881 ccactgcatc gccaccgccc acctcatgca gaacaagctc aagggcaggc tcaagggcat
     2941 ctcgcccacg gaggtgagga agaagctgga cgggaaggac aggccgttct tcctcgatgc
     3001 ccggggcccg gacgagtacg agcagacgcg ccttggcatc ggcgagacgc tcatccccct
     3061 gggcgcactg agaaaacgcc tgaacgagct gccccaggac aagaacaagg agatcatctg
     3121 ctactgcaag atctcgctca ggggctacga ggcggccctg gtgctcgagg ccaacgggtg
     3181 gacgaacgtc aaggtcatgg agggcggcat cgtggcgtgg ccgtatgcga aagagaagtg
     3241 atggcgaggc gggctgcttc cactgactgg tgagaaacgg gtgcggcagg ctgcggccgc
     3301 acccgaatga acgtgaagga gggatctatg aagattcacg aggtggacaa ggtggaaatc
     3361 ctgaccctgc aggacaacta catcgacgtc acggccctgg acaacacggc ggtggtgcag
     3421 cgggccgtgc cgctcaagga catgcagatc aagaactcca tcctggccga gcacgggttc
     3481 tcggccctgg tcacgaccca tgtggcggga aaggcgtcgt ccctgatctt cgacttcggg
     3541 ttctccgagg acggcgcagc ccgcaacgcc gtcaagctcg gtgtggacat gggctcggtc
     3601 aagacgctgg tgctgtccca cgggcattcg gaccacaccg ggggcttcca tgaggtcggc
     3661 gctctcatca ccgggaacaa gagcgccgtc gagttcgtct gccaccccgc ggtcttcaag
     3721 aagtcgcgct atctgaagtt cggcgaggag atcaaggtct tcttccccgg gttcacgaag
     3781 gaagacgtgc tcaaggaggg gttcaagctg gtggagaccg ccgagcccta cccgctcctg
     3841 gacgggagcg ccctgttcct cggcgaggtg caaaggctca ccgacttcga gaagggtttc
     3901 cccatcgccc accggatgga ggacggccgc gagcagtggg accccatcga ggacgacacg
     3961 ggcattgcgg tgcacgtgag gggcaagggc ctcgtggtcg tcaccgggtg cgcccactcg
     4021 ggcgtggtga acacggtgcg ccacgccatg aaaacaaccg gcgtggacag ggtgttggcg
     4081 gtgatcggcg ggttc
//
LOCUS       DAOSCR010000194         4086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13830_length_4086_cov_3.220293, whole genome shotgun sequence.
ACCESSION   DAOSCR010000194 DAOSCR010000000
VERSION     DAOSCR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13830_length_4086_cov_3.220293"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLT69_06005"
     CDS             <1..521
                     /locus_tag="PLT69_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HOJ14027.1"
                     /translation="EDYDAACFAVCAWIDETIMNSSWEHRQLWMKEPLQLRFYQTTNA
                     GELFFERLGGLGVHQREAREVYYLCLAMGFMGRFVREEDRFLLDQLKTSNLKVLTGSS
                     LGTPQLSSQALFPEAYPTGEAPSRKGAGRGLPLILTAAVAAVPVVLFVVLAVVYSFVL
                     GNVADALFTPRP"
     gene            518..3961
                     /locus_tag="PLT69_06010"
     CDS             518..3961
                     /locus_tag="PLT69_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HOJ14028.1"
                     /translation="MMKKIISAVIAVFAVLAAVTLAFGVVLVLAWPLWVGFFILLVMA
                     GIATAVVFARQIVLRRREERFVSSIVAQDEEAVRGLEDEERRRRTELQEKFRQAVGAL
                     RASHLRKLGNPLYVLPWYMVIGESGSGKTTAIKSARLSSPFAEAHRVQGVSGTRNCDW
                     WFFEQAVILDTAGRYAIPVDEGRDLEEWQRFLGLLARYRRREPLSGLVVTIAADRILG
                     ASPEELEEEARQIRRRMDELMRSLGSVFPVYVLVSKCDLVQGMTRFCDRLDEEALKQA
                     FGMVKKDPGEDCASFVDRALEALVERLRYLRLIALHDQGISGADPALLLFPEEFSRMK
                     TGLAAYVRGAFQENPYQETPLLRGVYFSSGRQEGAPYSHFLKALGLVSQAEVLPGTNR
                     GLFLHDFFAKVLPADRGLAAPTRRALAWQRTTLNLALMAWLAFGIGLSGLLSFSFVKN
                     LSAIRSVPASSTRAVEFSGEPSADVLTLSRYRDAVSAIHERNSGWWIPRFGLDESIEV
                     EQRLKARFCSLFDERFLRNHDARMAASIESLDATDETLGDYIISILGRIKLIRERTQG
                     GDVHKNAGSFLMMAGDTGLSRDQAATLSELYLQRVQWEDEEKLAREARVLESMLVKAV
                     KRSSSLDWLVKWVNAGNADKKVTFEDFWGEQKPDAFQASVDPAYTKEGRALVRELLAD
                     LDTVLGADAPFASRKEAFLAAYRKDCMEAWHTFAVHFPKGASGLKDAARWRESASKMA
                     TRSGPYQALLARMVEEARSIGDGDRPAWIQGLFRYQEDRELSSRLTGAGSVSKAAEAA
                     ARLKEKLQASMGGEGGTDAAGPHVESAKALREYEAALAKISASVSVSRAKAYQSASVM
                     FGEDNTASPFYAAKAALDRYAQASGIDRETLALIGGPLDFLWQYTCQEAACYLQEVWE
                     KDVLADAQGIADSMQLNQVLFAPDGLATKFIKGPAAPFVGRDVRRGFYPREVLGRTLP
                     FEASFLDFFSRARVGLSVASLAGAASDVPVSVTGYPTEANDSARVQPHATRLAVQCMD
                     SQQVFVNMNYPATKTLMWSPQRCAGVTFSIEVGNLVLTKTYTGQDAFAKFLLEFKGGA
                     RTFTPQDFPREENALKSMGIRYIKVQYRFSGHQAVISQAKPVMGQPSTLPVTIARCPW
                     R"
BASE COUNT          699 a         1287 c         1428 g          672 t
ORIGIN      
        1 aggaggatta cgacgccgcc tgcttcgcgg tgtgcgcctg gatcgacgag acgatcatga
       61 actcctcgtg ggaacaccgg cagctgtgga tgaaggaacc gctccagctc aggttctacc
      121 agacgacgaa cgcgggggaa ctcttcttcg agaggctggg cggcctcggc gtgcaccaga
      181 gagaggcccg ggaggtgtac tacctgtgcc tcgccatggg cttcatgggc cgtttcgtca
      241 gggaggagga ccgtttcctg ctcgaccagc tcaagacctc gaacctcaag gtgctcaccg
      301 gcagctcact cggaacgccg cagctttcgt cccaggcctt gttccccgag gcctacccga
      361 ccggcgaggc cccgtcccgg aagggggcgg gacgggggtt gcccttgatt ctgaccgccg
      421 cggtcgcggc ggtccccgtg gtgctgttcg tcgtgctcgc cgtcgtctac tccttcgtgc
      481 tcgggaacgt cgccgacgcg ctcttcaccc ccaggccatg atgaagaaga tcatctcggc
      541 ggttatagcg gtctttgcgg tcctcgcggc ggtcacgctt gccttcggcg tcgtgctcgt
      601 ccttgcctgg ccgctctggg tggggttctt catcctcctc gtcatggcgg gcatcgccac
      661 ggcggtcgtc ttcgcgagac agatcgtcct gagaaggcgc gaggaacgct tcgtgagcag
      721 catcgtcgcc caggacgagg aggccgtcag gggtctcgag gacgaggagc gaaggaggag
      781 gaccgagctc caggagaagt tcaggcaggc tgtcggcgcc ctcagggcgt cgcacttgag
      841 gaagctcgga aacccgctct atgtgctgcc ctggtacatg gtgataggcg agagcggctc
      901 gggcaagacc acggccatca agagcgcacg cctgtcttcg ccgttcgccg aggcccaccg
      961 ggtccagggt gtctcgggta ccaggaactg cgactggtgg ttcttcgagc aggccgtcat
     1021 cctcgacacg gccggcaggt acgccatccc cgtggacgag ggcagggacc tcgaggaatg
     1081 gcagcgcttt ctggggctcc tcgcgaggta ccggaggagg gagccgctca gcggcctcgt
     1141 ggtgaccatc gcggcggaca ggatcctcgg cgcgtccccc gaggaactcg aggaggaggc
     1201 gcggcagatc aggagacgca tggacgagct catgcggtcg ctcgggagcg tctttcccgt
     1261 ctacgtgctc gtgagcaagt gcgacctcgt tcagggcatg acgaggttct gcgaccggct
     1321 cgacgaggag gcgctcaagc aggccttcgg catggtcaag aaggaccccg gggaggactg
     1381 cgcgtcgttc gtggaccgcg ccctcgaggc gctcgtggag aggctcaggt acctgaggct
     1441 gatcgcgctc cacgaccagg ggatctcggg ggccgatccc gcgctcctgt tgttccccga
     1501 ggagttctcg aggatgaaga ccggccttgc ggcctacgtg aggggcgcat tccaggagaa
     1561 cccgtaccag gaaacccccc tcctcagggg ggtctatttc agcagcgggc gacaggaggg
     1621 ggcgccctac tcgcacttcc tcaaggccct ggggctcgtc tcgcaggcag aggtgctgcc
     1681 ggggacgaac agggggctgt tcttgcacga cttcttcgcc aaagtcctgc cggcggacag
     1741 gggactcgcg gcgccgacca ggagggcgct cgcctggcag aggacgaccc tcaacctcgc
     1801 cctgatggca tggctcgcct tcgggatcgg tctctcgggc cttttgagct tttcgttcgt
     1861 gaagaatctc tcggcgatac ggtcggtgcc cgcatcctcc acgagggccg tcgagttttc
     1921 cggtgagccg tcggcggatg tgctcacgct ctccagatac agagacgccg tgtccgcgat
     1981 ccacgagagg aacagcggct ggtggatacc gaggttcggg ctcgacgaga gcatcgaggt
     2041 ggaacagcgg ctcaaggcgc gcttctgttc cctgttcgac gagcggttcc tcaggaacca
     2101 cgacgccagg atggcggcct ccatagagtc gctggacgcc accgacgaga ccctgggaga
     2161 ctacatcatc tccatcctgg ggcgcatcaa gctcatccgg gagaggaccc agggcggaga
     2221 cgtgcacaag aacgccgggt cctttctcat gatggccggt gatacagggc tctcccggga
     2281 tcaggcggcg accctgtctg agctctacct ccagcgcgtg cagtgggagg acgaagagaa
     2341 gctcgcgagg gaggcccggg tcctggagtc gatgctcgtg aaggccgtga agcggtcgtc
     2401 cagcctggac tggctcgtga agtgggtgaa tgccggcaat gccgacaaga aggtcacgtt
     2461 cgaggacttc tggggagagc agaaacccga tgcgttccag gcgtccgtcg accccgccta
     2521 tacgaaggag gggagggccc tcgtgcgaga gctcctcgcg gacctcgaca ccgtgctcgg
     2581 cgccgacgcc cccttcgcgt cgcgcaagga ggccttcctg gccgcctacc ggaaggactg
     2641 catggaggcc tggcacacct tcgccgtgca cttcccgaag ggtgcatcgg gcctgaagga
     2701 cgccgcccgg tggagggaga gcgcctcgaa gatggcgacc cggagcggcc cctaccaggc
     2761 cctgctggca cgcatggtcg aggaggcgag gtcgatcggc gacggggaca ggccggcgtg
     2821 gatccagggc ctgttccgct accaggagga cagggagctc tcctcgaggc tcacgggcgc
     2881 aggctcggtg tcgaaggccg ccgaggcggc cgccaggctc aaggaaaagc tccaggcgag
     2941 catgggcggg gaaggcggga ccgacgcggc gggcccccac gtcgagtcgg ccaaggcgct
     3001 cagggagtac gaggccgccc tggcgaagat ctcggcttcc gtctccgtct cgagggccaa
     3061 ggcctaccag tcggcgtcgg tgatgttcgg cgaggacaac acggcttccc ccttctatgc
     3121 ggccaaggcg gccctcgaca ggtacgcgca ggcgagcggc atcgacaggg agaccctggc
     3181 gctcatcggc ggcccgctcg atttcctctg gcagtacacg tgccaggagg cggcctgcta
     3241 tctccaggag gtgtgggaga aagacgtgct cgcagacgcc cagggcatcg ccgacagcat
     3301 gcagctcaac caggtcctgt tcgctcccga cggcctggcg accaagttca tcaaggggcc
     3361 cgccgcaccc ttcgtgggca gggacgtaag gcgcggtttc tacccgaggg aggtgctcgg
     3421 caggacgctg cccttcgagg cctccttcct cgacttcttc tcccgcgcca gggtcggcct
     3481 gagcgtcgcc tcgctcgcgg gcgccgcatc cgacgtgccc gtgagcgtga cggggtatcc
     3541 caccgaggcc aacgacagtg caagggtcca gccccacgcg acgaggcttg ccgtccagtg
     3601 catggactcc cagcaggtct tcgtgaacat gaactaccct gcgaccaaga ccctcatgtg
     3661 gtcacctcag cggtgcgccg gcgtgacctt ctcgatcgag gtgggcaacc tcgtcctcac
     3721 gaagacctac acggggcagg acgcgttcgc caagttcctc ctcgagttca aaggcggcgc
     3781 gaggaccttc accccgcagg atttcccgag ggaggaaaac gccctcaagt ccatgggcat
     3841 acggtacata aaggtccagt accgtttcag cggtcaccag gcggtgatca gccaggcgaa
     3901 gccggtgatg gggcagccga gtaccctgcc ggtgaccatc gcgaggtgcc cctggaggtg
     3961 aggggagggg cccgcatggg ctgggcggcc ttcgggaagc accctgtgtc gaaggatttc
     4021 gtggccctgg gcaaggcggg gggagtggcc gaaaggatcc tcgcatgggt cgagggtggg
     4081 tacgag
//
LOCUS       DAOSCR010000195         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13924_length_4066_cov_3.537522, whole genome shotgun sequence.
ACCESSION   DAOSCR010000195 DAOSCR010000000
VERSION     DAOSCR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13924_length_4066_cov_3.537522"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLT69_06015"
     CDS             complement(<1..216)
                     /locus_tag="PLT69_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14029.1"
                     /translation="MDKIIDTLLRQKPLVKVLVLAVVLGLVVGVYWQFFFRPIQAEIA
                     AVEPELNQLKAELAAKKEIVKEKARYLA"
     gene            complement(228..761)
                     /locus_tag="PLT69_06020"
     CDS             complement(228..761)
                     /locus_tag="PLT69_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOJ14030.1"
                     /translation="MVKINLLPIRVELKKRALIEHVVLLLLCVTLVLISEYFVQHSMV
                     SRLDSLKKEIADTKVEIKRLTAEAGEIEKFKKQKQELEKKLDVIRDLNAKRTGPVEVL
                     DELSMIIPEKAWLTSFTNKGDTVVLEGAAVDNTTIAAFLKQLQASKHFDNVNLVLSRQ
                     EGGQQKFSITCKIKLSS"
     gene            complement(755..1810)
                     /gene="pilM"
                     /locus_tag="PLT69_06025"
     CDS             complement(755..1810)
                     /gene="pilM"
                     /locus_tag="PLT69_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOJ14031.1"
                     /translation="MFLEKNLLGLDIGSQSIKMVDIAKTRSGYQLKSMGIGLIPAECI
                     VDKDIIDSETVVDTIKNLRENLKVRTRGSATAISGHSVIVKKAELALMSEAELRQNLP
                     IEAEQYIPFDINDVYLDTFILGESIERSDMMDVLFVAAKKELVDEYASAVRNAGLKPM
                     LIDIDVFSLETMYEVNYPDAPESIVALVNIGASMINVNVLKDNMSIFARDISMGGRQL
                     TERIQREFNVSFERAENIKTGADIEGIDLEKINYIFKMAAETYVQEIRRTLDFFLSTM
                     VNENIEKIYISGGSSRIPGITRLLEKQMEIPVEIVNPFNNISWDERVFDPEYMAYIAP
                     QMAVAVGLALRRADYKW"
     gene            complement(1826..2113)
                     /locus_tag="PLT69_06030"
     CDS             complement(1826..2113)
                     /locus_tag="PLT69_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOJ14032.1"
                     /translation="MRTTHALILGAILVFALLLVFTLPGERGVIRGYQLHKDLTTLKS
                     TNEHLERENAALAKEATMLRDNMAYIEHVIVRELNMVRPGDTIVVFKRKKN"
     gene            complement(2281..2946)
                     /locus_tag="PLT69_06035"
     CDS             complement(2281..2946)
                     /locus_tag="PLT69_06035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ14033.1"
                     /translation="MFPVKDTTLERAFAPVTTLLILATIALYIADLRLSGGLQAISRV
                     SPLDARNSLVLGSPGLVRLHASIILSGFMHTGVLHLALNLVFLSAFAPAVERTFGWVS
                     FSVFFIAAIVCAFYTHAIVNPHLAAPVVGSSGAVAAVMGAYLVLRPTGRILTLIPLLP
                     SMDVVEVPSLIFILVWLALQILPAALTPHATSHVAWFTHIGGFLFGLVAGVNLRISGR
                     AKR"
     gene            3452..3670
                     /locus_tag="PLT69_06040"
     CDS             3452..3670
                     /locus_tag="PLT69_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOJ14034.1"
                     /translation="MREVGILLVVAGLAIAVFGLALAFGWGLGWIGRLPGDIVIERPT
                     MRLYFPVTTCILASIVLSVIVAILRWLK"
     gene            3679..>4066
                     /locus_tag="PLT69_06045"
     CDS             3679..>4066
                     /locus_tag="PLT69_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14035.1"
                     /translation="MAEAIVERVVARQASKSLRFTEGAPVEIPRPPSEGIHLYIHVPF
                     CMELCPYCSFHRFPFDGQAAVRYFAALRRELGLYARLGYSFSSVYVGGGTPTILMDEL
                     IATLECVHTLFGPSEISVETNPDRLDR"
BASE COUNT          714 a         1227 c         1285 g          840 t
ORIGIN      
        1 ggccaggtac ctcgctttct ccttgacgat ctccttcttt gccgcgagct cggccttcag
       61 ctggttcagc tccggctcaa ccgcggctat ctcggcctgg atgggtctga agaagaactg
      121 ccagtagacg cccacgacca gtcccagcac gaccgccagg acgaggacct tcacgagggg
      181 cttctggcgc aacagcgtgt ctatgatctt gtccatggtc gctgacctca tgacgagagc
      241 ttgatcttgc aggtaatgga gaacttctgc tggccgcctt cctgccttga caggacaagg
      301 ttcacgttgt cgaagtgctt ggatgcctgg agctgcttca agaacgccgc tatcgtcgtg
      361 ttgtcgaccg cggcgccctc gagaaccacg gtgtcgccct tgttcgtgaa ggaggtgagc
      421 cacgccttct cggggatgat catgctcagc tcgtcgagca cctcaaccgg cccggttctc
      481 ttcgcgttga ggtccctgat gacgtcgagc ttcttctcca gctcctgctt ctgcttcttg
      541 aacttctcga tctcgccggc ctctgcggtg agccgcttga tctcgacctt ggtgtcggcg
      601 atctccttct tcagggagtc cagcctggac accatggagt gctggacgaa atactcgctg
      661 atgaggacca gcgtgacgca cagcaagagg agcacgacgt gctcgatcag ggccctcttc
      721 ttcagctcga cacggatggg gaggaggttg atctttacca tttgtagtcg gccctcctta
      781 gtgcaaggcc cacagccact gccatctgcg gggcgatata ggccatgtac tcgggatcga
      841 atacgcgctc gtcccatgag atgttgttga aggggttcac tatctcgacg gggatctcca
      901 tctgcttctc gagcagcctc gtgatgcccg ggatcctcga ggaccctccg cttatgtaga
      961 tcttctcgat gttctcgttg accatggtcg acaggaagaa gtcgagggtc ctcctgatct
     1021 cctgcacgta ggtctcggcc gccatcttga agatgtagtt gatcttctcg aggtcgatcc
     1081 cctcgatgtc ggcccccgtc ttgatgttct cggcccgctc gaagctcacg ttgaactccc
     1141 gctggatgcg ctcggtgagc tggcgtccgc ccatggagat gtccctggcg aagatggaca
     1201 tgttgtcctt gaggacgttc acgttgatca tcgaggcccc gatgttcacg agagcgacga
     1261 tgctctcggg cgcatccggg tagttgacct cgtacatggt ctcgagggag aacacgtcga
     1321 tgtctatgag catgggcttg agccccgcgt tgcgcactgc ggaggcgtac tcgtcgacga
     1381 gctccttctt cgccgccacg aacagcacgt ccatcatgtc ggagcgctct atgctctccc
     1441 ccaggatgaa ggtgtccagg tagacgtcgt tgatgtcgaa ggggatgtac tgctcggcct
     1501 cgatgggcag gttctgcctg agctcggcct cgctcatgag cgccagctcg gccttcttca
     1561 cgatgaccga gtggcccgag atggccgtcg cggacccgcg cgtgcggacc ttcaggttct
     1621 ccctcaagtt cttgatggtg tccaccacgg tctcggaatc gatgatgtcc ttgtccacga
     1681 tgcactcggc agggatcaga ccgatgccca tcgacttgag ctgatagccg ctgcgagtct
     1741 tcgcgatatc caccatcttg atggactgtg aaccgatatc cagtcccagg agattcttct
     1801 cgagaaacat cgcctcaccc cgcgatcagt tctttttcct cttgaacacc acgatggtgt
     1861 ccccgggcct caccatgttc agttctctca cgatcacgtg ctctatatag gccatgttgt
     1921 ccctcaacat ggtggcctcc ttggcaagcg ccgcattctc ccgctcgagg tgttcgttgg
     1981 tgctcttgag agtggtgaga tctttgtgga gctggtatcc cctgatgacg ccgcgttccc
     2041 cggggagcgt gaagacgagc agtagggcga agacaagaat cgccccgagg ataagagcgt
     2101 gcgtcgttct catcgtctcc tgtatatccc aatacccatc ggactgcact aaaaatcaat
     2161 cccctttttc gcatagcctc tctatcggga agcggaaaat gaatctttag ggaaatatca
     2221 aattggtaac ggatgttgtc tttgtcaagg gaaagaaggc ctccgcgggt ttcactatcg
     2281 ttaccgcttc gccctgcccg agatcctgag attgacgccg gcgacgaggc cgaagaggaa
     2341 cccgcctatg tgcgtgaacc aggccacatg ggacgttgca tggggggtca gggcggcggg
     2401 caggatctgc agggcgagcc agacgaggat gaagatcagc gaggggacct cgaccacgtc
     2461 catcgagggc aaaaggggga tgagggtcag gatgcggcct gtcggcctga gcacgaggta
     2521 ggcgcccatg acggcggcga cggcgcccga cgagccgacc acgggggcgg ccaggtgagg
     2581 gttgacgatc gcgtgggtgt agaacgcgca gacgatcgcg gcgatgaaga acacggagaa
     2641 cgagacccat ccgaacgtcc tctccaccgc aggcgcgaag gcggagagga acacgaggtt
     2701 caaggcgagg tgaaggacgc cagtgtgcat gaagccggac aggatgatcg acgcatggag
     2761 gcgcaccagg ccgggcgacc ccaggacgag cgagttcctc gcatcgagcg ggctcaccct
     2821 cgagatggcc tggagcccgc cggagagcct gaggtccgcg atgtacaacg cgatggtggc
     2881 gaggatgagg agggtcgtga cgggcgcgaa ggcgcgctcg agggtcgtgt ccttcaccgg
     2941 gaacatgacg gcgtcacccc gtgcatgcga tcgccccctc gggccggttc tctcgggctc
     3001 ggtaccgctg cccgggcttc atctccggca acgaggccgt ggaagacccc cgacgcaggc
     3061 gcaggaagac gggagggggc gggcctggaa gcgccggatg gttccgtgac cctgatgggg
     3121 cgcatagcgt gcatgttcct gtggctcgcc ttaccatgaa cgtcccggtg cgggcaatcc
     3181 caaagaccct gtctggggca gggcgaaggc cgacgcgggt cctgtgccgg gtcttgtccg
     3241 cagaaacctg tggcccgggc cgtggaccgt ctgtgctgct tcctgccggg gggtgtcgcc
     3301 gcggggctcc tcgcctggac ctcggctgcc tccagcccct ttgcggccgg gggttgtacg
     3361 tcgtagcaga tgatcccact cgcataagaa gcccagcccc tttgcggacg ggggttgtac
     3421 cccgcagccc cctggtatat aggaaggtgc catgagggaa gtagggatcc tgctcgtggt
     3481 cgcggggctc gcgatcgccg tcttcgggct cgctctcgct ttcggctggg gtctcggttg
     3541 gatcgggagg ctgcctggcg acatcgtgat cgagaggccg acaatgcgcc tttacttccc
     3601 cgtcaccacg tgcatcctcg caagtatcgt cctcagcgtc atcgtggcga tcctgaggtg
     3661 gctcaagtga ggggccgggt ggcagaggcc atcgtcgaac gggtggtcgc ccgccaggcg
     3721 tcgaagagcc tccggttcac cgagggggcg cccgtggaga taccccgacc tccctcggag
     3781 gggatacacc tctacatcca cgtgccgttc tgcatggagc tctgcccgta ctgctccttc
     3841 caccgtttcc ccttcgacgg gcaggcggcg gtgcgatact tcgcggcgct caggagggag
     3901 ctcggtctct acgcgaggct cggctactcc ttctcgtcgg tctacgtggg cggcgggacg
     3961 cccacgatcc tcatggacga gctcatcgcg accctcgagt gcgtgcacac gctcttcggg
     4021 ccctcggaga tttccgtgga gacgaacccc gaccggctgg acagag
//
LOCUS       DAOSCR010000196         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13926_length_4066_cov_3.099227, whole genome shotgun sequence.
ACCESSION   DAOSCR010000196 DAOSCR010000000
VERSION     DAOSCR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13926_length_4066_cov_3.099227"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..451)
                     /locus_tag="PLT69_06050"
     CDS             complement(26..451)
                     /locus_tag="PLT69_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ14036.1"
                     /translation="MGGRLTKERLSLLKTVCNMKGHFQPAHVLEAMRSQGYGLSLATV
                     YRTLSLLAQAGIVTRVGASDDPDSAGSWYEHTWRREHHDHLVCTHCGAVIEFSYPAIE
                     VLQEAVAREHGFVLEGHSLELKGICARCRENITGGVGKT"
     gene            complement(837..1628)
                     /locus_tag="PLT69_06055"
     CDS             complement(837..1628)
                     /locus_tag="PLT69_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14037.1"
                     /translation="MCGTRKETLKKQRLHAVENHDIHTGDAGEMDCLVITYTFTMARG
                     DRASFRLEIDPVSIELRPQGGGPFPEWTRLTFKRCRVCTLTPSAHPFCPIAVRLVPIV
                     DRFKDVRSYEEVSLEVETAERTTLKVTSAQKALSSLMGLLCAVSGCPATRFLRPMARF
                     HLPLATQEETVYRAVSTYLLSRYFMVNNSTSNALAMNVSLDPLVRKYKALEEVNTGMS
                     ERIRSGARLDAAVNAMVILDSFAKALPFAVEDSLERLRPLFSTTP"
     gene            2341..2748
                     /locus_tag="PLT69_06060"
     CDS             2341..2748
                     /locus_tag="PLT69_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOJ14038.1"
                     /translation="MALFQWDDSYSVGIAVIDEQHRKLVGMINTLDDAMRQGRGKSVL
                     SGILSEMVRYTAMHFSTEEEYFKRYEYPGAGPHLEEHEKFTSKVREFQKGFDAGRIGL
                     STDVMNFLTDWLKKHILGSDKRYGQFLVERGVS"
     gene            2890..3708
                     /locus_tag="PLT69_06065"
     CDS             2890..3708
                     /locus_tag="PLT69_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ14039.1"
                     /translation="MRAHATSTAKGQDCISLKILSYNTHVGIRASRSSERLKNLWKHV
                     LPSRCKLDNLGKMAGFLSGFDLVGLQESDGGSLRSSFTNHTSLLASLAGFESWAEQIN
                     RDLFVARHSIGLLSRFPITKVVRHRLPGKIPGRGLLVVHIEAKGIPIAVAVGHLSLGS
                     KDRARQMIRIGEILGGIAERTIFMGDFNCTQESEEMRFLASMTGLRSALDHAPTYPSW
                     RPMRDIDHVMVSAGITIRRACTLSIPLSDHLPVAVEAVIEGVDGRLAGRTHLAA"
     gene            complement(3728..>4066)
                     /locus_tag="PLT69_06070"
     CDS             complement(3728..>4066)
                     /locus_tag="PLT69_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOJ14040.1"
                     /translation="PIDGICVRVGAMRCHSQGLTVKLAKDVPLGEINDIIAAANDWVT
                     VVPNTKEETLKNLTPAAVTGTLKVAVGRIRKMTLGERFLACFTVGDQLLWGAAEPIRR
                     MLRIALDHIG"
BASE COUNT          744 a         1222 c         1340 g          760 t
ORIGIN      
        1 gccggtcgtt ccccgtgttt ctttgtcatg tcttgccgac cccaccggtg atgttttccc
       61 tgcatctggc gcatattcct ttcagttcca gcgaatgtcc ctcgaggacg aacccgtgct
      121 ccctggccac cgcctcctgc aggacctcga tggctgggta cgagaactcg atgaccgccc
      181 cgcagtgcgt gcacacgagg tggtcatggt gctcccgacg ccaggtgtgc tcgtaccagg
      241 agccggccga gtccggatcg tcggaggcgc ccaccctggt cacgatgccg gcctgggcga
      301 gcaaggagag ggtccggtac acggtcgcca gcgataggcc gtacccctgg ctcctcatgg
      361 cctcgaggac gtgggcgggc tggaagtggc ccttcatatt gcaaaccgtt ttcaatagtg
      421 ataggcgttc tttcgtcagc cttcccccca tcgaggcaag gatatccttg aaattatcga
      481 aacatgtgcc cttgggggag cagtgtgaac agctgtcata ctgatcaata ttttgcatat
      541 tgttgtcaat agtaaacggg tgaacgcatg tcaagcgata tcgtgttcct gcgaagagga
      601 tcccaggggg gtgtacgagg taccgggggc ggcgcgcgat ggtttcgccg tctcttcgcg
      661 aggtgttcca ggaacaggtc tccggcgtaa gggccccgga aggcatacct ggctcaccca
      721 atcggtacgc gtcgcgtgcc gccggcgcct tgagatggtg acggagcacc cggtcggccg
      781 aatggcctcg agctctgtca tgacggcctt ggactgggcc cttgcctgca aggggctcag
      841 ggggttgtcg agaacagggg tctgaggcgc tccagggagt cttccacggc aaagggcagc
      901 gcctttgcga aggaatcgag gatcaccatg gcgttcaccg cggcatccag cctcgcgccc
      961 gagcggatcc tctcggacat gccggtgttc acttcttcca gtgccttgta tttcctgacg
     1021 aggggatcga gggacacatt catggcgagg gcattcgagg tggagttgtt gaccatgaag
     1081 tacctggaga ggaggtacgt ggagacagcc cggtacacgg tctcttcctg cgtggccaac
     1141 ggcaggtgga agcgggccat gggcctcagg aagcgggtcg ccggacaccc ggagacagcg
     1201 cagagcaggc ccatgaggga actgagggcc ttctgggcgc tcgtgacctt gagggtggtc
     1261 ctctccgccg tctccacctc caggctcacc tcctcgtacg atctgacgtc cttgaacctg
     1321 tccacgatcg gaacgagcct cactgcgatc gggcagaagg ggtgggccga gggcgtgagg
     1381 gtgcacacgc ggcatctctt gaaggtgagc ctggtccact ccgggaaggg gcccccgcct
     1441 tggggcctga gctctatgct cactgggtct atctcgagcc tgaaagacgc cctgtccccg
     1501 cgagccatgg tgaacgtgta cgtgatgacg agacagtcca tctcccctgc atctccggta
     1561 tggatgtcat ggttctctac ggcatgaagg cgctgtttct tgagggtttc cttgcgcgtg
     1621 ccgcacatgg ggaccccttt cctgcgttct gaagagacga catcgccggt gtgtcccgag
     1681 ccgttcgagc ggagggaagc agtaccatgg gccttggggg tgcggcggct ccttggggtg
     1741 cgggatgcaa gacggcaggc gcctgcgcga aggcccatgg cgtgcctcgt gacgggagtg
     1801 agtgcgggga aggagggcat cgatgaagag cgcgtctcga ccgggagaga cctcccactc
     1861 cgcgtgccat ccgggaaacc gggatatgta cgctgtcgcg tatcctggcc aaggggggtc
     1921 gagtacggtg cattccatga ggtgcccgaa cccggaggaa acccgcttga tgcacggatc
     1981 gagggaaagc catgcgcttt cttcccacca ggtgtctccc ggcgcctccc ctcgcacata
     2041 cgggtcccgg gccgtggatg cctcaaggtg aagggcctcc cccgtcgctg tggtgcgggg
     2101 ccgacacccc ggaggccggg gcatgacaag gcgggggtgg tgtcactccc tgccccggtg
     2161 tgatgtcggg taagcgcccc agcaagccct cccatgacgc gacgggtgaa agcctgcctc
     2221 gcatgcgcat ggtgtcgacc ccgtgcggac gacccataaa gtttcacgac ccctctccga
     2281 taatcacggg aagcaaccat gcaacgacag gtctcacaca gaccggagag gggggaatcc
     2341 atggcgctct tccagtggga cgactcctac agtgtcggca tagcggtgat agacgaacag
     2401 cacaggaagc tcgtgggcat gatcaacact ctcgacgacg cgatgcggca gggcaggggc
     2461 aagtccgtcc tgtccgggat actctcggag atggtccggt acaccgcgat gcacttctcg
     2521 accgaggagg agtacttcaa gaggtatgag tacccggggg ccgggccgca cctcgaggaa
     2581 cacgagaagt tcacgtcgaa ggtccgtgag ttccagaagg gcttcgacgc cggcaggata
     2641 ggcctgtcca cggatgtcat gaacttcctc accgactggc tcaagaaaca tattctgggt
     2701 tctgacaaga ggtacggcca gttcctcgtt gagagggggg tttcctgacg agacccgggg
     2761 ccagcctcca gggatgatcc acggttgcac gtcggcccaa tgtgtcggga ttcccgcgct
     2821 cgcgagccgt cgttgcattt cgttgaggag tgtgcaatac cagaccgaag cccgaagggg
     2881 ggacgacgag tgagggcgca tgccacatcg accgcgaaag gacaggactg catctctctc
     2941 aagatcctga gctacaacac ccacgtaggc atacgcgcct cccgttcgtc ggagaggctg
     3001 aagaacctgt ggaagcacgt gctcccctcg cggtgcaagc tcgacaacct cgggaagatg
     3061 gcgggctttc tatccgggtt tgacctcgtc gggctccagg agtcggacgg ggggagcctg
     3121 aggtcctctt tcacgaacca cacgagcctg ctcgcttccc tggcgggttt cgagagctgg
     3181 gccgagcaga taaaccgtga cctgttcgtc gcccgccaca gcatcgggct gctctccagg
     3241 ttccccataa cgaaggtcgt acgccacagg cttcccggga agatcccggg gcgcggtctc
     3301 ttggtcgtgc acatcgaggc gaaaggcata cccatagccg tcgccgtggg ccacctgtcg
     3361 ctggggtcga aagaccgcgc gaggcagatg atccgtatcg gggagatcct cggcgggatc
     3421 gccgagagga cgatcttcat gggggacttc aactgcaccc aggagagcga ggagatgcgt
     3481 ttcttggcct cgatgacagg gctcagaagc gccctcgatc atgccccgac ctacccgagc
     3541 tggaggccca tgcgcgatat cgaccacgtc atggtctcgg cggggatcac catccggcgg
     3601 gcatgcacgc tcagcatccc gctgtccgac catctccccg tggccgtcga ggcggtgatc
     3661 gagggggttg acggccgcct cgcaggcagg acgcacctgg ctgcttgagg cctgcaaccc
     3721 gggatcgtca cccgatgtgg tccaaggcga tcctgagcat cctgcgtatg ggctccgccg
     3781 caccccagag tagctgatcc cccacggtga agcacgcaag gaaacgttcg cccaaggtca
     3841 tcttccgtat gcgccccacc gcgaccttga gggtgccggt gacggcggcg ggggtgaggt
     3901 tcttgagcgt ctcctccttg gtgttcggta caacggtgac ccagtcgttc gcggcggcga
     3961 tgatgtcgtt gatctccccg agggggacgt ccttcgccag cttgacggtc aagccttggg
     4021 agtggcagcg catggcgccc acgcgaacgc agatgccgtc tatggg
//
LOCUS       DAOSCR010000197         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13936_length_4064_cov_4.484161, whole genome shotgun sequence.
ACCESSION   DAOSCR010000197 DAOSCR010000000
VERSION     DAOSCR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13936_length_4064_cov_4.484161"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..768
                     /gene="cmk"
                     /locus_tag="PLT69_06075"
     CDS             106..768
                     /gene="cmk"
                     /locus_tag="PLT69_06075"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ14041.1"
                     /translation="MKHRIITIDGPAGSGKSTVAREVARRLGFTYLDTGALYRAAALA
                     VISSGVEARDGVACASVIDKAEISLHEDKVYLDGRDVSSDIRSPGVAEAASAIAVHAD
                     VRKRLTALQRELADRESIVAEGRDTGSVVFPGADLKIFLDADIAERARRRHAEFARKG
                     MDADPSEVFESMRRRDEQDGSRRHSPLRVPENAFVVDTTHLTQEEVVVKILSMVRQAL
                     AD"
     gene            complement(757..981)
                     /locus_tag="PLT69_06080"
     CDS             complement(757..981)
                     /locus_tag="PLT69_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14042.1"
                     /translation="MNRKGNLDIILKNMDTSRATKVLAKSLYKELVRNGFTHKDIIDF
                     SREMIDYVAHEMRREATMEEAGRKDRLLIG"
     gene            complement(1240..2499)
                     /gene="pcnB"
                     /locus_tag="PLT69_06085"
     CDS             complement(1240..2499)
                     /gene="pcnB"
                     /locus_tag="PLT69_06085"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOJ14043.1"
                     /translation="MEETTSPVILDRSAHPISRRDIDPDALKVLYRLSRHGFKAYLVG
                     GAVRDLLLGKKPKDFDVATDARPGQVKKLFSNCFLVGRRFRLAHIRFRGNKIIEVATF
                     RRDPDPEHDSPTDIHNTFGTPVQDALRRDITMNALFYDIETFSVIDFVGGLEDIANRR
                     VRVIGDPPGRFSEDPVRMWRVLRHASRLGFAVDEHAARAIRDQRGLLAGCSGARLYEE
                     LNKDLSYGKARETFSLLMEYGLLSILLGAVGERLEQDASLAGAFLDLMGAHDRMLASG
                     GKFEATVAYSLLLWPWACATLMSGSAPGTDRARLLAEEIERAGMIVTIPKGLRSSAVH
                     VILAVNAMMRSLETGRLKTSLMGRSDYGTALRVFALVSGIPVSDGKDPFLELFRARYP
                     GVRERRARKRRRRRRRRPQGNEPSDTA"
     gene            complement(2468..2650)
                     /gene="yacG"
                     /locus_tag="PLT69_06090"
     CDS             complement(2468..2650)
                     /gene="yacG"
                     /locus_tag="PLT69_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002720789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOJ14044.1"
                     /translation="MKCPGCGREVPYEGNPYRPFCSRGCKGLDLVHWVSGEYRIPSKP
                     EEESPRDGRDDEPSHS"
     gene            2876..3532
                     /locus_tag="PLT69_06095"
     CDS             2876..3532
                     /locus_tag="PLT69_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ14045.1"
                     /translation="MQKKIRRERQIQRNRTTILKSARELFHKRGFYGTSMEDIAKHSG
                     FDRRTIYNHFKNKEDIFAALISGILSDIEAVFGEVASEEVSSLERLRILVLKLLDLYI
                     DNAHLLNIFMSEYETNEIKKRRHLSAYTLKNISAYRKIESKLVDMIRQAQVDGFVVDV
                     HPYILAGMLNEIILRSVIVMLHQRDSIKKKDIMGDIFELLSKNVVRTPREEPGSEKRP
                     "
     gene            complement(3859..>4064)
                     /gene="groEL"
                     /locus_tag="PLT69_06100"
     CDS             complement(3859..>4064)
                     /gene="groEL"
                     /locus_tag="PLT69_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164361.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ14046.1"
                     /translation="ADTEVYEDLVKAGVIDPTKVTRSALQNAASVAGLLLTTEVMVAE
                     IPEEKPAQGMPGGGGMPDMGGMM"
BASE COUNT          778 a         1282 c         1223 g          781 t
ORIGIN      
        1 gatgattccg gtggtcaagg gctggagcac cgagatcggc atcgacgacc cctcctgcgt
       61 caggatatca tacccgaggt tcttcgagga catggactcc ctcgcatgaa acaccgcatc
      121 atcaccatcg acggccctgc aggaagcgga aaatcgacgg tcgcccgcga ggtcgcccgc
      181 cggctgggct tcacctacct ggacaccggc gccttgtacc gggccgccgc actggccgtg
      241 atctcgtcgg gggtcgaagc cagagacggt gtcgcatgcg cctcggtgat cgacaaggcc
      301 gagatatccc tgcatgagga caaggtgtac ctggacggga gagacgtgag ctccgacata
      361 aggtcgcccg gcgtcgccga ggcggcctcc gccatagccg tccatgccga cgtgaggaaa
      421 cgcctcaccg ccctgcagag ggagttggcc gaccgcgaat ccatcgtcgc ggagggccgg
      481 gacacgggct cggtggtctt ccccggcgcg gatctcaaga tcttcctcga cgcggacata
      541 gccgagcgcg cccgccgcag gcatgcggag ttcgcccgga aggggatgga tgcagacccg
      601 tcggaggtct tcgaatccat gagaagacgg gacgaacaag acgggtcgag gcgccactcg
      661 cctctcaggg tgccggaaaa cgcgtttgtg gtggacacga cacacctcac gcaggaagag
      721 gtggtggtca agatactctc gatggtaaga caggcgttag ccgattagga ggcggtcctt
      781 gcggccggct tcctccatcg tggcctccct gcgcatctca tgggccacgt agtcgatcat
      841 ctcgcgcgag aagtcaatga tgtctttatg ggtgaacccg ttgcggacaa gttccttgta
      901 caggctcttg gcaagaacct tggtcgccct cgaagtatcc atgttcttca gaatgatgtc
      961 gagattccct ttcctgttca tgtccattcc ccgtcttgtt cccgcttttt atgcatctac
     1021 cgtgccaaag aggagggatg ggatttttga cgtgattcat tgctgttagg agcgggcagg
     1081 gtcacccctg gggaacactc tgtgggaaat atgcaatagc cactgaatca cggatcagtg
     1141 cgggagggaa tatctccttt aataccgtga tgttgcatgc atggccctca gaccacacga
     1201 accgtgtacc tcgtgcattc cccgttcagg gccgccaggt cacgccgtgt cgctcggctc
     1261 gttcccctgg ggacgcctgc gacggcgcct cctcctcttt cgggcgcgcc tctctctcac
     1321 gcccgggtag cgcgcgcgaa agagctcgag gaacgggtct ttcccatcgg acacggggat
     1381 acctgacacg agggcgaaca ccctgagggc ggtcccgtag tcggagcggc ccatgagcga
     1441 ggtcttgagc cttcccgtct cgagggaccg catcatggcg ttcaccgcga gtatgacgtg
     1501 gacggcgctg gagcgcagcc ccttggggat cgtcacgatc atgccggcgc gctcgatctc
     1561 ctcagcgagg agcctcgccc tgtccgtgcc gggggccgaa cccgacatca aggtagcaca
     1621 cgcccagggc cacaggagga ggctgtatgc cacggtcgcc tcgaactttc cgccagaggc
     1681 gagcatcctg tcatgcgccc ccatgagatc aagaaaggcg ccggcaagcg atgcgtcctg
     1741 ttccagccgt tccccgacgg cgccgagaag gatggagagg aggccgtatt ccatgaggag
     1801 cgaaaaggtc tcccgggcct tgccgtagct caagtccttg ttgagttcct cgtagagtct
     1861 cgcgcccgag catccggcga gcaacccgcg ctgatcgcgg atggcccgtg cggcatgctc
     1921 gtccactgcg aacccgagcc tcgaggcgtg tctcaacacc cgccacatgc gcaccgggtc
     1981 ctccgagaaa cggccgggcg ggtccccgat gaccctcact ctgcggttcg cgatgtcctc
     2041 gagcccgccc acgaagtcga tcaccgagaa ggtctcgatg tcgtagaaca gggcgttcat
     2101 ggtgatgtcc ctcctgaggg cgtcctggac aggcgtgccg aaggtgttgt ggatgtcggt
     2161 cggggagtcg tgctcagggt cggggtccct cctgaacgtg gccacctcga tgatcttgtt
     2221 tcccctgaac ctgatgtgcg ccagcctgaa tctccggccc acgaggaagc agttggagaa
     2281 gagtttcttg acctgccccg gccgcgcgtc cgtggcgacg tcgaaatcct tcggcttctt
     2341 gccgaggagg aggtccctca cggctccgcc gacgagatag gccttgaacc cgtgcctgct
     2401 caggcggtag aggaccttca gcgcgtcggg gtcgatatcc cttcgggaga tggggtgtgc
     2461 cgaccggtca agaatgactg ggctcgtcgt ctcttccatc tctcggcgac tcttcctcgg
     2521 gtttcgaggg gatcctgtat tccccgctca cccagtggac aaggtccagg cccttgcatc
     2581 ccctcgaaca gaaaggccgg tacggattcc cctcgtaggg cacttccctg ccgcagccag
     2641 gacacttcac gacccttacc ggcggtctcg accccctgtc gttcccccct gacgtcacgc
     2701 tcgaacctcc cgtatttccg gaatcttctc ataccccatt tcgcgtacgt tgtacatcgg
     2761 ggcattcttt ctccgcagct tgtgaattgt cggcctgttt catgagggat acttttctat
     2821 tgacttgaaa gagagaagag tgatttggtg atcgcgggcc tcaccgggac gagccatgca
     2881 gaagaagatc aggcgagaga gacagatcca gcgaaacagg acgacgatcc tcaagtccgc
     2941 ccgcgagctc ttccacaaga ggggcttcta cggaacctcc atggaggata tcgccaagca
     3001 ctcggggttc gacaggcgga ccatctacaa tcacttcaag aacaaggaag acatcttcgc
     3061 cgctctcatc tcgggcatac tctcggacat cgaggcggtc ttcggcgagg tcgcatccga
     3121 ggaggtctcg tcgctggaga ggctgagaat cctcgtgctc aagctcctcg atctctacat
     3181 cgacaacgcc cacctcctca acatcttcat gtcggagtac gagacgaacg agatcaagaa
     3241 gagacgccac ctctcggcct acaccctcaa gaacatcagc gcctacagga agatcgaatc
     3301 caagctggtg gacatgatca ggcaggccca ggtcgacggc ttcgtcgtgg acgtgcatcc
     3361 ctacatcctc gccggcatgc tcaacgagat catcctgcga agcgtcatcg tgatgctcca
     3421 ccagagggac tccatcaaga aaaaggacat catgggcgac atcttcgagc tcctctccaa
     3481 gaacgtcgtc aggacaccga gggaagagcc cggttccgag aagaggccgt agcttccgcc
     3541 agaaacagga tttccctccg tgccgacggc acatggatga gacctctaca tcgctctgcc
     3601 tccagggtgt tccgctcggc tgccgcttat cccccgtcgc ccgacgggaa gccgtctcgc
     3661 cagttgggac tccccctcac gggacacgag cggcagcgtc cgggtcacag ccatggaaac
     3721 gtatcgcacg agagaccccc ggtcttcgag tacaggccgc aggtacgcgg gacttcccat
     3781 gaagatggag acgggggagg caagatgctt cccccgtctt tgttcgtgcg tgcagggtat
     3841 ggggtgttca gcgacggctt acatcatgcc gcccatatcc ggcattcctc cgccgccggg
     3901 cataccctga gccggtttct cctccgggat ctcggcgacc atgacctcgg tggtgagcaa
     3961 gagcccggcc acggatgccg cgttctggag cgcggagcgc gtcaccttgg tggggtctat
     4021 gacgcccgcc ttcacgaggt cctcgtacac ctcggtatcc gcgt
//
LOCUS       DAOSCR010000198         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_13964_length_4059_cov_3.062687, whole genome shotgun sequence.
ACCESSION   DAOSCR010000198 DAOSCR010000000
VERSION     DAOSCR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13964_length_4059_cov_3.062687"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLT69_06105"
     CDS             complement(<1..260)
                     /locus_tag="PLT69_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOJ14047.1"
                     /translation="MSESQWFVVQTNPREEQLASTVLTQHGVAVYQPYMEKFVFHARK
                     KTLKRYPLFPGYLFVHIRPVEEDFHKVRWSRGVRKILIDNYQL"
     gene            complement(791..1957)
                     /locus_tag="PLT69_06110"
     CDS             complement(791..1957)
                     /locus_tag="PLT69_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOJ14048.1"
                     /translation="MRRRRKPRRKPPKTVVVTAVVGVLIALILVISAVMIYTERRQGP
                     HQAGQPAPSSERTRVVSTTRIAAEELLVLGCLYELGIPRENVGTKGRTVHVRVTKPPS
                     QSRIKKAFAPLEELEGVSVRMEHPGLLVITMNGHTWDVIFEGAAPMSKTRARVAIIVD
                     DIGQEMAPVRRLAAIDADLTFSVLPNLPYTQDAARYLHRRGREILLHQPMEGKNGVNP
                     GPGAIYASTDPAEAASILEDSISQVPYAVGVNNHMGSAVTRIRPTMDALFDVLGKRRL
                     FFVDSLTTNESVCRQAAAAAGIPFASRDVFLDNVQDDAYIAGQIKKLEEEALVKGSAI
                     GICHPHEATVRTLEREVPRLVEEGIEVVRVSVLAESPNGWAGEKAPVRHGAASW"
     gene            complement(1954..3243)
                     /locus_tag="PLT69_06115"
     CDS             complement(1954..3243)
                     /locus_tag="PLT69_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ14049.1"
                     /translation="MKRHRAVLPGLVAAGLVLLVALHTIPHARAESSPMLPSLQKFTD
                     CLDLIERYYVDKVEPGKLIEGAIKGMTSTLDPHSAYLSKEDYKEMEISTTGSFGGLGI
                     ELGIKDGQLTVITPIEDTPAFKAGIKPGDKILKIDGQSTTDMTLEEAVKLLRGPKGTK
                     VTLTLGRESLDKPLELTITRATIHIESVVSQTLEPGYGYVKVRSFQVDTADAVKKAVQ
                     KLLKAGGLQGLVLDLRYNPGGLLDQAIAVSDLFLEAGKIVYTDGRNPEDHVEYRAHKD
                     GTFEYFPMVVLINGGTASAAEIVAGALQDNERAVIVGVKSFGKASVQTIRQLGDGSAL
                     KLTVARYYTPSGRSIQAKGIEPDVFVEQAVLLPEKGQSFIREKDLKSHLEPPDAEKPE
                     AAQAPDGKARPENDYQLKYGLDLLKTWGVFNRAKKAT"
BASE COUNT          554 a         1348 c         1415 g          742 t
ORIGIN      
        1 agctggtagt tgtcgatgag gatcttcctc acgcccctgc tccagcggac cttgtggaag
       61 tcctcctcga cgggccggat gtgcacgaag aggtagccgg gaaagagcgg gtagcgcttg
      121 agggtcttct tcctcgcgtg gaacacgaac ttctccatgt acggctggta cacggcgacc
      181 ccgtgctggg tgagcaccgt tgacgcaagc tgctcctccc tggggttcgt ctgcaccaca
      241 aaccactgcg actcgctcac gcggctacct atactgcagt cgcggccggg cagtcaatcc
      301 gccgacaacg gtggggccga gcgggccgtc cgtccccctt tcccaggagg gcgcggcccg
      361 ccgcagggaa ggaaagggtc gcacggtcac cgtgcaggcg cacgcaatga tggcgtcgcc
      421 gaccatcgtg ggcaggagtg gacggaaagg ggttcgcggt cgccgtggcc ggcgcacccc
      481 ggggggtggc gtcacgatgc gtgcgctacc cgcgaggccg tgggcggcgc gaagggaggg
      541 catgcaaggg aatccatggt ctctcgagcg ctacccggcc ggccgcatcc ggcgccgggc
      601 gttgccggct ccccgggggg gcggtcatgg ggggtacaca gcccttgcac gggcgggcgg
      661 cagtccggcg ctcgttcgcg cgccgggccg gaggcggggc ctcgcccggg gtggccatgc
      721 cccgggaaac cccgtcggcg tcaggtccgc cccgtcgtcc gggcccgtcc ccgtcgccgg
      781 tctccctgcg tcaccatgac gcagcgccgt gacgaacagg ggccttctcg cccgcccacc
      841 cgttcgggct ttcggccagc accgagaccc tcaccacctc gatgccctcc tcgacgaggc
      901 gcgggacctc ccgctcgagg gtcctgacgg tcgcctcgtg ggggtggcat atgccgatgg
      961 cgctgccctt gacgagcgcc tcttcctcga gcttcttgat ctggcccgcg atgtaggcgt
     1021 cgtcctggac gttgtcgagg aacacgtccc tcgatgcgaa gggtatgccg gctgccgcgg
     1081 cggcctgacg acacacggac tcgttcgtcg tgaggctgtc cacgaagaag aggcgccgct
     1141 tgcccaggac gtcgaagagg gcgtccatgg tgggcctgat cctcgtgacc gccgacccca
     1201 tgtggttgtt cacgcccacg gcgtaaggca cctgggagat ggagtcctcg aggatggatg
     1261 cggcctcggc gggatcggtg gaggcgtaga tggccccggg gccggggttc acgccgttct
     1321 tgccctccat gggctggtgg aggaggatct ccctgccgcg cctgtggagg tagcgggccg
     1381 cgtcctgcgt gtaggggagg ttgggcagca cgctgaaggt gaggtcggcg tcgatggccg
     1441 cgagcctgcg caccggcgcc atctcctggc cgatgtcgtc gacgatgatc gcgacgcgcg
     1501 cccgggtctt gctcatcggg gccgccccct cgaagatcac gtcccaggtg tggccgttca
     1561 tggtgatcac gaggagcccg gggtgttcca tgcggacgga caccccctcc agctcctcca
     1621 ggggggcgaa cgccttcttg atgcgggact gggagggcgg cttcgtgacc cgcacgtgca
     1681 ctgtcctgcc cttggtgccc acgttctccc tggggatgcc cagctcgtag aggcagccca
     1741 ggacgaggag ctcctcggcc gctatcctgg tcgtggagac aaccctcgtc ctctccgacg
     1801 agggagccgg ctgtcctgcc tggtgtgggc cctggcgtct ctcggtgtag atcatcaccg
     1861 ccgagatcac gaggatgagg gctatgagca cccccacgac cgccgtcacg acgacggtct
     1921 tcggaggttt cctccggggc tttcgtcgcc ttctcatgtc gccttcttgg cccggttgaa
     1981 caccccccac gtcttgagca ggtcgaggcc gtacttgagc tggtagtcgt tctcgggcct
     2041 cgccttgccg tccggggcct gggcggcctc gggtttctcg gcgtcgggcg gctcgaggtg
     2101 ggatttcagg tccttctccc gtatgaagct ctggcccttc tccggcaaga ggacggcctg
     2161 ttcgacgaag acgtccggct cgatgccctt ggcctggatc gagcggcccg agggcgtgta
     2221 gtacctggcc acggtgagct tgagcgccga gccgtcaccg agctggcgga tggtctggac
     2281 ggaggccttg ccgaagctct tcacccccac gatgacggcc ctctcgttgt cctggagcgc
     2341 acctgcgacg atctcggccg cgctcgccgt accgccgttg atgagcacga ccatagggaa
     2401 gtactcgaac gtgccgtcct tgtgcgccct gtactcgacg tggtcttcag ggttcctgcc
     2461 gtcggtgtag acgatcttgc ccgcctccag gaagaggtcg ctcaccgcga tggcctggtc
     2521 caagagacct cccgggttgt acctgaggtc gaggacgagc ccctgcaggc ccccggcctt
     2581 gagaagcttc tggacggcct tcttgaccgc gtccgccgtg tcgacctgga agctgcggac
     2641 cttgacgtaa ccgtagcccg gctccagggt ttgggacacg acgctctcga tgtggatggt
     2701 ggcccgggtg atggtgagct cgagaggctt gtccagggat tcccggccca gggtgagcgt
     2761 caccttcgtc cccttgggac ccctcagcag cttcactgcc tcctcgaggg tcatgtccgt
     2821 ggtggactgt ccgtcgatct tgaggatctt gtcgccgggc ttgatgcccg ccttgaaggc
     2881 cggggtgtcc tcgatggggg tgatgaccgt gagctggccg tccttgatgc ccagctcgat
     2941 gcccaggccc ccgaacgacc cggtggtgct gatctccatc tccttgtaat cctccttgga
     3001 gaggtacgcg gagtgggggt cgagcgtgga ggtcatgccc ttgatcgcgc cctcgatgag
     3061 tttccccggc tccaccttgt ccacgtagta ccgttcgatg aggtcgaggc agtccgtgaa
     3121 tttctggagg ctcggcagca tgggcgagct ctccgccctg gcgtgcggga tcgtgtgcag
     3181 cgcgacgagg agtacgagcc ctgccgctac gaggccgggc aggacggccc tgtgtctttt
     3241 catgggttcc tttcccctgg ggcgtgtggt gtcgatgacc gacctatagc acaactcatg
     3301 cggccggaaa acacctgcgg ttcgtccctg ttcccccggt cggggccctt gcccgaggcg
     3361 ccctcggtca cccccgccga gggccgggag gctgatgaaa ccccgggtgc cgggccctcg
     3421 ggcgggaggg ccccgcgtcc ttgggccgtg ggacacgggc ccccgctcgt gtggagacgt
     3481 agcgcatcgt tcgtgcgcag ggccatctcc tggtccacgg tccacagggc cccgctagcg
     3541 tggagacgcc ttgtatgtcg tgggcggtgt tacggctatc aaccacaagc ctcgatctgt
     3601 ggagacgccg aaaccacggc cacgggcctc ggggatcaga acgcccgcat gcccccgctc
     3661 gtgtggagac gccgccccat cgcggcgatt acgacctctt ctgggacgca tgcccccgct
     3721 cgtggggacg cccctggacc aggacccggc ggggagaggt cgctctgttc ggccgcgagg
     3781 cgccggcccg gagccgaccc tcgtgtcatc gccagggtgg agaccctcgc gggggatgtc
     3841 gtgtgcggcc cgtgcctgcg cagcgggagg agggctcgac gggacgggcg gggtcgcggt
     3901 catgaaaccg cccgggttct caccgcttgg aggagagcca cctgacaggg tcctggggct
     3961 tgtccttgta cctgatctcg aagtggaggg tgggcctgga aacgtcgccg ctcgcgccca
     4021 cgagcgccac ctcctcctgc gccttgacct cttcccccg
//
LOCUS       DAOSCR010000199         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14028_length_4044_cov_3.496616, whole genome shotgun sequence.
ACCESSION   DAOSCR010000199 DAOSCR010000000
VERSION     DAOSCR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14028_length_4044_cov_3.496616"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PLT69_06120"
     CDS             <1..1759
                     /locus_tag="PLT69_06120"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ14050.1"
                     /translation="SNVSGAPIRERSFIIDKTMRDPILQSFLESLAQGDAKALKAAEH
                     TARKYLDQIASDLTPSTITFLDKSLTWVFNNIFEGLDVDMAGLQAVRDRARTGSLVYV
                     PCHKSHIDYLILSYCLYHNWMSVPLIAAGINLSFFPIGSILRKGGAFFMRRTFKDNPL
                     YAQTFSAYVRTILGERIPMEFFIEGTRSRSGKLMLPKRGLLSMIIQGWEAGVCRDVIF
                     VPVYVGYDTVVEENAYIREMKGAPKERESFLQLLKAGSILKNRYGKVYIRFAPPLSLE
                     EYMMSRPGASGMSPDQKEELYEDVATRIISAIYRQTVVTPVSLMSCVLLSLGRALDEA
                     ELKEGFHVFLEYLKFQGCSLSSTLANEDSAYKEAIELLEGKGLLTLDAGESNSEPGLA
                     YVENEDRIHLEYYKNTILNFFVPVSLIANVLLKNKGSTVEKDLQRQVRKLAGLLEKEF
                     ILDLESFYKALDFLTSQGIVTVSKGVYTASERSVDVLRLFGGLVENYLESYLCVAENL
                     DRIQDTPEKNVLKAINRHAERMFKKGEIKRFESLCLPVYKGALDTFREKGLIDAKNRI
                     TDRKRIEELVHEIRELLET"
     gene            1731..2366
                     /locus_tag="PLT69_06125"
     CDS             1731..2366
                     /locus_tag="PLT69_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOJ14051.1"
                     /translation="MKSGNCSRRKLAVSLFVLFLATGCAHQYRPGPGWVEVGVASWYG
                     EEFHGRPTACGEPYNMYALSAAHKTIPLGSKVRVTNLDNGRQIVVPVNDRGPFVGDRI
                     IDMSYGAARVLDMVECGLARVRVEVLEMPRYYTGGRYTIQFGAFAVKENAERLASVIE
                     SKGYQPSVEQAQVYGNTVYRVRLGAFSSMEGAKDLAQSFASQGISCYIVGL"
     gene            2411..3700
                     /gene="eno"
                     /locus_tag="PLT69_06130"
     CDS             2411..3700
                     /gene="eno"
                     /locus_tag="PLT69_06130"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ14052.1"
                     /translation="MSEIVDVVAREILDSRGNPTVEVDVFLSTGDMGRAAVPSGASTG
                     ANEAIELRDGDPKRYKGKGTTKAVDNVNKKISSALVGMDALDQVGIDRMLNELDETPN
                     KSNLGANATTGVSVAVAKAAANFLGLPLYRYLGGAFAREIPVPLLNVVNGGKHADNNV
                     DLQEFMIVPLGAPNFREALRYAAETFHALRDILVASGYSTGVGDEGGFAPNLKSNAEP
                     LELIVKAIEKAGYRPGEDVAIALDPAATSFYKDGKYILASERDPERTSEEMIDFYEDL
                     IRRFPIVSIEDGLAEDDWEGWKKLMVRLGGRIQIVGDDLYVTNKEYLARGIAEGTSNS
                     ILIKVNQIGTLTETIETIEMARRAGFTTVFSHRSGETEDAWLADVAVATNAGQLKTGS
                     TSRSERLAKYNQLLRIEEELGAAAVFRGKGVLYSIRK"
BASE COUNT          846 a         1266 c         1218 g          714 t
ORIGIN      
        1 cagcaacgtc tccggcgcac ccatccgcga gcgttccttc atcatagaca agaccatgcg
       61 ggacccgatc ctccagtcct tcctggaatc cctcgcccaa ggcgacgcca aggccctcaa
      121 ggcagccgag cacacggcga ggaaatacct ggaccagata gcatccgacc tgacgccatc
      181 cacgatcacc ttcctcgaca aatcgttgac ttgggtgttc aacaacatct tcgagggtct
      241 cgacgttgac atggcgggtc tccaggcggt gcgcgacagg gcgaggacag gctcgctcgt
      301 ctacgtcccc tgccacaaga gccacatcga ctacctgatc ctctcgtatt gcctctacca
      361 caactggatg agcgtccccc tgatagccgc gggcatcaac ctctccttct ttcccatagg
      421 ctcgatcctg agaaagggcg gggccttctt catgcgccgg accttcaagg acaaccccct
      481 gtatgcccag accttcagcg cgtacgtcag gaccatcctc ggggagcgga tccccatgga
      541 gttcttcatc gagggcacga ggagccgctc ggggaagctc atgctcccca agaggggtct
      601 tctgtccatg atcatccagg ggtgggaggc cggcgtgtgc agggacgtca tcttcgtccc
      661 cgtctatgtc ggatacgata ccgtggtgga ggagaacgcc tatatccgcg agatgaaggg
      721 cgccccgaag gaaagggaga gcttcctcca gctcctcaag gccggctcca tcctgaagaa
      781 ccgctacggc aaggtgtaca tcaggttcgc ccctcccctg tccctcgagg agtacatgat
      841 gtcgaggccg ggcgcctccg gcatgagccc ggaccagaaa gaggaactct acgaagacgt
      901 ggcgacgagg atcatctccg ccatataccg ccagaccgtc gtcaccccgg tatccctgat
      961 gtcctgcgtg ctcctgagcc tcggccgggc tctcgacgag gccgaattga aagaaggctt
     1021 ccacgtcttt cttgaatacc tgaagttcca ggggtgttcg ctctcctcga cgctcgccaa
     1081 cgaggactcc gcctacaagg aagccatcga actcctcgag ggcaagggcc ttctgaccct
     1141 ggatgcaggt gaatccaact ccgagccggg gctcgcgtac gtggagaacg aggacaggat
     1201 ccacctcgag tactacaaga acaccatcct caacttcttc gtgcctgtct ccctcatagc
     1261 gaacgtgctc ctcaagaaca agggctcgac tgtcgaaaag gaccttcaga gacaggtcag
     1321 gaagcttgcg ggcctcctgg aaaaggagtt catccttgac ctggagagct tctacaaggc
     1381 cctggatttc ctgacgtcgc aagggattgt gacggtgagc aagggagtgt acacggcatc
     1441 cgagagatcc gtcgatgtcc tgagactgtt cggtggactc gtcgagaact acctcgagtc
     1501 gtatctctgc gtggccgaaa acctcgacag gatacaagac accccggaga agaacgtcct
     1561 caaggccatc aaccgccacg ccgagcggat gttcaagaag ggggagatca agcgattcga
     1621 gtcgctgtgc ctgcccgtgt acaagggcgc cctcgacacc ttcagggaaa agggtctcat
     1681 agacgccaag aaccgcatca cagacaggaa acgcatcgag gagctcgttc atgaaatcag
     1741 ggaattgctc gagacgtaaa ctcgccgtat cgctgttcgt cctttttctc gcaacggggt
     1801 gcgcccacca gtacaggccc ggtcccggct gggtggaggt cggtgtcgcc tcgtggtacg
     1861 gcgaggagtt ccacggcagg ccgacggcgt gcggcgagcc ctacaacatg tacgcgctga
     1921 gcgccgcgca caagaccatc ccgctcggga gcaaggtgag ggtcacgaac ctcgacaacg
     1981 gcaggcagat cgtcgtcccg gtcaatgacc gagggccctt cgtgggcgac aggatcatcg
     2041 acatgtccta cggcgccgca agggttctcg acatggtgga atgcggcctc gcccgggtcc
     2101 gcgtagaggt cctcgaaatg cccaggtact acaccggggg caggtacacg atccagttcg
     2161 gggccttcgc ggtgaaggaa aacgcggaga ggctggcctc cgtcatcgag tcgaaggggt
     2221 accagccgag cgtcgagcag gcccaggtct atggtaacac cgtgtaccgt gtcaggctcg
     2281 gcgcgttctc ctccatggag ggggcgaaag acctcgcaca gtcgtttgca tcccagggta
     2341 tctcctgcta tattgtgggt ctctgaacca ggcgacaaga tacccaccat atgaccgagg
     2401 aggagaggcc atgagcgaaa ttgtcgatgt tgttgcgcgc gagatcctgg attcgagggg
     2461 caatcccacc gtagaggtcg acgtgttcct ctcgacgggg gacatgggca gggcggccgt
     2521 cccgtcaggt gcgtccaccg gtgcgaacga ggccatagag ttgagggacg gcgatcccaa
     2581 gagatacaag ggcaaaggca ccacgaaggc ggtggacaac gtgaacaaga agatctcttc
     2641 cgcgctcgtg ggcatggacg ccctcgacca ggtcggcatc gaccgcatgc tcaacgagct
     2701 cgacgagacg ccaaacaagt cgaacctcgg ggcgaacgcc acgacgggcg tctccgtcgc
     2761 ggtcgcgaag gcggctgcga acttcctcgg cctgcccctc tacaggtacc tgggaggcgc
     2821 tttcgcacgg gagatcccgg ttcccctgct gaacgtcgtc aacgggggca agcacgccga
     2881 caacaacgtg gatctccagg agttcatgat cgtgccgctg ggcgcaccga acttccgaga
     2941 ggcactgaga tacgcggccg agaccttcca cgccctccgc gacatcctgg tcgcgagcgg
     3001 ctattccacg ggggtcggcg acgagggggg cttcgcgccg aacctcaagt cgaacgccga
     3061 acctctcgag ctcatcgtga aggccataga gaaggccggc tacaggcctg gtgaggacgt
     3121 ggccatcgcc ctcgaccctg ctgccacctc cttctacaag gacggcaagt acatactcgc
     3181 ttccgagagg gacccggaga ggacctcgga agagatgata gacttctacg aggacctcat
     3241 caggaggttc cccatcgtct ccatcgagga cggtctggcc gaggacgact gggagggttg
     3301 gaagaaactc atggtgcgcc tcggcgggag gatccagatc gtcggggacg acctctacgt
     3361 caccaacaag gagtacctcg caagggggat cgccgagggg acatccaact ccatcctcat
     3421 caaggtgaac cagataggga ccctcacgga gaccatcgaa acgatcgaga tggcgcgcag
     3481 ggccggtttc acgacggtat tctcccaccg ctccggcgag accgaggacg catggctcgc
     3541 cgacgtggcc gtggcgacga acgcaggcca gttgaagacg gggtccactt cccgcagcga
     3601 gaggctcgcg aagtacaacc agctcctgcg catcgaggaa gagctcggtg cggctgcggt
     3661 cttcaggggc aagggcgtgc tctacagcat ccggaagtaa agccaatgaa gccgggcggc
     3721 gtctcgacca ggtgagacgc cgccttctct ctctcccctt ccagcctcct ccatcgtgtg
     3781 tgccatcggc atcacccagc gcaggccgga ggccgcctcc tttctctcgt tttcgcctct
     3841 tcccctgccc tctctccttc gaccacgcca ggctcgcacc ccaaacgccc acctctctcg
     3901 ggcgagcgcc gtcggccttc ctgttgcttt ttcctctgtc ttgttctagg gttcactctc
     3961 gtgcgtgcgc tggaacgtgg acggtcaccg cgcgcatggc gatgatacgc tgagataaga
     4021 ggagggatca tgcaagaggt cgtc
//
LOCUS       DAOSCR010000200         4039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14051_length_4039_cov_3.311747, whole genome shotgun sequence.
ACCESSION   DAOSCR010000200 DAOSCR010000000
VERSION     DAOSCR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14051_length_4039_cov_3.311747"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PLT69_06135"
     CDS             <1..1207
                     /locus_tag="PLT69_06135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOJ14053.1"
                     /translation="DVERGGRLFCFDLSPLPLKPVWQIVACVRDVTVERFKERELVRI
                     KDQDKDMVEKAIDAVYIIRNGRFLLVNRKFQELFGYSADEILNRHFRHVMCRESIRVI
                     AGSLRSATGNVFVPNLEISARRKDGQKIYLEISIGKIKIDQDDCYVGVVRDITGKKEL
                     LAMKTRFLNVASHEIRVPLTVIRGYGRMLARDKDCALTPGQKECIDEIEKQCEKLLKF
                     SNSLLDFAKINDEAFSLNREWVDMADFIASVVRTMQVSADAKRVKIVFVNEGGVDRLS
                     VDPMRFEQALTNLIDNAIKHAPEGSEVTVSLGRNRQGPETLRMLLHQETAEISVRDRG
                     PGIKTEEARELFNDFFVGESGRAKGGIGLGLAITREIVHAHGGQVEARPSEEGGHFVI
                     TMPLKLKEA"
     gene            1246..2673
                     /locus_tag="PLT69_06140"
     CDS             1246..2673
                     /locus_tag="PLT69_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ14054.1"
                     /translation="MKEDIRILVVDDDESIRRYIVKLLTQGGYNVQAFSGGKEALREL
                     RSNQNYSLIILDILMPDMDGLETLSEIRKISKDIPVLMLSALGQTNIIVKAMKAGATD
                     YLVKPFEEEKLEVAITKSLEKRRLLNEISSLRRQLRIDELKGEFIYNSPKMQKVKELV
                     DQVAETDVSVLIEGESGTGKELVARALHYKSRLRDKPFVKVNCAALPHDLLESELFGY
                     ERGAFTGAYRSKPGKFELANNGTIFLDEIGEMDLAIQSKLLQVLQEGTFSRLGGKQDV
                     QVNVRVIAATNKDLKKAIEQGTFREDIYYRLNVVNIKIPPLTERREDITYLFEYFLDT
                     YNEKYGKAVQVSKEEIYPLLLEYPWPGNVRELKNQVKRLVILGNVDELVGYLKGSGKG
                     LVVERSFVDSRDRGLDSMGSFPEGDDEIEPLKVAAKEATIRAERNMILKALKGTNWNK
                     KKAASLLKVSYKALLYKIKECGIEK"
     gene            2700..3242
                     /locus_tag="PLT69_06145"
     CDS             2700..3242
                     /locus_tag="PLT69_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOJ14055.1"
                     /translation="MRHVVSLVLVFLGVSLVAWAQEPEYRIGPSDLLEISVWGEQTLS
                     RQVTVRADGFISMPLLGDVAVSGKTTAQVKEELESRLARFIKDPRCAVIVVEQRSKRF
                     YVQGQVARPGQYTLDQGLSLTQVISIAGGFTQWADKDDIVILRGETDAKTRITVDFDR
                     IVKGKDQDIPIQPGDTIIVP"
     gene            3239..>4039
                     /locus_tag="PLT69_06150"
     CDS             3239..>4039
                     /locus_tag="PLT69_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14056.1"
                     /translation="MTYRILPGIVVAATCLWPPSPLAALSFEVHPGLYQSYEYSDNYE
                     GEVADGQSEATYTVGPSITVTGESQRANLDLSGRYTRSYHRRNEEDDSPEAALSARAA
                     YRLERVGGSLAYSFMRTLTRDTLSSPFGERKVHSGDAEAAWDASQVVTLRAGCDITDE
                     RWSGRASDEEDVQSNGLDVGALVRIGPLNTLDVVARRAYVDYEVSRDVVETSGSARLE
                     RSFTPRLTGGLVVSYADERLRRPGDRAPLRVIHRGGLVLTVDTERLPDS"
BASE COUNT          884 a         1173 c         1331 g          651 t
ORIGIN      
        1 ggacgtggag cgcggcggca ggttgttctg cttcgatctc tccccgctgc cgttgaagcc
       61 cgtgtggcag atcgtcgcgt gtgtccgtga cgtgacggtc gagaggttca aggagcggga
      121 gctcgtccgc atcaaggacc aggacaagga catggtggag aaggccatag acgcggtcta
      181 catcatcagg aacgggaggt tcctcctcgt caacaggaag ttccaggaac tcttcggcta
      241 cagtgccgac gagatcctga accgccactt ccgccacgtc atgtgcaggg aatccatacg
      301 ggtgatcgcc ggttccctcc gctcggcaac cggcaacgtg ttcgtgccga acctcgagat
      361 cagtgcgaga cgaaaggacg ggcagaagat ctacctggag atcagcatag ggaagatcaa
      421 gatcgaccag gacgactgct acgtgggcgt ggtgcgcgac atcaccggga agaaggagct
      481 cctcgcgatg aagacgaggt tcttgaacgt ggcctcccac gagatcaggg ttcccctcac
      541 ggtgatccgg gggtacggaa ggatgctcgc cagggacaag gattgcgcgc tcacgcccgg
      601 gcagaaggag tgcatcgacg agatagagaa gcagtgcgag aagctgctga aattctcgaa
      661 ctccctgctc gacttcgcga agatcaacga cgaggcgttc tccttgaacc gagagtgggt
      721 ggacatggcg gacttcatcg cctccgtggt gcgtaccatg caggtgagcg ccgatgcgaa
      781 gcgggtgaag atcgtgttcg tgaacgaggg gggtgtcgac aggctctccg tcgaccccat
      841 gcgtttcgaa caggcgctca cgaacctcat agacaacgcg atcaaacacg cccccgaggg
      901 ctcggaggtc accgtgagcc tcggccgcaa caggcagggc ccggagaccc tgcggatgct
      961 cctccaccag gagaccgcgg agatatcggt gagggaccgg ggccctggca tcaagacgga
     1021 agaggcgcga gagctcttca acgatttctt cgtgggcgag agcgggaggg ccaagggcgg
     1081 catcggcctc gggctcgcaa tcacccgcga gatcgttcac gcgcacggcg gccaggtcga
     1141 ggcgaggccg tcggaggagg gaggccactt cgtcatcaca atgcccttga agttgaaaga
     1201 ggcataaggt attaagagaa cgggaaatta cagggtctgc cggtgatgaa agaagacata
     1261 agaatcctgg ttgtcgacga cgacgagagt atacggagat acatcgtcaa gctccttacc
     1321 cagggcggtt acaacgtgca ggcgttctcc ggaggcaagg aggcgttgag ggagctgagg
     1381 tcgaaccaga actactccct gatcatcctc gatatcctca tgcccgacat ggacggcctg
     1441 gagacgctct ccgagataag gaagatctcg aaggacatcc ctgtcctcat gctctctgcc
     1501 ctcggccaga cgaacatcat cgtcaaggcc atgaaggcgg gggcgaccga ctacctcgtg
     1561 aaacccttcg aggaagagaa actcgaggtc gcgatcacca agtccctcga gaagaggagg
     1621 ctcctgaacg agatatccag cctgaggaga cagctgcgca tcgacgagct caagggcgag
     1681 ttcatctaca acagtcccaa gatgcagaag gtgaaggaac tcgtcgacca ggtggcggag
     1741 acggacgtga gcgtcctcat cgagggggag agcggaacag gcaaggaact cgttgccagg
     1801 gccctccact acaagtcgcg cctcagggac aagcccttcg tcaaggtcaa ctgcgccgcc
     1861 ctgccccacg acctcctcga gtccgagctc ttcgggtacg agcggggcgc cttcacgggg
     1921 gcgtacaggt cgaagcccgg caagttcgag ctcgcgaaca acggcaccat cttcctcgat
     1981 gagatcggcg agatggacct cgccatccag tcgaagctcc tccaggtcct ccaggaaggg
     2041 accttctccc gcctgggcgg gaaacaggac gtgcaggtga acgtcagggt catcgcggcc
     2101 acgaacaagg acctcaagaa ggccatagag caaggcacgt tcagggagga catctactac
     2161 cggctcaacg tggtgaacat caagatcccc cccctgacag agcgccggga ggacatcacc
     2221 tacctgttcg aatacttcct cgacacctac aacgagaaat acggcaaggc cgtgcaggtg
     2281 agcaaggaag agatctaccc gctgctgctc gagtacccct ggccgggcaa cgtgagggag
     2341 ctcaagaacc aggtcaagag gctggtgatc ctcggcaacg tggacgagct cgtcgggtac
     2401 ctcaaggggt cgggcaaggg gctcgtcgtc gagaggagtt tcgtcgattc ccgggaccgc
     2461 ggcctggatt ccatgggttc gttcccggag ggcgacgacg agatcgagcc tctcaaggtg
     2521 gcagcaaagg aagccaccat cagggcggag aggaacatga tcctgaaggc cctcaagggt
     2581 acgaactgga acaagaagaa ggccgcaagc ctcctcaagg tgagctacaa ggcccttctc
     2641 tacaagatca aagagtgcgg gatcgaaaaa taatgaagac accgaaaggg ggggtcccca
     2701 tgaggcatgt ggtgtcgctt gtgctggtct tcctgggggt ctccctcgtg gcttgggctc
     2761 aggagcctga ataccgcatc ggcccttcgg atctcctcga gatctccgtc tggggcgagc
     2821 agaccctttc ccgacaggtg acggtcaggg cagacgggtt catctccatg ccccttctcg
     2881 gcgatgtggc cgtgagcggc aagaccaccg cacaggtcaa ggaggagctc gagtcgaggc
     2941 ttgcccggtt catcaaggac ccacgctgcg cggtcatcgt cgtggaacag aggagcaagc
     3001 gtttctacgt gcagggacag gtcgcccggc ccggccagta caccctcgac cagggcctct
     3061 cccttaccca ggtgatatcg atcgccggcg gtttcaccca gtgggctgac aaggacgaca
     3121 tcgtcatcct caggggcgag accgacgcca agacgaggat caccgtggac ttcgaccgta
     3181 tcgtcaaggg caaggaccag gacatcccca tacaacctgg agacaccatc atagtcccgt
     3241 gacgtatcgc atactccccg gtatcgttgt tgcggcgacc tgcctgtggc cgccctcgcc
     3301 gctcgcggcg ctttccttcg aggtccatcc agggctgtac cagtcgtacg agtactcgga
     3361 caattacgag ggggaggtcg cagacgggca aagcgaggcg acctacacgg tggggccgag
     3421 catcaccgtg accggggagt cgcagagggc gaacctggat ctctcaggca ggtacacacg
     3481 gtcgtatcac cgccggaacg aggaggacga cagcccggag gcggcgctct cggcgagggc
     3541 ggcctaccgg ctggagaggg tggggggatc cctcgcgtac tcgttcatga ggaccttgac
     3601 gcgcgacacc ctgagctccc cgttcggtga gcgcaaggtc cactcggggg atgcagaggc
     3661 cgcctgggat gcgagccagg tggtgacact gcgcgcgggg tgcgacatca cggacgagag
     3721 gtggtccggc agggcctccg acgaggaaga cgtgcagtcc aacggcctcg acgtgggcgc
     3781 cctggtgagg atcggcccgc tgaacaccct ggacgtggtc gcccggcggg cgtacgtcga
     3841 ttacgaggtg agccgtgacg tcgtggagac ctcgggttcg gcacgcctgg aacgatcctt
     3901 cacgcccagg ctgacgggcg gtctcgtggt ctcgtacgcg gacgagagac tgaggaggcc
     3961 gggcgaccgg gcgccgctga gggtgatcca tagaggcggc ctcgtcctga ccgtggatac
     4021 ggagaggctg cccgactca
//
LOCUS       DAOSCR010000201         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14169_length_4009_cov_3.925139, whole genome shotgun sequence.
ACCESSION   DAOSCR010000201 DAOSCR010000000
VERSION     DAOSCR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14169_length_4009_cov_3.925139"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLT69_06155"
     CDS             <1..301
                     /locus_tag="PLT69_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ14057.1"
                     /translation="TLGASPARTFVSVILPIVSRALAVGAVFAFTMSIGEFGATLFVA
                     RPANPTMTLAVYRYLSQPGDLNYGCAMAMSTLLMAVTTAGFLFLERVRFTGEGEL"
     gene            979..2079
                     /locus_tag="PLT69_06160"
     CDS             979..2079
                     /locus_tag="PLT69_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ14058.1"
                     /translation="MRILTLENVSKSFGLKRVIDGVDLSIDKGKTLCLLGPSGCGKTT
                     LLRIVAGLEKPDSGRILFEGSDITTLEPHRRRFGMMFQDYALFPHMDVLGNVAFGLRM
                     LDLPKDEVKGRAMEALKLVNMEGLARRNVVDLSGGERQRVALARTLAIRPRLVLLDEP
                     LGSLDRVMRERLVTEIRDILKGIGATCIFVTHDQAEAYAVSDMTAVMLDGRIVQVDPP
                     RLLCRNPRTPAVARFLGLANLVEGIPETDGTIRTPVGTFSSPPRPPFPRRPLTVLIHP
                     DAALWVRKASASDPGTPGPRVKGVVRSVVYRGRRLTVEILAAGGVTLSFEVPDGSWDM
                     EVGMEAVIAIDPLLVTVLEEQDEKGTQGPPRS"
     gene            complement(2254..>4009)
                     /locus_tag="PLT69_06165"
     CDS             complement(2254..>4009)
                     /locus_tag="PLT69_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOJ14059.1"
                     /translation="ELRIPYNPAWEEVRVEEAFVRSPKGAVHTIDPREINVMDQPWAG
                     TAPRYPAGKILVVSFPGLEEGSTISYRYAFTSKGWNEPAFVEPFQAMEPVGDKTVRVE
                     CPPGVNLRVITADNGFGATRAWRPFPRGFVSLSQGEDRGMEVKILQAKNVPPAMREEM
                     LPALYAFAPSMAVASMPTEMFAASMSEILARQASERSFASAKAREIVRGLSGDRERIE
                     AVWRYVARHVRLVDIPLAESLPHMMSGADTTLADGYGHQADRAVLAAAMLTSLGFKTD
                     FVLATSIPEGDPLFDLARGFPATSLFVSLLVRVSTPSGDISIGDTDQYAPLGATVHAG
                     MYGLTLSSMAWETITVPSAEIGNKTETRVLMRVNASGDASVTFRKTYFGSSATSFIRA
                     ALEMTPEEKRRAFEEIVTSVSRSALATSPRVISVDRYPPSEEFSLEIKGYAGKSGDVM
                     TVRIPWLMRALPLVSSEERVHPLFTGPPREAVVNIEIELPEETQEVVSMPMGAYAAAP
                     AGTASVSMEGMLEGEGSGRKVVISQRVSTKPGTVWPDRYPELVSTHKALSAADASLVV
                     VRLGAGENRGGAQARDKE"
BASE COUNT          637 a         1308 c         1356 g          708 t
ORIGIN      
        1 caccctgggc gcctctcccg cacgaacgtt cgtctcggtg atcctgccga tcgtgagcag
       61 ggcgctcgcc gtgggcgccg tgttcgcctt caccatgtca atcggcgagt tcggcgccac
      121 gctcttcgtg gcaaggcccg ccaaccccac catgacgctc gccgtgtacc ggtacctctc
      181 acagccggga gatctcaact acgggtgcgc catggccatg agcactctgc tcatggccgt
      241 gacaacggcc ggcttcctct tcctcgagcg ggtgcgcttc accggtgagg gggaactgtg
      301 aggtgtctta ggcggcggcc attcgaaggc gcgaaccgtg ccccgggcat gaccgtttcg
      361 acggccggct agggatcatg gggaccgggc gcggttcccc ctctcggggc tcacgtggtt
      421 ccgggatagg gagcgttgcg cgggcggcgt ccgggtgtgg tcgagctgga agcccccctg
      481 cccatggggt tgccgtgcgg cccgtcatgt catagaagga ggcggaaggc cccatgatcg
      541 ggccggaggg tgacgggctg ctggatgtgc atgcaccagg tgcgcgttcc attccgcgtg
      601 acggccggcc gccacgccgg ggtgggacac ggccgggtgt acccgtcacg aggggcggca
      661 tgaatctgtc catgggacgg ggactggttt tggcatgagg cccttcacag gtggccgcgg
      721 agcgaggtgg acggaatcgg caacgctgag ggaactcctc gtgggtgtcg gccgtcccca
      781 ggggggagag acagttgatg gcaagcctgt cacgatggcc cggcgtaccc ggccatccac
      841 aggggggaga gactttgata aaagggtctg gcgtggggat gcgatgaaga tccaggacca
      901 tcggcgcttg agggctgcaa cgggagtgcc ccacaggcgg tatgacaggg acgatggcat
      961 gcggcgggga accatgagat gcgtatcctg acgcttgaga acgtctccaa gagcttcggc
     1021 ctgaagaggg tgatcgacgg ggtggacctc tccatagaca aggggaagac cctgtgcctc
     1081 ctgggcccct cgggatgcgg caagaccacg ctcctcagga tcgtggcggg cctcgagaaa
     1141 cccgacagcg ggcggatcct gttcgaaggg tcggacatca ccacgctcga gccccatcgc
     1201 cggcgtttcg gcatgatgtt ccaggactac gccctgttcc cccacatgga cgtgctcggc
     1261 aacgtcgcat tcgggctgag gatgctcgac ctcccaaagg acgaggtcaa aggtcgcgcc
     1321 atggaggctc tcaagctggt caacatggag ggcctcgcca ggcgcaatgt cgttgatctc
     1381 tcgggcgggg agcgccagag ggtcgccctt gcgcgcacgc tcgccatcag gccgaggctc
     1441 gtgctcctgg acgagcccct tgggtcccta gaccgggtca tgagggagag gcttgtaacc
     1501 gagatcaggg atatcctgaa ggggatcggg gcgacctgca tcttcgtcac ccacgaccag
     1561 gcggaggcct atgcggtctc ggacatgacg gcggtcatgc tcgacggccg catcgtgcag
     1621 gtcgatcccc ccaggctcct gtgccgcaac cccaggacac cggcggtcgc caggttcctg
     1681 ggactggcga acctcgtgga gggaatcccc gagacggacg gtaccatacg gacacccgtg
     1741 ggtacgttct cttcgcctcc acggccgcct ttcccccgca ggcctctcac cgtgctcatc
     1801 cacccggatg cagcactgtg ggtgcgcaag gcctccgcgt cagacccggg caccccgggt
     1861 ccacgggtca agggtgtggt gcgctcggtc gtgtaccggg gcaggcgcct caccgtggag
     1921 atactggccg cgggcggcgt gacgctctcc ttcgaggtgc ccgacgggtc ctgggacatg
     1981 gaggtgggca tggaagcggt catcgccatc gaccccctcc tggtgacggt gctcgaggag
     2041 caggacgaga agggcaccca aggcccgccg cgttcttgat gcatctggat cggggccttc
     2101 ccgacgcgcc cgaaaccctg ggtgtcacca cgcaccaggg ggtgcatgca tcgcaggcag
     2161 gggacgtctt tcggtacgtc cccgtgaacg tgtctcgtgg gcacggccgg ccgtgatctc
     2221 ggccgacccc cggtgacggc atgaccgggt ctgtcactcc ttgtcccgcg cctgggcgcc
     2281 gccccggttc tcccctgccc cgagccgcac cacgaccagg gatgcgtcag cggccgagag
     2341 cgccttgtgg gtgctcacga gctcgggata cctgtccggc cagacggtgc ccggtttggt
     2401 ggataccctc tggctgatga cgaccttcct tccggatccc tcgccttcga gcatcccctc
     2461 catgctcacc gaggccgtcc cagcaggtgc agccgcgtag gcccccatcg gcatggagac
     2521 cacctcctgc gtctcctcgg ggagttcgat ctctatgttc acgaccgctt cacggggggg
     2581 gcccgtgaag agagggtgca ccctctcctc cgagctcaca agggggagcg ccctcatgag
     2641 ccacgggatc ctcacggtca tgacgtcgcc gctcttgccc gcatatccct tgatctcgag
     2701 tgagaactcc tcggagggag gataccgatc gaccgagatc acccgggggg aggtcgcgag
     2761 ggccgacctg ctcaccgagg tgacgatctc ttcgaaggcc ctcctcttct cctctggtgt
     2821 catctcgagc gcagccctga tgaacgaggt cgcgctcgac ccgaagtagg ttttcctgaa
     2881 cgtgacggac gcgtcacccg aggcgttcac cctcatgagg accctggtct cggtcttgtt
     2941 gccgatctct gcagagggga ccgtgatcgt ctcccacgcc atggacgaga gggtgagccc
     3001 gtacatgccg gcatgtacgg tggcgccaag gggtgcgtac tggtccgtgt ccccgatgga
     3061 gatgtctcca gacggggtgc tcaccctcac gaggagggag acgaacaggg aggtcgccgg
     3121 gaaccccctc gccaggtcga agagggggtc tccttcgggt atcgaggtgg cgagcacgaa
     3181 gtccgtcttg aagccgagcg aggtgagcat ggccgctgcg aggaccgcgc ggtccgcctg
     3241 gtggccgtac ccgtccgcga gcgtggtatc cgcaccgctc atcatgtgcg gcaggctctc
     3301 cgcgaggggg atgtccacga gacggacgtg cctcgccacg tacctccaca ccgcctcgat
     3361 acgctcccgg tcacccgaaa ggccccttac gatctccctg gccttggccg aggcgaagct
     3421 cctctctgag gcctggcgtg cgaggatctc gctcatgctc gcggcgaaca tctcggtcgg
     3481 catcgaggcg acggccatgg acggcgcgaa ggcgtacagg gcggggagca tctcctccct
     3541 catcgcggga gggacgttct tcgcttgcag gatctttacc tccatgcccc tgtcttcgcc
     3601 ttgggagagg gacacgaacc ccctcgggaa cggccgccag gcgcgtgtcg ctccgaagcc
     3661 gttgtccgcc gtgatcacgc gcaggttcac gccgggtggg cactcgaccc ggacggtctt
     3721 gtccccgacg ggctccatcg cctggaaggg ttcgacgaac gcaggttcgt tccagccctt
     3781 gctcgtgaat gcgtacctgt aggatatggt gctgccctcc tcgagccccg ggaagctcac
     3841 cacgagtatc ttccctgcgg ggtacctggg tgcggtcccc gcccagggct ggtccatcac
     3901 gttgatctca cggggatcga tcgtgtggac ggcgcctttg ggcgacctga cgaaggcctc
     3961 ctctacccgc acctcttccc aggctgggtt gtacggtatc ctcagctcg
//
LOCUS       DAOSCR010000202         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14184_length_4006_cov_3.389522, whole genome shotgun sequence.
ACCESSION   DAOSCR010000202 DAOSCR010000000
VERSION     DAOSCR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14184_length_4006_cov_3.389522"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLT69_06170"
     CDS             complement(<1..423)
                     /locus_tag="PLT69_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOJ14060.1"
                     /translation="MDTTYARGVLDVSQGEALARTRKNPYHCVMDLQRVLGASELFGG
                     MGGRSLERLLAMGRQKDYARGDILFMEGTPGNEFFLLLEGEVRLYKTTPGGQDVSLRI
                     VRPAEVFAEVILFEEHAYPVNAQALGPVRVFAVPRDGFL"
     gene            453..1310
                     /locus_tag="PLT69_06175"
     CDS             453..1310
                     /locus_tag="PLT69_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ14061.1"
                     /translation="MKINRPIVEIDREKCDGCGRCVISCAEGALEIVDGKARLTGEFL
                     CDGLGACIGECPRGALRIVVRESEAFDREAARTPGGPVHGDAARRPQMSPTDGIQPVS
                     ERRREHLDEARPGVLACGCPSSTAVTLQPGALAREHAGAHRSHLAHWPVKLALLNPSA
                     PFLRGSHMVLIADCSAAALYDLHTRILSGRTVAMACPKLDDADRHAVRLSEILRTSPP
                     SSLTVVRMEVPCCGGLERIAVRAAELSGTSVPMRRMVIGRTGEVIEEGDIPLTRRVKD
                     SGRGESVTG"
     gene            1455..1531
                     /locus_tag="PLT69_06180"
     tRNA            1455..1531
                     /locus_tag="PLT69_06180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Asp,seq:gtc)
     gene            1765..3312
                     /locus_tag="PLT69_06185"
     CDS             1765..3312
                     /locus_tag="PLT69_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ14062.1"
                     /translation="MTFLFLNPPVVKPCEPPPGLARIVGTLRGEGIRCKVIDANAEGL
                     LWLMRRAPSPCGARSARALAGLGKNLGLMRNGGAFGSIGRYIKTVLDIDHVLELAGRR
                     AGYTVGLADLRHDALSPLRSSDLVHAADHPEENVFYPFLEERILHALDEVKPDVFGVS
                     LSFLSQALCAFASIGIVRRHAPGLRIVLGGSLVTSWARGPGTASAFSGLVDEVVEGPG
                     EARLLELAGLGKGSVPLRYDFSPFNDEANLSPRPVIPVSCSTGCYWNRCSFCPERAEG
                     HRYRPNAPQEVLGQVDVLARGRETPLVHFTDNAMSPSLLEHVARSGRTFSWYGFARFT
                     PHLADADFCRALSRSGCVMLQLGLESGDQDVLDSLDKGISLQTAARSLVALKESGIGT
                     YVYLLFGTPQEDEDSARKTLAFIEAHAHLIDFLNLSIFNLPRGSREALELETYDFFEG
                     DLSLYTGFVHPKGWDRRRVRRFLDREFRRNEAVGKILRNSPPVFTSNHAPFFLMERRR
                     QDLRKGA"
     gene            3841..>4006
                     /locus_tag="PLT69_06190"
     CDS             3841..>4006
                     /locus_tag="PLT69_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ14063.1"
                     /translation="MAVQEKTLNMALFCDFENIALGVRDAKYAQFDMKKVLDRLLLKG
                     SIVVKKAYCDW"
BASE COUNT          690 a         1308 c         1328 g          680 t
ORIGIN      
        1 caggaagccg tcgcgcggca cggcaaagac ccggaccggt cccagggcct gggcgttcac
       61 ggggtaggcg tgctcctcga agaggatcac ctccgcgaac acctctgcgg gcctgacgat
      121 cctcaaggag acatcctgcc cgccgggggt ggtcttgtac aggcgcacct cgccctcgag
      181 gaggagaaag aactcgttgc cgggggtgcc ctccatgaac aggatgtcgc ctcgcgcgta
      241 gtccttttgc ctgcccatgg cgaggagcct ctcgaggctc ctgccgccca tgcccccgaa
      301 gagctccgat gcgcccaaga cccgctggag atccatgacg caatggtagg ggttctttct
      361 ggtccttgca agcgcttccc cttgactcac gtcaaggacg ccgcgcgcgt acgtggtatc
      421 catgatgcaa atgaacgaac cgggagggat gcatgaagat caacagaccc atcgtggaga
      481 tagaccggga gaaatgcgac gggtgcggca ggtgcgtgat ctcctgcgcc gagggcgcac
      541 tcgagatcgt cgacggcaag gcgaggctca cgggtgagtt tctgtgcgac ggtctcggcg
      601 cctgtatcgg tgaatgcccg agaggcgccc tcaggatcgt ggtgagggag tcggaggcgt
      661 tcgacaggga ggctgccagg acaccgggag gcccggtgca cggcgatgct gctcgacgcc
      721 cgcagatgtc gccgaccgat gggatccagc cggtcagcga gcggcgtcgg gaacacctcg
      781 acgaagcccg tccgggcgtc cttgcgtgcg gctgcccttc atccacggcc gtgacccttc
      841 agccgggcgc gcttgcccgt gaacatgcgg gtgcgcatcg ttcccacctg gcgcactggc
      901 cggtgaagct ggccctcttg aacccgtcgg caccctttct caggggttcg cacatggtcc
      961 tcatcgccga ctgttcggcc gctgccctgt acgacctcca cacccggatc ctgagcggca
     1021 ggaccgtggc catggcctgc ccgaagctgg acgacgccga ccggcatgcg gtgaggctct
     1081 cggagatcct caggacctca ccgccctcga gcctcaccgt ggtgcgcatg gaggtgccct
     1141 gctgcggggg gctcgagcgc atcgccgtaa gggctgcaga gctctcgggc acgtccgtcc
     1201 ccatgcgccg catggtgata ggacgcacgg gggaggtgat cgaggaaggc gacatcccct
     1261 tgacaaggcg agtgaaggac tcaggtcgcg gggagtcggt gacggggtga tcgaggaggg
     1321 cgacatcccc cttgccaagg gcacgaaggt gcgtgtatag ctatgtcgcc cttgtgttcg
     1381 ccgaggcgtg gcatattata tagagaggaa aggcctcgcc cttgaggcgc tcgggcggaa
     1441 gaaccagacg gtgcgggggc gtagttaagt cggtaataac gccggcctgt cacgccggag
     1501 gtcgcgggtt cgagtcccgt cgctcccgcc acttttccat gaccccgcca cggttgcgtc
     1561 atgcctgaga gagaaccctg gagatgcccg gcggcaccca ggtccctctg aactcccggg
     1621 gcacgctggg gtcaatgcac gcaccatcga tgcgtccgtc ccgcgggccg gggctcgcag
     1681 ccgcacaggg ccagagaagc cctgcggccg ggggcatctt ccttcttctt gactttcccc
     1741 caggctcctg attcaagctc ccccatgacc ttcctgttcc tgaacccgcc ggtggtgaaa
     1801 ccatgcgagc cccctcccgg gctcgccagg atcgtcggga cactcagggg cgaagggatc
     1861 cggtgcaagg tgatagacgc caacgcggag ggtctcctct ggctcatgcg cagggcgcct
     1921 tccccgtgcg gcgcccgttc ggcccgcgca ctcgcaggcc tcgggaagaa cctgggcctg
     1981 atgagaaacg ggggcgcctt cgggagcatc ggcaggtaca taaagacggt cttggacatc
     2041 gatcacgtcc tcgaactggc cggtcggcgg gccgggtata cggtcggcct cgccgatctg
     2101 aggcacgacg ccctgtcccc cttgaggagc agcgacctcg tccatgccgc agaccatccc
     2161 gaggagaacg tcttttatcc cttcctcgag gagcggatcc tgcatgcgct cgacgaggtg
     2221 aagccggatg tcttcggcgt gtccctgtcg ttcttgagcc aggccctgtg cgccttcgca
     2281 tccatcggca tcgtgagacg ccacgcgccc ggcttaagga tcgtgctcgg cgggtccctg
     2341 gtcacctcgt gggcacgagg gccaggtacg gccagcgcct tctcggggct ggtcgacgag
     2401 gtggtcgagg ggccgggcga ggcgcgactc ctcgagctcg cgggtctcgg gaaggggtca
     2461 gtgccgctga ggtacgactt cagcccgttc aacgacgaag cgaacctctc gccccgcccg
     2521 gtcatccccg tgagctgttc gacgggatgc tactggaaca ggtgctcgtt ctgcccggaa
     2581 cgggccgaag ggcatcggta caggccgaac gcgcctcaag aggtactggg acaggtcgac
     2641 gtcctggctc gcggcaggga aacgccgctt gtccacttca ccgacaacgc catgagccct
     2701 tccctgctcg agcacgtcgc ccgctcaggg cggacgttct cctggtacgg tttcgcgcgg
     2761 ttcaccccgc acctcgcgga tgccgacttc tgcagggccc tgtcgcgctc gggctgcgtc
     2821 atgctccagc tcggcctgga gtcaggcgac caggacgtgc tcgattccct ggacaagggg
     2881 atctccctcc agaccgccgc acgctcgctg gtggccctga aggagtcggg catcggcacc
     2941 tacgtgtacc tcctgttcgg cacgccccag gaagacgagg actccgcgcg gaaaaccctc
     3001 gccttcatcg aggcgcacgc acacctcata gacttcctca acctctccat attcaacctc
     3061 ccgcgcggct cgagggaggc cctcgagctc gagacgtacg acttcttcga gggcgatctt
     3121 tccctgtaca ctgggttcgt ccaccccaag gggtgggata ggaggcgcgt gaggcggttc
     3181 ctcgacaggg agttcaggag gaacgaggcc gtgggcaaga tcctgagaaa ctcgcctccc
     3241 gtgttcacct cgaaccacgc gccattcttc ctcatggaga gacgcaggca ggatctccgg
     3301 aaaggggcgt gacccgccgt ccgtcatggc gcggccatgc ccgacccttc ggcatcgggc
     3361 cgtcggggga ggattcggcg gaccccgcgg gcctcgggcg acgcacggtg tgcaggcgcc
     3421 cgttcgcaca ccagcgcagc cctgactgtc ctgaacgttc ccccctcagg cgactccccg
     3481 tttgcagatc gaaagaagtc catggcctgt acaggcaggc catacctgaa cgttccccac
     3541 ctctggcggc gcccggtttg cgggcacccc ttcccaccgc cctcggggcc tgtccatccc
     3601 aggtcgcctg cgcgccttgg caacagatct ccttgacagg acgggtgaaa gaacggatga
     3661 atggtgaagg ccgccaagtc ttgccacacg aggaaagctg agaagtcggc gcatggcttc
     3721 ggcacctgtg tggatacggg cgggacccgt caagcccgtg tgggagcgca ccgggacgtg
     3781 caccccgggc ggcggccggg agcgttgccg gtaccctgaa aaaaacgcga gggatggttc
     3841 atggccgtac aggaaaagac actgaacatg gcgctgtttt gcgatttcga gaacatagcc
     3901 ctgggcgtga gggacgccaa gtacgcccag ttcgacatga agaaggtgct cgatcgactg
     3961 ctgctcaagg gcagcatcgt cgtcaagaag gcgtactgcg actggg
//
LOCUS       DAOSCR010000203         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14230_length_3996_cov_3.292819, whole genome shotgun sequence.
ACCESSION   DAOSCR010000203 DAOSCR010000000
VERSION     DAOSCR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_3996_cov_3.292819"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLT69_06195"
     CDS             complement(<1..253)
                     /locus_tag="PLT69_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14064.1"
                     /translation="MRTSADKPPVHEVSGAALAFFLWFVTFYLGGPNFWLKITLSSLA
                     LAVYASWRGALEQNVLSFDLKAIGQGVVADARPGAEDRHR"
     gene            complement(250..1140)
                     /gene="menA"
                     /locus_tag="PLT69_06200"
     CDS             complement(250..1140)
                     /gene="menA"
                     /locus_tag="PLT69_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOJ14065.1"
                     /translation="MSLYTDWFLASRPWSFTMTAISVGVGAAVGAIDGPFSWPLFLAT
                     LAGAVCMHASANLFNDYFDVKYGVDTAEAATAKYRPHPLVQGAISPRQVFVAAASLLG
                     LGAAVGVALALHAGWTVIWIGLAGALAAVGYTAPPLKYKYLALGEVSVFLMWGPLMVE
                     GAYVVQRGHASLQALLVSVPFGILVALVLLANNIRDINHDRGRGVKTLANVLGGRSSL
                     VLYAGLMVAAFCCIPLFVLTETLPVWGFVTFLSLPVAVGLLRLMATEVPEDADARTAK
                     LDTAFGVLLLASLVIQRFFG"
     gene            complement(1481..>3996)
                     /gene="ileS"
                     /locus_tag="PLT69_06205"
     CDS             complement(1481..>3996)
                     /gene="ileS"
                     /locus_tag="PLT69_06205"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOJ14066.1"
                     /translation="GWDCHGLPIEHQVEISLGEKIAGMSKAEVRRHCREYAERFLSIQ
                     RDEFKRLGVFGLWEEPYVTMSYDYEATIVRELGRFIDQGSIYRARKPVYWCATCQTAL
                     AEAEVEYHDQATPAIFVRFRLTSDVSGRVPELAPFKDDTHVVIWTTTPWTIPANLAVA
                     FHPDFTYAAVRTKPYGVLIMAEDLVPSCMSTFGVTDYEVLAVFKGTAMEHLKARHPLY
                     DRESLFILAPFVTLDAGSGCVHIAPGHGEEDYEIGKAYGLEIYAPVDNEGRFTSEVEG
                     FEGRYVFEANDDVNRALKDRGALLHLSDYDHSYPYCWRCKNAIIFRSTNQWFISLEHA
                     DLRKKALEAITKVRWIPSWGKDRIYSMIENRPDWCISRQRAWGVPIPVFYCRNCGEIL
                     ASGDVARHVAELFEKEGADAWFVHPEKDLLPEGTRCAKCSGDDLAKEEDIVDVWFDSG
                     VSYAAVCEKDPRLGVPVDMYLEGSDQHRGWFHSTLLASVGTRGEAPYRTVLTHGFVVD
                     GEGRKMSKSLGNTIAPQEIIDRYGAEILRLWVSAQDFRNDIRISQEIVERLVETYRRI
                     RNTARFMLGNLWGFDPSRDMVPYGDMLEMDRYALHVTQELISRVRRAYEEFEPYVIYQ
                     LVHNFCVVDMSAFYLDVLKDRLYVFKKDSLARRSAQTALFRIVKDLTRLIAPILAFTA
                     EEIWDHIPGFEHKAESVHLSDMPEPDPHLMDDGLKQRWERILALRQEVSKVLEGLRKD
                     KTIGHSLDACVKLRASGETLSFLQDVQGLLADVFICSKVEVEEGEGPWIQSEVLGAVA
                     VEAHRAPGVKCPRCWRYAEDIGTSASHPEICGRCASQIG"
BASE COUNT          679 a         1341 c         1301 g          675 t
ORIGIN      
        1 cgcgatggcg gtcctccgcg ccggggcgcg catccgcgac gacgccctgg ccgatcgcct
       61 tcaggtcgaa ggagaggacg ttctgctcga gcgcacccct ccaggatgca tagaccgcga
      121 gagccaggga cgagagcgtt atcttcagcc agaagttcgg cccgcccagg tagaaggtca
      181 cgaaccagag gaagaacgcc agggccgcgc ccgacacttc gtgtaccggg ggtttgtcgg
      241 cacttgtcct catccgaaga acctctggat gacgagcgag gcgagcagca gcacaccgaa
      301 ggcggtgtcc agcttcgcgg tccgggcgtc cgcgtcctcg ggcacctccg tcgccatcag
      361 gcgcaggagg ccgacagcga ccggcaggct caagaatgtg acgaaacccc acacgggaag
      421 ggtttccgtg aggacgaaca gggggatgca gcaaaaggcc gcgaccatga gccctgcgta
      481 cagcaccagg ctcgaccggc cgcccaggac gttggcaagc gtcttcaccc cccggcccct
      541 gtcgtggttg atgtccctga tattgttcgc gagcaagacg agcgccacga ggatcccgaa
      601 gggaacggac acgaggagcg cctgcaggct cgcatggccc cgctgcacga cgtaggcccc
      661 ctcgaccatg agaggacccc acatgaggaa caccgacacc tcgcccaggg cgaggtactt
      721 gtacttcaag ggcggggccg tgtagccgac ggccgcgagc gcccctgcga gacctatcca
      781 gatcaccgtc cagcccgcgt ggagggcgag cgcgacgcct acggccgccc cgaggccgag
      841 gagggaggct gccgcaacga agacctggcg ggggctgatc gcgccctgga cgagggggtg
      901 ggggcggtac ttggccgttg ccgcctcggc ggtgtccacc ccgtacttta cgtcgaagta
      961 gtcgttgaac aggtttgcag aggcgtgcat gcagaccgcg cctgcgagcg tggccagaaa
     1021 gagcggccac gagaagggcc cgtcgatcgc ccccacggcc gccccgacgc ccaccgagat
     1081 ggcggtcatg gtgaaagacc acggtctgct ggccaggaac cagtctgtat acaggctcat
     1141 gagagtgcac ctcccgaacg cgccggcctg tgtgcaccgg ctggtcttgt gtcaggtccc
     1201 gaacctccca cgggcagcgg gacacgtctc ttgtcagggc gctgagaaac cggcccgccg
     1261 aggggccggg acgggtcgaa cccctgcgtt cgggatgcga acctcccctc cacgtgcacc
     1321 cgggatccct gaggtaccga ggagcgcgcc gaggcgggag aggacacgac gagggccgga
     1381 agatcccgtc tccgccatct cgtttcaggc cggggacctg gggccccgtc cccccggggg
     1441 cgtttgcggg tgcccatcga gcccggcgtc tctttcctgg tcacccgatc tgcgaggcgc
     1501 atctcccgca gatctcgggg tgcgaggcgc ttgtcccaat gtcctctgca tagcgccagc
     1561 accgcgggca cttgaccccg ggcgccctgt gcgcctcgac ggcgacagcg cccaagacct
     1621 cgctctggat ccaggggccc tccccttctt ccacctcgac cttggagcag atgaacacat
     1681 ccgcgagcag cccttggacg tcctgaagga aggacagggt ctcgcccgag gccctcagct
     1741 tcacgcaggc gtccagcgag tggccgatgg tcttgtcctt gcgcaggccc tcgaggacct
     1801 tcgatacctc ctgcctgagc gccaggatgc gttcccagcg ctgcttcaac ccgtcgtcca
     1861 tcaggtgagg gtcgggctcc ggcatgtcgg agaggtggac gctttccgcc ttgtgctcga
     1921 agccggggat gtggtcccag atctcctcgg cggtgaacgc gaggatgggc gcgatcaacc
     1981 tcgtgaggtc cttcacgatc ctgaagaggg ccgtctgggc cgacctcctg gcgagcgagt
     2041 ccttcttgaa cacgtacagg cggtccttga ggacgtccag gtagaaggcg ctcatgtcca
     2101 ccacgcagaa gttgtgcacg agctggtaga tgacgtaggg ctcgaactcc tcgtacgccc
     2161 ttctcacccg cgatatgagc tcctgcgtga cgtggagcgc gtaacggtcc atctcgagca
     2221 tgtcgccgta gggcaccatg tcgcgggacg ggtcgaaccc ccagaggttg ccgagcatga
     2281 agcgcgccgt gttgcggatc ctgcgatacg tctccaccag gcgctcgacg atctcctgcg
     2341 agatgcggat gtcgttcctg aagtcctggg cgctcaccca gagcctcagg atctcggccc
     2401 cgtacctgtc gatgatctcc tggggggcta tggtgttgcc gagcgacttc gacatcttcc
     2461 tgccctcgcc gtccacgacg aacccgtggg tgaggaccgt cctgtacggc gcctcccccc
     2521 gcgtccccac ggaggcgagc agggtgctgt ggaaccaccc gcggtgctgg tccgaaccct
     2581 cgaggtacat gtcaaccggc accccgagcc tcgggtcttt ctcgcacacc gcggcgtagc
     2641 tcacgcccga gtcgaaccac acgtccacga tatcctcttc cttggcgagg tcatcccccg
     2701 agcacttcgc gcacctcgtc ccctcgggca ggagatcctt ctcggggtgc acgaaccagg
     2761 cgtccgcacc ctccttctcg aagagttcgg ccacgtgcct cgccacatcg ccgctcgcga
     2821 ggatctcccc gcaattgcgg cagtagaaca cggggatcgg gaccccccat gccctctggc
     2881 gcgagatgca ccagtcaggc ctgttctcga tcatggagta gatgcggtcc tttccccagg
     2941 agggtatcca cctgaccttc gtgatcgctt ccagcgcctt cttcctgagg tcggcgtgct
     3001 ccagggagat gaaccactgg tttgtcgagc ggaagatgat ggcgttcttg caccgccagc
     3061 agtacgggta cgagtgatcg tagtccgaca ggtgcaggag ggcgcccctg tctttcaggg
     3121 cccggttcac gtcgtcgttg gcctcgaaca cgtatctgcc ctcgaacccc tccacctcgg
     3181 aggtgaacct gccctcgttg tcgaccgggg cgtaaatctc gaggccgtac gccttgccga
     3241 tctcgtagtc ttcctcgccg tgaccgggcg cgatgtgcac gcagcccgaa ccggcgtcca
     3301 gcgtcacgaa tggggccagg atgaagagag actccctgtc gtagagcggg tgacgcgcct
     3361 tgaggtgttc catggccgtg cccttgaaga cggccaggac ctcgtagtcc gtgacgccga
     3421 aggtcgacat gcacgagggg acgaggtcct cggccatgat gagcacgccg tagggtttcg
     3481 tcctgaccgc ggcgtaggtg aagtccgggt ggaaggccac ggccaggttc gcggggatgg
     3541 tccagggggt ggtggtccat atcaccacgt gggtgtcgtc cttgaagggc gcgagctcgg
     3601 gcacgcggcc ggacacgtcc gaggtcaacc tgaagcgcac gaagatggcg ggtgtggcct
     3661 ggtcgtggta ctccacctcc gcctcggcga gcgcggtctg gcaggtcgca caccagtaca
     3721 cgggcttgcg ggccctgtag atcgagccct ggtcgatgaa cctgcccagt tcccggacga
     3781 tggtcgcctc gtagtcgtac gacatggtca cgtacggctc ttcccagagc ccgaagacgc
     3841 ccaggcgctt gaactcgtcc ctctggatgg acaggaaccg ctccgcgtac tcgcggcagt
     3901 gcctgcgcac ctccgccttg ctcatgcccg cgatcttctc gcccagggag atctccacct
     3961 ggtgctcgat gggcagcccg tggcagtccc acccgg
//
LOCUS       DAOSCR010000204         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14251_length_3991_cov_4.282520, whole genome shotgun sequence.
ACCESSION   DAOSCR010000204 DAOSCR010000000
VERSION     DAOSCR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14251_length_3991_cov_4.282520"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLT69_06210"
     CDS             <1..870
                     /locus_tag="PLT69_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOJ14067.1"
                     /translation="VFEDLDLKKKIFADLDAKAPEHAILASNTSSMSITEIASAAKRP
                     GRVVGMHFFNPAVMLKLVEVIYARQSTDEAIQTTFDVAKKMGKVPVIVRKDSPGFIYN
                     RVNAPTALLLQLILDKGSPTPAQFDAAFKGLMPMTPFELLDYVGLDIVLHTQDYYSKT
                     LSKDYTPRKALKDLVASGNLGKKTGRGIYDWSAGRPAIGADNPTTEYDFTHMVALQVN
                     EATKCLEEGVTNDPKDIDLAIANGGGGMGPFTLAQSIGYDKLVARCNELADKFGIETF
                     RPTKTMQEGKIKV"
     gene            1022..1627
                     /locus_tag="PLT69_06215"
     CDS             1022..1627
                     /locus_tag="PLT69_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14068.1"
                     /translation="MKGSILVLGTLITLILFTTAVFAGNPSKATQADAKKDTLIVESP
                     DAHGQDMESGKGANPKPDGADWEKKDKGKTVTGTGSQDSKKGKDAQERPKTQQGDDKK
                     TDKVKDAKDKDVAGKPGHDMARKETVVGYVMTIDNLTGLIVVKEENKKTERAIVVNAR
                     DLASLSAGAKVNIELAPGTNTAEKISVIAEGKGGAKSKRKK"
     gene            complement(1779..2507)
                     /gene="prxU"
                     /locus_tag="PLT69_06220"
     CDS             complement(1779..2507)
                     /gene="prxU"
                     /locus_tag="PLT69_06220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_232362698.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2256..2258),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOJ14069.1"
                     /translation="MPEEIKAGCARPTGGPVGEKPVEAPPVLAGERKEAQVMIKVGAK
                     APDFTAPAYFKGTFVQVKLSDYLGKWVVLCFYPGDFTFVUATEVSAVAEKYPQFEKLG
                     VEVLSMSVDSVFVHKMWNDHELSRMVDGGVPFPMLSDGGGRVGSVYGVYDPDAGVEMR
                     GRFIIDPDGVIQGYEVLTPPVGRNVGETIRQIQAFQHVRASKGTEATPSGWRPGKLTL
                     KPGPGLVGKVWEVWKPSMESEENP"
     gene            complement(3217..>3991)
                     /gene="zupT"
                     /locus_tag="PLT69_06225"
     CDS             complement(3217..>3991)
                     /gene="zupT"
                     /locus_tag="PLT69_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOJ14070.1"
                     /translation="GFSAGVMLYVSFVEIFFKGLESLEGRYGDWGHWINAASFFSGML
                     LIGLIDTLVPAQDNPHETHSEAETMVLHDPSAQMPRPRGSSSQAQGLQENTPHGRKLM
                     RMGLFTALAIGIHNFPEGLATFLAALQDPGLGAAIAVAVALHNIPEGISVSVPIFYAT
                     GNRRKAFFYSLLSGLAEPVGAGIAYLALMVFVGGASGMIPAEVMGVLFGGVAGIMVYI
                     SLDELLPTSRAYGKGHDSLLGLVAGMVVMALSLLLMRTS"
BASE COUNT          789 a         1349 c         1205 g          648 t
ORIGIN      
        1 gtgttcgagg acctggacct caagaagaag atcttcgccg acctcgacgc aaaggcgccg
       61 gagcacgcca tcctcgcgag caacacctcg tccatgagca tcaccgagat cgcatccgcc
      121 gccaagagac cgggccgggt ggtgggcatg cacttcttca accccgcggt catgctcaag
      181 ctcgtggagg tcatctacgc caggcagtcc acggacgagg cgatacagac cacgtttgac
      241 gtcgcgaaga agatgggcaa ggtgcccgtg atcgtgagga aggactcgcc gggtttcatc
      301 tacaaccggg tgaacgcccc cacggcgctc ctgttgcagc tcatcctgga caagggcagc
      361 ccgacgcccg cccagttcga cgcggcgttc aagggcctca tgcccatgac ccccttcgag
      421 ctcttggact acgtgggcct ggacatcgtg ctccacaccc aggactacta ctccaagacg
      481 ctctccaagg actacacccc gcgcaaggcc ctgaaggacc tggtggcttc cggcaacctg
      541 ggcaagaaga cgggcagggg catctacgac tggtccgcgg gcaggcccgc catcggggcc
      601 gacaacccca ccactgagta cgacttcacg cacatggtgg ccctgcaggt caacgaggct
      661 accaagtgcc tggaagaggg cgtaacgaac gaccccaagg acatcgacct cgccatcgcc
      721 aacggcggcg gcggcatggg cccgttcacg ctcgcccaga gcatcggcta cgacaaactc
      781 gtggcccgtt gcaacgaact ggcagacaag ttcgggatcg agaccttccg gcccacaaag
      841 accatgcagg aaggcaagat caaggtctag gcgtcgccaa cgacatccac cctgtcaccg
      901 gtgcaggggc tgccgcgcaa ggcagtccct gctcctttcc cgaagccccc tcgcaccctg
      961 gacttgtcac catgattgtg ggatagtacc gcgcatcccg tcaaccaaag gagggcattc
     1021 catgaaaggc tcgatcctcg tgttgggaac gctgatcacg ctcatcctct tcaccacggc
     1081 ggtattcgcc ggcaacccct ccaaggcgac acaggccgat gccaagaagg acaccctgat
     1141 cgtagaatcc cctgatgcac atggtcagga tatggaaagt ggaaagggcg cgaaccccaa
     1201 gccggacggt gccgactggg agaagaaaga caagggaaag accgtcacgg gcaccggctc
     1261 tcaggattcc aagaagggca aggatgccca ggagaggccg aagacccaac agggcgacga
     1321 taaaaagacc gataaggtga aggatgccaa ggacaaggac gttgccggca agccaggcca
     1381 tgacatggcc aggaaggaaa cggtggtggg ctatgtcatg accatcgaca acctgaccgg
     1441 cttgatcgtg gtcaaggaag agaacaagaa gacagagcgc gccatcgtgg tgaacgcgcg
     1501 cgacctcgcg tccctgtctg cgggcgccaa ggtgaacata gagctcgccc ccggcacgaa
     1561 caccgcggag aagatcagcg tcatcgccga gggcaagggc ggcgcaaaga gcaaaaggaa
     1621 gaagtagggg cccattgcct tggtgaacgg agaaggacac ggaggccttc cgccatggcc
     1681 ggcccccgtg tcctttcgct tcttgccggc tgatgcgccc ctgagaagaa ccgctgttct
     1741 tttgtagccc agcagacctc ctcaccccct cagggagttc aggggttctc ttccgattcc
     1801 atcgatggct tccagacctc ccagaccttg cccacgaggc ccggcccggg tttcagcgtg
     1861 agcttgcccg gcctccagcc ggacggcgtg gcctcggtgc ccttgcttgc ccgcacgtgc
     1921 tggaaggcct ggatctggcg gatggtctcg ccgacgttcc tgcccaccgg cggggtgagc
     1981 acctcgtagc cctgtatgac accgtccggg tcgatgatga agcggccgcg catctccacg
     2041 cccgcatccg ggtcgtacac gccgtagacg ctccccaccc ggccgccgcc gtccgagagc
     2101 atcgggaagg ggacgccgcc gtccaccatc ctggagagct cgtggtcgtt ccacatcttg
     2161 tgcacgaaca cgctgtccac gctcatggag aggacctcca cgccgagctt ctcgaactgg
     2221 gggtatttct cggcaactgc cgagacctcg gtcgctcaga cgaaggtgaa gtcacccggg
     2281 tagaagcaga gcaccaccca cttgccgagg tagtcggaga gcttcacctg cacgaacgtg
     2341 cccttgaagt acgcgggcgc cgtgaaatcc ggggccttgg cccccacctt gatcatgacc
     2401 tgtgcctcct tcctttcccc cgccagaacc gggggtgcct ccaccggctt ctcacccacc
     2461 ggcccgcctg tgggccgggc gcaccccgcc ttgatctctt caggcatagc ctttcccctt
     2521 tcccgtgtcc ccgtcccatg atgccggctt ttgccgaacg acctgtccgc cggcaccatt
     2581 ctcgattgtt atgagaataa tcaacgcatg cacgatggca atacccgaga caagacttcc
     2641 tagctcggcg cacccctcac ctcgagccgg gccggcactc gaccacgtgc aattccatgg
     2701 cactgcgcgg cggggcaggc gggtggtgcg tgcacgcacg gaatcatcca cacacccatc
     2761 agcgaggacc cggccggaga cggttggtgc gtacacgcag ggaatcatca cgtcaaagga
     2821 gggtatcgag cggtcttgcc tccccctgcc tgcgggcccc tcctgatccc cacggcaccc
     2881 acgggtgata tggcgcgggg tctcccctca cccccccctc tcgctccgat atccgcattc
     2941 cccccgcgtc gggcgatacg tacagagcct acaacctgca cgttgggcgc acggggagcc
     3001 cctccctcct ggatggcatc gggtgcgacc gggcagcggg gctgtgcacg ggaggcggtt
     3061 cggggcatgg ggtcgatcgt ggccaagcat cgggtgcggc ctggcgccag gggtgtttcc
     3121 cccctgtcag ccctgagacg gcctatcccc cgtcctcatc ctggtccttc gtgttcgtct
     3181 tcgccgtcat gctcatcctg tcgtccccga gacggcctag ctcgtcctca tcagcaggag
     3241 gctcaacgcc atcaccacca tgcccgccac gaggccgaga aggctgtcgt ggcccttgcc
     3301 gtacgccctg ctcgtgggga gcagctcgtc gaggctgatg tagaccatga tgcccgccac
     3361 gccgccgaag agcacgccca tcacctcggc ggggatcatc cctgacgcac ccccgacaaa
     3421 aaccataagc gcgagatacg cgatccccgc gcccacgggc tcggccaacc cgctcagcag
     3481 ggagtagaag aacgccttcc tcctgttccc ggtggcatag aagatgggga ccgagacgct
     3541 gatcccctcg ggtatgttgt gcagtgcgac tgccacggca atggccgccc ccagccccgg
     3601 gtcctggagg gcggccagga aggtggcaag accttccggg aaattgtgga tgccgatggc
     3661 cagggcagtg aatagcccca tgcgcatcag cttcctccca tgcggggtgt tctcctggag
     3721 gccctgagcc tgcgatgatg accccctcgg gcgaggcatc tgcgcggatg ggtcgtggag
     3781 caccatggtc tcggcctccg agtgcgtctc gtgcgggttg tcttgcgccg ggaccagggt
     3841 gtcgatcagg ccgatgagca gcatgccgga gaagaaggat gccgcattga tccagtgacc
     3901 ccagtcgccg taccgtccct cgagtgactc gaggcccttg aagaagatct ccacgaagga
     3961 gacgtagagc atcaccccgg ccgagaaccc c
//
LOCUS       DAOSCR010000205         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14255_length_3991_cov_3.105945, whole genome shotgun sequence.
ACCESSION   DAOSCR010000205 DAOSCR010000000
VERSION     DAOSCR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14255_length_3991_cov_3.105945"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..924)
                     /locus_tag="PLT69_06230"
     CDS             complement(271..924)
                     /locus_tag="PLT69_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ14071.1"
                     /translation="MTVLVIDDDEGECYLVEDILAEAGIKVDIAIGGEQAIRRLSERP
                     YPVVITDLRMPDIDGMAVIDFIRKRCMRSLIVVITGYVTIDNVIDALRAGAYDYIIKP
                     FSSELLLFTIRKAFDYIAMRDEQERLMYVDKLATLASATAHEVFQPLTVIIGEANAIL
                     KGSEEGSEQREMAGTILAEARKNRDIVRKLEGLSDYVTKTFPGGHTILDIDKGSSSG"
     gene            complement(1137..1325)
                     /locus_tag="PLT69_06235"
     CDS             complement(1137..1325)
                     /locus_tag="PLT69_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14072.1"
                     /translation="MRVEEYRERLLQRWEDVRPVVIYFAGERFVVEYDEHTNPLLSPA
                     VALVHSYLGNIELTRTDN"
     gene            complement(1407..2621)
                     /locus_tag="PLT69_06240"
     CDS             complement(1407..2621)
                     /locus_tag="PLT69_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14073.1"
                     /translation="MSSSMDIRTLARKIGIPFIDLFREAPDPRCMKLLKESFARQHAS
                     IPVRFLGDGVLIALGDPDFKDAVEQLSACIGRKVYPALADRAAIEAAIDRFYANGGST
                     LEEREPGEASSSRIVSVISNKGGVGKTHISINIARVLASFRKKVLLIDADLGNADISN
                     KLALFPEYTLYDFMLGDIDLASMIEKTPYGFDLIASSSGEFKLANINYVQRTKFIRGF
                     RKVSAPYEFAVFDLSAGISSTVLEFALASDDIVIVTTPQDIIAGYACIKAAFQHFKAI
                     EEKLMAKVEFYKPRRVFAPWVVMNQISDLNQGVFIFNRICQTADERINSLEEYFSVKP
                     QYLGGVVYDKEVVKNAETQRKPFTLVNPKSRPSQCIDYLGRMIMEPPELRSYSQEMKG
                     GLGRFAMIFGMG"
     gene            complement(2829..>3991)
                     /gene="mutS"
                     /locus_tag="PLT69_06245"
     CDS             complement(2829..>3991)
                     /gene="mutS"
                     /locus_tag="PLT69_06245"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ14074.1"
                     /translation="YIEIGKSQAAKAPDRYARRQTLVNAERFITPELKEYEAKLLGAQ
                     DAIAAIEQRIFEELRQSVIPHVPALKAAASSLALVDALASLAWAALKYGYCRPSICRD
                     RRIEVVGGRHPVVERVLKPGEYVPNPCAFDPDKDTIHLITGPNMSGKSTYMRQVALIC
                     IMAQIGSFVSAESARIGVVDRIFTRIGALDNLSAGQSTFLVEMTETADILNNATEKSL
                     VILDEIGRGTSTYDGMALAWAVAEYLNERRARTFFATHYHELADLSRRHKGVKNFHLA
                     VEESGAEVVFLRTLRRGAIGRSFGIYVARLAGIPREVIERARNLLEAVSSRARAVPSQ
                     ARQAPVQASLFEPSESALLQEILSVDLERMTPIEALNFLHRLKEKVSVPAER"
BASE COUNT          665 a         1238 c         1246 g          842 t
ORIGIN      
        1 gacagaagat acttgtcgga ggtcggttgc cttgcatgga cacggccgtg cataggagag
       61 ctcccccagg gccgggggaa aagcatgccg agggcgcctg cgcctcgacg tgaagcgggt
      121 cgaccgccgt ctcccctgcc ccgctcgagg cgctggatgc agggcctcgc tgacggccgg
      181 cccggtttcg cccggctcgt ggggcactga gaggtatcgc ccgcaccgag cgggaccgag
      241 gcggagtacg gcctgcccct cgggagatcc ctagcccgag gacgacccct tgtcgatgtc
      301 caggatcgtg tgcccgccag gaaaggtctt ggtcacgtag tcgctcaggc cttccagctt
      361 cctcacgatg tccctgttct ttctggcctc cgcgaggatg gtgcctgcca tctccctctg
      421 ctctgaaccc tcctcagagc ccttcaggat ggcgttggcc tccccgatga tgaccgtcag
      481 cggctggaag acctcgtggg ccgttgccga ggcgagggtc gcgagcttgt ccacgtacat
      541 cagccgttcc tgctcgtccc gcatggcgat gtagtcgaag gccttcctga tcgtgaacag
      601 gagcagctcg gagctgaaag gcttgatgat gtagtcgtac gcccctgcgc gcagcgcgtc
      661 gatgacgttg tctatggtga cgtaccccgt gatcaccacg atgagcgacc tcatgcaccg
      721 cttgcggatg aagtctatca cggccatgcc gtcgatgtcc ggcatcctca ggtctgtgat
      781 caccacgggg tatggtcttt cggaaaggcg ccttatggcc tgctcgccgc ctatggctat
      841 gtccaccttg atgccggcct cggcgaggat gtcctcgact aggtaacact ccccctcgtc
      901 gtcgtcgatg accaggacgg tcatgtcctg cgctggtttc tcgttgtcca tttctattcg
      961 aaaataaacg aggtgcggcc ccgaatcaag agccccgctc gagcaatggc gggaatcggg
     1021 agatgcacag ggcttggggt gcggcgaggc gtcaggagat gtacggggtc agggatgcca
     1081 tgaggtttcg cggaaacagg atgtccgggc cttcagcctg tgcggggcgt gcccggtcag
     1141 ttgtcggtcc tcgtgagctc gatgttcccg aggtacgagt gtacgagcgc gacggcgggg
     1201 ctgaggagcg ggtttgtatg ctcgtcgtac tcgaccacga accgttcccc ggcgaagtag
     1261 atcaccacgg gtctcacgtc ttcccagcgc tgcaagagtc tctccctgta ctcttccact
     1321 ctcatgcgtg tccgaccctt tctcggtctc tcttgttctt atctatcgga taagaccctg
     1381 tttccttgag gattacaggt ttgagatcag cccatcccga agatcatggc gaaccttccg
     1441 agacccccct tcatctcctg gctgtaggat ctcagctccg ggggttccat gatcatcctc
     1501 ccgaggtagt cgatacactg ggaagggcgg ctcttggggt tcacgagggt gaagggtttc
     1561 ctctgcgtct cggcgttctt gaccacttcc ttgtcgtaca ccaccccgcc gagatactgc
     1621 ggcttcacgg agaaatattc ctccaggctg tttatccgct cgtcagccgt ctggcatatc
     1681 ctgttgaaga tgaacactcc ctggttgagg tcggagatct ggttcatgac cacccacggc
     1741 gcgaagaccc ttcgcggctt gtagaactcg accttcgcca tgagcttttc ctcgatcgcc
     1801 ttgaagtgct ggaaggcggc cttgatgcag gcgtacccgg cgatgatgtc ctggggcgtg
     1861 gtgacgatca cgatgtcgtc ggacgccagg gcgaactcga gcaccgtgga actgatgccc
     1921 gcactcaaat cgaagacggc gaactcgtaa ggggcgctga cctttctgaa tccccgtatg
     1981 aacttggtac gctggacata gttgatgttg gcgagcttga actctcccga gctgctcgcg
     2041 atgagatcga acccgtaagg ggtcttctcg atcatgctcg cgaggtcgat gtcaccgagc
     2101 atgaagtcgt agagggtgta ttccgggaac agggcgagct tgttggatat gtccgcattc
     2161 ccgagatcgg cgtcgatgag cagtaccttc ttcctgaacg aggccaggac cctggcgatg
     2221 ttgatggata tgtgggtctt ccctaccccg cccttgttgg agatgaccga cacgatcctg
     2281 ctcgaggacg cctcccccgg ttccctctct tcaagggtcg agcccccgtt tgcatagaac
     2341 cgatcgatgg cggcctctat ggccgcccta tccgccagtg cggggtatac cttcctcccg
     2401 atgcatgcgc tcaactgttc gaccgcatcc ttgaaatccg ggtcgccgag ggcgatgagc
     2461 acaccgtcgc ccaggaaacg caccgggatg ctcgcgtgtt ggcgtgcgaa actctccttc
     2521 aagagcttca tgcacctggg atcaggggcc tccctgaaca ggtcgatgaa tgggatccct
     2581 atcttgcgtg caagtgtcct gatatccatg gaactcgaca tgtcagcctc gctgatagac
     2641 gttttcggcc gaagatctga aaagctttaa cctttcttca tggcggggtt cccgggtcgc
     2701 ctgccgccct cgggcgggcc ctggcgtgag gaggggaccg gcggtctcga tgtggccacg
     2761 ggcgggcgac caaggctgca gcacgccctc acggtcgatc ccatcgcatc cgcacggggg
     2821 cctggccttc aacgctcggc cggtacggac accttctcct tgaggcggtg gaggaaattg
     2881 agggcctcga tgggggtcat gcgctcgagg tcgaccgaga ggatctcttg gaggagcgcg
     2941 gactcggacg gttcgaagag gctggcctgg accggcgcct ggcgggcctg cgacggtacg
     3001 gccctggcac gcgagctcac cgcttccagc aggttcctcg cgcgctcgat cacctccctg
     3061 gggataccgg cgaggcgggc cacgtagata ccgaagcttc gtcctatggc ccctctcctc
     3121 aaggtcctga ggaagaccac ctcggccccg ctctcctcca ccgccaggtg gaagttcttc
     3181 acccccttgt ggcgcctgga gaggtctgcg agctcgtggt agtgcgtggc gaagaaggtc
     3241 ctggccctgc gctcgttcag gtactcggcg acggcccagg cgagcgccat cccgtcgtac
     3301 gtggatgtcc cgcggccgat ctcgtcgagg atcacgaggc tcttctcggt cgcgttgttg
     3361 aggatgtcgg ctgtctcggt catctcgacg aggaaggtgc tctggcccgc cgacaggttg
     3421 tccaacgccc ctatcctcgt gaagatcctg tccaccacgc cgatgcgggc gctttccgcg
     3481 ctcacgaacg agccgatctg cgccatgatg cagatgagcg ccacctggcg catgtaggtg
     3541 gactttcccg acatgttggg cccggttatg aggtggatgg tgtccttgtc ggggtcgaag
     3601 gcgcacgggt tcgggacgta ctcccctggc ttgagaacgc gttccacaac ggggtgacgc
     3661 cccccgacta cctctatcct cctgtcccga cagatcgagg gcctgcagta accgtacttg
     3721 agcgccgccc aggccagaga cgccagggcg tccacgagcg cgagggagga ggcggccgcc
     3781 ttcagcgcgg ggacatgggg gatcaccgac tgcctgagct cctcgaatat gcgctgctcg
     3841 atggcggcga tggcgtcctg cgcccccagg agtttcgcct cgtactcctt gagctccggc
     3901 gtgatgaaac gctcggcgtt caccagggtc tgccgcctgg cgtacctatc gggcgccttt
     3961 gccgcctgcg acttcccgat ctcgatgtag t
//
LOCUS       DAOSCR010000206         3976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14333_length_3976_cov_2.815863, whole genome shotgun sequence.
ACCESSION   DAOSCR010000206 DAOSCR010000000
VERSION     DAOSCR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14333_length_3976_cov_2.815863"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLT69_06250"
     CDS             complement(<1..985)
                     /locus_tag="PLT69_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14075.1"
                     /translation="MARDEDDPARLCESLTKKALKAADKGEADFARKLFEAALSHDPH
                     SPATLHHYGSFLFSQGKPADARDRLVQALEADPSNPEYHNDLAAVLHALGDTEKAIES
                     LEKALEADPEYAPALKNLTEVLLESGQIGKASETCQRLLSIHPDDAEASSLWNTVRKE
                     LGETEEPDLSHSSAHARARTFSRQRPEGVMCLMPFTTMTLSFLGVYNCACPDWIKMKI
                     GDLGASTIADTWNSEQARWIRAKMYEGKWEEICNWFCPHVITHEKTGRLHRYDELEAG
                     DLVTAQLAEEIRQGRTRLDSTPLVFKLDNSQTCNLSCIMCSRFLHREDPVML"
     gene            complement(986..2041)
                     /locus_tag="PLT69_06255"
                     /pseudo
     CDS             complement(986..2041)
                     /locus_tag="PLT69_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
     gene            complement(2363..3310)
                     /locus_tag="PLT69_06260"
                     /pseudo
     CDS             complement(2363..3310)
                     /locus_tag="PLT69_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          647 a         1176 c         1315 g          838 t
ORIGIN      
        1 cgagcatcac gggatcctcg cgatggagga accgggaaca catgatgcac gagaggttgc
       61 aggtctggga gttgtccagc ttgaacacga gcggggtgga gtccagtcgc gttctaccct
      121 gcctgatctc ctcggccagc tgcgcggtga cgagatcgcc cgcctcgagc tcatcgtagc
      181 gatgaagtct tccggtcttc tcatgggtga tcacgtgggg gcagaaccag ttgcagatct
      241 cctcccactt cccctcgtac atcttggccc ggatccatcg ggcctgctcg ctgttccagg
      301 tgtccgcgat ggtggaggcc ccaagatccc cgatcttcat cttgatccag tccggacagg
      361 cgcagttgta cacccccagg aacgagaggg tcatggtggt gaacggcatc aggcacatga
      421 cgccttcggg tctctgccgg gagaaggttc gtgcacgtgc gtgcgcagag gaatgagaaa
      481 gatcgggttc ttcagtctca ccgagctcct ttcggaccgt gttccacagc gatgacgcct
      541 ccgcgtcgtc tggatggatc gacagcagtc tctggcatgt ctcgctggcc ttcccgatct
      601 gaccgctctc caagaggacc tcggtgaggt tcttcagggc gggtgcgtat tcggggtcgg
      661 cctcgagggc cttctcgagg ctctcgatgg ccttctctgt atcgcccagg gcatggagca
      721 ccgcggcgag gtcgttgtgg tactcggggt tggaggggtc ggcctcgagc gcctggacga
      781 gcctgtctct cgcatccgca ggttttccct ggctgaagag gaaggacccg tagtggtgga
      841 gggtggcggg cgagtgcggg tcgtgggaca gtgcggcctc gaagagcttc cgggcgaagt
      901 cggcctcgcc cttgtcggca gccttcaggg ccttcttcgt gaggctctcg cagagccttg
      961 cggggtcatc ctcgtcgcgc gccacgcgcc gcgtcgtctc caggtaggcc gtgccgtgct
     1021 tgaggtccgg ttcgaggtgg ttgccctccg cccactcgtt ccaggcgttg atgaacacga
     1081 tcctctcttc gggggcgttg gcgcacgcct tgcggaccgc ggactccagc cacttcccgt
     1141 agagttccgg ggtcgacccg tggaggacga ccccgtcctt tccccgtctc ggcgtgttgt
     1201 cccaggacgg ggtcacgcac gggaagcgca ggtatccggg ctggggcttg ctcatcatcc
     1261 tttgcacgac gtccccgtag tcgtagacgt ggaggtcagg gtacgtgtcg tccttcaggc
     1321 ccagcatcga ccagtctggc tggaactcca ccgccgcgtc gaagccgagc tccgcaggcg
     1381 gcgtctgctc gtcggcgaag ctctccaccc tgcagaggta caggtcggga aacccggccc
     1441 tcttggcctc cttcctccac agggcggccg ttctcgacgg gtcaggcagg cctcccgccc
     1501 ggtagacgag aaagagcggc ctgccgtgta tcttgatgta cctggggtcc ttgaacgcag
     1561 cgaggagcca cctgatgtga gcaaggtcgt cctcctcgcc gtagtcctgc ctcatgagga
     1621 tcttcttgtc cccgccgtcc cagaccctgg tccagttctc gttggcccag cacaggcaga
     1681 aggggaagtc cggcctgccc gaggcgagca cctcgtcgaa gaccctgttc aggaggcgcc
     1741 tgcccttgaa ccagtagtgg tagtagcaga agcccgagat gccgtggctg cgcgcgagct
     1801 cggcctgggc ttccctggtc tccgcgagtc tgaggtcgta gaacccgagg tctgccggga
     1861 tatgtggctg gtagtggcct tcgaacagcg gccttgcctt ggccacgttg gtccattccg
     1921 tgaatccctt gccccaccag cgatcgtttt cggggatcgg atggtactgg gggaggtaga
     1981 aggcgaggaa tcgaacggcc cggtctgcgg gcgccggctg gtcttttcgg ggtttctcca
     2041 tcgaagtgct ctccgtggtt tctggtgtgg cgactgggcg tcgttttccc aatgtcttcg
     2101 atgcatcctg cgtttccggc agtgcgctgc gcccgtcctg caggagatcc atggctggtg
     2161 tcatgagggc gttggtgcat gcccgggtat tacggaacct gttctggatc aggtcatccg
     2221 aagactctga acctgtgtgt gcacagtgga tgggtgatgg ggatgagtcc ttcgcgacgc
     2281 ctttgatgcg ctcgagggcc acctgggcat cggtgtcgcc ggggttctgc cgcaggactt
     2341 cggtatactg tctttcagct tccttcaagc gtccctgcgc cgccaggagg tccgcgaggt
     2401 ttctgcgcgc gtccagcagg gtgcgatcga gattgaccgc cctgtaaaag agggtgacgg
     2461 ccctgtcatg gtcgccgttc aggtagcaga gcgtgccgag gtcattggtc agttcggcgt
     2521 cgcccggcgc cagggcgacg gccttctgca ggagttccac ggcctgcctg aggtctccct
     2581 gccggtgcct gagcagggcg agctggtgga gtgcacgggg gtccgcaggg tcccgggaca
     2641 gggccttgtc cagaagtcct tgagcatctt ccaggcgccc ttctgcaagg gcagatcggg
     2701 cctgtaatac gtatgctttc ccgggcttct ttcctgcaac ggatggcgat cgttttctgc
     2761 gggtcgtgag catccttgcc atccggacct tcctctttcc cgacacatgg gcatagaggt
     2821 cacgataggt ttccaggaga gcatcgatct cttcatccag accgggtatc tgcgtctgca
     2881 tcgctccccg cgcgaggtcg tccacgaggg aaggggtgtt gatcagatcg agcatctttc
     2941 cggcgagtgc atcggcgtca cccacgggga tccggaaccc gttgatgccg tcctggacga
     3001 tgaatgcacc gaagaagtcg gtggcgatga caggcacacc caagtagatg aactccctga
     3061 gtacccggtt gtaggtatcg aaataggagg ggacgatgca gacgtctatg gctgcggcaa
     3121 tggaggggag atcgccatcg gtgaaggccc cttgatagat gaatgactca gggtgtgccg
     3181 actggaggcg atccagccat tgttggtact cggggttgac gatctggccg tacacgtgga
     3241 agacgaagcc gcctctgccg cagacccgtt cgaaggcgcg cagggctacg tcgatcccct
     3301 tgctgaacat gatgttgccg acaaaggcga agtgaaggat cccgccggga cggctgttcc
     3361 gtgccgtggg cctgggaaat gcgaaatcga acccccgggg gatcacccgt gttttctcgt
     3421 gggggatggg tatgaaacgg gtaaagtagt ccctgaacgg cgcggaggtg aagacgacca
     3481 tgtccacggc atcccggtac agcctccgga cataggcgaa gcggtcctcg atctttttcc
     3541 ccaccgggtg ggactggttt tggggcgcga ggttgcgcag ggcggcttca gggagcgttg
     3601 cgaggcagtc ggcgcacgtg cccgggttct tcggtcccaa gcagagcctt ccgtccagac
     3661 ccgaggagag gaccgggtag tcctggtggc acagttcctc gcagttgcac acgttcctga
     3721 caacgatgca tcctcttctg gcaaagatct ccacgatctc cgccggcatg tacacgtagt
     3781 cgttgacctg cacgacttgc ggacagaagt cgtcgatgac gtcctcgatg atttttcgat
     3841 accaggcggt tgcatcctca ccgggcgctg cgcaataggc atcgaatttg ttcatggcag
     3901 ggaccctgta cagggtgaac ccctcgcggg gctccctgct gaagttgtcc tcgagctcgg
     3961 gcgccggtat gagaac
//
LOCUS       DAOSCR010000207         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14373_length_3967_cov_2.875256, whole genome shotgun sequence.
ACCESSION   DAOSCR010000207 DAOSCR010000000
VERSION     DAOSCR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14373_length_3967_cov_2.875256"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PLT69_06265"
     CDS             complement(<1..1271)
                     /locus_tag="PLT69_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ14076.1"
                     /translation="MASSYRLTCLFVDLKTATSRRVVLGEDVSMARLGGRGIGVHFLA
                     AAPTIAPLHEDNPLVFATGPLVGSRIPSSGRMVISSFSPLTNTVCTSVTGGDFAYGMR
                     KAGIDVIHLSGKAPVRSVVFIEDDRVSMEKDPFPDDVGLELVFERLSSPGRSTAAIGE
                     AAIKGCLYSSIMVDGCFASGRGGLGLVMASKNVRAVSIRGSGPSRRIPVDEHEEDRAR
                     SDIVRLFDASPFVRGRSGIAASGTAALIDLVAERRMMPTANFRRTYFEGYRAFSANAF
                     ARTFSPRRHACRGCPIACKGKAAGPIPEYETIAHFGALNENDDIASIVEANRLCNSLG
                     MDTISAASTIACLAEIRGVRFQGEGLVSMVRRIVHAGTSGELLRQGSLRMARALGAPE
                     TSMSVKGLELPAYDPRGAYGISEPGRQRPEL"
     gene            1500..2327
                     /locus_tag="PLT69_06270"
     CDS             1500..2327
                     /locus_tag="PLT69_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14077.1"
                     /translation="MYLPERTEGYAAERSSLVLYALSSMLLLVCGWLFLSYRIVPVNR
                     DAGYFVSVAREILGGLTPTAGVDTAYTPFVYYVYAVWMKVVPGTWENLVLLVYLVHVL
                     NAVLVGLVCARMSVRKAACLIVSTAYFSSVIVCEGYGICLEPFQVTFALVALLALLVV
                     RRSPAAYAFSGLALGVSIMFKQYSVFMLAGFAASLVAGFPGEARREMSLRARLGGIVV
                     MIAASVVPFFAFVSLTEATCVDALVAFGFLGHRATGYALDTSFGLHQRLAYLYDNAT"
     gene            2311..3309
                     /locus_tag="PLT69_06275"
     CDS             2311..3309
                     /locus_tag="PLT69_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14078.1"
                     /translation="MTTRPRLFLPALAYAVVGRDKDDTMGSARIPAMVLAFSALPLAV
                     RWYKHYFQLVAPWSFILAGMLLESALREPFAKGRPGRLLAWITVCVVMVLVPVFAEIR
                     PSFRQFSPRELFRVALVSAFFLWAFRFRLLASAKAWVPAVLALVLGECLLCTAEIPFG
                     ELEQQKRVQLVEAVRMMRVLGWGSEVIVVDIPHLYVLCGYRHPGGDYGFILPTTVGEK
                     MGKRVLSRTENVILKAGHPLDGSGYLERHGFKRVGVVAGTDIVRYEKPLGDVPGAGRD
                     RARRSEQDGRILHESPAGEPCRAFISLSGVISSMRKGWPYNGEPSFRMYGHAETMI"
     gene            3366..>3967
                     /locus_tag="PLT69_06280"
     CDS             3366..>3967
                     /locus_tag="PLT69_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ14079.1"
                     /translation="MRILLVEDDLRTAGLIRKMLTQEGYIVEHAIDGEDGLILALNEN
                     FDAAILDIMLPRLDGIQLIERIRGHGRILPVIFLSARSSVDDRIKGLRAGGDDYLVKP
                     FALSELLTRVQALIRRATSMSEPTTLKVGDLTMDLLSRKVSRSNMTIELQPLEFSLLE
                     YLMRNAGRVVSKTMIIEHVWDYSFDPHTNVVEARICHLRD"
BASE COUNT          671 a         1213 c         1294 g          789 t
ORIGIN      
        1 ctcagctccg gtcgctgccg gccaggctcg ctgatgccgt aggcgcccct ggggtcgtag
       61 gcggggagct ccaggccctt gacgctcatg gacgtctcgg gcgcgccgag tgcccgtgcc
      121 atcctgagcg acccctgcct gaggagttcc cccgaggtgc cggcatgcac gatccggcgg
      181 accatcgaga cgaggccttc gccttgaaac ctcaccccgc ggatctccgc caggcaggct
      241 atggtggacg cggcgcttat ggtgtccatg cccagcgaat tgcagagcct gttcgcttcc
      301 acgatggatg cgatatcgtc gttctcgttg agcgccccga agtgggcgat ggtctcgtac
      361 tcggggatgg ggccggccgc tttccccttg cacgcgatgg ggcacccgcg gcatgcgtgg
      421 cgcctgggtg agaacgtcct cgcaaaggcg ttcgcagaga aggcccggta tccctcgaag
      481 taggtcctcc ggaagttggc ggtgggcatc atcctccgct cggcgacgag gtcgatgagt
      541 gcggccgtcc ccgatgctgc tatgccggac cgccccctca cgaacgggga ggcgtcgaac
      601 aggcggacga tgtcggaacg ggcccggtct tcctcatgct cgtccacggg gatcctcctg
      661 gagggcccgc tccccctgat cgagaccgcc ctcacgttct tggaggccat gaccagtccg
      721 agtccgcccc tgccggaggc gaagcaaccg tcgaccatga tggacgagta caggcacccc
      781 ttgatggcgg cctcgccgat ggccgcggtg gagcggcccg gggacgataa ccgctcgaac
      841 accagttcga ggccaacatc gtcggggaag gggtctttct ccatgctcac tcggtcgtcc
      901 tcgatgaaca ccacgctgcg cacgggggcc ttgcccgaga ggtggatcac gtcgatgcct
      961 gcctttctca tcccgtaggc gaagtcgccg cccgtgacgg aggtgcacac cgtgttggtc
     1021 aggggggaga acgacgagat caccatgcgg cccgacgacg gtatcctgga acccacgagc
     1081 gggcctgttg cgaacacgag ggggttgtcc tcgtgcaacg gggcgatcgt gggagcggcc
     1141 gcgaggaagt ggacgccgat ccccctgccg ccgagccggg ccatggacac gtcttccccg
     1201 aggaccacac gccggctcgt cgccgtcttc aggtccacga agaggcacgt gagcctgtag
     1261 gaggaagcca tgaaccgtta ctgtatagga ttcggcatct cggggcaatc gcggtgagca
     1321 acaccgccct tcgggtccgg caggggtgcc cgggaagatt tctcactaca ggctggagcg
     1381 tggagacggg gatcggaggt ccagggtcct gttcccctgc caaagagccg gccttgaagg
     1441 cccctaaggc gtatcttcgc tgttggccgg gcttgcgtgg tgatggtaca gtgctcccca
     1501 tgtatctccc ggagagaacc gaagggtatg cggcggagag atcgtctctc gttctgtatg
     1561 cgctttcctc gatgctcctg ctcgtgtgcg ggtggctctt cctgtcgtac cggatcgtgc
     1621 ccgtgaacag ggacgccggc tacttcgtga gcgtggccag ggagatactc gggggtctca
     1681 cccccaccgc gggggtggac acggcctaca cgccctttgt ctactacgtc tatgcagtct
     1741 ggatgaaggt ggtgccgggg acgtgggaga acctcgtgct cctggtctac ctggtccatg
     1801 tcctcaatgc ggtgcttgtg ggtcttgtct gcgcgaggat gtccgtccgg aaggcggcgt
     1861 gcctcatcgt gtccacggca tacttctcgt cggtcatcgt ctgcgaggga tacggtatct
     1921 gtctggaacc cttccaggtg accttcgcgc ttgtcgccct cttggccctg ctggttgtcc
     1981 ggagatcgcc ggccgcatac gccttctcgg ggctggcgct cggcgtctcg atcatgttca
     2041 agcagtattc ggtcttcatg ctcgcgggat tcgccgcatc gctcgtcgcc ggattccctg
     2101 gggaggctcg gcgggaaatg agccttcgtg cgaggctcgg cggcatcgtc gtgatgatcg
     2161 ccgcctctgt cgtgcctttc ttcgccttcg tgtccctcac cgaggcgacg tgtgtggatg
     2221 cgctcgtggc gttcgggttt ttaggacaca gggcgacggg ctacgctctg gatacgtcct
     2281 tcgggctgca ccagagactc gcatacctgt atgacaacgc gacctaggct gttcctgccc
     2341 gccctggcct acgcagtcgt cgggcgcgac aaggacgaca cgatgggctc tgcccggatc
     2401 ccggcgatgg tgcttgcgtt ctccgcactc cccctggctg tcaggtggta caagcactat
     2461 ttccagctcg tggcgccatg gagcttcatc ctcgcgggga tgctcctcga gtcggccctc
     2521 cgggaaccct tcgccaaggg caggccgggc agactcctcg catggatcac ggtatgcgtc
     2581 gtcatggtgc tcgtccctgt ctttgccgag atcaggccgt ccttcaggca gttctcgccg
     2641 cgggaactgt tcagggtggc gctcgtctcg gcgttcttcc tctgggcctt caggttcagg
     2701 ctgctcgcct cggcgaaggc atgggtcccc gcggtcctag cgctcgtctt gggggagtgc
     2761 ctgctgtgca cggcagagat ccccttcggg gaactcgagc agcaaaagag agtgcagctt
     2821 gtggaggccg tgcggatgat gcgggtgctc gggtggggaa gcgaggtcat cgttgtcgat
     2881 atcccgcacc tctacgtcct ctgtggctat cggcacccgg gtggggacta cggattcatt
     2941 ttaccgacca ctgtgggcga aaagatggga aaaagggtcc tctcgaggac cgagaacgtg
     3001 atcctcaagg cgggccatcc cttggacggg tcggggtatc tcgagagaca cgggttcaag
     3061 agggtgggcg tggtggcggg aaccgacatc gttcgctacg agaagcccct gggggatgtc
     3121 ccgggtgcgg ggcgtgaccg agcaagacgg tctgagcagg atggacgcat actccacgaa
     3181 agccctgccg gtgagccctg cagggctttt atctcattat caggtgtgat atcctcgatg
     3241 agaaagggat ggccatacaa tggtgagccc agctttcgga tgtatggaca cgcggagacg
     3301 atgatctaat gggtaggcca cgttattgcc ggatgtgctg tcgcacagga tagagggaca
     3361 cggacatgag aatactcctg gttgaagatg atctccggac agccggcctc atccggaaga
     3421 tgctcaccca ggaagggtac atcgtggaac atgccattga cggggaagac gggctcattc
     3481 tggccctcaa cgagaacttc gacgcggcga tcctcgatat catgctcccc aggctggatg
     3541 gcatacaact tatcgaaaga atacgtgggc acggcagaat actccctgtc atattcctca
     3601 gcgcccggag ttcggtggat gaccgtataa aaggactcag ggcgggcggg gatgactacc
     3661 tggtcaaacc cttcgccctt tccgagctcc tgacccgcgt ccaggccctg atccgccgtg
     3721 ccacctccat gagcgaacca acgacgctga aggtggggga tctcaccatg gacctcctct
     3781 cgcggaaggt atcccggtcc aacatgacga tcgaactcca gcccctggaa ttctccctcc
     3841 tcgaatacct catgcggaat gccggtcggg tcgtgtccaa gaccatgatc atcgagcatg
     3901 tctgggacta cagcttcgac ccgcacacca acgtggtgga ggcccgcatc tgccatctca
     3961 gggacaa
//
LOCUS       DAOSCR010000208         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14392_length_3959_cov_3.335041, whole genome shotgun sequence.
ACCESSION   DAOSCR010000208 DAOSCR010000000
VERSION     DAOSCR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14392_length_3959_cov_3.335041"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLT69_06285"
     CDS             complement(<1..996)
                     /locus_tag="PLT69_06285"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ14080.1"
                     /translation="MKIRCTACGRAYEIPDERLKAFSGTLTLPCPSCKAPITVELPQD
                     TGGHAMEGGRERLEGDALKERILKTMKDLPAMPQVAQKAREVVANPSSSFKDLAMVIE
                     TDQAIATRILRIANSPFYGLSGKISSVQHASVILGTKTLMEILNLACSSEILGQRLPG
                     YDLDAGELWKHSLAVAAASRIIALKVDKALEQDAFSAGLIHDAGKLILDPHILERKEE
                     FKAFVERGDKTFLSAEQGILGFDHARLAWDACESWRIPEHIARAVGCHHDPTPSNGDL
                     LCFVVHMADAVAMMSGIGAGLDGLLYTVHPDAARMLGLTPDDIGEIMGEIADYVDK"
     gene            complement(1019..1456)
                     /locus_tag="PLT69_06290"
     CDS             complement(1019..1456)
                     /locus_tag="PLT69_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14081.1"
                     /translation="MKTTAVKPMKCEFADEGMLWIDKIATGVGVILYNPEYRCAVGFH
                     VLRGKSTGAKVDNPGYFADTALEHAVKEFEQRGIPLKVSVAIAGGATLLSGTHQQEVG
                     TVLVNQVKEILAARKLDIKLESVGGTKMRTMILNIDEGKIKIT"
     gene            complement(1837..2385)
                     /locus_tag="PLT69_06295"
     CDS             complement(1837..2385)
                     /locus_tag="PLT69_06295"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOJ14082.1"
                     /translation="MPGETLFPASFWTEYTVGCPADRVRVMTPVGWLIDIYHGVLRFS
                     DGHPIVVAWLSAASLVTFLGTLVLIPVMVARMPADYFLYDRSEIARYRKMHPAWSLLT
                     VVVKNLLGVVFIACGIAMLVLPGQGVLTILVGITLVSFPKKRSLELMLIRRKSVRSAV
                     DWVRAKAGREPIVLPGNDRGRS"
     gene            3736..>3959
                     /locus_tag="PLT69_06300"
     CDS             3736..>3959
                     /locus_tag="PLT69_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14083.1"
                     /translation="MRRLVVFFTMVFMGITAMVFLPQADLDAASLLVTWNRNPETDIA
                     GYRVYYGTRSGAFDHVAKAGNATSHTIQNVA"
BASE COUNT          692 a         1352 c         1166 g          749 t
ORIGIN      
        1 cttgtccacg taatcggcga tctcacccat gatctcgccg atgtcgtcag gggtaagccc
       61 gagcatccgg gcggcatccg ggtgcaccgt gtagagcagc ccgtcgaggc cggcacctat
      121 gccgctcatc atggcgacgg cgtccgccat gtgtacgacg aagcagagga ggtcgccgtt
      181 ggagggtgtc gggtcgtggt gacaacccac tgcccgggcg atgtgttcag gtatgcgcca
      241 gctctcgcag gcatcccagg cgagcctcgc gtggtcgaac ccgaggatgc cctgctcggc
      301 gctcaggaac gtcttgtcac ccctctcgac gaaggccttg aactcttcct tcctctcgag
      361 gatgtggggg tcgaggatga gtttgccagc atcgtgtatc aggccggcgg agaacgcgtc
      421 ctgctcgagg gccttgtcca ccttgagagc tatgatcctg gaggctgccg cgaccgccag
      481 ggaatgcttc cagagctcgc ccgcgtccag gtcgtagccg ggcagcctct gcccgaggat
      541 ctcggaggag catgccaggt tcagtatctc catgagggtc ttcgtgccga ggatcaccga
      601 tgcgtgctgc acggatgaga tcttcccgga aaggccgtag aagggcgagt tggcgatcct
      661 gaggatccgg gtggcgatgg cctggtccgt ctcgataacc atggccaggt ccttgaagct
      721 ggaggacggg ttggccacca cctcccgcgc cttctgtgcc acctggggca tggccgggag
      781 atccttcatg gtcttcagga tgcgttcctt gagcgcgtct ccctcgaggc gctccctgcc
      841 cccctccatc gcatgcccgc cggtatcctg tgggagctcg accgtgatgg gcgccttgca
      901 gctggggcac ggcagggtta gggttcctga aaaggccttc aggcgttcgt cggggatctc
      961 gtatgctctt ccacaggccg tacaccggat cttcatgggg cttcctccag ggctcgattc
     1021 acgtgatctt gatcttcccc tcgtctatgt tcaggatcat ggttctcatt ttcgtcccac
     1081 ccacgctctc gagcttgatg tccagcttgc gcgcggcgag gatctccttg acctggttca
     1141 ccaagacggt acccacttcc tgctggtgtg tgcccgacaa cagcgtcgcc ccgcctgcga
     1201 tcgccacgga gaccttgagc ggtatgcccc tctgctcgaa ctctttcacg gcgtgctcga
     1261 gcgcggtgtc agcgaaatac ccggggttgt cgaccttcgc gccggtcgac ttccccctga
     1321 ggacgtggaa acccaccgca catcggtatt ccgggttgta gaggatgaca ccgacgccgg
     1381 ttgcgatctt gtctatccac agcatgccct cgtcggcaaa ctcgcatttc atcggtttga
     1441 ccgcagtggt cttcattaat ctctctcacc ttccctgtcc gaatcggtcc gtatccaatg
     1501 atcaaaagat tggacctatg gcctaccaca atccataccc gaagacaaac cccgcaggac
     1561 catgatgcgt cacggtcgtc ctcggccctc ccctcgacct ggtctgtgga tcgtctcgcg
     1621 agagaactgc aggacagccc ccgcacgctt tccgcctgtg ctccggtacc gggggatcct
     1681 gcccttgccg tgacgctgcc ccgaactaac cctctggttg ccggatcgta cggggatggg
     1741 gggtggcaca tcccccttga agcccgcctg cacctggtcc ggggtcgtac atgcgctgga
     1801 ggccgccctt gcaggaccct tgagcaggcc aggtcttcac gatcggccgc ggtcgttgcc
     1861 cggcagcacg atcggctctc tgcccgcctt cgcccgtacc cagtcgacgg cggagcgcac
     1921 cgacttcctc ctgatcagca tgagctcgag ggagcgcttc ttcggaaagc tcacgagggt
     1981 tatccccacc aggatggtga gcaccccctg gccgggcaag acgagcatcg ctatgccgca
     2041 ggcgatgaag accacgccca ggaggttctt gaccaccacg gtcagcaggc tccaggcagg
     2101 gtgcatcttc cggtacctcg cgatctcgga ccggtcgtag aggaagtagt cggcgggcat
     2161 cctcgccacc atcaccggga tcaagaccaa ggtgccgaga aaggtgacca aggacgccgc
     2221 ggagagccac gccacgacga tggggtgccc gtcggagaac cgcagtactc cgtggtagat
     2281 atcgatgagc cagccgacgg gcgtcatcac gcgcacccgg tccgccggac agcccaccgt
     2341 atactcggtc cagaaagacg ctggaaagag ggtctcaccc ggcatccggg cgctgtccca
     2401 tgtccagggc acattttaag aaccaatccc ccctcgtcca cttccatcct tgggtctcgc
     2461 atgatcgccc cacggtcctt gtccagatga cccgtcacgg ccgggcctag ggcatccttt
     2521 cggcagaccg cccggcccat tctccatcaa cctgcccggt gactcacggt gcgaggggtt
     2581 cactggagcc cggccgctct ccccaccacc ccggcccctg tccagctccg gggggccccc
     2641 tttcggccgc cttttctcag gcggcggatt ctccatggcc tgggccggct ttcccgagac
     2701 ggttgcttga ccatcttcca ggacagcccg gggcatgtca agaccgcatg cggctttgag
     2761 accgacaaac cacgatcgaa cctccggcga cgccctggcg gggaggggct gtgtgccagg
     2821 ccctcgtcgg cgggacaccc aggcagccac cggctgcccc ctcacaccct taaagaagct
     2881 cgagcgagcc cggaccgcca caccagccac gccttgacgg ctcatgacga ctcaccatcg
     2941 cggaccaccg actgggaaat acctgcatag ggatgtggct gatttgacgg ctcatgacga
     3001 ctcaccatcg cgaaccaccc gcgtgtgtcc tcagggacat gcctttcgtt tccatgcgac
     3061 gttcacaccg ccggtgatgc atcgaggcga aagcgggcct aacggaaggg gtggggcagg
     3121 tcaggcgccg agacgcccga ggtgaaccga ccgctaccgg cggcggggca cggcccggcc
     3181 tggtcgggcc ttgggagttt catgccgcag cgccccctta cgtcacccgc catgcatgcg
     3241 cgaccgttca ccctgtacct gcgccccgat ccaggcgccg atcacagcgt gcccgacatc
     3301 ccaagcccgg catccctcgg caggagacac ccgcacgcgt gatccggctt ggttgcatcc
     3361 gcgcacccag ggacttacgt ctttccccgc attgtggtgt gcccagaatc atggtgcgtc
     3421 cgcacaccca gggacgtgcg cccgtcaccg gctccccggg aaggacaccc cccacggcac
     3481 tccccacaga agaccggccc ctggcacgtg cgtggcctgc acagacgctc gaggctcaac
     3541 gtcgtatagg cgcacggtgc atcgctgccc ttccgagatc ccctcacccc ggttacacta
     3601 tcagcacacg cgtgaaccaa aatcgtaacc gagcgtttcc atcttgaacc tcaacacccc
     3661 gatatccgtt tcttttttgt aatggcccga aagttgccgg tgcttgggca tcctgcaccc
     3721 atgcgaggta tccgcttgag aagactcgtt gtgtttttca cgatggtatt catgggcata
     3781 accgcgatgg ttttccttcc tcaggcagac cttgacgccg catccctcct ggtgacctgg
     3841 aaccggaacc cggagaccga catcgccggg taccgcgtct actacgggac acgaagcggg
     3901 gcgttcgatc atgtcgccaa agccgggaac gccacgagcc acacgatcca gaacgtggc
//
LOCUS       DAOSCR010000209         3952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14440_length_3952_cov_2.597896, whole genome shotgun sequence.
ACCESSION   DAOSCR010000209 DAOSCR010000000
VERSION     DAOSCR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14440_length_3952_cov_2.597896"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PLT69_06305"
     CDS             <1..954
                     /locus_tag="PLT69_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14084.1"
                     /translation="DGEGGAILVWIEGWGNNLAVQRIDSDGNLLWNGGLPVYLGEDAY
                     CPRLVTASDGGAVVIWVSTDNSTIFAQKVGLDGEPLWNGGDGLVLDASMGWINGAFID
                     LASDEEGGAFVTWYDWPDGMNGSGFAQHVDASGVKTWGSPVMFTSLDGFREVSQHPRK
                     TVEDGRGGFIAAWEGPDECIMAQRVDKHGTLLWGDGGVVVSYGESIEYGPRLASGGPG
                     IPGGAIAVWVDPRVTVKAPDSQNVYMQNINADGSLGVLPGEEEEDSYEGVSSKGDDDD
                     RIMGVKCFIESASPGSHAPAVLSAVLLLSALIGVSLRRKPD"
     gene            1871..3073
                     /locus_tag="PLT69_06310"
     CDS             1871..3073
                     /locus_tag="PLT69_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ14085.1"
                     /translation="MERRDFLKALAGVSVAAACPLGSRARAARCAPDRQAALKKGADQ
                     KVYLAGVKAGASDEELKVAVRQAALAATDFSWLSRGDTVFVKPAVNSSNPYPATTCPA
                     ALAAMVELLKDKGAGRVICGDMSGVEHVRFTREGISGSSRRLMESCGIAGAALAAGAE
                     LHFFEEQGWDAFHEEAPAEGSHWKHGLMMPDILREVQHIVLMPRCGSHVLAGSTLGMK
                     AAVGYWRHDTRLEYHRDAATFHQKTAEGNTVESLRTKQRLVVTCGHKVLATFGPDEGY
                     VMEPQNGLVIASESIVAHDMVSLAWLLENRRLMPDSEKGSFMDTSPFVAWIGNRMVVT
                     WLGGLGQGILSDGFAKQPADSVWDDPVLARAYELFGGVPGVVLETANAAVDDGLKKRL
                     EEMTALPS"
     gene            3255..3683
                     /locus_tag="PLT69_06315"
     CDS             3255..3683
                     /locus_tag="PLT69_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007548223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HOJ14086.1"
                     /translation="MRNRTLAALVCLHMVFLATSPAFASLVPSVDSQGNTHRSIEDMK
                     TIEKALEMKIVQEKLRAYGLDAQEVKAKLSSMSPTQIHTLARACDDVLAGGDSGLGVV
                     IALLVIVILVIIILKLLNKEIIIRMSDRDGPAVHTGHPVC"
     gene            3643..>3952
                     /locus_tag="PLT69_06320"
     CDS             3643..>3952
                     /locus_tag="PLT69_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOJ14087.1"
                     /translation="MTALLSILAILFAEAPQAFVDGVPFIRQDPRLCGPAALASVLAY
                     YGLAVDQKAIADFAYTEKLGGALMTDLENYARSLGCRTHLAQGTSEDLIDALGKGRPV
                     V"
BASE COUNT          708 a         1271 c         1340 g          633 t
ORIGIN      
        1 gacggcgaag gcggcgccat cctcgtgtgg atagagggct ggggcaacaa cctggctgtc
       61 cagcgcatcg acagcgacgg caacctgctc tggaacggcg gcctcccggt gtacctggga
      121 gaagacgcct actgccccag gctggtgacc gccagcgacg gcggcgcggt cgtcatttgg
      181 gtaagcactg acaatagcac catctttgcc caaaaggtgg gcctggacgg cgagcctctc
      241 tggaacgggg gggatgggtt agtgcttgac gcatcgatgg ggtggatcaa cggcgcattc
      301 atcgacctcg catcggacga ggaaggtggg gccttcgtga cctggtatga ctggcctgac
      361 ggaatgaatg ggtccgggtt tgcccagcac gtggacgcat ccggggtgaa gacgtggggc
      421 tccccggtga tgttcacctc tcttgatggt ttcagagagg tatcacagca cccacgcaag
      481 accgtggagg acggaagggg cgggttcatc gccgcgtggg aaggccctga cgagtgcatc
      541 atggcacagc gcgtggacaa gcacggcacg ctcctgtggg gggatggggg cgtggtcgtc
      601 tcctatggcg aaagcataga atacgggccc cgcctggcct ccgggggccc agggataccg
      661 gggggagcga tcgccgtgtg ggtcgacccc cgggtcacgg tcaaggctcc agactcccag
      721 aatgtctaca tgcagaacat caacgccgac ggatcgctgg gggtgctccc tggagaagag
      781 gaggaggact cctacgaggg cgtctcctcc aagggagacg atgacgacag gatcatgggc
      841 gtcaagtgct tcatcgagtc ggcttccccg ggatcgcacg cgcctgcggt cttgtcggcc
      901 gtcctcctgc tctcggcgct tatcggcgta tctctcagga gaaagcctga ctgatcccga
      961 ttgtctctcc aagacgtgaa gggggcgggg ttcccgtccc cttcacgtct ttccagcgag
     1021 agcggtacac tacagacaac ctcgctttca gggtatggtc cggttccgtc tcgagcacgc
     1081 ccttggtgac gcgacgctat ccctcaccac gatccgggat accaggtggt cctgccccac
     1141 aggccgaccc ggccctgggc atgatggctg cgcctgtcac cccggatgcc tgcaccgcag
     1201 ctcaaggcac gaggccaggg cgtgatgggc cttgaagggg catgcccgtc ccccttgtct
     1261 cccggtcgcg atcggttgtt gaaagcaagt ccgccccacg gtatgatcgc ctgtgactcg
     1321 aaaaacgccc gagcgtccac catgcaggcg ggatacggcc acgggacggc catggggcga
     1381 ggcggcatgg ggccacgata cggaggtgca gcttggctta ccacagggaa cgccccgcac
     1441 tcacctcttc gtgcccatcg tttccgccgc gacccggccc gaagcgacac catggacgtc
     1501 gccgacatac cgagacaagg catcgaggcc tggcccccac ggcaagaccc gaggtctcat
     1561 caggggcgtt tgcccccggt ctgcaggcga tggagcctgc cgccgggctg ggcatgtttg
     1621 cctctctcgt gtcctcggac ttcgggaccc atctgaagcc ttgcggtgca ctgaacgacg
     1681 ggcaccaggc agggttcggg cgcccatgaa cgggaccgag gccgtcacac cgggttttcg
     1741 gccatgagcg gatgggcggc ccgggggacc gagagaaaca gtgggcacgg actgcgggca
     1801 ggagacgggc cgcatcaccg aacaaggagg cgacgggccc ggccgtgcac cgtggagaag
     1861 ggagggggac atggagagga gagacttcct gaaagcgctg gccggggtgt ccgttgcggc
     1921 cgcctgcccg ctcgggtcga gggcgagagc tgcacgttgc gcccccgaca ggcaagccgc
     1981 cctcaagaaa ggcgccgatc agaaggtata ccttgccggg gtgaaggccg gggcgtcgga
     2041 tgaggagctg aaggtcgcgg tgcgccaggc ggcccttgcg gccaccgact tttcctggct
     2101 gtccaggggc gacaccgtgt tcgtcaagcc ggcggtcaac tcgtcgaacc cctacccggc
     2161 caccacctgc ccggcggccc tggcggccat ggtcgaactc ctcaaggaca agggcgcagg
     2221 cagggtgatc tgcggcgaca tgtccggggt cgaacacgtg aggttcaccc gggaagggat
     2281 atcaggcagc tcgcgcaggc tcatggagtc gtgcggcata gccggtgccg ccctcgccgc
     2341 gggggcggag ctgcatttct tcgaggagca ggggtgggac gccttccacg aggaggcccc
     2401 ggccgagggt tcacactgga agcacggcct gatgatgccc gacatcctca gggaagtcca
     2461 gcacatcgta ctcatgccgc gctgcggcag ccacgtgctc gcagggagca ccctgggcat
     2521 gaaggccgcg gtcgggtact ggcggcacga tacgcgcctc gagtaccacc gcgacgcggc
     2581 gacgttccac cagaagaccg cggaagggaa caccgtggaa agcctgagga cgaagcagag
     2641 gctcgtggtc acctgcggcc acaaggtgct cgccacgttc ggccctgacg aggggtatgt
     2701 catggagccg caaaacggcc tcgtgatcgc ctccgagtcc attgtcgcgc acgacatggt
     2761 gtcgctcgcc tggctcctcg agaacaggcg cctcatgccg gattccgaaa agggatcctt
     2821 catggacacc agtcccttcg tcgcctggat aggcaaccgc atggtcgtca cctggctggg
     2881 cgggctcggc cagggcatcc tctccgacgg gttcgccaag cagcccgcag actccgtctg
     2941 ggacgacccc gtcctcgcga gggcctacga gctcttcggg ggcgttcccg gcgtcgtgct
     3001 cgagacggcc aacgccgctg tcgatgacgg cctgaaaaag cggctcgagg agatgaccgc
     3061 cctgccctcc tgacgtgcac agtcttcagg ccgggctccc agggcgcagg ccatgcagga
     3121 cggccgcctt gcctggggcg gcgcaggcat cgggcggccg cttgcgtggg gaacccctcg
     3181 gccgtgttga cacgcagccg ccgcaggggt atcagggtga aaaacgcgga ggtcagccag
     3241 ccatgatcgt ttgcatgaga aaccgaaccc ttgccgccct cgtctgtctg cacatggtct
     3301 tcttggccac ctcccccgcc ttcgcctcgc tcgtgccctc cgtggactcc cagggcaata
     3361 cgcaccgtag catcgaagac atgaagacca tcgagaaggc cctggagatg aagatcgtcc
     3421 aggaaaagct cagggcgtac ggcctggacg cccaggaagt gaaggccaag ctctcgtcca
     3481 tgagccccac ccagatccac accctcgcgc gcgcctgcga cgacgtgctc gcagggggcg
     3541 acagcgggct cggggtggtg atcgccctct tggtcatcgt gatcctcgtc atcatcatcc
     3601 tgaaactcct caacaaggag atcatcatca ggatgagcga ccgtgacggc cctgctgtcc
     3661 atactggcca tcctgtttgc tgaggcgccg caggccttcg tcgacggggt gccgttcatc
     3721 cggcaggatc cccgcctgtg cgggcccgcg gcgctcgcct cggtcctcgc ctattacggc
     3781 cttgcggtcg accagaaggc catcgcggac ttcgcctaca cggaaaagct cgggggcgcg
     3841 ctcatgaccg acctcgagaa ctacgcgcgc agcctcgggt gccggacgca cctcgcgcag
     3901 ggcacctcgg aagacctcat cgacgccctg gggaaaggca ggccggtcgt cg
//
LOCUS       DAOSCR010000210         3929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14563_length_3929_cov_3.886939, whole genome shotgun sequence.
ACCESSION   DAOSCR010000210 DAOSCR010000000
VERSION     DAOSCR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14563_length_3929_cov_3.886939"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLT69_06325"
     CDS             <1..820
                     /locus_tag="PLT69_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOJ14088.1"
                     /translation="LRNYICVGGDPEHWAFLDNFCWCDPVASDKNPDGEYKLAQLVRA
                     NKALYDYALAYRCPMISGKDSMKNDYIGAGIKISIPPTILVSVIGKIQDARRAVTMDV
                     KRSGSVLFVLGETRRELGASEYLAMHGLIGKRVPRVDAKSALRRYRLLHEAISGGLVL
                     AAHDCSDGGLGVCLAEMAFAGGYGMEVDLSLLKAEKGMNDTELLYSETPSRIVVEVDP
                     SKERAFRELFGRDAVALGGTTNTQRFVVRSRYGKTIADVPLDELLAAWKATLDF"
     gene            822..1634
                     /locus_tag="PLT69_06330"
     CDS             822..1634
                     /locus_tag="PLT69_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOJ14089.1"
                     /translation="MKRIRAIVITGYGTNCEVETAHACRLAGFSADIVHMSEILSGSK
                     SIKDYHFLNLPGGFLDGDDLGSAQVEGLRLRYAKIKTSGRSLYEEIVEFIDRGMLILG
                     VCNGFQALVKSGILPGTPRGTRKASLTFNDSARFEDRWVRLKVNAQSPCVFTRGVDSL
                     ELPVRHGEGKFVVDSEQTLSELRDKNMVVMRYADRSLRPTMEYPANPNGSVDAVAGIC
                     DPTGRIFGLMPHPEAHIHPTNHPAWTRRTAQPHSGDGLVLFENAMTFLKGVV"
     gene            1841..2653
                     /locus_tag="PLT69_06335"
     CDS             1841..2653
                     /locus_tag="PLT69_06335"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOJ14090.1"
                     /translation="MKFEPPAQAALEDVFSNPNTSNDADLLCRLMPAYLDELDAMMKK
                     NKYDLQAVFKASGAHFGYAFSCLEDTDKKAAMETYLRGRDLALAELRRYSFFDQSFDD
                     SLSQFKKALAYNFDKRNIQPLYWAAMNWFFLTTLDLNAQGSKEALQKAAAMLEFVAGI
                     DGGYGNGTTYAVLGSIHAILDEGEGGDVELAKQEFENAALYSGNSLLAINVLKARWLA
                     TRLKDRAMFESLLREVLSTPTDQFPDKTFVNVVARRKAQLLLENRRLFFPEG"
     gene            complement(2723..>3929)
                     /locus_tag="PLT69_06340"
     CDS             complement(2723..>3929)
                     /locus_tag="PLT69_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOJ14091.1"
                     /translation="QAQAILDMRLQRLTGLEREKLASEHASLTREIERLNRILSDKAV
                     LMDVIKQEISDIAEAFKDDRRSMIVQGGIGEFNVEDLIPDDNMVVTITRKGYMKRTEI
                     DKYRTQKRGGVGVRGASMSDDDFIEHIFVASNHTSILYFTTKGRVYHTKVYEIPERDR
                     ALKGVPIVNIRPIHKDERICAILAASDLEAEAEVAMVTAKGYIKRTELKNFRNILRTG
                     IIACSLEQGDEVISARLTTGNTKIIVASRQGKAIMFPAHQVRVMGRQAAGVRAIRLDE
                     NDTVVGMDVVTDDDAMVINLTENGFGKKTPVSKYPVQNRAGKGVYTVNITRKTGDLIG
                     LRVVRDSDELMIISSSGQIIRLGVNQVRTTKTRSAQGVRVIRLREDEKIVDFTRYVGE
                     DEDEDESPR"
BASE COUNT          756 a         1200 c         1202 g          771 t
ORIGIN      
        1 gctcagaaac tacatctgcg tgggcgggga ccctgaacac tgggcctttc tcgacaactt
       61 ctgctggtgc gacccggtgg caagcgacaa aaacccggac ggggagtaca agctcgcaca
      121 gctcgtcagg gcgaacaagg ccctgtacga ctatgccctg gcctacaggt gccccatgat
      181 atcgggcaag gacagcatga agaacgacta catcggcgcc ggcataaaga tctcgatccc
      241 gcccaccatc ctcgtatcgg tcatcgggaa gatacaggac gccaggcgcg ccgtaaccat
      301 ggatgtcaag cgttcaggca gcgtcctgtt cgtgctgggg gaaacgagaa gggagctcgg
      361 ggcgagcgaa tacctggcga tgcacgggtt gatcggcaag agggtgccga gggtggacgc
      421 gaaaagtgcg ctgaggcgct acaggctgct ccacgaggcc atctccggcg gactcgtcct
      481 ggcggcgcat gactgctccg acgggggtct cggcgtctgt ctcgccgaga tggcctttgc
      541 gggcgggtac ggcatggagg tggacctgtc cctcctcaag gctgaaaagg gcatgaacga
      601 taccgagctc ctctactcgg agaccccgtc gaggatcgtc gtggaggtcg atccctcgaa
      661 ggaacgggcg ttcagggagc tgttcggtcg ggacgccgtc gctctcggcg gcaccacgaa
      721 cacccaaagg ttcgtcgttc gatccagata tggaaagacc atcgcggacg tccccctcga
      781 tgaactcctg gccgcatgga aggccacgct agacttctga catgaaacgc atcagggcca
      841 tcgtcattac cgggtacggc accaactgcg aggtggagac cgcccatgcc tgcaggctcg
      901 cagggttctc ggcggacatc gtccacatga gcgagatcct gtctgggtcg aagagcataa
      961 aggactacca tttcctgaac ctgccagggg gcttcctcga cggcgacgat ctcgggtccg
     1021 cccaggtgga aggcctcagg ctcaggtacg ccaagatcaa gacctcgggg agatccctgt
     1081 acgaggagat cgtggagttc attgacaggg gcatgctcat cctcggggtc tgcaacggtt
     1141 ttcaagccct cgtgaaatcg gggatcctgc ccgggacgcc ccgggggaca cgaaaggcct
     1201 ccctcacctt caacgattcg gccaggttcg aggaccggtg ggtcaggctg aaggtgaacg
     1261 ctcaaagccc gtgtgtgttc acgagggggg tggacagcct cgaactgccc gtacgtcacg
     1321 gggaaggcaa gttcgtggtg gacagcgaac agaccctgtc ggaactcagg gacaagaaca
     1381 tggtggtgat gaggtacgcc gaccgatcgc tgcgcccgac catggagtac cccgccaacc
     1441 cgaacggatc tgtggacgcg gttgcgggca tttgcgaccc gacgggcagg atcttcggtc
     1501 tcatgccgca ccccgaggcg cacatccacc ccacgaatca ccctgcatgg acgcgccgca
     1561 ccgcccagcc gcactcgggc gacggcctgg tccttttcga gaacgccatg acgttcctga
     1621 agggcgtcgt ctgatctcga cacgcaggaa gccgaacgtt cacgacgaca gaaacagata
     1681 tcctctcgag atcctgtttg tgctatgatt cgttccatgt attgggggag ccgccacgct
     1741 cccatcccat accaaggagg ttgccatgaa ggtccctgca ggtaaggcga tggccatcgt
     1801 tgcccttgtc gcgctctgtt gcttgtctgc atgcaagcac atgaaattcg agccccccgc
     1861 ccaggctgcg ctggaggacg tcttctcgaa cccgaacacc tccaacgacg ccgatctgct
     1921 gtgcaggctc atgccggcat atctcgacga gctggacgcg atgatgaaaa agaacaagta
     1981 cgatctccag gctgtcttca aggcctcggg cgcacacttc gggtacgcct tctcgtgcct
     2041 cgaggatacg gacaagaagg cggcgatgga gacatacctc agggggaggg acctcgccct
     2101 cgccgagctc aggcgttaca gcttctttga ccagtccttc gacgacagtc tctcccagtt
     2161 caagaaggcc ctggcgtaca acttcgacaa gaggaacata cagcccctgt actgggccgc
     2221 catgaactgg ttcttcctca cgacgctcga cctgaacgcc cagggttcca aggaggccct
     2281 ccagaaggcg gcagccatgc tggagttcgt ggcgggaatc gacggcgggt acgggaacgg
     2341 caccacctac gcagtgctcg gttcgattca cgccatcctc gacgaaggcg aggggggcga
     2401 cgtggaactg gcaaagcagg agttcgagaa cgcagccctg tattccggga acagcctgct
     2461 cgccatcaac gtgctcaagg cccgctggct ggcgacaagg ctcaaggacc gggccatgtt
     2521 cgagagcctc ttgagagagg tcttgtccac gcccacggat caattcccgg acaagacctt
     2581 cgtgaacgtg gtggcgagga ggaaggcgca gctgctcctg gagaacagaa ggcttttttt
     2641 cccggaggga tgaccatcag ggtcgtctcc gtgccgtgag aacccgatcg cgtccggcgc
     2701 cgtcaaggcg ccggacggca tgtcagcggg gcgactcgtc ctcgtcttcg tcttctccca
     2761 cgtacctggt gaagtccacg atcttctcgt cctcgcgcag tcggatcacc ctgacgccct
     2821 gggcggaacg cgtcttggtc gtacggactt gattgacccc gagccgtatg atctgtccgg
     2881 agctcgatat gatcatgagc tcgtcggaat ccctcacgac cctgagcccg atgaggtcgc
     2941 cggtcttccg ggtgatgttc acggtgtaaa cgccttttcc cgcgcggttc tgaaccgggt
     3001 acttgctcac cggcgtcttc ttgccgaatc cgttctcggt gaggttgatg accatggcgt
     3061 catcgtccgt gacaacatcc atgcccacca ccgtatcgtt ctcgtcaagc cgtatggcgc
     3121 gcacacccgc cgcctggcgg cccataacgc ggacctggtg tgcggggaac atgatggcct
     3181 ttccctgcct ggacgcaacg atgatcttcg tgtttccggt cgtgaggcgc gccgatatca
     3241 cctcgtcccc ctgttccagg gaacatgcga tgatgccggt tctcaggatg ttcctgaagt
     3301 ttttcagctc ggtcctcttg atgtacccct tggccgtcac catcgccacc tcggcctcgg
     3361 cctcgaggtc tgatgcggcg agaatggcgc agatacgctc gtccttgtgg atcgggcgga
     3421 tgttcactat gggcacgccc ttgagcgcac ggtcacgctc ggggatctcg tacaccttcg
     3481 tgtggtacac ccgccccttg gtggtgaagt agaggatgga ggtatggttg ctcgccacga
     3541 agatatgctc tatgaagtcg tcgtcggaca tggaggcgcc cctgacgccc acgccgccgc
     3601 gtttctgcgt gcggtatttg tcgatctccg tgcgcttcat gtaacccttc cgggttatgg
     3661 tgacgaccat attgtcgtct gggatgaggt cctccacgtt gaactccccg ataccgccct
     3721 ggacgatcat gctgcgcctg tcgtccttga aggcctcggc gatgtcggaa atctcctgtt
     3781 taatgacatc catgagcacc gccttgtccg acaggatcct gttgagcctc tcgatctctc
     3841 tcgtgaggga tgcgtgctcc gatgcgagct tttctctctc gagcccggtc agcctctgga
     3901 ggcgcatgtc aaggatggcc tgggcctgg
//
LOCUS       DAOSCR010000211         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14664_length_3908_cov_2.977939, whole genome shotgun sequence.
ACCESSION   DAOSCR010000211 DAOSCR010000000
VERSION     DAOSCR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14664_length_3908_cov_2.977939"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..781
                     /locus_tag="PLT69_06345"
     CDS             185..781
                     /locus_tag="PLT69_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ14092.1"
                     /translation="MTISSRETRRREILEAAFEVLLELGPNKATLDDIAKRAGLAKTS
                     LYYYFDDKKEIVREIISHEIERLVSEMRAAVMGQETSFDKMLALVKTRYAFITSRARR
                     ASREIQEEFRTLAGVFEQERERYLSSHKELVEQILREGMRRGEIRPIRDPELISLIMI
                     SSMFGCDQTFAFYGQEERVMEGMRDMLGIFFRGLRPES"
     gene            859..1953
                     /locus_tag="PLT69_06350"
     CDS             859..1953
                     /locus_tag="PLT69_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ14093.1"
                     /translation="MQSVCGLRRVALVILLVCALLGASSEGCTKKEETAKQDKVVNVK
                     VQKVGKQTLRPMIEAVGSLTPVEETVVAAEVDGVVKEYLVDEGDRVARGQVLVRIKDT
                     DYLLAVDAARAALKLAEANLENALAQYGRMKALFDQQVVSKQEYDNMATRLDVARQER
                     ERAKAASDLAVQRLSKTTVTSPISGEVMHKAAAEGDFVGVGRPLVRVVDASSVKLTFS
                     IPEKEVTRVRKGQDVAFHVEAYPARTFSARVSSILPALDDRTRMLEVQALAPNASGEL
                     RPGMFARVSLFVGDPKPTVVIPVTSVLYEGTRTRVFVAEGGKALEREVTLGAKYGEFV
                     EVLSGVEEGETIIVVGQTAASDGVKVHVVE"
     gene            2016..>3908
                     /locus_tag="PLT69_06355"
     CDS             2016..>3908
                     /locus_tag="PLT69_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ14094.1"
                     /translation="MWISDTSIKRPVFATMVIMALVILGIVSYPSIGVDLFPKVDFPI
                     VSVTTVLKGASPEVVDIDVTDKIEEVINTISGVKNITSTSSEGVSSIIVEFELERDID
                     LAVQDVREQIAAVRRSLPEDIEEPVIRKVNPESTPVLWLNLTGKRSIAELSTYANEIL
                     KEQLQRIEGVGSLWLGGMRVRQVRVWLDLEKMKAYGVAPSDVAAVIGRENMELPGGRV
                     ESLTKEYAVKVKGNIMEVAAFGDLVVTRSEGRPVRIRDIARVEDGLEEERTYTRFNGM
                     PCVGIGVRKQTGTNTVEVIDRVKKEIDRINRDLPGGMRINIAFDQSIFIKDSINQVQH
                     HLIIGSILAVVAVFVFLGNVRTTLISAIALPVSIISTFTLMRAFDFTFNNLSMLALTL
                     SVGILIDDAIIVIENIYRHIEEGMAPREAASFATSEIGMAVTATTLAIAVIFLPVAFM
                     KGIIGRFFLQFALTVVFAVGVSLLVSLTLTPMLSSIFLSPIAKHRREGSLLSRLSGAF
                     EKAYKRVEAFYRPILAYSIDHRGAVLLGTLVLFVVSMYLVRFIGKEFVPPEDQSQFIV
                     RLETPVDYSFPKARELFLKAEQMVRARPEVNGAFFIQGIGQGGAGGQINMAAIFVRLV
                     PRGDRVK"
BASE COUNT          790 a         1108 c         1294 g          716 t
ORIGIN      
        1 gaagcgcttt ttctctttgc ggtccgttac cttgcgactg gtgacaaggt gcagaacata
       61 tcgaagcgac cgtgagttca tggtgtggtg tcgtaaagga ctttcgaaaa ggggaggtcc
      121 tgcgttgaca ggtgttttcg tctcgtgtag aactataaaa cattctattc gaaagcttga
      181 atgcatgacg atttcttccc gcgagaccag gaggagggag atcctcgagg cggcgttcga
      241 ggtgctcctc gagctcggac cgaacaaggc gacgctcgac gacatagcga agagggccgg
      301 gctcgccaag acctcgttgt actactactt cgacgacaag aaggagattg tgcgcgagat
      361 catcagtcac gagatcgaga ggctcgtctc ggagatgagg gcggcggtca tggggcagga
      421 gacgtccttc gacaagatgc tcgccctcgt caagacgcgc tatgcgttca tcacctcgag
      481 ggcgaggcgc gcttcgcggg agatacagga ggagttcagg acgctcgccg gggttttcga
      541 gcaggagcgc gagaggtacc tctcatccca caaggaactg gtggagcaga tcttgaggga
      601 agggatgaga cgcggcgaga tcaggcctat cagggaccct gagctcataa gcctcatcat
      661 gatctcgtct atgttcgggt gtgaccagac gttcgccttc tacggccagg aagaacgtgt
      721 catggagggg atgagggaca tgctcgggat ctttttcagg ggtctcaggc ctgagagttg
      781 agagatcagt gccttggttg gacaacagat acagggggtg aaatgagctt gttcccagat
      841 cgcgaatttg aagggttcat gcagagcgtg tgcggcctcc ggcgtgtcgc cctcgtgatc
      901 ttgctcgtgt gcgcactgct cggagcttcc tccgagggct gcacgaagaa ggaggagacg
      961 gcgaagcagg acaaggtcgt caacgtcaag gtccagaagg tgggcaagca gaccttgagg
     1021 cccatgatag aggcggtggg atcgctcacg ccggtagagg aaacggtggt ggccgcagag
     1081 gtggatggcg tggtcaagga atacctcgtg gacgagggag acagggtcgc caggggacag
     1141 gtcttggtca ggatcaagga caccgactac ctgctcgccg tcgatgccgc cagggccgcg
     1201 ttgaagctcg cagaggcgaa cctcgagaac gctctcgccc agtacgggag aatgaaggcg
     1261 ctcttcgacc agcaggtcgt ctccaagcag gaatacgaca acatggcgac gaggctcgac
     1321 gtcgccaggc aggaacggga aagggcgaag gccgctagcg accttgccgt gcaaaggctt
     1381 tcgaagacca cggtgacctc gccgatatcc ggggaggtca tgcacaaggc cgcggccgag
     1441 ggcgatttcg tgggcgtcgg caggcctctc gtgagggtcg tggatgcatc cagcgtgaag
     1501 ctcaccttct cgatacccga gaaggaggtg accagggtca gaaagggcca ggatgtggcg
     1561 ttccatgtgg aggcctaccc ggcgaggacg ttcagcgcac gggtgagctc catcctgccc
     1621 gccctcgacg accgcaccag gatgctcgag gtgcaggccc tcgcgccgaa tgcctcgggc
     1681 gagctcaggc cggggatgtt cgcgagggta tcgttgttcg tgggagaccc gaagcccacg
     1741 gtggtgatcc cggtcacgtc cgtgttgtac gagggtacca ggacgagggt gttcgttgcc
     1801 gagggaggga aggcccttga gcgtgaggtc accctgggcg cgaagtacgg cgagttcgtg
     1861 gaggtgctct cgggcgtcga ggagggagag accatcatcg tggtcggcca gaccgcggcg
     1921 agcgacgggg tcaaggtaca tgtcgtggag tgagacagcg tggtgttcta cggtttcggg
     1981 aagggataga accgcccccc acaggagtgt gaaccgtgtg gatctctgat acctccatca
     2041 agagaccggt cttcgccacc atggtcatca tggccctggt gatactgggc atcgtgagct
     2101 acccgtccat aggggtcgac ctcttcccga aggtggattt ccccatcgtg agcgtcacca
     2161 ccgtgctcaa gggcgcgagc ccggaggtcg tggacatcga cgtcaccgac aagatcgagg
     2221 aggtgatcaa taccataagc ggcgtcaaga acatcacctc gacgagctca gagggggtct
     2281 ccagcatcat cgtcgagttc gagctcgagc gcgacatcga tctcgccgtt caggacgtgc
     2341 gcgagcagat cgcggccgtg aggaggtcgc tgcccgagga catcgaagag ccggtcatca
     2401 ggaaggtcaa tcccgagagc acgccggtgc tctggctcaa cctgaccggc aagaggtcga
     2461 tagcggaact ctccacgtac gccaacgaga tcctcaagga acagctccag aggatcgagg
     2521 gcgtggggtc gctgtggctc ggcggcatga gggtccgaca ggtgcgcgtc tggctcgacc
     2581 tcgaaaagat gaaggcctac ggggtcgcgc cgtccgacgt tgccgcggtc atcggcaggg
     2641 agaacatgga gctgcccggc ggccgcgtcg agagcctcac caaggagtac gccgtcaagg
     2701 tcaaggggaa catcatggag gtcgccgcct tcggcgacct cgtggtgacc aggagcgaag
     2761 gccgccccgt gcgcatacgg gacatcgcca gggtggagga cggcctcgag gaggaacgga
     2821 cctatacccg gttcaacggc atgccctgcg tcggcatagg cgtcaggaag cagaccggca
     2881 cgaacacggt ggaggtcatc gaccgggtca agaaggagat cgaccgcatc aacagggatc
     2941 ttccgggcgg gatgcgcatc aacatcgcct tcgaccaatc catcttcatc aaggactcga
     3001 tcaaccaggt ccagcaccac ctcatcatag ggtcgatcct ggcggtcgtc gccgtcttcg
     3061 tgtttctcgg gaacgtgcgg accaccctca tcagcgcgat cgccctgccc gtctccatca
     3121 tctccacctt caccctcatg cgcgccttcg acttcacctt caacaacctc tccatgctgg
     3181 ccttgaccct gtccgtgggg atcctcatag atgacgccat catcgtgatc gagaacatct
     3241 accgccacat cgaggaaggc atggcgccca gggaggcggc ctcgttcgcc acctccgaga
     3301 taggcatggc ggtgaccgcc accacgctcg cgatcgcggt catcttcctg cccgtggcct
     3361 tcatgaaggg catcatcggc aggttcttcc tccagttcgc cctcacggtg gtcttcgcgg
     3421 taggggtatc gctgctcgtg tcgctgacgc tcacgcccat gctctcctcc atcttcctct
     3481 cccctatcgc gaaacatcgt cgagagggat ccctgctatc acggctctca ggcgccttcg
     3541 agaaggccta caagcgtgtc gaggcattct acaggccgat cctcgcctat tccatcgatc
     3601 accgtggcgc cgtgctcctg ggcacgctgg tgttgttcgt ggtcagcatg tacctggtgc
     3661 ggttcatcgg aaaggaattc gtgcccccgg aggaccagtc gcagttcatc gtgaggctgg
     3721 agacgcccgt cgactattcc ttccccaagg cccgggagct cttcctgaag gccgagcaga
     3781 tggtgagggc caggcccgag gtgaatggcg cgttcttcat ccaggggatc ggccagggcg
     3841 gggccggcgg ccagatcaac atggccgcga tcttcgtgag gctcgtgccc aggggggata
     3901 gggtgaag
//
LOCUS       DAOSCR010000212         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14687_length_3904_cov_3.037412, whole genome shotgun sequence.
ACCESSION   DAOSCR010000212 DAOSCR010000000
VERSION     DAOSCR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14687_length_3904_cov_3.037412"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..1128)
                     /locus_tag="PLT69_06360"
     CDS             complement(529..1128)
                     /locus_tag="PLT69_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOJ14095.1"
                     /translation="MRLVQPLLKGGMGLDEALAKRRTVRSFSPAPLTREQLSQLLWAG
                     QGATDEAGFRRTSPSAGALYPIDLLAVIGSGSVEQMEAGVYLYESRTHSTTLLVEGDH
                     RDELARASLSQFWMAKAPVSLVVAAEYGRVASKYGQRGERYALLEAGHVAQNIMLEAV
                     SLGLASAPVGAFVDTEVRNLLCLEARISPLLILPVGYPK"
     gene            1512..2711
                     /locus_tag="PLT69_06365"
     CDS             1512..2711
                     /locus_tag="PLT69_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ14096.1"
                     /translation="MAQNTIPLEEIRLLASGLFDETVRVRRDIHAHPELGFSEERTAG
                     IAASCLSELGIEVFTGVAKTGVVGVLDGKTPGRTLLLRADMDALPLQELADVAFRSRV
                     PGVMHACGHDGHTAVLISAARLLARLRDRLKGRVVFVFQPAEENLGGAKAMIDEGAFS
                     GLGIEAALGLHLITLLPCGTVGLRPGPFMASVDLFRALILGEGGHGAMPGGSVDAIEI
                     AAKVVSGLKAHLAGAITPGGRPLVNIGTIKGGTAPNIVADTVELEGTVRCMDDDARPA
                     LRQAMEAFLESVAKDAGGSYELAYTDGYPILVNDHHMTDIVRRTAQDLLGETRVFELE
                     PTMASEDMAFFLRMVPGCFFFVGAGFGDPAMDYPHHSPRFVLNEDSLLVALQVLCTAA
                     VRFLEGA"
     gene            3042..>3904
                     /locus_tag="PLT69_06370"
     CDS             3042..>3904
                     /locus_tag="PLT69_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOJ14097.1"
                     /translation="MRPELSDTAEYILRLRYLKKDAEGNVAETPEELFRRVADHVGRA
                     ETVFGADAREAGKVADEFFETLTRLEFLPNSPCLMNAGRELGQLAACFVLPVNDSLES
                     IFEAVKDTALIHQSGGGTGFSFSRLRPKNDTVLSTSGVACGPVSFMEVFDKATDVIKQ
                     GGARRGANMGVLRVDHPDIEEFVTAKRVPSRLSCFNLSVGVTDSFMEAVLEDGLVGLV
                     NPRNKAVTKTIRARALFDLIVQCAWATGEPGILFLDEINRANPTPDLGTIEATNPCGE
                     QPLLPYESCTLG"
BASE COUNT          693 a         1269 c         1312 g          630 t
ORIGIN      
        1 ctgcatgatg cctacatcgc agatgccaat gtacagaaaa tgggggactt gactgggcat
       61 gtggggtttc agcgggaagc ggccgctgta aagaatcgag aagaagacca cagggctgag
      121 aaacatcgca acgcctcaaa cgaccatttt ccagcatcgg aggggctttc ttcgtgatac
      181 ccacaggccg gggcctctct tcgcataatc gagcaaaggc tctcccgggg gagacacagc
      241 cctgcatgcc tcggcctgat acccgccgag cgcggctgta cccggtatgg ctcggcagta
      301 tctcctctgt gcgtgatcta attgggacgg ctcggctgca ttcccgccgt tcgtgatcca
      361 accggggtgg tccggtctgg ctcggcgggt gccacgcagg gcgcgaacac acctcgatta
      421 accaaggaga agccccgcta agtgcgacct tgtccgggat gaggcagccg aagccccacc
      481 gggcgggatc tcacccatgg taaccggtgg tacgcccctg gagcgccctc acttggggta
      541 accgacgggc aggatcaaca gcggggagat ccgggcctcg aggcagagga ggtttcggac
      601 ctcggtgtcg acgaacgctc ccaccggggc cgatgccagc ccgagcgaga cggcctcgag
      661 catgatgttc tgcgccacgt ggcctgcctc caggagggcg tacctctcgc ccctctgccc
      721 gtacttggac gcgacccggc cgtactcggc cgccaccacc aggctcacgg gcgccttggc
      781 catccagaat tgggagagag aggcgcgcgc gagctcgtcg cggtggtctc cctcgacaag
      841 gagcgtggtc gagtgcgtgc ggctctcgta gagatacacg cccgcctcca tctgctcgac
      901 cgaccccgag ccgatgaccg ccaagagatc gatggggtac agcgcgccgg cggaaggaga
      961 ggtcctcctg aaaccggcct cgtcggtggc cccctgccct gcccacagga gctgggagag
     1021 ctgttccctg gtcaggggtg cgggggaaaa cgaacgtacg gtcctcctct ttgccagggc
     1081 ctcgtccagg cccatgccgc ccttgagcag aggttgcacg agtctcattc cagcctccct
     1141 gatcggactg tgaaacgagc ggtcagagat gaacgatccg agatcaagga taggacgtgt
     1201 cgcgaccata tgcaagggcc ttccctgcgg gcacggtcgg aaggtcatgc ccgcccgtct
     1261 cttgaaccgg cttcacggcc attccctgtg ggcacggtcc gaggcttcct ggataccccg
     1321 atctgatgga tcaggggcca ttccctgtag gcacgggctg agatcctgat gcggcccccg
     1381 caggaccgcc ccatcaggcc attccctgtt ggcacggtat gatgacatcc cgaaagcccg
     1441 gatcgctgcc gatgccgcac tcgagccttg cctggggctc tcctcgtcgt ataatgtccc
     1501 ggggataccg catggcacag aacacgatcc ccctggagga gatccgcctg ctggcctcag
     1561 gtctcttcga tgagacggtc agggtccggc gggacatcca cgcgcacccg gaactgggct
     1621 tctcggagga aaggaccgcg ggcatcgcgg cgtcgtgcct gtcggagctg gggatcgagg
     1681 tcttcacggg cgtcgcgaag acgggcgtgg tcggtgtgct cgacgggaaa acgccgggca
     1741 ggacgttgct gctccgcgcc gacatggacg ccctgccgct acaggaactc gccgacgtgg
     1801 cgtttcgttc ccgggttccc ggcgtgatgc acgcatgcgg ccacgacggc cacacggccg
     1861 tgctcatctc cgccgcccgt ctcctcgcga ggcttcgcga caggctgaaa ggcagggtcg
     1921 tcttcgtgtt ccagcccgcc gaggaaaacc tcggcggcgc caaggccatg atcgacgagg
     1981 gcgcctttag cggcctcggg atagaggccg ccctcggcct ccacctcatc accctcctgc
     2041 cgtgcggcac cgtggggttg aggccggggc cgttcatggc ctcggtggat ctcttcaggg
     2101 cgctcatcct cggcgaaggc gggcacgggg ccatgccggg cggctccgtg gacgccatcg
     2161 agatcgcggc caaggtcgtg agcggcctca aggcccacct cgcgggcgcg atcacgccgg
     2221 gcgggcgacc cctcgtcaac atcggcacca tcaagggagg caccgccccc aacatcgtcg
     2281 cggacacggt ggagctcgag ggcacggtga ggtgcatgga cgacgatgcg aggcccgccc
     2341 tcaggcaagc catggaagcc ttcctcgagt ccgtagccaa ggatgcgggc gggtcttacg
     2401 agctcgcgta cacggacggc taccccatac tcgtcaacga tcaccacatg accgacatcg
     2461 tcaggagaac ggcgcaggac ctgcttggcg agacacgagt gttcgagctg gagcccacca
     2521 tggcgagcga ggacatggcg ttcttcctcc gcatggtgcc gggctgcttc ttcttcgtgg
     2581 gggcgggctt cggcgacccc gccatggact atccgcacca cagcccccgg ttcgtgctca
     2641 acgaggactc gctcctggtc gccctccagg tcctctgcac cgcggcggtg cgcttcctgg
     2701 agggcgcgtg agcggccctc ggtgcggcgg aatgcccttc tttgcggctc gacggctgta
     2761 cgccgcggaa aacggcgatc gcgacgagac gttgcctggt ggacgaaatg ccccgtcagc
     2821 cgtacccgga gaaatcccgg gtcggcaggg gccggcagga gactgtgcct tcgcgggctc
     2881 catccagggc gggcgagccc gaggtcatga gtgaagccca tgcggaaatg ccgggcggga
     2941 aaggcatcac gtgacgaaga gccgtgtccg ccgggacact ggcggggaaa cccgcctgaa
     3001 gcggccccga cggaaaaggc catgtgcagg gagcgactga catgcggccg gagttgagcg
     3061 atacggcgga gtacatcctg aggctacggt acctcaagaa ggatgccgag gggaacgtcg
     3121 cggagacccc tgaagagctg ttccggcgtg tggccgacca cgtgggtcgc gccgagacgg
     3181 tgttcggggc cgacgcacgc gaggcgggca aggtcgcgga cgagttcttc gagacgctca
     3241 caaggctcga gttcctgccc aactcgccgt gcctcatgaa cgcgggcagg gagctggggc
     3301 agctcgcggc ctgcttcgtc ctgccggtga acgactcgct cgagtccatc ttcgaggcgg
     3361 tcaaggacac ggccctcata caccagagcg gaggcggcac cgggttttcc ttcagcaggc
     3421 tcaggccgaa gaacgacacg gtcttgagca ccagcggcgt ggcctgcggc cctgtctcgt
     3481 tcatggaggt cttcgacaag gcgaccgacg tgatcaagca gggaggcgcg cgcaggggcg
     3541 cgaacatggg cgtgctcagg gtggatcacc cggacatcga ggagttcgtc acggccaagc
     3601 gcgtcccgtc cagactgagc tgcttcaacc tctcggtcgg cgtgaccgac tccttcatgg
     3661 aggcggtgct cgaagacggt ctcgtggggc tcgtcaaccc caggaacaag gcggtcacca
     3721 agacgattag ggcacgagcg ctcttcgatc tcatcgtcca gtgcgcgtgg gcgaccgggg
     3781 aacccggcat cctcttcctg gacgagatca acagggcgaa ccccaccccg gatctcggga
     3841 cgatcgaggc caccaacccc tgcggcgagc agcccctctt gccgtacgag tcgtgcacct
     3901 tggg
//
LOCUS       DAOSCR010000213         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14841_length_3874_cov_4.073579, whole genome shotgun sequence.
ACCESSION   DAOSCR010000213 DAOSCR010000000
VERSION     DAOSCR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14841_length_3874_cov_4.073579"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PLT69_06375"
     CDS             complement(<1..1431)
                     /locus_tag="PLT69_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ14098.1"
                     /translation="MDEHGVDRTARTESDGLTTHGGAAEDIHIKDYAIASSINGMAIG
                     DLDGVVTYVNKAALSIYGTDDPSDIIGKRATIFAKSEQEALGILETVLVKGEWSGEVS
                     GWRKNGEAVTVLLSASLVKDDAGRPVCIVCSFIDITERKRMEEELRVKESAIASSING
                     IVIGDPSGTITYVNKAALDMWGAKDPSEIMGRHVTVFAESEQEAREILSIVLEKGSWS
                     GEITGRTMSGAPITVHLTASLVKDEHGEPAHLMCSFIDVTEHKRIENELRIKECAIAS
                     SLNGIGIGDLEGNILYVNEAALKMWGADDPSEVVGRSALEFAESQEEAIRVFSEFMEK
                     GGWSGEISGRRKDGSLITVHLTASLVKNERGEPICTMDSFLDITERKRMEEELRIKDL
                     AIASSIDGIGITDLEGNVIYVNEAALRMWGAEDPSEVIGRSAFSFARSQEEAMEIYRS
                     VLENGSWSGEVSGYRKDGSPITVHLSV"
     gene            complement(1835..3385)
                     /locus_tag="PLT69_06380"
     CDS             complement(1835..3385)
                     /locus_tag="PLT69_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14099.1"
                     /translation="MRRSLYAAVLAATLAIALAPAGPAEADGPGDESLSSQAFSSPPS
                     AATAQAVKDPVEEGITHQELSTSPQSESLLGFIRSLLSPTRRQEARLENPAPGPGLES
                     KPSSSSMPRTAIEPAADRGPGDEIVHAELDTTPQTGGILGSASGQTTAVETLMTAGSD
                     VRVPSPMQATETAADKDEPAHLELADVPQKTGILGLYTTNVQAQPQETAGPAGPTGAD
                     GTAQRGVKLAQASGEEELTHQALATAVSEKDTFSFVSAGVLVPLVDSTFKKFVDYGGM
                     VTMGARKRMSDKLTLSATVGVGVLSGDWSIKGDRQSILVAAEEYYPGYVKEPGVVITP
                     EELPDENLGISYHAEAEAVVTSSESLERIDVHTDLYLFPVSLNAVYQVKQSPKFNAYV
                     TGGLGFCTAVRDCDSKAVKEKYFQGPEYRVAINRSQSVTGLLVNLGAGMSMPVYGRLT
                     FVAEATTTLYDLKAFDPVLEVSFTRPNPDWYPGSDLSQWSYEDPVRVGVYKEVYVTNI
                     QVGFVVPF"
BASE COUNT          592 a         1273 c         1228 g          781 t
ORIGIN      
        1 caccgacagg tgcacggtga tgggcgagcc gtccttgcgg tagcccgaga cctcccccga
       61 ccagctgccg ttttcgagca ccgaccggta gatctccatg gcctcctcct gggacctcgc
      121 aaaggagaag gccgaccggc cgatgacctc cgaggggtcc tcggcgcccc acatcctgag
      181 ggccgcctcg ttgacgtaga tgacgttgcc ctcgaggtcc gtgatcccga tgccgtcgat
      241 ggaggacgcg atggcgaggt ccttgatcct cagttcctct tccatgcgct tgcgttccgt
      301 gatgtccagg aacgagtcca tggtgcagat gggctcaccc ctctcgttct tcaccaggct
      361 cgcggtgagg tgcacggtga tgagggaccc gtccttcctc ctgccggaga tctcgcccga
      421 ccagccgccc ttctccatga actcggagaa gaccctgatg gcctcctcct gggactcggc
      481 gaactccagg gcggaccggc cgacgacctc cgaggggtcg tcggcgcccc acatcttgag
      541 cgccgcctcg ttgacataga ggatgttgcc ctcgaggtcg ccgatgccga tgccgttgag
      601 agaggaggcg atcgcgcact ccttgatcct cagctcgttc tcgatcctct tgtgctccgt
      661 cacatcgatg aaggagcaca tgaggtgggc cggctcccca tgctcgtctt tcaccaggct
      721 cgccgtgagg tgcaccgtga tgggcgctcc gctcatggtc ctcccggtta tctcgccgga
      781 ccagctgcct ttctcgagca cgatcgagag gatctctctt gcctcctgtt cggactcggc
      841 gaacacggtc acgtgcctgc ccatgatctc cgaggggtct ttggcgcccc acatgtcgag
      901 ggcggccttg ttcacgtagg tgatggtgcc ggaggggtcg cctatgacga tgccgttgat
      961 ggaggacgcg atcgccgatt ccttgaccct gagctcctcc tccatgcgct tgcgctccgt
     1021 gatgtcgatg aaggagcaca cgatgcagac aggcctcccc gcgtcgtcct tcacgaggct
     1081 cgcggagagc agcacggtca ccgcctcgcc gtttttcctc cagccggaga cctcgcccga
     1141 ccactcgccc ttgacaagca ccgtctcgag tatcccgagc gcctcctgct cggacttggc
     1201 gaatatggtc gcgcgcttgc cgatgatgtc ggaggggtcg tcggtaccgt atatggagag
     1261 cgcggccttg ttcacgtagg tgacgacgcc gtccaagtcg cctatggcca tcccgttgat
     1321 ggacgaggcg atggcatagt cctttatgtg gatgtcttcg gccgcaccac cgtgggtcgt
     1381 caggccgtcg gactctgtac gggcggttct gtccacgccg tgctcatcca tcgccgttcc
     1441 tccgctttgg gtcgtgtgtg cgcctgctgg cgggatgtcc catcgcaagt cggtgggtca
     1501 tgcgtgaggt gcttctacca tacgaggtcg cggctgggca acatggtgcc aaaggggctc
     1561 tcgtcgccgc gtccggccct cctggacgtg gggcctcatg gccccgggag ccgctgctct
     1621 tcttccacag ggcgcctcgg gcctcgacgc acagggcgtc agggtctctg cgcgtctctg
     1681 cgactgcctt cgacggtagg ccccggtcga gattcgctgg gtgcggcggc aggcctcgca
     1741 cccgcactgc atccataccc ttgagaacga gaccgaaagg catactcagg ccgtcgacgt
     1801 caggcccccg gccgtgcgaa aacccgcata tgcatcagaa cgggaccacg aagcccacct
     1861 ggatgttcgt cacgtagacc tctttgtaca cgcccaccct caccgggtcc tcgtagctcc
     1921 actgggagag gtcggagccg gggtaccagt ccggattggg cctggtgaac gagacctcca
     1981 gcaccgggtc gaaggccttc aggtcgtaca gggtggtggt cgcctccgcc acgaacgtga
     2041 gcctgccgta caccggcatg ctcatgcccg cgcccaggtt caccagcagc cccgtgacgg
     2101 attgcgagcg gttgatcgcc acgcggtact cggggccctg gaagtacttc tccttgacgg
     2161 ccttgctgtc gcagtccctc accgcggtgc agaagccgag gccgccggtt acgtacgcgt
     2221 tgaacttcgg gctctgcttg acctggtaga ctgcgttcag tgacacgggg aagaggtaga
     2281 ggtcggtgtg gacgtcgatg cgctcgagcg actcggagct cgttaccacg gcctctgcct
     2341 cggcgtggta gctgatgccg aggttctcgt ccggcagctc ctcgggggtg atgaccacgc
     2401 cgggctcctt cacgtagccc gggtaatact cctcggccgc cacgaggatg gactgcctgt
     2461 cacccttgat ggaccagtcc ccgctcaaca cgcccacccc gacggtggcg ctcagggtga
     2521 gcttgtcgct catgcgcttt ctcgcgccca tggtgaccat gccgccgtag tctacgaact
     2581 tcttgaaggt cgagtccaca agcggcacga ggacgcccgc ggagacgaag gagaacgtgt
     2641 ccttttctga cacggcggtc gccaaggcct ggtgggtcaa ttcctcttca ccgctcgcct
     2701 gggccagctt cacaccgcgt tgcgccgtgc cgtctgcgcc cgttgggccc gcgggaccgg
     2761 ccgtctcctg gggctgcgcc tgcacgttcg tggtgtacag gcccagtatg cccgtttttt
     2821 gggggacgtc tgcgagctcg aggtgcgcgg gttcgtcctt gtccgcggcg gtctccgtcg
     2881 cctgcatggg ggagggcacc ctgacgtcgg atcccgctgt catgagggtc tcgaccgctg
     2941 tggtctgccc ggatgccgaa ccgaggatcc cgcctgtttg cggggtcgtg tcgagttcgg
     3001 cgtgcacgat ctcgtcgcct gggcctcggt ctgcagccgg ctcgatggct gtcctcggca
     3061 tggaggacga agagggcttg gactcaaggc caggacctgg ggccgggttt tcgagacgcg
     3121 cctcctgccg ccttgtcggt gagagcaggg agcgtatgaa cccgagcagg ctttcgctct
     3181 ggggggatgt cgacagctcc tggtgcgtga tgccctcctc caccgggtct ttcacggcct
     3241 gggcggttgc ggcgctcggc ggcgacgaga aggcctgtga ggacagcgac tcgtccccgg
     3301 gcccgtccgc ctcggccggg cccgccggcg caagggcgat cgccaaggtt gcggcaagaa
     3361 cagctgcgta cagagagcgt ctcatgtctg atccctcccg gcggccttcg tccagaacgg
     3421 gccgccccat acaatgaaag acatcatcgg tctcgatcct ccaaggtctg cctgctcgac
     3481 ctgccttgtc ggtgcctgcg tcataataga acaaaaaccc cgaacccgta agggggcagt
     3541 tgggtgcttc ccaacccgct tcatgcatgc tccatctttc tccagcctta ctccaggggc
     3601 tcgacaggcc gttgcatgcc ggcatcgagc cgtgcgcatg ctcgtgcagc gcagggatca
     3661 cggccgtcgt cacgcacgaa tacccccttg tttttgtgca agaaacattc ccttgacacc
     3721 gctcgatggt acgtgagtga tctcgggaca ttgcacggca tctccatggt gcgggatccc
     3781 aggcgagacc gggtgtgtgc attttcaggg caacaagtga gcaacatttt tctcctgcag
     3841 agggcgtctt ggtgcctgga cgtgtcaccc ctga
//
LOCUS       DAOSCR010000214         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14855_length_3872_cov_3.051611, whole genome shotgun sequence.
ACCESSION   DAOSCR010000214 DAOSCR010000000
VERSION     DAOSCR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14855_length_3872_cov_3.051611"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLT69_06385"
     CDS             <1..247
                     /locus_tag="PLT69_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOJ14100.1"
                     /translation="TQMAGEKGYQLCKAYMKTWADLGIDHVQFNCVSDETLRAAQRDP
                     EKYPELIVRVAGYSAHFVDISRKTQDNIIQRTVQGLA"
     gene            332..700
                     /locus_tag="PLT69_06390"
     CDS             332..700
                     /locus_tag="PLT69_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14101.1"
                     /translation="MARMSRDEYAKASGVFGVKPGAGSAVEDLDKVPERPCGLCKHCL
                     EVAWSSDGRGSCNVLKEGSDITCDPPVFVLEGKNGYMMRLLTDASRCTYYERNEFIDK
                     DGFECSDPVFRRFIRQLRDK"
     assembly_gap    897..906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1114..1452
                     /locus_tag="PLT69_06395"
     CDS             1114..1452
                     /locus_tag="PLT69_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14102.1"
                     /translation="MTCHKCGYKDSYTAFAYLCKNGCPACGESDLRRCPKCGAQCVFS
                     RAEALEGEEREMEGLVGRLMEIHAGSTPEEILEAKRIMARLSDMNNRWNLSRLRELLI
                     LKQKEFTSQR"
     gene            1469..2365
                     /locus_tag="PLT69_06400"
     CDS             1469..2365
                     /locus_tag="PLT69_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ14103.1"
                     /translation="MQETKVVTQTRKDTRRLPGSDGLIPVPGREDHFFLKAEVIRLLE
                     RVELLSERHPVNVLVVGPQGCGKSTLVRQFAARFNRPLAVFQVGILSEAGQLFGEHRL
                     EEGRTYFNEFLFPKAVRTPRCVIHLEEINRPEHPKALNMLLSLLAEDRSVWIDEIGRV
                     DVAPGVVFFATMNEGEEFVGTEMLDAALRDRFYIVALDYLPPEVETQVLRLKANLGEA
                     DALTVVNVANRLRTNRNDPVTVSTRHTLMIAEMVGLGASAKDAFVSGLQVGRDTLETV
                     LLSVHLEGRLPGGEEKDEYHEY"
     gene            2346..>3872
                     /locus_tag="PLT69_06405"
     CDS             2346..>3872
                     /locus_tag="PLT69_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOJ14104.1"
                     /translation="MSTTSIDGTKEPQGHGPLSASWRRNLSSVEALEMANVLRALRKV
                     VGHLGTNAPEVEYAGFPSTGANSIFVDPARVMGTYPVPHETSDVLIGEVVHEAILGIE
                     WTDRVRSLLVKHMSGLGMIELVKFQRMVSTGEHIYADAVLDGTVLGAYASKARRHALR
                     NSWAVRSREKSGFSYDTLMLSWIQASFLDGGSHTVHKGLARCMDVLLDLTASLARIAG
                     SSRGQVFRCEERSSLYLKAWSALEPLVRDVPVIDRRLVWSPGDDPSPARKAPGHRLGT
                     QAHPACTEDLVRRVELELARSSSDLTPLVRAALGGDDGDVVPISRWDYRVSAHPVVDR
                     AMAARIAAVFRIYAERRPRLSRGLAAGRVDGRRLYRVPLSGRCFFEREAHKEMDWNIT
                     LLCDASGSMRGAKWKTVESTIATICKAIEAYRGMLNAYAYFENDGVCMLSSLLEGGRL
                     FAVPPGGRTASGQAIIAAGLLMPSSNRRRLLIHVTDGESNYGCSVQKGIDFCRAQGIH
                     L"
BASE COUNT          752 a         1131 c         1341 g          638 t
ORIGIN      
        1 gacacaaatg gcgggcgaga aagggtacca gctctgtaag gcgtacatga agacatgggc
       61 cgatctcggc atcgaccacg tgcagttcaa ctgcgtgtcg gacgagaccc tcagggccgc
      121 ccagcgcgac ccggagaaat acccggagct gatcgtcagg gtcgcgggtt acagtgcgca
      181 cttcgtcgat atcagccgca agacacagga caacatcatc cagaggacgg tccagggact
      241 ggcatgaacg atccgggggc cagagcgcct gtggggatga agacctgtcg ggtgaacgga
      301 caacgacggg gaaacaaaag gagatggtgt catggcgagg atgagcaggg atgagtatgc
      361 aaaggcatcg ggtgtgttcg gggtcaagcc gggggcgggt tcggcggtgg aggaccttga
      421 caaggtgccg gaacgcccat gcggcctgtg caagcattgt ctcgaggtgg catggtcgag
      481 cgacggccgg gggagttgca atgtcctgaa ggaggggtcg gacatcacct gcgacccccc
      541 ggtcttcgtc ctcgagggga agaacgggta catgatgagg ctgctgactg acgcgtccag
      601 gtgcacgtac tacgagagga acgagttcat cgacaaggac ggcttcgagt gcagcgaccc
      661 tgtgttcagg cgtttcatac gccagctgag agacaagtga cgacggtcgt ttcgagggga
      721 gtgcgaggga tggaccaagg aaactcaaag gagagaacac atgaagtgca ctgaatgcgg
      781 atacgacgca cccgtggcga aatttcggta tttgtacaac ccccgcatcg atgcgcccat
      841 aaccctgagg cagtgcacgc agtgccaggc atggctcgtg gtggacgagc tcaaggnnnn
      901 nnnnnnttgc caagaagagc atcggccccg gtgaagatcc ctggggcaag tccgccggca
      961 tcgaggggct cgcgcaggac ctcagggaaa agcccaggtg atcggcggcg tctcgtgttc
     1021 tcgggccctc gttggggggc ctggatcgcg aggcccacgg gggaaggggc ccgacacccg
     1081 gtcacgaaaa ggatgaggct cggcgagatg gacatgacct gtcacaagtg cgggtacaag
     1141 gacagctata cggcctttgc ctacctctgc aagaacggct gtcccgcctg cggcgagtct
     1201 gacctgagga ggtgccccaa gtgcggcgcc cagtgcgtgt tctcgagggc ggaggcgctc
     1261 gagggggagg agcgggagat ggagggtctc gtgggcaggc tcatggagat acacgccgga
     1321 tccacccctg aagagatcct ggaagcaaag aggatcatgg cgaggttgtc cgacatgaac
     1381 aaccggtgga acctgtcccg cctcagggag ttgctgatcc tcaaacagaa ggagttcacg
     1441 tcccaacgct gagggaaagg gggaaggggt gcaggagacg aaggtcgtga ctcaaacccg
     1501 caaggacacc aggcggctcc cgggctcgga cgggctcata ccggtaccgg ggcgcgagga
     1561 ccatttcttc ctcaaggccg aggtgatacg gctgctcgag cgggtagagc tgctgtccga
     1621 gagacaccct gtcaacgtcc tcgtcgtcgg tccgcaggga tgcggaaagt cgacgctcgt
     1681 gaggcagttc gcagcccggt tcaacaggcc gctcgccgtc ttccaggtcg ggatcctctc
     1741 cgaggcgggc cagctcttcg gggagcacag gctcgaggag ggtcgtacct acttcaatga
     1801 attcctcttt cccaaggcgg tccgcacccc ccggtgcgtc atccacctgg aggagatcaa
     1861 ccggccggaa caccccaagg cgctcaacat gctcttgagc ctcctggccg aggacaggtc
     1921 ggtgtggata gacgagatcg gccgtgttga cgtggcaccc ggcgtcgttt tcttcgccac
     1981 catgaacgag ggtgaggagt tcgtcgggac cgagatgctc gacgcggcgc tcagggatcg
     2041 gttctacatc gtcgcgctgg actacctgcc gccggaggtg gagacgcagg tcctgcgcct
     2101 caaggcgaac ctcggcgagg cggacgccct caccgtcgtg aacgtggcga accggttgcg
     2161 gacgaaccgg aacgacccgg tgaccgtgtc caccaggcac accctcatga tcgccgagat
     2221 ggtcggcttg ggcgcctctg ccaaggacgc cttcgtcagc gggctccagg tcggccgtga
     2281 cacgctcgag accgtcctgc tctcggttca cctcgagggg aggctgccgg gcggggagga
     2341 aaaggatgag taccacgagt attgacggca cgaaggaacc ccagggccat gggcccctgt
     2401 ccgcctcgtg gaggaggaac ctctccagcg tcgaggcgct cgagatggcc aacgtcctca
     2461 gggcgttgcg caaggtcgtg ggccacctgg ggaccaatgc gcccgaggtc gagtacgcag
     2521 ggttcccctc cacaggggcg aactcgatct tcgtcgaccc ggccagggtc atggggacct
     2581 acccggtgcc gcacgagacc tcggacgtgc tcatcggcga ggtggtccac gaggcgatcc
     2641 tcggcatcga gtggacggat cgtgtacgct cgctcctcgt caagcacatg tcgggcctcg
     2701 gcatgattga gctcgtgaag ttccagcgca tggtctccac gggcgagcac atatacgccg
     2761 acgccgttct cgatgggacg gtgctcgggg cgtacgcgtc caaggcgagg cgccatgcct
     2821 tgagaaactc ctgggcggtg cgttcccggg aaaaatcggg tttttcgtac gacaccctca
     2881 tgctctcgtg gatccaggcg tcgtttctcg acggcggctc gcataccgtt cacaaggggc
     2941 tcgcgcggtg catggacgtg ctcttggacc tcaccgcatc gctcgcccgc atagccggct
     3001 cgtccagggg ccaggtcttc aggtgcgaag aacgatcctc cctctacctc aaggcgtggt
     3061 cggcgctcga gccgctcgtg agggacgtgc ctgtcatcga caggagactc gtctggtcac
     3121 cgggcgatga tccctctccc gcacgaaagg cccctggaca ccgcttgggt acccaggctc
     3181 atcccgcctg cacggaagac ctcgtccgca gggtggagct ggaactcgcc aggtcatcga
     3241 gcgacctgac gccgcttgtg agggcggccc tggggggcga cgacggggat gtggtcccca
     3301 tctccaggtg ggactaccgg gtgagcgcgc accccgtggt cgacagggcg atggccgcga
     3361 ggatcgcggc cgtgttcagg atctacgcgg agagaaggcc gcggttgtcc agggggcttg
     3421 ccgcaggcag ggtcgatggc cggaggctct acagggtgcc cctctcgggg aggtgtttct
     3481 tcgagcgcga ggcgcacaag gagatggact ggaacatcac gctgttgtgc gatgcgagcg
     3541 gctccatgag gggggccaag tggaagaccg tcgagagcac gatcgcgacc atctgcaagg
     3601 cgatcgaggc ctaccggggc atgttgaacg catatgccta cttcgagaac gacggggtgt
     3661 gcatgctctc ctcgctcctg gaaggggggc gcctgttcgc ggtgcctcct ggaggaagga
     3721 cggcgtctgg gcaagccatc atcgcggcag gcctcctcat gccctcgtcg aacaggcgca
     3781 ggctcctcat ccacgtcacc gacggggagt ccaactacgg ttgcagcgtg cagaaaggca
     3841 tcgatttctg ccgcgcccag ggaatccacc tc
//
LOCUS       DAOSCR010000215         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14882_length_3866_cov_3.577276, whole genome shotgun sequence.
ACCESSION   DAOSCR010000215 DAOSCR010000000
VERSION     DAOSCR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14882_length_3866_cov_3.577276"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..1584
                     /locus_tag="PLT69_06410"
     CDS             454..1584
                     /locus_tag="PLT69_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOJ14105.1"
                     /translation="MRSVFIHSSKFDTYSLGSDHPFKPYKATMVLELCRRYGLMDRPW
                     MKVHEATPATEEVMAEYHAKEYIDALKSCNTGYFDFTLLHYGLGTGDNPVFEGVFDFS
                     LLVLGATMAGVDYIVRGEADTVFSPVGGLHHGGYGHAEGFCYVNDIVIAIQRLLKSGY
                     RRILYVDIDAHHGNGVQDAFYDTDEVLFFSVHQNGETLYPGTGFENETGEGKGKGYTV
                     NLPLPEYTDDEVYVRAFREIFPRLVEAYRPQCVVAQIGLDTLKRDPLTNLRLTNNGYC
                     EVMEDIRKSCPVILALGGGGYSLPDVVRGWTLAWAILNGIEPEDTFAGSVGAGVYGHG
                     FGELKDPPHRVSDALRKAVDKYVDDKVSYIKSNVFPMLGITP"
     gene            1645..2070
                     /locus_tag="PLT69_06415"
     CDS             1645..2070
                     /locus_tag="PLT69_06415"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOJ14106.1"
                     /translation="MADEKAEGRKGYTFVDKRGKDAPEAPGVKGPDSGAGEVRSERQD
                     DPRRRQPEGQPGQRRPSVDFTTLVMSLASAAMMSMGRVADPATGAVRKDLAVAQQNID
                     IIALLKEKTRGNLTAEEERIVDQVLYELRLTFVEAMRER"
     gene            2072..3565
                     /locus_tag="PLT69_06420"
     CDS             2072..3565
                     /locus_tag="PLT69_06420"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOJ14107.1"
                     /translation="MKKMVRFRSRGRGIPSLVGCLAAAFVMIAGIPSPGMAEGGVFWK
                     EGAAGSRSKLELPAFSRLAKEVTPTVVNIRTTRRVAGRDTLRGINPPPGYDEWFDDFF
                     KKFFNNIPENELQQKSLGSGFIISADGYVLTNNHVIAGADEIIVSLADKEEFKAGVVG
                     TDDKTDIALIKINSTRKDFPVAVLGNSDELEIGEWVIALGNPFGFGHTLTQGIVSAKS
                     RVIGAGPYDNFIQTDAAITPGNSGGPLIDMRGQVVGINTAIVASGQGIGFATPINMVK
                     EILPELRETGEVARGWLGVSIQEVSPEIARAVGLDEPKGAMVTTVYPGDPAEKAGIQP
                     GDIIVGVNGKAVEDPRALTRMIGSLKPGSKVDLSVFRDKKVLNLRAVLSKRTDERVTK
                     LGKKTTPGGGVRDRIGLVVTDITPDVQREHDISEKEGVLVIDVDPKGPASDGRILKMD
                     IIKEVDRKAVRNLDDYLKALSSKKKGQAVLLLLMRGSSPLYVAVDIE"
     gene            3562..>3866
                     /locus_tag="PLT69_06425"
     CDS             3562..>3866
                     /locus_tag="PLT69_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOJ14108.1"
                     /translation="MRLFCPAKINLYLKVIGKRPDGYHELRTIMVPLAFGDDLEIEPA
                     PSGIHLEAPGCGCAENENLAWKAASLFLARTRVTGGVRIRIHKRVPVGSGLGGGSSDA
                     "
BASE COUNT          747 a         1180 c         1323 g          616 t
ORIGIN      
        1 gcacgccgcg tggagcatct gatcaccgaa ggggcacctc tccggtccgg ccttccccgg
       61 gcgcgcccga ccatgtgcgg cggtatggaa cgtgaggccg catccggcca tccccgggcg
      121 aacacaacgc gccgatcctg ggaagtgggt gcgctgctct ccccgtgggc gaggccgtag
      181 gaggccgagg ccggtttccc tgccccggcc tgagtctctg aggccgccat gctttctccc
      241 cgggcacgaa ggcgcctgtg cgctttcgag ccgcgccgtc gtccgccaac aatcgccggt
      301 gcctacggcg aaggggcgga ttgcaccgcg ggcctcacac cgttttcccc gtcttttcct
      361 tcatgagctc tttccacccc ccgtgttctt ctggtagact cgatgcttgt ctgctctttc
      421 gtgcagcgag acgagaaaag gggagcaccg aagatgagat ccgtttttat ccactcgagc
      481 aagttcgata catacagcct gggatccgac catcccttca agccgtacaa ggccaccatg
      541 gtcctcgagc tctgcaggag gtacgggctc atggacaggc cgtggatgaa ggtgcacgag
      601 gcgacgcccg caaccgagga ggtcatggcc gagtaccacg ccaaggagta catagacgcg
      661 ctcaagtcgt gcaacaccgg ctatttcgac ttcaccctgc tccactacgg gttggggacg
      721 ggtgacaacc cggtcttcga gggcgtgttc gacttctcgc tcctggtgct gggcgcgacc
      781 atggcagggg tcgactacat cgtcaggggc gaggccgaca cggtcttcag ccccgtgggc
      841 gggctccatc acggcgggta cgggcacgcg gagggcttct gctacgtgaa cgacatcgtg
      901 atcgcgatac agaggctttt gaagtcgggc taccgaagga tcctctacgt ggacatcgac
      961 gcgcaccacg gcaacggggt gcaggacgcg ttctacgaca cggacgaggt gctcttcttc
     1021 tccgtgcacc aaaacggcga gaccctctac ccgggcaccg gcttcgagaa cgagacgggc
     1081 gagggaaagg ggaagggcta cacggtgaac ctccccctgc ccgagtacac ggacgacgag
     1141 gtctacgtga gggccttcag ggagatcttc ccgagactgg tggaggccta caggccccag
     1201 tgcgtggtcg cccagatcgg cctcgacacc ctcaagaggg acccgctcac gaacctgagg
     1261 ctcaccaaca acgggtactg cgaggtgatg gaggacatac ggaagagctg cccggtgatc
     1321 ctggccctgg gcgggggcgg atacagcttg ccggacgtgg tcaggggctg gacgctcgcc
     1381 tgggcgatcc tgaacgggat cgaacccgag gacacattcg cggggagcgt cggggccggg
     1441 gtgtacggac acggcttcgg cgagctcaag gacccgccgc accgcgtctc ggacgccctg
     1501 aggaaggccg tggacaagta cgtcgacgat aaggtaagct acatcaagtc gaatgtgttc
     1561 ccgatgctcg gcatcactcc gtgagggccg ggctcggttg aatggcgaag gaaggttcat
     1621 ccatgcgtat gtcgaggtga tgccatggct gacgagaagg ctgagggcag gaagggatac
     1681 acgtttgtag acaagcgggg caaggacgcc cccgaggcgc ctggcgtgaa ggggcccgac
     1741 tccggggccg gggaggttcg gtccgagagg caggatgacc cgaggcggcg ccagcccgag
     1801 ggccaacccg gccagaggag accctccgtg gatttcacga cgcttgtcat gagccttgcg
     1861 agcgccgcga tgatgagcat gggcagggtc gccgaccccg cgacaggggc tgtccgcaag
     1921 gatctcgccg tggcgcagca gaacatagac atcattgcgc tcctcaagga gaagacacgg
     1981 gggaacctca cggcggagga ggaacggatc gtggaccagg tgctctacga gctcaggttg
     2041 acgttcgtcg aggccatgag ggagcgctga gatgaagaag atggttcgtt tccgctcgag
     2101 gggaagggga atcccttccc tggtgggttg cctcgcggcg gcgttcgtga tgatcgccgg
     2161 catcccctcc cccggcatgg ccgagggcgg tgtcttctgg aaggaggggg cggccgggtc
     2221 ccgcagcaag ctcgagctgc cggcgttttc caggctcgcc aaggaggtga cgcccacggt
     2281 cgtcaacatc cgcaccacga gaagggtcgc gggcagggac acgctcaggg ggatcaaccc
     2341 gcctcccggc tacgacgagt ggttcgacga cttcttcaag aagttcttca acaacatccc
     2401 ggagaatgag ctgcagcaga agagccttgg gtcgggcttc atcatctccg ccgacgggta
     2461 cgtgctcacc aacaaccacg tgatcgcggg cgcggacgag atcatcgtgt cgcttgccga
     2521 caaggaggag ttcaaggccg gcgtcgtcgg caccgacgac aagacggaca tcgccctcat
     2581 caagatcaat tcaacgagga aggatttccc ggtcgccgtc ctcgggaact cggacgagtt
     2641 ggagatcggc gagtgggtga tcgcgctcgg caacccgttc ggtttcggac acacgctcac
     2701 ccagggcatc gtgtccgcga agtcccgcgt cataggcgcc gggccctacg acaacttcat
     2761 ccagacggat gcggccatca ccccgggcaa ctccgggggc cccctcatcg acatgcgcgg
     2821 ccaggtcgtg ggcatcaaca ccgccatcgt ggcgtcgggc cagggcatcg ggttcgccac
     2881 gcccatcaac atggtgaaag agatcctgcc ggagctcagg gagacgggcg aggtggcgcg
     2941 cgggtggctc ggggtgtcca tacaggaggt ctcgccggag atcgcgaggg cggtggggct
     3001 cgacgaaccg aagggcgcca tggtgacgac cgtctacccc ggggatcccg ccgagaaggc
     3061 cggcatacag ccgggagaca tcatcgtcgg cgtcaacggg aaggcggtgg aggacccccg
     3121 ggccctcacc aggatgatcg gcagcctcaa gcccggctcg aaggtcgacc tgtcggtctt
     3181 cagggacaag aaggtcctca atctgagggc tgtcttgagc aagaggacgg acgagcgcgt
     3241 cacgaagctc ggcaagaaga cgacgcccgg aggcggtgtc agggaccgga tcggcctcgt
     3301 ggtgacggac atcacgcccg atgtgcagcg cgagcacgac atctccgaaa aggagggggt
     3361 gctcgtcatc gacgtcgacc cgaagggccc ggcgtctgac ggcaggatcc tgaagatgga
     3421 catcatcaag gaggtcgacc gcaaggcggt caggaacctc gatgactacc tcaaggccct
     3481 ctcctccaag aagaagggcc aggccgtgct cctgctcctg atgcgagggt catcgcccct
     3541 ctacgtggcg gtggacatcg agtgagactc ttctgtcccg caaagatcaa cctctacctc
     3601 aaggtgatcg gcaagaggcc ggacgggtac cacgagctcc gtaccatcat ggtgcccctg
     3661 gctttcggcg acgacttgga aattgagcca gctccctcgg gtatccatct cgaggcgccg
     3721 ggatgcgggt gcgccgagaa cgagaacctc gcgtggaagg cggcgtccct cttcttggcg
     3781 cgcacgcgcg tgacgggcgg ggtccggata cggatacaca agagggtgcc ggtgggctcc
     3841 gggctcggcg gcggcagcag cgacgc
//
LOCUS       DAOSCR010000216         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14896_length_3863_cov_2.856092, whole genome shotgun sequence.
ACCESSION   DAOSCR010000216 DAOSCR010000000
VERSION     DAOSCR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14896_length_3863_cov_2.856092"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PLT69_06430"
     CDS             <1..1542
                     /locus_tag="PLT69_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14109.1"
                     /translation="VKAVPAKLDRICEDIGLGKDPPGIFSLVNKAGARLAQTAIRLQQ
                     DREASSSARSRSEILVSLLKELNDTSRVTDALTRATEALFRAGIVKGVIGGLAIDGGK
                     LVYERRLETGDQFIKLTDAEAKALVSERGYAAGVSLPSGIFVYLDLVDAACFDDHDFV
                     VSIVASIAAALRRIQAETALGREKNRLREALVASSRERRKVEELMELNRELVEASVVG
                     LCLVDANLRVRVENPESARLRKLIGLGGVSLGEEGFEGADEGAGALKDAVARGEAFSV
                     VVESRGRSVRCSAQPVARSRMTLLILEDITSELDEQRRTLAYAKMSVVGSLAASMAHN
                     MKSPIGAIHGFANMIREDLRLGRITVRRGDAEDKDLPDMLANIVTASENLLKIVNQLL
                     NFTRKWESPVVEVRLDEFVTNAFMLLEAQANNAGVTLVNEAEPVTARLKADALEQVLI
                     NILMNAVTASPRGAGVTVKAALKGDGVEVAVSDRGIGMTEEQASRVFDPLYSSWPMKT
                     GMVWA"
     gene            1649..>3863
                     /locus_tag="PLT69_06435"
     CDS             1649..>3863
                     /locus_tag="PLT69_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14110.1"
                     /translation="MMEQRILIVEDDLVFRNYLYQVLKYDYDVKAVPGPLEALQALGQ
                     ARYALMITDLRMPDMDGRALVEKVHAEIDPDIMIIVITAFEDDWPMDQAMASHVFRYL
                     RKGGFLPSELKQDVDKALEIRASMKGLQASGQPSRRPEAQPGGDITAEECQKRLSKLT
                     EEVKDLIVWLDADCRCTYANAQARRLLGPFVEGPLEGSPIPWEKFVHPEDIGVLERVR
                     ALAGGGESRHEGEVRIVTGPGEVRWLSYTVFLEHGQEGRLSAVDIVAEDVTDRKTAEE
                     ELKNANKKLQEFNERLSGGVSRKIRELMESEERYKHIVEDSGDIIFSLDAQARIISMN
                     RRGLQTLGLELEQVKGMHCSSFISDEASMQRLAEITRAMDANDRHGPFDLAIETRQGR
                     RIYRADLVRIGERSRPEVVCVARDISDEIAKEKRLTLLAAIEHFSPDAIVGLDTQGRI
                     LSWNTGAQTMFEWTEGEVAGREISMLVKDDARHRIQAVLHEVREKGVLRDIEGVWQTR
                     SGRLLDVKVTVNALKDEAQEVFGYSAIVVDQTEKKKMETALIQAERLAAMGRLAASIA
                     HEINNPLYGIRSCLNHVLAAGRGEVDHQFVRLALKETDRIAELIRNMKTFYQPHDGGV
                     SETDITELLREVFILNRKYLEENLVKLVFTPSGPMVVECIPEQIKQVFINIVTNAVEA
                     MPDGGELRVDCASVDDGGAVEVVFRDTGVGISREDLPQIFDMFYSKKPSVKGVGLG"
BASE COUNT          774 a         1148 c         1365 g          576 t
ORIGIN      
        1 gtgaaggccg tccccgcgaa actggaccgg atctgcgagg acatcgggct gggcaaggat
       61 cccccgggga tcttctcgct cgtcaacaag gcgggggcga ggctcgcgca gaccgcgata
      121 cggctccagc aggacaggga ggcgtcgagc tccgccagga gccggtcgga aatcctcgtc
      181 tctctcctca aggaactcaa cgacacctcg agggtgaccg acgcgctcac gcgcgccacg
      241 gaagccctct tcagggcggg gatcgtgaag ggggtcatag gcgggctcgc catcgacggg
      301 ggaaagctcg tctacgagcg ccgcttggag accggcgatc agttcataaa actcaccgac
      361 gcggaggcca aggcactcgt gagcgaacgc ggatacgccg caggcgtgag cctcccgtcg
      421 gggatcttcg tgtacctcga cctcgtcgat gccgcatgtt tcgacgatca tgacttcgtc
      481 gtgtccatcg tcgcttccat agcggcggcg ctgaggagga tccaggccga gaccgcgctc
      541 gggagggaaa agaaccgcct gagggaagcg cttgtggcct cgtcccgcga gcggcggaag
      601 gtcgaggagc tcatggagct caaccgcgaa ctcgtcgagg cctccgtggt cgggctgtgc
      661 ctggtggacg cgaacctgcg cgtgagggtc gagaaccccg agtccgcacg gctcaggaag
      721 ctcatcggcc tcggcggcgt atcgctcggc gaggaagggt tcgaaggcgc cgacgagggt
      781 gccggcgccc tgaaggacgc ggttgcccga ggagaggcgt tcagcgtggt ggtcgagagc
      841 agggggaggt ccgtcaggtg ctcggcccag cccgtggcga gatccaggat gacgctcttg
      901 atcctcgagg acatcaccag tgagctcgat gaacagaggc ggaccctggc gtacgcgaag
      961 atgtccgtgg tgggttccct cgcggcctcc atggcccaca acatgaagag cccgataggc
     1021 gcgatccacg ggtttgcgaa catgatcagg gaagacctca ggctgggcag gatcacggtg
     1081 aggcgtgggg atgccgagga caaggacctg ccggacatgc tcgcgaacat cgtgaccgcc
     1141 tccgagaacc tcctcaagat cgtgaaccag ctcttgaact tcacgcgcaa gtgggagagc
     1201 cccgtcgtgg aggtgcgcct cgacgagttc gtgacgaacg ccttcatgct gctcgaggcg
     1261 caggcgaaca atgcgggcgt caccctcgtg aacgaggcgg agccggtgac ggcgcgcctg
     1321 aaggcggacg cgctcgagca ggtgctcata aacatcctca tgaacgccgt gacggcgtct
     1381 cccagaggcg ccggcgtgac ggtcaaggcg gcgctcaagg gcgacggggt ggaggtggcc
     1441 gtgtccgacc gggggatagg catgaccgag gagcaggcct cgagggtctt cgacccgctc
     1501 tactcgtcgt ggcccatgaa gaccggcatg gtctgggctt gagccttgcc aggcagatcg
     1561 tggagtcctt gggcggcacg atcgaggtga gctcgaagct tgcacagggg accacattca
     1621 cagtatggat accccagggc gtggccgaat gatggaacag cgcatcctca tcgtggaaga
     1681 cgatctcgtt ttcaggaact acctctacca ggtcctgaag tacgactacg acgtcaaggc
     1741 cgttcccggg ccgctggagg cgctccaggc gctcgggcag gcccggtacg cgctcatgat
     1801 caccgacctg cgcatgcccg acatggacgg gagggccctc gtcgagaagg tccacgccga
     1861 gatcgatccc gacatcatga tcatcgtgat caccgccttc gaggacgact ggcccatgga
     1921 ccaggccatg gcatcccacg tgttcaggta tctccgcaag ggtgggttcc tgcccagcga
     1981 gctcaagcag gacgtggaca aggccctgga gattcgcgcc tccatgaagg ggctccaggc
     2041 gtcgggtcag ccctccaggc ggcccgaggc gcagcctggg ggtgacatca cggcggagga
     2101 atgccagaag aggctctcca agctcaccga ggaggtcaag gacctcatcg tctggctgga
     2161 cgccgactgc aggtgcacct acgccaacgc ccaggcgagg cgtctcctcg ggcccttcgt
     2221 ggagggcccg ctagagggtt cgccgatacc gtgggagaag ttcgttcacc ctgaggacat
     2281 aggcgtcctg gagcgcgtgc gggccctggc cggaggggga gagtcccgcc atgagggcga
     2341 ggtgcggatc gtgaccgggc cgggcgaggt gagatggctg tcctacacgg tattcctgga
     2401 acacggccag gagggccgtc tctcggccgt ggacatcgtg gccgaggacg tgacggacag
     2461 gaagaccgcg gaagaggagc tgaagaacgc caacaagaag ctccaggagt tcaacgagcg
     2521 cctctcggga ggggtgagca ggaagatccg cgagctcatg gagtcggagg agcggtacaa
     2581 gcacatcgtc gaagactcgg gggacatcat cttctcgctc gacgcgcagg cgcggatcat
     2641 ctccatgaac cgcaggggcc tccagaccct cggcctcgag ctcgagcagg tcaaggggat
     2701 gcactgctcc tcgttcatat ccgacgaggc gtccatgcag cggctcgccg agatcacgag
     2761 ggccatggac gcgaacgacc ggcacggccc gttcgacctg gccatcgaga cgaggcaagg
     2821 gcggaggatc tacagggccg acctcgtcag gataggcgag aggagcaggc cggaggtcgt
     2881 ctgcgtggcc agggacatca gcgacgagat cgccaaggag aagcggctca cgctcctggc
     2941 cgcgatcgag cacttcagcc cggacgccat cgtggggctc gacacccagg gcaggatcct
     3001 ctcctggaac acgggcgcac agacgatgtt cgagtggacc gagggcgagg tcgcgggcag
     3061 ggagatctcc atgctcgtca aggacgatgc caggcacagg atacaggcgg tgctgcacga
     3121 ggtcagggaa aagggggtgt tgcgggacat cgagggggtg tggcagacca ggtccggccg
     3181 cctgctcgac gtcaaggtga cggtcaacgc cctcaaggac gaggcacaag aggtcttcgg
     3241 gtactcggcc atcgtggtgg accagaccga gaagaagaag atggaaacgg cgctcatcca
     3301 ggccgagcgc ctcgcggcca tggggaggct cgccgcgagc atcgcgcacg agatcaacaa
     3361 ccccctctac ggcatcagga gctgcctgaa ccacgtgctg gcagcgggca ggggggaggt
     3421 tgaccaccag ttcgtgaggc tcgccctcaa ggagacggac aggatcgcgg agctcatcag
     3481 gaacatgaag acgttctacc agcctcacga cgggggcgtg agcgagacgg acatcacgga
     3541 gctcctcagg gaggtcttca tcctgaacag gaagtacctc gaggaaaacc tggtcaagct
     3601 cgtgttcacg ccgtcggggc cgatggtcgt cgaatgcatc cccgaacaga tcaagcaggt
     3661 cttcatcaac atcgtgacca acgccgtgga ggcgatgcca gacggcgggg agctcagggt
     3721 cgactgtgcg agcgtcgacg acggcggggc cgtcgaggtc gtgttcaggg acacgggcgt
     3781 cggcatctcg agggaggacc tcccccagat cttcgacatg ttctactcca agaagccgtc
     3841 ggtcaagggc gtgggtctcg ggc
//
LOCUS       DAOSCR010000217         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14970_length_3849_cov_3.321824, whole genome shotgun sequence.
ACCESSION   DAOSCR010000217 DAOSCR010000000
VERSION     DAOSCR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14970_length_3849_cov_3.321824"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..1153
                     /locus_tag="PLT69_06440"
     CDS             428..1153
                     /locus_tag="PLT69_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOJ14111.1"
                     /translation="MILSILARSLLVQSAWNFKGMQNIGFTYVLAPGLRHLLGDRADE
                     AVRRSITFFNTQPYMAPTLAGVCLHLYEKGEEDKIRKFIPSLSGSLAALGDTFFWAKL
                     KPILALVLVTCALSGAYLGMVLALLAYNAAHLWIMTWGFFKGYRHGIEGALVVGRTIS
                     VDSTHHLGLVMAFIAGITLGMTVLGRIGPQAFLGPSGGLLAAVSTAIALFAAYAAFRW
                     LRLDILWFIYGVFTLSVIWTMLK"
     gene            1150..1416
                     /locus_tag="PLT69_06445"
     CDS             1150..1416
                     /locus_tag="PLT69_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ14112.1"
                     /translation="MRQRRVTITNKLGLHARAAALFTKQASKFSSKVEVIKDHLRVNG
                     KSIMELLTIAGIQGTEITIRAEGSDEELAVETLVNLVKDGFGER"
     gene            1416..3185
                     /gene="ptsP"
                     /locus_tag="PLT69_06450"
     CDS             1416..3185
                     /gene="ptsP"
                     /locus_tag="PLT69_06450"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOJ14113.1"
                     /translation="MTDGQVHILHGVGASAGIAIGQAVVIDMRKIERYPKLKIPEDRV
                     EDELRRFDEALALSQKQVAEAISALEAHNVIREHVLILEAHLLMLKDPALVDRVRTLI
                     ATEFINAEWALKRAIEEIEKAMDAVDDAYLRSRVADAAFVAERLMRNLTGRKTGSFHH
                     AHNSIIVAHDLSPADTAMLNKGMVLGFATDVGGMTSHTAIIAHSLEIPAVVGLENASS
                     VVNPGDTIILDGISGVLIVNPSESQILEYQNRARSYLSMEIKLKEKAKEPAVTRDGKR
                     IVVKGNLEFKEEVKTVLDHGAEGIGLYRTEFLYLVRKDIPTEEDHFEAYRSVVQAVHP
                     YNTVIRTLDLGGDKFYSALNTIFSERNPVMGLRAIRLCLKETGIFKTQLKGILRASHY
                     GDTSIMFPMISGIDELHQAKEILEETKRELKIQGIPFDEDIRVGIMVEVPSAAVIADL
                     LAKEVDFFSLGTNDLIQYSLAIDRGNEFVNYLYEPLHPAVLRLIKFTVDAAHNAGIEV
                     SMCGEMAGREIYTPILLGMGIDSLSTNAYAISHVKEMARKINIEKCRKIVEHLFEMRK
                     ASEIYEFMTREIMENTHDLFDLA"
     gene            3209..3679
                     /gene="smpB"
                     /locus_tag="PLT69_06455"
     CDS             3209..3679
                     /gene="smpB"
                     /locus_tag="PLT69_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOJ14114.1"
                     /translation="MGDKGQQGHKVLCANRTARFDYEILETLEAGIVLAGPEVKSLRN
                     GGGSIKDSYAQVKDGEMFLVNFRISPYVQASIHNQDPMRPRKLLLHRREISRLSVKVK
                     EKGLTLVPLSLYLKGNRVKVELGLAKGKRSHSKKEAIKERDIRREMDREVKSWR"
BASE COUNT          787 a         1225 c         1186 g          651 t
ORIGIN      
        1 cccgggcagg gtgtgcaccc ccgccatcgt cgttccatgg tcctctccct cgaccccgca
       61 cgaacatgcg gatcgagggc gcgccttagt ctcacaggcc gcccctttct cgtgaaggac
      121 acgggtcttg ggggaggacg ggctctctct aaggcgtgtg caggccgcaa ccggcaccag
      181 ggggttcgac accgaggcgg ggatcacgtc attcgtgcat tcttcccgcg cgaggtgggc
      241 tccctcgtgc agccaggtca cccggccctg ccccgccgcg atcattccgc gcgaggggga
      301 gccaccgctt ctatgtcccc acccccgccg gggcccggcc tggaagagac ccctgatcct
      361 gtctcgggcc gaattgacgt gcggggaggc ctgtgttaca tgtctatgcg tgcgtctacc
      421 ccgccgcgtg atactctcca tcctcgcaag atcgctgctg gtgcagtccg catggaactt
      481 caagggcatg caaaacatcg gcttcacgta cgtgctggcc ccgggcctca ggcacctcct
      541 cggggacagg gcggacgagg ccgtcaggcg ttccatcacc ttcttcaaca cccagccgta
      601 catggcaccc acgctggcag gcgtgtgcct tcacctctac gagaagggcg aggaagacaa
      661 gatccggaag ttcatcccct ccctctcggg gagcctggcc gccctgggcg acaccttctt
      721 ctgggcgaag ctcaagccga tcctcgccct ggtcctcgtg acatgcgcct tgtccggcgc
      781 ctacctgggc atggtcctgg cccttttagc ctacaacgcc gctcacctct ggatcatgac
      841 gtggggcttt ttcaaaggct accgacacgg cattgagggc gccttggtcg ttggccgtac
      901 catctcggtg gactcgacgc accacctggg cctcgtcatg gccttcatcg cggggatcac
      961 cctgggcatg acggtgctgg gccgcatcgg tccacaggcg tttctcggcc catcaggcgg
     1021 tctcctcgcg gcggtctcga ccgccatcgc gctatttgcg gcatacgcgg ctttcaggtg
     1081 gctgcgcctt gacatcctgt ggttcatcta tggagtcttc acactatccg tgatatggac
     1141 tatgctgaaa tgagacagag acgagtcacg atcaccaaca agctcggcct gcatgcacgg
     1201 gccgccgcgc tcttcaccaa gcaggcctcc aagttctcct cgaaggtgga ggtgatcaag
     1261 gaccatctga gggtcaacgg caagagcatc atggaactgc tcaccattgc ggggatccag
     1321 ggcacggaga tcaccatcag ggccgagggg agcgacgagg agctggctgt cgagaccctc
     1381 gtcaacctgg tgaaggacgg tttcggggaa cgctgatgac ggacggtcag gtgcacatcc
     1441 tccacggcgt gggcgcttcc gcgggcatcg ccatcggtca ggccgtggtc atcgacatgc
     1501 gcaagataga gcgctacccg aagctcaaga tccccgaaga ccgcgtggag gacgagctca
     1561 ggcgcttcga cgaggcgctg gcgctgtcgc agaagcaggt ggccgaggcg atcagcgccc
     1621 tcgaggccca caacgtcatc agggagcatg tcctcatcct cgaggcccac ctcctcatgc
     1681 tcaaggaccc ggcgctcgtg gaccgcgtga ggacgctcat cgcaaccgag ttcatcaatg
     1741 ccgagtgggc cctcaagagg gccatcgagg agatcgagaa ggccatggat gccgtcgacg
     1801 acgcctacct gcgcagcagg gtggcggatg cggccttcgt ggccgaacgc ctcatgagga
     1861 acctcacggg acgcaagacg ggctcgttcc accacgcaca caacagcatc atcgtggccc
     1921 acgacctctc ccccgcggac acggccatgc tcaacaaggg catggtcctc ggcttcgcca
     1981 ccgacgtagg gggtatgacc tctcacacgg ccatcatcgc ccattccctc gagatacccg
     2041 ccgtggtcgg gctcgagaac gcctcgagcg tcgtgaaccc cggcgacacc atcatcctgg
     2101 acggcatctc cggggtgctc atcgtgaacc cttccgagtc gcagatcctc gagtaccaga
     2161 acagggcaag gtcctacctg agcatggaga tcaagctcaa ggagaaggca aaggaacccg
     2221 ccgtcacgag agacgggaag cggatcgtgg tcaaggggaa cctcgaattc aaggaggagg
     2281 tcaagaccgt cctggaccac ggcgccgagg gtatcgggct ctaccggacc gagtttctgt
     2341 acctcgtcag gaaggacatc cccacggaag aagaccactt cgaggcctac cggtcggtgg
     2401 tgcaggcggt ccacccctac aacaccgtga tacggacgct cgacctcggc ggggacaagt
     2461 tctactcggc gctcaacacc atcttcagcg agcgcaaccc ggtcatggga ctcagggcca
     2521 tacggctctg cctcaaggag acgggcatat tcaagaccca gctcaagggg attctcaggg
     2581 cgagccacta cggcgacacg tccatcatgt tccccatgat atcgggcata gacgaactcc
     2641 accaggcgaa ggagatcctc gaggagacga agagggagct caagatccag ggcatcccgt
     2701 tcgacgagga catccgcgtg ggcatcatgg tcgaggtgcc ctccgcggcg gtgatcgccg
     2761 acctcctcgc aaaggaggtg gacttcttca gcctgggcac gaacgacctc atccaatact
     2821 ccctggccat cgacaggggc aacgagttcg tcaactacct gtacgagccg ctccacccgg
     2881 cggtcctccg tctcatcaag ttcacggtgg acgccgcgca caacgcgggg atcgaggtgt
     2941 ccatgtgcgg ggagatggcc ggccgagaga tctacacgcc gatccttctg ggcatgggca
     3001 tagactcgct ctccacgaac gcgtacgcca tctctcacgt gaaggagatg gccaggaaga
     3061 tcaacatcga gaagtgtcgg aagatcgtgg aacacctctt cgagatgagg aaggcctcgg
     3121 agatctacga gttcatgacc cgcgagatca tggagaacac ccacgacctg ttcgacctcg
     3181 cctgattccg acgggaggac gccggcccat gggagacaag ggacagcagg ggcacaaggt
     3241 cttgtgcgca aaccgcactg cgaggttcga ctacgagatc ctggagaccc tagaggccgg
     3301 catcgtgctg gcgggcccgg aagtcaagtc gttaagaaac ggcggcggga gcataaagga
     3361 cagctacgcc caggtgaagg acggcgagat gttcctggtc aacttccgca tcagccctta
     3421 cgtgcaggcg tccatccaca accaggaccc catgaggccg cgcaagctcc tgctgcaccg
     3481 gcgcgagata tcgaggcttt ccgtcaaggt caaggagaag gggctcaccc tcgtgccgtt
     3541 gagcctctac ctcaagggaa accgtgtcaa ggtcgagctc ggcctcgcca agggcaaaag
     3601 gagccacagc aagaaggagg ccatcaagga gcgtgacatc aggcgcgaga tggacaggga
     3661 ggtgaagtct tggagatgac gatggacgat atggacttcg aacgcatctt gaggcgctcg
     3721 ctcgccaagg gtggagactt cgccgagatc tacgtggagc acaggctggg cacatccttc
     3781 gtctgcgaat ccggcaccgt cgagaagtac ctggcgaccg acgaatccgg cgtctcgatc
     3841 cgcgtggta
//
LOCUS       DAOSCR010000218         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14983_length_3847_cov_3.664557, whole genome shotgun sequence.
ACCESSION   DAOSCR010000218 DAOSCR010000000
VERSION     DAOSCR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14983_length_3847_cov_3.664557"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..413
                     /locus_tag="PLT69_06460"
     CDS             219..413
                     /locus_tag="PLT69_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14115.1"
                     /translation="MHPPAAGEHATNPAFQSLFFTFTESTSSGRRYTAEHSTKYWREP
                     CENAGERVDVNRGLPGQAGW"
     gene            complement(734..810)
                     /locus_tag="PLT69_06465"
     tRNA            complement(734..810)
                     /locus_tag="PLT69_06465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:Pro,seq:cgg)
     gene            complement(1223..1996)
                     /locus_tag="PLT69_06470"
     CDS             complement(1223..1996)
                     /locus_tag="PLT69_06470"
                     /inference="COORDINATES: protein motif:HMM:NF023351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HOJ14116.1"
                     /translation="MRRMVCLFTLVFTLGWSGSLPAAAPEVIWAGGGLKLSDNSNPCF
                     FADTFVLLQQDDGMSLFTQPVLTLKGGEVGFDLGLGGRSLLMGDQLAGGWNVFFDYTS
                     DNSHKRFGAGLEVFHPNATGHLNFYLPVSNEEGGQEALAGMDFQLGVPLPNAAFISIR
                     PGFYYYSGEDDENKSGISLALRIQPIRPLALTIGGRNDALQSGRDTSEIFVRVDFAIP
                     FDRLGRDLFQPYRPEYPLDVRTLLDARVVREEFITYERK"
     gene            complement(2025..3062)
                     /locus_tag="PLT69_06475"
     CDS             complement(2025..3062)
                     /locus_tag="PLT69_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14117.1"
                     /translation="MEFIKVLKKSRFLDPQRGDEEVVRYSEVRYDPLTSHSSRILDFP
                     VRSVERADLSALVEASKAFCPFCPEVVEVVTPKFHPELAGRSRYSRGEALCVPNAFPY
                     DENGAVTVMTRSHYLALKDFTREIIADAIGCCLMYLVDVAQRQPDMVFQSVNWNYMPQ
                     AGGSIVHPHLQVAASSSPTNYYAEVIPALERYRSRHGTDYWTDLVEEEGRQGRRNIGT
                     TGRVSWVTAFAPMGIFDVLGVIEGVRCPGDIGEDVLADLSSGIVRVLRYIDSLNMHSL
                     NMSLYFHLGSPLFTPHVRICPRANIPPFDTSQINYMGMLHRESLTILKPEDVCDGIRR
                     MWEGQGGDVHD"
BASE COUNT          701 a         1243 c         1228 g          675 t
ORIGIN      
        1 tcggtgatgt aaaccgtgga ttgaaggatg cggcccatgt cggaaccggc ttcgacccgg
       61 cgctttggcg caggacacgg ccatgcacgg gccttggtcg agtccggcga gaaccgaggc
      121 tgggtgttcc ccccagccca cgcgatcgat ggtggacgcg gcggccgcga cggatccagg
      181 cgcaccgccg cagggcgtcc cccccaggcc gcgagaccat gcaccctccg gcggccgggg
      241 agcatgctac gaaccctgcc ttccagtcgc tcttcttcac cttcaccgag tccacgtcgt
      301 cgggcaggcg gtacaccgcg gagcactcca ccaagtactg gcgcgagccc tgcgagaatg
      361 ccggggagag ggttgacgtg aaccggggac ttccagggca agccggatgg tgaacgagga
      421 aggcgtaagc tcgtcaggcc tgcagatcgc cggggaccgg gctttcatat ccccggtgac
      481 cgcttatgcc atggcgaacg ttgagagacg gcgggaaatc cgctcgggta aggtgatccg
      541 caccgaccag gaaagcccgg ggttattcat gggcttgttt tcgcgaggct cagcgatcga
      601 catcattggg aaaaatggcc gggaagcagg ggcttgaacc ccggatcatt gtgggtgtgc
      661 ctggcttttc gggcggtccg ttgcactcgc tgggctgtcc tcgaagatca actggttgat
      721 agtgtcatga aaatggtcgg gaagcgggga tttgaacccc ggaccccagc gtcccgaacg
      781 ctgtgctcta ccagactgag ccacttcccg agaccgagga ctatctatag aaagctaagg
      841 cgcggaagtc aagaaacttc ctgagcacac cgcctgcctc ttcccacgat ctcctggaac
      901 acggcccccg cctccccacc ctgaagggga ggaccattcc ttgaacttgc tcgttgagga
      961 cgctggaacc caagcccttc gcggtgatcc atgccgcagg tggggaaaga ggcctccatg
     1021 tgcgcaacgg cccgggccgg ttgtatccag gcttcctgtg gcgttcagca aacggcagaa
     1081 agcgatttct gcgtgatcaa cggcccgggg gcatgagcag agacaacacc gcagggatac
     1141 tgtctctcaa ggccctcggg cgagccggct tctcgtctcg tcgcacccgc tgcatcccgc
     1201 tcgccctcgg ggcgggcacc ccctacttcc gctcgtaggt gatgaactcc tcgcgcacca
     1261 cgcgggcgtc caggagggtg cgcacgtcca gcgggtattc gggcctgtac ggctggaaga
     1321 ggtcccttcc gagcctgtcg aagggtatgg cgaagtccac cctgacgaag atctcgctcg
     1381 tgtccctgcc ggactgcagc gcgtcgttcc tgccgccgat ggtcagcgcg agcggacgta
     1441 tgggctggat gcggagcgcc agggatatgc cgctcttgtt ctcgtcgtcc tcgccggagt
     1501 agtagtagaa gcccggccgt atggagatga acgcggcatt cggcaagggg acgccgagct
     1561 ggaaatccat gcctgcaaga gcctcctgtc ccccctcctc gttggatacc ggcaggtaga
     1621 agttcaggtg gccggtcgcg ttcgggtgaa agacctcgag ccccgcgccg aacctcttgt
     1681 gggagttgtc cgaggtgtag tcgaagaaga cgttccagcc ccctgcgagc tggtcgccca
     1741 tgaggaggga cctgccgccg agacccaagt cgaacccgac ctcaccgccc ttgagcgtca
     1801 gcacgggctg ggtgaagagc gacatgccgt cgtcctgctg cagcagcacg aacgtgtctg
     1861 cgaagaaaca gggattggag ttgtccgaca gcttgagacc gcctcccgcc catatgacct
     1921 caggcgccgc ggctgggaga gagccgctcc acccgagcgt gaacaccaac gtgaacaagc
     1981 acaccatcct cctcattgca ccacctcctg tgtctctcat cgacttaatc atgcacgtcc
     2041 ccgccctgcc cttcccacat gcgcctgatc ccgtcgcaga catcctcggg cttcaagatg
     2101 gtgaggctct ccctatggag catgcccatg tagttgatct ggctcgtgtc gaaggggggg
     2161 atgttggccc tgggacagat cctcacgtgc ggggtgaaca gggggctgcc gaggtggaag
     2221 tacagggaca tgttcaggct gtgcatattg agcgagtcga tgtacctgag gaccctgacg
     2281 atccctgagg acaggtcggc cagcacgtct tcgccgatat ccccgggaca acgcacgccc
     2341 tcgatcacac cgagcacgtc gaagatgccc atgggcgcga aggccgtcac ccatgagacc
     2401 ctccccgtgg tgccgatgtt cctccggccc tgccttcctt cttcttccac caggtcggtc
     2461 cagtaatcgg tgccgtgccg gctccggtac ctctcgagcg ccgggatgac ctcggcgtag
     2521 tagttggtgg gtgagctgga agccgccacc tggagatgcg ggtgcacgat ggacccgccc
     2581 gcctggggca tgtagttcca gttcaccgac tggaagacca tgtccggctg cctctgcgcg
     2641 acatcgacga ggtacatgag gcagcagcct atggcatcgg cgatgatctc ccgggtgaag
     2701 tccttgaggg ccaggtagtg gctcctggtc atcacggtca ccgcgccgtt ctcgtcgtag
     2761 gggaacgcgt tcggcacgca cagggcctcg cccctggagt agcgcgagcg gcctgcgagc
     2821 tccgggtgaa acttgggtgt gacgacctcc accacctccg ggcagaaggg acagaacgcc
     2881 ttggacgcct ccaccagcgc cgagaggtcg gccctctcca ccgacctgac agggaagtcc
     2941 aggatgcggc tcgagtggct ggtcaaaggg tcatacctca cctcggaata gcggacgacc
     3001 tcctcgtccc ctcgctgggg gtccaaaaaa cgtgatttct tgagcacttt gatgaattcc
     3061 accaccttca cctttacacc atggcaggga gataatcaac ccgggagggc cttgcgcgat
     3121 cgaggcgcgc aggcactgga gggcctgccc ggaaaggccg gcgcgcaggg tcgcccgggc
     3181 cggcccattc ggctggctgt accgtgttcc gccatagcgg catgcgcccc tgtgccgtga
     3241 ggttggcgcc ccctggggcc gaagtgggcg cccaccaggg gcgacctcgg cgtccccacc
     3301 gaacgggcgt gtggtcccgg atgcacgtca cgccgcatcg cccgccggca cggcgcgggc
     3361 acgagagaga cgacgacgtc gattcatcct cgtccatccc cagcccgacg tctcttgggc
     3421 atgggggttg cctctttgcg tgccccgccc ggtccacggt ctgaagagct cgagatgaac
     3481 cccaaccccc tgccgtcgat tcgggcaagg agacgctccg tgcacgaaag gtcccactcc
     3541 ggagggctca ccgcaaccat tggcctttcc cgagggagac gcgcatacca cgtgagagaa
     3601 gatcccttgg ccccgccggg gcctgtcggc cgggtcatcc tcgagaaggc accagcgccg
     3661 ctttggctgc tccagcgtgg tcgcgcgacg gatcgggccc tgtgcatcct tgatcgcggc
     3721 aagcccagct tgtataatcc tcccccggtc tacgatcaga agagttcgag aggaacctca
     3781 tgggccctgc cgcgaattcg ggcaaggaga cgctccttgc acgcaagacc atggtcgagg
     3841 agcagat
//
LOCUS       DAOSCR010000219         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_14988_length_3846_cov_4.296492, whole genome shotgun sequence.
ACCESSION   DAOSCR010000219 DAOSCR010000000
VERSION     DAOSCR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14988_length_3846_cov_4.296492"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2646)
                     /locus_tag="PLT69_06480"
     CDS             complement(<1..2646)
                     /locus_tag="PLT69_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14118.1"
                     /translation="MNRTLKRLAVACLAVTIIAFAVMAAGTYVISRSPAQVASAIERQ
                     VKDALDMPVAIGKARFEWKRGPRVILNAVVVDQPGALKLEIPSVTAYLSIPRLMLGQA
                     RLSKVRLVNPKGWADLDNLQSFLKSESARERPVLVIWKGRVRISAWGVDLELDELSGR
                     ITKDWLNLRARTLGGRVLLETDLEAPGKLIFDAYDVDLSAVDASVRGRAFVNTALSMR
                     EDGTTGTASLKLKGLDTPWTRRPVDWVNASAAIGLSRGYASATDIVVGTPVVEIKGKA
                     AVRLPAGKGSWEDAQAQVDVSSGWFSHDELVSYLPLEKFPDWFADLLGRQVRSGRSRF
                     VRAVYGGPVKALRSGVDLLDRVRVVQELDGQSFTGRPGGETITGITGRVVYAGGDIRF
                     ERLRGTAGSSVLDRVDIAFPGALRPYMRVGVNAELDMPALDFMRAWNTAVRDTGVSDL
                     LGPVSGVKAGRVVYRLSTYYDEQAKDPFSFKGGVEVRDADFSWAGARVSRFQGSLKAH
                     RFGEPLVVKASGLFSGTRVRSFDLRLASPFKRNESRFALEADRLAPQAGVSLEKPRLR
                     AEGSGRGGNLSGSVHLAAEAVVAPAGGGRTVRVEGVQAASDFTGTLDSGLSLSFTKVA
                     VRTRCGSLSGTAGSTKDGLSASLSGAVDLGCMSLTGAGGVQRLSGAVKGSFRFASRGT
                     TASLWADMDLDGVELPLETGAVRLDGRLGVEPGRLAMRRLAARVEDATFVLDGTLESS
                     PGKRYFEGSVDVTGLHAGEKQAGGGLQGLWGPLSGRADLRLHACSFHGFSVDEATALA
                     RLEGGRLVLDRFETSAYEGRATGRIELAPGGDAPFAAALRLSGARLDRVLKAALGSSS
                     ASGTLDLEARLEGDAGGVEGTVEVNA"
     gene            2802..>3846
                     /gene="serS"
                     /locus_tag="PLT69_06485"
     CDS             2802..>3846
                     /gene="serS"
                     /locus_tag="PLT69_06485"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ14119.1"
                     /translation="MLDIKFIRQHEDLVQQALANRGSSLTLDTLLEKDAERRTLIQKT
                     EDLKARRNQFSQEIARSKGKGDDLEARKAEMRKISEEIKDLDARIREVESAMEAFVLE
                     IPNIPHESVPVGEGSQDNQVVRTWGERPVFDFPPKDHWDVGEALGVLDFKRAAKITGA
                     RFTVSWGLGSRLERALINFMLDVHTQEHGYTEVWTPFMTNRDSMTATGQLPKFEEELF
                     RTDDYYLVPTAEVPVTNLYREEVLAEEDLPVKLVCYTPCFRREAGSYGKDTRGLIRQH
                     QFNKVELVRFAHPERSFTDLEELTADAEDILKKLGLHYRVVVLCTGDLGFSSAKTYDL
                     EVWMPAQGVYREIS"
BASE COUNT          664 a         1383 c         1229 g          570 t
ORIGIN      
        1 cgcgttcacc tcgaccgtgc cctcgacgcc gccggcgtca ccctcgagcc tcgcctcgag
       61 gtcgagcgtg ccgcttgcgg acgaggagcc gagcgccgcc ttgaggaccc tgtcgagcct
      121 ggcccccgag agcctgagag ctgccgcaaa cggcgcgtca cccccgggtg cgagctcgat
      181 ccttcccgtc gccctgccct cgtaggcgct cgtctcgaac ctgtcgagaa cgagcctgcc
      241 gccctcgagg cgcgcgagcg ccgtggcctc gtccacggag aagccgtgga atgaacacgc
      301 gtgcaggcgc aggtcagccc tgccggaaag gggaccccac aggccttgca ggcccccgcc
      361 tgcctgcttc tcgcccgcat ggagaccggt cacgtcgacc gagccctcga agtacctctt
      421 tcccggcgaa gactcgagcg tcccgtcgag gacgaacgtc gcgtcctcga cccgggcggc
      481 gagccggcgc atggccaggc gtccgggctc cacgccgagc ctgccgtcca gcctcaccgc
      541 gcctgtctcc agggggagtt ccacgccgtc gagatccata tccgcccaca gggacgccgt
      601 cgtccccctc gaggcgaagc ggaacgaccc cttgaccgct cccgacagtc tctggacacc
      661 ccctgcaccg gtgaggctca tgcaccccag gtccacggcc ccggacaaag aggcgctcag
      721 gccgtctttc gtcgagcccg ccgtgcccga gagggagccg cacctcgtcc tgacggccac
      781 cttcgtgaac gacaggctca ggccgctgtc cagggtgccg gtgaagtcgg acgccgcctg
      841 caccccctcc acgcggaccg tcctgccacc ccctgcggga gcgaccaccg cctcggccgc
      901 caggtgcacg gatcccgaga ggttcccgcc cctgccgctc ccctccgccc tgagcctcgg
      961 cttctcgagt gacacgccgg cctggggcgc cagccggtcc gcctccagcg cgaagcgcga
     1021 ctcgttcctc ttaaagggcg aggccagcct gagatcgaag gacctgaccc tcgtgcccga
     1081 gaagaggccg gaggccttca cgacgagcgg ctcgccgaac cggtgggcct tcagggagcc
     1141 ctggaagcgg ctgacccggg cccccgccca ggagaagtcg gcatcgcgga cctcgacgcc
     1201 ccccttgaac gagaaggggt ccttggcctg ttcgtcgtag taggtggaga gcctgtacac
     1261 gaccctgccg gccttcacgc cggataccgg gccgagcagg tcgctcaccc cggtatcacg
     1321 cacggccgtg ttccacgcgc gcatgaaatc gagcgccggc atgtcgagct cggcgttcac
     1381 gccgaccctc atgtagggcc tgagcgcgcc cgggaaggcg atgtccaccc ggtcgaggac
     1441 ggaggacccc gcggtccccc gcaggcgctc gaaccggatg tcgccccccg cgtagacgac
     1501 cctgcccgtg atgccggtta tggtctcgcc gccgggcctg cccgtgaagc tctgtccgtc
     1561 cagttcctgg accacgcgga ccctgtccag caggtccacc cccgagcgca gggcctttac
     1621 gggcccgccg tagacggcgc ggacgaatct cgaccgtccc gagcgcacct gcctgccgag
     1681 gaggtctgcg aaccagtccg gaaacttctc gagggggagg taggagacga gctcatcgtg
     1741 ggagaaccag cccgacgaca cgtccacctg ggcctgcgcg tcctcccacg agcctttccc
     1801 tgcgggcagc ctcacggcgg ctttgccctt gatctccacc accggtgtgc ccacgacgat
     1861 gtcggtggca gaggcatatc ctcgcgagag gccgatcgcg gctgaggcgt tcacccagtc
     1921 cacagggcgc ctcgtccagg gggtgtcgag tcccttgagc ttcaaggacg cggtgcccgt
     1981 ggtcccgtcc tcgcgcatcg acagggctgt gttcacgaac gccctgcccc gcaccgaagc
     2041 gtcgacggcg gagaggtcca cgtcgtaggc gtcgaagatc agcttgcccg gggcctcgag
     2101 gtcggtctcg aggagaaccc tgccgcccag ggtgcgcgcc ctgaggttga gccagtcttt
     2161 cgtgatgcgt ccgctcaact cgtcgagctc caggtcgacg ccccaggcgg agattctcac
     2221 gcggcccttc cagatgacca gcacgggccg ttcgcgcgca ctttcggact tgaggaagga
     2281 ctgcaggttg tccaggtcgg cccagccttt cgggttcaca aggcggacct tggacagccg
     2341 cgcctgaccc agcatgagcc tcgggatcga gaggtaggcg gtcacgctcg ggatctcgag
     2401 cttcagtgcg cccggctggt ccacgaccac cgcgttgagg atcacgcggg gccccctctt
     2461 ccattcgaac ctcgccttgc cgatggccac gggcatgtcg agcgcgtcct tgacctggcg
     2521 ctcgatcgcg gaggccacct gggcgggcga cctcgatatg acgtaggtcc ccgccgccat
     2581 gaccgcgaag gcgatgatcg tcactgccag gcaggcaacg gcgagccgtt tgagcgtcct
     2641 gttcatcccc cgggcaccaa ccccctgtca cgaacgaccg gcgacaccac ctccatagaa
     2701 catgcggcga cccgaacgca agccctcaag aacacccgta aagagcacgc cttccgagtg
     2761 ccgtccttga cgatgcaccc accgggtggt acattcgtga catgctcgac ataaaattta
     2821 tcaggcaaca cgaggacctg gtgcaacagg cgctcgcgaa cagggggtcg agcctgaccc
     2881 tcgacaccct cctggaaaag gacgccgaac gaagaaccct catccagaag accgaagacc
     2941 tcaaggcgag gcgcaaccag ttctcccagg agatcgcgcg ctccaagggc aagggcgacg
     3001 acctcgaggc ccgaaaggcg gagatgagga agatctccga ggagatcaag gacctggatg
     3061 cgaggatcag ggaggtcgag tccgcgatgg aggcgttcgt cctggagatc cccaacatcc
     3121 cgcacgagag cgtccccgtg ggcgaaggct cccaggacaa ccaggtggtg cgcacctggg
     3181 gcgagaggcc cgtgttcgac ttccccccga aggaccactg ggatgtcggc gaggccctgg
     3241 gcgtgctcga cttcaagcgt gcggccaaga tcaccggcgc ccgcttcacc gtgagctggg
     3301 gcctgggctc gcgcctcgag cgcgcgctca tcaacttcat gctcgacgtg cacacgcaag
     3361 agcacggcta caccgaggtg tggacgccgt tcatgaccaa ccgcgacagc atgacggcca
     3421 cgggccagct ccccaagttc gaggaggagc tcttcaggac cgacgactat tacctcgttc
     3481 ccaccgccga ggtgccggtc accaacctct accgcgagga ggtgctcgcc gaggaagacc
     3541 tgccggtcaa gctcgtgtgc tacaccccct gtttcaggag ggaggcgggc tcctacggca
     3601 aggacacgcg cgggctcatc aggcagcacc agttcaacaa ggtggagctc gtccgtttcg
     3661 cgcaccccga gcgctccttc accgacctcg aggagctcac ggcggacgcg gaggacatcc
     3721 tgaagaagct cgggctgcac tacagggtcg tggtgctgtg cacgggcgac ctcgggttct
     3781 cgtcggccaa gacctacgac ctcgaggtct ggatgccggc gcagggggtg taccgggaga
     3841 tatcct
//
LOCUS       DAOSCR010000220         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15129_length_3822_cov_3.243164, whole genome shotgun sequence.
ACCESSION   DAOSCR010000220 DAOSCR010000000
VERSION     DAOSCR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15129_length_3822_cov_3.243164"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PLT69_06490"
     CDS             <1..1336
                     /locus_tag="PLT69_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14120.1"
                     /translation="VSCREKEDAAVTGIPEAFRGEPLAVGVCVGDPSSGPGLGVFQEK
                     GLRAAHRAFQPSMGRGITLVFKKGIRNEADWDRAVGSLVREERVSGVISCDVPVPPGR
                     MKEIVSGCEIPFVSICRRADGRAPDPAPNELRICTPPDVRARACACFISREVKAKRVA
                     LVVNGADGEAVRLASLFAEELSAMGAHLVDVVVKDGGSERTSGLERLSSLRVDAVFLP
                     VDADAALGLVEEIRRRDASVPVLVAGPPAPGRLTARAAGILDGVYVQSDFAEDEVNTP
                     RGRAFLSFWKGSEGPGGPVGLQEAVGAEAYLLMVDVLSRSRGAEVVKAVGDMSRRRGE
                     LIVCDGVAPSGLIHRQVLFGRFSKRFSDPVFIRPVARVAVVGSDPVADVGAQELLGDH
                     GEVRLVRAARDGRLHLGHLFHPDLGGLLDRLVYVLHQLDRGRDVDLVGRVGR"
     gene            complement(1425..1649)
                     /locus_tag="PLT69_06495"
     CDS             complement(1425..1649)
                     /locus_tag="PLT69_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14121.1"
                     /translation="MEVKNIFMNLIMRTYERQHASNANCLCGGEVKNGAANPEDAITI
                     SETATRRFFRDIMENSLQKGLNEVMTNENI"
     gene            2103..2852
                     /gene="flgF"
                     /locus_tag="PLT69_06500"
     CDS             2103..2852
                     /gene="flgF"
                     /locus_tag="PLT69_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOJ14122.1"
                     /translation="MDQGIYTAASGAIAMEERLNVISHNIANVNTVGYKKDHILYREF
                     TKILDTSMLAQGQYRVIPVDVVATRPTVDLTQGVSMKTDNPLDVAINGEGFFVVMTED
                     GSRYTRAGSFMISEDNNLVTPQGHRVQGKGGDITIDTTTGDGQVVIGPDGRISYDGTE
                     VDSLQVVNIPAEALVRAGGNLFAVRDGHVPDPVENPSVSQGYLERANVEPVTEMVELI
                     TTQRAYEAYQRMIRSINDAYSNSIRNVGAVS"
     gene            2889..3670
                     /gene="flgG"
                     /locus_tag="PLT69_06505"
                     /pseudo
     CDS             2889..3670
                     /gene="flgG"
                     /locus_tag="PLT69_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
BASE COUNT          681 a         1170 c         1303 g          668 t
ORIGIN      
        1 cgtatcgtgc cgggagaagg aggatgccgc cgtgacgggg atacccgagg ccttcagggg
       61 ggaaccgctc gcggtgggcg tatgcgtggg agacccttcg tctggacccg gcctcggggt
      121 cttccaggag aaagggctca gggcggccca cagggccttc cagccctcca tgggaagggg
      181 gataacgctc gtcttcaaga agggcatacg caacgaggcc gattgggacc gggctgtcgg
      241 ctccctcgtg agggaagaac gcgtaagcgg cgtcatctcc tgcgacgtac cggtcccccc
      301 ggggcgcatg aaggagatcg tctcgggctg tgagatcccg ttcgtctcga tctgccgccg
      361 ggcagacgga agggcgccgg atcccgcccc caacgagctg aggatatgca ccccgcccga
      421 tgtcagggcg cgcgcatgcg cctgcttcat atcccgggag gtcaaggcga aacgagtggc
      481 cctggtcgtg aacggggcgg acggcgaggc ggtgaggctc gcaagcctct ttgccgagga
      541 gctgtccgcc atgggcgcgc acctggtgga cgtggtggtc aaagacggcg gaagcgaacg
      601 cacttccggc ctcgagcggc tctcgtctct acgggtcgat gccgtcttcc tcccggtgga
      661 cgcggacgcc gcgctcgggc tcgtcgagga gatccggcgc agggatgcat cggtgcccgt
      721 cctcgtggcc ggtccgcccg cccccggtcg tctcacggcg agggcggcgg ggatccttga
      781 cggcgtgtac gtgcagagcg acttcgccga ggacgaggtg aacacccccc ggggacgggc
      841 gttcctctcg ttctggaagg ggagcgaggg gcccggcggc cccgtgggac tgcaggaggc
      901 ggtgggcgcg gaggcttacc tgctcatggt cgacgttctg tcacgatcga gaggggccga
      961 ggtggtcaag gccgtgggcg acatgtcgag gcggcggggg gaactcatcg tctgcgacgg
     1021 ggttgcgccg tcgggcctca tccacaggca ggtcctcttc ggcaggttct cgaagaggtt
     1081 ttccgacccg gtcttcatca ggccggtcgc gcgcgtggcg gtagtcggtt cagatcctgt
     1141 cgctgatgtt ggggctcaag agcttctggg cgaccacggt gaggtccgcc ttgtacgtgc
     1201 cgctcgtgat ggccgcctcc acctgggcca ccttttccac ccggacctcg ggggcctcct
     1261 tgatcgcctg gtgtatgtcc tgcatcagct tgaccgcggt cgagatgtcg accttgtcgg
     1321 acgtgtgggc cgttgagttc gcctgagact gctgcgaggt gcgcacaggc tgtacgccct
     1381 gcgccgaacc ggtcgtgtat gccttgatgg cgtcgatggg ggtgttatat attttcattg
     1441 gtcatgacct cgttcaggcc tttctggagg ctgttttcca tgatgtccct gaagaacctc
     1501 ctggtcgccg tctccgatat ggtgatggcg tcttcagggt tcgcggcgcc gttcttgacc
     1561 tccccgccgc acaagcagtt cgcgttcgat gcgtgctggc gttcgtaggt cctcatgatg
     1621 aggttcatga agatgttctt gacctccatg cgagctcctt gcgcgtagtc aaccgtctgc
     1681 cattctgtat cggatctgcg tgaaaattct ttagcattct ttcgttgcac agccaaagga
     1741 aaaggtgcgc ccggccgccg ggcgcaggct gcacgtgcgc acgtgcgttc acgggaggga
     1801 agcgcccccg gcacgggtga agcgagagat cgacccggtg aggtcccggt gcaggagccc
     1861 ccgtccccgg cacgggtgag acgataccat ggcgaaaggg gcgggagggg gctcggcgat
     1921 acacgggccg cacggagggc gacccatccg gccgcgagga ggaccacccg acctgttgtt
     1981 cccgcatcct tgatcgaggc gggaaaaatt ttcccgcacc gggcgggcct gctcgtgaga
     2041 tcgtgcggct tttcccccgt ggcatcaccc ttgctgggaa ggctcgagga aaggagggcg
     2101 gtatggacca gggcatctac acggcggcat cgggcgccat cgccatggag gagaggctga
     2161 acgtcatctc ccacaacata gcgaacgtga atacggtcgg ttacaagaag gaccacatcc
     2221 tctaccggga gttcacgaag atcctggaca catccatgct ggcccagggg cagtaccggg
     2281 tcatcccggt ggacgtcgtc gcgacgagac caacggtgga cctgacgcag ggcgtttcca
     2341 tgaagacaga caaccccctg gacgtggcga tcaacggcga gggtttcttc gtggtcatga
     2401 ccgaggacgg ctcccggtac acgagggcgg ggtcgttcat gatctccgag gacaacaacc
     2461 tcgtcacgcc ccagggacac cgcgtccaag gcaagggcgg ggacataacc atcgacacga
     2521 cgaccggcga cggacaggtc gtgataggcc ccgacgggag gatcagctac gacggcaccg
     2581 aggtggactc gctccaggtg gtgaatatcc cggcggaggc gctggtgcgt gccggcggga
     2641 acctcttcgc cgtcagggac ggccacgtgc cggatcccgt tgagaatccc tcggtctcgc
     2701 aagggtatct cgagagggcg aacgtggagc ccgtcaccga gatggtggag ctcatcacca
     2761 cccagcgcgc ctacgaggcc taccagagga tgatcaggtc catcaacgac gcctactcga
     2821 actccatccg caacgtgggc gcggtctctt gacgtaactt ccggagaaaa cgaggaaagg
     2881 agaaacccat gatacgagcc atgtggacct cggcgacggg catgagcgcg caggagatgc
     2941 tggtcaacac catcgccaac aaccttgcga acgtgaacac caccgggttc aagaagagca
     3001 gggtcgactt cgaggatctg ttctacgcac ctacagcggc aagggcaccg agacctcgga
     3061 ggggggcacc gtgcccgtgg gcatagaggt cggtctcggc gtgaggcccg tctcgatcca
     3121 gaagatcttc acgcagggcg attacctgca gacgggcaac agcctcgacc tggccatcga
     3181 cgggaagggg ttcttcaaga tcctctccgg cggcgaggag gtgtacacgc gcgacggcgc
     3241 gttcaagatc gacagcgacg gctacatcgt gaactcgcgt ggggagaggc tccagcccga
     3301 gttcacggtc cctccccaga ccgccaccat cacggtgtcc aagtcgggcg agatcaactg
     3361 cctgtcgaac gccggggaga cgctcgccac cgggaacatc ccggtgtaca tcttccccaa
     3421 tcccgccggg ctcaggtcgc tgggcatgaa ccagtacgcc cccacccccg cctccggtga
     3481 cgccatcgag ggcacgcccg gcaccgagaa cttcggttcc atctcctcgg gtttcctcga
     3541 gatgtccaac gttgacgccg tggaggagat ggtgaacatg atcgccgcac agcgggccta
     3601 cgagctcaac tccaagtcca tcaaggcctc ggacgacatg ctccagaccg tgaccggcct
     3661 catgaggtag cttggcatgg gtaccgtgag ggtggccatg tgcgtggcgg tgtttctcat
     3721 ggcggccacg cgtgcatccg gtgaccaggg tgtgacgggc gcatgcctgg aacaggtatg
     3781 cggcccttct tccctggcaa ccgccgttga tgccgggcgg ca
//
LOCUS       DAOSCR010000221         3819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15151_length_3819_cov_2.995218, whole genome shotgun sequence.
ACCESSION   DAOSCR010000221 DAOSCR010000000
VERSION     DAOSCR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15151_length_3819_cov_2.995218"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLT69_06510"
     CDS             <1..610
                     /locus_tag="PLT69_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14123.1"
                     /translation="VKDTIVKIGEGGEILIKGPQVMKGYYNKPDDTKAAFTHDGWFMT
                     GDIGRMDERGCLYITDRKKDIIVTAGGKNIAPQNIENTLVADLYIEQAVVIGEGRKYL
                     SALIVPNFKELGEYAKKQGISFNTNEDLVRKPEIVAFYEAKIRDLMKDYARVEQIRKF
                     TLLGREFSIDTGELTPTLKIKRKVVNENYKEAIEAMYKDDRD"
     gene            1536..3749
                     /locus_tag="PLT69_06515"
     CDS             1536..3749
                     /locus_tag="PLT69_06515"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOJ14124.1"
                     /translation="MFRSIKKRDGREVEFEASKITAAIAKAGRATGEFGEETAHMLML
                     KVLSIAERAIDGVPTVEAIQDIVEEVLLQTTFRKTAKAYIIYRDQHAKIREIKAGMGV
                     DLIDQYLERLDWQVNENSNMSFSLQGLNNYLSSEISKIYWLNKIYPPEVRDAHASGDF
                     HIHDLNIISVYCVGWDLYDLLLKGFKGAPGKVESKPAKHFRTALGQIVNFFYTLQGEA
                     AGAQAFSNFDTLLAPFIRYDGLDYDQVKQALQEFIFNINVPTRVGFQTPFTNVTLDLK
                     VPSHYADTGVVIGGEIQDAVYGEFQKELDMFNKAFVEVMANGDAKGRVFTFPIPTYNI
                     TADFDWDNPHLKGLWEMTAKYGVPYFSNFVNSDMDPADARSMCCRLRIDNRELFKRGG
                     GLFGSHPLTGSIGVVTINLPRLGYLSTSVDDFLERLGRLIDIAGTSLEIKRKILESFT
                     ENNLYPYTKFYLADMKEKAGEYWKNHFSTIGIVGMNEACLNLFGETIATARGKGFALE
                     VADFIRERLVQLQERTGNNYNFEATPAEGTSYRLARMDKKRYPDIVCANEAEYRKGYE
                     PFYTNSSQLPVNYTDDIFEALDLQDEIQTKYTGGTVLHIFAGERVEDPEVIKHLVRKI
                     CNTYRLPYFTFSPTFSVCPAHGYIAGGHPQCPTCGEPTEVYSRVVGYLRPVAQWNNGK
                     KEEFSLRKTFRVEKAVSAEDIVSAQVYRIRPPKPGERCVAQREAGATETASTVEA"
BASE COUNT          816 a         1170 c         1219 g          614 t
ORIGIN      
        1 cgtgaaggac accatcgtga agatcggcga gggcggggag atcctcatca aggggcccca
       61 ggtcatgaag gggtactaca acaagcccga cgataccaag gccgcgttca cccacgacgg
      121 ctggttcatg accggcgata tcgggcgcat ggacgagcgc ggctgcctgt acatcacgga
      181 caggaagaag gacatcatcg tcacggccgg cggcaagaac atcgcccctc agaacataga
      241 gaacacgctg gtggccgacc tgtacataga gcaggcggtg gtgataggcg agggcaggaa
      301 gtacttgagc gcgctgatcg tgcccaactt caaggagctg ggagaatacg cgaagaaaca
      361 ggggatatcg ttcaacacca acgaggatct cgtgaggaag ccggagatcg ttgccttcta
      421 cgaggcgaag atcagggacc tcatgaagga ctatgcccgt gtcgagcaga tcaggaagtt
      481 cacgctcctg gggcgtgaat tcagcatcga taccggcgaa ctcacgccga cgctcaagat
      541 caagaggaag gtcgtgaacg agaactacaa ggaggcgatc gaggccatgt acaaggatga
      601 cagggactga ggccttcccg gccctcgtcc ttgtgcctcc ggccggtctt gcggccttcg
      661 gggggagcgc gtgaacgaca cgccctccct ccggttctca cccccctgga tgacgcaccg
      721 acgacgggga ggcggggtcg gcgcaccgaa ggggtaacgt gcgcgccgga tgccgtctcg
      781 ggcatgcatt tgctgacctg gaccctgccg cccagataga agagctggga actcgtcccg
      841 ggcctcagcg tctcaggtgt gcatctgacg accttgaccc tgttgcggtc cttgggtttc
      901 gcggtccgga gagggcgacg acagggctga cccttggaag ccgtcgacgc actggagccc
      961 gggaaggggc ggtctctccc ggcgtgcctc aaagacgcgc gcaaaggacc aagaacgatt
     1021 tctcgggcga tgcaggccgg cacccatgag ccgggtgccg gtctccctgc ctgaggcgac
     1081 acccccgcct cgagggaccc acccgcaggg cgcgccctgt caggagcgcc gaggtccatg
     1141 acaggtcggc ctcacccggg taacgctttt cccggcagaa ggccggaccg ccggctgcat
     1201 gcagggctgc gcctgaaggt catgccgcca tgcaggggag gaccctgggc attgcctcgg
     1261 gccggcgctc gatgccgccc catgcagtga gtcccgcctg cccggcgtgg ggaaacccgg
     1321 gcacgagccg cctggcaggc acacccactt ttcccccttg cttttcccgg agaatggcta
     1381 tatatggtat ttttgtgtgg atgatacgct atatatagta tcttcccagg ggggaggccc
     1441 ggccccgggc aggccgccag gccgcagccc tttgggggcc ggcaagggga tgcggattgg
     1501 ttctcggcaa tcgtgaaata ctgcaaggag ggggcatgtt caggagcatc aagaagcgag
     1561 acggtcgtga ggtggagttc gaggcttcga agataaccgc agccatcgcc aaggcgggga
     1621 gggccacggg cgagttcggg gaggaaaccg cgcacatgct catgctcaag gtcctctcga
     1681 tcgccgagag ggccatcgat ggcgtgccca cggtggaggc catacaggat atcgtagagg
     1741 aggtcctcct ccagaccacc ttccggaaga ccgccaaggc gtacatcata taccgtgacc
     1801 agcatgccaa gatccgcgag ataaaggccg ggatgggcgt cgacctcatc gaccagtacc
     1861 tcgagcggct cgactggcag gtgaacgaga acagcaacat gtcgttctcc ctccagggtc
     1921 tcaacaacta cctttcctcc gagatcagca agatctactg gctcaacaag atctaccccc
     1981 ccgaggtgcg ggacgcccac gcgagcgggg atttccacat ccacgacctg aacatcatct
     2041 ccgtgtactg cgtcggttgg gatctctacg acctcctcct caaggggttc aagggcgcgc
     2101 cgggcaaggt cgagagcaag cccgccaagc acttcaggac cgccttgggc cagatcgtga
     2161 acttcttcta cacgctccag ggtgaggccg ccggcgcgca ggccttctcc aacttcgaca
     2221 cgctgctcgc ccccttcata cggtacgacg ggctcgatta cgaccaggtc aagcaggccc
     2281 tgcaggagtt catcttcaac atcaacgtgc cgacgcgtgt cggtttccag acgcccttca
     2341 ccaacgtcac gctcgacctc aaggtcccgt cgcactacgc ggacacgggg gtggtgatag
     2401 gcggggagat ccaggacgcg gtctacgggg agttccagaa ggaactcgac atgttcaaca
     2461 aggccttcgt cgaggtcatg gcgaacggcg acgccaaggg aagggtcttc accttcccca
     2521 tccccaccta caacattacg gccgatttcg actgggacaa cccgcacttg aagggtctct
     2581 gggagatgac ggcgaagtac ggggtgccgt acttctccaa cttcgtgaac tccgacatgg
     2641 acccggccga cgcgaggagc atgtgctgca ggctcaggat agacaacagg gaactgttca
     2701 agaggggcgg ggggctgttc ggttcgcacc cgctcaccgg ttccatcggc gtggtcacca
     2761 tcaacctgcc gaggctcggc tacctgagca cgagcgtgga tgatttcctc gagcggctcg
     2821 ggaggctgat agacatcgcc ggtacgagcc tcgagatcaa gcggaagatc ctcgagagct
     2881 tcaccgagaa caatctctac ccctacacga agttctacct tgccgacatg aaggagaagg
     2941 ccggcgagta ctggaagaac cacttctcca ccattggcat cgtgggcatg aacgaggcgt
     3001 gcctgaacct cttcggcgag accatcgcga ccgcacgcgg gaaggggttc gccctcgagg
     3061 tcgcggactt catccgggaa aggctcgtgc agctccagga gcggacaggc aacaactaca
     3121 acttcgaggc cacgccggcc gagggcacct cctaccgcct cgcccggatg gacaagaaac
     3181 gctacccgga catcgtatgc gcaaacgaag ccgagtacag gaaagggtac gagccgttct
     3241 acacgaactc gtcccagctc ccggtgaact acaccgacga catcttcgag gcgctggacc
     3301 tccaggacga gatccagacg aagtacacgg gcggcaccgt gctccacatc ttcgccggtg
     3361 agcgcgtgga ggacccggag gtcataaaac acctcgtgag gaagatctgc aacacctaca
     3421 ggcttcccta cttcaccttc agcccgacct tcagcgtgtg cccggcacac gggtacatag
     3481 cgggaggcca cccccagtgc ccgacgtgcg gcgaaccgac ggaggtctac tcgcgcgttg
     3541 tcgggtatct ccggccggtc gcccagtgga acaacgggaa gaaggaggag ttctccctca
     3601 ggaagacctt cagggtggaa aaggccgtga gcgcggagga tatcgtgagc gcccaggtct
     3661 atcgcatcag gccgcccaag ccaggcgagc gctgcgtggc gcagagggaa gcgggggcga
     3721 cggagaccgc ctcgaccgtc gaggcctgag acccattgcc tgaaggcatg cgtgggggat
     3781 catggatggg ggatctccgg aagccctcaa ggcaggggc
//
LOCUS       DAOSCR010000222         3805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15223_length_3805_cov_2.768800, whole genome shotgun sequence.
ACCESSION   DAOSCR010000222 DAOSCR010000000
VERSION     DAOSCR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15223_length_3805_cov_2.768800"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLT69_06520"
     CDS             complement(<1..725)
                     /locus_tag="PLT69_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14125.1"
                     /translation="MIHRRLVCAVVAASLALTWGCAAQFMQKRELSRPMVSIAMGKIQ
                     ENDIQGALIELRKAASANPTDPEVFYAYALAYWKSDKPDRAIEYVDKAIEYAPKLEVE
                     HPGLASEAYNLKGSILVTMGRYDEAAAAFKKALSDELYQTPEFTLYNLATLYLRKHDL
                     DAAMDNAQKALEKNSHYAPAWHVLSKIHIARGRPNEAIDSLKHAILEFPGYVEARWDL
                     AGLYLQMGARELAREQLTDIVQL"
     gene            complement(787..1260)
                     /locus_tag="PLT69_06525"
     CDS             complement(787..1260)
                     /locus_tag="PLT69_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14126.1"
                     /translation="MLRTDIIRKEVLKDEDIFDEKVASDMDKRKLVYDTMFARADELA
                     SMGKGVILDATFITQALRKRAAEVASRHGRTLLIQQTSCSQKYSLDKISRRTKDNYES
                     NALTPEAYLNNKKKFEPVDLDDLKRAFPDLRIVHLLVETESDSEDGWYVIGKTVR"
     gene            complement(1439..2293)
                     /locus_tag="PLT69_06530"
     CDS             complement(1439..2293)
                     /locus_tag="PLT69_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ14127.1"
                     /translation="MHSREGSDGRWTLREIFEEAHRRGLTLVSVTDHDSLGGQAQALE
                     LASGYGIGYVTGVELNVTFTHPDWTGGKPLSLDFLGYGVDISDDALNARLEELRAYRR
                     QRAAMILERINEAFARENREPLTAADMAAIEAEADGSLGRPHIARYLVAKGIVATRQE
                     AFDRYLVSCDVPKLPLGIEEASALVRGAGGRIVLAHPDDPNGTSLVQAAGSLEDRARM
                     IEETMLPFIDGIECWHTRHTERAASFYRAFADRRGLIATGGSDCHQNPVIMGSVPVPD
                     CVAGFFGV"
     gene            complement(2301..3317)
                     /locus_tag="PLT69_06535"
     CDS             complement(2301..3317)
                     /locus_tag="PLT69_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ14128.1"
                     /translation="MRDHATLVEFMSDPSSYPEPTGSVEVVQTHISWVFVCDEFVYKV
                     KKPVDFGFLDFTTLDKRRFYTEEELRLNRRFSPEVYLEVVPVSLKGGRFILGDGSYVV
                     EYALKMRRISEDCMLHRLLAGGLADEAMLGRVGLHLARIYGGIPSTEEARRYGSVETV
                     SFNVNENFEQTARYVGGPVTASQYDEIRSWSLDFLDRKQAVFDSRQKLGWIKECHGDL
                     HLQHICVDREKIFVFDCIEFNERFRYGDVASDVAFLSMDLDYNSRTDLSKAFVEGYLA
                     GSKDTGLLDVLRFYKVYRAYVRAKVTSFMLDDEGLEPPLKEEAMARASRYYALAHRYV
                     MSAN"
     gene            complement(3314..>3805)
                     /locus_tag="PLT69_06540"
     CDS             complement(3314..>3805)
                     /locus_tag="PLT69_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOJ14129.1"
                     /translation="NELLVTGHRVVAIDMNEAELREIAAANPKAAFSYVVGDATDDET
                     RSFLMPINKKYPLADLLAALKGFDMPRRRRITVEYVLLKGVNDGVEHARRLVRLLHGI
                     RAKVNLIPFNPWPGCPFEAPEPSRVLEFEAVLRDSPFTVMIRKEKGKDILGACGQLAG
                     GIP"
BASE COUNT          601 a         1212 c         1220 g          772 t
ORIGIN      
        1 tcgagctgga cgatatccgt gagctgctcc cgcgcgagct cgcgagcccc catctggagg
       61 tagagcccgg ccaggtccca tcgcgcttcc acgtagccgg gaaactcgag gatcgcgtgc
      121 ttgagggagt ctatggcctc gttgggcctc ccccgggcga tgtggatctt ggacaggacg
      181 tgccaagccg gtgcgtagtg cgagttcttc tcgagggcct tctgtgcgtt gtccatcgcg
      241 gcgtcgaggt cgtgtttcct gaggtagagg gtggcgaggt tgtacagggt gaactcgggt
      301 gtctggtaga gctcgtctga caacgccttc ttgaaggcgg ccgccgcctc gtcgtatctg
      361 cccatggtca cgaggatgga gcccttgagg ttgtacgcct cgctcgccag ccccggatgc
      421 tcgacctcga gcttgggcgc gtactcgatg gccttgtcca cgtactcgat ggccctgtcc
      481 ggtttgtcgg acttccagta cgcgagcgca taggcgtaga agacctcagg gtcggtgggg
      541 ttggccgagg ccgccttcct gagctctatg agcgcaccct ggatgtcgtt ttcctggatc
      601 ttgcccatgg cgatcgatac catgggtctc gagagttccc tcttctgcat gaactgtgcg
      661 gcgcagcccc acgtgagcgc cagggaggcc gccacgacgg cacagacgag tctgcggtgt
      721 atcatgccag gtcctccttg cagcgggtgc cgttcgggga aggcgaatca gcccgaacgg
      781 cttctctcac ctcacggtct tgccgatgac gtaccagccg tcctccgagt cggactcggt
      841 ctccacgagc aggtgcacga tcctcaggtc ggggaatgcg cgcttgagat cgtccaggtc
      901 caccggttcg aacttcttct tgttgttcag gtatgcctcg ggcgtgagcg cgttcgactc
      961 gtagttgtcc ttggtccgcc gggagatctt gtccagggag tacttctggg agcacgacgt
     1021 ctgctgtatg agcagcgtcc tcccgtggcg cgaggccacc tcggcggccc tcttcctgag
     1081 cgcctgcgtg atgaacgtgg cgtcgaggat cacgcccttg cccatggatg cgagctcgtc
     1141 ggcccgggcg aacatggtgt cgtagacgag cttcctcttg tccatgtcgg aggccacctt
     1201 ctcgtcgaag atgtcctcgt ccttgaggac ctccttcctg atgatgtcgg tcctgagcat
     1261 ggtgtagccc ttgagcctgg cgatgaccta cgtggtctcc gtcttgttcg tggcgggcag
     1321 cccgcaggcg atgatcacca tgccctcgcc gatctccctc tcaacgaatg ccttgaacgg
     1381 ctccctcaat gtggctcacc ccctcgctcg acgtgacagg ccgacagggc ctttcccctt
     1441 atacgccaaa gaagccggcg acacaatccg ggactgggac gcttcccatg atcacggggt
     1501 tctggtgaca gtcggatccg ccggtggcga tgaggccgcg cctgtcggca aaggccctgt
     1561 agaacgaggc cgcacgctcc gtgtggcgcg tgtgccagca ctcgatgccg tctatgaagg
     1621 ggagcatggt ctcctcgatc atgcgcgccc tgtcctcgag agagcccgcg gcctgcacca
     1681 acgaggtgcc gttggggtcg tcggggtgcg cgagcacgat cctgccccct gcgccgcgta
     1741 cgagggccga ggcctcctcg atgccgaggg gcagcttggg cacgtcgcag gacacgaggt
     1801 acctgtcgaa ggcctcctgg cgggtggcca cgatgccctt ggccacgagg tacctggcga
     1861 tgtggggcct gccgagggag ccgtcggcct cggcctcgat ggccgccatg tccgcggccg
     1921 tcaggggctc cctgttctct ctcgcgaagg cctcgttgat cctttccagg atcatggcgg
     1981 ccctttgcct gcggtacgcc ctgagctcct cgagcctggc gttgagcgca tcgtccgaga
     2041 tgtccacgcc gtaacccaga aagtcgaggc tcaacggctt gccgccggtc cagtccgggt
     2101 gggtgaaggt cacgttgagt tccacgcccg tgacgtaccc gatcccgtag cccgaggcca
     2161 gttccagcgc ctgggcctgg ccgccgagcg agtcgtggtc ggtcacggac acgagggtga
     2221 ggccgcgcct gtgagcctcc tcgaagatct cccggagggt ccacctcccg tcggagccct
     2281 cccgcgagtg gatgtggagg tcaatttgcg ctcatgacat agcggtgggc gagtgcgtag
     2341 taccgggaag cccgtgccat cgcctcctcc ttgagtggag gttcgagccc ctcgtcgtcg
     2401 agcatgaagg aggtgacctt ggccctcacg tacgccctgt aaaccttgta gaaccggagc
     2461 acgtccagga gccccgtgtc tttcgacccc gcgaggtagc cttccacgaa cgccttggag
     2521 aggtccgtcc tcgagttgta gtcgaggtcc atggacagga aggccacgtc ggaagccacg
     2581 tcgccgtagc ggaaccgctc gttgaactct atgcagtcga acacgaagat cttctcccgg
     2641 tccacgcata tgtgctggag gtggaggtct ccgtggcatt ccttgatcca cccgagcttc
     2701 tgcctcgagt cgaagaccgc ctgcttcctg tccaggaaat ccaggctcca ggagcggatc
     2761 tcgtcgtact gggacgcggt gacgggcccg ccaacgtacc tggccgtctg ctcgaagttc
     2821 tcgttgacgt tgaacgagac ggtctccacg ctcccgtaac gccgggcctc ctccgtgctc
     2881 gggatgcccc cgtagatacg ggccaggtgg aggcccacgc gacccagcat ggcttcgtcc
     2941 gcgagccctc cggccaggag cctgtggagc atgcaatcct cgctgatgcg ccgcatcttg
     3001 agcgcgtact ccaccacgta ggagccgtcc cccaggatga atcttccccc cttgagcgag
     3061 acagggacga cctcgaggta cacctcggga gagaacctcc tgttcagcct cagttcctcc
     3121 tcggtgtaga accttcgctt gtccagggtg gtgaagtcga ggaacccgaa gtcgacgggc
     3181 ttcttcacct tgtagacgaa ctcgtcgcag acgaagaccc acgagatgtg cgtctggacg
     3241 acctcgaccg aacccgtcgg ttcagggtag ctcgacgggt cactcatgaa ttccacgagc
     3301 gtggcgtgat ccctcacggt atacctcctg ccagctggcc gcaggcgccg aggatgtcct
     3361 tgcccttttc cttcctgatc atcacggtga aggggctgtc tctgagcacg gcctcgaact
     3421 ccaggacgcg agagggctcg ggcgcctcga agggacagcc gggccagggg ttgaacggta
     3481 tcaggttgac cttggccctt atgccgtgga ggagtctcac gagcctgcgt gcgtgctcga
     3541 ccccgtcgtt cacgcccttg aggagcacgt attccacggt gatgcgcctc cttctcggca
     3601 tgtcgaaccc cttgagcgct gcaaggagat cggcgagcgg gtacttcttg tttatgggca
     3661 tgaggaaaga gcgggtctcg tcgtcggtgg cgtcgccgac cacgtagctg aacgccgcct
     3721 tcgggttggc ggcggcgatc tcgcgcagct cggcctcgtt catgtcgatg gcgaccaccc
     3781 ggtgaccggt caccaagagc tcgtt
//
LOCUS       DAOSCR010000223         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15230_length_3803_cov_3.250534, whole genome shotgun sequence.
ACCESSION   DAOSCR010000223 DAOSCR010000000
VERSION     DAOSCR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15230_length_3803_cov_3.250534"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLT69_06545"
     CDS             <1..1241
                     /locus_tag="PLT69_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ14130.1"
                     /translation="VRSLLERDDITHLSEVMNYPGVVAGDVGLLEMISAAKRLGKPID
                     GHAPGLSGQALATYVSRGITTDHEVTSLGEAMEKISLGMRILVREGSAAKDFQALHRL
                     IERHGPMCMFCSDDIHPDDLVRGHIDSLVRRAVRMGHDPVQVLRCASLNPVRHYRMDL
                     GLLRLGDHADFLVVRDLQEFRVERAYLRGRLVAQGGTALVGRDRPEPVNRFEARRISS
                     SDIEVPARGGRMLVIEAEDGSLVTGRAVVKPRVESGLVRSDPVGDVLKIVVVNRYRDQ
                     APAVAFARGFGLASGAIASSVAHDSHNIVAVGVQDEDICAAVDAVMGGGGGIALAAGG
                     RVECLGLPVAGLMSDLEAQEVAGRFARLNDMAGSLGSPLASPFITLSFMALLVIPSLK
                     LSDRGLFDSERFAFTSLWDA"
     gene            1879..2346
                     /locus_tag="PLT69_06550"
     CDS             1879..2346
                     /locus_tag="PLT69_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOJ14131.1"
                     /translation="MDDLVAKDERMVTRDTLKDEGVPGVKAVCCETPDRPFSTSADGE
                     EGDPIDLEQALSEFDGDRDFLQGLLRAFLENVGRQIPALRRAIGTADAQEVRSLAHAI
                     KGGAGNLGAQRLAECAWAMERMAGEGGLARAHEALERLEAQHERLKRYVSERL"
     gene            2473..3750
                     /locus_tag="PLT69_06555"
     CDS             2473..3750
                     /locus_tag="PLT69_06555"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ14132.1"
                     /translation="MRVLVVDDELVSREKMRRIMAGLFGGCDEASTGQAALEAVSAAM
                     SENRPYELITLDISMPSMDGIDVLKRIRESERAENVPKDARAKIFMVTAASERETIIT
                     CIKEGCNDYITKPFSSDRVLKKLRDNGILPPEPDGAPAGAQASAAKNSLDGVIERFNR
                     GEMELPPMHLIQRKFYELTASGATLAAIADLLEKDPAISSKLIRISNSSFYRGYNENV
                     TVEQAVNRLGLIATKQTVDAVCSRALYDDVHPNYVQVVEKLWVHSLACAYACQLIAEL
                     RGIRLLEDPFTMGLFHDIGKLMLLRVIGELQRSGKDVDTDTDMLMSAVETHHGKAGAV
                     LLKKWLFPAVFVQVAELHDDLGKVLTPGRELLVVQLANIAVKSLGYGTPNAQGVQPGA
                     SEQAKSLGVDPSVMDLVGTQVKKYMDEFVEYLS"
BASE COUNT          690 a         1161 c         1332 g          620 t
ORIGIN      
        1 aggtacgctc cctgctcgaa agggacgaca tcacgcacct ctcggaggtc atgaactacc
       61 cgggcgtggt ggcaggcgat gtcggcctcc tcgagatgat aagcgccgcg aagaggctcg
      121 gcaagcccat agacggccat gcgccgggct tgagtgggca ggccctcgca acgtacgtct
      181 cccggggcat cacgaccgac cacgaggtca cctcgctcgg cgaggccatg gaaaagatct
      241 ccctcggcat gaggatcctc gtgagggagg ggtccgcagc gaaggacttc caggcactcc
      301 atcgtctcat cgagcggcac ggccccatgt gcatgttctg cagcgacgac atccacccgg
      361 acgacctggt gaggggacac atcgactctc tcgtgcgaag ggcggtgcgc atgggccacg
      421 acccggtgca ggtcctcagg tgcgcgagcc tcaaccccgt gaggcactac cgcatggacc
      481 tgggtctcct gcgcctggga gaccatgcgg acttcctcgt ggtccgcgac ctccaggagt
      541 tcagggtcga acgcgcctac ctgaggggca ggctcgtggc acaaggcggc accgccctcg
      601 tgggacgcga ccgtcccgag cccgtcaaca ggttcgaggc ccggcggata tcgtcttccg
      661 acatcgaggt gcccgcccgc ggcgggagga tgctcgtgat cgaggcggaa gacggctcgc
      721 tggttacggg cagggcggtg gtaaagcccc gcgtcgagtc aggcctcgtc aggtccgacc
      781 cggtaggtga cgtgctcaag atcgtcgtgg tgaacaggta ccgcgaccag gcgcccgccg
      841 tcgcctttgc gagaggcttc ggccttgcct cgggtgccat cgcatcctcc gttgcgcacg
      901 attcgcacaa catagtggcc gtgggtgtcc aggacgaaga catctgcgcc gcggtggacg
      961 cggtcatggg aggcgggggc ggcatagccc tggccgcggg cggccgtgtc gaatgccttg
     1021 ggctgccggt cgcaggcctc atgtcggacc tcgaagcaca ggaggtcgca ggccgcttcg
     1081 cgcgactcaa tgacatggcg ggcagtctcg ggtcgcctct tgcgagcccc ttcatcacgc
     1141 tgtcgttcat ggcgctgctc gtcatcccgt cgctcaagct gagcgacagg ggtctcttcg
     1201 actccgagag gttcgcgttc acctccctgt gggatgcctg acgattgtcg tgaaggggtt
     1261 catcccgccg ctgagtgcca tcgctcgagc gtgtcctggt cgaggggcct gaagatcctc
     1321 gatggagggt ttgccccaaa ggcaggtcgt cgacgggctg aagggcggtc gcctgtgccc
     1381 tgggtgcgac atgcaaatcc gcctgctctt tcccgcctcg gcggttgggg gccctccccc
     1441 ggcgacggtc gtcttcagga gagtgcttgt ttttccgata cagcatacca agaatgaaca
     1501 acggggggca ttggtccgca tgagacgtcc cggcagacga gcctcgtccg ggggtaaggg
     1561 cgcgagacgc ctccacgggg atgggttccg tgaccgaggg cccgctctgt ccgttttccg
     1621 ggcgacaccg catggagagt atccgtgcgg cgcgcggtgc aggatggtgg gatcctctct
     1681 cgagagggag aaccctttcc caggaggggg gcgcaccctt gggccgcatg gccggccgcc
     1741 tgttccaggg gaaaggggcg gggaagagga taagcacctg atggggaaac gccgcccgga
     1801 tgacggggtc tgccgtgaga gcgcatggaa acatggcgtc ttgtgcgagg catgatgcat
     1861 gggggcggag aggccggcat ggatgacctc gtggccaagg atgagcggat ggtaacccgg
     1921 gatacgctga aagacgaggg tgtccccggc gtgaaggcgg tgtgctgcga gactccggac
     1981 aggccgttca gtacgtccgc cgacggcgaa gagggagacc cgatcgatct cgagcaggcg
     2041 ctttccgagt tcgatggcga cagggacttt ctccaggggc tgctgcgggc gttcctcgag
     2101 aacgtcgggc ggcagatccc ggccttgagg cgggcgatcg gaacggccga cgcgcaggag
     2161 gtgaggtccc tcgcccacgc catcaagggc ggcgccggga atctcggggc ccagagactc
     2221 gccgagtgcg catgggcgat ggagcggatg gcgggagagg gtggtctcgc acgggcccac
     2281 gaggccctgg agaggctcga ggcccaacac gagaggctca aacggtatgt ctccgaacgg
     2341 ctgtgaagaa ctgcacgtcc cggtacgcgt gccacgtgca ctcaggaaat cgccacgggg
     2401 tgtccttgcg cctgcgtgga gggccggcgg tcttgcggcc gtatcgtgca aggagggttc
     2461 gaggtcaggg gaatgagggt cctcgttgtc gacgacgagc tggtgagcag ggagaagatg
     2521 aggcgcatca tggccggcct cttcgggggg tgcgacgagg cgtccaccgg gcaggccgcc
     2581 ctcgaggcag tgagcgcggc catgtccgag aacaggccct acgagctcat cacgctcgac
     2641 atctcgatgc cctccatgga cggcatcgac gtgctcaaga ggatcaggga gtcggaacgg
     2701 gccgaaaacg tcccgaagga tgcacgggcg aagatcttca tggtcacggc agcgtccgag
     2761 agggagacca tcatcacctg tatcaaggaa gggtgcaacg actacatcac gaagcccttc
     2821 agcagcgaca gggtgctcaa gaagctcagg gacaacggga tcctcccgcc cgagccggac
     2881 ggcgctccgg caggggccca ggcatccgcc gcgaagaact cgctcgacgg cgtgatcgag
     2941 cggttcaaca ggggcgagat ggaactgccg cccatgcatc tcatccagcg caagttctac
     3001 gagctcacgg cctcgggcgc cacgctggcg gcgatcgcgg atctcctcga aaaggacccg
     3061 gccatctcgt cgaagctcat aaggatatcg aattcatcgt tctacagggg gtacaacgag
     3121 aacgtcaccg tcgagcaggc agtcaaccgc ctcggcctca tcgccacgaa acagaccgtg
     3181 gacgcggtgt gcagcagggc tttgtacgac gacgtgcacc cgaactacgt gcaggtggtg
     3241 gaaaagctct gggtgcactc gctggcctgt gcgtacgcct gccagctcat cgccgagttg
     3301 agggggatca ggctcttgga ggatcccttc accatgggcc tgttccacga catcggcaag
     3361 ctcatgctgc tgagggtcat cggcgagctc caaaggagcg gcaaggatgt cgataccgac
     3421 accgacatgc tcatgtcggc cgtggagacg caccacggca aggccggcgc ggtcctcctg
     3481 aagaagtggc tcttcccggc cgtgttcgtc caggtggccg agctccacga tgacctcggc
     3541 aaggtgctca cccccggcag ggaactgctc gtcgtgcagc tggccaacat cgcggtcaag
     3601 tccctggggt acggcacccc gaacgcacag ggcgtgcagc ccggggcctc cgagcaggcg
     3661 aagtccttgg gtgtcgaccc gtccgtgatg gatctcgtcg ggacccaggt caaaaagtac
     3721 atggacgaat tcgtggaata cctctcgtga gcaacggcga gcgctcgtgg cccggcttgg
     3781 tctcctgcag ccgcgacggg tcc
//
LOCUS       DAOSCR010000224         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15278_length_3794_cov_3.484622, whole genome shotgun sequence.
ACCESSION   DAOSCR010000224 DAOSCR010000000
VERSION     DAOSCR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15278_length_3794_cov_3.484622"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLT69_06560"
     CDS             <1..782
                     /locus_tag="PLT69_06560"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOJ14133.1"
                     /translation="FISPGSNASVFALEYKPRAYNISNVLMRTPEIYHKEVLEMPEAP
                     RVDPGQPLSIHDMRRAASDEIRKLLVYDTYRKDCFVTHYLKTPASVEKILNENRIENS
                     LSAQAPFELIGIDEKDGSLVVSFGMEGDGFSIRKTYGYDPAGCVTLSHEVYPQDHDSW
                     LAIEWNIFTLSGERPMVDGREMEDDRGVYRSRLVTLRDTVKGLEVVVSSPDYWVCCVV
                     PIECVSQSEEGFEKTFQGHSIYFMQQCSGKVPDVSLTIRSL"
     gene            818..1525
                     /locus_tag="PLT69_06565"
     CDS             818..1525
                     /locus_tag="PLT69_06565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOJ14134.1"
                     /translation="MPRFFCRITRFPGAVIDDTPSVAHLRKALRKRVGDELDVRVGEQ
                     GYLARITGLGPTEATLELIEEVTLHDRTHKVIHLALCLFDLREWEWALRSVVELGVSH
                     VHPLVSKRSQVRSVTHARKERWRLIALEAVKQCGRRSVPVLHDPQGLKDFAEQVSRDW
                     EWRFVAVGGAHDPLAPALGREGGIVIGPEGGLTDDEMETLKRAGFRAAGMGDTTLRSV
                     TAAVAAMAILGQQVQSG"
     gene            complement(1580..>3794)
                     /locus_tag="PLT69_06570"
     CDS             complement(1580..>3794)
                     /locus_tag="PLT69_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14135.1"
                     /translation="EKRNAYLESARSYENEGKLAEAAIQYQNALQIAPDDAATLVSLG
                     EVQLKLNRPQEAYASFAKAAKTDPKNVKSREYMASMLLLSKRYDLAQKEAEAIIAIDP
                     KHTLAREIRAQALFMSGNRDEGVRTMEELLKTEKPTEEMYLNTIQMYMAVGRTDDALT
                     LVDRGAALFPSSTKLRFMASDIHSMRKDAAKARRWAEEAYRVSNGSVSAGVALAMFYA
                     RNNMDELYKNQLKELMARHPKNPEPYLLEATVYHTKGDLDGALASARKARSIEDTAQV
                     RVLISQLLLEKKQPGEAKAILKESIQKDGQDFMARIMLAQILLDEKNPTEALEVLDEP
                     IKKIPTSPDLAATASQAYLLKGDVQKARQLVEKALAASPQNVALTRMMAKILYVQGDS
                     KAALDQTRTLVGKQVKTPDILYIGALSALREGRLDEAKSYVDELDKHAPETWPTLHAH
                     ILWHLQRKEAKAAFPYAERAVEKEPGNEEALALYASLAPKTIGLAKTVEKVRSLCSKK
                     ETSTCHMVLALLVEQAGNKDEALAEIKKAIALDPGRTSSYHALAQYYTRNNMTRKAID
                     EYEAILNKNPNDLAAASMIALLQQDAGDLEAAKKAYRYVLDRDPKHGFAANNLAWILA
                     QKGSKKDLDEALRLAQIAKDRYPDDPRVADTLGFVYLGKGMAEAALGQFQMALDKMPE
                     EPTLLYHKAQALAGMGRTADAVSSLKKALSSAKPFPEKREAQELLARLSQKAGTK"
BASE COUNT          587 a         1178 c         1227 g          802 t
ORIGIN      
        1 gtttcatatc cccggggtcg aacgcctccg tgttcgccct ggagtacaag ccgagggcgt
       61 acaacatctc gaacgtgctc atgcgcaccc ccgagatcta ccacaaggaa gtcctggaga
      121 tgcccgaggc tccccgtgtg gaccccgggc agcccctgtc gatccatgac atgcgaaggg
      181 ccgcgagcga cgagatccgc aagctcctcg tctacgatac ctaccggaaa gactgcttcg
      241 tgacccacta cctcaagacc cccgcatcgg tggagaagat cctcaacgag aaccggatag
      301 agaactcctt gagcgcccag gcccccttcg agctcatcgg gatcgacgag aaagacgggt
      361 ccctggtcgt gtccttcggc atggaagggg atggcttctc gatcaggaag acctacgggt
      421 acgaccctgc cggctgcgtc acgctctcgc acgaggtgta cccgcaagac cacgattcgt
      481 ggctggccat cgagtggaac atcttcaccc tgagcggcga gagacccatg gtggacggcc
      541 gcgagatgga ggacgacagg ggcgtgtaca ggtcccgcct ggtgaccctg agggacaccg
      601 tgaagggctt ggaggtcgtg gtttcctcgc ccgattactg ggtgtgctgc gtggtgccca
      661 tcgagtgcgt ctcgcagagc gaggagggat tcgagaagac cttccagggg cacagcatct
      721 acttcatgca gcagtgttca ggcaaggtcc cggatgtgtc cctcaccatc cgctccctct
      781 aatgggatag caccggatga tgcacggttg attgtccatg ccgaggttct tctgcaggat
      841 aaccaggttt cccggcgccg tcatcgacga caccccctcg gtcgcccacc tgaggaaggc
      901 cctccggaag agggtgggcg acgagctcga cgtgagggtc ggtgaacagg ggtacctggc
      961 gaggataaca ggcctcggcc ccacagaggc gaccctcgag ctcatcgaag aggtcaccct
     1021 ccacgaccga acccacaagg tgatccacct cgccttgtgc ctgttcgacc tcagggagtg
     1081 ggagtgggcg ctccgctcgg tcgtggagct cggtgtctcg cacgtccatc ccctggtttc
     1141 caagaggtcc caggtcaggt ccgtgaccca tgccaggaaa gagcggtggc gtttaatcgc
     1201 gctcgaggcg gtgaagcagt gtggcaggag gagcgtgccg gtcctgcacg acccgcaagg
     1261 gctgaaagac tttgccgaac aggtgtcgag ggactgggaa tggaggttcg tggcggtggg
     1321 gggcgcccat gaccctctcg cccccgccct cggccgtgag gggggtatcg tgatcgggcc
     1381 cgagggaggg ctgaccgacg acgagatgga gacgctcaag cgggcggggt tcagggctgc
     1441 gggcatgggt gacaccacgc tccgctccgt cacggcggct gtcgcggcga tggccatcct
     1501 cggccagcag gtgcaatcag gatgatgcag tgcgccggcc cctgtgcagc tcagggttct
     1561 ccccggccgc tcgatgggtt cacttggtcc ctgccttttg agagagtctt gccaggagtt
     1621 cctgcgcctc tctcttctcc gggaagggct tcgcgctcga cagggccttc ttgagcgagc
     1681 tcacggcatc ggccgtccgg cccatcccgg caagggcctg ggccttgtgg tacaggagcg
     1741 tgggctcctc cggcatcttg tccagcgcca tctggaactg accgagggcc gcctcggcca
     1801 tgcccttgcc gaggtacacg aacccgaggg tgtcggccac cctcgggtcg tccgggtacc
     1861 tgtccttcgc gatctgggcg agcctcagcg cctcgtcgag atcctttttc gagcccttct
     1921 gggccaggat ccacgccagg ttgttggcgg cgaacccatg cttcgggtcg cgatcgagca
     1981 cgtacctgta cgcctttttc gcagcctcca gatcgcctgc gtcctgctgg agcagggcga
     2041 tcatggacgc agccgcgagg tcgttggggt tcttgttgag gatggcctcg tactcgtcga
     2101 tggccttccg ggtcatgttg ttgcgggtat agtactgtgc gagcgcatgg taggaggagg
     2161 tgcggccggg gtcgagcgcg atcgccttct tgatctcggc aagggcctcg tccttgttgc
     2221 ccgcctgctc caccaggagg gcgagcacca tgtggcatgt cgacgtctct tttttcgagc
     2281 agagggatcg taccttttcc acggtctttg caaggcctat ggtcttcggg gccagggatg
     2341 cgtacagggc gagcgcctct tcgttgcccg gttccttctc cacggcccgc tcggcatagg
     2401 ggaaggccgc cttcgcctcc ttcctctgga ggtgccagag gatatgcgcg tgcaaggtcg
     2461 gccaggtctc cggtgcatgc ttgtccagct cgtccacgta ggacttcgcc tcatcgagac
     2521 ggccctcgcg cagggccgac aaggcgccta tgtagaggat gtcgggtgtc ttcacctgct
     2581 ttcccacgag ggtgcgggtc tggtcgagcg cggccttgga atccccctgc acgtagagga
     2641 tcttcgccat catcctcgtg agggccacgt tctgtggtga cgctgccagg gctttctcga
     2701 cgagctggcg cgccttctgg acgtctcctt tgagcaggta ggcctgcgat gccgtcgctg
     2761 cgaggtccgg gctcgtcggg atcttcttga tcggctcatc gaggacctcg agtgcctccg
     2821 tcgggttctt ttcatccagg aggatctggg cgagcatgat cctggccatg aagtcctggc
     2881 cgtccttctg gatcgactcc ttgaggatcg ccttcgcctc gccgggttgc ttcttctcga
     2941 ggagcagctg ggagatgagg accctcacct gggcggtgtc ctcgatggac cgtgccttgc
     3001 gggccgaggc gagggcgccg tccaggtcgc ccttcgtgtg gtacacggtg gcctcgagca
     3061 ggtacggctc ggggttcttg gggtgcctcg ccatgagctc cttgagctgg ttcttgtaga
     3121 gctcgtccat gttgtttctc gcatagaaca tggcgagcgc cacccccgcc gaaacgctcc
     3181 cgttggatac ccggtaggcc tcctcggccc atcttctggc cttggccgcg tccttcctca
     3241 tcgagtggat gtcgctcgcc atgaacctga gcttcgtgga ggaggggaac agcgccgccc
     3301 ccctgtccac gagcgtgagc gcgtcgtccg tcctcccgac cgccatgtac atctggatgg
     3361 tgttgaggta catctcctcg gtcggtttct cggtcttcaa gagctcttcc atcgtccgga
     3421 cgccctcgtc ccggttgccg ctcatgaaca acgcctgtgc ccttatctct cgtgcgagcg
     3481 tgtgcttggg gtctatggcg atgatggcct cggcctcctt ctgagccaga tcgtacctct
     3541 tggacaacag gagcatcgac gccatgtatt ccctggactt gacgttcttg ggatcggtct
     3601 tagcagcctt cgcaaacgac gcgtatgcct cctggggacg gttgagcttg agctgcacct
     3661 ctcccaggct cacgagggtg gcggcgtcgt ctggggctat ctggagggcg ttctggtact
     3721 ggatggccgc ttccgccagt ttcccttcgt tctcgtagga ccttgcgctt tcgaggtatg
     3781 cgtttctctt ctct
//
LOCUS       DAOSCR010000225         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15284_length_3793_cov_3.232477, whole genome shotgun sequence.
ACCESSION   DAOSCR010000225 DAOSCR010000000
VERSION     DAOSCR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15284_length_3793_cov_3.232477"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /gene="lon"
                     /locus_tag="PLT69_06575"
     CDS             complement(<1..1805)
                     /gene="lon"
                     /locus_tag="PLT69_06575"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ14136.1"
                     /translation="MKIVASEPVMPLLALTDIVVFPHTIIPLLIENPRAAEAVCAAYE
                     AGREILVACRRHGTAEGSASGPVHSVGVSCRVLQLLRLADGQLKIFIEGRARKLIKTV
                     HTLEDNLYATTEPFIDFIVDEREAEALRRLVLEAFKEYAERLSIDIAQDLMEKVVNQE
                     DAGVVSDIVASYSSMKTDTKQEILEESHIESRLERIYESLRSEIEIARIKEEVREKTR
                     RKTTSSQRPSSAAQMYTQGGSQEGEDEAAMLERQLMAKSMPVHAREKALQEIRRLRMM
                     PPMSAEATVVRNYVDWFLALPWDETTEDKKDIAFAQKVLDEDHYGLGKVKERILEYLA
                     VQQMVGTTRGAILCLVGPPGVGKTSLGKSIARATGRKFVRVSLGGVRDEAEIKGHRRT
                     YIGALPGKIIQGIRRAQSSNPVFLLDEVDKMSMDFRGDPSSALLEALDPEQNSAFNDH
                     YLDCDYDVSRVMFITTANTTHTIPAPLLDRMELIHLEGYTSYDKLKIATNHIVPKQLR
                     LHGIEDRGIRFSQSAIRTVIHEYTREAGVRNMEKEISSICRKLVRSIVSGAEPPRRVL
                     TRRDVHEYLGVPRFRRARLSSGDAVGVVNGLAWTEA"
     gene            complement(1837..3090)
                     /gene="clpX"
                     /locus_tag="PLT69_06580"
     CDS             complement(1837..3090)
                     /gene="clpX"
                     /locus_tag="PLT69_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOJ14137.1"
                     /translation="MPRINDHYRPGLRCSFCGKSQEEVRKLIAGPDVYICDECIGLCN
                     EILAEEEGEYRHFGAASTIPKPSEIKEVLDEYVIGQEMTKRILSVAVHNHYKRIESNT
                     SIGGVEISKSNILLIGPTGSGKTLLAQTLAKILDVPFTIADATTLTEAGYVGEDVENI
                     IVNLLQNADYDVERTQKGIVYIDEIDKIASKGDNPSITRDVSGEGVQQALLKMVEGTM
                     ASIPPKGGRKHPQQEFIQIDTTNILFICGGAFVGLDKIISQRLDKKTIGFVADITGKE
                     ERDVGEILKHVQPEDLIKFGLIPEFVGRMPIVSTLHELDENALIAILTEPKNALVKQY
                     QALLKLEGVDLTFTKDALKAVAQEAMKRKSGARGLRSIMEGIMLDIMYDIPDMEGVKE
                     CIISQDVVLKGKPPIIVYERKSSQA"
     gene            complement(3077..3700)
                     /gene="clpP"
                     /locus_tag="PLT69_06585"
     CDS             complement(3077..3700)
                     /gene="clpP"
                     /locus_tag="PLT69_06585"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOJ14138.1"
                     /translation="MTLVPIVVEQTGRGERSYDIYSRLLKDRIVMLGTEIDDDVANLL
                     VAQFLFLESEDPDKDISFYINSPGGSVTAGLAVYDTMQYIKPKVSTICIGQAASMAAL
                     LLAAGEPGMRYALPHARILIHQPLGGAQGQVTDVNIQVNEMLRLKDALTRILSKHTGQ
                     PIDKVAIDTERDYFMAVTDAQKYGIIDHVITKRVTVEKEGEKADAQD"
BASE COUNT          581 a         1211 c         1161 g          840 t
ORIGIN      
        1 gcctccgtcc aggcgagccc gttcacgacc cccacggcgt cccccgagga gagccgcgcc
       61 ctccggaacc gggggacccc caggtactcg tgcacatccc tcctggtgag cacccgcctg
      121 ggcggctcgg cgcccgacac gatcgacctg acgagcttgc ggcagatgga ggagatctcc
      181 ttctccatgt tgcgcacgcc ggcctcgcgg gtgtactcgt ggatgaccgt cctgatcgcc
      241 gactgcgaga accttatccc cctgtcctcg atcccgtgca gcctcagctg cttggggacg
      301 atgtggttgg tcgcgatctt gagcttgtcg taggatgtgt agccctcgag gtggatgagt
      361 tccatcctgt ccaacagcgg tgcagggatg gtgtgcgtgg tgttggccgt ggtgatgaac
      421 atcacgcggg acacgtcgta atcgcagtcg aggtagtggt cgttgaatgc gctgttctgc
      481 tccgggtcga gggcctcgag cagggccgag gacgggtcgc ccctgaagtc catgctcatc
      541 ttgtccactt cgtcgaggag gaacacgggg ttcgaggatt gggcgcgcct gatgccctgg
      601 atgatctttc ccgggagcgc cccgatgtag gtgcggcggt ggcccttgat ctccgcctcg
      661 tccctgaccc cacccaggga gacccggacg aacttcctcc cggtcgccct cgcgatggac
      721 ttgccgaggg atgtcttccc gacgccgggg ggacccacga ggcagaggat ggcgcccctg
      781 gtggtcccca ccatctgctg caccgcgaga tactcgagga tccgttcctt gaccttgccg
      841 agcccgtagt ggtcctcgtc gaggaccttc tgcgcgaacg cgatgtcctt cttgtcctcc
      901 gtggtctcgt cccagggcag cgccaggaac cagtcgacgt agttcctcac caccgtggcc
      961 tcggcgctca tggggggcat catcctgagg cgccttatct cctggagcgc cttctccctg
     1021 gcgtgcacgg gcatgctctt ggccatgagc tggcgctcga gcatggcggc ctcgtcctcc
     1081 ccctcctgtg acccgccctg ggtgtacatc tgggccgcgg acgaggggcg ctgggaggag
     1141 gtcgtcttgc gcctggtctt ctccctcacc tcctccttga tccgcgcgat ctcgatctcc
     1201 gacctcaagg actcgtagat cctctccagc ctcgactcta tgtggctctc ctcgaggatc
     1261 tcctgcttgg tgtccgtctt catggaggaa taagacgcca cgatgtccga gacgacgccg
     1321 gcgtcttcct ggttgaccac cttctccatg aggtcctggg cgatgtctat ggagaggcgc
     1381 tccgcgtact ccttgaaggc ctcgaggacg agcctccgga gcgcctcggc ctcccgctcg
     1441 tcaacgatga agtctatgaa gggctcggtg gtggcgtaga ggttgtcctc caaggtgtgc
     1501 accgtcttga tcagcttcct cgccctgccc tcgatgaaga tcttgagctg cccgtccgcg
     1561 agtctcagga gctggagcac cctgcaggac acgccgacgg agtggacagg cccggatgcc
     1621 gagccctcgg ccgtaccgtg cctccggcac gcgaccagga tctccctgcc cgcctcgtaa
     1681 gcggcgcaca cggcctcggc ggccctcggg ttctcgatga gcaggggtat gatggtgtga
     1741 gggaagacga cgatgtccgt caacgccaac agcggcatga cgggctctga ggccacgatc
     1801 ttcatggcag ggtgctcttc gcctccaggt tcggtatcag gcctgggagg acttgcgctc
     1861 gtagacgatg atcggcggct tgcccttcag caccacgtcc tggctgatga tgcactcctt
     1921 gacgccttcc atgtcgggaa tgtcgtacat gatgtcgagc atgatgcctt ccatgatgga
     1981 cctcagcccc ctcgcccccg acttgcgctt catggcctcc tgggccaccg ccttgagcgc
     2041 gtccttggtg aaggtgaggt ctacgccctc gagcttgagc aaggcctgat actgcttcac
     2101 cagggcgttc ttcggctcgg tgaggatcgc tatgagcgcg ttctcgtcga gctcgtggag
     2161 ggtggacacg atgggcatgc ggccgacgaa ctcgggtatg agcccgaact ttatgaggtc
     2221 ctcgggctgc acgtgcttga ggatctcccc cacgtcgcgc tcttccttgc ccgtgatgtc
     2281 ggccacgaaa cctatggtct tcttgtcgag gcgctgggag atgatcttgt cgagccccac
     2341 aaaggccccg ccgcagatga acaggatgtt ggtggtgtcg atctggatga attcctgctg
     2401 cgggtgcttc ctcccaccct tgggggggat ggatgccatg gtgccttcca ccatcttgag
     2461 cagcgcctgc tggacgccct cgcccgagac gtccctcgtg atggacgggt tgtccccctt
     2521 ggatgcgatc ttgtcgatct cgtcgatgta gacgatgccc ttctgcgtgc gctcgacgtc
     2581 gtaatccgcg ttctggagca ggttcacgat gatgttctcg acgtcctcgc ccacgtatcc
     2641 cgcctcggtc aacgtggtgg cgtccgcgat ggtgaagggc acgtcgagta tcttcgcgag
     2701 ggtctgcgcc aggagggtct tgcccgaccc ggtggggccg atgagcagga tgttgctctt
     2761 gcttatctcg acgcccccga tgctcgtgtt cgactcgatc ctcttgtagt ggttgtggac
     2821 ggcgacggag agtatgcgct tggtcatctc ctggccgatc acgtattcat cgagcacttc
     2881 cttgatctcg gagggtttcg ggatggtcga ggccgccccg aagtgcctgt attcaccttc
     2941 ttcctcggcc agtatctcgt tgcagagacc gatgcactcg tcgcagatgt acacgtcagg
     3001 accggctatg agcttgcgga cctcctcctg cgacttcccg cagaaagaac accgaaggcc
     3061 gggcctgtag tgatcgttaa tcctgggcat ccgccttctc cccttctttc tccaccgtca
     3121 ccctcttcgt gatcacgtga tcgattatac catacttctg ggcgtcggtc acggccatga
     3181 agtagtcccg ctccgtgtct atggctacct tatctatagg ctggccagtg tgcttggaca
     3241 agatcctcgt gagggcgtcc ttgagcctga gcatctcgtt gacctggatg ttcacgtcgg
     3301 tgacctggcc ctgggcgcct cccaacggct gatggatcag gatccgggcg tgtgggagcg
     3361 catagcgcat gcccggctcg cctgcggcaa ggagcagggc cgccatggac gccgcctgcc
     3421 cgatgcatat cgtgctgacc ttgggcttta tgtactgcat ggtgtcgtac accgcgagcc
     3481 cggcggtcac cgagccgccg ggggagttga tgtagaacga gatatccttg tccgggtctt
     3541 ccgactcgag gaacagaaac tgcgccacga gcaggttcgc cacgtcatcg tcgatctccg
     3601 tgccaagcat gacgatcctg tccttgagta gcctcgagta gatgtcgtag gagcgttcgc
     3661 ccctcccggt ctgctcgacc actatgggaa ccagcgtcat cagctcttcc cctccctggc
     3721 cttgtccact tccacgacct tcgaatgctc tatgatgtag tcgtacatct ttctctcgag
     3781 gatcccgtag cgc
//
LOCUS       DAOSCR010000226         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15355_length_3781_cov_3.625872, whole genome shotgun sequence.
ACCESSION   DAOSCR010000226 DAOSCR010000000
VERSION     DAOSCR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15355_length_3781_cov_3.625872"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLT69_06590"
     CDS             <1..484
                     /locus_tag="PLT69_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14139.1"
                     /translation="AYAPVRIYGKGSVFIDERCSVFYSVFRGLTIMTLTPEARVSIGK
                     GCDLGGLTIRCCKEVSLGDKVMTAVSLVQDTPIVHDGATPARHPGANWCKPVSIGDKV
                     WLAASSAVLQGASIGEESVLSVGSCIMGQAAPGRSLLVGNPVRRPVLIERILGLRKNA
                     "
     gene            481..1059
                     /locus_tag="PLT69_06595"
     CDS             481..1059
                     /locus_tag="PLT69_06595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ14140.1"
                     /translation="MKELVAVLKGQVFRWRCALFYPNVTIGKGLRIFGRLLINARGRV
                     KIGDNCLVREIPGSEHKCACIDVISPEAYVTIGDNAVLCAARITARYGITIGDNVIVE
                     DAGVVDTDFHDIDASRSTPTDENHDRCRITIGSNVSIGALSFITKGVTIGDGAVVAPA
                     SVVTKPVKPGSFVYGNPAVVRPMPHETEAPKT"
     gene            1056..1907
                     /locus_tag="PLT69_06600"
     CDS             1056..1907
                     /locus_tag="PLT69_06600"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOJ14141.1"
                     /translation="MTTKGALTRGNIVSLFLVVGVAVAAYWSTFVDLYHMWSSNEDYS
                     HGFLVIPLAVYLAWRKRHEMLDKATDSSSMGWGLAMVWVILYVAGSMGHIATVKNVSM
                     VVLPIASSAIVLGPAAARCMMVPSFFLLFMFPIPSEIYTRVTNPLLLISTTASYHILG
                     SLSIPVLQDGNLLSLPDYTMEVVLACSGIRSLITIMALAYLMAALMLRGVLQWVMLLL
                     ASVPTAIAGNILRITLTAVLAYHVSPKAAEGFSHTLVGVATFIVAFVLLSTCMVLIRW
                     MFEPKKR"
     gene            1880..2536
                     /locus_tag="PLT69_06605"
     CDS             1880..2536
                     /locus_tag="PLT69_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HOJ14142.1"
                     /translation="MDVRTQKTLALAILILAVSWSAVYLDRPQGQPAASSYELPEVIG
                     QWRGAEVDFDREKLEAWLGTDRMVFREYRNATSGTTVTVYVAYYPALESSDMAHEPEV
                     CYPGQGWSIVSNTLSVLDLPVAAVATKRMVIEKGSRREVVHSWWQTRDRSIARNWSYR
                     LYQTLRCVMGRETSSLWVRVSCEYEQGITDPLKGDSAILAFCTELYPLLLPYFRTGPG
                     "
     gene            2533..3675
                     /locus_tag="PLT69_06610"
     CDS             2533..3675
                     /locus_tag="PLT69_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ14143.1"
                     /translation="MTIAFFASLFVLLYIYAGYPLVMFALSRLRQRGVAKGAFFPRVT
                     IIISAYNEERHIGATIANKLALDYPKDRLEIIVVSDGSDDSTDDIVRSFNQRGVRLIR
                     QEPRQGKTSAVNLAVSKAGGEILVFSDANSIYAPDALEHLVRCFADPEVGYVTGKMVY
                     RTNEGSMVGQGCSAYMRYENLLRAWETLVGSVVGVDGGIDAVRRELYMPMRPDQLPDF
                     VLPLEVVARGYRVVYEPRALLVEDALKDAMDEYRMRVRVTLRALWALWDMRNLLNPLR
                     HGVFSMQLLSHKVLRYLAIIFMVTALFTNMFLAAKSLVFRFLLVAQAVFYGAAAAGWA
                     YGRKNASLPNLLYIPYYFCLVNIAAAHAILKFLRGEKQVTWTPRKG"
BASE COUNT          742 a         1090 c         1148 g          801 t
ORIGIN      
        1 ggcgtacgcc cctgtgagga tatacggcaa gggctccgtc ttcatcgatg agcgatgctc
       61 ggtgttctac agcgtgtttc gggggctcac catcatgacg ctgacccctg aggcccgagt
      121 gagcatcggc aaggggtgcg acctcggggg gctcaccatc aggtgttgca aggaggtgag
      181 cctgggtgac aaggtcatga cggcagtgag cctcgtgcag gacacgccca tcgttcacga
      241 tggtgcaacc ccggcacgcc acccgggtgc gaactggtgc aagccagtct ctattggcga
      301 taaggtctgg ctggccgctt ccagcgcggt tttgcaaggc gcatccatag gagaggaaag
      361 cgtcctgtcc gtgggctcgt gcatcatggg tcaagcagct ccgggtcgct ccttgctggt
      421 gggaaacccg gtgaggaggc ctgttttgat cgaaagaatc ctcggcttga ggaagaacgc
      481 atgaaagaac ttgtcgcagt cctcaagggg caggtcttca ggtggcggtg cgccctcttc
      541 taccccaacg taacgatcgg caagggcttg cgtatctttg ggaggctctt gatcaacgcg
      601 agaggaaggg tgaagatcgg cgataactgc ctggtacgcg aaatacccgg ctcggagcac
      661 aagtgcgcct gcatagacgt gatatcccca gaggcctatg tgaccattgg agacaatgcc
      721 gtgctgtgcg cggccaggat cacggcccgg tacggcatca cgatcgggga caacgtgatc
      781 gtagaggatg ctggcgtcgt cgatactgac tttcacgaca tcgatgcctc gagatctacc
      841 cctacggacg agaaccacga ccgttgccgg atcaccatcg gcagcaacgt gagcataggg
      901 gcgctcagtt tcataacaaa gggcgtcacg atcggggacg gcgctgttgt cgcgccggca
      961 tctgtcgtga cgaagccggt caagcccggc agcttcgtgt atggaaaccc ggcagtagtt
     1021 cgcccgatgc cacacgagac agaggctcca aagacatgac gacgaaaggc gcccttacca
     1081 ggggcaacat cgtgtctctg ttcctcgtcg ttggggttgc cgtggcggca tattggtcga
     1141 cctttgtcga cctttaccac atgtggtcaa gcaacgagga ctacagccac ggctttctcg
     1201 tcatccctct ggcggtctac cttgcctgga ggaagcgtca cgagatgctc gacaaggcga
     1261 cggacagctc gtccatgggg tggggcctgg cgatggtctg ggtgatcctc tatgtggcgg
     1321 gttccatggg ccatatcgcc acggtgaaaa acgtctcgat ggttgtactg cccatcgcgt
     1381 cgagtgccat cgtgctcggc ccggcagcag cccgatgcat gatggtccca tcgttcttcc
     1441 tgctcttcat gttccccatt ccatccgaaa tctacacgag ggtcacgaac cccctcctgc
     1501 tgatctcgac cacggcttca taccacatcc tgggctcgct gagcatcccg gttctgcagg
     1561 acgggaacct gctcagcctg ccggactaca ccatggaggt ggtcctagcc tgctcgggta
     1621 tacgatccct catcaccatc atggcgctgg cctatctcat ggccgcgctc atgctgagag
     1681 gggttctgca gtgggtcatg cttctcttgg catccgtccc gaccgcgata gccggaaaca
     1741 tcctgaggat cacgctcacc gcggtgctcg cctatcacgt gtcgccaaag gcggcggaag
     1801 gcttctcgca cacccttgtg ggggtggcga ccttcatcgt ggcattcgtt ctcctctcaa
     1861 cgtgcatggt cttgatacga tggatgttcg aacccaaaaa acgctagcgc tcgcgatcct
     1921 gatcctagcc gtttcctggt cggctgtata tctcgacagg cctcaggggc aacctgcagc
     1981 ctcatcctat gaactgccgg aggtcatcgg gcagtggaga ggcgccgagg tcgacttcga
     2041 cagggagaag cttgaggctt ggctcggcac cgacaggatg gtgttcaggg aataccgcaa
     2101 cgccacaagc ggtaccaccg tgacggtcta tgtagcctac tacccggcgc tcgagagttc
     2161 cgacatggcc cacgagcccg aggtgtgcta cccggggcag gggtggtcca tcgtctccaa
     2221 caccttgtcc gttctggatc tgcctgtggc tgccgtggcc acgaagcgca tggtcatcga
     2281 gaaggggtcc aggagagagg tggttcactc ctggtggcag acccgtgatc gttccattgc
     2341 aaggaattgg tcgtatcgcc tctaccagac actcagatgt gtgatgggaa gagaaacgtc
     2401 ctctctgtgg gtgagggtgt cctgcgaata cgagcagggg ataaccgacc ccttgaaggg
     2461 ggacagcgcc attttggcat tctgcaccga gctgtatcca ctccttttac cgtatttccg
     2521 gacaggcccc ggatgacgat cgcctttttc gcaagcctgt tcgttctcct ctacatctat
     2581 gcgggctacc cgctcgtcat gtttgccctg tcgcgcctga gacaaagggg tgttgcgaag
     2641 ggggctttct ttcccagggt cacgatcatc atctcagcgt acaacgagga acggcatata
     2701 ggcgcaacca tcgccaacaa gctcgctctc gactacccga aagacaggct cgagatcatc
     2761 gtggtgtcgg acgggtctga cgactcaaca gacgacatcg ttcgttcctt caaccaaagg
     2821 ggcgtgaggc tcatccgcca ggagccaagg cagggaaaga cctcggccgt caacctggcc
     2881 gtgagcaagg ccggcggaga gatcctcgtg ttctctgatg caaactcgat ctatgcgccc
     2941 gatgcactgg aacacctcgt gcgatgtttc gcagaccctg aggtgggcta cgttacagga
     3001 aagatggtgt acagaacgaa cgaaggctcc atggtggggc aggggtgttc ggcgtacatg
     3061 aggtacgaga acctcctgag ggcatgggag accttggtgg gatccgtcgt cggcgtggac
     3121 ggcggcatcg atgcggtacg aagggagctc tacatgccca tgaggccgga ccagctgcct
     3181 gacttcgtgc tccccctgga ggtggtcgcc cgtggatacc gggtggtata cgaaccacgc
     3241 gccctgctcg ttgaagacgc actcaaggac gcgatggacg agtaccgcat gagggtgcgg
     3301 gtcacactgc gcgcgctgtg ggccctgtgg gacatgcgta acctcctgaa ccccctgagg
     3361 cacggtgttt tctctatgca gctgttgtcg cacaaggtgc tcaggtacct ggccatcatc
     3421 ttcatggtga ccgcactttt caccaatatg ttccttgcgg ccaagtctct cgtcttcagg
     3481 tttcttctcg ttgcccaagc cgtgttctac ggtgcggccg cggctggctg ggcgtatggt
     3541 agaaagaacg catcgttgcc caaccttctt tacatccctt actacttctg cctggtcaac
     3601 atcgctgcag cacatgccat cctgaagttc ctgcgcggcg aaaagcaggt aacgtggacg
     3661 ccacggaagg gatgaaaccg gccacataca gtcttgggta cgttttccct cttattactg
     3721 gaagaagcga ggtgaaggtg gcaccctgca ccttgagcga tcggttgggc ctctgctgtt
     3781 t
//
LOCUS       DAOSCR010000227         3733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15646_length_3733_cov_3.293094, whole genome shotgun sequence.
ACCESSION   DAOSCR010000227 DAOSCR010000000
VERSION     DAOSCR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15646_length_3733_cov_3.293094"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLT69_06615"
     CDS             complement(<1..275)
                     /locus_tag="PLT69_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ14144.1"
                     /translation="MKTPLFSEHERLGARIVDFHGWDMPVWYSGIRQEHLAVRRRAGV
                     FDVSHMGEIIVQGKNAPSFLNRMTTRNVAAMGVGTVLYTFLLNEGGG"
     gene            complement(399..1262)
                     /gene="folD"
                     /locus_tag="PLT69_06620"
     CDS             complement(399..1262)
                     /gene="folD"
                     /locus_tag="PLT69_06620"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOJ14145.1"
                     /translation="MAEIIDGKAIAMARREIAAKRAAEFAARYGRPPGLAVVLVGDDP
                     ASADYVKNKKRACADAGIASLEILLDASTTQEELVSHIERLNEEDGVDGILVQLPLPA
                     HLDSRDVLLAIDPAKDVDGFHPYNLGGLLAGEPSIVPCTPAGIMRLLEEYGVSPSGKD
                     AVVLGRSTIVGKPMALLLMMGNATVTICHSKTRDLPAKAASADILVAAVGRPEFVRGS
                     WIKPGAVVIDVGINRTPDGLKGDVHFQEALERASLITPVPGGVGPMTVAMLLENTVEA
                     AYRRIGRSPGL"
     gene            complement(1282..2346)
                     /locus_tag="PLT69_06625"
     CDS             complement(1282..2346)
                     /locus_tag="PLT69_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14146.1"
                     /translation="MVKRSRFELGHIEKIRRSIYLFLRDQLEHRLVFTALEGPYRLSR
                     VKGMPYPYVEPGDLRPKKKEFMKDSFVAQPFFIIFCEDTIDESHQKYIRFSDSNRVKK
                     ENLQLVPDINLTPSLYTTARYFERESFFELLEQLLDVDYCLLIQRDKSRGKGNSYCLT
                     HFHVKIDWPIADAAESLARELRYISKNLYEKGDRYAEILQQKLFEYYGCHHQAAGGRR
                     SAALIAAQYLKTIPYLSTVYVSSAETRTLTCYSEKGVGRYGIVQLDKQHAKAFLDAHA
                     VTENAFRQCYALGESENHYDVILFVRYEHTEWAQPPEDGRLRILKPDYSWLAVDTEMV
                     LPKTEAVNSRPLSIKWVYRS"
     gene            complement(2382..2750)
                     /gene="rnpB"
                     /locus_tag="PLT69_06630"
     ncRNA           complement(2382..2750)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLT69_06630"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2962..>3733
                     /locus_tag="PLT69_06635"
     CDS             2962..>3733
                     /locus_tag="PLT69_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOJ14147.1"
                     /translation="MRQADRFMNMYNHGFVRVGACIPAIKVADPSFNARSVARLVHEA
                     ARKRCVMAVFPELVLAGYTAEDLFFQDALLDSVLDALDTLKRETADIDMLVVVGAPLL
                     VVDSLFNCGVVFYRGRIAGVAVKSFLPNYREFYEKRQFRSARDLPVDVIGLCGQENVP
                     IGGDLIFEATGIPGFRAFVEICEDLWTPIPPSSYAALHGATVVANLSASNVTTGKDDY
                     RRSLVANQSARCIAAYLYTAAGQGESTTDLAWDGHAIVC"
BASE COUNT          590 a         1198 c         1220 g          725 t
ORIGIN      
        1 atccctcccc cctcgttcag gaggaacgtg tagagcaccg tgccgactcc catcgccgct
       61 acgttccgtg tggtcatgcg gttcaggaac gagggcgcgt ttttgccctg aacgatgatc
      121 tcgcccatgt ggctcacgtc gaacacgccc gcacgtctcc tcaccgcgag gtgctcctgc
      181 ctgatcccac tgtaccacac gggcatgtcc cagccatgga agtccacgat ccgggcccct
      241 aggcgctcgt gctcagagaa caaaggcgtt ttcatggaaa ccccgtcatc cattgcattg
      301 cccgcacagt caacgacccc tctttaacac gcaggtgcca agggcgcaag accgctcgga
      361 gaggggatcg ggaccgtcgc cggggcgcac cgtcgcacct agaggccggg agatctcccg
      421 atgcgcctgt aggcggcctc gacggtgttc tccaggagca tcgccacggt catgggcccg
      481 acgcccccgg ggaccggggt gatgagggat gccctctcga gggcctcctg gaaatggacg
      541 tcccccttca gcccgtccgg ggtccggttg atgcccacat cgatcaccac ggcgcccggt
      601 tttatccacg acccgcgtac gaactcgggc ctgccgacgg ccgccaccag gatgtccgcg
      661 gacgccgcct tggccggcag gtccctggtc ttcgagtggc agatcgtcac ggtggcgttg
      721 cccatcatga ggagcagggc catgggcttc ccgacgatgg tgctcctgcc gaggaccacg
      781 gcgtcctttc ccgagggcga gaccccgtac tcctcgagga ggcgcatgat gcccgccggc
      841 gtgcacggga cgatggaggg ctcgcccgcg agcaggccgc ccaggttgta cgggtggaac
      901 ccgtccacgt cctttgcggg gtcgatggcg aggaggacat ccctgctgtc gaggtgggcc
      961 ggcagcggga gttgtacgag gatgccgtcg acgccgtctt cctcgttcag ccgctcgatg
     1021 tgagagacga gctcctcttg ggtcgtcgag gcgtcgagga ggatctcgag ggaggcgatg
     1081 cccgcatccg cgcatgccct cttcttgttc ttcacgtagt cggccgaggc cgggtcgtcc
     1141 cccacgagca ccacggccag gcccggcggt ctcccgtacc tggcggcgaa ctcggccgcg
     1201 cgctttgctg cgatctccct tctggccatg gcgatggcct tgccgtcgat gatctcggcc
     1261 atacggtccc cccttgcctt gtcaactccg gtatacccac ttgatcgaca ggggcctcga
     1321 gttcaccgcc tcggtcttcg gcagcaccat ctccgtgtcc acggccagcc aggaatagtc
     1381 gggcttgagg atcctgagcc ggccgtcctc gggcggttgg gcccactcgg tgtgctcgta
     1441 cctgacgaac aggatcacgt catagtggtt ctcggactcg cccaatgcgt agcactggcg
     1501 gaacgcgttc tccgtaacgg cgtgagcgtc caagaacgcc ttggcgtgct gcttgtcgag
     1561 ttggacgatg ccgtacctgc ccacgccctt ctcagagtag caggtcagcg tcctcgtctc
     1621 ggcgctggag acgtagacgg tggagagata cgggatggtc ttgagatact gggccgctat
     1681 cagggccgcg gagcgcctgc cgcccgcggc ctggtggtgg cagccgtagt actcgaagag
     1741 cttctgctgg aggatctccg cgtaccggtc ccccttctcg tagaggttct tcgagatgta
     1801 cctgagctcc cgtgcgaggc tctccgcagc gtcggcgatg ggccagtcta tcttcacgtg
     1861 gaagtgggtc aggcagtagc tgttgccctt tcctcggctt ttgtcccgct ggatgagcag
     1921 gcagtagtcc acgtcgagga gctgttcgag gagctcgaag aaggactcgc gctcgaagta
     1981 ccgggcggtt gtgtacaggg aaggggtcag gttgatgtcg gggacgagct gcaggttttc
     2041 cttcttcacc ctgttcgagt cggagaagcg gatgtacttc tggtgcgact cgtcgatggt
     2101 gtcctcgcag aagatgatga agaacggttg ggcaacgaac gagtccttca tgaactcctt
     2161 cttcttgggc ctgaggtcgc cgggttccac gtagggatag ggcatcccct tgaccctgct
     2221 gagcctgtac gggccctcca gcgcggtgaa gacgagcctg tgctcgagct ggtccctcag
     2281 gaagaggtag atggacctcc gtatcttctc gatgtgcccg agttcgaacc gggatctctt
     2341 gaccatggga ggcccctcgt gcgcctggat gtactgcatg caggccgaag cagcacataa
     2401 gccgggttct gtcccccgcc gaatgcgcgg cggggtggta atcattcatc taggaccccg
     2461 gttgcccggg gcctcgagcg acctacccgg gggcgggacg ggcagcccca tgtgcccctc
     2521 tattggtctt gctccgggtg gggtttgcca agcttccccg gtcacccggg gaactggtga
     2581 gctcttacct cgccgtttca cccttgctgc cgcgccgggc agcggtctgt tttctgtggc
     2641 actttccctg gggtcacccc cggtcgccgt tagcgaccac ccttgccctg tggagcccgg
     2701 actttcctcc cgcgcctaag gcggcgccgg cgattacctg tgctccttcg gccagatatg
     2761 acgatctaca ggagtttcct cgggatttca agaaataagt ggagggacgt cccccgtgac
     2821 ccgcccgcat cgcggcggac gcactgggga cgggtgtctc cgggcgacag ggcagggccc
     2881 ttcatgtgtt tgatcgcgag gtccgatgag gtataatgac gtcgtgcccc aaggcggggg
     2941 cgaaaggagc tcggggggga catgcggcag gccgaccggt tcatgaacat gtacaaccac
     3001 gggttcgtcc gcgtcggcgc gtgcatccct gcgataaagg tcgccgaccc ctccttcaac
     3061 gcccgctccg tcgccaggct cgtgcacgag gccgcccgga agaggtgcgt gatggccgtg
     3121 ttcccggagc tggtgctggc cggctacacg gcggaggacc tcttcttcca ggacgccctg
     3181 ctggactccg tgctcgatgc cctcgacacc ctcaagcgcg agaccgcgga tatcgacatg
     3241 ctcgtggtcg tgggagcccc gctcctcgtg gtggactccc tgttcaactg cggggtcgtc
     3301 ttttaccggg ggaggatcgc gggcgtggcg gtgaagagct tcctgccgaa ctaccgtgag
     3361 ttctacgaga agaggcagtt caggtccgcg agggatctcc ccgtcgatgt catcggcctg
     3421 tgcgggcagg agaacgtccc catcggtggc gacctcatct tcgaggccac cggtatcccg
     3481 gggttcaggg cgttcgtgga gatctgcgag gacctctgga cccccatacc tccttccagc
     3541 tatgcagcgc ttcacggagc gacggtggtg gcgaacctct cggcgtccaa cgtcaccacc
     3601 ggcaaggacg attacaggag aagcctggtc gcgaaccagt ccgccaggtg catcgccgcg
     3661 tacctgtaca cggcggcggg gcagggcgaa tccacgacgg acctcgcgtg ggacgggcac
     3721 gccatcgtct gcg
//
LOCUS       DAOSCR010000228         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15752_length_3716_cov_3.845671, whole genome shotgun sequence.
ACCESSION   DAOSCR010000228 DAOSCR010000000
VERSION     DAOSCR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15752_length_3716_cov_3.845671"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLT69_06640"
     CDS             complement(<1..181)
                     /locus_tag="PLT69_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOJ14148.1"
                     /translation="MVGVVSDPVFMNHDTGGYHPESPMRVHYVHTVFEPGDPGILMVD
                     PVRADESDIRLVHSKG"
     gene            complement(191..559)
                     /locus_tag="PLT69_06645"
     CDS             complement(191..559)
                     /locus_tag="PLT69_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14149.1"
                     /translation="MPYDEAISNYPSVKGLSLELPFLKFRYLKYTGTRQAFAVNEPDP
                     AVASAPSRAAAVSRAPEARLVFRQEAPVTTMGMDPLVSFASVIHELAFGRVSPEGDEA
                     PSGVYPQAAHSPGRSIDFVV"
     gene            1331..2065
                     /locus_tag="PLT69_06650"
     CDS             1331..2065
                     /locus_tag="PLT69_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14150.1"
                     /translation="MRRVLRLAVAAFVLSVMASWAWSADFLADGNALYEKGKTSYEAY
                     KESGDAFAKALEADPKSYEAAWKAARSYREYANEAKKKNVANWKSICKEYGKLGMKYG
                     EKAIALNPNGVEGNFWFGCSVGNYSDAVSVLTALKEGLKDKTQKSFEKSYQLNKMYVD
                     GGPMKALGRFWFVLPWPLQDKKLSLQHLREFQKSFPNDPEGQVFLAEVLLKTGDKDEA
                     KVLLQKASQSSDKYFADWAKRLLSEM"
     gene            complement(2286..2642)
                     /locus_tag="PLT69_06655"
     CDS             complement(2286..2642)
                     /locus_tag="PLT69_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ14151.1"
                     /translation="MKEKRRYTRKPLKVEARYHDKKGTVLKGIVRNVSMGGIYIETSR
                     PLERGETIRLSLDAVDLGKVIDVEGRVVRSEAERGMGIEFTDHDNKDIKKLISTIRKL
                     EQASLLALSRAAMEAF"
     gene            complement(3107..>3716)
                     /locus_tag="PLT69_06660"
     CDS             complement(3107..>3716)
                     /locus_tag="PLT69_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14152.1"
                     /translation="IRVHSVAELHALPKIFTHMPPLKGPRIAVFTNSGAFGGITADLL
                     VQSGLSMARLSPETQERLARTGQIFNVKNPIDLGPALSMQTFLEIFDILLSSGEVDGL
                     LPVPSLWHPMVIDAVMELVKKCHEYGKPAAIYTPNAVAKTISYRSTYGVPLFESPEEA
                     VRALAVSYRHHRACVVKEEAKRMQPHAHGMSAPSRELLEESA"
BASE COUNT          642 a         1135 c         1232 g          707 t
ORIGIN      
        1 gccccttcga gtgcacgagc ctgatgtccg actcgtccgc cctgaccggg tcgaccatga
       61 ggatgcccgg gtctcccggc tcgaagaccg tgtgcacgta gtgcaccctc atgggcgact
      121 cggggtggta accgccggtg tcgtggttca tgaagacggg atccgagacg actcctacca
      181 tcaccttccc ctatacgacg aagtctatcg agcggcctgg tgagtgggct gcctgtggat
      241 agacgcctga gggagcctca tcgccttccg gggacaccct gccgaacgcc agctcgtgga
      301 tgacggaggc gaacgagacc aaggggtcca tgcccatggt cgtgaccggt gcctcctgcc
      361 tgaagacgag acgcgcctcg ggcgctctcg acaccgctgc agcccgggat ggggcggacg
      421 cgacggcggg atcgggctcg ttgacggcga acgcctgcct cgtgcctgta tacttgaggt
      481 acctgaactt caggaaggga agttccaagg aaaggccttt gacggacggg tagttcgaga
      541 tggcctcgtc gtagggcatc acccttgtct ataatgccga acgctctctt tggcaaggct
      601 tgatgacgac gcccggcgtg gtcccgatga acctccagtc actctggata gttggatctt
      661 caggcacaac ccgtttcgtg cctgcaccac ggagattcca tgagcatcgg gctcttcgcg
      721 ttcgcccacg gggtggccct ggagaccggg ggagaagggt ctcccggggc gggttcggcc
      781 tcattgtccc tcatgggcga ggagaaggac tcgggcggcg gcctgcggat cccccggtct
      841 accattgccc cgtacgggcg aggcgatgtg tccaacctca ccacgctgtc ctcggacagc
      901 gccccatgcc cctcatgggc gaggcgagga gcctcgaggt cgcgtctcgt gcctttcctg
      961 cgtaaggagg gcatccttgt aataacgctt cttctgcgcc agtgcctcct ttcctgcgcg
     1021 aggggggcat ccggtctccc gtggtaaaga accactgctt aggatgttcc tttcctgcgc
     1081 gaggggggca tcctcgtggc ctcttcatgt ggcacacgac ggccccgcct ccttttttgt
     1141 gcgggggggc atcctcgtgt tcgggggtct cgacatgaac aggccgcagg gaacccggtg
     1201 ttccgggatg aacgtcgccc cgcaagccct cagaggcccc ggccgatccc gggagacgaa
     1261 cctgttgatc gggctgggct ctggtggtag tgtctctgtg gtaccccatt tcccgcaagg
     1321 aggaattgac atgaggagag ttctgaggct cgcggttgca gcgtttgtgc tgtcggtcat
     1381 ggcatcatgg gcatggtctg cggatttcct ggcagatggc aatgcactct acgagaaggg
     1441 caagacgtcg tacgaggcgt acaaggaatc cggtgacgcc ttcgccaagg cgctcgaggc
     1501 agatccgaag agctacgagg ccgcgtggaa ggcggcccgg tcgtacaggg agtacgccaa
     1561 cgaggcgaag aagaagaatg tcgcgaactg gaagagcatc tgcaaggaat acggcaagct
     1621 cggcatgaag tatggcgaga aggcgatagc attgaacccg aacggcgtgg aggggaattt
     1681 ctggttcggc tgttccgtgg gcaactactc tgacgccgtg agcgtgctca ctgccctcaa
     1741 ggagggcctg aaggacaaaa cccagaagag cttcgagaaa tcctaccagc tgaacaagat
     1801 gtacgtcgac ggcggcccca tgaaggccct gggtcggttc tggttcgtgc tcccatggcc
     1861 cttgcaggac aagaagctct cgctccagca cctcagggag ttccagaagt ccttccccaa
     1921 cgaccccgag ggccaggtgt tcctggccga ggtcctgctg aagaccggcg acaaggacga
     1981 ggcgaaggtc ctgctgcaga aggcgtccca gtccagcgac aagtacttcg cggactgggc
     2041 caagaggctc ctctcggaga tgtgaactgg ccgaaggcgg gtgtcagggc tgaaagccgc
     2101 acccttctgg cctctgggcc ggccggcgac accttcctcc cggatagtat gctcaaggcc
     2161 gagacatgca ccggggcgtt tccccgaggg aaacgtcccg gtgttcatgc tggctttcga
     2221 gacggcacga ccttacctgt cgaaggtcgt gagtgcggtc gtggtatggc tgggaaaggc
     2281 ccgtgtcaga acgcttccat ggccgccctg ctgagggcga gcagggatgc ctgttccagc
     2341 ttgcgtatgg tgctgatgag cttttttatg tccttgttgt catggtccgt gaactcgatg
     2401 cccattcccc tttcggcctc ggaccgcacg accctgccct ccacgtcgat gacctttccc
     2461 aggtcgacgg catcgaggct caaccggatc gtctcaccgc gctcgagggg tctggacgtc
     2521 tcgatgtaga tccctcccat gctcacgttc ctgacaatgc ccttcaacac cgtgcccttc
     2581 ttgtcgtggt acctggcctc gaccttgagc ggcttccgcg tgtaacgcct cttttccttc
     2641 acggcatgtg tccccttgtg catcagggtg gatacaagtc ctcacgatcc tcttcgtcag
     2701 gttcgaggag aaacttgagc aggctgcgcg cacgggggcg ggtcgtatgt gtccttgttc
     2761 agcggtgccg gcgggggcgc agactgcacg cacggggggc gggtctcgat ccggcgcaca
     2821 tccggcgtgc atcccggcaa gggctcaagg ggttctcccc ctgcagggcg caaggagagc
     2881 gggagaccta ggcggcgtgc aggcgctcca gccgccggca ccggtactat ggtggagggc
     2941 cggataccgc cgcccctggt gcgcgccggt tcgcacaggc gccgcgatgc gataaacagg
     3001 gtggccgcct gtcgtgggtc cggccagaga gcggcgtcac gtcgctctct ggcaccgcct
     3061 cagggtctcc cgacggcttc cgagcaggag acggcagggg tcatgctcag gcgctctcct
     3121 cgaggagctc cctcgacggc gccgacatgc cgtgcgcatg gggctgcatc ctcttcgcct
     3181 cctccttgac cacgcaggcg cggtgatgcc tgtacgagac ggcgagcgcc ctcaccgcct
     3241 cctcgggtga ctcgaacagg gggacgccgt acgtgctcct gtaggaaatg gtcttggcca
     3301 ccgcgttcgg cgtgtagatc gcagcgggct tgccgtactc gtggcacttc ttcacgagct
     3361 ccatgaccgc atcgatcacc atggggtgcc ataggctcgg cacgggcagc agcccgtcca
     3421 cctcgcccga ggacagcagg atgtcgaaga tctcgaggaa ggtctgcatg gacagcgcag
     3481 gcccgaggtc gatggggttc ttcacgttga agatctggcc ggtgcgtgca aggcgttcct
     3541 gcgtctcggg ggagaggcgc gccatgctga ggcccgactg gacgaggaga tccgcagtga
     3601 tccccccgaa ggcgcccgag ttggtgaaca cggcgatcct cggccccttg agcggtggca
     3661 tgtgcgtgaa gatcttgggc agggcgtgca gctcggccac cgagtgcacg cggatg
//
LOCUS       DAOSCR010000229         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15756_length_3716_cov_2.969680, whole genome shotgun sequence.
ACCESSION   DAOSCR010000229 DAOSCR010000000
VERSION     DAOSCR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15756_length_3716_cov_2.969680"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1227)
                     /gene="trxB"
                     /locus_tag="PLT69_06665"
     CDS             complement(271..1227)
                     /gene="trxB"
                     /locus_tag="PLT69_06665"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOJ14153.1"
                     /translation="MYDTIIIGGGPAGLTAGMYASRSGLKSLLLASAFKPSLITTTDI
                     VENYPGFPGGISGHELVERFTEQATKFGLEIAQGDVSSISPRDGGGAPAWTIRTDDRE
                     FTGISVIVSTGTEYAHLGVPGEAEFTGRGVSYCATCDGPFYKDARLVVVGGGDTAIQE
                     ALYLTNFARTVTVIHRRDRLRATSVLQQRAFSNPKITFAWNSVVEEIVGDEVVKAVRT
                     RDTRDASLTRVIEADGVFIFTGMIPNTQAFRGVLDMDRSGYSIVDAAMKTSAKGVFAC
                     GDCTNKLLRQVVTACGDGATAAFAAYEYVSDLKGTAYTSYGS"
     gene            1338..1820
                     /locus_tag="PLT69_06670"
     CDS             1338..1820
                     /locus_tag="PLT69_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14154.1"
                     /translation="MKRMMFVWALVVLAPTAADAAGYVFATYGNGGDVGEPSYGIELG
                     AVFLSPYHPTGGAFSVGVGVSIADTDEDPPASPEKKYNDGSEQEVFASVGAELSPAFF
                     GVAGLGYSTQDVVKPYDSSDTENHATGMLGVRYVVRGFDIGLGYHTRRGVMVGVGAAF
                     "
     gene            1847..3250
                     /locus_tag="PLT69_06675"
     CDS             1847..3250
                     /locus_tag="PLT69_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14155.1"
                     /translation="MAGSTVRVLGFLALSCALALGGCASLENYTLRAAPARQYVAAGD
                     FQRAAEVFPERSARGRNEVLIRLERGMLLHEQGRFKESFDELDLAWRRMKEYEERAVV
                     SVSKTAAFAGTLAVNEQFSPYTGEDHEKVMLHAINAVNFLMLGDLEAARVEVLRAYAV
                     QRELSERHPPQAPDTQGGEAGATWEDSLRRADAEGYRTVSAKAAQVMSPYHNAYASIV
                     SALVYELTGEPGEAYIELKKAYEAFPASDYLANELLRLSAVLGYVEDRRAWELRFGRK
                     APPVETGASVVVFFSHGLGPTKEPVTLPIPLKNGVVFASLPVYRFTPSAVSHARVSVG
                     GRSTVTSPILDIDAVAARTLMDTFPGLFAKQVARSFLKARAVSGMAHDRNGLGAFFGT
                     LLAAATEQADLRTWSTLPKQIQASRIFVPAGERQVQVEAFPTGYRAAVEIPPGARCLV
                     VVCRHTDAGLSVHSKSY"
     gene            3273..3710
                     /locus_tag="PLT69_06680"
     CDS             3273..3710
                     /locus_tag="PLT69_06680"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HOJ14156.1"
                     /translation="MTRNKTVALLVMALGLAASCAGTAPNVLHVQLGPSGPGSTTTEI
                     NDGVLGRMLSFQEVTVKPVGGDGTRQVQVTIANTTSRDVAFEYRFVWYDGKGFEVSSL
                     AAWLPAVLGAGESRGFVSTAPGVDAASFRLMVRMPHGVTPAGT"
BASE COUNT          610 a         1195 c         1259 g          652 t
ORIGIN      
        1 cccttgtgct ttctcgcgag gttctcgagg atggggccca gcatcttgca cggcccgcac
       61 cactgtgccc agcagtcgac cacgaggatg gggtaggccg ctatggcacc gtccaggttc
      121 tgatcggtca cctcgatggg gtgatcgggg tccttcacga aggtatcgtc cgccatgtcc
      181 atcctcctgt atatctggct cttgtcccgt acgacccgcc cgcccggatc actgagcgcg
      241 ccccgagacg ggaaggtgtg cacaaccggc tcatgacccg tacgacgtgt acgccgtccc
      301 cttgaggtcg ctcacgtact cgtaggccgc aaaggccgcc gtcgcgccgt ccccgcatgc
      361 cgtcaccacc tggcgtaaca gcttgttcgt gcagtctccg caggcgaaga cccccttcgc
      421 cgaggttttc atggctgcat ccacgatgct gtacccggac ctgtccatgt cgagcacccc
      481 ccgaaacgcc tgggtattgg ggatcatgcc ggtaaagatg aagacaccgt ccgcctcgat
      541 cacccgcgtg agcgacgcat ccctggtgtc cctcgttctc acggccttca cgacctcgtc
      601 gcccacgatc tcctcgacca ccgagttcca cgcgaacgtt atcttcgggt tggagaaggc
      661 ccgctgctgg agcacggagg ttgcgcgcag ccggtccctg cggtggatga ccgtgaccgt
      721 cctcgcgaag ttcgtgaggt agagggcctc ctgtatggcc gtgtcgcccc cgcccacgac
      781 gacgagcctc gcgtccttgt agaatgggcc gtcgcaggtg gcgcagtagg atacacccct
      841 gcccgtgaac tccgcctcgc ccgggacccc gaggtgggcg tactcggtgc ccgtgcttac
      901 gatcacgctg atgcccgtga actcccggtc gtccgtcctg atcgtccacg caggggcacc
      961 cccgccgtcc ctaggggaga tcgagctaac gtctccctgg gcgatctcca gcccgaactt
     1021 cgtggcctgc tcggtgaacc gctcgacgag ctcgtggccg gatatgcccc cggggaagcc
     1081 ggggtagttc tccacgatat cggtggtggt tatgaggctc ggcttgaagg cgcttgccaa
     1141 gagcagcgat ttcaagcccg atcgagatgc atacatgccc gccgtgagac cggccggccc
     1201 cccgccgatg atgatggtgt cgtacacgtt agccccttcc ccccgggtgg aacggctgtt
     1261 cgggctgtcc accccgtgga ttttcgtcta tggtgttcgt actaggtaat agaaacgaaa
     1321 cgggtggagg tcaagccatg aagaggatga tgttcgtctg ggcgctcgtc gtcttggccc
     1381 cgacggcggc cgatgcggcg ggatatgtgt ttgcgactta cgggaacggc ggggacgtgg
     1441 gcgagccgag ttacggcatc gagctcggcg ctgtctttct gtccccgtat caccccacgg
     1501 gaggcgcctt cagcgtgggg gtgggcgtct cgatcgcgga cacggacgag gacccgcccg
     1561 catcccccga gaagaagtac aacgacgggt ccgagcagga ggtcttcgcg agcgtgggcg
     1621 ccgagctgtc gcccgccttc ttcggcgtgg ccgggttggg gtattcgacc caggacgtcg
     1681 tgaagcccta cgactcgtcc gacaccgaga accacgcgac gggcatgctc ggcgtgaggt
     1741 acgtcgtgcg tgggttcgac atcgggctcg gctaccacac gaggcggggt gtcatggtgg
     1801 gcgtgggcgc ggccttctga ttcatggtgg agggcccagg ggccctatgg ccggttcgac
     1861 cgtgagagtg ctcggatttc tcgccctttc gtgtgcacta gccctcggcg ggtgcgcaag
     1921 cctggagaac tacaccctga gagcggcccc tgcacggcag tacgttgcgg ccggtgattt
     1981 ccagagggcg gccgaggtct tccctgagcg ttcggccagg gggcgaaacg aggtgctgat
     2041 ccgcctggag cgcggcatgc tcctgcacga gcagggcagg ttcaaggaga gcttcgatga
     2101 gctggatctc gcatggaggc gcatgaagga gtacgaggag cgggccgtcg tgtccgtgtc
     2161 gaagacggcc gcattcgccg gcacgctggc cgtgaacgaa cagttttctc cctacaccgg
     2221 ggaagaccac gagaaggtga tgctgcacgc gataaacgcg gtgaacttcc tcatgctcgg
     2281 cgacctcgag gcggcgaggg tcgaggtcct gagggcgtac gcggtgcaac gcgagctctc
     2341 cgagaggcac ccgccacagg cgcccgacac ccaggggggc gaggccgggg ccacgtggga
     2401 ggattccctc aggcgggcgg acgccgaggg ctaccggacg gtgagcgcga aggctgcaca
     2461 ggtcatgagc ccctatcaca acgcgtacgc atcgatcgtg tccgccctcg tctacgagct
     2521 caccggcgag ccgggcgagg cgtacataga gctgaagaag gcgtacgagg ccttcccggc
     2581 gtcggactat ctcgcgaacg agctgctcag gctgagcgcc gtcctcgggt acgtcgaaga
     2641 caggcgggcc tgggagctca gattcggcag gaaggcgcct ccagtggaga ccggcgcgag
     2701 cgtcgtggtc ttcttctcgc acgggctcgg cccgacgaaa gagcctgtga ccctgccgat
     2761 ccccctgaag aacggcgtcg tgttcgcttc cctgccggtc taccggttca cgccgtcggc
     2821 ggtgtcgcat gcccgcgtca gcgtcggggg gcgaagcacg gtcacctcgc cgatactcga
     2881 catcgacgcc gtcgccgcgc gcaccctgat ggacacgttc cccgggctct tcgcaaagca
     2941 ggtcgccagg tcctttctca aggcccgggc ggtaagcggt atggcgcacg accggaacgg
     3001 cctcggggcc ttcttcggga cactcctggc cgccgcgacc gaacaggccg acctgaggac
     3061 ctggtccacc ctgcccaagc agatacaggc ctcacgcatc ttcgtgccgg ctggtgaacg
     3121 gcaggtgcag gtggaggcgt tccctacggg ataccgggca gcggtggaga taccgcccgg
     3181 cgcacgctgt ctcgtggtgg tctgcaggca cacggatgcg ggcttgagcg tccactcgaa
     3241 atcgtactaa agggagaagg gagggagtga tcatgaccag gaacaaaacg gtcgcactgc
     3301 tcgtcatggc cctcgggctc gcggcgtcgt gcgcggggac ggccccgaac gtcctgcacg
     3361 tccagctcgg tccctcaggg cccggctcca cgaccaccga gatcaacgac ggcgtcctcg
     3421 ggcggatgct gagcttccaa gaggtcaccg tgaaacccgt gggcggggac gggacacgac
     3481 aggtccaggt gacgatcgcg aacacgacct cgagggatgt cgcattcgag taccgcttcg
     3541 tctggtacga cggcaagggc ttcgaggtct cgagtctcgc ggcgtggctt cccgcggtgc
     3601 tgggtgcggg tgagtcacgc ggcttcgtgt ccacggcgcc cggtgtcgac gccgcgtcct
     3661 tcaggctcat ggtgcgcatg cctcacgggg tgacgcccgc aggcacctga ggggtg
//
LOCUS       DAOSCR010000230         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15798_length_3708_cov_3.140706, whole genome shotgun sequence.
ACCESSION   DAOSCR010000230 DAOSCR010000000
VERSION     DAOSCR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15798_length_3708_cov_3.140706"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PLT69_06685"
     CDS             <1..1624
                     /locus_tag="PLT69_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOJ14157.1"
                     /translation="RGCSRGCRFCQAGMIYRPYRERSLDTVLASFREGLSRTGYDNIA
                     MMALSRTDLSYLNELMNAMHCPSREVSVSIPSLRVEGITDEVARILASVRKPGFTMAP
                     EAATQRLRSVINKGNTEEDLFRSIRIIKALGWRSLKLYFMVGLPTEEDEDIEAICRLA
                     RDVGREFRGNVTVSVSCFVPKAFTPFQWEPQASQQRYNAVISHLQSRLRGRNITLKWH
                     DPRLSFLEGVFSRGDASLFEVLLEARARGAYLDGWGDTLKEGAWQDAFASRGIDPGEY
                     LAGRSLDADLAWDFIDMGITRDFLLRERALARQGLHTPDCRDGACPACGSCRDGAKNT
                     VRPAEPAFLLFEPSKPEPSLTYVFGITKEAGMRFLSPREFQETIRRAIRRAGIQAEYT
                     SGFSPVMKLSMTPPPPYAVSSLSEYFQVHLKEREDPASITERINDQLPEGARVFSARQ
                     GRLERVVACTYRTARPFTLEAGPGATITKAGTELKVWDFLEHVGQDTLRIAFIEGRTI
                     SPLAVLDAFSPDSIAPHEIVKTETHFRGDPAG"
     gene            2140..>3708
                     /locus_tag="PLT69_06690"
     CDS             2140..>3708
                     /locus_tag="PLT69_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ14158.1"
                     /translation="MCGMSTQDGEAFIPYEHAGVTYHFCCRACLEKFQKDPRAYTEKR
                     NARDARPVQGTPAGHGTYTCPMHPEVVLDHPGVCPKCGMALEPVAPSLDEGPDPEYED
                     MLGRFKVSLALSVVLMAVAMGHMIPGVREIQALSSGLRGWVEFAIATPVVVWAGWPFL
                     ARAWLSLKTLNLNMFTLIGGGVLVSYGYSATAVVAPWLFPESARPGGGGVGLYFESSA
                     MIVTLVLLGQVLELKARSRTQDAIRSLVSLAPKTARRIEEGGMEIDVPLDAIVPGDRL
                     RVRHGEKIPADGTIIEGRGVVDESMITGEPLPSDKGPGDWVTGGTLNTHGSFTMEARR
                     VGADTLLSSIVRVTMEAARSKAPVQRLADSVSGIFVPAVVATAALTFLAWVFYGPEPR
                     LANAVVSAVSVLVIACPCALGLATPMSIMVASGKAARMGILFKDASGVETLRKVDTFV
                     MDKTGTLTEGRPRVSGIAASDDTDEETILRYASSLALASDHPLSKAVLEEATARGITP
                     EPPSSIVTIPGMGVT"
BASE COUNT          692 a         1190 c         1218 g          608 t
ORIGIN      
        1 cagaggttgc tcaaggggtt gcaggttctg ccaggccggc atgatctaca ggccctacag
       61 agagcggtcc ttagacaccg tgctcgcctc gttcagggag ggactctcgc ggacgggcta
      121 cgacaacatc gcgatgatgg ccctgtccag gactgacctc agttacctga acgagctcat
      181 gaacgccatg cactgcccct ccagggaagt ctcggtgagc atcccttcgc tcagggtgga
      241 gggcatcacc gacgaggtgg cccgcatact cgcatccgtc agaaaaccgg gattcaccat
      301 ggcgcccgag gctgccaccc agaggctgcg ctcggtgata aacaagggga acaccgagga
      361 ggacctcttc aggagcatca ggatcatcaa ggcgctcggg tggaggtctc tcaagctcta
      421 cttcatggtg ggtctcccga cggaggagga cgaggacatc gaggcgatat gcaggctcgc
      481 gcgcgacgtg ggaagggagt tcaggggaaa cgtcacggtc agcgtgtcct gtttcgttcc
      541 caaggccttc acccctttcc agtgggaacc gcaggcatcg caacagagat ataacgccgt
      601 gatatcccac ctgcagtcca ggctcagggg caggaacatc acgctcaagt ggcacgaccc
      661 gcgcctgtct ttcctggaag gcgtcttctc ccggggcgac gccagcctct tcgaggtcct
      721 cctcgaggcg agggccaggg gggcctacct cgacggctgg ggtgacactc tcaaggaagg
      781 ggcgtggcag gacgcctttg cgtcccgtgg gatcgacccc ggagagtacc ttgcaggcag
      841 atcgctggat gccgatttgg cctgggattt catcgacatg gggatcacga gggacttcct
      901 cctgagggag cgtgctctgg ccaggcaggg cttgcacacc ccggactgca gggatggggc
      961 atgcccggcg tgcggctcat gcagggacgg cgcaaagaac accgtgaggc ctgccgagcc
     1021 cgccttcctg ctcttcgagc cgtcgaaacc cgagccttct ctcacgtacg tgttcggcat
     1081 cacgaaggag gcggggatgc ggttcctctc gcccagggaa ttccaggaga cgatccgcag
     1141 ggccataagg agggcgggca tccaggcgga atacaccagc gggttcagcc cggtcatgaa
     1201 gctcagcatg acccctccac cgccgtacgc cgtctcgtcg ctgagcgagt acttccaggt
     1261 gcacctgaaa gaacgggagg acccggcgtc catcacggag cgcatcaatg accagctgcc
     1321 cgaaggagca agggtctttt ccgccaggca gggccgcctg gagcgggttg tcgcctgcac
     1381 ctaccggaca gccaggccct tcaccctgga ggcgggaccg ggcgcaacga tcaccaaggc
     1441 gggcacagag ctcaaggtgt gggatttcct ggagcatgtc ggacaggaca ccctccgtat
     1501 agccttcatc gagggcagga ccatctcccc cctcgccgtc cttgacgcct tttcccccga
     1561 ctccatagcg ccccacgaga tcgtcaagac cgagacgcac ttcaggggcg atcccgccgg
     1621 ctgacaccgg gcaagggggt tccaacgcct gcacaccgtg ggccgatacg acacccttac
     1681 gctcgccagg gcagctgtca gggggtcgag aggcgcctgc aggcgcacga cggggtcttt
     1741 cactgtgcgc ccctggtccc cgggcgcaag aggaggcgtt cgccatgacc gaaacacggc
     1801 tgctgtccag gccccgcgtg cagccgtgaa ccgatgagcc ttgggccacg gccatgcatt
     1861 cgggaagaaa gcacgggcct ctcccgcccc gttcccctct ttcctccacg agccctgcac
     1921 gaaccgtgac gccggctgga gacgagaacc tgcgtgaggc ccttgccttc atccctcgcg
     1981 tgacctacta aaccacatac cccagatcga aaaggcggcc ttgtggaaga gaaaaagaaa
     2041 ttcttgcaga tccaagagaa gacggcgaag gaagctcacg ggtcgagccc gtgctgcgcg
     2101 cgcgggggag gtcagccttc cggcccgtat accgacccgg tgtgcggtat gagcacccaa
     2161 gacggcgagg cgttcatccc ctacgaacac gccggcgtga cgtaccactt ctgttgcagg
     2221 gcctgcctgg agaagttcca gaaagatcct agagcctaca cggagaagcg aaacgctcgg
     2281 gatgcgcggc ccgtccaggg gacgcccgcg ggacacggga catacacgtg tcccatgcac
     2341 ccggaggtgg tcctcgatca cccgggcgtc tgcccgaagt gcgggatggc cctcgagccg
     2401 gtcgcccctt cgctcgacga gggccctgac cccgaatacg aggacatgct cggccgcttc
     2461 aaggtgagcc tcgctctcag cgttgtcctc atggccgtgg ccatggggca catgatcccc
     2521 ggggtgaggg aaatacaggc cttgagttcg gggctcaggg ggtgggtaga gttcgcgatc
     2581 gcgacgccgg tggtggtgtg ggccgggtgg cccttcctcg cgagggcatg gctttccctg
     2641 aagaccctca acctcaacat gttcacgctc ataggcggag gcgtgctcgt gtcgtacggg
     2701 tacagcgcta cggcagtcgt agcgccctgg ctcttccccg agtcggccag accggggggc
     2761 gggggagtgg gcctgtactt cgagtcgtcc gcgatgatcg tgaccctcgt ccttctcgga
     2821 caggtgctgg aactcaaggc gagatcccgc acccaggacg cgatacgctc cctcgtgtcg
     2881 ctcgccccga agaccgcgcg caggatcgag gaaggcggca tggagatcga cgtgccgctc
     2941 gacgccatcg tcccgggcga ccgcctgcgc gtgaggcacg gggaaaagat ccccgcggac
     3001 ggcaccatca tcgagggaag gggggtcgtc gacgaatcga tgatcaccgg ggaacctctc
     3061 cccagcgaca agggcccggg cgactgggtg accggcggga ctctgaacac tcacggatcc
     3121 ttcaccatgg aggcacgacg ggtcggggcg gacacgctcc tctcatccat cgtgcgggtt
     3181 acgatggaag cggccaggag caaggcccct gtacagaggc ttgcggactc ggtgtcgggg
     3241 atcttcgtcc ctgcggtcgt ggcgaccgca gcgctgacct tccttgcgtg ggtgttctac
     3301 gggcccgaac cgaggctggc aaacgccgtc gtctcggccg tgtcggttct cgtcatcgcg
     3361 tgcccctgtg ccctcgggct cgcgaccccc atgtccatca tggtggcctc aggaaaggcc
     3421 gcgagaatgg ggatcctgtt caaggacgca tcgggggtcg agaccctgag aaaggtggac
     3481 accttcgtca tggacaagac cggcaccctg accgagggca ggccgcgtgt ctcgggcatc
     3541 gccgcctccg acgacacgga cgaggagacg atcttgaggt atgcatcgag ccttgccctc
     3601 gcgagcgatc accccctgag caaggccgtg ctcgaggagg cgaccgccag gggtatcacg
     3661 cccgagcctc cttcgtcgat cgtgacgatc cccggcatgg gcgtgacg
//
LOCUS       DAOSCR010000231         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15828_length_3702_cov_3.792158, whole genome shotgun sequence.
ACCESSION   DAOSCR010000231 DAOSCR010000000
VERSION     DAOSCR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15828_length_3702_cov_3.792158"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLT69_06695"
     CDS             complement(<1..463)
                     /locus_tag="PLT69_06695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ14159.1"
                     /translation="MDFSEIDALMSSADSSDRRAAANMLGDMCGEGTVERLVRLLKDT
                     NSGVRDAAYNALMFTGGSEAVEKVAPLVAETDPGVRNAAIDILRAIGDDAIDVLHRLA
                     RDANDDIRLFMLDILGTIGNPSSVGVLIEGLRDANPNVRNAAVVSLGLLGDP"
     gene            complement(520..1140)
                     /locus_tag="PLT69_06700"
     CDS             complement(520..1140)
                     /locus_tag="PLT69_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14160.1"
                     /translation="MAGEKARKADLDKEGIVLGDTSKAELDKEGITLAQEVDQDGAVG
                     DEPGTPQAEGKSEGGQEKVLGRGFFARYRLAVACGGAGALLAVVVAAAALIMSSSPEP
                     VSPQTHKAVHRKAQDGIVLDPFMVFYETHEDNRPGILVAQVSLKVDPSVVPTVMGNLY
                     DIRNIIYRRLAGAASAYSQTEIALMLSDDLLDYPIKEVAFLQYQAR"
     gene            complement(1153..1536)
                     /locus_tag="PLT69_06705"
     CDS             complement(1153..1536)
                     /locus_tag="PLT69_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HOJ14161.1"
                     /translation="MALDKNMKILVVDDFSTMRRIVKNILRQLNFVNIIEADDGSTAL
                     DVLQREKIDLIVSDWNMPKMTGLELLKAVRADDALKHIPFLMVTAEAQQENIIEAVKS
                     GVSNYIVKPFTAETLSQKINQIFSK"
     gene            complement(1593..2363)
                     /locus_tag="PLT69_06710"
     CDS             complement(1593..2363)
                     /locus_tag="PLT69_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ14162.1"
                     /translation="MQDIVYSELTEDQKLNPKVRSRIINEFAPLIKYIASRIAIRLPP
                     HIDLNDLINAGVIGLIDAIEKFDSSKQIKFKTYAEFRIRGAILDELRSMDWVPRSVRQ
                     KARKVEDAIAKLEYELGRPATDEEIAREMNVDMETFHKLLSETASVSLLSLDDLGEDE
                     TDLSKRNILEFIIQDERDWPSHRLRTAEISTLVAKAIQSLPEKERMVISLYYYDELTM
                     KEIGHVLKFTESRVSQIHTKAVLRLRSKMQKILKEISM"
     gene            complement(2332..3231)
                     /locus_tag="PLT69_06715"
     CDS             complement(2332..3231)
                     /locus_tag="PLT69_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOJ14163.1"
                     /translation="MKDQAHTLRKIFREGTLKAPEVISVTSGKGGVGKTSLVVNMGIL
                     LARRGNKVLILDADLGLANVDVMLGMAPRLTIKHVLDGRCSIDEVVLTGPGGIKIIPA
                     SSGIQELADLSPEQQMGLLDALDQLEDDFDYLIIDTGAGISRNVTYFNAASQRVIVVV
                     TAEPTSITDAYALIKVLRKQYGIKRFDLVTNNVNSKLEGDHVAQKLATVCDRFLGDVA
                     IEVLGSIPHDPSVPESIKAQKAFVEIDPRADASQRLLAVVERLAKTSRPSAGGNIQFF
                     FRRVLLNQVKGTSHAGHCVLGTY"
     gene            complement(3231..>3702)
                     /locus_tag="PLT69_06720"
     CDS             complement(3231..>3702)
                     /locus_tag="PLT69_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14164.1"
                     /translation="LDSYRIGAVDQSRIYARILNIPFFAVTTPGEFRSALSQLDSVDH
                     VLVDTVGRSPFCSQYIRQLEGFFEGVDACMFLLMPVATRDPEMDTVTRAFSPLKVDRM
                     IFTKADEASGFGGVVTHNMIHRIPISHITTGQRVPEDMEEARLETVIARTLGDA"
BASE COUNT          607 a         1171 c         1156 g          768 t
ORIGIN      
        1 cggggtcccc caggagaccg agcgagacca cggccgcgtt gcgcacgttc gggttcgcat
       61 ccctcaaccc ctcgatcagc acccccacgc tcgaggggtt cccgatggtg ccgaggatgt
      121 ccagcatgaa gagcctgatg tcgtcgttcg cgtcccgggc gagccggtgc agcacatcga
      181 tggcgtcgtc gccgatggcc cgcaggatgt ctatcgcggc gttgcgcacg cctgggtctg
      241 tctcggcgac caggggggcc accttctcca ccgcctcact gccgcccgtg aacatcagcg
      301 cgttgtaggc ggcgtccctc acgccgctgt tcgtgtcctt gagcagccga accagcctct
      361 cgaccgtccc ctcaccgcac atgtccccga gcatgttcgc ggcggcccgc ctgtccgagg
      421 aatcggcgga ggacatcaat gcatcaatct ccgagaaatc catgttcact cgtccccgtc
      481 acacccggtc tcaagaggga tcattccacc ccgaacccgt cacctggcct ggtactggag
      541 aaaggcgact tccttgatgg gatagtccag gaggtcgtcg gagagcatga gcgcgatctc
      601 ggtctgggag tacgcgctcg cggcccccgc cagcctccgg tagatgatgt tgcggatgtc
      661 gtagaggttg cccatgaccg tcgggaccac cgacgggtcg accttcaggc tgacctgcgc
      721 cacgaggata cccgggcggt tgtcctcgtg cgtctcgtag aaaaccatga acgggtcgag
      781 cacgatgccg tcctgggcct tcctgtggac cgccttgtgc gtctgggggg agacgggctc
      841 aggggaggag gacatgatga gcgcggccgc ggcgacgacc accgccagca gggcgccggc
      901 cccgccgcag gcgacggcga gccggtacct cgcgaagaaa cctcttccga gcaccttctc
      961 ctggccgccc tcggacttcc cttccgcctg cggcgttccc ggctcatccc caaccgcccc
     1021 atcctggtcg acctcttggg cgagggttat cccctccttg tcgagttcgg ccttcgaggt
     1081 gtcgccgagg acgatgccct ccttgtccag gtcggccttc ctcgcttttt cccctgccat
     1141 gtcgtcaccc gcctacttgc tgaaaatctg gttgatcttc tggctgagcg tctccgccgt
     1201 gaagggtttc acgatgtagt tgctcacgcc cgacttcacc gcctcgatga tgttctcctg
     1261 ctgcgcctcc gcagtgacca tcaggaaggg gatgtgcttg agggcgtcgt cggccctcac
     1321 cgccttgagc agctcgagac ccgtcatctt gggcatgttc cagtcggaga cgatcaggtc
     1381 tatcttctcg cgctggagca cgtcgagcgc ggtggacccg tcgtcggcct cgatgatgtt
     1441 cacgaagttg agctgcctga gaatattctt gacgatcctg cgcatggtgg agaagtcgtc
     1501 caccacgaga atcttcatgt tcttgtcgag agccatggga acctccgccg gtgtctcttt
     1561 tccagtatca tcgtaccgtt gggcccaaat ctttacatgg agatctcctt caggatcttc
     1621 tgcatcttgg atctcaggcg gagaaccgcc ttggtgtgga tctgggacac gcgggactcg
     1681 gtgaacttga gcacgtggcc gatctccttc atggtgagct cgtcgtagta gtacagcgag
     1741 atcaccatgc gctccttctc gggcagagac tggatggcct tcgccacgag cgtgctgatc
     1801 tccgccgtgc gcagcctgtg cgagggccag tcgcgctcgt cctggatgat gaactcgagg
     1861 atgttccgct tggacaggtc ggtctcgtcc tccccgaggt cgtccaggct gaggagcgat
     1921 acggaggcgg tctcggagag gagcttgtgg aaggtctcca tgtccacgtt catctccctg
     1981 gcgatctcct cgtccgtggc cggcctgccg agctcgtact cgagcttggc tatcgcgtcc
     2041 tccaccttgc gcgccttctg cctcaccgaa cggggtaccc agtccatgga cctgagctcg
     2101 tccaggatcg cgccccggat cctgaactcg gcgtaggtct tgaacttgat ctgcttggag
     2161 gagtcgaact tctcgatggc gtctatgaga ccgatcaccc cggcgttgat gaggtcgttc
     2221 aggtcgatgt gcggaggaag cctgatcgcg atgcggctcg cgatgtactt gatgagcgga
     2281 gcgaactcgt tgatgatcct cgagcggacc ttggggttga gcttctgatc ctcagtaagt
     2341 tccgagtaca caatgtcctg catggctcgt tcctttcacc tgattcaaga gcacccgcct
     2401 gaaaaagaat tggatgttgc ctcccgcgga cggccgggag gtcttggcca gcctttcgac
     2461 gacggcaagg agcctctgcg aggcatccgc acggggatcg atctccacga aggccttctg
     2521 ggcctttatg ctctccggga ccgacgggtc gtgggggatg gagccgagca cctcgatggc
     2581 gacgtctccc aggaaccggt cgcagacggt ggcgagcttc tgcgccacgt ggtctccctc
     2641 gagcttcgag ttcacgttgt tggtcacgag atcgaaccgc ttgatcccgt actgtttcct
     2701 gagcaccttg atgagggcgt acgcgtcggt tatggacgtc ggttccgcgg tgaccaccac
     2761 gatgaccctc tgggaggcgg cgttgaagta ggtcacgttc ctcgagatgc ccgcacccgt
     2821 gtcgatgatg aggtagtcga agtcgtcctc gagctggtcg agggcgtcca ggagccccat
     2881 ctgctgctcc ggggagaggt cagcgagttc ctggatgccg gatgaggcgg ggatgatctt
     2941 gataccgccg gggcccgtga ggacgacctc gtcgatggaa caccggccgt cgaggacgtg
     3001 cttgatggtg agcctcggcg ccatgccgag catcacgtcc acgttggcga gccccaagtc
     3061 cgcatccagg atgagcacct tgttcccccg ccgcgcgagg aggatcccca tgttcaccac
     3121 gaggctcgtc ttgcccacgc cgcccttgcc ggaggtcacc gatatgacct cgggggcctt
     3181 gagcgttccc tccctgaata tcttcctgag cgtatgcgcc tggtccttca tcaagcgtct
     3241 cccaatgttc gtgcgatgac ggtctcgagg cgggcctcct ccatgtcctc gggtacccgc
     3301 tgacctgtgg tgatgtggga aatggggatg cggtggatca tgttgtgggt cacgacgccg
     3361 ccgaacccgc tcgcctcgtc cgccttggtg aagatcatgc ggtccacctt caaaggcgag
     3421 aaggccctcg tgacggtgtc catctccggg tcacgggtgg ccaccggcat gaggagaaac
     3481 atgcacgcat ccacaccctc gaagaacccc tcgagttgcc gaatgtactg gctgcagaag
     3541 ggcgaccggc cgacggtgtc cacgagcacg tggtccaccg agtcgagctg ggacagcgcg
     3601 gagcggaact cgccgggcgt ggtcacggcg aagaagggga tgttcagtat cctggcgtag
     3661 atcctgctct ggtccaccgc gccgatacgg tagctgtcga gc
//
LOCUS       DAOSCR010000232         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15829_length_3702_cov_3.360570, whole genome shotgun sequence.
ACCESSION   DAOSCR010000232 DAOSCR010000000
VERSION     DAOSCR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15829_length_3702_cov_3.360570"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..647)
                     /locus_tag="PLT69_06725"
     CDS             complement(123..647)
                     /locus_tag="PLT69_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14165.1"
                     /translation="MSKIRSTLDIVMEKTKNLSMTEGEREEIRKKELASRVKGWVQGL
                     LDARHEIEDVKGFMRAESGAHQDVHDAVRRELLARIDPAGDNKRVLSLLGALTGIDTS
                     PIEKDLQAYREHLAGARTSFVSRASQRLASRGISGSAVVPNPDADPEWKAHLAQEGER
                     FLEGLKARYRLENQ"
     gene            complement(716..1624)
                     /locus_tag="PLT69_06730"
     CDS             complement(716..1624)
                     /locus_tag="PLT69_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ14166.1"
                     /translation="MDRMGVERSGIVLCGSNGITLAYESFGSEADPPVVLIMGLSSQM
                     VIWDDDFCTGLASRGFRVVRFDNRDVGLSSRFTHAGVPDIRTLISNPWGAKVSLPYTL
                     ADMAEDTVGLMDALDMGSAHVVGASMGGMIAQELAIRYPGRVRSLTSIMSTTGDPSLP
                     PPSPEALDMLFWPIPVDRNGYVEYFKRVWRLLSGPVYPMDELDLERLAALTYERGVEP
                     ASSARQFAAILAAPSRRKALASLDIPALVIHGDRDPLVHVECGRDTARAIPNARLVVV
                     EGMGHAISRPVWGLIIEELAGHFSRS"
     gene            2154..3503
                     /locus_tag="PLT69_06735"
     CDS             2154..3503
                     /locus_tag="PLT69_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOJ14167.1"
                     /translation="MTMRPQHDLPKIYSHAVESVDPYRLIRAGARLSGTKLSISGQGA
                     WVEEDLGRFRRIMVLGVGKASCRMARAVEEILGDRVEGGLVVTKYAHAEPLGRLPVVE
                     SAHPVPDENSLRFGLELARLAEGSDAPTLVVVLVSGGASSLCVLPCPGIRLEDKRKTT
                     DLLLSCGADIDEINCVRKHISMIKGGRLIRHLWPARTLSLILSDVVGDRLDTIASGIT
                     AADPTTYADAWGVLVRHGIEDKAPASVTALLRKGMEGLLPETLKENEAELSLVSNIIV
                     GNNRAACLAAKERGDTLGYNTRIITTTLTGDAVEQGHAFAELAVDIARGASDLARPAL
                     VVAGGETTVKVRGRGKGGRNQEMALAFLVGLLKAGVSHEGISFLSGATDGTDGPTDAS
                     GAMVTPELLSRVREHGPDPGPFLENNDSHGFFDAVGGLLVTGPTGTNVCDIQLLAVV"
BASE COUNT          652 a         1310 c         1112 g          628 t
ORIGIN      
        1 gcggcactca aggagagcat caagagactc aagtcgctcg acgcccagat cgtgctgccc
       61 ggccacggca ggctcctgaa gggacgcacg gccgtgaccc agaacttcgc cacgatcgag
      121 cattactggt tttcgagcct gtagcgggcc ttaaggcctt ccaggaaacg ctcaccctct
      181 tgcgccaggt gcgccttcca ttcggggtcc gcgtcaggat tcggcaccac ggccgacccc
      241 gatatccccc tcgaggcgag cctctgggag gccctcgaga cgaacgacgt ccttgccccg
      301 gcgaggtgct ccctgtaggc ctggaggtcc ttttcaatgg gcgaggtatc gataccggtc
      361 aaggccccca agaggctcaa gacccgcttg ttgtccccgg cggggtcgat gcgcgcaagc
      421 agctcgcgcc tcacggcgtc atggacgtct tggtgcgccc cggattccgc acgcatgaaa
      481 cccttcacgt cctctatctc gtgtctggcg tccaggagcc cctgcaccca gcccttcacc
      541 ctcgacgcca gttccttctt cctgatctcc tcacgctcgc cctcggtcat ggagaggttc
      601 ttcgtcttct ccatgacaat gtccagcgtg ctcctgatct tcgacatcgt gtgcctcctt
      661 caggcttgga tcctcccttc ccccgtttct tcaatagaca ccgtggcgca cataatcaag
      721 acctcgagaa gtgaccggca agctcctcga tgatcaggcc ccacacgggc ctcgagatgg
      781 cgtggcccat gccctcgacg acgaccaggc gcgcgttcgg tatggccctg gcggtgtccc
      841 tcccgcattc cacgtgcacc agggggtccc tgtcgccgtg aatgacgagt gcggggatgt
      901 ccagggaggc caaggccttc ctcctgctcg gggccgccag tatggctgcg aactgtctcg
      961 cgctcgaggc cggttcgacc ccccgctcgt acgtcaatgc cgcgagcctc tcgagatcga
     1021 gctcgtccat ggggtagacc ggcccgctca aaagcctcca gaccctcttg aagtactcca
     1081 cgtacccgtt tctgtccacc gggatgggcc agaagagcat gtcgagcgcc tcaggggaag
     1141 ggggcggcaa cgacgggtcg ccggttgtgg acatgataga ggtcagggag cggaccctgc
     1201 ccgggtaccg tatggcaagc tcctgcgcga tcatcccccc catggatgcg ccgaccacgt
     1261 gcgcgctccc catgtccagg gcatccatca ggcccaccgt gtcctccgcc atgtccgcga
     1321 gcgtgtaagg cagggacacc ttggcacccc acgggttcga gatgagggtt cttatgtccg
     1381 gcacgcccgc atgggtgaac ctgctcgaca gcccgacgtc cctgttgtcg aaccggacga
     1441 cccgaaaacc cctcgacgcc agtcccgtgc agaagtcgtc atcccagatc accatctggg
     1501 aactgagacc catgatgagt accacaggag ggtccgcctc actgccgaac gactcgtagg
     1561 ccaacgtgat gccgttcgat ccgcagagga cgatacccga tctctccact cccatcctgt
     1621 ccatgtcccc acctccttca tccggtgacc aatccctctg tcttgtgccg cctctctccg
     1681 ggcgccccgt ttcgaaggcg gcctgaggcg acgcgtatgc gcaggtcgga aaccgcgccg
     1741 cgaggagaga aacgacacgt gtcttttccc gaaaccgaca cccatccccg cgtagcgggc
     1801 gtcgaccacc cagccggcgc tctgcctgct cgaggcctcc cgccggaaag agcatccaca
     1861 ccgccctgca cgagcgcccc cggccccctc atgcgcaggc attcctttcg gacatgaaca
     1921 gggagagcga tctcctcggc agcgcctgag gtctggcgcg ggcgggctcg cggcgcacgc
     1981 accccgggcg ggctgagcac tcctctgccg cggcccggtg cgaaccggca cctgcccacc
     2041 cccctcgcat tttcctatcc atcccgacag ggccatggca tgacaacgat tcttgactca
     2101 caaaaccgcc cctgtcaaaa ggtatcaatg gcgagaggaa agggcccacc tgcatgacga
     2161 tgcgtcccca gcacgatctc ccgaagatct attcccatgc cgtggaaagc gtggacccgt
     2221 accgcctcat cagggccggg gcaaggcttt cgggtacgaa gctcagcata tcgggccaag
     2281 gcgcgtgggt ggaggaagac ctcggcaggt tccggaggat catggttctc ggcgtgggca
     2341 aggcgtcgtg ccggatggcg cgtgcagtgg aggagatcct tggcgaccgg gtcgaagggg
     2401 gcctcgtggt gacgaaatac gcccatgccg agccgctcgg gaggttgccg gtggtggagt
     2461 cggcccaccc ggtccccgac gagaacagcc tccgctttgg tctcgagctc gccaggctcg
     2521 ccgagggctc ggacgccccc accctcgtcg tggtcctggt ctcgggcggc gcctcctccc
     2581 tgtgcgttct cccgtgtcca ggcatacgcc tcgaggacaa gcgtaaaacc acggacctgc
     2641 tcctttcgtg cggcgccgac atcgacgaga tcaactgcgt gaggaagcac atctcgatga
     2701 tcaagggagg ccgcctcata cgacacctgt ggcctgccag gacgctctcg ctgatcctgt
     2761 cggacgtggt gggcgacagg ctcgacacca tcgcctcggg gatcaccgcg gctgacccca
     2821 ccacctacgc cgacgcgtgg ggggtcctcg tgagacacgg catcgaggac aaggcacccg
     2881 catcggtcac ggccttgctc cgcaaaggca tggaaggcct cctaccggag accctcaagg
     2941 agaacgaggc cgaactctcc ctcgtctcga acatcatcgt ggggaacaac cgggcggcgt
     3001 gcctggccgc gaaagagagg ggagacaccc tcggctataa cacgcgcatc atcaccacca
     3061 cgctcacggg cgacgcagtg gaacaaggcc atgccttcgc agagctcgcc gtcgacatcg
     3121 cccggggcgc atccgatctc gccaggcccg ccctcgtcgt ggcgggcggg gagaccacgg
     3181 tgaaggtcag gggcaggggc aaagggggaa gaaaccagga gatggccctc gcattcctcg
     3241 tcgggctcct caaggccggt gtgagccacg aggggatctc tttcctctcg ggcgcgaccg
     3301 acgggaccga cgggcccacc gatgcgtccg gtgcgatggt gacacccgag ctcctttcgc
     3361 gtgtccgaga acacgggcct gacccggggc cgttcttgga gaacaacgat tcccacgggt
     3421 tcttcgacgc cgtgggcggc ttgctcgtga ccggcccaac cggcacgaac gtctgcgaca
     3481 tccagctgtt ggccgtcgtg tgaccgtccg tcctcacccg cacaatcgcc gtccaggcca
     3541 acgaccttcc tcgtcggcat cccccaccgg cacgcccccc acggttcgca agcctcattc
     3601 atgggcatct ccacggccac gaccggcaca cgccatgccc catgaggtcg tttcgcgccc
     3661 gggccggtct ctctccagcc ataggacacc aggcggctgc gc
//
LOCUS       DAOSCR010000233         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15845_length_3699_cov_3.231888, whole genome shotgun sequence.
ACCESSION   DAOSCR010000233 DAOSCR010000000
VERSION     DAOSCR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15845_length_3699_cov_3.231888"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLT69_06740"
     CDS             complement(<1..690)
                     /locus_tag="PLT69_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ14168.1"
                     /translation="MRTLRVYAGPSALRLVRENGLQPDMVKVVAGAAGGPKWLVLCGL
                     DEAVFSSWLMDRASPVFLLGSSVGAWRFAALAQGMDAHRRFKEAYLAQRFSTVPTPSE
                     ASREIRKFIDAALEGNGSTRALSHPHARLSLLAVRCTGAFTTENRRILTALMAATALL
                     NAAARRALGLFFTRVLFHDPRDLPPYAHLEGFPLERVGLTPANLAQAVMASGSMPVLM
                     EGVRGIEGAPVG"
     gene            1389..1832
                     /locus_tag="PLT69_06745"
     CDS             1389..1832
                     /locus_tag="PLT69_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ14169.1"
                     /translation="MPTPRTARQGDKVKVHYRGRLEDGFEFDTSVGREPLVFIIGSGD
                     VIPGFEDGTTGMSVGEIKTITIEPDQAYGEHRDELLVEMPWEYFPEDIVPEVGMQLKL
                     VDESGEEIPVTVVNVDEEAVTLDANHPLAGHRLTFDIELLEIMEV"
     gene            complement(2150..3427)
                     /locus_tag="PLT69_06750"
     CDS             complement(2150..3427)
                     /locus_tag="PLT69_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOJ14170.1"
                     /translation="MAALALAGIALMAFLGAPIFTVLSSLALLGFSLSGLPVSALIVD
                     IYSQFSNNPVLYTIPIFTFAGFILAESKASVRVVNFSQAVLGWMPGGLAVVALVACAA
                     FTAFTGASGVTIIALGGLLFPVLVKGRYSEGFSLGLLTASGSLGLLFFPSLPIIIYGV
                     VAETSITQLFAAGIVPGLLLIVILSLVSAVYAVRARTETTRASLKNILKATSEAKWEL
                     LIPVILVVGIFGGFFTLGEVASIMVAYALLVEMFIHRDISLRQIPAIVRESMVLVGAI
                     FIIFSSALALTTYMIDAEIPMRILAFIQAHISSKVAFLLALNVFLLFVGCIMDIYSAL
                     VVVVPLIVPIATSYGVNPVHLGIIFLTNLEIGYLTPPVGMNLFISCIRFSRPVLEVCR
                     ASIPFILALLAGLALITYLPGLSLWLVEHFGIR"
     gene            complement(3429..3623)
                     /locus_tag="PLT69_06755"
     CDS             complement(3429..3623)
                     /locus_tag="PLT69_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14171.1"
                     /translation="MLLYASVGFIGVDYETGSTIAVLRFSIPTWALETVIPLGYGMIL
                     ARYAIRCAFNVRTLFGGRER"
BASE COUNT          695 a         1163 c         1295 g          546 t
ORIGIN      
        1 gccgacaggc gcgccctcga tgcctcgcac cccctccatg agcacgggca tggaccctga
       61 ggccatgacc gcctgggcga ggttcgcggg cgtgagcccg acccgctcga gggggaaccc
      121 ctcgaggtgt gcgtacgggg gcaggtccct ggggtcgtga aagagaaccc gggtgaagaa
      181 gagccccagg gccctcctgg ccgcggcgtt caacagggcc gtggcggcca tgagggctgt
      241 gaggatgcgc ctgttctcgg tcgtaaacgc gcccgtgcac ctcacagcca gcaggctcag
      301 gcgcgcgtgc gggtgggaga gcgcccgggt ggacccgttc ccctcgagcg cggcgtcgat
      361 gaacttccgg atctccctgc tcgcctcgct cggcgtgggg accgtcgaga agcgctgcgc
      421 gaggtaggcc tccttgaaac gcctgtgggc gtccatgccc tgggcgagcg ccgcgaatct
      481 ccacgcgccc accgaggagc cgaggaggaa gaccggggag gcccggtcca tgagccacga
      541 cgagaagacg gcctcgtcca ggccgcagag cacgagccac ttggggccgc ctgcagcccc
      601 ggccaccacc ttcaccatgt ccggctgcag gccgttctcc cggacgagcc tgagggccga
      661 agggcctgcg tacactctga gcgtcctcac gcgggttcct ttccttgtct cgtgatcccc
      721 tgaccttgcc ggagctctcg agggcgatgc cctgccgagt cccgcacgtc cccggtgcag
      781 ggcgcgccac accctgtgcc cccacaggcc cccccggcgg cgggaagggg agcggcccga
      841 tggtcgaccc cgccggggat gcggggtgag tggccatccc cccacgccgg ggtgaggggc
      901 cccgcccgtt gaggcggtct cgctttgcgc ccgagctccg ccttttcggt accccgggtg
      961 ttaccacacg gcatgtctcg ttgccaggga aacgtgacgt tccaggagaa ggccggggca
     1021 cggggccttt gccatgggct atgacccgtg cgtctccgcc ggtgggaacc acccgaggcc
     1081 gtgctgcata gccttgatgc ggccccttga cccggcgcgc cggtagccct ctggccggcc
     1141 ggcttctctg acgagggccg aaggcgcccc ctggggcagg gcgtgaaggg attccttccc
     1201 gaccaggggc gtgccggaag aggaccgagg ggtttggacc ccacaagacg cgtgagacgc
     1261 tcgaaaacga atcgagaccc cttcgtcatg gggtttcgag gcgcgatcct caaggacgat
     1321 ttgttgttgt gggcgggggc gtgttctgct aacttcaggt gatcgaccat cgaaaggggg
     1381 tgcggatcgt gccgacaccc aggaccgccc ggcagggcga caaggtcaag gtccactaca
     1441 gaggcaggct cgaggacggg ttcgagttcg acacgtccgt gggcagagag ccgctcgtgt
     1501 tcatcatcgg ctcgggtgac gtgatccccg ggttcgagga tgggacgacg ggcatgtccg
     1561 tgggcgagat caagaccatc accatagaac ccgatcaggc ctacggggag catcgggacg
     1621 agctcctcgt cgagatgccc tgggagtatt tccccgaaga catcgtgccc gaggtgggca
     1681 tgcagctcaa gctcgtggac gagagcggcg aggagatccc ggtgaccgtg gtcaacgtgg
     1741 acgaggaggc ggtgacgctg gacgccaacc acccgctcgc agggcacagg ctcaccttcg
     1801 acatagagct cctcgagatc atggaggtgt gacacctctc cggccgccgg tgccctggaa
     1861 atgcccccgc catgagggat gccggaggtt gtggaaaacc catgcactcc gtccgctgag
     1921 tcgccgcaca ggggaaggcc caacacccgc tggtcgggca ctgcacacgg gggagtgcat
     1981 gcttcccggt cgtgcccgca tgcctgccgg tcctttcagg ctgcgcggct tgccccggcc
     2041 ggcatggtgc gacgagcccc tcctcgctgc tgcaacgtct cacctgaagc cgggatgatc
     2101 gagtaccacg ggaacgccca cgtgcaaacg acgcaccatg ggtgcgccct cacctgatgc
     2161 cgaagtgctc gacgagccac aggctcaggc cgggcaggta cgttatgagg gcaaggcctg
     2221 cgagaagcgc gaggatgaac gggatggagg cccggcagac ctcgaggacc ggcctggaga
     2281 accgtatgca cgagatgaag aggttcatgc ccacaggcgg cgtgaggtag ccgatctcaa
     2341 ggttcgtgag gaagatgatc ccgaggtgca ccgggttgac cccgtagctc gtcgcgatgg
     2401 gcacgatgag ggggaccacg accacgagcg ccgagtagat gtccatgatg cacccgacga
     2461 agagcaggaa cacgttgagc gccagcagga acgcgacctt cgaggatatg tgcgcctgga
     2521 tgaacgccag gatgcgcatc gggatctccg cgtcgatcat gtaggtcgtc agcgccaggg
     2581 ccgagctgaa gatgatgaag atggcgccca cgagcaccat gctctcccgc acgatggcgg
     2641 ggatctgcct cagggatatg tccctgtgga tgaacatctc gacgaggagg gcgtaggcca
     2701 ccatgatgga ggccacctcg ccgagggtga agaaaccgcc gaagatgccc accacgagga
     2761 tcacgggtat gaggagttcc cacttggcct cgcttgttgc cttcaggatg ttcttgagag
     2821 aggcgcgcgt ggtctcggtg cgcgccctca ccgcgtatac cgcgctcacc agggagagga
     2881 tgacgatgag gaggagcccc ggcacgatgc ccgcggcaaa gagctgcgtg atgctggtct
     2941 ccgccacgac gccgtagatg atgatgggca ggctggggaa gaagagcagg cctagactgc
     3001 ctgacgcggt gagcagcccg aggctgaagc cctcggagta cctccccttg acgaggaccg
     3061 ggaagaggag cccgcccaag gcgatgatgg tcacacccga ggcccccgtg aatgcggtga
     3121 aggcggcgca ggcgacgagg gcgacgacag ccagcccccc cggcatccac ccgaggacgg
     3181 cctgggagaa gttcaccacc ctcacggatg ccttgctctc ggccaggatg aagcccgcga
     3241 aggtgaagat cggaatggta tagaggacgg ggttgttcga gaactggctg tagatgtcca
     3301 cgatgagggc tgacaccggc aacccggaaa gggagaagcc caagagggcg agcgaggaga
     3361 gcacggtgaa gatgggcgcc ccgagaaagg ccatcaacgc gatgccggcc aacgccaagg
     3421 cggccatctc acctctccct gccgccgaag agggtgcgca cgttgaacgc gcacctgatg
     3481 gcgtacctgg ccaggatcat gccgtagccc aggggaatga cggtctcgag ggcccaggtc
     3541 gggatggaga acctcaagac ggctatcgtg gagccggtct cgtagtcgac gccgatgaat
     3601 cccaccgatg cgtacaggag cactccgcag accccatggt gaagagcgag gtgacgatct
     3661 ccaggatggg cctcgcccgc tcaggcaaca ccctgtggg
//
LOCUS       DAOSCR010000234         3695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15872_length_3695_cov_3.679121, whole genome shotgun sequence.
ACCESSION   DAOSCR010000234 DAOSCR010000000
VERSION     DAOSCR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_3695_cov_3.679121"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLT69_06760"
     CDS             complement(<1..1120)
                     /locus_tag="PLT69_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14172.1"
                     /translation="MAGTVDFFSSSPEVGQATRDTRELEAVRAFFAALLLARKNCSLY
                     PKGHMVSRTSLEHCHALIEGYTRVHGSLRVEVGKDRLMSKGDVVLEDGQGDLSLPFVL
                     FRDGIRWIEFREGIGLGELEDFVETVNRYSLLTDESDGDIVTELWERKLEHIGYEVTE
                     AFWAEETCLEDHAESVEFDHEGHSEHAMQVIAAEEPDDPVVDVSSLGLTRQEEDIHRE
                     MVRLEEEGDHTAYLDALFDSLLQLRSMENYDLIFEVLKEEFSLSIAGADLDLACKILQ
                     RIRYVRDTCGIDEPWVASAADEFVREVSHERVLAGLVGVWTSLSDSQVERARDILEML
                     HPDALPALCTLLVLPQAASHRKVAEDVIAALACRDPGIL"
     gene            complement(1830..>3695)
                     /locus_tag="PLT69_06765"
     CDS             complement(1830..>3695)
                     /locus_tag="PLT69_06765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ14173.1"
                     /translation="LAYSLFKYNLSMALQHLRSVLFFTGTTVVLAGIAMAPVVALGLS
                     RSSLVAGVVLASLLNAPVRKGLDEVLSLFIPKASDRIHERYRLLTSNLTRVLRLEDLN
                     RHVASWMFETFRTTFVTSVFPGEGTDVGAAWTITNNEGMHRGLFGYTEEDPFSGCLGS
                     PAADERLLALCSEGRGVFSRDDLAGDARSAAGGFTDNRTIREVEIFMPVHSHGRLTAV
                     FLLGPKVDGSRYSRLEREVLTDLAVSLGPQVENARLLENLEQEVEKRTGELNEALVEA
                     MIKEREVAERNALIERQNRISEVLLETSTRMHQAKSLDDLFGFTLMQLKSLFEDLKGG
                     IVMVEKGRGIIDASAFVGLTEREQKLVMESVSAQGGPGFEGILAGKAERDVEGADKDA
                     HSQWKVFPLNRPGDRVMGYLILEGKDIHGPSERTVSLFVSLLSAVVQNRLLMADLEKM
                     ASTDGLTGVYNRAFLDRELEKSVKHAKRFKDMWFSVLIVDVNGLKQINDTYGHAAGDK
                     LIVEVASMLKSVCRETDVVARIGGDEFAVLMPSTNRVQAQILLKRIREAQADLNVVVS
                     PSVGVHLSIPVRISIGLASSDEDDPELVMKIADDLMYADKQRFYAEAGVAARIAR"
BASE COUNT          650 a         1262 c         1050 g          733 t
ORIGIN      
        1 cgaggatccc ggggtctcgg caggccaagg ccgcgatcac gtcctcggcc accttcctgt
       61 gcgaagccgc ctgcggcagc acgaggaggg tgcacagtgc gggcagggca tcggggtgga
      121 gcatctcgag gatatcccgg gccctttcga cctggctgtc ggacaggctc gtccagacgc
      181 ccacgagacc ggccagcacc cgctcatgcg agacctcgcg gacgaactcg tctgcagcgg
      241 atgcaaccca gggctcgtcg atcccgcatg tgtcccggac gtaccggata cgctgaagga
      301 tcttgcatgc gaggtcgagg tccgccccgg cgatcgacag ggagaactct tccttcaata
      361 cctcgaaaat gagatcgtag ttctccatcg acctcaactg gagcaggctg tcgaagagcg
      421 catccaggta tgccgtgtga tctccttcct cctcgagacg gaccatctcc ctgtggatgt
      481 cctcctcttg gcgggtgagc ccgaggctcg acacgtcgac cacggggtcg tccggttcct
      541 ccgccgcgat cacctgcatc gcatgttccg agtgcccctc gtggtcgaac tccaccgatt
      601 ccgcgtggtc ctcgaggcat gtctcttccg cccagaacgc ctcggtgacc tcatagccta
      661 tgtgttccaa cttcctctcc cagagctcgg tcacgatgtc gccgtccgac tcgtccgtga
      721 gcagcgagta cctgttgacc gtctccacga agtcctcgag ctcgccgagc cctatcccct
      781 ctcgaaactc gatccaccgg atgccgtccc gaaagagaac aaaggggaga gagaggtcac
      841 cctggccgtc ttccaggacg acatccccct tggacatgag cctgtctttt ccgacctcca
      901 cgcgcaggga tccatggacc ctcgtatagc cctcgatcag tgcatggcag tgctcgagag
      961 aggtcctgct caccatgtgg cccttggggt acaacgagca gttcttccta gccaggagca
     1021 gagcggcaaa gaatgcacgt acggcctcga gttccctcgt gtcccgcgtg gcctgcccca
     1081 cctcgggcga cgatgagaag aagtccacgg ttccagccat gttcacctca tgggcctcgt
     1141 tatgggtaac ccttcacctc aacccatacg ggtggagggg catccatcct cctgctcccc
     1201 gtgtttctct ccctctccag cgacgcacgg actcctgttt ttttctctgc atccaccctg
     1261 gatgccaggg agcgatctcg agcccatgta ttccttttcg gaagaacacg acgacacgat
     1321 gagcgaaagg cccagccggc atccttgccc catgaacctc aacaagcatg gatacctcca
     1381 cgctttcctc gttcgaccca ggtgcgtgat gcaggcgaat ccgctcaggg gcgtaatacc
     1441 tgcgcatgca tccacgatcg tgcgagaccg ccgcggcgac acagagttct tcccaggtgg
     1501 gaacccccac gtcgcgggtg cattgcccgc tcggggagag acgggttcac gtcgattcgt
     1561 cacggacgcg cccagggcgt acgctttccg ggtctcctct cacccggtcg cgtccttgcc
     1621 gcctgtgcgg tccttgacag gcgccctgcc cccctcacgc gccccttcac acgcaggcac
     1681 aaacgaaccc cggcagggca cgggatgcct cgatgccctg ccgggagctt ctttgacaat
     1741 gattgcctca ggtgagaacg aactcggcgg atcggggtgc ctgcatctcc aggcggggtc
     1801 cgccgcccgc gagacttgca tgctcccgct cagcgggcga ttctcgccgc aacccccgcc
     1861 tcggcgtaga agcgctgctt gtcagcgtac atgaggtcgt cggcgatctt catgacgagc
     1921 tcgggatcgt cctcgtcgga gctcgcgagc cctatgctga tccgtaccgg tatggataaa
     1981 tgcactccca cggacggcga cacgaccacg ttcaggtccg cctgagcctc cctgattctc
     2041 ttcaacagta tctgcgcctg caccctgttg gtcgagggca tgagaacggc gaactcgtcg
     2101 ccgcctattc gggccaccac gtctgtctcc ctgcacacgg acttgagcat ggaggccacc
     2161 tccacgatga gcttgtcacc tgcggcatga ccgtaggtgt cgttgatctg cttgaggccg
     2221 ttgacgtcga cgatcaagac cgagaaccac atgtccttga atctcttcgc gtgcttcacc
     2281 gacttctcga gctccctgtc caagaaggcc ctgttgtaga ctcccgtgag cccgtccgtg
     2341 gacgccatct tctccaggtc ggccatgagc agcctgttct gcacgaccgc ggaaagcagc
     2401 gagacgaaga gggacaccgt cctctccgac ggaccgtgaa tgtccttccc ctcgaggatc
     2461 aggtacccca tgaccctgtc cccgggacgg ttcaggggga agaccttcca ctgactgtgc
     2521 gcgtctttgt ccgcaccctc gacgtccctt tccgccttcc ccgccaggat gccttcgaac
     2581 cctgggcctc cctgagcgga aacgctctcc atgacgagct tctgctctct ctcggtgagc
     2641 ccgacgaaag cgctcgcatc gatgataccc ctgcctttct ccaccatgac gatcccgccc
     2701 ttgagatcct cgaacaggct cttgagctgc atcagcgtga acccgaagag gtcgtcgagg
     2761 ctttttgctt ggtgcatccg ggtgctcgtc tcgagaagga cctccgatat cctgttctgg
     2821 cgttcgatga gggcgttcct ctcggctact tcccgctcct ttatcatcgc ctctacgagg
     2881 gcttcattca gctcgcccgt cctcttctcc acctcctgtt ccaggttctc caggagcctc
     2941 gcgttctcga cctgcgggcc gagcgagacc gccaggtccg taaggacctc cctctcgagc
     3001 ctcgagtacc tcgagccgtc gaccttgggg ccgaggagga acacggccgt gagcctcccg
     3061 tgcgagtgca cgggcatgaa gatctccacc tcccggatgg tcctgttgtc ggtgaagccc
     3121 ccggctgcag agcgagcatc gcctgcgaga tcgtctctcg agaacacccc cctcccctct
     3181 gagcagagcg caaggagacg ttcgtccgcc gccggtgacc cgaggcaccc gctgaacggg
     3241 tcttcttcgg tatacccgaa gagccccctg tgcatgcctt cattgttggt gatcgtccag
     3301 gcggccccga catcggtgcc ctccccgggg aacaccgagg tcacgaacgt ggtcctgaac
     3361 gtctcgaaca tccacgatgc cacgtgtcgg ttcaggtctt cgagcctgag caccctcgtg
     3421 aggttggacg tgagcagcct gtaccgttcg tgaatcctgt cggaggcctt ggggatgaag
     3481 agcgagagga cctcgtccag gcccttcctc acgggtgcat tgagcaggct cgccagaacg
     3541 acgcctgcca cgagtgacga ccggctgaga ccgagggcga cgacgggcgc catggcgatg
     3601 cccgcgagca cgaccgttgt ccctgtgaag aacagcacac tcctgaggtg ctgaagggcc
     3661 atggacaggt tgtacttgaa caggctgtag gcaag
//
LOCUS       DAOSCR010000235         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15903_length_3690_cov_3.183219, whole genome shotgun sequence.
ACCESSION   DAOSCR010000235 DAOSCR010000000
VERSION     DAOSCR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15903_length_3690_cov_3.183219"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PLT69_06770"
     CDS             <1..1050
                     /locus_tag="PLT69_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ14174.1"
                     /translation="ISVYSPALTDFIFMVDKKSHMFITGPAIVKAVTGEELSKEDLGG
                     AGVHCVLSGVADGRFASEQDLLAGIRELMTYLPQNADERPPYLDLGDDPERPTDELMD
                     IVPSDASKAYDVRDAIACIVDAGRFFEVKPEFACEIVVGFGRLAGEVVGVVANQPSCL
                     AGSLTVDSSDKQTRFMRFCDCFNIPILLLVDTSAYLPGKDQEHRGIIRHGAKVLYALC
                     ESTVPRVGLVLRKAYGGGNLGMGVIPAQAGTDMMFYWPITELGVMGAKASVELFFGEA
                     IAKAPNPEETRAALMREYEDRYANPLREASYNPFINDVIEPGETRRVLVKAFAFLSTK
                     RKRPTYPKKHGNMPV"
     gene            complement(1215..2306)
                     /locus_tag="PLT69_06775"
     CDS             complement(1215..2306)
                     /locus_tag="PLT69_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14175.1"
                     /translation="MRVTPIAAVAFSAILLASFVLCRGQGEVPEALDMTGGHAGVYDL
                     ETIAARKVHHADGMFLNPFSDEPFHRRSFGQILRWKLFSKNRFEDEYARERVRPVSID
                     WAKVLSRSSTSVTWINHACVLVSDNGSRLIIDPVFFGLFWPIKDFSPIAFDVSSMPTV
                     DVVLVTHGHYDHLDIDSLLALKRGDPVYLVPLGFKGFLAQHGMNRVMEMDWFDTASAG
                     GFHVTFLPANHWTMRSIIEGPNRALWGSFLVKTPSGKTIYFSGDTAYFKAFDQIGRLG
                     AIDLAVFNVGAYEPRWFMKRSHLDPEEAVRAFRELGAKKFLAVHWGTFRLGDEPVFKP
                     GQDIRRVMAEQGLADRLVDLDHAGTFILE"
     gene            complement(2586..3446)
                     /locus_tag="PLT69_06780"
     CDS             complement(2586..3446)
                     /locus_tag="PLT69_06780"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOJ14176.1"
                     /translation="MEIKDIKTIGVVGSGQMGSGIAQVSAAAGFRVIMNDIGEHLVKK
                     GLGVIEKNLGRQLEKGSISRQEFDTILGRIALSTNLPDMKEADVVIEAATEDQVMKFR
                     IFSELDSVCPEHTILASNTSSIPIGRIAAQTKRPGKVIGMHFMNPVPVMKLCEIIRGL
                     ATTEETFRLTWDLAGVLGKTPAEANDYPGFISNRILMPMINEAVYCLYHGVGTRESID
                     TVMKLGMNHPMGPLALADLIGLDTCLAIMETLYQGFCDSKYRPCPLLRRYVEAGWLGR
                     KTKKGFYDYD"
     gene            complement(3464..>3690)
                     /locus_tag="PLT69_06785"
     CDS             complement(3464..>3690)
                     /locus_tag="PLT69_06785"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOJ14177.1"
                     /translation="AFAGSTVAVIRELGIDMDRVNVNGGSVALGHPIGSSGTRVLATL
                     LYEMIRSGKKTGVASLCLGGGEAVALVVTR"
BASE COUNT          671 a         1148 c         1186 g          685 t
ORIGIN      
        1 atctcggtgt actcaccggc actcaccgac ttcatcttca tggtggacaa gaagagccac
       61 atgttcataa ccggacccgc catcgtgaag gcggtcacgg gagaggagct ctccaaggag
      121 gacctcggcg gcgctggggt gcattgcgta ctctcagggg tggcggacgg gaggttcgcc
      181 tcggagcagg acctgctggc agggatcagg gagctcatga cgtatctccc gcagaacgcc
      241 gatgaacggc cgccttacct ggacctcggg gacgacccgg agaggcccac cgacgagctc
      301 atggatatcg tgccctcgga cgcatcgaag gcatacgacg tgcgcgacgc gatcgcgtgc
      361 atcgtggatg caggcaggtt tttcgaggtc aagcccgagt tcgcctgcga gatcgtggtg
      421 ggcttcggga ggctggccgg cgaggtggtg ggcgtcgtgg cgaaccagcc ttcctgcctg
      481 gccggcagcc tcacggtcga cagctccgac aagcagacac gattcatgcg gttctgcgac
      541 tgcttcaaca tccccatcct gctcctcgtg gacacctcgg cctacctccc gggcaaagac
      601 caggagcacc gggggatcat ccggcacggc gcaaaggtgc tctacgcgct gtgcgagtcg
      661 accgtcccga gggtggggct ggtcctgaga aaggcctacg gcggcggcaa cctcgggatg
      721 ggtgtgatac cggcgcaggc cggcacggac atgatgttct actggccgat caccgagctc
      781 ggggtcatgg gtgccaaggc gtcggtcgag ctcttcttcg gcgaggcgat cgccaaggcg
      841 ccgaacccgg aggagacgcg ggcggccctc atgagggagt acgaggacag gtatgcgaac
      901 ccgttgcgcg aggcgtccta caaccccttc atcaacgacg tgatcgagcc cggggaaacg
      961 aggagggtgc tcgtgaaggc ctttgccttc ctctcgacca agaggaagag gcccacctac
     1021 ccgaagaagc acggaaacat gcccgtctga aggccggggt gcgggactct gcaacgagag
     1081 tggagcatgg gggtcgaggg gagcggcccc tgggcgcacg cgtctggacg ccttcgggtt
     1141 gtctttcgcc ccaggcgcac gtcgaactgc acgcacgaac ggggagacac gcctcgacac
     1201 cctcgggctt tcagtcactc gaggatgaag gtccctgcgt ggtccaggtc gacgagccgg
     1261 tcggcgaggc cctgctcggc catgacccgc cgtatgtctt gaccgggttt gaacaccggc
     1321 tcgtccccga gcctgaaggt cccccagtgc acggcgagga acttcttcgc cccgagctct
     1381 ctgaacgccc tgacggcctc ttccgggtcg aggtgcgagc gcttcatgaa ccatcggggt
     1441 tcgtaggcgc ccacattgaa cacggcgagg tcgatggccc cgagcctgcc tatctggtcg
     1501 aatgccttga aataggcggt gtcgccggaa aagtagatgg tcttgcccga aggggtcttg
     1561 acgaggaaag agccccacag ggccctgttc ggcccctcga tgatgcttcg catggtccag
     1621 tggttggccg gcaggaaggt gacgtggaac ccccctgcgc tcgcggtgtc gaaccagtcc
     1681 atctccatga cccggttcat gccgtgctgt gcgaggaacc ccttgaaccc gaggggaacg
     1741 aggtagacag gatcgcccct cttcagcgcc aggagcgaat cgatgtccag atgatcgtag
     1801 tggccgtggg tcacgagaac gacatccacc gtgggcatgg acgagacgtc gaaggcgatg
     1861 gggctgaagt cctttatcgg ccagaagagg ccgaagaaga cgggatcgat gatgagcctg
     1921 gacccgttgt cgctcaccag gacgcaggcg tggttgatcc acgtgacgct ggtcgaggag
     1981 cgtgagagca ccttggccca gtcgatgctg accggcctga cccgttccct ggcgtactca
     2041 tcctcgaagc ggttcttgga aaagagcttc cacctgagga tctggccgaa ggaacgcctg
     2101 tggaacggct cgtcggagaa ggggttcaga aacatgccgt cagcatggtg gaccttgcgt
     2161 gcggcaatgg tctcgaggtc gtacacgcct gcgtgccccc ctgtcatgtc gagtgcttca
     2221 ggaacctctc cttggccccg gcacaagaca aaggaggcaa ggaggatcgc ggagaacgcg
     2281 acggcggcta tcggtgtcac tctcatgcaa ggcatcccac aggcgagtct ttgtatgaac
     2341 cctcgaggac cacatcccca tcggtccttc agccggggat gcgggagaac gacactgtct
     2401 tcaaccgcac agagtcctca ccacccttgt gcatgccgaa cctctccccc gggcggcgtc
     2461 gtgggtgcag gcaaggggtg acccatgccg tgggcttggg cccgacacac cgcactcctc
     2521 gacggcggtg ctgcccctgc cgcccatacg caccggttcc aacatagggt tctccgtgca
     2581 gggattcaat cgtaatcgta gaatcccttc ttcgtctttc tgcccagcca cccggcctcg
     2641 acgtacctgc gcagcagggg gcagggccgg tacttggagt cgcagaaccc ctggtagagg
     2701 gtctccatga tggccaggca ggtgtcaagg cctatgaggt cagccagggc gagcggtccc
     2761 atgggatggt tcatgccgag cttcatgacc gtgtctatgg attccctggt ccccaccccg
     2821 tggtagaggc agtagacggc ctcgttgatc atgggcatga ggatcctgtt ggagatgaac
     2881 cccgggtagt cgttcgcctc ggccggtgtc tttccgagga ctccggcgag gtcccacgtg
     2941 aggcggaagg tctcctcggt cgttgccagg ccccttatga tctcgcagag cttcatcacc
     3001 ggcactgggt tcatgaagtg catcccgatg accttgccgg gcctcttggt ctgtgccgcg
     3061 atcctgccta tggggatgga cgaggtgttg gaggcgagga tcgtgtgctc ggggcacacg
     3121 gagtcgagct cggagaatat cctgaacttc atgacctggt cttcggtggc ggcctcgatc
     3181 accacgtccg cctccttcat gtcgggcagg ttcgtgctca aggcgatgcg gccgaggatc
     3241 gtgtcaaact cctgcctgga gatcgagccc ttctcgagct gccttcccag gttcttttcg
     3301 atcaccccga gacccttctt cacgagatgc tcgccgatgt cgttcatgat gacccggaag
     3361 ccagcggccg ctgagacctg ggcgataccg cttcccatct ggcccgagcc tacgacccct
     3421 atcgtcttga tatccttgat ttccatgggg agaactcctc ctttcacctc gtgaccacca
     3481 gcgccaccgc ttcccctcca cccaggcaca ggctcgcgac gccggtcttc tttccggaac
     3541 ggatcatctc gtacaggagg gtggcgagca ccctggtgcc gctcgagccg atggggtgac
     3601 cgagcgccac gctgcccccg ttgacgttca ccctgtccat gtcgatcccg agttccctga
     3661 tgaccgccac cgtggaaccc gcgaatgcct
//
LOCUS       DAOSCR010000236         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_15993_length_3673_cov_2.846877, whole genome shotgun sequence.
ACCESSION   DAOSCR010000236 DAOSCR010000000
VERSION     DAOSCR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15993_length_3673_cov_2.846877"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="PLT69_06790"
     CDS             <1..1596
                     /locus_tag="PLT69_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOJ14178.1"
                     /translation="VAGVSPLTGFWGEATSGGDFPFRLKGAGYDGVFVTGRAEKPVYL
                     LVEDGTPSIRDAAHLWGKNAYETQEEIRRELGTKGLGMAAIGEAGERLVKTAGIQNDA
                     GRMAGRCGLGALMGSKNLKAVVASGNKRPPHADPAGVSRLAREAHETIRMNFLSIAYR
                     EYGTLLYSDMAMTLADVPARYFTKSVFPVEKVTGQALRQRYTVENYACAGCPIGCGRV
                     VKNFDGSHTIDGPEYETVMALGPLCMNFDLDSIIRANHLCNAHGIDTIAAGVAIAYAM
                     YLYEKGVISREEAGMEIRWGDGQAVVRLVEMIVKGEGIGRLLARGTLAMARHYGRDES
                     EAAQIKGMEIPMHDPRAFHGMAISYATGPRGACHLKGDYYSVELGTPVLEYMILPGDR
                     LSSEGKAESAAKYQNLKDLYDALTLCKFSPLSVTQICAIMKAVTGWEVTPDELLVTGE
                     RSINLKRVINNVLGVTSEHDRVPGICLEPLEEGSTAGTSPDMELMLKEYYEFRGWDRD
                     TGRPARDRLERLGLEKEAKLLYG"
     gene            1589..1867
                     /locus_tag="PLT69_06795"
     CDS             1589..1867
                     /locus_tag="PLT69_06795"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOJ14179.1"
                     /translation="MGEKDDLNCIVIHARDNVAVARVDIEAGGAVRPRPGESFPALER
                     IPCGHKVALVGIGMGEPVCKYGEIIGYATVDIAPGALVHTHNLKGDEP"
     gene            1864..3033
                     /locus_tag="PLT69_06800"
     CDS             1864..3033
                     /locus_tag="PLT69_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOJ14180.1"
                     /translation="MKTLPRTFMGYVRPDGSVGVRNHVAIIPTVACVNGVARMVEREV
                     GGAVAIIHGHGCGRAVEIGMHQGALAGIGANPNVAGAVVIGLGCETIDADWVASRIAE
                     KGKPVEVYRVQEHGGSLKTARKAADAARAMVGDLSLLERVECGIDSLVLGLECGGSDA
                     FSGVSANPAVGLVVDWVVDAGGSAILTESTEMIGTAHILARRAHDGETARQVEKVVDD
                     AWRRAQDILGPLAPFVVAPGNMDGGISSISEKSLGCICKAGSRSIQGVYGYGTRPERT
                     GLVIMDGPGYDMESMAGLASAGCQVILFTTGRGTPAGFPVVPVVKVASTSRLFAAMED
                     DMDVNAGTVLDGEPMEVVAGRIAARMLEIAGGQPSKAETNGMGGIVCLYAVTPSF"
     gene            3487..>3673
                     /locus_tag="PLT69_06805"
     CDS             3487..>3673
                     /locus_tag="PLT69_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14181.1"
                     /translation="MAVRGRKPLGLRRDGSDTVLVKRNYYIEDRYVRALAVLSALTGK
                     ELSLLVNEALQDLVQKYQ"
BASE COUNT          699 a         1108 c         1323 g          543 t
ORIGIN      
        1 gtggccgggg tctccccgct caccgggttc tggggtgagg ccaccagcgg cggggacttc
       61 cccttcaggc tcaagggcgc cggctacgac ggcgttttcg tgaccgggag ggccgaaaag
      121 cccgtctacc tcctcgtgga ggacggcacc ccctccatca gggatgcggc ccatctctgg
      181 ggaaagaacg cctacgagac acaggaggag atccgccggg agctcggcac gaagggcctc
      241 ggcatggcgg ccattggaga ggctggagag aggctcgtga agaccgcggg catccagaac
      301 gacgccggcc gcatggcggg caggtgcggt ctcggcgcac tcatggggtc gaagaacctg
      361 aaggcggtcg tggcctcggg caacaagagg cccccgcacg ccgacccggc aggggtatcg
      421 aggctcgcgc gcgaggcaca cgagaccatc aggatgaact ttttaagcat cgcctaccgc
      481 gagtacggca cgctgctcta cagcgacatg gccatgacgc tggccgatgt cccggcacgg
      541 tacttcacca agagcgtgtt ccccgtggag aaggtcacgg gccaggcgct gcgccagcgg
      601 tacacggtgg agaactacgc ctgcgcggga tgtcccatag ggtgcggacg ggtggtcaag
      661 aacttcgacg gctcacatac catagacggc ccggagtacg agaccgtgat ggccctcggc
      721 cccctgtgca tgaacttcga cctggactcc atcatacggg cgaaccacct ctgcaacgcc
      781 cacgggatcg ataccatcgc ggcgggcgtg gccatcgcgt atgccatgta cctctacgag
      841 aagggcgtga tctcgagaga ggaggcaggc atggagatcc gctggggtga cgggcaggcc
      901 gtcgtgaggc tggtggagat gatcgtcaag ggcgagggca taggcaggct gctcgcgcgc
      961 gggacgctcg ccatggcgcg ccactacgga agggacgagt ccgaggccgc ccagataaag
     1021 ggcatggaga tccccatgca cgaccccagg gccttccacg gcatggccat ctcctatgcc
     1081 acggggccgc ggggggcctg ccacctcaag ggtgactatt acagcgtgga actcggcacc
     1141 cccgtcctcg agtacatgat cctccccgga gaccgcctgt cgtcggaagg caaggccgag
     1201 agcgcggcca agtaccagaa cctcaaggac ctctacgatg cgctcaccct gtgcaagttc
     1261 tcccccttga gcgtgaccca gatctgcgcg atcatgaagg cggtcacggg gtgggaggtg
     1321 acgcccgacg agctcctcgt caccggcgag cgctcgataa acctcaagcg cgtcatcaac
     1381 aacgtactgg gggtcacctc cgagcacgac agggtccccg ggatctgcct cgagcccctc
     1441 gaagagggca gcacggcagg cacatcgccc gacatggagc tcatgctcaa ggagtactac
     1501 gagttcaggg gatgggacag ggacacgggc aggcccgcca gggaccgtct cgagaggctc
     1561 ggtctcgaga aggaggcgaa gctcctctat gggtgaaaag gacgacctca actgcattgt
     1621 cattcacgca cgcgacaacg tggccgtggc cagggtggac atcgaggcgg gcggggccgt
     1681 gaggccgcgc ccgggggaat cctttcccgc gctggagaga ataccgtgcg ggcacaaggt
     1741 ggcgctcgtg gggatcggga tgggggaacc ggtgtgcaag tacggcgaga tcatcgggta
     1801 cgcgacggtc gacatcgcgc cgggcgcgct cgtgcacacg cacaacctga agggggacga
     1861 accgtgaaga cccttcccag gacgttcatg gggtacgtga ggcccgacgg gagcgtgggc
     1921 gtgaggaacc acgtggccat catccccacg gtggcctgcg tcaacggggt ggctcgcatg
     1981 gtggagaggg aagtgggggg tgccgtcgcg atcatacacg gccacgggtg cgggagggcc
     2041 gtggaaattg gcatgcatca gggggccctt gccgggatag gcgcgaaccc caacgtggcc
     2101 ggcgccgtgg tgatagggct cgggtgcgag accatcgacg cggactgggt cgcatccagg
     2161 atcgcggaga agggaaagcc ggtcgaggtc tacagggtgc aggagcacgg cgggtcgttg
     2221 aagacggcgc gcaaggcggc cgatgcagcc cgcgcgatgg tcggcgactt gagcctcctc
     2281 gagcgtgtcg agtgcggcat cgactcgctg gtcctcggcc tcgagtgcgg tggctcggac
     2341 gcgttctcgg gcgtcagcgc caacccggcg gtggggctcg tcgtggactg ggtggtcgat
     2401 gccgggggaa gcgcgatcct caccgagagc acggagatga tcggcaccgc acacatcctg
     2461 gccaggcgcg cccatgacgg ggagaccgcg aggcaggtgg agaaggtcgt tgacgatgcg
     2521 tggaggcgcg cacaggacat cctgggtccc ctcgcgccct tcgtcgtggc gccgggcaac
     2581 atggacgggg gcatcagctc gatcagcgag aagtcgctcg ggtgcatctg caaggcgggc
     2641 agccggagca tccagggcgt gtacgggtac ggcacgaggc ccgagcgcac cggcctcgtg
     2701 atcatggacg ggccgggcta cgacatggag tccatggcag ggctcgcctc cgcgggctgc
     2761 caggtcatcc tgttcacgac cggcaggggg acgcctgcgg gtttccccgt ggtccccgtg
     2821 gtgaaggtcg cgagcacgag caggttgttc gcggccatgg aagacgacat ggacgtgaac
     2881 gcgggtaccg tgctcgacgg tgagccgatg gaggtcgtgg cggggaggat cgcggcccgc
     2941 atgctcgaga tagcgggcgg gcaaccctcg aaggccgaga cgaacggcat gggggggatc
     3001 gtgtgcctct acgccgtgac cccctctttc tgaacccctc ggccgcacaa aggccggacc
     3061 tcgatcctgg ggggagacct tcaccccttg acatgcacct gccgctgcgc ctttgggcgt
     3121 caccgtgagg tgcgggccgc accgcagccg gggccatgtc cttgcctgca ggcgggacgt
     3181 gaacgagaca ggcgtctccc ccctctcccc gccgcctcgt acggcacccc attgatccag
     3241 ccccaaagat aacgcattcg tgccggcgtg tctttcccct ggcccttatc gttgggagcg
     3301 cagagagaaa aacagacaaa tagagacatg acaatgcttg catgcatgag gaaaagtgaa
     3361 taaaatgtca ccatccaggc ggcgtcgggt ggcgtatcgg ggccgacgac atcgccctgg
     3421 cggcgatcgg ctcgagaagc cggctggcat gcgatgcgcg gaacgagcgc atagggaggt
     3481 cgggcaatgg ccgtgagagg gaggaaaccc ctggggttga gacgagacgg gtcagatacg
     3541 gtgctcgtca agagaaacta ttacatcgag gatcgctacg tgagggcgct cgccgtgctt
     3601 tcagccctga ccgggaagga gctctcgctg ctcgtgaacg aggcgctgca ggacctggtc
     3661 cagaagtatc agc
//
LOCUS       DAOSCR010000237         3667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16034_length_3667_cov_3.066168, whole genome shotgun sequence.
ACCESSION   DAOSCR010000237 DAOSCR010000000
VERSION     DAOSCR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16034_length_3667_cov_3.066168"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLT69_06810"
     CDS             <1..668
                     /locus_tag="PLT69_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOJ14182.1"
                     /translation="RTGRVTLVYREDMPFLVKLARLPSDHARPSGLSGRRAIRVVEPG
                     MVVRTVVHGGLLRNLTGDRFLAATRSLRELETSLILADRGIPTPEILAIRITRGLLFV
                     RLEVASRLVPEASDLLAFLEANPDDTREYLRKAGSLIRRMHDCGVYHADLHVKNLLLD
                     GSGVLYVIDLDKARAFDRLPTVLRRMNTNRFERSVRKWAKAGRLSAPHGWELSFRAGY
                     ERL"
     gene            complement(1044..1841)
                     /locus_tag="PLT69_06815"
     CDS             complement(1044..1841)
                     /locus_tag="PLT69_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ14183.1"
                     /translation="MKATFLRKCAILAVTVGSTLAISVYVLYLRLINAYDRDHADRVL
                     RWWSSLLLRATGATWEVHNPAAVKVPKGRPCIVMSNHRSHYDIPLIYVSLEGSIRMLT
                     KKELFRIPLWGRGMRAAEFISIDRSDNAQARKDLASAREKMEDGIVLWVAPEGTRSRT
                     GRLGAFKKGGFVLAIELGALIIPVGIRGSERIHRPGSSDIHVDQRVEVHIGEPVDASR
                     YSLEERDKLLEDVRTVISMLAGEHEPNLSPCAIQPETDGPTGLSIKR"
     gene            complement(2297..2686)
                     /locus_tag="PLT69_06820"
     CDS             complement(2297..2686)
                     /locus_tag="PLT69_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HOJ14184.1"
                     /translation="MDMTPRVYVWGFTDDERSRFSTFVSEMGVSRCEAIEPDQGNLMV
                     HEILFSDKTGPEPLQVDAKVVLFFNCQAETIHAIMKNAKTRDLPQPIYAMVTRENVDW
                     RFGDLVEHLKKEHEFVLRRMRQHKEEA"
     gene            2925..3296
                     /locus_tag="PLT69_06825"
     CDS             2925..3296
                     /locus_tag="PLT69_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ14185.1"
                     /translation="MRGMLFLASKGKKGLPMLCDIAQAEEAPRLLMAEIFQELSKKGL
                     VTSHVGICGGFANRRPPERGTPCGMVMAIDGHLAPKACLTGCGVSPLPGSCTAHPVWE
                     RFQALTRGIFSEVSLKSPVSP"
     gene            3376..>3667
                     /locus_tag="PLT69_06830"
     CDS             3376..>3667
                     /locus_tag="PLT69_06830"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOJ14186.1"
                     /translation="MDVVTLGRWQFAITSMFHFIFVPLTLGLSVLVAWMEWRFVKTGD
                     GTWLRMARFWGRLFVINFVLGVVTGITMEFQFGMNWAEYSKYVGDIFGAPAIE"
BASE COUNT          647 a         1172 c         1121 g          727 t
ORIGIN      
        1 cccgaacggg aagggtgacc ctcgtctaca gggaagacat gcctttcctc gtgaagctcg
       61 cgaggctgcc gtccgaccac gccagaccct ccgggctctc gggcaggagg gcgatacgcg
      121 tggtggagcc cggcatggtg gtgcggacgg tcgttcacgg cggccttctc aggaacctga
      181 cgggcgacag gttcctggcc gccacgcgct cgctcaggga gctcgagacg agcctgatcc
      241 tcgccgaccg cgggatcccc actcccgaga tcctggcgat caggatcacg cgtggtctcc
      301 tcttcgtcag gctggaggtg gcaagcaggc tcgtccccga ggcgagcgat ctcctcgcct
      361 tcctggaagc gaacccggac gacacccggg aatacctccg caaggcgggc tccctcatac
      421 ggcgcatgca cgactgcggc gtgtaccacg cggacctgca cgtgaaaaac ctgctgctcg
      481 acggctcagg cgtgctctac gtgatcgacc ttgacaaggc acgcgccttc gacaggctgc
      541 ccactgtcct gaggcgcatg aacacgaacc ggttcgagag gtcggtcagg aagtgggcca
      601 aggctggccg cttgtccgcc ccccatggct gggagctctc cttcagggcc ggttacgaga
      661 ggctgtgacc tcaggggcac ccagggccga caccaaggac gcgcctgccc cggcacaccg
      721 taggcccgtt cacacctgac atgccccaga tggggtcttt ctcgccacct tgcccacaga
      781 gtcgtcgatg gtgacccgtc tacacctgac ataccacaga tggggtctct ccgcctggag
      841 acgctcgccc cacccggcag gacgaacagg tacaccacgc ccggtccgca ggcctttacc
      901 cctcacgcgg ccgccaggtg tatcccccgc cctctccccg aggatctctc ttgacgaaac
      961 ggttctctcg agcccccttg gccgggcccg cccatgggcg gccgggatct ggagccgatt
     1021 tcaagaaagg ggagaaggaa cgatcacctc tttatggaca gtcctgtcgg tccgtcggtt
     1081 tcgggttgta tggcgcacgg ggagagattc ggctcgtgtt cgcccgcgag catggatatc
     1141 accgtgcgga cgtcctcgag cagcttgtca cgttcctcga ggctgtacct cgatgcgtcc
     1201 acgggctcgc ctatgtgcac ctccaccctc tggtccacgt ggatatccga gctgccgggc
     1261 cggtgaatcc gttctgaacc acgtatccca acgggtatga tcagggcccc aagctcgatg
     1321 gcgagcacaa agccaccctt cttgaacgcg ccgagccttc ctgtcctgct cctggtgcct
     1381 tccggcgcga cccagaggac gatcccgtcc tccatcttct ccctggccga ggcgagatcc
     1441 tttctcgcct gggcgttgtc ggaccggtcg atggagatga actccgccgc cctcatcccc
     1501 ctgccccaca ggggtatcct gaagagctcc ttcttcgtga gcatgcggat actgccctcg
     1561 agcgagacgt atatgagcgg gatgtcgtag tggctcctgt ggttggacat cacgatgcag
     1621 ggcctgccct tgggaacctt cacggctgcc gggttatgga cctcccacgt ggcacccgtc
     1681 gccctgagga gcagagaaga ccaccacctg agcaccctgt ccgcgtggtc ccggtcatag
     1741 gcgttgatca gccggagata gaggacatag accgagatcg cgagcgtgga ccccacggtc
     1801 acggccagta tcgcacattt tctcaagaat gtggccttca tggccgcttc aacctccttc
     1861 ttcaacatga ctgggcgtgt gatacgagct tgtgacgagc aggcactata cacgagcacc
     1921 tggtccagga aaagtaaaga aagtttaaaa ggagacccct tatactccgg aaacctgccc
     1981 gagacgcctt cgggtcgggg ctcagagaaa agggccggcc ctcatggcgg cctggtcgag
     2041 aggccccctt tctcacgacg agggtaaagc agttgtcccg tacgttgtgt acgaggcgca
     2101 ccatacacct ggagacgggc acgcggctcc cccgcacatt cccttcgccc gtctccaagg
     2161 tttacgcccc cttgggtatg ggctgggcat cccgaaaggg tggtgctgca aggtcggctt
     2221 cttccccgtc gatcgtggtg ggtttggagg gttggccatc gaggccgcca agaggtcagg
     2281 tgctgcgtgg ccggcttcag gcctcctctt tgtgttgcct catgcggcga agcacgaact
     2341 cgtgttcctt cttgaggtgc tccacgagat cgccgaagcg ccagtccacg ttctccctcg
     2401 tgaccatggc atagatgggc tgcggcaggt ccctcgtctt tgcgttcttc atgatcgcgt
     2461 ggatcgtctc ggcctggcag ttgaaaaaga ggacgacctt tgcatcaacc tggaggggtt
     2521 cagggccggt cttgtcggaa aacaggatct catggaccat gaggtttccc tggtcaggct
     2581 cgatggcctc gcatcgagac acgcccatct cggacacgaa cgtagagaac cgtgaccgtt
     2641 cgtcgtccgt gaagccccac acgtataccc tcggcgtcat gtccatgcga ccctcctgtc
     2701 cgtcccccat ccctgtacca tacccctgct gcgatctcac gcccattttt ctcaggtgga
     2761 gctcatccgc cggtgctacc ttcacatgat ctccatggat gtgaagacat cgagaggcga
     2821 tccaggtctt gactatcctt catggtggac tatgtataca ggacacgatc ggtctgctcc
     2881 gggtacacgt tgtgatggga ctgacgtgag aggcgggata cgcgatcagg ggcatgctct
     2941 tcctcgcatc gaagggaaag aagggccttc ccatgctctg cgacatcgcg caggcggagg
     3001 aggcccctcg tcttttgatg gccgagatct tccaggaact ctcgaagaag gggcttgtca
     3061 cgtcccatgt gggtatctgc ggaggcttcg ccaaccgcag gccgcctgaa cggggaacgc
     3121 cgtgcgggat ggtgatggcc atcgatggtc atctcgcacc gaaggcgtgt ctgacaggct
     3181 gcggggtatc ccccctcccg gggtcctgca ccgcgcaccc cgtgtgggaa aggttccagg
     3241 cgctcacccg tggcatcttc tccgaggtga gcctgaagtc cccggtctcc ccctgaggga
     3301 acgggacgcg ccaggcccgg gtgcgccgga aaggacgttt ttcctgggca ctaggtcgtc
     3361 gccagggagg ttttcatgga cgtcgtgact ctgggcaggt ggcagttcgc cataacgagc
     3421 atgttccact tcatcttcgt ccccctcacg ctcgggctgt cggtcctcgt ggcctggatg
     3481 gaatggcgtt tcgtcaagac cggcgacggg acgtggctga gaatggcgcg gttctggggc
     3541 aggctctttg tcatcaactt cgtcctgggc gtggtcacgg gaatcaccat ggagttccag
     3601 ttcggcatga actgggccga gtactcgaag tacgtcgggg atatcttcgg cgcaccggcc
     3661 atcgagg
//
LOCUS       DAOSCR010000238         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16051_length_3665_cov_3.104155, whole genome shotgun sequence.
ACCESSION   DAOSCR010000238 DAOSCR010000000
VERSION     DAOSCR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16051_length_3665_cov_3.104155"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLT69_06835"
     CDS             <1..223
                     /locus_tag="PLT69_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14187.1"
                     /translation="AFVAPREERRRRITLRARADDSPAEFDARDAREIAYGAAVPVAL
                     ADEYVLNTHSIDDAKASLEEIVSRRMGGA"
     gene            complement(954..2180)
                     /locus_tag="PLT69_06840"
     CDS             complement(954..2180)
                     /locus_tag="PLT69_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ14188.1"
                     /translation="MQFHDLALDERILKGIEDAGFTTCTEVQAASLPYCLDGRDVCVQ
                     SQTGSGKTAVFLITIFQRMMGMSNTKALIIVPTRELAVQVEREAGRLGAHLDYRTACI
                     YGGVGYKSQEQQLKLGANIVVGTPGRLLDLHRSGTLPFKDIGILVIDEADRLFDMGFI
                     KDLRKMLRAMPPAHKRQTMLFSATLSFLVKELAWQYMNDPVEIEIAPERLTVDTVTQE
                     LYHVSNEEKMSLLLGLLNREKPKNCMIFTNMKSTAENVARRLRANGIECEYITGDLPQ
                     KRRIEIIDALKTGRVPILVATNVAARGLHVDELDLVINYDLPEDCEDYVHRIGRTARV
                     GRHGKAISIACEDYVYSLEAIERYINMKIPVVWPDEGLFVEDRGSAERRPRMRGKRHP
                     REAAGRPGGRPKNRRR"
     gene            2445..2936
                     /locus_tag="PLT69_06845"
     CDS             2445..2936
                     /locus_tag="PLT69_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ14189.1"
                     /translation="MPIYEFYCSRCDTVYHFFSRSVNTTKVPMCPRCRKVRLKKKISM
                     FNAISGGRKSEDEPDLGGFDEAKMERAIQMLEREASGLDEDDPRQAAQLMRKLTEATG
                     LKLGSGMEEALSRMERGEDPDKIEAEMGDLLEEEEPFVLEQKSKGVKKRPGPKVDETL
                     YDL"
     gene            2991..>3665
                     /locus_tag="PLT69_06850"
     CDS             2991..>3665
                     /locus_tag="PLT69_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOJ14190.1"
                     /translation="MILSAARPYFCPHPGYFARILASDVFVVLDDVQFPLKTTWITRN
                     RFKNDRGVYWVRVPVLKKGLGLQKIRGVKVCPDERWRRKVLEGLRHAYGNAPYYEDHA
                     GALEEALSGPSDSLIDIDLSLIGYVLGETGISTRVVRMSETGVEPGGGPILVELCRKL
                     GASRIAVQGSGARTPDLDACERAGIELVHLKPCTPVYPQLWGGFIPNLSVFDLLFNCG
                     PRTSRYL"
BASE COUNT          610 a         1152 c         1240 g          663 t
ORIGIN      
        1 ggccttcgtg gcgccgaggg aggagaggag gcggcggatc accctcaggg cgcgggccga
       61 cgactcgccg gcggagttcg acgccaggga cgcgagggag atcgcctacg gtgcggccgt
      121 gcccgtcgcg ctggcggacg agtacgtcct gaacacgcac tcgatcgatg acgcgaaggc
      181 ctcgctcgag gagatcgtct cgaggaggat gggcggggct tagtcccagg caggcgcggg
      241 cctcggatgt gcgccgaaag acgctggagt gccggcaggc ccatggcggg gtgggttggt
      301 ttcaccgcgc accgggatgg atgggcacga acgggatgcc gaggtccggg aggtcagacc
      361 caagaccctg agcgcttacc accggacacc tttccttcgg ccgccgcttc tttcccgtgg
      421 cttttctccg ggcgtgcccc aggtcggtcc agccttgagg ccgaacgcca gcctgattcg
      481 ggcacaaggc gccctgtggg cgggcgccgt gcctctacag tacaggccgg aggctcgtgc
      541 ggtttgcgcc ggaaaggtcg atgggcctga gggcccggga ggggcgtggc cgggggccct
      601 cgatcgtcac ccctgacgtg gggcgggaag agagaggcga tgagggcgcc ttcaagccgg
      661 cggccgcccg gagatccttt cacgtgagag ggggagggtg ttttccaggg cgcgcagtca
      721 gcatacgacg tggattctcc cacgcgatag ggggaggctc ccggcgggga gccgaagacc
      781 cgctcctgtg ttcgacccgg cgcagggccc tgccggtcac ggcgggccga cttctaatgt
      841 tctgcaaggt cgcgccttgt gcccgggcct tcccgcaaac ggtctcaacg cggcggccgg
      901 gatcccgtcg ggcacgggtc tctccacccg tccgacagga agcggagccc gcgtcacctg
      961 cgcctgttct tggggcgccc gcccggtctg cccgccgcct cccgggggtg tctcttgccg
     1021 cgcatcctgg ggcgtctctc ggcagagccc ctgtcctcca cgaacagccc ctcgtcgggc
     1081 cagacgacgg gtatcttcat gttgatgtac cgctcgatcg cctcgagcga gtacacgtaa
     1141 tcctcgcagg cgatcgagat ggccttgccg tgcctgccga ccctcgcggt gcggccgatc
     1201 cggtgcacgt agtcctcgca gtcctccggc aggtcgtagt tgatcacgag gtcgagttcg
     1261 tccacgtgga gtcccctggc cgccacgttg gtcgccacga ggatggggac cctgcccgtc
     1321 ttgagcgcgt cgatgatctc gatgcgcctt ttctggggga ggtcgccggt gatgtactcg
     1381 cactcgatgc cgttcgccct gagccgtctc gcaacgttct ctgcggtgct cttcatgttg
     1441 gtaaagatca tgcagttctt gggcttctcc ctgttgagga gaccgaggag caggctcatc
     1501 ttctcctcgt tgctcacgtg gtagagctct tgcgtcaccg tgtccacggt cagcctttcc
     1561 ggcgctatct cgatctccac cgggtcgttc atgtactgcc acgcgagctc cttcaccagg
     1621 aagctcagcg tcgcggagaa gagcatggtc tgcctcttgt gggcgggcgg catcgccctg
     1681 agcatcttcc tgagatcctt gatgaacccc atgtcgaaga gacggtcggc ctcgtctatc
     1741 acgaggatcc cgatgtcctt gaaggggagc gtacccgagc ggtggaggtc caggagacgt
     1801 cccggggtgc cgacgacgat gttcgccccc agcttgagct gctgttcctg gctcttgtac
     1861 ccgacgcctc cgtagatgca ggcggtacgg taatccaggt gagcgccgag gcggccggcc
     1921 tcgcgctcca cctgcacggc gagttcacgc gtcgggacga tgatcagggc cttggtgttc
     1981 gacatcccca tcattcgctg gaagatggtg atgaggaaca cggcggtctt tccgctgccc
     2041 gtctgcgact ggacgcagac atccctcccg tcgaggcagt acggcagcga tgccgcctgc
     2101 acctcggtgc acgtggtgaa gcctgcgtct tcgatgcctt tcaggatgcg ctcgtccagc
     2161 gctagatcgt gaaactgcat gggaactcct cgaatgggtt gagcatctgt gcgggtgcct
     2221 tcatggatgg ttacgggcga tcaatgtctt gcgggatcat agcactcctg tgcgaaatgg
     2281 caaggataaa aacccgtgcg ccgcatgggc cggcatggcg cggccgcctg gggccggcgc
     2341 cccctgcccg gttcactgca ccgcacatcg tcccgcttga tttttccggc gcgaaatgat
     2401 ccttataatg ttctcggaac aagcgtcttc acaggggggc ggttatgccc atatacgagt
     2461 tctattgctc gaggtgcgac accgtctacc atttcttctc gaggagcgtg aacaccacca
     2521 aggtccccat gtgcccgagg tgcaggaagg tcaggctcaa gaagaagatc tccatgttca
     2581 acgccatctc cggggggagg aagtccgagg acgagcccga cctgggcggt ttcgacgagg
     2641 cgaagatgga aagggcgatc cagatgctcg agcgcgaggc gtccggtctt gacgaggacg
     2701 atcccaggca ggccgcgcag ctcatgagga aactcaccga ggccacgggc ctcaagctcg
     2761 gctcgggcat ggaagaggcc ctgagcagga tggaacgcgg cgaagacccc gacaagatcg
     2821 aggccgagat gggggatctc ctcgaggagg aagagccgtt cgtcctggaa cagaagtcga
     2881 agggcgtcaa gaaacgcccg ggcccgaagg tcgacgagac cctctacgac ctctaggatc
     2941 gccctcaccc gtaccggcct tgcgggcgga aaggagtgcg tggggagacc gtgatactgt
     3001 cggcggcgag accgtacttc tgcccccatc ccggctactt cgccaggata ctggcgagcg
     3061 acgtcttcgt ggtcttggac gatgtccagt tccccctgaa gacgacctgg atcacgcgca
     3121 acaggttcaa gaacgaccgg ggggtctact gggtcagggt gccggtgctc aagaagggtc
     3181 tcgggctcca gaagatacgc ggggtcaagg tctgccccga cgagaggtgg cgccgcaagg
     3241 tgctcgaggg cctcaggcac gcatacggca acgcccccta ctacgaggac cacgcggggg
     3301 ccctcgagga ggcgttgagc ggcccttcgg acagcctcat cgacatcgac ctgtccctca
     3361 tcgggtatgt cctcggcgag acgggcatct ccacgagggt cgtgaggatg tccgagacgg
     3421 gtgtcgagcc cgggggcgga ccgatactcg tggagctctg caggaagctg ggcgcctcgc
     3481 gcatcgccgt gcaggggtct ggggcgagga cgcccgatct cgacgcatgc gagcgcgcag
     3541 gcatcgagct cgtgcacctc aagccgtgca cgcccgtcta tccccagctc tggggaggat
     3601 tcatcccgaa cctctccgtg ttcgacctcc tcttcaactg cgggccccgc acctctcggt
     3661 acctc
//
LOCUS       DAOSCR010000239         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16154_length_3647_cov_3.487751, whole genome shotgun sequence.
ACCESSION   DAOSCR010000239 DAOSCR010000000
VERSION     DAOSCR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16154_length_3647_cov_3.487751"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLT69_06855"
     CDS             <1..264
                     /locus_tag="PLT69_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOJ14191.1"
                     /translation="DRIRENVKKEAANLDLAHVMSQSISQTLSRTVLTSFTVFLVALC
                     LFLLGGNVIHDFAFAMIVGVVTGTYSSIYIASPVVLAVSRGKK"
     gene            complement(528..2222)
                     /locus_tag="PLT69_06860"
     CDS             complement(528..2222)
                     /locus_tag="PLT69_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ14192.1"
                     /translation="MESLKLDGKHHDRRIESTPEYMRKLFIMPDSQDRFVEFGVHLLD
                     MIHDFFKEKGGIHSSISMEELAGIFSNIEMPGEPRLIKDVLDEIKNNIIRHSVKVANP
                     YYIGHMTSAIPYFMILLEMIAVSLNQNQVKIESAKASTFVEREFLCWIHHLIYDNPPE
                     FYHRHIQNPEVALGNITSDGTMANLTALTLAMAKAFPPDRKGFRGVRVEGLARALAHY
                     GYRQALFLVSKRGHYSICKAGTILGIGEQGVVHVPVHPVLNTIDLGKLTCIIDRIRKE
                     DKATGQPTKFVALVGIAGTTETGNIDDLEALAEIAKELGAHFHVDAAWGGGALLMEGS
                     RPMMKGIEKADTVTMDAHKLLYAPNSMGICVFKNAEDSRYLYHTSNYIIREGSVDQGR
                     FTIEGSRSFACLKPWAAIKIIGRHGYKLIFDHARELQDTFVQFIKRDRFFELMNDPEL
                     FIINYRFVPEELGAELNDLMKNPRGNSERITAINKVINAINIELHKTIRDHDMSFVSR
                     TRLESTRYAPRKIVVLRAITINPNTERYMLRQILNEHRSMGIRIWNGMKKDRRTGV"
     gene            2331..3146
                     /gene="rsmI"
                     /locus_tag="PLT69_06865"
     CDS             2331..3146
                     /gene="rsmI"
                     /locus_tag="PLT69_06865"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ14193.1"
                     /translation="MVPTPLGNLKDVTLRVLDVLAAVDLVLCEDTRRTRKLLSHYRLS
                     KQLVSCERFSEARRVSYVLGALGEGKSLALVSDAGMPGISDPGTRIIQSVRREGYEVV
                     VLPGPSAIVTAFAASGIDKPFRFIGFLPRKTEEKRLAVHAMLSSRDATVFYESPRRLL
                     STLKELAAGDPGRFVCIAREMTKIHEEYLAGSAREVLSQLEGKDVRGELTVVVDGSSR
                     DAEPSWDAIDRAGMRLLALGVPLKEASAVLADVSGLSRTELYRRLLASCRKGP"
BASE COUNT          589 a         1100 c         1242 g          716 t
ORIGIN      
        1 gacaggatcc gggagaacgt gaagaaggag gccgcaaacc tcgacctcgc ccatgtcatg
       61 agccagagca tcagccagac cctgagcagg accgtgctca cctcgttcac cgtgttcttg
      121 gtggcgctct gcctgttctt gctgggcgga aacgtcatac acgacttcgc gttcgccatg
      181 atcgtgggcg tggtcacggg aacctactcc tccatctaca tcgcgagccc ggtcgtgctg
      241 gccgtgagca gggggaagaa gtagggtcag cggtgcggcc caggccggta ggccccctga
      301 accgccgata cccggggaga aggcctcagg gatggggggg cccaaggcgc ggggtgcagt
      361 ggcgccctgc cctgggtgga ggggctttgc cctcggcagg ggtgggcctc cgcggccttt
      421 tgaggtgttg cgcgttcccc cgggggcctg gggtgcaggc cttgtgcgtg caaggggaag
      481 gggtctcctg ccgtgagtcc cccggggccg ggggtgcggg ggatcttcta cacgccggta
      541 cgccggtctt tcttcatgcc gttccagatc ctgatgccca tgctcctgtg ctcgttcagg
      601 atctgcctga gcatgtagcg ctccgtgttc gggttgatgg tgatggcgcg gagcacgacg
      661 atcttcctcg gcgcatacct cgtggactcg agcctggtgc gcgagacgaa ggacatgtcg
      721 tggtccctga tcgtcttgtg gagctctatg ttgatggcgt ttatgacctt gttgatggcg
      781 gtgatccgct cggagttgcc ccgggggttc ttcatgaggt cgttgagctc ggccccgagc
      841 tcctcgggaa cgaaacggta attgatgatg aagagttccg ggtcgttcat gagctcgaag
      901 aaccggtcgc gcttgatgaa ctggacgaag gtgtcctgga gttccctggc gtggtcgaag
      961 atcagcttgt agccgtgcct gcctatgatc ttgatggcgg cccacggctt caggcacgcg
     1021 aacgagcgtg agccctcgat ggtgaagcgg ccctggtcca ccgatccctc gcggatgatg
     1081 tagttggagg tgtggtagag gtaccgcgag tcctcggcgt tcttgaacac gcagatcccc
     1141 atgctgttgg gcgcgtagag gagcttgtgg gcgtccatgg tgaccgtgtc cgccttctcg
     1201 atgcccttca tcatgggtct cgagccctcc atgaggagcg caccccctcc ccaggccgcg
     1261 tccacgtgga agtgcgcccc cagctccttg gcgatctcgg cgagcgcctc caggtcgtct
     1321 atgttgcccg tctccgtggt gccggcgatg cccaccaggg ccacgaactt ggtgggttgc
     1381 cccgtggcct tgtcttcctt ccggatcctg tcaatgatgc acgtgagctt gcccaggtct
     1441 atggtgttga gcaccgggtg tacgggcacg tggacgaccc cctgctcgcc tatgcccagg
     1501 atggtgcccg ccttgcagat ggagtagtga ccccgcttcg agaccaggaa cagggcctgg
     1561 cggtagccgt agtgcgcgag ggcgcgggcc aacccctcga ccctcacccc cctgaatccc
     1621 ttgcggtcgg gcgggaaggc cttggccatg gccagcgtga gcgccgtgag gttcgccatg
     1681 gtgccgtccg atgtgatgtt gcccagggcc acctcggggt tctggatgtg ccggtggtag
     1741 aactcgggcg ggttgtcgta gatgaggtgg tggatccagc acaagaactc cctctccacg
     1801 aaggtggagg cctttgcgct ctctatcttc acctggttct ggttgaggct gacggcgatc
     1861 atctcgagga ggatcatgaa gtaggggatc gcgctcgtca tgtggccgat gtagtagggg
     1921 ttcgccacct tcaccgagtg cctgatgatg ttgttcttga tctcgtcgag cacgtccttg
     1981 atcaggcggg gctcgcccgg catctcgatg ttggagaaga tcccggcgag ttcctccatg
     2041 gagatggacg agtggatccc gcccttttcc ttgaagaagt cgtggatcat gtccaggagg
     2101 tgtaccccga attccacgaa tcggtcctgg gaatcgggca tgatgaagag cttccgcatg
     2161 tactccggag tgctctcgat ccgcctgtcg tggtgcttgc cgtctagctt caaggattcc
     2221 atgcgcggac ctctgtcttc cctttacggt atccatcgta tataccataa gcacaaaggg
     2281 ccccaaaagg agaaaactgc ccggtgcaag gcgctcgacc agccctttac gtggtcccga
     2341 caccgctcgg taacctcaag gacgtcacgc tccgcgttct cgatgtgctc gcagccgtgg
     2401 acctcgtcct ctgcgaggac acgcgccgca cccggaagct cctctcccac taccggctgt
     2461 cgaagcagct ggtctcctgc gagcggttca gcgaggcccg aagggtgtcg tacgtcctgg
     2521 gggcgctcgg cgaggggaag agcctggcgc tcgtcagcga cgccggcatg ccggggattt
     2581 ccgaccctgg aacgcgcatc atccagagtg tccgcaggga gggatacgag gtcgtggtcc
     2641 tccccgggcc ttcggcgatc gtcaccgcgt ttgcagccag cgggatcgac aagcccttca
     2701 ggttcatcgg tttcctgccc aggaagacag aggaaaagag gctcgccgtg catgccatgc
     2761 tctcgtcgag ggacgcaacg gtgttctacg agtccccgag gaggctgctg tcgacgctca
     2821 aggaactggc cgcaggcgac cctggcaggt tcgtctgcat cgcgagggag atgacgaaga
     2881 tccacgagga atacctcgcc ggaagcgccc gggaggtgct gtcacaactg gagggaaagg
     2941 acgtcagggg cgagctcacg gtggtggtgg acgggtcgtc tcgagacgcc gaaccctctt
     3001 gggacgcgat cgaccgcgcg ggtatgaggc tgctggcttt gggggtgccc ctgaaggagg
     3061 cgtccgccgt gctggccgat gtctccggcc tctccaggac ggagctctac aggcgcctgc
     3121 tcgcttcctg cagaaaaggg ccgtagggat ggggaaccaa agatcgcggc tcgaaagcgt
     3181 cctgacggcg gtccttgctc accgggggcg cgatgggccc ggcccgaggc gctccgggaa
     3241 tcccgggcag ggtatgcggc agcatccggg cgggtcctcc gacctttcac cttgccggtt
     3301 tcgagcgcgt gcgcctcgcc caggaacagg ccgccggggg cgagcggtgg acgggaaggg
     3361 ccgagcgcgt gcagcgctct cccacgagca cccgtgcttg tgaggtcggc ccacgtcagg
     3421 ccagggcggg aggctctccg accctctgaa agcccaggcg gtgacttcgg cgatcccttg
     3481 cgcaaaacga gcacgcatgc cgtgcctgcc aggcaagccg ccggtcttgc gggccccgta
     3541 gcgcccggcg gtgacagtgc ctacctgatg cgcacggtcg ccagcagagg atgcgactcg
     3601 accacgaacg tggacacgtc cgggttcgtc ttgagcatgt tgcaggc
//
LOCUS       DAOSCR010000240         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16220_length_3636_cov_3.344876, whole genome shotgun sequence.
ACCESSION   DAOSCR010000240 DAOSCR010000000
VERSION     DAOSCR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16220_length_3636_cov_3.344876"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLT69_06870"
     CDS             <1..684
                     /locus_tag="PLT69_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ14194.1"
                     /translation="VCGLFHDLGKIEIPKGILKKQGALDKDELDTMRAHPLLGVKKII
                     RLNAPRVLRSRIILGPFEHHLNPDMTGYPRTHFMKGVSVMGKILKIVDVYDALTSERV
                     YRKRAFTPEEALRKMWAQAGKSFDPILLKCFVSMMGIYPVGSVVELRGGALGIVSMYS
                     PGALRDRPVVMVLEHDGSGGYVCAGEIDLAKEPLEDGDAGQAIARGIPAPMLGINPSA
                     FLLPGLSPA"
     gene            1379..2116
                     /locus_tag="PLT69_06875"
     CDS             1379..2116
                     /locus_tag="PLT69_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOJ14195.1"
                     /translation="MDILKPLKAESLVEVFVSRFEELILTGKLAIGQRLPSERELALM
                     LGVSRPVVHEGLVDLEAKGLVTMRPRVGTVVNDYRKEGSLAILNSIINFHKGALDESV
                     LSGLLAVRELVESETARLAAVHRTQAQLEELEEIVQKERAADPKDVESVTRLDFAFHH
                     TIAMCSGNIIYPLLINSFKTVYMNFTGMFFSDESMKPAIVGFHELIVDAVAAKDPERA
                     ASIMRDMLAHGELNLRRMLKQGEGRRP"
     gene            2113..>3636
                     /locus_tag="PLT69_06880"
     CDS             2113..>3636
                     /locus_tag="PLT69_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ14196.1"
                     /translation="MSTAQACVAQKRLKQPKNLSPRIEWLRRYYLEGAARRWNNEFTA
                     WTTGTPWDEVFDETTFYIVPETYPFFQTFRSSFRQAARKVDLHPDFWTWSIAERRAWF
                     VKEVMVNRLPQEILPGDLLCGARFNIQTSLCLDERETKRRNRLMFGKSGVRRAVKWFH
                     DHGYGNSGATSGHLIPGYERILKEGFKAVHDDLEARLQALDPREHNGEKGAQLRAMKI
                     ASTMPRELASRYAELCERTAAGEPDPVRKAELEQMGRNLRRVPWEPAGTFWEAVQALW
                     LTHMLVMSDENYPGPGVSFGRIDQYLLPYWRASIEQGMDREFGKEILKCFWVHANTAY
                     DAMVRIGNNGITAGYGQLITLSGMGQGGRDMTNELTFAILEVIDEMSPILEPKPNVRL
                     HRNSPDELLDKVVEMIASSQGSPFLLNFDERSMAGMMVEARKAGVTHLIHEGNVHEYA
                     PVGCLENTMVGNDRSGTVDNNLNLLKAVELALNDGRDFFDFVDPITLEKEKARQDGPR
                     "
BASE COUNT          725 a         1153 c         1176 g          582 t
ORIGIN      
        1 gtttgcgggc tcttccacga cctcggaaag atcgagatcc ccaaggggat actcaagaag
       61 caaggcgctc tggacaaaga cgagctggac accatgcgcg cgcacccgct cttgggggtg
      121 aagaagatca tcaggctgaa tgcgcccagg gtcctgaggt cgaggatcat cctcgggccc
      181 ttcgagcacc acctgaaccc tgacatgacc ggctacccca ggacccactt catgaagggc
      241 gtcagcgtca tgggcaagat cctcaagatc gtggacgtgt acgacgccct tacctctgag
      301 agggtgtacc gaaaacgcgc cttcaccccg gaagaggcgc tgaggaagat gtgggcccag
      361 gcgggcaaga gcttcgaccc catcctgctc aagtgtttcg tgtccatgat gggaatatac
      421 ccggtcggtt ccgtcgtcga gctcagggga ggcgccttgg gcatcgtcag catgtactcc
      481 cccggcgccc tgagggacag gccggtggtc atggtcctcg agcacgacgg gtccgggggg
      541 tacgtctgcg cgggcgagat cgacctggcc aaggaacctc tcgaggatgg cgacgcagga
      601 caggccattg caagggggat acccgcgccg atgctcggta tcaacccgtc cgcctttctc
      661 ctccccggcc tgagccccgc ctgaggagtc atccagtcgg gccgtgcagc cgcgcgcggc
      721 cgccccatgc gcacatgagg ggtgggatcg agtctggaca cgtgctggac cgcgccgcag
      781 cgatcatctc gaaagcatgg ggaaacaggc ccatctctag gcgccacgca ccctccacac
      841 ccgtcacgag cgtttggggc cccttggcgg atgcggctgg caggcagggc tggtggacca
      901 tgaccgcacg tgtcaccggg ggccgcgccc gcacggcacg tcgagagcga ctctatccgc
      961 agttgcgttc tccccgaacg tgtcgcagag ggggcctttc cacggagatg cggtgaggtg
     1021 tatccgggcc tcggccccgt cccgcgacgg gaagacacga gggcgagcca gccgagaaga
     1081 tgacccggcc gttgcctttt cccggcgcca cgagaccccc gggcgccatc acgacgtctc
     1141 ttcccatcgg ccacttccgt gcttgacaca ctccttcccc cgtctcgttc ccgatgcccg
     1201 tcccgcccgt cgcccctgcc ccggggacgt cgcagcccct gatcgacggt taccttgagt
     1261 ggtgaccccg aagtggcgcc ctagtgacga aggccccgca accttcccct tgcgcagggg
     1321 gtggatccgg ggtatattgt cagctggaca taccaccagc cggaaaggac gcgacatatt
     1381 ggacatcctc aagccactga aagcggagag cctggtcgag gtcttcgtga gcaggttcga
     1441 ggaactcatc ctcacgggaa agctggccat cggccaaagg ctgccttccg agcgcgagct
     1501 cgcgctcatg ctcggagtga gcaggccggt ggtgcacgag gggcttgtcg acctcgaggc
     1561 aaagggactc gtgacgatga ggccccgggt gggcacggtc gtgaacgatt accgcaagga
     1621 ggggtctctt gccatcctga actccatcat caacttccac aagggcgcac ttgacgagtc
     1681 cgtgctgtcg gggcttctcg ccgtcaggga gctggtggag tctgagacgg cgcggcttgc
     1741 cgccgtccac cggacccagg cccagctcga agagctcgag gagatcgtgc agaaggagag
     1801 ggccgcggac ccgaaagacg tggagagcgt gaccaggctg gacttcgcct tccaccacac
     1861 catcgccatg tgctcgggaa acatcatcta cccgctgctc atcaactctt tcaagacggt
     1921 ctacatgaac ttcacgggca tgttcttctc ggacgagtcg atgaagcccg ctatcgtcgg
     1981 gttccacgag ctgatcgtgg atgcggtggc ggcgaaagac ccggaaaggg ccgcttccat
     2041 catgcgtgac atgctcgccc acggcgagct gaacctgcgc aggatgctga aacaggggga
     2101 agggaggcgc ccatgagcac ggcacaggct tgcgtggctc agaagaggct gaaacaaccc
     2161 aagaacctct cgccccgcat cgagtggctg aggcggtatt atctcgaggg tgcggcaagg
     2221 aggtggaaca acgagttcac ggcgtggacc accggcacgc cttgggacga ggtcttcgac
     2281 gagacgacct tctacatcgt ccccgagacc taccccttct tccagacctt caggtcgtcg
     2341 ttcaggcagg cggcccgcaa ggtggacctc catcccgact tctggacctg gagcatagcc
     2401 gagaggaggg cctggttcgt caaggaggtc atggtgaacc ggctccccca ggagatcctc
     2461 cccggtgacc ttctctgcgg ggcgaggttc aacatccaga cctcgctctg cctcgatgaa
     2521 agggagacga agcggaggaa caggctcatg ttcgggaaaa gcggcgtaag gagggctgtg
     2581 aagtggttcc acgatcacgg ctacgggaac tcgggggcaa cgagcggcca cctcataccc
     2641 gggtatgagc gcatcctgaa ggaggggttc aaggccgtcc acgacgatct cgaggcccgc
     2701 ttgcaggccc tggacccgcg tgagcacaac ggagagaaag gcgcacagct gagggccatg
     2761 aagattgcat cgaccatgcc cagggagctt gcatcccgct acgccgaact ctgcgagcgg
     2821 accgccgccg gggagcccga ccccgtgcgc aaggcggagc tcgagcagat ggggaggaac
     2881 ctgaggcgcg tcccctggga accggccggg accttctggg aggcggtcca ggccctgtgg
     2941 ctcacgcaca tgctcgtcat gagcgatgag aactaccccg gccccggcgt ttccttcggg
     3001 cgcatagacc agtacctcct cccctactgg agggcctcca tcgagcaggg gatggaccgc
     3061 gagttcggca aggagatcct caagtgtttc tgggtgcatg cgaacacggc gtacgacgcc
     3121 atggtccgaa tcggcaacaa cggcatcacc gcgggctacg gccagctcat aaccctctcc
     3181 ggcatgggcc agggcgggcg cgacatgacg aacgagctca ccttcgcgat cctcgaggtc
     3241 atcgacgaga tgagccccat cctcgagccc aagccgaacg tgaggctgca caggaacagc
     3301 cctgacgaac tgctggacaa ggtcgtggag atgatcgcct cgagccaggg ttccccgttc
     3361 cttctcaact tcgacgagcg gtccatggcg gggatgatgg tggaggcgag gaaggccggc
     3421 gtgacgcacc tcatacacga ggggaacgtc cacgagtacg cgccggtggg atgcctcgag
     3481 aacaccatgg tgggcaacga ccgctcggga acggtggaca acaacctcaa cctcctcaag
     3541 gccgtcgagc tcgcgctcaa cgacggcagg gacttcttcg acttcgtcga ccccataacc
     3601 ctcgagaagg agaaggcacg acaggacggc ccaagg
//
LOCUS       DAOSCR010000241         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16242_length_3632_cov_3.789768, whole genome shotgun sequence.
ACCESSION   DAOSCR010000241 DAOSCR010000000
VERSION     DAOSCR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_3632_cov_3.789768"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..754)
                     /gene="mobB"
                     /locus_tag="PLT69_06885"
     CDS             complement(218..754)
                     /gene="mobB"
                     /locus_tag="PLT69_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOJ14197.1"
                     /translation="MAFEIDHGKFLSFVAQGTNSGKTTILEGVIAELKRRGRRVCALK
                     HGMHMHYHDPAGKDTERFASSGADRVAMFSPEGLLVYENVTPSLEYLMLAASHGVDVV
                     LVEGYKSGPFRKIEVFNDTLYERPLCVEHPSPDYIALVCNRAIPLDIPTFLFGQIEEI
                     TTFVEESVGLGRHAASNP"
     gene            857..1153
                     /locus_tag="PLT69_06890"
     CDS             857..1153
                     /locus_tag="PLT69_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14198.1"
                     /translation="MAADVPLLEALRWAAIVFVAGFIGFFGKYLARLVLDRIHARAKG
                     PGDLPRDRSARKDAPGESPPPVTGAGADAPAPRATDPKVMKKLLKARAKAIKKS"
     gene            complement(1160..1606)
                     /gene="fliW"
                     /locus_tag="PLT69_06895"
     CDS             complement(1160..1606)
                     /gene="fliW"
                     /locus_tag="PLT69_06895"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOJ14199.1"
                     /translation="MEEIDTLRFGRIKVEGDKIITFPRGILGFSRCRRYALFPHGEKS
                     PFCWLQSVEDAALAFVVMDPALVDPRYQVDVDEQTARELDIAPGDKIQALCIVTIPQG
                     DPRRMTVNLLGPLVVNTRNRLAAQVICENRGYSHRRPLLAEEREAP"
     gene            complement(1628..2716)
                     /gene="flgL"
                     /locus_tag="PLT69_06900"
     CDS             complement(1628..2716)
                     /gene="flgL"
                     /locus_tag="PLT69_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOJ14200.1"
                     /translation="MKVSNRFLYYQLVKDLNQNTERLFKLNNQISSGRRISTPSDDPL
                     GLSSVLVYRTELNSFDQYKKSVDYGKGWLNRMDSILQDVDDLLGRVSELAVQAASSTS
                     TQSTRVGAAEEIEEIRSMLLGHANAKYGNKYIFGGTMTQTVPFLSVDVDDLEEDVATM
                     SAAPPASPSEGDRYIDTDDGHIYQYTSGAWNDLGAPAESRSVIVTDTDTVYVFSQGSW
                     RPMYQGNDETFSFAIGKENSAQINIPGSRVFLNPDGNIFTSLLRLERALRSNDVNGIQ
                     TEMGVIERAQTVISDNLAAIGAVENKLDHTLTVIDRSRVDTKSMVSSIEDLDYAQAIT
                     ELKNQETIYEAALKSASMITSLSLVDFI"
     gene            complement(3029..>3632)
                     /locus_tag="PLT69_06905"
     CDS             complement(3029..>3632)
                     /locus_tag="PLT69_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ14201.1"
                     /translation="PVRFLDSLGCLGRRTLAVHCVVLDDEDIRILAERSVSVSHNAES
                     NMKLASGVSPVPKLLAAGVNVSVGTDGCASNNNLDLLGEISTVSKLHKITSMDPTVIP
                     DRTALSMVTLSGARALGYDGGVLGPGRPADITVLDGSSPNLVPVHNPVSHVVYAATGG
                     NVKDVVIGGRVVVRDAKCVTIDEEALLRECRDIAKRIRKA"
BASE COUNT          600 a         1163 c         1175 g          694 t
ORIGIN      
        1 tctcctccga ggaagaccga ccggccttct tggcgcgcgg tcaggggtct tgtcgctcac
       61 accgggagac gccgacgttc acccgggggc ccgcttaaac gacgtctgcg acacacaccg
      121 agatccgggc ctgggcctgc aatgcccttg ccagccgccg gcaggccacg ggggcgcacc
      181 cgcgtggcgc ctcaggcaag gcaggcggtc actcgggcta cgggttcgaa gccgcatgcc
      241 gcccgagacc cacggactct tccacgaacg tggttatctc ctctatctgg ccgaagagga
      301 aggtggggat gtcgaggggg atggccctgt tgcagacgag ggctatgtag tcgggcgacg
      361 ggtgctccac gcagagaggc cgttcgtaca gcgtgtcgtt gaagacctct atcttcctga
      421 acggcccgct cttgtacccc tccacgagca ccacgtcgac gccgtgggag gcggcaagca
      481 tcaggtactc gagggaaggg gtgacgttct cgtacacgag aaggccctcg ggggagaaca
      541 tggccacccg gtccgcaccg ctggatgcga acctttcggt gtccttgccc gcggggtcgt
      601 ggtagtgcat gtgcatcccg tgcttgaggg cgcacaccct cctgcctctg cgcttgagct
      661 cggcgatgac cccttcgaga atcgtggtct tgccgctgtt cgtcccctgg gcgacgaaag
      721 agaggaactt cccgtggtcg atctcaaagg ccatggtgtg tgtatacgtg ccctcttgaa
      781 tgccggtcaa gggcttgtgt cacgcaacct cacgagaccg gttgccccga agacggccat
      841 ggtctaagat catcccatgg ctgcggacgt tccccttctc gaggcgctca gatgggcggc
      901 gatcgtcttc gtcgcggggt tcatcggctt cttcggcaag tacctcgcga ggctcgtgct
      961 cgacaggata cacgcccgcg cgaaaggccc cggagacctt ccccgcgatc ggtccgcccg
     1021 taaagacgct cccggcgaat ccccaccgcc cgtcaccggc gccggggcgg acgcacccgc
     1081 acctcgggcg accgatccca aggtgatgaa gaagctcctc aaggccaggg cgaaggcgat
     1141 caagaagtct tgaggacgat cagggggcct ctctctcctc ggccaagaga ggccttctgt
     1201 gggaataccc ccggttctcg cagatgacct gggccgccag cctgttcctc gtgttgacca
     1261 ccaggggtcc gaggaggttg acggtcatgc gcctcgggtc accctggggg atggtcacga
     1321 tgcagagcgc ctggatcttg tctccgggag cgatgtcgag ttccctggcc gtctgctcgt
     1381 ccacgtcgac ctggtagcgg ggatccacca gggcggggtc catgaccacg aaggcgagcg
     1441 ccgcgtcctc cacgctctgg agccagcaga agggcgattt ctcgccgtgg ggaaagagcg
     1501 cgtaccgcct gcaccgggaa aagccgagga tcccccgtgg gaaggtgatg atcttgtccc
     1561 cttcgacctt gatcctgccg aaccggaggg tgtcaatctc ttccaatgat cgtccccctt
     1621 cttctcatca gatgaaatcc acgagactca ggctcgtgat catcgaggcg ctcttcaggg
     1681 cggcctcgta tatcgtctcc tggttcttga gctcggtgat ggcctgcgcg tagtcgagat
     1741 cctctatgga cgagaccatg ctcttggtgt ccaccctcga gcgatcgatc acggtcaggg
     1801 tgtggtcgag cttgttctcc accgcaccga tggccgcgag gttgtcggag atgaccgtct
     1861 gcgccctttc aatgacgccc atctcggtct gtatcccgtt cacgtcgttc gatctcaagg
     1921 cgcgctcgag cctgaggagc gacgtgaaga tgttcccgtc cgggttcagg aagacccggc
     1981 tcccggggat gttgatctgc gcggagttct ccttgcctat ggcgaaggag aaggtctcgt
     2041 cgttgccctg gtacatgggc ctccacgacc cttgggagaa gacgtagacc gtatcggtgt
     2101 ccgtgacgat gaccgacctc gactccgccg gcgcgccgag gtcgttccac gcacccgagg
     2161 tgtactggta gatgtgcccg tcgtcggtgt ctatgtagcg gtctccctcc gagggcgatg
     2221 cggggggagc ggcagacatg gtcgcgacgt cttcctccag gtcgtccacg tcgacggaca
     2281 ggaaggggac ggtctgggtc atggtgccgc cgaagatgta cttgttgccg tacttcgcgt
     2341 tcgcgtgccc caggagcatc gacctgatct cctctatctc ctcggcggcg cccacccggg
     2401 tgctctgcgt cgaggtggag gacgccgcct ggaccgcgag ttccgagacc ctgcccaaga
     2461 ggtcgtccac gtcctggagg atggagtcca tcctgttgag ccatcccttg ccgtagtcca
     2521 cggacttctt gtactggtcg aaggaattga gctcggtgcg gtacaccagg acgctcgaca
     2581 ggcccagggg atcgtccgag ggcgtgctga tcctccgtcc cgaggagatc tggttgttca
     2641 gcttgaacag cctctcggtg ttctggttca gatccttcac gagctggtag tagagaaacc
     2701 ggttgctcac cttcatattc cacctctatc ggcacatggc cgcgctgtct tgatggaaag
     2761 caaggatgct gccaggggga tggatcctcg agccggatac ggaacgcccg ggaacggaca
     2821 ggcaaaccct tccccccatg gggcatcctt ttccccggtc atggccgggc caggctgcgg
     2881 ccgacgtccg cgacggcccg cccggcgcac acggggcccc gccgggatgc atcgcagggc
     2941 cctgcatgca ggccaaaggc gggtcgagac catccaaggc ccttgggcca cgttccccac
     3001 cttgggcatc gagatacgcc cgcgttcctc aggccttcct gatgcgcttg gctatgtctc
     3061 ggcactcccg caggagggct tcctcgtcta tggtgacgca cttcgcgtcc ctcaccacca
     3121 cccttccccc gatgacgacg tccttcacgt ttccgccggt tgccgcgtac accacgtggg
     3181 agacgggatt gtgcaccggg acgaggttcg ggctggagcc gtcgaggacg gtgatgtcgg
     3241 ccggcctgcc cgggcccagg acaccgccgt cataccccag ggcacgggcg cccgagagcg
     3301 tgaccatgga aagcgccgtc cggtcgggga tgacggtggg gtccatggag gtgatcttgt
     3361 gcagcttgga taccgtcgag atctcgccca agaggtcgag gttgttgttg ctcgcgcatc
     3421 cgtccgtgcc caccgacacg ttcacccccg cggcgaggag cttggggaca ggggagacgc
     3481 cgcttgcgag cttcatgttg ctctcggcgt tgtgcgagac cgacacggag cgctcggcga
     3541 ggatccttat gtcctcgtcg tcgagcacca cgcagtgcac cgcgagcgtg cgcctgccga
     3601 ggcaaccgag cgagtcgagg aacctcacgg gg
//
LOCUS       DAOSCR010000242         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16283_length_3626_cov_3.494259, whole genome shotgun sequence.
ACCESSION   DAOSCR010000242 DAOSCR010000000
VERSION     DAOSCR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16283_length_3626_cov_3.494259"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLT69_06910"
     CDS             <1..200
                     /locus_tag="PLT69_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14202.1"
                     /translation="AKKAAVPGKLAHRKSAVLGALIAALVVLVLLVASLRYCTRHEGR
                     GPADGPAGGVSSLVESGTAPS"
     gene            244..948
                     /gene="recO"
                     /locus_tag="PLT69_06915"
     CDS             244..948
                     /gene="recO"
                     /locus_tag="PLT69_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ14203.1"
                     /translation="MPHYTSEAIILSASDFMESDRIVHALTREQGLVCAIAKGAHKSR
                     RRFPGALEPFSEVLMHVHRGRGSGLQRIEQARLLNAHMPLRQDIHLMGYACVLLEIAK
                     ENLGQQDPAPEVYACLKNALDGLSGSTRWFNLWCTSLMDMLRHIGYGLRPDSFAPAGM
                     QEGLASSLSVGPEARSFMERAPGLTGEVLSRISLSARAKTEITLFLLDVCSRVTPRPL
                     STAVFLAKLLDLDMKQ"
     gene            962..1822
                     /locus_tag="PLT69_06920"
     CDS             962..1822
                     /locus_tag="PLT69_06920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOJ14204.1"
                     /translation="MDFQELILRLHEFWSERGCLIAQPYDLEVGAGTFNPATFLRALG
                     PDPWRVAYVEPSRRPTDGRYGENPNRLQHYYQYQVILKPSPLDVQEIYLDSLRRLGID
                     TLEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQVGGFDLDPVCTEITYG
                     LERIAMYLQNKDSVFDIQWKDRWTYGYVHHEGEVQWSKYNFELADIAMLLELFNLFEK
                     EGKRIAEKGLCLPVYDMCLKCSHIFNLLNARGAISTTERQSYVLRVRELARLAAKLYL
                     DSAAAGGGEG"
     gene            1822..>3626
                     /gene="glyS"
                     /locus_tag="PLT69_06925"
     CDS             1822..>3626
                     /gene="glyS"
                     /locus_tag="PLT69_06925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOJ14205.1"
                     /translation="MTETLLFEIGTEEIPSAFIDPAVEFMRAYLAKALDGLRLSHGEM
                     KAFSTPRRLAILVRDVPDTMPDTVETKMGPPKAVAFDKDGKPTKAGLGFAANCGVDIG
                     DVTVTTTPKGEYLCVTKTTPGRPAREVLPGMLEDMIQKIPFPKTMRWSNPGVRFARPV
                     HWILAIFGAEVLPVSFGSVSSGRFTYGNRFMANVPIAVDHPDAYEETLEKAYVIADPA
                     RRKRIVWESLERSAAAVGAVVKDEDLVEEVANLLEYPHPIVGTFDEGYLELPKEVLVT
                     VMKHHQRYFPMYDANDGSRIKNAFGTFSNIVPKSDDVVRSGNERVLKARLDDGKYFFS
                     EDLKVPLSVYADRLREVIFHKDLGTSWEKVERFTRVALALAERLAPEKKEQVREAAYL
                     CKADLNSLMVGELPELQGIMGREYAVRQGIDPEVAAAIREHYLPASADDVLPSGILGD
                     IVGMADRIDTICGCFGVGMVPTGTSDPYALRRQTIAIENVLLGKGYRVSVGWLVDVAL
                     EQLAGKLKRDVSEVKAEVVAFFANRFASILQSRGIEGDVIESVLTTFDDPVDTFMRAQ
                     ALSEVLGEPWFVSICAASKRVENILKKVRATGTVT"
BASE COUNT          677 a         1137 c         1189 g          623 t
ORIGIN      
        1 aggcgaagaa ggcggctgtc cccggcaagc tcgctcaccg gaagtcggcg gtgctcggcg
       61 ccctcatcgc ggccctcgtt gtgctggtcc tcctggtggc ctccctcagg tattgcacgc
      121 ggcatgaggg tcgagggcct gccgacggcc ccgccggggg cgtgtcttct cttgtcgaga
      181 gcggcacggc cccgtcctga cagaaaatcc ctggaaggcc cggatccccg ggcagagcct
      241 ggcatgcccc actacacgag cgaggctatc atcttaagcg cttctgattt catggagtcg
      301 gacaggatcg tgcacgccct gacccgggag cagggcctcg tgtgcgccat agcgaagggc
      361 gcccacaaga gccggagacg cttcccgggg gcgctggaac ccttcagcga ggtgctgatg
      421 cacgtgcacc ggggaagggg ttccggcctc cagcgcatcg agcaggcccg gcttttgaac
      481 gcccacatgc ccttgaggca ggatatccac ctcatggggt atgcttgcgt gctcctcgag
      541 atcgcgaagg agaacctcgg ccagcaggac ccggcgcccg aggtctacgc gtgcctcaag
      601 aatgcgctcg acgggctttc gggctcgacc aggtggttca acctctggtg tacctccctg
      661 atggacatgc tcaggcacat agggtacggg ctgaggcccg attccttcgc gccggcagga
      721 atgcaggagg gcctcgcctc ttcgctctcg gtcggccccg aggccaggtc gtttatggag
      781 cgggcgcccg gcctcaccgg ggaggtgctt tcgaggatca gcctgagcgc ccgggcgaag
      841 accgagatca cgctgttcct tctggacgtg tgctcgaggg tgacgccgag gccgctctcc
      901 acagccgttt tcctggcgaa gctgcttgac ctcgatatga agcaatgata caggaaaggc
      961 catggatttt caggagttga ttttgcgttt gcacgagttc tggtccgaga ggggctgtct
     1021 catcgctcag ccttacgacc tggaggtggg ggcggggacc ttcaacccgg ccaccttcct
     1081 cagggcgctg gggcccgacc cctggagggt ggcgtacgtg gaaccctcga gacgccccac
     1141 ggacgggcgg tacggggaga acccgaaccg cctccagcac tactaccagt accaggtgat
     1201 cctcaagccg tcgcccctcg acgtccagga gatctacctc gacagtctca ggcgcttggg
     1261 gatcgatacc ctggagcacg acatcaggtt cgtcgaggat gattgggaat cccccaccct
     1321 cggcgcctgg gggctgggat gggaggtctg gctcgacggc atggagatca cccagttcac
     1381 ctacttccag caggtgggcg gcttcgacct cgaccccgtg tgcaccgaga tcacttacgg
     1441 cctggagagg atagccatgt atctccagaa caaggactcg gtgttcgaca tccagtggaa
     1501 ggaccgttgg acctacggtt acgtgcacca cgagggcgag gtgcagtggt cgaagtacaa
     1561 cttcgagctg gcggacatcg ccatgctcct cgaactcttc aacctcttcg agaaagaggg
     1621 caagcgtata gccgagaagg ggctctgtct gccggtctac gatatgtgcc tgaaatgctc
     1681 acacatcttc aacctcctca acgcgagagg tgcgatatcg accacggagc gccagtccta
     1741 cgtcctcagg gtcagggagc tggcaaggct tgcggccaag ctctacctcg actcggcggc
     1801 cgcaggcggg ggggagggct gatgaccgag accctgctct tcgagatcgg gacggaagag
     1861 atcccgagcg ccttcatcga cccggcggtg gagttcatga gggcgtacct ggccaaggcc
     1921 ctcgacggcc tgaggctctc ccacggcgag atgaaggcgt tctccacccc gaggaggctc
     1981 gccatcctgg tgagggacgt gccggacacc atgccggaca ccgtggagac gaagatgggt
     2041 ccgcccaagg cggtggcctt cgacaaggac gggaagccca cgaaggccgg cctgggattc
     2101 gccgcaaact gcggggtgga catcggcgat gtcaccgtga ccacgacccc caagggggaa
     2161 tacctctgcg tcacgaagac gacccctggg cggcccgccc gcgaggtgct gcccggcatg
     2221 ctcgaggaca tgatccagaa gatccccttc ccgaagacca tgcggtggtc gaacccgggc
     2281 gtgcgcttcg ccaggcccgt tcactggatc ctcgcgatct tcggggcgga ggtcctgccc
     2341 gtctccttcg gatcggtctc gtcgggacgc ttcacctacg ggaaccgctt catggcgaac
     2401 gttcccatcg ccgtcgacca cccagacgct tacgaggaga ccctcgagaa ggcctacgtg
     2461 atagccgatc ccgcaaggcg caagaggatc gtctgggagt ccctcgagcg gagcgccgcc
     2521 gccgtcgggg ccgtcgtgaa ggacgaagat ctcgtggaag aggtcgcaaa cctcctcgaa
     2581 tacccgcacc ccatcgtggg gaccttcgac gaggggtacc tcgagctccc gaaagaggtg
     2641 ctcgtcacgg tcatgaagca ccaccagcgc tacttcccca tgtacgacgc gaacgacggc
     2701 tcgaggatca agaatgcgtt cgggaccttc agcaacatcg tccccaagag cgacgatgtg
     2761 gtccgctcgg gcaacgagcg tgttctcaag gcccgcctgg acgacggaaa gtacttcttc
     2821 tccgaggacc tcaaggtccc cttgagcgta tatgccgacc gtctcaggga ggtgatcttc
     2881 cacaaggacc tcggcacctc gtgggaaaag gtggagcggt tcacgagggt cgccctggcc
     2941 ttggccgaaa gactggctcc cgagaagaag gagcaggtga gagaggctgc gtacctgtgc
     3001 aaggccgacc tcaacagcct catggtgggc gagctgcccg agctccaggg gatcatgggg
     3061 cgggagtacg ccgtccgcca ggggatcgac cctgaggtgg ccgcagcgat cagggaacac
     3121 taccttcccg catccgcgga cgacgtcctg ccctcgggca tcctcgggga catcgtgggc
     3181 atggcggacc ggatcgacac catatgcggc tgtttcggcg tcggcatggt ccccaccggg
     3241 acgtctgacc cctatgcctt gcgcaggcag accatagcca tcgaaaacgt gctgttgggc
     3301 aaggggtacc gggtgtccgt cgggtggctg gtggatgtcg ccctggagca gctggccggc
     3361 aagctcaaga gggacgtgtc ggaagtcaag gccgaggtcg tcgccttctt cgccaacagg
     3421 ttcgcttcga tcctccagtc tcgggggatc gagggagacg tcatagaatc agtcctcacc
     3481 acgttcgacg accccgtgga caccttcatg agggcgcagg cgctctccga ggtgctcggc
     3541 gagccctggt tcgtctctat atgtgcagcg agcaagcggg tggagaacat cctcaagaag
     3601 gtgcgggcca ccgggaccgt caccga
//
LOCUS       DAOSCR010000243         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16284_length_3626_cov_3.297116, whole genome shotgun sequence.
ACCESSION   DAOSCR010000243 DAOSCR010000000
VERSION     DAOSCR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16284_length_3626_cov_3.297116"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLT69_06930"
     CDS             <1..299
                     /locus_tag="PLT69_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ14206.1"
                     /translation="AGYAIVNLVFGNIVEPIFMGRGLSLSTLVVFLSLIFWGWVLGPI
                     GMLLSAPLTMVFKIAFDSFPSTKWISVLLDASVPLPIDLPHKGSDTKTGSSRNA"
     gene            458..1147
                     /locus_tag="PLT69_06935"
     CDS             458..1147
                     /locus_tag="PLT69_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOJ14207.1"
                     /translation="MRRILCLVLVVLAATQPRSTFAADDAAASRLRTSLNALSGIMAS
                     PSPIPPSLIQRAKAVVIVPDVLKISFFAGTRFGRGVMVARTEDRQWGNPVFVTLKGGS
                     FGIQAGLQSTDLVLVFLKGTPANSSLREGIFIGADANLVLGNRGIHFDENTSPNLEME
                     VYSFARTSGVTAGFSLNGMQLGLDDALTAHLYGKPGLKASDVLSGRVSIESKDASRFR
                     SAFLRIIGSSP"
     gene            1151..2089
                     /locus_tag="PLT69_06940"
     CDS             1151..2089
                     /locus_tag="PLT69_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ14208.1"
                     /translation="MRSNQARAYAHGLLAVFLWSTVATAFKLSLEYAHPLQLLAYAAL
                     TSTIVLGAYLILTGRTVGITSRPPRLFALSLLQGFLNPFLYYAILFKAYALLPAQIAQ
                     PLNYTWAFTLSALSAVFLKQKIYKRDVVSGLVCYLGVAVLLTGGRFDTFTSFDPLGVF
                     LALASTVVWSVSWILNTRDPRDVVESLFLSFASSLPFTAIACAWVAPPVPDVRAVAGG
                     VYVGLFEMGITFVLWLKALRLSENAAKMANLIFISPFISFMFIHSIVGEPIRASSVAG
                     LVIIVCGLSLQRPRSRDKGRRDGYGARQPPSCPSSS"
     gene            complement(2687..2923)
                     /locus_tag="PLT69_06945"
     CDS             complement(2687..2923)
                     /locus_tag="PLT69_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14209.1"
                     /translation="MKKGTMWVLFVVVVFGLMMFFSGCQKKEQPAEQPAVTEQAAPAD
                     TAAPADTAAPADTAAPADTAAAPADTAAPAGTAQ"
     gene            complement(3228..>3626)
                     /gene="pal"
                     /locus_tag="PLT69_06950"
     CDS             complement(3228..>3626)
                     /gene="pal"
                     /locus_tag="PLT69_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOJ14210.1"
                     /translation="APEGDQGIQVEGAAPPAPQESFVFDKKIYFDFDRFDLTSESIAT
                     LNELAAYLKTNPGLKVTIEGHCDERGTNEYNLALGERRAKAAYDYLVSQGVDGQRLSR
                     VSYGEERPVDAGHNEEAWAKNRRSEFVLGK"
BASE COUNT          625 a         1206 c         1050 g          745 t
ORIGIN      
        1 cggcagggta tgcgatcgtg aacctcgtgt tcggaaacat cgtcgaaccg atcttcatgg
       61 gaagagggct gtcgctgtcg accctggtcg tctttctctc cctcatcttc tggggttggg
      121 tcttggggcc gatcggcatg ctcctgtccg cccccctcac catggtgttc aagatcgcct
      181 tcgacagctt cccgtccacc aagtggatct ccgtcctcct ggacgcctca gtccccctcc
      241 ccatagacct cccgcacaag gggtccgata ccaagacggg gtcctcgaga aacgcttgat
      301 cagccgccgc ggccgccatg ctcccgggcg atcgagggcc ggtgccggcc acgccttccc
      361 gggcggaaag cccattcgct caaaaaggca tggacgcggc ctccattgta gtatagggat
      421 accgcatcga gaccgcgcac cctgggggag tcacttcgtg agacggattc tttgccttgt
      481 cctcgtggtc ttggccgcca cgcagcctcg gagcacgttc gcggccgacg atgcggcggc
      541 atcgaggctc aggacgtcct tgaacgccct ctcgggcatc atggcctccc cgtcccccat
      601 acctccctcc ctgatccaac gggccaaggc cgtcgtgatc gtaccggacg tgctcaagat
      661 cagctttttc gccggcacca ggttcggcag gggcgtgatg gtagcgagga cagaagaccg
      721 acagtggggg aaccccgtgt tcgtcaccct caagggtggg agtttcggca tacaggcggg
      781 cctgcagtcg acggacctcg tgctcgtctt cctgaagggc acaccggcga actcctcgtt
      841 gagggaaggg atcttcatag gcgcggacgc gaacctggta ctcggtaacc gtggtatcca
      901 cttcgacgag aacacctctc ccaacctcga gatggaggtg tactccttcg cccggacctc
      961 gggagtgacc gcagggtttt cactcaacgg catgcagctg ggcctcgacg atgccctgac
     1021 cgcccactta tacggcaagc cggggctgaa ggcctcggac gtgctttccg gcagggtatc
     1081 catcgagagc aaggacgctt cgagattcag gtccgctttc ttgagaatca tcggatcgtc
     1141 accctgaggt atgaggtcga accaggcacg ggcctacgcc cacgggctgc tcgcggtctt
     1201 tctctggtca accgtggcca cggcgttcaa actgtcgctc gaatacgccc atcccctgca
     1261 gctcctcgca tacgccgccc tgacatcgac gatcgtactg ggtgcctacc tgatcctcac
     1321 cggcaggacg gtcggcatca cgtcacgccc gcccaggctt tttgccctgt ccctcctcca
     1381 ggggttcctg aaccccttcc tctactacgc gatcctgttc aaggcgtacg cgctcctgcc
     1441 cgcgcaaatc gctcaacccc tcaactacac gtgggccttc accctgtccg cgctgtccgc
     1501 ggtgttcctg aaacagaaga tctacaagag ggacgtggtc tcgggcctgg tatgctatct
     1561 cggggtggcc gttctcctca ccgggggcag gttcgacacc ttcacctcct tcgacccctt
     1621 gggcgtgttc ctcgcgctcg cgagcaccgt ggtgtggtcc gtgtcatgga tcctgaacac
     1681 gagggacccc cgcgacgtcg tcgagagcct gttcctctcg ttcgcctcga gcctgccttt
     1741 cacggcgatc gcctgtgcgt gggtggctcc acccgtcccg gacgtccggg ccgtggcagg
     1801 aggggtctac gtggggctct tcgagatggg gatcaccttc gtgctctggt tgaaggccct
     1861 gaggctctcg gagaatgcgg cgaagatggc gaacctcatc ttcatctccc ccttcatctc
     1921 gttcatgttc attcactcga tcgtgggtga gcccatacga gcctcgtccg tggccgggct
     1981 cgtcatcatc gtatgcggcc tttcgctcca gcgtccacga tcacgggaca aggggcgtcg
     2041 agacgggtat ggggcacgcc aacccccttc gtgcccctct tcatcctgac ggggttccca
     2101 cggaaggcgt cccgttctct tggttctcga gcgcctgtgt tccctccaag gcgatatgcg
     2161 caagaaggtt cccgtcaaga gcacacaacc ttcccacaag ccctgggcca tgaaccaaac
     2221 cgcgggcatg gtcgttccct gacagggaac cggcggctgc gtgccgcaag agggttgacg
     2281 gagacgcagg atgcgaacct gtgggtcctc gaacatggac cgccatgccg gtcacgatca
     2341 gtgatgcgcc ggggcctttt tccccgaatg ccccccatca ccacgtctga ccgaccggag
     2401 gataccctcg ggatgtcttc ctccacacgc cggaccttta cgcatgggga gacggactgg
     2461 aagaacacct cgtgcactgc acgcgaatgc ccgccgtggg aatctccgtc gagccacgcc
     2521 gacctggaga cagaggggag gctccggcgc atctgggtgt caaggtgtgg aaatccctgc
     2581 cgagccacgc cgatccggag accgcggcaa ggcggacaag aaaaagtcca cccccggcgc
     2641 tcggccgggg atggaccctg tcatgctgct tccgtgtaac ccttgcttac tgggcggtgc
     2701 cagcaggagc cgcggtgtcg gcaggagccg ccgcggtgtc agccggggcc gcggtgtcag
     2761 caggagccgc ggtgtcagcc ggggccgcgg tgtcggcagg agcggcctgc tcggtcacag
     2821 ccggctgctc ggcgggctgt tccttcttct ggcacccgga gaagaacatc atgagcccaa
     2881 acaccacaac cacaaacaac acccacattg tacccttctt catagtctca tctcctatcc
     2941 tttttttttc tgccgattgg tttcagcagg cggtatccta tctcctccct cactcagagc
     3001 accttatatc gtcatcgccc agatatcggc gggaatgcct gtggtttggg gacgtccacc
     3061 gagaaacgtt gcctgcaatg ccacacggag cttcgagaaa gaaactcccg tgaagagcga
     3121 aaaggattgt ctcttcgtga ctgcgctcgt tcttcaccga ttcgctatcc accatggtga
     3181 ttccttccat agaaccactc gtgcgacctt gtcaataccc tttctgtcta ctttccgagc
     3241 acgaactccg acctacggtt cttggcccaa gcctcctcgt tgtgacccgc gtcaaccggc
     3301 ctttcctcgc cgtagctcac tctcgagagc ctttgcccgt ccacgccctg ggagacgaga
     3361 tagtcgtagg cggccttcgc cctccgctcg cccagggcga gattgtactc gttggtgccc
     3421 ctctcgtcgc aatgcccctc gatcgtgacc ttgagaccgg ggttcgtctt gagataggct
     3481 gcaagctcgt tcagggtggc aatggactcc gatgtgagat cgaaccggtc gaagtcgaag
     3541 tagatcttct tgtcgaagac gaaagactcc tggggggccg ggggtgccgc accctccacc
     3601 tggatgcctt gatccccttc cggcgc
//
LOCUS       DAOSCR010000244         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16335_length_3618_cov_3.246422, whole genome shotgun sequence.
ACCESSION   DAOSCR010000244 DAOSCR010000000
VERSION     DAOSCR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16335_length_3618_cov_3.246422"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLT69_06955"
     CDS             <1..333
                     /locus_tag="PLT69_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOJ14211.1"
                     /translation="PRPPVLRRDPELAQGVEVVERERLVAVLEELRLGSSQLADDETR
                     LRVGRITGAREMVFGAYMVVGPTMRLDLRRVDVETGRVVRTASKTAPSGDVQAWVEAA
                     RKAAVDLF"
     gene            complement(423..1121)
                     /gene="tsaB"
                     /locus_tag="PLT69_06960"
     CDS             complement(423..1121)
                     /gene="tsaB"
                     /locus_tag="PLT69_06960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOJ14212.1"
                     /translation="MKVLGLDTSTTHISVAVCIDGDTACEIGFETGSGHAGIILSVVD
                     EVLRRSGTRKEDLEMIAVGLGPGSFTGLRIGIATAKGLAASLGCAVAGVLSLDALAFG
                     ALPSPLPVVTVVDARKGEVFCAVYDTHGTRLMGPANVTPEEAARLAEGPALFVGNACA
                     PYRGIFSGALGDLYVEAPGHLRHPRASVVAALGVKAASSGSGLEIMPVYVRESDAALS
                     LQRRSAKPRGAPRG"
     gene            complement(1126..2199)
                     /gene="rseP"
                     /locus_tag="PLT69_06965"
     CDS             complement(1126..2199)
                     /gene="rseP"
                     /locus_tag="PLT69_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOJ14213.1"
                     /translation="MITILAFVIVLGILIFIHELGHFLAAKASGVGVLRFSLGFGPAV
                     VRKRIGETEYQVAAFPLGGYVKMLGEDPEDKDRVTHIDKRRSFAHKRLWVKAAIVFAG
                     PLFNVLLAVVVYTAIAWTGVPYLLPVIGEVQKGSPADKAGFLPGDLIRSIAGVPVDEW
                     EDVALVLNDTGGSHVVRVEVVREERVFVLNVRPEMQKAKNIFGEEIVRPMIGITASDK
                     TSLKRYGVFGGVSYGVSQCYRIVKLTAVAFSKMVKGTLSVKESLGGPILIAQVSGQTF
                     KAGFLPFVSLIAFISINLAIINLLPIPVLDGGHLLLFLIEGVTGKPVEGRPREVAQQI
                     GLFILVMIMLFAFYNDIVRIFRG"
     gene            complement(2196..3323)
                     /gene="dxr"
                     /locus_tag="PLT69_06970"
     CDS             complement(2196..3323)
                     /gene="dxr"
                     /locus_tag="PLT69_06970"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOJ14214.1"
                     /translation="MKRVAVLGSTGSIGESALDVIRMRPDRFVLVGLASGGNVERILE
                     QALAFGVEIVGLARPKGPIPPGIEVITGENAASEVLRRSRPDIVVNGITGASGFLPSL
                     EAVRMGSVLALANKESLVVGGKFLTEEAAAHGSVIVPVDSEHSAVFQCLSGEPREAVR
                     RIVLTASGGPFRDRPRETFGAITPQEALNHPTWRMGRRITVDSATLMNKGLEMIEACW
                     LFGVSMDMVEVVVHPQSIVHSMVEFSDSSIKAQLGLPDMRTAIAYALSWPDRLDLPLG
                     RLSFRAPLALEFLPPDEEKFPALSVAKKAMERPEVLPCVMNAADEVAVEAFLSGRIRF
                     DEIVALVKKTMDMFGAFKVETPDELVELDRQARKALEAMLA"
     gene            complement(3324..>3618)
                     /locus_tag="PLT69_06975"
     CDS             complement(3324..>3618)
                     /locus_tag="PLT69_06975"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ14215.1"
                     /translation="FLAMILGYASSLVYSLASEAIGVDTLSRAYPWWVLVSMSACIAA
                     LDLAGDLTASLFKREFQIKDLGTLIPGHGGMLDRMDGIIPVGCLLYIASRVVT"
BASE COUNT          629 a         1197 c         1201 g          591 t
ORIGIN      
        1 ccacgaccgc cggtgctgcg ccgtgacccc gagctggcgc agggcgtcga ggtggtggag
       61 cgcgagaggc tcgtggctgt cctcgaggag ctcaggctcg ggtcgtcgca actcgccgac
      121 gacgagacaa ggctccgcgt gggcaggata acgggtgcgc gcgagatggt cttcggcgcg
      181 tacatggtcg tgggccccac catgaggctg gacctcagga gggtggacgt ggagacgggt
      241 cgcgtggtga ggaccgcgtc caagaccgcg ccctcgggcg acgtgcaggc gtgggtcgag
      301 gcggcccgca aggcggccgt cgacctcttt tgaatccttg aaccgggggt gtctggcggg
      361 tctcgaggcc ggccgggata cgcccgaggg gagccgcccg ttgccgggcc tccccttgcg
      421 gctcagcctc ggggggcccc ccttggcttc gccgatcgcc tctggaggga gagcgccgcg
      481 tccgactccc gcacgtagac cggcatgatc tcgaggccgg acccgctgga agcggccttt
      541 acgcccaagg ccgcgaccac cgaggcccgc gggtgccgga ggtgcccggg cgcctcgacg
      601 tagaggtctc cgagcgcccc ggagaagatc ccccggtacg gtgcgcaggc gttgcccacg
      661 aacagggctg gtccttccgc gagccttgca gcctcctcgg gcgtcacgtt cgcaggtccc
      721 atcaagcgcg tgccgtgcgt gtcgtacacg gcacagaaca cctcgccctt cctcgcatcc
      781 accacggtca ccacgggcag agggctcggc agtgcaccaa aggccagcgc gtccaggctc
      841 aggacgccgg cgacggcgca tcccagggat gccgcgagcc ctttcgccgt ggcgatgccg
      901 atgcgcagcc cggtgaagga cccgggcccg agacccaccg cgatcatctc aaggtcttcc
      961 ttgcgtgtgc ccgacctgcg gagaacctcg tccacgacgc tcaggatgat gccggcgtgg
     1021 ccggatccgg tctcgaagcc gatctcgcag gccgtgtcgc cgtcgatgca cacggcgacg
     1081 ctgatgtgcg tcgtcgacgt gtccaggccg aggaccttca tgcgcctagc ccctgaagat
     1141 gcggacgata tcgttgtaga aggcgaacag catgatcatg acgaggatga agagcccgat
     1201 ctgctgggcg acctcgcggg gtctcccctc gacaggcttg ccggtcactc cctcgatgag
     1261 gaagaggagg aggtgcccgc cgtcgaggac cgggatgggc agcaggttga tgatggccag
     1321 gttgatgctg atgaaggcga tgagggacac gaagggcagg aaacccgcct tgaaggtctg
     1381 gcccgagacc tgggctatga ggatggggcc gccgaggctc tccttgacgc tcagggtgcc
     1441 tttcaccatc ttcgagaacg ccacggccgt gagcttcacg atgcggtagc actgcgagac
     1501 cccgtatgag acgcccccga agacgccgta acgcttcagc gaggtcttgt cggacgcggt
     1561 gatcccgatc atcggcctga cgatctcctc gccgaagatg ttcttggcct tctgcatctc
     1621 cggccggacg ttgagcacga agaccctctc ttccctcacc acctcgaccc tcaccacgtg
     1681 agacccgcct gtgtcattga ggacaagggc gacgtcttcc cactcgtcca cgggcacccc
     1741 ggctatcgat cgtatcaggt caccggggag gaacccggcc ttgtccgcag gcgacccctt
     1801 ctgcacctcg cctatcaccg ggaggaggta ggggacgccc gtccatgcga tcgccgtgta
     1861 cacgacaacg gcgaggagca cgttgaacag ggggcctgcg aagacgatgg ccgccttgac
     1921 ccaaagcctc ttgtgggcga acgaacgacg cttgtctatg tgcgtgaccc ggtctttgtc
     1981 ctcagggtcc tcccccagca tcttgacgta gccgccgagc gggaaggcgg ccacctggta
     2041 ctcggtctcg ccgatcctct tcctgacaac ggcgggaccg aagccgaggg agaacctcaa
     2101 gacacccacg ccgctcgcct tggccgccag gaagtggccg agttcgtgga tgaatatgag
     2161 gataccgagg acgatgacga aggcgaggat cgtgatcatg ccagcattgc ctctagtgcc
     2221 tttcttgcct gcctgtcgag ctcgacgagt tcatccgggg tttctacctt gaacgcaccg
     2281 aacatgtcca tggtcttttt cacgagcgcg acgatctcgt cgaagcgtat cctgccggag
     2341 aggaaggcct ccacggccac ctcgtcggcg gcgttcatga cgcagggcag cacctccggc
     2401 ctttccatgg ccttcttcgc caccgacagc gcagggaact tttcctcgtc cggcggcagg
     2461 aactcgaggg ccaaaggcgc cctgaacgag aggcggccga ggggcaggtc caggcggtcc
     2521 ggccaggaga gggcatacgc tatggcagtg cgcatgtcgg gaaggccgag ctgcgccttg
     2581 atgctcgagt ccgagaactc caccatcgag tgcacgatgc tctgggggtg gacgaccacc
     2641 tcgaccatgt ccatgctcac gccgaagagc cagcacgcct cgatcatctc caggcctttg
     2701 ttcatgagcg tcgccgaatc cacggtgatg cgtcttccca tgcgccaggt cgggtggttg
     2761 agcgcctctt ggggggttat ggccccgaag gtttccctgg ggcggtccct gaagggcccg
     2821 cccgaagcgg tgagcacgat gcgtcgaacc gcctcccgcg gctcgccgga gaggcactgg
     2881 aacacggccg aatgctcgga atccaccggg acgatgaccg atccgtgcgc cgccgcctcc
     2941 tcggtgagga atttcccccc caccaccagg gattccttgt tggccagcgc cagcaccgac
     3001 cccatgcgca ccgcctccag ggaaggcaga aagccggaag cacccgttat gccgttgacc
     3061 acgatgtccg gcctcgagcg cctgaggacc tccgaggcgg cgttctcgcc ggtgatcacc
     3121 tcgattcctg gtggtatcgg gcccttcggc ctggcgaggc ccacgatctc cacgccgaag
     3181 gcgagggcct gctccaggat gcgctccacg ttgccgccgg aggccaggcc gacgagcacg
     3241 aacctgtccg gcctcatgcg gatcacgtcg agggcggact cgcctatgga ccccgtggac
     3301 cccaggacgg caacccgctt catctaggtc acgaccctgg atgcgatgta gaggaggcaa
     3361 cccacgggga tgatgccgtc catgcggtcg agcatcccgc cgtgacccgg gatgagcgtg
     3421 ccgaggtcct tgatctggaa ttcccgcttg aacagggagg ccgtcaggtc gccggcgagg
     3481 tcaagtgctg ctatgcacgc ggacatggag accagcaccc accacgggta ggccctcgag
     3541 agcgtgtcca cgcctatcgc ctcgctcgcc agggagtaga ccagggagga tgcgtacccg
     3601 aggatcatgg cgaggaac
//
LOCUS       DAOSCR010000245         3604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16416_length_3604_cov_3.260919, whole genome shotgun sequence.
ACCESSION   DAOSCR010000245 DAOSCR010000000
VERSION     DAOSCR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16416_length_3604_cov_3.260919"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /gene="ald"
                     /locus_tag="PLT69_06980"
     CDS             <1..934
                     /gene="ald"
                     /locus_tag="PLT69_06980"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOJ14216.1"
                     /translation="DSAELIQEADMIVKVKEPVEAEYRFLREGLVLFTYLHLAANEAL
                     TRELMSRRVIAIAYETIQLDDGSLPLLAPMSAIAGRLAVQVGCACLEASHGGKGILLS
                     GVPGVRPGKVAIIGAGVSGVNAAHLAIGLGAQVTVLDINQQRLNYLEDIFHSRAVILL
                     ANAGNVEESVVDADLVICSVLIPGAQAPHLITRDLLRKMEPGSVLVDIAIDQGGCAET
                     SRPTTHDNPTYVEECIVHYCVANMPGAVPRTSTLALTNATLPYVLLLADKTWQGALKE
                     NPALARGLNVVDGAVTNRKVAEAFAMEYVVYRSA"
     gene            955..2589
                     /locus_tag="PLT69_06985"
     CDS             955..2589
                     /locus_tag="PLT69_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ14217.1"
                     /translation="MDRPRDVTLTNAFLWAFRPLQCFRGGMRVREGRIARIFRHGEEG
                     DPGGTVIDLMGMHVIPGLMDAHRHFFVTAFMSMHGDASTWKSKEDALDAIDQACRSNR
                     DPGGWVLFSRMDHTAWRVPDPPSLREIDAVAGKTPVLVADTTLHRGLVSSQALLRSGV
                     DPQALRVKGDLDTLRDGSPRGTVWEDAFGRVASAFYRETFSPLGREEKRNVVLREARR
                     CLAMGLTHVHDPGVPSDVQELLEDVQGYTPLKISWSVTAHESMFAPPGPRDLEHALQS
                     GHAPKSVKFFLDGAHRTAASMPLAGAVRAGLRAARDCLNERSLWPMRLLLEQKIVVKG
                     TRVVMPYLRFPDTAELVEKARPFAEKGYRLVMHALGNVAARQAADVVRRLRPAGASVE
                     HLLIMGEDDLDDFAGCGATASLQPGFIPSYARTIEKKGALPTLKAFPLRSLLDRGVKV
                     CVSSDGPCGVDDPLHNIRRAVDRKKDDGTVFDPGERISAQEALTAGTEGAAHSLGIDW
                     QGLVEGVPATFCVVDGDPFQDTSKVVQTWIDGVQAR"
     gene            2658..>3604
                     /locus_tag="PLT69_06990"
     CDS             2658..>3604
                     /locus_tag="PLT69_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ14218.1"
                     /translation="MKPAKVERMMDGKERDYVQHLFGKHISMGQVRYLRCAHLDVYER
                     DRKGACFVDAVTGKRFFDCFSSAGCFNVGRGNERVKAALEDALARHDMGSHLLLSEHK
                     VRFAERLASLCPGDLDKVVLCASGADACAGAIKLAKGATGRNDVITMNKAYHGHEGFS
                     LSANGKDYYKELFLPLMPGFHVVEFNDLDAVRRVASENTAAVVLEPVQGEGGIHVATK
                     EYMTGLRKLCDELGVMLIFDEVQTGFGRTGTMWAMEHYDVIPDIMFTAKSISGGLYPN
                     GAVVYRAIEPLVSYVEKNPLFHDSHGGGSDLGCIVSQAVL"
BASE COUNT          670 a         1120 c         1201 g          613 t
ORIGIN      
        1 agacagcgca gagcttatcc aggaagcaga catgatcgtc aaggtgaaag aacccgtcga
       61 ggcggagtat cggttcctcc gcgagggcct cgtcctgttc acttacctcc acctggcggc
      121 gaacgaggcg ctcacccggg agctcatgtc aaggagggtc atcgctatcg cctacgagac
      181 gatccagctc gacgacggtt ccctgcccct gctcgcgccc atgagtgcga tcgcgggaag
      241 actcgccgtc caggtcgggt gtgcgtgcct cgaggcatcg cacgggggca aggggatcct
      301 cctgtccggc gtgcccggcg tgaggccggg caaggtggcc atcatcgggg ccggggtctc
      361 tggtgtgaac gccgcccacc ttgccatagg gctcggggcc caggtgaccg tcctcgacat
      421 caaccagcag cggctcaact acctggagga catcttccac tcccgcgccg tgatcctctt
      481 ggccaacgcg ggaaacgtgg aggagagcgt cgtcgatgca gacctcgtga tctgctccgt
      541 gctcataccc ggggctcagg ccccgcacct catcaccagg gacctgttga ggaagatgga
      601 gcctggctcg gtgctcgtgg acatagccat cgaccagggc gggtgcgccg agacgagcag
      661 gcccacgacc catgacaacc ccacctacgt ggaagagtgt atcgttcatt actgcgtggc
      721 caacatgccc ggggcggtcc cccggacatc caccctcgcg ctcaccaacg ccaccctgcc
      781 ctatgtgctc ctgcttgcgg acaaaacctg gcagggcgcc ttgaaagaga accctgcgct
      841 cgcgcgcgga ctcaatgtcg tcgacggcgc cgtcacgaac cggaaggtgg ccgaggcgtt
      901 cgccatggaa tacgtggtat accgctcggc gtgacacgaa cgaggaggga aaggatggac
      961 cgcccacggg atgtcacgct caccaacgcc ttcctctggg ccttccgccc gctccagtgc
     1021 ttcagagggg ggatgcgcgt gagagaaggt cggatagcgc ggatctttcg ccacggtgag
     1081 gagggtgacc cgggcggcac ggtcatagac cttatgggca tgcacgtcat accggggctc
     1141 atggacgcgc accggcattt cttcgtgacc gccttcatga gcatgcacgg tgatgcctcg
     1201 acgtggaagt cgaaggagga tgccctcgac gccatcgacc aggcgtgcag gtccaaccgg
     1261 gatcccggtg gatgggtgct cttttcccgc atggaccata ccgcatggag ggttcccgat
     1321 cccccgtccc tgcgggaaat agacgcggtc gccggaaaaa cgccggtctt ggtcgcagac
     1381 accacccttc atcgaggcct tgtctcgtct caggccttgc tcaggtcagg ggtcgacccg
     1441 caggcgctgc gtgtcaaggg cgacctcgat accctccgag acggctcacc ccgtggtacc
     1501 gtgtgggagg atgcattcgg cagggtcgcg tccgccttct accgggaaac gttcagcccg
     1561 ctcggcaggg aggaaaaacg caacgtcgtt cttcgtgagg cccgcaggtg cctcgccatg
     1621 ggcctcacgc acgtgcacga tccaggcgtt ccctcggatg tccaggagct gctcgaggac
     1681 gtccaagggt ataccccgct caagatcagc tggtctgtca cggcgcacga gagcatgttc
     1741 gctcctcccg ggccccgaga cctcgagcat gcccttcaga gcgggcatgc acccaagagc
     1801 gtgaagttct tcctggacgg tgcacaccgg acggcggcct ccatgccgct tgcaggtgcg
     1861 gtcagggcgg gcctcagggc ggctcgcgac tgcctgaacg agcggagcct gtggcccatg
     1921 cggctcctcc tcgagcagaa gatcgtcgtc aagggaacaa gggtggtcat gccgtacctg
     1981 aggtttccgg ataccgccga gctcgtcgag aaggcgcgac cgtttgcgga gaagggatac
     2041 cggctcgtca tgcacgccct gggaaacgtg gcggccaggc aggcggccga tgtcgtcagg
     2101 cgcctgaggc ctgcaggggc ctcggtggag cacctcctca tcatgggaga agacgacctg
     2161 gacgacttcg ccggctgcgg cgcgacggcg tccctccagc ccgggttcat cccgtcctat
     2221 gcccgcacca tcgagaagaa gggcgcgctt cccaccctca aggcgtttcc gctcaggagc
     2281 ttgctcgaca ggggggtgaa ggtgtgcgtg agctcggacg ggccctgcgg ggtcgatgac
     2341 ccgctccaca acatccggcg tgccgtggac aggaaaaagg acgacggcac ggtgttcgat
     2401 cccggtgaac gcatctctgc gcaggaggcg ctcacggccg ggacagaggg ggctgcgcat
     2461 tctctgggta tagattggca gggtcttgtc gagggtgtgc ccgcaacgtt ctgcgtcgtc
     2521 gacggggacc cgtttcagga taccagcaag gtcgtgcaga cgtggataga cggggtgcag
     2581 gcccgatgat cgggctacag ggcgtttcgg ctcaagaacg tggcatgagt tttctcatca
     2641 agcacagggg ggattccatg aaaccggcaa aggttgaaag gatgatggat ggaaaggaga
     2701 gggactatgt ccagcacctc ttcggcaaac acatctcgat gggccaggta cgctacctca
     2761 ggtgcgccca cctcgacgtg tacgagcgcg acaggaaggg cgcgtgcttc gtcgatgccg
     2821 tgacgggaaa gcgcttcttc gactgtttca gctcggcggg atgtttcaac gtgggccgcg
     2881 gcaacgaacg ggtcaaggct gcgctcgagg atgcgctcgc gaggcacgac atgggatcgc
     2941 accttctcct gtcggagcac aaggtgcgct tcgccgaacg gctggcctcc ctgtgcccgg
     3001 gagacctcga caaggtggtg ctctgcgcga gcggggcgga tgcctgcgct ggcgccatca
     3061 agctcgccaa gggcgccacg ggcagaaacg acgtgatcac catgaacaag gcgtaccacg
     3121 ggcacgaggg gttcagcctg tcggccaacg ggaaggacta ctacaaggag ctgtttctgc
     3181 ccctcatgcc cggtttccac gtggtcgagt tcaacgacct tgatgcggtg agacgggtcg
     3241 cgtccgagaa cacggcggcc gtggtcctcg aaccggtcca gggcgagggc gggatccacg
     3301 ttgcgaccaa ggagtacatg acgggcctca ggaagctctg cgacgagctc ggcgtcatgc
     3361 tcatcttcga cgaggtgcag accggcttcg ggcgtaccgg taccatgtgg gccatggaac
     3421 actacgacgt gatacccgac atcatgttca cggcgaagtc gataagcggg gggctctacc
     3481 cgaacggcgc cgtggtgtac agggccatag agccgctcgt gagctatgtg gagaagaacc
     3541 cgctcttcca cgactcccac ggcggcggct cggacctcgg ctgcatcgtg tcccaggcgg
     3601 tact
//
LOCUS       DAOSCR010000246         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16496_length_3591_cov_3.251414, whole genome shotgun sequence.
ACCESSION   DAOSCR010000246 DAOSCR010000000
VERSION     DAOSCR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16496_length_3591_cov_3.251414"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="PLT69_06995"
     CDS             complement(<1..2038)
                     /locus_tag="PLT69_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HOJ14219.1"
                     /translation="MSRLAYRKVKIISAVVAVSLWCLGSTANAVAFAPLDAAEARNRC
                     AAVDQEAYPDADLVVVARQTHVEYQVDGTSEEWFEQYVKVLTERGRRDACTVSSSFTL
                     PYNTTVFQLVEVISSDGSVRKVDIEKNVKETIDASQMASNIYNPNDRLLQVTIPGLVV
                     GDTVHFITKDDFVKARVPGTFSDYVTFEETNPILYAQYTVVAPPSLPLAAMALRDEVE
                     GTVTFVKTTEAGKIVYRWVAKNVPMAFAEPEMPPMYTQVQRLLVSTIADWREISRWYW
                     RLSMPHIRATTPAMAAEVKRLTKGKATRDERIRAVFFWVSRNIRYLGITAESEAPGYE
                     PHPASMTYDRRTGVCRDKAALLVAMLRLAGFEAYPVLIMNGPKKDPEVPQPFFNHAIA
                     CVRNKDGSYLLMDPTDENTRELFPSYLGNQSYLVATPSGETLMTSPVESALKNTMRIS
                     TEGAVDAMGRLSAKTSLTFGGVNDNAYRGHLMRLSSRERRLFFEKTLLRAVPGAVLEA
                     FELRPGDLADVSRPLEAHLSFKAEGFLVRGTGKAALPLLRFGGAVGVLPYVTRGMGLR
                     ERRFPYVTETTCAVDESLSLRLDPSLGVAEVPGGVESRRSDGCRWKREMRVQGQRLEM
                     RDTLSMDLTEYSPDAYRALQEVLAAQERSDRLLPTFKTARAIDAADAWYTGFASD"
     gene            2266..2460
                     /locus_tag="PLT69_07000"
     CDS             2266..2460
                     /locus_tag="PLT69_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14220.1"
                     /translation="MTLDRSLCAICAWRGDCNKKFMQGKGVHCPDFTRDLTIRAKEEE
                     KQEKTASNSGQKKGSGIRFL"
     gene            complement(3007..>3591)
                     /locus_tag="PLT69_07005"
     CDS             complement(3007..>3591)
                     /locus_tag="PLT69_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906719.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOJ14221.1"
                     /translation="VFEAADGILIPGGFGERGTEGKILAAEFARTRKVPFFGICLGMQ
                     LAVIEFARNVCGLSGANSTEFDQSTAYPVIDFLPGQKGLTRKGGTMRLGAYACRLEPG
                     TLAMRAYGTDVISERHRHRYEVNPAFEESLVDKGIVVSGRNPQSALVEIVEVPGHPWF
                     LGCQFHPEFKSKPMSPHPLFKDFIRASLEYRRNR"
BASE COUNT          611 a         1208 c         1091 g          681 t
ORIGIN      
        1 cgtcgctcgc gaagcccgtg taccatgcat cggctgcgtc gatggcccga gccgtcttga
       61 aggtgggcag gagacggtcg gagcgctcct gggccgcgag cacctcctgg agagcgcggt
      121 aggcatcggg ggaatactcg gtgaggtcca tgctgagcgt gtccctcatc tcgagcctct
      181 gcccctgcac cctcatctcc cgtttccacc tgcacccgtc ggacctcctg gactccaccc
      241 ccccggggac ctccgcaacc cccaacgacg ggtccagtcg aaggctgagg ctctcgtcaa
      301 cggcgcatgt ggtctcggtc acgtacggga acctgcgctc tctcaacccc atgccccgcg
      361 tcacatacgg caggacgccc acggcgcccc cgaagcgtaa aagaggcagt gcggccttcc
      421 ccgtccccct gacgaggaac ccttcggcct tgaaggaaag gtgtgcttcg aggggcctcg
      481 agacgtcggc gagatcaccg ggcctgagct cgaaggcctc gaggacggcg ccgggtaccg
      541 ccctcaggag cgtcttctcg aagaagagcc tcctctcccg ggaggagagc ctcatgaggt
      601 gcccccggta ggcgttgtcg ttgacgccgc cgaacgtgag cgacgtcttg gccgacagcc
      661 tccccatggc gtccaccgcg ccctcggtcg atatgcgcat cgtgttcttg agcgcgctct
      721 ccaccgggct cgtcatgagc gtctcgcccg agggcgttgc caccaggtag ctctggttgc
      781 cgaggtaaga ggggaagagc tccctcgtgt tctcgtccgt cgggtccatg aggagatagg
      841 agccgtcctt gttcctgacg caggcgatcg cgtggttgaa gaacggctgg gggacctccg
      901 ggtccttctt cgggccgttc atgatgagca cggggtaggc ctcgaagccg gcgagcctca
      961 gcatcgccac caggagggcg gccttgtccc ggcacacgcc cgtgcggcgg tcgtaggtca
     1021 tggatgcagg gtggggctcg tacccaggcg cctcgctctc cgcggtgatc ccgaggtacc
     1081 tgatgttcct ggagacccag aagaagacgg ccctgatcct ttcgtccctc gtggccttgc
     1141 ccttcgtgag cctcttcacc tcggcggcca tggccggggt cgtggcccgt atgtgtggca
     1201 tggacaggcg ccaataccac ctcgatatct ccctccagtc ggctatggtg ctcacgagca
     1261 ggcgttggac ctgggtgtac atgggcggca tctcgggctc ggcaaaggcc atggggacgt
     1321 tctttgccac ccacctgtac acgatcttgc ccgcctcggt cgtcttcacg aaggtgacgg
     1381 tcccctccac ctcgtccctg agcgccatgg ccgcaagcgg gagcgaaggg ggcgccacga
     1441 ccgtgtactg cgcgtagagg atggggttgg tctcctcgaa cgtgacgtag tcgctgaagg
     1501 tccccggcac ccgggccttg acgaaatcgt ccttggttat gaagtggacc gtgtccccga
     1561 cgacgagccc ggggatggtc acctggagga gcctgtcgtt ggggttgtag atgttggacg
     1621 ccatctggga cgcgtcgatc gtctccttca cgttcttctc gatgtcgacc ttcctgacgg
     1681 aaccgtcgct ggagatcacc tcgacgagct ggaagacggt cgtgttgtac gggagcgtga
     1741 acgacgacga gaccgtgcag gcatctcgcc tccccctctc ggtgaggacc ttgacgtact
     1801 gctcgaacca ttcctcggac gtgccgtcca cctggtactc cacgtgggtc tgcctcgcca
     1861 cgaccaccag gtccgcatcg ggatacgcct cttggtccac ggcggcgcac ctgttcctcg
     1921 cctccgcggc gtccaagggc gcgaaggcca cggcgttagc cgtcgacccg aggcaccaca
     1981 agctcaccgc cacaactgcg gaaattatct ttacttttct gtatgccagc ctcgacatgg
     2041 tatccctcac cctctcatga cttcgctgtc cagcggccta tgaagcggat cctgcaagcg
     2101 ggaccttcct gcacacgatg catggatcaa tcccacactc ggcaatcggg gcttttcatt
     2161 cctataaaaa cgagccggtt tcgccgctgt tcttttcaca aaacgtccgt tgtctcgatg
     2221 atttgtattg acaaggccgc atagggtctt agtataattc acaccatgac actggacaga
     2281 tcgctgtgcg caatctgtgc ctggagaggt gactgcaaca agaagttcat gcagggcaag
     2341 ggagttcatt gcccggactt cacgagagac ctcaccatcc gggcgaagga agaggagaag
     2401 caggagaaaa cggcgtcgaa cagcggccag aagaagggct cggggattcg cttcctgtaa
     2461 tcgaagcggc cgttcctccc cactttcaac gcgctcctct ccctcagatg caggcgggaa
     2521 ggactcggtt cccgagggtg aactgctgcc gagtgtcatc catctcacat ccaacccctc
     2581 cccccgtggt gagaaaacct atcaggcatg caccgtcggc acagccgcgg cctcgtgcag
     2641 tgggcacatt tccgttccag ggggcacgcc gaacgctctt gactcgtccc acggcaccgt
     2701 cgcttcctcg aaggggcccg caagccttcg gccgaaggtt tccccttcgc ctgcctcatg
     2761 gtggaaaacg gtgcaggtct gccccgaccg aacccgccgc aaccaccctt gcgtgagatc
     2821 acggcgccct gctcgaaacg aagacccccc acatcggcca gggtatgggc gtatgcaacc
     2881 catccccccg gtcgcttgcc ctgtcggagc catcggtgta cacggccgaa caggacaccg
     2941 gggccggcct tgccttcccg aacacccggt ccaggctgac ggttcaccgg ccggtcgccc
     3001 tctctctcag cggttccgcc tgtactccag gctcgccctg atgaaatcct tgaacagcgg
     3061 gtgcgggctc atgggcttgg acttgaactc ggggtggaac tggcaaccga ggaaccaggg
     3121 gtggcccggc acctcgacga tctccacgag ggcgctctgg gggttcctgc cgctcacgac
     3181 gatgcccttg tcaacgaggc tctcctcgaa cgcggggttg acctcgtagc ggtgcctgtg
     3241 cctctcgctg atgacgtcgg tgccgtaggc cctcatggcc agcgtcccgg gctcgagcct
     3301 gcacgcgtac gccccgagcc gcatggtccc acccttgcgc gtgagcccct tctggcccgg
     3361 gaggaaatcg atgaccgggt acgccgtgga ctggtcgaac tcggtgctgt tcgcgccgct
     3421 caacccgcag acgttccttg cgaactcgat cacggcgagc tgcatcccga ggcagatgcc
     3481 gaagaagggc accttcctcg tccgtgcgaa ctccgccgcc aggatcttgc cctcggtacc
     3541 cctctcgccg aacccgcccg ggatgagtat gccgtctgct gcctcgaaca c
//
LOCUS       DAOSCR010000247         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16549_length_3584_cov_3.096061, whole genome shotgun sequence.
ACCESSION   DAOSCR010000247 DAOSCR010000000
VERSION     DAOSCR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16549_length_3584_cov_3.096061"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..732
                     /locus_tag="PLT69_07010"
     CDS             253..732
                     /locus_tag="PLT69_07010"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ14222.1"
                     /translation="MELDATDKAILSLAEGDLEISVRPYGIMAQRLGVGEEELIERLR
                     RLRRRGIVREVKAVLRHTRAGVGANAMVVWAVPSGEVDTAGKVMASFESVTHCYEREG
                     FEPYTVFSMIHARTRDDLVKTVHDISRRTGVKEYKVFWSVRELKKTSMRYFKDRGDR"
     gene            729..2009
                     /gene="hemL"
                     /locus_tag="PLT69_07015"
     CDS             729..2009
                     /gene="hemL"
                     /locus_tag="PLT69_07015"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOJ14223.1"
                     /translation="MTSETLFEEAKRVIPGGVNSPVRAFGRVGGTPRFIERGRGAYLW
                     DADGRKYVDFVLSWGPLILGHCHPDVLEALMHQASLGTSYGAPTRLEVEMARMVCEAF
                     PSMEKVRFVNSGTEALMSAVRLARAATGRDLVVKFDGCYHGHSDCLLVKAGSGVLAQG
                     VPDCPGVPGAVVSSTVVLSYNDTQAFEEFMGEHGREVAAVVVEPVAGNMGVVPPERGF
                     LESARDQTRRHGALLVFDEVITGFRFCYGGYQHISGITPDITCLGKIVGGGLPAAAFG
                     AKAEIMNLLAPVGPVYQAGTLSGNPLAMAAGIATLRVLREQDPYRELARNMAVFANGI
                     GQAAREAGIPVTVNVMGSMATVFFTRGPVRGLADVEAADTALYARFFHAMLDRGVYLA
                     PSPYEALFVSTAHTPDIIDRALAAAHEAISSLSP"
     gene            complement(2061..>3584)
                     /gene="lon"
                     /locus_tag="PLT69_07020"
     CDS             complement(2061..>3584)
                     /gene="lon"
                     /locus_tag="PLT69_07020"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ14224.1"
                     /translation="PTSSEYVTIRTYLDWILDLPWKESTEDNLDIEHAERILEEDHFD
                     LKKPKKRILEFLSVKKLKKDLKGPILCFVGPPGVGKTSLGKSIARAIGRKFVRISLGG
                     VRDEAEIRGHRRTYIGAMPGRIIAGIKTAGTVNPVFMLDEIDKLTRDIHGDPASALLE
                     ALDPEQNNHFTDHYLNVPYDLSRVFFIATANVVETIPPALKDRMEIVEIEGYTHEDKL
                     QIARNYLIPRELEANGLLGCSIDFTDEAVSHIIRHYTRESGVRNLQREIAAVLRGIAS
                     RVAKGCTEGFVVDERFIEDALGPVRFMPELTQRTGMPGIATGLAWTPFGGEILFVESV
                     LVEGKEEMILTGQMGDVMKESARIALSIVKSMGLKPEKDQGIHIHVPAGAIPKDGPSA
                     GVTIVTSLMSLLTGTPVREKLAMTGEITLRGVVMPVGGIKEKVLAARRAGITDIVMPM
                     MNEKDLSDIEPYVKRDLTIHLVENIEEVLAVAFPARHHGLLAELHEPVEIPGPRPGC"
BASE COUNT          576 a         1107 c         1248 g          653 t
ORIGIN      
        1 tgaagcggcc cggtgtgttt cgagagcagc ccgagggttt ccatgacgcg gccctggggg
       61 tgagccttga tgggcgggtg cgcgggagct tgcccgcggg gtgcggcgtg cgcacgatcg
      121 tcaccgtggg caggacctct ccggggggcc gaaaggaggc ccgcctgccc gaggttcccg
      181 gaagacccgc ggaccgagtg cgcccacctt gccggttgat tcgcggggcg tgctgtggtg
      241 agatgcatgg tcatggaact cgatgcgacc gacaaggcga tcctctccct tgccgaaggg
      301 gacctcgaga tctcggtgcg cccttacggg atcatggcgc aaaggctcgg cgtcggcgag
      361 gaggaactca tcgaaaggct ccggcgactc aggcgaagag gaatcgtcag ggaagtgaag
      421 gcggtcttga ggcacacgcg ggcgggagtc ggggccaacg ccatggtggt ctgggccgtc
      481 ccctccggcg aggtggatac cgcgggaaag gtcatggcct cgttcgaaag cgtgacccac
      541 tgctacgaac gcgaggggtt cgagccctac acggtgtttt ccatgatcca cgcgaggacg
      601 agggatgatc tcgtgaaaac ggtgcatgac atctcgagga ggaccggggt gaaggagtac
      661 aaggtcttct ggagcgtccg ggaactcaag aagacctcga tgagatactt caaggacagg
      721 ggggatcggt gacgagcgag acgctgttcg aggaggcgaa acgggtcatc cccggcggtg
      781 tgaactcgcc ggtcagggcg ttcgggaggg tcggcgggac gcccaggttc atcgagcgcg
      841 gcaggggggc gtacctctgg gacgccgacg gcaggaagta cgtcgacttc gtgctttcct
      901 gggggcctct catcctggga cactgccacc cggacgtgct cgaggccctc atgcaccagg
      961 catcgctggg cacgagctac ggcgcgccga ccagactgga ggtcgagatg gcgcgcatgg
     1021 tctgcgaggc cttcccgtcc atggaaaagg tgcgcttcgt gaactccggt acagaggccc
     1081 tcatgagcgc ggtcaggctc gcccgggcgg ccacggggag ggatctcgtg gtcaagttcg
     1141 acgggtgtta ccacgggcac tcggactgcc tcctggtgaa ggcgggctca ggggtgctcg
     1201 cccagggggt ccccgactgt cccggggtgc cgggcgcggt cgtgtcgtcg accgtcgtgc
     1261 tctcctacaa cgatacgcag gccttcgagg agttcatggg ggagcacggc cgcgaggtcg
     1321 ctgcggtcgt cgtggagccg gtggccggaa acatgggcgt ggtcccgccc gagagggggt
     1381 tcctcgagtc cgcgagagac cagaccaggc gccacggcgc gctgctggtc ttcgacgagg
     1441 tgatcacggg tttcaggttc tgttacggtg gttaccagca catctcaggg atcacgccgg
     1501 acatcacctg cctgggtaag atcgtcgggg ggggtctgcc tgccgcggcg ttcggcgcca
     1561 aggccgagat catgaacctg ctcgcgcccg tgggccctgt ctaccaggcc ggtaccctct
     1621 cgggcaaccc gctcgccatg gcggccggca tcgcgacgct cagggtgctc agggaacagg
     1681 atccctaccg ggagctcgcg aggaacatgg ccgtgttcgc gaacgggatc gggcaggcgg
     1741 cgagagaggc cggcataccc gtgacggtca acgtcatggg ctccatggcc acggtcttct
     1801 tcactcgagg gccggtcagg ggcctcgccg acgtggaggc cgccgacacc gcgctgtacg
     1861 ccaggttctt ccacgccatg ctggatcgag gggtctacct cgcgccgtcc ccatacgagg
     1921 cgctcttcgt gagcacggcg cacacccccg acatcatcga cagggctctg gcagcagccc
     1981 acgaggcgat ctcgagcctc tccccttaag gcccctgaga gagggcccct caccccggca
     2041 ggggaaggcg gaagggtcgg ctagcacccg ggcctgggtc cgggaatctc cacgggctcg
     2101 tggagctcgg ccaagaggcc atggtggcgt gccgggaagg cgaccgcaag gacttcctcg
     2161 atgttctcga cgaggtggat ggtgaggtct ctcttcacgt agggctcgat gtcgctcaaa
     2221 tccttctcgt tcatcatggg catcacgatg tccgtgatac ccgccctgcg cgccgccagg
     2281 accttctcct tgatgccgcc cacgggcatc accacccccc tgagggtgat ctcgcccgtc
     2341 atggcgagct tctctctcac cggtgtgccg gtgaggaggg acatgaggga cgtgacgatg
     2401 gtgacgcccg cagacggccc gtccttcggt atggcgcctg caggcacgtg gatgtggatg
     2461 ccctggtcct tctcaggctt caggcccatg gacttgacga tgctgagggc tatcctcgcg
     2521 gactccttca tgacgtcgcc catctgcccg gtgaggatca tctcttcctt tccctccacg
     2581 agcacggact ccacgaacag gatctcgccc ccgaacggcg tccaggcgag cccggtcgcg
     2641 atccccggca tgcccgtccg ctgcgtgagc tcgggcatga agcggaccgg gccgagggcg
     2701 tcttcgatga accgttcgtc caccacgaag ccctcggtgc aacccttggc caccctggag
     2761 gcgatgcccc tgaggacggc ggcgatctcg cgctggagat tccgaacccc cgactccctg
     2821 gtgtagtgcc tgatgatgtg gctgacggcc tcgtccgtga agtctatcga gcacccgaga
     2881 aggccgttgg cctccagttc cctggggatg aggtagtttc tcgctatctg gagcttgtcc
     2941 tcgtgcgtgt agccctctat ctcgacgatc tccatccggt ccttgagcgc cggcgggatg
     3001 gtctccacca cgttcgccgt ggcgatgaag aagacccgcg acaggtcgta gggcacgttg
     3061 aggtagtggt ccgtgaagtg gttgttctgc tcggggtcca gggcctccag gagcgcagag
     3121 gcggggtcgc cgtggatgtc cctggtgagc ttgtcgatct cgtcgagcat gaagacgggg
     3181 ttcacggtgc cggcggtctt gatgcccgct atgatgcgac cgggcatggc gccgatatag
     3241 gtcctcctgt ggcccctgat ctccgcctcg tccctcaccc cgccgaggga gatgcggacg
     3301 aacttcctgc cgatcgccct ggctatcgac ttgccgaggg aggtcttgcc cacccccggg
     3361 gggccgacga agcacaggat cggtcctttg aggtctttct tgagcttctt gaccgacagg
     3421 aactcgagga tccgcttctt cggtttcttg aggtcgaagt ggtcctcctc gaggatccgc
     3481 tcggcatgct cgatgtccag gttgtcctcc gtgctctcct tccagggcag gtccaggatc
     3541 cagtcgaggt aggtccggat ggtgacgtat tccgaggacg tcgg
//
LOCUS       DAOSCR010000248         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16595_length_3577_cov_2.728847, whole genome shotgun sequence.
ACCESSION   DAOSCR010000248 DAOSCR010000000
VERSION     DAOSCR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16595_length_3577_cov_2.728847"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLT69_07025"
     CDS             <1..718
                     /locus_tag="PLT69_07025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOJ14225.1"
                     /translation="GHTEGIAFALFPKKAGRVFETNDDEDWVRMGTLLGRIHMVGSRR
                     RAPSRVTMHPSSCTRTDLDFLLQGGFVGPEHVHAFEDVCLSVIEEISGLFEGVEFIRI
                     HGDFHRGNLLDRPSEGLMVIDFDDMMEGPAVHDLWLLLPDHAGRARREIELILSGYER
                     FYPFDPSSLRLIEALRFMRFIYYLAWCARQSADYDFRTNHPDWGTDGFWQRELLDLHH
                     QRQVIMEHKVAWEEGRFFEA"
     gene            1195..2307
                     /locus_tag="PLT69_07030"
     CDS             1195..2307
                     /locus_tag="PLT69_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ14226.1"
                     /translation="MATDRTTLNRIVTAFLVAAVTALFVAMIRPFLLTIVLAGVFAAM
                     LQPVYKRVYRLVGGRPSLASLVTLSFLLFACILPLMGLLGVLAGQAISMGRSAKEFLE
                     GLGASGWSAADILGRLGLAGVYEAYRDTIVARMGEIAAKLGQIVMDTVSGVTVITIQS
                     AFFFFVFLYTLFFFIKDGRKILDRFMSYVPLPEGVEQRLVERFTQVATSALKGVFVIG
                     LVQGSLAGLAFRLVGIDKALFWGCVMAILSIIPAVGSAIVWVSASIILLLSGAYLKAA
                     FLIAFCSIVVGGVDNLLRPWLVGRETKMHELMIFFGTLGGIGMFGITGFIIGPVVAAL
                     FITAWDIYSENFSDFLPDGPGAAPGEQKDEQAGTPR"
     gene            complement(2892..3341)
                     /locus_tag="PLT69_07035"
     CDS             complement(2892..3341)
                     /locus_tag="PLT69_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14227.1"
                     /translation="MTTITVIAALTLAMLPGQAGAETRVYVNLGLPALCVDVCGGGYA
                     CDGCVWVAGYWRGCGPSCVWVPGHFEPVVTHRVISRPRVNIHEHNTYIYHYGPRHGRY
                     HDDGWKKDGFKRPPAPGYPRSQGYDRGGQRPYGQGVWKSTAASNRMW"
BASE COUNT          575 a         1202 c         1170 g          630 t
ORIGIN      
        1 ggggcacacc gaaggcatcg ccttcgcgct gttcccgaag aaggcgggcc gcgtcttcga
       61 gacgaacgac gacgaggact gggtccgcat ggggaccctc ctcggccgca tccacatggt
      121 ggggtcccgt cgaagggcgc catccagggt gaccatgcac ccgtcatcct gcacgaggac
      181 ggaccttgac ttcctgctcc agggcgggtt cgtcgggccc gagcacgtcc atgccttcga
      241 ggacgtgtgc ctgagcgtca tcgaggagat atcgggcctg ttcgagggcg tggagttcat
      301 ccgcattcac ggcgatttcc acaggggcaa cctgctcgac aggccgtcag aggggctcat
      361 ggtgatcgac ttcgacgaca tgatggaggg cccggcggtg cacgacctct ggctgctcct
      421 tcccgaccac gcaggaaggg cccgccgcga gatcgagctc atcttgagcg gttacgagcg
      481 cttttacccc ttcgacccgt cgtccctcag gctcatcgag gcgctcaggt tcatgcggtt
      541 catctactac ctggcctggt gcgcgaggca gtcagccgac tacgacttca ggaccaacca
      601 ccccgactgg ggcacggacg gtttctggca gagggaactg ctcgacctgc accaccagcg
      661 ccaggtgatc atggagcaca aggtggcgtg ggaggagggc aggttcttcg aggcctgaac
      721 acccgggcgg acgccccacc ctttttccac cctcctttgc tgcagggcac gggcataaag
      781 gccctgcaca ggtcccgagg caggataccc accggaaaga ggctggacgg cgcggccaca
      841 cgcacaggct gcgcaaggcc cttgccagtc cgggacacgg gtccataccg gcaacgcgcg
      901 acagacctct gcttcaggtc ccttgcccgc atggaacacg gcttttgccg gcttctcggc
      961 cgaggcgcgg tatcgcacgc cccatgcccg cacgggacac ggatcggccg tggcatgtcg
     1021 ccgccccctt tgaatccggc aacgcctcgg ggagaggtct cggccccgca agcccttccg
     1081 ggaccgaagg cgtctcacgc aaccagggcg ccgaccatgc gccggggtta agatgggggg
     1141 aagctgcggc gctggatatc ggccgcctgt catggtaacg tagctgcacg acacatggct
     1201 acggacagga ccaccctgaa caggatcgtg accgccttcc tcgtggctgc cgtcaccgcg
     1261 ctcttcgtgg ccatgatccg cccctttctc ctgacgatcg tgctcgcggg cgtcttcgcg
     1321 gccatgctcc agcccgtcta caagagggtt tacaggctcg tgggcggcag gccgtccctg
     1381 gcttccctcg tcacgctctc ctttctcctc ttcgcgtgca tcctgccgct catgggtctc
     1441 ctgggggtgc tcgcaggcca ggcgatctcc atgggcaggt cggcgaagga gttcctcgaa
     1501 ggcctgggcg cttccggctg gtccgcagcg gacatcctcg gccgcctcgg cctcgcgggc
     1561 gtgtacgagg cctacaggga cacgatcgtg gcgcgcatgg gcgagatcgc tgccaagctg
     1621 ggccagatcg tgatggacac ggtctcgggc gtcaccgtga tcaccatcca gagcgccttt
     1681 ttcttcttcg tcttcctcta caccctgttc ttcttcatca aggacgggag aaagatcctc
     1741 gacaggttca tgtcgtacgt gccgctccca gagggcgtgg aacagaggct ggtggagagg
     1801 ttcacccagg tggccacctc ggccctcaag ggggtgttcg tcatcgggct cgtgcagggc
     1861 tcgctcgcag gcctcgcctt ccggctcgtg gggatcgaca aggcgttgtt ctggggctgt
     1921 gtcatggcga tcctctccat catccccgcc gtgggttctg cgatcgtgtg ggtgagcgcc
     1981 tcgatcatcc tcctgctctc gggcgcctac ctcaaggccg cattcctcat cgccttctgc
     2041 agcatcgtcg tgggcggcgt ggacaacctc ctgcggccgt ggctcgtcgg cagggagacc
     2101 aagatgcacg agctcatgat cttcttcggc acgctgggcg gcataggcat gttcgggatc
     2161 accggcttca tcatcgggcc cgtcgtggcg gcgttgttca tcaccgcgtg ggacatctac
     2221 tcggagaact tctcagactt cctgcctgac gggccggggg cggcccccgg tgagcagaaa
     2281 gacgaacagg ccggcacacc caggtgaacg aagacggaag ctccctggac tttccctgta
     2341 cttgggctgg accttcgggc gtggccgcgc gccgtaccca ccgggcggca tgagggggcc
     2401 ggccaagggg tgccgaggca cccggccctc ccggctggca agcaacgggc atgacgggcg
     2461 gagaggtacg ctcgagaatc agggggcgcc gacgcggccc gggcattgga cgccgtcatc
     2521 gttcacaccc tcggggaggc aagggatacc ttgcctgcgc gcgccgacag ggtgcgagag
     2581 ccttctcagc caggcatcga gaggcaaggg ccagctttga agtcctgcct tgacgggcgg
     2641 gggtcgccac aaaggcccgg cccttccgcg tgcatggccg gcgcggcccg ggtgaagggg
     2701 agcgcatcac cgcaccgccg acgcgaccac cctgtgcctg gagagggagc ccaggcggag
     2761 cgagggaggc ttcctccccg tcgatcccac gtgcgccttc gcttcgctca cgtctggtac
     2821 cggtatgcag gctccaggca ggctcacgga gccgggcacg aaccctttcg tgcccggccc
     2881 cgtctcggcc ttcaccacat gcggttggaa gcggccgtcg acttccagac gccctgccca
     2941 taaggccgct gcccaccgcg gtcatagccc tggctgcgcg gatatccggg cgcagggggt
     3001 ctcttgaagc cgtctttctt ccacccatca tcgtggtacc gcccgtgcct cgggccgtag
     3061 tggtaaatgt aggtgttgtg ctcgtggatg ttcaccctcg gcctggagat cacccggtgc
     3121 gtgaccaccg gctcgaagtg cccgggcacc cagacgcacg aagggccgca tcccctccag
     3181 tagcccgcca cccacacgca gccgtcgcag gcataccccc cgccgcacac gtccacgcac
     3241 agtgcaggca ggccgaggtt cacgtacacc ctggtctcgg ccccggcctg gcccggcagc
     3301 atcgcgagcg tgagtgcggc gataacggtg attgtcgtca ttcttgcggt catgtcatcc
     3361 tcccctgtgt ttgcttggtc accggttatg acgggggtgg gggatgaaat gtctacacga
     3421 cccttgcacg aggagggcga tgggacgata atgcccctcg atatcccaac ggatgcggac
     3481 ggcaccggga acgagcctcg ctccagcgcc gggcgatgca caggagacgg ggctccaggc
     3541 gttgtcgtcc tacccgcccg gtgggaccga ggaacgc
//
LOCUS       DAOSCR010000249         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16599_length_3576_cov_3.341096, whole genome shotgun sequence.
ACCESSION   DAOSCR010000249 DAOSCR010000000
VERSION     DAOSCR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16599_length_3576_cov_3.341096"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLT69_07040"
     CDS             complement(<1..420)
                     /locus_tag="PLT69_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOJ14228.1"
                     /translation="MSEKGQGRHLAYGIASLCLYAACVVAYAVWANHERVTQFQGEID
                     RRLLMAARGLKYMLAPDFHDRAVDAGSIPFDEELRNRRAVNGFCRDADFKWIYTLVEK
                     DGRFFFAAPTVSEEEARQRRSWYFYPYEDIPREFVEAY"
     gene            complement(973..1893)
                     /locus_tag="PLT69_07045"
     CDS             complement(973..1893)
                     /locus_tag="PLT69_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOJ14229.1"
                     /translation="MDMNPREAKGTIFNIQHFSIHDGPGIRTTVFLKGCPLRCAWCSN
                     PESISSAVQIRVTPHRCEGCGRCVAACPRGALSLVGGRVRFDRRGCDDCLVCVDACVN
                     GCISTVGTVIDAQSLVERLVKDRPFYEHTGGGVTVSGGEPLSQPAFTRCVLEGLHRLG
                     IETALDTSGCSPWEAFEEVLPHVDLVLFDIKHPDPARHVEATHADNALILENLRRLAG
                     QVAIWTRTPLVPGYNDGPEVTDEIVSMAREVGAKRCWFLPLHRWGEHKYECLGMPNPY
                     PAFEDVSPEMLEALRRRYLHMDGFVLIEKS"
     gene            complement(1936..>3576)
                     /locus_tag="PLT69_07050"
     CDS             complement(1936..>3576)
                     /locus_tag="PLT69_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ14230.1"
                     /translation="EVCRQVPAGPARNLHEALQFFIFITFIVNYIDQPQVGNGIRFDK
                     VMGPFLEKDLQDGALDRARAKELVEAVWVKMQEGGYVQPPQWVNNGGGGLGFQTITLG
                     GTDELGNDITNEMTYLALEVTGELQTIAPQIAVRIHDGTPAGLYRAILKCARTGCAQP
                     AVFNDKVNVPRLVELGCPLEEARLYSINNCMQPVIPGKNCHYRAGHSGGLVLPMCLNL
                     ALDEGRFNGKVVGAPTPPVSSMRSFEDVWRAFSAQVEFAAETLGIISNIADVLMKEYI
                     PRPFLSAVLDGNIEHGRDMRDWEYLGLRHHLVFGCNNAADGLAAIKKLVFEEGKLTLE
                     EFVEAVRNNYEGKHERVRLMIERDAPKFGNDDDYVDLISRDIMRLVRAQCDRWTDIYG
                     KPYILDGTTASAPIGAGMILPATFDGRKAGEPFHDGSISPVQGRDTSGPTATIRSVAK
                     VKPLETGNFLLNQKFMPAFFDPEHYDVMEGYLKTLKDLGIHHVQFNCVDARTLIDARE
                     NPEKYRDLIVRVAGYSAYFIDLDRAMQDEIIRRTEQAF"
BASE COUNT          559 a         1186 c         1179 g          652 t
ORIGIN      
        1 gtacgcctcg acgaactcgc gggggatgtc ctcatagggg tagaagtacc aggaacgcct
       61 ctgcctcgcc tcctcctcgc tcaccgtcgg cgccgcgaag aagaacctgc cgtctttctc
      121 gacgagcgtg tagatccact tgaagtcggc gtcgcggcag aagccgttga ccgccctccg
      181 gttgcgcagc tcctcgtcga agggaatcga tcccgcatcc acggccctgt cgtggaagtc
      241 gggcgcgagc atgtacttca ggccccgggc cgccatgaga agacgcctgt cgatctcccc
      301 ctggaattgt gtgacccgct cgtggttcgc ccacaccgcg taggcgacga cgcacgccgc
      361 atacaggcag aggctggcga tcccgtaggc gaggtgtctc ccctggccct tctcgctcac
      421 ggcatacccc cttggtggac gtgatgacag actcacgtgt catacaccat gggggcgact
      481 cggtaaagcc ggcgtggcga cccgggcgcg ggcggggacg tgccgggggt gaccggaggc
      541 ggcgaggttt gcatccaggg tcggcagcct cgatgataca atccctctgt gacgggcggg
      601 tgatcggtac gcgaccgggc aggcggacgc atcttgggcg ggttcgagct gcgatcaccc
      661 ccgaggcccg tgcatgcgcg agcccgtcgg gcatgagcat cttcgagccc ccacgccaag
      721 ccgggaagac gctctcgccc atgcacgtgt cgatcccttc aagaaacggt gaccagccta
      781 tccgatgggt gtctccggcc ggagcggggc tcgggctcgg cccttggcgc cccgaggaag
      841 acggtgcgtc ggcccttgcc ggcagagcgc gggagtcacg aggcccacga cacgggggac
      901 acccctgggc ggccgtgcat gaaccgcgat tcccgggggt atccctgcgc cccgcatgaa
      961 caacgggcgg cctcaggact tctcgatgag gacgaagccg tccatgtgga ggtagcgcct
     1021 cctgagcgct tcgagcatct cgggggacac gtcctcgaac gccggatacg ggttgggcat
     1081 gccgaggcac tcgtacttgt gctctcccca ccggtgcagg gggaggaacc agcaccgctt
     1141 tgcgccgacc tcgcgggcca tcgagacgat ctcgtcggtg acctcggggc cgtcgttgta
     1201 cccggggacc agcggcgtcc gggtccaaat ggcgacctgc ccggcgagcc tgcggaggtt
     1261 ctccaggatg agcgcgttgt cggcatgtgt ggcctccaca tgcctcgccg ggtccgggtg
     1321 cttgatgtcg aacagcacga ggtccacgtg cgggagcacc tcctcgaacg cctcccacgg
     1381 cgagcatccg gacgtgtcga gcgcggtctc tatgccgagc cggtggagcc cctcgagcac
     1441 acaacgggtg aacgcgggct gggagagcgg ctccccgccc gacaccgtca cacccccgcc
     1501 ggtgtgctcg tagaagggac ggtctttgac gagcctctcc acgaggcttt gggcgtctat
     1561 cacggtgccg accgtcgata tgcagccgtt gacgcacgcg tccacgcaca ccaggcaatc
     1621 gtcgcacccc ctgcggtcga acctcacgcg gccgcccacc aggctcaggg cccccctcgg
     1681 gcatgccgcc acgcaacggc cgcagccttc acaccgatga ggcgttaccc gtatctggac
     1741 ggcgctggag atcgactcgg ggttggagca ccaggcgcac ctcaagggac agcccttgag
     1801 gaagacggtg gtgcgtatgc ccgggccgtc gtggatgctg aaatgctgta tgttgaaaat
     1861 cgtccccttc gcctcgcgcg ggttcatgtc catggccggc tttcccccgc gcccggtctc
     1921 cgggcgcggg tcgcttcaga acgcctgctc ggtgcgcctg atgatctcgt cctgcatggc
     1981 cctgtcgagg tcgatgaagt acgcggagta gccggcgaca cgcacgatga gatcgcggta
     2041 cttttccggg ttctccctcg catcgatgag cgtcctcgcg tcgacgcagt tgaactggac
     2101 gtggtggatg cccaggtcct tgagcgtctt caggtagccc tccatgacat cgtagtgctc
     2161 cgggtcgaag aatgccggca tgaacttctg gttgagcagg aagttgcccg tctcgagcgg
     2221 cttcaccttg gcgacggatc gtatggttgc ggtcgggccc gaggtgtccc tgccctggac
     2281 gggcgatatg ctgccgtcgt ggaagggttc ccctgccttc cgtccgtcga aggtcgcggg
     2341 caggatcatg ccggcgccga tgggcgcgga tgccgtggtg ccgtcgagga tgtacggctt
     2401 gccgtagatg tccgtccacc tatcgcactg ggcgcgcacc aggcgcatga tatccctgct
     2461 gatgagatcc acgtagtcgt cgtcgttgcc gaacttcggg gcgtcgcgct cgatcatgag
     2521 gcgcaccctc tcgtgcttcc cctcgtaatt gttccggaca gcctccacga actcctcgag
     2581 ggtcagtttc ccctcctcga agacgagttt cttgatcgcg gcgagcccgt cggccgcgtt
     2641 gttgcacccg aagaccaggt ggtgcctgag gccgaggtat tcccagtcgc gcatgtccct
     2701 gccgtgctct atgttcccgt ccaggacggc cgagaggaac ggcctcggga tgtactcctt
     2761 catgagcacg tccgcgatgt tggatatgat gcccagggtc tccgcggcga actccacctg
     2821 ggccgagaag gccctccaca cgtcctcgaa gctcctcatg gaggagacgg gcggcgtcgg
     2881 ggcgccgacg accttgccgt tgaacctccc ctcgtcgagc gccaggttca ggcacatggg
     2941 gagaacgaga ccgccgctgt ggccggcgcg gtagtggcag ttcttgcccg ggatgacggg
     3001 ctgcatgcag ttgttgatcg agtagagcct cgcctcctcc agggggcagc cgagctccac
     3061 gagcctcggc acgttcacct tgtcgttgaa cacggcgggc tgggcgcacc ccgtccgtgc
     3121 gcacttgagg attgcacggt agagccccgc cggggtcccg tcatggatgc gcacggcgat
     3181 ctggggcgct atggtctgca gctccccggt gacctcgagg gcgagatagg tcatctcgtt
     3241 ggtgatgtcg tttcccagct cgtccgtgcc gcccagggtg atggtctgga agccgagccc
     3301 tccgccgccg ttgttcaccc attgcggagg ctgcacgtac cccccctcct gcatcttcac
     3361 ccagaccgcc tcgacgagct ccttcgccct cgccctgtcg agggccccgt cctggaggtc
     3421 cttctccaag aacgggccca tgaccttgtc gaaccggatg ccgttgccca cctgcggctg
     3481 gtctatgtag ttgacgatga acgtgatgaa gatgaagaac tggagcgcct cgtgcaggtt
     3541 cctcgcaggc ccggcgggta cctggcggca gacctc
//
LOCUS       DAOSCR010000250         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16621_length_3574_cov_3.118215, whole genome shotgun sequence.
ACCESSION   DAOSCR010000250 DAOSCR010000000
VERSION     DAOSCR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16621_length_3574_cov_3.118215"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLT69_07055"
     CDS             complement(<1..248)
                     /locus_tag="PLT69_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ14231.1"
                     /translation="MKPEDKPLISVVIPTYNRAELLEKSLESLTAQSLPKGRFEVIVI
                     DDGSPDNTREVCEGFADRLTLRYQRQENSGGLPQHADER"
     gene            complement(245..3514)
                     /locus_tag="PLT69_07060"
     CDS             complement(245..3514)
                     /locus_tag="PLT69_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ14232.1"
                     /translation="MSGFGDPLARPDTRALLMDEELGHLKFDLITNALLLPRFWDRIR
                     HQNFGDLLVSIDAADKKTYETIRRGGVWEDLLESLALIEKNREKFDSITLNMTVMREN
                     YRSIPAFIDLALSRGFKASFQRIRGPYPWQNIFDPPDEDALRTLQGIVEHELAKRDTS
                     RVFFGDLLVFNEHTPGSTEAPGQEALRLFEQGMLAEAIAAMERAVANDPDNAVLHNDL
                     GAMYHASGEASKARACFEKALDLDPTYAPSLKNLTELFMEMGRPDDARATCSTLISLH
                     PADEEAGRLWAVINGGIEGGAPVGNGAIQDTANTCEDTPNSIRKNREQWSNYEWTEAG
                     DEWSACWGGTGQLWERTIKPRISSFLPSGHILEIAPGFGRCTQYLVPECSELTLVDLT
                     EKCIEACRKRFQAHSHIRCFVNDGKSLDMLEDDSIDFAFSWDSLVHVEKDVMRSYLHE
                     LSAKLKPGGAAFLHHSNMGAFLDPTTGKLTVKNDHWRGEDMSAKLFRDFCLEAGLKCV
                     SQEILAWGGDVLNDCFSVVVKEPGYDPDKTVVVENPDFMDESKGRKNPPAIYGAGCLP
                     GSGQAAFEHGEAKGKPGGDAPEAVRTPCTLSIVMCVSEDADRAVRSVKSLLRGLPRHG
                     VELIVAAPAGGGQASDRLERLQNDGLTLVIPEGPCTEVNRCVTAAKKASGRFLALTDD
                     SVLFSKTWLPEIMKAIDESDGFDALVGKTVTVDGLIVEAGSSSPGKDWLEGRGEGSVL
                     HDPAYSFSCLASSGSRYAMLVSRRAWDDMDGLDEGLEHLGCALVDLGLRMVSRGFGIL
                     YQPQCVLVANDGVSSRAQEPASLSLAPGRLASLRPRELAPGILQAVSGSGRRRVLVLG
                     IYLANTLNTVTDLVGVFSRSRFHDVTQKWVALNGPAPDAATARVTVRELTGRFPKFQI
                     VNDLLAGEDLKGYDYVILCDDDVVLPEGFVDSFVGLQERLGFAVAQPARTLNSYIDHP
                     IVQQHIGVHARQTLFVEIGPVVSFHRSSFDFVFPFDLTSPMGWGYENVWAREVLTRGL
                     KMGIIDAVCVDHSIRKPVANYDWSQADEQRNAYLAGNPHLALEDCFTVLEAHLIAEER
                     V"
BASE COUNT          578 a         1127 c         1190 g          679 t
ORIGIN      
        1 cgctcatcag cgtgttgtgg aaggccgccg gagttttcct ggcgctggta gcgaagcgtc
       61 agcctgtccg cgaagccttc gcatacctcc ctggtgttgt cgggcgaccc gtcgtcgatg
      121 acgatgacct cgaaccggcc tttcgggaga ctctgggcgg tcaggctctc gagggacttc
      181 tcgagcagct ccgcccggtt gtacgtgggg atgacgacgc tgatgagtgg cttgtcttca
      241 ggtttcatac ccgctcctct gcgatgaggt gtgcctcgag gaccgtgaag cagtcttcga
      301 gcgcaaggtg ggggttgccg gcgaggtagg cgttcctctg ctcgtccgcc tgggaccagt
      361 cataattggc caccggcttg cggatgctgt ggtccacgca caccgcgtcg atgatgccca
      421 tcttcaggcc ccgcgtgagg acctcgcggg cccacacgtt ctcgtagccc caccccatgg
      481 gcgaggtgag gtcgaagggg aacacgaagt cgaaggacga tctgtggaag ctcaccaccg
      541 ggccgatctc cacgaacagg gtctggcgcg cgtggacccc gatgtgctgc tggacgatgg
      601 ggtggtcgat gtaggagttc agcgtgcggg ccggctgcgc gacggcgaag ccgagccttt
      661 cctgcaggcc gacgaacgag tccacgaacc cctcgggcag gaccacgtcg tcgtcgcaca
      721 ggatgacgta gtcgtatcct ttcaggtcct ccccggccag gaggtcgttt acgatctgga
      781 acttggggaa cctcccggtg agctccctca ccgtcacccg ggccgtcgcg gcatccgggg
      841 cgggcccgtt cagggccacc cacttctgcg tcacgtcgtg gaagcgggag cggctgaaca
      901 cccccacgag gtcggtcacg gtgttcaggg tgttcgccag gtagatgccc aggacgagca
      961 ccctcctccg tcctgacccg gataccgcct ggaggatccc gggtgcgagc tccctcggcc
     1021 tcagggaggc cagcctcccc ggggcgagag acagtgatgc gggttcctgt gcgcgggagg
     1081 aaaccccgtc gttcgcaacg aggacgcact ggggctggta gaggatgccg aaccctcgcg
     1141 agaccatcct gagcccgagg tccaccaggg cgcaccccag gtgttcgagg ccctcatcga
     1201 ggccgtccat gtcgtcccag gcccgcctgg acacgagcat ggcgtaccgg gaccccgagc
     1261 tcgcgaggca ggagaaggag tatgcggggt cgtgcaggac agagccttcg ccccttcctt
     1321 caagccagtc tttgcccggc gacgaggagc cggcctccac gatcaggccg tccacggtga
     1381 cggtcttgcc caccagggcg tcaaagccgt ccgactcgtc gatggccttc atgatctccg
     1441 gaagccaggt cttgctgaag agcaccgagt cgtccgtcag ggcgaggaac ctgccggagg
     1501 ccttcttcgc cgccgtgacg cagcggttga cctcggtgca tggcccctcg ggaatgacca
     1561 gggtgaggcc atcgttttga agcctttcca acctgtcgga ggcttggccg cctccggctg
     1621 gggccgccac gatgagttcc accccgtgcc tggggaggcc cctgaggagg gacttgacgc
     1681 tgcgcacggc acggtcagcg tcttcactca cgcacatgac gatggagagc gtgcatgggg
     1741 tgcggacggc ttcaggcgcg tccccacccg gcttcccctt tgcctccccg tgctcgaagg
     1801 ccgcctgccc gcttccggga agacacccgg caccgtagat ggccggtggg ttcttccgcc
     1861 ccttcgactc atccatgaaa tccgggttct ccaccaccac ggtcttgtcc gggtcgtagc
     1921 ccggctcctt cacgaccacg gagaagcagt cgttcagcac gtcgccgccc caggcgagga
     1981 tttcctggga cacgcacttc aggcccgcct ccaggcagaa gtccctgaac agtttcgccg
     2041 acatgtcctc gccccgccag tgatcgttct tcacggtgag tttgccggtg gtcgggtcca
     2101 ggaacgcgcc catgttggag tggtggagga aggccgcgcc accgggcttg agcttggccg
     2161 agagctcgtg gaggtaggag cgcatgacgt ctttctccac gtggaccagg gagtcccagc
     2221 tgaaggcgaa gtcgatggag tcgtcttcca gcatgtccag ggacttgccg tcgttcacga
     2281 agcagcggat gtgggagtgg gcctggaaac gcttcctgca cgcctcgatg cacttctccg
     2341 tgaggtccac gagcgtgagc tcggagcact ccgggacgag gtactgggtg cacctcccga
     2401 accccggggc gatctccagg atgtgccccg acggcaggaa ggaggagatg cggggcttga
     2461 tcgtcctctc ccagagctgc cctgtgcccc cccagcatgc cgaccactcg tcgcctgcct
     2521 cggtccactc gtagttcgac cactgttccc tgttcttcct gatggagttc ggggtgtcct
     2581 cgcaggtgtt cgctgtgtct tggatcgcgc cgttgccgac aggtgcccca ccctcgatgc
     2641 cgccgttgat cacagcccac aggcgacccg cctcctcgtc ggccggatgg agcgatatca
     2701 gcgtgctgca ggttgcgcgc gcgtcatcag gcctacccat ctccatgaag agctcggtga
     2761 ggttcttgag cgagggggcg tatgtggggt cgaggtcgag ggccttctcg aagcatgcac
     2821 gggcttttga cgcctcgccg gatgcgtggt acatcgcccc caggtcgttg tgaagcacgg
     2881 cgttatccgg gtcattggca acggcgcgct ccatggcggc gatggcctct gccagcatgc
     2941 cctgctcgaa gagcctgagc gcctcctgac cgggcgcctc ggttgatccc ggggtatgtt
     3001 cgttaaagac gagcagatcg ccgaagaaga ctcgggatgt gtcccgcttg gcgagttcat
     3061 gttccacgat gccctgcagg gtgcgcaggg catcctcatc gggcgggtcg aagatgttct
     3121 gccaggggta gggcccgcgg atacgctgga acgaggcctt gaaccccctg gacagggcga
     3181 gatcgatgaa tgccgggatg gacctgtagt tctcgcgcat caccgtcatg ttcagggtta
     3241 tggaatcgaa tttctcccgg ttcttttcga tgagggcaag gctttccagg aggtcttccc
     3301 agacaccgcc cctgcggatc gtctcgtagg tcttcttgtc agcggcatcg atggagacca
     3361 ggagatcgcc gaagttctga tggcggatgc ggtcccagaa tcggggaagg agcagggcgt
     3421 tggtgatgag atcgaacttc aggtggccga gctcttcgtc catgagcagg gcgcgtgtgt
     3481 ccggccgggc gagggggtct ccgaaaccgg acatgacgat cctccgtgca gcgggaaggt
     3541 gtctccgcag gtcgtccgtg gtcttcgcga gcat
//
LOCUS       DAOSCR010000251         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16719_length_3559_cov_3.136130, whole genome shotgun sequence.
ACCESSION   DAOSCR010000251 DAOSCR010000000
VERSION     DAOSCR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_3559_cov_3.136130"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLT69_07065"
     CDS             complement(<1..651)
                     /locus_tag="PLT69_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOJ14233.1"
                     /translation="MEVFTRYEGNPILTVKDIPYPANSVFNAAACVFGSQTLLLMRVE
                     DKRGISHLTVATSDDGVTGWRVEPGPALEPDPVKHPEEIWGIEDPRITRIDDLDLWAV
                     LFTAYSRGGPLVSLATTRDFRVFERKGVVMPPEDKDAALFPVRFGGRWAMVHRPASSV
                     EHTGAHIWISFSPDMKHWGDHQVLLPARKGAWWDANKIGLSPPPMETDTGWLVLYHG"
     gene            complement(825..2051)
                     /locus_tag="PLT69_07070"
     CDS             complement(825..2051)
                     /locus_tag="PLT69_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ14234.1"
                     /translation="MARKPIVMVSSYPPRLCGIATFCEEAREFIQKANPDRDVLVISH
                     HDGSGDGVFPVIDTSRPDWWRTVAKTIERLDPYVVHIEHEYGLYEYRDERGMGDGNDG
                     FLDLLEAIAPYPVVVEPHTVHGRLSDFEADFIFRLCQRAHVVLFKCHYQKWRLDWTFH
                     TRGWATPRNIMVVPHGARPDKRWGVHEIPELRKEFGLDDMGLAGHLVGMVGWIQSNKR
                     WDILLSMWEDIHHEILRRTGQEWDLLAAGTMRDAHHRKDYEEWKSDLVALKAKGIAHY
                     HEFVPRGDDYYKIMALCDFIVLPSTDETQSGTLARIIALNKPYITTAPMEGLTAQTLE
                     SEGGLLFTTKEMLRQKVIKLACDENLRLELGQNLKRYLDEVVSWEVVAAQYNEAYRLA
                     RWSSRTGRPVVMELEF"
     gene            complement(2059..>3559)
                     /locus_tag="PLT69_07075"
     CDS             complement(2059..>3559)
                     /locus_tag="PLT69_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ14235.1"
                     /translation="RFVDLQELCEFLGAADVYVTPYLQKEQITSGTLAYALGSGKATV
                     STPYWYAEEMLADGRGRLVPFKDPAALAERVNDLLSNEVERHAMRKRAYTFCREMVWK
                     EVARRYLEVFAEVKQERERSPKPVYYAKTVGASPSEIPQPKLDHIVRLTDDVGILQHA
                     KYIVPDRFHGYCTDDNARALIAVLMAREMIPEDEQASLMVPTYLAFLFHAFNEENSRF
                     RNFMGYDRRWLEDKGSEDSHARALWALGEAVALAWTDDIRAVAKHLFEKALPVVTDFS
                     SPRAWAFALIGIASYLRMFAGDSEVRRVRERLAEMLLGLYRQTACDDWPWIEDRLAYA
                     NGKIPQALILTGRSLENDEMLQAGIESLDWLYREQTDPKGHFVPVGNNGWYEKGGVKA
                     RFDQQPVEVYHMIEACREAYSATGDEIWVTRAQCCLEWFLGRNDLNVPLYDHKTGGCC
                     DGLQAGGPNRNQGAESTLACFLSLLSINRFGSLGLELARNGSREERPTE"
BASE COUNT          562 a         1199 c         1101 g          697 t
ORIGIN      
        1 cccgtggtag agcacgagcc accccgtgtc cgtctccatg ggaggggggc tgaggccgat
       61 cttgttcgcg tcccaccacg cccccttccg tgcgggcagg aggacctggt ggtcgcccca
      121 gtgcttcatg tcgggactga acgagatcca gatgtgtgcg ccggtgtgct cgaccgagga
      181 cgcgggcctg tgcaccatgg cccagcgtcc gccgaaacgc acgggaaaga gcgccgcgtc
      241 cttgtcctcc gggggcatga ccaccccctt gcgctcgaac accctgaagt ccctcgtggt
      301 cgcaagcgag acgagggggc cgcccctgga gtaggccgtg aacagtacgg cccagagatc
      361 gagatcatcg atgcgcgtga ttctggggtc ctcgataccc cagatctcct cggggtgctt
      421 cacggggtcc ggctcgaggg ccgggcccgg ctccacccgc caacccgtga cgccgtcgtc
      481 gcttgtggcc accgtgaggt gggagatgcc cctcttgtcc tccacgcgca tgagcaggag
      541 cgtctgtgag ccaaagacgc aggctgccgc gttgaagacc gagttggcag ggtacgggat
      601 gtccttcacc gtcaggatcg ggttcccctc atacctcgta aagacttcca tgccgtctcc
      661 tcctgtgtcc tgttgccggc cggacgagcg tggcggacgt caagccgccc cagggatcac
      721 acggaccgcc ctcttccccc gctgaagaag cgccctgacc ggcgccgcac ggacaacggg
      781 caagggcgaa gccatgggcc gccggggccg gccccttgcc gaggtcagaa ttcgagttcc
      841 atgaccaccg gcctgcccgt tctcgacgac catctggcga gcctgtatgc ctcgttgtac
      901 tgggccgcga cgacctccca actcaccacc tcgtccaggt atctcttcag gttctgcccg
      961 agctccagcc tcaggttctc gtcgcatgcg agcttgatca ccttttggcg gagcatttcc
     1021 ttcgtcgtga agagcaggcc gccctcgctc tcgagcgtct gcgcggtcag cccttccatg
     1081 ggggccgttg tgatgtaggg cttgttcagg gcgatgatcc ttgccagcgt ccccgactgc
     1141 gtctcgtccg tcgacggaag gacgatgaaa tcgcacaggg ccatgatctt gtagtaatcg
     1201 tccccgcggg ggacgaactc gtggtagtgc gcgatgccct ttgccttgag cgccacgaga
     1261 tcgctcttcc actcctcgta gtccttcctg tgatgcgcgt ccctcatggt gccggcggcc
     1321 aggaggtccc actcctggcc cgtgcgcctg aggatctcgt ggtggatgtc ctcccacatg
     1381 gagaggagga tgtcccaccg cttgttggac tgtatccagc cgaccatccc gacgaggtgc
     1441 ccggcaagcc ccatgtcgtc caagccgaac tccttcctca attccgggat ctcgtggacg
     1501 ccccacctct tgtcgggtct cgcgccgtgg gggaccacca tgatgttcct cggcgtggcc
     1561 cagcccctcg tgtggaaggt ccagtccagc ctccacttct ggtagtggca cttgaacagg
     1621 accacgtgcg ccctctggca gagcctgaag atgaagtccg cctcgaagtc gctgagcctg
     1681 ccgtggacgg tgtgcggttc cacgacgacg gggtacggcg caatggcctc gagcaggtcg
     1741 agaaacccgt cgttcccgtc gcccattcca cgctcgtccc ggtactcgta gagaccgtac
     1801 tcgtgttcga tgtggaccac gtaggggtcg agcctctcga tcgtcttcgc caccgtcctc
     1861 caccagtcgg gcctgctcgt gtcgatgacc gggaacaccc catcgccgga accgtcgtgg
     1921 tggctgatca cgagcacgtc cctgtcaggg ttcgcctttt ggatgaactc ccgcgcctcc
     1981 tcgcagaagg tggcgatgcc gcagagtcgc ggggggtagg agctcaccat tacgattggt
     2041 tttctcgcca tgggggtctc actccgtcgg tctttcttcg cgtgatccgt tccgggcgag
     2101 ctcgaggccg aggctgccga agcggttgat gctcaggagg gacaggaagc atgcgagggt
     2161 cgactcggcc ccctggttgc ggttcggccc tcccgcctgc agcccgtcac agcatccccc
     2221 ggtcttgtgg tcgtacaggg gcacgttcag gtcgttgcgg ccgaggaacc actcgagaca
     2281 gcactgggcc ctggtgaccc agatctcgtc accggtggcc gagtacgcct ccctgcaggc
     2341 ctcgatcatg tgatacacct cgacgggttg ctggtcgaac ctggccttga cccctccctt
     2401 ctcgtaccaa ccgttgttcc cgacgggcac gaaatggcct ttggggtccg tctgttccct
     2461 gtagagccag tcgaggctct cgatccccgc ctggagcatc tcgtcgtttt cgagcgagcg
     2521 cccggtcagg atgagcgcct gggggatctt gccgttggcg taggcgagcc tgtcctctat
     2581 ccagggccag tcgtcgcagg ccgtttgcct gtagagcccg agcagcatct ccgcgaggcg
     2641 ctcccgcacg cgtcgcacct cgctgtcgcc cgcgaacata cgcaggtagg aggcgatgcc
     2701 gatgagcgcg aaggcccatg ccctgggcga ggaaaagtcc gtcaccaccg gcagggcctt
     2761 ctcgaacagg tgcttggcca cggcgcggat gtcgtcggtc cacgcgagag ccaccgcctc
     2821 gccgagcgcc cacagggccc tggcatggct gtcctcggaa cctttgtctt cgagccacct
     2881 ccgatcgtac cccatgaagt ttctgaaccg cgagttttcc tcgttgaagg catgaaagag
     2941 gaaggcgaga taggtgggga ccatgaggga cgcctgttcg tcctctggta tcatctccct
     3001 tgccatgagc accgcgatga gcgcccgggc gttgtcgtcg gtgcagtacc cgtggaaccg
     3061 gtccgggacg atgtatttcg cgtgctggag gataccgaca tcgtcggtga ggcgaacgat
     3121 gtggtcgagc ttcggctgcg gtatctcgct gggcgaggcc cccaccgtct tggcatagta
     3181 cacgggtttc ggcgatcgtt cccgctcctg tttcacctcc gcgaagacct cgagatacct
     3241 ccgcgccacc tctttccaga ccatctcgcg gcagaaggtg tacgcgcgct tgcgcatcgc
     3301 gtgcctctcc acctcgttcg acaacaggtc gttcaccctc tccgcgagcg cagccgggtc
     3361 cttaaacggc accaacctgc ccctgccgtc ggccagcatc tcttcggcat accagtaagg
     3421 ggtcgacacc gtcgccttcc cgctcccgag cgcgtaggca agcgtaccgg aggtgatctg
     3481 ctccttctgg aggtacgggg tcacgtacac atccgccgca cccaggaact cgcacagttc
     3541 ctgcaggtcc acgaaacgg
//
LOCUS       DAOSCR010000252         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16730_length_3557_cov_4.159623, whole genome shotgun sequence.
ACCESSION   DAOSCR010000252 DAOSCR010000000
VERSION     DAOSCR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16730_length_3557_cov_4.159623"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLT69_07080"
     CDS             complement(<1..238)
                     /locus_tag="PLT69_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14236.1"
                     /translation="MERDLLSILKERIVSLEEGYEQKIAELSLLKELGEALKGSSLAN
                     WNQIFMRQLDIIKRATGIHSISVMLIDEESGNLYV"
     gene            complement(244..1695)
                     /locus_tag="PLT69_07085"
     CDS             complement(244..1695)
                     /locus_tag="PLT69_07085"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ14237.1"
                     /translation="MDLKDILAPLDVELKKVSMLGGSLAVVVVPASHAELAMKAKRET
                     DTALKCGDYLVVGLFGMGADEASTRFLSIPKGIAVYPADAGDIQTLFFEAAKRLNMNT
                     LSAPWVKDVWESIKQTNVQDADREDRFLKGSFYQFDAFVADHPQDMGRMLSFMAPADR
                     RWVEDVLPIGVVSFMEAQSESEDTGQEGGPAGASLIEQWDFKSRFETKVEQARATLRK
                     FRSIEHLFTLPSISHEVIEAASDPMTAAPRMARIIEKDPVLTSRVLKVVNSAFYGFRR
                     QIVSVEHAVVILGNDEVVNLAFSIAVHQIMDRVSPSHGAELWEHSLMVAHLSQWFGGV
                     LGSKASRELYTIGLLHDFGKIVFLQKGYRVPGFAEMSTVHRLAAEEMSMGISHAEMGA
                     YVAERWNLPEEIVDTLLTHHQPGRAKDMMLSASVHLADAVAHRGAIPRDEVNSAVARL
                     LEGADGSRIGDEVVSKRYDYTLKRVKTLLDIDG"
     gene            1835..3073
                     /locus_tag="PLT69_07090"
     CDS             1835..3073
                     /locus_tag="PLT69_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOJ14238.1"
                     /translation="MIPVDQARESILDHVNVLPAERVWFMNALGRFLAEDIVSTRDIP
                     AFDNSAMDGYAVRCEDVPGANTALRITGVLPAGTRAIPRLEAGHAVKIMTGAPVPPGA
                     DAVVRKEDASEQDGVVTIAKAPAKYENIRFAGEDIRKGSLALTAGDHVGPAHVGVLAS
                     LGRLHVMVGQRPRVAVVATGDEIVEPGDEGAGAFAVTSSNSYTLASLISSCGGVPVYL
                     GIARDSEASLEDALDRARLCDLIVTTGGVSMGEFDLVRKIMNAHPNRLHFWGVEIKPG
                     KPLAFGEINGIPTIGLPGNPASTMTAFYQFVRPALLKMQGARDILLPRVQARLAQRVS
                     SKGDRPHYIRCLLECRDEGLVVTPTGPQGSGILSSLARGNCFVVVPKGTRVLERGDLV
                     DCELYDGIRSGSKHEAHAQP"
     gene            3269..>3557
                     /locus_tag="PLT69_07095"
     CDS             3269..>3557
                     /locus_tag="PLT69_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOJ14239.1"
                     /translation="MLRKNELLEIREILVQMKKEILETLSERIKSSDIASQREIGDIF
                     DEADLEQTREFNLLLGTREKQKLEQIESALARMDKGEYGFCEDCGENIPIGR"
BASE COUNT          678 a         1131 c         1100 g          648 t
ORIGIN      
        1 cgacgtagag gttcccgctc tcctcgtcga tgagcatgac ggatatgctg tgtatccctg
       61 tcgccctctt gatgatgtcg agttgcctca tgaagatctg gttccagttg gccaggctgg
      121 agcccttgag cgcctctccg agctccttga gcagcgagag ctcggcgatc ttctgctcgt
      181 acccctcctc gagggacacg atgcgttcct tgaggatcga gaggaggtct ctctccatct
      241 gtcttacccg tcgatatcga gcagggtctt tacccgcttc agggtgtagt cgtacctctt
      301 ggaaacgacc tcgtcgccga ttcgtgagcc gtccgcgcct tccaagaggc gcgcaacggc
      361 gctgttgacc tcgtcgcgag ggatcgcccc ccggtgggcc accgcatccg ccagatgaac
      421 ggaggcgctg agcatcatgt ccttcgccct gcccggctgg tggtgggtca acagggtgtc
      481 cacgatctcc tcaggcaggt tccaccgttc tgcaacgtat gcgcccatct ccgcatggga
      541 gatccccatc gacatctcct cggccgcaag ccggtggacg gtggacatct cggcaaagcc
      601 cggcaccctg tatcccttct ggaggaagac gatctttccg aaatcgtgaa ggagccctat
      661 cgtgtagagc tctctcgacg ccttggaccc caggacgccg ccgaaccact gagaaaggtg
      721 ggcgaccatc agcgagtgtt cccagagctc ggccccgtgg ctgggagaca ccctgtccat
      781 gatctggtgg accgcgatgg aaaacgcgag attcaccacc tcgtcgtttc ccaggatcac
      841 caccgcgtgt tccaccgaca cgatctgcct gcggaacccg tagaaggcgg aattcacgac
      901 cttcagaacg cgcgacgtga gcacgggatc cttctctatg atcctcgcca tccttggggc
      961 cgctgtcatg gggtcggatg cggcctcgat gacctcatgg gatatggagg gcagcgtgaa
     1021 caggtgctct atgctcctga acttcctgag ggttgcccgc gcctgctcca ccttcgtctc
     1081 gaagcgggac ttgaagtccc actgctcaat gagggaagcc cctgcgggac ctccttcctg
     1141 ccccgtgtcc tcgctctccg actgggcctc catgaacgac acgaccccta tcgggaggac
     1201 atcctcgacc cagcgcctgt cagccggggc catgaaagag agcatcctcc ccatgtcctg
     1261 gggatggtct gcaacgaacg cgtcgaactg gtagaaggac cccttcagga agcggtcctc
     1321 cctgtccgcg tcctggacat tggtctgctt gatgctctcc cacacgtcct tgacccacgg
     1381 ggcgctgagc gtgttcatgt tgagccgctt ggcggcctcg aagaagagcg tctgtatatc
     1441 cccggcgtca gccgggtaca ccgctatacc cttggggatc gacaggaagc gggttgatgc
     1501 ttcatcggcc cccatgccga acaagccgac caccagataa tcgccgcact tgagcgccgt
     1561 gtcggtttcc cgtttggcct tcatggccaa ctccgcgtgg ctcgcgggta ccacgaccac
     1621 ggccaggctc ccgcccagca tggatacctt cttgagctcg acgtccaaag gcgcaaggat
     1681 atccttcaaa tccatgggta ttctcttcga catcgcagca tcaaatcttg acttggccgg
     1741 cgggctccct ttaggatacc ttctcggtca agagacgccg ggagaaccga cgccaagacg
     1801 gcgcctggac gccggaaacg aaagggagaa acggatgatc cccgttgatc aggcccggga
     1861 aagcatcctc gatcacgtca acgtcttgcc ggcggaacgg gtgtggttca tgaacgcgct
     1921 cggcaggttc ctcgcggagg atatcgtgtc cactcgggat atcccggcct tcgacaactc
     1981 ggccatggac gggtacgccg tccgttgcga agacgtgccg ggggcgaaca cggcccttcg
     2041 catcacaggg gtcctgcccg ccggcacccg ggccatcccg aggctcgagg ccgggcatgc
     2101 cgtgaagatc atgaccgggg cgcccgtgcc tccaggcgcg gacgcagtgg tcaggaagga
     2161 agacgcatcc gaacaggatg gggtggtgac catcgcaaag gcgccggcga aatacgagaa
     2221 tatcaggttc gcaggcgaag acatccgcaa ggggagcctc gcccttacgg ccggggatca
     2281 cgtcggcccc gcccacgtcg gggtgcttgc gtcccttggc aggctccacg tgatggtcgg
     2341 ccagaggccc agggtggccg tggtcgccac cggtgacgag atcgtcgagc ccggcgatga
     2401 gggagcaggc gcctttgccg taacctcgtc gaactcgtac acgctcgcgt ccctcatctc
     2461 gtcttgcggc ggcgtcccgg tttacctggg gatcgcccgg gacagcgagg catccctgga
     2521 agacgccctc gacagggcga ggctgtgcga tctcatcgtc acgaccggcg gcgtttccat
     2581 gggcgagttc gacctggtga ggaagatcat gaacgcacac cccaaccgtc tccacttctg
     2641 gggcgtggag atcaagcccg ggaaaccgct cgccttcggt gagatcaacg gcatccccac
     2701 gatcggcctc ccgggcaacc cggcctccac catgaccgcc ttctaccagt tcgtgaggcc
     2761 cgccctgctc aagatgcagg gcgcacggga catcctcctc ccgagggtgc aggcgcgcct
     2821 ggcgcagagg gtctcgagca agggagaccg tccccactac atccgctgtc tcctggagtg
     2881 cagggacgag ggtctcgtgg tgacacccac gggcccccag gggtcgggga tcctctcctc
     2941 gttggccagg ggcaactgct tcgtggtggt acccaaggga acgagggtct tggaacgcgg
     3001 agacctcgtg gactgcgagc tgtacgacgg gatccggtcg ggctcgaagc acgaggccca
     3061 cgcgcaaccc tgagttgttg caacttcggc ctgtgcccgc aactccccgc gacacggccg
     3121 agtcatccgg tcttccccca aggagcggat ctctgcgcgg cgacgctcgg gggcgacgcg
     3181 gggggcgggg acgacggggc gattgatggt tgacatcccc gggaaaatga gtataaaagc
     3241 gatctttcaa tttcctctgg aggacgggat gttacgaaag aacgagctcc tcgagatcag
     3301 ggaaatcctg gtgcagatga agaaggagat cctcgagacg ctgtcagagc ggataaaatc
     3361 cagcgatata gcctcccagc gagagatcgg ggacatcttc gacgaggcgg accttgaaca
     3421 gacacgtgaa ttcaacctgc tgctcgggac ccgcgagaaa caaaagctcg aacagatcga
     3481 gagcgccctc gccaggatgg acaaaggcga atacggcttc tgcgaagact gcggggagaa
     3541 catcccgata ggcaggc
//
LOCUS       DAOSCR010000253         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16889_length_3534_cov_3.508767, whole genome shotgun sequence.
ACCESSION   DAOSCR010000253 DAOSCR010000000
VERSION     DAOSCR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16889_length_3534_cov_3.508767"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLT69_07100"
     CDS             complement(<1..610)
                     /locus_tag="PLT69_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14240.1"
                     /translation="MGGCLVLFMVMIALSPAAAWSFDPSDPLMRVGAGSGAADAGLKG
                     GEGPRSSPAADLERTKSREELFMEVFKHPPPSVPLEMTLPLFVQGNPCGQTRAVLQAD
                     GTDLRLPFAFVIPCLGEIVKPEVIDALGATADPSGMISTAALEDAGLVVVFDKRRLCV
                     SITVPDDLMGTRVHRLSGVRPGPDPSSAMKPSPLSGFVTVNAR"
     gene            complement(683..1483)
                     /locus_tag="PLT69_07105"
     CDS             complement(683..1483)
                     /locus_tag="PLT69_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HOJ14241.1"
                     /translation="MRFRMLCIFMGFMGILSAFVAAPPVHAGFSVSPQIVTLEPSGRR
                     SQQWVTVRHANDDDLPVAVELSILKREMDETGRIHHATEESDDGFVLYPAQMILYPGE
                     TQVVFIQWIKGAAVDKELVFSLVAQSVPIDPGAQKGGEEERGGVRLELRTIVRYEGLI
                     FVRPQKARPDVVVESASVLVDGTPTTMLDLVLLNRGSARQKLKGMTLTVASVGTDGSP
                     GGSAVTYRPRLPEDLTKHSLYAGYRRRFVLPWPEGVPVGPVKVTAAFE"
     gene            complement(1547..2110)
                     /locus_tag="PLT69_07110"
     CDS             complement(1547..2110)
                     /locus_tag="PLT69_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14242.1"
                     /translation="MKIGRTLAATAILPLLLAGQAFADTVYPSGTLSAINSVSSTAVT
                     DLDFDNDGNDVTIATITIDNNYENAFDLTLEFANDGVFKRVAAAGGDGTAATGVGAEI
                     PITEFRLHPNGTGTLGTGLTPPSGTFTLTSDAGADYYVWDTHATQSTATVGYQVEVQA
                     DWSTDTRLLQGTYRETLTVTLSVGDGS"
     assembly_gap    2449..2458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2834..>3534
                     /locus_tag="PLT69_07115"
     CDS             2834..>3534
                     /locus_tag="PLT69_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOJ14243.1"
                     /translation="MGNFKEYDLYDGIGLAELVKKGLVSAAEVLEEAIERIERVNPKI
                     NAVILRMYDSARRAVSSRLPEGPFTGVPFLLKDLLTACAGVPLSMGCRAYRDYVPPVD
                     SELMRRYRASGVVVAGKTNTPEFGLVAVTEPELFGPTRNPWDLGRTPGGSSGGSAAAV
                     ASGMVPMASGGDGGGSIRIPASHCGLFGLKPTRGRTPTGPYYGEIWQGAAVEHVITRT
                     VRDSAAMLDAIHGADP"
BASE COUNT          601 a         1145 c         1132 g          646 t
ORIGIN      
        1 gcctggcgtt caccgtgacg aacccggaca acgggctcgg cttcatcgcg cttgagggat
       61 ccgggccggg ccgaacgccg ctcaaacgat gaaccctcgt gcccatgagg tcgtcaggca
      121 ccgtgatgga cacacacagc cgcctcttgt cgaacacgac aaccaggccg gcatcttcca
      181 gcgccgccgt cgagatcatg ccggatggat cggccgttgc gccgagcgcg tcgatgacct
      241 cgggcttcac gatctcgcca agacacggga tcacgaacgc aaacgggaga cggaggtcgg
      301 tcccgtccgc ttgcagcacg gccctcgtct gaccgcaggg gttgccctga acgaacaggg
      361 gcagggtcat ctccagggga acgctcggcg gcggatgctt gaagacctcc atgaagagct
      421 cttccctgct cttcgtcctc tcgagatccg ctgccgggga cgaccggggc ccttcgcccc
      481 ccttgagacc cgcatcggca gccccgcttc ccgcccccac gcgcatcaag gggtccgagg
      541 ggtcgaacga ccatgcagcg gcaggggaaa gggcgatcat gaccatgaac aggaccaggc
      601 atccccccat gccacccctg ctccccttca aacgtgcatg gtctctcttt cgctcgatat
      661 cccgtcttct ctttcccgtg atctactcga aggccgccgt caccttgaca ggccccacag
      721 gcaccccttc cggccacggc aggacgaacc tcctccgata cccggcatag aggctgtgct
      781 tcgtcaggtc ttcaggaagc cgcggccggt acgtcaccgc actgccaccg ggcgagccgt
      841 cggtgccgac agaggccacg gtgagggtca tccccttgag cttctgcctc gcagaccccc
      901 tgttgagcag cacgaggtcc aacatcgtag tcggcgtgcc gtccacgagc accgatgccg
      961 actccacgac cacatccggt ctcgccttct gtggacggac gaagatcaat ccctcgtacc
     1021 tcacgatggt cctgagctcg agccgtaccc cgcccctttc ctcttcaccg cctttctgcg
     1081 cgcccggatc gatcgggacg ctctgtgcga ccaggctgaa gacgagctcc ttgtcaacag
     1141 ccgcaccctt tatccactgg atgaacacga cctgggtttc cccggggtag aggatcatct
     1201 gggcagggta gagcacgaag ccgtcgtcac tctcttcggt tgcgtggtgg atacggcccg
     1261 tctcgtccat ctccctcttg aggatgctca gctctacggc cacggggaga tcgtcgtcgt
     1321 tcgcgtgcct gaccgtcacc cactgctggg atctcctccc tgaaggctcg agggtgacga
     1381 tctgcgggga gacggagaaa cccgcgtgca cggggggcgc cgccacgaag gccgagagaa
     1441 tccccatgaa ccccatgaat atgcagagca ttctgaatcg cataacccat tccccgcgga
     1501 aggggaggcg tcgtcccctt ccaggaccga tacgccctca tgcatgtcag cttccgtcac
     1561 ccacggagag ggtcacggtg agcgtctccc gataggtgcc ctggagcagc ctggtgtccg
     1621 tcgaccagtc cgcctggacc tccacctggt agcccacagt cgccgtggac tgggtggcgt
     1681 gcgtgtccca gacgtagtag tcggcgccgg cgtccgaggt cagagtgaac gtgccggagg
     1741 gtggggtgag ccctgtcccg agcgtccctg tcccgttcgg atggaggcgg aactccgtga
     1801 tcgggatctc cgcgccgacc cctgtggcgg ctgtcccgtc gccaccggct gcggccaccc
     1861 tcttgaacac cccgtcgttg gcgaattcga gcgtgaggtc gaatgcgttc tcgtagttgt
     1921 tgtctatggt gatggtggcg atggttacgt cgttgccgtc gttgtcgaag tccaggtcgg
     1981 tcacggcggt cgagctgacg ctgtttatcg ccgacagcgt tcctgaagga tacaccgtgt
     2041 cagcaaaggc ctgccctgca agcaaaagcg ggagtatggc tgttgcggcc agcgttcttc
     2101 caatcttcat gatcgttgcc cccttgtggt gtatttcgac ctcttttttc ctttcgagaa
     2161 agcctttccc aaatacctgt atatttatcg gatacacttt gattttgcat aaatgtgagg
     2221 ggctcgatcc aggatggggg catgcatcaa gcgaggtcaa ggcacagggg ggaagcatcg
     2281 gggcgttccc tccgggggca gcgccacgtg cagagatcgg gccgcccctg tccaggctgg
     2341 atcatgtggt tgtaccggga tgaagggctt gcctcgaggg aggacctctt cacgggtcgc
     2401 tttcgggggt cgcatcacgg atcctgggcc gtggaccctt ctgcccttnn nnnnnnnncc
     2461 gggagccctg ggtgtgcctc ggcggagggg cacgtcgtcc ttcccgtgcc tcgagggtct
     2521 ggacaggcag aggcctgggt gctcatcgga ggtaagagca cgcttcctgt cttgtgcctc
     2581 gaggaatcaa aagggtccgg gcaaggtcac ggtccccgca tcgaacaccc cctgcacccc
     2641 cgggcggggt caccgtgcaa gcccgactcg ggccgcaaag agggcccacc acgtggcgcc
     2701 cacctgctgg aaggccggtg catgtatgcg tgaatccccc ttgtcttgac gccgatggtg
     2761 cgtattatgg agggtgatgc gattcggccc cgctcgacag gcgggggttg ccgtggtgaa
     2821 agggggcgcc tgcatgggga atttcaagga atacgacctc tacgacggga ttggcctcgc
     2881 ggaactcgta aagaaaggcc tggtgtctgc ggccgaggtc ctggaagagg ccatcgagcg
     2941 catagaaagg gtgaacccga agatcaacgc ggtgatcctg cgcatgtacg actcggcccg
     3001 aagggcggtc tcgtccaggc ttcccgaagg tcccttcacc ggggtgccgt tcctcctcaa
     3061 ggacctcctg accgcgtgcg ccggggtgcc cctgagcatg ggctgccgcg cgtacaggga
     3121 ttacgtccct cccgtcgaca gcgagctcat gcgcaggtac agggcctccg gcgtggtggt
     3181 ggcgggcaag accaacaccc ccgagttcgg actcgtggcc gtgacggagc ccgagctctt
     3241 cggccccacc aggaacccat gggatctcgg gcggacgccg ggcgggtcca gcggcgggtc
     3301 ggcagcggcg gtggcgagcg gcatggttcc catggcatcg ggcggcgacg ggggagggtc
     3361 catccgcatc cctgcttccc actgcgggct cttcgggctg aagccgacga ggggccgtac
     3421 gcccacgggg ccgtattacg gcgagatctg gcagggcgcg gcggtggaac acgtgatcac
     3481 caggaccgtc agggacagcg cggccatgct cgacgcgatc cacggggccg accc
//
LOCUS       DAOSCR010000254         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16893_length_3534_cov_2.964932, whole genome shotgun sequence.
ACCESSION   DAOSCR010000254 DAOSCR010000000
VERSION     DAOSCR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16893_length_3534_cov_2.964932"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1090..2304
                     /locus_tag="PLT69_07120"
     CDS             1090..2304
                     /locus_tag="PLT69_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14244.1"
                     /translation="MIIDTDELFGDILATVVDKAFAETRQKYGLLISTLSYGDGDPST
                     DDVEIESVSFSGSGTAARTMRVRFRVLDVDFDAVTLFGYPVVRTRNNDLLVDVSVDLA
                     HTRTGVNDDLVVSVRDVHDVEFEEDYVGDIVVEELVKSDIEDLEPFTFDLDAMLEEIV
                     PPDFIAPCMPSGPQFPDVDTLFSSYLWDVALPGDSTVVLAITQDTFNHVFSKIFVPGF
                     EWDVYKVLRPLFGEGFTGFEESPEENEQTIMSMSVPPLLDMRNDRIRLQLDGVRILYR
                     KDGSDVWEASMDIDLILSARVEGGELAFYLETAPENCHFHIMKDNPGNLGVFDHSNLV
                     NDVVERLPKLLGNPQGGPVFTVSLDDFAPYVEFEQADEPLKVRASGGCLYIEAAASRV
                     DAGWIRDLFTRG"
     gene            2766..>3534
                     /locus_tag="PLT69_07125"
     CDS             2766..>3534
                     /locus_tag="PLT69_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14245.1"
                     /translation="MRRLIRFSIEHPFVVIAAVALVTVAFATQVPKVRMDPRVEIILQ
                     ENNPVERVYAENKKVFEPYADILVGMIHEDVFEPATLAKLDRVAREIEGIKGVKKVQH
                     LLNVKNIQGSNGGLDVSPMVEEGTVPSSPEEAELLRGKVSSWEVYENTYITRDHRGAA
                     IVATLEDGVETDQIVPIYYAIDAIVKKYEGPERFFISGTKVIEALQGHYMIKDLIFLP
                     PLVCVVLLVSLFLFFRHLRGMLLPLVSVAISAVWTVGL"
BASE COUNT          661 a         1052 c         1177 g          644 t
ORIGIN      
        1 gcgagtccat cttcgacccc ggagcgccgg ttcccgcaag gggatttgcg gactcgctcg
       61 acatcgagct cctggccgag acggcgtcgg gaacgtacca ggccatcgag aacggcagct
      121 acgtcgagcc cggcgagagg tcgctcgccg tgcgcataca gggcgcggcc cctgacgtgg
      181 agagggtttt cgtgagtgac gggggcgtgt accaggtcga ggccttgccc ggcgacgacg
      241 ggctgtacag gtgttccttc gaactcaagg cggacaggct atcgaaaccc ctgctcgtcc
      301 aggcgatcca cacggacggg cgggcatcca aggccaaggt cgtggtgagg acctccaccg
      361 agggcaggca ggacgcgctc gtcctgggcg ggatgggcct ggcgataggc gacgagattc
      421 tcgacgcatc ccggggggag gtggagtccc tcctcgcaga ggtgctccgg gaggtaacca
      481 cctgcctcgc ctcgggttcc ggcggggctg tcacggggtt ctccttcgac aggatggtgg
      541 tgaacctcgt ggagtccgcc cctgaagagg tcttcccggg cgccgtcgta cacatcaacc
      601 tgaccctgga gggtgtggac ctctcggcgg tggacgtcta cggggacgac ctcgtggtca
      661 ccacggacaa cgacctcacc gtggacatgt tcgtggcggt cactgacgtg agaacggacg
      721 gcagccgagg gctggtcctc gaactcggga ataccgctac cgcgagtttc cagaaggact
      781 tcttcctcag gccgctcgtg gaggagacgc tctcggcatc gctcggccgc ttcgagctct
      841 cgtgcccttc cgcgacggtc gaggaagccg cttccatgct gaaggaaagg ctccccaaga
      901 cgctcaccct cggggacacg acggtcgact tgagcgagct catctccaac cttgacctgg
      961 atcttgcggg ctatgcgttc ttggacctgt ccggactacc cggtgagggg ggggctcgcc
     1021 tcggcggcat tcgaggcgaa cctctacata ggacaaggct ccctgcagcc aggggctggc
     1081 accgaaccca tgatcatcga cacggacgaa ctcttcgggg atatcctggc gacggtggtg
     1141 gacaaggcct ttgcagagac aaggcagaaa tacgggctcc tcatctcgac cctgtcctac
     1201 ggggacggtg acccctccac cgacgatgtg gagatcgaat cggtctcctt ctcgggctcg
     1261 ggcacggccg caaggaccat gagggtgcgg tttcgggtcc tcgacgtcga tttcgacgcc
     1321 gtcaccctct ttgggtaccc cgtcgtgaga accaggaaca acgacctcct cgtggacgtg
     1381 tccgtggacc tcgcccacac gaggacgggc gtgaacgacg acctcgtcgt ctcggtcagg
     1441 gatgtccatg acgttgagtt cgaggaggac tacgtcgggg atatcgtcgt ggaggaactc
     1501 gtgaagtcgg acatcgagga tctcgagccg ttcaccttcg acctcgacgc gatgctcgag
     1561 gagatcgtgc cgcccgactt cattgcgccg tgcatgccga gcgggccgca gttccccgac
     1621 gtggacaccc tcttctcgag ctacctgtgg gacgtcgccc ttccgggcga ttcgacggtc
     1681 gtgctcgcga tcacgcagga caccttcaac cacgtgttct cgaagatctt cgtccccggg
     1741 ttcgagtggg acgtctacaa ggtgttgagg ccgctcttcg gcgagggctt cacggggttc
     1801 gaagagagcc ccgaggagaa tgagcagacg atcatgagca tgagcgtccc cccgctgttg
     1861 gacatgcgga acgacaggat acggctccag ctcgacggcg tgaggatcct ctaccgcaag
     1921 gacggctcgg acgtctggga ggcctcgatg gacatcgacc tgatcctgag cgccagggtg
     1981 gaagggggcg aactggcgtt ctacctggag acggcgcccg agaactgcca cttccacatc
     2041 atgaaggaca accccgggaa cctcggggtc ttcgaccact cgaacctggt gaacgacgtg
     2101 gtggagaggc tgcccaagct cttggggaac ccccagggag gcccggtctt cacggtgagc
     2161 ctcgacgatt tcgcgccgta cgtggagttc gaacaggcgg acgagcccct gaaggtgagg
     2221 gcctcgggcg ggtgcctgta catcgaggcc gcggcgtcga gggtggacgc aggctggata
     2281 cgtgacttgt tcacgagggg gtgagggaag cgtcccgcga gtcttcggcg ctctgccggc
     2341 ccgcccttga ccggcaaaga ggcggctgcg aggcccccgc gggcgcccga tgaattgttg
     2401 ggggcatggc cgttgacctg tgcccgggcg tcgccggaag ggggcggcgc ggaagaaggc
     2461 ggccacggtg caagggggga cattttcctc ttgcaacaag ggggtttcac aggtatattg
     2521 aaaataatga ggtttcatta ttagagttga atggggcgga tgggtgccgg aagaccgagc
     2581 accgcgatgg cttgagacca gtaaggacat gcatgccaat accatggaag agagtgttgt
     2641 cccgtgattg ataatgagtt ttcattatta aagcattgcg agagcctata cagtgaaggc
     2701 agggggtatg cgatgcactg tgcctgtcgg tgtggaaggt cttcgcctga agacggggtg
     2761 acatcatgag gaggctcatc aggttcagca tcgagcaccc gtttgtcgtg atcgccgccg
     2821 tcgccctggt cacggtggcg ttcgccaccc aggtcccgaa ggtgcggatg gacccgaggg
     2881 tcgagatcat cctccaggag aacaaccctg tcgagcgtgt ctacgccgag aacaagaagg
     2941 tcttcgagcc gtacgcggac atcctcgtcg gcatgatcca cgaagacgtc ttcgagcccg
     3001 cgacgctcgc caagctcgac agggtcgccc gcgagatcga ggggataaag ggcgtcaaga
     3061 aggttcagca cctcctcaac gtcaagaaca tccaggggtc aaacgggggt ctcgacgttt
     3121 cccccatggt cgaagagggc acggtgccgt cgagccctga agaggctgag ctcctgaggg
     3181 gaaaggtctc ctcctgggag gtctacgaga atacctacat tacaagggat caccgggggg
     3241 ccgccatcgt cgccaccctc gaggacggcg tggagacgga ccagatcgtc cccatctact
     3301 acgccatcga cgccatcgtg aagaagtacg aggggcccga gcgtttcttc atatccggga
     3361 ccaaggtgat cgaggcgctc cagggccact acatgatcaa ggacctcatc ttcctgccgc
     3421 ccctggtctg cgtggtcctg ctcgtttcgc tcttcctctt cttcaggcac ttgaggggca
     3481 tgctgctccc cctcgtctcg gtcgccatct ccgccgtgtg gaccgtgggc ctca
//
LOCUS       DAOSCR010000255         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16980_length_3522_cov_2.285838, whole genome shotgun sequence.
ACCESSION   DAOSCR010000255 DAOSCR010000000
VERSION     DAOSCR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16980_length_3522_cov_2.285838"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLT69_07130"
     CDS             complement(<1..320)
                     /locus_tag="PLT69_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14246.1"
                     /translation="MTKRTCILVLALAATALMAVSPLHAAGKGKHSFVPDIPDTWMQV
                     YPEDVDPDFEYNGLRPSCAACPGCDDRFSFFVRGGKTNNLVVYFQGGGACWDTMNCLY
                     VHTY"
     gene            complement(1057..1833)
                     /locus_tag="PLT69_07135"
     CDS             complement(1057..1833)
                     /locus_tag="PLT69_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14247.1"
                     /translation="MSTVITTRRNSSIEILNLGDFLLDGGTMFGRVPKVMWERWFAAD
                     GLNRITMATNVMRFVKDGHTYVVEAGLGRRYPEKSWEIMGTSPERVLTVDEPVDRLVL
                     THLHWDHCGGVHDMEIRDRVYVSRTEWEDAHGETPLSKGSYRAEDLEVIGKRLELVDG
                     PRQIADGVAILPTPGHTRGHISVLIDDEVLYAGDLIPTSAHVHLPCIMAYDLYPLAVL
                     ETKREILSQAVQKGWTVVFEHDPYRPVGKVAFEKGRFTAT"
     gene            2443..3378
                     /locus_tag="PLT69_07140"
     CDS             2443..3378
                     /locus_tag="PLT69_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOJ14248.1"
                     /translation="MNRRVSLFFQTLVIMAAMACLFGLLGWVIFGRGGLVWSVMLALV
                     IFLGTPRISPWVVLRMYRARRLEPVEAQGLYSIVQGLAKRAGLPAAPVLYYVPSGAMN
                     AFSVGTPRASAIALSDGLLRTLSWREIAGVLAHEVSHIKNNDLRIHALADLMTRVTST
                     LSFIGQVLIVFYLPMAVFSDARVPLAPILLLIFAPTVSMLLQLALSRTREFDADLGAA
                     ELTGDPGGLASALRKMESLERRFWPYVFFPARKGLQPSLLRTHPHTEERIRRLDAAAA
                     GKGGSPFVYEGPDLHSPSVYLRLVRPKRRLWPWWG"
BASE COUNT          548 a         1106 c         1188 g          680 t
ORIGIN      
        1 tagtaggtgt gcacgtacag gcagttcatg gtgtcccagc aggcgccgcc gccctggaag
       61 tagacgacga ggttgttggt cttgccgccc ctcacgaaga agctgaacct gtcgtcacag
      121 cccgggcagg ccgcgcagct cggcctgagg ccgttgtact cgaaatcggg gtccacgtcc
      181 tcagggtaca cctgcatcca ggtgtcgggg atgtcgggaa cgaacgagtg cttccccttg
      241 ccggcggcat gcaggggcga taccgccatc agtgccgtcg ccgccagtgc cagtacgaga
      301 atgcatgttc tcttcgtcat agtaccctcc ttgtaccgat gcgagtgcat gtccatcggg
      361 tatgcttcga gccgcgcccc cggcaacgtc ctttgtcgcc gggggcgcat caggcatgcc
      421 ttgatgcggt ccgacacagc acaccacatg gctcccccaa aggtggagac gcacatgctg
      481 tctcacgcgc aaaagcccat aaccggtcct gccttgtgtc ggccgggccc gcgcgtgcct
      541 cttgggtgtt tgcaattgag aagaatatta atggggtacc gaggagagtc aagggtgggt
      601 cgggctcctt gatcggtagg cgctcggtac ccgggttcgg ggtacgaggc cacgtcgtct
      661 tctcggcata ggtgagacct ggatgccgag aaacaggaga cctgtgcgcc ccgggtccac
      721 gttgcccttc ctcgggactc gggggtaagt ccggggctga ccgtgggctt cggcgtggtg
      781 atcacccatg gagccggctt ggatccgatg agccccgggt gtggaggtgg tatggtatgc
      841 ggtcctgcct acgccaagca gtgatcgaca cgatgcgtct cggaaagagg ggccggcgcg
      901 tgtgccggct gaatgcatcc gcccaggtct tcagcctgcc gggaggccca tggaacatgc
      961 accccaggtg gtctgaggtg acgtggtgtg gcctacggtt tgtcccctgt cttcttggtg
     1021 aatgaacaca gggaaagaga accttcgccg tggtgttcag gtggccgtga acctgccttt
     1081 ctcgaaggcg accttgccca cggggcggta cgggtcgtgc tcgaacacga ccgtccagcc
     1141 tttctgtacc gcctgcgaga ggatttccct cttggtctcg aggacggcga gcgggtacag
     1201 gtcgtaggcc atgatgcacg gcaggtggac gtgggcggag gtggggatga ggtcgcctgc
     1261 gtagaggacc tcgtcgtcta tgagcacgct tatgtgcccc ctcgtgtgcc cgggggtggg
     1321 caggatcgcg acgccgtctg ctatctgcct gggcccgtcc accagctcga gtctcttccc
     1381 aatgacctcg aggtcctcag cccggtagct ccccttgctc agcggggtct ctccgtgggc
     1441 gtcctcccac tcggtgcgcg atacgtacac cctgtctcgt atctccatgt cgtgaacgcc
     1501 gccgcagtgg tcccagtgga ggtgggtgag caccaggcgg tccactggtt catcgacggt
     1561 gagcacccgc tcggggctcg tgcccatgat ttcccagctc ttttcggggt agcggcggcc
     1621 gagccctgcc tcgacgacgt aggtatgacc gtccttgacg aacctcatca cgttcgtggc
     1681 catggtgatg cggttgagtc cgtcggctgc gaaccacctc tcccacatga ccttgggtac
     1741 cctgccgaac atggtcccgc cgtcgaggag gaagtcgccg aggttcagga tctcgatgga
     1801 ggagttcctg cgggttgtga tcaccgttga catgtccaga cctgtatcat cgggcgtcac
     1861 ggctgtaaac cccgttcggc ggtcccgatg gccgtgtcgc gcggtatccc ccctcacgac
     1921 acaccgtacg ccatgtcctt tccttattga ctcgggtctc aggcccggtt cgcatcccgg
     1981 ctcctgccac gacgagtcgt gtccttatgg ctctggtttc aggggggaga ggaggagatt
     2041 gccctctcac cggccgtcat gtcccagtaa gatcgggttt cggttgcgtg aagggtggcc
     2101 gggggaatcg ggagggggct tgaggtactg cacggaagcg ggagggaaaa ggagtttgaa
     2161 agtcgcgatg tcctgcacta tggttggtga aggcggaccg ggaacgggca agcgccaggt
     2221 ggaccctcta ggaaaggcgc tgcgccggcc gcggcgccgg gcgcgctcgg ggaacccggg
     2281 catccacctt gtgcgaggcc tccatggcca tgcaggcaga agggggagag acgccggcag
     2341 cggtctctga cctcgcctgc cgcccaggcg cctggggttg gcggcgcgtc agccgcgcaa
     2401 gcggtatccg gaggccgccc tcgaaaggag atgcagacgg tcatgaaccg gcgggtatcc
     2461 ctctttttcc agaccctcgt catcatggcg gccatggcct gcctgttcgg cctgctcggg
     2521 tgggtgatct tcggcagggg cggcctggtg tggtcggtca tgctcgccct cgtcatcttc
     2581 ctgggcacgc cccggatctc gccgtgggtc gtgctccgca tgtacagggc acgcaggctc
     2641 gagcccgtgg aggcccaggg gctctactcg atcgtccagg gcctcgcgaa gagggcgggg
     2701 ctgcctgccg cgcccgtgtt gtactacgtg ccgagcggcg cgatgaacgc cttcagcgtg
     2761 ggcacgccca gggcctcggc aattgccctc tcggacgggc tcttgaggac cctctcgtgg
     2821 agggagatcg ccggggtgct cgctcacgag gtctcccaca tcaagaacaa cgacctcagg
     2881 atccacgcgc tcgccgacct catgacgagg gtgacctcga ccttgtcgtt catcgggcag
     2941 gtcctcatcg tgttctacct gccgatggcc gtcttctccg acgcgagggt gcccctggcc
     3001 cccattctcc tgctcatctt cgcgcccacg gtgagcatgc tcctccagct cgcgctctcg
     3061 cgaacccgcg agttcgacgc cgaccttggc gccgcggagc tgacagggga cccaggaggg
     3121 ctcgcaagcg cgctcaggaa gatggaaagc ctggaacgga gattctggcc gtacgtcttc
     3181 ttcccggcga gaaagggcct ccagccgtcg ctgctccgca cccacccgca taccgaggaa
     3241 cgcatcaggc ggctcgatgc cgccgccgca ggaaaggggg gctccccctt cgtgtacgag
     3301 gggcccgatc tgcacagccc gagcgtatac ctccggctcg tgaggccgaa gaggcggctg
     3361 tggccgtggt gggggtgagt cgtgcaccga gcggcctccc tgcggggcgc caagggcgct
     3421 cacgcaggct ctctctactt tcccttcggc atgtggccgc ctgcgaattc ccggcctggc
     3481 gccaagggcg ctcgtgaaga ctcttcacgt ggcgggtgcg gg
//
LOCUS       DAOSCR010000256         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_16989_length_3520_cov_3.312843, whole genome shotgun sequence.
ACCESSION   DAOSCR010000256 DAOSCR010000000
VERSION     DAOSCR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16989_length_3520_cov_3.312843"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PLT69_07145"
     CDS             <1..1393
                     /locus_tag="PLT69_07145"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOJ14249.1"
                     /translation="VIRNVAPTVDAGPDRTVSAGSRVTLHATGADPNEDSLSYTWTKV
                     NGPSVTLPSLSQQDITFTPTIAGQYTFSVRCSDGVNTSSADQVVVTVNATNSAPTANA
                     GVDQDVSVGTVVRLDGSGSTDPDGDALSYSWSQVSGPSVALSSATAQSPTFTAASVGT
                     YVFQLVVSDGKVSSTADQVTVRAVSANTPPVADAGDDIRALVGDDVILDASRSYDPDT
                     DGLTFAWSQVSGATVQIHNATTMRAFFTPTTSGVYTFKVSVSDGQATSTDTVSVTVDN
                     ANQVPVAVAGDAIVAVVGQTVTLDGSASYDPDGDAISYVWSQAGGPSVTLSASNTARP
                     SFVAAQAGVYVFELRVYDGEDTSSASNVTVTVQNAAGQITLVSPTNGAVCYSSPTLEW
                     AGEGFKSYKAYISIDGKRYSTVYSGTGTSTTLHSVLWRWFIPAGTTVRWYVVGTTFDG
                     RTVKSDTQTFIRR"
     gene            complement(1528..2649)
                     /gene="alr"
                     /locus_tag="PLT69_07150"
     CDS             complement(1528..2649)
                     /gene="alr"
                     /locus_tag="PLT69_07150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ14250.1"
                     /translation="MRPTRVEIDLSAIRHNLTLIKQFTKNRVLMAIKADAYGHGAETV
                     GRFVAENALADMLGVSCIEEGLQLRAAGVRLPILVFGLLTGSPDEIEAVFSEDLIPTV
                     VDLQPVDALVKGARRWNRKVSVHVKTDTGMGRLGLPPEKTFAVLMRLSSVPEITIGGL
                     YSHFPVSDEPSNPFTSEQIARFIDLSNRLAGAGIPCGLKHLANSGGIINHPESHLDMV
                     RPGILCYGLYPSEETPRILDVIPAMTLKTAVMFTKRVKKGTGLSYGLTYRPEKDTNIA
                     TLPIGYADGYPRSLSNNAFVLIDGKQYPVVGRVCMDQTLVDLGDDTYPQGHEVVLFGN
                     TSITAATLSSWRGTIPYEVICDMSRRVPRMYSGTTEAAA"
     gene            complement(2659..>3520)
                     /locus_tag="PLT69_07155"
     CDS             complement(2659..>3520)
                     /locus_tag="PLT69_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ14251.1"
                     /translation="MGGGTGTGASPVVARVAREMGVLTVAVVIKPFSFEGKLKMTKAE
                     AGEKELAASVDAIIAIPNDRLLSTAGDQTMMNAFKTADNILLDAVRGISEVMSNTGLV
                     NVDFNDVKNIMKEKGKALMGIGRATGPNRAAEAAQSAISSPLLEDMDIHGAKGILINI
                     TADQSLTMNEVHEASSIIRAAAHEEADIKWGVVIDEAAGEEMSITVIATGFASKPVVL
                     SSGIEKGKVHQTQRDAQSATVVDYSKPAYMRKKEEKEKEVIRLGMVVDDSVLDQELAK
                     FPPFLRKKAD"
BASE COUNT          547 a         1170 c         1161 g          642 t
ORIGIN      
        1 ggtcatcagg aacgtggcgc ccaccgtcga cgcaggcccc gacaggaccg tcagcgcggg
       61 ctccagggtt acgctgcacg cgaccggggc ggaccccaac gaggacagcc tcagctacac
      121 gtggaccaag gtgaacgggc cgagcgtgac cctgcccagc ctctcgcagc aggacatcac
      181 cttcaccccg accatcgcgg gccagtatac cttctctgtc cgctgctcgg acggcgtcaa
      241 caccagttcc gccgaccagg tcgtggtgac ggtgaacgcg accaacagcg cgcccacggc
      301 gaacgccggc gtcgaccagg acgtgagcgt gggcaccgtg gtgaggctcg acggctcggg
      361 ttccaccgac ccggacgggg atgccttgag ctattcctgg agccaggtct ccgggccctc
      421 ggtcgcgctc tcgagcgcaa cggcccagag cccgaccttc acggcggcct ccgtgggcac
      481 ctacgtgttc cagctcgtgg tctctgacgg caaggtgtcc tccacggccg accaggtcac
      541 cgttcgtgcc gtctcggcca acacgccgcc cgtcgccgac gcgggtgacg acatccgcgc
      601 cctcgtgggc gacgacgtga tcctcgacgc ctcgaggtcg tacgaccccg acaccgacgg
      661 gctgaccttc gcgtggtccc aggtctcggg cgcgaccgtc cagatccaca acgcgaccac
      721 catgagggcg ttcttcacgc ccaccacctc gggcgtgtac acgttcaagg tgagcgtgag
      781 cgacggccag gcgacatcca ccgacaccgt gagcgtgacc gttgacaacg cgaaccaggt
      841 cccggtggcc gttgcagggg atgcgatcgt cgcggtggtg ggccagacgg tgacgctcga
      901 cggcagcgcc agctacgatc ccgacgggga cgccatctcg tacgtgtggt cgcaggcggg
      961 cgggccttcc gtgacgctct ccgcttccaa cacggccagg cccagtttcg tggccgcgca
     1021 ggcgggcgtc tacgtcttcg agctgagggt gtacgacggc gaagacacga gcagcgcctc
     1081 gaacgtgacc gtgacggtgc agaacgcggc cggccagatc acgctcgtga gcccgacgaa
     1141 cggggccgtg tgctactcga gcccgaccct cgagtgggcc ggggaaggct tcaagagtta
     1201 caaggcgtac atatccatag acggaaagcg ctacagcacc gtctacagcg ggaccggcac
     1261 ctcgaccacc ctccactcgg tgctctggag gtggttcatc ccggccggca cgacggtgag
     1321 gtggtacgtg gtgggcacca ccttcgacgg ccgcacggtg aagagcgata cccagacgtt
     1381 catcaggcgt tgacggccgg gaggcgagcc cggcgtgaca ggggcggctt cggccgcccc
     1441 ttctcttttg ggaataccgg cttgtggtgg gcgtccttac caccccgggc gcgcccgtga
     1501 acccgggcac ggaaggcgag cccggggtca ggccgccgct tcggtggttc ccgagtacat
     1561 cctcggcacc cgcctgctca tgtcgcagat cacctcgtag gggatggtgc ccctccacga
     1621 cgagagggtg gcggcggtga tggaggtgtt cccgaacagc acgacctcgt ggccctgggg
     1681 gtaggtgtcg tcaccgaggt cgacgagcgt ctggtccatg cagaccctcc cgaccacggg
     1741 gtactgcttg ccgtcgatga ggacgaaggc gttgttggag agcgagcggg ggtacccgtc
     1801 ggcataccca atggggagcg tggcgatgtt tgtgtctttc tccgggcggt aggtgagccc
     1861 gtaggacagg cccgtcccct tcttcacgcg cttggtgaac atcaccgcgg tcttgagcgt
     1921 catggccggg atcacgtcga ggatcctcgg cgtctcctcg gaggggtaca ggccgtaaca
     1981 gaggataccg ggcctgacca tgtcgaggtg cgactccgga tggttgatga tcccgcccga
     2041 gttggccagg tgcttcagcc cgcaggggat gcccgcgccg gccagccggt tcgagaggtc
     2101 tatgaacctg gctatctgtt ccgacgtgaa ggggttggac ggttcgtcgg agacgggaaa
     2161 gtgggagtac aggccgccga tggtgatctc gggaacggag gagagcctca tgagcaccgc
     2221 gaatgtcttc tctggaggca acccgagcct tcccatgccc gtgtccgtct tcacgtggac
     2281 cgagaccttc ctgttccatc tcctcgcgcc cttgacgagc gcgtccaccg gctggaggtc
     2341 cacgacggtg ggtatgaggt cttccgagaa gacggcctcg atctcgtcgg ggctgcccgt
     2401 gaggagcccg aacacgagga tgggcaggcg cacgcccgcg gccctgagct ggaggccctc
     2461 ctctatgcag ctcacgccga gcatgtccgc cagggcgttc tcggcgacga acctcccgac
     2521 cgtctccgcg ccgtggccgt aggcgtcggc cttgatggcc atcaggacgc ggttcttggt
     2581 gaactgcttg atgagggtca ggttgtgtct tatggccgag aggtctatct ctacacgcgt
     2641 cggcctcatg ggcctccctc agtccgcctt cttgcgtaag aacgggggga acttcgccag
     2701 ttcctggtcg aggacgctgt cgtccaccac catgccgagc ctgatgacct ccttttcctt
     2761 ctcctccttc ttgcgcatgt acgccggctt gctgtagtcg acgacggtgg cgctctgtgc
     2821 gtccctctgg gtctggtgga ccttcccctt ctcgatcccc gaggacaaga ccacgggctt
     2881 gctcgcgaac cctgtcgcga tcacggtgat ggacatctcc tctccggccg cctcgtcgat
     2941 gacgaccccc cactttatgt ccgcttcctc gtgcgcggcc gcccgtatga tggaggacgc
     3001 ctcgtgcacc tcgttcatgg tcaggctctg gtctgccgtg atgttgatga ggatgccctt
     3061 tgcgccgtgg atgtccatgt cctcgagcag gggggaactg atggccgact gcgcggcctc
     3121 ggcggcgcgg ttggggcccg tcgccctgcc tatgcccatg agcgccttgc ccttctcctt
     3181 catgatgttc ttcacgtcgt tgaagtcgac gttgacgagc ccggtgttgc tcatcacctc
     3241 ggagatgccc ctgaccgcgt ccaggaggat gttgtcggcc gtcttgaacg cgttcatcat
     3301 ggtttggtca ccggccgtgg agaggagcct gtcgttgggt atcgcgatga tggcgtcgac
     3361 gctggcggcc agctccttct cccccgcctc ggccttggtc atcttgagct tgccctcgaa
     3421 ggagaacggc ttgatgacca ccgccaccgt gagcacgccc atctccctgg ccaccctggc
     3481 gaccacgggc gaggcgccgg tgcctgtccc gccgcccatg
//
LOCUS       DAOSCR010000257         3509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17057_length_3509_cov_3.848292, whole genome shotgun sequence.
ACCESSION   DAOSCR010000257 DAOSCR010000000
VERSION     DAOSCR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17057_length_3509_cov_3.848292"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..1080
                     /locus_tag="PLT69_07160"
     CDS             544..1080
                     /locus_tag="PLT69_07160"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HOJ14252.1"
                     /translation="MNPAAGEGFTLAELTAVVVVVCVLAGLALPVLGPFFMRSAITRQ
                     ASEIHQKIVHARDLAMRQRVCWRVCFVPGESRWYCHADTDGNGRRDAGEPFVGPYVLQ
                     RGIRFGSRAARGPNDTEIPRDGVSLSDNRVSFSAMGTCNSGTVYLTSGSRDMALRVMP
                     ASGVVLEYEWDDGWRAAR"
     gene            1077..1499
                     /locus_tag="PLT69_07165"
     CDS             1077..1499
                     /locus_tag="PLT69_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ14253.1"
                     /translation="MKSGFGIIEVMIALAMLMVCALAAFRTIQVCMHAAHYSETMSRA
                     AVLCRTQAASLWAEPQASPALSPGWHKDPANPIIEGGMTFYRFWSVQEAELGLRTRVY
                     VAWSEKGRAASCEFDSEEEAEASVQPKVTMSGLIPSWP"
     gene            complement(1626..3260)
                     /gene="groL"
                     /locus_tag="PLT69_07170"
     CDS             complement(1626..3260)
                     /gene="groL"
                     /locus_tag="PLT69_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415963.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ14254.1"
                     /translation="MAGKQILYNQEARSKVLAGVNKLADAVKATLGPKGRNAILDKMF
                     GAPNVTKDGVTVAKEIELKDKFENMGAQMVREVASKTSDVAGDGTTTATVLAQAIYTE
                     GLKYLAAGMNAMDLKRGIDKATAAVVEELKKMSRKVEDKKEIEQVGTISANGDNEVGA
                     LIADAMDKVGKEGVITVEEAKGMETTLELVEGMQFDRGYLSPYFVTDAERMEAVLEDP
                     YILIFDKKISNMRDIIPILEQIAKEGRALLIIAEDVEGEALATLVVNKLRGTLKCAAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEELGRKLESASLADMGTAKRIIIDKDNTTIVE
                     GAGKKAAIQGRITQIKAQIEETTSDYDREKLQERLAKLAGGVAVIKVGAATEPEMKEK
                     KARVEDALHATRAAVEEGFLPGGGVALLRCIDKLNSLDLEGDQKFGVKIVQKALEEPL
                     RQIAVNAGLEGGIVVEKVKSLKGNHGFNAATEEYEDLLKAGIIDPTKVTRFALQNAAS
                     VAGLLLTTEVMVAEIPEEKKTGPGAGGMPHGMDDMM"
     gene            complement(3276..>3509)
                     /locus_tag="PLT69_07175"
     CDS             complement(3276..>3509)
                     /locus_tag="PLT69_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ14255.1"
                     /translation="QKTKGGIIIPDTAKEKPQMGEVIAAGPGRKNEEGKVFPLDVKKG
                     DKVLFSKYAGNEIKIEGVEYLIMREEDILGIVK"
BASE COUNT          517 a         1180 c         1095 g          717 t
ORIGIN      
        1 cgggggctcg gggtcaccgc ggccatgctc cgggacatgg gcatcgccgt cgtgaacgag
       61 gatgcggacg agtgatttct gggccggcat gggcacggtg tgaaggcggg tgaggacacg
      121 catcgcgagg ggccggccgc ggcgtttcgc cggtgccgca tgagcgccga gcgggtgaag
      181 acccgtgatg ccgagtgtcg tgtgaaggat atggcctcct ctcgaggtca ggacaaggcg
      241 ctgcggccag agcgggaaga cctgcgcgtc aaaaggcaag gccgagaatc cccctttgac
      301 catggacctc cgtgctcgac tgccgatcat gggtgtcatc ccgcagcctt cctgcgtggc
      361 gcgcgcaccg aggtgtgccc ttttcttggc cggggatgcg tactataatc gaacatcgag
      421 aaggctcgaa ccgagcgtcg tacccttgaa gccctattcg gtgagcgtgc ctttctcttt
      481 tcatggaata tgcgctgcgc atgcagtcat ggtgtacgtg gagaggtcgg aacaggccgt
      541 ctggtgaacc ccgcggccgg agaggggttc accctcgccg agctgacggc cgtcgttgtc
      601 gtcgtgtgcg tgctggctgg tctcgccctg ccggtgctcg ggcccttctt catgcgctcg
      661 gccatcacga gacaggcgag cgagatccac cagaagatcg tccacgcgag agacctcgcc
      721 atgaggcagc gggtctgctg gagagtctgc ttcgtccctg gtgagtcgag atggtactgc
      781 catgccgata ccgacgggaa cggccgccgc gacgccggcg aacccttcgt gggcccctat
      841 gtcctccaga ggggtattcg gttcggttcg agggccgcca ggggcccgaa cgacacggag
      901 atcccccgcg acggggtgag cttgtcggac aaccgcgtga gtttctcggc catgggcacg
      961 tgcaactcgg gcaccgtgta cctcacgtca gggagcaggg acatggcctt gagggtcatg
     1021 cccgcttccg gcgtcgtcct cgagtacgaa tgggacgacg gctggagggc cgccaggtga
     1081 agagcgggtt cgggatcata gaggtgatga tcgctctcgc catgctcatg gtgtgcgcgc
     1141 tggcggcgtt ccgcacgatc caggtctgca tgcacgccgc ccattacagc gaaaccatga
     1201 gcagggcggc agtcctgtgc cgcacccagg cggcttcctt gtgggctgag ccgcaggcat
     1261 cccccgccct gtcgccgggc tggcacaagg acccggccaa cccaatcatc gagggcggga
     1321 tgacgttcta ccgtttctgg tccgtccagg aggcagagct cggcctgaga acccgtgtgt
     1381 acgtggcctg gtcggaaaag ggcagggctg cctcctgcga gttcgactcg gaggaggagg
     1441 cggaggcctc tgtgcaaccc aaggtgacca tgagcgggtt gatcccttca tggccgtgaa
     1501 gtgaagggtg gccctgcaag acggtcgata ccggcggggt cctctccccg gtcgggagcc
     1561 gggcataaaa acacggcggc gtgtcgaaca cgccgccgtg ttcagcctcg atgtcaaggg
     1621 cccgtttaca tcatgtcgtc catgccgtgg ggcatgccgc ctgccccggg gccggtcttc
     1681 ttctcctccg ggatctctgc gaccatgacc tcggtggtga gcaagagccc ggccacggac
     1741 gccgcgttct ggagcgcgaa gcgcgtcacc ttggtggggt cgatgatacc ggccttcaag
     1801 aggtcttcgt actcttcggt cgcggcgttg aagccgtgat tgcccttcag ggacttcacc
     1861 ttctcgacca cgatcccgcc ctcgaggccg gcgttcaccg cgatctgcct gagaggctcc
     1921 tcgagcgcct tctgcacgat cttcaccccg aacttctggt cgccctcgag atccaggctg
     1981 ttgagcttgt cgatgcagcg cagcagcgcc acgccgccgc cgggcaggaa cccttcctcg
     2041 acggccgccc tagtggcgtg cagggcgtcc tcgaccctcg ccttcttctc cttcatctcg
     2101 ggctcggtcg cagcgcccac cttgatcacc gccacgccgc cagcgagctt cgccagccgc
     2161 tcctgcagct tctcacggtc gtagtccgag gtcgtctcct cgatctgcgc cttgatctgg
     2221 gtgatgcggc cctggatggc ggccttcttg cccgcgcctt ccacgatcgt cgtgttgtcc
     2281 ttgtcgataa tgatccgctt ggccgtcccc atatcggcca gagacgcgct ctcgagcttg
     2341 cggcccagct cctcggagat cacctggccg ccggtcagga tggcgatgtc ctcgagcatg
     2401 gccttgcggc ggtcaccgaa cccgggcgcc ttcacggccg cgcacttgag cgtgcccctg
     2461 agcttgttca ccaccagcgt cgcgagcgcc tcgccctcga cgtcctcggc gatgatgagg
     2521 agagcccggc cctccttggc gatctgctcg aggatcggga tgatgtcacg catgttgctg
     2581 atcttcttgt cgaagatgag gatgtagggg tcttcgagca cggcctccat gcgctcggcg
     2641 tcggtcacga agtaggggga gaggtaaccg cggtcgaact gcatgccctc cacgagctcg
     2701 agggtggtct ccatgccctt ggcctcctcg accgtgatca cgccctcctt gcccaccttg
     2761 tccatggcgt ccgcgatcag ggccccgacc tcgttgtcgc cgttggccga gatcgtcccc
     2821 acctgctcga tctccttctt gtcctccacc ttgcggctca tcttcttcag ctcctccacc
     2881 accgcggccg tcgccttgtc gatcccgcgc ttgagatcca tggcgttcat gcccgccgca
     2941 aggtacttca gcccctcggt gtagatcgcc tgcgccagca ccgtggccgt cgtcgtcccg
     3001 tccccggcca cgtcggaggt cttgctcgcc acctcccgca ccatctgggc gcccatgttc
     3061 tcgaacttgt ccttcagctc gatctccttg gccaccgtca ccccgtcctt ggtcacgttc
     3121 ggcgccccga acatcttgtc caggatcgcg ttgcgcccct tcggcccgag tgtcgccttc
     3181 accgcgtccg caagcttgtt cacgcccgca aggaccttgc tccttgcctc ctggttgtaa
     3241 agaatctgct ttccagccat cgcgtacctc cgctctcact tgacgatgcc gaggatgtcc
     3301 tcttccctca tgatgaggta ctcgacgccc tcgatcttga tctcgttgcc cgcgtacttg
     3361 gaaaagagca ccttgtcgcc cttcttcacg tccagcggga acaccttgcc ttcctcgttc
     3421 ttcctgcccg ggcccgcggc gatcacctcg cccatctggg gcttctcctt ggcggtgtca
     3481 gggatgatga tgccgccctt ggtcttctg
//
LOCUS       DAOSCR010000258         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17074_length_3507_cov_3.331692, whole genome shotgun sequence.
ACCESSION   DAOSCR010000258 DAOSCR010000000
VERSION     DAOSCR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17074_length_3507_cov_3.331692"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            705..2549
                     /locus_tag="PLT69_07180"
     CDS             705..2549
                     /locus_tag="PLT69_07180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ14256.1"
                     /translation="MNWKEKLVSPDEALQQLEPGMKILLATGSAEPRTLIRHLLASDA
                     PNLQDLELIQIVSLGDLIRVKDLPAHKFRLKTFFSGWVASEAITGGHVDLVPSRFTRI
                     PSLIETGLLPVDAAFIQVTPPTETGYVSLGVSVDVARQAIEQARLVVGEINDKVPFTY
                     GDTFLHVDEFDAFISSEDDPLYLERWPVDEVFDQVAQNIASVIEDGSCLLFSIGPIYE
                     ALSRHLVRKRNLGIHSPFFTDAVMDLVKSGAVTNRYKGVFKGKSLASYAFGTKELFSW
                     LDRNPLIEFQGIDLVFNPVEIGRNPRFIAIIPARKVDISGRIAMHIGKGAVATTPGEA
                     MDFINGAELSRGGCTVFALPSRNRKGRPNILVSVEELPNLLGLRESVDMVVTEYGVAN
                     LKGRTVRERAQALIDIAHPEDRQALVEEAKRANILYQDQIYIPSCEYLFPSQISDKRT
                     FKGGVEVRFRHIRPSDEEEMRRLFYRFSDKTVYYRYFSPIKTMPHAKMQQYVNVDCNR
                     ACSIVALVGPPEAERIIAEARFVKDRSRPYADVAFVVDEQYQGLGIASHMLSMLTRVA
                     KERGLQGFMADVLASHKAMLKVFERSGLPIRATLASGVYEMTMPFYEE"
     gene            complement(2756..>3507)
                     /locus_tag="PLT69_07185"
     CDS             complement(2756..>3507)
                     /locus_tag="PLT69_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ14257.1"
                     /translation="SASVDIDRQRARGICGAMMFEGDDALKKIEVLSGGEKSRVMLGK
                     ILATPVNVLLLDEPSNHLDMESTDALLAALDSFEGAVVMVTHNELLLDAIADRLVVFR
                     NGALEVFEGGYDWFLRKGGWGDDEEAAACSRREARQHPDGGKPSKKELRRRRGEIVAE
                     RSRVIGPIEKRMAFVESEIERLERRLACHNEELQSASFARDGAKVVEVSRLMHECEQA
                     IEAFFGELEALHGELERHRAAFEAQLAGLED"
BASE COUNT          618 a         1196 c         1079 g          614 t
ORIGIN      
        1 gaccgtctcg aggctcgact ccgccaccga gatcgcggtc accatgacga acgagggtct
       61 caggacaggg ctggcggtcg tgttcgtgct catcgcgctt gtcttcgtgt accgctcctt
      121 ctacgctatg cggatcacgg gcgtgaagta agggcataca cagaggcgaa cgttcctcga
      181 ggggcgcccc cgaaaagggg cgcccctcga ttttcctgag tcctggccgg caggccgcac
      241 gaagacccaa ggggatgagc ctcgacggcc gcggccataa agacgccctg acacgacgtc
      301 gatacaggcg ccgcgaagag ggcctggagg tcctcttcat gtgcggggcc ccttccttct
      361 ggtgtacctt cagctgctcg tggcacatga gaacgcgagg cgtctccgca cccggacggc
      421 atggcggtgg ccgggcggga cgggcgggtc acgtgggcgt cccaccgagg tcgcgtgtcc
      481 gggtcgtctg gaaaacctcg ttctccggtg attgcgatga acccgcgttg tgaggtatcc
      541 tgggcgtcta ggtgctcgat ccctgaaggc gcacgggagg gcctgggacc gccgcatgac
      601 gggagacccg gccgtcaggt gatcgcgatc gggtgcacga ggagggcatc gtctctccga
      661 gaggctgtct ttcaccgtca cgatacaggg ggatgaacgt tcacatgaac tggaaagaga
      721 agctggtaag cccggacgag gcgctccaac aactcgagcc gggcatgaag atcctcctcg
      781 ccacgggttc cgcggagccg cgcaccctca tccgccatct cctggcgagc gacgcgccga
      841 acctccagga cctcgagctc atccagatcg tgagcctcgg cgacctcatc agggtcaagg
      901 atctccctgc ccacaagttc aggctcaaga cctttttctc cgggtgggtc gccagcgagg
      961 ccatcaccgg cggccatgtg gacctcgtac cgagcaggtt cacgaggatc ccctccctca
     1021 tcgagacggg cctgttgccc gtggacgcag ccttcatcca ggtgacgccg ccgacagaga
     1081 ccgggtacgt gagcctcggc gtgtccgtgg acgtggccag gcaggccatc gaacaggcga
     1141 gactcgtggt cggcgagatc aacgacaagg tcccgttcac ctacggggac acgttcctcc
     1201 acgtggacga gttcgacgcc ttcatctcct cggaggatga cccgctgtac ctcgagaggt
     1261 ggcctgtcga cgaggtcttc gaccaggtgg cgcagaacat cgcatcggtc atcgaggacg
     1321 ggagctgcct gctcttctcg atcggcccca tctacgaggc cctctccagg cacctcgtga
     1381 ggaagcgcaa cctcggcatc cactccccgt tcttcaccga cgcggttatg gatctcgtga
     1441 agagcggggc cgtcaccaac aggtacaagg gcgtgttcaa gggaaagtct ctcgcctcgt
     1501 acgcgttcgg cacgaaggag ctcttctcgt ggctcgacag gaaccccctc atcgagttcc
     1561 aggggatcga tctcgtgttc aaccccgtcg agataggccg caacccccgg ttcatcgcga
     1621 tcatccccgc ccgcaaggtg gacatatcgg gacgcatagc catgcacatc gggaaggggg
     1681 cggttgcaac cacgcccgga gaggccatgg acttcatcaa cggcgccgag ctctcacggg
     1741 gtgggtgcac cgtcttcgct ctccccagcc gcaaccgcaa gggccggccc aacatcctcg
     1801 tgtccgtgga agaactcccg aaccttctgg gcctgaggga atctgttgac atggtggtga
     1861 cggagtacgg cgtcgccaac ctcaagggcc gcaccgtgag ggaacgggcg caggcgctca
     1921 tcgacatcgc ccaccccgaa gaccgccagg cgctcgtcga ggaggcgaag cgggcgaaca
     1981 tcctctatca ggaccagatc tacatcccct cctgcgaata cctcttcccg tcccagatca
     2041 gcgacaagag gaccttcaag gggggcgtcg aggtgcgctt ccgccacatc aggccctcgg
     2101 acgaggagga gatgaggagg ctcttctacc ggttctcgga caagaccgtg tattaccgct
     2161 acttcagccc cataaagacc atgccccacg ccaagatgca gcagtacgtg aacgtcgact
     2221 gcaaccgcgc ctgttccatc gtcgcgctcg tcggtccgcc cgaggcagag cgcatcatcg
     2281 ccgaggcgcg tttcgtcaag gacaggagca ggccgtacgc cgacgtggct ttcgtggtcg
     2341 acgagcagta ccaggggctg ggcatcgcgt cgcacatgct ctcgatgctc acgagggtag
     2401 ccaaggagag gggcctccag ggattcatgg ctgacgtgct cgcatcccac aaggccatgc
     2461 tcaaggtctt cgagcgctcg ggcctgccca tccgggcgac gctggccagc ggtgtctacg
     2521 agatgaccat gccgttctac gaagagtgac cgcaggctcg ggcgtctgga tcctccggcc
     2581 ctggcgaggc cgcacccgcc tcccccggat cgaggagcac gaggggccga cgccctcttc
     2641 cccggtacgg ccgccgcctg ggtgcatcgc gcgaccatcc cccaaggaca ccgtgtcgcc
     2701 gtcgtgggtg cactcccctc ttgtcgggcc ctttccaggc cgggacatcc ggtcgtcaat
     2761 cctcgagccc tgcgagctgc gcctcgaagg ctgccctgtg acgctcgagt tcaccgtgga
     2821 gcgcctcgag ctctccgaag aaggcctcga tcgcctgttc gcactcgtgc atcagcctcg
     2881 agacctccac gaccttcgcc ccgtctcgcg cgaagctcgc actctggagc tcctcgttgt
     2941 ggcatgcgag ccgccgctcg agcctctcga tctcggactc gacgaacgcc atccgtttct
     3001 cgatggggcc gatgacgcgc gaccgctccg caacgatctc ccccctccgc ctgcggagtt
     3061 ccttcttgct cggcttccct ccatcggggt gctgccgcgc ttccctgcgg gagcaggccg
     3121 ccgcctcctc gtcgtcaccc cagcctccct tcctgaggaa ccagtcgtag cccccctcga
     3181 acacttccaa tgcgccgttt ctgaacacga cgagcctgtc ggcgatcgca tcgagcagca
     3241 gctcgttatg ggtgaccatc acgacggcgc cttcgaacga gtccagggcg gcaagcaggg
     3301 cgtcggtgga ctccatgtcg aggtggttcg acggctcgtc cagaaggagc acgttcaccg
     3361 gcgtcgccag gatcttgccg agcatgaccc tgctcttctc cccgcccgag aggacctcga
     3421 tcttcttgag cgcatcgtcc ccctcgaaca tcatggcgcc gcatatgccc cgggctctct
     3481 gccgatcgat gtccacagag gcgctca
//
LOCUS       DAOSCR010000259         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17315_length_3468_cov_4.106065, whole genome shotgun sequence.
ACCESSION   DAOSCR010000259 DAOSCR010000000
VERSION     DAOSCR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17315_length_3468_cov_4.106065"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLT69_07190"
     CDS             <1..983
                     /locus_tag="PLT69_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOJ14258.1"
                     /translation="FMKGLPNRPPVLLKKGEGIAGKVAQTAKTIYIPDVTKDPNFCDK
                     GTRQEGSLACVPIISEGECIGVINFRDNAPGAFGPNDLRFFELIADQLSITASLVRTY
                     QDLLDLEKKRMNLSRYFSKGLAEHLLTDSRMTRLGGEKKAVTVLFADIVGFTSLVEKN
                     PVEKVVVVLNLFFETVVPIIFSHGGMLDKFLGDGLMAVFGIPDADDLDPVRAVRCATD
                     IQRAMAGLRDRFEREGYFSIEVGIGISSGVVVAGNIGTLEHMNYTVIGETVNLAQRLE
                     SISDPGQIIVSDVTRRLIPDHAPIDVSFEPMGTMRLKGIDREIAPFRVVY"
     gene            1276..2523
                     /gene="rho"
                     /locus_tag="PLT69_07195"
     CDS             1276..2523
                     /gene="rho"
                     /locus_tag="PLT69_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOJ14259.1"
                     /translation="MHLEDLKKMDPKELLETANALEIEGSATMNKQELIFAILQAQAE
                     KDVSISGKGVLEILPDGFGFLRSADYSYLPGPDDIYVSPSQIRRFNLKNGDEVNGFIR
                     PPKDNERYFALLKLESINMEDPEAAKHRVSFDNLTPLYPMEKFKLETTPDNLTGRVLD
                     LLAPIGKGQRGMIVSPPKAGKTMMLQSIANSITANHPDVILMVLLIDERPEEVTDMQR
                     SVKGEVVASTFDEPATRHVQVARMVMEKAKRLVEYKKDVVILLDSLTRLARAHNTVCP
                     PSGKLLSGGIDANALQKPKRFFGTARNIEEGGSITIIATALIDTGSRMDEVIYEEFKG
                     TGNLEIHLDRTIMERRVFPAIDISKSGTRKEELLLPKEQLDRIWILRKLLSPLNPIDS
                     IEFLLDKLSKTRNNTEFLESMNK"
     gene            2535..2759
                     /gene="rpmE"
                     /locus_tag="PLT69_07200"
     CDS             2535..2759
                     /gene="rpmE"
                     /locus_tag="PLT69_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOJ14260.1"
                     /translation="MKKGIHPEYFETTVTCACGNSFVTRSTKKDIRVEICSACHPFYT
                     GQQRLTSTAGKAELFRRKYGKYLDKEKQGK"
     gene            2787..>3468
                     /locus_tag="PLT69_07205"
     CDS             2787..>3468
                     /locus_tag="PLT69_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOJ14261.1"
                     /translation="MLERLREIERRYEEISSLLADPEVLKDPTRYAELAKEHANLGGI
                     VEPYGRLKEILASLEESSSMLEDPDPEIRSLAREELAALEREKELLETRIRCLLLPRD
                     PNDERHVILEIRAGTGGDEAALFAADLFRMYCRYAEEKGWDVEILSANETGIGGFKEV
                     IAGIRGRGAYSRLKYESGVHRVQRVPVTEASGRIHTSAVTVAVLPEAEEVDVAIDPND
                     LRIETFRAS"
BASE COUNT          781 a         1042 c         1058 g          587 t
ORIGIN      
        1 cgttcatgaa gggcctcccg aacaggccgc ccgtgctcct caagaagggc gaagggatcg
       61 ccggcaaggt ggcccagacg gccaagacga tctacatccc cgacgtgacg aaggatccga
      121 acttctgtga caagggcaca cggcaggaag ggtcgctcgc atgcgtgccc atcatttccg
      181 agggcgagtg cataggcgtg atcaatttca gggacaacgc ccccggcgcc ttcgggccca
      241 acgacctcag gtttttcgag ctcatcgcgg accagctgtc gatcacggcg tcgctcgtac
      301 gcacctacca ggatctcctc gatctcgaga agaagaggat gaacctgagc aggtatttct
      361 cgaagggtct cgccgagcac ctcctgacgg acagccgcat gacccgcctc gggggggaga
      421 agaaagcggt gacggtgctg ttcgcggaca tcgtcgggtt cacgtcgctg gtggagaaga
      481 accccgtgga aaaggtggtg gtggtgctca acctcttctt cgagaccgtg gtccccatca
      541 tcttctcgca tggagggatg ctcgataagt tcctgggtga cggtctcatg gcggtcttcg
      601 gcatcccgga tgccgacgac ctcgaccccg tgagggcggt caggtgcgcg acagacatcc
      661 agcgcgccat ggcggggctc cgtgaccgat tcgagcggga ggggtatttc tccatcgagg
      721 tcggcatcgg catatcctcc ggggtcgtgg ttgcgggaaa cataggcacc ctcgagcaca
      781 tgaactacac ggtcataggg gagacggtga atctggctca gcgcctcgag tccatcagcg
      841 atcccgggca gatcatcgta agcgacgtca cgaggcggct catccccgat cacgcgccga
      901 tcgacgtgtc gttcgagccc atgggcacca tgcgcctcaa gggcatcgac agggagatcg
      961 cgcccttcag ggtcgtatac tgacaggcaa cgaccgtggg cggccctggg tcgtcgggtg
     1021 gccgcacacg gccacctttt caggccggcg ggtggatgga cggtgtgccc cctgccgaaa
     1081 cacaacgggg agatcccgtg gcgatcgaca aggaggcacc atggtgacct cgtgcaagga
     1141 cacggcatgt ttttgccctt gacttcctcg tatgttttgt ttagtccatg accgtaccga
     1201 tacttctgtt cgtgggccct ctcgaagcga tagatctttc cacgtcagct cataccaccg
     1261 aaaggacatg gcaatatgca tttagaagac ctcaagaaga tggatcccaa ggaactcctc
     1321 gaaacggcca acgccctcga gatagagggt tcggccacca tgaacaagca ggagctcata
     1381 ttcgccattc tccaggcgca ggcggagaag gacgtatcga tctccggcaa gggtgtcctc
     1441 gagatccttc ctgacggttt cgggtttctc cgctccgcgg actacagcta tctgcccggg
     1501 cccgatgaca tctacgtctc cccctcgcag atccgccgct tcaacctgaa gaacggcgac
     1561 gaggtcaacg gtttcatcag gccgcccaag gacaacgagc ggtacttcgc cctcctgaag
     1621 ctcgagagca tcaacatgga agatcccgaa gcggcgaagc accgggtatc cttcgacaac
     1681 ctgacccctt tgtaccccat ggagaagttc aagctcgaga ccacgcccga caacctcacc
     1741 ggcagggtgc tcgacctcct ggcccccata ggcaagggac agaggggcat gatcgtgtca
     1801 cccccgaagg cgggcaagac catgatgctc cagagtatcg ccaacagcat cacggccaac
     1861 caccccgacg tcatcctcat ggtgttgctc atagacgagc ggcccgaaga ggtcacggac
     1921 atgcagcgtt ccgtcaaggg cgaggtggtg gcctccacct tcgacgagcc agccacgaga
     1981 cacgttcagg tcgcccgcat ggtcatggaa aaggccaaga ggctcgtgga gtacaagaag
     2041 gacgtggtga tcctcctcga ttctctcacg aggctcgctc gcgcacacaa caccgtttgc
     2101 ccgccgtcgg gcaagctcct ctcgggcggc atcgacgcca acgccctcca gaagccgaag
     2161 aggttcttcg gtacggcgcg caacatcgaa gagggcggga gcatcaccat catcgccacc
     2221 gccctcattg acaccgggtc gaggatggac gaggtcatct acgaggaatt caagggcacg
     2281 ggcaacctgg agatccacct ggaccgcacg atcatggagc gcagggtgtt tccagccatc
     2341 gacatctcca agtcagggac caggaaggaa gagctgctcc ttcccaagga gcagctcgac
     2401 cgcatatgga tcctccgtaa gctcttgagc ccgctgaacc ccatagacag catcgagttt
     2461 ctgctggaca aactgagcaa gacacggaat aataccgaat tcttggaatc aatgaacaag
     2521 taggagcaag ggaaatgaag aaagggatcc atccggagta cttcgagacg acggtcacct
     2581 gtgcatgtgg aaacagcttc gtaacgcgat ccacgaagaa agacatccgc gtggaaatct
     2641 gcagcgcatg ccaccccttc tataccgggc agcagcggct gacctcaacc gcagggaagg
     2701 cggagttgtt caggcggaag tacggcaagt acctggataa ggaaaagcag ggcaagtaag
     2761 ccaaccttcg cccccatcga gggaacatgc tcgaacgttt gagggagatc gagaggcgat
     2821 acgaagagat ctccagtctc ctcgcagacc cagaggtact caaggacccc acccgttacg
     2881 ccgagctggc caaggagcat gcgaaccttg gcgggatcgt ggagccctat ggaaggctca
     2941 aggagatcct cgcctctctc gaagagagct cctccatgct cgaagacccg gatcccgaga
     3001 tacgctccct cgcaagggaa gagctggcgg ctctcgagcg ggaaaaggag cttctcgaga
     3061 cgaggatcag gtgcctgctc ctgcccaggg acccgaacga cgagcgccac gtgattctcg
     3121 agatcagggc gggcacaggg ggcgatgaag ccgccttgtt cgccgccgac ctcttcagga
     3181 tgtactgcag gtacgccgaa gaaaagggct gggacgtgga gatcctgagc gccaacgaga
     3241 cgggcatagg cggcttcaag gaggtcattg ccgggatcag gggcaggggc gcttactcga
     3301 ggctcaagta cgagagcggc gtgcaccggg tgcagcgggt acccgtaacg gaggcgagcg
     3361 gcaggataca cacctcggcc gtcaccgtgg ctgtcctgcc cgaggccgag gaggtggacg
     3421 tcgccatcga tccgaacgac ctgcgcatag agaccttcag ggcctcgg
//
LOCUS       DAOSCR010000260         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17430_length_3451_cov_3.300942, whole genome shotgun sequence.
ACCESSION   DAOSCR010000260 DAOSCR010000000
VERSION     DAOSCR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17430_length_3451_cov_3.300942"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..851
                     /locus_tag="PLT69_07210"
     CDS             6..851
                     /locus_tag="PLT69_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOJ14262.1"
                     /translation="MKIREKTPQKQHAQEIEALSRRLEGIRHIVMVMSGKGGVGKTTV
                     AANLSAALAREGYSVGILDGDIHGPNIPKILGVEGRTLSGNAEGDIVPVEVAKNLRLA
                     SVGNLLENQDQPVVWRGPMKHSMLRHFLGGVAWGELDFLVVDLPPGTGDEPLSIAQLI
                     REASVDKVPYAIVVTTPQDIALLDSRKSVEFARTLDLRLLGIIENMSTFSCPHCGHAI
                     DLFKVGGGERAARDLGVPFLGAIPIDPTVVRDTDDGRPFMFHNMSSAAGMAFGRIVEK
                     VLAAV"
     gene            864..1739
                     /locus_tag="PLT69_07215"
     CDS             864..1739
                     /locus_tag="PLT69_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOJ14263.1"
                     /translation="MHLRLECIPCLVRHAYDISVHAASDAGREGFIREVLALVSRMDF
                     ARVPPLAARDIYDLLEKTTGVRDPFKDVKDASNRKALELYPWLEGIVASSPDPIDTAL
                     RVALAGNMVDYGRSSADGIDMRASVLRYLEADFAVDHRRRLREALEGARTVLYVLDNA
                     GEIVLDRLLVEVLGARRVVCAVRSSPIINDATMDDARAAGLADMCRVIPSGSGASGTP
                     LESCSDEFREVFSKADVVISKGQGNFETLFEEDRDIFHLFVAKCPVISREIGVAEGSA
                     LAFFNSALKRERKGD"
     gene            1781..>3451
                     /locus_tag="PLT69_07220"
     CDS             1781..>3451
                     /locus_tag="PLT69_07220"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOJ14264.1"
                     /translation="MKKILIVASLVAALVVGILAALPFAIDLNRHKASLIALIAKYTD
                     RRVDFERIDLTVLTGLGARIKGLRVSDDPRFSAGDFLSLDEVRVKVALLPLLRKEIRV
                     RELSFVRPSVMLVRGEDAKLNVSTLVKPGPGKKEEGKESVLGGVMAAGLKVKDGKLVF
                     VDKRPGKAGGAITLSDVDLDVDTMDPARPTGFTLSASLMGTAAQNLNVMGSVGPLPGR
                     TGAGPLPIDMQVVIDPLMLGSLPIGKNPLAGGAKVKASAKGDLAGVVDARVEADFTGL
                     SLAGRKGGVDASCSFRAAYDGARGGLLVKEGAFTTPGGKGNFTIQLKDMKTDPSLSLN
                     VSSASFAPAPLLEGLGLGRAQALGKVKVSGPMAFRIEAHRSKGSIRVRASVDMRQAAL
                     AYGTFFDKPASVPMAASLRMAGTPETISIESFEVAIERVGIHGSGRLSKEQGGRRLKM
                     AIATESLRLADARALVPVLKKYSPEGVVVAKAEMESGAKRPFTLGLKARSDRVALVLG
                     AKGDRPAQGAKVLEAPARAELDSVDLSLDAVGQKGGPSVKGTVLSRGGV"
BASE COUNT          611 a         1068 c         1204 g          568 t
ORIGIN      
        1 tcgagatgaa gatcagggag aagacaccgc agaaacagca cgcgcaggag atcgaggcgc
       61 tctcgaggcg gcttgagggt atcaggcaca tcgtcatggt catgagcggc aaggggggcg
      121 tcggcaagac cacggtcgcg gcgaacctct cggccgccct cgcgcgggag ggctactccg
      181 tggggatcct cgacggcgac atccacgggc cgaacatacc caagatcctc ggcgtggagg
      241 gcagaaccct ctccggcaac gccgaggggg acatcgtgcc cgtcgaggtc gccaagaacc
      301 tcaggctcgc gtcggtgggc aacctcctgg aaaaccagga ccagccggtc gtctggcgag
      361 gacccatgaa gcactccatg ctgaggcact tcctgggagg cgtggcctgg ggcgagctcg
      421 actttctcgt ggttgacctc ccacccggga caggggacga gccgctctcc atagcgcagc
      481 tcatacggga ggcgagcgtg gacaaggtgc cgtacgccat cgttgtcacg accccccagg
      541 acatcgcgct cctcgactcc cgcaagagcg tcgagttcgc gcgcacgctc gacctgaggc
      601 ttctcggcat catcgagaac atgagcacct tctcatgccc gcactgcgga cacgccatcg
      661 acctcttcaa ggtcgggggg ggcgagcgtg cggccaggga tctcggggtc cccttcctcg
      721 gggcgatccc catcgacccg acagtggtca gggacacgga cgacgggagg ccgttcatgt
      781 tccacaacat gagcagcgcc gccggcatgg ccttcgggcg catcgtggag aaggtcctcg
      841 ctgctgtgtg aggctgtccg cccatgcacc tgaggctcga atgcataccc tgcctcgtcc
      901 gtcacgccta cgacatctcg gttcacgcag cctcggacgc gggccgcgag ggcttcatca
      961 gggaggtcct tgccctggtc tcgaggatgg acttcgcccg ggtgccgccg ctggcggcca
     1021 gggacatcta cgacctgctc gagaagacca ccggcgtgcg cgaccccttc aaggacgtca
     1081 aggatgcatc caacaggaag gcgctcgaac tctacccctg gctcgagggt atcgtcgcgt
     1141 cctctcctga cccgatcgac accgccctga gggtggccct ggcaggcaac atggtggact
     1201 acggcaggtc gagcgcggat ggtatcgaca tgcgcgcgtc ggtactcagg tatctcgagg
     1261 ccgacttcgc cgtggaccac aggcgccgtc tcagggaggc gcttgaaggc gcgcgtaccg
     1321 ttttgtacgt cctggacaac gcgggcgaga tcgtcctcga caggctgcta gtggaagtgc
     1381 tcggtgcccg aagggtggtg tgcgccgtaa ggtcttcccc gatcatcaac gacgcgacca
     1441 tggatgacgc cagggcagcc gggcttgccg acatgtgcag ggtcatcccg agcggctcgg
     1501 gtgcatcggg cacaccgctg gaatcttgtt ccgacgagtt cagggaggtc ttctccaagg
     1561 cggacgtcgt gatatccaag gggcagggca acttcgagac cctgttcgag gaggacaggg
     1621 acatctttca cctcttcgtg gccaagtgcc cggtgatctc gagagagatc ggggtggccg
     1681 aggggagcgc cctcgccttc ttcaactctg cgctgaaacg tgagaggaaa ggggattgag
     1741 gcgttgaggc cttttgtttc gtgatccaag gagggttgag atgaagaaga tcctgatcgt
     1801 tgcctctctc gtggcggccc ttgtcgtggg gatcctcgcg gcgctgcctt tcgcgataga
     1861 cctgaacagg cacaaggcct ccctcatcgc gctcatcgcc aagtacacgg acaggcgcgt
     1921 ggacttcgag cggatcgacc tcacggtgct cacgggcctc ggcgcccgca taaagggact
     1981 gcgggtctcg gacgacccgc gcttttccgc aggcgacttt ctctccctgg acgaggtgcg
     2041 cgtgaaggtg gcgctgctgc cccttctgag gaaggagatc agggtcaggg agctctcgtt
     2101 cgtcaggccc tcggtcatgc tcgtgagggg cgaggacgcc aagctcaacg tctccacctt
     2161 ggtgaagccg ggcccgggaa agaaggaaga agggaaagag agcgtgctcg gaggggtcat
     2221 ggcggcgggg ctcaaggtga aggacggaaa gctcgtcttc gtcgacaaga ggcccgggaa
     2281 ggccggtgga gcaatcaccc tttccgacgt ggacctggat gtcgacacga tggacccggc
     2341 aaggcccacg gggttcaccc tgtcggcgtc gctcatgggg accgccgccc agaatctcaa
     2401 tgtcatgggg agcgtcggtc cgcttcccgg gcgcaccgga gcgggacctc tgccgatcga
     2461 catgcaggtc gtgatcgacc cgctcatgct cggctcgctg ccgatcggga agaacccgct
     2521 cgccggcggg gcgaaggtca aggcatcggc caagggtgac cttgcgggcg tggtcgatgc
     2581 ccgggtggaa gcggacttca cggggctctc gttggccggt cggaaggggg gggtcgatgc
     2641 ctcgtgctcg ttcagggcgg cctacgacgg ggcgcggggg gggctcctcg tgaaggaggg
     2701 cgccttcacg acgccaggcg gcaaggggaa cttcacgatc cagcttaagg acatgaagac
     2761 cgacccgtca ctgagcctga acgtgtcgtc ggcctcgttc gccccggcgc ccctgctcga
     2821 gggcctcggc ctggggcggg cccaggccct cggaaaggtc aaggtgtcgg gccccatggc
     2881 cttcaggatc gaagcccaca ggtcaaaggg ctcgatcagg gtgagggctt cggtggacat
     2941 gcgccaggcc gcccttgcct acgggacgtt cttcgacaag ccggcatcgg tccccatggc
     3001 cgcatccctg cgcatggcag gcacgccgga gacgatctcc atcgagtctt tcgaggttgc
     3061 gatcgaacgg gtcgggatcc acggatcggg caggctttcc aaggagcagg ggggcaggag
     3121 gctcaaaatg gcgatagcca ccgagtctct caggctcgcg gatgcacggg cgctggtccc
     3181 cgtcctcaag aagtacagcc ccgaaggtgt cgtcgtggcc aaggccgaga tggagtccgg
     3241 ggcgaagagg ccgtttacgc tcggcctcaa ggcgaggtcc gaccgcgtgg ccctggtgct
     3301 cggcgccaag ggggaccggc ccgcccaggg cgcgaaagtc ctcgaggccc cggccagggc
     3361 cgagctcgac tcggtggacc tctccctgga cgccgtcggg cagaagggcg gtccttccgt
     3421 aaagggcacg gtcctctcga ggggaggggt g
//
LOCUS       DAOSCR010000261         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17485_length_3442_cov_3.850901, whole genome shotgun sequence.
ACCESSION   DAOSCR010000261 DAOSCR010000000
VERSION     DAOSCR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17485_length_3442_cov_3.850901"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLT69_07225"
     CDS             complement(<1..760)
                     /locus_tag="PLT69_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14265.1"
                     /translation="MYWDERNECIGPEDLARLQFARLRETVRRAARSPFYRAKGLGEI
                     EVRDLSDVRKLPFTTKDDLREAYPFGMLSCPLNDVVRLHSSSGTTGTPTVVYHSKKDI
                     DRWTDLVARCLYMIGVRKGDVFQNMIGYGLFTGGLGLHYGAERLGCLTIPTGPGNTKR
                     QLKFMKDFGVSVIHVIPSYALHLADEVEKLGIDPRREFPSFKYALLGAEPHTEETRRK
                     VEEFFGIQAYNSYGLSEMNGPGVAFECPEKNGMHI"
     gene            complement(956..1348)
                     /gene="secG"
                     /locus_tag="PLT69_07230"
     CDS             complement(956..1348)
                     /gene="secG"
                     /locus_tag="PLT69_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ14266.1"
                     /translation="MLYNFILVIHIIVCIGLVIAVLIQAGKGAEIGAVFGGAGSQALF
                     GTAGPVDFIKKATLVMVVVFMLTSLTLGFFNLERPSTSVMSKAPAATQQAPSGRAGTS
                     APAAGAPTGVPATPSPVGTPAQAPAGGK"
     gene            complement(1353..2108)
                     /gene="tpiA"
                     /locus_tag="PLT69_07235"
     CDS             complement(1353..2108)
                     /gene="tpiA"
                     /locus_tag="PLT69_07235"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ14267.1"
                     /translation="MRRPLIAGNWKMYKGLEEALAFARAFAPKVAHVDARDILICPPF
                     VAIHALAQVFAGTNVLLGGQNVHHEDQGAFTGEVSAPMLKSAGATHVIIGHSERRQLF
                     GETDEGVNRKVHASLRSSLSPVVCVGETLSEREAGLTLEVVGKQIERGLHGLGQEGAS
                     SLAVAYEPVWAIGTGRTATPSIAQEVHAFIREVLSKILGPQAASRIRILYGGSVKPDN
                     VDELMAEPDIDGVLVGGASLEPESFERIVKYRG"
     gene            complement(2118..3299)
                     /locus_tag="PLT69_07240"
     CDS             complement(2118..3299)
                     /locus_tag="PLT69_07240"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOJ14268.1"
                     /translation="MAVRSVSDLDVKGKRVLIRVDFNVPLDEHGSITDDARIRAALPT
                     IQHVMGKGGRVILMSHLGRPKKPDKKLSLIPVARRLSELLGTSVNMAPDCVGEKVKSM
                     VDALAPGEVLLLENLRFHPEEEKNDEGFAKELAALCDVYVNDAFATAHRAHASTAGLC
                     PYVKETAAGFLMEKEVKYITRVLTDPARPFAAILGGAKVSDKLEIVENLLEKADKILV
                     GGGMAFTFVKALGQDVGRSLVEEDLVGKAREIMEKAKARKVKFYLPVDCVCGPDPKTA
                     GQALVRTIQEMPSDLMGLDIGPATAKLFSEALDGCRTIVWNGPMGVFEVKDYALGTMS
                     VAKAVASSPGLTIVGGGDTDAALHASGLSDKVSFVSTAGGAFLEMLGGRVLPGVEALE
                     K"
BASE COUNT          555 a         1191 c         1046 g          650 t
ORIGIN      
        1 agatgtgcat gccgttcttc tccgggcact cgaaggccac gcccggaccg ttcatctccg
       61 agaggccgta ggagttgtac gcctggatgc cgaagaattc ctccactttc ctcctcgtct
      121 cctcggtatg cggctccgcg cccaagaggg cgtacttgaa cgaggggaat tcacgtctcg
      181 ggtcgatgcc gagcttctcc acctcgtcgg ccaggtgaag ggcatagctc gggatcacgt
      241 gtatcacgct caccccgaag tccttcatga acttgagctg gcgtttcgtg ttcccgggcc
      301 ctgtggggat ggtgaggcac ccaaggcgtt cggcgccgta gtggagaccg agcccccccg
      361 tgaagaggcc gtatccgatc atgttctgga acacgtcacc cttgcgcacg cctatcatgt
      421 agaggcagcg cgccacgagg tcggtccagc ggtcgatgtc tttcttcgaa tggtagacca
      481 cggtgggcgt gccggtggtc ccggaggagg agtggagcct caccacgtcg ttgagggggc
      541 agctcagcat cccgaaggga tacgcctcgc gaaggtcgtc cttggtggtg aacggcagct
      601 tcctgacgtc cgacaggtcc ctcacctcga tctcgccgag gcccttggcc cggtagaagg
      661 gggatcgagc cgccctccgt accgtctccc tcagccgggc gaactggagc cgggcgagat
      721 cctcaggccc tatgcactcg tttctttcgt cccagtacat gttcgttccc cctgtggaaa
      781 gctgcggcgc catgcgagac cggcacttcg agctccatgc cacccggtcc agggtcatgc
      841 cctgccgggg gaagagggcc cggccagctg ctcaagcccc gccccccgtg ccgggagcca
      901 ccccacaccc cgcggtcggg gggaaacggg tacgccccct cgaccgggca gggccttact
      961 tgcccccggc aggggcctgc gccggcgtgc cgaccggaga gggtgtcgcg ggaacacccg
     1021 tcggtgcacc cgcggcggga gcggacgtgc ccgcccgccc cgagggcgcc tgctgcgtcg
     1081 ctgcaggagc cttggacatg acgctcgtgc tcggcctttc cagattgaag aacccgagcg
     1141 tgagcgaggt gagcatgaac acgacgacca tgacaagggt tgccttcttg atgaaatcca
     1201 cgggtccggc cgtcccgaag agggcctggc tcccggcgcc gccgaagacc gcccctatct
     1261 cggcgccttt ccccgcctgg atgagcaccg cgatgacaag gcctatgcac acgataatgt
     1321 ggatgaccag gatgaagttg tagagcatgt ctctatcccc tgtacttcac tattctctcg
     1381 aacgactcgg gctcgaggct cgcgccccct actaagacgc cgtcaatgtc gggttcggcc
     1441 atgagctcgt ccacgttgtc gggcttcacg ctgccgccgt agaggatcct tatccgtgag
     1501 gccgcctggg ggccgagtat cttcgacaag acctcccgga tgaaggcgtg aacctcctgg
     1561 gcgatcgagg gcgtggccgt cctgcctgtc ccgatcgccc acacgggctc gtaggcaacg
     1621 gccagagaag aagctccctc ctggcccagg ccgtggagcc ccctctcgat ctgctttccc
     1681 accacctcga gggtcaggcc ggcctcccgc tcgctcaagg tctcgcccac gcataccacg
     1741 ggcgagagcg aggagcgcaa ggacgcatgg accttcctgt tcaccccctc gtcggtctcc
     1801 ccgaacagct gcctgcgctc cgagtgccct atgatgacgt gcgtcgcccc cgcgctcttc
     1861 agcatgggcg cgctcacctc ccctgtgaat gcgccctggt cctcgtggtg gacgttctgg
     1921 ccgccgagga gcacgttcgt gcccgcgaag acctgcgcca acgcgtggat cgccacgaag
     1981 ggcgggcaga tgaggatgtc cctcgcgtcg acgtgggcca ccttcggcgc gaatgcgcgg
     2041 gcgaaggcca aggcctcctc caggcccttg tacatcttcc agtttcccgc tatcagcggc
     2101 cttctcatgc gtcttcccta cttctcgagc gcctccacgc cgggcaggac ccggccgccc
     2161 agcatctcga ggaaggcgcc ccccgccgtc gagacgaagc tcaccttgtc ggacaggccg
     2221 ctcgcgtgaa gtgcagcatc tgtatcaccc ccgcccacga tggtcaagcc cggcgaggaa
     2281 gcgacggcct ttgcgaccga catggtgccc aaggcgtagt ccttcacctc gaagaccccc
     2341 atggggccgt tccagacgat cgtcctgcac ccgtccaggg cctcagagaa gagctttgcc
     2401 gtcgcggggc cgatatccag ccccatgagg tccgagggca tctcctgtat ggtcctgacg
     2461 agggcctgcc ccgctgtctt gggatccggg ccgcacacgc agtccaccgg gagatagaac
     2521 ttcaccttcc gggcttttgc cttctccatg atttccctgg ccttcccgac gaggtcttct
     2581 tccacgaggg acctgcccac gtcctggccg agcgccttca cgaaggtaaa ggccatgccg
     2641 ccgcccacga ggatcttgtc cgccttctcg aggaggttct cgacgatctc gagcttgtcg
     2701 gacaccttgg cccccccgag gatcgccgca aagggtctcg ccgggtccgt cagcaccctc
     2761 gtgatgtact tgacctcctt ctccatgagg aagccggctg cggtttcctt cacgtacggg
     2821 cacagccccg cggtggaggc gtgggccctg tgggccgttg cgaaggcgtc gttcacgtac
     2881 acgtcgcaca gggcggccag ttccttcgcg aacccctcat cgttcttctc ctcctcgggg
     2941 tggaaacgca ggttctcgag caggagcacc tcaccggggg ccagcgcgtc gaccatggac
     3001 ttcaccttct cgccgacgca gtccggcgcc atgttgacgc tcgttcccaa gagctcggaa
     3061 agcctcctgg ccacagggat gaggctgagc ttcttgtcag gcttcttggg cctgccgagg
     3121 tgcgacatga ggatcacgcg cccgcccttg cccatgacgt gctgtatggt gggcagcgcc
     3181 gccctgatcc gcgcgtcgtc cgtgatcgat ccatgctcgt cgagcgggac gttgaaatcc
     3241 accctgatca agaccctctt ccccttcacg tcgagatccg agacggaacg tacggccatg
     3301 gcaatccctc cccgtcttgt gtagtgtgca tcggatggat gcttcccggt ccatcggctg
     3361 gatgtgctcc gagttccccc cggcctcccg gccgacgcgg catattgttc ataccacaaa
     3421 gctccgatat tgtatatgtg tt
//
LOCUS       DAOSCR010000262         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17546_length_3433_cov_3.965364, whole genome shotgun sequence.
ACCESSION   DAOSCR010000262 DAOSCR010000000
VERSION     DAOSCR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17546_length_3433_cov_3.965364"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLT69_07245"
     CDS             complement(<1..664)
                     /locus_tag="PLT69_07245"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOJ14269.1"
                     /translation="MTRVLTVIRLIGRGVRSHPLGSLLTLGACSFASCLFALALLAVG
                     AAEKAASLPVSSGSMVAYVKEGVPRTRVPEMEKALRSLPQVRSFTFVPREKGLEKMRS
                     WLGRKSPLVEDVDPEILPDAFEITLGREYAHRPEDVSRAVAAIPGIGDVRWRKGLIAV
                     LASSYDDIAAAALALGASLGLTLGLVVFLSIRTSIASRSQEVAVMRTLGAPGSFIFAP
                     YVV"
     gene            complement(680..1330)
                     /locus_tag="PLT69_07250"
     CDS             complement(680..1330)
                     /locus_tag="PLT69_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ14270.1"
                     /translation="MITFENVTKVYHDTVYAVRDISLVIEKGSLCFIVGPSGAGKTTL
                     LKLVYGLERPSEGTVSVFGSDLSRIPRSRLQAIRRHTGFVFQDYRLVEDWTVYQNAAI
                     PLEILGKSPLYVRSRVWRMLKWIGLTHKVYERARNLSGGERQRLALVRAIIHDPDIVL
                     ADEPVGGQDETTARYLMNVFYKLHRKGTTIVIASHRSDPLMDHARIVRMEHGRILE"
     gene            complement(1389..1826)
                     /locus_tag="PLT69_07255"
     CDS             complement(1389..1826)
                     /locus_tag="PLT69_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ14271.1"
                     /translation="MSAKTLKDLRKAKGLTMGRLAELSGVSAKTISQYEKNPPARPSR
                     KALSKISEALGVSTDELTACIYPSKAAGKKAEQEPYAGDVLTLEDAHVSRLVRLIDKE
                     LEELRFILMDTAELADDHPSIARYLAMVGEDIDLLLDIRARFA"
     gene            2165..3286
                     /gene="flhB"
                     /locus_tag="PLT69_07260"
     CDS             2165..3286
                     /gene="flhB"
                     /locus_tag="PLT69_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOJ14272.1"
                     /translation="MSAGAGTVLAFTPRMPSENENGQQRTEQPTSKRLREAREKGQVC
                     KSVEVSTALLFLASVVAFYLYVPTVGRNLHGVVAEYVGNTSMWDGSKESVVFIFRRAC
                     LELGLMTLPLLVAFLVVGLASNILQVGFVASPEAIVPKLSKLNPLKGFKNRFMSTRSL
                     EQLLKTLVIMAVVVWVSYRALRREMAAYPPLLNADATVIVLTFFRSTMRFLLDVLWVF
                     VVIAAADYGFQKWQFTRDLMMTRQEVRDEFKNTEGNPLIKSKIRSIQMHMARRRMMKA
                     VPKADVVITNPTHLAVALVYDRTRMAAPTVVAKGAGEVALRIREVARGAGVPVVENKP
                     LAQTLYKSLDIGDVIPEELYKAVAEILAYVYRLKRKAVM"
BASE COUNT          577 a         1086 c         1150 g          620 t
ORIGIN      
        1 ccacgacgta cggggcgaag atgaaggacc cgggtgcgcc gagggttctc atgacggcca
       61 cctcctgcga cctggacgcg atgctcgtcc tgatcgagag gaagaccacg aggccgaggg
      121 tgaggccgag ggacgcgccg agggccagtg ccgcggccgc gatgtcgtcg taggaggatg
      181 ccaggacggc gatgaggccc ttcctccagc ggacgtcccc gatgccgggg atcgccgcga
      241 cggcgcggga gacgtcctcg ggcctgtgcg cgtattccct gccgagggtg atctcgaagg
      301 cgtcggggag gatctccggg tccacgtcct cgacgagcgg gctctttcgg ccgagccacg
      361 accgcatctt ctcgagcccc ttctccctgg ggacgaacgt gaaggacctg acctggggca
      421 acgagcgaag ggccttctcc atctcgggga cgcgggtcct cggcacgccc tccttcacgt
      481 aggcgaccat ggagccggaa gacactggca ggctcgcggc cttctcagcc gcgcccacgg
      541 cgaggagggc gagggcgaac aggcacgagg cgaacgagca cgccccgagc gtaagcaggc
      601 tccccagggg atgcgaccgt accccgcggc ctatgagcct gatcaccgtg agcaccctcg
      661 tcatgccttt gacgccccct cactcgagga tccgcccgtg ttccatgcgg acgatgcgcg
      721 cgtggtccat gagcgggtcg ctgcggtggc ttgcgataac gatcgtcgtg cccttcctgt
      781 ggagcttgta gaacacgttc atgaggtacc tcgccgtggt ctcgtcctgg cctcccacgg
      841 gctcgtcggc aagcacgatg tcagggtcgt ggatgatcgc ccgtacgagc gccagccgct
      901 ggcgttcccc gccggagagg ttcctggcgc gctcgtagac cttgtgcgta agcccgatcc
      961 acttgagcat ccgccagact cgcgacctca cgtagagagg cgacttgccg aggatctcca
     1021 gggggatcgc cgcgttctgg tacaccgtcc agtcctcgac gagcctgtag tcctggaaga
     1081 cgaaccccgt gtggcgtctt atcgcctgga gccgggacct ggggatcctc gagaggtcgc
     1141 tgccgaagac gctcaccgtg ccctccgagg gtctctcgag cccgtagacg agcttgagca
     1201 gcgtcgtctt gccggccccg gaggggccga cgatgaagca gagggacccc ttctcgatga
     1261 cgagcgagat gtcccgtacg gcgtagacgg tgtcgtggta gaccttggtg acgttctcga
     1321 acgttatcat gggacgcctg ccgccccgcc aatgccggcg ctttgtgcga ggggccggac
     1381 cacctcgctc aagcgaaccg cgccctgatg tccagaagca ggtctatgtc ctcgcccacc
     1441 atggccaggt atctggcgat ggaaggatgg tcgtcggcga gctcggccgt gtccatgagg
     1501 atgaacctga gctcctcgag ctctttgtcg atgagcctca ccagcctcga cacgtgggcg
     1561 tcctcgaggg tgagcacgtc gcccgcgtag ggttcctgct ccgccttctt tcccgcggcc
     1621 ttggacgggt agatgcacgc ggtgagctca tccgtcgaga caccgagcgc ctcgctgatc
     1681 ttggagaggg ccttcctgct cggccgtgcg ggcgggttct tctcgtactg cgagatcgtc
     1741 ttggccgaca cgccgctcag ctcggccagc ctgcccatgg tgagcccctt cgccttgcgg
     1801 agatccttga gagtcttggc ggacaatgtg tacctccatc gaatggtgtc agtcgaaacc
     1861 gcacgatgct gatcgtcatc cctgttattt gcacaaaacg ccgggtttca caactctaaa
     1921 cagcgcgtgt aatcagaggg ttcggctgca gggtgccctg acgcgactcc cgccgagggc
     1981 gttctccctg caagtgacgg aaatcgcggg gggtgggctg caggcgcccc gaccctctcg
     2041 ccgtgcccgg ccgtgatccg ctgagagaaa cgcagccggg tccgggcgtg gtgcatggcg
     2101 ggttcccggc ggcgggggca gccttctcac cctgggtgac agagagggaa ggggaggcgc
     2161 cctcgtgagc gcgggggctg gtacggttct cgcattcaca ccccgtatgc cgagcgagaa
     2221 cgagaacggc caacagcgta ccgagcaacc tacatcgaaa cgcctgagag aagccaggga
     2281 gaagggccag gtgtgcaaga gcgtcgaggt gtcaacggcc ctcctcttcc tggcctcggt
     2341 cgtggccttc tacctctacg tgccgaccgt cggcaggaac ctgcacggcg tggtggccga
     2401 gtacgtcggg aacacgtcca tgtgggacgg ctcgaaggag tcggtcgtgt tcatcttcag
     2461 gcgtgcgtgc ctggagctcg gcctcatgac gctgcccctg ctcgtggcgt tcctcgtcgt
     2521 ggggcttgcg tcaaatatcc tgcaggtggg gttcgtggcg agcccggagg ccatcgtccc
     2581 caagctctcg aagctcaacc cgctgaaagg attcaagaac aggttcatgt ccaccaggag
     2641 cctcgagcag ctcctcaaga cgctggtcat catggcggtc gtcgtctggg tttcgtaccg
     2701 ggcgctacgg cgcgagatgg cggcgtaccc gcccctcctg aacgccgacg caactgtcat
     2761 tgttttgacg tttttccggt cgaccatgag gttcctcctg gatgtcctct gggtgttcgt
     2821 ggtcatcgcg gcggccgact acgggttcca gaagtggcag ttcacccgcg acctcatgat
     2881 gacgaggcag gaggtcaggg acgagttcaa gaacaccgag ggcaaccccc tcatcaagag
     2941 caagatacgg tccatccaga tgcacatggc gaggcgccgc atgatgaagg cggtgccgaa
     3001 ggccgacgtg gtgatcacga acccgaccca cctggcggtc gccctcgtgt acgaccgcac
     3061 gaggatggcg gcgccaacgg tggtggccaa gggggcgggt gaggtggcgc tcaggatcag
     3121 ggaggtggcg cgtggcgccg gggttcccgt ggtcgagaac aagccgctcg cccagaccct
     3181 ctacaagtcc ctggacatcg gggacgtgat ccccgaggag ctctacaagg cggtggccga
     3241 gatcctcgca tacgtctaca ggctcaagcg gaaggcggtg atgtgacatg gcgacccagg
     3301 cgttttcgat gaggcttccc gagaaggtat cggccgacgt gttcatggcc gcaggggtcc
     3361 ttgcggttct cttcatcctg ctcgtccccc tgccgacctt cgtgctggac atcctgctga
     3421 ccttcagcat cgt
//
LOCUS       DAOSCR010000263         3412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17693_length_3412_cov_3.291332, whole genome shotgun sequence.
ACCESSION   DAOSCR010000263 DAOSCR010000000
VERSION     DAOSCR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17693_length_3412_cov_3.291332"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLT69_07265"
     CDS             complement(<1..910)
                     /locus_tag="PLT69_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14273.1"
                     /translation="MQVPINYMLNAEEIAYIVNHSRSKIFIVEDSLYPVIARHQASFP
                     SVQRWGFIPLGDSMVPDGFFDLMSEMKRMPHEEPHVDVEAEDIVQIPYTSGTESRPKG
                     AMLSHRALMSQYHSCIFDGRYDTYDVSLHALPLFHCAQLHCFLMPYLYVGATNVIMHK
                     ADPVEMIRNIEKYQITHMFAPPTVWIGILNHPDFKKHNLRSLKKAAYGASIMPVEIIK
                     QLCVAFRGLELWNYYGQTEMGPVATILKPEDQLLKPGSAGKPVLNVETRIMDDEGRFV
                     PLGTVGEIVHRSGHVMSGYLDDPEKTA"
     gene            complement(802..1158)
                     /locus_tag="PLT69_07270"
     CDS             complement(802..1158)
                     /locus_tag="PLT69_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14274.1"
                     /translation="MSNIINRDTVGDMARRAATKYGDKTAIIFRDVQLSFYDLERESR
                     RFAHLMSSYGIRKGDRVAIYAYNSHYYPISCSAWRRSVRCRSPSTTCSTRRRSPTSST
                     TRGRRSSSWKTPSTRS"
     gene            1899..2816
                     /locus_tag="PLT69_07275"
     CDS             1899..2816
                     /locus_tag="PLT69_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOJ14275.1"
                     /translation="MVKRPSDNPLSCAPCHAGIAADYAKSLHFTSAGQRHGVIPRFSA
                     KEVALFDKKVFEQSCRSCHASCGSCHVKSPPVMGISLGLIKGHAFVKKDEGKTCAFCH
                     GGRVYPEYTGEYSGVTDVHYQKGMMCVDCHTKTEMHGDGRRYLTKQDVRQRPTCTTCH
                     KDISSGKKLRVSVAHEEHRGKVSCYGCHAAGQYRNCSDCHGGEAKASPGFILGKNPRN
                     TKEITTLRLIPTVRDTFAKAGIVQERYDRVPNYWNTPAHTIRKRTDRTQSCDACHTEK
                     KYFLTVEGLPKDGSRANRGLIHAPGPITK"
     gene            2831..>3412
                     /locus_tag="PLT69_07280"
     CDS             2831..>3412
                     /locus_tag="PLT69_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOJ14276.1"
                     /translation="MERTVRMVVLAVLVIGLSIPVTLMAQDIGPVVSVDWLEQNLSNP
                     KLVVVDIRKVEEYRSGHIPGAVNVFYNILAPSKGELKNELPGDQDLRDTLGDAGISAD
                     SWVVVAGVNDTPADRFSMTRIAMTLAYAGVKNVAVLDGGTTLWAAKGKALSTDVVRPK
                     AVQFKGVFNKAFIVKKDELAQKLGKAVLVDVREP"
BASE COUNT          650 a          982 c         1122 g          658 t
ORIGIN      
        1 ccgccgtttt ctcggggtcg tcaagatacc cgctcatgac gtgacctgac cggtgcacga
       61 tctcccccac cgtcccgagg ggtacgaagc ggccctcgtc gtccatgatc ctcgtctcca
      121 cgttgaggac gggcttgcct gcggaacccg gcttgaggag ctggtcctcg ggtttcagga
      181 tggtggccac cggccccatc tcggtctgcc cgtagtagtt ccagagctct agccccctga
      241 aggccacgca gagctgcttg atgatctcca cgggcattat gctggcgccg tatgcggcct
      301 tcttgaggct cctgagattg tgtttcttga agtctgggtg gttgaggatg ccgatccaca
      361 ccgtgggggg ggcgaacatg tgcgtgatct ggtatttctc gatgttcctg atcatctcga
      421 cggggtctgc cttgtgcatg atgacgttcg tcgcgcccac gtagaggtag ggcatgagga
      481 agcagtggag ttgcgcgcag tggaacaagg ggagcgcatg gaggctcacg tcgtaggtgt
      541 cgtacctccc gtcgaagatg cagctgtggt actgggacat gagggcccgg tgggagagca
      601 tggccccctt cggtctcgac tccgtgccgc tcgtgtacgg gatctggacg atgtcctcgg
      661 cctcgacatc cacgtgcggc tcttcgtgcg gcatccgttt catctcggac atgaggtcga
      721 agaagccgtc cgggaccatg gagtccccga gcgggatgaa cccccaccgc tggaccgagg
      781 ggaaagacgc ctggtggcgt gctatgaccg ggtagaggga gtcttccacg atgaagatct
      841 tcgacctcga gtggttgacg atgtaggcga tctcctccgc gttgagcatg tagttgatgg
      901 ggacctgcac cgcaccgatc ttcgccaggc cgagcagctt atggggtagt agtgcgagtt
      961 gtaggcgtag atggccaccc ggtcaccctt cctgatgccg taggagctca tgagatgggc
     1021 gaagcgcctt gactcgcgtt ccaggtcgta gaaagagagc tgcacgtcac ggaagatgat
     1081 cgccgtcttg tcgccgtact tggtcgccgc cctcctcgcc atgtcgccga cggtgtcccg
     1141 gttgatgatg ttgctcatcg ctggttcctc ccctggtgtt tcgctcgcgg ccgcggcgcg
     1201 gccgggttgc gcatggacag cataccaaac atcgactgag atatcaatgt cggcgattcc
     1261 gggggccgac gtgttcggcg ggatcctgcc tggtgcaggc atccggcgga gtaggcctcc
     1321 ttcctttcac tcccgccggt gccgaggcgt ttccccgggg taacgctcgg cgatggtcat
     1381 ggggtggcgg ccgggtatgc ggcaaggagc cggcggtggg gagtgtcgcc ctggctgctg
     1441 ttttcatggc agggcattga ctgcgccggg tgagcctctt attgagaaga taccgagatg
     1501 ctttgccttg tcctcggctg gcgcgagcgg acaaggccgc ttcatccgcg acaagatatg
     1561 cgaacggggg tgctcgcttg aggctgttcg ttgcgtgggc ggtgtgtgtg acggtgtgtg
     1621 ctctttcgcc tctcgcggcg ttggccgcag aaaagggcac ctgtgcagcc tgccacacga
     1681 accccgagac cctcagggcc ctcgttgtcg caccgaaact ccaggtcgag ggggaggggt
     1741 gagcgggttc gcccccgccg gtccgggcgg aggaatatta caaggggtat ctcgttgacc
     1801 catccatcct gggctctgac ccgcacctct cgaagggatg cgtgttctgc cacaaaggcg
     1861 acggcacggc aacggaacgt gagagagccc atgcttccat ggtaaagaga ccgtcggaca
     1921 accccctttc ctgtgcaccg tgccatgcgg gtatcgctgc agactatgcg aaatctctcc
     1981 acttcacctc ggccggacag cgccacgggg tgatccccag gttctcggcc aaagaggtcg
     2041 ctttgttcga caagaaggtg ttcgagcagt cgtgcaggag ttgccacgca tcctgcggat
     2101 cgtgccatgt caagtctccg cccgtcatgg ggatcagcct cgggctcatc aagggccacg
     2161 cgttcgtcaa gaaagacgaa ggcaagacgt gtgcattctg ccacgggggg agggtatacc
     2221 cggaatatac gggagagtac agcggggtaa ctgatgtcca ttaccagaag ggcatgatgt
     2281 gcgttgactg ccacacgaag accgagatgc acggtgacgg caggcgatac ctcaccaagc
     2341 aggacgttcg gcagagaccg acgtgcacca cgtgccacaa ggacatctcg tccggcaaga
     2401 agcttcgcgt atcagtggcc cacgaggaac accggggcaa ggtgagttgc tacgggtgcc
     2461 atgccgccgg gcaatacagg aactgttcgg actgccacgg cggcgaggcg aaggcaagcc
     2521 cggggttcat cctggggaag aaccccagga acacgaagga gataaccacc ctcaggctca
     2581 tcccgaccgt gagggacacg ttcgccaagg cggggatcgt gcaggagagg tatgacaggg
     2641 tgcccaacta ctggaacacg cctgcgcaca ccatcagaaa acgcacggac aggacgcaat
     2701 cgtgcgatgc gtgccatacc gaaaagaagt acttcctcac cgtcgagggc ttgccgaagg
     2761 acggatccag ggcaaacagg ggcttgatcc atgcccccgg tcccataacg aaataaccga
     2821 ggaggagctt atggaaagaa cagtcaggat ggtggtgttg gcggtcctgg tgatcggcct
     2881 gtcgatcccc gtaacgctga tggctcagga catcggcccc gttgtctctg tggattggct
     2941 cgaacagaac ctgtcgaacc cgaaactcgt cgtggtggac atccgcaagg tcgaggaata
     3001 ccgctcgggg cacatacctg gtgccgtcaa cgtgttctac aacatcctcg ccccttcgaa
     3061 gggggaactg aagaacgagt tgcctggaga ccaggatctt cgcgataccc tgggcgatgc
     3121 cggcatctcg gccgattcgt gggtcgtggt ggcgggcgtc aacgacacac ccgccgacag
     3181 gttcagcatg acccgcatag ccatgacgct cgcctatgcg ggggtgaaga acgtcgccgt
     3241 gctcgacggc ggcaccacgt tgtgggccgc gaagggaaag gccctgtcga ccgacgttgt
     3301 aaggcccaag gccgtgcagt tcaagggcgt cttcaacaag gcgttcatcg tgaagaagga
     3361 cgagctcgcc cagaagctgg gcaaggccgt gctcgtcgac gttcgtgagc cc
//
LOCUS       DAOSCR010000264         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17715_length_3409_cov_3.021765, whole genome shotgun sequence.
ACCESSION   DAOSCR010000264 DAOSCR010000000
VERSION     DAOSCR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17715_length_3409_cov_3.021765"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..732
                     /locus_tag="PLT69_07285"
     CDS             238..732
                     /locus_tag="PLT69_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ14277.1"
                     /translation="MKDTRTDTSQLYKYFSSNPTECPYYLVTRASLAATSLFKKAFAE
                     EGLTDLKPAYLGTLLSLWKDDGLKIIELGRRAGLEPSTMTGLIDRMEKDGLVSRVPDP
                     EDRRVIRVFLTSEGRDIELSVASVVEKVMAKILSGFEDDEVALLKESLRKMLSNIHQE
                     NADE"
     gene            725..1771
                     /locus_tag="PLT69_07290"
     CDS             725..1771
                     /locus_tag="PLT69_07290"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ14278.1"
                     /translation="MSRPIPGHVGYACSYTPLALVLASGFTPYRVLPVTSGPDQAGLH
                     LHDNLCPHVKRILDRALAGDLPDMAGMIFMNSCDPMRRLADAWTKVKGGNVAMVDLPV
                     NGLSRSVDFFARELGRLAGILESWGGDPVTPKRLASGIAATNEIANALAGLENHPPCG
                     LGGSVIYEAYTACATSPPAEALAFVKGIVGSRSTSMADSDAPKVFVFGNVLVGSEAIN
                     LIEACGARVALEDTCMGSRMFRRMEMAEGEVSFTALARAILSGKACARTFDPARPGGI
                     ALDMLEAARGCGARGAICHTAKFCDPYLSRLPHIREVFRKEGMPLLVLEGDCTARSIG
                     QHSTRIEAFLEMLR"
     gene            1776..3014
                     /locus_tag="PLT69_07295"
     CDS             1776..3014
                     /locus_tag="PLT69_07295"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ14279.1"
                     /translation="MLGQYFESLESGILSSIEKDGGKASPRKLYALEIARLGRRLYDG
                     GHKVAWCGVAAPFDLLNAMGLTSCFVEFVGAMLASTGMAGNIIEQADHWGYSSDACSY
                     HRSVTGAALAGLMPVPEILVGTTCPCTGGLGVIENLARHFGKPLFVLNIPQNDTVEDV
                     RYLADQLRDLVGFASGHTGIPFDQERLTRAIENTNAARELFVEIFDLASRVPSPASSK
                     DLNNFMISLALLLGTRECVDLARAFKDEFARRVETGSAGVKGEEIRLLWIQNRIQFKN
                     PIEKMLEDEFRACIVIDELNDIYWDPIDPDDPFEGIARRCILIPFNGPSSRRIEHLKK
                     LALTYRVHGAINPCNWGCRQGTGMRGLIEAGLREVGVPVLNLEVDCVDDRKFTEGQFR
                     TRIEAFMEMIGSNRSSRRDS"
     gene            3028..>3409
                     /locus_tag="PLT69_07300"
     CDS             3028..>3409
                     /locus_tag="PLT69_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ14280.1"
                     /translation="MYYLGIDIGSLSCDAVLLDGHGRVVADAIVPTGAKNAEAIALAT
                     ELVLNRAGATREKVKAVVSTGYGRDRVEGRLASVTEITCHARGMRHLNEKARILIDIG
                     GQDSKCILMSETGAVVNFAMNDKCA"
BASE COUNT          679 a         1081 c         1103 g          546 t
ORIGIN      
        1 gtcgagatgc cccagccggc gcgaagacgg gatgaacgtc gaggtgccga tgcctgctgc
       61 cggcttttcc cccagcccac gcgaacacgt catgaacgaa ggcctccatc gttactgacg
      121 tcaacaccca gggccagggg gacatccgca tgcagaatgt tcgcttgaag aatctacttg
      181 acaataattt tcgtttacga aatatttgcc tgagaaagga acaggtacgg acctcgcatg
      241 aaagacacca ggacagacac ctctcaactc tacaagtact tctcgtcgaa ccccacggag
      301 tgcccctact acctcgtgac gcgcgcctcc ctagcagcca cgtcgctctt taagaaggcc
      361 ttcgcagagg aaggcctgac tgacctcaag cccgcctacc tcggcaccct cctgagcctg
      421 tggaaggatg acgggctcaa gatcatcgag ctcggccgca gggcgggcct cgagccctcg
      481 accatgacag gcctcatcga ccgcatggaa aaggacggcc tcgtatcgcg cgtgcccgac
      541 cccgaggaca ggcgcgtgat ccgggtgttc ctcacaagtg aaggcaggga catcgagctg
      601 agcgtcgcat cggtggtcga aaaggtgatg gccaagatcc tctccgggtt cgaggacgac
      661 gaggtggcgc tcctcaagga gtcgctcagg aagatgctct cgaacatcca ccaggagaac
      721 gccgatgagt agacctatcc ccgggcacgt gggatacgcg tgctcgtaca cgccgcttgc
      781 cctcgtcctc gcctcgggtt tcaccccata ccgggtgctc cccgtgacgt cgggccccga
      841 ccaggcgggg cttcacctcc acgacaacct gtgcccgcac gtcaagcgca tcctcgacag
      901 ggcgctggca ggcgacctcc cggacatggc cggcatgatc ttcatgaaca gctgcgaccc
      961 catgcgcagg ctcgccgacg catggaccaa ggtcaagggc ggaaacgtcg cgatggtaga
     1021 cctccccgta aacggccttt cgcgctcggt ggacttcttc gcccgggaac tcgggcgcct
     1081 ggcgggcatc ctcgaatcgt ggggcggcga tccggtcacg ccgaagaggt tggcctcggg
     1141 aatcgccgcc acgaacgaga tcgccaatgc gctcgcgggg ctcgagaatc acccgccgtg
     1201 cggcctcggc ggttcggtca tctacgaagc gtacacggcc tgcgccacgt cgccgccggc
     1261 agaggccctg gccttcgtga aagggatcgt ggggagccgt tcgaccagca tggcggatag
     1321 tgacgccccc aaggtcttcg tgttcgggaa cgtattggtc ggcagcgagg caatcaacct
     1381 catagaggcc tgcggcgcca gggtggcgct cgaggacacg tgcatggggt cccgcatgtt
     1441 caggcgcatg gagatggcag agggcgaggt gtcgttcacc gcgctcgcac gtgcgatcct
     1501 ctcgggaaag gcctgcgccc gcacgttcga cccggcccgc ccgggcggga tagccctgga
     1561 tatgctcgag gccgcgaggg gctgcggcgc caggggtgcc atatgccaca cagccaagtt
     1621 ctgcgacccg tacctttcgc gcctgccaca cataagggag gtcttcagaa aggaggggat
     1681 gcccctgctg gtcctcgagg gggactgcac cgcgaggtcc atcggccagc actccaccag
     1741 gatcgaggcc ttcctcgaga tgctgaggtg aaaccatgct gggacagtac ttcgaatcgc
     1801 tcgaatccgg gatcctctcc tccatcgaga aggacggcgg caaggccagc ccgaggaagc
     1861 tctacgcact ggagatcgcg aggctcggca ggaggctgta cgacgggggg cacaaggtcg
     1921 cctggtgcgg ggtcgcggcg cccttcgacc tcttgaacgc catggggctc acctcgtgct
     1981 tcgtggagtt cgtgggcgcc atgctcgcat ccaccggcat ggcggggaac atcatcgagc
     2041 aggcggacca ttggggctac tcctcggacg cctgcagtta ccacaggtcg gtcacggggg
     2101 cggcgttggc aggcctcatg cccgtgcccg agatcctggt gggaacaacc tgcccgtgca
     2161 ccgggggcct cggcgtcatc gagaacctcg ccaggcactt cggcaagccc ctcttcgtgc
     2221 tgaacatccc gcagaacgac acggtggaag acgtccggta cctcgccgac cagctgaggg
     2281 acctcgtggg gttcgcaagc gggcataccg gcatcccctt cgaccaggaa aggctcaccc
     2341 gcgcaatcga aaacaccaac gcagcaaggg agctgttcgt ggagatcttc gacctcgcat
     2401 ccagggtgcc atctcccgcc agcagcaagg acctgaacaa cttcatgatc tcgctcgcgc
     2461 tgctcctggg caccagggag tgcgtggatc tcgcaagggc cttcaaagac gagttcgcgc
     2521 gcagggtaga gacgggctcg gccggtgtga agggggagga gatccggctg ctgtggatcc
     2581 agaaccgcat ccagttcaag aaccccatcg agaagatgct cgaggacgag ttcagggcgt
     2641 gcatcgtcat cgacgagctc aacgacatct actgggaccc catcgaccct gacgacccct
     2701 tcgagggcat cgccaggagg tgcatcctca tccccttcaa cggcccgtcg tccaggcgca
     2761 tcgagcacct caagaaactc gcgctcacat atcgcgtgca cggcgccata aacccctgca
     2821 actgggggtg caggcagggc accggcatga gggggctcat agaggcgggt ctcagggagg
     2881 tgggcgtgcc tgtcctgaac ctggaggtgg actgcgtgga cgatcgaaag ttcaccgaag
     2941 gccagttcag gacgcggata gaggccttca tggagatgat cggttcgaac cggtcttccc
     3001 ggcgcgacag ctgagaggag gggacccgtg tactacctgg gcatagacat aggcagcctg
     3061 tcctgcgacg cggtcctcct cgacgggcac gggcgtgtgg tcgccgacgc catcgtgccg
     3121 acgggggcga agaacgccga ggccatagcg ttggccaccg agctcgtcct gaacagggcg
     3181 ggagccacga gggagaaggt caaggccgtc gtgtccaccg gttacggccg cgacagggtg
     3241 gagggcaggc tggcatcggt aacggagatc acctgccacg caaggggcat gcggcacctg
     3301 aacgagaagg cgcgaatcct catagacatc ggaggccagg acagcaagtg catcctcatg
     3361 tccgagacgg gcgccgtcgt gaacttcgcc atgaacgaca agtgcgccg
//
LOCUS       DAOSCR010000265         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17794_length_3397_cov_3.596349, whole genome shotgun sequence.
ACCESSION   DAOSCR010000265 DAOSCR010000000
VERSION     DAOSCR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17794_length_3397_cov_3.596349"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLT69_07305"
     CDS             complement(<1..702)
                     /locus_tag="PLT69_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ14281.1"
                     /translation="MIDSAKVAWISLWAAIATLVLFLPVTLAALLSSTGNLAFNLSKI
                     WAYVMLAVTNVRPVIRGREKIVPGRSYVIVSNHQSEFDILALVTTLRIQYRWIIKKEL
                     RKVPLFGYALEASRNVFIDRGDHERAMRSIRESMARLPEGVSVLFFAEGTRSEDGSIA
                     PFKKGGFLVALEKGLPILPVTVNGSRRVLPKKSLVFTPGPIEVVVADPIDTTGYSREN
                     LQELVSAAEGFLREAD"
     gene            1150..1974
                     /locus_tag="PLT69_07310"
     CDS             1150..1974
                     /locus_tag="PLT69_07310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ14282.1"
                     /translation="MTTAGLYAGKNVFISGGSSGIGLACAKEFARLGSNVFIFARRRE
                     ALEEAALSIEPHRADPSQRIGFTSVDVTDTEKVADKLRESVASFGPPHVVITCAGIAY
                     PDYFERISASDFELTLRTNLAGTWNVLSVLVPLMHAKGGHVVTVSSIAGFLGVFGYTA
                     YSASKFGVIGLSEALRAELKPKGIRVSVLCPPDTDTPGLAFENRTKPPETRALGKSAG
                     LMSPDAVAKALVSGMARGKFLITPGMEGKLILAAKRLAPSVVMALMDAIVRLSRHG"
     gene            complement(2185..3057)
                     /locus_tag="PLT69_07315"
     CDS             complement(2185..3057)
                     /locus_tag="PLT69_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ14283.1"
                     /translation="MVIDCHYHLEERVFSAERLVREMDARGIHKAALMGSMVSPFKEP
                     PPFLIAVLQRLLESPVTRPIARRLVSDFTDKGEVKILGRPYPIDPDPDNDKVFETVER
                     YPGRFLGWVFVNPRGSRDPVAEYERCKDRPGFVGVKAHPFWHHFRPVELEAVARRLAD
                     AGKPLLIHAGFGEEGDFEALLARVPNLKLILAHAGFPEYRQTWERIKDRPNVYLDVSQ
                     TSYTSRRATFQAAEFLGPGRILFGTDGPYGFHGPDGVYDYGFIKRRIERLFPDKTVRE
                     RILGGNFAEVAGLS"
     gene            3164..>3397
                     /locus_tag="PLT69_07320"
     CDS             3164..>3397
                     /locus_tag="PLT69_07320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ14284.1"
                     /translation="MQGLLALYTASPLEYAACMYRRRSTDGIVGMFADIAGRYDLMNT
                     LMSFGMDKRWRKRVVRLARLFPGAAMLDVGAGTG"
BASE COUNT          595 a         1125 c         1093 g          584 t
ORIGIN      
        1 gtccgcctcg cggaggaacc cctcggctgc gctgaccagt tcctgcaggt tctccctgga
       61 gtaaccggtc gtgtcgatgg gatccgcgac caccacctcg atggggcctg gggtgaaaac
      121 gaggctcttc ttgggcagca ccctcctgct gccgttcacg gtgacgggca ggatgggcag
      181 gcccttctcc agcgccacga ggaaccctcc cttcttgaag ggggcgatgc tcccgtcctc
      241 ggacctggtc ccctcggcga agaagagcac actcacgccc tccggcagtc gcgccatcga
      301 ctccctgatg ctcctcatgg cccgctcgtg gtccccccgg tcgatgaaga cgttgcgtga
      361 ggcctccagc gcgtagccga agagcgggac cttgcgcagt tccttcttga tgatccaccg
      421 gtactggatg cgaagtgtcg tgacgagggc caggatatcg aactcggact ggtggttgct
      481 gacgatcacg tacgacctgc cgggcacgat cttctccctg cccctgatga caggcctcac
      541 gttcgtgacc gcgagcatca cgtacgccca tatcttcgag aggttgaagg cgaggtttcc
      601 cgtcgagctc aagagtgcgg cgagggtcac ggggagaaac agcacgaggg tcgcgatcgc
      661 cgcccacaag gatatccatg caaccttcgc cgagtcgatc aaggatcgac gagttgatcc
      721 catggggccg attatatcaa ccgggatcac gacttcaaca gaaacttcaa gacggtgagg
      781 atcatgtaga aggagaaagg cccgaccagc catgaacggg ccgtgcacct cccgagggag
      841 ggggtcagcc cggtgcggcc cgtgaggccg catcgatggt cgaccggagg cgcgcctcgc
      901 cggcgggttc ccctccccct gcatgccgcc tagcgcctcc cgccgatccc cctgtgcgtt
      961 cacgccgtgg ggcggccccg ggcttcttca aggacggccg tgcctcatgc ccgggcatcc
     1021 accaaggggc tcgtctcccg tcgtccctcg aggccttcga ccgccgtctg cggcctggct
     1081 aaaccacggg gaacgtgtta ccatgcacac cggcggtatg cgaccacagc ccggcatagg
     1141 gaggtgtcca tgacgactgc ggggctctac gctggaaaga acgtgttcat ttccgggggc
     1201 tcgagcggca tcgggctcgc ctgtgcaaag gagttcgcgc gcctgggttc gaacgtcttc
     1261 atcttcgcga ggaggcggga ggcgctcgag gaggccgctc tatcgatcga accccacagg
     1321 gcggacccgt cgcagcggat agggttcacg agcgtggatg tcaccgatac ggagaaggtc
     1381 gcagacaaac tcagggaatc cgtggcatcc ttcgggcctc cgcacgtggt catcacgtgc
     1441 gccggcatcg cgtacccgga ctacttcgag cgcatcagcg catccgactt cgaactgacc
     1501 ctgaggacga acctcgcggg gacctggaac gtcctgagcg tgctcgtccc cctcatgcac
     1561 gccaagggtg ggcacgtggt cacggtgtcg tccatagcgg gcttcctggg ggtgttcggc
     1621 tacacagcct acagcgcgag caagttcggg gtgatcggcc tttccgaggc gcttcgcgcg
     1681 gagctgaagc cgaagggtat ccgggtctcg gtcctgtgcc ctcccgacac ggacaccccg
     1741 ggcctggcgt tcgagaacag gaccaagccg ccagagacca gggcgctcgg caagagcgcc
     1801 ggcctcatga gccccgatgc ggtcgccaag gccctggtat caggcatggc gagggggaag
     1861 ttcctcataa cgcctggcat ggagggcaag ctgatcctcg cagccaagag gctcgcgccg
     1921 tccgtggtca tggccctcat ggacgcgatc gtcaggctct cgagacacgg gtgaccgacc
     1981 tttcggcgac ccctggcggg cgctcgtgac cgccttggtt cgtctcatga ccgcatcacg
     2041 aatgcagccc ttggggtcga ccgccggcgt ttcctcccgc ccccacaccc cgatgctcct
     2101 gggcgggcca tcggcaaaga gacacatcaa gggttgcgcc ccgaaggccg atcgagaggt
     2161 ttcacccctt cggtgcggcc gaggctagga aaggcctgcg acctccgcaa agttcccccc
     2221 gaggatcctc tccctgacgg tcttgtccgg gaacaggcgc tcgatcctgc gcttgatgaa
     2281 cccgtagtcg tacacgccgt cgggcccgtg gaaaccgtaa gggccgtcgg tcccgaacag
     2341 gatcctcccg gggcccagaa actccgcggc ctgaaacgtc gcgcgcctgc tcgtgtaact
     2401 cgtctgcgag acgtcgagat acacattggg cctgtccttg atcctctccc acgtctgccg
     2461 gtactcgggg aaaccggcgt gtgcgaggat cagcttgagg ttcggcaccc gagcgaggag
     2521 ggcctcgaag tcgccctcct cgccgaaacc ggcatggatc aggaggggtt tgcctgcatc
     2581 ggcaaggcgc ctcgccacgg cctcgagctc caccggcctg aagtggtgcc agaacgggtg
     2641 ggccttgaca cccacgaacc cgggacgatc cttgcatcgt tcgtactccg cgaccgggtc
     2701 cctcgaaccc ctgggattca cgaacaccca cccgaggaac ctccccgggt accgctcgac
     2761 cgtctcgaac accttgtcgt tgtcgggatc agggtcgatg gggtagggcc ttccgaggat
     2821 cttgacctcg cccttgtcgg tgaagtccga gacgagcctg cgcgcgatag gcctcgtcac
     2881 cgggctctcg agcaggcgct gcagcaccgc gatgagaaag ggcggcggtt ccttgaacgg
     2941 actcaccatg ctccccatga gcgccgcctt gtgtatgccg cgtgcgtcca tctccctgac
     3001 gagcctctcc gcagagaaca ccctctcctc cagatggtag tgacaatcga tgaccatgca
     3061 tccccccgct gggcctcgtg taccggcgcc ggggccgctc gagccgggcg ccggccggcc
     3121 gtgtgatgta tcgcctcaag aaaagtagct gccgcccggg ggaatgcaag ggctgcttgc
     3181 cctttacacg gcgagcccgc tcgagtatgc agcgtgcatg taccggcgga gatcgacaga
     3241 cggcatcgtg ggcatgttcg cagacatcgc agggcgatac gacctcatga acaccctcat
     3301 gagcttcggg atggacaaga ggtggaggaa acgcgtggtc aggctcgcga ggctcttccc
     3361 cggggcggcc atgctggacg tgggggcggg cacgggc
//
LOCUS       DAOSCR010000266         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17797_length_3397_cov_2.651107, whole genome shotgun sequence.
ACCESSION   DAOSCR010000266 DAOSCR010000000
VERSION     DAOSCR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17797_length_3397_cov_2.651107"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="tgt"
                     /locus_tag="PLT69_07325"
     CDS             <1..867
                     /gene="tgt"
                     /locus_tag="PLT69_07325"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ14285.1"
                     /translation="HAFMGWDGPILTDSGGFQVFSLSPLVRVTSDGVMFASHLDGSRR
                     MLTPEKAIEIQEALGSDIMMCLDECVAYPSPLPKVEQACDRTVRWAARSLEAKKTDAA
                     LFGIVQGGMDDGLRKRCASALAGLPFDGLAIGGLSVGEGHDVMMAVLGRTTAHLPQDK
                     PRYLMGVGTPVDIVEAVGMGVDLFDCVLPTRNARNGTLFTSAGRLSLKQARFREDPGP
                     PDASCSCYTCTHFSRAYLRHLHVSGEILGSVLNTIHNLHFYLELMSGIRHSIEERRFE
                     DLKTKIKEMFDD"
     gene            860..1180
                     /gene="yajC"
                     /locus_tag="PLT69_07330"
     CDS             860..1180
                     /gene="yajC"
                     /locus_tag="PLT69_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOJ14286.1"
                     /translation="MTSIAYAATPQGGDLMSVLVSVAPLVLIFVVFYFLLILPQKKKA
                     QEHKKMLESIQKGDDVITSGGIHGKVVGVADNVITLDVGDKVKIKVSREYIAQKKGAE
                     AAQK"
     gene            1198..2772
                     /gene="secD"
                     /locus_tag="PLT69_07335"
     CDS             1198..2772
                     /gene="secD"
                     /locus_tag="PLT69_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOJ14287.1"
                     /translation="MDRFKVRGILILAVLVAAVVLALPSFLGKTELPQGWIGPKSKLR
                     LGLDLQGGIHMVLKVDSLKAVENRLNTLAGDLKPHMIENRIKYTSVAFTAPDKITVDL
                     RGADDYDALSKLMADSYPNLVEVDRKDEPELTRVVYQLKDAEVKAIRSQAVDQALETI
                     RNRIDQFGVTEPSIVPQSGEKIVLQLPGVTDPERAKRLIGRTAVLEFKLVDEEHSVEE
                     ALQGKVPEGSYVAYMRDGKQPILLKETAVLTGSMLNDAQGRISPSNNMPYVAISFNPE
                     GARVFERVTSENVGKRLAIVLDGKVYSAPVIRDAIAGGKAVIEGRFTDQEARDLALVL
                     RSGALPAPVDIIEERTVGPSLGEDSIRMGVYASLVGAALVVIFMVVYYKVSGLIANVA
                     LAANIVMLVAVMALFHATLTLPGIAGIGLTIGMAVDANILVFERIREEIRLGRTPVMA
                     LDAGYKNALWTILDANITTLIAAVVLFQFGTGPIKGFAVTLSVGILTTLFTTLIMSKW
                     IQEWLVNSLKVEEISI"
     gene            2783..>3397
                     /gene="secF"
                     /locus_tag="PLT69_07340"
     CDS             2783..>3397
                     /gene="secF"
                     /locus_tag="PLT69_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOJ14288.1"
                     /translation="MELIRPGIFIDFIAYRKYAYLVSGVLIALSIVSIFLVKGLNYGI
                     DFSGGLDLQVAFKERVKTEDVRAAMRKAGLEGASIQSISGGGRENEYLVRMQKISEDP
                     KQSVTTVAEDALFKAFGKDKVDVRRAEVVGAAVSKDLKTKGLLSIVYACIGLLVYIWV
                     RFEFSYSLGAIAALIHDVTITLGVFSLLGKEISLTIIAALLTIIG"
BASE COUNT          671 a         1052 c         1098 g          576 t
ORIGIN      
        1 catgccttca tggggtggga cggccccatc ctcaccgaca gcggggggtt ccaggtgttc
       61 agcctgtccc cgctcgtgag ggtcacgagc gacggcgtga tgttcgcgtc ccacctcgac
      121 ggcagccgtc gcatgctgac ccccgagaag gcgatcgaga tccaggaggc gctcggctcc
      181 gacatcatga tgtgcctgga cgagtgcgtg gcgtacccga gcccgttgcc caaggtggag
      241 caggcgtgcg acaggaccgt ccggtgggcg gccaggagcc tggaggcgaa gaagacggac
      301 gccgccctgt tcgggatcgt gcagggcggc atggacgacg gcctgaggaa aaggtgtgca
      361 tccgcgctgg ccgggctccc cttcgacggg ctcgctatcg gcggcctctc ggtcggcgag
      421 gggcacgacg tcatgatggc ggtgctcggc cgcacgaccg cccacctgcc ccaggacaag
      481 ccccgttacc tcatgggcgt gggcacccct gtggatatcg tggaggcggt gggcatgggc
      541 gtcgacctgt tcgactgcgt cctcccgacg cgcaacgccc gcaacgggac cctcttcacc
      601 agcgcgggga gactgtctct caagcaggcc cgcttcaggg aggaccccgg gcctcccgac
      661 gcatcctgct cgtgctacac gtgcacacac ttcagccggg cctacctcag gcacctccac
      721 gtgtccgggg agatcctcgg gtccgtcctc aacaccatcc acaacctgca cttctatctt
      781 gagctgatgt ctggtatacg gcattccatt gaggagcggc gcttcgaaga cctcaagacc
      841 aagatcaagg agatgttcga tgactagtat cgcatacgca gcgacaccgc agggaggaga
      901 cctcatgagc gtcctggtga gcgtcgctcc cctggtgctc atcttcgtcg tgttctactt
      961 cctgctcatc ctgccccaga agaagaaggc gcaggaacac aagaagatgc tggaaagcat
     1021 ccagaagggc gacgacgtga tcacgagcgg ggggatccac ggaaaggtcg tgggtgtggc
     1081 ggacaacgtg atcactctcg atgtcggaga caaggtcaag atcaaggtct cgagggagta
     1141 catcgcacag aagaagggcg ccgaggccgc tcaaaaataa cattcggggg ttttgcaatg
     1201 gataggttca aggtgagggg catcctcatc ctcgcggtgc tggtggcggc cgtcgtcctg
     1261 gcgctgccga gtttcctggg aaagaccgag ctgccccagg gctggatcgg ccccaaatcc
     1321 aagctgagac tggggctcga cctccagggc ggcatacaca tggtcctgaa ggtggattcg
     1381 ctcaaggctg tggagaacag gctgaacacg cttgcgggcg atctcaagcc ccatatgatc
     1441 gaaaaccgga tcaagtacac gtcggtggcg ttcaccgcgc cggacaagat caccgtcgac
     1501 ctgagggggg cggatgacta cgatgcgctc tcgaagctca tggccgattc ctacccgaac
     1561 ctcgtcgagg tcgaccggaa ggacgagccg gagctcacgc gggtcgtcta ccagctcaag
     1621 gacgcggagg tgaaggccat caggagccag gccgtcgacc aggcgctcga gacgatccgc
     1681 aaccgcatcg accagttcgg cgtgaccgag ccctccatcg tgccccaatc gggcgagaag
     1741 atcgtgctcc agctgccggg cgtcacagac cccgagaggg ccaagaggct catcggacgc
     1801 accgccgtgc tcgagttcaa gctcgtcgac gaggaacact cggtcgagga agcgctccag
     1861 gggaaggtgc ccgagggcag ctacgtcgcc tacatgcgcg acgggaagca acccatcctc
     1921 ctgaaggaga cggcggtgct cacgggcagc atgctgaacg acgcccaggg caggatcagc
     1981 ccctcgaaca acatgcccta cgtggccatt tccttcaacc ctgaaggggc gcgcgtcttc
     2041 gagcgcgtca cgagcgagaa cgtcggcaag cgcctggcca tagtgctcga cggcaaggtc
     2101 tactcggctc ccgtcatcag ggatgcgatc gccggcggga aggccgtcat agagggacgg
     2161 ttcaccgacc aggaggcgcg cgacctcgcc ctcgtgctca ggtccggcgc cctgcccgca
     2221 ccggtggaca tcatcgagga aaggaccgtc gggccctcgc tcggggaaga ctcgatccgg
     2281 atgggggtct acgcctcgct cgtgggcgcg gccctcgtcg tgatcttcat ggttgtctac
     2341 tacaaggtct ccgggctgat cgcgaacgtg gccttggctg cgaacatcgt catgctcgtg
     2401 gcggtcatgg ccctgttcca cgcgaccctc accctgccgg gcatcgccgg gatcgggctc
     2461 accataggca tggccgtgga cgcgaacatc ctcgtgttcg agcgcataag ggaggagata
     2521 cgcctcggaa ggacccccgt gatggccctg gatgcgggtt acaagaacgc cctgtggacc
     2581 atcctcgacg cgaacatcac caccctgatc gcggcggtgg tgctcttcca gttcgggaca
     2641 ggccccatca agggcttcgc cgtgaccctg agcgtgggca tcctgacaac gctcttcacc
     2701 acactcatca tgtccaagtg gatacaggag tggctggtga acagtctcaa ggttgaagag
     2761 ataagcatct gagggggccg acatggaact gatacgaccc ggcattttca tcgacttcat
     2821 agcataccgc aagtacgcct acctggtgtc aggcgtgctc atagcgctga gcatcgtgag
     2881 catcttcctc gtcaagggcc tcaactacgg catcgacttc tcgggcggtc tcgatctcca
     2941 ggtggccttc aaggaaaggg tcaagaccga ggacgtccgt gcggcaatga ggaaggccgg
     3001 cctcgagggg gcgagcatcc agagcataag cggcgggggc agggagaacg agtacctcgt
     3061 caggatgcag aagatctcgg aagacccgaa gcagagcgtc acgacggtcg ccgaggacgc
     3121 cctgttcaag gccttcggga aagacaaggt cgacgtgagg cgcgccgagg tggtgggcgc
     3181 ggcagtgagc aaggacctca agaccaaggg gctgctctcg atcgtgtacg cgtgcatagg
     3241 cctgctcgtc tacatctggg tgcgtttcga gttctcgtat tcccttgggg ccatcgcggc
     3301 gctcatccac gacgttacca taaccttggg cgtcttctcc ctgctcggca aggagatcag
     3361 cctgaccatc atcgccgcgc tcctgaccat catcggc
//
LOCUS       DAOSCR010000267         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17803_length_3396_cov_4.239449, whole genome shotgun sequence.
ACCESSION   DAOSCR010000267 DAOSCR010000000
VERSION     DAOSCR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17803_length_3396_cov_4.239449"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="PLT69_07345"
     CDS             complement(<1..90)
                     /locus_tag="PLT69_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOJ14289.1"
                     /translation="MQPYFFPYIGYFQLINAVDFYHKMRKILGS"
     gene            complement(105..1229)
                     /locus_tag="PLT69_07350"
     CDS             complement(105..1229)
                     /locus_tag="PLT69_07350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ14290.1"
                     /translation="MSKETKTTYVTQPALPPLEEFVESLKTIWESRILTNKGPFHKRF
                     EKELADYLGVKFISLTANGTLALVTALQALRITGEVITTPYSFVATTHSLWWNNIKPV
                     FVDIDPETCNLDPGRIEAAITPHTTAILPVHVYGNPCDVNQIQEIAGIYGLKVIYDAC
                     HTFGVTMDGVPVLNFGDLSVMSFHATKTFTTFEGGAIVCHDEAMKKRIDFLINFGFAG
                     ETTVVAPGINSKMNEVQAAMGLLQLRHIDGYLARRRQIVSLYRERLTKVPGLRMMQDM
                     PGVKHCYTYFPVFVNEGEYGMSRDALYEKLKQNGIYCRRYFYPLISQFPMYRGLESAQ
                     PGRLPAAEKATGEVLCLPVYPDLTDATVEHIVELLQRDRT"
     gene            complement(2182..>3396)
                     /locus_tag="PLT69_07355"
     CDS             complement(2182..>3396)
                     /locus_tag="PLT69_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOJ14291.1"
                     /translation="RINTPIALSPSPPKTTRTPPGKRVAVTGAGGSIGSELCRQILQY
                     NPSSLILIDNAEPPLFHIDMELRGMLRAGSEVRIVPCIGDVRSRHSIERIFRSHSPQL
                     VYHAAAYKHVPMMELAPIDTVNNNVIGTFKVASLACKYHVEDFVLISTDKAVKPTSVM
                     GATKRVAELVVQALSGNDTRFIVVRFGNVLGSNGSVVPLFQQQIAAGGPVTVTHPDVT
                     RSFMTIHEAVMLVLQAGAIGNGGELFLLDMGRPVKILDMARNMIRLAGLVPDRDVKIS
                     FIGLRPGEKSTKSFSSTVKASWIPRTRKSRSVAPAVRSMRRASLEGIERMNLLVKNSG
                     DAEAALAILEALVPDFRRETGYRQAAETAQSTRHRHDRDRLVVLSRNPSYRFVSAPRK
                     RKFTAVQEFSDS"
BASE COUNT          627 a          996 c         1015 g          758 t
ORIGIN      
        1 gctgcccagg atcttgcgca tcttgtggta gaagtccacg gcgttgatca gctggaaata
       61 cccaatgtaa gggaaaaagt aaggctgcat tatggcgagt ttcttcacgt gcggtctctc
      121 tgcaacagct ccacgatgtg ttctacggtc gcatcggtca ggtcgggata gaccggcagg
      181 cagagcacct ctcccgtggc cttttccgct gccggtagtc tgcccggttg cgcggattcc
      241 agcccccggt acatggggaa ctggctgatg agggggtaga aataacggcg gcagtagatg
      301 ccgttctgct tgagtttttc gtagagggcg tcgcggctca tgccgtattc cccctcgttc
      361 acgaaaacag ggaaatacgt atagcagtgt ttcaccccgg gcatgtcttg catcatgcga
      421 aggccgggca ccttggtgag ccgctcgcga tacaaggaca caatctgtcg gcgcctggcc
      481 aagtacccgt cgatatgcct gagttgcagc aggcccatgg cggcctgcac ctcgttcatc
      541 ttggagttga tgccgggagc cacgacggtg gtctcgccgg caaacccgaa gttaatgagg
      601 aaatcgatgc gcttcttcat ggcctcgtcg tggcacacga tggccccgcc ctcgaaggtg
      661 gtgaaggtct tggtggcatg aaagctcatg actgagaggt ccccgaagtt caagacaggc
      721 acgccatcca tggtgacgcc gaaagtgtgg caggcgtcgt agatcacctt caagccgtag
      781 attcccgcga tttcttgtat ctggttcacg tcgcaggggt tgccgtacac gtgaacgggg
      841 aggatggcgg tggtatgcgg ggtgatggcg gcctcgatcc tgccggggtc gaggttacag
      901 gtttcagggt cgatgtccac gaacacgggc ttgatgttgt tccaccacag ggaatgggtg
      961 gtggccacga agctgtacgg ggtggtgatg acctcgccgg ttatacgcag ggcctgcagc
     1021 gccgtcacca gggccagggt tccgttggcc gtgagcgaga tgaacttcac gcccagatag
     1081 tctgccagct ccttttcgaa cctcttgtga aagggcccct tgttggtgag gatgcggctt
     1141 tcccagatgg tcttcaagga ttccacgaac tcctccagcg gcggcagggc aggctgggtc
     1201 acatacgtgg tcttcgtttc cttgctcatg ggtcatcctt gtcttaactg tacacagtgt
     1261 tgcttctgca tgtgttcgat tccgatcgga ccagctgtca tgaggtcgtt tttcattcaa
     1321 gtgcaccaaa gggggccgta tgagcagcct gattccccca cctgacggtc tgtcctcttc
     1381 tttaccatac ttcgggcacc catgtccctc gttcactggc accagagccg gaaaccttgc
     1441 cgcctcccgg tacgacttcc cgatatggtg agggcccagg cctgacctgg ccggtcccac
     1501 ccctctttcg ccagggtggt gaggcgggct cgccccaccc cttggcgcag cgccgtgaca
     1561 ccaacatcct gcacgatctt gagcctgctc atcggtgtgg aagcagaccg cacaagccgg
     1621 catccacccc tgcccggcat ctctgggaaa ccccctctcc tgcagccggc ccagaacctc
     1681 ggcatggacc cgctccatca ccctttcccc ccacggtctc gggagcggct gatcacgcgg
     1741 tgaccgagag agcgttcccc aagctgcagc ccgatgtaca ccttgaacaa acccggtcat
     1801 cggactggtt ttccatttca ggggcgctcc agggccaaca ccaccattgc ttcactatcg
     1861 cctgtagcaa cacgaacgta tgattggccg cttcggaggt tcgtacctga tgtggtcatg
     1921 agtctccttg agtacggcct tccacctcga ggttcccagg gttttgctcg gcagcatgtc
     1981 cagaaggctc atggtcttct cgcattcctt gagccttata gcgtctcccc caactcattc
     2041 caccttggga caagaccacc atatatgccc catggtatct tttggtaaat cgccttcatc
     2101 atcgtgggaa tggagcgcct gtcacatctt ctcttcaggt gggttgtgaa aaaaggaaag
     2161 tgtcccctcc gcaggtgctc gtcaagagtc ggaaaactct tgtacggccg tgaacttcct
     2221 ctttcgtggc gccgatacga accggtaaga ggggttccgg cttagtacaa cgagccgatc
     2281 gcgatcgtga cgatgccggg tgctttgtgc ggtctcagca gcctgcctat agccggtctc
     2341 tctcctgaag tcgggcacaa gcgcttcgag gatggcgagt gcagcctccg catcgccgga
     2401 gtttttcacg agcagattca tgcgttcgat gccttctaaa gaggctcgcc tcatcgatct
     2461 tacggcaggt gctacagacc ttgattttct ggtacgcggt atccacgatg ccttcaccgt
     2521 cgatgagaag ctcttcgtgg atttttcccc gggccgtagg ccgatgaagg agatcttcac
     2581 gtcgcggtcc ggcacaaggc cagccaggcg gatcatgttc ctggccatgt ccaagatctt
     2641 cacgggcctg cccatgtcga gcaagaacag ctcaccgccg ttgccgatcg ccccggcctg
     2701 gaggaccagc atgaccgcct cgtggatggt catgaaggag cgggtgacat ctgggtgggt
     2761 cacggtcacc ggcccgccgg cggcgatctg ctgttgaaag aggggtacca cgctgccgtt
     2821 gctgcccagg acattgccga accgtaccac gatgaaccgc gtgtcgttgc cgctgagcgc
     2881 ctggacgacg agttctgcca cccgcttcgt ggcacccatg acagaggtgg gtttcaccgc
     2941 cttgtctgtt gagatgagga caaaatcctc cacgtggtac ttgcaggcca gggatgcgac
     3001 cttgaaggtg ccgatgacgt tgttgttcac ggtatcgatg ggggcgagct ccatcatggg
     3061 tacgtgcttg tatgctgccg cgtgatacac gagctgaggc gagtgactcc tgaagatgcg
     3121 ctctatgcta tgcctgctcc gcacatcgcc gatgcacggc acgatccgga cctccgaacc
     3181 ggcccgcagc atccctctga gctccatgtc gatgtggaag agggggggtt ccgcgttgtc
     3241 gatcaggatg agggatgagg ggttgtattg aaggatctgc ctacagagct cagacccaat
     3301 ggacccgccc gcgcccgtta ccgccaccct cttccctgga ggcgtgcggg tcgttttcgg
     3361 gggggagggg gagagggcga tgggggtgtt gatcct
//
LOCUS       DAOSCR010000268         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17818_length_3394_cov_3.365678, whole genome shotgun sequence.
ACCESSION   DAOSCR010000268 DAOSCR010000000
VERSION     DAOSCR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17818_length_3394_cov_3.365678"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..2089)
                     /gene="tkt"
                     /locus_tag="PLT69_07360"
     CDS             complement(20..2089)
                     /gene="tkt"
                     /locus_tag="PLT69_07360"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ14292.1"
                     /translation="MNTTQDIHARAIDTIRFLAADMIQQAESGHPGLPLGAAPMAYVL
                     WSKYLKHNPANPRWVDRDRFVLSAGHGSALLYALLHLTGYDLPMEELKRFRQWKSRTP
                     GHPETTLTPGVEMTTGPLGQGIASAVGMALAEAHLASVFNRPGHEIVDHYTYVLAADG
                     DLMEGVSYEACSLAGHLRLGKLIVLYDSNRISLAGSTELCFTEDVEKRFEACGWHTQT
                     VVDGNDLAAIDIALMAAREERSMPSLIIVRTTIGFGAPTKQGSHKVHGSPLGVDELRR
                     AKENRGFDPQKTFHVPEDVRRHLGSALETGKRSESEWQQAFFRYNRSYPDLADQFKMR
                     MAGELPRGWDEGMAELSTGTKPVSTRKASEAVLQVLSKNIPALVGGSADLNPSTLTWM
                     KECGDFQAATACPEGTQGAVGSCWGREGRNIHYGVREHAMAAMSTGMSLHGGIIPYCS
                     TFLTFSDYMKPAIRLACLCHARVVYVFTHDSIGVGEDGPTHQPVEHLMGLRGTPNLAV
                     IRPADAAETAYAWKVALGRTEGPTALILTRQDVPAIDRSVCASARGLESGGYILWESG
                     SKILELILIATGSELGIALDAARELARRKLSVRVVSMPCWEIFDAQCKEYRDKVLPPE
                     VRARVAVEAGLRLGWEHYVGPEGAVVGLDGFGASAPASVLFKEFGVTLDAVVDVSLGL
                     VKKRKKR"
     gene            complement(2108..3109)
                     /gene="glk"
                     /locus_tag="PLT69_07365"
     CDS             complement(2108..3109)
                     /gene="glk"
                     /locus_tag="PLT69_07365"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOJ14293.1"
                     /translation="MSLLLAGDVGGTKTTLACYRAGRDHLELLRRASFPSRSFSCLKE
                     VLDAFMEGFDAPLDAAVLGVAGPVVDGTATITNLPWKVDERELAVILKTARTSVVNDL
                     VAVSYGILDLSAGDLFTLNDVPRDPRGPIAVIAPGTGLGEAYLTWDGASYRAHPSEGG
                     HADFAPTDETQISLLRWMLRRHDHVSVERVCSGSGIPNLYAFLKEQGAHEEPERLALR
                     LGEAPDPAVVIRENAQDPVEGCDLSRAAMELFVKILGAEAGNLALKLLPTGGLFLAGG
                     LPPRIIPMLETGAFMEAFTSKGRLSAWVKRIPVLVALDLDVGLKGAARYGKTMLLER"
BASE COUNT          528 a         1117 c         1152 g          597 t
ORIGIN      
        1 catggcgcca cccccgttgt caccttttct tccgtttctt gaccagaccc agggaaacat
       61 ccacgacggc gtcgagggtg acgccgaact ccttgaagag gacgcttgcc ggcgcgctcg
      121 ccccgaagcc gtcgaggccg accaccgcgc cctcgggccc cacgtagtgc tcccacccga
      181 gcctcagccc ggcctcgacc gcgaccctgg ccctgacctc gggaggcagc accttgtccc
      241 ggtattcctt gcactgggcg tcgaagatct cccagcacgg catggacacc accctgacgg
      301 acagcttacg cctggcgagc tctcgtgcgg cgtccagcgc tatgcccagc tccgagccgg
      361 tggctatgag gatgagttcg aggatcttgg aaccggactc ccacaggatg taaccgccgc
      421 tctcgagccc cctggcgctc gcgcagacgg accggtcgat ggcgggcacg tcctggcggg
      481 tcaaaatcaa cgcggtcggt ccctcggtgc gcccgagcgc caccttccat gcgtaggccg
      541 tctcggcggc gtcagcgggc ctgatgacgg cgaggttcgg tgtgcccctg agccccatga
      601 ggtgttcgac aggctggtgc gtcggcccgt cttcaccgac ccctatgctg tcgtgggtga
      661 acacgtacac cacccgggcg tgacacaggc aggcgagcct gatggccggt ttcatgtaat
      721 cggagaaggt gaggaacgtg gagcagtagg ggatgatccc cccgtgcagg gacatgcccg
      781 tggacatcgc ggccatggcg tgctcgcgca caccgtaatg gatgttcctc ccctcgcgcc
      841 cccagcacga gccgaccgct ccctgcgtgc cctcagggca ggcggtggct gcctggaagt
      901 ctccacactc tttcatccac gtcagggtgg atgggttgag gtcggcggac cccccgacga
      961 gggcagggat gtttttcgac aggacctgga gcaccgcctc ggacgccttc ctcgtggaga
     1021 cgggcttcgt acccgtggac agctcggcca tgccctcgtc ccatccccgg gggagctcgc
     1081 ctgccatgcg catcttgaac tggtccgcaa ggtccgggta ggacctgttg tacctgaaga
     1141 aggcctgttg ccattccgac tcggagcgtt tcccggtttc cagggccgag ccgaggtgtc
     1201 ttctcacgtc ttcgggcacg tggaaggtct tttgcgggtc gaagccgcgg ttttccttgg
     1261 cgcgccttag ctcgtcgacg ccgaggggcg agccgtggac cttgtgggaa ccctgcttgg
     1321 tcggcgcgcc gaagccgatg gtcgtgcgga cgatgatgag ggagggcatg gaacgttcct
     1381 cgcgtgcggc catgagggcg atgtcgatgg cggcaaggtc gttcccgtcc acgacggtct
     1441 gggtgtgcca gccgcaggcc tcgaaccgct tctccacgtc ctcggtgaag cacagctcgg
     1501 tggagcccgc cagcgagatc ctgttgctgt cgtagaggac gatgagcttg cccaggcgca
     1561 ggtgacccgc cagcgagcag gcctcgtagg agaccccctc catgaggtcg ccgtccgcgg
     1621 ccaggacata ggtgtagtgg tccacgatct cgtgcccggg ccggttgaac acggaggcga
     1681 ggtgagcctc cgccagcgcc atgcccacgg cgctcgcgat gccctgtccc agggggccgg
     1741 tggtcatctc gacgcccggg gtgagggtgg tctcagggtg tccgggtgtc ctgctcttcc
     1801 actggcggaa acgtttcagc tcctccatgg gaaggtcgta gccggtcagg tgaaggagcg
     1861 catagagcag tgccgagccg tggcctgcgg agagcacgaa gcggtccctg tccacccacc
     1921 tcgggttcgc cgggttgtgc ttgagatact tgctccacag gacgtacgcc atgggagccg
     1981 ccccgagagg cagccccggg tgtcccgact cggcctgctg tatcatgtct gcggccagga
     2041 aacggatggt gtcgattgca cgggcgtgaa tgtcttgggt ggtgttcact cgggaatacc
     2101 tccggtttca acgttcgagg agcatggtct tgccgtagcg cgccgcaccc ttcaggccta
     2161 cgtccaggtc gagcgccacg agcaccggta tgcgtttcac ccaggcgctc aggcggccct
     2221 tcgaggtgaa cgcctccatg aaggcgccgg tctcgagcat ggggatgatc ctgggcggca
     2281 accccccggc gaggaacagc cccccggtgg gcaggagctt gagcgcgagg ttgccggcct
     2341 ctgccccgag gatcttcacg aacagctcca tggccgccct cgacaggtcg caaccctcga
     2401 cagggtcctg cgcgttctcg cggatgacca cggccgggtc gggggcttcc ccgagccgga
     2461 gcgccagccg ctcgggctcc tcgtgcgcgc cctgctcctt caggaaggcg tagaggttgg
     2521 ggatgccgct cccggagcag acgcgctcca cgctcacgtg atcgtggcgg cggagcatcc
     2581 acctcaggag cgatatctgg gtttcgtccg taggcgcgaa gtccgcgtgc ccgccctctg
     2641 aagggtgggc cctgtaggac gcgccgtccc acgtcaggta ggcctcgccc agccctgtgc
     2701 ccggcgcgat caccgcgatg ggccccctcg ggtccctggg gacgtcgttc aaggtgaaca
     2761 ggtctcctgc ggaaagatcg aggatgccgt aggaaacggc gacgaggtcg ttgaccacgg
     2821 aggtgcgggc ggtcttgagg atgaccgcga gctcgcgttc atcgaccttc catgggaggt
     2881 tcgtgatggt cgcggtccca tccacgacgg gacccgccac cccgagcacg gcggcgtcga
     2941 gcggggcgtc gaaaccctcc atgaatgcgt cgagcacctc tttcaggcag ctgaacgagc
     3001 gtgaggggaa ggaggccctc ctgagcagtt ccaggtggtc tcgaccggca cggtagcatg
     3061 cgagcgtggt cttcgtgccg cccacgtccc ccgctaggag caggctcatg acaaggcccc
     3121 ccggggcctg gccgatgtga gtcgccggtt catggctctg tctaatacac gaaacccggt
     3181 ggtgtctcaa ggttcaggca aaacgtctcg cctgcggcaa ggccggccgg gcacgacggc
     3241 tgcccgaggg tctccaggca gggaaggatg atcgtctctg ccttctgcgt gtgtcctgga
     3301 cggccgcggg gccatcgccg gacgcccgct cgagtcgaag acgcgtaagt tttcccaggg
     3361 tgcgccgaag cgcactgtcc tccgggtacg ggcc
//
LOCUS       DAOSCR010000269         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17837_length_3392_cov_3.551393, whole genome shotgun sequence.
ACCESSION   DAOSCR010000269 DAOSCR010000000
VERSION     DAOSCR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17837_length_3392_cov_3.551393"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1969
                     /locus_tag="PLT69_07370"
     CDS             <1..1969
                     /locus_tag="PLT69_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOJ14294.1"
                     /translation="YAFVDVDGMDTGVYVPRSGLGGALPGDLVEVQAWPGRLGLRGRV
                     TSVVERESIVVTGRYTSRVGFGVLEPHQAFPYTIIIPEGAHGSARSGDTVSALVDPPR
                     RPGRVDAVTARVLKPIRFPEGIGEDLRLVSSKYALSWAFPREVEREAREVSLIDMGRE
                     LSRRRDLRERILFTIDPPDARDHDDALGIEEMGDGTLLLTVAIADVAHVVKRGGALDR
                     EARKRGFSVYFPGACIPMLPEVLSSDAMSLSPGQDRLAVCVELVFAENAEPVSCRIFE
                     AVVRSRARLSYDEVGPFLEGARGGDERFGQEVASRLTALDRICRRIREKRLALGGLDL
                     EIADIAVGLAPSGCVSTLERASQNAAHRLVEEAMLLANRAVCRFLTEKKRHALFRVHE
                     QPGRKDLMDLAEVLAEIPGMLGPANLVMRGASSGSRVREALQAALDAARSTPLESFVA
                     RQVVKALKRARYSPDDIGHFGLAFDGYLHFTSPIRRYPDLVVHRILKGVLRGEGGRPE
                     RTDRKTLERLAQEMSMREELTDNAMREALRLKAASFMAQRMGEDFDAVVTALRPGGLF
                     VEVLDIPVEGVVRGDSFDGGGRAVRRRRARPGLGVGDVARVRLVRADTTSGQLDFVLV
                     PPEGRDAAEAGIDARPRRSARGRPGRGRKDR"
     gene            2024..2098
                     /locus_tag="PLT69_07375"
     tRNA            2024..2098
                     /locus_tag="PLT69_07375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2056..2058,aa:Gly,seq:gcc)
     gene            complement(2205..>3392)
                     /gene="asnS"
                     /locus_tag="PLT69_07380"
     CDS             complement(2205..>3392)
                     /gene="asnS"
                     /locus_tag="PLT69_07380"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOJ14295.1"
                     /translation="IIRDGSGFCQAVVEREALGEDYEEVRKLPLESSVELTGTVVAEP
                     RSEGGVELHASGIKIFQKSEEFPIGKKEHGPDFLLGLRHLWIRSPRQVAILRIRDALV
                     RHVRNFFHENGFTLIDTPIFTTTVGEDSSNLFSVEYFDLGRTYLSQTGQLYLEAAIFA
                     LGKVFCLGPTFRAERSKTRRHLTEFWMVEAEAAFSRHQDNLELQENFVSYIVQNTVRE
                     CSPELAKLQRDTEPLKKIEPPFHRLSYDEAVEVLRKNGSDIVWGDDLGGDDETILTQL
                     YDKPVFIECYPRKAKAFYMKQHPERPELVLCADLLAPEGYGEIIGGSEREDDPEILMK
                     AILDNGLDPERYGWYFDLRKYGSVPHSGFGLGIERTLAWICGLKHVRETIPFPRMLYR
                     AYP"
BASE COUNT          561 a         1031 c         1204 g          596 t
ORIGIN      
        1 atacgccttc gtcgacgtgg acggaatgga caccggcgtc tacgtgcccc gttcgggact
       61 gggcggcgcc ctgccggggg atctcgtgga ggtccaggcc tggcctggga ggctgggtct
      121 tcggggcagg gtgacctcgg tggtggaacg tgaatcgatc gtggtgaccg ggcgttacac
      181 gagccgggtg gggttcggcg tgctcgagcc gcaccaggcg ttcccctaca cgatcatcat
      241 ccctgagggc gcccacggga gcgcaagaag cggcgacacg gtgagcgccc tcgtggatcc
      301 gccccggcgc cctggccgcg tggacgcagt gacggcgagg gtgctgaagc ccatacggtt
      361 tcccgaaggc ataggggagg acctcaggct cgtctcttcg aagtacgccc tgtcgtgggc
      421 tttcccccgg gaggtggagc gcgaggcgcg ggaggtctcc ctgatcgaca tgggccgtga
      481 gctctcccgc aggagagacc tgagggagcg catcctgttc accatagacc ccccggatgc
      541 gagggaccac gacgacgccc tgggcatcga ggaaatgggg gacgggaccc tgctcttgac
      601 cgtcgcgatc gcggatgtcg cgcatgtggt gaagagaggg ggcgccctgg acagggaggc
      661 gaggaagcgg ggattcagcg tgtatttccc gggggcgtgc atacccatgc tccccgaggt
      721 gctcagctcc gacgccatga gcctgagccc cggccaggac aggctcgccg tgtgcgtcga
      781 gctcgtcttc gccgagaacg cggagcccgt ctcgtgccgg atattcgagg cggtggtgcg
      841 ctcgagggcg aggctctcct acgacgaggt gggccctttc ctcgagggtg cacggggagg
      901 tgacgaacgg ttcggccagg aggtcgcctc gaggctgaca gccctggacc gcatctgcag
      961 gaggatcagg gagaagaggc tcgctctcgg ggggctcgac ctcgagatcg ccgacatcgc
     1021 cgtcggcctg gcgccttcag ggtgcgtctc gacgctggag agggcgtcgc agaatgcggc
     1081 ccacaggctc gtggaagagg ccatgctcct tgcgaacagg gcggtctgca ggtttttaac
     1141 ggaaaagaag aggcacgccc tcttcagggt acacgagcag ccgggacgca aggatctcat
     1201 ggatctcgcg gaggtgctgg cggagatccc cggcatgctg ggccccgcaa acctcgtcat
     1261 gcgcggtgcc tcttccggat cgagggtacg ggaggccctc caggcggcct tggacgccgc
     1321 gcgttccacc ccgctcgagt ccttcgtcgc caggcaggtc gtcaaggcgc tcaagagggc
     1381 tcgatacagc cctgacgaca tcgggcactt cggccttgcc ttcgatggat acctccactt
     1441 cacctcgccc atcaggaggt accccgacct ggtggtccac aggatcctca agggggtgct
     1501 ccggggcgag gggggcaggc cggaaaggac cgaccggaaa acgctggaga ggctggccca
     1561 ggagatgtcc atgcgcgagg agctcacgga caatgcgatg agggaggcgt tgaggctcaa
     1621 ggctgcatcc ttcatggcgc agcggatggg tgaggatttc gatgcggtgg ttaccgccct
     1681 caggcccggg gggcttttcg tggaggtcct cgacatcccc gtcgagggcg tggtgcgggg
     1741 ggactcgttc gacggcgggg gtcgtgccgt gcgcagaagg agagctcggc ctggcctcgg
     1801 cgtgggggat gtcgcgcggg tgcgcctcgt gcgggccgac accacgagcg gacagctcga
     1861 tttcgtcctc gttccgcccg aaggtcgaga cgcagccgag gccgggatcg acgcgcggcc
     1921 gaggcgaagc gcgcgaggaa ggcccggccg gggacggaaa gaccgctgaa gcccgttgag
     1981 gggcttgaca aacaggaggc gctttcctat aaaaatcgct ggcgcgggaa taactcagtg
     2041 gtagagtgta accttgccaa ggttaaagtc gcgggttcga atcccgtttc ccgctccagc
     2101 actcatccat gtgcatcgtc aagaggcgtc ccccactggt cgtgggggac gccttcgttt
     2161 cggtcgggcg accggccgag agggtcggcg agggcttcga ggcctcacgg gtaggcccta
     2221 tacagcatcc tcgggaacgg tatggtctcg cggacgtgct tgaggccgca gatccaggcg
     2281 agcgtccgct cgatgccgag cccgaagcct gagtgaggga cgcttccgta cttcctcagg
     2341 tcgaagtacc acccgtaacg ctccgggtcg agcccgttgt cgaggatcgc cttcatgagg
     2401 atctccgggt cgtcctccct ttcggacccc ccgatgatct cgccgtagcc ctcaggcgcc
     2461 agaaggtcgg cgcacaggac gagctccggg cgctcggggt gctgcttcat gtagaacgcc
     2521 ttggccttcc tcgggtagca ctcgatgaag acaggcttgt cgtagagctg tgtcaggatg
     2581 gtctcgtcgt cgccgcccag atcgtccccc cacacgatgt ccgaaccgtt cttccggagg
     2641 acctcgacgg cctcgtcgta gctgagcctg tggaacggag gctctatctt cttgaggggc
     2701 tcggtgtccc tctggagttt cgctagctcg ggggagcact ctcgcacggt gttctgcacg
     2761 atgtacgaca cgaagttctc ctggagctcg agattgtcct ggtgcctgga gaacgccgcc
     2821 tccgcctcca ccatccagaa ctcggtcagg tggcgcctcg tcttggatct ctcggcccgg
     2881 aaggtggggc cgaggcagaa gaccttgccc agtgcgaaga tcgctgcctc gaggtacagc
     2941 tggcccgtct gggaaaggta cgtcctgccc aggtcgaagt attcgaccga gaagaggttc
     3001 gacgagtcct cgccgacggt ggtcgtgaag atgggggtgt cgatgagggt gaagccgttt
     3061 tcgtggaaga agtttctgac gtgacggacc agcgcgtcgc ggatcctgag gatggcgacc
     3121 tgcctcgggg agcggatcca gaggtggcgc agccccagga ggaagtccgg gccgtgttcc
     3181 ttcttgccga tcgggaactc ctccgacttc tggaagatct tgatgccgct cgcgtggagc
     3241 tcgacccccc cctcggacct cggttcggcg accacggtgc ccgtgagttc caccgaggat
     3301 tcgagcggga gcttcctcac ctcttcgtag tcctcgccca gcgcctcccg ctcgacgacg
     3361 gcctggcaga aaccggatcc gtcgcggatg at
//
LOCUS       DAOSCR010000270         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17852_length_3391_cov_3.579736, whole genome shotgun sequence.
ACCESSION   DAOSCR010000270 DAOSCR010000000
VERSION     DAOSCR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17852_length_3391_cov_3.579736"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLT69_07385"
     CDS             <1..273
                     /locus_tag="PLT69_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14296.1"
                     /translation="EAEWVHPGFFIHELNFMKAGSLDGPIAALARVRYRQEAQPAVVH
                     PLRGGGAWVEFGGIVAPGQLAVFSDEDGDVLFAGVIERPGAFDPGA"
     gene            359..1123
                     /locus_tag="PLT69_07390"
     CDS             359..1123
                     /locus_tag="PLT69_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ14297.1"
                     /translation="MERKDGQPVITIGGRAHTLEEAWEVAAMGYPFVEVSLEDPGLVT
                     SHTPRLLELRSEYGIDLLAHFPNEDNPFDAGVLRAGFLPRIKSLIDLCPGLGIHKATV
                     HFWIDGRFAGVDLVSRKLEILAEIVEHAQGKGVVICIENLSERVDSFEPAFRAIPGLM
                     MTLDIGHAQLLSRTNTSFGFIERCRERIAHVHVHDNHGGTSVKDDLHLALGDGMVDYR
                     TIMAALVASGYDSTVTMEVALCDMPRTREALLGCIE"
     gene            1221..2912
                     /gene="recJ"
                     /locus_tag="PLT69_07395"
     CDS             1221..2912
                     /gene="recJ"
                     /locus_tag="PLT69_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOJ14298.1"
                     /translation="MVRVARSWVIRGGVDEEALKSLNFTAAFRSLMKIRGMVDPDQVM
                     GFLTPNLGQLRDPVDMKGVAEACDRIVRALERDETIGVFTDYDVDGVSSAVLIHRFLV
                     GVGAKPPMVFIPDRSSDGYGLNRRGIDELAARGVSLLITADCGVCSREEVAYARFLGM
                     DVIVTDHHEPDGDVPDACAVINPKQNDCLFFGEDLCGAGVVFHLIIALRKALRERGHT
                     ALPNLKEELDIVAMATVADAVSLSGVNRILVKEGLTVINSRSRAGISALAEVAGLRKG
                     LLTRDLGFVIGPRINAAGRIADARKAFDLLTTDDAGVARRLALELDSLNRSRQAHEQK
                     VLEDALNMLRDNPPHPRVALVWGEGWHVGVLGIVASRLAAILSRPAIVISVSGAQGTG
                     SGRSVAGVDLHRALGSVSSLLNTFGGHRMAVGFTLDAPKIPDLASALGEALEGVEWGQ
                     DGPYEVDLKISPFDLTPAFMDELELLAPYGEGNPEPVFLIPAMEVVDARVAKDGLAKL
                     VLRHSNRLFHTLKTPFKEDLYERGRFLDVAFTPVKVRLNGHHYLYLSLKALARAR"
     gene            complement(2857..>3391)
                     /locus_tag="PLT69_07400"
     CDS             complement(2857..>3391)
                     /locus_tag="PLT69_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14299.1"
                     /translation="ESAILDTLRIIDEWPTIKQKIPPVDYCPVTVIPITEEIVKHHEL
                     SEVDLHIYDLIDDKRTVERIVRESLEPPFEALSSLVRLMDLGLVEVFPQGASDVRDST
                     IARGKTIAGLKKAALYGAFVLCLAALAFSGQSRLLHQDLVPKVIVSHLEYQHKLAATF
                     SAPGPGPSGTDTGSGGR"
BASE COUNT          558 a         1062 c         1193 g          578 t
ORIGIN      
        1 gaggccgagt gggtgcaccc cgggttcttt atccacgagc tgaacttcat gaaggcgggc
       61 tcgctcgacg ggccgatcgc cgcgctggcg agggtccgct accgccagga ggcccagccg
      121 gcagtcgtgc acccgctcag ggggggcggc gcatgggtgg agttcggggg catcgtcgcg
      181 ccggggcagc tcgccgtgtt ctccgacgag gacggcgacg tgttgttcgc gggtgtcatc
      241 gagcggccgg gcgcgtttga ccccggggcg tgaccggtaa ggtgctcact gcgcctgact
      301 tggcgggcat ccgcccggta ggcggcctgg agaggagaga gaatcctgcg ggatcggcat
      361 ggagcgaaag gatgggcaac ccgtgatcac catcggcggc agggcgcaca ccctcgagga
      421 ggcatgggag gtcgccgcaa tgggataccc gttcgtggag gtatccctcg aggacccggg
      481 cctcgtcacg tcccacacac cgcggctcct cgagctcagg agcgagtacg gcatcgatct
      541 cctcgcgcac ttccccaacg aggacaaccc gttcgacgcc ggtgtcctga gggcgggctt
      601 cctgccgagg atcaagtcgc tcatagacct gtgcccgggc ctgggcatcc acaaggcgac
      661 cgtgcacttc tggatcgacg gccgcttcgc gggcgtcgat ctggtgtcca ggaagctcga
      721 gatccttgcg gagatcgtcg agcacgcgca gggcaagggg gtggtcatct gcatagagaa
      781 cctgagcgag cgggtggaca gcttcgaacc cgccttcagg gccatcccgg ggctcatgat
      841 gacgctcgac atcgggcacg cccagctctt gagcaggacg aacacctcgt tcggtttcat
      901 cgagcggtgc agggagagga tagcccacgt gcacgtgcat gacaaccacg gcgggaccag
      961 cgtgaaggac gacctccacc tggccctggg agacggcatg gtggattacc gcaccatcat
     1021 ggcggccctc gtcgcctcgg ggtacgactc caccgtgacc atggaggtgg cgctttgcga
     1081 catgcccagg accagggagg cgctcctcgg gtgcatcgag tgacgaggcc cgcgggtgct
     1141 cccgcgatgc cgtgaatcca tagggtggcc ccgggtcgag cccccttgac ttggggggtg
     1201 cctggggata cacagggggc atggtacgcg ttgcccgatc gtgggtgatc cgcgggggcg
     1261 ttgacgagga ggccctgaag tccctgaact tcacggcggc cttccgttcc ctcatgaaga
     1321 tacggggcat ggtcgatcca gaccaggtca tgggttttct cacgccgaac ctgggccagc
     1381 tcagggaccc ggtggacatg aaaggggtgg ccgaggcgtg cgaccgcatc gtccgcgcgc
     1441 tcgagcgcga cgagaccatc ggcgtgttca cggactacga cgttgacggg gtgagctccg
     1501 ccgttctcat ccacaggttt ctcgtgggtg tcggggcgaa gccgccgatg gtcttcatcc
     1561 ccgacaggtc gagcgacggc tacggactca acaggagggg gatcgacgaa ctcgccgcgc
     1621 ggggggtgtc gctcctgatc accgccgact gcggcgtctg ctcccgcgag gaagtcgcct
     1681 acgcccgttt cctcgggatg gacgtgatcg tgaccgacca ccacgagccg gacggggatg
     1741 ttcccgatgc gtgcgcggtg atcaacccga agcagaacga ctgcctgttc ttcggcgagg
     1801 atctctgcgg tgcgggcgtg gtcttccacc tcatcatagc cttgaggaag gcgctgaggg
     1861 agcggggtca cacggccctg cccaacctca aggaggaact cgacatcgtg gccatggcca
     1921 cggtcgcgga cgccgtgtcg ctttccggtg tcaaccgcat actcgtgaaa gagggcctca
     1981 cggtcatcaa ctctcgttcg cgggcgggca tctcggcgct cgccgaggtg gccgggctga
     2041 gaaaggggct cttgacgcgg gatttgggtt tcgtcatcgg gccgaggatc aatgccgcgg
     2101 gcaggatagc cgacgcccgc aaggcgttcg acctcctcac caccgatgac gccggcgtgg
     2161 cccggcggct cgccctggag ctcgattccc tcaacaggtc caggcaggcc cacgagcaga
     2221 aggtcctcga ggacgccctg aacatgctga gggacaaccc gccgcacccc cgggtggccc
     2281 tcgtgtgggg cgagggctgg cacgtgggtg tgctcgggat cgtcgcatcg cgcctggccg
     2341 caatcctgtc gaggcccgcg atcgtcatct cggtgtctgg cgcacagggc acggggtccg
     2401 ggaggtcggt cgcgggggtg gacctgcacc gcgccctggg ctctgtatca tcgctgctga
     2461 acaccttcgg cggccacagg atggcggtgg ggttcacgct ggacgcgccg aagataccgg
     2521 atctcgcctc cgcgctgggg gaggcgctcg aaggggtgga gtgggggcag gacggcccct
     2581 acgaggtgga cctcaagatc tctcccttcg acctcacgcc cgcgttcatg gacgagctgg
     2641 aactcctcgc cccatacggc gaggggaatc ccgaaccggt gttcctcatc cccgccatgg
     2701 aggtggtgga tgcgagggtc gcgaaagacg gcctggcgaa gctcgttctc aggcactcca
     2761 accggctctt ccacacgctg aagacgccgt tcaaggagga tctctacgag cggggcaggt
     2821 ttctcgacgt tgcgttcacg ccggtgaagg tgcgcctcaa cggccaccac tacctgtatc
     2881 tgtccctgaa ggccctggcc cgggcgcgct gaacgtcgcg gcgagcttgt gctggtattc
     2941 gaggtgcgag acgatcacct tcggcaccag gtcctggtgc agcagcctcg actgtcccga
     3001 aaaggcgagt gcggccaagc acaggacgaa cgcgccgtag agggccgcct tcttcaggcc
     3061 cgcgatggtc ttcccgcggg cgatggtcga gtccctgaca tccgatgcgc cctgggggaa
     3121 cacctccacg aggcccaggt ccatgagcct cacgagcgag ctgagggcct cgaacggcgg
     3181 ttcgagggat tcgcgcacga tcctctcgac cgtcctcttg tcgtcgatca ggtcgtagat
     3241 gtggaggtcg acctccgaga gctcgtggtg cttgacgatc tcctccgtga tggggatcac
     3301 ggtcaccggg cagtagtcga ccggcgggat cttctgcttg atggtcggcc actcgtcgat
     3361 gatgcggagg gtatcgagga tggcgctctc g
//
LOCUS       DAOSCR010000271         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17855_length_3391_cov_3.109113, whole genome shotgun sequence.
ACCESSION   DAOSCR010000271 DAOSCR010000000
VERSION     DAOSCR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17855_length_3391_cov_3.109113"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3205
                     /gene="smc"
                     /locus_tag="PLT69_07405"
     CDS             <1..3205
                     /gene="smc"
                     /locus_tag="PLT69_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOJ14300.1"
                     /translation="TRRLTRSGESEYFINNVRCRLKDITDLFVDTGLGKNSYAIVEQG
                     KVKDIVQARPDEIRSLLEDAAEVGKFRVKRAEALRRLEGASRNLERVRDLLEEVSRQR
                     QELKAQAAKARKYQELRAEANELERLVMTSEIMDVSRRRDTCVAALETIDARLKEAHE
                     RASALKGRMVSLEEEVVEARNRLERISGERAEARGAQGAASRELEALKARLKDVVAAR
                     EMLVNKVEESLVAMREHVARKDSLCTEIAELRSTVENLGGRLEMTRETSERLREACES
                     TEAAYQEKRNALFALIAEYRSIEQREKESREGLAELDAVRAKRLMDSDALKEEGSALE
                     GEIQRLESDKKALEDAMEPLEREIGELGSVRSAVRDRLERAAERIRDLDLQLARVSTK
                     IDMLRRITGGDLDGSTNTTRDLNGSRKVGQSVWAKEGFENAVGKGLGTALDYVIVRDH
                     DEILSLGDIAAKAPGFVIESPYVTEHAPGPPENGGRVLGRLEDFIETDREHEQIVRAL
                     AGDLLVVDGIDTAVSLWRKGYRECGYVTRDGMIMEPTGVVRTAPESSRYADVLRAKNE
                     MNALEGLRGTLLREREEALGEHGDAKREAERLEECAVDAAGRKESLLGEIRSCTERLE
                     ALRARRRKNAEQVTLAEEDLERIGALREKTVRQCGELASLKAEQEKQRVELEGELRAL
                     DEQRRAAKDALARALEASKEAGDRLHAVQLELAQKEASLRALEEGIEEGRKARSKDEA
                     MLEELSHASAALGRDIENVSRLIEETGARSAMLEERYAEALPVYEEAQNRLSSCRQEE
                     KDLRGEIEELNRSREDVQLDLRQNEVTFSMAKERIEARFGPYEAVLPEGFDREEARKR
                     AFEIEARIERMGQINFASLEEFEQVQKRWEGLHVQYEDLVQASSRLKEVIRNIERQSA
                     RAFQETFTQVKEYFREIFSSMFGGGRADLVETPALEGEEPGIEIIASPPFKKLRGMSL
                     LSEGEKTLCAVSFLFALFKVNPAPFCILDEVDAPLDDANITRLNRLIRSFCEETQFIV
                     VTHNRHTMETADILYGVTFETPGISKVVSMSLNGAQG"
BASE COUNT          785 a          897 c         1224 g          485 t
ORIGIN      
        1 gaccaggagg ctcacgagat cgggagagag cgaatacttc atcaacaacg tgaggtgccg
       61 gctcaaggac atcaccgacc tcttcgtcga taccggcctg gggaaaaaca gctatgccat
      121 cgtggaacag ggaaaggtca aggacattgt ccaggcgcgg ccggatgaga tccgctctct
      181 cctcgaggac gccgcggagg tggggaagtt cagggtaaag cgggccgagg cgctcagaag
      241 gctcgagggg gcgtccagga acctcgaacg cgtcagagac ctcctcgaag aggtctcaag
      301 gcagcgccag gagctcaagg cgcaggcggc caaggcgagg aaataccagg agcttagagc
      361 cgaggccaac gagctggagc ggctcgtgat gacgagcgag atcatggacg tctcgagacg
      421 cagggacaca tgcgtggcgg ccctcgaaac gatcgacgcc aggctcaagg aggcgcacga
      481 gagggcctcg gccttgaagg ggcgcatggt ttccctggag gaggaggtcg tcgaggcgag
      541 gaacaggctg gaaaggatct ccggcgaaag ggccgaggca cggggtgcac agggtgcggc
      601 ctcgagggag ctcgaggcat tgaaggcacg cctgaaagac gtcgtggcgg cgagggagat
      661 gctcgtgaac aaggtggaag agtcactcgt cgccatgagg gaacacgtcg cccggaaaga
      721 ctcgctgtgc acggagatag cggagctgag aagcacggtg gagaacctcg ggggtcgcct
      781 cgagatgaca cgcgagacgt cggaacgcct cagggaagcc tgtgagtcca cggaagccgc
      841 ataccaggaa aagaggaacg ctcttttcgc actcatcgcc gagtacaggt cgatcgagca
      901 gagggagaag gaatcacggg aaggcctggc cgaactcgat gccgtcaggg caaagaggct
      961 catggactcg gacgcgctga aggaggaggg gtctgctctg gagggagaga tccaaaggct
     1021 cgagtcggac aagaaggctc tcgaagacgc catggagccg ctcgagcgcg agatcgggga
     1081 gctcggctcc gtgcgttcgg cggtgcggga ccggctggag agggcggctg aacgcatcag
     1141 ggatctcgat cttcagctcg cgagggtctc caccaagata gacatgctga ggaggatcac
     1201 cggcggggac ttagacgggt cgacgaacac gaccagggat ctgaacggtt cgagaaaggt
     1261 gggccagagc gtctgggcga aggaaggctt cgagaacgcg gttggaaagg gcctcggcac
     1321 ggcgctcgat tacgtgatcg tgagagacca cgacgagatc ctctccctcg gggatatcgc
     1381 agccaaggcc cccgggttcg tgatcgagtc cccctatgtg accgaacacg cgcccggccc
     1441 cccagaaaac gggggaagag tcttgggaag gctcgaggac ttcattgaga cggaccgcga
     1501 gcacgagcag atcgtgcggg cgcttgcagg agacctcctc gtggtggacg gtatagacac
     1561 ggccgtatcc ctgtggagga aagggtacag ggagtgcggg tacgtgacga gggacggaat
     1621 gatcatggag cccacgggtg tcgtcaggac agcccccgag tcctcgcgct acgccgatgt
     1681 cctgagggcc aagaacgaga tgaacgcgct cgagggcctg cgtgggaccc tgctcaggga
     1741 aagggaggaa gccctcggcg agcacgggga tgcgaagcgg gaggccgagc gtctcgaaga
     1801 gtgcgctgtg gatgcggccg gcaggaagga atcgctcctc ggggagatcc ggtcgtgcac
     1861 ggagaggctg gaagccctcc gggcgcgaag gcgaaagaac gcggaacagg taactctcgc
     1921 agaggaagac cttgagcgga taggcgccct gagggagaag accgttcggc agtgcgggga
     1981 gctcgcatcc ctgaaggccg agcaggaaaa gcaaagggtt gagctcgagg gtgaactgag
     2041 ggcgctcgac gaacagagac gggccgcaaa ggatgcgctg gccagggctc tcgaggcgtc
     2101 gaaggaggcc ggggaccgct tgcatgcggt ccagctggag ctcgcgcaga aggaagcgtc
     2161 cctgagggcc ctcgaagagg ggatagagga gggaaggaag gcgcggtcga aggacgaggc
     2221 catgctcgag gagctttcgc acgcgagcgc ggccctcggc cgggacatcg agaacgtatc
     2281 ccggctcatc gaagagaccg gggcccgctc ggcaatgctc gaagaacggt atgccgaggc
     2341 cctgcccgtg tacgaggaag cgcagaacag gctctcctcg tgcaggcagg aggagaagga
     2401 tctgcgcggg gagatcgagg agttgaaccg ctcgagggag gatgtccagc tcgacctcag
     2461 gcagaacgag gtcacgttct ccatggccaa ggaacgaatc gaggccaggt tcggtccgta
     2521 cgaggccgtg ctgcccgaag ggttcgacag ggaagaggcc aggaagaggg ctttcgagat
     2581 cgaggcgcgt atcgagcgca tgggacagat aaacttcgcc tcgctcgagg agttcgagca
     2641 ggttcagaag aggtgggaag gcctccacgt gcagtacgag gacctcgtgc aggcgagctc
     2701 caggctcaag gaggtcatca ggaacatcga gaggcagagc gcgcgcgcct tccaggaaac
     2761 cttcacccag gtcaaggaat acttcaggga gatcttttcg tccatgttcg gtggcggcag
     2821 ggcggacctc gtcgagacac cggccctcga aggggaagag cccggcatcg agatcatcgc
     2881 ctcgccgccg ttcaagaagc tcaggggcat gagcctcctg tccgaggggg aaaagaccct
     2941 gtgcgcggtg agcttcctgt tcgccctctt caaggtcaat cccgcaccct tctgcatcct
     3001 cgacgaggtg gacgcacccc tcgacgacgc gaacatcacc aggctcaaca ggctcatcag
     3061 gtcgttctgc gaagaaaccc agttcatcgt ggtgacccac aaccgccaca ccatggagac
     3121 cgccgacatc ctctacgggg tcaccttcga gaccccgggg atcagcaagg tggtgtccat
     3181 gtcattaaac ggcgcacagg gataaaagga ttcttcccaa agagaaaaaa tttgccattt
     3241 ttgcccgtgt tgggaagaaa agcggccgga gatgccgcga tagctatttg catgtaattt
     3301 gcagggagag cgcagaggtg gtgcggccat gacgaggttc atagcggtca cgtccggcaa
     3361 gggcggggtt ggaaagagca atttcgtgct g
//
LOCUS       DAOSCR010000272         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17893_length_3385_cov_2.465766, whole genome shotgun sequence.
ACCESSION   DAOSCR010000272 DAOSCR010000000
VERSION     DAOSCR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17893_length_3385_cov_2.465766"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLT69_07410"
     CDS             complement(<1..783)
                     /locus_tag="PLT69_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14301.1"
                     /translation="MRKLLILALASLSAVLWAYGCGGGGSGGGSSGSGDGYAIEALAY
                     LTRDTSRTYTFTERVEASWDDQRASEEHEKTFSYETVDAIPGVYGDFSAYQGPFTKET
                     VRIDGVETMVTYTDGSGNVIVSDDFSVFTSITGSTSSGTLPTSMVPGTAYTTTLRELL
                     YNSDASAGAFGVNTGSSVTTTTFTPVAVGTITLGGTPYRALEAKISLVVSINGTVTTT
                     TEYTGRQWFGEGLGLIKAVLSYEVQQSGITVEYTVTDELVSVE"
     gene            complement(1531..1833)
                     /gene="yjgA"
                     /locus_tag="PLT69_07415"
     CDS             complement(1531..1833)
                     /gene="yjgA"
                     /locus_tag="PLT69_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HOJ14302.1"
                     /translation="MRRDPGKDPEAAEAPGEVKSKTRRKKEMLALQKLGVRLVGLPEA
                     YLKTCGIPGELLDAVLAAKRIRSFQARRRQMQYIGVVMRGVDADLVARVIERFEKR"
     gene            complement(1796..>3385)
                     /locus_tag="PLT69_07420"
     CDS             complement(1796..>3385)
                     /locus_tag="PLT69_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOJ14303.1"
                     /translation="RVGVIAQPDTSSGTDILRLGEPRLFWGVTAGSVDSMVANYTPLK
                     KRRRSDDYTPGGLNTKRPDRATIVYSNLIRRFSSSGAPIVLGGIEASLRRVAHYDYWS
                     DSIRRSVLFDAKADALVYGMGEYTALEIARRLASGLDISQTAGTCTISKTRPEGATEL
                     PPFELVQDDKEAFTDMFLAFSELGRPGSKTTLCQRHGDRFLVHNPPPSAPTTSGLDRV
                     YGLGYEREVHPRDRLLGEVRAIETIRFSITTHRGCAGRCSFCSISVHEGPVVVSRSQA
                     SILEEASGMTRHPLFKGIIQDVGGPTANMYGMGCKKGAIGGCTARRCLYPEPCPALRF
                     GHGAQIELLRRLRRIEGVRKVFVASGIRHDLVVADEGNGMRYLEELAAHHVSGQLKLA
                     PEHCRKEVLRLMAKPGTLHLLEFRDRFRALSEKAHKRQFLTYYFLAAHPGCTREDMEA
                     LRAYTESKLFLVPEQVQIFTPTPSTLSTLMYYTRRDPFSGAEIFVERDARRKRVQKDI
                     LTSKGGQPDRAAGSRKRPRGG"
BASE COUNT          506 a         1129 c         1107 g          643 t
ORIGIN      
        1 ctccacggat acgagctcgt ccgtcaccgt gtactccacc gtgattcccg attgctgtac
       61 ctcgtaggag agcacggcct ttatgaggcc gagcccctcg ccgaaccatt gccgtcccgt
      121 gtactcggtc gtggtcgtca cggtaccgtt tatgctcacc accaggctga tcttcgcctc
      181 gagcgcccgg tacggggtgc cccccagcgt gatggtgccg acggcgacag gggtgaacgt
      241 tgtcgtcgtg acgctcgacc ccgtgttcac gccgaaggcg cctgccgagg cgtccgagtt
      301 gtagaggagc tcgcggagcg tggtcgtgta ggccgtgcca ggcaccatgc tcgtggggag
      361 cgtgcctgag gacgtgctgc ccgttatgct cgtgaagacg ctgaagtcgt cgctcacgat
      421 cacgttgccc gagccgtcgg tgtaggtgac catggtctcc acgccgtcga tgcgcacggt
      481 ctccttcgtg aagggcccct ggtacgcgct gaagtccccg tacacaccgg gtatcgcgtc
      541 cacggtctcg tacgagaagg tcttctcgtg ttcctcgctc gcccgttggt cgtcccacga
      601 cgcctcgacc cgctcggtga acgtgtacgt cctcgatgtg tccctggtga ggtaggccag
      661 ggcctcgatg gcatacccgt ccccgcttcc cgacgacccg ccgccggatc cccctccgcc
      721 gcaaccgtac gcccacagca cggccgacag gcttgcaagg gcaaggatga gcagtttcct
      781 catggggggt acctcgttgt cacgctctct cgaggagact catcgacacg ggcggccgag
      841 aacatgacac cctcgtcgcg aactcgtgca acccctcttg acgacacggg atcccatcgt
      901 ccgcaagcgg catgggcacc ttgggaggtg gcgcaggggc gctcgggaaa tgccgcccgc
      961 gatgacgcgt gcgccctccc gcatgcgttc ggtttccccc tcacgtgcat gcgccgttgc
     1021 gtgaggcggt tcgaaaagga agctcgcagt gcggcgcgtg tgcgcccgtg cgtgagccca
     1081 tcgtggctcc atggggaggt caggccgcca tgtccaggag gaagtcccgc cccgttcatg
     1141 gaggccggtg tggggtcgcg tctcgccgta cgcgatgcgc caccacgggc cgcaaccagg
     1201 ggcttcacct tggtgtcgtt cggcgcgggc ccgtcaggct cgctcccctc ggccgcccaa
     1261 ggccgcgacc catcctcgcc gtcacttaag ggcggcggtc ccggtcggtc tcgtcgtgtt
     1321 ccggttctgc gtgccgcccc gtccttcaag ggggcgaagg aggcctgcgt cccatgcatg
     1381 cgtgacgtac cgcttgagct ttaccagctc tcgtgcttca ggcatacgtc ccatgcatgc
     1441 gtggcgttga cgctgaaccc ttgatgatga acgtctcttc gtcggcttgg agaccgaggc
     1501 cggatcttcg ggggaccagc aaaaaaaacg ctaccttttc tcgaatcgct cgatgaccct
     1561 ggcgacgagg tccgcgtcca ccccgcgcat gacgacgccg atgtactgca tctggcgtct
     1621 tctggcctga aacgatcgga tcctcttggc cgcgaggacg gcgtcgagga gttccccggg
     1681 gatgccgcac gtcttcaggt acgcttcggg cagcccgacc aggcgcaccc cgagcttctg
     1741 gagggcgagc atctccttct tccgccgcgt cttgctcttg acttccccgg gggcctcagc
     1801 cgcctcgggg tcttttcctg gatcccgccg cacggtctgg ctgtcctcct ttggaggtga
     1861 ggatgtcttt ctggacccgc ttgcgcctcg catccctctc gacgaagatc tccgcgcccg
     1921 agaacgggtc acgccgcgtg taatacatga gcgtcgagag ggtgcttggc gtgggcgtga
     1981 agatctggac ctgctcgggg acaagaaaga gtttcgactc ggtgtatgcc cggagggcct
     2041 ccatgtcttc cctggtgcag cccgggtgcg ccgcaaggaa gtagtaggtg aggaactgcc
     2101 tcttgtgcgc cttctcggac agggcgcgga agcggtccct gaactcgagc agatgcaggg
     2161 tgccgggctt ggccatgagc ctgagcacct ctttccggca gtgctcgggc gcgagcttga
     2221 gctggcccga gacgtggtgc gccgccagct cctccaggta gcgcatgccg tttccctcgt
     2281 ccgcgacgac gaggtcgtgc ctgatgcccg atgcgacgaa gaccttcctg accccctcga
     2341 tgcggcggag cctccggagc agctcgatct gggccccgtg cccgaaccgc agtgccgggc
     2401 agggctcggg gtacaggcat ctcctggcgg tgcagccgcc gatcgcgccc tttttgcagc
     2461 ccatgccgta catgttcgcc gtgggcccgc ccacgtcctg gatgatgccc ttgaagagcg
     2521 ggtggcgggt catgcccgag gcctcctcga ggatggaggc ctggctgcgt gacaccacga
     2581 caggcccctc gtgcaccgag atcgagcaga acgagcacct gcccgcacac cccctgtgcg
     2641 tggttatgga aaagcggatg gtctcgatcg ccctgacctc ccccaacaag cggtctctcg
     2701 ggtgcacctc gcgctcgtag ccgagcccgt agacgcggtc gagaccgctc gtggtcggcg
     2761 ccgagggggg tgggttgtgg acgaggaagc ggtcgccgtg cctctggcag agcgtggtct
     2821 tcgacccggg acgaccgagc tcggagaagg cgaggaacat gtccgtgaac gcctccttgt
     2881 cgtcttgcac cagctcgaag ggaggaagct cggttgcacc ttccggtctc gtcttcgaga
     2941 tcgtgcacgt accggccgtc tgcgagatgt cgagacccga ggcaagcctg cgcgcgatct
     3001 cgagcgcggt gtactcgccc atgccgtaga ccagggcgtc cgccttggcg tcgaagagca
     3061 cgctgcgcct gatggagtcg gaccagtagt cgtagtgcgc cacccggcgc aggctcgcct
     3121 cgatgccgcc cagcacgatc ggtgcgcctg aagaggagaa ccgcctgatg aggttggagt
     3181 agacgatggt cgccctgtcc ggcctcttcg tgttgaggcc gcccggcgtg tagtcgtccg
     3241 acctcctcct cttcttgagg ggggtgtagt tggccaccat ggaatcgacg ctccccgcgg
     3301 tgacgcccca gaacagcctc ggttcgccga gcctgaggat gtccgtgccc gatgacgtgt
     3361 ccggctgggc tatcacgccc accct
//
LOCUS       DAOSCR010000273         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17912_length_3382_cov_3.170123, whole genome shotgun sequence.
ACCESSION   DAOSCR010000273 DAOSCR010000000
VERSION     DAOSCR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17912_length_3382_cov_3.170123"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLT69_07425"
     CDS             complement(<1..400)
                     /locus_tag="PLT69_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14304.1"
                     /translation="MRTTRTNRPGLERRTRRRQGAALPLLFLVAALLASVPATCRAQE
                     AGAADEYAEFTGMPVVRLDMETRDCPWCTGDVASLAQDIVPLREQDPFSPERLRQSVD
                     ALKASRRYELVEVDVSPEPGGVGVSFTLKPG"
     gene            complement(416..>3382)
                     /locus_tag="PLT69_07430"
     CDS             complement(416..>3382)
                     /locus_tag="PLT69_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOJ14305.1"
                     /translation="CRALTAPPLLSRDDARVRGRVSSSAGRLTIAKLEAQASGIEASL
                     SGLIDLAGASMDVTGQVSSPKSSTLLKRIAGVDGSGALRSGFRAAGPFKSPEIAFEAS
                     FSGGTIGDVRLGDVSLRGKVTRASTLEIEDLTIRNGGSTVAASGTVGLFAPFPRLAGD
                     PSLSLEVRLDHADPSSFVPGVPVSGAVDGTAEISGSVRSPRATFALEGSDMSVSGVDL
                     GKADARGNLEGGVLEVEGMGLSLGASRVEAYGSVTLLDARGRLLADPGLAMSAKGTGI
                     RLEDLWGKAQGSASFDATITGTLAHPQGSASLTSGKVTIMGQAFTSAEIALRADGDIV
                     WLDRAVLTIAPGENINARGFVTMGGAYEFSLGTPGIEARHIGALGRYESLGGIVFLTA
                     SGKGTLDNPTLSGRVSAASLKYGDTPLEDMTFSFDLSDKILSIQGDWDFHVDARYDLG
                     RGVLEAEALFAETELGQLFVLAGRKDFSGRLTGRLFARGNPERPRDMEATAEISSVDV
                     FHEGSLLVQARDVNAAYSKDRVNLPQTRVSLAQGGLIDIQASGDPTHSFVVDLDGVVP
                     LTSLGLFDDDFWDATGSVRVLAQVVTAGSKGTVKGTAGLEDLSWTIPANSQRIHSVNG
                     KIRFEGDRVSLEDITGRLDTGSFQLGGVVLLDGLRPKTAEVFCQARALPLAIPDTMDL
                     VMDLEASLDYGTSSPRLWADVSLTDGVYYRDARVDLFSGVIGRVLLRQREEELKAPRI
                     PPPLASIPLEVTVRRRGNVKVENNIASLDINPDLKIAGTLGQPVVNGRITVTEGVVTF
                     QNNDFEVTRGLIDFLDPNRTRPVVDISANTRVRDWEITIALEGRLEELKFTLSSRPEE
                     DPSDILSLLVVGKTSRELASQQSGLSVSPTGMIAELLTSTYGGRIKKTTTLDILEFKA
                     SEFATTTGEESMTLLVGKELSRRLTVKYEMTTKNAETVQKAIAEYKILENLLLGGYQG
                     TDGIFGVDIFYRRRFR"
BASE COUNT          522 a         1147 c         1131 g          582 t
ORIGIN      
        1 ggccgggctt gagcgtgaag gataccccca cgccgcccgg ctccggcgac acgtcgacct
       61 ccaccagctc atagcgcctg ctcgccttca gcgcgtccac gctctgcctg aggcgctcgg
      121 ggctgaacgg gtcttgctcc ctgagcggca cgatgtcttg ggcgagggag gcgacgtcgc
      181 ccgtgcacca cgggcagtct cgcgtctcca tgtcgagccg cacgaccggc atgcccgtga
      241 actcggcgta ttcgtcggcg gccccggctt cctgggcgcg gcacgtggcc gggaccgacg
      301 ccaggagcgc cgcgacgagg aacaggaggg ggagggcggc cccttggcga cggcgggttc
      361 ggcgctcaag gcccgggcgg ttggtcctcg ttgtccgcat ggagcccccc atgactcacc
      421 ggaaccttcg cctgtagaag atgtccacgc cgaatatgcc gtccgtgccc tggtagcccc
      481 ccaggagcag gttctcgagg atcttgtatt ccgcgatcgc cttctgcacg gtctccgcgt
      541 tcttggttgt catttcgtac ttcaccgtca gcctcctcga gagttccttg cccacgagga
      601 gggtcatgct ttcctcgccg gtggtggtcg cgaactccga ggccttgaac tcgaggatgt
      661 cgagcgtggt ggtcttcttg atccggccgc cgtaggtgga cgtgaggagt tccgcgatca
      721 tgcccgtggg ggagacggag aggcccgact gctgggaggc gagctccctc gaggtcttgc
      781 ccacgacgag gagggagagg atgtccgagg ggtcttcctc gggcctggaa ctcagggtga
      841 acttaagctc ttccagcctc ccctcgagcg ctatcgttat ctcccagtcg cggaccctgg
      901 tgttcgcgct gatgtccacc acgggcctcg tgcggtttgg gtcgaggaag tcgatgaggc
      961 ccctggtgac ctcgaaatcg ttgttctgga aggtcaccac cccctcggtg accgtgatcc
     1021 ggccgttcac gacaggctgc ccgagcgtgc cggcgatctt caggtcgggg tttatgtcca
     1081 acgatgcgat gttgttctcc accttcacgt tcccccttcg tctcacggtc acctcgaggg
     1141 ggatcgacgc caagggggga gggatcctgg gggccttcag ctcctcctcc ctctgcctca
     1201 ggaggaccct gccgatgacg cccgagaaca ggtccacccg tgcgtccctg tagtacaccc
     1261 cgtccgtgag ggagacgtcc gcccacagcc tgggcgaaga agtcccgtaa tcgagggatg
     1321 cctccaggtc catgacgagg tccatggtgt cggggatggc caggggcagg gcccgcgcct
     1381 ggcagaacac ctccgcggtc ttcggcctga gaccgtccag gaggacaacg ccccccagct
     1441 ggaacgaccc ggtgtccagg cggcccgtga tgtcctcgag cgagacccgg tcgccctcga
     1501 agcggatctt tccgtttacg ctgtggatac gctgggagtt ggccgggatg gtccacgaga
     1561 ggtcctcgag gccggcggtc cccttgacgg tccccttcga tcccgcggtg acgacctgcg
     1621 caaggacgcg gaccgagccc gtcgcatccc agaagtcgtc gtcgaacagg cccagcgacg
     1681 tgagcggtac caccccgtcc aggtcgacca cgaacgagtg cgtgggatcc cctgatgcct
     1741 ggatgtcgat caggccgccc tgggcgaggc tgaccctcgt ctggggcagg ttcacccggt
     1801 ccttgctgta ggcggcgttc acgtcgcgcg cctggacgag gagggagccc tcgtggaaga
     1861 cgtccacgga ggagatctcc gccgtcgcct ccatgtccct gggcctttcc gggttgccgc
     1921 gtgcgaacag gcggcccgtg agcctgcccg aaaagtcctt cctccccgcg aggacgaaca
     1981 gctggccgag ttcggtctcg gcgaacagcg cctcggcctc gaggacgccc cgcccgagat
     2041 cgtacctcgc gtccacgtgg aagtcccagt cgccctggat ggagaggatc ttgtcggaca
     2101 ggtcgaagga gaacgtcatg tcttccaatg gtgtgtcgcc gtacttcaaa gaggcggccg
     2161 agaccctgcc cgagagggtg gggttgtcga gcgtgccctt tcccgacgcg gtgaggaaca
     2221 cgatcccgcc gagggattcg taacggccga gcgcgcctat gtgtcgggcc tctatgccgg
     2281 gggtgccgag cgaaaactcg taggcgccgc ccatggtgac gaaccccctc gcgtttatgt
     2341 tctcgccggg ggctatggtc agcacggccc ggtccagcca cacgatgtcg ccgtcggcgc
     2401 gaagggcgat ctccgccgag gtgaacgcct ggcccatgat cgtgaccttg ccggaggtga
     2461 gcgatgcact cccctgggga tgtgcgaggg tgcccgtgat cgtcgcgtcg aaggaggcgc
     2521 tcccctgcgc ctttccccag aggtcttcga gcctgatgcc ggtgcccttg gcgctcatgg
     2581 cgagaccggg gtcagccagg agccttcccc gggcgtcgag gagggtcacc gagccgtacg
     2641 cctccaccct cgaggccccc agagagagcc ccatgccctc cacctcgagc acgccgccct
     2701 cgaggttgcc ccgcgcgtcc gccttcccca ggtccacacc cgagacggac atgtccgagc
     2761 cctcgagcgc gaacgttgca cggggcgacc gtacgctccc cgagatctca gcggtcccgt
     2821 cgacggcgcc gctcaccggg acacccggca cgaagctcga ggggtcggcg tggtcaagcc
     2881 tcacttccag cgacagggac gggtctcccg cgagcctggg gaagggggcg aacagcccga
     2941 cggtgccgct ggcggccacg gtcgaccccc cgttcctgat ggtgagatcc tctatctcga
     3001 gcgtggaagc ccgggtcacc tttcccctga gcgatacgtc gccgagcctc acatcgccta
     3061 tggttcctcc cgagaacgac gcctcgaacg cgatctctgg gctcttgaag gggcctgcgg
     3121 ccctgaatcc agacctcaat gcacccgagc cgtcgacgcc cgcgatccgc ttgagcaggg
     3181 tcgagctttt cggcgaggac acttgaccgg tgacgtccat ggatgcgccg gcgagatcga
     3241 tcagacccga gagggaggcc tcgatgcccg aggcctgcgc ctcgagcttc gcgatggtga
     3301 ggcggcctgc cgaggaggag acccggcccc gcaccctcgc atcgtccctg gagagcaagg
     3361 gcggcgcggt cagggcacgg ca
//
LOCUS       DAOSCR010000274         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_17947_length_3379_cov_2.689832, whole genome shotgun sequence.
ACCESSION   DAOSCR010000274 DAOSCR010000000
VERSION     DAOSCR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17947_length_3379_cov_2.689832"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLT69_07435"
     CDS             complement(<1..486)
                     /locus_tag="PLT69_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14306.1"
                     /translation="MTPAIKEPAMTQCNSMESRSSYIRYALQVFLAAAVVMSAWGAMA
                     AQSTSRVVVAVAPVDELAVSDAERGILLDLKAAAGLTLLEGDPDVTARLNYVHNAPGA
                     KKITAEVLAGGMPAGTQDITLSVAVGSGPEVVVVSSGVPAGPHEVFQGLSRGAYVSVP
                     VS"
     gene            complement(831..2219)
                     /locus_tag="PLT69_07440"
     CDS             complement(831..2219)
                     /locus_tag="PLT69_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ14307.1"
                     /translation="MGGHGFENERFTLLCVDDDPTVVAIIKRIFEKEGYDIHTASSGR
                     QALEIMGRVHIDAALVDYMMPEMDGLELISTMREAHPGTMVIMVTAHGGVQEAVQAIK
                     LGATDFMEKPFSVEAMRSRVGNLYRIWQLKRENERLKQKVEMRFGYESLVGDSTVMLR
                     LKELITKVGPTDASVLVEGETGTGKELVARAIHHQSRRCRGVFVPVDCGAIAQTVIES
                     ELFGHVKGAFTGAHTAAKGLIRAADGGTLFFDEVGDLPLPMQVKLLRTIQEREVRPVG
                     SAQSYPVDIRIVAATNKDLSEEVGKGRFREDLLYRIDVVHIRVPPLREHKEDIPLLAR
                     HFVMESSRDVSRVRDISPEAVELLERYDWPGNIRELANVIRRAAALGRSELIMPEDLP
                     PTIREAKGSPAGLVRPEGSRLRDYERAAIVNALSLSGGNRKKASRILGIGEATLYRKL
                     ARYGLASEPGGG"
     gene            3105..>3379
                     /locus_tag="PLT69_07445"
     CDS             3105..>3379
                     /locus_tag="PLT69_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ14308.1"
                     /translation="MAAGKIMPRFDMKEGRLVKGMHFLDIRDAGNPVEHALFSRNDGA
                     DELAVPGIAATLGNRTTKLEWGKQVTSVTDIPLTVGCGIGTPADTEAV"
BASE COUNT          588 a         1164 c         1008 g          619 t
ORIGIN      
        1 cgagacaggg accgaaacgt aagcccccct cgataagcct tgaaacacct cgtgtggccc
       61 ggcaggcacg cctgaactca ccacgacgac ctcgggaccc gagcccaccg ccacgctgag
      121 cgtgatgtcc tgggtaccgg ccggcattcc gcctgccagc acctcggccg tgatcttctt
      181 tgcgccgggc gcgttgtgca cgtagttgag ccgggccgtg acgtcagggt cgccctcgag
      241 cagggtgagg cccgcggccg ctttcagatc gaggaggatg cccctttcgg cgtctgagac
      301 cgcgagctcg tctaccggcg ccaccgcgac cacgaccctg ctggtggact gagcggccat
      361 ggccccccat gccgacatga ccacggccgc cgccaggaat acctgcaatg catatcgtat
      421 atagctgctc ctggattcca tggaattgca ctgcgtcatg gcaggttcct taattgcagg
      481 ggtcatgcca cctggcctca ccagctatca tgttgatatt ccttgcatta cccgaaacga
      541 caccatctcc cgcgtctatc attttgagag tcgcccagga caggctctca ttttgagtca
      601 tggcccttcc gcgttgacca ccgcggtcgg ccgcacaggc ctgtcacagg gtcccgcacc
      661 acgcgggcac cgaggccacg cgcctccccg aagaacatgg aaaggaccga gaggcggccg
      721 agaggaagac cgcgcatcca ccccgtgacc ggcagacggc cccacgcgca acgcatgggg
      781 ccgggcgcct tcacggatac agccgcacgc aagggcctcg gccggcgggc tcatccaccc
      841 ccaggctctg aggccagccc gtaacgggcg agcttccggt agagggtggc ctccccgata
      901 cccaggatgc gcgaggcctt cttcctgttg cccccgctca aggagagcgc gttgacgatc
      961 gccgccctct cgtagtccct gaggcgcgat ccctcgggcc gcacgagacc cgccgggctg
     1021 cccttggcct ccctgatggt gggcgggagg tcttcgggca tgatgagttc agacctcccg
     1081 agggccgcgg ccctcctgat gacgttcgcg agttccctga tgttcccggg ccagtcgtag
     1141 cgttcgagga gctccaccgc ctccggggag atgtccctca ccctggacac atcccgcgag
     1201 gactccatga cgaagtgcct tgccaacagc gggatatcct ccttgtgctc cctcagcggg
     1261 ggcacccgta tgtggaccac gtcgatcctg tagaggagat cctcgcggaa cctccccttg
     1321 ccgacctcct cgctcaagtc cttgttcgtc gcggccacga tcctgatgtc gacgggatag
     1381 ctctgggcgc tgcctacggg cctgacctcg cgctcctgga tggtcctcag gagcttgacc
     1441 tgcatgggaa gcggcaggtc cccgacctcg tcgaagaaga gggtgccgcc gtcggccgcc
     1501 cgtatgagac ccttcgcggc ggtgtgggcc cccgtgaacg cgcccttcac gtggccgaag
     1561 agctcgctct cgatcacggt ttgtgcgatg gccccgcagt caacgggcac gaacacgccc
     1621 cgacaacgcc tgctctggtg gtggatggcc ctcgcgacga gttccttgcc ggttcccgtc
     1681 tcaccctcga cgagcaccga tgcatccgtg gggcccacct tggtgatgag ctccttgagc
     1741 ctgagcatga ccgtcgagtc tccgacgagg ctctcgtagc cgaagcgcat ctcgaccttt
     1801 tgcttgagcc tctcgttctc cctcttgagc tgccatatcc ggtaaaggtt ccccaccctg
     1861 gacctcatgg cctccaccga gaacggcttc tccatgaagt ccgtcgcgcc gagcttgatg
     1921 gcctggaccg cctcctggac tcccccgtgc gccgtgacca tgatcaccat ggtcccgggg
     1981 tgggcctcac gcatggtgga gatcagctcg aggccgtcca tctcgggcat catgtagtcc
     2041 acgagcgcgg cgtctatatg caccctgccc atgatctcga gggcctgcct gccgctcgat
     2101 gccgtgtgga tgtcgtaccc ttccttctcg aagatgcgct tgatgatggc gaccacggtg
     2161 gggtcgtcat ccacgcacaa gagcgtgaac ctctcgttct cgaaaccatg acctcccacg
     2221 ggcgcctccc cttgcgaact ccctccgcga ccgagtctat caaaatgata gccacatgcg
     2281 caacccgctt tcagccgcgc ccgtgacgtg acccctcttc gccttcgcaa acgtcgtgga
     2341 ctttccacaa aagtaccatc ttcgatcaag gtgcgtggca ttcaccgtca aggtattcct
     2401 tgcgtcggtg cgtttctgcc gagcgagcgc tctctttctt ggagcgtttg gaggcaaggc
     2461 gggcatcatg gagtgacttc ccggggtccg cacccagacc cttaggatcc atttcatgta
     2521 ccgaggagtg ctcatggcgt atgaaaccct cttgcttgca cagcggtgtc agatcgcggt
     2581 gctccccctg aaccggatac ccaccgactc ggtgagcccc gccgtgctcg gcgatatcga
     2641 caaggccctg gccgcagggc ccctcgatac agtctttgac ccaggcgatc tcacgggaaa
     2701 ggacacccag acctcgtcct cacatgcaga gcgcccctgc ctcggcaagg ggcatcgtcc
     2761 gcacggcaaa cgcgtactgc caccaggggg ccgaaaaggg gtcgaggccg tggcgggagg
     2821 gtatcaatgg ccctggccag ggttttgttg cagcgcaaaa aagattccca ggtcccgacg
     2881 gtacggggaa gaccctcttc cctgtacttt tggcatggcg ccgagatcca ggagagaaga
     2941 ccgcgtcctc ctgccgcagg tacgcccggc caagcccacg ggcctcattc caggcacccg
     3001 gcgacagtgg cccgggccgc gctacggggt agcgaggctt gatctttttc ccgcatgccg
     3061 gtacactgga tgtcctcgat gcacatcccc acgaggatgt tcccatggca gcaggaaaga
     3121 tcatgccgcg tttcgacatg aaggagggca ggctcgtcaa gggcatgcat ttcctagaca
     3181 tccgcgacgc gggaaacccg gtggagcatg ccctgttctc ccggaacgat ggcgcggacg
     3241 agctcgccgt gccgggcatc gccgcgacgc tgggaaaccg tacgaccaag ctggaatggg
     3301 ggaaacaggt cacctcggtc accgacatcc cgctcaccgt gggctgcggc atcggcacgc
     3361 cggccgacac cgaggcggt
//
LOCUS       DAOSCR010000275         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18088_length_3362_cov_3.025401, whole genome shotgun sequence.
ACCESSION   DAOSCR010000275 DAOSCR010000000
VERSION     DAOSCR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18088_length_3362_cov_3.025401"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..781
                     /locus_tag="PLT69_07450"
     CDS             92..781
                     /locus_tag="PLT69_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14309.1"
                     /translation="MRKTVLQSAMLFVLLFCFQTATSAQGPMMGSPRLKGEFKPVLGA
                     WAQYRLKGGDMPSSTMKIAIVGKEGSYYWYETITEQGGETTIAKMLVSGDPGNSKAVK
                     RFIVKNGGEPAMEMPVTESAKPAGPREAPCTMVDKGNETVSVPAGTFRARHLQFTCGN
                     ERGDTWASEKVPPYGIVKSTTRGMEMILTGYGMGAKTAITETPRKFTMPPMPKGMPKG
                     MMPPGMVPPGD"
     gene            900..2234
                     /locus_tag="PLT69_07455"
     CDS             900..2234
                     /locus_tag="PLT69_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ14310.1"
                     /translation="MKTMFTLQETPFPLVMQEIVKDSFTGIVFVSSDAWKKGLIFKGG
                     GLCAIQSNRTEELLGNILVEMGFISAEENERSLARSRTERRKQGVILLEMGLVQSRDI
                     NEALAVQTEKRFLDIFSWEDGLVQVVPKQDIEKNPLVGGHQLARLIRRGVMEYAPFSA
                     VITSLSPFADATPKVLVDSLPADVGVDLLDMSHYKVSEILLLGQDPSRALLGLYCTGD
                     VSFEESRYKALIDTLRQKLKTIKDQDPFQVLGVDRQISDGGLKRAYIKIVKANHPDTY
                     AHADDPEVRRLANEVFTEIQKAYSAVTKIRAGQSVEEPKGIDESIQAEILYGKGLEYL
                     KARDYNKAIDCLKLCMKLRPEERVFTETYVKTLFLRLQNTGTGSSIEIKTAIRDGLNR
                     FPSSDTLWLIYGWLLKREGSKKAVEAFKKALEINKDNVEAQRELRLYQMREN"
     gene            complement(2206..2556)
                     /locus_tag="PLT69_07460"
     CDS             complement(2206..2556)
                     /locus_tag="PLT69_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14311.1"
                     /translation="MDDRKGRELFAVLYEAATASGVEVIQDRLNRLGGVCRVNGRVCV
                     ILDERAPYREKNRLVIEGIAMVDYDAAYLPPKVRELVTRAVFVRGRGLATGPGEEAPS
                     ERPKGPSSPSSGKA"
     gene            complement(2569..2904)
                     /locus_tag="PLT69_07465"
                     /pseudo
     CDS             complement(2569..2904)
                     /locus_tag="PLT69_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
     gene            complement(3092..>3362)
                     /locus_tag="PLT69_07470"
     CDS             complement(3092..>3362)
                     /locus_tag="PLT69_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOJ14312.1"
                     /translation="AKSFCCGAGGGRMWMEEHLGTRINQKRTRQALEAGAKTVCTACP
                     FCYTMLTDGIKELELGEQLQSADIAILVARAAGIDLKPKKTEGDA"
BASE COUNT          664 a         1110 c         1010 g          578 t
ORIGIN      
        1 gaaggcgcat aatatgatcc gactcaatct tcggctccag ggtgctcaga caggcgaaga
       61 ccctgtgagc cggacgtacg gggaggtggc catgaggaaa acggttcttc agtctgccat
      121 gcttttcgtc cttctgttct gcttccagac ggcgacgtcg gcccagggcc cgatgatggg
      181 atccccccgg ctgaagggcg agttcaagcc cgtgctcggc gcctgggcgc agtaccgact
      241 caagggcggg gacatgccct cttccaccat gaagatcgcg atcgtcggca aggagggatc
      301 gtactactgg tacgagacga tcaccgaaca gggtggagag accaccatag ccaagatgct
      361 cgtctcgggc gaccccggca acagcaaggc ggtcaagagg ttcatcgtca agaacggggg
      421 cgagcccgcc atggagatgc cggtcacgga gagcgccaaa ccggcaggtc cccgcgaggc
      481 accctgcacc atggtggaca aggggaacga gacggtgagc gtccctgcag ggacgttcag
      541 ggcgaggcac ctccagttca cctgcgggaa cgagcggggg gatacgtggg cgagcgagaa
      601 ggtccccccc tacggcatcg tgaaatcgac gacgaggggc atggagatga tcctcaccgg
      661 ctacggcatg ggcgcgaaga ccgccatcac ggagacgccg cggaaattca ccatgccgcc
      721 catgcccaag ggaatgccga agggcatgat gccgcccggc atggtacccc ccggcgattg
      781 atcggcccct cccggtgggc cgtggcccgt ggccgcacgc gccccgtctg cctagagcga
      841 agaaggtttg aaatgtgttt ctgtgcggca ggagacctgc cgataactat ggagagatca
      901 tgaagacgat gttcacgcta caggaaaccc cgtttccgct ggtcatgcag gagatcgtca
      961 aggactcctt cacggggatc gtcttcgtat cgagcgacgc ctggaaaaag gggctcatct
     1021 tcaagggagg ggggctctgc gcgatccagt ccaacaggac ggaggagctg ctcgggaaca
     1081 tcctcgtgga aatggggttc atctcggccg aggagaacga gcgctccctg gcaaggagtc
     1141 gcaccgagcg ccgcaagcag ggcgtgatcc tcctcgagat ggggctcgtg cagtccaggg
     1201 acatcaacga ggcgcttgcg gtccagaccg agaagcggtt cttggacatc ttttcgtggg
     1261 aggacggtct cgtccaggtg gtccccaagc aggacatcga gaagaacccc cttgtgggtg
     1321 gacaccagct ggcacgactc atcaggagag gggtcatgga gtacgccccg ttctcggcgg
     1381 tcatcacctc gctctcgccc ttcgccgacg caacacccaa ggtcctcgtg gacagcctgc
     1441 cggccgacgt cggcgtagac ctcctcgaca tgtcgcacta caaggtctca gagatcctgc
     1501 tgctcgggca ggacccgtcg agggctcttc tcgggctcta ttgcacgggc gatgtcagtt
     1561 tcgaggagag caggtacaag gccctcatag acaccctcag gcagaaactc aagaccatca
     1621 aggaccagga cccgttccag gtcctcggcg tggacaggca gatctctgac ggcgggctca
     1681 agcgcgccta catcaagatc gtcaaggcca accacccgga cacctacgca catgccgacg
     1741 accccgaggt gagacgcctc gcgaacgagg tcttcaccga gatccagaag gcgtactctg
     1801 cggtcacgaa gatccgcgca ggccagagcg tggaagaacc caagggcatc gacgagagca
     1861 tccaggcgga gatcctctac ggcaagggcc tggagtacct caaggccagg gactacaaca
     1921 aggcgataga ctgcctgaaa ctctgcatga aactcaggcc cgaagagagg gtcttcacgg
     1981 agacctacgt caagacgctc ttcctcaggc tccagaacac cggcacaggg agctcaatcg
     2041 agatcaagac cgccatccgt gacgggctga accgtttccc gtcttccgac accctgtggc
     2101 tcatctacgg ctggctcctc aagagggaag gttctaagaa ggcggtggag gcgttcaaaa
     2161 aggccctcga gatcaacaag gacaatgtgg aggcccagcg cgagctcagg ctttaccaga
     2221 tgagggagaa ctagggccct tgggcctctc tgaaggtgct tcctccccgg ggcctgtcgc
     2281 aaggccgcgt ccccgcacga acaccgccct cgtcacgagc tccctcacct tgggcggcag
     2341 gtacgcggcg tcgtaatcca ccatcgcgat accctctatc acgaggcggt tcttctccct
     2401 gtacggcgcg cgctcgtcga ggatcacgca cacccgcccg ttcaccctgc atacgccgcc
     2461 taggcggttc agcctgtcct ggatcacctc cacgcccgac gccgtggcag cctcgtagag
     2521 taccgcgaag agttccctcc ccttcctgtc gtccacgccc tcttcccctc aaacgcgtcc
     2581 gtaatcgtcc tcgagcctca cgatgtcgtc ctccccgaag tagtcgccca tctggacctc
     2641 tatgaacacg aggggttctt ccccgacgtt ctccacccgg tgcggcgccc cttgcgggat
     2701 gtccaccgac tggcccgggc ccaggcgcac gacctcttca cccacggtca cgagcgcctc
     2761 acccgccacc accacccagt gctcacgcct tcgcgagtgg tactggaggc tcagcctctt
     2821 tcccggcaat accgtgatgc gcttgacctt gtggtctgcc ccggtatcgg aaaggatctc
     2881 gaacgagccc cagggcctgt gttccatctc gtgcctcgtg tccaagagca cccccatcgc
     2941 cccctagccg ggtcacggca gggggcggct ctgccttgcg atgaccgtca tcttcgtccc
     3001 tgagataccc cccggcaggg gctgctgcaa gaaaaaaagg ggggcgcctt gcgcccccct
     3061 ccatgcaggt ccacttccga gaatgcccgc cttacgcatc cccttccgtc ttcttcggct
     3121 tcaggtcgat gcccgcagcc ctcgccacca ggatcgcgat gtcagccgac tggagctgtt
     3181 cgccgagctc gagctccttg atgccgtccg tcagcatggt gtagcagaac gggcatgcgg
     3241 tgcagacggt cttcgccccg gcttccaggg cctgcctggt gcgcttctgg ttgatcctgg
     3301 tccccaggtg ctcctccatc cacatcctcc cgcctcctgc tccgcagcag aagctcttcg
     3361 cg
//
LOCUS       DAOSCR010000276         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18135_length_3356_cov_3.624659, whole genome shotgun sequence.
ACCESSION   DAOSCR010000276 DAOSCR010000000
VERSION     DAOSCR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18135_length_3356_cov_3.624659"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..423)
                     /locus_tag="PLT69_07475"
     CDS             complement(82..423)
                     /locus_tag="PLT69_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ14313.1"
                     /translation="MKSRCMIVCASLFVFLLAAHLFAAQGVINVNTATVEQLMMLPGI
                     GEKTAKAIVSYRQAHGQFKTVDDLARVKGIGMKKVNALRPNLAVQGQNTYVPDAAGQK
                     RAQSGAKTTGS"
     gene            632..1654
                     /locus_tag="PLT69_07480"
     CDS             632..1654
                     /locus_tag="PLT69_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14314.1"
                     /translation="MERRKRRSLAAVIALSVLAACLVSGCGSESGSGSSSVAPGTPLE
                     LFSEDVEACTPLLEEASPDEGTYDISSWEGWDPEADKSVFGTLFDPGIGRIACIHKVA
                     EILDTHIGLVNTFSDEWSSSGTYTSAGMTAEVDTTVPQFVLPFLGFDSRLIALEKMVT
                     LRVPSDNLTLHMAFSQSGEGNQIVIEQYSKGQTEAGVFIAQRQASKTRIWYAGVSAGD
                     KVQLMWEGDTSGLSFMISVCTNAGGGNWEAMGGGFTSSGGDDIAVMARNNVAVQLEDE
                     YYVHLTLNALEEGAAGQVLLASDNPPGTSGALAYINVDMDGYPGLGYLGPDAYPEDAD
                     ALAWED"
     gene            complement(2073..2843)
                     /locus_tag="PLT69_07485"
     CDS             complement(2073..2843)
                     /locus_tag="PLT69_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ14315.1"
                     /translation="MKDRSPWLSVFVLLFVSLCVSTSGSDASAAQTLRLATLDWEPYV
                     GQTLKDKGFNTAIVTEAFRRAGYQVKIDFLPWDKAIEEAKKGAYDAVYPEYYSKERAQ
                     DFEYSYFFSNSLLVFYKKAQANITYKNLRDLATYRIGVVKGYINTDEFDNATYLKKVE
                     CESDEDNLRKLLKGEVDLIVIDKLVAQYLIDTKIPEAKGKLAPLEPPLTIHNLFVIFP
                     KSKNPASKKLATEFNKAFESMDKDGTVKAIMRATGLVK"
     gene            3112..>3356
                     /locus_tag="PLT69_07490"
     CDS             3112..>3356
                     /locus_tag="PLT69_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14316.1"
                     /translation="MMFASVDDVQVSLERVGYITSREIATIIFLANVTGKPLLVEGPA
                     GVGKTELAKAVSRAVPCELIRLQCYEGLDEAKALYEW"
BASE COUNT          618 a          998 c         1057 g          683 t
ORIGIN      
        1 ccctcggtcg tgacccacgg caggccttat caccaggtgc ccctttcccg ccggtctccc
       61 cggcaggggc cggggcgcga tctacgaccc cgtggtcttg gcgccggact gcgccctctt
      121 ctgaccggcc gcatccggaa cgtaggtgtt ctgaccctgg accgcgaggt tcggcctgag
      181 ggcgttgacc ttcttcatgc ctatgccctt gaccctggcg aggtcgtcca cggtcttgaa
      241 ctggccgtgg gcctggcgat acgaaacgat ggccttggcc gtcttctccc cgatcccggg
      301 caacatcatg agctgctcga cggtggcggt gttcacgttg atcacaccct gtgcggcaaa
      361 gaggtgggcg gccaacagga acacgaagag agacgcgcaa acgatcatac accttgattt
      421 catgggaaca gacctccttt caaagggatc tcaagaaacc gcatgggtat gatcccattg
      481 tgaaatgccg tggacgtcga gtcaacggtt ctgagtgaca ggaaagttgc aggaaatcgt
      541 taggtctgcc ctgttttcgt ttttgaactt gatttatggg ccatgtgaga atagccgtat
      601 aggtgggtat cgcacgagca tgggaggcct catggaacga agaaagagga gatcgctcgc
      661 ggccgtgatc gcgctgtcgg tgcttgcggc ctgcctcgtt tcgggttgcg ggagcgagag
      721 cgggtccgga tcgagctcgg ttgcgccggg tacaccgctc gagctcttct ccgaggacgt
      781 ggaggcgtgc accccgctcc tcgaggaggc gagccccgat gaggggacgt acgacatcag
      841 ctcgtgggag gggtgggacc ctgaggccga caagtcggtc ttcggcaccc tcttcgaccc
      901 cggaatcgga cgcatagcct gcatccacaa ggtggccgag atcctggaca cccatatagg
      961 gctggtaaac accttctccg atgagtggtc ctcttcgggc acctacacct ccgccgggat
     1021 gacggcggag gtcgatacga ccgtgccgca gttcgtcctg cccttcctgg ggttcgactc
     1081 gaggctgatc gctctcgaga agatggtcac gctcagggtg ccctcggaca acctgacgct
     1141 gcacatggcc ttttcccagt cgggagaagg aaaccagatc gtcatcgagc agtattccaa
     1201 ggggcagacg gaagcagggg tgttcatcgc ccagagacag gcatcgaaga cgcggatctg
     1261 gtatgcgggt gtctctgcag gggacaaggt ccagctcatg tgggaggggg acacctccgg
     1321 gctttccttc atgatctccg tgtgtacgaa tgcaggcgga ggaaactggg aggcgatggg
     1381 tggcgggttc acgtcatccg gcggcgacga catcgccgtc atggcccgaa acaacgtggc
     1441 agtccagctc gaggacgagt actacgtaca cctcaccctg aacgcgctcg aggagggggc
     1501 tgcagggcag gtcctgctcg cttccgacaa ccccccgggc acgagtgggg ccctggcgta
     1561 catcaatgtg gacatggacg ggtatccagg cctcgggtac ttaggcccgg acgcgtatcc
     1621 cgaggatgcg gacgcactcg cctgggagga ttgaggcgca taccctgcct gagccaggcc
     1681 acgaccgggg cgccctacac cccatgcgct cgtaggacat atgcagggat ccccacctcc
     1741 acagcctgag aagaggcctg gggccggcct cttttctatc ccacgcatcg ccgttttcta
     1801 ccatgggcat gtctcggccg gggtcccggc ggcgccgcga gattcttctc tcccaggcgc
     1861 cctcgcctaa gcgtttacgg gacggaaaga cagagtgtgg gcgggacccc ggtgacgggt
     1921 tctctccttc aagaacacga caggctttgt ggcaccggtg cgtgcgacgg gatgacgctt
     1981 tcagccatgc ccgggcgcca gacccccacg atgcggcctg cccccgtatc gtgccgaggt
     2041 gccgatgagg aggcaggccg cacggggcgg cgtcacttca cgagaccggt agcgcgcatg
     2101 atggccttga ccgtcccgtc cttatccatc gactcgaagg ccttgttgaa ctcggtcgcg
     2161 agtttcttgg aagccgggtt cttcgacttg gggaagatga cgaagaggtt gtggatggtc
     2221 agcgggggtt ccaagggggc aagcttgccc ttggcctcgg ggatcttcgt gtcgatgagg
     2281 tactgggcca cgagcttgtc tatcacgatg agatcgacct cccccttgag gagctttctc
     2341 aggttatcct cgtcgctttc gcattccact ttcttcaggt aggtggcgtt gtcgaactcg
     2401 tcggtgttga tgtacccctt caccacgcct atccggtatg tcgccaggtc tcggagattc
     2461 ttgtaggtga tgttcgcctg ggccttcttg tagaagacga ggaggctgtt cgagaagaag
     2521 taggagtact cgaagtcttg cgcgcgttcc ttcgagtagt attcgggata gacggcgtcg
     2581 tacgcgcctt tctttgcctc ttctatggcc ttgtcccaag gcaggaagtc gatcttcacc
     2641 tggtagccgg ccctcctgaa ggcctcggtg acgatcgccg tgttgaaccc cttgtccttg
     2701 agcgtctggc ccacgtaggg ttcccagtcg agggtggcga gcctgagggt ctgggcagcg
     2761 gacgcgtccg atccggacgt cgatacacac agcgagacga acagcaggac gaacacggac
     2821 aaccaaggtg acctatcctt catgcgaacc ctccttttgt tttttcgggc gcagaacgga
     2881 ctgtcccgtc ccgacgccat cgttctcatc acgtatcatc ccgctcggcg cgggaaagac
     2941 ctcgtggcga tgaacccatc acaccctctc accctatgcc cccctcgaga ggttttgcaa
     3001 tcccctgtgt cgccccggtc ctcattagac ctcgaccgat gagccgaaac ggtgtttctt
     3061 tcggatgggg cttggtgtat agaataatgc tccacgagaa aggatcaagc catgatgttc
     3121 gcaagcgtcg acgatgtcca ggtctccctc gaaagggtcg ggtacataac atccagggag
     3181 atagccacca tcatcttcct cgccaacgtg acgggcaaac ccctgcttgt cgaggggccc
     3241 gcgggcgtcg gcaagaccga gctggccaag gcggtctcga gggccgtccc atgcgagctc
     3301 atccgtctcc agtgctacga ggggttggac gaggccaagg cgctctacga gtggga
//
LOCUS       DAOSCR010000277         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18143_length_3355_cov_3.379697, whole genome shotgun sequence.
ACCESSION   DAOSCR010000277 DAOSCR010000000
VERSION     DAOSCR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18143_length_3355_cov_3.379697"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLT69_07495"
     CDS             <1..560
                     /locus_tag="PLT69_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOJ14317.1"
                     /translation="GDTGAYIRTTMQAMVLFGVPPEEYWPYRVSEFDREPSAFCYAFA
                     QNYQAIQYIRLDPSGITRDELLKVVKATIAAGIPAMFGFTVYESIHEADDGRIPFPGP
                     DENVLGGHAVMAAGYDDAIEIANPSTGKATKGALLIRNSWGKGWGESGYGWLPYDYVT
                     RGLAVDWWTLLKSEWIDTGEFRLGG"
     gene            complement(1263..2798)
                     /locus_tag="PLT69_07500"
     CDS             complement(1263..2798)
                     /locus_tag="PLT69_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14318.1"
                     /translation="MLVQHFLEHSARRLPDKTALVFGNERLTYGALNALANRFAHALM
                     EAGLLRGERVAVFLDNSIETVISIFGTLKAGGVFSVLSPTMKERKLSYILNHLDASFL
                     VTHASKMNQAAPASADAASLRAVITSGGGGVSPHTPVSILDWDGFIRGKPDHEPSCPS
                     IDIDLATIIYTSGTTADPKGIMMTHLNMTHVSSSIISYLQNDEADVIMDVLPLSFDYG
                     LYQALMSLRIGGTLVLERSFSFPYAIVERMAGERVTGFPGVPTVFAMLLRLESFNGRI
                     LPHLRYITNTGAPLPVRHIQLLRERFPNVALYSMYGLTECKRVSYLPPSEIDRRPESV
                     GIPIPNTEVFIVDEHGNRVEQGMVGELVVRGAHVMKGYWKDPEETAKRLRPGLLPDEK
                     VLFTGDLFRMDEDGFLYFIARKDDLIKTRGERVSPREVENAIHEIPGVVEAAVIAVPD
                     EILGSAIKAFVVVESPAGLTERDVIAHCAKTLETFMVPKYVEFVSDLPRTSSGKVDKK
                     ALR"
     gene            2884..>3355
                     /locus_tag="PLT69_07505"
     CDS             2884..>3355
                     /locus_tag="PLT69_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ14319.1"
                     /translation="MKGIILAGGSGTRLFPITRVVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILVISTPSDLPRFIDLLGDGSQWGVSFSYEEQPRPEGIAQAFIIGERFVGSDTV
                     SLVLGDNIFYGTGLKERLMGASSIERGSVVFGYWVSDPERYGVVNFDERGTPVDI"
BASE COUNT          676 a          972 c         1008 g          699 t
ORIGIN      
        1 cgggcgacac cggggcatac atccgcacca ccatgcaggc gatggtgctc ttcggcgtac
       61 cgcctgagga atactggcca taccgggtct cagagttcga cagggagcct tctgcgttct
      121 gctacgcctt cgcccagaat taccaggcca tccagtacat acggctcgac ccttccggca
      181 tcacgcggga tgagctgctc aaggtcgtca aggcgacgat cgccgccggg atccccgcga
      241 tgttcgggtt cacggtgtac gagtccatac acgaggctga tgacggcagg atccccttcc
      301 cggggccgga cgagaatgtc ttgggcgggc atgccgtgat ggcggcagga tatgacgacg
      361 caatcgagat cgcaaacccg tcgacaggca aggccaccaa gggtgcgctc ctgatacgca
      421 actcctgggg aaagggctgg ggcgagtccg gctacggctg gctgccctac gactacgtga
      481 ccaggggcct tgccgtcgat tggtggacgc tcctcaagag cgagtggatc gatactgggg
      541 aattcaggct cggcgggtaa ccaactaaag agacaggcag aaacaagggc cacttaaaaa
      601 tatgtgacag acagcttcga ggagaccccc ctccaacagc cccctctccg ccggtaaaag
      661 gcggggtagg gggcttttct tccataccat gtacgcactg tctctccagg aggacatgag
      721 cccggctttc tcgttcctga aaggatttta agttttgggg acgaaacatg gtcctgtatc
      781 ctgcgatctt ctcgatgggt gaggactgga acccgaagac cttcctgaac gtcactgcca
      841 caggatcagc gcccagtgcc tgattatgtc acggtgcagc gtctgaggct gagaacgtgt
      901 ctcgccggtg ggttgagtgc gccagcccac acgtagggtg tcttttcgga tactgagctg
      961 attcagggat cctgtacgaa tgggtaacct ctggaatccc tgggacaata cgacttgacg
     1021 cccagggtgg agtcccgcca ttggtcaagg tgacgacacc tgcacggggt ttcgggtatc
     1081 cctgcccatg gttcgatggc aacgaaggcg tgggtatatc ccccaaggaa cttctcccca
     1141 cgatctccca agcggtcagg ggcacctgcg ccctttgtag agtcctctcc tttgaagcca
     1201 tctcacggag ggtcttcacg ggatagactg gtgaacgagt tccttcggga cgtgagaacg
     1261 tatcaacgaa gggccttctt gtccaccttc ccggagctcg tgcgcggcag gtcgctcacg
     1321 aattcgacgt acttggggac catgaaggtc tccagcgtct ttgcacagtg tgcgatcaca
     1381 tccctctcag tgagccccgc cggagattcc acgaccacga aggcctttat ggcgctgccc
     1441 agaatctcgt cgggcaccgc gatgacagca gcctcgacga ctccggggat ctcgtgaatc
     1501 gcgttttcca cttctctcgg actcacccgt tcgccccttg tcttgatgag atcgtccttc
     1561 ctggcgatga agtacaggaa cccatcttcg tccatcctga acaggtcacc cgtgaaaagg
     1621 actttctcgt ctggaaggag acctggtctt aacctcttgg ccgtctcctc gggatctttc
     1681 cagtacccct tcatgacatg agcacctcgt accacgagtt cgcccaccat gccctgctcc
     1741 acgcggttcc catgctcgtc cacgatgaag acctcggtgt tcgggatggg gatgccgacg
     1801 gattccggcc ttcggtctat ttccgaagga gggaggtagg atacacgctt gcactccgta
     1861 agcccgtaca tggaatacag ggcgacgttc gggaacctct cgcggaggag ctgaatgtgc
     1921 ctgaccggca aaggagcgcc cgtgttcgtg atgtatctga ggtgtggcag gatccgcccg
     1981 ttgaaactct cgagcctcag gagcatggcg aatacggtgg gcactcccgg gaaacccgtg
     2041 accctctcgc ccgccatcct ttccacgatc gcatagggaa acgaaaagga cctttcgagc
     2101 accaaggtac cgccgatcct taggctcatg agcgcctggt aaagaccgta gtcgaacgag
     2161 agcgggagta cgtccatgat gacgtctgct tcgtcgttct ggaggtagga gattatggag
     2221 gatgagacgt gcgtcatgtt gagatgggtc atcatgatgc ccttagggtc cgccgtggtc
     2281 cccgaggtgt agatgatggt cgcgaggtcg atatctattg acgggcagct cggttcatga
     2341 tccggttttc ccctgatgaa accatcccag tcgagtatcg aaacaggtgt atgaggagag
     2401 actccgcccc caccgcttgt gatgacggca cgaagcgatg ccgcatcggc tgaagcggga
     2461 gccgcctggt tcatcttgga tgcgtgggtg accaggaacg aggcatcgag atggttcagg
     2521 atataggaga gtttcctttc cttcatggtc gggctgagca cggaaaagac gccccccgcc
     2581 ttcagggtgc cgaagatgct gatcacggtc tcgatggaat tgtccaggaa aaccgccact
     2641 cgttctcccc gcagcagtcc cgcctccatc agggcgtggg cgaacctgtt tgcgagagcg
     2701 ttcagggccc cataggtgag ccgttcattg ccgaacacga gggcggtttt gtccggcagc
     2761 ctccttgcac tgtgctcgag aaagtgttga acgagcatgt tcacccccgg ataccttgca
     2821 tgttaaaggt aacagagtgc ccataatggt tgagtataca cattcgggcg aggaggtgct
     2881 atcgtgaagg gaatcatact ggcgggcggt tccgggacga ggctctttcc cataacgagg
     2941 gtggtgagca agcaactgct ccccatctac gacaagccca tgatctacta ccccctctcg
     3001 gtgctcatgc tcgcgggcat ccgggagatc ctcgtcatct ccaccccctc ggacctgccc
     3061 aggttcatcg acctgttggg cgacggttcg cagtggggcg tctcgttcag ctacgaggag
     3121 cagccgaggc cagaagggat cgcgcaggcg ttcatcatcg gcgagcgctt cgtcggttcg
     3181 gacacggtca gtctcgtgct cggtgacaac atcttctacg gcacaggcct caaggagcgc
     3241 ctcatggggg cctcttcgat cgagcggggc agcgtggtct tcggctactg ggtgagcgat
     3301 cccgagcgct acggcgtggt gaacttcgac gaacgaggca cccccgtgga cattg
//
LOCUS       DAOSCR010000278         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18255_length_3338_cov_2.918063, whole genome shotgun sequence.
ACCESSION   DAOSCR010000278 DAOSCR010000000
VERSION     DAOSCR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18255_length_3338_cov_2.918063"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLT69_07510"
     CDS             complement(<1..655)
                     /locus_tag="PLT69_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOJ14320.1"
                     /translation="MEAAEIIRLALESGRRSLDEHEAKKVLESYGVPVVKEVVSRDPH
                     EAVAAACDIGFPVVLKGLGSKLLHKTERGLVHLGLDTPEDVIRAAEAISREASSDLEG
                     ILVQPQIKGRREFVMGITRDPIFGPVVMFGLGGVFTEAMADFSLRLAPLNQQDALAMI
                     SEIRARRLLAPFRGEAAADTDALAKALVGISRLACEHPEVGEVDVNPLLVTPEGKIVA
                     "
     gene            1255..3054
                     /locus_tag="PLT69_07515"
     CDS             1255..3054
                     /locus_tag="PLT69_07515"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOJ14321.1"
                     /translation="MKRLQERSYPKGGSERIEGRRSHGVPASPVPGDLLENILDSANF
                     GLIAADRTGRVIFVNSLARSILDIMDDLLDRRLFVGEIDPDMWESYREIMATGKPQVN
                     LPVVFRDIPLVSHRLPIVRNGEIIGAMSIFKEREAYEELANDVFRLKEYAREIEAIIE
                     SSYDGIYVTDGEANTIRVNSVYEKITGIKASEVIGRNMEELVREGYFDESVSLRVLKE
                     RRPVTIMQTLKSGKSILVTGNPIFDEGGNVRMIVTNVRDMTDLVELEQQLEYTKGMTS
                     AYRDALKSLQKANIRDNDIIMVSEPMLHIQELLERVSKTDSTVLIYGETGVGKDRIAE
                     EIHERSHRAETGIFVKINCGAIPETLLESELFGYERGAFTGARKEGKSGLFEVANRGT
                     LFLDEVESMPMVLQSKLLRVLQSFEFTRVGGTRPIKVDVRIICASNQDLKELIAKKQF
                     RADLYYRLNVIPIYVPPLRERRDDIPYLVHFFLNRFNKKHGTRKKISKEAMDHLVAYP
                     WPGNVREVANVIERLVVISHNDYIVPANLPREIFEKGVKDFFESGLTLKEQMGRIEVS
                     IIKKAIEKFGSARKAAPHLGIDASTLTRKLKKL"
BASE COUNT          663 a         1034 c         1100 g          541 t
ORIGIN      
        1 cggccacgat cttcccctcc ggggtcacga gcagggggtt cacgtccacc tcgcccacct
       61 cggggtgctc gcacgcgagc ctcgagatgc cgacgagggc cttcgcgagc gcgtcggtgt
      121 ccgcggcggc ctcacccctg aacggagcga ggagcctcct tgcgcgtatc tccgagatca
      181 tggcgagggc gtcttgctgg ttcagggggg cgagcctgag cgagaagtcg gccatggcct
      241 cggtgaagac cccgcccagg ccgaacatga cgacaggccc gaagatcggg tctctggtta
      301 tccccatgac gaactcccgc ctgcccttga tctggggctg gacgagaatg ccttccagat
      361 cggacgacgc ctcccgggag atcgcctccg ccgcgcggat tacgtcttcc ggcgtgtcca
      421 ggccgaggtg cacgagccct cgctcggtct tgtggagcaa cttggatccg aggcccttga
      481 ggacaacggg gaagccgatg tcgcatgcgg ctgcaaccgc ctcgtgaggg tccctcgaca
      541 ccacctcctt caccacgggg acgccgtagg actcgagcac cttcttggcc tcgtgctcgt
      601 ccagcgatct tctgcccgat tccagcgcga gccggatgat ctctgccgct tccatgacac
      661 ctcctctcga cctcaaggaa tggaaccccg tgcactgcgc cctgtgagag cagaccgtgc
      721 acccttgtat cactctaggc tgtgaagcga aaaagtcaac cgcacggtat cggcgcccca
      781 cgtccggcca gcctgtagag ggcacggacg gcggcggttt ctcatccaca accgcccggg
      841 ccgcacggcc cgcccgcaga gggcagggac gcacccggga ggaggccgag gaggatcggg
      901 cgagggagac ggcacatgcc aggtctgttc acgtggcgtg ccgggacgtt acagggaggc
      961 aggccgggtg aaggcttgat cgtcggtgcg ggtcttcccg gcctcctggt atgcccggta
     1021 tcgctctcat gtgcgggcgt cgtcatcagg ttgtccgggg tgggaggccc tctctcccca
     1081 cgcctgtctc ggcacccgga ggcctcctgg ccgaggtgcc ggcaggaccg acccgtgcac
     1141 agacggggca tggccggacc tgttgacaga caagcgggcg ccgtttatac cttcttcgtt
     1201 gaggaaaaga ccgaggactt gcgggcggct ccccggaagg agggtgcttc gctcatgaaa
     1261 aggctccagg aaagatcgta cccgaaaggc ggcagcgaga ggatcgaagg gcgtcgctct
     1321 cacggggtgc ctgcctcccc tgtgcccggc gatctcctcg agaacatcct cgactcggcc
     1381 aatttcgggc tcattgcggc ggacaggacg gggagggtga tcttcgtcaa cagcctggcg
     1441 cgctccattc tcgacatcat ggacgacctc cttgatcgtc ggctgttcgt gggggagatc
     1501 gacccggaca tgtgggagag ctaccgggag atcatggcca cgggcaagcc ccaggtgaac
     1561 cttcccgtcg ttttccggga catccccctc gtgagccaca ggctgccgat cgtcaggaac
     1621 ggggagatca tcggcgccat gagcatcttc aaagagcgcg aggcctacga ggagctggcc
     1681 aacgacgtct tcaggctcaa ggagtacgca agggagatcg aggcgatcat cgagtcatcc
     1741 tacgacggca tctacgtcac cgacggcgag gcgaacacca tccgggtgaa ctcggtctac
     1801 gagaagataa cggggatcaa ggcctcggag gtcatcggca ggaacatgga agagctcgtg
     1861 agggagggct acttcgacga gagcgtgagc ctcagggtct tgaaggagag aagaccggtc
     1921 actatcatgc agaccctcaa gtcgggaaag agcatcctgg tgacgggcaa ccccatcttc
     1981 gacgaaggcg ggaacgtcag gatgatcgtc acgaacgtgc gcgacatgac cgacctcgtg
     2041 gaactcgagc agcagctcga gtacaccaag gggatgacga gcgcctacag ggacgcgctc
     2101 aagtcgctcc agaaggccaa catccgcgac aacgacatca tcatggtctc cgagcccatg
     2161 ctccacatcc aggaactgct cgagagggtg tccaagacgg attcgacggt cctcatctac
     2221 ggcgagacgg gcgtcggcaa ggacaggatc gcggaggaga tccacgagag gtcccaccgc
     2281 gccgagacgg ggatcttcgt caagatcaac tgcggcgcca ttcccgagac cctcttggag
     2341 agcgagctct tcgggtacga gaggggcgcc ttcaccgggg cccgcaagga ggggaagtcc
     2401 gggctcttcg aggtggcgaa cagggggacc ctcttcttgg acgaggtgga gtcgatgccc
     2461 atggtcctcc agtcgaagct cctgcgggtg ctccagagct tcgagttcac gagggtgggg
     2521 gggacgaggc ccatcaaggt ggacgtgagg atcatctgcg cgtcgaacca ggacctcaag
     2581 gaactcatcg cgaagaaaca gttcagggcg gacctctact accgcctgaa cgtgataccc
     2641 atctacgtgc cgccgctgcg cgagaggagg gacgacatcc cctacctcgt gcatttcttc
     2701 ctgaaccggt tcaacaagaa gcacgggaca aggaagaaga tctccaagga ggccatggac
     2761 cacttggtcg cctacccgtg gccaggcaac gtccgcgagg tggcgaacgt catcgagcgc
     2821 ctcgtggtga tctcccacaa cgactacatc gttcccgcca accttccccg ggagatcttc
     2881 gagaaggggg tgaaggactt cttcgagagc ggcctcacgc tgaaggagca gatgggacgg
     2941 atcgaggtct ccatcatcaa gaaggcgatc gagaagttcg gcagcgcccg caaggccgcc
     3001 cctcacctgg ggatagacgc cagcacgctc accaggaagc tcaagaagct ctagtcgaca
     3061 cagtggccgg agcacacgcg aagacacgca cacaaacgcc gcaaggagat gccggtcgag
     3121 acgggcgtgc gcgtgcggca tcgcgggatg caggtcccgt gggccttgcc tgcctcccga
     3181 aggggatgga tcttgccgtt cgccgtgagc atgcccgatg gctcaaggtg gcgactgggg
     3241 cctgggcagg agatggacct ctccgcgcga aggcgtcgat gagcttggcg cggtgtcccg
     3301 tcgccgtcag ggttcaggag agctcgatct cccccgag
//
LOCUS       DAOSCR010000279         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18353_length_3324_cov_4.433160, whole genome shotgun sequence.
ACCESSION   DAOSCR010000279 DAOSCR010000000
VERSION     DAOSCR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18353_length_3324_cov_4.433160"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1969)
                     /gene="thrS"
                     /locus_tag="PLT69_07520"
     CDS             complement(83..1969)
                     /gene="thrS"
                     /locus_tag="PLT69_07520"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ14322.1"
                     /translation="MPQMTVTVDGKQVVTDAVTIRDLVTDRKVLAARVNGSLVDLSTP
                     VVDGMSLETVTSDTPEGLHLLRHSAAHVMAEAVKLLFPDAKPTIGPATDDGFYYDFDR
                     DEPFSEGDLARIEEKMAELVKADEPFVRREMPAEEAKEFFRAQKEPYKVEIIDDLGEG
                     VGKVSLYTQGSFTDLCRGPHVPSSAAIGAFKLLSVAGAYWRGDERNKMLQRIYGTAFP
                     TKKQLDEYLTFLEEARRRDHRKLGRELELFMFSDDVGPGMVIYMPKGGRLRAILEDFE
                     KRLHFKRGYDIVYGPAILRGRMWEISGHMDNYKENMYFTEVDGQLYGIKPMNCLSHIM
                     IYKSKVRSYRDLPLRFFELGNVTRHEKSGVIHGLLRTRQFTQDDAHIFCRPDQLIDEI
                     TSIITMVQDVMAVFGFSYTMEISTRPEKSIGSDEDWEMATRALIDSLDAKGLPYDINE
                     GDGAFYGPKIDVKIKDAIGRQWQCATIQCDFSLPTRFDTHYIGADGQRHRPVMLHRVI
                     LGSIERFIGVLIEHYAGEFPVWLAPVQAVVMNITDAQADYATKVASLLNDREIRVETD
                     LRNEKIGYKIREARAQKIPYMLVAGEKERADGTVSVRDRSGAQKTMTPEEFAGLVRSF
                     DPQL"
     gene            complement(2631..3008)
                     /locus_tag="PLT69_07525"
     CDS             complement(2631..3008)
                     /locus_tag="PLT69_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HOJ14323.1"
                     /translation="MMGIRSRLDEAHVGLKAIGSGSAEGARRFVEETGFPGRLYLSRD
                     LAAYRAFRLERGIWKTLSLASVARALEALFEGFRQGRTDGDPWQQGGVFVLGPRDVLL
                     YQYKSAFAGDLPDPDEVLEAALR"
     gene            complement(3036..3170)
                     /locus_tag="PLT69_07530"
     CDS             complement(3036..3170)
                     /locus_tag="PLT69_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14324.1"
                     /translation="MTYEELRSRVDGVMVFDLDSAVLVELCSLWEDRRTVLVFLRHFG
                     "
BASE COUNT          559 a         1142 c         1011 g          612 t
ORIGIN      
        1 ctaacctgca tcctcggcct tgtcaatacg tcagacgtcg acgaagcccc acgccgcccg
       61 gccgagaccg aggccccggc atctacagct gcgggtcgaa ggacctgacg aggccggcga
      121 actcctcagg cgtcatggtc ttctgggccc ccgagcggtc acgcaccgac acggtcccgt
      181 cggcgcgctc cttctcgccc gcgaccagca tgtaggggat tttctgggcc ctcgcctccc
      241 tgatcttgta acctatcttc tcgttcctga ggtcggtctc caccctgatc tcacggtcgt
      301 tcagcaggga ggcgaccttc gtcgcgtagt ccgcctgggc gtcggttatg ttcatgacca
      361 ccgcctggac gggggccagc cacaccggga actcgcccgc gtagtgctcg atgagaaccc
      421 cgatgaacct ctcgatggat cccaggatca ccctgtggag catgaccggg cggtggcgct
      481 ggccgtccgc accgatgtag tgcgtgtcga accgggtcgg gagcgagaag tcgcactgga
      541 tggtcgcgca ctgccactgg cggcctatgg cgtccttgat cttcacgtct atcttgggcc
      601 cgtagaatgc gccgtcgccc tcgttgatgt cgtagggcag gcccttggcg tccagggagt
      661 cgatgagcgc ccgggttgcc atctcccagt cctcgtcgct gccgatggac ttctcgggac
      721 gcgtgctgat ctccatggtg tagctgaagc cgaagaccgc catcacgtcc tgcaccatgg
      781 tgatgatcga ggtgatctcg tcgatgagct ggtcgggcct gcagaagatg tgggcgtcgt
      841 cctgggtgaa ctgcctggtg cgcagcaggc cgtggatcac gcctgacttc tcgtgcctgg
      901 tcacgttgcc gagctcgaag aaccgcaagg gcaggtcccg gtagctcctc accttggact
      961 tgtagatcat gatgtgcgag aggcagttca tgggcttgat gccgtagagc tgcccgtcca
     1021 cctcggtgaa gtacatgttc tccttgtagt tgtccatgtg cccggagatc tcccacatcc
     1081 tgcccctgag gatcgccggg ccgtagacga tgtcgtagcc cctcttgaag tgcagcctct
     1141 tctcgaagtc ctcgaggatg gccctgagcc tcccgccctt gggcatgtag atgaccatgc
     1201 ccgggccgac gtcgtccgag aacatgaaca gctcgagctc gcggccgagc tttcggtgat
     1261 ccctcctcct cgcctcctcg aggaaggtga gatactcgtc gagctgtttc ttggttggga
     1321 aggccgtccc gtagatgcgc tggagcatct tgttgcgctc gtccccccgc cagtaggcgc
     1381 ccgcgacgct caagagcttg aacgcgccta tcgcggcgct cgagggcacg tgaggcccgc
     1441 ggcacagatc ggtgaacgac ccttgcgtgt agagcgagac cttcccgaca ccctcgccca
     1501 gatcgtctat gatctccacc ttgtagggtt ctttctgcgc cctgaaaaac tccttcgcct
     1561 cctcggccgg catctccctg cgcacgaagg gctcgtcggc cttcacgagc tcggccatct
     1621 tctcctcgat cctcgccagg tcaccctcgg agaacggctc gtccctgtcg aagtcgtagt
     1681 agaagccgtc gtcggtcgca ggcccgatcg tgggcttcgc gtccgggaac agcagtttca
     1741 ccgcctccgc catgacgtgg gcggccgaat gcctgagcag gtgcaggccc tcgggcgtgt
     1801 cggacgtcac ggtctccagg gacatgccgt ccacgaccgg cgtcgacaga tcgacgagcg
     1861 acccgttcac tcgtgcggcg agcaccttcc tgtcggtgac gagatcccga atggtaacag
     1921 catccgtcac cacctgcttt ccatcaacag tcacggtcat ctggggcata tgcactccct
     1981 gcggctttct cggccaggtt ccctgagacc gagggcggcc gcgcacttgg tcgtgggcga
     2041 cccggtacag acacgtgccg ccgtcgtctc cagacaggac tgataccacc tggcaggcct
     2101 tttgtaaagc aaggcccttg ccggcgcgga catcccccca ggcggcaatg aggccgggtc
     2161 aagaggggca ccgggcacgg ccaggcctac ccgatgccgc ccgtgtcgtt ccccgggagc
     2221 gctcgaaggc tcggcctcgg gcatgggacg cgcccgatca tgtcactggc cgcaagcacc
     2281 accggcggtg ctctgaagaa ccagctgcgc aaacccttga gcgcaccgtg gatctggcgc
     2341 ccccggcaat tccatggtcg ggcgtccctc cagggagagg gccttcacca gtcacgccgc
     2401 ccctggcagg gccatggggc cagtcgactt gcatgcccgg ggccggggac gacccctcgg
     2461 ccttctgcat ggagtcgccc atggcgatgc aggtctcggc gacgaccccc gcccccccgg
     2521 cgtcctgaac agggcgatgc ttgaccaaac gtctcccatg gcatcctaca ggcagacacg
     2581 ggccgggccc gccgacaagt cctcgaaggt gcggccgtga gggggcgcac ctacctcaag
     2641 gccgcttcca gcacctcgtc cggatccggg aggtcgcccg cgaacgcgct tttgtactgg
     2701 tacaagagca cgtccctggg gcccaggaca aagacacccc cctgttgcca ggggtcgccg
     2761 tcggtccttc cctggcggaa gccctcgaac agagcctcga gggcccttgc cacggaggcc
     2821 agagagagcg tcttccatat tccccgctcg agcctgaacg cccggtatgc cgcaaggtcc
     2881 ctgctcaggt acagcctccc cgggaaaccc gtctcctcga cgaaacgcct cgccccttcg
     2941 gcgctcccgc tccctatggc cttgagcccg acatgtgctt cgtccagcct cgatcgtatt
     3001 cccatcatgg cggcgacctg ccgcctcgcg aatattcaac cgaagtggcg caggaacacg
     3061 agcaccgtgc gccggtcttc ccagagcgag cacagctcga ccagaacggc cgagtcgaga
     3121 tcaaagacca tgaccccatc caccctcgac ctcagctctt cgtaggtcat gagcaccccc
     3181 tgtctgcttg cctgaaccac cgcgtcttgg tacgatcacc cgtcgagagg aacaagatac
     3241 ccccggggaa gcgaaaggca aggccgcctt ggcggcagga ggtgcgcatg acgatgacga
     3301 gggaacaggc gggtgagttc gcat
//
LOCUS       DAOSCR010000280         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18424_length_3315_cov_4.477607, whole genome shotgun sequence.
ACCESSION   DAOSCR010000280 DAOSCR010000000
VERSION     DAOSCR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18424_length_3315_cov_4.477607"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLT69_07535"
     CDS             complement(<1..405)
                     /locus_tag="PLT69_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14325.1"
                     /translation="MERLAAFIYRLRAWLFSAVILAGVFFAANIRFEMNNKLDAWFED
                     DDPVSVDFRLFRDTFEGGDSLIVGLESENLFTRENLAYLVAKTDELETVDHVRYVVSL
                     ANANRVRGTEQGIDVRPLLEGYERTPPREIRRA"
     gene            complement(422..1606)
                     /gene="tyrS"
                     /locus_tag="PLT69_07540"
     CDS             complement(422..1606)
                     /gene="tyrS"
                     /locus_tag="PLT69_07540"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ14326.1"
                     /translation="MLEQIAELKRGIVDIVSEGELLEKLKRGRPLRVKVGFDPTAPDI
                     HLGHTVVLQKMRQFQDAGHDVICLIGDFTGMIGDPSGRSTTRPPLSRQEVLENAKTYQ
                     EQVKKILDPKRTIVRFNSEWMDTMVVADFIKLTSVQTVARMLERDDFKKRFQSQHPIS
                     IHEFLYPFVQGYDSVALEADVELGGTDQIFNLLMGREVQKAYGQEPQVVMTLPLLEGT
                     DGVLKMSKSYNNYIGVDEPPRQMYGKVMSISDGLMWKYYELLSTLRLGEIEELRKAVE
                     GGSLHPMDAKMRLAFEIVERFHGREEAGRAQEEFIRVFRAKEIPEDLPEVALVLERPW
                     ICAVLKEAGLVSGTSEARRMIAQGAVHVDDARVTDAELVLDKGVHIIKVGKRRFAKVA
                     IG"
     gene            complement(1710..3266)
                     /gene="rny"
                     /locus_tag="PLT69_07545"
     CDS             complement(1710..3266)
                     /gene="rny"
                     /locus_tag="PLT69_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOJ14327.1"
                     /translation="MTMTIVSIAVALVVGALLGIGLNALVRGKTLSDARAEGARIIDE
                     AQKEAANIEAEAQLKAKEIVFTAKTQSDAEAKERLKEVSVIEKRIHQREELIDNKLSQ
                     LDKREQEIRKAEQSLSDRMRHVENLKAEVESLRKQQIDKLEEISGMDSNQAKKELMAS
                     LESEARHEAAKLLKQIQDEAEAEADKRAKRILSTAIQRYSTDVVSTRTVSVVNLPSEE
                     MKGRIIGREGRNIRSIEAATGVDLIIDDTPDAVILSSFDPIKREIARLSLETLVVDGR
                     IHPSRIEEVVEKVTKEINEAIWEAGQQATFELGLHGLNPELVKLVGKLKYRTSFSQNV
                     YQHSLEVAHLCGILAAELGVNQKIAKRVGLLHDIGKAVDHEVEGSHAIIGADMAKRYG
                     ESNKVVEAIASHHNEAPVNSVYGFLVQAADSLSAARPGARREMLENYIKRLVELETIA
                     TSFDGVSKAYAVQAGREVRVMVDVDKVSDAGADLLAQQITKAIEEKLTYPGQIRVVVI
                     RESRAVRFAK"
BASE COUNT          494 a         1134 c          963 g          724 t
ORIGIN      
        1 cgcgcgcctg atctcccgcg gtggcgtgcg ttcgtatccc tccaggaggg gcctcacgtc
       61 gataccctgc tcggtgcccc tcacccggtt cgcattggcc aggctcacca cgtacctgac
      121 atggtcgacg gtctcgagtt cgtcggtctt ggcgaccagg taggcgaggt tttccctcgt
      181 gaagaggttt tcggactcga gaccgacgat gagggagtct cccccctcga aggtgtccct
      241 gaagagccgg aagtccacgg agaccgggtc gtcatcctcg aaccaggcgt cgagcttgtt
      301 gttcatctcg aacctgatgt tcgccgcgaa gaacaccccg gcgaggatca cggcggaaaa
      361 gagccacgcc ctcaaccggt agatgaatgc ggccaggcgc tccatggcgg cggggagggc
      421 ctcagcctat cgcgaccttt gcgaacctgc gcttgcccac cttgatgata tggactccct
      481 tgtcgagcac gagctcggcg tccgtcaccc gcgcatcgtc cacgtgaacc gccccctggg
      541 cgatcatgcg tctcgcctcg gacgtgccgg acacgagccc ggcctccttg aggaccgcgc
      601 atatccaggg cctctcgagc acgagggcga cctccgggag gtcttcggga atctccttgg
      661 ccctgaacac ccgtatgaac tcctcctggg cacgcccggc ctcttcacgc ccgtggaagc
      721 gctccacgat ctcgaaggcc aggcgcatct tcgcgtccat ggggtggagg gacccgccct
      781 ccacggcctt cctgagctcc tcgatctccc cgagccgcaa ggtgctgagc agctcgtagt
      841 acttccacat gagcccgtcc gagatggaca tgaccttgcc gtacatctgg cggggcggct
      901 cgtccacccc gatgtagttg ttgtatgact tgctcatctt caggacgccg tcggtgccct
      961 ccaagagcgg cagcgtcatc accacctggg gctcctggcc gtaggccttc tggacctccc
     1021 tgcccatgag caggttgaag atctggtccg tgccgccgag ctccacgtcg gcctcgaggg
     1081 ccacggagtc atagccctgc acgaacgggt agagaaactc gtggatgctg atcgggtgct
     1141 gcgactggaa gcgcttcttg aagtcgtcgc gctcgagcat cctcgcaacg gtctgaacgg
     1201 aggtgagctt gatgaagtcc gcgaccacca tggtgtccat ccactctgag ttgaaccgca
     1261 cgatggtcct cttcgggtcg aggatcttct tcacctgctc ctggtaggtc ttcgcgttct
     1321 cgaggacctc ctgcctggag agcggcggcc tcgtcgtgct cctcccgctc ggatccccga
     1381 tcatgcccgt gaaatcgcct atgaggcaga tgacgtcgtg acccgcatcc tggaactggc
     1441 gcatcttctg gaggaccacc gtgtggccca ggtggatgtc gggcgccgtc gggtcgaacc
     1501 ctaccttcac cctgagcggc ctgcccctct tcagtttctc aaggagctcc ccttccgaga
     1561 cgatgtccac gataccgcgt ttcaactccg caatctgttc cagcatcatc cgcccctttc
     1621 cacgagactg tgcccaccat cgagggggac cgcccgccgt catgccggca taccggccgc
     1681 ctcgggccga gctctctgcc cgccctcgtt cacttcgcga atctcactgc cctgctctcg
     1741 cgtatcacca ccacacggat ctggccggga taggtcagct tttcctcgat ggccttggtg
     1801 atctgttgcg ccaggaggtc ggcgccggca tcgctgacct tgtccacgtc gaccatgacg
     1861 cggacctccc tgccggcttg aacggcgtac gccttcgaga cgccgtcgaa ggaggtggct
     1921 atggtctcga gctcgacgag cctcttgatg tagttctcga gcatctccct cctggccccg
     1981 ggcctggccg cggagagcga gtccgcagcc tggacgagga agccgtagac cgagttcacc
     2041 ggtgcctcgt tgtggtggga ggctatggcc tccacgacct tgttggactc gccgtagcgc
     2101 ttcgccatgt ccgccccgat gatcgcgtgc gatccctcga cctcgtggtc cacggccttg
     2161 cctatgtcgt ggagcagccc cacgcgcttg gcgatcttct gattcacgcc gagctcggcc
     2221 gcgaggatgc cgcagaggtg ggcgacctcc agcgaatgct ggtagacgtt ctgggagaag
     2281 ctcgtgcggt atttgagctt tccgacgagc ttgacgagct cggggttgag cccgtgaaga
     2341 ccgagctcga aggtcgcctg ctgacccgcc tcccatatgg cctcgttgat ctccttggtc
     2401 accttctcca cgacctcttc gatcctcgag gggtggatcc tcccgtccac cacgagggtc
     2461 tcgagggaga gtctcgctat ctcgcgcttg atgggatcga agctcgagag gatgacggca
     2521 tccggcgtgt cgtcgatgat gagatccacg cctgtcgccg cctctatcga gcggatgttg
     2581 cggccttccc tcccgatgat gcggcctttc atctcctcgc tcgggaggtt caccaccgag
     2641 accgtcctcg tgctcaccac gtccgtcgag tatctctgga tggccgtgga caggatcctc
     2701 tttgcgcgct tgtccgcctc cgcctccgcc tcgtcctgga tctgcttgag gagcttcgcc
     2761 gcttcgtggc gcgcctcgct ctccagggag gccatgagct ccttcttcgc ctggttcgag
     2821 tccatgccgc tgatctcctc gagcttgtcg atctgctgct ttctcagcga ctccacctca
     2881 gccttgaggt tctccacgtg gcgcatcctg tccgacagcg actgttccgc cttccggatc
     2941 tcctgctcgc gcttgtccag ctggctgagc ttgttgtcta tgagctcctc gcgctggtgt
     3001 atcctcttct ctatgacgga gacctccttg aggcgctcct tcgcctcggc gtcgctctgc
     3061 gtcttcgccg tgaagacgat ctccttggcc ttgagctggg cctccgcctc tatgttcgca
     3121 gcctccttct gcgcttcgtc tatgatgcgg gcaccctcgg cccttgcatc gctcagcgtc
     3181 ttgcccctga cgagggcgtt caacccgata cccaggagtg cacccactac gagtgcaacc
     3241 gctatggaga cgattgtcat tgtcattcct cgccttcttt atgtattccg gcctgggtcg
     3301 cgacgtagcc gaccc
//
LOCUS       DAOSCR010000281         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18470_length_3310_cov_2.539785, whole genome shotgun sequence.
ACCESSION   DAOSCR010000281 DAOSCR010000000
VERSION     DAOSCR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18470_length_3310_cov_2.539785"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /gene="glgP"
                     /locus_tag="PLT69_07550"
     CDS             complement(<1..1824)
                     /gene="glgP"
                     /locus_tag="PLT69_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ14328.1"
                     /translation="MKVKSFRVVPTLPETLGPILKLAYNTWYCWNHKGLRLFQHMDRD
                     LWEETGHNPVDMLSRISQGRIMDLLEDEGFLSQMKKTVEEFDDYMEEKGVYSFLLAKP
                     VDFTVAYFSMEYGLTESIPIYSGGLGILAGDHLKSASDLRLPLCGVGLLYRHGYFTQY
                     LSWDGWQQEESTNNDFYHMSLVLEQDKDGKPLKVSVRLAERECIAQVWRCQVGRVPLY
                     LLDTNIMENHPDERNVTGGLYAGNIEDRLRQEIVLGIGGVRALTALGLDPMIYHMNEG
                     HCFLVALERTRALMKKHGLDFKTARELVRASLVFTTHTPVPAGNDVFDIGLVESYLRP
                     YVDEIGVPWQEFVALGRKNPRDEGEPFGATVFAIKNSAFRNGVSKLHGEVSRDMWRDI
                     WPHVPREDVPISSITNGIHIPSWISDEMAELFDRYLGPKWKEDPDNQKVWERIDEIPD
                     AELWATHQRRRERLVAFARRRLHRQLASRGAKTKDLQRAMETLHPDALTIGYARRFAE
                     YKRGLLIFRDIERLKAILSGRERPVQLIISGKAHPRDNEGKAIIQRIIHISREEPFKD
                     RVVFLEDYNMNITRCLVEGVDVWLNNPRRPMEACGTSGMKAT"
     gene            complement(1942..2691)
                     /gene="ybgF"
                     /locus_tag="PLT69_07555"
     CDS             complement(1942..2691)
                     /gene="ybgF"
                     /locus_tag="PLT69_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOJ14329.1"
                     /translation="MNRLLVAAIVALAVAGCATQRDLDNLKFQVDTLQSRLSAAEARL
                     GEKEKLIDQSLRQQGELQNRYSELQNQIFSLEGSLDEVKVSAGLTPAGGGESRISALE
                     KEVRALKEMVQGGAAVQQQGAARSLFDTGMEKFTSGKFQEALADFKAFLGKNPEQSLA
                     GEAYFFMGEALFSLGRYDDAVLSYDTVVKKFKNSGRIPDALYKEGLAFEKMGDRETGE
                     IIWQQLLKEYPKTEAASKAREAMKARKPGKG"
     gene            complement(2694..3287)
                     /gene="pal"
                     /locus_tag="PLT69_07560"
     CDS             complement(2694..3287)
                     /gene="pal"
                     /locus_tag="PLT69_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOJ14330.1"
                     /translation="MNEKRNGKRTAAGTVILGILAALAATLLLTGCPKKAVAPVAPET
                     PSAVQPQAPEEAPGEPVMRVEPALEGAGRAAGTTSAQAVSAIEETDIFFEFDSFDLSP
                     TAKKTLADKAAYLKAHQGIKVQIEGHCDERGTVEYNLALGERRAKAAYEYLVFLGVDP
                     KRLSIVSYGEERPVDPGHNEEAWAKNRRAHFAVSQGY"
BASE COUNT          483 a         1155 c         1004 g          668 t
ORIGIN      
        1 cgtcgccttc atccccgagg tgccgcaggc ctccatgggg cggcgggggt tgttgagcca
       61 cacgtccacg ccctcgacga ggcagcgcgt gatgttcatg ttgtagtcct ccaggaacac
      121 caccctgtcc ttgaacggct cctcgcggga gatgtggatg atccgctgga tgatggcctt
      181 gccctcgttg tcgcgggggt gggccttgcc ggatatgatg agctggacag ggcgttccct
      241 gccggagagg atcgccttga gcctctctat gtccctgaag atgagcagcc ccctcttgta
      301 ctcggcgaac ctcctcgcgt acccgatggt gagcgcgtcc gggtgcagtg tctccatggc
      361 gcgctggagg tccttggtct tcgcgccccg cgaggcgagc tgccggtgga gccgcctgcg
      421 ggcgaaggcc acgagccgct ccctgcgcct ctggtgggtg gcccagagct cggcgtcggg
      481 gatctcgtcg atccgctccc acaccttctg gttgtccggg tcttccttcc acttcgggcc
      541 gaggtaccgg tcgaagagct ccgccatctc gtccgagatc caggatggta tgtggatccc
      601 gttggtgatg gaggatatgg ggacgtcctc cctcgggacg tgcggccaga tgtccctcca
      661 catgtccctg gagacctctc cgtggagctt gctcaccccg ttccgaaacg ccgagttctt
      721 gatcgcgaac acggtggcgc cgaacggctc cccctcgtcc ctggggttct tcctgcccag
      781 cgcgacgaac tcctgccacg gcaccccgat ctcgtcgaca tagggcctga gatagctctc
      841 cacgagcccg atgtcgaaca cgtcgtttcc ggcgggcaca ggcgtgtggg tcgtgaacac
      901 gagggacgcc ctgacgagct ccctggcggt cttgaagtcg agcccgtgct tcttcatgag
      961 ggccctggtg cgctcaagcg ccacgaggaa acagtgcccc tcgttcatgt ggtagatcat
     1021 ggggtcgaga ccgagggccg tgagcgcccg cacccccccg atgcccagca cgatctcctg
     1081 cctcagcctg tcctctatgt tgcctgcata caggccgccc gtgacgttcc tctcgtccgg
     1141 gtggttctcc atgatattcg tgtccaggag gtagaggggc acgcgcccca cctggcagcg
     1201 ccagacctgg gcgatgcact ccctctccgc gagcctcacc gagaccttga gcggcttgcc
     1261 gtccttgtcc tgctcgagca cgaggctcat gtggtagaag tcgttgttgg tcgactcctc
     1321 ctgctgccac ccgtcccagc tcaggtactg ggtgaagtag ccgtgcctgt agagcagccc
     1381 caccccgcac aggggaagcc tcaggtcgct cgcgctcttg aggtggtcgc ctgccaggat
     1441 gccgagcccc cccgagtaga tggggatgct ctcggtcagg ccgtactcca tggagaagta
     1501 cgcgacggtg aagtcgacgg gcttggccag gaggaacgag tacacgccct tctcttccat
     1561 gtagtcgtcg aactcctcga ccgtcttctt catctgcgag agaaagccct cgtcctcgag
     1621 gaggtccatg atgcggccct gggatattct cgacagcatg tccacggggt tgtgcccggt
     1681 ctcctcccac aggtcgcggt ccatgtgctg gaagagacgc aaccccttgt gattccagca
     1741 gtaccacgtg ttgtacgcga gcttgaggat cggacccagc gtctcaggca gggtgggcac
     1801 caccctgaag gacttgacct tcatgaaacc ccctttcttc ccttctcaac gtcacccggg
     1861 gaaccctccc gcgggcgaca ctccaaccca acgccacggc gcacgcaggg cgccccttca
     1921 cctccggcgc cttgaggcgg cctacccctt gccgggcttc ctggccttca ttgcctccct
     1981 cgccttcgat gcggcctccg tcttcggata ctccttgagg agctgctgcc agatgatctc
     2041 gcccgtctcc ctgtcgccca tcttttcgaa ggcgaggccc tccttgtaca atgcatcggg
     2101 aatcctgccg ctgttcttga acttcttcac cacggtgtcg tagctcaaga ccgcgtcgtc
     2161 gtacctgccg agcgagaaca gcgcctcccc catgaagaag taggcctctc ccgccaggga
     2221 ctgctcgggg ttcttgccca ggaatgcctt gaaatcggcc agcgcctcct ggaatttccc
     2281 cgaggtgaac ttctccatgc ccgtgtcgaa gaggctcctg gcggccccct gctgctggac
     2341 cgccgcgccg ccctggacca tctccttgag cgcacgcacc tccttctcga gcgccgagat
     2401 cctggactcg ccgccgcctg cgggcgtcag ccccgcggag accttcacct cgtccaggga
     2461 accctcgagc gagaagatct ggttctggag ctcggagtac ctgttctgca gctcgccctg
     2521 ctgcctgagg ctctggtcga tcagtttctc cttctcgccg agcctggcct cggccgcgct
     2581 caggcgcgac tggagcgtgt ccacctggaa cttcaggttg tcgaggtcgc gctgcgtggc
     2641 gcagcccgct acagcgagcg caacgatcgc cgcaaccaag agccgattca tggtcaatac
     2701 ccctggctga ccgcgaagtg ggcgcgcctg ttcttcgccc aggcctcctc gttgtgcccc
     2761 ggatcgaccg gcctctcctc gccgtagctg acgatggaga gcctcttcgg gtcgaccccg
     2821 agaaagacga ggtactcgta ggccgccttc gcgcgcctct ctcccagggc gaggttgtac
     2881 tccacggtgc ccctctcgtc gcagtggccc tcgatctgca ccttgatacc ctggtgggcc
     2941 ttcaggtagg cggccttgtc cgcgagcgtc ttcttggccg tgggtgagag gtcgaagctg
     3001 tcgaactcga agaagatgtc ggtctcctcg atggcggaga cggcctgggc gctcgtggtc
     3061 ccggccgccc tgcccgcgcc ctcgagcgcc ggctccaccc gcatgaccgg ctcgcccggg
     3121 gcctcctcgg gggcctgcgg ctgcacggcg gagggcgtct ccggggcgac gggcgccacc
     3181 gccttcttgg ggcaccccgt gagcagaagc gttgcggcaa gcgccgccag gatgccaagg
     3241 atcaccgttc ccgccgccgt cctcttcccg ttccttttct cgttcatatc cgtgttcctc
     3301 cccctcgatg
//
LOCUS       DAOSCR010000282         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18474_length_3309_cov_2.671481, whole genome shotgun sequence.
ACCESSION   DAOSCR010000282 DAOSCR010000000
VERSION     DAOSCR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18474_length_3309_cov_2.671481"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLT69_07565"
     CDS             <1..645
                     /locus_tag="PLT69_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ14331.1"
                     /translation="IQTCFLRPAKDPTVLGLILITLGAAMLLMGLAGWQWGAEQKDFP
                     FILSVQKTREIAPGLELNEWSMGVFVLSAVIMLILFLFFRYSRLGIAMRATQQNVNAA
                     KVMGIKVERVFAITWAMSSVIGAVAAMFFAARSVLDPNFMMEPFMRAFAAAVLGGLTS
                     IPGVVAGGLIMGIVENVFGYRWPEWKPIVAFVVIVLILTLKPSGLFAKHFVKKV"
     gene            649..1119
                     /locus_tag="PLT69_07570"
     CDS             649..1119
                     /locus_tag="PLT69_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14332.1"
                     /translation="MGAFLPMGRAKTAAVLAVMASCTAVLVAWDSPRMRIELILERPS
                     IERAALEYLEAEMSGDHAKVYSMLAPSSAYVRSHTYGQYLEEASGSTVKVRGYRIVDI
                     YRLRPNHDPNSFPGVERFVQVEVEVDVGFDDMETTTTCNYCFTFLKEKGRWYKG"
     gene            1341..2534
                     /locus_tag="PLT69_07575"
     CDS             1341..2534
                     /locus_tag="PLT69_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ14333.1"
                     /translation="MRRTVLGMFVFAVACAILASWPAHAARPGFDAKEIRIAQWGPQT
                     GPAAPWGAVARGSALLFNMVNDSGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGI
                     FAFVGGVGAACGMAVKDYLAQNKVIWVSPSTAIDGYVFPVNPYLFAVYPLYDDEASIL
                     TKYIVEKLGAKKIAFFYQNDAYGKSGLDGCKTRMDALKMKLVEEIPVEPGEKDLSSQM
                     LRLKNSGAEVVIMWVNPTTAAIALKTSQTIGFKPQWVSSNTLSDYPLMNKITGGLWEG
                     VITGAFGVPPTADHPLMNKYREAAKKYAPEERFGTFYLAGVLFAEPLVEALKRTGPNL
                     STDACLKQLNSIRNFQGIGPVINWSPTVHQGTDSIQIQKCGPDSSYILLQDWTSNDLA
                     RWKKK"
     gene            2646..>3309
                     /locus_tag="PLT69_07580"
     CDS             2646..>3309
                     /locus_tag="PLT69_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ14334.1"
                     /translation="MAHVRVENLSISFGGIKAVDDVSFEVEDNTIFSIIGPNGSGKTT
                     IFNMISGIYRPDSGRVTLDGEDLVGKRPDKIAKKGIARTFQNIQLFTNATVMDNIMLG
                     RHIHMKTGVLGGAFMWGRSSPTAKEEVKNREIVEHIIDFLDLQSVRDQFVGNLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYRVTILFIEHDMNMV
                     MGI"
BASE COUNT          655 a         1024 c         1099 g          531 t
ORIGIN      
        1 atccagacgt gcttcctgag gccggccaag gaccccacgg tcctcggtct catcctcatc
       61 accctcggcg cggcgatgct cctcatgggg ctcgccgggt ggcagtgggg ggcggagcag
      121 aaggacttcc cgttcatcct ctccgttcag aagacgaggg agatcgcccc gggcctcgag
      181 ctgaacgagt ggtccatggg cgtgttcgtg ctctcggcgg tgatcatgct gatcctcttc
      241 ttgttcttca ggtactcgag gctcggcatc gcgatgcggg cgacccagca gaacgtgaac
      301 gccgccaagg tgatggggat caaggtcgag cgggttttcg cgatcacgtg ggccatgagc
      361 tcggtcatcg gtgcggtggc cgccatgttc ttcgcggcca ggagcgtcct cgaccccaac
      421 ttcatgatgg agcccttcat gcgtgccttc gctgcggccg tgctcggggg cctcaccagc
      481 atccccgggg tcgttgcagg gggcctcatc atgggcatcg tggagaacgt cttcggctac
      541 aggtggcccg agtggaaacc gatcgtggcc ttcgtggtca tcgtgctcat cctgacgctc
      601 aagccgagcg ggctgttcgc caaacacttc gtgaaaaagg tgtgagacgt gggtgcattc
      661 cttcccatgg gcagggcaaa gaccgcggcc gtcctcgcgg tcatggcttc ctgcaccgcg
      721 gtgctcgtcg cgtgggactc cccgcgcatg cgcatcgagc tgatcctcga gaggccctcc
      781 atcgagcgcg ccgccctcga gtacctcgag gcggagatga gcggggacca cgcgaaggtc
      841 tactcgatgc tcgccccctc gtccgcatac gtgcgcagcc acacctacgg gcagtacctc
      901 gaggaggcat ccggctccac cgtgaaggtc aggggatacc gcatcgtgga catataccgc
      961 ctgaggccga accacgaccc gaattccttc ccgggggtcg agaggttcgt ccaggtggag
     1021 gtcgaggtgg acgtggggtt cgacgacatg gagaccacga ccacctgcaa ctactgcttc
     1081 acgttcctca aggagaaagg ccgctggtac aaggggtgaa cgagcaccag ggcggcgggg
     1141 ggtcgcgggc gcgggccgga tgagcgcgcc cggccgggaa gaaaggggct gtcgggccga
     1201 accccggcac gggatacggg gcaaagggcg ccgcatccat cgtggtcgac gagggctgaa
     1261 cgccaggggt accggctgaa gcggcagcgg cctgcgggta cggccgctgc acggtacaac
     1321 ctgaacagga aggaggacgg atgaggagaa cagtgctcgg catgttcgtg ttcgcggtcg
     1381 catgtgcgat cctggcttca tggccggccc atgcggcaag gccaggtttc gacgcaaagg
     1441 agatacgcat cgcccagtgg ggcccgcaga cggggcctgc ggcaccctgg ggtgcggtgg
     1501 ccagggggag cgccctgctc ttcaacatgg tcaacgactc cggcggcatc cacgggagga
     1561 agatcaagta tttcatccgc gacgatcagt acaacccggc gcagacgaag gccgtggtca
     1621 aggagctcgt ggaacgcgag ggcatcttcg ccttcgtggg cggggtgggc gccgcctgcg
     1681 gcatggcggt gaaggactac ctggcccaga acaaggtcat ctgggtgagc ccgtcgaccg
     1741 ccatcgacgg atacgtgttc ccggtgaatc cctacctctt cgccgtgtac cccctgtacg
     1801 acgacgaggc gagcatcctg acgaagtaca tcgtggagaa gctcggcgcg aagaagatcg
     1861 ccttcttcta ccagaacgac gcctacggca agagcggcct cgacgggtgc aagacccgca
     1921 tggatgcgct caagatgaag ctcgtggaag agatcccggt ggagcccggc gagaaggacc
     1981 tctcgtcgca gatgctcagg ctcaagaact cgggggctga agtggtcatc atgtgggtga
     2041 accccaccac cgccgccatc gccctcaaga cgagccagac catcggcttc aagccgcagt
     2101 gggtatcctc caacaccctg tccgactacc cgctcatgaa caagatcacg ggcggcctct
     2161 gggaaggggt catcacgggc gccttcggcg tcccgccgac tgcggaccac ccgctcatga
     2221 acaagtatcg ggaggcggcc aagaagtacg cgcccgagga gcgcttcggc accttctacc
     2281 tggcgggcgt gctcttcgcc gagccgctgg tggaggccct caagaggaca ggccccaacc
     2341 tgagcaccga tgcgtgcctg aagcagctca actccatacg gaacttccag gggatcggcc
     2401 cggtgatcaa ctggtcgccg accgtccacc agggaacgga ctcgatccag atccagaagt
     2461 gcgggcccga ctcgagctac atcctgctgc aggactggac gagcaacgac ctggccaggt
     2521 ggaagaagaa gtgatccttc aaggggcggg ccccgcccgc ccccggttcg cacagggggc
     2581 gcacacccgc gggtgggccg ataccgtgtc gggcgagaga cggaccgctt gagaggcatg
     2641 cgagcatggc gcacgtacgt gtcgagaact tgagcatcag cttcggcggc atcaaggccg
     2701 tggacgacgt gagcttcgag gtggaggaca acacgatctt ctccatcatc gggcccaacg
     2761 gcagcggcaa gaccaccatc ttcaacatga tcagcggcat ctacaggcct gactccggca
     2821 gggtgacgct cgacggcgag gatctcgtgg gcaaaaggcc ggacaagatc gcgaagaagg
     2881 gcatcgccag gacgttccag aacatccagc tcttcaccaa cgccacggtc atggacaaca
     2941 tcatgctcgg caggcacatc cacatgaaga ccggcgtgct cggcggcgcc ttcatgtggg
     3001 gcaggagctc gcccaccgcg aaggaagagg tgaagaaccg cgagatcgtg gagcacatca
     3061 tcgacttttt ggacctccag tccgtgcgcg accagttcgt gggcaacctc ccctacggaa
     3121 agaggaagct cgtcgagctc ggcagggcgc tcgcgctcga gccgaaggtg ctgctcttgg
     3181 acgagccctc cgcgggcatg aacaccgagg aaaaggacga cctgcgcatc tggatcaagg
     3241 acatccagga ggactaccgg gtgaccatcc tgttcatcga acacgacatg aacatggtca
     3301 tggggatct
//
LOCUS       DAOSCR010000283         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18630_length_3288_cov_3.575317, whole genome shotgun sequence.
ACCESSION   DAOSCR010000283 DAOSCR010000000
VERSION     DAOSCR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18630_length_3288_cov_3.575317"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLT69_07585"
     CDS             complement(<1..448)
                     /locus_tag="PLT69_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOJ14335.1"
                     /translation="MKIRISKEYVLLALLIASSVAYLVYKKADRVHYRLPALEPVKQE
                     DITSMTVEKGDGKIVLEKAGTDWVISPAGWKADRVKVDEMREMLSTLVITELVSESGD
                     YGRYQLDEKSRARIRAYAGDRLVRDLTAGKAAPTYNHTYVLLPGDRN"
     gene            complement(445..2607)
                     /locus_tag="PLT69_07590"
     CDS             complement(445..2607)
                     /locus_tag="PLT69_07590"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOJ14336.1"
                     /translation="MRAQRHGRIVKFLVYLALVILINAAASTLFLRFDLTANRMYSLS
                     EVSKTVVGSLKEPMTINVFFTPDLPAPYNTVEQYLRDLLSEYALYANKSFNYRFYRVG
                     EEDAQQPDVGVDTRRLASDYGITPVQIQVVEKDEVKFKRAYMGLAIVHGDMIERIPAL
                     TTTEGLEYKLTTSMQKLANKISVLSGLKDKVEVKLYLSSSLEAVSSRMSLKDLDRVPA
                     QVEKAVGDLNARLFNAISYRRVDLDGRQAMEEASRTYNLMMLTWPDLDGGRVKAGAGL
                     AGMVVEYGGRSYTIPVIQAYTIPLFGTQYRLTPVEKMAEALEQSIESLVGINETLGYL
                     ADKGTLGLYGQPGSSASLNNFQDLASRVYTIKEITLDKGIPEGLGCLLIVRPTEAFTD
                     YELFQIDQALMRGTNIAVLLDPFVERPADPNQPMQPGQLMPVTTGIERLLAHYGVKVE
                     MSLVCDLNSFKQRMPEQYGGGEQAIYYAPLIKPSQINHEPRYMKNINELITYKIAPLS
                     LDTTRMKENNLEATVLFTTSDKAWELKNPTMLNPMFVREPGDPSSMGTRTLACLVQGA
                     FPSYFAGKDIPAAPPAHQGEGASGAQEGGKAASPALGHGGAVLERSRPAKLLVIGSSE
                     MITDNLVTEEGTNPNAVFLMNLIDVLNNREEIAVMRAKTQTINPLEVTDENVRLAAKA
                     FNVLGLPVLVILSGIVVWAARAARKKRIDAMFSRRADA"
     gene            complement(2611..>3288)
                     /locus_tag="PLT69_07595"
     CDS             complement(2611..>3288)
                     /locus_tag="PLT69_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ14337.1"
                     /translation="REFMDYFTTPIAYIVICAFLVVTGWFFFATFFIMNQTSLRTFFS
                     MLPLVFPFVVPAVTMRLFAEELHSGSLEMLLTLPMDLSDAVIGKFLASSAFVAVMLAP
                     TLSYGIWVSFMGGLDWGPVAGGYAGAVLLGAAYCAIGVFASTLTRNQIISFILGMVIC
                     LALSLVDKMLFFFPPDVLAVLHYLGASSHFENIAKGVVDTRDIVYFASVVFLSLYGAH
                     LVMQEKR"
BASE COUNT          572 a          988 c         1070 g          658 t
ORIGIN      
        1 tgttcctgtc gccaggcagc agcacatagg tgtggttgta cgtgggagcg gccttccccg
       61 ccgtcaggtc gcggaccagc ctgtcgccgg cgtatgcgcg tatgcgggcc cgggacttct
      121 cgtcgagctg gtaccttccg taatcaccgg actcggagac gagctcggtg atgacgagcg
      181 tggagagcat ctccctcatc tcgtccacct tcacccggtc ggccttccag cctgcggggg
      241 agatcaccca gtccgtcccg gccttctcga ggacgatctt cccgtcgccc ttctcgacgg
      301 tcatggaggt gatgtcttcc tgcttcaccg gctcgagcgc cgggaggcgg tagtggacac
      361 ggtccgcctt cttgtagacg agataggcga cggaagatgc gatgagcaag gccagaagga
      421 catactcctt ggagatcctg atcttcatgc gtcggccctc ctggagaaca tggcgtcgat
      481 gcgcttcttc ctggcggctc tcgcggccca cacgacgatg cccgagagga tgaccagcac
      541 aggcagcccg agcacgttga acgccttggc cgccagcctc acgttctcgt cggtcacctc
      601 gagcgggttg atggtctggg tcttggcgcg catcacggcg atctcctccc tgttgttcag
      661 gacatcgatc aggttcatga ggaacacggc gttggggttc gtcccttctt ccgtgacgag
      721 gttgtccgtg atcatctccg atgaccctat caccaggagc ttggcgggcc tggatctctc
      781 gaggaccgcc ccgccgtggc ccagggcggg tgatgcggcc ttgccgcctt cctgcgcgcc
      841 cgaagcgcct tccccctggt gggccggcgg tgcggccggg atgtccttcc ccgcgaagta
      901 gctcgggaaa gcgccctgga cgaggcacgc aagggtcctg gtccccatgg aggacgggtc
      961 gccgggttcc cttacgaaca tggggttgag catcgtcggg tttttcaact cccaggcctt
     1021 gtcagaggtg gtgaacagca cggtggcctc gaggttgttc tctttcatgc gcgttgtgtc
     1081 gagggagagg ggcgctatct tgtaggtgat gagctcgttg atgttcttca tgtacctggg
     1141 ctcgtggttt atctgggatg gcttgatgag gggcgcgtag tagatcgcct gctcgccgcc
     1201 cccgtactgc tcgggcatcc gctgcttgaa gctgttcagg tcgcatacca gggacatctc
     1261 caccttcacc ccgtagtggg ccaggagcct ttcgatgccg gtcgtcaccg gcatcagctg
     1321 gccgggctgc atcggctggt tgggatccgc aggtctttcc acgaacggat cgagaaggac
     1381 ggcgatgttg gtcccgcgca tgagcgcctg gtcgatctgg aagagctcgt agtcggtgaa
     1441 ggcctccgtg ggcctgacga tgagcaggca cccgagacct tcgggtatcc ccttgtcgag
     1501 ggtgatctcc ttgatggtat agacgcggga cgccaggtcc tggaagttgt tcagggaggc
     1561 gctcgagccc ggttggccgt acaggccgag cgtgcccttg tccgcgaggt agcccagggt
     1621 ctcgttgatc cccaccagcg actcgatact ctgctcgagc gcctcggcca tcttttcgac
     1681 gggcgtcagg cggtactgcg tgccgaacag ggggatggtg tacgcctgga tcaccgggat
     1741 cgtgtaagac ctgcccccgt attccaccac catgcccgcg aggcccgcac ccgccttcac
     1801 cctgccgccg tcgagatcgg gccacgtgag catcatcagg ttgtacgtgc gggaggcctc
     1861 ctccatcgcc tgcctcccgt cgaggtccac cctcctgtag gatatggcgt tgaagaggcg
     1921 ggcgttgagg tcgcccaccg ccttttcgac ctgggcgggc acccggtcga ggtccttgag
     1981 agacatgcgg ctcgagacgg cctcgaggga cgaggaaagg tagagcttca cctccacctt
     2041 gtccttgagc cccgacagca cgctgatctt gttggcgagt ttctgcatgg acgtggtgag
     2101 cttgtactcc agcccctccg tcgtggtgag ggccgggatg cgctcgatca tgtccccgtg
     2161 gacgatcgcc agccccatgt atgcacgctt gaacttgacc tcgtccttct ccacgacctg
     2221 gatctgtacc ggggttatgc cgtagtcgga tgcgagcctc ctggtgtcga ccccgacgtc
     2281 cggctgttga gcgtcctctt ctcccacgcg gtagaaccgg tagttgaaac tcttgttcgc
     2341 atagagggcg tattcggaga gcaggtctct caggtactgc tcgaccgtgt tgtagggcgc
     2401 gggcagatcc ggggtgaaga acacgttgat ggtcatgggt tccttgagtg agccgacgac
     2461 cgtcttgctc acctccgaca gcgagtacat cctgttcgcc gtgaggtcga acctcaaaaa
     2521 gagcgtcgat gccgccgcat tgatgaggat gacgagcgcc aggtagacga gaaacttgac
     2581 gatgcgccca tgtctctgtg ctctcatggc tcacctcttt tcctgcatga cgaggtgcgc
     2641 gccgtagagg gacaggaaaa cgacgcttgc gaagtagacg atgtcccggg tgtccaccac
     2701 acccttggcg atgttctcga agtgcgacga ggcgcccagg taatggagca cggcgagcac
     2761 gtccggcggg aagaagaaga gcatcttgtc aacgaggctc agggcaaggc agatgaccat
     2821 gcccaggatg aacgagatga tctggtttct cgtcaaggtc gatgcgaaga cgcctatggc
     2881 gcagtatgcc gctcccagga ggaccgcccc tgcgtacccg cccgccaccg ggccccagtc
     2941 gagaccgccc atgaacgaga cccagatccc gtacgacagg gtgggcgcga gcatcaccgc
     3001 cacgaaggcg ctgctcgcga ggaacttgcc gatgaccgcg tccgagaggt ccatggggag
     3061 ggtcaggagc atctcgaggc tgcccgagtg gagttcctcg gcgaacagtc gcatggtgac
     3121 ggccgggacc acgaagggga agacgagcgg cagcatggag aagaaggtcc tcagggacgt
     3181 ctggttcatg atgaagaagg tggcgaagaa gaaccagccc gtgacgacca agaacgcgca
     3241 gatcacgatg taggcgatcg gcgtggtgaa gtagtccatg aattccct
//
LOCUS       DAOSCR010000284         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18658_length_3285_cov_3.296594, whole genome shotgun sequence.
ACCESSION   DAOSCR010000284 DAOSCR010000000
VERSION     DAOSCR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18658_length_3285_cov_3.296594"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLT69_07600"
     CDS             <1..194
                     /locus_tag="PLT69_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14338.1"
                     /translation="GPFSMTNQKVRKIFYVIGVGKEADGEGLFTTSLGQRDITWNRRR
                     SSEGRRPASKVLKNLDTIV"
     gene            complement(365..1036)
                     /locus_tag="PLT69_07605"
     CDS             complement(365..1036)
                     /locus_tag="PLT69_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOJ14339.1"
                     /translation="MDDMALYLESTPTMDFDREPVREYAQRAAGAGGGLVDKAVRLYY
                     AVRDDIRYDPYTSVNLTVEGLRASTTLETRRGWCVAKAILLAACCRAVGIPARLGFAD
                     VRNHLTTSRMRELMKTDVFFWHGYASLYLEGKWVKATPAFNIELCTKFRLAPLEFDGR
                     SDSIYHPFDLQGNRHMEYLAYRGEFADVPIDAIRQTFLECYGGADLEGPVDFDAEVER
                     EVLEG"
     gene            complement(1155..1790)
                     /locus_tag="PLT69_07610"
     CDS             complement(1155..1790)
                     /locus_tag="PLT69_07610"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOJ14340.1"
                     /translation="MSAHDTKVYVTDVEERLVHWILAASCLFCVLTGVGLMFHSWSII
                     PTLLGGYYATKLMHIYSGVVFAGMLVYSYYLWRKDCGFEESDLDWVLKAGGYLWPVKE
                     LPPVYKYNAGQKAFFWCLIVTGVVIVVTGLFMWLWNPASVPNVLARIMYMLHAGAVVV
                     LAAFIMIHIYLGTAGNPGTVSAMITGYVTKAWCKTHCPRWYQEKFEKDAQA"
     gene            complement(1793..2575)
                     /locus_tag="PLT69_07615"
     CDS             complement(1793..2575)
                     /locus_tag="PLT69_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ14341.1"
                     /translation="MAEYIKLIDVTKCTGCRGCQVACKQWNSLPAEKTIFSGSLQNPK
                     DLTYNTWTLIRYDEVVKDGKLHWVMRHDACMHCDWPACAKACPSPGALVKTPEGAVVH
                     NPQYCIGCKACIAACPFDIPRNNPELEKMAKCTLCYDRITEGKQPACVTACPTGCLTF
                     GLKEDMLEKAHKRAEAVGGTIYPSNPKYETHVMYILPAGVSPKDLQHTNPNPSMPLSV
                     LLWKNLFKPFTLIGMGAVIGGAFLHYLIKGPHEVHETKEGGE"
     gene            complement(2587..>3285)
                     /locus_tag="PLT69_07620"
     CDS             complement(2587..>3285)
                     /locus_tag="PLT69_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOJ14342.1"
                     /translation="WDPLSKTWKGDIPDGPWPPQDKLPFIMVPHGRAMLFAKGMADGP
                     FPEHYEPLETQVQNFLSSQQVSPVIARWDKKAKEETCDPLIAEGCIAATFGSEEAKDF
                     PIICTTYRVVEHWQTGQMTRWLPWLAEMVPNQFVEISEELAREKGIVNGQKIRLTSIR
                     NVEGVEAYAMVTKRLKPFVIKGKTYHMVGTTWHFGYKGLVTGGNMNDLTPFIGDPNTM
                     IPEYKAFLVNIEKV"
BASE COUNT          563 a         1034 c         1053 g          635 t
ORIGIN      
        1 gaggaccttt ttcaatgaca aaccagaaag tgcgaaaaat attttacgtt atcggggtgg
       61 ggaaggaggc agacggcgaa gggctgttca ccacaagcct cgggcaaagg gatatcacgt
      121 ggaacaggag gcgctcctcc gaagggcgga gacccgcatc aaaagtgctt aagaatcttg
      181 acaccatcgt ttgacgagcg caggaggatc tcctccggag gagaaaggcc cgaatgaaca
      241 gaggggaaac agcggggaac actctcgagg ccaccccccg gggcaggcgc tctcgcgccg
      301 tgccgggccc ggccgggaag gccccggccc caggcgaggg atctgtgccg gggaaagacg
      361 ccgctcaccc ctcgaggact tcgcgctcca cctcggcgtc gaaatccacc gggccctcca
      421 ggtcggcacc gccgtagcac tcaaggaagg tttgcctgat ggcgtcgatg ggcacgtctg
      481 cgaactcgcc ccggtaggcg aggtattcca tgtggcggtt cccctggaga tcgaaggggt
      541 ggtagatcga gtccgacctg ccgtcgaact ccaggggtgc gagcctgaac ttcgtgcaga
      601 gctcgatgtt gaaggccggc gtggccttca cccacttccc ttccaggtag agggaggcgt
      661 agccgtgcca gaagaacacg tcggtcttca tgagttccct catgcgcgag gtggtgaggt
      721 ggttcctcac gtccgcgaag ccgagccgcg cggggatccc cacggcacgg cagcaggctg
      781 ccaggagtat ggccttggcc acgcaccacc cccttcgcgt ctcgagcgtg gtgctcgccc
      841 tgagcccttc gaccgtgagg ttcacggagg tgtacgggtc gtacctgatg tcgtccctca
      901 ccgcgtagta gagcctcacg gccttgtcca ccaggccccc gccggcgccg gctgcgcgtt
      961 gagcgtactc cctcaccggc tccctgtcga agtccatcgt gggtgtgctc tcgaggtaga
     1021 gcgccatgtc gtccatgaac cggtcctcct tgtcccctgc atcggcacgg cattacatcg
     1081 gaaaacacga gagggggccc tcacctcagg ccccctcgca tttcgcctcg tgcaggcggg
     1141 gtcgatgacc gcctctacgc ctgcgcgtcc ttctcgaact tctcctggta ccacctcggg
     1201 cagtgggtct tgcaccaggc cttggtgacg tagccggtga tcatggccga caccgtgccg
     1261 gggttgcccg cggtccccag gtagatgtgg atcatgatga aggccgcgag caccaccacg
     1321 gctcctgcgt gcagcatgta catgatcctc gcgagcacgt tcggcacgga ggccgggttc
     1381 cacagccaca tgaacagccc ggtgaccacg atcacgacgc ccgttacgat gaggcaccag
     1441 aagaatgcct tctggccggc gttgtacttg tagaccgggg gaagctcctt gacgggccag
     1501 aggtaaccgc cggccttgag cacccagtcc aggtcgctct cttcgaaccc gcagtccttg
     1561 cgccacaggt agtacgagta gacgagcatg cccgcaaaga ccacgccgga gtagatgtgc
     1621 atgagcttcg tcgcgtagta gcccccgagc agggtcggga tgatgctcca cgaatggaac
     1681 atcaggccca caccggtcag cacgcagaac aggcagctcg cggccaggat ccagtggacc
     1741 aggcgctctt cgacgtccgt aacgtagacc ttggtgtcat gtgcagacat ggtcattcac
     1801 ccccttcctt tgtctcgtga acctcgtgcg gccccttgat gaggtagtgg aggaaggcgc
     1861 cgccgatgac cgcgcccatg ccgatgagcg tgaagggctt gaacaggttc ttccacaaca
     1921 gcacggagag gggcatggac gggttcgggt tcgtgtgctg caggtccttc gggctgacgc
     1981 ccgcgggcag gatgtacatg acgtgggtct cgtacttcgg gttgctcggg tagatcgtgc
     2041 cgccgacggc ctcggcacgc ttgtgtgcct tttcgagcat gtcttccttg agcccgaagg
     2101 tgaggcagcc ggtggggcat gcggtcacgc aggccggctg cttgccctcg gtgatgcggt
     2161 cgtagcacag cgtgcacttc gccatcttct cgagctccgg gttgttgcgc gggatgtcga
     2221 aggggcatgc cgcgatgcac gccttgcacc ctatgcagta ctgggggttg tggaccactg
     2281 cgccctcggg cgtcttcacc aacgccccgg gggacgggca cgccttcgcg cacgcgggcc
     2341 agtcgcagtg catgcacgcg tcgtgccgca tcacccagtg gagcttgccg tccttgacca
     2401 cctcgtcgta gcggatcagg gtccaggtgt tgtatgtcag gtccttcggg ttctggagcg
     2461 agccggagaa gatggtcttc tccgcgggca gagagttcca ctgcttgcat gccacctggc
     2521 agccccggca cccggtgcac ttggtcacgt cgatgagctt gatgtattca gccatgtctc
     2581 accccctcac accttctcga tattgaccag gaaggccttg tactcgggta tcatggtgtt
     2641 cgggtcaccg atgaacggcg tgaggtcgtt catgtttccg ccggtgacaa ggcccttgta
     2701 cccgaagtgc caggtcgtac ccaccatgtg gtacgtcttg cccttgatga cgaacggctt
     2761 gagcctcttg gtcaccatgg cgtaggcctc gacgccctcg acattcctga tggaggtgag
     2821 gcgtatcttc tggccgttga cgatgccctt ctccctcgcg agctcctcgc tgatctccac
     2881 gaactggttg ggtaccatct cggccagcca cggcagccac ctggtcatct ggccggtctg
     2941 ccagtgttcc accacgcggt aggtggtgca gatgatgggg aagtccttgg cctcctcgct
     3001 gccgaaggtg gccgcgatgc agccttccgc gatgagcggg tcgcaggtct cctccttcgc
     3061 cttcttgtcc cacctcgcga tcacgggcga cacctgctgg ctggagagga agttctgcac
     3121 ctgtgtctcg aggggctcgt agtgctccgg gaacggcccg tccgccatgc ccttggcgaa
     3181 gagcatggcc ctgccgtgcg gcaccatgat gaagggcagc ttgtcctggg gcggccacgg
     3241 gccgtccggg atgtcgccct tccaggtctt ggagagcggg tccca
//
LOCUS       DAOSCR010000285         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18690_length_3281_cov_3.664910, whole genome shotgun sequence.
ACCESSION   DAOSCR010000285 DAOSCR010000000
VERSION     DAOSCR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18690_length_3281_cov_3.664910"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..801)
                     /locus_tag="PLT69_07625"
     CDS             complement(31..801)
                     /locus_tag="PLT69_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14343.1"
                     /translation="MGEPVRIAICGKGGVGKTSVAAVMVNCLKGEPGPRRILAIDADP
                     ACGLSLALGVEVKKTVDDIRREVVSDAKRGAARPEPLTVRRIEHDLFDALHEQDGFSL
                     LSIGRPEDEGCFCKVNSLLKDLIAELAHAFDFVVIDGEAGVEQINRRVMKQVDYLVAV
                     TDTSLKGIEVARTIAHLALDNAAVDCRELGLVVNRARSEEEASMVCARSGLEAWGWIP
                     EEEAVRRLDLEGVPLHALGQDSPVVRSVRSVLSRMITR"
     gene            complement(794..1528)
                     /locus_tag="PLT69_07630"
     CDS             complement(794..1528)
                     /locus_tag="PLT69_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14344.1"
                     /translation="MELFAETIQLLRSFAAAAGGRRLDAGARPPWPPGGRGCVVFKED
                     TVLELGGPSTESMSAVLWTCGAEEVHGGSITLVGPDFAEARTPGLPLGLVNIVKVDTV
                     EPDDAAYLMRDLELVPFELDLKGFMVRSSSVKHSLRCRIGTQAPRDCVCAAGLAGALG
                     RLYLEKPRVLAVETLIVTSAGRDMEDLRKILDPALAKIEALYRAASGEEFDCASCDYR
                     DVCSEVEEIRRLHEAFMRKTREAVNG"
     gene            complement(1597..2391)
                     /locus_tag="PLT69_07635"
     CDS             complement(1597..2391)
                     /locus_tag="PLT69_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ14345.1"
                     /translation="MITAGLDIGSLSAKALILRDDEILGWHVIAARPDPEDSAREVMA
                     GALGKAGLSMDDIEFTVGTGYGRKAVPFARAVESEISCHGMGAGWLMPGVRTVIDIGG
                     QDAKAVRIDDSGRVVRYAYNDKCASGTGRFLEIVAGVMGIGLDELGPVSLRARNPVSL
                     SNQCVVFAETEIISLINEGRDKADIVAGLHRALAHRVASLARSIEPEPAVAMTGGVAK
                     NPGMFKALEEALGTGITRCDIDPQAIGALGAALIARERAAKGGAGA"
     gene            complement(2388..3176)
                     /locus_tag="PLT69_07640"
     CDS             complement(2388..3176)
                     /locus_tag="PLT69_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ14346.1"
                     /translation="MIVSGCDVGSLATKAVILNERSVLAQAVVPSSFDPARSADEAMG
                     RALEQAGLRLSDIRYCVGTGYGRERIPFVSKAVSEIACHAKGAHWLIPTARTVIDIGG
                     QDCKAIRLDARGNVVKFLTNDKCAAGTGRFLEVMARILGLGLEELGNQRGKAGEPIQL
                     ASTCTVWAQAEVIHHLNAKKDIADIAHAVNRAMAARVAILARGVGIERDLVMTGGVAK
                     NKGVTSTLEELLGTGIRRLRLDPQVIGALGAAVIAKEQAEGGKA"
BASE COUNT          498 a         1185 c         1025 g          573 t
ORIGIN      
        1 ggagctctgc atccccggcc gaggcgtcgt ttaccgtgtg atcatgcggg acaggacgga
       61 gcgcacggat ctcaccaccg gcgagtcctg cccgagggcg tgcagcggga cgccctcgag
      121 gtccagcctg cgcaccgcct cttcctcggg tatccagccc cacgcctcga ggccggatct
      181 ggcgcacacc atgctggctt cctcttcgct gcgggcccgg ttcacgacga gacccagctc
      241 cctgcagtcg accgcggcgt tgtcgagcgc gaggtgcgct atggtgcggg cgacctcgat
      301 ccccttcagc gaggtgtccg tgaccgcaac caggtaatcc acctgcttca tgaccctcct
      361 gttgatctgc tcaacacctg cctccccgtc tatgaccacg aagtcgaagg cgtgcgcgag
      421 ctcggcgatg aggtccttga ggagggagtt caccttgcag aagcatccct cgtcctcggg
      481 cctccctatc gagaggaggg agaagccgtc ctgttcgtgc agggcgtcga agaggtcgtg
      541 ctcgatccgc ctcacggtca gcggctcggg cctcgcggca ccgcgcttcg catcggagac
      601 gacctccctg cggatgtcgt ccaccgtctt tttgacctcc accccgaggg cgagcgagag
      661 accgcaggca gggtccgcgt ctatggcgag gatcctcctc ggccccggtt cgcccttgag
      721 acagttcacc atgaccgccg ccaccgaggt cttgccgacc ccgcccttgc cgcagatcgc
      781 tatacgtacg ggctcaccca ttgacagcct ccctcgtttt cctcatgaac gcctcgtgaa
      841 gtctcctgat ttcctccacc tccgagcaca cgtcgcggta atcgcacgat gcgcagtcga
      901 actcttcgcc ggaggccgcc cggtacaacg cctcgatctt cgccagtgcg gggtccagga
      961 tcttccgcag atcctccatg tcccggccgg cagacgtgac gatgagcgtc tccacggcca
     1021 ggacgcgggg tttctccagg tacaggcggc ccagggcgcc agccaggccc gccgcgcaga
     1081 cgcaatcccg gggcgcctgg gtcccgatcc tgcacctgag actatgcttg accgaggagg
     1141 aacggaccat gaaccccttg aggtcgagct cgaagggcac gagctcgagg tcccgcatga
     1201 ggtacgccgc gtcgtcgggc tccacggtgt cgaccttcac gatgttcacg aggcccaagg
     1261 ggagcccggg cgtacgggcc tctgcgaagt cgggacccac gagggtgatc gagcctccgt
     1321 ggacctcctc ggccccgcag gtccacaaga ctgcgctcat ggactcggtg gagggcccgc
     1381 cgagctcgag caccgtatcc tccttgaaga ccacgcaccc cctgcccccc gggggccacg
     1441 ggggccgtgc gccggcgtcc agcctcctgc cgccagcagc cgcggcgaac gacctgagga
     1501 gctgtatggt ctctgcgaag agttccatgc caccttccat ggccctcggg gaccagcccg
     1561 agaagcgcct gtcttgccgg tgacgccccg cctggttcac gcgccggccc cacccttggc
     1621 cgccctctcg cgcgcgatga gggcggcacc gagggcgccg atggcctgcg ggtcgatgtc
     1681 gcacctggta atgcccgtgc cgagcgcctc ctcgagcgcc ttgaacatgc cggggttctt
     1741 cgcgacgccc ccggtcatcg caacggcggg ttcgggctct atggagcggg cgagagacgc
     1801 caccctgtgg gcgagcgccc ggtgcaggcc ggcgacgatg tcggccttgt ccctgccctc
     1861 gttgatgagg gatatgatct cggtctcggc gaagaccacg cactggttcg acagcgagac
     1921 cgggtttctg gcccgcaagg agacagggcc cagctcgtcg aggcctatgc ccatcacgcc
     1981 cgccacgatc tcgaggaacc tgccggttcc cgaggcgcac ttgtcgttgt acgcgtacct
     2041 cacgacccgg ccgctgtcgt ctatcctcac ggccttggcg tcctggccgc cgatgtcgat
     2101 gacggtgcgc acgcccggca tgagccatcc cgcgcccatg ccgtggcagg atatctcgga
     2161 ctcgacggcc ctggcgaacg gcaccgcctt cctcccgtac cccgtgccga ccgtgaactc
     2221 gatgtcgtcc atcgagagac cagccttccc gagtgcaccg gccatgacct ccctagcgga
     2281 atcctcgggg tccggcctcg cggcgatcac gtgccagccg aggatctcgt cgtccctcag
     2341 gatgagggcc ttggcgctca gcgacccgat gtccagtccc gcggtgatca tgccttgccc
     2401 ccttctgcct gttccttcgc gatgaccgcg gcacccaggg cgccgatgac ctgggggtcc
     2461 agcctgagcc tcctgatgcc tgtcccgagg agctcctcga gcgtcgaggt cacccccttg
     2521 ttcttcgcga ccccgcccgt catcaccaga tccctctcga tgccgacgcc ccgcgccagg
     2581 atggcgacgc gggcggccat ggccctgttc acggcgtgcg cgatatcggc tatgtccttc
     2641 ttggcgttga ggtgatggat gacctcggcc tgggcccaca cggtgcaggt gctggcgagc
     2701 tggatgggct cgccggcctt gccccgctgg ttccccagtt cctcgaggcc caggcccagg
     2761 atcctcgcca tgacctcgag gaagcggccc gttcctgcgg cgcacttgtc gttcgtgagg
     2821 aacttcacca cgttgccccg tgcatccagc ctgatcgcct tgcagtcctg gccgccgatg
     2881 tcgatcaccg tcctcgccgt gggtatgagc cagtgggccc ccttggcgtg gcaggcgatc
     2941 tccgagaccg ccttcgacac gaagggtatg cgctccctcc cgtagccggt gcccacgcag
     3001 taacggatgt ccgacagcct gagcccggcc tgctcgaggg ccctgcccat ggcctcgtcg
     3061 gccgagcgcg ccgggtcgaa gctcgatggg acaacggcct gggccagtac cgaccgttcg
     3121 ttgaggatca ccgccttggt ggcgagagac cctacgtcgc aacctgacac gatcatgaga
     3181 tgatccccct gacagtgaga aattcggaca acctttccgt gaccgtctcc cacgacgcaa
     3241 ccctgtcgtc gaaggcgtcg gcgaagagta tgacggcagg g
//
LOCUS       DAOSCR010000286         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18722_length_3277_cov_3.202980, whole genome shotgun sequence.
ACCESSION   DAOSCR010000286 DAOSCR010000000
VERSION     DAOSCR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18722_length_3277_cov_3.202980"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLT69_07645"
     CDS             complement(<1..397)
                     /locus_tag="PLT69_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOJ14347.1"
                     /translation="MGCAEDPPPFDRLLVAACYEGDLRDMIHAFKYRRCTHLKGYLGS
                     LLYEAVKDELKECDLVTAVPLHWSRVLARGYNQSMLLAREVSRRSGVRLAPGVLRRTR
                     PTKAQVGLNPSERKKNLKGVFSARDVEGLS"
     gene            complement(943..1125)
                     /locus_tag="PLT69_07650"
     CDS             complement(943..1125)
                     /locus_tag="PLT69_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14348.1"
                     /translation="MDAIIMVARYRYTREAEFKMSFEALPKEKVLGTVLNAVDELPVK
                     KYTYRKYKYYKDYYNR"
     gene            complement(1159..1710)
                     /locus_tag="PLT69_07655"
     CDS             complement(1159..1710)
                     /locus_tag="PLT69_07655"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ14349.1"
                     /translation="MQILKAFEKQEREQATQAGALGPLDDHLYMYHMPYSYTAEQIRK
                     LRTHIFHLPDGRPAPRVIMVTSALPGEGKTLIASNLAVAIAKGEDQRVLLVDCDLRKP
                     SLNDVFKFSRSKGVSEILQGRAEIAECIISTPIDKLAILPAARENPANPSELLESKRA
                     AALVKDLAALGEDWFIIIDTPPL"
     gene            complement(1715..>3277)
                     /locus_tag="PLT69_07660"
     CDS             complement(1715..>3277)
                     /locus_tag="PLT69_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14350.1"
                     /translation="DPKFLYMSPQHKEALAHLLYGLKEGSGFVVITGEVGTGKTTILN
                     AFLLKLPPRIPKVVIKNPHIKPENLYYLLGEAIGMPEDKRSRDFLGEFEERLKATGGA
                     VLICDEAQGLSFEMLEEIRLLSNLETVHEKLVQIMLLGQQELNDKLNSHQLRQLKQRI
                     GVKYNIPPLDLGETRDYIDHRLRVAGYEPKEDPLFTPSAIGEIYRYTRGFPRLINIVC
                     DNVLLAAYSDDLHQVSASLVKKVVSGIEKTYARHASKVPAHHIRRETAVPRWATVTMS
                     ILLAVTLASGIALFLVSPQGRALLDGAVSSILSDDKVKTGAHGSMGGAGRRVTVATPG
                     PGAGIQAPGNETDGSMPAGLEASDAPGYGERTGPGPTPAPQAWRTGGARSVVVGPGDT
                     LAKIAARHYGRVDAGILRLLAQANRDIRDINLVYEGQRIILPPVSSQARELYTVSVAA
                     YRLIDEATPVFLDLVKRGYPATIYAVKDADGKVWYRVSIGTFTSVEEANGYAGRLRAE
                     GFRFAKVMGVQG"
BASE COUNT          521 a         1102 c         1009 g          645 t
ORIGIN      
        1 ccgacagccc ttccacgtca cgggcggaga acaccccctt gaggttcttc tttctctccg
       61 aagggttgag acccacctgg gctttcgtgg gccgggtcct cctcagcacg ccgggtgcca
      121 gcctcacccc tgatctcctg gacacctcgc gtgccaggag catcgactgg ttgtaccccc
      181 tcgcaaggac cctgctccag tgcagcggta cggcggtcac caggtcgcac tccttcagct
      241 cgtctttgac ggcctcgtag aggagggacc ccaggtaacc cttcaggtgg gtgcaccgcc
      301 tgtacttgaa tgcatggatc atgtccctga gatccccctc gtagcacgcc gccacgagaa
      361 gcctgtcgaa cgggggggga tcctccgcgc accccatgca tacaccatca atgccgaggg
      421 gcctcccgca tacccggcac gcgggtacgg gcaagggtct caccgaagag gtgcagcacg
      481 cacccacccc gcacgtctga ccgtccaccc ttcttccgca cacgacgcac ctgggtggga
      541 agacgtacgg gagtatccgg gagagcattg ctcgaacata ccacaggcag gcacgcacga
      601 caacaaacag gccgaacccg aaggcgccag gtcgccttct tcccgcctca tgaatgacgg
      661 ggacagggat cgaacgccag gcacaagaga ccgtcccgtc ctcgccgcct caccgggtag
      721 cgatcgtctc cggatcttcc ggctttttcc cccatcgccc gagcttctcc acgacggtgt
      781 tcggcttccc gccgggcctt ccgaaaccgc ccggagacgc cttgcgtgtg ggtggccgtg
      841 catccggccg cttgcatgcg gccctcagga gacggcccat gcccgccggg aggtcaggga
      901 aggggtcgtg acgcgccgat cgcgagtgac ggcaggtctt tctcagcggt tgtagtagtc
      961 cttgtagtac ttgtacttgc ggtacgtgta cttcttcacc ggcaactcgt ccacggcgtt
     1021 gagcaccgtt ccgagaacct tctccttggg caacgcctcg aaggacatct tgaactcggc
     1081 ctccctcgtg taccggtacc tcgccaccat gatgatggcg tccacgaccg tggagatgat
     1141 cctggggtcg accgtggcct acaagggcgg ggtgtcgatg atgatgaacc aatcctcacc
     1201 gagggccgcc aggtccttca cgagggccgc ggccctcttg gactcgagga gttccgaggg
     1261 gttcgccggg ttctcacggg cggctgggag gatggcgagc ttgtcgatgg gggtggagat
     1321 gatgcactcc gcgatctccg ccctgccctg gaggatctcg ctgacgccct tgctccggct
     1381 gaacttgaat acatcgttga gcgacggttt cctcaagtcg cagtccacga gcaggaccct
     1441 ctggtcttcg cccttcgcta tggccaccgc gagattcgat gcgatcagcg tcttcccttc
     1501 gccgggcagc gcgctcgtca ccatgatcac cctcggcgcc ggccttccgt ccggcaggtg
     1561 gaagatgtgc gtgcggagct tgcgtatctg ctcggccgtg tacgagtacg gcatgtggta
     1621 catgtacagg tggtcgtcca ggggcccgag ggcccccgcc tgggtcgcct gttcccgctc
     1681 ttgtttctcg aaagccttca gaatctgcat gacctcatcc ttgaacgccc atgaccttcg
     1741 cgaaccggaa accctcggcc ctgagcctgc ctgcgtaccc gttcgcctcc tcgaccgagg
     1801 tgaacgtgcc tatggagacc ctgtaccaca ccttgccgtc ggcgtctttc acggcgtaga
     1861 tcgttgccgg gtagcccctc ttcacgaggt cgaggaacac gggggtggcc tcgtcgatca
     1921 ggcggtatgc ggccacgctc acggtgtaga gctctcgggc ctgcgaggag accggcggca
     1981 ggatgattct ctgtccctcg tagacgaggt tgatatcgcg gatgtccctg ttcgcctggg
     2041 ccaggagacg caggatcccc gcatccaccc tcccgtagtg acgcgctgcg atcttggcga
     2101 gcgtgtcccc cgggccgacg acgacgctcc tggccccacc cgtgcgccac gcttggggcg
     2161 caggtgtcgg acccggccca gttctctccc catatcccgg ggcatccgac gcctccagcc
     2221 ccgcgggcat cgacccatcc gtctcgtttc cgggggcttg tatcccggca cccggccctg
     2281 gggtggctac cgtgacccgc cggccagccc cgcccatgct tccgtgtgcc ccggtcttca
     2341 ccttgtcatc gctcagtatc gacgacacgg ccccatccag cagggccctc ccctgcggtg
     2401 acaccaggaa gagggcgatg ccgctggcga gcgtgacagc gagcaggatg gacatggtga
     2461 ccgtcgccca cctgggaacc gcggtctccc tcctgatgtg gtgggcaggc accttcgatg
     2521 cgtgccgtgc gtacgtcttc tctatgcccg agaccacctt cttcaccagc gatgcgctca
     2581 cctggtgcag gtcgtcgctg taggccgcga gcaacacgtt gtcgcacacg atgttgatga
     2641 ggcgggggaa ccccctggtg taccggtaga tctcgccgat cgccgagggg gtgaagaggg
     2701 ggtcttcctt cggctcgtaa cccgccaccc tgagcctgtg gtcgatgtag tcgcgggtct
     2761 cgccaagatc cagggggggg atgttatact tgacgccgat gcgctgcttg agctgcctga
     2821 gctggtggct gttcagcttg tcgttgagct cctgctgtcc caggagcatg atctgcacga
     2881 gtttctcgtg cacggtctcg aggttcgaca ggagcctgat ctcctcgagc atctcgaagg
     2941 aaagcccctg ggcctcgtcg cagatgagca ccgccccgcc ggtggccttg agcctctcct
     3001 cgaactcgcc caggaagtcc ctcgacctct tgtcctcggg catgccgatc gcctccccga
     3061 ggaggtagta caggttctcg ggcttgatgt gggggttctt gatgaccacc ttgggtatgc
     3121 ggggcggcag cttgaggagg aaggcgttga ggatggtcgt ctttccagtc cccacctcgc
     3181 cggtgatgac cacgaacccg ctcccctcct tcaaaccgta gagcaggtgc gcgagggcct
     3241 ccttgtgctg cgggctcatg tagagaaact tcgggtc
//
LOCUS       DAOSCR010000287         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18767_length_3270_cov_2.957387, whole genome shotgun sequence.
ACCESSION   DAOSCR010000287 DAOSCR010000000
VERSION     DAOSCR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18767_length_3270_cov_2.957387"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLT69_07665"
     CDS             <1..845
                     /locus_tag="PLT69_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ14351.1"
                     /translation="LTNPGDLEDYLEVVGKARPLANPCAPLIAVPTTAGTGSEATKNA
                     VLISKEHGVKVSLRSIMLLPALVLVDPALTLSLPPRLTACTGLDALTQLLEAFVSRAS
                     NPMTDCLCREGLSRAAGSLLAAYRTGDAIEPRADMSLASLLSGMALANAGLGAVHGLA
                     AAIGGLYRAPHGFVCASLLAGVVEANILSLCRPGEPSRAIDKYTEASRILTGDSQEDP
                     ADLVRWIKRLCRELEAPSLSELGVDKAGFEPIVARALRSSSMRGNPVVLSEDELMDIL
                     AGAL"
     gene            complement(1104..1289)
                     /locus_tag="PLT69_07670"
     CDS             complement(1104..1289)
                     /locus_tag="PLT69_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOJ14352.1"
                     /translation="MSEKRFTTLETMVAYHEDTLQKLNDVVYKQQVMIDKLQDKLEAL
                     TRLVLGEERPVVDEAEK"
     gene            1840..2706
                     /locus_tag="PLT69_07675"
     CDS             1840..2706
                     /locus_tag="PLT69_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ14353.1"
                     /translation="MAWFALSLIAAFTQATSDALVKRWFSDLDPFTMGTARLFYALPW
                     LLPALFFIDVPPLDSAFYVSIACAIPVEILALVCYMEAVRSSPLSLVMPFAAFTPAFL
                     TITGSVILSETPSPMVLLGIGLIVCGAYCLGLPDARKGVWEPFKAIASQKGPRLMLLV
                     AFLYSVTSALGKRAILHSSPAFFGVTYFACLAIVMLGAALFITRSGLKRALLRPGPKA
                     AVGMVYATMIFSHTAAIAMTQAAVMIALKRTSMIFGIVYGAVLFRETGAWKRLPGAIL
                     MLAGVVILGLWA"
BASE COUNT          529 a         1135 c          986 g          620 t
ORIGIN      
        1 tgctgacaaa ccccggcgat ctcgaggact acctggaggt cgtggggaag gcccggccct
       61 tggcgaaccc ctgcgcgccc ctcatcgccg tgccgaccac cgccgggacc ggcagcgagg
      121 ccacgaagaa cgcggtgctc atctccaagg aacacggcgt gaaggtgagc ctgaggtcga
      181 tcatgctcct tcccgccctg gttctcgtgg acccagccct taccctgtct ctgccgccgc
      241 gtctgaccgc gtgcaccggc ctggacgcac tgacccaact gctcgaggca tttgtctcga
      301 gggcatcgaa ccccatgacg gactgcctgt gcagggaagg tctctcgagg gcagccggat
      361 cgctccttgc ggcctatcgt accggggacg ccatcgagcc tcgtgccgac atgtcgctcg
      421 cgagcctctt gagcggcatg gcgctggcga atgcggggct gggtgcggtg cacggcctgg
      481 ccgccgccat cggcggactg taccgggcac cccacggttt cgtctgcgcg tcgctccttg
      541 ctggcgtggt ggaagcgaac atcctttccc tctgcaggcc gggcgagcct tctcgggcga
      601 tcgacaagta cacggaggca tcccggatac tcacgggaga cagccaggag gaccccgcgg
      661 acctcgtccg atggatcaag cggctttgcc gcgagctcga ggcgccgagc ctgtccgagc
      721 ttggcgtaga caaggcaggc tttgaaccca tcgtggcaag ggcgctgcgc tcgagcagca
      781 tgaggggaaa cccggtcgtc ctgtccgagg acgagctcat ggacatactt gcaggcgcac
      841 tgtgagggtt cgggctcggc aggctcgcct gccgcatggg cccatggaca tctccccagg
      901 cgcaccgtca ggcgcgccct ctgtacgccg gtcaccgctc tttcagccca caggcccctc
      961 tacccgcatg aaaacaaggc cgtagacgct tcaggcgcac acacgagatt gcaccaggcc
     1021 ctgcaacaag aagactcgca cgaatcaggc cttccacgcc ctgtggcaag tccttcagaa
     1081 caagggcgaa gacgcgtcgg atctcacttc tccgcctcgt ccacaacagg cctttcctcc
     1141 ccgaggacga gcctggtgag cgcttcaagc ttgtcctgga gcttgtcgat catgacctgc
     1201 tgcttgtaga cgacgtcatt gagcttctga agcgtgtcct catgataggc caccatggtt
     1261 tccagcgttg tgaatctctt ctcactcata tgccccccac gtgtgtcctc tcattcgacg
     1321 accccgtgct tccaagacct ttatattata tgacccggcg ctcgagaatc aacccgcagg
     1381 tcacctcgtg tcaagactcg tgaggaatgt ccccttgttc acctcgttca caatgtaccc
     1441 tgccgcatgt cgatgtgagc accatgtttc aatcatggga gtgagcaagc cctatccgta
     1501 ggccgccgcc gggtccttcc cgggtcctcc cggggtcacc gggcggcata ggcagcgcag
     1561 cccatggcct ggggaggcgt catgggggta aggccgtctc gatcgcgagg ctcccacgcg
     1621 acccgttgac gtcgccagcg cgagacgccc gaagaagcga accgtcagcc ctgcctggac
     1681 ccgtcttgtc cgggcggcag gctcgccttt cccgtcgagc gcggaaggcg gcatgtcacg
     1741 ccggcgcccc tgtgtaccct ccagggaaac cgcgcctttc gctcgaccag gagatttttg
     1801 ctgttgatgt tgcagccgcc ggtgtgctcc atgtggtgta tggcgtggtt cgccctctcg
     1861 ctgatcgccg ccttcaccca ggccacctcg gacgcgctcg tcaagaggtg gttttcagac
     1921 ctcgacccgt tcacgatggg cacggcaagg ctcttctatg ccctgccgtg gctgttgccc
     1981 gctctcttct tcatcgacgt ccctcccctg gacagcgcct tctacgtgtc catcgcctgt
     2041 gcgatacccg tcgagatcct ggcgctcgtg tgctacatgg aggcggtcag gagctcgccc
     2101 ttgtccctcg tgatgccctt tgcagccttc acacccgcat tcctcaccat cacgggcagt
     2161 gtcatcctct cggagacgcc ctcccccatg gtgttgctcg gcatcgggct catagtctgc
     2221 ggcgcctact gcctcggcct tcctgacgcc cgaaagggtg tgtgggaacc cttcaaggcg
     2281 atcgcctcgc agaagggccc caggctcatg ctcctcgtgg ccttcctgta ctcggtgacc
     2341 tcggccctcg ggaagcgggc catcctccac tcttctccgg ccttcttcgg cgtcacctat
     2401 ttcgcctgcc tggcgatcgt catgctcggg gccgccttgt tcatcacgcg ttcaggtctc
     2461 aagcgggccc tgctgaggcc gggcccgaaa gcggctgtgg gcatggtcta tgcgacgatg
     2521 atcttcagcc acacggcggc catcgccatg acccaggccg cggtcatgat agcgctcaag
     2581 aggacgagca tgatcttcgg catcgtctac ggcgccgtgc tcttccggga gacaggtgca
     2641 tggaaacgac tgcccggagc gatcctcatg ctcgcaggtg tcgtcatcct cggactgtgg
     2701 gcatgaccgg ctgaacctcg gtgagcccac agcgtttcgt tacggggtgg tgcggcgagc
     2761 ctgcagggtc ggcgcctgtc cagcccgcat cgtcctagga aggaatgtcc tcgtcaggga
     2821 accgtcgtga acatgcagca ccgttcgggc tgcaccccat aggcttgcct ggcctggttc
     2881 ttccccttcg ggcccgcacg cccgtgcagg gtggaggctc acctgggagc gtcgcccctc
     2941 gagggccgcc aacccgctgc atcgagccac cgaagacccg ccgggatccc cacgaagcca
     3001 cgcgccgcat ccagcgacaa ggatccagcg gcccttcttc gcggcggcat cccttgacga
     3061 cgccagtcgc ccgtccgagg aacgtcgtcc gccccaccag agccttctcg gcgcctgcgc
     3121 accccgagcg tggaggaatg gagaacgggc acgaaccatt acccgaagac ttgcgaggcc
     3181 ttctcaggtg gcaaacgccc cttcccaggt tcgggccggc cccatgccgg cggggcgtga
     3241 aggtgcaaag acccgacggc tgccgcggct
//
LOCUS       DAOSCR010000288         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18803_length_3265_cov_3.755140, whole genome shotgun sequence.
ACCESSION   DAOSCR010000288 DAOSCR010000000
VERSION     DAOSCR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18803_length_3265_cov_3.755140"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1124)
                     /locus_tag="PLT69_07680"
     CDS             complement(117..1124)
                     /locus_tag="PLT69_07680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOJ14354.1"
                     /translation="MRTERVCVIHLNQIGDLIFSLPLLKALRDHYEGACIHSVARPYL
                     AGLLRDSPYVDRVLARGQTLWSRLSLVLALRRERYDLLLTLARSEEALMLACLSGAKV
                     KAGFAHSPWDACLDVKETVEGHNSWRNNARLLDRLGIPVFKNDYVGLLRVDGVPAVEG
                     LPWRYAVFSPGASRRRLAKAWDEGKFARLIAALSMEFGLSCVLVGGSDTAACTAQIAS
                     EASRLLSGGVSQIVDTSGRLDLRSLVAVLRKAKLFVGIDSGVMHMASALDIPTVAIFG
                     PTDPAFVGPQNAHSAVVRKQLHCSPCYLRTDCAHRRCLEEIEVEDVMRACRQVLSGLA
                     V"
     gene            complement(1140..2357)
                     /locus_tag="PLT69_07685"
     CDS             complement(1140..2357)
                     /locus_tag="PLT69_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ14355.1"
                     /translation="MEQLEKREHDFSAKLRQESVISALKDYVSWQRELRAGREDTVFP
                     VHGPVSINLDLTTACNFRCPHCVDSRLLNTGDYLDLETVRKSIDTLEKNGLKSVIIIG
                     GGEPTIHKNFQEIVRFIKHRGLQVGIATNGSRLEKVAEVARELSGKDWVRLSLDAARE
                     STFFKAHRPRGRTPLSGILEGASRIKDLNPEVQLGYSFVVVWEGIEVNGYELAANVDE
                     MELAVKNAQEHGFDYVSFKPCLVRLEGTMRESLFDPPDPQREERVIEQIRRNLASAQA
                     AASGRVRILKSVNLEAMLDKRVHDLKVQPETCHSQYFRTVLAPSGIFHCPALRGVEHA
                     RLADASGYRGRANFEFTHACLEHSIRSYNARVGCNTVVCFYHQVNWWIERFIASGEPV
                     ESIPVVGDDNFFL"
     gene            complement(2370..>3265)
                     /locus_tag="PLT69_07690"
     CDS             complement(2370..>3265)
                     /locus_tag="PLT69_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ14356.1"
                     /translation="ALRRNNGIGGFIARYLHAHGAKVVAVLGRTEAGAVEASHSLVRY
                     GIDARPFSDFDEMVAATSPQAVVIASPAPTHRRYLERCVDAGVHILCEKPFVDPKEPD
                     AVRFAGNILEEASKKGLTVAMNSQWPFCLAAYEELCGKIRPEGVESFSMRLSPQVGGI
                     DMIPDSMPHALSVLYCGAGPGGLSGVSMEKGATRLVVSFTYSTRSATFPVNVEMVREE
                     SQPRTFAFGFNGIVAQRLIRLPHYTISLTHGEKTLKIPDPLERSVQDFLQAMGTGGKP
                     LIGEEHIMQTLCGLVEVYSGY"
BASE COUNT          555 a         1076 c         1026 g          608 t
ORIGIN      
        1 gaggaccttt ttcaatgaca aaccagaaag tgcgaaaaat attttacgtt atcggtcgag
       61 gacgtcgtgc acgcccttta aggaacccgg cgacgcgcgt ggcgcgcccg atcgcctcag
      121 acagccagcc cgctcaggac ctgcctgcag gctcgcatga cgtcctcgac ctctatctcc
      181 tcgaggcacc ttctgtgcgc gcagtccgtc ctgaggtagc agggcgagca gtggagctgc
      241 ttcctcacca ccgcgctgtg ggcgttctgg gggccgacga acgcggggtc cgtgggcccg
      301 aagatggcca cggtcggtat gtccagggcc gaggccatgt gcatcacccc cgagtcgatc
      361 cccacgaaga gcttcgcctt cctgaggacc gcgacgagag acctgaggtc gagcctgccc
      421 gaggtgtcca cgatctggct cacgcctccc gagaggagcc tggacgcctc ggacgctatc
      481 tgcgcggtgc aggccgccgt gtctgatccc ccgacgagca cgcagctcag cccgaactcc
      541 atggacaacg ccgcgatgag acgcgcgaac ttgccctcgt cccacgcctt ggcgagcctc
      601 cggcgggacg cgccggggga aaagaccgcg tacctccacg gcagcccctc gacggccggg
      661 acgccgtcga cgcggaggag gccgacgtag tcgttcttga acaccgggat gccgagccgg
      721 tccaggagcc tggcattgtt cctccaggag ttgtgtccct cgaccgtctc cttgacgtcg
      781 aggcacgcat cccagggact gtgggcgaac ccggccttga ccttcgcacc gctgaggcag
      841 gcgagcatga gcgcctcctc ggatctcgcg agcgtcagga ggaggtcata gcgttcgcgc
      901 ctgagcgcaa gcacgagcga gagcctcgac cacagggtct gcccgcgggc caggaccctg
      961 tccacgtatg ggctgtccct gaggagcccg gccaggtagg gtctcgcgac cgagtggatg
     1021 caagcgccct cgtagtggtc cctgagcgcc ttcaagagcg gaagggagaa gatgaggtcc
     1081 cctatctggt tgaggtggat gacgcagaca cgctcggtac gcatccagga agcgcctcat
     1141 cagaggaaga agttgtcgtc cccgacgacc gggatgctct ccacgggctc tcccgaggcg
     1201 atgaaccgtt cgatccacca gttcacctgg tggtagaaac agaccaccgt gttgcagccg
     1261 acccgggcgt tgtacgacct gatggagtgt tccaggcagg catgcgtgaa ctcgaagttc
     1321 gccctccccc tgtaccccga cgcgtccgca agcctggcgt gctcgacccc cctgagcgcc
     1381 gggcagtgga atatgcccga gggtgcgagc acggtcctga agtactggct gtgacaggtc
     1441 tcgggctgca ccttcaggtc gtgcaccctc ttgtccagca tggcctcgag gttgacgctc
     1501 ttcaggatcc tcacccttcc cgaggctgcg gcctgggccg aagccaggtt ccgccggatc
     1561 tgctcgatga ccctctcctc tcgctgtgga tcgggcgggt cgaagagcga ctcccgcatg
     1621 gtcccttcca gcctgaccag gcacggcttg aacgacacgt agtcgaaacc gtgctcctgc
     1681 gcgttcttga cggccagctc catctcgtcg acgttcgcgg cgagctcgta gccgttcacc
     1741 tcgatcccct cccacacgac gacgaaggaa tagccgagct gcacctcggg gttgaggtcc
     1801 ttgatacggc tggccccctc gaggatcccg ctcaagggtg tccttccgcg ggggcggtgg
     1861 gccttgaaga aggtgctctc cctggccgcg tcgagggaca acctcaccca gtctttcccg
     1921 gagagctccc gggcgacctc ggcgaccttc tccagcctcg acccgttcgt ggcgatgccc
     1981 acctggaggc ccctgtgctt gatgaacctg acgatctcct ggaagttctt gtggatggtg
     2041 ggttcaccgc cgccgatgat gatgaccgac ttgagcccgt tcttctcgag cgtgtcgatg
     2101 ctcttcctca ccgtctcgag gtcgaggtag tccccggtgt tgagcagtcg cgagtccacg
     2161 cagtgcgggc accggaagtt gcacgcggtc gtcaggtcga ggttgatgga gacgggaccg
     2221 tgcaccggga acacggtgtc ctccctaccc gctcggagct cgcgctgcca ggacacgtag
     2281 tccttaaggg cgctgatcac cgattcctgc ctgagcttcg cgctgaagtc gtgttccctt
     2341 ttttcgagct gttccatgcg catccctcgt cagtaacctg aatagacctc aaccagacca
     2401 cacagggtct gcatgatgtg ctcctccccg atgagcggtt tcccgcccgt gcccatggcc
     2461 tggaggaagt cctgcacaga gcgttccagt gggtcaggta tcttgagggt tttttcgccg
     2521 tgcgtcaagg agattgtgta atgcggcaga cggatgagcc tttgtgccac gatcccgttg
     2581 aacccgaagg cgaacgtcct tggctgcgac tcctcccgca ccatctcgac gttcacgggg
     2641 aacgtcgcgc ttctcgtgct gtaggtgaac gagacgacga ggcgcgttgc gcctttctcc
     2701 atggagacgc cgctcagccc gccggggcct gccccgcagt acaggacgct cagcgcgtgc
     2761 ggcatggaat cggggatcat gtcgatgccc cccacctggg gggaaagcct catggagaac
     2821 gactcgacgc cctcagggcg gatcttcccg cagagctcct cgtaggctgc gagacagaag
     2881 ggccactggg agttcatggc gacggtgagg cccttcttcg aggcctcttc gaggatgttc
     2941 ccggcaaagc gtacggcgtc cggctccttg gggtccacga agggtttttc gcacaggatg
     3001 tgcacgcccg catccacgca gcgttcgagg tacctcctgt gcgtgggcgc gggggacgct
     3061 atgacgaccg cttggggaga ggtcgctgcg accatctcgt cgaaatccga aaacggcctc
     3121 gcatcgatgc cgtatcgaac caggctgtgc gatgcctcca cggccccggc ctcggtcctt
     3181 ccgagaaccg ccaccacctt cgcgccgtgt gcatggaggt acctggcgat gaacccgcct
     3241 atcccgttgt tcctgcgcaa cgcgc
//
LOCUS       DAOSCR010000289         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18821_length_3263_cov_2.378429, whole genome shotgun sequence.
ACCESSION   DAOSCR010000289 DAOSCR010000000
VERSION     DAOSCR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18821_length_3263_cov_2.378429"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLT69_07695"
     CDS             <1..660
                     /locus_tag="PLT69_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOJ14357.1"
                     /translation="LMKRLVFVAVVVSILAPLAKAEAGGPWRERHRYCPECVREGSRP
                     TGYLSLRIGAFSPDADSEVCGTGLALGGALGGRVNDNLAVEIGMEYAVADIDDRAAYR
                     LDYPADASVSTLGIPLSLKFIVPLAANVELWAGGGLGLYVSRLEFEDPFGEWYTDEDL
                     SDTDIGFHALVGADVALNPGIALTMELRHVETADEHGDDRFLVPDPSGTTAAFGVVFR
                     F"
     gene            complement(1309..>3263)
                     /gene="carB"
                     /locus_tag="PLT69_07700"
     CDS             complement(1309..>3263)
                     /gene="carB"
                     /locus_tag="PLT69_07700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ14358.1"
                     /translation="LEELRKRLAEPSSERQFLMYEALRKGMSVEELHQLTGIGRWFIQ
                     QMQELVALEEEILQYRGSLPPDDLLIRAKKDGFADKYLGQILGVPEASVRQRRLELGL
                     KQGWQAVPVSGADAAYYFSTYNAPDSVPVSPNKKKVMILGGGPNRIGQGIEFDYTCVH
                     AAFALRDLGYESIMVNCNPETVSTDYDTSDKLYFEPLTVEEVLAIYEKEKPEGVICQF
                     GGQTPLNIASDLAKAGVRILGTSPETIDLAEDRARFGAMMSKLGIPMPVSGMASTLEE
                     ALEVAGRIGYPLMVRPSYVLGGRGMEIVHDEEMLREYVKAAVDVTPERPILIDRFLDN
                     AIEVEADAISDGTDAFVPAVMEHIEQAGVHSGDSACVIPPISIPAKHLETIHEYTRRI
                     AVELGVVGLMNMQYAIHNDTVYVLEANPRASRTVPLVSKVCNVAMADIATRIILGKTL
                     KDFDLAKRHIPHFGVKEAVFPFPMFPEVDPLLGPEMRSTGEVLGMAHSFGLAFAKAAE
                     AASQTLPKEGTVLITVSRRERPAVLEVARLFHELGFKIKATRHTHFFLRDNNIPTEMV
                     LKLHEGRPNIVDGIKNKEYDLIINTPSGKLSKHDDSYIRKSAIRYKVPYITTLSAAIA
                     AARGIEELRKGRGQTRSLQEYHRDIT"
BASE COUNT          519 a         1007 c         1103 g          634 t
ORIGIN      
        1 ctcatgaaga ggctcgtgtt cgttgccgtc gtcgtatcga tcctggcccc cttggctaag
       61 gccgaggcgg gtgggccgtg gcgggagcgt caccggtatt gcccggagtg cgtgagggaa
      121 ggttccaggc cgacaggcta cctgtcgctc aggatcgggg ccttttcgcc cgatgccgac
      181 tccgaggtgt gcgggaccgg ccttgcgctc ggcggggcgc tcggcggcag ggtcaacgac
      241 aaccttgccg tggagatcgg catggagtac gccgtcgccg atatcgacga ccgggctgcc
      301 taccggttgg attacccggc ggacgcctcg gtgtccacgc tcgggatacc gctcagcctc
      361 aagttcatcg tccccctggc cgcaaacgtg gaactctggg cgggcggggg cctcgggctg
      421 tacgtttccc ggctcgagtt cgaagaccct ttcggcgagt ggtacacgga cgaggacctg
      481 agcgatacgg atatcgggtt ccatgccctc gtgggcgccg atgtcgccct gaacccgggc
      541 attgcactca ccatggagct gaggcacgtc gagaccgccg acgagcacgg cgatgaccgg
      601 ttccttgtcc ccgatccgag cgggaccacc gcagccttcg gcgtcgtgtt caggttttga
      661 agggttcaga gacgtcatca cggccgatac cgtgaaagag gggggcgtgg gtgtcggtcc
      721 gtccagcccc tcctttccgg aaagcgcctg gacggagagg cggtgtcggt gggcacggcc
      781 gaaacggtat cccaggggtt gtcctgtatc tccttcactg ttcttggctt gtgcggaagg
      841 caggccggag gctgcccgag tcttcccgcc ccctgagcgg tcacctgccg ggcttttccc
      901 tcatcaggaa cgatcggcac acggagtttc ttgccgcctg gccttgctcg ggtcgagggg
      961 gtcgacaaga gggaaagggg aggacgaggg gttgtacgtc tcgatccaac ctggtgagaa
     1021 atgggggtct cctgcgcagg attccaccca ttgctgaaaa tgcatgccca atgacaagcc
     1081 ggtcaagtaa acatgcagtt cattcggatc tcttcagctc tcagccccgt tcaagtccga
     1141 ttatgctgca caggagacgg ccttccctat actccacgcc ggggatcaaa tcaaccgaga
     1201 aaatccatga acccctgaga acctgacaac tcggcacgta tgcaacggcc tggggagcgc
     1261 ccgcggtctc tcatcccgag gcaacggggg ccgtaggcgg ccgggcattc acgtgatgtc
     1321 ccggtggtat tcctggagcg accgggtctg ccccctgcct ttcctgagct cctcgatgcc
     1381 cctggcggcc gcgatggctg cgctcagcgt ggtgatgtac ggcaccttgt agcggatggc
     1441 cgacttgcgg atgtacgagt cgtcgtgctt gctcagcttg ccgctcggcg tgttgatgat
     1501 gaggtcgtac tccttgttct tgatgccgtc cacgatgttg ggcctgccct cgtggagctt
     1561 gaggaccatc tcggtgggga tgttgttgtc cctcaggaag aagtgcgtgt ggcgcgtggc
     1621 cttgatcttg aaccccagct cgtggaagag cctcgccacc tccaggaccg ccggccgctc
     1681 ccgcctcgat acggttatga ggaccgtccc ctccttgggc agcgtttgtg aggccgcctc
     1741 cgcggctttc gcgaaggcga gcccgaagga gtgggccatg ccgagcacct ccccggtgga
     1801 gcgcatctcc gggcccagga gggggtcgac ctcggggaac atggggaagg ggaacaccgc
     1861 ctccttgacg ccgaagtggg gtatatgcct cttggccagg tcgaagtcct tgagggtctt
     1921 gccgaggatg atcctggttg cgatgtcggc catggccacg ttgcacacct tggagacgag
     1981 gggcacggtg cgggaggcgc gcgggttcgc ctcgagcacg tacaccgtgt cgttgtggat
     2041 cgcgtactgc atgttcatga gcccgaccac gccgagttct acggcgatgc gcctcgtgta
     2101 ctcgtggatg gtctcgaggt gcttggcggg gatgctgatg ggcggtatca cgcacgccga
     2161 gtcgcccgag tgcacgccgg cctgctcgat gtgctccatg accgccggca cgaacgcgtc
     2221 cgtcccgtcg gagatggcgt cggcctccac ctcgatggcg ttgtccaaga acctgtcgat
     2281 gaggatgggc cgctcggggg tcacgtccac ggccgccttc acgtactccc ggagcatctc
     2341 ctcgtcgtgg acgatctcca tgccgcggcc gccgaggacg taggaaggcc tcaccatgag
     2401 ggggtaaccg atacggccgg ccacctcgag cgcctcctcg agggtgctcg ccatgccgga
     2461 gacgggcatg gggatgccga gcttggacat catggcgccg aagcgcgcac ggtcttccgc
     2521 caggtcgatg gtctcggggg aggtgccgag gatcctcacg cccgccttag cgagatcgct
     2581 cgcgatgttg agcggcgtct gcccgccgaa ctggcagatc acgccctcgg gcttctcctt
     2641 ctcgtagatc gcgagcacct cttcgacggt caacggctcg aaatagagct tgtccgaggt
     2701 gtcgtagtcg gtggacacgg tctccgggtt gcagttgacc atgatcgact cgtagccgag
     2761 gtcgcgcagg gcgaaggcgg cgtgaacgca ggtgtagtca aactcgatcc cctggccgat
     2821 gcggttgggg ccaccgccga ggatcatcac cttcttcttg ttgggcgaaa ccggcacact
     2881 atcgggggcg ttgtaggtgg agaagtagta agcggcatcg gcgccgctga cgggcaccgc
     2941 ttgccaccct tgcttgaggc cgagctccag gcggcgctgg cgcacgctgg cttccggcac
     3001 gccaaggatc tggccgaggt atttgtcggc gaagccgtct ttcttggccc ggatcagcag
     3061 gtcgtccggg ggcagggagc cccggtactg gaggatctcc tcttccagcg ccactagctc
     3121 ttgcatctgc tggatgaacc agcgcccgat gccggtgagc tggtgcagct cctccaccga
     3181 catccccttg cgcagtgcct cgtacatcag gaactggcgc tcggaggagg gctcggccag
     3241 gcgcttgcgc agctcctcca ggg
//
LOCUS       DAOSCR010000290         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18893_length_3254_cov_3.221944, whole genome shotgun sequence.
ACCESSION   DAOSCR010000290 DAOSCR010000000
VERSION     DAOSCR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18893_length_3254_cov_3.221944"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLT69_07705"
     CDS             complement(<1..599)
                     /locus_tag="PLT69_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOJ14359.1"
                     /translation="MSVRPVMPEHLASRISTIATPAYVVDMAALMRNMETASLIRREA
                     GCRILLSTKAFALPEVFPLMREHLDGTSASGLYEARLGREEFGKEVHVFSPAYREETF
                     GEILGVCDHVTFNSPSQLERFLPAVEKSGKGVRAGIRVNPGYSRVTLGGELYDPCSPR
                     SRFGVRRHLLDVLPWDSLDFIHVHALCESLHDGSTALIE"
     gene            complement(929..2140)
                     /locus_tag="PLT69_07710"
     CDS             complement(929..2140)
                     /locus_tag="PLT69_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOJ14360.1"
                     /translation="MDSILIIGAGAAGSVAAKKCAMNRDVFAKIHLASRTLEKCLKVQ
                     KECATPITVSQVDADDVSQVVALIEEKRPDLVLNMALPYQDLPVMDACLETGVHYMDT
                     ANYEPRDEARYSYTWQWAYHDRFKDRGIMAVLGCGFDPGVISIFCAYAQRHLFDEIHY
                     IDIVDCNDGQHGKAFATNFNPEINIREVTQRGKYWKEGSWIEIDPMSVSCMIDYPEVG
                     PRKSYLIYHEEEESLVLNIKGLKQIRFWMTFSDNYLKHLEVLQNVGLTRIDPVVYKGV
                     EIVPLEFLKQLLPEPSSLAENYTGKTSIGAIMRGVKDGRQEACFMYNVCDHAQTYREI
                     GAQAVSYTTGVPAATGAMMVMKGLWSGTGVFNVEQLPPEPFLEELARQGLPWHVRKID
                     PRSYKELFETA"
     gene            complement(2193..3017)
                     /gene="speB"
                     /locus_tag="PLT69_07715"
     CDS             complement(2193..3017)
                     /gene="speB"
                     /locus_tag="PLT69_07715"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOJ14361.1"
                     /translation="MNSFLASKPEGRIVLFGAPFDSTSSYRPGSRFGPQAIREASHSL
                     ETFSPEQLLDLEECPFTDDHDLELPFGDPCPALDLIEQKAARVISHGGVPFLLGGEHL
                     VSLGAIRAASSMYPNLRVVHLDAHADLRDEYLGMRLSHATVMRRVLDLVGQDRLHQIG
                     IRSCTREEHELSSRLSTTPEKVSLLAGENPCYLTCDIDILDPSVLPGTGTPDPGGLSF
                     RELSNLLVSMITRLRVVGMDIVELSPGLDPSGVSAVVAAKLVRESLLALHRAPACG"
     gene            complement(3053..>3254)
                     /locus_tag="PLT69_07720"
     CDS             complement(3053..>3254)
                     /locus_tag="PLT69_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HOJ14362.1"
                     /translation="TDEDHAGLIMEYSASDTKEHVEEKVRKMAEEGMKMRGKEVREIK
                     SVAVEHTVKACGAAFAAVVLWD"
BASE COUNT          542 a         1017 c         1054 g          641 t
ORIGIN      
        1 tgctcgatga gggctgtcga gccgtcatgc agggactcac acagggcgtg tacgtggatg
       61 aagtcaaggg aatcccacgg caggacatcg aggaggtgcc gcctaacccc gaagcgggac
      121 ctgggcgaac acgggtcgta gagttcgccc ccgagggtga ccctcgagta gccggggttc
      181 acccggatgc cggcacggac gcctttcccg ctcttttcga cggccggcag aaaccgctca
      241 agctgggagg gggagttgaa ggtgacgtga tcgcacacgc ccaggatctc gccgaaggtc
      301 tcttccctgt aggccgggga gaagacgtgc acctctttcc cgaactcttc cctgccgagc
      361 ctggcttcgt agagaccgct cgcgctcgtg ccgtcgaggt gttcgcgcat gaggggaaag
      421 acctcgggga gggcgaacgc cttggtcgag aggaggattc tgcagccggc ctcgcgcctg
      481 atcagggacg ccgtctccat gttgcgcatg agcgcggcca tatccaccac gtaggcgggt
      541 gtggcgatcg tcgagatccg ggaggcgagg tgttccggca tgaccggtcg gacagacacg
      601 gcaccccccg taatcagaag gccttcaggg gcggcctccg cctggtcggc agcggcctgt
      661 ccagggggtc ctccctgccg gggcctcctg tctcacggcc ctgcaggcaa aggcagtggt
      721 aagggctgct cgtgaaatgg accggtcccc agcgagactc gtgggccttg ttcgagctgc
      781 gagaccatgt gctgccgcaa cagggaagag gacacccttt tcgccttcct cggggcccgg
      841 tcgaacagat cgagagacgt gcctgagctc gggcttgctc atcgatgcgc atcccatgca
      901 tgcgaggtca cctcgtcaag gattgccgct acgcggtctc gaagagttct ttgtacgagc
      961 gggggtcgat cttcctcacg tgccaaggga gcccctgcct ggcgagttcc tcgaggaacg
     1021 gctcgggcgg gagctgctcc acgttgaaca cccccgtgcc gctccagaga cccttcatga
     1081 ccatcatggc gccggttgcg gccggcacgc cggtcgtgta cgagacggcc tgggccccga
     1141 tctcgcggta ggtctgtgcg tgatcgcaca cgttgtacat gaagcaggcc tcctgcctgc
     1201 cgtccttgac ccccctcatg atggcgccga tcgaggtctt gcccgtgtag ttctcggcca
     1261 gcgacgacgg ctcggggagg agctgcttga gaaactccag gggcacgatc tccacgccct
     1321 tgtagacgac ggggtcgatc ctggtgagcc ccacgttctg caagacttcc aggtgcttca
     1381 agtaattgtc ggaaaaggtc atccagaagc gtatctgctt gaggcccttg atgttcaata
     1441 cgagcgattc ctcctcctcg tggtatatga ggtaagattt cctcgggccc acctccgggt
     1501 agtcgatcat gcacgagacg ctcatcgggt cgatctcgat ccagctgcct tccttccagt
     1561 actttcccct ctgcgtaacc tccctgatgt tgatctcggg gttgaagttc gtcgcgaagg
     1621 ccttgccgtg ctggccgtcg ttgcagtcca cgatgtcgat gtagtgtatc tcgtcgaaga
     1681 ggtgtctctg tgcgtaggcg cagaagatgc ttatcacgcc cgggtcgaaa ccgcacccga
     1741 gcaccgccat gatgccacgg tccttgaatc ggtcatggta ggcccattgc caggtgtagc
     1801 tgtacctcgc ctcgtccctc ggctcgtagt tggcggtgtc catgtaatgt acgcccgtct
     1861 cgaggcatgc atccatgacg ggcaggtcct ggtagggaag ggccatgttg agcacgaggt
     1921 cgggtctttt ctcctcgatg agggccacga cctgcgaaac gtcgtcggcg tccacctggg
     1981 acacggtgat cggcgtcgcg cattctttct gcaccttgag gcatttctcc agggtcctgg
     2041 atgcgaggtg gatcttggcg aacacgtcgc ggttcatggc gcacttcttc gccgcgacgc
     2101 tgcctgcagc gcctgcgccg atgatgagga tgctgtccat cgtcttcctc ctgcttcgga
     2161 tattctcttg ggttgtaccc cggcgatgag gctcagccgc acgccggcgc cctgtgaagc
     2221 gccagcaggc tctcccggac gagcttcgcg gcgacgacgg cggagacgcc cgacgggtcg
     2281 agcccgggcg acagctccac gatgtccata ccgacgaccc tgagcctggt tatcatggac
     2341 acgagcaggt tcgagagctc cctgaacgac agccctccgg ggtccggcgt ccccgtgccg
     2401 ggcaacacgg aagggtccag gatgtcgatg tcgcaggtga ggtaacacgg gttctcgccg
     2461 gccaagaggg agaccttctc gggcgtcgtc gacagcctcg aggagagctc gtgttcttcc
     2521 ctggtgcacg accgtatccc gatctggtgg agccggtctt gtcctacgag atcgagcacg
     2581 cgcctcatga cggtcgcatg ggagagcctc attccaaggt actcgtccct gagatccgcg
     2641 tgagcgtcga gatggaccac cctgaggttc gggtacatgc tcgaggctgc ccgtatggcc
     2701 cccaggctca cgaggtgttc cccgccgagc aggaaaggga caccgccatg cgagatcacc
     2761 cgggccgcct tctgctctat gagatcgagg gccgggcaag ggtcgccgaa ggggagctcg
     2821 aggtcgtgat cgtcggtgaa cgggcattcc tcgaggtcga ggagttgttc cggcgagaag
     2881 gtctcgaggg agtgcgacgc ctcgcggatg gcttgcgggc cgaagcgtga gcccggccgg
     2941 tagctggagg tggagtcgaa gggcgcaccg aagagtacga tccgcccttc gggcttggat
     3001 gcgaggaacg agttcacctt cgcttgccgc accggttcgg ggacgagccg cgctaatccc
     3061 agagcaccac ggccgcgaag gccgcgccgc aggccttcac ggtgtgctcc acggccacgc
     3121 tcttgatctc gcgcacctcc ttgcccctca tcttcatccc ttcctccgcc atcttccgca
     3181 ccttctcctc gacgtgctcc ttcgtgtcgc tggcggagta ctccatgatg aggcccgcgt
     3241 ggtcttcgtc agtg
//
LOCUS       DAOSCR010000291         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18947_length_3249_cov_3.541327, whole genome shotgun sequence.
ACCESSION   DAOSCR010000291 DAOSCR010000000
VERSION     DAOSCR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18947_length_3249_cov_3.541327"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   65..246
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=65..100
                     /rpt_unit_seq="atttccacgtctgaatagacgtggccccattgaagc"
     gene            509..1405
                     /locus_tag="PLT69_07725"
     CDS             509..1405
                     /locus_tag="PLT69_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ14363.1"
                     /translation="MHETTDAHRAQRLKIAIGLTASTLVAEVAGGLWTNSLALLSDAA
                     HVFLDLFALVLSLAAIRLAMLPANEEHSFGWHRAEVFASFINGVTVFLMAAAIFYEAL
                     KRLVSPQEVDSLPMFIIAAVGLVANGLSAVALHTHSHDDLNVRSAFVHVIGDAAASVG
                     VIIGGIIMYVTRWYVLDALISMGIGIIIFTGAFRVLRESVHILMEGVPRGMNITEVAE
                     TIRGVKGVRDLHHLNVWAICSHITALSVHLDIDPGSGQEASAILRDIERVLLERHNIT
                     HTTLQIECTACANGPVIKEFSH"
     gene            1763..2566
                     /locus_tag="PLT69_07730"
     CDS             1763..2566
                     /locus_tag="PLT69_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOJ14364.1"
                     /translation="MADPKGDEIRKAVRENYGEVARAAGTGCCSTPSCCGGARVVSME
                     DMASGLGYSRDDISAAPEGSFMGLGCGNPIEAAAIQPGETVLDLGSGGGFDCFLAARI
                     TGDAGLVIGVDMTPEMVARSRENARKAGVRNVEFRLGELENLPVADGVVDVIVSNCVI
                     NLSPDKERVFRETFRVLKPGGRIAISDIVSTAALPEEVRRDLAMHVACIAGATPVGEL
                     ESMLAGAGFVDVRVTVKEESRTFIKEWVPGSRVEECVVSAIIQAVKPRG"
     gene            complement(2741..2834)
                     /locus_tag="PLT69_07735"
     tRNA            complement(2741..2834)
                     /locus_tag="PLT69_07735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2798..2800),aa:Ser,seq:gct)
     gene            3055..>3249
                     /locus_tag="PLT69_07740"
     CDS             3055..>3249
                     /locus_tag="PLT69_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14365.1"
                     /translation="MDRTLTRAMPALAALVAALVVSGCASWSQAGKAEGVPAAPAAQV
                     KPLGVFGARVELHGEDPFILR"
BASE COUNT          596 a          999 c         1112 g          542 t
ORIGIN      
        1 acaaactgct cctcaaaatg ttacgatcta accccctaaa gttgtcatga accttgaaaa
       61 atatatttcc acgtctgaat agacgtggcc ccattgaagc taatcatatg tgcgcggccg
      121 ccattgcttt gcgaagattt ccacgtctga atagacgtgg ccccattgaa gcttgatctc
      181 gtcagggaga tcaaggaact tgtcagccga tttccacgtc tgaatagacg tggccccatt
      241 gaagccgctg ttcgtgtagc tttatagacg cattgccata tagggcatgg gagtgacagc
      301 agcatcacac ccgacgaagg ccatggcctg cctgcgtggc aagaatgggc ataacggcgg
      361 cggggaagat tgctggttgt gagaaccctt cattgtgtgg ttatcatcat tgaacgaaga
      421 gggaaaaagc cggtcatgga cgagagagcg gacatcgacg ggtcgactga ccacaccgtg
      481 ttccccaacc aaccggtaga cgcctgccat gcatgagact acagacgcac acagggcgca
      541 gcgcctgaag atcgccatcg ggctgaccgc atcgacgctc gtggccgagg tggcgggagg
      601 tttgtggacc aactcgctgg cactgctctc ggacgccgcg cacgtctttc tggacctctt
      661 tgcgctcgtg ctgtcgctgg ccgcgatcag gctcgccatg ctccccgcca acgaggagca
      721 ctccttcggc tggcaccgcg ccgaggtgtt cgcctcgttc atcaacgggg tcacggtctt
      781 tctcatggcc gcggcgatct tctacgaggc gctgaagcgc ctggtctcgc cgcaggaggt
      841 ggacagcctc ccgatgttca tcatcgccgc cgtcgggctc gtcgcgaacg ggctttccgc
      901 cgtggcgctg catacccact cccacgacga cctgaacgtg cggagcgcct ttgtccacgt
      961 gataggggac gcagccgcct cggtgggcgt gatcatcggc ggtatcatca tgtatgtcac
     1021 ccggtggtac gtgctcgacg ccctcatatc catgggcatc ggcatcatca tattcacggg
     1081 cgcgttcagg gtgctcaggg aatccgtgca catcctcatg gagggtgtgc ccagggggat
     1141 gaacatcacg gaggtggccg agacgatcag gggcgtcaag ggggtgaggg acctgcacca
     1201 cctgaacgtg tgggcgatct gctcgcacat cacggcgctg tcggtgcacc tggacatcga
     1261 cccgggcagc ggtcaagagg catccgccat tctccgggac atcgagcggg tgctgctgga
     1321 gcggcacaac atcacgcaca ccaccctgca gatcgagtgc accgcgtgcg ccaacggccc
     1381 cgtgatcaag gagttcagcc attgacgggc cggacccggg ccggctgaaa gatgcgaggg
     1441 tgaaagcggg gtttccttcg ccggcagggg tgaggtatct tcttgccgga gaaagagcgg
     1501 cgtgggatac aaggctgtgc caggcgccgc atgggccggc ggtctcccct gcggcgcctg
     1561 gtgaggtcta agggacgagg ggcgcccttg ccaggcatgc ctcggggatg cctgactgga
     1621 gagcgccagg cagaggatgt cccttcggtg atgcaggcct tcgggcccca accggcgcct
     1681 gcacagacga acgcgaagga acatggcaag agaccggccc cggctgtggg agcggggcgg
     1741 tcaggaaaag gaggtacgta ccatggcaga ccccaaaggc gacgagatcc gcaaggctgt
     1801 ccgcgagaac tacggcgagg tggccagggc cgccgggacc gggtgctgtt ccaccccctc
     1861 ctgctgcggg ggcgcgcggg tggtctccat ggaagacatg gcgtccgggc tcggctactc
     1921 gagggatgac atctcggcag cccctgaagg ctcgttcatg gggctcggct gcggcaaccc
     1981 gatcgaggcc gcggccatcc agcccgggga gaccgtgctc gacctgggca gcggcggagg
     2041 cttcgactgt tttctcgccg cgcgtatcac aggggacgcg ggcctggtga taggcgtgga
     2101 catgaccccg gagatggtcg caaggtcgcg cgaaaacgcc cgcaaggcgg gcgtgcgaaa
     2161 cgtggagttc aggctgggcg agctggaaaa cctccccgtc gcggacggcg tggtcgacgt
     2221 catcgtctcg aactgcgtga tcaacctgtc cccggacaag gagcgggtgt tcagggagac
     2281 cttccgcgtc ctgaagccgg gcggcaggat cgcgatctcc gacatcgtga gcacggccgc
     2341 gcttcccgag gaggtgaggc gggacctggc catgcacgtc gcctgcatcg cgggcgccac
     2401 gccggtcggt gagctcgagt ccatgctggc cggggccggc ttcgtggatg tgcgggtcac
     2461 ggtgaaagag gagagcagga ccttcatcaa ggagtgggtg cccggcagcc gggtggagga
     2521 gtgcgtggtc tcggccatca tccaggccgt caagccccgc gggtaggggc gcaaggggcg
     2581 gccgccccgg gcgtgcgcga gggccgaaga agaccgcggg acgcgtatgc tcgggcgggt
     2641 ctctgccctt cgtcaccgga tgccggatcg ccctggtgca gacagcgccc ggtcaggccg
     2701 cccgtacccg gtgggcaacg aggccctcat cgcagacgat tggcggagag agagggattc
     2761 gaaccctcgg tagagctttt tggccctaca ttcgcttagc aggcgaacgc cttcgtcctc
     2821 tcggccatct ctccaccggc ccacggcaga aggggccatg cccgcagccg tgaacgtcac
     2881 atcataacta cacaaggcgg cggcgagtca agtacgcagt gtgggcgctc cctggccgcc
     2941 cgggcacgag cccgcaaccc cgtgcacgcg gcctcagcgg tatcgcgctc gagtcgctcg
     3001 cagcgcagca gccgggggtt gacgcgaagg cggaaaggtc gcacaaaggg acacatggac
     3061 cggacactca caagggccat gcccgccctg gcggcgctcg tggccgccct tgtcgtctcg
     3121 gggtgcgcct cgtggtccca ggccgggaaa gccgagggag tacccgccgc gcccgccgcc
     3181 caagtgaagc cgctcggcgt cttcggtgcc agggtcgagc tgcacgggga ggaccctttc
     3241 atcctgagg
//
LOCUS       DAOSCR010000292         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18990_length_3244_cov_2.927250, whole genome shotgun sequence.
ACCESSION   DAOSCR010000292 DAOSCR010000000
VERSION     DAOSCR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18990_length_3244_cov_2.927250"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PLT69_07745"
     CDS             <1..1299
                     /locus_tag="PLT69_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14366.1"
                     /translation="RLPQTLHEERIKEDGREIFDVIVYPLVGGKQGGVVFTAVDITEK
                     KEMELQLVHAQKMETIGELAGGVAHDFNNIFTGITGNVSMLRFTTDEEKRQQYLKSLE
                     NITERARGLTRQILAFSKRNEGNPEVILVDSAVREVMDISTKSIPKNVRIEYTSSGKD
                     LSVFMDHTHLVQVLLNLVVNAKDAIGSNQDGRISISVSPMLVDKESRRQYLLGSIGRH
                     VRIEVADNGCGMDMSVLPKIFDPFFTTKQKGPNKGTGLGLSIAYNIIKNAGGSIQVSS
                     TPGKGSTFTVLLPVAKARDKSKDHETRPMQTAARQSARILMVDDEDMLRDIGKDMLCM
                     LGHSVTTATNGRECIDILKNDPDGFDLVILDMIMPELDGYHTLQQMKALGLSTRVIIS
                     SGFSFEHEKEDFLENPLIVARLNKPFNLEELSQVLDEALS"
     gene            2190..>2736
                     /locus_tag="PLT69_07750"
     CDS             2190..>2736
                     /locus_tag="PLT69_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14367.1"
                     /translation="MRETVVFDGAFYEELFENSAAIGAVTDANGVILRVNRRALDLFF
                     GTNGTVPDVIGRNIVEFIHKEDLPKVFENWKKSLREHKEVSYELRMCSPDGRVMYFLI
                     SGRPIVKEGKVALFHYQALNMLDLKVHEQNLMASASADVIAQIAGGFAHDFNNLLTVI
                     NGYAQMMRMSLDASSPFHHKVN"
     assembly_gap    2737..2746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2747..>3244
                     /locus_tag="PLT69_07755"
     CDS             <2747..>3244
                     /locus_tag="PLT69_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14368.1"
                     /translation="ELTQRMLDFSKQTKTRHKLVDISAEISNQESIIRHILGERVSMT
                     VEKQPGLGKVMMDPGRVSTILVNLAANARDAMPDGGTFKLCADQFDAQTAFMLGKNQV
                     PAGRYIRLTVEDTGAGMDDAVFARIFEPFFSTKEQGKGLGLWTVKNIVSSCGGYLHVE
                     SVKGRG"
BASE COUNT          672 a         1045 c          966 g          551 t
ORIGIN      
        1 cgcctccccc agacgctcca cgaggagagg atcaaggagg acgggcgcga gatcttcgac
       61 gtcatcgtgt accccctcgt cgggggcaag caggggggcg tcgtgttcac cgccgttgac
      121 atcacggaaa agaaggagat ggagctccag ctcgtgcacg cccagaagat ggagaccata
      181 ggagagctcg caggcggcgt cgcccacgac ttcaacaaca tattcaccgg gatcacgggg
      241 aacgtctcca tgctcaggtt caccaccgac gaggagaaga ggcagcaata cctcaagtcc
      301 ctcgagaaca tcaccgagag ggcccgcggc ctcacgcgcc agatcctggc cttctcgaag
      361 cgcaacgagg gaaaccccga ggtgatcctg gtggacagcg ccgtccgcga ggtcatggac
      421 atatcgacca agtccatccc caagaacgtg aggatagagt acacctcctc gggcaaggac
      481 ctcagcgtgt tcatggatca cacgcatctc gtccaggtcc ttctcaacct cgtggtcaac
      541 gcgaaggacg ctatcgggtc gaaccaggac gggcggatat ccatctccgt gagccccatg
      601 ctggtggaca aggaatcgag gaggcagtac ctgctcggct ccattggaag acacgtgcgc
      661 atcgaggtgg cggacaacgg gtgcggcatg gacatgagcg tgctccccaa gatcttcgac
      721 cccttcttca cgaccaagca gaagggtccg aacaagggta cggggctggg cctctcgatc
      781 gcgtacaaca tcataaagaa cgctggtggg agcatccagg tgagttcgac ccccggcaag
      841 ggctccacct tcaccgtgct gctgccggtc gcgaaggcaa gggacaagtc caaggatcat
      901 gaaaccaggc cgatgcagac cgcggccagg cagagcgcca ggatcctcat ggtggacgac
      961 gaggacatgc tcagggacat cgggaaggac atgctgtgca tgctgggcca cagtgtcacc
     1021 acggccacga acggccgcga gtgcatcgat atcctcaaga acgacccgga cgggttcgat
     1081 ctcgtcatcc tcgacatgat catgcccgag ctcgacggtt accacacgtt gcagcagatg
     1141 aaggccctcg gcctctcgac acgcgtcatc atctcctcgg ggttctcctt cgagcacgag
     1201 aaggaggact tcctcgaaaa cccgctcatc gtcgcgaggc tcaacaagcc tttcaacctc
     1261 gaggaactct ctcaggtgct cgacgaggcg ctctcctgag gccttccttc cctttttccg
     1321 cccaggcgcg aggcctttct cgatgtcacg ggaggttctt ccgagacacc tggtgctgat
     1381 actcgggata accggagcga ggaggcgcct tttcttgctg tcacgggagg tacttccgag
     1441 accctgtgcc cttcaccttg ccgggcgtgc ttaaccgtcg ggaaaaggcc gccggcagca
     1501 ctccccctga gaccgtcttg tccgccctcg tacccgggtg gcacggacac cagggaaacc
     1561 acctggcggc agcattgtcg caactcgttg aagctggggg agtcgttgag cctcgagggg
     1621 accaggaagc caccgggcgg cagcattgtc gcaccccgtt cgacctccgt cccacccggc
     1681 tgtctcgagg ctcacccacc ccaagggccg ggcgcacgta cggcgccggg caccctgggt
     1741 gccgtgaccc gaacccttga tcgctccata tcccctttcc cctcgcatgg gggtcctgcc
     1801 cctaaggtct cgtaagcgta gcggcggcat ccaaggctct gcccgaaggg gtgcgcctcg
     1861 gggtgaccca agccgtgacc gggtgaagga ctttacctcg aacgaccggg acgtcacgtg
     1921 gtgcacctca aggtccccca ggcctcggcc ggggacgcga ccaccatctc tcctcgcgac
     1981 cctcgtcctc tcgtaggcct tcctcacggg agtagagcct cggtgggaag cccaccgtcc
     2041 accccttggc tcacacccct cgtcctctcg aaggcctgtc tcacccgagc cccgccgtca
     2101 agagacattg ctgtttattt cggcggcccg tctgccgata ggatctgtac gaacctgttg
     2161 gatcaatcac gcatgggggc atccaggtca tgagagaaac cgttgtcttc gacggcgcct
     2221 tctacgagga gctcttcgag aactccgccg ccatcggcgc cgtcaccgac gcgaacgggg
     2281 tcatcctcag ggtgaacagg cgggccctgg atctcttctt cggcaccaac gggaccgtcc
     2341 ccgatgtcat aggccgcaac atcgtggagt tcattcacaa ggaagacctg ccgaaggtct
     2401 tcgaaaactg gaagaagagc cttcgcgagc acaaggaggt gagctacgag ctccgcatgt
     2461 gctcgcccga cggcagggtc atgtacttcc tcatcagcgg aaggcccatc gtgaaggaag
     2521 gcaaggtggc cctgttccac taccaggcac tgaacatgct cgatctcaag gtccacgagc
     2581 agaacctcat ggcgtccgcg agcgcggacg tgatcgctca gatcgcaggc ggtttcgccc
     2641 acgacttcaa caacctgctc acggtcatca acgggtatgc ccagatgatg aggatgagcc
     2701 tcgacgcgtc atcgcccttc caccacaagg tcaaccnnnn nnnnnngagc tcacgcagcg
     2761 gatgctcgac ttcagcaagc agacgaaaac gagacacaag ctcgtggata tctccgcgga
     2821 aatctccaac caggaatcca tcatcaggca catcctgggc gagagggtat ccatgacggt
     2881 ggagaaacag ccgggcctgg gcaaggtcat gatggacccc gggcgggttt ccaccatcct
     2941 cgtgaacctg gcggcaaacg cccgggacgc gatgcccgac ggcgggacct tcaagctgtg
     3001 cgcggaccag ttcgacgccc agacggcctt catgctcggc aagaaccagg tgccggccgg
     3061 caggtacatc cgcctgaccg tggaggatac cggcgccggc atggacgatg cggtctttgc
     3121 gcgcatcttc gaaccgttct tctccaccaa ggagcagggc aagggcctgg gtctgtggac
     3181 cgtgaagaac atcgtgagct cctgcggcgg ctatctccat gtcgagtccg tcaagggcag
     3241 gggc
//
LOCUS       DAOSCR010000293         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_18993_length_3243_cov_2.746863, whole genome shotgun sequence.
ACCESSION   DAOSCR010000293 DAOSCR010000000
VERSION     DAOSCR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18993_length_3243_cov_2.746863"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(532..951)
                     /locus_tag="PLT69_07760"
     CDS             complement(532..951)
                     /locus_tag="PLT69_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOJ14369.1"
                     /translation="MTPPVQSATFSTMADKESGPNGSLDLQRVLARITDFDEMVSFCE
                     EIFTPRELKDLELRWKLLTELHRGTSQRSIASLYRISLCKITRGSKILKKKDSTARKI
                     LDEMYGRPASRRDKETSGPREAGSGGGADRKRRPRGR"
     gene            1147..1902
                     /locus_tag="PLT69_07765"
     CDS             1147..1902
                     /locus_tag="PLT69_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOJ14370.1"
                     /translation="MKIVSWNVNGLRAALKNGFAESVMSMGADVLCIQETRLQAPEPV
                     KGLEGYTPFYNFARKKGYSGTAVFTSVEPLSVSYGMGSAEHDDEGRIITLDFGGYYLV
                     NVYTPNAQHGLVRLDYRMRWDACFLEYLLELERTKPVVFCGDLNVAHKEIDLKNPKAN
                     ERNPGFTREEREGFDRILAHGFIDTFREFNKEPGNYTWWSYRFNARAKNIGWRIDYVC
                     ISASLRPSLVDAFILKDIYGSDHCPVGVVMDLS"
     gene            complement(2240..2668)
                     /locus_tag="PLT69_07770"
     CDS             complement(2240..2668)
                     /locus_tag="PLT69_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOJ14371.1"
                     /translation="MTRVLFVSLKGTARSRIARAWFEHLCPRGFTAASAGIRPGAAVD
                     PLAAQVMAEVGIVLEDEKPLSVYDIYRSREVFSRVVTVCSSADARECPVFLGLVRQEH
                     WAVKDPRAHAATDTKLLEGFRRIRDEIRELVAGLCDALQG"
     gene            complement(2715..>3243)
                     /locus_tag="PLT69_07775"
     CDS             complement(2715..>3243)
                     /locus_tag="PLT69_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD-binding family oxidoreductase"
                     /protein_id="HOJ14372.1"
                     /translation="GGVAGLLGEIARLVIEPGTPVKCLGPNGTFCRDIDDRPSVVLIA
                     GGVGITPFLSVLRHFHAKGASNEVMLFWSNKTFEDVFCLEEISGMSNALNLKVVHNLS
                     REDDPKRHLRGGYPNVVFESGRLDAGVLSRYGVNPKAAFYLCGPPPMMDSVLQELSTL
                     GVDPEAVEREKFSWK"
BASE COUNT          577 a         1088 c          992 g          586 t
ORIGIN      
        1 cacagccggc acgacgcctc gactcgctgg cgattccaag ggcgaccttg atggtcctac
       61 tcggtcttga cacacagcag acacgacgcc ttgactctgg cgtggccggt attatcattc
      121 agggcaagcc tcgaggctgc atttcgaagg gaaaaacccc atagaccctc ggtcgaaatg
      181 tcctcggggc ttttttcagg cagggacgag gagaccgtgc ggccggcccg cgcgtgcaga
      241 cggcaatcat cggcgcctca aggccggccg taagcccccg cgcttcggcg ccgtggatgc
      301 agtgtgggtt ttcccatgcc ccgcctgggt tccccgtagg cggaaggaat cagccgatca
      361 caatctatcc tcggcggcga ctgtgacggg ttacccgcag gcggaaggaa tcagcgcctc
      421 ctaggtgttg ccggacgatc gggcacccat gcccgcacgg cacagaggcc gggcgtgggc
      481 tttgcttccc cttggccgtc gttcagcccc ccacccgggg tgcgcgccgt gtcaccttcc
      541 cctcggacgt cttttccggt cggcgccgcc tccgcttcct gcctcgcggg gtccgctcgt
      601 ttccttgtcg cgcctgctcg caggccttcc gtacatctcg tcgaggatct ttctggccgt
      661 ggaatccttc tttttcagga tcttcgagcc cctcgtgatc ttgcagaggc ttatgcgata
      721 cagcgaggcg atgctcctct gggacgtgcc ccggtggagc tctgtgagga gcttccacct
      781 gagctcgagg tctttcagtt ccctcggcgt gaagatctcc tcgcaaaagg aaaccatctc
      841 gtcgaaatcg gtgatgcgag ccagcaccct ctgcaggtcg agggaaccat tcggcccgga
      901 ttccttgtcc gccatggtgg aaaaggtagc cgactgtacc ggtggtgtca aaacctttgt
      961 aaaagaagcc caccgacagc ccccgggata tccatacccg aactcccgcc gggctccgta
     1021 cacaaaacga ggaacagccg ggtcaccctc gcccggcata gcgcttcccg gagacggcca
     1081 tcgactcctt gagccggggc cccccctggt gtatgagtag ttccacgtgc atgtcgcgag
     1141 gtggccatga agatcgtctc ttggaacgtc aacgggctca gggcggcact gaagaacggc
     1201 ttcgccgaga gcgtcatgag catgggcgcc gacgttctct gcatccagga gacccgactg
     1261 caggcgcctg agccggtgaa gggtctcgag ggctacaccc ctttctacaa cttcgcacga
     1321 aagaagggtt actcggggac cgcggtgttc acctccgtcg agcccctgtc cgtgtcctac
     1381 gggatgggga gcgcggagca cgacgacgag ggccgcatca tcaccctcga tttcggcggg
     1441 tactacctcg tcaacgtcta cacacccaac gcccagcacg gcctcgtgcg gctcgactac
     1501 cgcatgcgct gggacgcctg tttcctggag tatctcctgg agctcgagcg caccaagccc
     1561 gtcgtcttct gcggcgatct caacgtggcc cacaaggaga tcgacctcaa gaacccgaag
     1621 gccaacgagc gcaacccggg gttcacgagg gaagaacgcg aagggttcga caggatcttg
     1681 gcccacggct tcatcgacac gttcagggag ttcaacaagg agcccgggaa ctacacgtgg
     1741 tggtcgtacc gcttcaacgc gagggccaag aatatcggct ggcgcatcga ttacgtgtgc
     1801 ataagcgcct ccctgaggcc ttccctggtg gacgcgttca tcctcaagga catctacggg
     1861 tcagaccact gccccgtggg cgtggtcatg gacctttcct gagaaaaaag cgcggctgga
     1921 gacgaaggcc gtgcccatgt ccgcctgcgg gtgcgcctga gagcctgccg acacggtgcg
     1981 atgcgcagct cccgagtgca ccggcccttc atgactgcgg tcgcgcccgg caggacatgc
     2041 accttttttc gcctcgagcc ggccgcgaag ggccctgtgc gacgggccac ggcacacgca
     2101 ggcatgcaag gccgcacccg tgcacgccgg gcaaggcgca cgtccgcatc gtcccattcc
     2161 aggaatgagc gtgccgtcct gaggttcgag gaggccggga aacgtctcgg ccaggcgcag
     2221 gcttgcccgg cggcgagggc tatccttgca gagcgtcgca caggccggcg acgagttcac
     2281 ggatctcgtc cctgatacgc ctgaagcctt ccaggagctt ggtgtccgtg gcagcgtgcg
     2341 cgcgcggatc cttcaccgcc cagtgctcct gcctcacgag cccgaggaac accgggcatt
     2401 ccctggcatc tgcgctcgag cagaccgtga caaccctcga gaagacctcc ctcgaacggt
     2461 agatgtcgta cacgctcaag ggtttctcgt cctcgaggac aatgcccacc tcggccatga
     2521 cctgtgcggc gagcgggtcg acagcggcgc ctggccttat gcctgcgctc gcagcggtga
     2581 acccccgggg gcagaggtgt tcgaaccagg cccgggcgat ccggcttctc gcggtaccct
     2641 tgaggctcac gaacaggacg cgggtcatca cgatacctgg gccggcctgg ctccctccat
     2701 cgccctgggc cgcatcactt ccagctgaac ttctcgcgct cgacagcctc ggggtccacg
     2761 ccgagggtcg acagttcctg gaggaccgag tccatcatgg gcggcgggcc gcacaggtag
     2821 aacgccgcct tgggattcac cccgtatcgg gaaagcacgc ctgcatcgag ccgcccgctc
     2881 tcgaacacca cgttcgggta cccgcccctg aggtgtctct tggggtcgtc ttcccggctc
     2941 aagttgtgaa ccaccttgag gttgagggca ttgctcatcc cggatatctc ctcgaggcag
     3001 aacacgtcct cgaaggtctt gttcgaccag aacagcatca cctcgttcga cgcccccttg
     3061 gcatggaagt gcctgaggac gctcagaaac ggggtgatgc ccacaccgcc cgcgatgagg
     3121 acgaccgatg gcctgtcgtc gatatccctg cagaacgtgc cgttcggtcc caagcacttc
     3181 accggcgtgc ccggctcgat gaccagccgt gcgatctcgc cgagcagccc ggcaacgccg
     3241 ccg
//
LOCUS       DAOSCR010000294         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19008_length_3241_cov_3.020088, whole genome shotgun sequence.
ACCESSION   DAOSCR010000294 DAOSCR010000000
VERSION     DAOSCR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19008_length_3241_cov_3.020088"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLT69_07780"
     CDS             <1..814
                     /locus_tag="PLT69_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ14373.1"
                     /translation="PVGKNTAPCIGFAAVVISRKDPDATMAVFPSDHLIREPERLKEA
                     ILFGVSALASHPDLLITLGVVPDRPETGYGYIAPETVLLSRGGLTIKRVRAFHEKPAR
                     IVAEQYVKDGYLWNAGMFLWRVDTILEMFSRHMPELHEQLQELKATMDSDASAVDRFF
                     NEAQSVSIDYGIMEKAGRVAVIPVDIGWSDVGTWDAMGNLYVTDDRGNSVRGPAELIN
                     SENNVIWSTDKRIVLIGVSDLVVVEGSDSILVCPRALSQEVSVVAKKLGDGK"
     gene            1522..2271
                     /locus_tag="PLT69_07785"
     CDS             1522..2271
                     /locus_tag="PLT69_07785"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HOJ14374.1"
                     /translation="MRIEILGCSGSVLSGFDTMSILVDGKILLDAGSVMSTIGVDRAT
                     GIRTVLLTHPHIDHIKELPFLVDALYTHKAKGIRIIGSPQTIDALKAHIFNGLIWPDI
                     AELDASSDFISLEHVPGGVFEIEGMEVKAFPTNHTGGAYGYVITENARSVLFSGDTGY
                     HPGLFELVGELGSNLAACFIEASFPSRMEDLARITQHLTPSLVAKGLDGVAAPGTRVI
                     LYHIKPAYLDEVIAELPRGFEYVRGGEVFVV"
     gene            2268..3023
                     /locus_tag="PLT69_07790"
     CDS             2268..3023
                     /locus_tag="PLT69_07790"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HOJ14375.1"
                     /translation="MTDILEIAKKDGRWTECPKCSEIVITEKLRQNLWVCQHCGYHFR
                     VNAPTRIEMTCDEGSFVESMPPVRDQEETAGSHDEAITCGRARIMGRECAIGVMDFFH
                     KGGSMGAAVGEAIVSLMQAASRKRLPIVMFCASGGVRVQEGIWGLFQMLRTVHARTRT
                     SDTPMITVFTDPTTGGVTASFAALADVMIAEPGSRIGFAGPRVIEATMSCTLPEDFQD
                     ASRLVKAGLIDMIVHRHKMRETLAFFLEWFSRD"
     gene            3005..>3241
                     /locus_tag="PLT69_07795"
     CDS             3005..>3241
                     /locus_tag="PLT69_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOJ14376.1"
                     /translation="MVLEGLNPNRWVLGLERVKKALDVLGHPERSYPHVLVAGTNGKG
                     STCMYLERLLGVAGLVVGTNLSPHISRFAERFRVG"
BASE COUNT          602 a         1009 c         1075 g          555 t
ORIGIN      
        1 accggtcggc aagaacacgg cgccgtgcat agggttcgcc gcggtggtca tttcccgcaa
       61 agatccggat gcgaccatgg cggtgttccc ctccgaccac ctcatccgtg agcccgaacg
      121 cctgaaggag gccatcctgt tcggcgtctc cgccctggcc tcgcacccgg acctgctcat
      181 cacgctcggc gtggtgccgg acaggcccga gacaggatat ggctacatag cgcccgagac
      241 cgtgctgctg agccgcggtg gtctcaccat caagagggtg agggccttcc acgagaaacc
      301 cgccaggatc gtggcggaac agtacgtgaa ggacggctac ctgtggaacg cagggatgtt
      361 cctgtggcgg gtggacacca tactggagat gttctcgagg cacatgcccg agctccatga
      421 acagctccag gaactcaagg ccacgatgga cagcgatgcc tctgcggtcg atcgtttctt
      481 caacgaggcg cagtccgtgt ccatagatta cgggatcatg gagaaggccg gacgggtcgc
      541 ggtgatcccc gtggatatag gctggagcga cgtgggcacc tgggacgcca tgggcaacct
      601 ctacgtgacc gacgacaggg ggaattccgt gaggggcccg gccgagctca taaactctga
      661 gaacaacgtg atctggtcga cggacaagcg catcgtgctc atcggcgtga gcgacctcgt
      721 cgtcgtggag gggagcgact cgatcctcgt ctgccccagg gcgttgtcgc aggaggtgag
      781 cgtcgtggcg aagaagctcg gtgatgggaa gtgagtgaca ccggcaggga cgagagacgt
      841 ggccggcctg ccgagtcgtg cccgagtcgg cccccccacg agtgatggaa ccacgtattg
      901 tgcccacggt caggctgtcc gtgttgcctg gcctcccacg cgtgatggaa ccgccggcca
      961 tgggatcagg gcgtcgagcg ccctgcggcc ctgaacccga tggcatggag agggccgctg
     1021 gcgcgatagg cggtgggacg caccgttccg gggatgttct cggactcatc ttccccggtg
     1081 taatgcctgc gatgccgtga aaatcacgca ttgcctctgc gaggaagacc cgacgtcttt
     1141 cgaaaccggg atcctcggtc gttcttcagc ctggcggggg cgcatccagc tcggggcagg
     1201 aagggggagg cgctcgcaga tgccctctga gtacagcagc gacgggttac gggtgggatc
     1261 tcgtcgccgg ggtagagagg tgcccccagt ggacccgtcc gcccgacggc cctggagcgc
     1321 caggcacgcg atctcttgca gccctgcggc ctgcatgcgc ggcatcaaag ccctgccccc
     1381 agggcccgag cccggtcatc ccagcactca ccgtctgcct gcggcctgca tgcgcgtcat
     1441 cgaggcggga tggcctcatg ctccaacgca tgccaacaat ggtatggaaa acgatcaggg
     1501 attcgggtaa agatcacgcc tatgaggata gagatattgg ggtgcagcgg gtcggtcctg
     1561 agcggctttg acaccatgag catcctcgtc gacgggaaga tactcctcga cgccgggtcc
     1621 gtcatgtcaa cgatcggcgt cgatcgcgcg acggggatac gcaccgtcct cctcacgcac
     1681 ccccacatcg accacatcaa ggagctgccc ttcttggtcg atgcgctcta cacccacaag
     1741 gcaaagggca tcaggatcat cgggagccca cagaccatcg acgcattgaa ggcccacata
     1801 ttcaacggtc tcatctggcc cgacatcgcg gagctggacg cgtcgagcga cttcatatcc
     1861 ttggagcacg tgccgggggg cgtcttcgag atcgagggga tggaagtgaa ggcctttccc
     1921 acgaaccaca cgggcggcgc ctacggctac gtgatcaccg agaacgccag gagcgtgctg
     1981 ttcagcggcg acaccgggta tcacccgggc ctcttcgagc tcgtcgggga gctgggatcg
     2041 aaccttgccg cctgtttcat cgaggcgtcg ttccccagca ggatggaaga tctcgcccgg
     2101 atcacccagc acctcacccc ctccctggtc gcgaaggggc tcgacggcgt ggcggcgccg
     2161 gggacgaggg tgatcctcta ccacatcaag ccggcctacc tcgacgaggt gatagcggag
     2221 cttccccgtg ggttcgaata cgtcaggggc ggggaggtgt tcgtcgtttg accgatatcc
     2281 tggagatcgc caagaaggac ggacgatgga ccgagtgccc gaagtgctcg gagatcgtga
     2341 tcaccgagaa actcaggcag aacctctggg tgtgccagca ttgcgggtac cacttccgtg
     2401 tcaacgcccc gacccgcatc gagatgacgt gcgacgaggg ctcgttcgtc gagagcatgc
     2461 ccccggtccg ggaccaggag gagacggccg gctcgcatga cgaggcgata acgtgcggcc
     2521 gcgcccggat catgggccgt gagtgcgcga tcggtgtcat ggatttcttt cacaagggcg
     2581 gctccatggg agcggcggtc ggcgaggcga tcgtgtccct gatgcaggcg gcctcccgca
     2641 agcggctccc gatcgtgatg ttctgcgcgt cggggggcgt acgcgttcag gaaggcatct
     2701 gggggctgtt ccagatgctc aggacagttc atgcccgcac caggacgtcg gacacaccca
     2761 tgatcaccgt gttcaccgac cccaccaccg gcggggtgac tgcgagcttc gcggcgctcg
     2821 ccgacgtcat gatcgcggaa ccgggctcga ggatagggtt cgccgggccg cgcgtgatag
     2881 aagccaccat gagctgcacg ctgcccgagg atttccagga cgcctccagg ctcgtcaagg
     2941 ccggcctcat agacatgatc gtgcaccggc acaagatgcg cgagacgctc gccttcttcc
     3001 tggaatggtt ctcgagggac tgaacccaaa ccgctgggtc ttggggctgg aaagggtcaa
     3061 gaaggccctc gacgtcctcg ggcaccccga acgctcgtac cctcacgtcc tcgttgccgg
     3121 caccaacggg aaaggctcca cctgcatgta cctggagagg ctcctcggag ttgccggtct
     3181 cgtggttggc acgaacctgt cgccccacat ctcgcgtttc gccgagaggt tcagggtggg
     3241 c
//
LOCUS       DAOSCR010000295         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19042_length_3237_cov_3.564111, whole genome shotgun sequence.
ACCESSION   DAOSCR010000295 DAOSCR010000000
VERSION     DAOSCR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19042_length_3237_cov_3.564111"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLT69_07800"
     CDS             complement(<1..655)
                     /locus_tag="PLT69_07800"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ14377.1"
                     /translation="MAKLSHDYIQSLLNNIGELPTLPSIATAIMEKTLDPNANAKQVA
                     DLVEKDQALALKVLKVANSPFYRRIKEISTIRSAVVLLGFNVLKSIVLSISVINLFNK
                     KDKGALDFYSFWQHGIACAVCAKTIASRVIPASAEDAFVAGLLHDLGKVIADQIICPE
                     GGYAEVLETMNTACRDITEVEQEIIGIDHASIGMFLLERWNLPSLLGKAVGYHHSLVD
                     "
     gene            724..1788
                     /gene="ispG"
                     /locus_tag="PLT69_07805"
                     /gene_synonym="gcpE"
     CDS             724..1788
                     /gene="ispG"
                     /locus_tag="PLT69_07805"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOJ14378.1"
                     /translation="MALHREKTRRIQVGACAVGGGAPVSLQSMTNTVTRDVEATVSQI
                     EALARAGCDIVRVSVPDMDSAVAFGEIKRRSMVPLVADIHFDHRLAIESIERGADAVR
                     INPGNIGSRQALRSVAIAARERGVPIRVGVNLGSVKRSVLGRHGDDHVGAIVKSAADY
                     VHMLEDMGLDAIKVSLKSSDVLQTIDAYRRFSKISLWPLHLGVTEAGPLQSGLVRSSV
                     GIGVLLEEGIGDTIRVSLSADPVQEVFAGRVLLECLGLRAEGVRVIACPTCARACADV
                     AAISSELEESLRDVRRHAVVAVMGCVVNGPGEARIADIGVACDRKGALLFRHGKPVRR
                     IRTDEIVTVIREELDKETPA"
     gene            1785..>3237
                     /locus_tag="PLT69_07810"
     CDS             1785..>3237
                     /locus_tag="PLT69_07810"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ14379.1"
                     /translation="MKMTSFFLQTLKEDPRDAEVASHRLMLRSGMIKKLASGIYSYLP
                     LGLKAIRKVERIIREEMDAAGAQECLMPSVQPAEIWKESGRWEVYGKELLRFVDRKGA
                     QFALGPTHEEVITDIVRDAVHSYKDLPLNLYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFDRDEEGAQKSYLAMYDAYERIFARCGLRFKAVEADSGPIGGNFSHEFMVLAETGE
                     DLILSCTSCSYAANQERAEIGGAGGDFPAPPVGAPEKVHTPGIRTVEEVCAFLGVQPS
                     SLIKTLIYSTDIGPVAALVRGDHEISDAKLRRALGCEVVELADEDLIRKVTSAPRGFA
                     GPVGLNVKKVADAALAASGPYVTGANCEDYHVTNVWFGRDVTVDMVADIRVAAGGEAC
                     PRCGKGVLEVIRGIEVGHIFKLGHKYSKAMNALFLDEDGTLKPLVMGCYGIGVGRTVA
                     AAIEQNHDANGIVFPASIAPFHVVVLPITVSDSV"
BASE COUNT          588 a          965 c         1120 g          564 t
ORIGIN      
        1 aatccacgag actgtggtgg taccccacgg ccttgccgag gagagacggc aggttccagc
       61 gctcgaggag aaacatgccg atggaggcgt ggtcgatccc gatgatctcc tgttcgacct
      121 ccgttatgtc cctgcaggcg gtgttcatgg tctcgagcac ctctgcatag ccgccctcag
      181 ggcagatgat ctggtccgct atcaccttgc cgaggtcgtg gagaagcccg gccacgaagg
      241 cgtcctcggc ggacgcgggt atgacgcgcg aggcgatggt cttggcgcat accgcgcagg
      301 cgatgccgtg ctgccagaag gagtagaagt cgagcgcgcc cttgtccttc ttgttgaaca
      361 ggttgatcac gctgatgctg agcacgatgc tcttgagaac gttgaaaccc aggagcacca
      421 cggcgctcct gatcgtggag atctccttga tcctccggta gaagggggag ttcgccacct
      481 tgagcacctt gagggcgagg gcctggtcct tttccaccag gtcggccacc tgcttggcgt
      541 tcgcgttcgg gtcgagcgtc ttctccatga tggcggtggc gatcgagggc agcgtcggca
      601 gttctccgat gttgttcagg agggattgaa tgtagtcatg tgacagcttc gccatctcta
      661 gccttatcga tcgaatctct tccattcttg agtagggttc ccctcctgtg atagtcatgc
      721 ggaatggccc tccacaggga gaaaacgcga aggatccagg tgggtgcgtg tgccgtgggc
      781 gggggggccc cggtgagcct ccagtccatg accaacacgg tgaccaggga tgtcgaggcg
      841 acggtctccc agatcgaggc gctcgccagg gcagggtgcg acatcgtccg cgtgagcgtg
      901 cctgacatgg acagcgccgt tgctttcggg gagatcaaga ggcgctcgat ggtgccccta
      961 gtggccgaca tccacttcga ccaccgcctt gccatcgagt cgatcgagag gggcgcggac
     1021 gcggtgagga tcaaccccgg caacatcggc tcgagacagg cgctgaggtc cgtcgcgatc
     1081 gcggcccgcg agaggggcgt cccaataagg gtcggggtca acctcgggtc ggtgaaacgg
     1141 tccgtgctcg gtcgtcacgg ggacgaccac gtgggcgcga tagtgaagag cgccgcggat
     1201 tacgtgcaca tgctcgagga catggggctc gacgccatca aggtctcgct caagtcgtct
     1261 gacgtgctgc agaccatcga cgcataccgc cgcttctcga aaatctccct ctggccgctc
     1321 catctggggg tgaccgaggc ggggcccctc cagtcgggcc tggtgcgctc gagtgtgggg
     1381 ataggcgtcc tcctggagga gggtattggc gacaccatca gggtctccct ctcggccgac
     1441 cctgtccagg aggtctttgc gggcagggtc ctgctcgagt gcctcggcct gagggccgag
     1501 ggggtcaggg tgatcgcgtg cccgacatgc gccagggcct gcgcggacgt cgccgccatc
     1561 tcctcagagc tcgaggagtc cctcagggat gtgcggaggc acgccgtggt cgctgtcatg
     1621 ggctgcgtgg tgaacggccc cggggaggcg cggatagccg acatcggggt ggcctgcgac
     1681 cggaaggggg cgctgctctt caggcacggc aagccggtgc gacgaataag gactgacgag
     1741 atagtgaccg tcattcgaga agaactcgac aaggagacgc ccgcatgaag atgacctcgt
     1801 tcttcctcca aaccctcaag gaagacccgc gcgacgccga ggtggcgagc cacaggctca
     1861 tgctccgttc cggcatgatc aagaaactgg cttcagggat ctactcgtac ctccccctcg
     1921 ggctgaaggc catcaggaag gtggagagga tcatccgaga ggagatggac gccgccggcg
     1981 cccaggagtg cctcatgccg agcgtgcagc ccgccgagat ctggaaggag agcgggagat
     2041 gggaggtgta cgggaaggag ctcctcaggt tcgtggatcg caagggggcc cagttcgccc
     2101 tcggccccac gcacgaggag gtcatcacgg acatcgtgcg ggacgcggtg cattcctaca
     2161 aggacctgcc cctcaacctc taccagatcc agaccaagtt cagggacgag atcaggcccc
     2221 gtttcggtgt gatgagggcg agggaattca tcatgaagga cgcctactcc ttcgaccgcg
     2281 acgaagaggg ggcccagaag agctacctgg ccatgtacga cgcctacgag aggatcttcg
     2341 ccaggtgcgg tctcaggttc aaggccgtcg aggccgactc cgggcccata ggcgggaact
     2401 tctcccacga gttcatggtg ctggccgaga ccggggagga cctcatcctc agctgcacgt
     2461 cgtgctcgta cgcggccaac caggagcgtg cggagatcgg cggtgcgggc ggcgactttc
     2521 cggccccgcc cgtgggagcc cccgagaagg tgcacactcc cggcatcagg accgtggaag
     2581 aggtgtgcgc cttcctgggg gttcagcctt ccagcctcat caagacgctg atctactcca
     2641 cggatatcgg cccggtcgcg gccctggtga ggggcgacca cgagatcagc gacgccaagc
     2701 tcaggagggc gctgggttgc gaggtggtgg agctcgccga cgaggacctc atccggaagg
     2761 tcacctccgc gccgaggggt ttcgccggtc ccgtcggcct gaacgtgaaa aaggtggccg
     2821 atgcggccct cgcggcctcc ggcccgtacg tgacgggcgc gaattgcgag gactaccacg
     2881 tgacgaacgt gtggttcgga agggacgtca ctgtcgacat ggtcgcggac atacgggtcg
     2941 ccgccggggg tgaagcgtgc ccgcgctgtg ggaaaggggt cctcgaggtg atccggggga
     3001 tcgaggtcgg gcacatcttc aagctcggac acaagtactc gaaggccatg aacgcgctct
     3061 tcctcgacga ggacgggaca ctcaagcccc tggtcatggg gtgttacggg ataggggtcg
     3121 gcaggacggt cgcggccgcc atagagcaga accacgacgc gaacggcatc gtgttcccgg
     3181 cctccatcgc gccgttccat gtcgtggtgc tcccgatcac cgtgagcgac tcggtga
//
LOCUS       DAOSCR010000296         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19046_length_3236_cov_3.433826, whole genome shotgun sequence.
ACCESSION   DAOSCR010000296 DAOSCR010000000
VERSION     DAOSCR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19046_length_3236_cov_3.433826"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLT69_07815"
     CDS             <1..565
                     /locus_tag="PLT69_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ14380.1"
                     /translation="FECPEQNGMHIWEDAFLVEVVDPVTLEPVAPGMQGELVITTLER
                     EAMPILRYRTKDLTTLSDEPCPCGRTHRRISRIAGRSDDMIILKGVNIFPMQIEAVLI
                     GIPGVGRNYQVILESRGHNDHITVKVEIDAHLSIDDYRSLEELRKRIVRELRDEILVT
                     PEVVLTEPGGIPVAQGKAVRVVDNRTT"
     gene            complement(774..977)
                     /gene="tatA"
                     /locus_tag="PLT69_07820"
     CDS             complement(774..977)
                     /gene="tatA"
                     /locus_tag="PLT69_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOJ14381.1"
                     /translation="MFGGLGMTELIIILLIVLVIFGAGKLPEVGSALGKGIKNFKSAI
                     SDSGDKTPKADLEDKTKEGQPKG"
     gene            complement(1126..1455)
                     /locus_tag="PLT69_07825"
     CDS             complement(1126..1455)
                     /locus_tag="PLT69_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOJ14382.1"
                     /translation="MDDILLSALRANEAVAANASSNIANMNTPSYRALKTTIVSSSPE
                     ALDVVTLTSDERGPLDEEGNEMSNVDPAREIVVLMQAKNAFEMALKAISTREEMLNDL
                     VESLAER"
     gene            1489..2970
                     /locus_tag="PLT69_07830"
     CDS             1489..2970
                     /locus_tag="PLT69_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ14383.1"
                     /translation="MSIILTVKRLGLLRKMVLAYIVFVVVLSGLTLVLFVVSTRVSGL
                     AGRIYLVDYRKKEITDRLIANLIAVEETGKQYIILRNDAYRTILDQQYDDITRAWDIL
                     LSGGMTRDETERNVAENGRGLWMAFWVRFNSQLKELPPDQAALDELFARNSSEIDQVV
                     SAARYINRNAVDSLRSNIASLKDLGDEITAWTWWALVLGLSIGLVVPVLLSVSVVRDL
                     NRIKEGIAHISEGDFSYRIDLASTDELGMLAESFNKMASRLKELDEMKSDFISMVSHE
                     LKTPLTSMKEAASLLMEGHAGGVSDKQKRLLEIMDQGIRRLLNTVSELLDMSRIASGT
                     IALDKGVWDMNETVAAFVSEIWPIADANGVRIKAVYAEGGCRVLVDRNKILQVLTNLT
                     HNAIKSSPEGSEVEIRVRNTGGCVVTEIEDHGKGIPEEDLPRIFEKFFQSRSTRGHGG
                     MGLGLAISKGIVDAHGGTIYAQSKVGKGSVFAFSLPCADTGLP"
     gene            2996..>3236
                     /locus_tag="PLT69_07835"
     CDS             2996..>3236
                     /locus_tag="PLT69_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14384.1"
                     /translation="MSIGEAEASLSQGKAPKQDLRELEKQATADVLARIRIAMIVESA
                     VKGEVNYASAKAELKDIRDKAELPSYLRVEAGYLLV"
BASE COUNT          640 a          942 c         1059 g          595 t
ORIGIN      
        1 cttcgaatgc cccgagcaga acgggatgca catctgggag gacgccttcc tcgtggaggt
       61 ggtcgacccg gtcacgctcg agcccgtggc cccgggtatg cagggggaac tggtcatcac
      121 gaccctcgag cgggaggcca tgccgatcct caggtaccgc accaaggacc tgacgacgct
      181 gtcggacgaa ccgtgcccct gcggcaggac acaccgccgc atctcgcgga tagcaggccg
      241 cagcgacgac atgatcatcc tcaagggcgt gaacatcttc cccatgcaga tcgaggcggt
      301 tctcataggc attccaggcg tgggaagaaa ctaccaggtc atcctcgagt ccagggggca
      361 caacgaccac atcaccgtca aggtcgagat cgacgcgcac ctctccatcg acgactaccg
      421 gtcgcttgag gaactgagga agcggatcgt gagggagctc agggacgaga tcctggtcac
      481 ccccgaggtc gttctcacgg aaccgggagg gatacccgtg gcccagggca aggcggtccg
      541 ggtcgtggac aaccggacga cctaggcgtc tttgagaggc accggtgcgg tcgggcggcc
      601 agggttccct cgcctgccgg agggccgcac ggccctgtac acgcagactt tcaggcaacg
      661 ttgccttggt ctccggcaat gatgcttggg cggccctgtg cgcgcaggct ttcatcctgc
      721 acaatgggct ttcgtgcgag gtttcctgag aaagcgggcc tcgtcctctc ggttcagccc
      781 ttgggttgcc cctctttggt cttgtcctcg agatccgcct tgggtgtctt gtcgcccgag
      841 tcgctgattg cggacttgaa gttcttgatg ccctttccca gagcgctgcc cacttcggga
      901 agcttgcccg caccgaagat cacgagaacg atcaagagga tgatgatcag ttccgtcatg
      961 ccgagaccgc cgaacatggg tcagccctcc ttgcctttgg ctgattgagc cttaccacat
     1021 cgcgcaggac atggcaacgt gcgcgtgatc ggcccgtgtg gtggggggca acggtggtcg
     1081 caacaccaca caaggcggcg ctggcgatcg gaccgcgcgg gggagtcaac gctccgccag
     1141 ggactccacg agatcgttca gcatctcctc tcgtgtcgag atggctttca gcgccatctc
     1201 gaaggcgttc ttcgcctgca tgaggaccac gatctcccgg gcaggatcca cgttcgacat
     1261 ctcgttgcct tcctcgtcga gcggcccccg ttcgtccgag gtcagggtga ccacgtcgag
     1321 ggcttccggc gaggacgaga cgatggtcgt cttgagggcc ctgtaggagg gcgtgttcat
     1381 gttggctatg ttcgaggagg cgttggctgc cacggcctcg ttcgcacgca gggctgaaag
     1441 gaggatgtcg tccatggtcg atgtcttcct tgaagatgta tcggcaaggt gtctataatc
     1501 ttgaccgtga agaggctcgg tctgctccgg aagatggtgc tcgcctatat cgtgttcgtg
     1561 gtcgttctct cgggcttgac cctcgtcctc ttcgtggtct ccacccgggt gagcgggctc
     1621 gccggcagga tctatcttgt ggattaccgc aagaaggaga tcacggacag gctgatcgca
     1681 aacctcatcg cggtcgagga gacggggaag cagtacatca tcctgaggaa cgacgcgtac
     1741 aggaccatcc tcgaccagca gtacgacgac ataacccggg cttgggacat cctgctgtca
     1801 gggggaatga cgcgcgacga gacggaaagg aacgtcgccg agaacggccg ggggctgtgg
     1861 atggccttct gggtgaggtt caactcccag ctcaaggaac tgcccccgga ccaggctgcg
     1921 ctcgacgaac tgttcgcgag gaacagctcc gagatcgacc aggtggtgtc ggcggcgagg
     1981 tatatcaaca ggaacgccgt ggactcgctg aggtcgaaca tagcctctct caaagacctg
     2041 ggcgacgaga tcacggcatg gacgtggtgg gcgctcgtcc tcgggctgtc catcgggctc
     2101 gtcgtccccg tgctcctgtc ggtgagcgtc gtgagggacc tcaacaggat caaggagggg
     2161 atcgcccaca tctcggaggg ggacttttcc taccgcatag acctcgcgag cacggacgag
     2221 ctcgggatgc tcgccgagtc gttcaacaag atggcgtcca ggctcaagga actcgacgag
     2281 atgaagtcgg acttcatatc catggtctcg cacgagctca agacgcccct cacgtccatg
     2341 aaggaggccg cgagcctgct catggaggga cacgcagggg gcgtctccga caagcagaaa
     2401 cggctcctgg agatcatgga ccagggcatc aggaggctcc tgaacaccgt gagcgagctc
     2461 ctggacatgt cgcgcatcgc cagcggcacc atcgcgctcg acaagggggt ctgggacatg
     2521 aacgagacgg tggcggcgtt cgtctcggaa atatggccca tcgccgatgc caacggggtg
     2581 aggataaagg cggtctacgc cgagggcgga tgcagggtgc tcgtcgacag gaacaagatc
     2641 ctgcaggtct tgacaaacct cacgcataat gccatcaaga gctcacctga aggttccgag
     2701 gtggagatca gggtcaggaa caccggaggg tgtgtcgtga ccgagatcga agaccacggg
     2761 aaagggatac ccgaagaaga tctgccccgg atttttgaga agtttttcca atcccgatcg
     2821 accagaggcc acgggggaat gggcctgggc ctcgcgatct cgaaggggat cgtcgacgcg
     2881 cacgggggaa ccatatatgc gcagagcaaa gtgggcaagg ggagtgtctt cgctttcagt
     2941 cttccttgcg ctgatactgg ccttccttga ggggtgctcg gctctccagt cctacatgag
     3001 catcggcgag gcggaggcct cgctctctca aggcaaggcg ccgaagcaag acttgaggga
     3061 gctcgagaag caggcgactg ccgacgtcct ggccaggatc cgcatcgcca tgatcgtgga
     3121 gtccgccgtg aagggcgagg tgaactatgc gtcggcgaag gccgagctga aagacatcag
     3181 ggacaaggcc gagctgccct cgtacctcag ggtcgaggct ggctacctcc tggttt
//
LOCUS       DAOSCR010000297         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19056_length_3236_cov_2.352091, whole genome shotgun sequence.
ACCESSION   DAOSCR010000297 DAOSCR010000000
VERSION     DAOSCR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19056_length_3236_cov_2.352091"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1334)
                     /locus_tag="PLT69_07840"
                     /pseudo
     CDS             complement(30..1334)
                     /locus_tag="PLT69_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
     gene            complement(1331..2989)
                     /locus_tag="PLT69_07845"
     CDS             complement(1331..2989)
                     /locus_tag="PLT69_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOJ14385.1"
                     /translation="MILGIDVGGTHTDAVCIEDGRIRSYAKTLTGDDLVASVVDAIER
                     LDAACPKVKRVVLSTTLSTNVIVEGKHPPTGVVCSAGPGIDPGTFSVGTCFRHVAGAM
                     DHRGREYIPIDPAEVMDAASTLERAGCQGVAIVSKFSVRNPAHEDAIASLVGSRFRHV
                     CLGHTLSGALNFPRRINTTYLNAAVMPVQERFISAVASAVERLGIQAPLYFLKADGGT
                     YTQDAARRFPVETILSGPAASVMGALALAPSRDASVLVIDIGGTTTDIGVLVGGLPLL
                     DTWGVAIGGLKTLVRGLKVISIGVGGDSCISVEDDGTIAVGPRRDGMPACMGGPSATP
                     MDALVILGEYAGDTARALDAVRALALKAGMDIEPFCRKVIGVMVDAVHGAAKAFMEEI
                     NAHPVYTIYEMLHPHVVKPEGVVLVGGPAALLAEYVSRSFGLPCHVPEHAGVANALGA
                     ALSRTTAQITLHADTELGVLFCPELGFKAAAPRSMTLEDLRHEGLRLLRENASYLGLE
                     DSAEAQVIQQQAFTMVRGFSTVGANMRVRVQNTPGIIDSWGGLG"
BASE COUNT          540 a         1049 c         1066 g          581 t
ORIGIN      
        1 gcggctcaag agcggcatgc cttccctcgt cacatgatct tcacggtaaa ctcgtccgcg
       61 tcgagatcct ggaggtacac ggcataatac ggcccgcccg ggtgatcgaa ccactcgcgt
      121 gcctggctgc ggcacgcgct gcacgcaccc ctgggcaggt gcacggcgag gatcctgtag
      181 cccgtggagg cgcgcgagtc tatcctgagc gcgccgttac acgccgtgca ataagcaagg
      241 gtctcgtgct gtcgctcggt gatggaatcg gtgtcgtaga agatgctcct cgacctctcg
      301 tagaagggcc tcggctccgg cgcgagggcc gagcggttcc gccacatgtc atagatctgc
      361 tcgcagagac gctgtatgta ctcggtcacc ctcgggtcct gcctgacgcg ctcggggtcg
      421 tagtccggct ccctgacgta cgagtagtcc aggcccgcca gcgccaggat gatgcccgtg
      481 ttcacgtacg gcagggcccc ctcgatggag taccctccct cgagcacgca cacgtgcggc
      541 ttgaggagct cgttgaggcg ggcgtagccg agggcggaaa acgacatgtt cgtgatgggg
      601 tccgtgaagt ggttgtcttg gccggccgag tttatcacga ggtcgggccc gaagtcttcc
      661 agcacgggga gcaccacgtg ctcgacggcc cagaggatgc cctcgtcgcc ggtcaaaggc
      721 ggcaggggga tgttgatggt gtagcctgcg gccttggggc ctccgaactc ggacgggaat
      781 cccgagccag ggtagagggt cctgccgtcc tggtggatgg agatgtagag aacgtcgggg
      841 tcgtgccagt atatgtcctg ggttccgtcg ccgtggtggc agtcggtgtc cacgaccgcg
      901 atcttcttca cgccggggta atgggtgcga actcactcca gcatcaccgc ctcgatgttg
      961 atgttgcaga acccgcgcgc accctgcacc acccgcatgg catgatgccc gggcggcctc
     1021 accagtgcga acgcgttgtc gaccaccccg tcgagcacga gccgtgcggc ttcgatgcat
     1081 ccgcccgccg agatgttgtg ggaagccgtg atcctgctcg gcacgtcagg cacgcagaag
     1141 tgcaccctcg agatgtcttg ctccgaggcc acgccggcct tgaactcgta gacgccctcg
     1201 atatcgagaa cgccttcctc gaggacctgg tcctgcgtgt agagaaggcg ctccttccgc
     1261 tcagggtggg tgggagatat ctcccagtcg tacgcaggga aaaagatgag ccctgtgcgc
     1321 ctcttcgcct tcatccgagg ccaccccacg aatctatgat acccggcgtg ttctgcaccc
     1381 tgaccctcat gttcgccccc acggtggaga accccctgac catggtgaat gcctgctgct
     1441 ggatgacctg ggcctcggcc gagtcttcga ggccgaggta cgaggcgttc tccctcagga
     1501 ggcgcagtcc ctcgtgcctg agatcctcca gggtcatgga cctcggagcc gcggccttga
     1561 acccgagctc gggacagaac aggacgccga gctcggtatc cgcgtggagc gttatctgcg
     1621 cggtggtccg cgagagcgcg gcgcccaggg cgttggcgac gccggcgtgc tcgggcacgt
     1681 ggcacggcag gccgaacgac ctggagacgt attcagcgag caacgcggca gggccgccga
     1741 ccaggacgac cccttcgggt ttcaccacgt gcgggtggag catctcgtag atcgtgtaca
     1801 cgggatgggc gttgatctcc tccatgaacg cctttgcggc gccgtgcacg gcatcgacca
     1861 tcacgccgat caccttccgg cagaagggtt cgatgtccat ccctgccttg agcgcgagcg
     1921 cccggaccgc atcgagcgca cgagcggtat cccccgcgta ctcgccgagt atcacgaggg
     1981 cgtccatggg ggtggcggag ggtccgccca tgcacgcggg catcccgtcc ctcctcgggc
     2041 cgacggcgat cgtgccgtcg tcctcgaccg atatgcacga gtcaccgccg acgcctatcg
     2101 agatcacctt gagccccctg acgagcgtct tgagaccccc gatggccacg ccccaggtat
     2161 ccaggagggg taggccgcct acgaggacac ctatgtccgt ggtcgtcccg cctatgtcga
     2221 tgaccaggac ggaggcgtct ctggagggtg cgagcgcgag agcgcccatg acgctcgctg
     2281 caggcccgga aaggatcgtc tcgacgggga accgcctcgc cgcgtcctgg gtgtaggtgc
     2341 cgccgtccgc cttgaggaag tacagcgggg cttgtatgcc cagcctctcg accgccgagg
     2401 ccacggcgga gatgaaccgt tcctgtacgg gcatcaccgc ggcgttgagg taggtcgtgt
     2461 tgatgcgtct cgggaagttg agcgcgcccg acagcgtatg gccgaggcag acgtgcctga
     2521 agcggctgcc gaccagggat gctatcgcat cctcgtgcgc cgggttcctc acggagaact
     2581 tggaaacgat cgccacaccc tggcaccccg cacgctcgag ggtggaggcg gcatccatca
     2641 cctcggcggg atcgatgggg atgtactccc tgccccggtg atccatggcc cctgccacgt
     2701 ggcggaagca tgtccccacg gagaacgtcc cgggatcgat gcccggaccg gccgagcaga
     2761 ccacgcccgt gggcgggtgc tttccctcca cgatgacgtt cgtcgacagg gtggtcgaga
     2821 gcaccaccct cttgaccttc gggcaggccg catccagcct ctctatggcg tctaccaccg
     2881 aggcgacgag gtcgtcaccc gtgagcgtct ttgcgtagga tcggatcctg ccgtcctcta
     2941 tgcagacggc atcggtgtgg gttcctccta catcgattcc caggatcacg ctcggctccc
     3001 cgccgcgcct cgggccgcac ggaatcggcg gacacccgaa agggcggcgc atcgtgtcgc
     3061 tcttcgcgat cataacaggt cgaaagctcg acttcaacag gcttcggaca aacggggccc
     3121 ctgcgcttgt tgcgcgacgg gcatcgatct ggtaaatctc gcagggacga agactcggag
     3181 gtggacgtga tcaagagact gtgtgcgctt gttgtcctgc tgtccgtcct cgggtg
//
LOCUS       DAOSCR010000298         3232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19077_length_3232_cov_3.053510, whole genome shotgun sequence.
ACCESSION   DAOSCR010000298 DAOSCR010000000
VERSION     DAOSCR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19077_length_3232_cov_3.053510"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1116
                     /gene="mtnA"
                     /locus_tag="PLT69_07850"
     CDS             142..1116
                     /gene="mtnA"
                     /locus_tag="PLT69_07850"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOJ14386.1"
                     /translation="MITPFEFVRGAFRILDQRLLPSRVEWIECTCASHVAQAIRTLAV
                     RGAPAIGIAAAFGLALEAKSGRERLEAAADLLVKARPTAVNLAWAVRRCMERAALTED
                     GLLAEALEAEAFAIWDEEREANEAMAELGSLLFDPGRRHRVLTHCNAGALATGGIGTA
                     LGVVKRLNEKGLLERVYVDETRPLLQGARITAFELVTDAVPCTLVTDSTAGWLMRRRV
                     VDAVIVGADRIALNLDTANKIGSYSLAVLAHAHGVPFYVAAPISTFDRATPSGEGIPI
                     EERAPEEVLEFAGAKVAPGVDVYNPAFDVVPAGLVSAFITEKGIVRRV"
     gene            1699..>3232
                     /locus_tag="PLT69_07855"
     CDS             1699..>3232
                     /locus_tag="PLT69_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14387.1"
                     /translation="MSGIYETIVENANEFIVVIQNGRIRFLNRKARGLPCFACDSGQG
                     AAFEEYIHPDDRPLVLERLERRLSGDEVENVSVFRIVDPSGRLYWVEDNAVLIEWEGR
                     PAVLNFITDITEQKRMETQLKESEERARLYLEHASDLIFVVDRQGRITTISPRVEGVI
                     GYCARELLNKNVRELDILADGYIRKAIEQVKRVLDGQRIQSSLYEVIAKDGVSRYVEV
                     SMAPLTLGEERSHVICVARDVTDRKSMEETLKRIETRYREFLEEIEDGYFEVNLNGDF
                     TFFNSVMCRILGYPQEMMLGMNYRRFMDEATAEKVFSAFNQVYRTGEPAKGFEWSLIK
                     NDGTACHVETTVALMRGEDGMPSGFRGTVRDVTEQKRLEAHLHQAQKMEALGTLAGGI
                     AHDFNNILSGILGYAELALMDSGADEWVSHRIGQIVKACQRAKSLVNQILSFSRRGEA
                     TIRPVILKNEVEEALKLIRASIPSNIEIRTALGAAEEIVHADPARIHQIVVNLCTNAY
                     QAM"
BASE COUNT          684 a          975 c         1040 g          533 t
ORIGIN      
        1 gaccgtcccc cttctcgaga aggtcatgga cgatggggtg atcgtcaagc agccgccgac
       61 ggtacgccag atgcgggccc tggccgtgga atccgtggac aggctcaagg gcacgacccc
      121 cgagcgggag gtgctcggcc tatgatcacg cctttcgagt ttgtgcgggg cgcgttcagg
      181 atcctggacc aaaggctctt gccctcgagg gtggagtgga tcgagtgtac gtgcgcctcc
      241 cacgtcgcgc aggccatccg gacgcttgcc gtcaggggtg ccccggcgat agggatagcc
      301 gcggccttcg gtctcgcgct cgaggcgaag tcggggaggg agcgcctcga ggccgctgcg
      361 gatctcctcg tgaaggcgag gcccacggcg gtgaacctgg cctgggcggt caggaggtgc
      421 atggagaggg cggccctgac ggaagacggc ctcctcgccg aggccttgga ggccgaggcg
      481 ttcgccatct gggacgagga gagggaggcc aacgaggcca tggctgaact cgggtcgctg
      541 ctgttcgatc ccggccgcag gcaccgggtg ctcacccact gcaacgcggg cgccctcgcg
      601 acagggggta tcggaaccgc gctcggggtc gtcaagaggc tgaacgaaaa gggcctgctc
      661 gagagggtgt atgtcgacga gacgaggccc ctgctccagg gcgcacgcat aacggcgttc
      721 gagctcgtaa cggacgccgt accatgcacc ctcgtcacgg actccaccgc cggctggctc
      781 atgcgcaggc gtgtcgtgga cgccgtgatc gtaggtgcgg acaggatcgc cctgaacctg
      841 gacaccgcga acaagatcgg cagctattcg ctcgccgtgc tcgcgcacgc ccacggggtg
      901 ccgttctacg tggccgcccc catctccacc ttcgacaggg cgacgccgag cggtgagggg
      961 atacccatcg aggagagggc gccagaggag gtgctcgagt tcgcgggggc gaaggtggcc
     1021 cctggggtgg acgtctacaa cccggccttc gacgtggtgc ccgccggact cgtctccgcc
     1081 ttcatcacag agaagggcat cgtgaggcgt gtctgagaaa tacgtcatgg ggaatcgccc
     1141 gggtgccgag gaacgccggt aaagcacgag gtcgatgctt caggcccagg tgaatcgccc
     1201 gtgtacgccg aggaacgccc ttgagacggg tggcgccgtt tccccgccgc gcgtgcccct
     1261 ccctcgcgca ccccgcgccc tggatgggct tttccctgcc ggaacgacgg atgcccgccc
     1321 cccataacgg tccgcccgtc atcgcgcatg aacgctttcc cgctcaccct ccgatgttcc
     1381 ctgtccagaa ccgtctggtt tcatgggact ttccttcaca ccagggaccc atcctgctga
     1441 cacaatgcag cacttaaaac aatataaaaa agggcataaa gggtaacgga ataccctccc
     1501 gccctggaag cagtcaccgg gggatgcgac gaaaaccgac cctgttcatt tcatagcaaa
     1561 gttaaataaa ataaatattg cttgacttgt gcactgaaac cagatacagg acgaggtcca
     1621 cctacacgac gcccagccag gaggacgcgc tcctcgaaaa gctataataa aaacaccacc
     1681 cacgagacct tgcacaccat gagcggcatc tacgagacca ttgtcgaaaa cgcaaacgag
     1741 ttcatcgtcg tcatccagaa cggccgtatc cgtttcttga accggaaggc ccgcggactc
     1801 ccgtgctttg cctgcgacag cgggcaaggt gcggcgttcg aggagtacat tcacccggat
     1861 gacaggcccc ttgtcctcga acggctcgaa aggaggcttt caggggacga ggtcgagaac
     1921 gtgtccgtct tcaggatcgt cgatccttcc gggagactct actgggtcga ggacaacgcg
     1981 gttctcatcg agtgggaggg gaggccagcc gtcctcaact tcatcacgga catcacggaa
     2041 cagaaacgca tggaaacaca gctgaaggag agcgaggaac gcgcgaggct ctacctcgag
     2101 cacgcatccg acctcatctt cgtggtggac cggcagggac gcatcaccac catctccccg
     2161 agggttgaag gcgtgatcgg ctactgcgcg agggagctct tgaacaaaaa cgtgcgcgag
     2221 ctcgacatct tggctgacgg atatatccgg aaggccatcg agcaggtcaa acgcgttctc
     2281 gacgggcaga ggatccagtc ctcgctgtac gaggtcattg cgaaggacgg ggtctctcgg
     2341 tacgtggagg tcagcatggc ccccctgaca cttggcgaag aacgttccca tgtgatctgc
     2401 gtggccaggg acgtgaccga ccggaagagc atggaagaga ccctcaagag gatcgagacg
     2461 aggtaccgcg agttcctgga ggagatcgag gacgggtact tcgaggtgaa cctgaatggc
     2521 gatttcacct tcttcaacag cgtcatgtgc aggatcctgg gatacccgca ggaaatgatg
     2581 ctcgggatga actacaggcg cttcatggac gaggcaaccg cggaaaaggt cttctccgcc
     2641 ttcaaccagg tctacaggac gggggagcct gccaagggct tcgagtggtc tctcataaaa
     2701 aacgacggca ccgcgtgcca tgtcgagacc acggtcgcgc tcatgagggg ggaggacggc
     2761 atgccgtccg ggttcagggg aactgtgagg gacgtcaccg aacagaaacg cctggaggcg
     2821 cacctccacc aggcacagaa gatggaggcg ctcgggacgc tcgcaggggg tatcgcccac
     2881 gacttcaaca acatcctctc cgggatcctg ggctatgcgg aactcgcact catggactca
     2941 ggcgccgatg agtgggtgtc tcaccgtatc ggccaaatcg tgaaggcgtg ccagagggcg
     3001 aagtcactgg tgaaccagat cctctcgttc agcaggagag gggaggcgac catacgaccc
     3061 gtcatcctga aaaacgaggt ggaggaggcg ctgaagctga tcagggcgtc cataccctcg
     3121 aacatcgaaa tacggaccgc cctgggcgcg gccgaggaga tcgtgcatgc cgaccctgcc
     3181 cggatacacc agatcgtcgt gaacctctgc accaatgcct accaggccat gg
//
LOCUS       DAOSCR010000299         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19119_length_3227_cov_3.883670, whole genome shotgun sequence.
ACCESSION   DAOSCR010000299 DAOSCR010000000
VERSION     DAOSCR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19119_length_3227_cov_3.883670"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..1421)
                     /locus_tag="PLT69_07860"
     CDS             complement(345..1421)
                     /locus_tag="PLT69_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ14388.1"
                     /translation="MGDIDKKTLYENLARHLHQGVIGAPLSPSLLKILEIMFTEEEAE
                     VALRLPFENKTLGEIERLIPDKAPRLENILSAMAKNGTVFTLQKPGKKRVYRLLPTVV
                     GFAEAPFWPGVPSEKARKLAPLWIQYRNEGFGEELSRNIPAVRVIPIDRSLRNSSEIL
                     PFDRIREKMKTVKYFAVSKCPCRQMMAYEGKGCSHSLENCLHFNDMGRYVVEQGMGRE
                     ISMEEAVRILEEADGEGLVHTVENLDGFMSMICNCCGCCCVFLQTQKKMNLRMISSSN
                     YVSKVDSESCAGCGTCCRRCPMDAVAVDDSGTAKVDAERCIGCGVCAPTCPTGAIALV
                     LRQDVSAPPDVGEFLAKRYLAPAG"
     gene            complement(1941..2735)
                     /locus_tag="PLT69_07865"
     CDS             complement(1941..2735)
                     /locus_tag="PLT69_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ14389.1"
                     /translation="MNTDAKERIKEFALSLGVEDVGIGSVRDYKSPRSPSIESIFAGA
                     RSMVVLAFSELSNYLSTNKRIAMAGRIDSLAFARACVYRITRFIEKEFGALSMSTPFS
                     YPLNMSPESKFGLIADFSQRHAAVAAGLGTIGRHNLVIHPRLGTRVVFSTVLTELELE
                     PDSPVTEDLCTHCNLCVEACPAHALDEEGKTHEFKCLSCSQPNGLASSIAFWLRFVDS
                     PPEVQKRMIASPEYMSLYQAQSLVLEYHCFACYLACPVGVKKGASA"
     gene            complement(2732..>3227)
                     /locus_tag="PLT69_07870"
     CDS             complement(2732..>3227)
                     /locus_tag="PLT69_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ14390.1"
                     /translation="VNFAMNDKCAAGTGRFLEAMARVLQVDIDEMGELDRGARGDVTI
                     SSICTVFAESEVVSLIARGENICEIALGLNRSIASRTVSLVKRVAPDIDGMGIAMSGG
                     VARNRGVVRALEEALGRPIFVPEAPDLVGALGAALFALERGARRVSRDDGRHADGRRE
                     GECA"
BASE COUNT          493 a         1109 c          994 g          631 t
ORIGIN      
        1 ctcctccggg gtgtagagct ccctcaagat cccgtggagg gtttcgttcc atggcgccct
       61 caccacgatc ccgtcgatct tgcggccgag ctccctgtaa acggttttct ctccttcccg
      121 gtccatgacc cctcctttgt ccgtgcacgg agacctggtg cgccgccctg ccgtttcaac
      181 ggcgcctgtt gatggactct ccacgcaggg ccgtgtggaa gctcgccaac gtaatggata
      241 atgcggccag caagggcatt gtcaactccc gggcatctgc cctccgtccc ggtgaagcga
      301 gccgtccgaa cccccccgag ccatgcaggg cgctcggacg agcctcaccc tgccggcgcg
      361 aggtagcgtt tggccaggaa ctcgcccacg tccggcggcg ccgacacgtc ctgcctgagg
      421 accagggcga tggcccccgt ggggcaggtc ggggcgcaga cgccgcaccc tatgcaccgt
      481 tcggcgtcca ctttcgcggt gcccgaatcg tccacggcca ccgcgtccat ggggcacctt
      541 cggcagcatg tcccacagcc tgcgcacgac tcgctgtcca ccttcgatac gtagttcgac
      601 gaggatatca tcctcaggtt catcttcttc tgcgtctgga ggaacacgca acagcacccg
      661 cagcagttgc agatcatgga catgaagccg tccaggtttt ccacggtgtg cacgaggcct
      721 tccccgtcgg cctcctcgag gatcctgacg gcctcttcca tggagatctc cctgcccatg
      781 ccctgttcca ccacgtagcg gcccatgtcg ttgaagtgca ggcagttctc cagcgagtgg
      841 ctgcagccct tgccctcgta ggccatcatc tgcctgcatg ggcacttgct caccgcgaag
      901 tatttgaccg ttttcatctt ctcgcgtatg cggtcgaagg ggaggatctc ggagctgttc
      961 ctcagcgagc ggtcgatggg tatgaccctc accgcgggga tgttccgcga cagctcctct
     1021 ccgaagccct cgttccggta ctggatccac agcggggcca gcttcctggc cttctccgag
     1081 gggacacccg gccagaaggg cgcctcggca aagcccacca ccgtcggcag gaggcggtag
     1141 acccttttct tcccgggttt ctgcagggta aagaccgtgc cgtttttcgc catggccgag
     1201 aggatgttct cgagcctggg cgccttatcg gggatcagcc gctcgatctc gcccagggtc
     1261 ttgttctcga agggcagcct taaggccacc tccgcctcct cctcggtgaa catgatctcg
     1321 agaatcttca agagcgacgg cgacaagggt gcgccgatca cgccctggtg caggtgtctt
     1381 gccaggtttt cgtagagcgt ctttttgtcg atgtccccca tgcgtttctc cttccattgc
     1441 tgacgatcta tgcacctcgt ccggtgtcgc ccgccgtcga ccgtggtgtc tgacggtttg
     1501 ccccggaggt tcccccgtat aacccctcgt cctggagtgc cgacgtccac cctggtccca
     1561 gcctgcaggt cgaccgttct ttcttccgcc ccgccttctt gtatgaacgc ctggccaggg
     1621 ggactgccgc ggccctgtgc aggcaggacg accgtccagc ccctaggcgg ccttcgatgc
     1681 atgcggcaat ccctgggcgt cgccagtgca tgcacacacc ctgtctttca ggccggggcg
     1741 agcgttcctg catgaatgcc gggccggctg cgagtgctcc cgccaccggg ggccgccggg
     1801 ggatcctcgt tcccttccgc tgcggccctc ttgcccgtca cggtgcgtcc cgggtctgca
     1861 agggccgctc ccgacatgct gccatcgcgc ccgcctgggc tcttcccggt gcgacggggg
     1921 cgttgacctg ccatggcgcc tcacgcggat gcgcccttct tgacccccac cgggcaggcc
     1981 aggtagcacg cgaagcagtg atactcgagc acgagggact gggcctggta caggctcatg
     2041 tactcggggc tggctatcat ccgcttctgc acctcgggag ggctgtcgac gaacctgagc
     2101 cagaacgcga tgctcgatgc cagcccgttg ggctgggaac agctcaggca cttgaactcg
     2161 tgcgtcttgc cctcctcgtc gagggcgtgg gccgggcagg cctcgacaca gaggttgcag
     2221 tgcgtgcaca ggtcttcggt cacgggactg tccggttcga gctcgagctc ggtgagcacg
     2281 gtggagaaga cgaccctcgt gccgagccgg gggtggatca cgaggttgtg cctgcctatg
     2341 gtccccaggc cggcggcgac cgcggcgtga cgctgggaga agtccgcgat gaggccgaat
     2401 ttgctctcgg ggctcatgtt caggggatac gagaagggcg tcgacatgga cagggccccg
     2461 aactccttct cgatgaaccg ggtgatgcgg tacacgcacg ccctggcgaa cgcgagggaa
     2521 tcgatcctgc ccgccatggc gatcctcttg tttgtgctga ggtagttgga aagctcggag
     2581 aacgcgagga ccaccatcga tcgcgccccc gcgaagatgg actctatgga aggcgacctg
     2641 gggctcttgt agtccctgac cgagccgatc ccaacgtcct ccacgcccag gctgagggcg
     2701 aactccttga ttctttcctt cgcgtctgtg ttcatgcaca ttctccttcc cggcgaccgt
     2761 cggcatgcct gccgtcatcc ctcgaaaccc gacgggcccc cctctcgagg gcgaagaggg
     2821 cggcgcccag ggcgcccacg aggtccggcg cctcggggac gaagatcggg cgacccagcg
     2881 cctcctcgag ggccctgacg acgcccctgt tcctcgcgac gccacccgac atggcgatgc
     2941 ccatgccgtc gatgtcggga gcgacgcgct tgacgagcga gaccgtccgc gacgcgatgg
     3001 agcggttcaa ccccaacgct atctcgcaga tgttctctcc ccgggcgatg agcgagacca
     3061 cctcgctctc ggcgaacacc gtgcagatgc tgcttatggt cacgtctccc ctcgcgcctc
     3121 ggtcgagttc gcccatctcg tcgatgtcca cctggaggac cctggccatg gcctcgagga
     3181 agcggccggt cccggcggcg cacttgtcgt tcatggcgaa gttcacg
//
LOCUS       DAOSCR010000300         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19121_length_3227_cov_3.282787, whole genome shotgun sequence.
ACCESSION   DAOSCR010000300 DAOSCR010000000
VERSION     DAOSCR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19121_length_3227_cov_3.282787"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLT69_07875"
     CDS             <1..583
                     /locus_tag="PLT69_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14391.1"
                     /translation="AIYGLGLAGPEQETARNGAAVHSAGVDPGEILELMHRADRLVQA
                     GDLEGAAVKYREALGRAPGYTDAYLKLGEALYRLGRYDEAASTFSQGLAKRPNDARLL
                     NNMGSVLLAKGDAAQALALFVQARRNSADYVEPLYNMACAYARMNRKDAALSSLGQAV
                     RMQPEVKLWAARDPDLASLRGEKEFEAIVNPKE"
     gene            603..2882
                     /gene="gspD"
                     /locus_tag="PLT69_07880"
     CDS             603..2882
                     /gene="gspD"
                     /locus_tag="PLT69_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOJ14392.1"
                     /translation="MKIRRVAGPVLLILLLCAGLAFGARLIQEETQPTVPKKVQTVGQ
                     DSQKGGAVQQANPAPGAARQPSVQTGEQTKAAGGRVPPQGGDQDARATARPQPAPAER
                     ETPAPRQDETAANGQFITMDFDGVDIKVFVKFIADITGKNFIIDEKVSGKVTVVSPRK
                     MTMDEAYRVFLSVLEVNGFGTVDVGGVTKIVKASDAITKSLQTTTEPPVARDDSMVTQ
                     IIQLKYADANDMRNLLSPLMSKASSQILAYPQSNVLIVTDVKSNIKKIIDILKVIDVA
                     GFAQEVRIIPLVYASAADLAAKLAEIISEEGSDQLQRLRTARQAESVGTKTSTKIIPY
                     ERTNTLIVMAPPQNLQGIEDLVKKLDIPTPTGKEDIHVYYLQYANAEDLAKVLTELPT
                     PENPEAQAQPASAAGGAAAQTLSKEQPRARTVANPALKQQNIKISPDKDTNSLIIYAD
                     PYQYRSIVETIKYLDIPRKQVYVSAAIMEVNATKDFKLGVEWTFAEDFDYDGGKRMGA
                     VVGRTGQSFVTSPGDLPSGPLVGVIGEAITLTSGSTKITFPNMASFINAMAKDSDVNI
                     ISTPQILTMDNKEAEIKVGANIPYVTREDTDSTNINRTVRTYDYRDVGVTLKLTPQIN
                     QQGDIRMNLFQEITTLVSSGSSNEYAPSTLKRSASTTVNVKDGSTMVIAGLIGDTLTL
                     ENYRVPLLGDIPILGYLFKSIGKKREKTNLYVFLTPRIIDTQEKTGRLYEEKAGEARK
                     SMGDKVQIPGTTSDEKAKP"
     gene            2863..>3227
                     /locus_tag="PLT69_07885"
     CDS             2863..>3227
                     /locus_tag="PLT69_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14393.1"
                     /translation="MRRQNLEQVLLEMQALDPSSLDRARELAREQGVGIATALSSAGL
                     VNEVDLLAALGRLWGLPVVHSISDLEVEPTVLDKLPVSFLRRYVMVPVRRQDGALVVA
                     VHDPLELEAFYDVGKALGV"
BASE COUNT          679 a         1052 c         1048 g          448 t
ORIGIN      
        1 cgcgatctac gggctcggcc tcgcgggccc cgaacaggag acggcccgca acggcgcggc
       61 ggtgcattca gccggcgtcg acccgggaga gatcctggag ctcatgcaca gggccgacag
      121 gctcgtccag gccggcgacc tcgaaggtgc ggcggtcaag taccgggagg ccctcgggcg
      181 cgcgcccggc tacacggacg cctacctgaa gctcggcgag gcgctctacc ggttgggcag
      241 gtacgacgag gccgcgtcga cgttctccca ggggctcgcc aagaggccga acgatgcgcg
      301 gctgttgaat aatatgggca gtgtgctatt agctaagggt gacgccgcgc aggcactcgc
      361 cctgttcgtc caggcgagga ggaactcggc ggactacgtc gagccgctct acaacatggc
      421 atgtgcctac gcacggatga acaggaagga cgccgccctt tcgtcgctcg ggcaggcggt
      481 acgcatgcag cccgaggtga agctctgggc ggcgagggat cctgacctgg cctcgttgag
      541 gggggagaag gagttcgagg cgatcgtgaa cccgaaggaa tgagggtcct caagggggag
      601 agatgaagat acgaagggta gcgggcccgg tcctgctcat cttgcttttg tgcgcgggcc
      661 tggccttcgg ggcgaggctc atccaggaag agacgcagcc gacggtcccg aagaaggtac
      721 agaccgtagg acaggactcg cagaaaggcg gcgccgtgca gcaggcgaac cctgcgccag
      781 gagccgcgag acagccgtcc gtccagacgg gagagcagac gaaggctgcg ggcgggaggg
      841 tgccgccgca ggggggagac caggatgcgc gggcgacggc ccggccgcaa cccgcgcccg
      901 cggagcgcga gacgccggcg ccccgccagg acgagaccgc cgcgaacgga cagttcatca
      961 ccatggactt cgacggcgtg gacatcaagg tgttcgtcaa gttcatagcg gacatcaccg
     1021 gcaagaactt catcatcgac gagaaggtct cgggcaaggt cacggtggtc tcgccgcgga
     1081 agatgaccat ggacgaggcc taccgcgtgt tcttgagcgt gctcgaggtc aacgggttcg
     1141 ggacggttga cgtgggcggc gtcacgaaga tcgtcaaggc atcggacgcc ataaccaaga
     1201 gcctccagac cacgaccgag ccccccgttg ccagggacga ctccatggtg acccagatca
     1261 tccagctcaa gtatgcggac gccaacgaca tgaggaacct cctctcgccc ctcatgtcca
     1321 aggcctcatc gcagatcctc gcctaccccc agagcaacgt gctcatcgtc acggacgtca
     1381 agtccaacat caagaagatc atcgacatcc tcaaggtcat cgacgtggcc gggttcgcgc
     1441 aggaggtgag gatcatcccc ctcgtgtacg cctctgccgc ggacttggcg gccaagctcg
     1501 ccgagatcat ctccgaggag ggatccgacc agcttcagcg gctcaggacg gcgcgccagg
     1561 cggagagcgt gggtacgaag acctcgacca agatcatccc ctacgagcgc acgaacaccc
     1621 tcatcgtcat ggccccgccc cagaacctcc agggcatcga ggacctggtc aagaagctcg
     1681 acataccgac gccgaccggc aaggaggaca tccacgtcta ctacctccag tacgcgaacg
     1741 cggaggacct ggcgaaggtg ctcaccgagc tcccgacgcc cgagaacccc gaggcgcagg
     1801 cccagcctgc ctcggccgca gggggcgccg cggcgcagac cctttccaag gagcagccca
     1861 gggccaggac cgtggcgaac cccgcgctca agcagcagaa catcaagatc agccccgaca
     1921 aggacacgaa ctccctcatc atatacgcag acccgtacca gtaccggagc atcgtggaga
     1981 ccatcaagta cctcgacata ccccgcaagc aggtgtacgt gagcgccgcg atcatggagg
     2041 tcaacgccac gaaggacttc aagctgggcg tcgagtggac cttcgccgag gacttcgact
     2101 acgacggcgg caagcgcatg ggcgccgtcg tgggcaggac gggccagtcg ttcgtcacga
     2161 gcccgggcga cctcccgtcg ggccccctgg tcggggtcat aggcgaggcg atcacgctca
     2221 ccagcgggag cacgaagatc accttcccca acatggcctc cttcatcaac gccatggcca
     2281 aggactcgga cgtgaacatc atctcgaccc cgcagatcct caccatggac aacaaggagg
     2341 cggagatcaa ggtcggcgcg aacatcccgt acgtcaccag ggaggacacg gactccacga
     2401 acatcaaccg gaccgtgcgc acctacgact accgcgacgt gggcgtgacc ctcaagctca
     2461 ccccccagat caaccagcag ggcgacatcc gcatgaacct cttccaggag atcaccacgc
     2521 tcgtgtcgtc gggctcatcg aacgagtacg ccccgtccac gctgaagagg tccgcgtcca
     2581 cgaccgtgaa cgtgaaggac ggctcgacca tggtcatcgc cggcctcatc ggcgacaccc
     2641 tgaccctcga gaattaccgg gtgccgctct tgggtgacat acccatcctc ggctacctct
     2701 tcaagtcgat aggcaagaag agggaaaaga ccaacctcta cgtcttcctg accccgcgga
     2761 tcatcgacac ccaggagaag accggcaggc tgtacgagga gaaggccggc gaggcgagaa
     2821 agagcatggg ggacaaggtg cagatccccg ggacgacgag cgatgagaag gcaaaacctt
     2881 gagcaggtcc tgctcgagat gcaggcgctc gacccctcct cgctcgacag ggcgagggaa
     2941 ctggccaggg agcagggcgt ggggatcgcg accgcgctgt cgtcggcggg cctcgtcaac
     3001 gaggtcgatc tcctcgccgc gctcggcagg ctctggggtt tgccggtcgt gcactccatc
     3061 tccgatctcg aagtcgagcc gaccgttctc gacaagctgc cggtaagctt cctcaggcgc
     3121 tacgtcatgg tgccggtccg caggcaggac ggggcgctcg tcgtggcggt gcacgacccg
     3181 ctcgagctcg aggccttcta cgacgtgggc aaggcgctcg gcgtcag
//
LOCUS       DAOSCR010000301         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19143_length_3225_cov_3.904101, whole genome shotgun sequence.
ACCESSION   DAOSCR010000301 DAOSCR010000000
VERSION     DAOSCR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19143_length_3225_cov_3.904101"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..579)
                     /locus_tag="PLT69_07890"
     CDS             complement(301..579)
                     /locus_tag="PLT69_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOJ14394.1"
                     /translation="MRRKKPDPERAVALKYERGRDPAPRVTAKGSGAVAAKILELARK
                     HGVPIEKDPALLDALYRLDLNEEIPEELYRVVAEILAFVYRMNALKRA"
     gene            complement(576..1343)
                     /locus_tag="PLT69_07895"
     CDS             complement(576..1343)
                     /locus_tag="PLT69_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14395.1"
                     /translation="MTVTQITAAKQGVILLRDAVPSLAVGRQVVATVLSAPKDGMVLV
                     SMFGRRILVETTLELFEGQVLRLRVHELEPRVVMKPEAAPASARAAQEDVEALVARLA
                     GRFPQAEVRSFDLREIVKTLLREGKDDPKVIWQAKTLIEEFARLAGLPAAFFLVPVVD
                     DQGRSKARISAEKTDDGYRLHFDVETDALGIVECTALRNRAGIHVEISSAHQEVVSLM
                     KAHLHELASALGDMGVTTLEVVRRAHAVREPTVDMLV"
     gene            complement(1358..1804)
                     /locus_tag="PLT69_07900"
     CDS             complement(1358..1804)
                     /locus_tag="PLT69_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14396.1"
                     /translation="MKVRIFSSDPLYLLLAVQMVIQIVLVFFVVLLLVVEKRRRMSAD
                     ALEELRKVVDQTRELSQAFQENVQQRVDVLGRLMAELDDKTASARRLVNALEEVSVKA
                     KRSRQFGQEDVKKLHKGGYDPLEISQITGIPVGEIQLMIKVGEPRT"
     gene            complement(1801..2127)
                     /locus_tag="PLT69_07905"
     CDS             complement(1801..2127)
                     /locus_tag="PLT69_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14397.1"
                     /translation="MAAGPSDISVILSQAGRIEKITQNTFVQSEVAKQILTEQEAKER
                     LQRTREVNQPPKAHEITISEKAKQESMRHAKDSGARSAPDETPGTETAQEEAGPENKH
                     IIDVVV"
     gene            complement(2319..2687)
                     /locus_tag="PLT69_07910"
     CDS             complement(2319..2687)
                     /locus_tag="PLT69_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOJ14398.1"
                     /translation="MTLAEVRDILDAEVIVGEDKLDMIVERGFAADLMSDVLAFAKEG
                     SMLLTGLTNPLVVRTADTLDLSVIVFVRGKRPSPEAVKLAQEKNIPLLATRYILFESC
                     GRLYGAGMRGSIVKVGEPRD"
     gene            complement(2716..>3225)
                     /locus_tag="PLT69_07915"
     CDS             complement(2716..>3225)
                     /locus_tag="PLT69_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ14399.1"
                     /translation="VRTAMRLGADHVYLIYRRSRAEMPARIEEIHHAEEEGIEFHLLT
                     NPVRYIGDDKGWVVGVECLKMELGEPDESGRRRPVPVKGSEFVIDVDTVVVAIGAGAN
                     PLVQSTTPGLETNKWNYIVADEETGQTSREGVFAGGDIVTGAATVILAMGAGRKAARA
                     MHEYLMSKK"
BASE COUNT          529 a         1090 c          966 g          640 t
ORIGIN      
        1 cgagatccgc cggatacgtc caccaggcaa actcttgcaa gacgcacctc tccgtccgtc
       61 cacccgatcg ccgagactcc tccatgaagg gcgccaatcc tcggcttgga tgcgctcacc
      121 gagaagacaa tcacacccgg agacgatcgg gcggcgccac cacccatccc tgcagggggc
      181 tgcagaacag tttgatccct tggtgcggcg atctcgccgg catgcgggct tgtcgcgatc
      241 agggagacag gtgatgcgca ccgccccggt ctctccatcc aggcatgggc gcgcgcaggt
      301 tcaggccctc ttgagcgcgt tcatcctgta gacgaaggcg aggatctcgg ccaccacgcg
      361 gtagagctcc tccgggatct cctcgttcag gtccagccgg tagagggcgt cgaggagggc
      421 ggggtccttc tcgatcggaa caccgtgctt cctcgcgagc tcgaggatct tcgcggcgac
      481 ggcgccgctg cccttggccg tcaccctcgg ggcggggtcc ctgccccgtt cgtacttgag
      541 ggcgacggcc ctctcagggt cgggcttctt ccttctcata cgagcatatc cacggtgggc
      601 tcacgaacgg catgcgccct gcgcaccacc tccagggttg tcacgcccat gtccccaagg
      661 gccgacgcga gctcgtggag gtgggccttc atgagggaga cgacctcctg gtgggcggac
      721 gagatctcca cgtggatacc cgcccggttc cttaaagccg tgcactccac gatccccagc
      781 gcgtcggtct ccacgtcgaa gtgcagccgg tacccgtcat cggttttctc tgcggagatc
      841 ctcgctttcg acctgccctg gtcgtcgacg acgggcacca ggaaaaacgc cgccggaagg
      901 cccgcaagcc tggcaaactc ctcgatgagg gtctttgcct gccagatcac cttcgggtcg
      961 tccttcccct cccgcaggag ggtcttcacg atctccctga gatcgaaaga cctcacctcg
     1021 gcctggggga agcgtcccgc caggcgcgcc accagggcct cgacgtcttc ctgtgccgcc
     1081 cgggcggaag cgggagccgc ctccggcttc atgaccaccc ggggttcgag ctcgtggacc
     1141 ctgagcctca agacctgtcc ctcgaacagc tccagggtgg tctcgaccag gatgcgcctg
     1201 ccgaacatgg acacgagcac catgccgtcc ttcggggccg acagcaccgt cgcaacgacc
     1261 tgcctgccca ccgcgagaga ggggaccgcg tctctgagga ggatgacgcc ttgcttggcg
     1321 gcggtgattt gcgtgacggt catggggtgt cacaccttca ggtgcggggt tccccaacct
     1381 tgatcatgag ctggatttcc cccaccggga tgcccgtgat ctgggagatc tccagcgggt
     1441 cgtacccgcc cttgtgcagt ttcttcacgt cctcctggcc gaactgccgt gacctcttcg
     1501 ccttcacgga gacctcttcg agggcgttga cgagtcgcct ggcggatgcg gtcttgtcgt
     1561 cgagttcggc catgagcctg cccaagacgt ccacacgctg ctgcacgttc tcctggaagg
     1621 cctgggagag ctccctcgtc tggtcaacga cctttctgag ctcttcgagg gcgtcggcgc
     1681 tcatcctgcg cctcttctcg accacgagga ggaggacgac gaaaaagacc aggacgatct
     1741 gtatgaccat ctggaccgca aggagcaggt acaagggatc cgaggaaaag atcctcacct
     1801 tcacaccacc acgtctatga tgtgcttgtt ctccggcccg gcctcttcct gggccgtctc
     1861 ggtcccgggc gtctcatcgg gagcggaccg tgccccggag tccttcgcgt gtctcatgct
     1921 ctcctgcttg gccttctcgc tgatggtgat ctcgtgggcc ttgggcggtt ggttcacctc
     1981 gcgcgtgcgc tggagcctct ccttggcctc ctgctccgtg aggatctgct tggcgacctc
     2041 cgactgcacg aaggtgttct gcgtgatctt ctcgatcctc ccggcctggg agaggatcac
     2101 ggagatgtcg gaaggtcctg cagccatgct ctgctccctc agcacaccta tcggtatcac
     2161 catataccaa cttaagcttg catatgcaaa caccgcccgc gcgcaaggct gcttgcacta
     2221 caacggaagg ggcacctgcg cgaaaggcct gtccgcgtaa gggcgatggc caggcgggcg
     2281 cccgggcgaa tggatccttg gaaggacgag ggggagcgct aatcccgcgg ctcccccacc
     2341 ttgactatgg agcccctcat gccggcgccg tagagtctcc cgcaggactc gaagagtatg
     2401 tacctggtcg cgagcagcgg gatattcttc tcctgggcga gtttcacggc ctcgggggag
     2461 gggcgcttgc ccctcacgaa gactatgaca ctgaggtcca gggtatctgc cgtcctgacc
     2521 acgagcgggt tggtgagccc tgtcaggagc atggatcctt ccttggcgaa ggcgaggacg
     2581 tcgctcatga ggtctgcggc aaacccgcgt tccacgatca tgtcgagctt gtcctccccc
     2641 acgatcacct ctgcgtcaag gatatctctc acttcggcaa gggtcatacc ctctcctctc
     2701 tcaacgaaac gaatctcatt tcttggacat caggtactcg tgcatcgccc tcgcggcctt
     2761 cctgccagca cccatcgcga ggatgaccgt cgccgcgccg gtgacgatgt cgccgcctgc
     2821 aaatacaccc tcgcgtgagg tttgtccagt ctcttcgtcc gcgacgatgt aattccactt
     2881 gttcgtctcg aggccgggcg tggtgctctg gacaagcggg ttcgcgcctg ccccgatcgc
     2941 gaccaccacc gtgtcgacgt cgatgacgaa ctcgctcccc ttgacgggaa ccggccgcct
     3001 gcggccggac tcgtcgggtt ccccgagttc catcttgagg cattctaccc caacgaccca
     3061 ccccttgtca tcgcctatgt aacgtaccgg gttggtcagg agatggaact cgatcccctc
     3121 ctcctctgca tggtggatct cctcgatcct ggccggcatt tccgcgcggg agcggcggta
     3181 gatgaggtag acatggtcgg cgccgagcct catggcggtc ctcac
//
LOCUS       DAOSCR010000302         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19156_length_3224_cov_3.450300, whole genome shotgun sequence.
ACCESSION   DAOSCR010000302 DAOSCR010000000
VERSION     DAOSCR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19156_length_3224_cov_3.450300"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PLT69_07920"
     CDS             <1..865
                     /locus_tag="PLT69_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ14400.1"
                     /translation="HKGVTVVTDLEEGLLPVMGSTIHIRKALMNLVLNAMEAMPEGGT
                     VTLTTRNAFLEGPIEGHDDIFPGDYVVLTVRDTGVGIDPADMRRIFEPFYSKKVLGRS
                     GTGLGMAVVWGTVQDHRGYINVKSTPEQGSSFELFFPATRMEAVESQERASQEDIAGN
                     GELILVVDDLDTQRQIATDILTRLGYTALSAASGEEALGLLRETAVDCVLLDMIMEPG
                     MDGLDTYRRILEINPGQKALIASGYAENERVREAIRLGASGYLKKPYTIENLGLSLKN
                     CLAAGDGGSPA"
     gene            complement(1264..2175)
                     /locus_tag="PLT69_07925"
     CDS             complement(1264..2175)
                     /locus_tag="PLT69_07925"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOJ14401.1"
                     /translation="MKLEEVKKVLIIGAGTMGQQIGLQCAMHGYETVYYDLSRQILDK
                     AMERVARLARWYESTGRIPAGGLEEVLARISSSADPKEAATDVDFVSESVPEDPELKA
                     KVFAQFNELCPERTVFTTNTSMLVPSMFAEASGRPDRLCALHFHDLRATTVVDVMPHE
                     GTSPEVTELVRAFARSIGCIVIMLHRENNGYVFNTMLSSLFFSALTLASRGVASVEDI
                     DRAWMGVTYMPMGPFGIMDQVGLSTVWTITDYWAKRTKDPQAQMNADFLKPYVERGDL
                     GYKTGKGFYTYPNPAYQRQGFLDGVGS"
     gene            complement(2205..2969)
                     /locus_tag="PLT69_07930"
     CDS             complement(2205..2969)
                     /locus_tag="PLT69_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ14402.1"
                     /translation="MEKIDFRLDGKIALVTGASRGIGEAIAVTLADYGAHCILVSRKE
                     EGLKKVSETIAQRGGKAEVMACHVGDLPQIERLFARIQERHGKLNILVNNAATNPYFG
                     EMIGADEGIWNKTFDVNLKGPFFLVKHAVPLMTASGGGSIVNVSSINGVRPAPYQGIY
                     SITKAGLISMTQAFAKELAARNIRVNALLPGLVETHFSKAILENDAIREYAVKMIPMG
                     RHAVPREIAGVVLYLVSDAASFTTGACFVVDGGALA"
BASE COUNT          552 a         1097 c         1005 g          570 t
ORIGIN      
        1 ccacaagggt gtcacggttg tcactgacct cgaagaaggg cttctgcccg tcatgggatc
       61 aaccatccac atccgcaagg ccctcatgaa cctggtcctc aacgccatgg aggccatgcc
      121 ggaggggggc accgtgaccc tcacgacgcg caacgccttc ctggaaggcc cgatagaggg
      181 ccatgacgac atcttccctg gcgactacgt ggtcctcacc gtccgggaca cgggtgtggg
      241 gatagatccg gcggacatga ggcgcatctt cgagccgttc tactcgaaga aggtcctggg
      301 acggagcggc acggggctcg gcatggccgt ggtgtgggga accgtccagg accatcgcgg
      361 gtacatcaac gtcaagagca cacccgaaca ggggtcgtcc ttcgagctct tcttcccggc
      421 cacgagaatg gaggccgttg aatcccagga aagggcgtcc caggaggaca tagcaggcaa
      481 cggcgagctc atcctcgtgg tggacgacct cgacacgcaa cgccagatag caaccgacat
      541 cctcaccagg ctggggtaca cggcgctgag cgcggccagc ggcgaagagg cgctgggtct
      601 cctgagggag acggccgtgg actgcgtgct gctcgacatg atcatggaac cgggcatgga
      661 cggcctcgac acgtaccggc ggatcctcga gatcaacccc ggccagaagg ccctcatcgc
      721 gagcgggtac gcggagaacg agcgggtgag ggaggcgata cggctcggcg cgagcggcta
      781 cctcaagaaa ccctacacca tcgagaacct gggcttgtct ctcaagaact gcctcgccgc
      841 aggcgacggg ggctctcccg cctgatcccg agacgggcgc aggacgactc gcccccggcg
      901 gtcgcatcag ggaaggggcg tcctttcccg caacgtccag gcgtcttcat gcagcggcgc
      961 gatcaatccc ttccgccccg ctcaggccag ggcatccacc cgccgcctat ccactgaacg
     1021 agggggacca tcgtgagcgt ccccggcccc gggacaacca cccaagaccg agctgggggg
     1081 cggggttcca ggcctgtccg cctgaccggg agaccgcgcc ccggcaaggg cagcccacgc
     1141 ccggggcgcc tatccacgcc gcgcgggtga ccgctcatgg atagggaggg cacgggccct
     1201 tcccgcccgc ggctaccctg atcccccctg tgggagactg cgtgccctcc acgggccttc
     1261 gtgctaggag cctacgccgt cgaggaaccc ctggcgctgg taggcagggt tcgggtaggt
     1321 gtagaacccc tttcccgtct tgtagccgag gtctcccctc tcgacgtacg gcttgagaaa
     1381 gtcggcgttc atctgcgcct gggggtcctt cgtcctcttc gcccagtagt ccgtgatggt
     1441 ccacaccgtg gaaagcccca cctggtccat gatgccgaag gggcccatgg gcatgtaggt
     1501 cacccccatc cacgccctgt ctatgtcctc cacggaggcg accccccggg acgcgagcgt
     1561 gagggcggag aagaagaggc tcgagagcat ggtgttgaac acgtagccgt tgttttccct
     1621 gtggagcatg atcacgatgc acccgatgct cctggcgaac gccctgacga gttccgtcac
     1681 ctcgggcgag gtgccctcgt ggggcatgac gtcgacgacg gtggtggccc tgaggtcgtg
     1741 gaaatggaga gcgcagagcc tgtccggcct gcccgaggcc tcggcgaaca tggaagggac
     1801 gagcatcgac gtgttggtgg tgaacacggt ccgctctgga cacagctcgt tgaactgcgc
     1861 gaagaccttc gccttgagct ctgggtcctc gggcacggat tcgctcacga agtccacatc
     1921 cgtggccgcc tctttcgggt cggcactcga ggagatcctt gccagcacct cctcgagccc
     1981 tcccgccggg atcctccccg tcgactcgta ccacctcgcg agcctggcca ccctttccat
     2041 cgccttgtcg aggatctggc ggctcagatc gtagtacacg gtctcgtacc cgtgcatggc
     2101 gcactgcagg cctatctgct gccccatggt accggcgcct atgatgagca cctttttcac
     2161 ctcttcgagc ttcatggatg tctctccttt cccgtcgaag gcgctcacgc gagcgcgccg
     2221 ccgtccacca cgaagcacgc cccggtcgtg aacgaggcgg cgtccgaaac gaggtagagc
     2281 accacgcccg cgatctccct cggcacggcg tgcctgccca tgggtatcat cttgacggcg
     2341 tactcccgta tcgcgtcgtt ctcgaggatg gccttcgaga agtgcgtctc cacgagcccc
     2401 gggaggaggg cgttcaccct gatgttgcgc gcggcgagct cctttgcgaa ggcctgggtc
     2461 atggatatga ggccggcctt ggtgatcgaa tagatgccct ggtacggggc cggcctgacc
     2521 ccgttgatgg acgacacgtt cacgatggag cccccgcccg atgccgtcat caggggcacg
     2581 gcgtgcttga ccaggaagaa cgggcccttc aggttcacgt cgaaggtctt gttccagata
     2641 ccctcgtcag cgccgatcat ctcgccgaaa tacgggttgg tcgcggcgtt gttgacgagg
     2701 atgttgagct ttccgtgcct ctcttgtatc cgagcgaaga gccgctcgat ctgcggcaga
     2761 tccccgacgt gacaggccat cacctcggcc ttgccgcccc tttgtgctat ggtctccgag
     2821 accttcttca acccctcttc cttgcggctc acgaggatac aatgcgcccc gtagtcggcg
     2881 agggtaacgg ctatggcctc cccgatgccc ctgctcgccc cggtcaccag ggcgatcttc
     2941 ccgtccagcc tgaaatcgat cttctccatg gtctccttct cccgttcacg tagtgtgccc
     3001 gtctcgcccg gtcccgaaca ggacacggcc ggtcaatccc gtaaagggca tccatcggcc
     3061 ggcccgcggc gtccaccgcc tgggccccaa agagcgccgg ccctcctcgc ttgttcgccg
     3121 cggcatggag gggaacccct tgcgcccggc gccgaccttc acccccaacc ctcctccatc
     3181 gccctcttgg aagccttctc gaggatgtgg atggcgaaga tcag
//
LOCUS       DAOSCR010000303         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19232_length_3215_cov_3.390506, whole genome shotgun sequence.
ACCESSION   DAOSCR010000303 DAOSCR010000000
VERSION     DAOSCR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19232_length_3215_cov_3.390506"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PLT69_07935"
     CDS             complement(<1..164)
                     /locus_tag="PLT69_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ14403.1"
                     /translation="MFSGAIVAIVTPFDGGGIDEPAYARLIEDVIAGGISEIVPCGTT
                     GESATLTHEE"
     gene            complement(146..1438)
                     /gene="lysA"
                     /locus_tag="PLT69_07940"
     CDS             complement(146..1438)
                     /gene="lysA"
                     /locus_tag="PLT69_07940"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOJ14404.1"
                     /translation="MHDFEYNASGELCCGALPLRAIAEEVGTPFYCYSLPTLKRHIRA
                     FEEPLAGIDHQTCFAMKANSNMAVLALMAKEGLGADVVSGGELFRALKAGIPATKIVY
                     SGVGKTTEEIDMAISSGIMMFNIESSEELSVINERAGVLGRKAPVSIRVNPDVDPQTH
                     PYISTGLKKNKFGIDIEASVRQYQAASRMANIEIVGIDCHIGSQLTDIAPFIDAIERL
                     KTLISDLRRLGIGFSYLDLGGGLGITYSTEQPPHPREYGAAVIEAVKGLDLKLVFEPG
                     RVIVGNAGVLVTRVLYRKETAEKTFIIVDAGMNDLIRPALYNAWHEIVPLEKKSGPKE
                     RVDVVGPICESSDFLAKDRDLVRLGRGDLIAVMSAGAYGFTMASNYNSRPKPPEVLVD
                     GERSCVVVRRQSYEDLVAGEVIPDEMFERGETRCSQVR"
     gene            complement(1460..1852)
                     /locus_tag="PLT69_07945"
     CDS             complement(1460..1852)
                     /locus_tag="PLT69_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14405.1"
                     /translation="MAREAVLVVLAISILVACTGCGRKLPPLPPEQVEPARISDAWYE
                     RGEVVVRVTCTVAGKVTLLGKAVGDCPQCLEGLAPKDEQTVDAPRSVELRDREPAAGP
                     MVYRVRVVAAGGAWTGPPRIVEGPRANP"
     gene            complement(1842..>3215)
                     /gene="argH"
                     /locus_tag="PLT69_07950"
     CDS             complement(1842..>3215)
                     /gene="argH"
                     /locus_tag="PLT69_07950"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOJ14406.1"
                     /translation="GRFSQETEKLVERFTSSVDVDARLYLEDIEASKAHARMLGRRGI
                     LGEAEVEAILGGLDAIRQDIESGSFVFDPGLEDVHMNIEVELTRRIGEAGKKLHTARS
                     RNDQVVTDLRLYTRKALERIASGLESFMDAVLDKAAEHVDTIIPGMTHLQHAQPVSLA
                     HHLLAYFEMALRDRERVSQVMERVNVMPLGSAALAGTPHGIDRLSTAYDLGFTAVARN
                     SIDAVSDRDFALEATFLCAVIMMHLSRLAEEIVIWNSQPFSFVELPDAYCTGSSIMPQ
                     KKNPDVAELVRGKTGTVYGDLVALLTMMKGLPLAYNRDMQEDKGPLFRSLDTTCACLD
                     VMAGLVRGMEPNGPGIERSLKEGFITATDLADYLASRGMPFRDAHRVTGEIVSHCLRT
                     SKVFSDLTLEDLKAFSPLFEPGVFEVIDPRVSVDRRTSMGGTARPNVRAALDEARAIL
                     EGIRRGA"
BASE COUNT          521 a         1106 c          973 g          615 t
ORIGIN      
        1 tgctcctcgt gagtcagcgt cgccgactcc ccggtggtcc cgcacggcac tatctcgctg
       61 atgccgcctg cgatcacgtc ctctatcaga cgcgcgtacg caggctcgtc tatcccgccg
      121 ccgtcaaagg gcgtgacgat ggcgactatc gcacctgaga acatcgagtt tcccccctct
      181 cgaacatctc gtccgggatg acctctccgg cgacgagatc ctcgtaagac tgcctcctta
      241 ctaccacaca tgatctctcg ccatccacca gaacctcggg cggcttgggc ctcgagttgt
      301 agttcgacgc catggtgaat ccgtaggccc ccgcgctcat gaccgcgatg aggtccccgc
      361 ggccgagccg cacgagatcc ctgtctttcg ccaggaaatc gctcgactcg catatgggcc
      421 ccaccacgtc gaccctctcc ttgggaccgg acttcttctc gagggggacg atctcgtgcc
      481 aggcgttgta gagcgcgggc ctgatgaggt cgttcattcc tgcatcgaca atgatgaagg
      541 tcttttcagc ggtctccttc cggtacagca cgcgcgtgac gagaacgccc gcgttgccga
      601 cgatcaccct gccgggctcg aacacgagct tcaggtccag ccccttcacc gcctcgatca
      661 ccgcggcgcc gtattcccgc gggtgcggag gctgctccgt cgagtacgtt atgccgagcc
      721 cgccgcccag gtcgaggtag gaaaacccta tcccgaggcg cctcaagtcg cttatgaggg
      781 tcttgaggcg ctctatggcg tcgatgaagg gtgcgatatc cgtgagctgg ctgccgatgt
      841 gacagtctat gcccacgatc tctatgttgg ccatgcggct ggccgcctgg tactgcctca
      901 cggatgcctc gatgtctatg ccgaacttgt tcttcttcag gccggtggag atgtacgggt
      961 gggtctgcgg atccacgtca gggttcaccc tgatggagac gggcgccttt ctccccagga
     1021 cgcccgcccg ctcgttgatg acagagagct cctcggagct ctctatgttg aacatcatga
     1081 tgcccgagga gatcgccatg tcgatctcct cggtcgtctt gcccaccccg gagtacacga
     1141 tcttcgttgc ggggatcccg gccttgagcg cgcggaagag ctccccgccc gagaccacat
     1201 cggcgccgag cccctccttc gccatgaggg cgagcacggc catgttcgag ttggccttca
     1261 tggcgaagca cgtctggtga tcgatgccgg cgagaggctc ctcgaaggcc cgtatgtgcc
     1321 tcttgagcgt cggcaggctg tagcagtaga acggggtgcc gacctcttct gctatcgctc
     1381 gaagaggcaa cgcaccgcag cacagctctc ccgaggcgtt gtactcgaag tcatgcatga
     1441 acgacgttcc cctttcctcc tacggattcg ccctgggccc ctccacgata cggggcggcc
     1501 ccgtccaggc tcccccagcg gcgaccaccc tgaccctgta caccatgggg ccggcggccg
     1561 gttccctgtc cctgagctcg accgaccgcg gcgcatcgac cgtctgctcg tcctttggcg
     1621 ccaggccctc caggcattgg gggcagtccc ccacggcctt gccgagaagg gtcaccttcc
     1681 cggccacggt gcacgtgacg cgcaccacga cctcgcctcg ttcgtaccac gcatccgaga
     1741 tgcgggccgg ctccacctgt tcggggggca agggggggag cttcctgccg cagccggtgc
     1801 aggcaacgag gatcgagatc gccaagacga cgagtacggc ctcacgcgcc acgacggatc
     1861 ccctcgagga tcgcgcgggc ttcgtcgagg gccgcccgga cgttgggcct cgccgtcccg
     1921 cccatgctcg tcctcctgtc gaccgagacc cgagggtcga tcacctcgaa cacgcccggc
     1981 tcgaagaggg gggagaacgc cttgagatcc tccagggtga ggtcggagaa caccttggag
     2041 gttcgcagac agtgcgagac gatctccccc gtcaccctgt gcgcatccct gaagggcatc
     2101 cccctcgagg ccaggtaatc ggcgaggtcg gtggccgtga tgaagccctc tttcagggac
     2161 cgctcgatgc ccggcccgtt cggctccatg cccctgacga gcccggccat cacgtcgagg
     2221 cacgcacagg tggtgtcgag gctcctgaag agcggcccct tgtcctcctg catgtcccgg
     2281 ttgtaggcca ggggaaggcc tttcatcatg gtgagcagcg ccacgagatc tccgtacacg
     2341 gtgcccgtct tgcccctgac gagctcggcc acgtcagggt tcttcttctg gggcatgatg
     2401 gagctgcccg tacagtaggc gtccggcagc tccacgaagg agaagggctg ggagttccag
     2461 atgacgatct cctccgcgag cctgctcaag tgcatcatga tcacggcgca caaaaaggtc
     2521 gcctccaggg cgaagtccct gtcgctcacg gcatcgatcg agttccgtgc cacagccgtg
     2581 aacccgagat cgtacgcggt cgagagcctg tctataccat ggggtgtgcc ggccagcgca
     2641 gcgctgccca gcggcatcac gttgaccctc tccatgacct ggctcacccg ctcgcggtcc
     2701 ctgagcgcca tctcgaagta cgccagcagg tgatgggcga ggctcacggg ctgcgcatgc
     2761 tgcaggtggg tcatccccgg gatgatcgtg tccacgtgtt ctgcggcctt gtcgaggacc
     2821 gcgtccatga acgactccag tcccgatgcg atcctttcca gcgccttgcg cgtgtagagc
     2881 ctgagatcgg tgaccacctg gtcgttcctg ctgcgcgcgg tgtggagttt cttccccgcc
     2941 tccccgatcc tcctggtcag ctcgacctcg atgttcatgt gcacgtcctc gaggccggga
     3001 tcgaagacga acgagccgct ctctatgtcc tgcctgatgg cgtcgagccc gccgaggatg
     3061 gcctccacct cggcctcacc gagaatgccg cggcggccga gcatcctcgc gtgcgccttg
     3121 ctcgcctcga tgtcctcgag gtagagcctt gcgtccacgt ccaccgaaga cgtgaacctc
     3181 tcgacaagct tctcggtctc ctgggagaag cggcc
//
LOCUS       DAOSCR010000304         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19234_length_3215_cov_3.274051, whole genome shotgun sequence.
ACCESSION   DAOSCR010000304 DAOSCR010000000
VERSION     DAOSCR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19234_length_3215_cov_3.274051"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLT69_07955"
     CDS             complement(<1..231)
                     /locus_tag="PLT69_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HOJ14407.1"
                     /translation="MIIKTPTTYFMTSGSSEGFTKLNAFDGALMAAGIGNTNLVKMSS
                     ICPPHTTQVDSVNLPHGALVPVAYASITSDRPG"
     gene            complement(251..793)
                     /locus_tag="PLT69_07960"
     CDS             complement(251..793)
                     /locus_tag="PLT69_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ14408.1"
                     /translation="MKIGEKIKNLRLAQELTQEELANRADLTKGFISLLERDLQSPSL
                     DTLELILNALDTNPKEFFSDEGEEVVVFHRSQRVSITDGDEPGVKREVLIAGAQDREM
                     DLLLVTLKPGASTYQERAHYGDECGYVLTGKIVINIGKETYKASAGDTFYYTANRKHW
                     IENKSDKPAKILWISAPPSF"
     gene            1074..1301
                     /locus_tag="PLT69_07965"
     CDS             1074..1301
                     /locus_tag="PLT69_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14409.1"
                     /translation="MIEKIPFIHRQRLYNIVIEEDITFTALHVIIDDLIGQGAFEAER
                     DGGDLYLVEHADVRYTVGVDGIDVIITVQGH"
     gene            1312..2163
                     /locus_tag="PLT69_07970"
     CDS             1312..2163
                     /locus_tag="PLT69_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14410.1"
                     /translation="MSTSIHLCQPAENVSCAACCGIYNYARNRRTELVKRFENRTRLF
                     GLVRQGKLGLEGYRGIVKRSEHLKRVFPTIYSCEFVGYLDDGRRRVGCMLHPASNNGE
                     DLRDASFYGRDICDGHFCPSYAKLEDHEVRVILSCVDDWYLYGALITDIDFVKNFFDS
                     VQNRLGESVDPARVSPGSAPAAIFRRLCGLKLDWPYRDTSRPRFGKYFFVGEDYDIAR
                     IDYASLGAPRSPYDPILVSLASSFKNKDELTSAHLIIENLVEGFCSAYERSRGQDHPD
                     GPRGRRP"
     gene            2120..2935
                     /gene="nadC"
                     /locus_tag="PLT69_07975"
     CDS             2120..2935
                     /gene="nadC"
                     /locus_tag="PLT69_07975"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOJ14411.1"
                     /translation="MDRIILMALEEDGRDITSEAIFRVPQTLSAVVRAKAEGVVAGIE
                     VARRTFLLVDPGIVFDAACKDGDRVSRGSIVAKVRGPALGVLKAERVALNFLQRMSGI
                     ATLTSEYVRRVEGTRAKILDTRKTAPGMRILDKAAVRCGGGCNHRMGLFDMALVKDNH
                     VDAAGSIEEAVHRVRDAFPGVPVEVEARTLEDVGLLLKLGVDRIMLDNFSLQDTKRAV
                     ALVSGRVPLEASGNITLDRVREVAETGVDFISVGEITHSVRALDIAMTIEVSS"
     gene            2932..>3215
                     /gene="nadA"
                     /locus_tag="PLT69_07980"
     CDS             2932..>3215
                     /gene="nadA"
                     /locus_tag="PLT69_07980"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOJ14412.1"
                     /translation="MNAIEPAAVIRRARGRYGRRLLILAHHYQADEVVAHADFVGDSL
                     ELARRAAREKEAEFIVLCGVHFMAETAAILSPGKKVYIPDPQAGCPLADSA"
BASE COUNT          579 a          994 c         1021 g          621 t
ORIGIN      
        1 gcccggcctg tcggacgtga tcgacgcgta ggccaccggc acgagggcgc cgtgcgggag
       61 gttcacggaa tccacctgtg tcgtgtgggg ggggcatatg ctgctcatct tcacgaggtt
      121 cgtgttgccg ataccggcgg ccatgagcgc accgtcgaaa gcattcagct tcgtgaaccc
      181 ctccgacgaa cccgaggtca tgaagtacgt ggtaggcgtc ttgatgatca tgggtcatcc
      241 cccctttctt tcagaaactc ggcggtgccg agatccagag gatcttcgcc ggcttgtccg
      301 acttgttctc tatccagtgt ttcctgttcg ccgtgtagta gaacgtgtcc ccggccgagg
      361 ccttgtacgt ctccttcccg atgttgatca cgatcttgcc ggtcaggacg tacccgcact
      421 cgtctccgta gtgcgccctt tcctggtagg tgctggcgcc tggcttcagg gtcacgagca
      481 ggaggtccat ctctcggtcc tgtgcaccgg cgatgagcac ctcgcgcttg acgcccggct
      541 cgtcgccgtc ggtgatggag accctctgcg agcggtggaa gacgacgacc tcttccccct
      601 cgtctgagaa gaactccttg gggttcgtgt cgagggcgtt caggatgagc tccaaggtgt
      661 ccagcgaggg cgactggagg tccctctcga ggagcgagat gaagcccttg gtgaggtccg
      721 cgcggttcgc gagctcctcc tgcgtaagct cttgagcgag cctcaggttt tttatcttct
      781 cccctatctt catccgctct cctaagacac tttagtgtac cttcctgttt actctagtaa
      841 accgagttgg gggtctcttt aaggtatcct gtccctggtg tcaagcactt ttttcgcccg
      901 gggaggcagg gtgtcggtac aaggggggcc gtgtccccag gcgagccgaa cgctcgaggt
      961 aaggaccctc ctgccggtct ggtcgtggcg accgggccga gttccaccgc gtacaggacc
     1021 agcggtctct tccgtcttca agaatgcgct gacctgtggt attgtccggt gagatgattg
     1081 agaagatccc gttcattcac aggcagcgcc tgtacaacat cgtgatagag gaagacatca
     1141 ccttcacggc gctccacgtt atcatcgacg acctcatcgg ccagggggcc ttcgaggccg
     1201 agcgggacgg cggcgacctc tacctggtcg agcacgccga cgtgcgctac acggtgggtg
     1261 tcgacgggat agacgtgatc atcaccgtcc agggccactg acgacctccc catgagcacc
     1321 tccatacacc tgtgccagcc cgcagagaac gtctcgtgcg ccgcgtgctg cggcatctac
     1381 aactatgcca ggaacaggag gacagagctc gtcaagcgat tcgagaaccg gacgaggctc
     1441 ttcgggctcg tgaggcaagg gaagctcggc ctcgaggggt accgcggcat cgtgaaacgc
     1501 agcgagcacc tcaagcgtgt cttccccacc atctactcct gcgagttcgt cggatatctc
     1561 gacgacggcc gcaggcgcgt gggatgcatg ctccacccgg cgtccaacaa cggcgaggat
     1621 ctcagggatg cgagcttcta cggcagggat atctgcgacg gccacttctg cccgagctat
     1681 gcgaagctcg aggaccacga ggtccgcgtg atcctctctt gcgttgacga ctggtacctc
     1741 tacggcgctc tcatcacgga catcgacttc gtgaagaact tcttcgactc ggtccagaac
     1801 aggctcggtg aatccgtgga ccccgcaagg gtgtcgccgg gaagcgcccc tgccgccata
     1861 ttcaggcgcc tgtgcggcct gaagctggac tggccctaca gggacacatc gagaccccgc
     1921 ttcgggaagt atttcttcgt gggcgaggat tacgacatag ccaggataga ctatgcctcg
     1981 ctgggcgccc ccaggtcacc ctacgacccg atccttgtga gcctcgcctc atccttcaag
     2041 aataaggatg aactcacgag cgcacatctg ataatagaga acctcgtgga ggggttctgc
     2101 agtgcatacg agagatcccg tggacaggat catcctgatg gccctcgagg aagacggccg
     2161 tgacatcacc tcggaggcga tattccgcgt cccacaaacg ctgagcgcag tcgtgcgggc
     2221 caaggccgag ggggttgttg caggtatcga ggtcgccagg cgtaccttcc tgctcgtcga
     2281 cccgggtatc gtcttcgatg cggcctgcaa ggacggcgac agggtatccc gtggatccat
     2341 cgtcgccaag gtcagggggc ctgccctcgg tgtgctcaag gccgagcgcg tcgccctgaa
     2401 cttcctccag cgcatgagcg ggatcgccac gctcacttcc gagtacgtca ggagggtcga
     2461 gggtaccagg gcgaagatcc tggacacccg caagaccgcc cccgggatgc gcatcctcga
     2521 caaggccgcg gtgaggtgcg gcggggggtg caaccaccgc atggggctgt tcgacatggc
     2581 gctcgtcaag gacaaccatg tggacgcggc cgggtccatc gaagaggcgg tacaccgggt
     2641 gcgtgacgcc ttcccggggg ttcctgtcga ggttgaggcc aggaccctcg aggacgtcgg
     2701 gctgctgctg aagctggggg tcgaccgcat catgctcgac aacttctccc ttcaggacac
     2761 gaagagggcg gtggccttgg tctcggggag ggtccccctc gaggcctcgg gcaacatcac
     2821 cctggacagg gtgagggagg tcgccgagac cggcgtcgac ttcatatccg tgggcgagat
     2881 cacccactcg gtgcgcgcgc tcgacatagc catgaccatc gaggtgtctt catgaacgcg
     2941 atcgaaccgg cagccgtgat caggcgggca cggggtcgtt acggccgcag gctcctcatc
     3001 ctcgcgcacc actaccaggc cgacgaggtg gtcgcccacg cggacttcgt gggggactcg
     3061 ctggaactcg caagaagggc ggcccgcgaa aaagaggcgg agttcatcgt gttgtgcggg
     3121 gtgcacttca tggcggagac ggcggccatc ctctcgccgg gcaagaaggt gtacatcccg
     3181 gatccccagg ccggctgccc cctcgccgac agcgc
//
LOCUS       DAOSCR010000305         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19332_length_3203_cov_3.016518, whole genome shotgun sequence.
ACCESSION   DAOSCR010000305 DAOSCR010000000
VERSION     DAOSCR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19332_length_3203_cov_3.016518"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PLT69_07985"
     CDS             <1..1395
                     /locus_tag="PLT69_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14413.1"
                     /translation="PSTDQDAEAQDPMKAAVKEAIKAQEDTLNQIAEKVKAEVQEIAN
                     RTVEKIAEMNPELARQLTPRVSNKNWDTLFSVNLTGDEDIPINKRGSGTRRLVLLNFF
                     RAKAEKEATSSEGKLLRESVTEDVIAEIVSRWTGIPVSRLVEGEKEKLLRLDEILHRR
                     IVGQDEAVRAVADAVIRARSGIKDPKRPIGSFIFLGPTGVGKTELAKALAEALFDTED
                     NMVRIDMSEYMEKHAVSRLIGSPPGYVGYEEGGQLTEAVRRKPYSVVLFDEIEKAHHD
                     VFNVLLQILDDGRVTDSHGRTVNFKNTVIIMTSNIGSTDLIEGVGPTGEISARARESV
                     MSQLRVHFRPEFLNRVDDIVLFKALSLEEIKRIVDLLTSEIEKRLKERRITFLLSDEA
                     KEFIARQGYDPVYGARPLKRFIQRELETRLGRAIIAGEITDGSTVRVDVRESRLEFTT
                     EAESPERVQAVSAV"
     gene            complement(1528..2277)
                     /locus_tag="PLT69_07990"
     CDS             complement(1528..2277)
                     /locus_tag="PLT69_07990"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ14414.1"
                     /translation="MKITEEACFYPDGYVYFDPSTRGYRRDDLWLEDRIHCWFVHPKP
                     HNEQKQLVVHLHGNAENMTSHITGVMFLLEAGFPVLAFDYSGYGQSRGRPTLKGIQED
                     ARLVFSHVLAHPETFGGSVFGFGQSMGAYTLARVLPDFPGLKGAILEAGLHSFHALFT
                     QAYPDFACEVPSEGFSALDTLPLSRVPKLFIHGTADVVVPFTHSIDMHEAASDPKDIL
                     ILDGVGHIDAFISRHSFAYREKIMDFLKRWG"
     gene            2364..3095
                     /locus_tag="PLT69_07995"
     CDS             2364..3095
                     /locus_tag="PLT69_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOJ14415.1"
                     /translation="MAQMVTFLSDFGSKDAYVAQVKAVILSSAPSVTIVDITHEVEPF
                     DLVSAGWLLAASYRHFPRGTVHLAVVDPGVGTARAALVARKDGHTFVGPDNGVFSFIY
                     PADEVIEVTWRPGKAVSNTFHGRDLFAPLVVRLVTGHGLGVPGRPMAEPVRLEVDGPM
                     VVHVDRFGNVITNIPAERLARGGGVEINGTRVSRTVSTFAELQDGSLGLICGSSGTIE
                     VVSHRARASDVLCAGPGTPVSLIGP"
BASE COUNT          609 a          996 c         1075 g          523 t
ORIGIN      
        1 cccagcacgg accaggacgc cgaagcgcaa gacccgatga aggctgccgt gaaggaagcc
       61 atcaaggcac aggaggatac actcaaccag attgcggaga aggtgaaggc agaggtccag
      121 gagatcgcca accggacggt tgagaagatc gcggagatga acccggagtt ggcacggcag
      181 cttacgccca gagtctcaaa caagaactgg gacaccctgt tttcggtcaa cctcaccggc
      241 gacgaggaca ttccgatcaa taagcgtggg agcgggacgc gccggcttgt cttgctgaac
      301 ttctttcgtg caaaggccga gaaggaggcc acctcgtcgg agggcaagct ccttcgcgag
      361 agcgtgaccg aggacgtgat cgccgagatc gtgtcccgat ggacgggcat cccggtctcg
      421 cgcctcgtgg agggcgagaa ggaaaagctc ctgaggctcg acgagatcct ccacaggcgc
      481 atcgtgggcc aggacgaggc ggtccgggcg gtcgccgacg cggtgatccg cgcccgctcg
      541 ggcatcaagg acccgaagcg gcccatcggt tcgttcatct tcctcggacc cacgggcgtg
      601 ggcaagacgg agctcgccaa ggcgctggcc gaggcgctct tcgacaccga ggacaacatg
      661 gtgcgcatag acatgagcga gtacatggaa aagcacgcgg tcagcaggct catcggctcc
      721 ccgcccggct acgtgggtta cgaggaaggc ggccagctca ccgaggccgt acggcgcaag
      781 ccctactccg tggtgctctt cgacgagatc gagaaggccc accatgacgt attcaacgtg
      841 ctcctccaga tcctcgacga cgggagggtc accgactccc acgggaggac cgtgaacttc
      901 aagaacacgg tcatcatcat gacgagcaat atcgggtcaa cggacctcat cgagggcgtc
      961 ggccccacgg gggagatctc ggcccgggcc agggagtcgg tcatgtcgca gctcagggtc
     1021 cacttcaggc cggagttcct gaacagggtg gacgacatcg tgctcttcaa ggcactcagt
     1081 ctggaggaga tcaagcgtat cgtggacctc ctcacctccg agatcgaaaa gaggctgaag
     1141 gagcgacgga taaccttcct cctgagcgat gaggccaagg aattcatcgc ccgccagggc
     1201 tacgacccgg tctacggcgc ccgtccgctc aagcgtttca tccagcggga gctggagacg
     1261 aggctcggca gggccatcat agcgggagag atcaccgacg gcagcacggt acgggtggat
     1321 gtcagggaat cgaggctcga gttcacaacc gaggccgagt cgcccgagcg tgtccaggcg
     1381 gtctcggccg tctgaggcgg ccggtccgag aaaccgcggg gggcctcaag ccccccgcac
     1441 cgaaaacctc ctttttcgca tcctcctctt gagaaggcac ccatacccga gacgcccgcg
     1501 tgcacctgtg ggacacgcag gcggttatca cccccatcgc ttgaggaagt ccatgatctt
     1561 ctcccggtag gcaaacgaat gcctcgagat gaaggcgtct atgtgcccga cgccgtcgag
     1621 gatgagaatg tccttggggt cggacgccgc ctcgtgcatg tctatggagt gcgtgaacgg
     1681 gaccacgacg tcggcggtcc catggatgaa gagcttcggc acccggctga gcggcagggt
     1741 gtcgagggcc gagaagccct cggacggcac ctcgcacgcg aagtccgggt atgcctgggt
     1801 gaaaagcgca tggaaagaat ggagtcccgc ctccaggatc gctcctttga gcccggggaa
     1861 gtccggcagg accctggcga gggtgtacgc ccccatggac tgtccgaacc cgaagaccga
     1921 gccgccgaag gtctcggggt gggccaggac gtgggagaac acgagccttg cgtcctcctg
     1981 tatgcccttg agcgtcggcc tcccccgaga ctggccgtac ccgctgtagt cgaaggcgag
     2041 cacggggaac ccggcctcga ggaggaacat gacgcccgtg atgtgcgagg tcatgttctc
     2101 ggcgttgccg tggaggtgca cgacgagctg tttctgttcg ttgtggggtt tggggtgcac
     2161 gaaccagcag tggattcggt cttcgagcca gaggtcgtcc cgtcggtacc cgcgggtcga
     2221 cgggtcgaag tagacgtaac cgtcggggta gaaacaggcc tcctcggtga tcttcacggc
     2281 cccactataa tgcaggtcca gccttgtccg caatcgtctc ttgcgtgttg acccggggag
     2341 gagttccggt acaatggtcg gacatggcgc agatggtcac cttcctctcg gacttcgggt
     2401 cgaaagacgc ctacgttgcc caggtcaagg cggtgatcct ctcgtcggcg ccctcggtga
     2461 cgatcgtgga catcacccac gaggtggaac ccttcgacct cgtctccgcg ggctggctcc
     2521 tggccgcgag ctacaggcac tttcccaggg gcaccgtgca cctcgcggtc gtcgacccgg
     2581 gcgtggggac cgcaagggcg gcgctcgtgg cgagaaagga cggccatacc ttcgtggggc
     2641 cggacaacgg cgtcttctcg ttcatctacc ctgcggacga ggtcatcgag gtgacctggc
     2701 gtccgggaaa ggccgtgtcg aacacgttcc acgggaggga cctgttcgcg cccctggtgg
     2761 tgaggcttgt caccggccac ggcctggggg tgccgggtcg gccgatggcg gagccggtgc
     2821 ggctcgaggt cgacgggccc atggtcgtcc acgtcgaccg tttcggcaac gtgatcacga
     2881 atatcccggc ggagaggctc gcacggggcg gtggggtgga gatcaacggc acgcgggtat
     2941 ctaggacggt gtccaccttc gcggagcttc aggacggctc cctcggcctc atctgcggga
     3001 gctcgggcac catcgaggtc gtgtcacacc gcgcccgcgc atccgacgtg ctctgcgcgg
     3061 ggcccggcac gcccgtgtcc ctcatcggcc cgtgacacgc cgtctcaggg cgctcctcgc
     3121 ggatccgccg cacgggtcga ccatgccctg cccgaaagga ttccaccgcc aagccggaca
     3181 cgtctgtcat acatccatcg cac
//
LOCUS       DAOSCR010000306         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19449_length_3189_cov_2.962029, whole genome shotgun sequence.
ACCESSION   DAOSCR010000306 DAOSCR010000000
VERSION     DAOSCR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19449_length_3189_cov_2.962029"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLT69_08000"
     CDS             complement(<1..789)
                     /locus_tag="PLT69_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ14416.1"
                     /translation="MPTFTDERFRPDTLLAVACLVVIIAGMKAASGIIVPLLLAVFIS
                     MMCKTPFAWLKKKGVPALPAIVIIMTVITSALFALGSYLGISINDFLQELPVYQSRFQ
                     DLLDGITSRLARYGLNVPHRVVDYFDPSSIVQIFANIIGGLGNMVTWSVLILFMVVFI
                     LMESHTFVGKIKTAFRATPDTLEILDFIFMCVGRYVAIKTLISIATGLSIMLWLTAIG
                     VDYAVLWGLIAFLMHYIPNIGSLVAAIPAILFSVVDAGTTTAILA"
     gene            complement(794..1291)
                     /locus_tag="PLT69_08005"
     CDS             complement(794..1291)
                     /locus_tag="PLT69_08005"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOJ14417.1"
                     /translation="MPRLIRKPPMPIDTIMGRTLTRLLVAAAALLLLFSAPLRLEAQE
                     PTGDLCIVVRGVRNLSGVLNITVADSKGSYLGRERPFALMKTKVTGFETRVVLAGIPR
                     KDYAVYAFHDANGNGALDRNVRGLPKEPSGFSTPHARADQPPPYEKAKFRLDSKEKTV
                     VIDLR"
     gene            complement(1294..1896)
                     /locus_tag="PLT69_08010"
     CDS             complement(1294..1896)
                     /locus_tag="PLT69_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOJ14418.1"
                     /translation="MNEREQKARIMPGMPASILPVHVEGGMRETPYLQDRSDLIEVIR
                     KIPFFSSYTDEQLLEILSLSRIRTFEPGEYITRQGEYDSWLYIVLSGAVNVSSEGTTI
                     AQIRDAGQTIGEMALVDGEPRSASAKAVAKTTTLAIDMSLSERLNESEKERFEAVYYK
                     LLAKILVDRLRKTTHELAQARNNVKELTDAILVEQDRSRR"
     gene            complement(2249..2923)
                     /locus_tag="PLT69_08015"
     CDS             complement(2249..2923)
                     /locus_tag="PLT69_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14419.1"
                     /translation="MKDVILEEIVHAMAARPHAAWLWREPLAASASVDDPLVGSLKEW
                     VHPGHLMPWELLPGARGIVAFFIPFSEQVSMGNAQGRFQSASWARASRETNALIDEVG
                     ERLCGMLRSRGHEAVFVPATHDFDEETLTSRWSHRHVAFTAGLGTLGLNGMLITERGC
                     CGRLGSNERPHETGHTTPCRVLPVQEIGVVRHVRRQVPDRRAHRDGSRQKGMLRQPAR
                     KRTAVP"
     gene            complement(2955..>3189)
                     /locus_tag="PLT69_08020"
     CDS             complement(2955..>3189)
                     /locus_tag="PLT69_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOJ14420.1"
                     /translation="DAYVKDNPAVRVDYIHGEPALETLSAQEDTMGFMLPVISKDLLF
                     ATIAKDGRLPRKAFSMGHADDKRFYLECRKIVP"
BASE COUNT          555 a          934 c         1085 g          615 t
ORIGIN      
        1 tgcgaggatc gctgtggtgg tgccggcgtc cacgaccgag aagaggatgg caggtatggc
       61 ggccacgagc gacccgatgt tgggtatgta gtgcatgagg aaggctatga ggccccagag
      121 cacggcgtaa tccaccccga tggcggtcag ccagagcatg atggaaaggc ccgtggcgat
      181 gctgatcagg gtcttgatgg ccacgtacct gccgacgcac atgaagatga aatcgaggat
      241 ctccagggtg tccggggtgg ccctgaaggc ggtctttatc ttgcccacga aggtgtggga
      301 ttccatgagg atgaacacca ccatgaacag gatgaggacc gaccaggtaa ccatgttgcc
      361 gagcccgcct atgatattgg cgaagatctg cacgatcgaa gagggatcga agtagtccac
      421 cacgcggtga gggacgttga ggccgtacct ggccagtcgc gaggtgatcc cgtcgaggag
      481 gtcctggaat cgtgactggt agaccggtag ttcctggagg aagtcgttga tcgatattcc
      541 caggtatgag cccagggcaa agagcgcgga cgtgatgacc gtcatgatga tgacgatcgc
      601 cgggagagcc gggaccccct tcttcttcag ccaggcaaag ggcgtcttgc acatcatgga
      661 gatgaagacc gcgagcagca ggggcacgat gatgccggaa gcggccttca ttcccgcaat
      721 gatgaccacg aggcatgcga cggcaaggag ggtgtcgggc ctgaagcgtt cgtccgtgaa
      781 agtcggcatg gctctacctg aggtcgatca ccacggtctt ttccttggaa tcgagcctga
      841 acttcgcctt ctcgtagggg ggcggctggt cggcccgtgc gtgaggggtc gagaaaccgg
      901 agggttcctt ggggaggccc cgcacgttcc tgtcgagcgc cccgttgccg tttgcgtcgt
      961 ggaaagcgta cacggcgtag tccttccggg ggatgcccgc gaggacgacc ctcgtctcga
     1021 atcccgtgac cttggtcttc atgagggcga acggccgttc acggccgagg taggagccct
     1081 tcgagtccgc gacggtgatg ttgaggaccc cggagaggtt ccgcaccccc ctgaccacga
     1141 tgcagaggtc ccctgtgggt tcctgcgcct cgagcctaag cggtgccgag aagagcaaga
     1201 ggagagcggc ggccgctacg aggagccttg taagggttcg tcccattatt gtgtctatcg
     1261 gcatcggcgg cttgcgtatc aagcggggca cggtcacctc ctcgagcggt cctgctccac
     1321 gaggatggcg tccgtcagtt ccttcacgtt gttcctcgcc tgtgccagct cgtgggtggt
     1381 cttgcgcagc cggtccacga ggatcttcgc gaggagcttg tagtacaccg cctcgaagcg
     1441 ctccttctcc gactcgttca gcctctcgga gagggacatg tctatggcga gggtggtggt
     1501 cttggccacg gcctttgccg atgcggagcg tggttcgccg tccacgagcg ccatctcgcc
     1561 gatggtctgc ccggcatccc tgatctgggc gatcgtggtg ccttcgctcg aaacattcac
     1621 cgcgcccgac aggacgatgt acagccacga gtcgtactcg ccctgcctcg tgatgtactc
     1681 gccgggttcg aacgtcctga tcctgctcag ggagaggatc tccaagagct gttcgtcggt
     1741 ataggaagag aagaagggta tcttgcggat cacctcgatg aggtcggatc gatcctgcag
     1801 gtagggcgtc tccctcattc ccccctccac gtgcacaggc aggatcgagg cgggcatgcc
     1861 cggcatgatc cgtgccttct gctcacgttc gttcatagca ggagggtcgc cctgagtcaa
     1921 agagttcatg ccggtgagtg gggtcgggtc gtgcaaggca aggtgcaggc aggccggtgc
     1981 gctcgtggtg cccgggtctg tcggtagagg cagccgcctc gtgaggagat gcaccccgtc
     2041 cacgtcgtgg gtccggcggc taacgcggtt gtgaagctgc ggaggcccgg tctcttctgg
     2101 gctgtcgtgt tcgtggtgta caggcccatt ccctgtttgt cggagagacc cgggcgccgg
     2161 gcgtgctcga tgcgcccgtg gtcaggggat gcggcatgaa cagggcagcc ccgagacgca
     2221 cttgccgcac gcgtcggccc atccgatgtc agggtacagc ggtgcgtttg cgagcaggtt
     2281 ggcgtagcat tcctttttgt cgagacccgt cccggtgagc gcgccggtcg ggcacctgtc
     2341 gacgcacatg ccgcacgacc ccgatctctt gaacaggcag aactcgacac ggtgtcgtgt
     2401 gtccggtttc atgcgggcgc tcgttggacc cgagcctgcc gcagcagccc ctttccgtga
     2461 tgagcatgcc gttgaggccc agggtgccga gcccggccgt gaaggccacg tggcggtgag
     2521 accacctcga cgtgagggtc tcctcgtcga agtcgtgggt tgcaggcaca aagacagcct
     2581 cgtgacctcg ggaccggagc atcccgcaga gcctttcgcc gacctcgtcg atgagggcgt
     2641 tcgtctctct ggaggcccgg gcccacgatg cgctctggaa gcggccttga gcgttgccca
     2701 tcgaaacctg ctcggaaaac gggatgaaga aagcgacgat gccgcgagcg ccgggcagga
     2761 gctcccaggg catgaggtgg ccggggtgca cccactcctt gagggacccg accagggggt
     2821 cgtccaccga ggccgaggcg gcgagcggct ctctccacag ccaggccgca tgggggcgtg
     2881 cggccattgc gtgcacgatc tcctcgagga tgacgtcttt cacgcggccg cctcgagatg
     2941 caccgggcgc aagctcatgg aacgatcttc ctgcattcca ggtagaacct cttgtcgtcg
     3001 gcgtgcccca tggagaacgc ctttcggggg agcctcccgt ctttggcaat ggttgcgaac
     3061 agcaagtcct tcgagatcac gggcagcatg aatcccatgg tgtcttcctg ggccgagagc
     3121 gtctccagcg caggttcgcc gtggatgtag tcgaccctta cggcggggtt gtccttcacg
     3181 tacgcgtcg
//
LOCUS       DAOSCR010000307         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19464_length_3187_cov_2.884738, whole genome shotgun sequence.
ACCESSION   DAOSCR010000307 DAOSCR010000000
VERSION     DAOSCR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19464_length_3187_cov_2.884738"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLT69_08025"
     CDS             <1..1178
                     /locus_tag="PLT69_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ14421.1"
                     /translation="AAYDETITIDLSVLEPLAACPHSPDAVMPVKDIPRTKVDQVIIG
                     SCTNSSMKDMLTVAALLKGRKVHPDVSLAIAPGSRQVLEMLAVTGALGDIISAGARIL
                     ETACGPCIGMGQAPASGSVSVRTFNRNFKGRSGTSDAMVYLASPETACACALTGVITD
                     PRELGAYPKIEQPASFTVDDSMILRPAQDPSRVQIRRGPNIRPLPKRAPMEDSFVLKV
                     LLRLQDNVTTDDIMPAGSKILPLRSNIEAISRYVFSPIDDTFSERAKASGAGCIVAGA
                     NYGQGSSREHAALAPMYLGVKAVFARSFARIHRSNLINFGILPVLIDEAGFERLAAGA
                     TVKVQGIHAALEGRSPLGVTVAETGEVIQAHLEVSAREAQLLKAGGLLNYISHSVRG"
     gene            1180..2580
                     /gene="gltX"
                     /locus_tag="PLT69_08030"
     CDS             1180..2580
                     /gene="gltX"
                     /locus_tag="PLT69_08030"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ14422.1"
                     /translation="MDREIRTRFAPSPTGALHIGGVRTALFSWLHARHHQGKFILRIE
                     DTDAERSTEENTRQILEAMEWLGLDWDEGPYFQAQRLDVYRSYLERLLNAGAAYWCDC
                     EPEDLERKRKQALAEGRKPKYDGTCRDRGLKEGPGRVARFRMPDTGVTAFEDLIKGRI
                     SIDNAEMDDLVLLRSDGMPTYNFTVVVDDASMGMTHVIRGDDHVNNTPRQIRIYEALG
                     LDIPFFAHVPMILGPDKTKLSKRHGATSVMAYKEMGYLPEALVNYLVRLSWSHGDQEI
                     FSRQELIEYFDIQDVGRAAAVFNPDKLLWLNHHYIKTGDPRRLAGLLKEFAARKGYEI
                     PAEDYLVRVVTDVRERTKTLVEMVEFGDFYFRDAAPPEDLMRKFLTPEALEILKALAE
                     EFSRMESMEKEAAEPRVKALLERLGVPFKVVAQPLRVALTGKTVSPGIFEVMATLGKE
                     RVLARLGRVLSTGGPV"
     gene            2637..>3187
                     /locus_tag="PLT69_08035"
     CDS             2637..>3187
                     /locus_tag="PLT69_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ14423.1"
                     /translation="MEMNVRQLAMVSLFMALVTVATMIVRVPIPQTTGYMNLGDSVVL
                     ISGVFFGPALGFIAGGVGSALADILGGYPQWAPWTLLIKGLEAMFAGAAVRRLRLDTS
                     RTTVPMVACFVLCTAWMVLGYYVTEVIMYDQKAALAEVLGEHEGRYVLTLGGRTYPIE
                     ESPPGPPLPAPGGAAGLRRCAERA"
BASE COUNT          589 a         1046 c         1051 g          501 t
ORIGIN      
        1 gcgccgcata cgacgagacc ataaccatcg acctctccgt gctcgagccg ctggccgcgt
       61 gcccgcacag ccccgatgcc gtgatgccgg tgaaggacat cccccgcacg aaggtggacc
      121 aggtgatcat aggctcgtgc acgaactcgt cgatgaagga catgctcacc gtggccgcac
      181 tgctcaaggg caggaaggtc caccccgacg tcagcctcgc catcgcaccg gggtcgagac
      241 aggtgctcga gatgctcgcc gtcacgggcg cgctcgggga catcatatcc gcgggagcga
      301 ggatcctcga gaccgcgtgc gggccgtgca tcggcatggg acaggcccct gcctcgggct
      361 cggtctcggt gcgcaccttc aacaggaatt tcaaggggcg ctcgggcacg agcgacgcca
      421 tggtctacct tgcatctccc gagacggcgt gcgcctgcgc cctcaccggc gtgatcacgg
      481 accccaggga actgggcgca taccctaaga tcgagcagcc cgcatcgttc accgtcgacg
      541 actcgatgat cttgaggcct gcgcaggacc cttcacgcgt gcagataaga cgcggcccca
      601 acataaggcc gttgcccaag cgcgccccca tggaggacag cttcgttctc aaggtcctcc
      661 tcaggctcca ggacaacgtg accaccgacg acatcatgcc ggccgggtcc aagatcctgc
      721 ccctcaggtc caacatcgag gccatatcga ggtacgtctt ttcccccatc gacgacacgt
      781 tctccgagag ggcgaaggca tcgggcgcag ggtgcatcgt ggccggggcc aactacggcc
      841 agggctcgtc cagggaacac gcggccctcg cccccatgta cctcggcgtc aaggccgtgt
      901 tcgccaggtc tttcgcccgc atccaccggt cgaacctcat caatttcggg atcctgcccg
      961 tcctcatcga cgaggccggg ttcgagcgcc tcgccgcagg cgcgaccgtc aaggttcagg
     1021 gcatccacgc ggcgctggag gggagatcgc cgctcggcgt cacggtcgcc gagacgggcg
     1081 aggtcatcca ggcgcatctc gaggtctcgg cccgggaggc acaactcctg aaggcagggg
     1141 gcctgctcaa ctacatctcg cattcggtcc gggggtgaca tggacagaga gatcaggacg
     1201 cgcttcgcac catcgccgac gggggcgctc cacatcggcg gcgtcaggac ggcgctcttc
     1261 tcgtggctcc acgcgaggca ccaccaaggc aagttcatcc tgcgcatcga ggacaccgac
     1321 gcagagcggt ccacggaaga gaacacacgc cagatcctcg aggcgatgga atggctcggc
     1381 ctcgactggg acgaaggccc gtacttccag gctcagcggc tcgacgtcta caggtcgtac
     1441 ctcgagcgcc tcctgaacgc gggcgcggcc tactggtgcg actgcgagcc cgaggacctc
     1501 gagaggaaac gaaagcaggc cctggccgag gggagaaagc cgaagtacga cgggacgtgc
     1561 agggacaggg ggctcaagga ggggcccggc cgcgtggcga ggttccgcat gcccgatacg
     1621 ggcgtcaccg ccttcgagga cctcatcaag gggaggatct ccatagacaa cgccgagatg
     1681 gacgacctcg tcctcttgcg ctcggacggg atgcccacgt acaacttcac cgtggtggtg
     1741 gacgatgcgt ccatgggcat gacgcacgtc atcaggggcg acgaccacgt gaacaacacg
     1801 ccgaggcaga tccgcatata cgaggcactg ggcctggaca tccccttttt cgcccacgtc
     1861 cccatgatcc tgggccccga caagaccaag ctctccaaga ggcacggcgc cacctcggtc
     1921 atggcgtaca aggagatggg ctacctgccc gaggccctcg tgaactacct cgtgaggctc
     1981 tcatggtccc acggggacca ggagatcttc agccggcagg agctcatcga gtacttcgac
     2041 atccaggacg tggggagggc tgcggcggtc ttcaaccccg acaagctcct gtggctcaac
     2101 caccactaca tcaagaccgg tgacccgagg cggctggcgg gcctgctgaa ggaattcgcg
     2161 gcccgcaagg gctacgagat cccggcggaa gactacctgg taagggtggt gaccgacgtg
     2221 agggagcgta ccaagacgct cgtggagatg gtggagttcg gcgacttcta cttcagggac
     2281 gcggctccgc cggaagatct catgcggaag ttcctcaccc cggaggccct ggagatcctc
     2341 aaagcccttg ccgaagagtt ctcgcgcatg gagagcatgg agaaggaggc ggccgagcct
     2401 cgcgtgaagg ccctgctcga gaggctgggg gtgcccttca aggtcgtagc gcagccgctg
     2461 agggtggcgc tcacgggcaa gaccgtgtcc ccggggatct tcgaggtcat ggcgaccctc
     2521 gggaaggaac gcgtcctggc caggctcggc agggtcctct ccacgggagg ccccgtctga
     2581 gaggatgcag atgacgttga atgtgcgtgc agcactgtca tgacagggag ggcggcatgg
     2641 agatgaacgt gagacagctc gccatggttt ccctcttcat ggccctggtg acggtcgcca
     2701 ccatgatcgt cagggtcccg atcccccaga ccacagggta catgaatctg ggcgacagcg
     2761 tggtgctcat tagcggcgtc tttttcgggc ccgccctcgg cttcatcgcc gggggcgtgg
     2821 gatcggccct cgcggacatc ctgggcggct atccccagtg ggcgccgtgg acgctcctca
     2881 tcaagggcct ggaggccatg ttcgcgggtg ccgcggtcag gcgcctgcgc ctggatacga
     2941 gcaggaccac ggtgccgatg gtggcctgtt tcgtcttgtg caccgcatgg atggtcctcg
     3001 ggtactacgt gaccgaggtg atcatgtacg accagaaggc cgccctcgcc gaggtgctcg
     3061 gtgagcacga gggccgctac gtcctcacgc tcggcgggcg cacctatccc atcgaggaga
     3121 gcccccccgg gcctccgctc cctgcgcccg ggggcgcagc cggcctacgt cgatgtgcag
     3181 agcgcgc
//
LOCUS       DAOSCR010000308         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19469_length_3186_cov_4.045992, whole genome shotgun sequence.
ACCESSION   DAOSCR010000308 DAOSCR010000000
VERSION     DAOSCR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19469_length_3186_cov_4.045992"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="argC"
                     /locus_tag="PLT69_08040"
     CDS             complement(<1..821)
                     /gene="argC"
                     /locus_tag="PLT69_08040"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOJ14424.1"
                     /translation="MSLSVGILGATGYTGAELLRILYLHPQARIVYCSSRQYNGKALA
                     DVLTFFRGSLDIPLEEFDEETVRNRCDIVFSCLPHGASMEFVAGIAPFVKVVDLSADF
                     RLRDVSVYRAWYQEHSCPELLGKAVYGLCEIYRDLIRHAGLVANPGCYPTSVLLPLVP
                     LLREGLIDKDGIVVDSKSGVSGAGRGLSLRSHICEVGESFSAYKVGRSHRHIPEIEQE
                     LAMAAGAPVTIEFTPHLVPMSRGMLSTIYVGTTADEAELKGCLERFYLGSPFVSVV"
     gene            complement(829..1221)
                     /gene="rpsI"
                     /locus_tag="PLT69_08045"
     CDS             complement(829..1221)
                     /gene="rpsI"
                     /locus_tag="PLT69_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ14425.1"
                     /translation="MAEKSYATGRRKNATARVWLSLGKGEMIVNGKPVDEYFTRKTLE
                     LNIRKPFEITDTVDQFDVYATVSGGGISGQAEAIRHGISRALAAFDIDRFRPKLKAAG
                     FLTRDPRMVERKKYGLHKARKRPQFSKR"
     gene            complement(1233..1661)
                     /gene="rplM"
                     /locus_tag="PLT69_08050"
     CDS             complement(1233..1661)
                     /gene="rplM"
                     /locus_tag="PLT69_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOJ14426.1"
                     /translation="MGTFQAKKGEIEREWVLIDAKDAVLGKLAVKAASILRGKTKPEF
                     TMHSDVGDFVIIINADKVRLTGNKQDQKRYYRHSGYMGGLKSTTARELLQKKPEDVVK
                     HAVRGMLPKNNLGRAMFKKLKVYAGAEHPHEAQKPRQITL"
     gene            1823..2773
                     /locus_tag="PLT69_08055"
     CDS             1823..2773
                     /locus_tag="PLT69_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ14427.1"
                     /translation="MVIDAVVVAGDGKAARTVFKKNKALLDVGGRPILRHIVETLKSC
                     RAIRRIVVVGPKDAFDPIIGDTGVLVVPQKRSLAENGWEGFLHTLPGYASKLDISAEL
                     VERNFHKQVLFLSGDIPLLCKEEIEEFLSGCDFERYDYLAGITSEESLEAFGPRKGKP
                     GIKMATFHTRDGNFRQNNLHVARPFVLLNSIDLVLKAYEYRYQKKLGNILRALVEILR
                     LGPGKVGATLLLYLVLQLSALFGALGMNRAARWASYPVTRNQIERVVSSLLDARFKLV
                     ETTIGGAALDVDNEKDYMTLSVMYRDWVNLIRQRAHQRAK"
     gene            complement(2755..>3186)
                     /locus_tag="PLT69_08060"
     CDS             complement(2755..>3186)
                     /locus_tag="PLT69_08060"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOJ14428.1"
                     /translation="SLALQDWTCGYMRRETCWNWASTAWTLPDGRSYGMNLSCGVNET
                     SFTENAFWIDGAMTKVDTVDFVYDPDDVMAPWEISSSDGRVRLSFLPEGKRGEKVNAF
                     FVASRFTQLVGTFSGTVTSAQGETIDITGCPGFAEDHFARW"
BASE COUNT          568 a          982 c          987 g          649 t
ORIGIN      
        1 acgacactga cgaacggcga ccccaggtag aaacgctcga ggcagccctt cagctcggct
       61 tcgtccgccg tcgtgcccac gtagatcgtg gagagcatcc ccctgctcat gggcacgagg
      121 tgcggggtga actcgatggt caccggggcg cccgcggcca tggccagctc ctgctctatc
      181 tcggggatgt ggcggtgtga gcggcccacc ttgtaggcgg agaacgattc gccgacctcg
      241 cagatgtgcg acctgaggct caggcccctg ccggcgccgg acaccccgct cttgctgtcg
      301 accacgatgc cgtccttgtc tatgagacct tcccgcaaga ggggcaccag gggcaaaagg
      361 acgctcgtgg ggtagcaacc cgggttcgcc accagacctg cgtgcctgat gagatcccgg
      421 tatatctcgc agaggccgta gacggccttt ccgaggagct cgggacagga atgctcctgg
      481 taccatgccc ggtagaccga cacgtctctc aacctgaaat cggcgctcag atccacgacc
      541 ttcacgaacg gcgcgatccc ggccacgaac tccatgctcg cgccgtgagg caggcagctg
      601 aacacgatgt cgcagcggtt ccttaccgtt tcctcgtcga actcctcgag agggatgtcg
      661 agagagcccc tgaagaaggt gaggacgtcc gcaagggctt tcccgttgta ctgcctcgac
      721 gagcagtaga cgatccttgc ctgcgggtgc aggtaaagga tcctcaggag ttccgcaccg
      781 gtatatcccg ttgcccccag gattccgacg gaaagagaca aggaagcgct acctcttgga
      841 gaactgcggt cttttgcgcg ccttgtggag gccgtacttc ttgcgttcca ccatgcgggg
      901 atcgcgggtg aggaaacccg cggccttgag cttgggacgg aacctgtcta tgtcgaacgc
      961 cgcgagcgcc cgcgagatcc cgtggcggat ggcttccgcc tggccggaga tccctcctcc
     1021 gctcacggtg gcgtagacgt cgaactggtc gacggtgtcc gtgatctcga agggtttcct
     1081 gatgttgagt tccagcgtct tcctggtgaa atactcgtcc acgggcttcc cgttgacgat
     1141 catctcgccc ttgccgagag agagccagac cctggctgtt gcgttcttcc tgcggcctgt
     1201 tgcgtaggac ttctctgcca tggcgacccc cctcagagcg tgatctgccg aggcttctgc
     1261 gcctcgtgcg ggtgttcggc gcccgcgtag accttgagct tcttgaacat ggccctgccg
     1321 aggttgttct tggggagcat gcccctcacc gcgtgcttca ccacgtcttc cggcttcttc
     1381 tggaggagct ctcgggccgt ggtgctcttg agccctccca tgtacccgct gtgccggtag
     1441 taccgcttct ggtcctgctt gttgccggtg agcctgacct tgtcggcgtt gatgatgatg
     1501 acgaagtcac cgacatcgct gtgcatcgtg aactccggct tcgtctttcc cctgaggatg
     1561 gatgccgctt tgaccgcgag ctttccgagc accgcgtcct tcgcgtctat gagcacccac
     1621 tccctttcta tctcgccctt cttcgcctga aaggttccca ttgaacgcaa ctccttgggt
     1681 cgtcaaatag ggctcggata tatccgaaga ctttctcgat gtcaagggcc gatgctgacg
     1741 accgtgaaga ccgggcgaag ggcgtaccgg agaagacggt atcgcttgac atggttgagt
     1801 gttttcagtt cattgtgttc gtatggtcat agatgcggtc gtggtcgcgg gggacggcaa
     1861 ggccgcccgc acggtcttca agaagaacaa ggccctcctg gatgtgggcg ggaggcccat
     1921 tctccgtcac atcgtggaga ccttgaagtc gtgccgcgcc attaggcgca tcgtcgtggt
     1981 cgggccgaag gatgcgttcg acccgatcat cggcgacacg ggggtcttgg tggtccccca
     2041 gaagaggagc ctcgcagaga acgggtggga ggggttcctc cacaccttgc caggctatgc
     2101 ttccaagctc gatatcagcg ccgagctcgt ggagagaaac ttccacaaac aggtcctctt
     2161 cctttcaggg gacattcccc tcctctgcaa ggaggagata gaggagttcc tctcagggtg
     2221 cgacttcgaa cggtacgact acctggcggg catcacgtcg gaagagagcc tggaggcgtt
     2281 cgggccacgc aagggcaaac cgggcatcaa gatggcgacc ttccacacgc gtgacgggaa
     2341 cttcaggcag aacaacctgc acgtggcaag gccgttcgtt ctcctcaaca gcatagacct
     2401 cgtactcaag gcctacgagt acaggtatca gaaaaagctc ggcaacatcc tgcgcgccct
     2461 cgtggagatc ctgcgcctcg gcccggggaa ggtgggggcc acgctgctgt tgtacctggt
     2521 gctccagctg tcggcgctct ttggcgccct cgggatgaac cgggccgccc ggtgggcgag
     2581 ttaccccgtc acgaggaatc agattgaaag ggtcgtctcc tccctcctcg acgccaggtt
     2641 caagctcgtg gagacgacca tcggcggtgc ggccctcgat gtagacaacg agaaggatta
     2701 catgacgctc tcggtcatgt accgggactg ggtgaacctc ataaggcaga gggctcacca
     2761 gcgggcgaag tgatcctcgg cgaacccggg gcagcccgtt atgtctatcg tttccccttg
     2821 agcgctcgtg acggttccag agaacgtccc cacgagctgc gtgaaccgtg aggcgacgaa
     2881 gaaggcgttg accttctccc ccctcttgcc ttcggggagg aacgagaggc gtaccctgcc
     2941 gtcggacgag ctgatttccc agggggccat gacgtcgtcg gggtcgtaca cgaaatcgac
     3001 cgtgtcgacc ttggtcatgg cgccgtctat ccagaaggcg ttctcggtga agctcgtctc
     3061 gttgaccccg catgagaggt tcatgccgta gctccgcccg tcgggcaggg tccacgcggt
     3121 ggaagcccag ttccagcacg tctccctcct catgtagccg cacgtccagt cctggagcgc
     3181 gaggct
//
LOCUS       DAOSCR010000309         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19479_length_3185_cov_3.357508, whole genome shotgun sequence.
ACCESSION   DAOSCR010000309 DAOSCR010000000
VERSION     DAOSCR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19479_length_3185_cov_3.357508"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..312)
                     /locus_tag="PLT69_08065"
     CDS             complement(73..312)
                     /locus_tag="PLT69_08065"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ14429.1"
                     /translation="MTKARREGVPGKLLRRLARLSALSFILVVMTFLGLYAGMYLDKL
                     THMAPNFTFLGLVLGIALGFRGFVQEVLVERRKES"
     gene            complement(353..529)
                     /locus_tag="PLT69_08070"
     CDS             complement(353..529)
                     /locus_tag="PLT69_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14430.1"
                     /translation="MVALWIVIGCLFLTGIGIRFMYRVLGLTPAEATAVFVLIVTLVG
                     VNSAPARQLIAQLF"
     gene            1545..2708
                     /locus_tag="PLT69_08075"
     CDS             1545..2708
                     /locus_tag="PLT69_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ14431.1"
                     /translation="MGEELVYLDNAASSWPKPEPVVRAVERMVREVGANPGRSGHRLS
                     VEAARAITEARCAMAGLLGASNPLRVVFTKNATEALNMAIRGLLRPGDRVVVSSMEHN
                     SVMRPLMAERGRGVEVEVVRCSPEGRLDPDDVKRALKRRTRLVIHVHASNVSGCVTPA
                     AEIGRIAREHGAFYCVDAAQSAGCLPIDVERMNVDLLAFTGHKSLYGPQGTGGLYVAE
                     GVDAHLQPLMAGGTGSLSEREEQPAFMPDRYESGTPNTPGIAGLLEGVTFVASLGVDT
                     IRRHETSLAALLMDALSEIPGVRVVGPTDRESRVAIVSIVVAGMDPGEIAYRLDEDHG
                     VLCRPGLHCAPSAHATLATFPAGTLRFSIGYFNTEEHIHRAVSALKSIVAKGG"
     gene            2711..3172
                     /gene="yedF"
                     /locus_tag="PLT69_08080"
     CDS             2711..3172
                     /gene="yedF"
                     /locus_tag="PLT69_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOJ14432.1"
                     /translation="MGFIVDAKNLACPQPVVLTKKALEDHDEVVVLVNDPTARSNVKK
                     LAQSLGCTVAEEETGECTRITISRAARCDALPETAQNDGGPLVLALSSETMGSGDEEL
                     GRLLMKGFLHTIVEAESKPDVVVLFNAAVKLAVEGSEVLDDLKDLAFHLHR"
BASE COUNT          589 a          945 c         1107 g          544 t
ORIGIN      
        1 aggtgttctt ggtcaagaaa cggtgggtga tggccgcgaa gaaaaccagc ccaatggcga
       61 cgaagaccag tgtcatgatt cctttctcct ttcaacgagc acttcctgga cgaaccccct
      121 gaacccgagc gcgataccca gcacgagacc gaggaaggtg aagttcggcg ccatgtgggt
      181 cagcttgtcg aggtacatgc ccgcgtacag accgaggaag gtcatgacca ccaggatgaa
      241 gctcagggcg ctcagcctcg cgagtctcct caagagcttt ccaggaaccc cctcgcgcct
      301 tgccttggtc atgtgccggg cctcccgtgc gtgcttcttg aacgacggcg tcctagaaga
      361 gctgtgcgat gagctgacgc gccggggccg agttcacgcc gacgagcgtg acgatgagca
      421 cgaacacggc ggttgcctct gccggggtca gcccgagcac cctgtacatg aacctgatcc
      481 cgatgccggt gaggaacaag caacctatca cgatccagag agcgaccatc tcgttatctc
      541 cttcgttgtc ttgatgaccc tggtaaggca aggcccatgc caactgaacc catacataac
      601 cttttgaaat aactggagga taaaccagga cctcatcccg gggttgtcat tttgtcagca
      661 aggggcccgt ggagggcagc gagagtgaca aagtgacaaa aagcaccctc gcagcgcctg
      721 acgcgtgaca ttctgtcacc tgtcccgtat ggaacggttg tctgaaggcc ttcgcgggtg
      781 gacaacgaac gcccggccca agacgctcga tgagttcggg cccgcatgag gcagggcgac
      841 tcgggcggac cccaggggga agacatcgtc gaccgcctgg gtctttgtct cccctggcct
      901 gggccgaggc atgcggggtg gtccatcggg cggctgcagc atggaggccc gcgcaccgaa
      961 tcggctgcac gttgtcgtgt gcggggttta agtaagccgt actctgcccg tgtgcggagg
     1021 cgggccgggt caagggcatc gcaccttcgg gcacaggctg gatgcttggg cgtcgccctg
     1081 tggccgggat gccgtcaccc tggctccggc tcgttcacac ggggtgagga tgctgccggc
     1141 gcggatcgcg tacgcgccgg tctccttcgg ctcgtttcca cgggggggga ggatgggtca
     1201 gcactgatgc agaggggaaa ggtgcgtgac tcgagctcga tcgtcagcag ggccccctct
     1261 tcctgcttcg atgtcgtttc cgggcaaggt gtgggcgtgg ggccaggtga cggggcaggg
     1321 tgcggggccc cggccgttcc ggcgtcaggg ctctaggtgt tggtcatgaa aggcgggaat
     1381 gcaatggggt cgtcgatgca aacatggaca ttgacacggg agatcaacgt gcaccatgct
     1441 ggcctcgatc tcatggagga tgcagcgaaa gatggctgtc tcacgaggcg gattggaccg
     1501 caggcatggt gaaccggagc gggtgaggat ccgggggctc gggtatgggt gaggagctcg
     1561 tctacctcga caacgcggcg agctcctggc ccaagccgga gccggtcgtg cgggccgtgg
     1621 agcgcatggt gcgggaggtg ggcgcgaacc cggggaggtc gggacaccgc ctgtcggtcg
     1681 aggccgcccg ggccataacc gaggcacgct gcgccatggc cgggctgctc ggcgcatcga
     1741 acccgctgag ggtcgtgttc accaagaacg ccaccgaggc cttgaacatg gcgatcaggg
     1801 ggctcttgag gccgggagac agggtggtcg tctcgagcat ggagcacaac tcggtgatga
     1861 ggcccctcat ggccgagagg gggagggggg tcgaggtgga ggtggtgcgg tgctcgcccg
     1921 agggtcgcct cgacccggac gacgtgaaga gggcgctcaa gcgaaggacg aggctcgtga
     1981 tccatgtgca cgcatccaat gtctcagggt gcgtgacgcc cgcggccgag atcgggcgta
     2041 tcgcgaggga acacggagcg ttctactgcg tggacgccgc tcagtccgcc gggtgcctgc
     2101 ccatcgacgt ggagcggatg aacgtcgacc tcctcgcctt taccggccac aagtcgctct
     2161 acgggcccca gggtaccgga ggcctgtacg tggccgaggg cgtggatgcc caccttcaac
     2221 cgctcatggc gggagggacg ggaagcctgt cggagaggga ggaacagccc gccttcatgc
     2281 cggacaggta cgagtcgggg acgcccaaca cccctggtat cgcgggcctc ctcgaaggcg
     2341 tcaccttcgt ggcctccctc ggggtcgata ccatacgcag acatgagacc tcgcttgccg
     2401 ccctgctcat ggacgctctc agcgagatac cgggggtgag ggtggtcggg ccgacggaca
     2461 gggaaagcag ggtggccatc gtctccatcg tggtcgccgg catggacccg ggtgagatag
     2521 cctacaggct cgacgaggac cacggggtgt tgtgcaggcc cgggctgcac tgcgctccct
     2581 cggctcacgc gacgctcgct accttccccg cgggaacgct caggttcagc atcggttatt
     2641 tcaacaccga ggaacacatc cacagggccg tctcggcctt gaaatccatc gtcgccaaag
     2701 gagggtgaac atgggattca tcgtggacgc caagaacctc gcttgtcctc agcccgtggt
     2761 gttgacgaag aaggcgctcg aggatcacga cgaggtcgtg gtcctcgtca acgaccccac
     2821 ggcgagatcg aacgtgaaga aactcgccca gtcgctcggg tgcaccgtgg ccgaggagga
     2881 gacgggagag tgtacccgga taaccatctc ccgtgccgcc cggtgcgacg ccctgcccga
     2941 gacggcccag aacgacgggg gaccgctggt gctggccctc tcctcggaga ccatgggatc
     3001 gggcgacgaa gagctcggga ggctgctcat gaagggcttc ctgcacacga tcgtcgaggc
     3061 ggagtccaag cccgatgtcg tggtcctgtt caacgccgcc gtgaagctcg cggtggaagg
     3121 gtccgaggtg ctcgatgacc tgaaggacct tgcattccat ttgcaccggt gattccttta
     3181 cccgc
//
LOCUS       DAOSCR010000310         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19548_length_3178_cov_2.992315, whole genome shotgun sequence.
ACCESSION   DAOSCR010000310 DAOSCR010000000
VERSION     DAOSCR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19548_length_3178_cov_2.992315"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1501)
                     /gene="amrB"
                     /locus_tag="PLT69_08085"
     CDS             complement(311..1501)
                     /gene="amrB"
                     /locus_tag="PLT69_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOJ14433.1"
                     /translation="MVVFMDPLRMADEGFAVDRRALSVLAMMDGTRDLLDIQAELMRV
                     SGGSIVPMEHIEAFLERLDEALLLDSRRFRDRRRAVEEEFLRAGIRRTILDGRSYEAD
                     PEALAASIEELERSLPPLPAGTKDGISGVVAPHIDLKVAFRTYVDVYRRLRDASFDTV
                     VVLGVNHYGSDGLYCLTEKDFETPFGVLKTHREIVRRLSRGMPPGGVAPTDLDHKSEH
                     SIEFQALFIAYYLGVSRTRIVPVLCGSFHEFVRSGRDPAEDPRFIAFRDALCEVVSAD
                     PGRVLVVAGVDFSHVGLKFGHDQRADRLLDKAMEHDRALLGCLMEGDASGLFGRAASC
                     RDAYNVCGLPALVLMSSVLGKRGCELVAHEVYDERVTGSAVTYAALAFSGARDTAQRV
                     GESG"
     gene            complement(1682..1819)
                     /locus_tag="PLT69_08090"
     CDS             complement(1682..1819)
                     /locus_tag="PLT69_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOJ14434.1"
                     /translation="MGKGDQRSKRGKIFRGTFGKTRPKRKKAPEAPAARGAKTARKAA
                     K"
     gene            complement(2163..3038)
                     /gene="hslO"
                     /locus_tag="PLT69_08095"
     CDS             complement(2163..3038)
                     /gene="hslO"
                     /locus_tag="PLT69_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOJ14435.1"
                     /translation="MEDYLVRGMTEGGNVIGLACVTTNLVEDARRRHKTCPTATAALG
                     RALTGGGLLGALLDEGQRVAIKFTGDGPLKKIVVEAEGDGTVRGYVQVPRVDLPPVDG
                     KLDVGGAIGRRGFLTVTRDLRIRAPYSGIVELHTGEIASDIAYYLSESEQVPSAVGLG
                     VYVEADERVGASGGFLVQSLPPRDDALISSIERTIQSLPGVTTLLMQGKTPEDILGMI
                     FTQTPFRIIETRRVSFACSCSVGRIEQALVTLGKRQIEEIAATEEMFDIFCHFCNKTY
                     VFTREHLLRLAREMH"
BASE COUNT          540 a         1087 c          986 g          565 t
ORIGIN      
        1 ggagtagttt ttcaggtacc acagcacgta ggtgagggtg acgctgtcct tccccccgga
       61 gagcgcaacg cagaccttgt ccccctcgac gatgagcccg taacgctcga gggcatggat
      121 cacgtcggtc aggaaccact tgccgaacaa ggggttgtgt cccatgggga tgtccatcgt
      181 ctcgtgacct cctctcgata tcgacctcga aggcggcaga ccgctgcaag gaaggccttc
      241 gtttcatgga atgcacgagg ccctcgcctg gcgggtgcgc ccccaagggg cgtccggggg
      301 cctgcgtgcg tcaaccgctc tccccgacgc gctgagccgt gtcccgtgcg ccggaaaagg
      361 cgagggcggc atacgtcacc gcgctgcctg tgacccgctc gtcgtacacc tcgtgcgcca
      421 cgagctcgca ccccctcttt cccaagaccg atgacatgag gaccaaggcc ggcagaccgc
      481 acacgttgta ggcgtcgcgg caggatgcgg cgcggccgaa gaggccggac gcatccccct
      541 ccatgagaca ccccaagagc gcacggtcgt gctccatggc cttatccagg agacgatccg
      601 ccctctggtc gtggccgaac ttgagcccca cgtgcgagaa gtccaccccg gcaacgacga
      661 gcaccctccc ggggtccgcc gagacgacct cgcagagggc gtccctgaac gcgatgaagc
      721 gcgggtcctc ggcggggtcg cggcccgatc tgacgaactc gtggaaagat ccgcacagaa
      781 cgggcacgat cctcgttcgc gacaccccca ggtagtaggc gatgaagagg gcctggaact
      841 cgatggagtg ctctgacttg tgatcgaggt cggtgggcgc gacgccgccc ggcggcatgc
      901 ccctggaaag cctcctcacg atctccctgt gggtcttgag gacgccgaag ggggtctcga
      961 agtccttttc cgtgaggcag tagaggccgt ccgaaccgta gtggttcacc ccgaggacga
     1021 ccaccgtgtc gaacgaggcg tccctcagcc tgcgatacac gtccacgtac gtcctgaagg
     1081 cgaccttgag gtcgatgtgc ggcgccacga cgcccgatat cccgtccttc gtgcccgccg
     1141 gcagcggggg gaggctcctc tcgagctcct ctatcgacgc tgcgagcgcc tcagggtccg
     1201 cctcgtaaga cctgccgtcg agtatcgtac ggcggatgcc cgcgcgcagg aactcctcct
     1261 cgacggccct cctcctgtcc ctgaagcgcc ggctgtccag cagcaaagcc tcatcaagcc
     1321 tctccaggaa agcctcgatg tgttccatgg gcacgatgga ccctccagag acgcgcatga
     1381 gctccgcctg gatgtccagg agatccctcg tgccgtccat catggccaac acgctcagcg
     1441 cacgcctgtc cacggcgaac ccctcgtcgg ccatcctcaa gggatccatg aatacgacca
     1501 tctcgcgccc ttctaccgtg acgaatacgg gctggatgtc gctccgcatg cgggggtgcg
     1561 tcgatctttc catgtcggga gagcctgcct tgcctcgccc ctcctcaggg gtggtgaccc
     1621 ggcgcaggtc cacaggcagc cgtgtcgaaa cgaaaaaacg gctgcgtccg ggggaccgcg
     1681 cctactttgc ggccttcctg gccgtcttgg cgccccgcgc cgcaggcgct tccggcgcct
     1741 tcttcctctt cggccgggtc ttcccgaagg tgcccctgaa gatctttccc ctcttgctcc
     1801 gctggtcacc cttgcccatg gatcggtcct ccttcctggc gtgctcgggc cttcgtctct
     1861 tgaagagccc tcgccgggcc tttcaaggct ctctctcgac gcccggcgct cgaacgcata
     1921 actattcaat tttgttcgca cgcaacacca atgtcaaggg ggaacaggtg cgggcgcaga
     1981 cgcaagaagg ctggagccac gacgacgccc tcggcgtcac gggcgctaaa gggtgagggc
     2041 tccaggcagc cttcaaggaa ggcgttcgtg cctgggggtg tgatacgccg tgagtgcacg
     2101 aaggtcaccg gcaagggttt ggtggggcag gtgtccctgc tggtttcggg gccgtcgtga
     2161 catcagtgca tctcccgtgc gaggcgcagg aggtgctccc gggtgaacac gtaggtcttg
     2221 ttgcagaagt ggcagaagat gtcgaacatc tcctcggtgg cagctatttc ctcgatctgt
     2281 ctcttcccca gggtgacgag cgcctgttcg atgcgaccca ccgagcatga acaggcgaaa
     2341 gacacccgcc tcgtctcgat gatcctgaag ggggtctggg tgaagatcat gcccaggatg
     2401 tcctccggcg ttttcccctg catgaggagc gtggtcaccc ccggcaggga ctggatggtg
     2461 cgctcgatgg aggagatcag ggcgtcgtcc ctgggcggca gggactgcac gaggaacccg
     2521 ccggaagcgc ccacgcgctc gtcggcctcc acgtacacgc cgagccccac cgcggacggc
     2581 acctgctcgg actcgctgag gtagtaggct atgtcggacg cgatctcccc ggtgtggagt
     2641 tccacgatgc cgctgtaggg ggccctgatc ctcaagtccc ttgtcacggt tagaaaaccg
     2701 cgcctgccga tggcgcctcc cacgtcgagc ttgccgtcca cgggagggag atcgacgcga
     2761 ggcacctgga catagcctcg caccgtcccg tccccctcgg cctccaccac gatcttcttg
     2821 agcggtccgt cgcccgtgaa cttgatggcc acgcgctgcc cctcgtccag gagagcgccc
     2881 agcaacccac ccccggtcag cgccctgccc agggccgccg tcgccgtggg gcacgtcttg
     2941 tgccttctcc tggcatcctc tacgaggttc gtggtgacgc aggcaaggcc gatcacgttc
     3001 ccgccttcgg tcatgcccct cacgaggtaa tcttccatct gtccgtgtcc cccgaggcca
     3061 atcctgtatc aggaccggcc atccaggtca atccgatccg gaagagacaa ggaacggcca
     3121 caaggatgcc ttccggcgat gcggacacgg gggcttgccc tgcctccccc gaaaagac
//
LOCUS       DAOSCR010000311         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19589_length_3173_cov_3.747274, whole genome shotgun sequence.
ACCESSION   DAOSCR010000311 DAOSCR010000000
VERSION     DAOSCR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19589_length_3173_cov_3.747274"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="trmFO"
                     /locus_tag="PLT69_08100"
     CDS             <1..837
                     /gene="trmFO"
                     /locus_tag="PLT69_08100"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOJ14436.1"
                     /translation="SPVVDAQSIDMEHAFFASRWDAPSMDYLNCPLDETTYRAFVSEL
                     LKADRAKAHLFEDARYFEACLPVEVLAGRGADALRFGPMRPVGLLDPRTGRRPFAVVQ
                     LRRENLAGDAYTMVGFQTRLTYPEQKRVISLIPALRHAEFFRFGSIHRNTYVDSPQVL
                     NRDLSLKAAGNVYLAGQITGVEGYVESSATGIVAGLSVLARIKDAAFSPPGPETAIGA
                     LIAYITSPTQAPFQPSNINFGIMAALDAPRKRRAELLSSRALSSFERWMENTMRALGE
                     RA"
     gene            complement(1216..1416)
                     /locus_tag="PLT69_08105"
     CDS             complement(1216..1416)
                     /locus_tag="PLT69_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOJ14437.1"
                     /translation="MATGTVKWFNEKKGYGFITQDGGNDVFVHYSAIKMDGFKTLAEG
                     DKVNFEVTNGPKGPQASNVTKV"
     gene            1778..>3173
                     /gene="pepD"
                     /locus_tag="PLT69_08110"
     CDS             1778..>3173
                     /gene="pepD"
                     /locus_tag="PLT69_08110"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOJ14438.1"
                     /translation="MNALAGLSPEALWEHFLAVSRIPRGSGNEGAVVTYVATVASTAG
                     LPFRIDRAGNIIVKKRGARGPAGVALQSHLDMVCEKREGSPHDFEKDPLVLVRDGPWI
                     KARDTTLGADNGIGVAAMLAIMTSNDIVHPDLELVFTVDEEAGLVGAQSLERQDITSR
                     TLINLDSEEEGTFCIGCAGGIHTELRLEPDFMSVPEDTSAVLVSVSGLTGGHSGVDIH
                     LGRPNAIKLIGRFLHAVDSAYSIHLADLSGGTRHNVIPRDARALIHASPADIGALHNE
                     AAHWNGILKTEYEHADHRVALVLEESSEQAARVLTPESTKTVIRLVQALPHGPLRFNP
                     EWEGVVDTSTNLAVCRFDGDEFLVVTSQRSTTPSSLHDAASQVASIGELAGCTVSHLG
                     AYPAWRPDRNSAILSTCRDVYREITGREPNVKVIHAGLECAVIGEKFPSMDTISFGPT
                     IRNAHSPDECVSIPS"
BASE COUNT          570 a         1049 c         1019 g          535 t
ORIGIN      
        1 tctcccgtcg tggacgcgca gagcatagac atggagcacg ccttcttcgc ttccaggtgg
       61 gacgccccgt ccatggacta cctcaactgc cccctcgacg agaccaccta cagggccttc
      121 gtgagcgagc tcttgaaggc cgacagggca aaggcccacc tcttcgagga cgcccgctac
      181 ttcgaggcct gcctccccgt ggaggtgctc gcaggcaggg gggcggacgc cctcaggttc
      241 ggccccatga gacccgtcgg ccttttggac cccaggaccg ggcggagacc gttcgccgtc
      301 gtgcagctga ggagggaaaa cctcgccggc gacgcgtaca ccatggtggg gttccagacc
      361 aggctcacct accccgagca gaagcgggtc atctccctga tcccggcgct caggcacgcc
      421 gagttcttcc gcttcgggag catccacagg aacacctacg tggactcgcc ccaggtcctg
      481 aacagggact tgagcctcaa ggccgcaggc aacgtgtacc tggcggggca gatcacgggg
      541 gtcgaggggt acgtggagtc gtccgccacg gggatcgtcg ccgggctgtc ggtccttgcg
      601 cgtatcaagg atgccgcatt ctcgcctccc gggccggaga ccgccatagg cgccctgatc
      661 gcctacatca cgagccccac ccaggcacct ttccagccat cgaacatcaa cttcggcatc
      721 atggcggccc tggacgcacc caggaaacgc cgggcagagc tcttgagctc gagggcgctc
      781 tcctcgttcg agaggtggat ggagaacacg atgagggcac tcggggaacg ggcctgatgg
      841 cccgtgtcca ggccgcatct tggagtgcac gtcctcaccc ctggcctgtg acctcacgca
      901 ccggtcctca cgcgcggtca cgggcccgga gggacccctc gagcgctccg gggatgtctc
      961 gcgacggggc agagcccgcc accttgacgc ctgtggacgg acgctcctcc acccggtgca
     1021 tgggacggca catgggcgac gctggcgaag gggcgggact cgtgtcgata cggcgcaggc
     1081 gcaaagaggg cgctcgaaga caggggggac tcgaagaacg caggccttga gaccgtctcc
     1141 agcctcgcgg tcgctgcggg tgtgttcagg aaccggggcg ccgccggtgg caggccgcgc
     1201 cccggctggc ggcagttaca ccttggttac gttcgatgcc tggggacctt tcggcccgtt
     1261 ggtcacctcg aagttgacct tgtctccctc ggcaagggtc ttgaaaccgt ccatcttgat
     1321 ggcggagtag tgcacgaaga catcgttgcc gccatcctga gttatgaaac cgtacccctt
     1381 cttctcgttg aaccacttga ctgttcctgt agccattcat acctccgttt tttcaggaca
     1441 cgacccttgg cgagagggcg gaccaggtgt tgcagtctgg aacgtgggta ttaccagaaa
     1501 ccggggaaaa cttaggaagg gttttaccgc aggcttgcat gataatgtcc atatccaggc
     1561 tatacgtgtc tatatcgtca aatccggctt tctgtcaagc ctcatcgagc gtccatgatc
     1621 gtgttcccat gccgcggcgt cctcagggaa aggccacgct cgccgagcga cgcgccagtc
     1681 ggagccccgt cctcccgaag gcacttcgtg cttgcccggg gaccgtcgag ctggtatccc
     1741 ctaggtggat gccgtgttct gagggaggtg aatcgctgtg aacgctctcg ctggattgtc
     1801 tccagaggcc ctctgggaac atttcctcgc cgtctcgagg atacccaggg gctccgggaa
     1861 cgaaggggcg gtggtcacct acgtcgcgac ggtcgcttcc accgcgggcc tgcccttcag
     1921 gatagacagg gcgggaaaca tcatcgtgaa gaagcgcggc gcgaggggcc ccgcgggggt
     1981 ggccctgcag tctcacctcg acatggtgtg cgaaaagagg gagggatctc cccacgactt
     2041 cgagaaagac cctctggtct tggtgaggga tggaccctgg atcaaggccc gtgacaccac
     2101 cctgggcgcg gacaacggga taggcgtcgc tgccatgctg gcgatcatga cgtcgaacga
     2161 catcgtccac cccgacctcg agctcgtctt caccgtcgac gaggaggcgg ggcttgtggg
     2221 cgcacagtct ctcgagaggc aggacataac gtcgcgcacc ctcatcaacc tcgattcgga
     2281 ggaagaaggg acgttctgca taggctgcgc gggcggcatc cacaccgagc tgaggctcga
     2341 gcccgacttc atgtcggtcc cggaggacac ctccgcggtg cttgtgagcg tgtcggggct
     2401 cacggggggt cactcgggcg tggacatcca cctcggcagg ccgaacgcga taaagctcat
     2461 cggccggttc ctccacgccg tcgattccgc atacagcatc cacctggccg acctctccgg
     2521 cgggacgagg cacaacgtca tccccaggga cgcccgtgcg ctcatccacg catccccggc
     2581 cgacataggc gcgctgcaca acgaggcggc ccactggaac gggatcctca agaccgagta
     2641 cgaacacgcg gaccaccgcg tggccctggt cctcgaggaa tcgtccgagc aggcggcacg
     2701 cgtcctcacg ccggagtcca ccaagacggt gatcaggctc gtccaggcgc tccctcacgg
     2761 ccccctcagg ttcaacccgg aatgggaagg cgtggtggac acgtccacga acctcgcggt
     2821 gtgccgcttc gatggagacg agttcctcgt cgtcacgagc cagaggagca cgacaccgag
     2881 ttccctgcac gacgcggcgt cgcaggtggc gtccatcggg gagctcgccg ggtgcacggt
     2941 ctcccacctg ggcgcctacc cggcctggcg gccggacagg aactcggcca tcctgtccac
     3001 ctgccgcgat gtctaccggg agatcaccgg cagggaaccg aacgtgaagg tgatccacgc
     3061 cggcctcgag tgcgccgtca tcggcgagaa gtttccgagc atggacacca tctcgttcgg
     3121 cccgacgatc cggaacgccc actcgcccga cgagtgcgtg agcatcccgt cgg
//
LOCUS       DAOSCR010000312         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19620_length_3170_cov_3.490209, whole genome shotgun sequence.
ACCESSION   DAOSCR010000312 DAOSCR010000000
VERSION     DAOSCR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19620_length_3170_cov_3.490209"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..716
                     /locus_tag="PLT69_08115"
     CDS             261..716
                     /locus_tag="PLT69_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ14439.1"
                     /translation="MITITRPSWTRDIWGWDPWRELDRLHREVSGIFGLPRLRRLAAY
                     PPMNVHTADDDVVVTSELPGVDPSEVEITVSGDTLSIKGSRKPRELKEGEQWHRRERG
                     FGEFFRTVQLPFTVDASKVSAEYTNGVLKIVLPRSEADKPRKIAVKSTN"
     gene            740..1144
                     /locus_tag="PLT69_08120"
     CDS             740..1144
                     /locus_tag="PLT69_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ14440.1"
                     /translation="MEDAKKNIEKAQAKDASLAERTKDRVIFTPAVDITEDSNNLYIY
                     ADVPGATEKGVNITLENDVLTIEAQVEEAPMEGYTRTYSEYATGDYYRAFTLNEAIDR
                     DKIEATLKDGVLGIVLPKAEAVKSKKIPIKAG"
     gene            1172..1675
                     /locus_tag="PLT69_08125"
     CDS             1172..1675
                     /locus_tag="PLT69_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ14441.1"
                     /translation="MRYLTPFRFGRRNVPEAREFEAHPLDLFQREMNRLFDEFFKGTG
                     LRRWAEDFESFGSFTPQVNMTEDEKSIVVSAELPGMDEKDIEISLSKDSLTIKGEKKE
                     EAEHKGKEAYCIERSYGSFTRVLPLPKEVNPDKAEATFKKGVLTVTLPKVEKEKGSQK
                     KIKIKAD"
     gene            2454..>3170
                     /locus_tag="PLT69_08130"
     CDS             2454..>3170
                     /locus_tag="PLT69_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOJ14442.1"
                     /translation="MDVNRFTQKSQEAIANAQNTAVRFSHPEIDVEHLLLALVEQEDG
                     LVPRLFAKMDVPMASFSAELVKELERRPKVSGPGVEPGKALITQRLSRVLVAAKEEAA
                     RLKDEYVSVEHLLFAMIDEGRSSAAARILARFNITRERILQALTSIRGNQRVTSDNPE
                     AAYEALQKYGIDLVSQAQLGKLDPVIGRDDEIRRVIRILSRKTKNNPVLIGEPGVGKT
                     AIVEGLAQRIVRGDVPEGLKD"
BASE COUNT          674 a         1021 c         1018 g          457 t
ORIGIN      
        1 tctcgtcctc ggcggtgtca ccgggcatgc cgttcgccga ccccccttca ccagggtcac
       61 gaggcggacc gctgcccgcc cgggacacgt tgcccccgga tcccctcggc agccgacgac
      121 cccccgttcc cacgatggcg ccagggggcg gcaggcatct ttcaggcccg ctcgccggta
      181 cagccacccc ttgaaatccc gggaagagac cctactgtac cattggaaat cgagaaacac
      241 cccacaggag gtgaatgcac atgataacca tcacccgacc gagttggacg agggatatct
      301 ggggctggga cccctggcgg gagctggacc gcctgcacag ggaagtcagc ggcatcttcg
      361 gcctccccag gctcaggagg cttgccgcct acccaccgat gaacgtccac accgcggacg
      421 acgacgtcgt cgtgacgtcc gagctgcccg gcgttgatcc gtcggaggtg gagatcacgg
      481 tgagcggcga cacgctctcc ataaagggtt cgcgcaaacc gagggagctc aaggagggag
      541 agcagtggca ccgcagggaa cgtgggttcg gcgagttctt caggaccgtg cagctgcctt
      601 tcaccgtcga cgcgtccaag gtgagcgccg agtacacaaa cggcgtcctg aagatcgtcc
      661 tgccgaggtc agaggccgac aagccgagga agatagcggt caagtcgacg aactgagaag
      721 gcagaaggag gtgacgacca tggaagacgc caagaagaat atcgagaagg cccaggccaa
      781 ggatgcatcc ctggccgaga ggaccaagga cagggtgatc ttcacgccgg ccgtcgacat
      841 caccgaggac tcgaacaacc tctacatcta cgcggacgtg cccggtgcga cggaaaaggg
      901 cgtgaacatc accctcgaga acgacgtctt gaccatcgag gcccaggtgg aggaggcgcc
      961 gatggaaggg tacaccagga cctactctga gtacgcgacc ggcgactatt accgggcatt
     1021 caccctgaac gaggccatag accgcgacaa gatcgaggcc acgctcaagg acggggtgct
     1081 cggcatcgtg ctccccaagg ccgaggcggt caagtccaag aagatcccga tcaaggcggg
     1141 atgaccaaac cacgaaaagg agggacagac catgagatac ctgactccgt tccgtttcgg
     1201 ccgcagaaac gtcccggagg cccgcgagtt cgaggctcat cccctggatc tgttccagag
     1261 ggagatgaac aggctgttcg acgagttctt caagggaacg ggcctgaggc ggtgggccga
     1321 ggatttcgaa tcgttcgggt cgttcacccc ccaggtgaac atgaccgagg acgagaagtc
     1381 catcgtcgtg agcgcggagc tgccgggcat ggacgagaag gacatcgaga tcagcctcag
     1441 caaggactcc ctgaccatca agggcgaaaa gaaggaggag gccgagcaca agggcaagga
     1501 ggcctactgc atcgaacggt cgtacggctc cttcacgagg gtcctgcccc tgcccaagga
     1561 ggtgaacccc gacaaggccg aggccacctt caagaagggg gtgctcaccg tgaccctgcc
     1621 caaggtagag aaagaaaagg gatcccagaa gaagatcaag atcaaggcgg actgagaacc
     1681 ggcgccccgg cgaggcgtgt caccgccttc ggcccgcacg cgacgcccgg accacgagga
     1741 acccgggcgg ctcttgggcg cccgatccga ggggggcctt ccccggtccc cctcgtgctt
     1801 ttcgctcgtc attgcgcaac cggtctcaat tcccctctcg atagtggcct tctcctatac
     1861 ccctcgtgtt tctcgttcgc catgcccggt tacgggcgtg cattcctgca gggggatcga
     1921 tgggtctgcc tgagggagga aaacgcctgg tgaagcctgg aacaacgttg cccgcgcgag
     1981 gtcgacccat acgccgagat cgacgatcag cgccatggac caccctccat gcgccagggg
     2041 cgtgacatgc cggcaggccg ggtatttcag gctcggcctg acccaccttc catgcgcccg
     2101 gggggtgaca acgcatctcg gccttcgccc tccccgccac gccgctctca ccccgagggg
     2161 tcgcttcgcg ccgggggcct tcgggcccga gccgcgttca cctcgccgag agggtgctgc
     2221 aggtgcggcg ccaagcctgc ccggcacccc gaccggtgca tgtcttctcg gccggcgcat
     2281 cgtccagagc ggaacgaccc gccctcctgt gcaccctgcg aggtacaggc ggccatccgg
     2341 cttgaaaccc caagcacgct cgcctacata agaagtgagt cgagggcttg caggcatgcc
     2401 ctcgacaaag agacgcagtc cggcaaagga cactacccag gagagtgaaa gagatggatg
     2461 tgaacaggtt cacgcagaaa tcacaagagg cgatagcgaa cgcccagaac acggctgtcc
     2521 gcttctccca cccggagatc gacgtggagc acctgctgct cgcgctcgtc gagcaggaag
     2581 acggcctcgt gccgcggctc ttcgccaaga tggacgtgcc gatggcgagc ttttcggccg
     2641 agctcgtcaa ggaactcgaa cgcaggccga aggtgtcggg ccccggcgtc gagccgggca
     2701 aggctctcat cacccagagg ctgagcaggg tgctggtcgc cgcaaaggag gaagcggcgc
     2761 gcctgaagga cgagtacgtc tcggtcgagc acctcctctt cgccatgatc gacgagggca
     2821 ggtcgtctgc tgcggcccgc atcctcgccc ggttcaacat caccagggaa cggatcctcc
     2881 aggcgctgac ctcgatcagg ggcaaccagc gcgtgacgag cgacaacccg gaggccgcct
     2941 acgaggccct ccagaaatac ggcatagacc tggtctccca ggcccagctc ggcaagctcg
     3001 accccgtcat cgggagggac gacgagatca ggcgggtgat ccgcatcctc tcccggaaga
     3061 ccaagaacaa ccccgtgctc ataggagagc cgggcgtcgg gaagacggcg atagtggagg
     3121 gcctcgccca gcgtatcgtc aggggggacg tgcccgaggg cctcaaggac
//
LOCUS       DAOSCR010000313         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19643_length_3167_cov_3.431234, whole genome shotgun sequence.
ACCESSION   DAOSCR010000313 DAOSCR010000000
VERSION     DAOSCR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19643_length_3167_cov_3.431234"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLT69_08135"
     CDS             complement(<1..1038)
                     /locus_tag="PLT69_08135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ14443.1"
                     /translation="MARILVTNFHKGPGTGHTTYIRTLTRLSGESEHVVGVAVAERSR
                     LYRLLKEHGHPHLFACEFPNRVLKRKKDFLSSIRRFRDIVREFRPDIVHTNGGADLFV
                     SLWSHPVARPYRVVRTHHANRKIRSDPYHRLVYGRLTSANIFVSDSAYEKAVSRGVVP
                     HNALVIKNGVDVDRFRPMAKDDGLARRFGIDEGTFCFGTSGGTKFYKRVDTIIKAASL
                     VKTQRRFAILVLGGPNPELEEMARALIPGKFVHLGFQEDVVPYISLFDVGFILSDAIE
                     TISFAAREMMSMGKPLISSTYSGLKENVIHGTNGLLVEPGDVRGIAEAMGAFLAMDVE
                     TLGRFSENARLH"
     gene            1207..1911
                     /locus_tag="PLT69_08140"
     CDS             1207..1911
                     /locus_tag="PLT69_08140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ14444.1"
                     /translation="MQIIDFLRIIPALITVPMRKLSSFAIPLALGRFKGSRAYWVRRY
                     RKGGGSGVGSYNELAEFKAEVLNGFVKEHNIETVIEFGCGDGNQLRYACYPSYLGFDI
                     SPEAVSICRRIFQQDENKRFFLLEEYSGQKADLTLSLDVIYHLTEDDVFHDHMDMLFN
                     ASKRFVIIYSSNTDDNSANHSRHVKHRKFTSWVEKRKPEWRLTRHIKNRYPYDANNRK
                     SSWSDFFIYEKARDLD"
     gene            2050..3024
                     /locus_tag="PLT69_08145"
     CDS             2050..3024
                     /locus_tag="PLT69_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOJ14445.1"
                     /translation="MERHPMNILFVKLGALGDVINTLPLAITLKERMGARIHWLVEPL
                     SLAVVSGHPAVDDVVLFDKNRWPRALPGVLSRIRQRRYDIALDLQRTLKSGLFCTASS
                     STRRIGFDRARCKELTWMLPFERIRAGDPAKHMVYQYLEFATHLGVHEHEVRWDIPVA
                     GRVPFGITGDYIVLNIGATKPANRWTPEGFARLAVLIGRRLGMTPVLTGGREDVPMAG
                     GIASLAGGYVVDLAGKTSIPELKEVLAGARAVVSCDTGPMHLAVALGKKVVVLVGPSN
                     PQRTGPLRGSVVRLDLPCMPCNRRKCATPLCMLGISPELVMKHLEEII"
BASE COUNT          639 a          963 c          947 g          618 t
ORIGIN      
        1 gtgcagcctt gcgttctccg agaacctccc caacgtctcc acgtccattg caagaaaggc
       61 gcccatggcc tcggctatcc cccggacgtc cccgggctcg acgagaaggc catttgtgcc
      121 gtggatcacg ttctccttca ggcccgagta ggtgctcgat atgagcggct tgcccatgga
      181 catcatctcc ctggcagcaa aggagatcgt ctcgatcgca tcgctcagga tgaacccgac
      241 gtcgaagagc gagatgtacg ggacgacatc ctcctggaag cccaggtgga cgaacttgcc
      301 cgggatgagg gcgcgtgcca tctcttcgag ctcggggttc ggaccgccga gcaccaggat
      361 cgcaaatctc ctctgggtct tcacgaggga ggcggccttg atgatggtgt ccaccctctt
      421 gtagaacttc gtgccgccgc tggtgccgaa acagaaggtt ccctcgtcga tcccgaacct
      481 gcgggcgagc ccgtcgtcct tggccatggg cctgaacctg tccacatcca caccgttctt
      541 tatgacgagt gcgttgtggg ggaccacccc cctcgagacg gccttttcgt acgcggagtc
      601 cgaaacgaag atgttcgcgc ttgtcaggcg gccgtagacg agccgatggt acgggtcgct
      661 ccgtatcttc cggttggcgt ggtgtgtcct tacgacccgg tacggccttg cgacggggtg
      721 gctccagagg ctgacgaaga ggtcggcgcc tccgttggtg tggacgatgt ccggcctgaa
      781 ttccctcacg atgtccctga acctcctgat gctggatagg aagtccttct tgcgcttcaa
      841 caccctgttg ggaaattcac aggcaaagag gtgggggtga ccgtgctcct tcagcagcct
      901 gtagagtctc gacctctccg cgacggcgac acccacgacg tgttcggatt ccccggacag
      961 cctggtgagc gtcctgatgt acgtcgtgtg ccctgtgcct gggcccttgt gaaagtttgt
     1021 cacgaggata cgcgccatgg gacccgctcc ctccttatcg tccttcccgg cccttcctgg
     1081 caccggcctt ccatgtacca agtcggcgtg gtatgttcaa caagaaagcc catgcaagca
     1141 tgcgagtggc tggacacccc tcaagaaagc ggggcccacg aaaaacgggt gcggatggag
     1201 atcggcatgc agattataga ctttctcagg atcatacctg ccttgatcac ggtaccgatg
     1261 aggaaactct cctcttttgc gataccattg gccctcggcc gtttcaaggg ttcgagggcg
     1321 tactgggtga ggagatacag aaagggggga ggatcggggg tcggttcata caacgagctg
     1381 gccgagttca aggcagaggt tctcaatggg ttcgtgaaag aacacaacat agagaccgtc
     1441 atcgagttcg ggtgtggtga cggcaatcag ctgagatacg cttgctaccc ttcgtacctg
     1501 ggtttcgaca taagcccgga agcggtctcg atatgcagga ggatcttcca gcaagatgaa
     1561 aacaagcgct tcttcctcct cgaggagtat tccggtcaaa aggctgatct gaccctgtct
     1621 ttggatgtta tctaccacct cacggaagac gatgtcttcc atgaccacat ggacatgttg
     1681 tttaacgcct cgaagcgctt cgtgatcatc tattcatcca acacggacga caacagcgcg
     1741 aatcattctc gacatgtaaa acacaggaag ttcacttcat gggtcgagaa aaggaaaccg
     1801 gagtggagat tgacgaggca tataaaaaac aggtacccgt acgatgcgaa caacaggaag
     1861 agttcctggt cagatttctt catctatgaa aaggcccgag atctcgattg aaagccacgc
     1921 ctcgtaccag gagaccgacc ctgttctcca tggtatcgtc cggatagtct ccgcttccag
     1981 aacgcgaggc cggtgcacat cccgctcccg ttcgaggggg tcacgttggg gcggacagga
     2041 gaacgagaca tggaaaggca ccccatgaat atactcttcg tcaagctggg ggccctggga
     2101 gacgtgatca acacgctccc cctggcaatc acgctcaagg agcgcatggg cgcccgcatc
     2161 cactggctcg tggaaccgct gagcctcgcc gtcgtgtcag gacaccccgc ggtcgacgac
     2221 gtcgtcctct tcgacaagaa ccgctggccc agggcccttc ccggcgtcct gagccgcatc
     2281 agacagcggc gctacgacat cgcgctcgat ctccagcgca ccctcaagtc aggtttgttc
     2341 tgcacggcat cgtcgagcac cagacgcatc ggcttcgaca gggcccgctg caaggaactc
     2401 acgtggatgc tgcccttcga gcgcatccgt gcgggcgatc ccgccaagca catggtctac
     2461 cagtacctcg agttcgcgac ccacctgggt gttcacgaac acgaggtccg ctgggacatc
     2521 cccgtggcag gcagggtgcc cttcgggatc accggcgact acatcgtgct caacataggc
     2581 gccacaaagc cggccaacag gtggacgccc gaggggttcg cgcgcctcgc ggtcctgatc
     2641 gggcggagac tgggcatgac ccccgtcctc accgggggtc gcgaagacgt ccccatggcg
     2701 ggcgggatcg catccctggc aggcggatac gtggtcgacc ttgcgggcaa aacgagcatc
     2761 cccgagctca aggaggtcct cgcgggggca cgggcggtag tcagctgtga caccgggccc
     2821 atgcacctgg ccgtggccct cgggaaaaag gtcgtcgtgc ttgtcggacc ttccaatccc
     2881 caaaggacgg gccctctcag ggggagcgtg gtgaggctgg acctcccctg catgccatgc
     2941 aacaggagga agtgcgcgac cccgctctgc atgctgggga tatcccccga gctcgtgatg
     3001 aaacacctgg aggagatcat ctgagggaaa gcgcaccccg gccgatgagg tcatggctga
     3061 ccgccggcct cggtctggcc ttctcccacg acggtcaccc gccaccgggt gaaatccctc
     3121 cgcgccccgg tcgaggggtg gtgtcaagaa tgtttcgcac ttttgat
//
LOCUS       DAOSCR010000314         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19747_length_3154_cov_3.044530, whole genome shotgun sequence.
ACCESSION   DAOSCR010000314 DAOSCR010000000
VERSION     DAOSCR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19747_length_3154_cov_3.044530"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLT69_08150"
     CDS             <1..349
                     /locus_tag="PLT69_08150"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOJ14446.1"
                     /translation="KAPRIFYVNWFRKDDAGRFMWPGFGENSRVLKWMCERVDGAVGA
                     QETTIGLMPLEKDLDLQGLALAPETLRELLRVDDDAYRAEASSIEKHLEQFGSRLPER
                     LRAQLLALRERLG"
     gene            979..2067
                     /locus_tag="PLT69_08155"
     CDS             979..2067
                     /locus_tag="PLT69_08155"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ14447.1"
                     /translation="MEAVDNREERLREWASLGARPMGYLCTYTPVELIHACGFLPVRL
                     WGGAGQVRKAYSHVPPFVCPYLRLVVERAACSTFDFLVGIVQGYTCDAACGVVNVWKN
                     TFGGGICMNLPLPYNESASARTFFASCAHDLASRLEALGGCFSEERLGRSVGLYERIR
                     ESLARVGEMCSGESACLSAMDISVVVQAFFSTPPETYLAMLQELEALLQGAPRVRREG
                     VPVLVSGSVLEDAGVIGLIEETGFRVVACDLCTGIRAYTPHAGTGSTPMERLASRHAA
                     RFPCPSRSRPQDRVPLLAGLARDSGAAGVIFLIQKFCTPHLADHPLVERGLRDAGIPS
                     ILIELDVEDTIDARTANRLETFAALVGG"
     gene            2069..>3154
                     /locus_tag="PLT69_08160"
     CDS             2069..>3154
                     /locus_tag="PLT69_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ14448.1"
                     /translation="MAKTGKSVKRLASARELATLVAGYYEECHRAKAEGVPVGWMPPM
                     NGGIEIFYAMGLQPVFPENFSPVCAAFGAALSHFEAAEAMGYSSDLCGYLRNIVGYVH
                     SDMHGPGVPLQGLPEPDMIMSFGGGCVPAMKIFHILEHRFQGAAVFRADMPQVAVEEI
                     REHHVEYATSEMHRFIDFLERTTGRKLDMERLREVVALSDEACRLWDEIMSYRRFSPA
                     PITASEIGIMFVMVTRQGTKTAVEFLERVRDEVAQRAHKGMGAVEDERVRLFWDNIPL
                     WHNMGIFNYFESSGGVFVAETYSAAWSLRLDPDDPVRSLALKSLMSYPLVSCVSIDRR
                     KEMVLKACRDYAIDGAVFHRNRSCVPIS"
BASE COUNT          514 a          982 c         1099 g          559 t
ORIGIN      
        1 caaggccccc cgcatcttct acgtgaactg gttcaggaag gacgacgcag gaaggttcat
       61 gtggcccggc ttcggcgaga acagcagggt cctcaagtgg atgtgcgagc gtgtcgacgg
      121 ggccgtgggc gctcaggaga ccaccatcgg cctcatgccc ctggagaagg atctcgacct
      181 gcagggcctc gccctggcgc ccgagaccct gagggaactc ctcagggtgg atgacgacgc
      241 ctaccgggcg gaggcatcga gcatcgagaa gcacctcgag cagttcgggt caaggcttcc
      301 cgagcgcctg cgcgcgcagc tcctcgccct gagggaaagg ctcggctgac cattctcctg
      361 caaggggaac agggcgccgc ctgtcggtgc ccgttgcagg aaggatgccg cctgggcgtt
      421 gcggtatgcg ggcatgtgtg ttgcccctgt tgccactggg tgttcttcgg gacgggaagg
      481 tctcggccgt tgggcttacc cgaaccgtgg gggccgggcg ggaggccgtc accaccgtgt
      541 cgcgcgcagg agtgtcggca tacggcgcgg cgcactcttt cagagtgacc atggattcag
      601 ggggattcga gtgtcggcgc gatgacgccg cccccctgca agcaggggac ggggccctac
      661 atctggaagg agggcgtggc gtttgacgca tcccccatgc aaccagcggg cggggtcgga
      721 ttttccctcc cgggccgcgc ctgtaccgtg gctgaagatg cgcccggctg gggtgccacc
      781 ggcccgagcg gccgcctcga gaccgtggcg gccgtgcctg cctcgacggt ggggcaccct
      841 cgtccctcgc ctgttcatac cctgtcgtcc atcgcgccac cgcaagggct cgagcgttga
      901 cgcacccggc tttatgttgt aatacaatca tacatgatga acaggcatga tggatgctcg
      961 ccgttggtgg cgcctttcgt ggaggcagtg gacaacaggg aggaaagact ccgggaatgg
     1021 gcgtctctgg gcgcgcgccc gatgggctac ctgtgcacgt acacgcccgt ggagctcatc
     1081 catgcgtgcg gtttcctccc ggtaaggctg tggggtggag cggggcaggt gagaaaggcc
     1141 tattcccacg tgccgccgtt tgtctgcccg tacctgaggc tcgtggtcga gcgtgccgca
     1201 tgctccacgt tcgacttcct cgtgggcatc gtccagggct acacctgcga cgcggcctgc
     1261 ggggtcgtga acgtctggaa gaacaccttc ggaggcggta tctgcatgaa cctgccgctg
     1321 ccctacaacg agtccgcctc tgccaggacc ttcttcgcct cgtgcgcaca cgacctcgcc
     1381 tccaggctcg aggccctggg agggtgcttt tccgaggaga ggctggggcg ttcggtgggc
     1441 ctctacgaga ggatcaggga gtctctggcc agggtgggcg agatgtgcag cggcgagagc
     1501 gcctgcctga gcgcgatgga cataagcgtc gtggtgcagg cgttcttctc caccccgccc
     1561 gagacctacc ttgccatgct gcaggagctt gaagcccttc tccagggcgc gccgcgcgtg
     1621 aggcgggaag gggtcccggt tctcgtctcg ggcagcgtcc tggaggatgc aggcgtgatc
     1681 gggctcatcg aggaaacggg gttcagggtg gtggcctgcg acctgtgcac cgggatacgc
     1741 gcctacacgc cccatgccgg gacagggtcg acgcccatgg aaaggctcgc ttccaggcac
     1801 gcggcgcgct ttccctgccc ttccaggtcg aggccccagg atcgcgtccc gctgctggcc
     1861 gggctcgcga gggactcggg cgcagccggg gtgatcttcc tgatccagaa gttctgtacg
     1921 ccgcacctgg cagaccaccc cctggtggag cgcggcctca gggatgccgg catcccgtcc
     1981 atcctcatcg agctggacgt ggaggatacg atcgacgccc gaacggccaa ccggctcgag
     2041 acgttcgcgg cgctcgtggg gggctgacat ggcgaagacg ggcaagtcgg tcaagaggct
     2101 ggccagcgca agggaactgg cgacgctcgt cgccgggtac tacgaggaat gtcaccgggc
     2161 gaaggccgag ggggtgcccg tggggtggat gccccccatg aacggcggga tagagatctt
     2221 ctacgccatg ggcctgcagc cggtgttccc cgagaacttc tctcccgtgt gcgcggcctt
     2281 cggcgcagcc ttgtcgcatt tcgaggcggc tgaggccatg gggtactcga gcgacctgtg
     2341 cgggtacctc cgcaatatcg tggggtacgt tcattcggac atgcacgggc cgggtgtccc
     2401 cctgcagggg ctccccgagc ccgacatgat catgtccttc ggcggcgggt gcgtcccggc
     2461 gatgaagatc ttccacatcc tggaacacag gttccaaggc gccgccgtgt tcagggccga
     2521 catgccgcag gtggcggtgg aagagatccg tgagcaccac gtggagtacg ccacgagcga
     2581 gatgcaccgc ttcatcgact tcctcgagcg caccaccgga aggaagctcg acatggagag
     2641 gctccgtgag gtcgttgccc tgtcggacga ggcctgcagg ctctgggacg agatcatgtc
     2701 gtaccggagg ttctctccgg cgccgatcac ggcctccgag atcggcatca tgttcgtcat
     2761 ggtgaccagg cagggtacca aaaccgccgt ggaattcctc gagagggttc gcgacgaggt
     2821 ggcccagagg gcgcacaagg gcatgggcgc cgtggaagac gaacgcgtga ggctcttttg
     2881 ggacaacatc cccctgtggc acaacatggg tatctttaac tacttcgaga gctcgggagg
     2941 ggtgttcgtg gccgagacct actcggctgc ctggtctctc aggctcgacc ccgacgaccc
     3001 ggtgaggtcc ctagcgctca agagcctcat gtcgtacccc ctcgtgtcct gtgtctccat
     3061 cgaccgccgg aaggagatgg tcctgaaggc atgccgcgac tacgccatcg acggggcggt
     3121 gttccacagg aacaggtcgt gcgtgcccat ctcg
//
LOCUS       DAOSCR010000315         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19773_length_3151_cov_3.607558, whole genome shotgun sequence.
ACCESSION   DAOSCR010000315 DAOSCR010000000
VERSION     DAOSCR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19773_length_3151_cov_3.607558"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLT69_08165"
     CDS             <1..334
                     /locus_tag="PLT69_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ14449.1"
                     /translation="GDPFFSTDTNAALRALEVGADVLVKATKVDGIYDRDPVKDPGAV
                     FFRELDYDEVLAGRLAVMDATAVSLCRENGLELRVINIFDPLSLKRLVAGEEVGTTVR
                     GRRKDRGG"
     gene            324..881
                     /gene="frr"
                     /locus_tag="PLT69_08170"
     CDS             324..881
                     /gene="frr"
                     /locus_tag="PLT69_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOJ14450.1"
                     /translation="MVDEIINEMKKDMAKTLDAMKGQLQKVRTGRASIGILDGIMVDY
                     YGTPTPLNQLATLSVPEPRLITIQPWDKGALGPIEKAILKSELGLTPNNDGRIIRVPI
                     PPLNEERRRELVKTIKKSAEDFRIEIRNHRRDANALLKDLEKEKQINKDELKAAQDKV
                     QELTDDYIEKIDELLAAKEKEIMEI"
     gene            971..1468
                     /locus_tag="PLT69_08175"
     CDS             971..1468
                     /locus_tag="PLT69_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14451.1"
                     /translation="MNSGNNGKETSRFMMYFRPGNLSELRVISRMLNIAMVDFVCAAM
                     EAYIHKREGELGSPIPQRNKKRYVGPRNDTSERRIRYVMRLEKDLVEKIRDIACHDNR
                     RFTHVCDEAIANFISFTKAAKNFTNIWTNRPDVEAVRGRKSFRSIEEIDALLDLYKKT
                     PVSSR"
     gene            1483..3003
                     /locus_tag="PLT69_08180"
     CDS             1483..3003
                     /locus_tag="PLT69_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ14452.1"
                     /translation="MACPAVPSTQESAAPAPGPWKERLTLSLGSRGFPLGSGAWHAAS
                     AAGLVSAACVYTLYGLYFVHLHAGFLSREAAFIAVSGMTPLVPPFDAHLAAFPHLLGS
                     ALFFGLTLGLLCGAVCMVLTLPAWFSGRLGPGDLPLFLAASGICTYLGFSLENAAVSV
                     AAGILCPAAFTAAWAFVLAKRCHGPSRTRMWPLFLAVLCAPQLVVLASGASMTHIRDA
                     LVSSPVLSRVSDFYYRHTLLAADVIKPVAARAQNIIAVSADLGPIGPMPHGTLWVRTA
                     DPCGVRAARLVVAPGPLPCPSVVAKGDGRPLNAGDRVLRVLARDADPNRIMRGSIGFF
                     LFSGPLVALFAFVLSLAALCLSRLAARAPATAAALLALYLFACMPAARCAALKVVLEN
                     RPEKVSEYATSGSETRRYLAVTTFPGAVAEETLRRMLTDPSPRVRINALVEAGRRRAR
                     GLLDEVVALTRDPELNVRTKACWALGRIGAGKALDELERIAREDPSGTCATTRTRR"
BASE COUNT          580 a         1010 c         1056 g          505 t
ORIGIN      
        1 gggagacccg ttcttctcca cggacaccaa cgccgcgctc agggcgctcg aggtgggggc
       61 tgacgtcctg gtcaaggcga ccaaggtgga cggcatctat gacagggacc cggtcaagga
      121 ccccggcgcg gtgttcttcc gcgagctcga ctacgacgag gtcctcgcgg gaaggctggc
      181 ggtcatggac gcgaccgccg tctcgctgtg ccgtgaaaac ggtctggaac tcagggtcat
      241 caatatcttc gacccgctct ccttgaaacg gctcgtcgcc ggagaggagg tggggacgac
      301 ggtgaggggg aggaggaagg accgtggtgg atgagatcat caatgagatg aagaaagaca
      361 tggccaagac ccttgacgcc atgaagggcc agctccagaa ggtgaggacc ggcagggcct
      421 ccatcggcat cctcgacggc atcatggtgg actactacgg gacgccgacg ccgctcaacc
      481 agctcgcgac cctgtcggtc cccgagccga ggctcataac catccagccg tgggacaagg
      541 gtgcgctggg ccccatcgag aaggccatac tcaagtcgga gctcgggctc acccccaaca
      601 acgacggcag gatcatccgc gtcccgatcc ctcccctgaa cgaggagcgg aggcgggaac
      661 tcgtcaagac gatcaagaag agcgcggaag acttccgcat agagatccgc aaccacagga
      721 gggatgcgaa cgccctcctg aaggacctcg agaaggaaaa acagatcaac aaggacgagc
      781 tcaaggcggc ccaggacaag gtgcaggagc tgaccgacga ctacatcgag aagatcgacg
      841 agctccttgc ggcgaaggaa aaggagatca tggagatctg agcctgcagg aacccgaggg
      901 cggaccaacc ctgcaggctg cacgaaggaa cgggcgcggc gtgaatccgg cttgagcgag
      961 gaggacacca gtgaacagcg ggaacaacgg caaggagaca tcgcgtttca tgatgtattt
     1021 caggccgggc aacctgagcg agctcagggt gatttcgcgc atgctcaaca tagcgatggt
     1081 cgatttcgtg tgcgccgcca tggaggcgta catccacaag agggaagggg agctcggctc
     1141 ccccataccc cagaggaaca agaagagata cgtggggccc cgcaacgaca cctcggaaag
     1201 gcgcatccgc tacgtcatgc gcctcgagaa ggatctcgtg gagaagatca gggacatagc
     1261 ctgccacgac aaccggaggt tcacccacgt gtgcgacgag gcgatcgcga acttcatctc
     1321 gttcaccaag gccgcgaaga acttcaccaa catctggacc aaccggccgg acgtggaggc
     1381 ggtccgggga aggaagagct tcaggtccat cgaggagata gacgcactcc tggatctcta
     1441 caagaagaca cccgtctcct cccgctgagc gaggggcatc cggtggcctg tcctgcggta
     1501 ccctccaccc aggaaagcgc ggccccggcc cccgggccat ggaaggaaag gctgaccctc
     1561 agcctgggaa gccggggctt cccgctcggc tcgggtgcgt ggcatgccgc ttccgcagcc
     1621 gggctcgtct ctgcggcctg tgtgtatacc ctctacgggc tgtacttcgt gcacctgcac
     1681 gccggcttcc tgtccaggga ggcggccttc atcgcggtct cgggcatgac gcccttggtc
     1741 ccgcccttcg acgcacacct cgcggcgttt ccccacctgc tcggaagcgc cctgttcttc
     1801 gggctcaccc tcggtcttct gtgcggggcg gtgtgcatgg tcctgaccct gcccgcctgg
     1861 ttctccgggc gcctgggccc gggcgacctg ccgctctttc tcgccgcctc gggaatatgc
     1921 acctacctcg gcttcagcct cgagaacgcg gccgtctccg tcgccgcagg catcctgtgc
     1981 ccggcggcat ttaccgccgc ctgggcgttc gttctcgcga agagatgcca cgggccctcc
     2041 cgcacgcgga tgtggccgct gttccttgcg gttctctgcg ccccccagct cgttgtcctg
     2101 gcctccggcg cctccatgac gcacatccgt gacgcgctgg tctcgagccc ggtgctctcg
     2161 cgggtgagcg acttctacta ccggcacacc ctcctcgcag cggacgtcat aaagcctgtg
     2221 gccgcgaggg cccagaacat catcgccgtc tcggcggacc tcgggccgat cgggccgatg
     2281 ccgcacggta ccctctgggt gaggaccgcc gatccgtgcg gggtaagggc cgcaaggctc
     2341 gtggtcgcac ccgggccctt gccgtgcccc tcggttgtcg caaagggtga cggaaggccc
     2401 ctgaatgcgg gcgacagggt gctgagggtg ctcgcgaggg atgcggaccc gaacaggatc
     2461 atgaggggat cgatcggttt cttcctgttc tccgggccgc tcgtcgccct gttcgcgttc
     2521 gtgctctccc tggccgccct gtgtctctcg cggctggctg cacgggctcc ggcgaccgcc
     2581 gcggcgctcc tcgctctcta cctcttcgcc tgcatgccgg ccgcgaggtg cgccgcgctc
     2641 aaggtcgtgc tcgagaacag gccggaaaag gtctccgagt atgcaacgtc cgggagcgaa
     2701 acgaggcgtt acctcgcggt gacgacgttt cccggggccg tggcggaaga gacgctgaga
     2761 cggatgctca cggaccccag cccccgcgtc aggatcaacg ccctcgtgga agcaggccgc
     2821 aggcgggccc ggggcctcct cgacgaggtc gtcgccctga cgcgcgaccc ggagctcaac
     2881 gtgaggacca aggcgtgctg ggcgctcggc aggatcggtg ccgggaaggc cctcgacgag
     2941 ctcgagagga tcgcgaggga agacccgagt ggtacgtgcg cgactacgcg tacgcggcga
     3001 taggcctcct gaagcccgag gcgagggaga tcctgcttac aggcgggatg gagatcggcc
     3061 gccggtgaac ggggggatgg ggcggtcacg gtcactgggc cgatgcgtgg gggtatgcct
     3121 cgatgttgag atcgttcacg ttctgtggta g
//
LOCUS       DAOSCR010000316         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19790_length_3148_cov_4.064662, whole genome shotgun sequence.
ACCESSION   DAOSCR010000316 DAOSCR010000000
VERSION     DAOSCR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19790_length_3148_cov_4.064662"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLT69_08185"
     CDS             complement(<1..251)
                     /locus_tag="PLT69_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14453.1"
                     /translation="MTGDVLPGGAVVEFRVPPLGVEVEAIGGRYVFVRQGVLGRDEGE
                     ILYLVGCAVTDRSCCGQAGCAFVLVAGFLRSRLDAHDERG"
     gene            672..1712
                     /locus_tag="PLT69_08190"
     CDS             672..1712
                     /locus_tag="PLT69_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14454.1"
                     /translation="MSLFDKLFGRSKLSKEISACESALEKRPNDPVILKKLGDLYLKA
                     EDMKNASEVYVRLGEVYTTRGFYPKAVALYKQAIKINASWEEPYVKLAELYQMQGFIR
                     EAATQYMRLSEMLAKSGDSLEATMYMQKAAELDPAITQVSRQVHSFKVKEKAMVEPVP
                     GSSPRPQVERTDFYDLTKELEGHFDSALEAAAQDHPTDGGEVEDTTGVESVFKAIEEN
                     AAREGKDDPLFLYNMGLAYRETGLLEDAADSFRKVLLLGEKLLDAHIMLGITYRELGL
                     FSESLDTLKQSLALEEASQEMKMGILYEIAQTYKAMGDNENALSIFREIQKDHRDFKD
                     VELEIIRLAGGG"
     gene            1714..2295
                     /locus_tag="PLT69_08195"
                     /pseudo
     CDS             1714..2295
                     /locus_tag="PLT69_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
     gene            2914..>3148
                     /locus_tag="PLT69_08200"
     CDS             2914..>3148
                     /locus_tag="PLT69_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14455.1"
                     /translation="MQADRFLTGFTPDVTILGTGLSWPVVNAVLGNCYLFVIVVSLLC
                     FVIVGVFCLVKYAPLMLHDIKKITNNELPQAGLV"
BASE COUNT          661 a         1021 c          945 g          521 t
ORIGIN      
        1 ccgcgctcat cgtgcgcatc aagcctcgat ctcaagaagc ctgcaaccag gacgaaggcg
       61 catccagcct gaccgcagca ggacctgtcc gtgaccgcgc aaccgacgag gtagaggatc
      121 tccccctcgt cgcgacccag gacaccctgt ctgacgaaca cgtaccggcc gcctatggcc
      181 tccacctcaa cgccgagcgg gggcaccctg aactccacca ccgcacctcc cggaaggaca
      241 tcgcctgtca tgcgcattcc tctcccctta tgccccaacg cctcgtgctc atgcaagggg
      301 caggacctcg cagcgggccg tcgcctttcc catgcccggg tgtaccgccc gggtggacgc
      361 ggaggtgccg gcaaagtgtg cggcccgagc gctcgtgtcc ctcgaccggt ccggtatcga
      421 gccagggtcc cgtgcggttc acgaacgggg tcggtcgtgc gctttctgca ggggtcaacc
      481 cgcctgtgtg gttcatccgc cacccaaaca gcccttcatc gggggcgtca cggagaggac
      541 gacccccctt ttccttcttg taatccctta tcctcccaac taaagtttgc atactgtaac
      601 accgatgagc catatacgca ctcggggtgt cgctgagtgc gtacggtcct cggagagggt
      661 tgagagagaa tatgagcctg ttcgataagc tcttcggccg gtccaagctg tcgaaggaga
      721 tctccgcctg cgagtcggcc ctggagaaga gaccaaacga cccggtcatc ctcaagaagc
      781 tgggggatct ctacctcaag gccgaagaca tgaagaacgc atccgaggtg tacgtgcgcc
      841 tgggggaagt ctacaccacc agggggttct accccaaggc ggtggccctg tacaagcagg
      901 ccatcaagat caacgcctcg tgggaagagc cgtacgtgaa gctcgccgag ctctaccaga
      961 tgcaggggtt catccgcgag gcggccaccc agtacatgag gctctccgag atgctcgcca
     1021 agagcgggga cagcctggag gccaccatgt acatgcagaa ggccgcggag ctggacccgg
     1081 ccataaccca ggtcagcagg caggtccact ccttcaaggt caaggaaaag gccatggtcg
     1141 agccggtgcc cggctcttcc ccgagaccac aggtcgagcg gacggacttc tatgacctca
     1201 ccaaggaact cgagggtcat ttcgactccg ccctggaagc ggcagcccaa gatcatccca
     1261 ccgacggcgg ggaggtcgag gacaccacgg gcgtcgagtc ggtgttcaag gccatcgagg
     1321 agaacgccgc cagggaaggc aaggacgacc ccctgttcct gtacaacatg ggcctcgcgt
     1381 acagggagac aggcctcctc gaggatgcgg cggactcgtt caggaaggtg ctgctcttgg
     1441 gggagaagct cctggacgca cacatcatgc tcggcatcac ctacagggag ttggggctct
     1501 tcagcgaatc gctggatacg ctcaagcaaa gcctcgccct cgaagaggcc tcccaggaga
     1561 tgaagatggg gatcctctac gagatcgcac agacatacaa ggccatgggc gacaacgaaa
     1621 acgctctgtc catattccgc gagatccaga aggatcatcg ggatttcaag gacgtggagc
     1681 tcgagatcat caggctggcc gggggaggat gacatggcgg tgatcaacta ttccaagaag
     1741 gagatctctg cgaagatcgt ttattacgga ccctccctgt gtggaaagac caccaacatc
     1801 cagtcgatct acacgagcat caagcccgag cagaagggga agctcgtctc gctggccacc
     1861 gaggcggacc gcaccctgtt cttcgacttc cttcccatag agatcgcgaa catccgggga
     1921 ttcaagacga gactccactt ctacacggtc cccggccagg tctactacaa ctccacgcgg
     1981 agggcggtcc tgacgggcgt ggacggcatc gtcttcgtcg ccgactccca gcgggacaag
     2041 atggaggaga acgtcgagag cctcaacaac ctggaggaga acctcaacta ctacggcaag
     2101 agcatcaaga ccctgcccat ggtgttccag tacaacaaga gagacctccc cgacgtctcg
     2161 tcggtcgccg agctcgagca gatcctcaac ccctacgggt accccttctt cgaggcggtc
     2221 gcgacgacag gcgagggggt cctcgagacc ctcacgagga taaccaagct ggtgctgcag
     2281 cacatcgaga tggggacgag cgggaagcag cagaggcctt cgtccgtgcc cttcgggacg
     2341 gccccacggg gcatccccga gccccccgac gtcttccagc agagacccgc gcctgccggg
     2401 ccgggtcctg tcgagccccc gcggcctcgc gacctcgctg gtcctgaacc gttcgagccc
     2461 ggccgtgcat tccaggccga gaagccttcc cgcccgcttg ctctgggacc ggaggacggc
     2521 tcgatcgcag ctcgcacgac ccaggagaca agcgcggctg tgcggcccgt cggccaggcc
     2581 accccctcag acctcctctc ggaggcgggg ccttctgcaa cctacggaga acttgccgag
     2641 cccgccggtg tcgagcggtc agggtaccct cgcgagacag gcgggctgga acccggtctc
     2701 gccgcgaaac cgctcgagac cttccaggac gacaccgccc gcgtcatgga acaggcgctc
     2761 ggcaggatcc agatcgtggg cacggaaaat gcagagattc tgtccgccaa cgcgctctgc
     2821 ataccgatca aggtgaagat agagggggat cagaaggtgt acaggctgga tttggccatc
     2881 aagatagacg gcctcaagcc gtagggaggc agattgcagg cggatcgctt cctgaccggc
     2941 ttcaccccgg acgtgacgat cctcggcacg ggcctgtcat ggcccgtggt gaacgccgtc
     3001 ctgggaaact gttacctttt cgtgatcgtc gtgtcccttt tgtgctttgt gatcgtgggt
     3061 gtcttctgcc tggtcaagta cgcccccctc atgctccacg acatcaaaaa aatcaccaac
     3121 aacgaactcc cacaggccgg gctcgtcg
//
LOCUS       DAOSCR010000317         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_19955_length_3130_cov_2.778537, whole genome shotgun sequence.
ACCESSION   DAOSCR010000317 DAOSCR010000000
VERSION     DAOSCR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19955_length_3130_cov_2.778537"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1084)
                     /locus_tag="PLT69_08205"
     CDS             complement(98..1084)
                     /locus_tag="PLT69_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HOJ14456.1"
                     /translation="MEERKYDVILATLPATKTRLEVLFRQAGDGFLQVEYGRVQRANL
                     LDSFRIQTIDAKIRQAAPKGFIETLPGIRTNMIHFDPEQLDVMKLIDMIKGFEEELED
                     VDHIVMPSRVIHLPIAFEDSETKKAVEKYVKEVRPDAPNVINGYNLEYIALCNGITVP
                     EVKQKLLGTQWYNSGGGFWPGGAFLWPMDPRCAIVVPKYNPPRTWTPEGTVGIGGPCV
                     FTYTTPTGGGYQLFGRTIPVFQFAQKHPLFKDSPFLYRNGDRVQFHEVTEKEVLDIFE
                     HVHNKTDYVYQIEDGEIVVKDYIAFINSPEVVKGTAEFQAKQAEGIKKAPRL"
     gene            complement(1188..1940)
                     /locus_tag="PLT69_08210"
     CDS             complement(1188..1940)
                     /locus_tag="PLT69_08210"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOJ14457.1"
                     /translation="MKVDLNVDMGESFGMYTMGDDENFMPYITSANVACGFHAGDPCV
                     MKKTVALAKKYGVQVGAHPGLPDLQGFGRRVMEVSMEELYCDLMYQVGALKAFLDAAG
                     MRLHHVKPHGKLYGMAHTREEVAKTICEAIRDIDASLYLYCMKKGVLGQVAQSYGIRP
                     VFEVYSDLDYDKDGNLVITRHHEAHSPEDVAAKVIGMVRDGKVKTTSGTEIAIEGSSV
                     CVHSDSPGAIEIVKAVRKVLTDNGYTIESPGI"
     gene            2790..>3130
                     /locus_tag="PLT69_08215"
     CDS             2790..>3130
                     /locus_tag="PLT69_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14458.1"
                     /translation="MSDATYAMQNAPEAPGLIPLQAEAQPNPRAPEGEDGGRDTDLAR
                     RVRLALAGQGVKAPVIHVRETLSTNSLAHSLARLGHPEGTVVIADSQTAGRGQFGRSW
                     HSAEGKGLYLSV"
BASE COUNT          513 a         1019 c          916 g          682 t
ORIGIN      
        1 cttgctcaag taaccgagcc ttccgggcca gtcttccacg atggtttcta tgccgggctt
       61 tatgatcttc aacatatgtg ctgtcctcct tgtgctctta gagacgaggg gccttcttga
      121 taccctcggc ctgcttggcc tggaactcgg ctgtgccctt gaccacctcg ggtgagttga
      181 tgaaggcgat gtagtccttg accacgatct cgccgtcctc gatctggtag acgtagtccg
      241 tcttgttgtg gacgtgctcg aagatgtcga ggacctcctt ctccgtcacc tcgtggaact
      301 ggacgcggtc gccgttcctg tagaggaagg gcgagtcctt gaagaggggg tgtttctggg
      361 cgaactggaa taccggaatg gtgcggccga agagctggta acctcccccc gtgggggtgg
      421 tgtacgtgaa gacgcagggc ccgccgatgc ccacggtgcc ttccggggtc cacgtacgcg
      481 gcgggttgta tttggggacg acgatcgcgc atctcgggtc catgggccag aggaaggcgc
      541 cgcccggcca gaacccgcct ccggagttgt accactgcgt acccaagagc ttctgcttca
      601 cctcggggac ggttatcccg ttgcacaggg cgatgtattc gagattgtag ccgttgatca
      661 cgttcggggc gtcgggccgt acttctttca cgtacttctc gacagccttc ttggtctcgc
      721 tgtcctcgaa cgcgatgggg aggtggatga ccctcgacgg catgacgatg tggtcaacgt
      781 cctccagctc ctcctcgaac cccttgatca tgtcgatcag cttcatgacg tcgagctgct
      841 cgggatcgaa gtggatcatg ttcgtcctta tcccgggcag ggtttcgatg aaccccttgg
      901 gggccgcctg cctgatcttc gcgtcgatcg tctggatcct gaaggagtcg agcaggttgg
      961 ccctctggac cctcccgtac tcgacctgta ggaatccatc ccctgcctgg cggaacagca
     1021 cctcgaggcg cgtcttcgtc gcaggcaacg tcgccagaat gacgtcgtac tttcgttctt
     1081 ccatcctcta tcctcccttc gtttgatcga ttgaccggac ctcttctctt ctttccctac
     1141 ctcaaggtca gcccaccctg cccggggttc atgacgacgg gcgcgtgcta gatcccgggc
     1201 gactcgatgg tgtaaccgtt gtccgtgagg accttcctga ccgctttcac gatctcgatc
     1261 gcgcccgggc tgtccgagtg gacgcagacg gaagacccct cgatcgcgat ctcggtgccc
     1321 gaggtggtct tgaccttgcc gtctctcacc atcccgatga cctttgcggc gacgtcctca
     1381 gggctgtgcg cctcgtgatg cctggtgatg acgaggttgc cgtccttgtc gtaatcgagg
     1441 tcggagtaca cctcgaacac cggcctgatg ccgtagctct gtgcgacctg gccgaggacg
     1501 cccttcttca tgcagtagag gtacaggctt gcatcgatat ccctgatggc ctcgcagatc
     1561 gtcttggcga cctcttctct cgtgtgcgcc atcccataga gcttgccgtg cggcttcacg
     1621 tgatggagcc tcatcccggc tgcgtccagg aaggccttga gcgcgcccac ctggtacatg
     1681 aggtcgcagt agagctcctc catggacact tccatgaccc tcctcccgaa tccctggagg
     1741 tccggaagcc ccggatgagc acccacctgt acgccgtact tcttcgcgag ggccacggtc
     1801 ttcttcatca cgcacgggtc acccgcatgg aatccgcagg cgacgttggc gctcgtgatg
     1861 tagggcatga agttctcgtc gtcgcccatg gtgtacatcc cgaagctctc gcccatgtcc
     1921 acattgagat ccaccttcat actcccctcc ttgtctggcc tctgcacaag acccttcttc
     1981 cccccgcacg aggtgcgcgg gcagtctacc ctgtatcgtc caccggggct gcgtctttgg
     2041 ccgggctcgg gtgcacctgg atgaggccga gaccgaggcg ttccgcgggc cctcggtcgg
     2101 caggcaagga tccggcaccg gacgtgcccg cgtcgctgcc ggcttgggtc cacatcctgc
     2161 ggtatttttc cggccggatg cgctcgtcat gcaccaagat catcgatgaa gaccccatcc
     2221 ggccgaaagg caccctcgag gaagacggct tcccgcgccc gcaccgctca cgtctcgcgt
     2281 tgcggaccgc atccacctct tctctcgaac agacagacat cctcataccc cgcggaaaca
     2341 ccctggcacc ccccggcggc cctggactga cccgattcga gatcgttcat ctctcagcaa
     2401 agaaagtgcc atggtcgcca tggacgcctg cgggctcgcg atgcaaggca catccatgcg
     2461 cgcctggtcg cctcgaggag tgtcgagcac gaaggatccg ttgcacgatt gcatcagaat
     2521 gccatggtgg gttcctgttg cgttgcattg tcaatgcgat agatcatcgg tgcattgttg
     2581 caatgggatg catggttgca acaggtgaat caaggttatc attcaggtct tcaccccctt
     2641 ctcatgcctt gccgtttcct gtcaagatcc ctgggagccc atctgtgggg cgatcacccg
     2701 tgtcccagcc cttgtccatg tcactttctc agacctggcc cggaccactc ggcacggcgg
     2761 catctttctt gcttgcgtga gatcgcatca tgagcgacgc gacgtatgcc atgcagaacg
     2821 cacctgaggc ccccgggctc atcccccttc aggccgaggc gcagccgaac ccgcgtgcac
     2881 ccgaggggga ggacggcggc agggacacgg acctcgcccg gagggtgagg ctcgcccttg
     2941 cggggcaggg agtgaaagcc cctgtcatcc acgtccggga gaccttgtcg accaacagcc
     3001 tggcccacag cctcgcacgc ctgggtcacc cggaagggac ggtcgtcatc gccgactccc
     3061 agaccgcggg ccgcggtcag ttcggtcgct cgtggcactc ggccgaaggc aaggggctct
     3121 acctctcggt
//
LOCUS       DAOSCR010000318         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20056_length_3118_cov_4.280770, whole genome shotgun sequence.
ACCESSION   DAOSCR010000318 DAOSCR010000000
VERSION     DAOSCR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20056_length_3118_cov_4.280770"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(621..1538)
                     /locus_tag="PLT69_08220"
     CDS             complement(621..1538)
                     /locus_tag="PLT69_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14459.1"
                     /translation="MKHRTLRLLVFVACVLASAYVFQGCSTGGDTEEEEPIETPDLSG
                     VWWGLMRSELVFGVVVPLEGDDTGLEYRTMLVSDASAYISPVEDVYDAFPYDALPLRV
                     VQENAAVIGGDLVQYEGGSLAYAAAVEEKLWLNGVGYEKSLLGGPLLGNGAVRFYRKD
                     VDGELESGRVSQFVLYYNDAFEDAPNVRDLQGTWTLSGGWAFGNDMELTITPYVTTTT
                     GGTVSGRDARGNTISGTIVIHATEPASSVYDLDLSLNGVPMSGLAAKIVFSESDENKP
                     SGMSEGVYLVLCAASADFSRILGGLAEPE"
     gene            2002..2562
                     /locus_tag="PLT69_08225"
     CDS             2002..2562
                     /locus_tag="PLT69_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14460.1"
                     /translation="MSNTTRTPKKTRMIFPAFIAAALTLMATACSAVVTTFTDPSTSA
                     EVIRMSGNRLAGGFKTLELNAQRFLKEGNPSFSLFVVYRGPSFMEVAAGQSLVLEIDG
                     RRVELNGRGSKDHRVPISIGLVEETSYYHDVAPDLLRDLASSTEVTVVLRGDSGTLKR
                     RFSKKNFEAFRRFWEQAGSGLDPAGK"
BASE COUNT          574 a         1055 c          931 g          558 t
ORIGIN      
        1 tgccgacgca gtgggcgacg taccccagcc ccgtggctgc ttcctttttt atatcttctc
       61 aaggcccctc gcccggctga ctggccctct ccctgcggtc accctgtacg accgggtccc
      121 cggacatgcc ccaggcgggc ctacccgaac ctttaagtat acacactggg gttcgccgcc
      181 gagaccctgg gcatcatatc caccgggcgg gccttcccgt gccccatcct cttgagttta
      241 cacaccaggt agaccatcct cccagacctc tcccacgggg gcatttctct cgagcgagaa
      301 gggggcatta ttgaagagtc gggacatccg actgacatac ctcacccttt cgccggcatc
      361 ctggatcacc gagcgccccc atgcacagga cgggtctcga gaggcggacc aaagtctcct
      421 ggaagagtca ctcgtcagac agcagggtcc cagcccgcat ccaggtccct cgacatcggc
      481 gattacgcga aaagcctttt caggtcgggc cattgcccgc gtgcggatcc cacgaggagg
      541 tatcctgtgc ggtcacccca cccccaggga acgcgtgcac gagagggtac gatcgtcctg
      601 ggtggtcaca ggccccaccc tcactcaggc tctgccagcc ctcccagtat cctggagaag
      661 tcggcgctcg ccgcgcacaa gacgaggtat accccctcgc tcatcccgga gggtttgttc
      721 tcgtctgact cgctgaacac gatcttggct gcgagcccgc tcatggggac gccgttcaag
      781 gaaagatcca ggtcgtagac gctcgaggcc ggctccgtgg cgtggatcac gatggtgccg
      841 cttatggtgt tgccccttgc atccctgccc gagacggtgc cccccgttgt cgtggtcacg
      901 tagggggtga tggtgagctc catgtcgttg ccgaatgccc agccgccgga gagagtccag
      961 gtcccctgga ggtccctgac gttcggcgcg tcctcgaagg cgtcgttgta atacaggacg
     1021 aactgggaga ccctgccgct ctcgagctcc ccatccacgt ctttcctgta gaacctgaca
     1081 gcgccgtttc cgagcagagg accgcccaag aggctcttct cgtagcccac gccgttgagc
     1141 cacagcttct cctccacggc cgcggcatag gcgagcgagc cgccttcgta ctgcacgagg
     1201 tcgcccccga tgaccgcagc gttctcctgg accaccctca agggcaaggc gtcatagggg
     1261 aaggcgtcgt acacgtcctc cacgggcgag atgtaggcgc tggcgtccga gacgagcatg
     1321 gtccggtact ccaggccggt gtcgtcgcct tcgagcggga cgacgacccc gaacacgagt
     1381 tcgctcctca tcaaacccca ccacaccccg gagagatccg gggtctctat gggctcctct
     1441 tcctctgtgt ccccgcccgt ggagcatccc tggaacacgt aggcggatgc gagcacgcat
     1501 gcgacgaaca cgaggagacg cagtgtcctg tgcttcatgt ccttccctcc ttgcatcgtc
     1561 cttcaccgtt caatcgacca gtacgagatc aaggataacg atgatgccgg gaaaagacaa
     1621 cccgccttcg tgcgcgaggt gagcccgctc tccgtcaccc gttccctgtg ctcgcccgcc
     1681 ccgtcgatac caccgacctg ccgtgcgtgc aaccagcccg cgaaagggcg atccgtgaaa
     1741 aagccttcga gcctccgcct gactcggtca ttccaggcgc aacccactgc atgaacgaga
     1801 agagaggaaa cgcttccctg gcgcatgtcg ttacgtgaac agtggccagg tagggggagc
     1861 gcgatcccct gcataaaaac agtgcagcgg ggtgtgcccc gctgtcacgc ccgggcatcg
     1921 agtttgcata caagcatggc agcccgcaaa gggcgggggt cggccgagcg caacgaatac
     1981 gttcatgcag ggagacacac catgagcaac acgacacgga caccgaagaa gacccggatg
     2041 atctttccag cctttatagc cgcagccctc accctgatgg cgaccgcctg ctcggccgtc
     2101 gtcaccacct tcacggaccc ttccacctcg gccgaggtca tccgcatgag cggcaaccgg
     2161 ctcgcaggcg ggttcaagac cctggagctc aacgcccagc gctttctcaa ggaagggaac
     2221 ccctcgttct ccttgttcgt cgtgtatcgc gggccgtcgt tcatggaggt ggctgctggg
     2281 cagagccttg ttctcgagat cgacgggagg agggtcgagc tgaacgggag aggctcgaaa
     2341 gatcacaggg tccccatctc catagggctc gtggaggaga cctcctatta ccacgacgtg
     2401 gccccggatc tgctgcgcga tctggcttcc tccacggagg tgaccgtggt tctccggggt
     2461 gattccggca cgctgaagcg ccgcttctcg aagaagaact tcgaggcgtt caggcgcttc
     2521 tgggagcagg cgggctccgg cctggacccg gccgggaagt gacaagacgc caagggcggc
     2581 cacaggctcg accttgcgaa ccggcctgcg agatgcgcac gatgcgacat ctccgggcca
     2641 aggcacgcgg gcgtggatac gcacgagagc ggagctgcgc ccgatccttc ggatttctcc
     2701 ccgaggtact tgttcaacca gggctccgct cgctcttctt gccgctgtct tcgtccctcc
     2761 ccggtctcaa ggcgtcacct ccttcctctg gaatgacgga aaagcacagg gctcgacgcg
     2821 ttacggcccc ccggtcgggc ctgaggcgaa agcacgccat ggaaaccgta ctaggtatgg
     2881 catgcctcgt tcggcgtcct ctcaaaggat catcacgccg cctacgcatc gcccgttcgg
     2941 gaagcacctc ctcaggccct gccgggtccg ggagggtgtc gatgccgtgc cagcgggagc
     3001 cgttgatgga accctaaccg cacaggagag ggaggtcgat gccgtgcctg cggggacagc
     3061 ccgggttctt cgagggtgac cgcacaggcc ctgcggaaga aacagcagcc cctcgatc
//
LOCUS       DAOSCR010000319         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20175_length_3105_cov_2.962295, whole genome shotgun sequence.
ACCESSION   DAOSCR010000319 DAOSCR010000000
VERSION     DAOSCR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20175_length_3105_cov_2.962295"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..696)
                     /locus_tag="PLT69_08230"
     CDS             complement(130..696)
                     /locus_tag="PLT69_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ14461.1"
                     /translation="MEFKDLIRARRSIREFDHKAVPMDVLRLIVADSTMAPNARNAQP
                     WAFSIVANRDLIRRLSDESKKNILWDMDENPSSPYKTYRAILEDAGFNVFYNAPCVVF
                     ISGSKAVRSIFVDCALAGAYFMLSAADRGLGTCWVDLGSAIRDKGLRGELGLPDDHVI
                     VAAIAVGYPASVPAPPPRSDPRILRVVE"
     gene            1165..2979
                     /locus_tag="PLT69_08235"
     CDS             1165..2979
                     /locus_tag="PLT69_08235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ14462.1"
                     /translation="MWISTYQGNAAVEAAVKQFLPPCQERCPINEDIQRTNVLVSMLP
                     RDPERAREGIVQIGDYLFERNPFFPVCGYVCGVCELGCNYKTKGGSIRRRLIKRFIAE
                     NYLSHLDSREEYDVVKGKGNVAVVGGGPAGLMCAFELGRRGYRVTVFEALNRLGGALW
                     LIPHYRLPKDVLHRVIQNMVRMASIEVKLGVRIGEAKKGLDWLRKQGYQAVFIAKGTP
                     QPRILTFGKDPVENQDLSGVMYGQSFLYEVSRGILPGDYFDGRKVIVVGGGNVAFDVA
                     RSARRLGGDVTVVALESEDKKSKDGLPADEEEIRGAWEEGIDLMCSRGVSQIIGRGGK
                     FTGIKMPLCTCVFDKDGRFNPTFDCSDVMTMEGDVLIITVGQGPDRSLLEREGLIDET
                     GRLAVDPVTRQSTIKDWVFVGGDVRRVGYMVDAVKDGMEAAESIERHLKGVDLTTGRK
                     RVFEALDIPQLKDDSYKPEPEVVWIPPEKRMHFRMFERGLTLEEAVEEARRCLCCGPC
                     VSCKACVSIGIQKDLPAVIVSERLCSGCGICISSCHYGAAYLKRDNGDIVSCTDTYRC
                     KACGMCVSACPAQARELTGTDMNDRISQVFAGLEGGAR"
BASE COUNT          585 a          969 c         1003 g          548 t
ORIGIN      
        1 aagacacgcc gcccttcaga cggggaggcc ggcggccctt gttggaacat cacctctgca
       61 gcccaccgag cattcccatc cgctggaggc tccccgagcc acgaaggccg accggggtga
      121 accccggccc tactccacca ccctcaggat cctggggtca gacctcggcg gcggcgcggg
      181 gaccgaggcc gggtatccca cggcgatcgc ggccacgatc acgtggtcgt ccggaagtcc
      241 gagttcgccc ctcaggccct tgtccctgat ggcgctcccc aggtccaccc agcaggtgcc
      301 gagccccctg tccgcggccg agagcatgaa gtacgcccct gcgagcgcgc agtccacgaa
      361 gatggacctg acggccttgc ttcccgagat gaacaccacg cagggggcgt tgtagaacac
      421 gttgaatccg gcatcctcga ggatcgccct gtacgttttg tacggagagg acgggttctc
      481 gtccatgtcc cagagtatgt tcttcttgct ctcgtccgac aggcgcctga tgaggtccct
      541 gttcgcgacg atgctgaagg cccacggctg agcgtttctc gcgttgggcg ccatggtgct
      601 gtccgcgacg atgagcctca ggacatccat gggcaccgcc ttgtgatcga actccctgat
      661 ggaacgcctc gctctgatga gatccttgaa ttccatcgaa aaccctcctt tgtgacattt
      721 tagacgactc gcactccccg ggggcttctc ttcacaccga cccgcgcacc tcctgcagcc
      781 agggtatgcc tccaggcggt ctttgggaag ggggggagcg tggtccgact cgcaaccccg
      841 gcaatcaacc cggcaccggg ttcacccccg ccgttcacgc tctccattct tcgtcttccc
      901 ttgccgcacc ggccacccga acccctgcct tcattggagg acatagtatc atctgttcct
      961 cgaaggcgcc cacgccgcag tggggcaatg ggccccacca tggggtactt ttcccctctc
     1021 tcctcgggcc ggttccttgg tgcctcaccc ttgcgactca ataatatcgg aatgttgcat
     1081 gacgtggcac cgttgttgct gatgggtgcg caacgaccgg gcggcctgcc caccggtcta
     1141 ctttaactga gaggttctcg tgccatgtgg atttcaacct accaggggaa cgcagcagtg
     1201 gaagcggctg ttaagcagtt tctgccgccg tgccaggaac gctgtccgat caacgaggac
     1261 atccagagga ccaacgtcct cgtctccatg ctgccccgtg acccggagag ggcgagggaa
     1321 gggatcgtcc agatcgggga ctatctcttc gagcgcaacc ccttcttccc ggtgtgcggg
     1381 tatgtgtgcg gcgtgtgcga gctcggctgc aactacaaga ccaagggcgg ttccatcagg
     1441 agaaggctga tcaagcgttt catcgcggaa aactacctct cccacctcga cagccgggaa
     1501 gagtacgacg tggtgaaggg gaagggaaac gtggcggtgg tgggaggcgg ccctgccggg
     1561 ctcatgtgcg ccttcgagct gggcaggagg gggtacaggg tcaccgtgtt cgaggcgttg
     1621 aaccgcctcg gcggtgcgct gtggctcata cctcactatc gtctccccaa ggacgtgctc
     1681 cacagggtga tccagaacat ggtcaggatg gcctccatcg aggtcaagct cggcgtgcgc
     1741 ataggcgagg cgaagaaagg cctcgactgg ttgcgcaagc agggctacca ggccgtgttc
     1801 atcgcgaagg gcacccccca gccgaggata ctgaccttcg gcaaggaccc cgtcgagaac
     1861 caggacctct cgggtgtcat gtacggccag tcgttcctgt acgaggtgag ccgcggcatc
     1921 cttccgggcg actacttcga cgggagaaag gtgatcgtgg tgggcggcgg caacgtcgcc
     1981 ttcgacgtgg ccaggtccgc gcgcaggctc ggcggagatg tcaccgtggt cgccctcgag
     2041 agcgaggaca agaagagcaa ggacgggctg ccggcggacg aggaggagat caggggcgcg
     2101 tgggaagagg gcatcgacct catgtgttcc cggggcgtga gccagatcat aggcaggggc
     2161 gggaagttca cgggcatcaa gatgcccctg tgcacgtgcg tgttcgacaa ggacggccgg
     2221 ttcaacccaa ccttcgactg cagcgacgtc atgaccatgg aaggcgacgt gctcatcatc
     2281 accgtcggcc agggaccgga caggtcactg ctcgaacgcg agggcctgat cgacgagaca
     2341 gggcggctcg cggtcgatcc ggtcacccgc cagagcacca tcaaggactg ggtcttcgtg
     2401 ggaggggacg tgcgcagggt cgggtacatg gtggacgccg tcaaggacgg catggaagcg
     2461 gccgaatcca tcgaacgcca cctcaagggc gtcgacctga ccaccggcag gaagagggta
     2521 ttcgaggctc tcgacatccc gcagctcaaa gacgacagct acaagccgga acccgaggtc
     2581 gtgtggatac cgcccgagaa acgcatgcat ttcaggatgt tcgagcgcgg cctcacgctc
     2641 gaggaggccg tcgaggaagc gcgcaggtgc ctgtgctgcg ggccgtgcgt gtcatgcaag
     2701 gcgtgcgtgt ccatcggcat ccagaaggat ctgcccgccg tgatcgtcag cgagaggctc
     2761 tgcagcgggt gcgggatctg catctcctcg tgccactacg gggcggccta cctgaagagg
     2821 gataacggcg atatcgtctc gtgcaccgac acgtaccggt gcaaggcctg cggcatgtgc
     2881 gtctcggcat gcccggctca ggcgagggaa ctgacaggga cagacatgaa cgaccgcata
     2941 agccaggtgt tcgcaggtct ggaaggaggt gcgagatgag cgtgaaggcg accggcccgc
     3001 ttcctttctc ccccaagctc gtgtgcttca cctgcaggtt cgggtgggga tacctgaacg
     3061 gatccaaggc catcgagagc ctccacaacg tcatacccgt cacct
//
LOCUS       DAOSCR010000320         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20321_length_3089_cov_3.443309, whole genome shotgun sequence.
ACCESSION   DAOSCR010000320 DAOSCR010000000
VERSION     DAOSCR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20321_length_3089_cov_3.443309"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLT69_08240"
     CDS             complement(<1..635)
                     /locus_tag="PLT69_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ14463.1"
                     /translation="MKKTLTAKIIESHLAGSSADEYLLTIDQTLTQDATGTMAYLEFE
                     AMGIDRVKTGLSVSYVDHNTLQTGFENADDHRFLQDAAAKFGVLFSRPGNGICHQVHL
                     ERFGAPGKTLLGSDSHTPTAGGIAMLAIGAGGLDVAAAMAGMPFALPRPKVVGVILEN
                     SLKPWVSAKDIILELLRRLTVKGGVGKIFEYLGPGVETLSVKEIIEYGQVTA"
     gene            1050..2603
                     /locus_tag="PLT69_08245"
     CDS             1050..2603
                     /locus_tag="PLT69_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ14464.1"
                     /translation="MLAGKRRVMGEVLVELGLVDEYRLRHALEVSEREGIRLGESLIR
                     LGYLDEDHVLEILRDLTGVHTLNMRAWAVKKHAQTLMSRERMAELKAVPLDANSKRAV
                     VAFADPFDYLAVEKVKFLINREVTPVLATHSQIEDILAHLEAVGYGRKTVQLSEVKRG
                     NANVTLQDMDVSVMLKLLDDPECTSVHLSPGASPALRVGGSFKRCRLPIVNSQVLERI
                     VGELTDEELRERLRDRKEIEFTYSKPGMMRYRVNIYRQKGSELGVMIKKLREDIPTPS
                     SLGLPRVLLDMLDKKGLVLISSARGQGKDATIAALVGHLNSTRSLNIVTFEDPLEYIH
                     RNKFSNVNQREIGSDTGEDVQDVFDRALRLDPDVLVVTELKGAFMMDTATVAAHKGIL
                     VIAGISAVDVFSALEQFVSSLSSDYMRGLFSRSLVGAYSQRLLRHGTRKKAALAWECI
                     LASPRIQKYISDDKIYFIKGQAPALKGEYMPVEESVAQAIKAGALDYESVRNEHWINQ
                     EALAAILGR"
     gene            2763..>3089
                     /gene="atpE"
                     /locus_tag="PLT69_08250"
     CDS             2763..>3089
                     /gene="atpE"
                     /locus_tag="PLT69_08250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOJ14465.1"
                     /translation="MRRVATFVLVTVMLAVSGLAFAADGAQAVYVDWAKIIFVAASIL
                     AAGLCMGIGATGAGSGMGQALSGATEAVGRNPDAQGKIMLTLMVGLAMIESIAIYALV
                     ISLIILY"
BASE COUNT          583 a          893 c         1031 g          582 t
ORIGIN      
        1 gccgtgacct ggccgtattc gatgatttcc ttgacgctca gggtctcgac gcctggtccc
       61 aggtattcga agatcttccc cacgcctccc ttgaccgtga gcctcctcaa cagctcgagg
      121 atgatgtcct tcgcgctcac ccagggcttt aggctgttct cgaggatgac gcccaccacc
      181 ttaggccttg gcagggcgaa gggcatgccc gccatggcgg cggccacgtc gagcccgccg
      241 gcgccgatgg cgagcatggc gatcccgccg gcggtgggcg tgtggctgtc ggagccgagc
      301 agcgtcttgc ccggcgcccc gaagcgctcg aggtgcacct ggtggcagat gccgttgccg
      361 ggcctggaga acaggacacc gaacttggcc gccgcgtcct ggagaaaacg gtggtcgtcg
      421 gcgttctcga aaccggtctg gagcgtgttg tggtccacgt agctgaccga gagcccggtc
      481 ttcaccctgt cgatgcccat ggcctcgaac tccaggtagg ccatggtccc ggtggcgtcc
      541 tgcgtcaggg tctggtcgat ggtcaggagg tactcgtcgg cgctggatcc ggccaggtgg
      601 ctctcgatga tcttggcggt gagggtcttc ttcacgggca tggcctccgt cgagtgtcac
      661 ctttctctaa tggatggcat cgaccccgtc aagggtcgcc ggggtttttc acggctgccg
      721 tcatgaaaag ggcaaacagg cccctgtccg ccacccggca ttgaccgggg cgggtctgtc
      781 ccgggcgcac cgggggggct gccccacctc ctcgccaggc ccgcggcggc tcgtgtcacg
      841 accgatccgg gccgggagac cggcacgcgg cgtcatcgaa agggttcgct cggattctgg
      901 tggccatggc gccgacgatg cccggcgctc gatacccacg cggttgcagg gagatggatg
      961 tcgcaggggt gcgttgttga caaaaggata catcttccga aaagagatac tgtctcgttt
     1021 ggagacggac accgacagtg gagggttcga tgctggcagg aaagcggcgt gtcatgggag
     1081 aggtgctcgt ggaactcggc ctcgtggacg aatatcgcct gaggcatgcc ttggaggtat
     1141 ccgagaggga agggatacgg ctcggggagt ctctcatccg gctcggctac ctcgacgagg
     1201 atcacgtcct ggagatcctc agggacctga caggtgtgca cacgctcaac atgagggcat
     1261 gggcggtgaa gaagcacgcc cagacgctca tgtccaggga acgcatggcc gagctcaagg
     1321 ccgtccccct cgacgccaac agcaagaggg ccgtggtggc gtttgccgac cccttcgact
     1381 acctggcggt ggaaaaggtc aagtttctca tcaacaggga agtgacaccg gtgctggcga
     1441 cgcattccca gatcgaggat atcctcgcgc acctcgaagc cgtgggctac ggaaggaaga
     1501 cggtccagct gagcgaggtg aagaggggca acgcgaacgt gaccctgcag gacatggacg
     1561 tctcggtcat gctgaagttg ctcgacgatc ccgagtgcac gagcgtccac ttgtcgccgg
     1621 gtgcctcgcc ggcactccgc gtcggcggct ccttcaagag gtgcaggctt cccatcgtga
     1681 actcgcaggt cctggagcgg atagtcggag agttgacgga cgaggaactg agggagcgcc
     1741 tgcgtgaccg caaggagatc gagttcacct actcaaagcc cgggatgatg cgataccggg
     1801 tgaacatcta tcggcagaag gggtccgaac tgggcgtcat gatcaagaag ctcagggagg
     1861 atatcccgac cccgtcgagt ctcgggctcc ccagggtgct cctggacatg ctggacaaga
     1921 aggggctcgt actcatcagc agcgcgcgcg ggcagggcaa ggacgccacg atcgcggcgc
     1981 tggtaggcca tctcaacagc acgaggagcc tgaacatagt caccttcgag gatccgctcg
     2041 agtacattca ccgcaacaag ttctccaacg tcaaccagcg ggagatcggc agcgataccg
     2101 gcgaggatgt gcaggacgtg ttcgatcgcg ccctcaggct cgatccggat gtgctcgtcg
     2161 tcacggagtt gaagggggcg ttcatgatgg ataccgccac ggtggccgcg cacaagggga
     2221 tactcgtgat cgccgggata agcgccgtgg acgtgttctc ggcgctcgag cagttcgtct
     2281 cctcgctgtc gagcgattac atgagggggt tgttctcgag gtcgctcgtg ggggcgtact
     2341 cgcaaaggct cctgcggcac ggtacgcgaa agaaggccgc gctcgcgtgg gagtgcatcc
     2401 tcgcgtcgcc gaggatacag aagtatataa gcgacgacaa gatatacttc atcaagggcc
     2461 aagcgcccgc gctcaagggc gagtacatgc ccgtcgagga gagcgtggca caggcgatca
     2521 aggccggggc gctcgattac gagtcggtga gaaacgagca ctggatcaac caggaggcgc
     2581 tcgcggcgat ccttggtcgg tgatgcgggc aggaggaagg ggctcgccgc attttttatg
     2641 aacgagtaat cattcattct cactttgggc ttgcatggcc cttcctgatg gtgtatggta
     2701 atgaaccgtg attcagaaca atcctctgaa ttcatgaata aaaaacatct aaggagtgtg
     2761 cgatgagaag agttgcgacc tttgtccttg ttaccgtgat gctcgccgtt tctggtctcg
     2821 cgttcgcagc tgatggggcg caggcggtat acgtcgattg ggccaagatc attttcgtcg
     2881 ccgcgtcgat cctcgcagcc ggtctttgca tgggtatcgg agcgacaggc gccggttcgg
     2941 gcatgggcca ggccctctcc ggggcgacgg aagcggtggg ccgcaacccc gacgcccagg
     3001 gcaagatcat gctcacgctc atggtcggcc tcgcgatgat cgagtccatc gcgatctacg
     3061 ccctcgttat ttcgctcatc atcctgtac
//
LOCUS       DAOSCR010000321         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20363_length_3085_cov_3.503960, whole genome shotgun sequence.
ACCESSION   DAOSCR010000321 DAOSCR010000000
VERSION     DAOSCR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20363_length_3085_cov_3.503960"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..820)
                     /locus_tag="PLT69_08255"
     CDS             complement(5..820)
                     /locus_tag="PLT69_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ14466.1"
                     /translation="MVVLCLVFSVLTPWVEPGAHAAKPGVITVATTGTCPPMEMKGPK
                     GRVVGYEVDLMEAVAKEAGLKVKFVERPWSKLLDELERGRCDAVIAQIAVTDARKQRF
                     DFSEPYLTGRKVLVVRSEGAAEPLEGKSLGVFRLCPKAEALRLYQRCSIAYYTMAETD
                     RAFVDLADGFIAGVLAEAPVASHYVLDDKRFKGRFRVADVPAFGGCQAPEEEYAIAVR
                     KGDTKLLGLLNKGLGRLKEKGVLEKIFARWVKELPVRGGTEGVAGLKQPATQQ"
     gene            1100..2077
                     /locus_tag="PLT69_08260"
     CDS             1100..2077
                     /locus_tag="PLT69_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ14467.1"
                     /translation="MDILDLVMKWCAENGRMGGVAWLSRREFMRRQGLLAASLAFSGL
                     APKVSWAASDRPYLSVVTGAPGPAARMAIELLGGMGSFVKRGQKVVIKPNMSFSGGVR
                     DGVTTHPEVVREIVAMCSECRAGRILVLDHTLRPPERCIGEIRNACRPFGGDIVHGLD
                     SRDFYRQAAIPQGVDLKGAEVMKDVLDADVLIAVPVAKSHSSTGVSLSMKGMMGLVWN
                     RTVMHWRHDLDESIVDLCTLLKPSLVVIDATRVLSTNGPSGPGEVIFADTVIASRDMV
                     AADAYTVAAFPWYGRRLPPEKVRHVRIASARGLGRMDVGNLEVKKVTLR"
     gene            2074..>3085
                     /locus_tag="PLT69_08265"
     CDS             2074..>3085
                     /locus_tag="PLT69_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ14468.1"
                     /translation="MRPKRLAQAASLAIFSALVVCASESLVCGVAARWFLGMDPAGVV
                     QVASAERDIPAGYVPALCVLALTLLAGRFFCSMVCPLGALVDVTDSVFGAQGRVARPK
                     TAGMRGGKYLVLGILAASAAFGLALAAFLSPLSIMARLVGLVLHPLGMDAAGAFVRAA
                     RPLAEDLGMTRIAYAAVTPRDYGLEALSAGVAGIALAGVVLGPRFWCRCLCPAGAVFA
                     LFSVRPLLVRRRVNEGCTSCMRCVERCPMLAIGPDPRKTDHAECIACMRCARVCPVSA
                     VGFLPGGGRTQGRGAGFSRSRRFVLLGVLFAAASGAVLARFLNPVGLGGSRVARAGFG
                     VLR"
BASE COUNT          449 a          990 c         1076 g          570 t
ORIGIN      
        1 cgcctcattg ctgcgttgcg ggctgcttga ggcccgccac cccttccgtc cctccccgca
       61 cagggagttc cttcacccac ctggcaaaga tcttttcgag gacgcccttt tccttgagcc
      121 ttcccagtcc cttgttgagg agcccgagga gcttcgtgtc gcccttcctc accgcgatgg
      181 cgtactcctc ttcaggggcc tggcagccgc caaaggcggg gacgtcggcc accctgaacc
      241 tgcccttgaa cctcttgtcg tcgaggacgt agtgggatgc gaccggcgcc tcggccagga
      301 cgcccgctat gaatccgtct gcgaggtcca cgaacgcccg gtctgtctcg gccatggtgt
      361 agtaggcgat ggagcacctc tggtagagtc tcaacgcctc tgccttcggg cacagcctga
      421 acacgcccag cgatttcccc tccagaggtt cggccgcgcc ctcagagcgc accacgagca
      481 ccttgcggcc cgtgaggtag ggttccgaga aatcgaagcg ctgcttcctg gcgtccgtga
      541 cggcgatctg tgcgatcacc gcgtcgcacc tgccccgttc gagctcgtcg aggagtttcg
      601 accatggcct ttccacgaat ttcaccttca ggcccgcctc cttggcgacg gcctccatga
      661 gatccacctc gtagcccacc accctgccct tggggccctt catctccatg ggcggacacg
      721 tccccgtggt tgcgacggtg atcaccccgg gcttcgccgc gtgtgcgccc ggctcaaccc
      781 aaggcgtcag cacggagaac acgaggcaga ggacgaccac ggcggtggac accccctgtc
      841 ttgaggcgtc ccgatcgcgt gactcgatgc gcgagcatgg cttcatggat gccggatggt
      901 accaggaacg agggcgcagg gcaacagggt tgcacagggg cccggtcact gcagggccac
      961 ttcggggtgc gtgagaaggg ggccgatcat ggatggagct gcatgcaggc gggcttgggg
     1021 ccgcgcgcgc ttcccttcgg ggcccgctcg ggctatgata ggtcttgagg gaggcctctg
     1081 tcgttgccgg gagggcgtca tggacatcct cgatctcgtg atgaagtggt gtgcagaaaa
     1141 cggccgcatg ggcggggtgg cctggttgag caggcgcgag ttcatgaggc gccagggcct
     1201 gctcgcggca tccctggcct tttccggcct cgccccgaag gtgtcgtggg ccgcgtccga
     1261 caggccgtat ctcagcgtcg tcacgggcgc acccgggccg gccgcgagga tggccatcga
     1321 gctcttgggc ggcatgggct ctttcgtgaa gagggggcag aaggttgtca tcaagccgaa
     1381 catgagcttt tccggggggg tgagggacgg cgtcaccacg caccccgagg tggtcaggga
     1441 gatcgtggcc atgtgcagcg aatgccgggc aggccgtatc ctcgtcctgg accacacgct
     1501 caggccgccc gaacgctgca tcggcgagat aaggaacgcg tgcaggccct tcggcgggga
     1561 catcgtccac ggcctcgact cgagggactt ctatcgccag gccgccatac cgcaaggcgt
     1621 cgacctgaag ggcgccgagg tgatgaagga cgttctcgac gccgacgtcc tcatcgcggt
     1681 ccccgtggcc aagtcgcact cgagcaccgg cgtgagcctt tccatgaagg ggatgatggg
     1741 gctcgtgtgg aaccggaccg tcatgcactg gcgtcacgac ctcgacgagt cgatcgtgga
     1801 cttgtgcacg ctcttgaaac cctcgctggt cgtgatcgac gcgaccaggg tcctttccac
     1861 gaacgggccg tccggtccgg gcgaggtgat cttcgcggac accgtgatcg catccaggga
     1921 catggtcgcg gcggacgcct acaccgttgc agccttcccc tggtatggga ggaggctccc
     1981 cccggagaag gtcaggcacg tgaggatcgc atccgccagg ggactggggc ggatggacgt
     2041 ggggaacctg gaggtgaaga aggtgacgtt gcggtgagac cgaaacgcct tgcccaggca
     2101 gcctcgctcg cgatcttctc ggcgctcgtg gtatgcgcct ccgagtccct tgtgtgcggc
     2161 gtcgccgcac ggtggttcct ggggatggac cctgcgggcg tggtccaggt ggcctccgct
     2221 gaaagggaca ttcccgcagg gtatgtgccc gccctctgcg tgctggccct caccctcctc
     2281 gccggaaggt tcttctgctc catggtatgc cccctcgggg ccttggtcga cgtgacggac
     2341 tcggttttcg gtgcacaagg aagggtggcg aggccaaaga ctgcgggcat gagggggggc
     2401 aagtacctgg tcctcggaat cctcgccgca tcggcggcct tcgggctggc ccttgcggcg
     2461 tttctctccc ccctctccat catggcgaga cttgtgggcc ttgtcctcca ccccctgggg
     2521 atggacgcgg caggcgcctt cgtgagggcg gctcggcccc ttgcggaaga tctcggcatg
     2581 acgcggatag cgtatgcggc ggtgacgccc agggactacg ggctcgaggc cttgagcgcg
     2641 ggcgtcgcgg gcatagcgct cgccggggtg gtcttggggc cccgcttctg gtgcaggtgc
     2701 ctctgccctg ccggagcggt gttcgcgctc ttctcggtga ggcccctctt ggtcaggagg
     2761 agggtgaacg agggatgcac gtcgtgcatg aggtgcgtgg agcgttgccc catgctggcc
     2821 atcggcccgg acccgagaaa gaccgaccac gcggagtgca tcgcctgcat gcgctgcgcg
     2881 agggtgtgcc cggtctcggc ggtcgggttc ctccccggcg gtgggaggac gcagggtcgg
     2941 ggagcgggtt tctcgcgcag ccggaggttc gtgctcctcg gtgtcctgtt cgctgcggca
     3001 tccggcgccg tcctcgcgag gttcctgaac cccgtgggcc tcgggggaag ccgggtcgca
     3061 cgagcgggtt tcggcgtctt gaggc
//
LOCUS       DAOSCR010000322         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20455_length_3074_cov_3.878768, whole genome shotgun sequence.
ACCESSION   DAOSCR010000322 DAOSCR010000000
VERSION     DAOSCR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20455_length_3074_cov_3.878768"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLT69_08270"
     CDS             <1..396
                     /locus_tag="PLT69_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ14469.1"
                     /translation="TENNSCYNTCPVTAIFVVHEVLKWLLDKGGVPEMEKVNEAKARL
                     IYEILDASSFYRAPVEKDSRSRMNIPFKLPTEELDNEFVAQASKKGLVGLKGHRAVGG
                     IRASIYNAMPMEGVERLAEFMREFEKSHS"
     gene            785..1663
                     /locus_tag="PLT69_08275"
     CDS             785..1663
                     /locus_tag="PLT69_08275"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOJ14470.1"
                     /translation="MAWGKGSRPLSILVGLMAIILAGCSMKALTIRSMEPIMRDMDLA
                     VNRSTDVEMLKDALPAFLVQMDGLILASESPSLLLKAAEANFGYANAFLEDTDKPRAS
                     ALYLKARDYAARALYGTGPHEKALEEPLEAFTKTLGRFTRPDVPALFWTANCWLAWGA
                     LNLDRSEAVMDMPKAQAMLERVIELDETFFYGAAHAALGAIHAAKGGLVRGSLERAKE
                     HFDKAFAISQGRLQFIPLYYAQYYAYQTQDRDLFVSTLEGILARRPDAYPDKAFVNEV
                     ARRKAKVLLENVDMYF"
     gene            1685..2752
                     /gene="dctP"
                     /locus_tag="PLT69_08280"
     CDS             1685..2752
                     /gene="dctP"
                     /locus_tag="PLT69_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOJ14471.1"
                     /translation="METRKLFISIVLSLITALISAQQPASAQNAPVVIKMATLAPKGT
                     ALAKAFEEMAQEIQDKTKGEVVFKTYWGGVQGDEKDVMRKIRLGQLHGGGFMGVGLGQ
                     VVPQVRVTEIPYVFRNYDEVAYVRGKLQPEMERLFEERGFKVLGWMDLGFVYIFSKVP
                     LSDLNVARRQKWWALEGEPLSMAMFQALDISPVSLSISDVATSLSTNLIDCANSTPFG
                     AVAFQWYTKFRYMTGYPSTNIQGATIVSKDVWNRISPASQKIVIEVARAKHKKIEQVT
                     RMEDERSLELLKKSGIQIVKYDIKDKEMQYVFEAAKKARESLVGKVYSRELLERTLAY
                     VEEYRRLHPEDTTVIRLDKGN"
     gene            2782..>3074
                     /locus_tag="PLT69_08285"
     CDS             2782..>3074
                     /locus_tag="PLT69_08285"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOJ14472.1"
                     /translation="MDDFPGHRCPACGFTVERPSGLFKAVAYVEDAALTVILSAMVLL
                     VLVQIVLRDVFSTGIAGGSETVRHMVLWTAFIAAGLAGREGKHIRIDIAHRVLP"
BASE COUNT          575 a          988 c         1010 g          501 t
ORIGIN      
        1 accgagaaca actcctgcta caacacctgc cccgtcaccg ccatcttcgt ggtgcacgag
       61 gtgctcaagt ggctcctgga caagggcggg gtcccggaga tggagaaggt caacgaggcg
      121 aaggcgcggc tcatctacga gatcctcgac gcgagctcgt tctaccgggc gcctgtcgag
      181 aaggattcgc gctcgaggat gaacatcccg ttcaagctcc ccaccgagga gctcgacaac
      241 gagttcgtcg cacaggcctc caagaagggg ctcgtggggc tcaagggcca cagggccgtg
      301 ggcgggatca gggcgtccat ctacaacgcc atgcccatgg agggtgtgga gcggctggcg
      361 gagttcatga gggagttcga gaagagccac tcctgatcga cgacgcgaaa gaacgcatcg
      421 ttgcagccgt gggacatgac gggcgggccc tcaccaagag ggaccgcccg tcttcatcca
      481 tgaggagtcc gcggccggag gcgcctgcgg cgggtcgtac ccgccgatcc cggacaacgg
      541 cccgcccccc gtggccaccc gcgccatgcg agcctctgcc cctgcaggcc ttcgcgcgtc
      601 cgaggtcggg gagcaagggc tcgcgatcct catggtcgat ggcgtacacc cgccgccaca
      661 gcctgcagcc ggcttgggga caaagacctt gacacgcatg tccgaatgtc ggattctggt
      721 gagtggtgtg gatcccccct tgccgggcgg gggatcgtat cttctcttca tgacgggggg
      781 ttgtatggcg tgggggaagg gttcacggcc cttgtcgatc ctcgtgggcc tcatggcgat
      841 catcctcgcg gggtgctcca tgaaggcgct caccatacgg tccatggagc cgatcatgcg
      901 ggacatggac ctggcggtca accgctccac ggacgtggag atgctcaagg acgcgctgcc
      961 tgcctttctc gtccagatgg acgggctcat cctggcttcc gagagcccct cgctcctgct
     1021 gaaggccgcc gaggcgaact tcgggtacgc caacgccttc ctcgaggata cggacaagcc
     1081 gcgggcttcc gccctctacc tgaaggcgag ggactacgca gccagggccc tctacggcac
     1141 agggccgcac gaaaaggcgc tcgaggagcc cctggaagcg ttcacgaaga cgctcggcag
     1201 gttcacccgc cccgatgtcc ccgcgctctt ctggaccgcg aactgctggc ttgcctgggg
     1261 cgccctcaac ctcgacagga gcgaggcggt catggacatg cccaaggccc aggcgatgct
     1321 cgagcgggtg atcgagctcg acgagacctt cttctacggc gccgcccacg ctgccctggg
     1381 cgcgatccac gccgccaagg gcggcctcgt gcggggctcg ctcgagaggg ccaaggagca
     1441 cttcgacaag gccttcgcca tctcccaggg ccggctccag ttcatcccgc tctactacgc
     1501 gcagtactac gcctaccaga cccaggacag ggacctgttc gtctccacgc tcgagggcat
     1561 cctcgcgagg cggccggacg cgtaccctga caaggccttc gtcaacgagg tcgccaggcg
     1621 caaggcgaag gtgctgctcg aaaacgtgga catgtacttc tgacaaccgg ggggcacgca
     1681 ctcgatggaa acgagaaagc ttttcatctc gatcgtcctt tcgctcatca cggcgctcat
     1741 ctctgcacaa cagcccgcat ccgcccagaa cgcgcctgtc gtcatcaaga tggcgacgct
     1801 cgcgcccaag ggcactgcgc tcgcgaaggc cttcgaggag atggcgcagg agatccagga
     1861 caagaccaag ggtgaggtgg tcttcaagac ctactggggt ggggtccagg gcgacgagaa
     1921 ggacgtgatg cgcaagatca ggctgggcca gctccacggc ggcgggttca tgggcgtggg
     1981 cctcggccag gtggtgcccc aggtcagggt cacggagatc ccctacgtgt tcaggaacta
     2041 cgacgaggtc gcctacgtgc ggggcaagct gcagcccgag atggagcgcc tcttcgagga
     2101 acgcgggttc aaggtgctgg ggtggatgga cctcggcttc gtctacatct tctccaaggt
     2161 gcccttgagc gacctgaacg tggcgcgcag gcagaagtgg tgggccctgg agggcgagcc
     2221 cctcagcatg gccatgttcc aggccctgga catctccccc gtgtccctgt ccatctccga
     2281 cgtggcgacc tccctgtcca ccaacctcat agactgcgcc aactcgaccc cgttcggcgc
     2341 cgtggccttc cagtggtaca ccaagttcag gtacatgacc ggctacccgt ccacgaacat
     2401 ccagggggcc accatcgtga gcaaggacgt gtggaacagg atctccccgg cctcgcagaa
     2461 gatcgtgatc gaggtcgcca gggcaaagca caagaagatc gagcaggtca cccgcatgga
     2521 agacgagcgc tccctggagc tcctcaagaa gtccggcatc cagatcgtga agtacgacat
     2581 caaggacaag gagatgcagt acgtcttcga ggcggcgaag aaggcgaggg agagcctggt
     2641 gggcaaggtc tactcgaggg aactgttgga gaggacgctt gcctacgtgg aggaataccg
     2701 gaggctccac cccgaggaca ccacggtcat caggctcgac aagggcaact gaacatgcgg
     2761 tgccccgcct gcggatccag catggacgat ttccccggcc acaggtgccc ggcctgcggg
     2821 ttcaccgtgg agcggccctc cgggctgttc aaggcggtgg cctacgtcga ggacgcggcg
     2881 ctcaccgtga tcctgtccgc catggtcctg ctcgtgctcg tccagatcgt gttgagggac
     2941 gtgttctcga ccgggatcgc gggcggctcc gagacggtca ggcacatggt gctctggact
     3001 gccttcatcg cggcagggct cgcgggccgc gagggcaagc acatcaggat cgacatcgcc
     3061 caccgggtgt tgcc
//
LOCUS       DAOSCR010000323         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20483_length_3071_cov_2.893236, whole genome shotgun sequence.
ACCESSION   DAOSCR010000323 DAOSCR010000000
VERSION     DAOSCR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20483_length_3071_cov_2.893236"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLT69_08290"
     CDS             <1..341
                     /locus_tag="PLT69_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOJ14473.1"
                     /translation="FEVSADKKTATVTREIEGGQMVVEVPLPAVFSAQKGLNEPRYAS
                     LPGIMKAKKKPVDVKNAGALGVSAGPMTVIKEMSLPPARQAGKIITGDDAKAKAAALA
                     KALHEEVKII"
     gene            360..1328
                     /locus_tag="PLT69_08295"
     CDS             360..1328
                     /locus_tag="PLT69_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOJ14474.1"
                     /translation="MAGTLIYAEVQKGEIKKGAFEITSKAKEFGGEVAACIIGKGAEA
                     LAPQLAKYGADKIYVVDGPEFENYVPEAFVQAFVQVANTVQPTVILTSASAQGKDFTP
                     AVAARLNVGAVSDCVELKKEGDKVIAKRPMYAGKAYALVEAVGTPQVISVRPNALGFK
                     EIAGAGAVEKVAVNLDASKIKVQVKAVEVAATTEPDQTEAEIIVSGGRGMKGPENFKM
                     LEDLAKLFGPTATTGASRAAVDAGWRAHSYQVGQTGKTVSPNLYIACGISGAIQHLAG
                     MGTSKVIVAINKDPEAPIFTKADYGVVDDLFNVVPALIEEIKKIKG"
     gene            1411..>3071
                     /locus_tag="PLT69_08300"
     CDS             1411..>3071
                     /locus_tag="PLT69_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOJ14475.1"
                     /translation="MHGPQGEVTREVFWNILSPGSYTEQILLYILALAAIGALVRALY
                     KGGFGTRLKVIFKAQGTDVDRLDNWKSRLWYAIVDVFAHRKILREPYQGIFHFFIFYG
                     FVALTITTAIVFFQADILYPITGIWFMKGGFYLALSLFADVFGLLCIVGVVMALYRRY
                     SIKPAWLDEKPEDNFTLWFILAILLTGFIVEGLRIHATETNPAHPVHPYIWTSPGGYA
                     IGFVFSWMDERSMRGLHYFMWWIHVLMALTFFVYIGYTKLMHIFMTPVNIFLRPTTPQ
                     PPIKVMPPEMFECAETFGIHKVEEYSWKDLFDQEACMRCGRCVEVCPAFNTDKPLKPR
                     DVIQNIRTHMEQKAKFALDANGVYHILPDESYTGPSLIGDCIEKETAWACTTCMACVE
                     ACPAYILQFPKLIDLRRYLVMMESDFAPELQGVFKGMENNSNPWGIGAHLRADWAKGH
                     DVPLMSEKGSAEYLFYVGCAGAFDDLNKRAAVALAKIFNEAGLDYAILGTEEGCCGDS
                     AKKIGNEYIFQALATANIETFKGYGVKKIITLCPHGYTALKYDYK"
BASE COUNT          629 a          938 c          966 g          538 t
ORIGIN      
        1 agttcgaggt gtcggccgac aagaagacgg cgaccgtgac gagggagatc gagggcggcc
       61 agatggtggt cgaggtgccg ctgccggcgg tgttctcggc gcagaagggt ctcaacgagc
      121 ccaggtacgc ctctctgccc ggcatcatga aggccaagaa gaagcccgtc gacgtcaaga
      181 acgccggcgc tctcggcgtg agcgcgggtc ccatgaccgt gatcaaggag atgtccttgc
      241 ctcctgcccg ccaggccggc aagatcatca ccggcgacga tgcgaaggcc aaggcggcgg
      301 cgcttgcgaa ggccttgcac gaagaagtga agatcatcta aggtggggag ggacgagcca
      361 tggcaggaac actcatttac gcggaagtac agaagggcga gatcaagaag ggcgccttcg
      421 agataacgag caaggccaag gagttcggcg gcgaggtggc cgcgtgcatc atcggcaagg
      481 gggcggaggc cctggcgcct cagctcgcga agtacggcgc cgacaagatc tacgtggtgg
      541 acggccccga gttcgagaac tacgtccccg aggcgttcgt ccaggccttc gttcaggtgg
      601 ccaataccgt gcagcccacg gtcatcctca ccagcgcctc cgcgcagggc aaggacttca
      661 ccccggccgt tgcggcccgc ctcaacgtcg gcgccgtctc ggactgcgtg gaactgaaga
      721 aggaaggcga caaggtcatc gccaagaggc ccatgtatgc tggcaaggcc tatgcccttg
      781 tcgaggccgt gggcactccc caggtcatct cggtcaggcc caacgccctc ggtttcaagg
      841 agatcgcagg cgccggcgcg gtggagaagg tggcggtgaa cctcgatgcc tccaagatca
      901 aggtccaggt caaggcggtc gaggtggcgg cgaccaccga gcccgaccag accgaggccg
      961 agatcatcgt ctccggcggc aggggcatga agggacccga gaacttcaag atgctcgaag
     1021 acctcgcgaa gctcttcgga cccacggcca ccacgggcgc atccagggcg gcggtggacg
     1081 ccggctggag agcccattcg taccaggtcg gccagaccgg taagaccgtg agcccgaatc
     1141 tctacatcgc ctgcggcatc tccggcgcca tccagcacct cgctggcatg ggcacttcca
     1201 aggtcatcgt ggcgatcaac aaggatcccg aggcccccat cttcaccaag gcggactacg
     1261 gggttgtcga cgatctcttc aacgtcgtcc cggcgctcat agaggagatc aagaagatca
     1321 agggttagct gatggaaggt ctccgggggc gggtcgaaca ggtccgcccc cggagtacgt
     1381 gaaccatatc aaacgattct ggggggggct atgcacggtc cacaaggaga agtaacccgt
     1441 gaagtctttt ggaatatcct gagcccgggg tcatacaccg agcagatcct gctctacatc
     1501 ctcgcgctgg cggcgatcgg ggcgctggtc agggcgctgt acaagggcgg gttcggaacg
     1561 aggctcaagg tgatcttcaa ggcccaggga acggatgtcg acaggctcga caactggaaa
     1621 tcgaggctgt ggtacgcgat cgtcgacgtc ttcgcgcacc gcaagatcct cagggaacct
     1681 taccagggaa tctttcactt cttcatcttc tatggtttcg tggcgctcac catcaccacg
     1741 gccatcgttt tcttccaggc ggatatcctc tacccgatca cgggcatatg gttcatgaag
     1801 ggcgggttct accttgccct ctcgctcttc gcggacgtgt tcggcctcct gtgcatcgtg
     1861 ggcgtcgtca tggccctgta ccggaggtac tcgatcaagc cggcctggct cgacgagaag
     1921 cccgaggaca acttcaccct gtggttcatc ctggccatcc tcctgaccgg gttcatcgtc
     1981 gaggggttga ggatccatgc caccgagacc aaccccgccc acccggtgca tccctacatc
     2041 tggacatctc cgggcgggta tgcgatcggt ttcgtcttct cctggatgga cgagaggtcc
     2101 atgcgcggtc tgcactactt catgtggtgg atccatgtgc tcatggcgct cacgttcttc
     2161 gtgtacatcg gctacacgaa gctcatgcac atcttcatga cccccgtgaa catcttcctg
     2221 agacccacca ccccgcagcc cccgatcaag gtgatgcctc cggagatgtt cgagtgcgcg
     2281 gagaccttcg gcatccacaa ggtcgaggaa tactcgtgga aggacctctt cgaccaggag
     2341 gcctgcatgc gttgcggcag gtgcgtcgag gtctgccccg ccttcaacac ggacaagcct
     2401 ctcaagccca gggacgtgat ccagaacatc aggacccaca tggagcagaa ggcgaagttc
     2461 gccctggacg cgaacggcgt gtaccatatc ctgcccgacg agtcgtacac cggtccgtct
     2521 ctcatcggcg actgcatcga gaaggagacc gcgtgggcct gtacgacctg catggcctgc
     2581 gtcgaggcct gccccgctta tatcctgcaa ttcccgaagc tcatcgatct caggcgctac
     2641 ctcgtcatga tggagtcgga tttcgcacca gaactccagg gcgtgttcaa gggcatggag
     2701 aacaactcca acccgtgggg tatcggcgca cacctgaggg cggactgggc caaggggcac
     2761 gacgtgccgt tgatgtccga gaaggggagc gcggagtacc tcttctacgt aggctgcgcc
     2821 ggtgcgttcg acgatctcaa caagagggcc gcagtggccc tggccaagat cttcaacgag
     2881 gcggggctcg actacgcgat cctcggcacc gaggagggtt gttgcggcga ctcggccaag
     2941 aagatcggga acgagtacat cttccaggcg ctggccacgg caaacatcga gaccttcaag
     3001 ggctacggcg tgaagaagat catcacgctc tgcccccacg gctataccgc gttgaagtac
     3061 gactacaagg a
//
LOCUS       DAOSCR010000324         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20652_length_3052_cov_3.130464, whole genome shotgun sequence.
ACCESSION   DAOSCR010000324 DAOSCR010000000
VERSION     DAOSCR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20652_length_3052_cov_3.130464"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLT69_08305"
     CDS             complement(<1..804)
                     /locus_tag="PLT69_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14476.1"
                     /translation="MDMSLNLLIALVVFAVFLLAFWFLNRRSEKIDTGEISSLRELAD
                     KGDAYAQFRLGQIYYEGTAVPRDDREAAAWFSKAASQDHAEAQFILATMYEKGHGVLK
                     NDREAFAWYSRAASLGHERSRVILESGRWDAFGAGPEAARVDDLAEEGALTVGRGEEG
                     QPHDRDKRELFEKYLAKAEAGDANAQYNVGVMYYHGEGVEKDFEKALKWFLLSAQQGD
                     ADAQYSLGFMYGRGEGTVKDRAASLEWFQKAASSGHKGALELLEKMRLRS"
     gene            complement(934..1383)
                     /locus_tag="PLT69_08310"
     CDS             complement(934..1383)
                     /locus_tag="PLT69_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ14477.1"
                     /translation="MSDGLDVVIVDDEYHVCGVLAETIGKFYTWGEIVTFQDYAAATE
                     YCLGRDKGLMVFVLDVFVGQSNGFAFLDSISEKYPNAAMDTVMISGMASDDVVDMCVA
                     TGVSHLLEKPVRPYALQLAIRSIVMKYIKFAKRLLSDSELAGIIDRI"
     gene            complement(1847..2623)
                     /locus_tag="PLT69_08315"
     CDS             complement(1847..2623)
                     /locus_tag="PLT69_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOJ14478.1"
                     /translation="MKTTLGQYLPRDSLVHALDARVKLVVMGLSIGVSFRLDRAGTLV
                     LFALGLVFCTFISKISFGDLYRGLKPFIWLFAATALLHVFMTGGDPLPLVPFATRQGL
                     EGGIRVGAQLVLALWVSTLTTLTTSSLDMVWAMQWFMRPLKYLNVPTEEIALAVMLAI
                     RFIPLLFEEADRITRAQKARGVDLESGNVFRRTRAMVSVVVPLMHGAFRRAEDLAVAL
                     TLRGYRPGIVRSRMREPRVSLKDGVCTALVCLWYGFLLAV"
     gene            complement(2643..>3052)
                     /locus_tag="PLT69_08320"
     CDS             complement(2643..>3052)
                     /locus_tag="PLT69_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOJ14479.1"
                     /translation="RHTLEERAIEAEIRKILKADTMSLSVTCMQVPVFYAHAASVVVE
                     TDGEMDPQTLKKVLAHQQGLELRDDPGQDVYPTQVDALGLDECLVGRIRRDDAFDHGV
                     AFFTACDNVRKGSAVNAVQIAELALAGRSAKRD"
BASE COUNT          534 a         1041 c          914 g          563 t
ORIGIN      
        1 cgacctcaag cgcatcttct cgagcaactc cagggcgccc ttgtgtcccg aggacgccgc
       61 cttctggaac cactcgagag aggcggccct gtccttgacg gtcccttccc ccctgccgta
      121 catgaacccc aggctgtact gggcgtccgc gtccccctgc tgtgcagaga ggaggaacca
      181 cttgagcgcc ttctcgaagt ccttttccac gccctcgccg tgatagtaca tgacgcccac
      241 gttgtactgg gcgtttgcat ccccggcctc ggccttggcg aggtacttct cgaacagttc
      301 cctcttgtct cggtcatggg gttgcccttc ctctccgcga ccgaccgtca aggcgccctc
      361 ttcggcgagg tcgtcgaccc gggcggcttc tggacctgcc ccgaatgcat cccaccgccc
      421 tgattcgagg atcacgcggg agcgttcgtg ccccagcgat gccgccctcg agtaccaggc
      481 aaaggcctcc ctgtcgttct tgagcacgcc gtgacccttt tcgtacatgg tcgccaggat
      541 gaactgggcc tctgcatggt cctgggatgc cgccttcgag aaccacgccg ccgcctccct
      601 gtcgtccctg ggtactgcgg tcccctcgta gtagatctgg ccaagcctga actgggcgta
      661 tgcgtcgccc ttgtccgcga gttccctgag gctggagatc tcccccgtgt cgatcttttc
      721 cgacctgcgg ttgaggaacc agaaggcgag caggaacacg gcgaacacaa cgagcgcgat
      781 gagaaggttg agggacatgt ccatgggatc gtgctatgcc attttcaccg cgaaatcaac
      841 cacaccctgc ccacgcgagg ctgcaaaggg cgcacgtggg gcgcccgtct ctctccgcgc
      901 acggcattca tcggccgcat gggaacacgg gcttcagata cggtctatga tgcccgcaag
      961 ttcgctgtcc gacagcagcc tctttgcgaa cttgatgtac ttcatcacga tggagcggat
     1021 ggcgagctga agggcgtacg gccttacggg tttctcgagg aggtggctca cgccggtggc
     1081 cacgcacatg tcgaccacgt catcgctggc catgcccgag atcatcacgg tgtccatggc
     1141 cgcgttggga tacttctcgc tgatcgagtc caggaaggcg aacccgttgg actggcccac
     1201 gaagacgtcc agcacgaaaa ccatcagccc cttgtccctc ccgaggcagt actcggtggc
     1261 cgcggcgtag tcctggaacg tgacgatctc cccccaagtg tagaacttgc cgatggtctc
     1321 cgcgagcacc ccgcacacgt ggtattcgtc gtccacgatg acgacatcga gtccgtcaga
     1381 catcctttcc ccccgggatc ggtctatgag caatgacttt gcgacatgag aatctcaagc
     1441 cgacaacgtt atatcccaag acaagcactc ttacaatcgt ttgctgggct cggtccctca
     1501 atcgggctgg acgggacgca acccgccctc cccgtgcgcc cctcgtgcac gggctgcctg
     1561 gggttttggg gatactgacc gggggctccg tggatcgtgt gcccctcgtg tacgggccgc
     1621 ccgttgaact gcgccgccct gaagtcgttg ccgtcagcgt gagcctatcg tgtacgggcg
     1681 accccttgag ggtcgtgggc gcggccccag gtgcgacacg gccgggaaag cccggcggac
     1741 gggcatcccg acaatagcgg gaatcggccg gtagccggcc gacaaaaggg gcggcgcttt
     1801 ccgacaggct cccgcgacct ctcccacgtc ccggcggagg caaggttcag accgccagga
     1861 ggaacccgta ccacaggcac acgagcgccg tgcacacacc gtctttcagg ctcaccctgg
     1921 gctctctcat cctgctccgc acgatgcctg gcctgtaccc cctcagggtc agcgcgaccg
     1981 cgagatcctc ggcgcgcctg aaggcgccgt gcatgagcgg aaccacgacg ctgaccatgg
     2041 ccctggtcct cctgaagacg ttccccgact cgaggtccac gccccgcgcc ttctgggcgc
     2101 gcgtgatcct gtcggcctcc tcgaagagca gggggatgaa cctgatggcg agcatgaccg
     2161 cgagcgctat ctcctcggtg gggacgttca ggtacttgag aggcctcatg aaccactgca
     2221 tggcccacac catgtcgagg gatgacgtgg tcagcgtggt gagcgtggac acccacaggg
     2281 ccagcacgag ctgcgcaccc accctgatcc cgccctcgag cccctgcctc gtggcaaagg
     2341 gcacgagggg cagggggtct ccgcccgtca tgaagacgtg aagcagggcc gtggccgcaa
     2401 acagccagat gaacggcttc aggcccctgt acaggtcgcc gaacgatatc ttcgagatga
     2461 acgtgcagaa cacgagaccc agggcgaaca ggacgagcgt gcccgccctg tccagcctga
     2521 aggagacccc tatgctcagg cccatgacga cgagcttgac gcgagcgtcg agcgcgtgca
     2581 cgagagagtc ccgcggaagg tactgtccca gggtcgtctt catcgccttg ttcgcacgtt
     2641 cttcagtccc tcttggcgga cctcccggca agcgccagct ccgctatctg cacggcgttg
     2701 acggccgaac ccttgcggac gttgtcgcag gcggtgaaga acgccacgcc gtggtcgaac
     2761 gcatcgtccc tcctgattct ccccaccagg cactcgtcca ggccgagcgc gtcgacctgc
     2821 gtgggataca cgtcctgccc cgggtcatcg cgaagctcga ggccctgctg atgagcgagg
     2881 accttcttca aggtctgagg atccatctcc ccatcggtct ccaccaccac gctggccgcg
     2941 tgggcgtaga acaccggcac ctgcatgcag gtcacgctca gggacatggt atccgccttg
     3001 aggatcttgc ggatttccgc ctcgatcgcc ctctcctcga gggtatgcct gt
//
LOCUS       DAOSCR010000325         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20745_length_3043_cov_3.486613, whole genome shotgun sequence.
ACCESSION   DAOSCR010000325 DAOSCR010000000
VERSION     DAOSCR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20745_length_3043_cov_3.486613"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLT69_08325"
     CDS             complement(<1..1120)
                     /locus_tag="PLT69_08325"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOJ14480.1"
                     /translation="MEKMRRACSVFVVTAVLLALAGAREAGAALPADSLWVADVAERV
                     LPSVVNISSVKTVTQQSPLFMDPFFREFFGQAIPKERVERALGSGVIVSGDGYIITNN
                     HVVGGADRVEVRLSDGRVFQATIVGTDPKSDVAVIKINKTGLPAIKIGDSSRLRIGEF
                     VLAVGNPYGLEQTVTLGIVSALGRSGLGITDYENFIQTDAAINPGNSGGALVNMKGEL
                     VGINTAILSRTGGSVGIGFAIPVNLAMSIKKSIEKYGKVLRGWLGVTVQELTPELAQS
                     LGLATVKGALVNDVQKGSPADKSGLKRGDVIVAIDGQPVNNTASMRFLVSEALPGTTV
                     RVKVVREGRETTVPVEVGDLSAAKEPEHKILVTDNKFLG"
     gene            1328..2245
                     /locus_tag="PLT69_08330"
     CDS             1328..2245
                     /locus_tag="PLT69_08330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ14481.1"
                     /translation="MSLFMHLNSTKTYHARFPVDLMTECTVCETLAHPGTPTARALGI
                     TRPYGALVGDFLRSKGLLAPGCRVLELGCGYGSLMEGLLRHHGDVVGRAFMADLSPRL
                     VTRQRERLFGVSPRARHVMADIHELPFRGGAFDLVIVNEVAGDLDTIVGVDASAMPGD
                     LSSIVEKYGLETPTSGPFAFNLGAVRLVEELCVLSGAVFLSEHSSDPVVPASMPYLAT
                     GLAVDSFPRRIPLSGHDEYTVRFSHLEKVARSHGRTVHTGPLIEVLGLEPRPSWRFIF
                     TAKACSTEEQALIGEFLDHVREYRWLLIS"
     gene            2320..>3043
                     /locus_tag="PLT69_08335"
     CDS             2320..>3043
                     /locus_tag="PLT69_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ14482.1"
                     /translation="MTEEHKPQEPGSMAELLEEYDRKPRRTPEIGDRVAARIISIGRE
                     HVFVDTGTKLDGIIDRKELELEDGTLPYQEGDVIDVFVQSKRGQEIRLSRALAGIGGL
                     SLLREAKEKGAPVEGKVLEVVKGGYRVETLGHRAFCPFSQMDLARVEDPSVHVGQTYR
                     FLVSELDEDEPDIVLSRRSLLAREREEAQSTFFASLGEGDILSGTVKKLMPFGAVVEL
                     GPDVEGMVHVSEMSWSRILAPED"
BASE COUNT          535 a         1009 c          950 g          549 t
ORIGIN      
        1 cccctaagaa cttgttgtcc gtgacgagga tcttgtgctc gggttctttt gccgccgaga
       61 ggtctcccac ctcgaccggc accgtggtct ccctgccttc acggaccacc ttcaccctca
      121 ccgtcgttcc gggcagggcc tccgagacga ggaacctcat ggatgcagtg ttgttcacgg
      181 gctggccgtc gatggccacg atcacgtccc cgcgcttgag gcccgacttg tcggccgggg
      241 aacccttctg cacgtcgttg acgagggccc ccttcaccgt cgccaacccg agggactggg
      301 cgagctcggg cgtgagttcc tgcaccgtca cgccgagcca ccccctgagc accttgccat
      361 acttctcgat actcttcttg atgctcatgg cgaggttcac cgggatcgcg aagccgatgc
      421 ccacgctgcc gcccgtacgc gacaggatcg ccgtgttgat gcccacgagc tcgcccttca
      481 tgttcacgag cgcgcctccg gagttgccgg ggttgatggc cgcatcggtc tggatgaagt
      541 tctcgtagtc ggttatgccc agaccggatc tgcccaaggc gctgacgata ccgagggtca
      601 ccgtctgctc gagtccgtag gggttgccga ccgcgagcac gaactcgccg atcctcagtc
      661 tcgacgagtc gccgatcttg atcgcgggca ggccggtctt gtttatcttg atcacggcga
      721 cgtcgctctt ggggtcggtg cccacgatgg tggcctggaa caccctcccg tcggaaaggc
      781 gcacctcgac cctgtcggcc ccgcccacca cgtgattgtt ggtgatgatg tacccgtcgc
      841 cggagacgat gaccccggaa ccgagcgcac gctcgacgcg ttccttgggt atggcctgtc
      901 cgaagaactc cctgaaaaag gggtccatga acaggggtga ctgctgggtc accgtcttga
      961 cggaggagat gttcaccacc gacgggagca ccctctcggc gacatccgcc acccagaggc
     1021 tgtccgccgg gagagcagcc ccggcctcac gcgcgcccgc aagcgccaag agaaccgccg
     1081 tgacgacaaa gacgctgcac gctcttctca tcttttccat gtcccctctc ctttctcagg
     1141 ggctcataac acagaacgag ggttccttca acgaaggcac cctttcccgt cttttaccgt
     1201 cttcgggggc tgaagccggc cgcgcgacgc acacgtcccg ccgcaccaag gcgggcatgc
     1261 ttcgccgcat cggcccgtca ccggggctgc ccccatgagc ttgagcgaac caccgcgatc
     1321 gtggtagatg tccctgttta tgcacctgaa ctcgacgaaa acctatcacg cccgcttccc
     1381 ggtcgacctc atgaccgaat gcacggtatg cgagacgctc gcccacccgg gcacccccac
     1441 ggccagggcc ctggggatca cccggcccta cggcgcgctc gtcggggatt tcctccgctc
     1501 aaagggcttg ttggcgcctg gctgcagggt cctcgagctg gggtgcgggt acggttcgct
     1561 catggaaggg ctgctccggc accatgggga tgtcgtggga cgcgcgttca tggcggacct
     1621 ctcgccccga ctggtaacaa ggcagcgaga gaggctcttc ggtgtctcgc cgagggcgcg
     1681 ccacgtgatg gccgacatac acgagctccc gttcaggggt ggcgccttcg acctggtgat
     1741 cgtcaacgag gtggcggggg acctcgacac catcgtgggt gtcgacgcat ccgcgatgcc
     1801 cggcgacctc tcgtccatcg tcgagaagta cggcctcgag accccaacgt caggcccctt
     1861 cgccttcaac ctcggcgcgg tgaggctcgt ggaagaactc tgcgtgctat ccggcgcggt
     1921 cttcttgagc gagcactcga gcgaccccgt cgtgccggcc tccatgccgt acctcgcaac
     1981 cgggctggcc gtggactcct ttcccaggag gatccccttg tcaggccacg acgagtacac
     2041 ggtgcgcttc tcgcatctcg aaaaggtcgc gcgctcgcac ggcaggacgg tgcacacggg
     2101 ccccctcatc gaggtgctcg ggctcgaacc cagaccatcc tggcgcttca tcttcaccgc
     2161 aaaggcatgc tccaccgagg aacaggcgct cataggagag ttcttggacc acgtgcgcga
     2221 gtacaggtgg ctcctcatct cttgagccaa cttttccgtt tactatccgg ggtaattcaa
     2281 gtatagcctc catctaccgc acgcagagag ggaccatcca tgaccgaaga acacaagcct
     2341 caagaaccgg gatccatggc ggagctgctc gaggagtacg accggaagcc tcggaggaca
     2401 cccgagatcg gggaccgcgt cgcggccagg atcatatcga tcggcaggga gcacgtcttc
     2461 gtggacaccg ggaccaagct ggacggcatc atagaccgca aggagctcga gctcgaggat
     2521 ggaaccctgc cttaccagga aggggatgtc atcgacgtct tcgtccagtc caagaggggg
     2581 caggagatca ggttgagcag ggcgttagcc ggcatcggcg ggctcagcct cctcagggag
     2641 gccaaagaga agggcgcccc ggtggagggg aaggtcctcg aggtcgtcaa gggggggtac
     2701 cgggtcgaga ccctcgggca cagggccttc tgccccttca gccagatgga cctcgcccga
     2761 gtcgaggacc cgtctgtcca cgtcggccag acctaccggt tcctggtctc ggagctcgac
     2821 gaggacgagc ccgacatcgt cctgtccagg agatccctcc tcgcgaggga gcgcgaggag
     2881 gcgcaatcga cgtttttcgc atccctcggc gagggcgata tcctgagcgg aaccgtgaag
     2941 aagctcatgc cgttcggcgc cgtggtggaa ctcggtcccg acgtggaagg gatggtgcac
     3001 gtgtcggaga tgagctggtc tcgcatcctg gcgcccgagg aca
//
LOCUS       DAOSCR010000326         3038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20793_length_3038_cov_3.611130, whole genome shotgun sequence.
ACCESSION   DAOSCR010000326 DAOSCR010000000
VERSION     DAOSCR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20793_length_3038_cov_3.611130"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(875..1327)
                     /locus_tag="PLT69_08340"
     CDS             complement(875..1327)
                     /locus_tag="PLT69_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ14483.1"
                     /translation="MSLTGQKKAERRRHERKRASIPARLKISFSSRDSGKILKGIQNI
                     VALAETRDISIGGLSLKIVATPMEARTSLTPANSAQVVGRPIEVVLEDENVVIWGDVV
                     RTDKNTMELAIVVYKVSDVREWKRICSENREGLSIFPDTPAVRKRRRT"
     gene            1876..2217
                     /locus_tag="PLT69_08345"
     CDS             1876..2217
                     /locus_tag="PLT69_08345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ14484.1"
                     /translation="MTPGDETPERRRHRRKQVHITVLLKVGVLLNGRGVAKDISPGGM
                     RLVSSQIFKATTTIQAKDYEGSTMRVMVPTEGLTVNGVIAWVDLKKGEGALKVQSTSD
                     DAKWAALTSTE"
     gene            complement(2314..>3038)
                     /gene="rnhA"
                     /locus_tag="PLT69_08350"
     CDS             complement(2314..>3038)
                     /gene="rnhA"
                     /locus_tag="PLT69_08350"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOJ14485.1"
                     /translation="TVTKKKKRYYAVLRGRTPGIYTQWYGPTGALVQVEGYKDALYKG
                     FDTLEEARRFLSSPGGRSSSSRPKHKAGRVLVDAHAEKGGVVVFTDGGCIENPGPGGW
                     AAIIVRDGMCKEISGGFRLTTNNRMELYACIQALKETARDQGGVTIYTDSQYLVSAVE
                     KGWARKWRSNGWMRDKAHRALNADLWSELLELLEGRKVSFRWVRGHAGQPENERCDEL
                     ARRAARGDDLKVDEEYEKSCAR"
BASE COUNT          507 a         1053 c          917 g          561 t
ORIGIN      
        1 atcccccagg agaaaccggg cgagtccctc gagatcgtgc gcaccttctt cgaatccctg
       61 gagtgaagcg ggtacacacg cgccgcctgc ggcgccgggc acggacgggg cacaacgccg
      121 gcaccccggg cacccgctcc cgccctgtcg tcgagggttc accgctgaca cgcggagcta
      181 accgcatgtg cccgcccctc acgagcgatg ccgccggatg acggcgcaca cccgcctgca
      241 gggactcccc ctgatccacg tccacgggct gaacggcctg ccctgcctcc acgccttacg
      301 gcctgcaggc agggcggtct ccctgtcgcc tgcgcccctt cgaccacccg tggaagcggc
      361 cggccggcga cacacggcct gcatggacac ggaacgcctc cttgcgtgtc ccgtcgaatc
      421 tgcacgaagg cacctctcag agggtctctg ggaggacggg cccaccgtcg tccatgcggc
      481 atcatcgagc gccaggcatg cctggagaac cgagagggtg aacgttgtct gacgcacggg
      541 acccccaagc cactaccggc ccggatcctg tcgggggagc cagggggtcc gcctcccggc
      601 acgtgcctct cttcacaaac ttccccctgc atgccactcc cccatgaaaa cgggtgagcc
      661 gggccacact cctgaagacg gttgccggcc tcgtgcacga tgacgatcca cctgggcacg
      721 agtcgcagag gttaggccgc ggcccgagag aggaaggagg cttcgttctt tcgggccggg
      781 gcgaacgccc ctcggccgac cagggcccgg cgttcatgca ggttgcgagg tgaatctcac
      841 ggggtgagcc ggaagcccga cgtacggacg gggtctatgt cctgcgcctc ttcctcacgg
      901 ccggggtgtc ggggaagatg ctgagacctt cccggttctc cgagcagatc ctcttccact
      961 ccctgacgtc cgagaccttg tacacaacga tggcgagctc catggtgttc ttgtccgtcc
     1021 gcaccacgtc gccccagatc accacgttct cgtcctcgag caccacttct atgggcctgc
     1081 ccacgacctg cgcggagttg gcgggcgtga ggctcgtcct ggcctccatg ggcgtagcca
     1141 cgatcttgag ggagagcccc cctatcgaga tatccctggt ctcggcgagg gccacgatgt
     1201 tctggatccc cttgaggatc ttcccggagt ccctgctcga gaaggagatc ttgagcctgg
     1261 cggggatgct tgccctcttc ctctcgtgcc ttcttctctc ggccttcttc tgcccggtaa
     1321 gagacatggt tctcttttcg tcaggaatcc gggtttcctt taggaaaagg ccccttcgat
     1381 cctggtgtcg cacgcgggtc atcaccggtc ttgagacccg ctttgccgat cacacacaag
     1441 gggtgaggct cgtacgatga acccttccag caagtcgccc catgaagcgc gacgaccgca
     1501 ttcgacaacg ttgcgaacca tggcatcccc acctcggaat gggcgcccga gagccctgcc
     1561 gcgtcgaacg cgcggcagga ggtgacgccc cactgaaccc cggggacctc tcacgggccg
     1621 acagggcacg tgaggggaag ccggtcctcc ggggtgactc gccgagcctt ggggcaccct
     1681 tcagggcctg ggcatcgagc tttccagccc ttcacctcat gccgggcatg caggagtgtt
     1741 cgggtatctc tcgggcaatg cctgcttttc tttttcatgg atgtcatgta tagcaacctc
     1801 tggccgtgca tgctccgtgc gcatgccgcg tgtcctgacg atatgcgtgt gccataggtg
     1861 gagggaggag acacgatgac accaggcgat gagaccccgg aacgcagaag acaccggagg
     1921 aagcaggtcc acatcacggt cctcctcaag gtgggcgtcc tgttgaacgg caggggggtg
     1981 gcaaaggata taagcccggg aggaatgcgc ctggtctctt cccagatctt caaggccacg
     2041 accacgatcc aggccaagga ttacgagggt tcgaccatga gggtgatggt ccccaccgag
     2101 ggactgaccg tcaacggcgt catcgcatgg gtggacctca agaaggggga aggggccttg
     2161 aaggtccagt cgacttccga cgacgcgaag tgggctgcct tgacctccac cgagtgaagt
     2221 ccgcccgccg ggttgacgac gacctccccg ccgtcttcgt cttcaagacc gccgcgaagg
     2281 gttcccactc cggcagccgg cgtcatgccg cgcctagcgg gcgcaagact tctcgtattc
     2341 ctcgtccacc ttgaggtcat ccccacgggc cgccctgcgt gcgagctcgt cgcagcgttc
     2401 gttctccggc tgcccggcgt gccccctcac ccacctgaac gacaccttcc tgccttccag
     2461 gagctccagg agctctgacc agaggtcagc gttgagggcc ctgtgcgcct tgtccctcat
     2521 ccacccgttc gaacgccact tcctggccca gcccttctcc acggcggaga cgaggtactg
     2581 ggaatcggtg tagatcgtca ccccgccctg gtctctcgcc gtctccttca gcgcctggat
     2641 gcaggcgtag agctccatgc ggttgttcgt cgtgagccgg aagccacccg agatctcctt
     2701 gcacataccg tccctgacga tgatagcggc ccacccgccg ggcccagggt tttcgatgca
     2761 cccgccgtcg gtgaagacga ccacgccccc cttttctgca tgcgcatcga caaggacccg
     2821 gccagccttg tgctttggcc tgctcgacga cgatcgaccc cccggcgacg agaggaatct
     2881 cctcgcctcc tcgagggtgt cgaacccctt gtagagggcg tctttgtacc cttccacctg
     2941 cacgagcgcg ccagtgggcc cgtaccactg ggtgtatatc ccgggggtcc tgcccctcag
     3001 cacggcatag tatcgcttct tcttctttgt caccgtgg
//
LOCUS       DAOSCR010000327         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20829_length_3034_cov_2.982544, whole genome shotgun sequence.
ACCESSION   DAOSCR010000327 DAOSCR010000000
VERSION     DAOSCR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20829_length_3034_cov_2.982544"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(554..1999)
                     /locus_tag="PLT69_08355"
     CDS             complement(554..1999)
                     /locus_tag="PLT69_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ14486.1"
                     /translation="MAPLKGASGAETPTGPGMEGDTRGSAGEKGAVFVASVTSFMTPF
                     MGSSVNIALPAIGKTYAMDAVLMGWVTTSYLIATAVFLLPLGRASDLVGRKRIFVSGI
                     LVFTGVSLCIPLSWSATALIALRGVQGMGGAMIFGTAMAILASAVAPRRRGRAIGIAT
                     GATYAGLTAGPFLGGLLTEGLGWQSIFIVCALAGAAVFVVAARCVRPDTGAPSGGSFD
                     LAGASVYAGALVALMLGLARVRTASGVTLLVAGAAAMGVFLLWEGRQACPLVNTALFR
                     SNRAFTWSNAAAFIHYSATFSTSFLMSLYLQSVRGFGPSGAGVVLVTQPAVMTVLSPL
                     AGRLSDRIEPRILASLGMVITSVGLALLASLGQGTETPWIVSCLGLVGLGFSLFSSPN
                     TNAVMSSVSPGDYGMASGVLATMRITGQSFSMAVVLLVISLFLGDRGTAHVDQEAFMT
                     AMRTGFVFFSLLGIPGVLASLARGEVRKRPG"
     gene            complement(1987..>3034)
                     /locus_tag="PLT69_08360"
     CDS             complement(1987..>3034)
                     /locus_tag="PLT69_08360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ14487.1"
                     /translation="ELKGQIQQHAEGLDQLVGSHPKMQRVYEQILTAAETDLTVLILG
                     ETGTGKELVARSIHSRSERRSRPFVVINCAAIPENLLESEFFGHEKGAFSGAVSQRIG
                     KFEKAHTGTIFLDEIGDIPLDLQAKILRVLEDHKITRLGSNREIELDFRVICATNRPI
                     QDMIKKELFREDLYYRINVLPIIIPPLRERREDIPLLAGHFMAKYSKKLNSPVKGISP
                     RAMEQLERYRWPGNVREMENVIQHAMVSTRSECITDFPELRKVLEKETESATVQAQVE
                     DGSILVEAPEGNDYHSIKKALLEQFERTYFAHLLAKHGGVIAEAARESGLNYKTFYLK
                     AEKYHLTKKRGWHP"
BASE COUNT          535 a         1000 c          982 g          517 t
ORIGIN      
        1 cccgcgaaga acccctgggg gacgctgtac tgggagaacg cgagggcgct ctacacctga
       61 gaaccatgca tgaagggggg cgggctcgat gtgaccgcag gcgaagagcc cgaagcgccc
      121 tgcaccttga gacagcaccc agaccaaggg cgcaccccac cacggcgggc cggtggcacc
      181 aaccacgaaa ccgcctcatg aaacgccatc ggcccggctt gcggtccttc tccggatccc
      241 ggtcgcaagg cggcatcccg atcaacactc cacggacgcc acgcaatccc tcaccacccc
      301 aatgagcgcg ctcgaggggc atctcacacg tcatccccgt gggaaccttc tgtcgccggc
      361 tgatgcggcc cacagatctc ctcgcatgaa catgccgtga ctctgccttg tccagccggg
      421 aacctcctcc agtactgagg ccggtctggg gctcacgacc cccaggccct cgttgacaaa
      481 cgcccgcggt acgggtgcgc acgtcccgcc ccagccatga ccgaacgggt tgacgacatg
      541 tgcggaggcg gcctcagccg ggtctctttc gcacctctcc cctcgcaaga gacgcgagga
      601 cgcccggtat gccgagcagc gagaagaaca cgaaccccgt gcgcatcgcg gtcatgaacg
      661 cctcttggtc cacgtgggcc gttccacgat ctcccaggaa cagggagatc acgaggagca
      721 cgacggccat gctgaaggac tggcccgtga tccgcatggt ggcgagcacg ccggatgcca
      781 tcccgtagtc gcccggggaa acggagctca tgaccgcgtt ggtgttcggc gaggagaaca
      841 gtgagaaccc gagccccacg aggccgaggc aggagacgat ccacggcgtt tcggtccctt
      901 gccccaagga tgcgagcagg gcgaggccga cggacgttat gaccatgcca agcgatgcca
      961 ggatgcgggg ctcgatcctg tccgagagac gccccgcgag cggcgagagc acggtcatga
     1021 ccgcgggctg ggtgacgagc accacgccgg cgcctgacgg gccgaacccc ctgaccgact
     1081 ggaggtagag gctcatcaag aacgaggtgg aaaaggtggc gctgtagtgt atgaacgcgg
     1141 ctgcgttcga ccaggtgaag gcccggttcg acctgaacag tgccgtgttc acgaggggac
     1201 atgcctgcct gccctcccag agcaggaaga cccccatggc tgcggcgccc gcgacgagga
     1261 gggtgacgcc ggaagcggtc ctcacgcggg caaggccgag catgagggcg acgagggccc
     1321 ccgcatagac cgatgcacct gcgaggtcga acgacccgcc tgagggagcg cctgtgtccg
     1381 gccttacgca cctcgcggcg accacgaaga ccgcggcgcc cgccagggcg cagacgatga
     1441 agatgctctg ccacccgagc ccctcggtga ggagtccccc caggaaaggc ccggccgtga
     1501 gccccgcgta cgtggccccc gttgcgatcc cgatcgccct gcccctgcgc cggggggcga
     1561 cggccgaggc caggatggcc atggcggtgc cgaagatcat ggcgccgccc attccctgga
     1621 cccccctcag ggcgatgagg gccgtcgcgc tccacgaaag gggtatgcag aggctgaccc
     1681 cggtgaacac gaggatcccg gaaacgaaga tgcgcttcct ccccacgagg tccgaggccc
     1741 tgcccagagg caggaggaac accgccgtgg ctatgagata cgaggtcgtg acccacccca
     1801 tgagcaccgc gtccatggcg taggtcttcc ctatcgcggg caacgcgatg ttcaccgacg
     1861 accccatgaa cggggtcatg aacgaggtga cggaggcgac gaagacggcg cctttctccc
     1921 cggcactccc gcgggtgtcc ccttccatgc cgggaccggt gggtgtctct gctcccgatg
     1981 cgcctttcag gggtgccatc ctcgcttctt cgtgagatga tacttttccg ccttgaggta
     2041 gaaggtcttg tagttgaggc ccgattccct tgcggcctcg gctatgaccc caccgtgctt
     2101 ggcgaggaga tgggcgaagt aggtgcgctc gaactgctcg aggagcgcct tcttgatgga
     2161 gtggtagtcg ttcccctcgg gggcctcgac gaggatggaa ccgtcttcga cctgggcctg
     2221 caccgtcgcg ctttccgttt ccttctccag caccttgcgc agctcgggga agtcggttat
     2281 gcactccgaa cgggtggata ccatggcgtg ctggatcacg ttctccatct cgcgcacgtt
     2341 tcccggccac cggtaacgct cgagctgttc catcgccctg ggcgagatcc ccttgacggg
     2401 cgagttgagt ttcttggagt acttggccat gaagtgcccg gccaacagcg gtatgtcctc
     2461 cctgcgttcc ctcaaggggg ggatgatgat gggcaggacg ttgatgcggt agtagaggtc
     2521 ctccctgaac agttcttttt tgatcatgtc ctggatcggg cggttcgtcg cgcagatgac
     2581 cctgaagtcg agctctatct cgcggttgct cccgagcctc gtgattttgt ggtcctcgag
     2641 cacgcggagt atcttcgcct ggaggtcgag gggtatgtcg ccgatctcgt cgaggaatat
     2701 cgtcccggtg tgggccttct cgaacttgcc gatgcgctgg ctcacggcgc cgctgaaggc
     2761 gcccttctcg tggccgaaga actcggattc aagcaggttt tccgggatgg cggcgcagtt
     2821 gatcaccacg aaaggcctcg acctgcgctc gctcctcgag tgtatcgacc tggccaccag
     2881 ctccttgccc gtgccggtct cgccgaggat gagcaccgtg aggtccgtct ccgcggccgt
     2941 gaggatctgc tcgtagaccc tctgcatctt cgggtgcgac cccacgagct ggtccaggcc
     3001 ttctgcatgc tgctggatct ggcccttgag ctct
//
LOCUS       DAOSCR010000328         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20856_length_3030_cov_3.413782, whole genome shotgun sequence.
ACCESSION   DAOSCR010000328 DAOSCR010000000
VERSION     DAOSCR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20856_length_3030_cov_3.413782"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLT69_08365"
     CDS             <1..535
                     /locus_tag="PLT69_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOJ14488.1"
                     /translation="AYYLIAPAEASSNLARYDGVRYGLRLCDERDLMEMYLATRSAGF
                     GDEVKRRIMLGTYALSAGYYEAYYHKASQVRTLIAKDLREAFSRCDLLAGPTVPVTAF
                     GLGEMVDDPLSLYLLDIYTIPANLAGIPALSMPCGFDGRGLPIGLQLMAPGHREDVLL
                     GAAMTFQDHTRYHEVRP"
     gene            532..1965
                     /gene="gatB"
                     /locus_tag="PLT69_08370"
     CDS             532..1965
                     /gene="gatB"
                     /locus_tag="PLT69_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOJ14489.1"
                     /translation="MRGIEAVIGLEVHAQLKTKTKMFCGCPNSFGQELNTFTCPVCLG
                     LPGALPVLNAKAVELAVRLGLALNCRVHERSLFARKNYFYPDLPKGYQITQYDSPLCT
                     GGYLDVEAGGETRRIGIRRIHLEEDAGRSLHDEACKRTRIDFNRSGVPLVEIVTEPDI
                     SSPEEASAFLKELRSILLHLRVSEGTMEEGTFRCDANVSVRMPGDSTLGVRTELKNMN
                     SFKNVRRALAYEISRQASLLASSRPVVQETLLWSASARRTVPMRSKEESHDYRYFPEP
                     DLPPLLVDRMLIDRVRASMPELPSERRRRFMRQYGLSDYDASQVIATPELADYFEECC
                     SLVDAPTDIAHWITTELVRVMGESGDTPLTTRVRPSMLTALVSLVKAGRLSGLMAKEV
                     LGRMSKTGEDPAAAMEDLGVEQMGDEDEIRAAVRGVIDAHPDEARRYREGKTQLAGFF
                     VGEVMKVTSGRADPKLAIRIVREILDS"
     gene            1962..>3030
                     /locus_tag="PLT69_08375"
     CDS             1962..>3030
                     /locus_tag="PLT69_08375"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOJ14490.1"
                     /translation="MRKLHTAETGQVRAGEVTDVYFVRTRDVLRAKGASKRVCMEIIL
                     KSWPDPRFRWGIFAGLEEACTLLEGRPVTVHAMPEGSVFFTGEPVMTIEGDYLAFGEI
                     ETAILGCLCQASGIATQASRFRVASRGKGLISFGARRMHPSIAPMVERSAFIGGCDGV
                     ATLAAARLIGEVPVGTMPHALVLLMGDTVSAALAFDEVMGPDVPRVVLIDTFQDEKFE
                     AVRVAEALGGRLYGVRLDTPSSRRGDFRALIEEVRWELDLRGYGHVKIYVSGGLGIED
                     VLALSDVVDAFGVGTRISSAATLDFSMDVVAVEGRPLAKRGKRSGYKEVLRCTSCMRD
                     FVVQRGMTRRCACSGPTVPLLE"
BASE COUNT          543 a          930 c         1062 g          495 t
ORIGIN      
        1 cgcctactac ctcatcgcgc cggccgaggc cagctcgaac ctggcgcggt acgacggggt
       61 caggtacggg cttcgcctct gtgacgaaag ggatctcatg gagatgtacc tcgcaacgag
      121 gagcgccggg ttcggcgacg aggtgaagag gcgcatcatg ctcggcacct acgccctgtc
      181 cgccgggtac tacgaggcct actaccacaa ggcctcccag gtgcgcaccc tcatcgccaa
      241 ggacctgagg gaggccttct cccgatgcga cctccttgca ggcccgacgg tcccggtgac
      301 ggccttcggt ctcggcgaga tggtggacga ccccttgagc ctctatcttc tcgacatcta
      361 cacgataccc gcgaaccttg ccgggatacc ggcattgagc atgccctgcg gattcgacgg
      421 caggggcctg cccataggcc tccagctcat ggcgcccgga cacagggagg atgtcctgct
      481 cggggcagcc atgacgttcc aggatcacac gaggtaccac gaggtgaggc cgtgaggggg
      541 atcgaggcgg tcatcgggct cgaggtgcat gcccagctca agacgaagac gaagatgttc
      601 tgcggctgtc ccaactcgtt cggccaggag ctgaatacgt tcacctgccc ggtctgcctg
      661 gggttgccgg gcgccctgcc ggtcttgaac gcgaaggcgg tggagctggc cgtcaggctc
      721 gggctcgccc tcaactgcag ggtgcacgag aggtccctgt tcgcgaggaa gaactacttc
      781 tacccggacc tgccgaaggg ataccagatc acccagtacg acagccccct gtgcacgggc
      841 ggctacctgg acgtggaggc tggtggggaa acccggcgca taggcatcag gcgcatccac
      901 ctggaggagg atgcgggaag gagcctgcac gacgaggcct gcaagaggac gaggatcgac
      961 ttcaaccgct cgggggtccc cctcgtggag atcgtcacgg aacccgacat ctcgtcgccg
     1021 gaagaggcct cggccttcct gaaggagctc aggtcgatcc tcctccacct gcgcgtctcg
     1081 gaggggacca tggaggaagg aaccttccgc tgcgacgcga acgtgtccgt ccgcatgccg
     1141 ggagactcga cgctcggggt gaggacggag ctcaagaaca tgaactcgtt caagaacgtg
     1201 aggagggctc tcgcctacga gatctcccgc caggcgtccc tcctggcgtc ttcgagaccc
     1261 gtggtccagg agaccctcct gtggagtgcg tcagcccgcc gcaccgtgcc catgcgttcc
     1321 aaggaggaga gtcacgacta ccggtacttt cccgagcccg acctcccacc ccttctcgtc
     1381 gacaggatgc tgatcgatcg ggtcagggcc tcgatgccgg agctcccgtc agagaggcgg
     1441 aggcgtttca tgcggcagta cggcctgtcg gactatgacg cctcccaggt gatcgcgacc
     1501 cccgagctcg cggattactt cgaggagtgc tgctcgctcg tggatgcacc cacggacatc
     1561 gcccattgga tcacgacgga actcgtgagg gtgatgggcg agagcggcga cacccccttg
     1621 accacgaggg tgaggccctc gatgctcacg gccctcgtgt cgctcgtcaa ggcgggcagg
     1681 ctctcgggcc tcatggccaa ggaggtcctg ggccgcatgt cgaagacggg cgaagacccg
     1741 gcggctgcta tggaagacct cggcgtcgag cagatgggcg acgaggacga gatcagggcc
     1801 gcggtgaggg gcgtgatcga tgcgcacccc gacgaggcga ggaggtacag ggaggggaag
     1861 acgcagctcg cgggcttctt cgtgggcgag gtcatgaagg tcacctcggg cagggcggac
     1921 ccgaagctcg caatcaggat cgtcagggag atactcgact catgagaaaa cttcacacag
     1981 cggaaacagg gcaggttcgt gccggagagg tcacggacgt gtacttcgtc cgcacgaggg
     2041 atgtgttgag agcgaagggg gcatcgaagc gggtgtgcat ggagatcatc ctcaagtcgt
     2101 ggccggatcc gcgcttcagg tgggggatct tcgccggcct ggaagaggcc tgcaccctgc
     2161 tcgaggggag gccggtcacg gtccacgcca tgcccgaggg gagcgtgttc ttcacgggag
     2221 agccggtcat gaccatcgag ggtgactacc tggcgttcgg cgagatcgag acggccatcc
     2281 tggggtgcct ctgccaggcc tcgggcatag ccacgcaggc gtcccggttc agggtcgcga
     2341 gccgaggaaa ggggctcatc agcttcgggg ctcgccgcat gcatccctcg atagcaccca
     2401 tggtggaacg atcggccttc ataggcggct gtgacggggt cgccaccctg gccgccgcca
     2461 ggctcatcgg cgaggtgccg gtggggacca tgccgcacgc gctcgtcctc ctgatggggg
     2521 acacggtaag cgcggccctc gccttcgacg aggtgatggg gcctgacgtg cccagggtgg
     2581 tgctcatcga taccttccaa gacgagaaat tcgaggccgt ccgcgttgcg gaggccctgg
     2641 gcgggcgact gtacggcgtg cggctggaca cgccctcgtc acgcaggggc gacttcaggg
     2701 cattgataga ggaggtcagg tgggagctcg acctcagggg gtacggccac gtgaagatct
     2761 acgtgtcggg cgggctcggc atcgaggacg tgctggccct gtcggacgtg gtggacgcgt
     2821 tcggcgtggg caccaggata tcgagcgccg ccaccctcga cttctccatg gacgtggtcg
     2881 ccgtcgaggg ccggcctctt gcaaagaggg gcaagcgctc ggggtacaag gaggtgctca
     2941 ggtgcacctc ctgcatgcgc gacttcgtgg tgcaaagggg catgacgagg aggtgcgcgt
     3001 gttccgggcc gaccgtcccc cttctcgaga
//
LOCUS       DAOSCR010000329         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20909_length_3024_cov_3.485012, whole genome shotgun sequence.
ACCESSION   DAOSCR010000329 DAOSCR010000000
VERSION     DAOSCR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20909_length_3024_cov_3.485012"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1199
                     /locus_tag="PLT69_08380"
     CDS             210..1199
                     /locus_tag="PLT69_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ14491.1"
                     /translation="MSGLFTGLGRTVTVMWITVSATALNLVLDYGMIFGRIGLPEMGI
                     KGAALATVASGVFSMASYASLMSFGALRKRYAVLEGWRFDPELFKRLVRFGAPSGIQF
                     FLDMAGFACFVLIVGRLGTVSLAATNIAFNINTIAFMPMIGVGIAVSVLVGRYIGGGR
                     PDVAQRSTYAGFAITSAYMITVAALYVFFPSIFTAPFAHGSQAELFPQIENLTKTLLR
                     FVAVYSVFDTMNVVFSSALKGAGDTRFVMIAIVLLSAGVLVVPTFVMIAVLGMGVLAA
                     WVTATAYVSLLGLVFYMRFLAGKWRSMKVIERPCARGHIATVLPECPDSAIEP"
     gene            1551..2132
                     /locus_tag="PLT69_08385"
     CDS             1551..2132
                     /locus_tag="PLT69_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14492.1"
                     /translation="MTDGLVFMMVRMIVARLVIFMSAAFSCFLVLPCEASASPIAAEA
                     SGRLHLVTFFDRCIEETASMPAFLEEPLFVGCTQATPTRGLGQYGPGVWVIEAGMIPI
                     DIDGDEMSRVTGARLSASLFILAAGFLLMSLSYLVRSANRVGRESVLSPGKSLPATEP
                     ADFAALDGVPQESFDVLPSRGPQPAVSFTAADG"
     gene            2443..>3024
                     /gene="mazG"
                     /locus_tag="PLT69_08390"
     CDS             2443..>3024
                     /gene="mazG"
                     /locus_tag="PLT69_08390"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ14493.1"
                     /translation="MKEFDDLVHLFEVLRAPGGCPWDAAQDHESIAGCAVEEAYELLD
                     AIASGDAPHMREELGDLLLQVIFHSLIARDKGEFTLKDVIEGLSAKLVHRHPHVFGGA
                     EASTPMEVARNWEALKKEEKGATATESILDGIPRSMCALMAARKMQSAASRVGFDWED
                     ASGVIEKLREEIAELEDAISRQDRAGIKEEIGDL"
BASE COUNT          531 a          866 c          996 g          631 t
ORIGIN      
        1 tacgttgaaa ttgagaggac gaagttttct gttccctcca atgttccctc cagcggtagc
       61 ccgttgaggg gcagcctggc gccggccaac gcgcaatgag gcgtccctga tcttcagcct
      121 tcccgctctt gcccgcgagc gtggtgaaca gagcaaggcg gccgctggca ggggaatagg
      181 aatggaagcg tgctgtcgca tcggccgccc tgtcggggtt gttcacgggg ctgggccgta
      241 cggtgacggt catgtggatc acggtgtctg ccaccgcgct gaacctcgtg ctcgactacg
      301 ggatgatctt cggcaggatc ggtctgccgg agatgggcat caagggggcc gccctcgcga
      361 cggtcgcctc cggcgtgttc tccatggcct cttacgcatc cctgatgtcc ttcggtgcct
      421 tgagaaagcg ctacgctgtc ttggaaggat ggcgtttcga cccggaactg ttcaagaggc
      481 tcgtgaggtt cggcgcaccc tcggggattc agttcttcct ggacatggcc ggcttcgcct
      541 gcttcgtgct catcgtgggc aggttgggga cggtgagcct ggccgccacg aacatcgcgt
      601 tcaacatcaa cacgatcgcg ttcatgccca tgataggcgt gggtatcgcg gtgagcgtgc
      661 tcgtcggaag gtacatcgga ggcggcaggc ctgatgtcgc acagcgcagc acctacgcgg
      721 gcttcgccat aacatcggcc tacatgatca ccgtggcggc gctctacgtg ttcttccctt
      781 cgatcttcac ggcccccttc gcacacgggt cacaggcgga gctcttccct cagatcgaga
      841 atctcaccaa gaccctcctc aggttcgtgg ccgtctacag cgtcttcgac accatgaatg
      901 tcgtcttctc ctctgcgctc aagggtgcgg gcgacaccag gttcgtcatg atcgccatcg
      961 tgctcctctc cgcgggtgtc ttggtcgtcc ccacgttcgt tatgatcgcg gtgctgggta
     1021 tgggtgtctt ggcggcgtgg gtgacggcca ccgcctacgt gagcctcctc ggcctggtct
     1081 tctacatgag gtttttggcg gggaagtgga gatccatgaa ggtgatcgag cgaccctgcg
     1141 cccgtgggca catcgccacg gtgctgcccg aatgccccga ttccgccatc gaaccctgaa
     1201 gagaaagagg aaaagatttg ccaaagaagt aaaaaattac ccacacgcat ggctgcccat
     1261 gggagcggcc gtggttgatg gggggtctca agaacgtgta gtgaaacgct cgtgatcctg
     1321 tttcattccc cattccccag tgccctcttg cgggcctgat ttttgcaatt atattacacg
     1381 atcggagacc tctgccttgt cgagttgacc gttcagattg tttgccggtt gattcgttga
     1441 agatggggaa gtcgggttct ctccgtattg gtgcgtgagg agcatgcaac aaggtcggtc
     1501 acggccgcta tccctcaagg gtggtggtca caggccatgg tgatgggtac atgacggacg
     1561 gattggtgtt catgatggtc aggatgatcg tggcgagact cgtgatcttc atgtctgcgg
     1621 ctttctcgtg cttcctggtg cttccttgtg aagcctcagc atccccgatt gcggccgagg
     1681 cctcggggag gcttcacctg gtgaccttct tcgaccgctg catagaggag accgcctcga
     1741 tgcccgcctt tctcgaagag cctctcttcg tgggatgcac gcaggcgacc cccacccggg
     1801 gtctcgggca gtacgggccg ggcgtgtggg tgatcgaggc cgggatgatc cccatcgaca
     1861 tcgacggcga cgagatgtcc agggtgacgg gtgcccgcct gtccgcgagc ctcttcatcc
     1921 tggctgccgg attcttgctc atgagcctgt cttacctggt gcgctctgcg aaccgagtgg
     1981 gccgggagag cgttctttcg cctgggaagt ccctgccggc caccgaaccg gcagacttcg
     2041 cagccctcga cggcgttccg caggaaagct ttgacgtact cccctccagg ggaccccagc
     2101 cggcagtctc cttcaccgca gcggacggct gagggggcac tgttcaagga aatcgggtcg
     2161 ggatcctcgt tccgggcagc catgcacgcc gccgatgtgc ggtggtgatg cgggcttcgt
     2221 ctctcgcaac cagcgttcag gcatgccgcc tgtggtgatc gaggatctcc tgcgtgcggg
     2281 aaggaacggc atgccagggg cgaccctgtc tgggcgtgga tctgcccctt tgccgtcaga
     2341 gatcgagggc gtgaacggga aggagcccgt cgagctcaaa tgggttgagg aacgaggcgg
     2401 tgttgtgtca tgattcccac tcggcgggag gagacgctca acatgaagga attcgacgat
     2461 ctggtacatc tcttcgaggt gctcagggcg ccagggggct gcccgtggga tgcggcccag
     2521 gaccatgagt ccatagccgg gtgcgcggtg gaggaggcat acgagctcct cgatgccata
     2581 gcgtcggggg atgcgcctca catgcgcgag gagctcggag atcttctgct ccaggtgatc
     2641 ttccacagcc tgatcgcgag ggacaagggt gagtttaccc tcaaggacgt catcgagggc
     2701 ctttcggcga agctcgtgca ccggcaccct catgtcttcg gcggggcaga ggcgtccacc
     2761 cccatggagg tggcaagaaa ctgggaggca ttgaagaagg aagagaaggg ggccacggca
     2821 acagaatcga tcctcgacgg catcccgaga tccatgtgcg cactcatggc ggcccggaag
     2881 atgcagtccg cggcctccag ggtggggttc gactgggagg acgcctcggg ggtgatcgag
     2941 aagttgagag aagagatcgc agagctcgaa gatgcgatct cgaggcagga ccgtgcaggg
     3001 atcaaggagg agattggaga cctg
//
LOCUS       DAOSCR010000330         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20954_length_3019_cov_2.977058, whole genome shotgun sequence.
ACCESSION   DAOSCR010000330 DAOSCR010000000
VERSION     DAOSCR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20954_length_3019_cov_2.977058"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..336)
                     /gene="rpmA"
                     /locus_tag="PLT69_08395"
     CDS             complement(76..336)
                     /gene="rpmA"
                     /locus_tag="PLT69_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ14494.1"
                     /translation="MAHKKSGGTSRNGRDSAGKRFGVKRYAGQFVKAGNILVRQCGTK
                     IHPGENVGCGRDYTLFALKDGVVRFETKETTNRKVKIVSVTA"
     gene            complement(343..663)
                     /gene="rplU"
                     /locus_tag="PLT69_08400"
     CDS             complement(343..663)
                     /gene="rplU"
                     /locus_tag="PLT69_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ14495.1"
                     /translation="MYAVIRTGGKQYKVEEGDILRVEKLDAEKGDTVTFDDVLLIGGD
                     GYVLGRPTVEGARVSASVVRQMRDRKIIVFKMKRRKRYRRTQGHRQYLTEVRITKISK
                     NQEA"
     gene            complement(728..1282)
                     /locus_tag="PLT69_08405"
     CDS             complement(728..1282)
                     /locus_tag="PLT69_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14496.1"
                     /translation="MLELALHVLDIVENSARAGATLVKISVVEDLEGDVLSIRIEDNG
                     AGMDEETRARALDPFYTTKKVRRIGLGLPMLKDAAERAGGTFSLDSEPSKGTVVHATF
                     RHTHIDRQPLGDMAGVIAAIMLGNPHMDIVYTHSRGGRSYELDTREIREGLGGVALNE
                     PEVVRLVRENVRESLLEIGAGNTP"
     gene            complement(1287..2024)
                     /locus_tag="PLT69_08410"
     CDS             complement(1287..2024)
                     /locus_tag="PLT69_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ14497.1"
                     /translation="MGRVYRCNLHIHTCLSPCGSLDMHPASIVSACRDAGLDMIAVTD
                     HNASENVRYVQEAARKTGLVVIPGMELCTREEVHILALFERLDQLEALQDIVYEHLSG
                     VNDERVFGVQAVVNEHGEVEGFNEHLLIAATELSLEDAVRHIHGLDGLAVPSHIDRTA
                     FGVIGQLGFVPNDVPFDALEVSCRMGVKQARREYPELAGYTMITSSDAHELVDIGRAC
                     TRFFIDSPTFPEIRLAFKKSLGRYCLE"
     gene            complement(2058..2402)
                     /locus_tag="PLT69_08415"
     CDS             complement(2058..2402)
                     /locus_tag="PLT69_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOJ14498.1"
                     /translation="MNLDTLAKRLDLSCKCCWEKLNREVTGGYTGDLLSDVMANSKAG
                     DIWVTRQVHKNVVAVAALKEHAAVVVVQGAEPDVDALEKATAEGIPIFTTNKSAFEIT
                     GLIHGLLREDKA"
     gene            complement(2471..2944)
                     /locus_tag="PLT69_08420"
     CDS             complement(2471..2944)
                     /locus_tag="PLT69_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14499.1"
                     /translation="MQLHEDERYLQGRYEVQGRDFVDAGLVSSLIKKALKDKGIDEDV
                     IRRVAIVAFEAEVNIISYAESGTISLYLLPDSVVFEAKDVGPGIADIELALQEGYSTA
                     DDTIREMGFGAGMGLSNIKKSSDVFEISSEVGKGTCLRSVIRLDGGETPGEGDGR"
BASE COUNT          486 a         1014 c          860 g          659 t
ORIGIN      
        1 cctgaaagaa cagcagccgt tcccgccggc accagccctg acgtagatcc tggactcgtc
       61 gacgaagtcc atgcctcagg cggtgacgga gacgatcttt accttcctgt tcgttgtctc
      121 tttggtctcg aacctgacca cgccgtcttt cagggcgaac agggtgtagt ccctgccgca
      181 ccccacgttc tcaccggggt ggatcttggt tccgcactgc cgaacaagga tgttgcccgc
      241 cttcacgaac tggcctgcgt agcgctttac cccgaaccgc tttccggcgc tgtccctgcc
      301 gttgcgcgag gtcccgcccg atttcttgtg tgccatggcc tgttacgcct cctggttctt
      361 cgaaatcttg gtgatgcgca cctcggtgag gtactgccgg tgaccctggg tcctcctgta
      421 gcgcttcctc ctcttcatct tgaagacgat gatcttcctg tcgcgcatct gacgcaccac
      481 cgaagcgctg accctggcgc cctcgacggt gggcctgccg aggacgtacc cgtcaccccc
      541 tatgagcagg acatcgtcga aggtgaccgt gtcccccttt tctgcgtcga gcttctccac
      601 gcggaggatg tctccctcct cgaccttgta ctgctttcca ccagtcctta tgaccgcata
      661 catcgtgcac ctcgaaagcg tggtttggct gagaatggtg agctattaat gaaatctcct
      721 atgcttgtca aggagtattc cctgcgccga tctccaggag ggactccctc acgttctcgc
      781 gaaccagcct gacaacctcg ggctcgttca acgccacgcc gcccagcccc tcccgtattt
      841 cccgggtgtc gagctcgtag ctccgccctc ccctgctatg ggtgtagacg atgtccatgt
      901 gagggttccc gagcatgatc gcagcgatga cgcctgccat gtcgccgagc ggctgccggt
      961 ctatgtgggt gtgcctgaac gtggcgtgca ccacggtgcc cttcgacggc tcggagtcca
     1021 ggctgaacgt ccccccggca cgctcggccg cgtccttgag catggggagg cccaggccta
     1081 tgcgcctgac cttcttggtc gtatagaagg ggtcgagcgc gcgcgccctc gtctcctcgt
     1141 ccatgccggc gccgttgtcc tcgatccgta tcgagaggac gtccccttcg agatcctcca
     1201 ccacgctgat cttcacgagg gtcgcgccgg ccctggccga gttctcgacg atatcgagca
     1261 cgtggagagc caattccaac atggtttcac tccaggcaat accttccgag gctcttcttg
     1321 aaggccaggc ggatctccgg aaacgtgggg gaatcgatga agaagcgggt gcatgccctg
     1381 ccgatgtcca cgagttcgtg ggcgtccgaa gaggtgatca tcgtgtagcc ggcgagttca
     1441 gggtattccc tgcgcgcctg cttcaccccc atcctgcagg acacctcgag ggcgtcgaac
     1501 ggcacgtcgt tcgggacgaa accgagctgt cctatcaccc cgaaggccgt cctgtcgatg
     1561 tgagagggga ccgcgagacc gtcaagcccg tgtatgtgac gcacggcgtc ttccagggac
     1621 aactcggtgg ccgcgatgag gaggtgctcg ttgaaccctt ccacctcccc gtgctcgttg
     1681 accacagcct ggacgccgaa gacccgctcg tcgttcaccc cgctcaagtg ttcgtacacg
     1741 atgtcctgca gggcctcgag ctggtccagg cgctcgaaca gggcgaggat gtgcacctcc
     1801 tcccgggtgc agagctccat gcccgggatc accacgagac cggtcttgcg cgccgcctcc
     1861 tgcacgtacc tgacgttctc cgaggcgttg tgatcggtca cggctatcat gtcgagccct
     1921 gcatccctgc atgcgctcac gatcgatgcg ggatgcatgt caaggctgcc gcacggcgag
     1981 aggcacgtgt ggatatggag gttgcagcgg tacacccttc ccatggtctc tctcggggtt
     2041 atgggcccaa gccctcttca ggccttgtcc tcgcggagaa ggccgtggat gagcccggtg
     2101 atctcgaagg cgctcttgtt cgtcgtgaag atcgggattc cttccgccgt ggccttctcc
     2161 aaggcgtcga cgtcgggctc ggcaccctgg acgaccacga cggccgcgtg ctccttgagg
     2221 gccgccacgg cgaccacgtt cttgtggacc tgcctggtca cccatatgtc cccggccttg
     2281 ctgttggcca tgacatcgct caggagatcc ccggtgtatc cgcccgtgac ctccctgttc
     2341 agcttctccc agcaacactt gcaggaaagg tcgagccgtt tcgccaaagt gtccaggttc
     2401 atgtcacctc ccggtgagcc gcgtggtatg gtgccggccg aagatgggcg atcgcccccc
     2461 gccctgacgg tcatcgacca tccccctctc caggcgtttc gcctccatcg agcctgatca
     2521 cgctcctcaa gcacgtgcct ttgccgactt ccgaagagat ctcgaagaca tctgaggatt
     2581 tcttgatgtt cgacaggccc atccccgctc cgaatcccat ttccctgatc gtgtcgtccg
     2641 ccgtcgagta cccctcctgg agggcgagct cgatgtcagc gatgccggga ccgacgtctt
     2701 tcgcctcgaa gaccaccgag tccggcagga ggtagaggct gatggtcccg gactcggcgt
     2761 aagagatgat gttgacttcg gcctcgaacg ccacgatggc cactctcctg atcacgtcct
     2821 cgtcgatgcc cttgtccttg agggccttct tgatgaggct cgagacgagg cctgcatcga
     2881 cgaagtccct tccctggact tcgtagcgac cctggagata tctctcgtct tcatgcagtt
     2941 gcatcggaga ctacctctga ctttcctctt ctttctctcg cgctgggttc gacacccgcc
     3001 atccagagcc tgccgctcg
//
LOCUS       DAOSCR010000331         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_20982_length_3015_cov_3.162838, whole genome shotgun sequence.
ACCESSION   DAOSCR010000331 DAOSCR010000000
VERSION     DAOSCR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20982_length_3015_cov_3.162838"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLT69_08425"
     CDS             <1..473
                     /locus_tag="PLT69_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HOJ14500.1"
                     /translation="MESPAPLARISCLVKPTQTRRPGLQGALQWRLISHLSLNHLSLV
                     SGGADPLKEILSLYDFEDSPSTRQQISGIVGLDTRHVTRRVKGSFCRGLLVRVTFDED
                     KFVGSSMFLLASVLERFLAHYVSINSFVQLEALSLQRRGAVRLWPPRNGNRILA"
     gene            470..1483
                     /gene="tssG"
                     /locus_tag="PLT69_08430"
     CDS             470..1483
                     /gene="tssG"
                     /locus_tag="PLT69_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HOJ14501.1"
                     /translation="MSDMTIGERLFARPGHFSFFEAVHLLELFSGRPGGLGTEASPAR
                     EPVRFKVRPSLVFPASEIASLEPGGDGSPPEMEVTFMGLVGPSGVLPHWYTELALERL
                     RQKDGAMAAFLDIFHHRLVTLFYLAWKRTRLCENHDPGKPDRLTGCLMSLLGLATGGD
                     PGLEGPVIETIIFHAGIMGTRVANAWSVESAVGYLTGVPVEVEQFVERTVEVDRDDTT
                     RLGQACCTLGVDAQAGGYARDASGFFRVHLGPMGYDEFTRFLPGGAMLPRVFSLVRFK
                     VGPEYEFDLKVILDRREVPPCVLGSGSRLGMTSWLKAPGRPHAEDVFVIVGERQALPA
                     SSV"
     gene            1632..>3015
                     /locus_tag="PLT69_08435"
     CDS             1632..>3015
                     /locus_tag="PLT69_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14502.1"
                     /translation="MSNLKTLIGKLNPTCRSALESAAGLCMSQTHYEVDIEHFLLKLL
                     DLKDTDLHRILSRFEINGDRLRADLTRAMEEFKTGNARTPVFSPRIPKMIEDAWMVAS
                     IDFAQDRIRSGHIVLALFRNEQTGRMLASESASLAKIKAQALEESLPDIVRGSSEDAG
                     AMPAAQGPAAEPAVTGGTRALDLYTVNLTELARSGRIDPVLGRDFEIRQMVDILIRRR
                     QNNPILTGEAGVGKTAVVEGFAMRVAQGDVPPALRDVDIRSLDLGLLQAGAGVKGEFE
                     NRLKSVINEVKASPKPIIMFIDEAHTLIGAGGAAGMGDAANLLKPALARGELRTIAAT
                     TWAEYKKYFEKDAALARRFQVIKVEEPDEEKTCVMMRGIAPFLENHHKVTITDEALEA
                     AVKLSHRYISGRQLPDKAVSVLDTACARVAIGLTTSPPALEEANRRITHIKGELRALE
                     KETLTGADHTE"
BASE COUNT          541 a          987 c         1010 g          477 t
ORIGIN      
        1 ccatggagtc gccggcgccc ctcgcaagga tctcctgcct ggtgaaaccg acccagacga
       61 ggaggccagg gcttcaaggg gccctccaat ggaggctcat ctcccacctg agcctgaacc
      121 acctctctct cgtctcgggc ggggcggacc cgttgaagga gatcctcagc ctgtacgact
      181 tcgaggactc cccgtcgaca cgccagcaga taagcggcat cgtcggcctc gacacccgcc
      241 acgtgaccag gcgcgtgaag gggtcgttct gccgcggcct gctggtccgc gtgaccttcg
      301 acgaggacaa gttcgtgggc tcgagcatgt tcctcctcgc gagcgtcctc gaacggttcc
      361 tcgcccacta cgtctccatc aactcgttcg ttcagctcga ggccctctcc ctgcagagga
      421 ggggggccgt gaggctctgg ccgccaagga acggcaacag gatcctcgca tgagcgacat
      481 gaccataggg gaaaggctct ttgcaaggcc cgggcacttc tcgttcttcg aggcggtgca
      541 cctcctggag ctcttttcgg ggagacccgg cgggctgggc acagaggcga gccctgcgag
      601 ggaaccggtg cggttcaagg taaggccctc gctggtcttc cccgcctcgg agatagcgtc
      661 gctggagccc ggcggggatg ggtcgccccc cgagatggag gtcaccttca tgggcctcgt
      721 ggggccctcg ggcgtgcttc cccactggta cacggaactc gcgctcgagc gcctcaggca
      781 gaaggacggg gccatggcgg cgttcctcga catctttcac caccggctcg tcaccctgtt
      841 ctatctcgcc tggaagagga cccgcctctg cgagaaccac gaccccggga aaccggacag
      901 gctcaccggg tgtctcatga gcctgctcgg gctcgcgacg ggcggcgacc ccggcctcga
      961 gggcccagtg atcgagacga tcatcttcca cgccggcatc atggggacga gggttgccaa
     1021 tgcgtggtcc gtggagtcgg cggtgggcta tctcaccggg gtgcccgtcg aggtggaaca
     1081 gttcgtcgag cgaaccgtgg aggtggaccg agacgacacc accaggctcg gccaggcgtg
     1141 ctgcaccctc ggggtggacg cgcaggccgg ggggtacgcg agggacgcct cgggcttttt
     1201 cagggtgcac ctcggcccca tggggtatga cgagttcacc cggttcttgc ccgggggcgc
     1261 catgctcccc agggtgttct cgctcgtgag gttcaaggtg ggccccgagt atgagttcga
     1321 tctcaaggtc atcctggaca ggcgcgaggt gccgccgtgc gtgctcggct ccggttcgag
     1381 gctgggcatg acgagctggc tcaaggcgcc tgggagaccc catgccgagg acgtcttcgt
     1441 catcgtcggg gagcgccagg ccctaccggc ctcatcggta tgaagggggt gcacggcacc
     1501 cgaagggtgt cttcccgcga tcggccaggc gagaggaggg gcgccgccgg tcccgcactt
     1561 gattctgtgg tgaggctgcc atgtgtggtc gcccgggcgg ttgtccttaa aagaaaggag
     1621 gtcaccagac cgtgtcgaac ttgaagaccc tgatcggaaa gctcaaccct acctgcaggt
     1681 ccgccctcga atcggcggcc ggcctgtgca tgtcccagac ccactacgag gtggacatcg
     1741 agcacttcct cctcaagctc ctggacctca aggacacgga cctccaccgg atactaagcc
     1801 gcttcgagat caacggggac aggctcaggg ccgatctcac gcgcgccatg gaggagttca
     1861 agacgggcaa cgcccgtacc cccgtcttct cgccgcggat accgaagatg atcgaagacg
     1921 cgtggatggt cgcgtccatc gatttcgcgc aagaccgcat ccgttccggg cacatcgtgc
     1981 tggccctctt caggaacgag cagaccggcc gcatgctcgc gtccgagagc gcctcgctcg
     2041 ccaagataaa ggcacaggcg ctcgaggaat cgctcccgga catcgtgagg gggtcttccg
     2101 aggacgccgg tgccatgccc gcggcgcaag gcccggcggc ggaacccgcc gtaaccggtg
     2161 ggaccagggc cctggacctc tacaccgtga acctcacgga actcgccagg tccggcagga
     2221 tagaccctgt cctcgggagg gacttcgaga tacggcagat ggtcgacatc ctcatccggc
     2281 gccggcagaa caaccccatc ctcaccggtg aggcgggtgt cgggaagacg gccgtggtcg
     2341 aggggttcgc catgagggtg gcccaggggg acgtgccccc tgcgctcagg gatgtcgata
     2401 tccgttccct ggacctcggt ctcctgcagg ccggggcggg ggtcaagggc gagttcgaga
     2461 accgactcaa gtcggtcatc aacgaggtga aggcctcccc caagcccatc atcatgttca
     2521 tagacgaggc gcacaccctc ataggcgcgg ggggcgcggc cggcatgggc gacgccgcca
     2581 acctcctgaa gcccgcgctg gccagggggg agctccggac catagcagcg acgacctggg
     2641 cggagtacaa gaagtacttc gagaaggacg ccgccctggc gcgcaggttc caggtgatca
     2701 aggtggagga gcccgacgag gagaagacct gcgtcatgat gcgcggcata gcgccgttct
     2761 tggagaacca ccacaaggtg accataactg acgaggccct ggaagccgcc gtcaagctct
     2821 cgcaccgcta catctccggc aggcagttgc ccgacaaggc ggtgagcgtg ctcgacaccg
     2881 cgtgcgcgcg cgtcgccatc ggcctgacca cgagcccccc tgccctcgag gaggcgaacc
     2941 gccggataac ccacatcaag ggagagctca gggcgctcga aaaggagacg ctcacgggcg
     3001 ccgaccacac ggaga
//
LOCUS       DAOSCR010000332         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21014_length_3012_cov_3.746026, whole genome shotgun sequence.
ACCESSION   DAOSCR010000332 DAOSCR010000000
VERSION     DAOSCR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21014_length_3012_cov_3.746026"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="PLT69_08440"
     CDS             <1..1839
                     /locus_tag="PLT69_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ14503.1"
                     /translation="CFRERRPLLIEDAHEITKDLSERSQELLMLSESSSFICVPIVFE
                     QEPLGILVVDRTFRQTPLRQNDVKLLLGIAPQIAICINNARTFERLQESEEKYRDLVE
                     SAASIILRISTEGKITFANRFACEFYGYEEDEMIGQDVVGFMIPRRDSSGRDMAEIVG
                     RFLAEPVLAAGVENENIRRNGERVWVSWSMRPVRGKGGAINEILCVGNDVTARKAAEQ
                     EKRQLEVQLVRSQKMEAIGNLAGGVAHDLNNILSGITGYPELLLMELPEGSPLRKVAL
                     TIKKSGEKAAAMVQDLLTLARRGVGKSGVVDINRVVSDYLESPEFQKLKEYHPGVSFK
                     VDLQETVGLIAGSELHLGKTLMNLVSNAAEAIPSGGEVTIRTSTRRLDQPLKGFDTVG
                     PGVYTVLTVEDTGMGITEEDQKKIFEPFFTKKVMGRSGTGLGMTVVWTTVKDHKGHID
                     IVSAPGEGTRIDLYFPVTTEKAVHSQDSAPITDFAGTESILVVDDVEDQRELVKNILM
                     RLGYTVHTVSTGEDAVEFVSRRRCDLVVLDMILGPGMDGLDTYRKIVEATGPMKAVIV
                     SGFSENERVKAARELGVGRYVHKPYTLEALARAIREELDAKPKGQA"
     gene            2219..3007
                     /locus_tag="PLT69_08445"
     CDS             2219..3007
                     /locus_tag="PLT69_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14504.1"
                     /translation="MNITYRFLVFQSLVVLPFLLGAGIRRIRGQSWATGMTKRLVNVN
                     LVAIEPVVALWSTWGLVLTAQLAVLPVAGLILVSAGFLLGMAAGKVLGLDTRRKAAFH
                     VSSSLSNHGFTMGAFFCYLVLGERGLGLSSVFLAYFMPFVFLVIFPYAHRKSSRAAGG
                     IPSAGEFFLSLKNMPLYAVIGATVLLVLGIERPRVPFPLDAFIMLSMVLYYFALGLSF
                     DPALRGAGAGSTPRSFPSSSSPCLLCARSCSHAYTSILRSRLSS"
BASE COUNT          594 a          911 c          960 g          547 t
ORIGIN      
        1 tgtttcaggg agagaaggcc cctcttgata gaagacgcgc acgagataac gaaagacctc
       61 tccgagagga gccaggagct gctcatgctc tccgagtcgt cgtccttcat ctgcgtgccg
      121 atcgtcttcg agcaggaacc cctgggcatc ttggtcgtcg accggacgtt caggcagacg
      181 cccctcaggc agaacgacgt gaagctgttg ctcggcatag ccccgcagat cgccatctgc
      241 atcaacaacg cccgcacgtt cgagaggctg caggagagcg aagaaaagta ccgtgatctc
      301 gtggagagcg cggcgagcat cattctcagg atcagcaccg aggggaagat aacgttcgcg
      361 aacaggttcg catgcgagtt ctacgggtac gaagaggacg agatgatagg ccaggacgtg
      421 gtcgggttca tgatcccccg cagggactca tcgggaaggg acatggccga gatcgtgggc
      481 aggttcctcg ccgagcccgt ccttgccgcc ggggtggaga acgagaacat caggcgcaac
      541 ggcgagcggg tgtgggtcag ctggagcatg aggccggtgc ggggaaaagg cggcgcaata
      601 aacgagatcc tgtgcgtggg aaacgacgtc accgccagga aggcggccga acaggagaag
      661 agacagctcg aggtccagct ggtgaggtcg cagaagatgg aggccatagg caacctcgca
      721 ggcggcgtcg cacacgatct caacaatatc ttgagcggta tcaccgggta tcccgaactg
      781 ctgctgatgg agctccccga ggggtctccc ctcaggaagg tggccctcac gatcaagaag
      841 tccggcgaga aggcggcggc tatggtccaa gacctgctca ccctcgcgag gaggggggtg
      901 gggaaatccg gcgtggttga catcaaccgc gtggtgagcg actacctcga gagcccggag
      961 ttccagaaac tgaaagagta ccatcccggt gtgtctttca aggtggacct gcaggagacg
     1021 gtgggactca tcgcgggctc ggagctccac ctcgggaaga ccctcatgaa cctcgtatcg
     1081 aacgctgccg aggcgatacc ctccggaggc gaggtcacca tacgcacttc aaccaggcgt
     1141 ctcgaccagc cgctcaaggg gttcgacacg gtcggccccg gcgtctacac ggtccttacc
     1201 gtggaggaca cggggatggg aatcaccgag gaagaccaga agaagatctt cgagcccttc
     1261 ttcaccaaga aggtcatggg acgaagcggt accggcctcg gcatgaccgt cgtctggacc
     1321 acggtcaagg atcacaaggg acacatcgac atcgtgagtg cgccagggga ggggacgagg
     1381 atcgatctct atttcccggt cacgaccgag aaggccgtgc acagccagga ctctgcgccg
     1441 ataacggatt tcgcgggaac agagagcatc ctcgtcgtcg acgacgtgga ggatcagaga
     1501 gagctggtca agaacatcct tatgaggctc gggtataccg tgcacacggt ctccactggg
     1561 gaggatgccg tcgagttcgt ctccaggcgc cgatgcgacc tcgtcgtcct cgacatgatc
     1621 ctgggcccgg ggatggacgg tctcgacacg tacaggaaga tcgtcgaggc aacaggcccc
     1681 atgaaggcgg tcatcgtgag cggtttctcc gagaacgagc gtgtcaaggc ggcgcgtgag
     1741 ctcggcgtgg gcaggtacgt gcacaagccc tacaccctgg aggcactcgc cagggcgata
     1801 cgcgaagagc tcgacgccaa gccaaaggga caggcctgag cacccttcga gaaacccgta
     1861 ccttgaaagg atcttctttt tcccgtctca ccactatcct cgatccccat acccggcggc
     1921 gttccgccga aactgtcgga ccctgtgccg gtgccgagcc ctcgatcccc gcacccggag
     1981 gcgtttttcg gcgagcgaac atgcccgggc cttagaaccc cttggtgcag gcatgggcag
     2041 gactcgatcg agcacggcgg cgtgccttag gcctgcccgc acgaaacgag gacaggcctc
     2101 ccggccgggt atgcccgggc caggccccat gcggcctcac accgcaccac cggaccgtac
     2161 cgcgggcaac cccgatatcg aggcgcgatc gcggccacgg tatggtataa ggccatgcat
     2221 gaacatcacc taccggttct tggtgttcca gtctctcgtc gtcctgccct ttctcctggg
     2281 tgcgggcatt cgaaggatca ggggacagtc atgggctacc gggatgacca agaggctcgt
     2341 gaacgtgaac ctcgtggcaa tagaaccggt ggtggccctg tggagcacct gggggctcgt
     2401 gctgaccgcc cagctcgccg tgctgcccgt ggctggactg atcctcgtga gcgcgggttt
     2461 tctcctcggc atggccgcgg ggaaggtcct cggcctcgac acgagacgca aggccgcctt
     2521 tcacgtgagt tcttccctct ccaaccatgg gtttaccatg ggcgccttct tctgctacct
     2581 cgtcctcggc gagcgggggc tcgggctctc gtccgtcttc ctcgcgtact tcatgccgtt
     2641 cgtctttctc gtgatctttc cctatgcaca caggaagtct tcgagggccg caggagggat
     2701 accgtccgcg ggggagttct tcctcagtct caagaacatg ccgctctatg cggtgatagg
     2761 tgcgaccgtg ctcctcgtgc tcgggatcga acgtccaagg gtgccgtttc ccctggacgc
     2821 attcatcatg ctttccatgg tgctgtacta cttcgccctc ggtttgagct tcgacccggc
     2881 tctcaggggg gcgggggccg gcagcacgcc gcgctctttt ccatcaagtt cgtcgccgtg
     2941 ccttctctgt gcgcggtcgt gctcgcatgc gtacacctcg atcctgcgat cgagactgtc
     3001 atcttgatcc ag
//
LOCUS       DAOSCR010000333         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21114_length_3002_cov_3.985409, whole genome shotgun sequence.
ACCESSION   DAOSCR010000333 DAOSCR010000000
VERSION     DAOSCR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21114_length_3002_cov_3.985409"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLT69_08450"
     CDS             <1..587
                     /locus_tag="PLT69_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HOJ14505.1"
                     /translation="SHFMEKFSEIGKGTQAFSFDNLDKDSLRFLADLYEKEVRPYLKV
                     PQLGLMRFHQERMNEAFDAWALFQTKLAAFLRLVGLPVEKSVKVFQETVERQIREGGV
                     PDDPRKLYTTWIKILEGHFMTLFKSPEYTAMLADTLASLESFLAARNRVLEDLLQSLP
                     IPSSREVDELAKELHDLKRRVRDLEKAAQGPRRA"
     gene            603..1811
                     /gene="phaC"
                     /locus_tag="PLT69_08455"
     CDS             603..1811
                     /gene="phaC"
                     /locus_tag="PLT69_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HOJ14506.1"
                     /translation="MAGHPRIPVDLILEKLAQEAEKAQQRAGEVSRVLLGRLSTDIAT
                     TPYEVVHQVDRVKLKHYLPTREANPELRTPLLVVYALINRETMLDLQPERSVVRNFLQ
                     AGIDLYMIDWGYPSRKDRFLTIDDHVNVYMDEMVEFIRRRCRTDKVNLMGICMGGTFC
                     VIYSALHPHKVKNLVTTVCPTNFDTDEGLLHIWMRQVDVDMMVDTFGNMPGDLLNLGF
                     LLLNPARLMIDKYVGFLENMNDRSFVENFIRMEKWIFDSPDVPGETFRQFVKDCYQQN
                     LLIQNRLVVGSRRVDLKKITMPLLNIYGQYDHLVPPGAANLLTKAVGSEDTTDICLDT
                     GHIGIYVSSKTQREFTPAIVTWLKTRDKAPPARKEGDGRAARKADRPKKRAWTERTRP
                     PVGRKERVNV"
     gene            1804..2856
                     /locus_tag="PLT69_08460"
     CDS             1804..2856
                     /locus_tag="PLT69_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ14507.1"
                     /translation="MSEKSDYFDTFCAVSRALGTTLNRQRVLDLIVENAIKTMDGKAA
                     CLFFADEDGASDVFVPLAQKGLSETYLHAEPQEARRAAQEIMARGYIAIHDATKDPRI
                     KTHEQKKKEGIASILVVPVVVKDMVKGVLALYTSKPRIFSQDEIRFLTALAEQGGLAI
                     ERARLVMQITRNIRIFTRVAEGLNESLDIKKILERLSVDMRQALGVKAVSVRLVNKDT
                     GTLELVASNGLSRKYLAKGPVRIEKSVTAALQGRTVVTRDATKDKDIQFKEEKKKEGI
                     VSILTVPIKVKDEVIGVMRYYSGTERDFTEGEVMLAEAVASLGGLAIHNASMYLMLKN
                     DLQCLKEDLWSHRSWF"
BASE COUNT          668 a          916 c          944 g          474 t
ORIGIN      
        1 agtcccactt catggagaag ttctcggaga tcggcaaggg gacgcaggcg ttcagcttcg
       61 acaacctcga caaggacagc ctgcgcttct tggctgatct ctacgagaag gaggtcaggc
      121 cctacctcaa ggtgccccag ctcggcctca tgcggttcca ccaggaacgc atgaacgagg
      181 ccttcgatgc gtgggcgctc ttccagacca agctcgcggc gttcttgcgc ctcgtgggcc
      241 tgccggtgga gaaatcggtc aaggtgttcc aggagacggt cgagcggcag atccgcgagg
      301 gtggggttcc ggacgacccg cgcaagctgt acaccacctg gatcaagatc ctcgaaggtc
      361 acttcatgac cctcttcaag tcacccgagt acaccgccat gctcgcagac acactggcct
      421 ccctcgaatc ctttctcgcc gcaaggaaca gggtgctcga ggatctcctg caatcgctcc
      481 ccatcccctc gagcagggag gtggacgagc tggcgaagga actccacgac ctgaagagac
      541 gcgtgagaga cctggaaaag gctgcacagg gccctcgcag ggcctaaggc ggaggacgac
      601 gtatggcggg acatccgcgg atacccgtgg atctcatcct cgagaagctc gcccaagagg
      661 ctgaaaaggc acaacagcgc gccggcgagg tctcgcgggt gctgctcgga cggctcagca
      721 ccgacatcgc caccacgcca tacgaggtcg tgcaccaggt ggaccgcgtc aagttgaagc
      781 actacctgcc gaccagggag gcgaaccctg aactgaggac gccgctcctt gtcgtctacg
      841 ccctcatcaa cagggaaacg atgctcgatc tccagcccga gcgcagcgtg gtgcgaaact
      901 tcctccaggc gggcatcgat ctctacatga tcgactgggg atacccgagc cggaaggacc
      961 ggttcctcac catcgacgac cacgtgaacg tctacatgga cgagatggtg gagttcatcc
     1021 ggagacgatg caggacggac aaggtgaacc tcatgggcat ctgcatgggc ggtaccttct
     1081 gcgtcatcta ctccgccctg catccgcaca aggtcaagaa cctcgtgacg acggtctgcc
     1141 ccacgaactt cgacacggac gaggggctct tgcacatctg gatgaggcag gtggacgtgg
     1201 acatgatggt ggacaccttc gggaacatgc ccggcgacct cctcaatctc ggctttttgc
     1261 tcctcaatcc agccaggctc atgatcgaca agtacgtggg gttcctggag aacatgaacg
     1321 ataggtcctt cgtcgagaac ttcatccgca tggagaagtg gatcttcgac agcccggacg
     1381 tgcccggcga gacgttcagg cagttcgtga aggactgcta ccaacagaac ctcctcatcc
     1441 agaacaggct cgtggtcggc tcccggcggg tggacctcaa gaagatcacc atgccactcc
     1501 tcaacatcta cgggcagtac gaccatctcg tgccgcccgg tgcggcgaac ctcctcacca
     1561 aggcggtcgg gagcgaggac acgacggaca tatgcctcga taccgggcac atcggcatct
     1621 acgtgagttc caagacccag agggaattca cgcccgcgat cgttacgtgg ctgaagacgc
     1681 gcgacaaggc tccccccgcc cgcaaggagg gggacggtcg cgcggcgcgc aaggcggaca
     1741 ggccgaagaa gagggcatgg accgaacgga cgaggccgcc ggtcggtcgg aaggagagag
     1801 tgaatgtctg aaaagagcga ttacttcgac accttctgcg cagtgagcag ggccctgggg
     1861 accaccctga acaggcagag ggtcctcgac ctcatcgtgg agaacgccat aaagaccatg
     1921 gacgggaagg cggcctgcct gttcttcgca gacgaagatg gcgcaagcga cgtcttcgtc
     1981 ccccttgccc agaagggcct ctcggaaacc tacctccacg ccgagcccca ggaggccagg
     2041 cgcgcggccc aggagatcat ggccaggggg tacatcgcca tacacgacgc cacgaaggat
     2101 cctcgcatca agacgcacga gcagaagaag aaagagggca tcgcctcgat cctcgtggtt
     2161 cccgtggtgg tcaaggatat ggtcaagggg gtgctcgccc tgtacacctc gaagccccgg
     2221 atcttctccc aggacgagat ccgcttcctc acggcgctgg ccgaacaggg agggctcgcg
     2281 atcgagcgcg cgaggctcgt catgcagatc acccggaaca tccgcatctt cacgagggtg
     2341 gccgagggtc tcaacgagag cctcgacatc aagaagatcc tcgagaggct ctccgtggac
     2401 atgaggcagg cgctcggggt caaggcggtg tccgtacggc tcgtgaacaa ggacaccggg
     2461 accctcgagc tcgtggccag caacgggctg agcaggaaat acctggcgaa gggcccggta
     2521 cggatcgaaa agagcgtcac ggctgccctc caaggcagga ccgtggtgac gagggacgca
     2581 accaaggaca aggacatcca gttcaaggag gagaagaaga aggaaggtat cgtctccatc
     2641 ctgaccgtgc ccatcaaggt caaggacgag gtcatcggcg tcatgaggta ctactccggc
     2701 accgagcgag acttcaccga gggcgaggtc atgctcgcgg aagcggtggc gagcctgggc
     2761 gggctcgcca tacacaatgc gtccatgtac ctcatgctca agaacgacct gcaatgcctc
     2821 aaggaagatc tgtggagcca ccgctcgtgg ttctgaggcg aacgagggaa gcacgggggg
     2881 ccctgtctgc atgggcatcc ccgggggagg atcccgtcat gacaggtcga tcgataaccg
     2941 agctcaaggt cggtgacttc gccgagttcg cgcgggaccg tcgtcgcgta acagcccggc
     3001 gg
//
LOCUS       DAOSCR010000334         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21138_length_3000_cov_3.184380, whole genome shotgun sequence.
ACCESSION   DAOSCR010000334 DAOSCR010000000
VERSION     DAOSCR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21138_length_3000_cov_3.184380"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(535..1806)
                     /locus_tag="PLT69_08465"
     CDS             complement(535..1806)
                     /locus_tag="PLT69_08465"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ14508.1"
                     /translation="MASQKDFERALALGRPPTIARLFPHSKALIVSGKVIDRAMIKKG
                     KAMTIAANARNSIVIRGVLQAAQRANAAIILEIAKSEAGYCLINMWNLARYADTICNE
                     LGITVPVAIHADHFGLKKQPEVAKAHTDIPSMFDAGITSIAIDASHMPDDLNLMANLE
                     LSRHIPAWAGYETEVGEIKGKEGLSTPEEALFLIRGLNAHGVFPDWIALNNGTTHGIE
                     ATDSGIDVDLTRRIHEALAPYGISGAQHGTSGNHYERLKRIAEETRTTKANVATALQM
                     ISWGVKVNEFGNAVLDADKNFIKLSDQGVTDELWAEMVAYAQSKSLKAGDYKNLNLPF
                     ENKLLAQPREIRERMAKGVEDFVYDLLVKWFNASDTADLAIEAILEKGSYDMGPKASR
                     IEDPSEWTKEKIAEKAARLGKRQASGGNFDD"
     gene            complement(1849..2868)
                     /gene="fbp"
                     /locus_tag="PLT69_08470"
     CDS             complement(1849..2868)
                     /gene="fbp"
                     /locus_tag="PLT69_08470"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOJ14509.1"
                     /translation="MNDKIISLDRHIIEEQHRYSGRGDFSAILSQIVFAAKVVAAQVA
                     HAGIVEDILGKTGSKNIQGEEVQKLDVYANRLFINALNYIGKVCVMASEEEKDMILIP
                     PEFPRGDYVVMFDPLDGSTNIDVNVSIGTIFGIYRRVSPPGGDGTLEDCLQPGRKLFG
                     AGYVIYSSSTILVYTTGNGVNGFSLDPSVGEFILSHPNITMPRRGKIYSTNEGNYPYW
                     KKGTREYIDYLKSDRNTKKEPYTSRYIGSLVADFHRNLLKGGIFLYPEDTKDPKKPTG
                     KLRLLYEANPLAFIAEQAGGYASTGYEPILDIKPTSLHQRVPLIIGSKDDVLEYEEFA
                     KKNNE"
BASE COUNT          441 a          921 c          983 g          655 t
ORIGIN      
        1 cgacaagcgt cgcttccctg tattcgcgca ccgtgtccag ggttcgagag gctggttcgt
       61 cgggctcgga caggggcatg gccgaacgac ccggcctgtc ggatcggtgg cgcagggccc
      121 ggggagagag tgccgactgg agcatgcagg ggccgtgccc tgcaacgaga gccggtcagg
      181 ccgcctccgc gagggaggcg atctcaggca gggcatgggc aaggctgtcc tttccgggtg
      241 cgggacggac aggcttccct gccgcctgtg gcgtgcgacc gcggcccctg ttccgcgtag
      301 ggcgatcgtg ctcgtcaccc tggatagaag ggcggccggc ccgtgtggcg cccctcacgc
      361 ccggcccccg gccccggtac ccatgggtgc ctcgccaatg gcctgcgccc ggagcagaga
      421 gaggagttga ccctgccccg gtacccatgg gtgtctcacg ggtcggggcc ccacgccggg
      481 ggccttccgt ccggccatgc acggccttcg gccatgtccg gcagccttgc ggcctcagtc
      541 gtcgaagttg ccgcccgagg cctgcctctt tcccagccgg gcagccttct cggcgatctt
      601 ctccttcgtc cactctgagg ggtcttcgat cctcgacgcc tttggcccca tgtcgtacga
      661 gcccttctcg aggatcgcct cgatcgccag gtctgcggtg tccgaggcgt tgaaccactt
      721 cacgagcagg tcgtagacga agtcctccac gccctttgcc atacgctccc tgatctctct
      781 cggctgggcc aggagcttgt tctcgaaggg caggttcagg ttcttgtagt cgcccgcctt
      841 gagggacttg gactgggcgt aggccaccat ctccgcccag agctcgtccg tcaccccctg
      901 gtcggaaagc ttgatgaagt tcttgtccgc atcgagcacg gcgttgccga actcgttcac
      961 cttgaccccc caggagatca tctggagggc ggtcgccacg ttcgccttgg tggtgcgggt
     1021 ctcctcggcg atgcgcttga gcctctcgta gtggttaccc gaggtgccgt gctgcgcgcc
     1081 ggatatgccg tacggcgcga gcgcctcgtg gatgcgcctc gtgagatcca cgtcgatgcc
     1141 cgagtcggtc gcctctatcc cgtgcgtggt gccgttgttc agggcgatcc agtccgggaa
     1201 gacgccgtgg gcgttcaagc ccctgatcag gaagagggcc tcctcggggg tggacaagcc
     1261 ttccttgccc ttgatctcgc cgacctcggt ctcgtacccg gcccaggcgg ggatgtgcct
     1321 gctcagctcg aggttggcca tgaggttgag atcgtctggc atgtgggagg cgtcgatggc
     1381 gatcgacgtg atccccgcat cgaacatgga ggggatgtcg gtgtgggcct tggcaacctc
     1441 cggctgcttc ttgagcccga agtgatcggc gtggatggcc acgggcaccg tgatgcccag
     1501 ctcgttgcag atggtgtccg cgtaccgtgc caggttccac atgttgatga ggcagtagcc
     1561 ggcctccgac ttggcgatct ccaggatgat cgcggcgttc gccctctggg ccgcctgcag
     1621 gacccccctg atgacgatgc tgttgcgggc gttggccgca atggtcatgg ccttgccctt
     1681 cttgatcatg gctcggtcga taaccttgcc gctcacgatg agggccttgg aatggggaaa
     1741 gagcctcgct atcgtggggg gacgccccaa cgccagagcc cgttcgaagt ccttctgact
     1801 agccatgtct gggcctcctc gggtatatgg tttggtgttt cgcctgtgct actcgttgtt
     1861 cttcttcgcg aactcctcgt attcgagcac gtcgtccttg gagccgatga tgaggggcac
     1921 cctctggtgc aatgacgtgg gcttgatgtc gaggatgggt tcgtagccgg tggatgcgta
     1981 accacccgcc tgctcggcta tgaacgccag ggggttcgcc tcgtagagga gcctgagctt
     2041 gcccgtgggc ttcttggggt ccttggtgtc ctcggggtaa aggaagatgc cgcccttgag
     2101 gaggttccgg tggaagtccg ccacgagcga gccgatgtac cgggaggtgt agggctcctt
     2161 cttcgtgttc ctgtccgact tcaggtagtc tatgtattcc ctcgtgccct tcttccagta
     2221 cgggtagttg ccctcgttgg tgctgtatat cttgccccgc ctcggcatgg tgatgttcgg
     2281 gtgggagagg atgaactcgc ccacgctcgg gtcgaggctg aacccgttga cgccgttgcc
     2341 cgtggtatag acgaggatgg tgctcgagct gtagatgacg tagcccgccc cgaagagctt
     2401 tcgcccgggc tgcaggcagt cttccagcgt gccgtcgcct cccgggggcg ataccctgcg
     2461 gtagatgccg aagatggtcc cgatggagac gttcacgtcg atgttcgtcg aaccgtccag
     2521 ggggtcaaac atgacaacgt aatctcccct gggaaattca ggaggtatga ggatcatgtc
     2581 cttctcttcc tcggaggcca tgacgcacac cttgccgatg tagttgagtg cgttgatgaa
     2641 gagcctgttc gcgtagacgt ccagcttctg cacctcctcg ccctggatgt tcttgctgcc
     2701 ggtcttcccg aggatgtcct ccacgatccc tgcgtgggcc acctgggccg cgacgacctt
     2761 ggctgcgaac acgatctggc tgaggatggc gctgaagtcg cccctgccgc tgtaacggtg
     2821 ctgttcctct atgatgtgac ggtccaagga aatgatctta tcgttcatag agcccccttg
     2881 cgtggtgtgg tgagaacgac aggcgaatcg aacacgtgtc atgatatcca tcccgaaccc
     2941 gaaaatcaac ccgttctcgt gcggcttgcc agggcgtcgc cgaggggctt tgatggtgtt
//
LOCUS       DAOSCR010000335         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21159_length_2998_cov_3.904859, whole genome shotgun sequence.
ACCESSION   DAOSCR010000335 DAOSCR010000000
VERSION     DAOSCR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21159_length_2998_cov_3.904859"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="PLT69_08475"
     CDS             complement(<1..2266)
                     /locus_tag="PLT69_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ14510.1"
                     /translation="MAVTAESLKELEKKQAWWWIAEKKRSRRLDYLRKAVWKKGAIGG
                     LYPAGLIVDLERPVLQTEKARELESSADPYVIKFAKILAHVLDNKTIFITDHSQLVGY
                     LGSTPNTITWDPSLASILNHEVINDPQCLPEPLDDSLRTIKDIVNYWAGKSDLDKILP
                     YMDLDDSMKILSGAIGWGVPLARGGYSGKDYEYIMTGKRAFEDIIAELDRRIDDAYAK
                     AHEPSASKEISNLYDKINNWEAMKITLEAGIRYAKRYARLARIIAENFETDPARREEL
                     LKIARTCEHVPAKPPRDLQESLQYDLFIQLFSRNEAIEGAWPARPDYYHGPYYEKDVN
                     VDRVLTEEEALDLVGEFLIRAYEVSQYKPKWSREGLQGIEGTWVWTLGGVKPDGSDAC
                     NGMTIALLKAARLVRVSNPTFAFRWHPKVSDEVMRECFECIRQGLGYPSMRNDPVLVA
                     NAMHWHGHPIEEARTWVHQACMSPAPTTKHGFQPMRMANATANTAKIIEYVFTSGYDN
                     IVNMQIGAETPPVETFKSYEEFFQAWVTQMKHVFSLVVRPVNRGRMLAPTLTPRPFLS
                     AVSERSVESGLDVNDPSISRGNAWITAFTWVENIDSLAAVKKLVFDEKKYTLAELKEA
                     LANNWEGREEMRLDFVRNAPKWGNDDDYVDSIMLDCLREAAKFSRELKCPSGNSWPIL
                     PENVSGNIHYANVVGALPNGRRLGDALYDGGISPGPGLDKKGPTAVLNSCAKIDHITH
                     GRAFLLNQRLSPTQM"
     gene            complement(2319..2483)
                     /locus_tag="PLT69_08480"
     CDS             complement(2319..2483)
                     /locus_tag="PLT69_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzylsuccinate synthase gamma subunit family
                     protein"
                     /protein_id="HOJ14511.1"
                     /translation="MPTCKECKKFFPLEEDPKKGDCVQRVVDPRQAFYQAKPVEADRN
                     AASCPSFEKR"
     gene            complement(2558..>2998)
                     /locus_tag="PLT69_08485"
     CDS             complement(2558..>2998)
                     /locus_tag="PLT69_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOJ14512.1"
                     /translation="LFESIVKRAGIVLFDLKHLDQAPLEKLTGADSRLILGNLERIAA
                     RKTEIWVRIPVIPGFNDDLEFHQRASAYLSRLGESIRRVDLLPFHNWCQDKYSWLGID
                     WVYRDTEALEPSFLEVHAEWYRRRGLKVTIGGSGFEAAQEGQAA"
BASE COUNT          456 a          981 c          891 g          670 t
ORIGIN      
        1 ccatttgtgt cggggacagt ctctggttca ggaggaaggc cctgccgtgc gtgatgtgat
       61 cgatcttggc gcacgagttc aacacggcgg tcggcccctt cttgtcgagt cccggcccgg
      121 gggatatccc gccgtcgtac agggcgtcgc ccagcctccg tccattgggc agcgccccca
      181 ccacgttcgc gtagtggatg ttcccgctga cgttctcggg caggatgggc cacgagtttc
      241 cgctcgggca cttgagctcc ctggagaact tcgccgcctc gcgcaggcaa tcgagcatga
      301 tgctgtcgac gtaatcgtcg tcgttgcccc acttgggcgc gttcctgacg aaatcgagcc
      361 tcatctcctc ccttccctcc cagttgttcg cgagggcctc cttcagctcg gccaaggtgt
      421 acttcttctc gtcgaacacg agcttcttca ccgcggccag gctgtctatg ttctcgaccc
      481 acgtgaaggc cgtgatccat gcattgcccc tgctgatcga cgggtcgttc acgtcgagcc
      541 cgctctccac cgacctttcg gacacggcgg acaagaacgg cctcggtgtg agcgtcggag
      601 cgagcatgcg ccccctgttc accgggcgga cgaccaggct gaagacgtgc ttcatctggg
      661 tgacccatgc ctggaagaac tcctcgtagc tcttgaaggt ctccacgggc ggcgtctcgg
      721 cgccgatctg catgttcacg atgttgtcat acccgctggt gaacacgtac tcgatgatct
      781 tcgcggtgtt cgccgtcgcg ttggccatgc gcatgggctg gaacccgtgt ttcgtggtgg
      841 gcgcagggga catgcacgcc tggtgcaccc aggtcctcgc ctcctcgatg gggtggccgt
      901 gccagtgcat ggcgttggcg acgagaacgg ggtcgttgcg catcgagggg tagccgagac
      961 cctgcctgat gcactcgaag cactctctca tgacctcgtc gctcaccttc gggtgccatc
     1021 tgaaggcgaa cgtcgggttc gagaccctga cgagacgcgc ggccttgagg agcgcaatgg
     1081 tcatgccgtt gcacgcgtcg ctcccgtccg gcttcacgcc gcccagcgtc cacacccatg
     1141 tgccctcgat cccctggagc ccttcccgcg accatttcgg cttgtactgg gatacctcgt
     1201 aggcgcggat gaggaactcc ccgacgaggt cgagcgcctc ctcctcggtg aggaccctgt
     1261 ccacgttcac gtccttctcg tagtacgggc cgtggtagta gtcgggcctc gcgggccagg
     1321 ccccctcgat cgcctcgttc ctgctgaaga gctggatgaa gaggtcgtac tggagagatt
     1381 cctggaggtc cctgggcggc ttggccggga cgtgttcgca ggtgcgcgcg atcttcagga
     1441 gctcctccct tctcgccggg tcggtctcga agttctcggc tatgatgcgg gcgagccgtg
     1501 cgtaacgctt ggcgtacctg atgccggcct cgagggtgat cttcatggcc tcccagttgt
     1561 tgatcttgtc gtagaggttg ctgatctcct tggacgcgct gggctcgtgg gccttggcat
     1621 aggcgtcgtc tatcctgcga tcgagctcgg cgatgatgtc ctcgaaggcc cgcttgcccg
     1681 tcatgatgta ctcgtagtcc ttccccgagt atccacccct ggcgagcggc accccccacc
     1741 ctatggcacc cgagagtatc ttcatggagt cgtccaggtc catgtacggc aggatcttgt
     1801 ccaggtccga cttcccggcc cagtagttca cgatgtcctt gatcgtcctc agactgtcgt
     1861 caagcggttc cggcaggcac tgcggatcgt tgatgacctc gtgattcaag atggaagcaa
     1921 ggctcgggtc ccaggtgatc gtgttcggcg tgctgcccag gtaccccacg agctgggagt
     1981 ggtcggtgat gaagatggtc ttgttgtcga ggacgtgagc gaggatcttc gcgaacttga
     2041 tgacgtaggg gtccgcagac gactcgagtt ccctcgcctt ttcggtctgc agcacgggcc
     2101 gctcgaggtc gacgatcagc cccgccgggt acaacccgcc gatcgccccc ttcttccaca
     2161 cggccttcct gagatagtcg agcctcctgg aacgcttctt ttcagcgatc caccaccatg
     2221 cctgtttctt ttcgagttcc tttaacgatt cagctgtcac cgccatggtt cgttctcctt
     2281 gtctccttgt cccttgcccc cgtctttcag cccgcacgtc acctcttctc gaaagaaggg
     2341 cacgaggcgg cgttcctgtc cgcttccacg ggcttggcct ggtagaaggc ctgcctggga
     2401 tcgacgaccc tctgcacgca atcgcccttc ttcgggtcct cttccagtgg gaagaacttc
     2461 ttgcactcct tgcacgtcgg catgctcact cacccccttt cctgcgcgct tgacgctcga
     2521 tcttctcttg gcataacccc gtagaccccc tcgcacctca tgcagcctgc ccctcctgag
     2581 cggcctcgaa ccccgagccc ccgatggtga ccttcagtcc gcgcctccgg taccattccg
     2641 cgtgcacctc gaggaacgac ggttccaggg cctcggtgtc cctgtagacc cagtcgatgc
     2701 cgagccagga gtacttgtcc tggcaccagt tgtggaacgg gaggagatcg acccgccgta
     2761 tcgattcccc cagcctcgag aggtaggcgg aagccctctg gtgaaactcc aggtcgtcgt
     2821 tgaacccggg gatcaccggt atcctcaccc agatctcggt cttgcgtgcg gcgatgcgct
     2881 cgaggttgcc caggatgagc ctgctgtctg cgcccgtgag tttctcgagg ggggcctgat
     2941 cgaggtgctt gaggtcgaac aaaacgatac ccgctctctt cacgatggat tcgaagag
//
LOCUS       DAOSCR010000336         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21266_length_2988_cov_3.672008, whole genome shotgun sequence.
ACCESSION   DAOSCR010000336 DAOSCR010000000
VERSION     DAOSCR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21266_length_2988_cov_3.672008"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLT69_08490"
     CDS             complement(<1..838)
                     /locus_tag="PLT69_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ14513.1"
                     /translation="MTVKEASRLLKINEKKVYALVKQGRLPGTKATGKWLFPRGELEE
                     YIRRMALGSLRKSFWESLAQKKVILVCGSDDPVIAMAQGLFHGVEPDLLLFSTSVGSL
                     EGLRMLGDGFCTAATCHLFDHEAGDFTFPFLGEYFHDPQEVVVINLFHRNIGFASRGV
                     AVSSFKDALGMDLRFVNRQRGSGIRALVDHLLEEEAVDPARLTGYDTEVCTHFDAVRR
                     VVDGSADFAVASEFVAAQSRLPFTRIFEERFDLVVKRDDFFDRHVQAFVEFIRSEPFK
                     NLV"
     gene            complement(1633..2079)
                     /locus_tag="PLT69_08495"
     CDS             complement(1633..2079)
                     /locus_tag="PLT69_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14514.1"
                     /translation="MASKLMEARLAQKAYWEKTLEARIESLKEAGADETTQQKDPVVR
                     KIKARIRETASRLRAIAAKEEKIKEMARIREEKRLAALESKKEKKKTAEPEEPKKGKK
                     AKPEQQAKAPKEQKKAKAPKEPKAPKAEEEAKAPEGAAPESPDAEA"
     gene            2317..2928
                     /gene="wrbA"
                     /locus_tag="PLT69_08500"
     CDS             2317..2928
                     /gene="wrbA"
                     /locus_tag="PLT69_08500"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOJ14515.1"
                     /translation="MKALVLFYSMYGHIYQMAQAAVKGIHEVEGAEVHLKRVPETLPD
                     EVLAKMGALEARKAFEHVEVCTVAELAEYDLLVFGAPTRFGNMCGQMRQFLDATGGLW
                     ARRALVGKVGCVFTSTGTQHGGQESTILSFHLTLLHHGMVVVGLPYSFELQSDMSEIT
                     GCSPYGASTIAGPDGKRMPSTHELTGARYLARHAATIAKKLWG"
BASE COUNT          507 a          958 c          901 g          622 t
ORIGIN      
        1 ggaccaggtt cttgaacggt tcgctcctga tgaactccac gaaggcctgg acatggcggt
       61 cgaagaagtc atccctcttc acgacgaggt cgaagcgctc ctcgaagatc cgggtgaagg
      121 gtagcctcga ctgggcggcc acgaactcgg aggccaccgc gaagtccgca gagccgtcca
      181 cgacgcggcg taccgcgtcg aagtgagtac acacctcggt gtcataacct gtgagccgcg
      241 ccgggtccac ggcctcctct tcgaggagat ggtccaccag ggccctgatg cccgaacccc
      301 tctggcggtt cacgaaccgc aggtccatcc ccaatgcgtc cttgaacgag gaaacggcga
      361 cacccctcga tgcgaaaccg atgttccggt ggaacaggtt gatcaccacc acctcctgcg
      421 ggtcatggaa atactcgccg aggaagggga aggtgaaatc ccctgcctcg tggtcgaaga
      481 gatggcacgt ggccgccgtg cagaacccgt caccgagcat cctgagcccc tccaggctcc
      541 ccacgctcgt cgagaacagg agaagatccg gctccacgcc gtggaagagc ccctgggcca
      601 tggcgatgac cgggtcgtcc gagccgcaca cgaggatcac cttcttctga gcgagcgact
      661 cccagaagga cttcctcaac gatcccaatg ccatgcgcct gatgtattcc tcgagctcgc
      721 cccgcggaaa gagccacttc ccggtcgcct tggtgcccgg cagtctcccc tgcttcacga
      781 gggcgtagac cttcttctcg ttgatcttga ggagcctgga tgcctctttg acagtcaata
      841 actctttatt ttccattttc attttcaatg atgaaaatac taaccaataa tgacttatat
      901 gatgaccagc cccttgtcaa gtcatgcctg cctggcgtcc tgcacctgct ggataggctc
      961 gttctcatga ccgttgcgac aggatcttca gccccagcct gccaggccat gcccgaggta
     1021 cggtgtgcgg tctgagaccg ggaatgtctc ccggtcctaa ggggtggtct cgagaaaggg
     1081 catgcggcct cccggtacca cctcaaggcc gaggcgatgt cggcctttga caattgcata
     1141 caggtagatg cctttccaag agacctcccg atgctgccac acggccgatg cgatgtcggc
     1201 cctcgcgatg ggccgtgccg cgcttatcga gagtggttca gccggtcagg ccaggcggca
     1261 cccgctccga gaggacttca gggatgatca atgtgcgaac agccctttct cgaggtgagg
     1321 ccatggggaa accggctgag aagtatgcgt cacggatgat cgatgtgcga acagcccttt
     1381 tcacccgaag cgaggagggt cttcaaggcc gttcatgacg gcgcgagctc cgaccggtcg
     1441 acgatcatct gcgggggcat ggcgagagaa agagcggtcg cgacggggtc gtgcctgtcg
     1501 ttcggccccg gacgcggtca tagatccggg tgatcgtgac gcacgatata ctgctcggta
     1561 ctgccggacg atatctcttg gggtcgatcc aggcggcggt tgcacggccg gctgccggat
     1621 agaccctccc cttcatgcct cggcgtcggg gctttccggc gcggcacctt ctggggcctt
     1681 tgcctcctcc tcggctttgg gggctttcgg ttccttcggg gccttggcct tcttctgctc
     1741 cttgggcgcc tttgcctgtt gctcgggctt ggccttcttt cccttcttgg gctcctcagg
     1801 ttcggccgtc ttcttctttt ccttcttcga ctcgagcgcg gcgagcctct tctcttccct
     1861 gatcctcgcc atttccttta tcttctcttc cttggcggca atggcccgca gcctggaggc
     1921 cgtctccctg atcctggcct tgatcttgcg cacgacaggg tccttctgct gggtcgtctc
     1981 gtcagcccct gcctccttga gggactcgat ccgcgcctcg agggttttct cccagtatgc
     2041 cttctgggca agacgtgctt ccatgagctt ggatgccata ggagctcctt tcacctggat
     2101 acgtgttgtg taccggcctt caaagcacac aatggcatgc cttggcaagg ttttcgtgtg
     2161 gctcctgatg gcgggccgcg ccccgcccgc cgtggtctcg gccctgcccg tcggccttgt
     2221 tttcgatggc agcgggctta ttatctttcc tcacctggat gcgccccagg caggctcttt
     2281 gcctccgggc gcaaccatcg gcgaggaggt gttgtcatga aggcattggt tctcttctac
     2341 tccatgtacg gccacatcta ccagatggcc caggcggcgg tgaaagggat acacgaggtg
     2401 gagggcgccg aggtacacct caagcgcgta cccgagaccc tgccggacga ggtcctggcg
     2461 aagatgggcg ccctcgaggc ccggaaggcc ttcgaacacg tcgaggtatg caccgtcgcc
     2521 gaactcgcgg agtacgacct cctcgtgttc ggcgccccca cccgcttcgg caacatgtgc
     2581 ggccagatgc ggcagttctt ggatgccaca gggggcctgt gggccaggcg agccctcgtg
     2641 ggaaaggtgg gatgcgtctt cacgtccacc ggcacccagc atgggggcca ggaatccacg
     2701 atcttgagct tccacctcac gctcctgcac cacggcatgg tggtggtcgg ccttccctat
     2761 tccttcgagc tgcagtcgga tatgagcgag atcacggggt gttctcccta cggcgcatcc
     2821 accatcgcag ggccggacgg caagaggatg ccgagcaccc acgagctcac gggggcccgc
     2881 tacctcgcac ggcacgcggc gaccatcgcc aagaaactct ggggatgagc ccgggggctc
     2941 gcccctgaga aatcgccggg gtaggccttg atcgggtggg gtcgcccc
//
LOCUS       DAOSCR010000337         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21365_length_2980_cov_2.329573, whole genome shotgun sequence.
ACCESSION   DAOSCR010000337 DAOSCR010000000
VERSION     DAOSCR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21365_length_2980_cov_2.329573"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1012)
                     /locus_tag="PLT69_08505"
     CDS             complement(101..1012)
                     /locus_tag="PLT69_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877725.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOJ14516.1"
                     /translation="MTPPRYRTFSAWAREHFASKARKITLDAGLTCPHRDSRGRGGCI
                     YCNDRGSGTGAHARGLSVADQLELGIRRLSSPGRKTVFIAYFQSFTNTYAPPETLSSI
                     YDEVLSFPEVAGLSIGTRPDCIDEEKLGLIAGYTDRLTVWVEYGLQSASDETLRLINR
                     GHDVKTFVDAVELTSRFGLRICAHAIIGLPGEGMDHYLKTARLLSLLPVTDVKIHLLY
                     VVKGTPLEDLFRRGAYRPLEPAEYAEAVARFIGNLREDIVIQRITGDPHPSELVSPSW
                     ALDKAMVRSIVHETMDRLGILQGSLRG"
     gene            complement(969..2120)
                     /locus_tag="PLT69_08510"
     CDS             complement(969..2120)
                     /locus_tag="PLT69_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOJ14517.1"
                     /translation="MSHDTVYVDHRELRRFMREGFVAMGVPSDEADICADVLIESDLR
                     GIKSHGIGRFKMYIDRMKDGIISPVCTMRIVSETENTAVVDGGDSLGHVVAKRAMQLA
                     IDKAEKHHVGMVAVRNSTHFGICGYYPSMAVERDMVGLAFSNARPSVAPTFGVEPVLG
                     TNPISFGAPSDEVYPFLLDIATSTTQRGKIEVYALEGSPTPKGLTIDIHGNQPTDSKA
                     LLKMFVDGTASLLPLGGAGEELGGHKGYGLGVMVEILCAAFCGGAFGKALSGFEDGRP
                     APHRLGHFFAAINIASFVPVGVFKAITGSIMREIRASAKAPGHDRIFIAGEKEFEMVE
                     RRKKEGVPIPRPLQEIMIQLKHELGLEGFPFPFFADDTATVQDVQRMGA"
     gene            complement(2502..>2980)
                     /locus_tag="PLT69_08515"
     CDS             complement(2502..>2980)
                     /locus_tag="PLT69_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ14518.1"
                     /translation="GIVGLDTQDRRLVSEPVWHDELVLVSKPGHPLSTCGRATMETVL
                     GHPFVMREKGSGTRKVFERYVSDVFGLGIDRFHVVAEMGSTEAVKESVMAGLGVSVVS
                     IHSIRRELSSGVLARIQVEGMDIHRSFHLVRRRHSVRLPHHDAFIEVCRAYDVGGG"
BASE COUNT          493 a          953 c          975 g          559 t
ORIGIN      
        1 cctgttccct gcagagcatg cgcatgggcc agctcgtgta tcccgacagc ggggcgagga
       61 agagggctgc cttgaagacc atgccgtagg cggtcaaggt tcacccccgg aggcttccct
      121 gcaggatgcc gagcctgtcc atggtctcgt ggacgatgga gcggaccatg gccttgtcga
      181 gggcccacga cggcgagacg agctcggatg ggtgggggtc gccggtgatg cgctggatca
      241 cgatgtcctc cctcaagttg ccgatgaagc gcgcgaccgc ctccgcgtac tccgccggtt
      301 cgagcggcct gtatgcgccc ctcctgaaca ggtcctcgag gggcgtgccc ttgaccacgt
      361 agaggaggtg tatcttgacg tcggtcacgg gcaggagaga caggaggcgg gcggtcttca
      421 ggtagtgatc catcccctcg ccgggcagcc ctatgatcgc gtgggcgcat atcctcaggc
      481 cgaacctcga ggtgagctcc accgcgtcga cgaaggtctt cacgtcgtgg ccgcggttga
      541 tgaggcggag cgtctcgtcg ctcgccgact ggaggccgta ctccacccac accgtgagcc
      601 tgtccgtgta tcccgctatg agcccgagct tctcctcgtc tatgcagtcg ggcctggtgc
      661 ctatggacag gcccgccacc tcgggaaagg acagcacctc gtcgtagatg gatgagagcg
      721 tctccggcgg ggcatacgtg ttggtgaaag actggaagta tgcgatgaag accgttttcc
      781 tgccgggcga ggagagcctg cggatgccga gctcgagttg gtccgccacg ctcaggcccc
      841 gcgcgtgcgc cccggttccc gaacccctgt cgttgcagta gatgcagccg cccctgccgc
      901 ggctgtcccg gtgcgggcac gtcaggcccg catccagggt gatcttgcgg gccttcgagg
      961 cgaagtgctc acgcgcccat gcgctgaacg tcctgtaccg tggcggtgtc atccgcgaag
     1021 aaagggaacg ggaaaccctc gagcccgagc tcatgcttga gctggatcat gatctcctgg
     1081 agaggcctcg ggatggggac gccctccttc ttgcggcgct cgaccatctc gaactccttc
     1141 tcgcccgcga tgaagatcct gtcgtggccc ggtgccttcg ccgaggcccg tatctcgcgc
     1201 atgatggacc cggtgatggc cttgaacacc cctaccggca cgaagctggc gatgtttatg
     1261 gctgcgaaga agtggccgag cctgtggggt gccgggcggc cgtcttcgaa gccggagagc
     1321 gccttgccga acgcgccccc gcagaaggcc gcacacagga tctccaccat gacgccgagg
     1381 ccgtagccct tgtggccgcc cagttcctcg cccgcgccgc ccagggggag cagcgaggcc
     1441 gtcccgtcca cgaacatctt gaggagcgcc ttgctgtccg tcggctggtt gccgtggatg
     1501 tcaatggtca accccttggg ggtgggggag ccttccagcg cgtagacctc tatcttgccc
     1561 ctctgggtgg tggaggtggc gatatcgagg aggaaggggt agacctcgtc cgaaggcgcc
     1621 ccgaagctga tggggttggt gcccaggacc ggttccacgc cgaaggtggg agcgaccgac
     1681 ggccgtgcgt tggagaaggc caaccccacc atgtcccgct cgacggccat ggaggggtag
     1741 tacccgcaga tgccgaagtg ggtgctgttc ctgacggcca ccattcccac gtggtgcttt
     1801 tccgccttgt ctatggcgag ctgcatggcc cttttggcga ccacgtgacc gagactgtcc
     1861 cccccgtcga cgacggccgt gttttccgtc tcgctcacga tgcgcatggt gcagaccggg
     1921 gatatgatcc cgtccttcat ccggtcgatg tacatcttga acctgccgat gccgtgggac
     1981 ttgatgcccc tgaggtccga ctcgatgagc acgtcggcgc agatgtccgc ctcgtcgctc
     2041 gggaccccca tggcgacgaa gccttccctc atgaatctcc tgagttccct gtgatctacg
     2101 tacaccgtgt cgtgtgacat ggccgtttca cctgcttctt tcgttggata gatgcgagac
     2161 ccccgtctcg cggttctctt agcagatggc ccttgtttcg gaaaggagaa aatgacaccg
     2221 aaggacctcc ctctccgatc gctcttttcc cgacttccgg gggcaggagg ggacgggacg
     2281 gcctctcggg ggacccgtgg ccgttttctt tctcgtccca ggggggatgt gggagatcct
     2341 gaaccgcgag ataccggaca cagcctctcg tcccgcgggg ggatgtggtc ttctgaaggg
     2401 atgggctgac cacggatgga ccctctccac acgcgccccg gcccgagtga aaggtcaacg
     2461 agagcttgag gaccccggga agcggctcgg gcttgccttc ctcagccgcc gccgacgtcg
     2521 tacgccctgc acacctcgat gaaggcatcg tgatgaggga gacggacgga gtgtctcctc
     2581 ctgacgaggt ggaacgaccg gtggatatcc atgccctcga cctgtatgcg tgcgagaacg
     2641 cccgaggaga gttcgcgccg tatcgagtgg atggacacca cggagacgcc gagacctgcc
     2701 atgaccgact ccttgaccgc ctcggtggac cccatctccg cgacgacgtg gaacctgtcg
     2761 atccccaggc cgaagacatc cgagacgtac ctctcgaaca ccttccgggt gcccgaaccc
     2821 ttctccctca tgacgaacgg gtgtcccagc accgtctcca tggtggcgcg gccgcacgtg
     2881 gagaggggat ggccgggttt tgagacgagg acgagctcgt cgtgccatac aggctcggag
     2941 acgagcctcc tgtcttgcgt gtcaaggccc acgatcccga
//
LOCUS       DAOSCR010000338         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21373_length_2979_cov_2.774282, whole genome shotgun sequence.
ACCESSION   DAOSCR010000338 DAOSCR010000000
VERSION     DAOSCR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21373_length_2979_cov_2.774282"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2979
                     /locus_tag="PLT69_08520"
     CDS             <1..>2979
                     /locus_tag="PLT69_08520"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ14519.1"
                     /translation="RTIFEIGGQDAKFIRLEAGTVVDVNMNYVCAAGTGSFVEEQART
                     LGIGLDEIGRLVMGVAPLPNSDRCTVFMNREISRQLSQGLPRERILAGVLVAVLKNYL
                     TKVVGTRRYARDRIVFQGATARNIGLVAALEQLTGASILVSPFCHVMGAFGAALLASE
                     KVGSQTRFLGFSIPEVSVTESTCRGCENSCRITHLDMDGEKTSWGYLCGREPGVRSRG
                     RRVNRCIKERNDTVSAYASRYTPGPKGTVKMPALGLNDEFTPLFGEIMQASGYGLELV
                     RVERARILGELSRLGTGDFCWPVKVSMASAMAVLSGDPSCRVLLPHLVQGRKDPSIHP
                     RSLYCPFMTALPSFLRSRPGHDRIIPLVIDLGRPMGELARLVARTLEEAGLGRVPAPR
                     LEGAMELGLALTDRMRGEVSSAARRLLEEAGPDEKVIVVIGRPYNLYHQVLNLGIPEL
                     VESLGYRVVTLDAVPDLVTDKEVCRSFPDMYWAQGLKILRKAASIVSMPNVFPLVISN
                     FSCGPDSFLLTYLEELFRDKPYLILELDEHGSATGYQTRIEAFLDMIEQHRRTSTPRA
                     GMLAGRRIRYSLKDIPEGTTVWIPQIHPYTPQLWAAVLGRKGIDARPLGEETADDCSL
                     GRELCRGSECLPAAVTTGRYAAEVRRRERAGSTCLFMPRAEGPCRFGQYATLQSLVME
                     RTGMEGSFVFSPTSENGYAFLDRSTELAVWEALCLGDALYKLRCRVVPYHDRPDAAQG
                     LIASAIDEICTAMRKGGDWRGIARALPAMLDAHRYLSRPRRPLVGIVGEIFVRLNLFS
                     NQHVVETVERYGGEAWLAPMSEWVHYVWRLLRVKSPLLSKLRPMIKSYILHRVEQDVM
                     ALFRGVLDDRHEPPIGEVLARGSRLVPLAFEGEAILTLGRAQIFGEQGASLVVNCSPF
                     GCMPGRITAYLFQSMQGFFKAPVVNLFFDGTGDVAEEVGVYLTSLARGDERRSSVSSV
                     FKLPGRTSPLGVVEDEHQART"
BASE COUNT          503 a          978 c         1003 g          495 t
ORIGIN      
        1 acggaccatc ttcgagatcg gcggccagga cgcgaagttc atacggctgg aggccgggac
       61 cgtggtggac gtgaacatga actacgtctg cgccgcgggc acgggttcct tcgtggagga
      121 gcaggcccgg acgctcggca tcggcctcga cgagataggc aggctcgtca tgggcgtggc
      181 accactcccc aactccgaca ggtgcacggt cttcatgaac agggagatct cgcgccagct
      241 ctcgcagggc ctgcccaggg agagaatcct ggcaggggtg ctggtcgcgg tgctcaagaa
      301 ctacctcacc aaggtggtgg gcacgaggcg ttacgcaagg gacaggatcg tcttccaggg
      361 cgccaccgcc aggaacatcg ggctcgttgc ggccctcgag caactcacgg gggcgagcat
      421 cctggtttcc ccgttctgcc acgtgatggg cgccttcggg gcggccctcc tggcatccga
      481 gaaggtcggg tcacagacgc gctttctggg gttctcgatc cccgaggtct cggttacgga
      541 gtccacgtgc agggggtgcg agaactcctg caggatcacg cacctcgaca tggacggcga
      601 gaagacgagc tggggatacc tctgcgggag ggaacccggg gtccgctcga ggggcaggcg
      661 cgtcaacagg tgcatcaagg aacgaaacga cacggtctcg gcatacgcct cgcgttacac
      721 ccccggcccc aaggggaccg tgaagatgcc tgcgctcggc ctcaacgacg aattcacccc
      781 cctcttcggg gagatcatgc aggcatcggg ctacgggctc gagctcgtga gggtggaacg
      841 ggccaggatc ttgggcgaac tctcgcgcct cgggacgggg gacttctgct ggccggtcaa
      901 ggtgtccatg gcgtccgcca tggccgtcct gtccggcgac ccatcctgca gggtgttgct
      961 gccgcacctc gtgcaggggc gcaaagaccc gtccatccac ccgcggagcc tctactgccc
     1021 gttcatgacc gcgctcccct ctttcctccg ctcgaggccc gggcatgaca ggatcatccc
     1081 cctggtgatc gacctgggca ggcccatggg cgagctggcc cgtctcgtcg cccgcaccct
     1141 cgaggaggcg gggctcggcc gggtacccgc ccctaggctc gagggtgcca tggagctggg
     1201 cctcgccctt acggatcgca tgaggggcga ggtgtcttcg gccgcacggc gcctgctcga
     1261 ggaagcgggc ccggacgaga aggtcatcgt ggtcatcggc aggccgtaca acctctacca
     1321 ccaggtgctg aacctcggca tacccgagct cgtggagtcc ctcgggtacc gggtggtcac
     1381 cctggacgcc gtgcccgacc tcgtgaccga caaggaggtg tgcaggtcct tccctgacat
     1441 gtactgggcg caggggctga agatcctccg aaaggccgct tccatcgtct ccatgccgaa
     1501 cgtcttcccc ctcgtgatct ccaacttctc ctgcggcccg gactcgttcc tcctgaccta
     1561 cctcgaggag ctcttcaggg acaagccgta cctcatcctc gaactcgacg agcacgggtc
     1621 cgcgaccggc taccagaccc gcatcgaggc ctttctcgac atgatcgagc agcaccgccg
     1681 gacctcgacc ccacgggcag ggatgctcgc cgggaggcgg atacgctact ccctgaagga
     1741 catccccgag ggcaccaccg tctggatccc ccagatccac ccctacacgc cgcagctgtg
     1801 ggcggcggtc ctcggaagga aggggatcga cgcgaggccc ttgggcgaag agaccgctga
     1861 tgactgctcg ctgggcaggg aactctgcag ggggagcgag tgcctcccgg ccgccgtcac
     1921 caccggaagg tacgcggccg aggtcaggcg ccgggaaagg gccgggagca cctgtctctt
     1981 catgccgagg gccgagggac cgtgccggtt cggacagtat gccacgctcc agtccctcgt
     2041 catggagcgg acgggcatgg agggctcctt cgtgttctcg cccacctcgg agaacgggta
     2101 cgccttcctc gatcgctcga ccgagcttgc cgtctgggag gccctgtgcc tgggggatgc
     2161 gctctacaag ctcaggtgcc gggtcgtccc gtaccatgac aggccagatg cggcccaagg
     2221 tctgatagcg agcgcgatcg acgagatctg cacggcgatg cgcaagggag gcgactggcg
     2281 cggtatcgct cgagcgctgc ccgccatgct ggatgcacac cggtacctct caaggccccg
     2341 caggccgctc gtgggcatcg tgggcgagat cttcgtgcgc ctcaacctct tctccaacca
     2401 gcacgtggtg gagacggtgg aacgctacgg gggcgaggct tggcttgcgc ccatgtcgga
     2461 gtgggtgcac tacgtgtggc gtctgctgcg tgtcaagtcg ccccttctgt ccaagctcag
     2521 gccgatgatc aagtcataca tcctccaccg cgtggaacaa gacgtgatgg cactcttcag
     2581 gggcgtgctc gacgacaggc acgaaccgcc gatcggggag gtcctcgcaa gggggagcag
     2641 gctggtcccg ctcgccttcg agggggaggc catcctcacc ctgggcaggg cgcagatctt
     2701 cggcgagcag ggtgcctcgc tggtggtgaa ctgctctccc ttcggctgca tgcccgggag
     2761 gatcaccgcg tacctgttcc agtccatgca agggttcttc aaggcgcccg tggtgaacct
     2821 cttcttcgac ggcacgggcg acgtggccga ggaggtgggg gtgtacctca catccctggc
     2881 gaggggggac gagaggcgat cgagcgtctc ctcggtcttc aagctccccg gccggacaag
     2941 ccccctaggc gtggtcgaag acgaacacca ggcgagaac
//
LOCUS       DAOSCR010000339         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21468_length_2969_cov_3.949554, whole genome shotgun sequence.
ACCESSION   DAOSCR010000339 DAOSCR010000000
VERSION     DAOSCR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_2969_cov_3.949554"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLT69_08525"
     CDS             <1..372
                     /locus_tag="PLT69_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOJ14520.1"
                     /translation="TVLFITGAMTIVGCIQDGTTGNHDTLFLKSMLDGVASMTLASTL
                     GIGVAFSALSVLVVQGGLTLLAGHVGVLQEPEVLGSITTTGGMIILGIGINLLGLTSI
                     RVGNLIPALVYAAVYPIVLGR"
     gene            390..968
                     /locus_tag="PLT69_08530"
     CDS             390..968
                     /locus_tag="PLT69_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOJ14521.1"
                     /translation="MSHVRGETMEGFVERDESFLLVIDVQERLHAAMDASSAQAYARN
                     CGILVRTASRLGLPVVVTEQYPKGLGPTIESLAGVLGDEPRMSKLAFSCMREEPIAAR
                     IRSIGRKRAIIAGIEAHVCVLQTAVDMMREGMRVVVASDEVCSRRASDRDAALDTLRT
                     LGALVYPTETIAFMLLERAGTDEFKDLAPLFR"
     gene            1119..2003
                     /gene="rpsB"
                     /locus_tag="PLT69_08535"
     CDS             1119..2003
                     /gene="rpsB"
                     /locus_tag="PLT69_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOJ14522.1"
                     /translation="MSIVTMKQLLEAGVHFGHQTPRWNPKMKPYIFGSRNGMHIIDLQ
                     KTEKLFEKACEVIRETVANGGKVLFVGTKKQAQDAIEEEAGRVGAFYVNYRWLGGTLT
                     NFSTIRKSVDKLKWLESIIDNGTIENYPKKEQISMKRHKMKLERSLHGIKEMNTLPDL
                     LYIVDPSREYIAVQEARKLKIPIVAIVDTNCDPTLIDYVIPGNDDAIRAIRLFTSKVA
                     DAVDQGNQIWQEKLSSEAREEALPEPAAGKAPKEEQLGKGLEGEIEIIVKDAGDEPPA
                     RGRRARAASQAEEATDEE"
     gene            2025..2624
                     /gene="tsf"
                     /locus_tag="PLT69_08540"
     CDS             2025..2624
                     /gene="tsf"
                     /locus_tag="PLT69_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ14523.1"
                     /translation="MQITSEAVKKLRDKTGVGMMDCKKALEETGGDFDKAVEILRKKG
                     IEVASKRSGRTASQGAVASYIHMGGKIGVLVEVNCESDFVAKSEQFNAFVKDIAMQIA
                     AASPDWVTREEVPAEALEKEREILREQALKTGKPANVVEKIVEGKLSKFYSERCLLDQ
                     PFVKDQGKTVRQLLDELMAKTGEKCVVRRFVRFQLGEEI"
     gene            2634..>2969
                     /gene="pyrH"
                     /locus_tag="PLT69_08545"
     CDS             2634..>2969
                     /gene="pyrH"
                     /locus_tag="PLT69_08545"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771230.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ14524.1"
                     /translation="MHYRRILLKLSGEALAGEKGFGIDTRILDEIVSRVKEATESGVS
                     VALVIGGGNFFRGTQADEALDRVTADSIGMLATVMNALAFRAALAGSGVTCCLMSALA
                     IPGVAAPVDI"
BASE COUNT          611 a          880 c         1017 g          461 t
ORIGIN      
        1 accgttctct tcatcacggg cgccatgacg atcgtggggt gcatccagga cggcacgacg
       61 ggcaaccacg acacgctctt cctcaagtcc atgctcgacg gcgtcgcgtc catgacgctg
      121 gcctcgacgc tcgggatcgg cgtggcgttc tccgccctga gtgtgctcgt ggtccagggc
      181 ggtctcacgc tgctggccgg ccacgtgggc gtgctgcagg agccggaggt gctggggtcc
      241 attaccacca ccggcggcat gatcatcctc ggcatcggga taaacctcct gggtctcacg
      301 tcgatccgcg tgggcaacct catcccggcc ctcgtctacg ccgccgtcta cccgatcgtt
      361 ctcgggcgat gaggaggacg cgcagacccg tgagccacgt gaggggggag accatggaag
      421 gcttcgtgga gagggacgag tcgttcctgc tcgtcattga cgtccaggag aggctccatg
      481 cggccatgga cgcctcttcg gcccaggcct acgcgaggaa ctgcgggatc ctcgtcagaa
      541 cggcatcgag gctggggctc ccggtggtgg tcaccgagca gtacccgaag ggcctgggcc
      601 cgaccatcga gagccttgca ggcgtcctcg gcgacgagcc gaggatgtcc aagctcgcct
      661 tcagctgcat gcgggaagag cccatagcgg cgcgcatacg ctcgataggg aggaaaaggg
      721 cgatcatcgc ggggatagag gcccacgtgt gcgttttgca gactgccgtg gacatgatgc
      781 gggagggcat gcgcgtggtg gtcgcctctg acgaggtatg ctcgcggagg gcttcggaca
      841 gggatgccgc gctggatacg ctgaggacgc tcggggcgct ggtgtacccg accgagacca
      901 tcgccttcat gctcctggaa agggcgggga ccgacgagtt caaggacctg gcgccgctgt
      961 tcaggtaagt acagcatgtg ggtcttgaaa ctcgcggatg tgtgtggtaa agcgctcaac
     1021 cagcacgcac gtcccggtgc atgccgggtt caccggtgtc cccttgacga aaggggatgt
     1081 ggcccggcgg gacggaggaa aaaccaagga ggtaacccat gtcgatcgtc accatgaagc
     1141 agctcctcga ggccggcgtt catttcggcc accagacgcc gcggtggaac cccaaaatga
     1201 aaccctacat cttcggttcg cgcaacggca tgcacatcat cgatctccag aagacggaga
     1261 agctctttga gaaggcctgc gaggtcatca gggagaccgt ggccaacggc gggaaggtcc
     1321 tgttcgtggg cacgaagaaa caggcccagg acgccatcga ggaagaggcg ggaagggtgg
     1381 gcgccttcta cgtcaactac cggtggctgg gcggcaccct cacgaacttc tccacgatcc
     1441 gcaagagcgt ggacaagctc aagtggctcg agtcgatcat cgacaacggc accatcgaga
     1501 actacccgaa gaaggaacag atcagcatga agcgccacaa gatgaagctc gagcgctccc
     1561 tgcacggcat caaggagatg aacaccctgc ccgacctgct ctacatcgtc gacccgtcgc
     1621 gggagtacat cgcggtccag gaggcgagga agctcaagat ccccatcgtg gccatcgtcg
     1681 acaccaactg cgaccccacg ctcatcgact acgtgatccc gggcaacgac gacgccatca
     1741 gggccatacg gctcttcacc tccaaggtgg cggacgccgt cgaccagggc aaccagatct
     1801 ggcaggagaa gctctcctcc gaggccaggg aggaagccct ccccgagccc gccgccggga
     1861 aggccccgaa ggaggagcag ctcggcaagg ggctcgaggg tgagatcgag atcatcgtga
     1921 aggacgcggg tgacgagccg cccgcacgcg ggagaagggc cagggccgcg tcccaggccg
     1981 aggaagcgac agacgaggaa tagttcacca cagcggaggt acgtgtgcag ataacatccg
     2041 aagcggtcaa gaagctgagg gacaagacgg gcgtcggcat gatggactgc aagaaggccc
     2101 tcgaagagac gggcggggat ttcgacaagg cggtggagat cctcaggaag aagggcatag
     2161 aggtcgcgag caagcgctcc gggagaacgg cgagccaggg ggcggtggcc tcgtacatcc
     2221 acatgggcgg caagatcggc gtgctcgtgg aggtgaattg cgagagcgat ttcgtggcca
     2281 agtccgagca gttcaacgcc ttcgtcaagg acatagcgat gcagatagcg gcggcgagcc
     2341 ccgactgggt cacccgcgag gaagtgccgg cggaggccct cgagaaggag agggagatcc
     2401 tcagggagca ggccctcaag acgggcaagc ccgccaacgt cgtcgaaaag atcgtcgagg
     2461 gcaagctctc caagttctat tccgagcggt gcctcctgga ccagcccttc gtgaaggacc
     2521 agggcaagac cgtccggcag cttctggacg agctcatggc caagaccggc gagaagtgtg
     2581 tcgtcaggcg cttcgtcagg ttccagctgg gagaagagat ctaggggtct tgcttgcact
     2641 accggcgcat tcttctcaag ctgagcggtg aggctctggc cggggagaag ggtttcggca
     2701 tcgacactcg catcctcgat gagatcgtct cccgggtgaa ggaagcgacg gaatcagggg
     2761 taagcgtcgc cctcgtcata ggcgggggga acttcttccg cgggacgcag gccgacgagg
     2821 cgctcgacag ggtgaccgcg gactccatcg gcatgctcgc cacggtcatg aacgcgctcg
     2881 cgttcagggc agcgcttgcg ggctcggggg tcacctgttg tctcatgagc gcgctcgcca
     2941 tacccggggt ggccgccccg gtcgatatc
//
LOCUS       DAOSCR010000340         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21563_length_2960_cov_3.553528, whole genome shotgun sequence.
ACCESSION   DAOSCR010000340 DAOSCR010000000
VERSION     DAOSCR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21563_length_2960_cov_3.553528"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLT69_08550"
     CDS             complement(<1..711)
                     /locus_tag="PLT69_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ14525.1"
                     /translation="MISRELKLSRGGIPIRAWLYFPEPKPTRLLDVLLFCHGIPGSKP
                     DPKDRGYLPLFEEFTARGYACATFNFRGCGVSGGNIDMRGWHEDLDAVLDAVTSCPGV
                     DPHAVHCIGFSAGGAVAAKEASYHPEVKSLLMVASPADFSEILPKDPDVLRRHFLGLG
                     LIRDESFPGDLDAWYRGFLETNPARWLPFVHPRPVGIVHGSGDETVPVRHGHLLYGAA
                     WEPKRLFVLEQAGHQLRKD"
     gene            complement(644..1783)
                     /locus_tag="PLT69_08555"
     CDS             complement(644..1783)
                     /locus_tag="PLT69_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14526.1"
                     /translation="MRGKLEHPRGLIELISVEVERIDPEYSELWLSFEYTCPGGQYKS
                     AMVLPIGNSKDMVNFFFKELIAEAKHLSEIEKNDALSVGLFVSNKTDTMLTIKNLCQR
                     VIEVLNTDEDLSENREFIDLCTSGFHFKDPSKSLSSMDFDDRYRIFMSRATEKMSQGY
                     YTIAADDLEKANILCSTSPLIHKLLGVCHRELGHLDLAIDMFNEALDLGDREKETYLY
                     LSEIYFFLNEMENAKDVLLEMLDEYPDDVRATVELANIEYQLDRDYLPLLDKAFSKDP
                     DLTKNVIMQTFVFKKVGGGKKNRIPIEEASRLVGIPAHAIRQLAQSNRIPVRAFPDND
                     ELVLDENELKAWAQVYRRYNLLADDLERTETQQGGDTHQGMALLS"
     gene            1897..2115
                     /locus_tag="PLT69_08560"
     CDS             1897..2115
                     /locus_tag="PLT69_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14527.1"
                     /translation="MRKYGCMSFSWVVAMCALILGACYSDGHAQPRAKVDKVTVDIGT
                     VLEGQDASHGFLITNEGDAPLVIKPRPC"
     gene            2125..2637
                     /locus_tag="PLT69_08565"
     CDS             2125..2637
                     /locus_tag="PLT69_08565"
                     /inference="COORDINATES: protein motif:HMM:NF027116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14528.1"
                     /translation="MAVPGGRQTEDKKTVIAPGASQKVVLTVPTKYVRSREPYHKTVE
                     VETNDPDKPVVRITLQFTVKDILVVRPDPVAFGKVRVGSENSKPLTITNKTKDAITIR
                     KVSVFPGHALNVSPNADVRVDPGKTVTVQVTFRPPSPDRSFLGLVQIETDRENLGVKT
                     VQVRASVTED"
     gene            2671..>2960
                     /locus_tag="PLT69_08570"
     CDS             2671..>2960
                     /locus_tag="PLT69_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOJ14529.1"
                     /translation="MGGGFRKDVLTVGSLTAASRVLGFVRDMVMAWLLGAGPLADAFV
                     VAFRLPNLLRRFMAEGAVSVAFVPVFQEVKERDGTDTAFVLARQVFTLMFLVL"
BASE COUNT          519 a          871 c          981 g          589 t
ORIGIN      
        1 gtccttccgc agctggtgcc cggcctgctc gagcacgaag agcctcttgg gttcccaggc
       61 cgcgccgtag agcaggtggc cgtgtctaac gggcacggtc tcgtctccgc ttccgtgcac
      121 gatgcccacc ggacgcgggt gcacgaacgg cagccatcgg gccgggttcg tctcgagaaa
      181 gcccctgtac caggcgtcga ggtctccggg gaaggattcg tcccgtatga gaccgagccc
      241 gaggaagtgt ctcctgagca cgtccgggtc tttggggagg atctcggaga agtccgcggg
      301 cgatgccacc atgaggaggc tcttgacctc cgggtggtaa gacgcctcct tggcggccac
      361 cgcgcccccc gccgagaacc ctatgcagtg cacggcgtga gggtcgacgc ccgggcacga
      421 agtgacggcg tcgagcaccg cgtccaggtc ttcgtgccag cccctcatgt ctatgttccc
      481 gcccgagacg ccgcaccccc tgaagttgaa ggtggcgcat gcataccccc gggccgtgaa
      541 ttcctcgaac aggggcagat accccctgtc cttggggtcg ggtttcgaac cggggatgcc
      601 gtggcagaag aggaggacgt cgaggagtct cgtgggcttg ggctcaggaa agtagagcca
      661 tgccctgatg ggtatccccc ccctgctgag tttcagttct ctcgagatca tcggcgagca
      721 gattgtatct cctgtatacc tgggcccagg ccttgagctc gttctcgtcg agcacgagct
      781 cgtcgttgtc cgggaaggcc ctgaccggga tcctgttcga ctgtgcgagc tgcctgatgg
      841 cgtgcgcagg gataccgacc aggcgcgagg cctcctcgat cggtatgcgg ttcttctttc
      901 cgcctccgac cttcttgaag acgaaggtct gcatgatgac gttcttggtc aggtccgggt
      961 ccttggagaa ggccttgtcg aggaggggca ggtagtccct gtcgagctgg tactcgatgt
     1021 tcgcgagctc cacggtggcc ctcacgtcgt cgggatactc gtcgagcatc tccaggagaa
     1081 catccttcgc gttctccatc tcgttcagga agaagtagat ctccgagagg tagaggtagg
     1141 tctccttctc gcggtctccg aggtcgagcg cctcgttgaa catgtcgatc gccaggtcca
     1201 ggtggccgag ttcccggtgg cagacgccaa ggagcttgtg gatgaggggg ctcgtcgagc
     1261 agaggatgtt cgccttctcc aggtcgtcgg ccgcgatcgt gtagtatccc tgcgacatct
     1321 tctccgtggc cctgctcatg aatatccggt agcggtcgtc gaagtccatg gaactcaggc
     1381 tcttcgaagg gtccttgaag tggaaaccgg aggtgcacag gtctatgaac tccctgttct
     1441 cgctcaggtc ctcgtcggtg ttgaggactt ctatgaccct ctggcagaga ttcttgatgg
     1501 tgagcatggt gtcggtcttg ttcgagacga agaggccgac gctcaacgcg tcgttcttct
     1561 cgatctcgga gaggtgcttc gcctcggcga tgagctcctt gaagaagaag ttcaccatgt
     1621 ccttcgagtt gccgatgggc agtaccatgg ccgacttgta ctggcccccc gggcaggtgt
     1681 actcgaagct cagccacagc tcgctgtact cgggatctat cctctcgacc tcgacggata
     1741 tgagctcgat gagtcctctt gggtgttcga gtttgccgcg catgacttgg atgtatgacg
     1801 tgggtgacca aaagtcaacc tgttcgaggc gtgcgggtca ggtgggtgtg gaaaaagccc
     1861 tttttctgtg atagccaaaa aacaggaggg aaaggtatga gaaagtacgg ctgcatgtct
     1921 ttctcgtggg tcgtcgccat gtgcgccctc atcctcggcg cctgctattc cgacggacat
     1981 gcccagccca gggccaaggt ggacaaggtc acggtcgaca taggcaccgt gctggaaggg
     2041 caggacgcgt cacacggttt tttgatcacg aacgaaggcg acgcaccgct cgtgatcaag
     2101 ccgaggcctt gctgaggcgc cacggtggcc gtcccgggcg gacggcagac cgaggacaag
     2161 aagaccgtga tcgcccccgg cgcatcgcag aaggtggtcc tcaccgtgcc caccaagtac
     2221 gtgcggtcgc gcgagccgta ccacaagacc gtggaggtgg agaccaacga cccggacaag
     2281 cccgtcgtga ggatcaccct gcagttcacg gtaaaggaca tcctggtcgt tcggccggac
     2341 ccggtcgcct tcgggaaggt aagggtcggg agcgagaact ccaagcccct gacgatcacg
     2401 aacaagacga aggatgcgat aacgatcagg aaggtctccg tgttcccggg gcatgccctg
     2461 aatgtgtcgc cgaacgcaga cgtgcgggtc gaccctggaa agaccgtgac ggtgcaggtg
     2521 acgttcaggc ctccatcccc ggacaggagc ttcctggggc tggtgcagat cgagaccgac
     2581 agggagaacc tgggcgtcaa gaccgtgcag gtgcgggcct cggtgaccga ggactgaggc
     2641 ggcctcgggc tcaggggacg gcggccgcgc atgggagggg ggttcaggaa ggacgtcctg
     2701 acggtcgggt cgctcacggc ggcgagcagg gtcctcggct tcgtccgcga catggtcatg
     2761 gcgtggctct tgggtgccgg cccccttgcc gacgccttcg tggtggcttt caggttgccg
     2821 aacctgctca ggcgtttcat ggccgagggc gcggtcagcg tggcgttcgt ccccgtcttc
     2881 caggaggtca aggaacgcga cgggacggac acggccttcg tgctggcccg ccaggtcttc
     2941 accctcatgt tcctcgtcct
//
LOCUS       DAOSCR010000341         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21589_length_2958_cov_2.977609, whole genome shotgun sequence.
ACCESSION   DAOSCR010000341 DAOSCR010000000
VERSION     DAOSCR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21589_length_2958_cov_2.977609"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1080..1232
                     /locus_tag="PLT69_08575"
     CDS             1080..1232
                     /locus_tag="PLT69_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14530.1"
                     /translation="MLAFVLVPGSALFVAYVRAHEAGIKRPVTVGIAAAALILAFLFF
                     SLGLVY"
     gene            1577..2647
                     /locus_tag="PLT69_08580"
     CDS             1577..2647
                     /locus_tag="PLT69_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ14531.1"
                     /translation="MDMKRDYYEDIELFQSNTVFFWTVVLMACLFTLPLYVESYNIYL
                     VNIILVHVIMAVGLNILVGYTGQISLGHAGFFAIGAYGTALISMHLHMPFVVSLIASG
                     LIAAFFGFILGLPALRLEGPYLAIATLGFGLTIMHVIGRWEVFGGRMGITVPPLDMGV
                     PGTPLSFVIEGDVKIYYLILVITILMVICARNLMKTRLGRAFVAIRDDDVAAEVMGVN
                     LTVYKTLSFAVSAFYAGIAGGLYGYVVSFFDPFTFNLILSIIFLVMVVVGGLGSILGS
                     VLGASLITYLQFDLLKNVEELPYLGDLLVLVSRKWLTVIGLANFSSIALGVIMICIVI
                     FEPLGMFGIWIRIKKYWKTWPF"
     gene            2666..>2958
                     /locus_tag="PLT69_08585"
     CDS             2666..>2958
                     /locus_tag="PLT69_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ14532.1"
                     /translation="MIEQLVVEGLAIGACYGLFGIAVAIIYKTSEVVNFAQGEMGMVA
                     TFVAFHILTVYHVPFWLAFVLTLLFAAALGALIQTCFLRPAKDPTVLGLIIIT"
BASE COUNT          506 a          945 c          909 g          598 t
ORIGIN      
        1 gtgatgatga agttcggcca gatgctgagc ttcatcatgg aggcgctgcc cgaggccgtg
       61 agacttcacc ggggaggcga cctggcgagc cgctacctgc ccgaggtcat ctgagcggcc
      121 ggcggggcgc tgcttccgcc gggcgtaacc cctcagcgca gatcgaggac ttctgaaccg
      181 gggcccgcct cgacgaggca tgccgtgtcc cttcggcaaa ggggcccatc ccggcccgca
      241 ccccctgtgg gatgaacggg cgaggagcgc tcgcccaagg ccgcggcgga gaccttcacg
      301 gccggtgcac gaggtcgtcc ccggctcctc gtgtgcacga cggacgaacg gtcaacatgg
      361 actgaacggg gcgtctcaga ccaccggcgg gaagtcgcgc cgcccttttc ggccaggggt
      421 gttaagtatt cccacggtca tgccgatagg tatatcagcc agacagagtc aacactctct
      481 tcgtaaccgc caggcgacga cagcttggct ttttttttac cgagacacag gcgttgtccc
      541 gttcaagaac gagcgcaccc tgacctttac ggatggccgc ggcaaggccg ggtttcaacc
      601 accgtgggcg agagaacccc tgtgacgcgc ggcagagacg cacacctcga gggaggggcc
      661 tccccctcgg gtcaccccgg aggcatggaa ttgccgtttc tgtgaccggg cacatccccg
      721 agggagggcc cagcccggct gcggcccacg aggaatgccg tgtttacgcc catgaccggc
      781 cgccttttca ggctggggat gccgccacct tcccggaagg gaagacacct cgcgcaccct
      841 tgctgccgca ccggtttctc tgtacggaga gacccctgcc ttgcagcagc cggtgtccct
      901 ggccaaaaag tgttgcactg gagatacgtg atgtattaat agggtttcca gatggaaatg
      961 agccctgcag cgagtgcaga gtggtttttc ttcgaggggg ttgaggcttg aagggcacac
     1021 cgagcccagg cctgcatcgg ggcctcctct ctcgaggcct cctgtcgagt tccttgctga
     1081 tgctcgcctt cgtcctggta cccggttcgg cgctcttcgt cgcgtacgtg agggctcatg
     1141 aggcagggat caagagaccc gtcacggtag ggatcgccgc cgcggccttg attctggcat
     1201 tcctgttctt ttccctgggt cttgtgtact gaggagcgag tgtggacgtc ccggtctctc
     1261 ttccccgcgt gcatgccatc cctgatccct tggcgtgttc gtgcgcacct aaagccggcc
     1321 cccctggttc acttgacagg gctccggcag ggattattat gaaaagcgtg gagatcacag
     1381 gcggcgtgaa cgacatcaat gaaaggtacc ccatcctcgg agagacgcct tgcccacccg
     1441 gcacgaccgc tgccgccgta gagcggcgat gccggactcg atgcgcaacg aagtaggatc
     1501 gtgaacgaag ggagcccgct gtccggccgg aaccggcctg accgggcggc cccgagccgt
     1561 gcaagagggt gatgccatgg acatgaagcg tgactactac gaggacatcg agctcttcca
     1621 gagcaacacc gtgtttttct ggaccgtggt gctcatggcc tgcctcttca ccctgcccct
     1681 gtacgtagag tcgtacaaca tctacctcgt gaacatcatc ctcgtgcacg tgatcatggc
     1741 agtggggctc aacatcctgg tggggtacac gggccagata tcgctcgggc acgccgggtt
     1801 cttcgccata ggcgcctacg ggaccgcgct catctcgatg cacctccaca tgccgttcgt
     1861 ggtctcgctc atcgcctcgg gcctgatcgc ggccttcttc gggttcatcc tgggtcttcc
     1921 cgcgctccga ctcgaggggc cttacctcgc catcgcgacg ctcggcttcg ggctcaccat
     1981 catgcacgtc atcgggcggt gggaggtctt cggtggcagg atgggcatca cggtgccgcc
     2041 gctggatatg ggggtgccgg gcacgccgct cagtttcgtc atcgagggcg acgtcaagat
     2101 ctactacctg atactcgtga taaccatctt gatggtcatc tgcgcccgca acctcatgaa
     2161 gacccgcctg ggcagggcat tcgtggcgat cagggacgac gacgtcgcgg cggaggtcat
     2221 gggcgtcaac ctcaccgtct acaagaccct ctctttcgcc gtcagcgcgt tctacgcggg
     2281 tatcgcgggc ggcctgtacg gctacgtggt gagcttcttc gaccccttca ccttcaacct
     2341 catcctctcc atcatcttcc tcgtcatggt ggtggtgggc gggctgggat ccatcctggg
     2401 ttcggtgctc ggcgcctcgc tcataacgta tctccagttc gacctcttga agaacgtgga
     2461 agaactcccc tacctggggg acctcctcgt cctcgtgtcg cgcaagtggc tgaccgtgat
     2521 cgggctcgcc aacttcagca gcatagcgct cggcgtgatc atgatctgca tcgtcatctt
     2581 cgaacccctg gggatgttcg gaatctggat caggataaag aagtactgga agacgtggcc
     2641 gttctgaggg aaaggcggaa acgagatgat cgaacaactc gtcgtcgagg ggctggcgat
     2701 aggcgcctgc tacgggctct tcgggatcgc cgtggccatc atctacaaga cctcggaggt
     2761 cgtgaacttc gcgcagggcg agatgggcat ggtggcgacc ttcgtggcct tccacatcct
     2821 caccgtctac cacgtcccct tctggctcgc gttcgtcctc accctgctct tcgccgccgc
     2881 cctgggagcg ctcatccaga cgtgcttcct gaggccggcc aaggacccca cggtcctcgg
     2941 tctcatcatc atcacctt
//
LOCUS       DAOSCR010000342         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21657_length_2950_cov_3.134715, whole genome shotgun sequence.
ACCESSION   DAOSCR010000342 DAOSCR010000000
VERSION     DAOSCR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21657_length_2950_cov_3.134715"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /gene="ispH"
                     /locus_tag="PLT69_08590"
     CDS             <1..1592
                     /gene="ispH"
                     /locus_tag="PLT69_08590"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOJ14533.1"
                     /translation="GVRRAIDITFKVKQRNKDRRIFTLGQIIHNPQVIDALTKRGIGI
                     VHSIEDPQLRPGDIAIVRAHGISPDKKKALTERGVEVIDAACPMVLKLHSVIKKACRT
                     ADLIAIVGDKDHPEMDAHMGVAKGKGTIVEKLEDAKRLPRVKRMAVVAQTTFDVALYR
                     EIVEVLKEKAEILDVSDTICRSTVDRQTEVRELARDHDTFIVIGGRNSANTNRLAEIA
                     AKENRHVIKVESPEQLRDIDRSRMKRVAVIAGASTPHWVIEECIEELRSAHPASTIET
                     TVLDTLAESPLLPSLGALGVAMAAFVFSGQPYRWDVLTAVFFLAFSSTAHTKRWRQWP
                     TWAISTGIALSLSLVVSGLPGGLMFVAISLLRPLVDVMDTAEYIKSVIGGLTFILISI
                     VVPLALTQAAPTPGTGLLAAYTLTHYLGAEILIGLKNMEKDAIMGTMSLARYISEDRA
                     VTLMEYTIMGLALMLFLSFPLKVAPALAYGLLPSLFFLAKGIDFHHDQRIFDNRMYTI
                     YVMALWAIIPAMGLLWLATMM"
     gene            1870..2472
                     /locus_tag="PLT69_08595"
     CDS             1870..2472
                     /locus_tag="PLT69_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOJ14534.1"
                     /translation="MPALPTATQLSAHAQGAPSRASRPGRVAFAPAPPYVNTIPPRRF
                     CMGEKDLGYYIDLAIQREEEAYAFYVDLAAKVADRSAKDALELLAGEERKHKAFLVTY
                     REGGYEEGALRMNHPIDYKIAQHMEKPDIDKDMKSKDVFLVAAHRELNSYNFYNELAG
                     LHPEGELRQLFLKMASEELRHKEKVEYLYSNVAFAQTDGG"
BASE COUNT          587 a          984 c          922 g          457 t
ORIGIN      
        1 tcggcgtgcg cagggccata gacatcacct tcaaggtgaa gcagcgcaac aaggacagga
       61 ggatcttcac cctggggcag atcatccaca acccgcaggt catcgatgcg ctcaccaaga
      121 ggggcatcgg catcgtccac agcatagagg accctcagct caggcccgga gacatagcca
      181 tcgtgagggc gcacggcatt tcgccggaca agaagaaggc cctcaccgag cgcggcgtgg
      241 aggtgatcga cgcggcatgc cccatggtgc tcaagctgca cagcgtgatc aagaaggcgt
      301 gcaggaccgc cgacctcatc gccatcgtcg gcgacaagga ccacccggag atggacgccc
      361 acatgggggt ggcgaagggc aagggaacga tcgtcgagaa actcgaggac gcgaagaggc
      421 tgccccgggt gaagaggatg gcggtggtcg cccagaccac cttcgacgtg gccctgtaca
      481 gggaaatcgt ggaggtgctt aaggaaaagg ccgagatcct cgacgtgtcc gacaccatat
      541 gcaggtccac cgtcgaccgc cagaccgagg tgagggaact cgccagggac cacgacacgt
      601 tcatcgtgat cggcggccgg aattccgcga acacgaacag gctcgcggag atcgcggcga
      661 aggagaaccg ccacgtgatc aaggtggagt cgccggagca gctcagggac attgacaggt
      721 cgcgcatgaa gcgcgtggcc gtcatagccg gggcgtccac gccccactgg gtgatcgagg
      781 agtgcatcga ggagctcagg agcgcccacc cagcctccac catcgagacg accgtcctgg
      841 acacccttgc cgaatcgcct ctcctcccct ccctcggggc cctgggggtg gccatggcgg
      901 cgttcgtgtt cagcgggcag ccctaccgtt gggacgtcct cacggcggtc ttcttcctcg
      961 ccttctcctc gaccgcgcac acgaagaggt ggagacaatg gcccacgtgg gccatctcga
     1021 cgggcatcgc cctgtcgctg agcctcgtcg tgagcgggct ccccggcggc ctcatgttcg
     1081 tggccatctc cctgctcagg cccctggtgg atgtcatgga caccgcggaa tacatcaaga
     1141 gcgtcatcgg cggcctcacc ttcatcctca tctccatcgt ggtacccctc gcactcacgc
     1201 aggcggcacc cacccccggg acaggactcc tcgccgcgta caccttgacc cactacctcg
     1261 gcgccgagat cctgatcggg ctcaagaaca tggagaagga cgccatcatg ggaacgatga
     1321 gcctggctag gtacatctcc gaggatcgcg ccgtgacgct catggagtac accatcatgg
     1381 ggctcgccct catgctcttc ttgagcttcc ccctcaaggt ggcccccgcc ctggcctacg
     1441 ggctgctgcc gtccttgttc ttcctggcca agggcatcga cttccaccat gaccagagga
     1501 tcttcgacaa ccgcatgtac acgatctacg tgatggcgct ctgggcgatc atccccgcca
     1561 tgggcctcct ttggctggcg accatgatgt gaacacccgc cctggcccct ggaccccctc
     1621 gcggacgaat ggaaaggctc cctgacgggc cggtctgtcg cgggccggac ccaggcgcgc
     1681 ggcgcccgcc cctgcgggcg gtcgacgggc ggccccccac ccccgggagc tcggatgctc
     1741 gaggatcaca gggcggcaaa cggtgcaagc aggcgaccgt gctaagcggg caggcctccc
     1801 gcagaacctg tggcggtgca tgtcgcggag ctgagcccac acccgatcaa cgcgatcttg
     1861 ccgtccctcg tgcctgcact gccgacggcg acgcagcttt ctgcccacgc gcagggtgcg
     1921 ccctcgaggg cgagcagacc cggccgtgtt gcctttgccc ctgcaccgcc ttatgttaat
     1981 accattccac cgaggaggtt ttgcatggga gagaaggatc tcggttatta catcgacctg
     2041 gccatccagc gcgaggagga ggcctacgcg ttctacgtgg acctcgccgc gaaggtcgcc
     2101 gaccgctcgg cgaaggacgc gctcgagctc ctcgccggag aggagcgcaa gcacaaggcc
     2161 ttcctcgtga cgtaccggga gggggggtac gaggagggcg cgcttcgcat gaaccacccc
     2221 atcgactaca agatcgccca gcacatggag aaacccgata tcgacaagga catgaagtcc
     2281 aaggacgtct tcctcgtcgc ggcgcaccgc gagctcaact cgtacaactt ctacaacgaa
     2341 ctggccggcc tgcaccccga aggagagctc aggcagctct tcctcaagat ggcgagcgag
     2401 gaactcaggc acaaggagaa ggtcgagtac ctctactcga acgtggcctt cgcccagacc
     2461 gacgggggct gacaccaggc cggagccgcc gcgccttggg gagacgacgc gcaccagggc
     2521 ggagagcccc cgctcaagga gcaggtccga ccccggtcgt cccgtcccgc ccgggcgtca
     2581 tgggacgatc tcacccggta gctatttgtg aggtgccgcc caaccccgcg tactttcaaa
     2641 gggcattctt caagttgcgt gcaggcgcct ccgaaaagcc accttgacgg cgcgtcttcc
     2701 cggctccatt atgaaggcca aggtcacggg caactcccct gaacccgcct tcagagaacc
     2761 cggatcgccg cccgagccgc aagccggcaa ggggatttcc tcgaaagggg gtatcgaatg
     2821 tgaaggagaa ggaaaaggat agggagaaga actacttcgc cgcgctctac gaggtcgtgc
     2881 gcatggtcca cggctccctc gacgtgagca cggtgctcca ggagatcgtc aaggccgtga
     2941 cggaagccat
//
LOCUS       DAOSCR010000343         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21874_length_2928_cov_3.969370, whole genome shotgun sequence.
ACCESSION   DAOSCR010000343 DAOSCR010000000
VERSION     DAOSCR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21874_length_2928_cov_3.969370"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLT69_08600"
     CDS             <1..946
                     /locus_tag="PLT69_08600"
                     /inference="COORDINATES: protein motif:HMM:NF028299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HOJ14535.1"
                     /translation="TDSGPAEGFSWNRWQESRQVGYEKDTKNQWGDTDENKMRLRQEQ
                     LAEGRIAAETFDEAVARSSREFYIRLSDSIDATLEAFGRLDRVVDERFGNEAPKLSDL
                     KKAIEDCSRLVAQFLKDKGGKDDPGRREARQGEDAAAGQRQQPVQGGTGRKAQAVRAQ
                     AQAGSDGMGVQPAAPFQVFSDSASQEEALWQAARQTLEAGGINAALAQLLAASASAPS
                     PRQKNRYRLLMAKLALTANRPDIARPIVEELYGLISELNLERWESPLWIAEVIEAYYL
                     CLTGEGASEEDRARADNELFPKLCSLDITKALAYKKGG"
     gene            950..1471
                     /gene="tssB"
                     /locus_tag="PLT69_08605"
     CDS             950..1471
                     /gene="tssB"
                     /locus_tag="PLT69_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019333264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HOJ14536.1"
                     /translation="MAKKESLQHTLDRVRAPRVQITYDVEIGDSIEMKEIPFVVGVLG
                     DFSGKPDEPLPRLKERKFIEIDRDNFNKVLEGMKPRVSFRVDNKLTGDGSQIAVDLRF
                     RSMDDFHPERVAQQVQPLRKLVEARRRLADLLNRLDGNDRLDELLQEVISSTDSLQKL
                     GKEAGVPAEEKKE"
     gene            1484..>2928
                     /gene="tssC"
                     /locus_tag="PLT69_08610"
     CDS             1484..>2928
                     /gene="tssC"
                     /locus_tag="PLT69_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HOJ14537.1"
                     /translation="MAEKDPKKEQAPAAQEVVEEKSLLDRIIEQGRMARDEAQREHAR
                     DLIAEFVSQVMEGAMAVSKDTEAMITARIAQIDRLISDQLNEIMHHPDFQRLESSWRG
                     LFYLVNQSETGEKLKIRVMNATKAELLKDMEKATEFDQSTLFKKVYEEEFGMFGGASY
                     GALVGDYEFGNTPQDLSLLEKISQVAAAAHAPFISAASPQMFNLDSFTELGQPRDLAK
                     IFQGVEYAKWRSFRETEDSRYVALTLPHVLMRLPYGSANVPVEAFDFEEDVTGAEHDK
                     YLWGNAAYALASRLTDAFAKYHWTAAIRGVEGGGLVQGLPVHTFKTDEGDVALKCPTE
                     IAITDRREKELADLGFIPLVHCKGTDYAAFFSTQTVNKPKAYDTDAANANARLSAQLQ
                     YILAVSRFAHYMKSIMRDKIGSFMTRKDAEDFLNRWISKYVLLDDSAGQEIKASYPLR
                     EARVEVVEIPGKPGCYRAVAFLKPHYQLDELS"
BASE COUNT          617 a          888 c         1004 g          419 t
ORIGIN      
        1 gacggactcg ggcccggcgg agggcttttc ctggaacagg tggcaggagt ccaggcaggt
       61 gggttacgag aaggacacca agaaccagtg gggggacacc gacgagaaca agatgcgcct
      121 tcgccaggaa cagctggccg agggcaggat cgccgccgag accttcgacg aggcggtggc
      181 gcgctcgtcc agggagttct acatccgcct gtcggacagc atcgacgcga cgctcgaggc
      241 gttcgggagg ctcgacaggg tcgtggacga gaggttcggg aacgaggccc ccaagctctc
      301 cgacctgaag aaggcgatcg aggactgctc gaggctcgtg gcccagttcc tcaaggacaa
      361 ggggggaaag gacgaccctg ggcggcggga ggcccggcag ggggaggatg ccgctgcagg
      421 gcagcggcaa cagccggtgc agggcggcac gggcagaaaa gcgcaggccg tgcgtgcaca
      481 ggcccaggcc ggctctgacg gcatgggcgt ccagcccgcc gcgcccttcc aggtcttctc
      541 ggactcggcg tcacaggagg aggcgctctg gcaggcggcc cgccagacgc tggaggccgg
      601 cggtatcaac gcggccttgg cccagctcct cgccgcatcg gcgagcgcgc catccccgag
      661 gcagaaaaac cgataccggc tcctgatggc caagctcgcc ctcacggcga accggccgga
      721 catcgcgagg cccatcgtgg aggaactcta cggactgatc tcggagctga atctcgagcg
      781 gtgggagtcc cctttgtgga tcgcggaagt catcgaggcg tactacctct gcctcacggg
      841 agagggcgcg agcgaggagg acagggcgag agcggacaac gagctcttcc ccaagctgtg
      901 ttccttggac atcaccaagg ctctggcgta taagaaagga gggtgaccca tggcaaagaa
      961 ggagagtctg cagcataccc tcgacagggt gagggcgccc agggtgcaga tcacctacga
     1021 cgtggagatc ggtgactcca tagagatgaa ggagatcccg ttcgtcgtcg gcgtcctggg
     1081 ggacttctcg ggcaagcccg atgagcctct gccgaggctg aaggagcgga agttcatcga
     1141 gatcgaccgc gacaacttca acaaggtgct cgaggggatg aaaccgaggg tgtctttcag
     1201 ggtggataac aagctgaccg gcgacggttc gcagatagcc gtggacctca ggttcaggtc
     1261 catggacgat ttccaccccg agcgggtggc gcaacaggta cagccgctcc gcaagctcgt
     1321 cgaggccagg aggaggctcg ccgacctcct gaacaggctc gatggcaacg acaggctgga
     1381 cgaactgctc caggaggtca tctcgagcac ggactccctg cagaagctgg ggaaggaggc
     1441 gggggtcccg gcagaggaaa agaaagagtg aggggggatg cccatggctg agaaggaccc
     1501 gaagaaggaa caagctccgg ccgctcaaga ggtcgtcgag gagaagagcc ttctcgaccg
     1561 gatcatagag cagggccgca tggcgcgcga cgaggcccag cgggaacacg ccagggacct
     1621 catcgcggag ttcgtgagcc aggtcatgga aggggccatg gccgtgtcga aggacaccga
     1681 ggccatgatc acggcccgga tcgcccagat cgacaggctc atatccgacc agttgaacga
     1741 gatcatgcac cacccggact tccagaggct cgagtcttcc tggcgcggcc tgttctacct
     1801 ggtgaaccag agcgagacgg gcgagaagct caagatcagg gtcatgaacg cgacgaaggc
     1861 cgaactcctc aaggacatgg agaaggcgac cgagttcgac cagtcgaccc tgttcaagaa
     1921 ggtctacgag gaggagttcg gcatgttcgg cggggcctcc tacggcgccc tcgtcggcga
     1981 ttacgagttc gggaacaccc cccaggacct ctcgctgctc gagaagatct cccaggtggc
     2041 ggccgcggcg cacgccccgt tcatctcggc cgcgtccccc cagatgttca acctcgacag
     2101 cttcaccgag ctgggccagc ccagggacct cgcgaagatc ttccaggggg tggagtacgc
     2161 caaatggagg tcgttccgcg aaaccgagga ttcccggtac gtggccctca ccctgcccca
     2221 cgtgctcatg aggctgccct acggttcggc caacgtcccg gtcgaggcct tcgacttcga
     2281 ggaagacgtc acaggggcgg agcacgacaa gtatctgtgg ggcaacgccg cctacgcact
     2341 ggcctctcgg ctcacggacg ccttcgccaa gtaccactgg acggcggcga tcaggggggt
     2401 cgagggaggg ggcctcgtgc agggcttgcc cgtgcacacc ttcaagaccg acgaggggga
     2461 cgttgcgctc aagtgcccga ccgagatcgc gatcacggac cgcagggaaa aggagctcgc
     2521 cgacctgggc ttcatcccgc tcgttcattg caaggggacg gactacgccg cgttcttcag
     2581 cacccagacc gtcaacaagc cgaaggccta cgacacggac gcggccaacg cgaacgcgcg
     2641 cctgtcggcg cagctgcagt acatactcgc ggtgtccagg ttcgcccact acatgaagtc
     2701 catcatgcgc gacaagatcg gcagcttcat gacccgcaag gacgccgagg acttcctgaa
     2761 caggtggatc agcaagtacg tgctcttgga cgacagcgcg gggcaggaga tcaaggccag
     2821 ctatcccctc agggaggcca gggtcgaggt cgtggagata ccggggaagc ccggctgtta
     2881 cagggccgtg gccttcctca agccgcacta ccagctcgac gagctctc
//
LOCUS       DAOSCR010000344         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21875_length_2928_cov_3.077271, whole genome shotgun sequence.
ACCESSION   DAOSCR010000344 DAOSCR010000000
VERSION     DAOSCR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21875_length_2928_cov_3.077271"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLT69_08615"
     CDS             complement(<1..470)
                     /locus_tag="PLT69_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ14538.1"
                     /translation="MRAHTILPMLALFAAMVLWAGSFIALKIAFFAYDPMLVIFLRMA
                     IASLGFVPLASRMGPNVPRPGDVPLILFMLACEPCLYFLFEAAALVNTSASQAGMITA
                     MLPLMVAVGAGFFLGEKVGARTYAGFGLAIAGATLLSLGAGSGRSSPNPALGNF"
     gene            1210..1392
                     /locus_tag="PLT69_08620"
     CDS             1210..1392
                     /locus_tag="PLT69_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14539.1"
                     /translation="MQMTFCPLCKGRLAVKTVDGRQRLCCSSRTCSYVFWDNPVPVVA
                     ALVEDGAGVVLVRNKA"
     gene            1402..1695
                     /locus_tag="PLT69_08625"
     CDS             1402..1695
                     /locus_tag="PLT69_08625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ14540.1"
                     /translation="MFGLVTGFLERGETPEDAVLREVEEELGLKGELIGLIGHYSFFE
                     MNQLILAFHVRVEGEPRLGGELAEYRRVSPEKLRPWPFGTGYAVRDWLEMRRR"
     gene            1853..2449
                     /locus_tag="PLT69_08630"
     CDS             1853..2449
                     /locus_tag="PLT69_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ14541.1"
                     /translation="MTLLVFLLGVCAPPAEAARNPRVILKTTKGDITIELFEDKAPKT
                     VRNFLEYVDKGFYDGTIFHRVIRGFMIQGGQMRPDMGEKPAGAPIQNEADNGLKNLRG
                     TVAMARTPHPHSACVQFFINTKDNPFLDFRSPTFEGYGYCVFGRVIKGMDVVDAIERV
                     PTTTRGFHQDVPVESVIIEKATRVAPADPKADTGKPAQ"
     gene            2510..>2928
                     /locus_tag="PLT69_08635"
     CDS             2510..>2928
                     /locus_tag="PLT69_08635"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ14542.1"
                     /translation="MLAWAFYDWGNSAFATTVMAGFFPIVFKEYFSAGVEAAQSTARL
                     GFANSAAGLAVAVAAPFLGAVADRASSKKLFLACFAATGVLSTVGLSLVGMGSWVLAC
                     TLYGLGLAGFLGGNVFYDSLLKRICSEGETDRVSSLG"
BASE COUNT          543 a          930 c          995 g          460 t
ORIGIN      
        1 aagaagttgc cgagtgcggg gttcggggac gaccggccgg agccggcccc caggctgagc
       61 agagtcgcgc ccgcaatggc gagaccgaat cccgcgtacg tccttgcgcc caccttctcg
      121 ccgaggaaga agcctgcgcc cacggccacc atgagcggga gcatggcggt gatcatgccc
      181 gcctgggagg ccgaggtgtt cacgagggcg gccgcctcga agaggaaata caggcacggc
      241 tcgcaggcga gcatgaacag gatgagcggt acatcgccgg gcctggggac gttcggcccc
      301 atcctcgatg caaggggcac gaacccgaga gatgcgatgg ccatcctcag gaagatcacg
      361 agcatggggt cgtaggcgaa gaacgctatc ttgagcgcga tgaaagatcc cgcccagagt
      421 accatggcgg cgaacagggc gagcatcggc aagatggtgt gagcgcgcac tgcgtcttgg
      481 cctcccctcc cgtgatcatc ggctggtgtc ccctcgggat agcacatgag accagcccgg
      541 gagaaccccc attcacacgc aggcgcactg ggggtgcatc cttgcgtgat cagggggaaa
      601 accccttgcc cggttgtcga gggaaacccg gggagccagg ggccgcgcca gggccctgaa
      661 accggccacg ccggcatcgc ggaacccagg caccccatcg cttcagcgcg cacaccggga
      721 aaggccttcc acgcttggat gcacacccac acaaggacca tgcaccgcgc gtcccgcacg
      781 ggctgagctc caggcggcac cgctaaaccg ccgcgctcac ggcagcccct gcgcaggtac
      841 cgacctcgtg gccgcacccg gcggcacatc gtccacatcg aggagcctgg ttctcaccgc
      901 cgcatcccta gggcccgccc tgggacacct tgggccggca gagatgtcgg ggcgcatcga
      961 ggggcttctc ggcccaagcg tgccctgtct ccatgtgctt cgtcttcttg ccggtcttgc
     1021 ccgcaagagg cggcgaacac gacccgcccg cagccggcag cggctggcgt atctcttgga
     1081 gcagaggctg tgcggcctcc gtgggatcca caccgggcaa gacgccgttg cccaccggag
     1141 aagacagggc gtatagagac ggttcatgga ccccctgatg aaagcgcacg ccgcccggga
     1201 aggcacgcca tgcagatgac cttctgcccc ctctgcaaag gccgcctcgc cgtgaagacc
     1261 gtggacggga gacaacgcct gtgctgttcg agccggacat gttcttacgt gttctgggac
     1321 aacccggtac cggtggtggc cgccttggtg gaggacggcg ccggggtcgt gctcgtgcgc
     1381 aacaaggcct gacccgagaa gatgttcggg ctcgtgaccg ggtttctgga aaggggggag
     1441 accccagagg acgcggtgct tcgcgaggtc gaggaggagc tggggctgaa gggcgaactc
     1501 ataggcctca taggtcacta cagcttcttc gagatgaacc agctcatcct cgcctttcat
     1561 gtccgcgtcg agggtgaacc ccgcctggga ggcgagctcg ccgagtacag gcgtgtcagc
     1621 cccgagaaac tcaggccgtg gcccttcggt accgggtacg cggtgaggga ttggctggag
     1681 atgagaaggc gatgaggccc gtggtgggtg cgtcttctgg cctgtggaga cgtacaagac
     1741 cttcacggcc cttggaggtg agacgatgag acctggaacg aaacccgacc cgattttgcg
     1801 tgcaggtaga gaggcacacg ctgcagcccg cacagggaaa ggagtctacg tcatgacgtt
     1861 actggttttc ctgctgggtg tgtgcgcacc cccggccgag gcggcccgca acccgagggt
     1921 catcctgaag acgacgaagg gtgacatcac catcgagctc ttcgaggaca aggcgcccaa
     1981 gacggtgagg aatttcctcg agtacgtgga caaggggttc tacgacggca ccatcttcca
     2041 ccgggtcatc agggggttca tgatccaggg cggccagatg aggccggaca tgggcgagaa
     2101 gcccgccggc gcgcccatac agaacgaggc ggacaacggg ctgaagaacc ttcggggcac
     2161 cgtcgccatg gcgaggaccc cgcaccccca ctcggcatgc gtccagttct tcataaacac
     2221 gaaggacaac ccgttcctcg actttcgctc ccccaccttc gaggggtacg gctactgcgt
     2281 gttcggccgg gtgatcaagg gcatggacgt ggtggacgcg atcgaacggg tgcccacgac
     2341 gacgaggggc tttcaccagg acgtgcctgt ggaaagcgtg atcatcgaga aggcgacgag
     2401 ggtcgccccc gccgacccga aggctgacac gggcaagccc gcccagtagg acatgcagcc
     2461 cggcccgatg cagccgcatc gcgagacgga ggggaagccc cggcgacagg tgcttgcgtg
     2521 ggcgttctac gactggggga actccgcttt cgccaccacg gtcatggccg ggttctttcc
     2581 catcgtcttc aaggaatact tcagcgccgg agtggaggcg gcccagagca cggcaaggct
     2641 ggggttcgcg aactcagcgg cgggtctcgc cgtcgccgtt gcagcgccct ttctgggcgc
     2701 cgtcgcggac agggcctcgt cgaagaagct cttcctggca tgcttcgccg ccacgggcgt
     2761 cctctcgacc gtggggctgt cgctcgtcgg catggggtcc tgggtcctcg catgcaccct
     2821 ctacgggctg gggctcgcgg ggtttctcgg cgggaacgtg ttctacgact ctctcctgaa
     2881 gcgcatctgc tcagagggcg agacggaccg cgtctcgtcc ttgggcta
//
LOCUS       DAOSCR010000345         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21928_length_2924_cov_3.323109, whole genome shotgun sequence.
ACCESSION   DAOSCR010000345 DAOSCR010000000
VERSION     DAOSCR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21928_length_2924_cov_3.323109"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..1701
                     /locus_tag="PLT69_08640"
     CDS             355..1701
                     /locus_tag="PLT69_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14543.1"
                     /translation="MKKALLAAFIVLFATAAWAEDVKFSISGDFHARGSYWKNFSQGA
                     PAADYESESYMDYDGDINLYPKLSVGNATLNFKIAIRDETLGSIANDVSKSVNTDWTS
                     MAGQTYTTQKDDNINIERAWLTYKFGENTTLDVGLMDGQAWATTFGDYCMGRYRVKLT
                     QKTPFGVIEGGLEKNVELGTSALKDSEKDDYDSYFLSAVTKAGDIYIKPLIFYVDNSA
                     ALSSSAPSIGETSDGVKVLYLALGFNGKLGALDFESELGYKKADWEDFEQLADYAAVN
                     PALAAYANAKNSNVWGAYFNVWKSMDALTPGITLAYGSWDDEAGARGTGWGFDFRDDF
                     CSNLILGDWIAWSDITGNEEDLVAATLIKPYVTDVKLADKLSASASFGYIMSNQKDNA
                     YEDATAWEIDLGLAYKLSDNVTYSVDAGYADISYDVEGVEDPDEIMLLSHKIKFTF"
     gene            complement(1819..>2924)
                     /gene="rpoD"
                     /locus_tag="PLT69_08645"
     CDS             complement(1819..>2924)
                     /gene="rpoD"
                     /locus_tag="PLT69_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOJ14544.1"
                     /translation="VSRDIVRLIHDIHFMPAQIELFGNRLKELKRQIEAKEEALSLYE
                     QRFARDLDEIRAIYEAACTDPVDAEACAFLERHSLGVQELSDIVQDIARVSREIKSLE
                     VEGGLTSHGIKKAVRILEEAEARTNAAKTELIQANLRLVVSIAKKYVNRGLQFLDLIQ
                     EGNIGLMKAVEKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLI
                     RTSRALVQELGREPTPEEIAAKMDYPEHKVRKILKIAKEPISLETPIGEEEDSHLGDF
                     IEDKKVPSPSDAILAKNLSEQSRRVLATLTPREEKVLRMRFGIGEKSDHTLEEVGKDF
                     TVTRERIRQIEAKALRKLRHPVRSKKLRSFIDS"
BASE COUNT          570 a          900 c          895 g          559 t
ORIGIN      
        1 cgtgatcggg ggtctcaaaa gaaagatcgc gcccggtgcc cggacttcaa gcaccccgat
       61 cccgaggaaa aacggccaag aaaacgggtt gtggtccaag gggctggtgc gtcgaaggcg
      121 cacccgagac tgtcttcttt ttacaacgaa gaacggcaag catgtggtgt gtttcccaca
      181 cggccgggac tcagattatg cgcaattact cgttattacg tcatttttct atttggcatg
      241 attgttgtaa aaggatctgt gtacgagaga agacataagg tatgtgcaca gggggcacct
      301 gaggacggtt gaaagacaag atctcgagaa aacaaacgaa aaggaggaga taggatgaag
      361 aaggcacttt tggcagcgtt catcgtactg tttgccacgg ctgcgtgggc ggaagatgtg
      421 aagttcagca tctccggtga cttccacgcc cgcggcagct actggaagaa cttcagccag
      481 ggcgctccgg ctgcggacta cgagagcgag tcctacatgg actacgacgg ggacatcaac
      541 ctctacccca agctctcggt cggcaacgcg acgctcaact tcaagatagc catccgtgac
      601 gagacgttgg gatccatcgc gaacgacgtc tccaagtccg tgaacaccga ctggacttcc
      661 atggcgggcc agacctacac gacccagaag gacgacaaca tcaacatcga gcgcgcctgg
      721 ctcacctaca agttcggcga gaacaccacg ctggatgtcg gcctcatgga cggccaggcc
      781 tgggcgacga ctttcggtga ctactgcatg ggccgctacc gcgtgaagct cacccagaag
      841 acccccttcg gcgtgatcga gggcggtctc gagaagaacg tggagctcgg cacctcggcc
      901 ctgaaggact ccgagaagga cgactatgac tcctacttcc tctccgccgt caccaaggcg
      961 ggcgacatct acatcaagcc cctgatcttc tacgtcgaca acagcgcggc gctctccagc
     1021 tccgccccga gcatcggcga gacgagcgac ggcgtcaagg tgctctacct cgccctcggc
     1081 ttcaacggca agctcggtgc gctggacttc gagagcgagc tcggctacaa gaaggccgac
     1141 tgggaagact tcgagcagct cgccgactac gccgcggtca acccggctct cgctgcatac
     1201 gccaacgcga agaacagcaa cgtctggggc gcgtacttca acgtctggaa gtccatggac
     1261 gccctcaccc ccggcatcac gctggcctac ggcagctggg atgacgaggc cggcgccagg
     1321 ggcaccggct ggggcttcga cttccgtgac gacttctgct ccaacctgat cctcggcgac
     1381 tggatcgcct ggagcgacat caccggcaac gaagaagacc tcgtggccgc aaccctcatc
     1441 aagccctacg tcaccgacgt gaagctggcc gacaagctct cggcgagcgc ctccttcggc
     1501 tacatcatgt ccaaccagaa ggacaacgcg tacgaggacg cgaccgcctg ggaaatcgac
     1561 ctcgggcttg cgtacaagct cagcgacaac gtgacctact cggtcgacgc cggctatgcc
     1621 gacatctcgt acgatgtcga gggcgtggaa gacccggacg agatcatgct gctctcccac
     1681 aagatcaagt tcaccttcta ggcacgatca tcccaagggg aaacacgaag ggcccgccat
     1741 ctggcgggcc cttttttcat cctttgtaca ggccgtcgta caggccgggg gatcgcggcc
     1801 tggagcggcc acgtgcggct acgagtcgat gaacgagcgg agcttcttgg agcggaccgg
     1861 gtgcctgagc ttgcgcaggg ccttggcctc gatctggcgg atgcgctcgc gcgtgaccgt
     1921 gaagtccttg cccacctcct cgagggtgtg gtcggacttc tcgccgatgc cgaagcgcat
     1981 gcgcagcacc ttctcctcgc gcggggtcag cgtggccagg accctgcgcg actgctccga
     2041 gaggttcttg gccaggatgg cgtccgacgg cgaaggcacc ttcttgtcct cgatgaagtc
     2101 gcccaggtgg ctgtcctcct cctcgcctat cggtgtctcg agcgagatgg gctccttggc
     2161 gatcttgagg atcttgcgca ccttgtgctc ggggtagtcc atcttggcgg ctatctcctc
     2221 gggcgtgggc tccctgccga gctcctggac gagcgcccgg gaggtcctga tgagcttgtt
     2281 gatggtctct atcatgtgca ccgggatgcg gatggtcctg gcctggtcgg cgatagcccg
     2341 ggtaatggcc tgcctgatcc accaggtggc gtaggtggag aacttgtagc ccctcctgta
     2401 ctcgaacttc tccaccgcct tcatgaggcc gatgttgccc tcctggatca ggtcgaggaa
     2461 ctgcaggccc cggttcacgt acttcttggc aatgctcacc acgagccgta agtttgcctg
     2521 gatgagctcg gtcttggccg cgttggtccg ggcctcggcc tcctccagga tcctgaccgc
     2581 cttcttgatt ccgtgggagg tgagtccgcc ctcgacctca agggacttga tctcgcggct
     2641 gacccgcgcg atgtcctgga cgatatccga gagttcctgc acgcccaggg agtgcctctc
     2701 gagaaaggcg cacgcctcgg cgtcgacggg gtccgtgcag gcggcctcgt agatggccct
     2761 gatctcgtcg aggtccctgg caaagcgctg ttcgtagaga gacagggcct cttcctttgc
     2821 ctcgatctgg cgcttgagct ccttgagccg gtttccgaag agctcgatct gcgcgggcat
     2881 gaaatggatg tcgtggatga ggcggacgat gtcgcggctc acgg
//
LOCUS       DAOSCR010000346         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_21978_length_2919_cov_3.445880, whole genome shotgun sequence.
ACCESSION   DAOSCR010000346 DAOSCR010000000
VERSION     DAOSCR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21978_length_2919_cov_3.445880"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1951)
                     /locus_tag="PLT69_08650"
     CDS             complement(119..1951)
                     /locus_tag="PLT69_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOJ14545.1"
                     /translation="MAWKPPARLTVSEWAEAERVLDTRTSSRPGPWRNARTPYLAGMM
                     DVFTLPYVNEITYMTGTQVGKTEAILNQIGWVVSQDPGSAMVVYPTEDIARGVSKLRI
                     QPLLETSPTVAERHIPSESTTLEIHFWGGYICLSGANSPASLSSKPIRYLWLDEVDKY
                     PPYLGEEADPISLARERTKTYTNRKVVQCSTPTVEKGAIYQSYLSADVRYAYHVPCPY
                     CGYMQPFTMSGIKFPEQIREDMKNAKGDPEKLREVAQRARASSWYECSRCGEAITDSQ
                     KMSMLLKGLWVPDKDVERPRHVAFHLNSIYAPALTFGDVAAEFIQAKDFPDRLRNFIN
                     SWLGEPWRENNIEIAVSEVMRHETQVPRGQVPRDAWFLTGGVDVQKDYMVWEVRAWGR
                     GATSWLVDCGRAETWDSIASIMDRGIWRTDDSRVFHVRLCLVDAGFRTDEVYAFCSKR
                     PDLYLPCKGSSRPLNGKLYTVTNIDRQGAAPMKLWIHDSGYWKGWVHSRIAREPGELG
                     AWRVFARCPKDYAEQIVAERKVMALNRRTGGYEEVWEKVTSHAANHFLDTAVLNALAA
                     ELLQIRYMDATEPPKETKAAETPPPAAKKPERYRPVVSDWVMGR"
     gene            complement(1914..2531)
                     /locus_tag="PLT69_08655"
     CDS             complement(1914..2531)
                     /locus_tag="PLT69_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14546.1"
                     /translation="MQGKTENEAPEVIKTADFLVTIHVLSEIMGISIPTLRKYVNNFG
                     APQHSHGLYRLKDFVQWYLKSYGIASESGEQDDGAESDEGSLKRRQTKAETRYREAKA
                     AQAELQLKILRGEYLPREEVEAEWASRVSAVRVGLLNFAKALPLDLVGKDAQGIEVVL
                     RREAESLLNEYSRSGQYTPEVEPAERGVAPEGEDGVEAAGKAHGE"
BASE COUNT          515 a          923 c          809 g          672 t
ORIGIN      
        1 gaccttcagg tctccccgtg tcatgcgccg cccgccgatg tagtattcct gtccgccctg
       61 gtatatggcc gcgatcgcat tgttaaccag ctccagctcc tccgcgtagg tcatcgtgtc
      121 acctccccat cacccagtcg ctgacaactg gccggtagcg ttcaggtttt ttcgcagccg
      181 gtggcggcgt ctcggccgct ttcgtctctt tcggcggctc cgtggcatcc atgtagcgga
      241 tctggaggag ctccgcagcc agcgcgttca ggaccgccgt gtcgaggaag tgattcgccg
      301 cgtgtgacgt gaccttctcc catacttcct catagccgcc ggttcggcga ttcagcgcca
      361 tgaccttcct ttccgccacg atctgctctg cgtagtcttt tgggcacctg gcaaacaccc
      421 gccacgcccc cagctcgccg ggctcccgtg caatccgcga atggacccag cctttccagt
      481 agcccgaatc gtgtatccag agcttcatcg gggcagcccc ctgccggtcg atgtttgtca
      541 ccgtgtatag ctttccgttg agcgggcggc ttgaaccttt gcacggcaga tacaggtcag
      601 ggcgtttcga gcagaacgca tatacctcgt ctgtcctgaa ccctgcatcc acgaggcaga
      661 gcctcacgtg gaagacccgg ctgtcgtcgg ttcgccagat tccgcggtcc atgatcgacg
      721 cgatgctatc ccatgtctcg gcccggccgc agtccacgag ccacgacgtg gccccgcgcc
      781 cccatgccct gacctcccag accatgtaat ccttctgcac gtcgaccccg cccgtgagga
      841 accacgcatc ccgcggcacc tgcccacgcg gtacctgcgt ctcgtgacgc atgacctcgc
      901 tgacggctat ctcgatgttg ttctcgcgcc acggttctcc gagccacgag ttgatgaaat
      961 tgcgcaggcg gtctgggaag tccttggcct gaatgaattc ggccgccacg tccccgaacg
     1021 tcagggcggg ggcatagatc gagttgaggt gaaacgccac gtggcgtggg cgttcaacgt
     1081 ccttgtcggg cacccacagc cctttaagga gcatggacat cttctgtgag tccgtgatgg
     1141 cctctccgca ccgtgagcac tcgtaccagc tagatgccct ggctcgctgg gcgacctcgc
     1201 gcagtttctc ggggtccccc ttcgcatttt tcatgtcctc ccggatctgt tccgggaact
     1261 tgatcccgct catcgtgaac ggctgcatgt agccgcagta tggacacggc acgtggtagg
     1321 cgtaccggac atcggccgaa aggtaggact gatagatggc ccccttctcc accgttgggg
     1381 tggagcactg gacgaccttc cggttggtat atgtcttcgt cctttccctt gccaggctga
     1441 ttgggtccgc ttcctcgcca aggtatggcg ggtatttgtc gacctcgtcg agccacaggt
     1501 accgtatcgg cttggaggac agggaggccg gcgagtttgc gccggacagg cagatgtagc
     1561 cgccccagaa gtggatctca agcgtggtgc tctcgctggg gatgtgacgt tccgccaccg
     1621 tggggcttgt ctcgaggagg ggctggatcc gcagcttgga gacaccacgc gcgatgtcct
     1681 ccgtgggata gaccaccatc gccgacccag ggtcctggga gactacccag ccgatctggt
     1741 tcaggatggc ctcggtcttg ccgacctgtg tccccgtcat gtacgtaatc tcgttgacat
     1801 aaggcagcgt gaagacgtcc atcattccgg caaggtaggg ggtccgtgcg ttgcgccacg
     1861 ggcccgggcg gctggatgtc cgcgtgtcaa gtacacgttc agcctcagcc cactcactca
     1921 ccgtgagcct tgccggcggc ttccacgcca tcctctcctt cgggagccac gccacgttcg
     1981 gcaggttcca cctccggcgt gtactgtccg cttctgctgt attcgttgag caggctttct
     2041 gcctcacgtc tcaataccac ctcgatccct tgcgcgtctt tcccgaccag gtcgagaggc
     2101 agggccttgg cgaagttaag aagcccgacg cgcacagcgc tcacccgtga cgcccactca
     2161 gcctcgacct cctcacgggg gagatactcg ccccgcagga tcttgagctg gagctccgcc
     2221 tgcgccgcct tggcctcccg gtagcgcgtc tcggccttcg tctgacgcct cttcaggctc
     2281 ccctcgtccg attccgcccc gtcatcctgc tcgccgctct ccgaggcgat gccgtaggat
     2341 ttaagatacc actgaacaaa atccttcaga cggtaaagcc cgtgagaatg ctgcggggca
     2401 ccgaaattgt taacgtattt tcgtagcgtg gggattgaga tgcccatgat ctctgacagg
     2461 acgtgtatgg tgacgaggaa atcggccgtt ttgatgacct ctggagcctc attttcggtt
     2521 tttccctgca tttttagcct ccaaagggct tttgccttat ttttttaggt tttttgggct
     2581 tctggccctg tttagtagcc aaaaatgatt ccaaagtgcg ggaaggcgcg gggcctccgc
     2641 cgacccctgc gcttttaccc acccgtagta cctgctgcat tgtgaagcgt cacacacatg
     2701 ctacgtgcgt acatgcatac atgtacagta aactaaaacg attataagtt tattattaat
     2761 tgtcttaatt gtaaaggaca catcgaacca ggcgccttgc gtcaatgcgc aaaactacac
     2821 tcaaatacat actctatagc tgtatcgcgt atatacgctg aatatcagtg taaagtgtgc
     2881 aatgcgaatg catgcaatga atataacgca cgtgcggtt
//
LOCUS       DAOSCR010000347         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22040_length_2912_cov_3.246412, whole genome shotgun sequence.
ACCESSION   DAOSCR010000347 DAOSCR010000000
VERSION     DAOSCR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22040_length_2912_cov_3.246412"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..694)
                     /locus_tag="PLT69_08660"
     CDS             complement(170..694)
                     /locus_tag="PLT69_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOJ14547.1"
                     /translation="MSSRMIVSLSVSALLHGLILAQPIVIFAKKVLEEAPPVRVRLVS
                     IPQEFLAVPKDGGARPERDQEPALEEGVSFTAEGGVATGYLDRLKIKIFKVWEYPEEA
                     ILRGEEGKVSIEFVLNEKGELVDIGVVKSSGSPRLDKAALEAVAEAQPFGPLEETGVG
                     KTLKVTGLFAYVLD"
     gene            1133..1230
                     /locus_tag="PLT69_08665"
     tRNA            1133..1230
                     /locus_tag="PLT69_08665"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1167..1169,aa:Sec,seq:tca)
     gene            complement(1714..2592)
                     /gene="ccsB"
                     /locus_tag="PLT69_08670"
     CDS             complement(1714..2592)
                     /gene="ccsB"
                     /locus_tag="PLT69_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOJ14548.1"
                     /translation="MSATVFSVVTLAYLASFVLFAIRASSEKDTWHRAATMVALAGLA
                     GHTGGIVLRWVESYRQGMAHVPLSNFYESLVFFSWSVTLFFALTARSRARSRVGAFAM
                     PIAFLLLAYASFSPGVDPSIRPLVPALKSDWLVMHVVTCFLGYAALLLASLTGILALR
                     EKAVQKGEPSASGEVEPAPGPRNGLQPAGRKDTDPGLETFDVFLRSGYILFTLGIVTG
                     SVWGHMAWGRYWGWDPKETWALITWLVYTCVLHARAGRALPRRKHVIVLLAVGLVCIL
                     FTYFGVNYLPGLHSYA"
     gene            complement(2589..>2912)
                     /locus_tag="PLT69_08675"
     CDS             complement(2589..>2912)
                     /locus_tag="PLT69_08675"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOJ14549.1"
                     /translation="PGITRQVPQFDPAGVPPFVFSLDGVSWKVSTVLFVNRDPGVWFA
                     GFGAVFFIAGVMLVFFVPHEQVWAVLEPEGGRLRLKVAMRRNGRAVEVPGELLERVTA
                     AGGGR"
BASE COUNT          563 a          930 c          957 g          462 t
ORIGIN      
        1 gggggggggg gggggaagcg cctgctcccg ggccacgccc tccagaacgc accttaccgc
       61 ccggcctgaa cgtcaggctg gggaagtctt tcgtggttgc gttttgccgt tgcggccaat
      121 ggcgagagca tgagcgcgcc gtcacgaaag ggaaaccggc cgaacggtct caatcgagga
      181 cgtaggcgaa cagcccggta accttgaggg ttttcccgac gccggtctct tcgagggggc
      241 cgaagggctg cgcctccgcg acggcctcga gcgcggcctt gtcgagcctg ggcgagcccg
      301 agctcttcac gacgccgata tccacaagct cgcctttctc gttcaagacg aactcgatgc
      361 tcaccttgcc ttcctcaccc ctcaggatcg cctcttcggg gtattcccat accttgaaga
      421 tctttatctt gagccggtcc aggtaaccgg tggcgacacc gccctcggcg gtgaagctca
      481 ccccttcctc cagggcgggt tcctggtctc tctcgggccg tgcccccccg tctttgggta
      541 ccgcgaggaa ctcctggggg atgctcacga gcctgaccct cacaggcggc gcctcttcca
      601 ggaccttctt tgcgaagatc acgatgggct gcgcgaggat cagcccatgg agcaaggcgg
      661 agacggacag gctcacgatc atcctcgagg acatggccac atcaaatcca cgaggccgcc
      721 cgggtgtcaa gagagaaaag gttccatgcc aagagggtac gccatgcacg aagacgacta
      781 acgggcatgg cggtgatgcg gccaggtcgg ctttttcgcc ctcccgatcc cccggaacct
      841 ggatccggcg catggtgggg ccagggtgac cgaaggtgtc acacggtctg catgcacaac
      901 gctcgtcgat tcacaagggg tatgcatacc gtgcattcag acggtgcatg agcaggaagc
      961 ggaccacgtc ccgacatgcg gtgagtgggc atcgccatcc ctcccctcag cacccatgtg
     1021 gacaccccgt cccgaaatac ggggagaggg cctaaggggg atggcgcatg aaaaagaaga
     1081 gacccacgtg acgcgttgat cggcaacggg tcaatgtggt atggtctccg gcggaagcgg
     1141 acgggtcctg gtgggccccc cggacttcaa atccggctgt ggggtgcgac gagcgtcccg
     1201 ggtgggttcg attcccatgc gcttccgcca aaaccctttc acatcactca tcatctcatc
     1261 cagcaacggt cactcggttc tcacacacca tcgaatgggt gggtgtcatg accgagatgg
     1321 gtgcttgcct cgcgacggtc gcctggatcc cgtctggttt gcggtttacg caccctgtcc
     1381 ggatagggcg gataccggga agcccggcgc tgccctgtcg agaaagacca ccctccaccg
     1441 tcgagacgat gggcgacaca gggacacccc ctctccgggg catgcatcca ggtgaggcgc
     1501 tgttcccgtg gggatggtgt tcgcgggcat cccccattcg aaaacagggg gctgagggat
     1561 gatccggatg tcgacgaggc ggcggatgac gggctcaaag agccgccccc tgtcggcgca
     1621 ggagaggaag agggggcagg gactccccgg cgtggcgtat ccagggcggg tcgtgcgcca
     1681 gggcgccagg cggtggcgcg aggggtgtcc gtctcaggcg tagctgtgga gcccgggcag
     1741 gtagttcacc ccgaagtagg taaagaggat gcagacaagc ccgaccgcca gaaggacgat
     1801 cacgtgcttc ctcctcggca gggcccggcc tgcccgtgcg tggagcacac aggtgtagac
     1861 gagccatgtg atgagcgccc aggtctcctt ggggtcccac ccccagtacc gcccccacgc
     1921 catgtgcccc cagaccgagc cggtgacgat gcccaaggtg aagaggatgt agcccgatct
     1981 caagaagacg tcgaaggtct cgagcccggg atccgtgtct ttgcgcccgg ccggttgcag
     2041 gccgttacgg gggccgggtg caggctcgac ctccccggaa gcggaaggtt cgcctttctg
     2101 caccgccttt tcacggagtg ccaggatccc cgtgagggag gccagaagga gcgcggcata
     2161 cccgagaaag caggtgacga cgtgcatgac gagccagtcg ctcttgaggg ccggcacgag
     2221 cggcctgatg ctcgggtcca ccccgggcga gaacgacgca taggcaagca gcaaaaaggc
     2281 gatgggcatg gcgaacgcgc caacccgcga cctcgccctc gacctcgcgg tcagggcgaa
     2341 gaacagggtg accgaccagg agaagaacac gagcgactcg tagaagttcg acagggggac
     2401 atgcgccatg ccctgccggt acgactcgac ccacctcagc acgatccccc ccgtgtgccc
     2461 tgcgagacca gcaagcgcca ccatggttgc cgcgcggtgc catgtgtcct tttctgaaga
     2521 cgccctgatg gcgaacagga cgaaggacgc gaggtatgcg agggtgacaa cggagaagac
     2581 cgtggcgctc acctgccacc ccctgcggcc gtgacccttt ccagcagttc cccggggacc
     2641 tccacggccc tgccgtttct cctcatggcc accttgagcc tgagacggcc gccctcaggc
     2701 tcgagcaccg cccagacctg ctcgtgggga acgaagaaca cgagcatgac gcccgcaatg
     2761 aagaaaacgg cgccgaagcc tgcgaaccag acgccggggt ccctgttcac gaagaggacc
     2821 gtggagacct tccaggacac cccatccagc gagaagacga aaggcggcac gcctgcggga
     2881 tcgaattggg ggacctgcct ggtgatgccc gg
//
LOCUS       DAOSCR010000348         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22049_length_2911_cov_3.882703, whole genome shotgun sequence.
ACCESSION   DAOSCR010000348 DAOSCR010000000
VERSION     DAOSCR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22049_length_2911_cov_3.882703"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PLT69_08680"
     CDS             <1..1690
                     /locus_tag="PLT69_08680"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOJ14550.1"
                     /translation="GKVVVSCTINDTGRLTLDSSFTLKPFTADTAISMDDLQPAWVQP
                     YVMERVPILIRRGTLSARGRVRLARDGDRPLGLHFTGDVRINDFASVDRAHAEDLLSW
                     KDLSITGIDLTLNPGRLAIAEIELAGASGAFIVNPDGSSNVPTLAGEKKATLKHAEGG
                     RKKAMERVAVGRVRVKNGRFTFTDRTVTPKYTSSLTAINGTVTGLSSDVFRKAVVNLQ
                     ARLDNQAPIAITGSINPLKKDLFVDLKATLKNMELSPVTPYSGTYAGLAIEKGKLSLD
                     LDYLIDRKRLNAKNDVIIDQLTFGEAVPSEKATRLPVRLAVALLRDSSGRIDLHLPVT
                     GRTDDPEFHIGRLVLKMIVNILEKAATSPFALLEAMYPGATELSHITFEPGRSTLTDD
                     ARKKLSDLAKILTEKPSLTLEITGFVDASSDREGLAKTLFERKLKAQKIKDLLKAGKQ
                     ASSVDEISLEPAEYGTYLAKAYRAESFDKPTNVLGIPKTLPDEEMKLLIMEHIKVTDD
                     DLKALAAARSRQVRDELAHAWQVDPGRIFLVEADPFKPEQIDKVANSRVSLTIK"
     gene            1831..2601
                     /locus_tag="PLT69_08685"
     CDS             1831..2601
                     /locus_tag="PLT69_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOJ14551.1"
                     /translation="MAYETLLLEKQGHITVLTLNRPPTNSVSYAMLGEIDKALDEVAA
                     DSDARVLVITGAGERCFSAGMDVNDSLSHPDVGEVGRALWTKVDLFDKPTIAAVNGHA
                     LGGGCELALACHFRYMADNPKAAIGCPEINLGIIPGWGGTQRMTRLLGRSKALDLMLN
                     AKRLSPQEALAAGLVDAVFDQAGFMEKVMEIAASLAERAPLSARGIIRAVNAYCYQGI
                     EKGLEAEAASSKICASSKDAIEGFTAFMQKRKPEFKGE"
     gene            2617..>2911
                     /locus_tag="PLT69_08690"
     CDS             2617..>2911
                     /locus_tag="PLT69_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOJ14552.1"
                     /translation="MEVKKIAVIGAGAMGNGIAQVGIMAGFDVAMQDIEQRFVDRGVA
                     TIKDSLAKFVGKGKITPEQSDDMLKRLIPTTDLAAAVKDADLVIEAVFEDLDLK"
BASE COUNT          636 a          927 c          891 g          457 t
ORIGIN      
        1 cggcaaggtg gtcgtgtcct gcacgatcaa cgataccggc aggcttaccc tcgattcctc
       61 gttcacgctc aagcccttca ccgcggacac ggccatcagc atggacgacc tgcagcccgc
      121 atgggtgcag ccatatgtca tggaaagggt gccgatcctc atcaggcggg gaacgctgag
      181 cgccaggggc cgcgtgcggc tggccaggga cggtgaccgt cccctcgggc tgcacttcac
      241 cggcgacgtc cgcatcaacg acttcgcatc cgtggacagg gcgcatgccg aggacctcct
      301 ctcgtggaag gatctgtcca tcacgggcat cgacctcacc ctgaacccgg gacgcctggc
      361 catagccgaa atcgaacttg ccggggcttc gggcgccttc atcgtcaacc cggacggttc
      421 cagcaacgtc cccaccttgg cgggagagaa aaaggccact ttgaagcacg ccgaaggcgg
      481 ccggaagaag gccatggagc gcgtggccgt gggcagggtg cgcgtgaaga acggccgctt
      541 caccttcacc gaccgcacgg tgaccccgaa gtacaccagc tcgctcaccg ccataaacgg
      601 caccgtcaca ggcctttcct cggacgtgtt caggaaggcc gtggtcaatc tccaggcacg
      661 gctcgacaac caggcgccca tcgccatcac cgggtccatc aacccgttga agaaagacct
      721 gttcgtggac ctgaaggcga ccctgaagaa catggaactg agccccgtga ccccctactc
      781 gggcacatat gcaggcctgg ccatcgagaa gggcaagctt tccttggacc tggactatct
      841 catcgacaga aaacggctga acgcgaaaaa cgacgtcatc atcgaccaac tcaccttcgg
      901 ggaggctgta ccgagcgaga aagccaccag gcttccggtg agactcgccg tggcccttct
      961 cagggacagc agcggcagga tcgacctcca ccttcctgtc acgggccgaa cggacgaccc
     1021 ggaatttcac ataggaaggc tcgtcctcaa gatgatcgtg aacatcctgg aaaaggccgc
     1081 cacctcgccc tttgccctgc tggaggccat gtacccgggc gcgacggaac tgagccacat
     1141 caccttcgag ccgggcaggt ccacgctcac ggacgatgcg cggaagaaac tctcagacct
     1201 cgcgaagatc ctcaccgaaa agccttcgct caccctcgag ataacggggt tcgtggatgc
     1261 atcgtcggac agggagggcc ttgcaaaaac cctcttcgag cgcaagctca aggcccagaa
     1321 gatcaaggac ctgctgaagg cggggaagca ggcatcgtct gtggacgaaa tcagcctgga
     1381 gccggccgaa tacggcacct acctcgccaa ggcctacagg gccgaatcgt tcgacaaacc
     1441 cacgaacgtt ctcggcatcc ccaagaccct gccggacgag gaaatgaagc tgctcatcat
     1501 ggagcacatc aaggtaacgg atgacgacct caaggccctt gccgcggcca ggtcccggca
     1561 ggtcagggac gagctcgccc acgcgtggca ggtggaccct ggacggatct tccttgtgga
     1621 ggcagacccc ttcaagcccg aacagattga caaggtcgcg aactcccggg tgagcctcac
     1681 catcaagtga acagtgatca cgcttttatt cgaacgagcg ctcacttttt ttattggcat
     1741 tccatcgcac gatcatgtat actaggaggg caatgttcgg taccagccct gatggtaact
     1801 ctattaccct tatggatcaa aggaggctct atggcgtatg aaacccttct cctggaaaaa
     1861 caaggtcata tcacggtgct caccctgaac cggcctccca ccaactcggt gagctacgcc
     1921 atgctgggcg agatcgacaa ggctctggac gaggtggcgg cagacagcga cgcccgcgtg
     1981 ctcgtcatca ccggcgccgg ggagaggtgc ttctcggccg gcatggacgt caacgactcg
     2041 ctctcgcacc ccgacgtggg cgaggtgggc cgcgccctgt ggaccaaggt ggacctgttc
     2101 gacaagccca ccatcgccgc cgtcaacggc cacgcgctcg gcggcgggtg cgagctcgcg
     2161 ctcgcgtgcc acttccggta catggccgac aaccccaagg ccgccatcgg gtgcccggag
     2221 atcaacctgg gcatcatccc gggctgggga gggacccagc gcatgaccag gcttttggga
     2281 aggagcaagg cgctcgacct catgctcaac gcgaaacgcc tgagcccgca ggaggccctt
     2341 gccgcagggc tcgtcgatgc ggtcttcgac caggccgggt tcatggaaaa ggtcatggag
     2401 atcgcggcct cgctcgcgga gcgcgcaccc ctctcggcaa ggggcatcat ccgtgcggtc
     2461 aacgcgtact gctaccaggg gatcgagaag ggtctcgagg ccgaggcggc gtcgagcaag
     2521 atctgcgcct cgtccaagga cgcgatcgag gggttcaccg cgttcatgca gaagagaaag
     2581 cccgagttca agggcgagtg aagaaggagg gggaccatgg aggtgaagaa gatagcggtc
     2641 ataggggcgg gcgccatggg caacgggata gcccaggtcg gcatcatggc aggcttcgac
     2701 gtggccatgc aggacatcga gcagcgcttc gtcgacaggg gggtggccac catcaaggac
     2761 agcctcgcaa agttcgtggg caagggcaag atcacccccg agcaaagcga cgacatgctc
     2821 aagcggctga tccccaccac cgacctcgcg gcagccgtga aggacgccga cctcgtcatc
     2881 gaggccgtgt tcgaggacct ggacctcaag a
//
LOCUS       DAOSCR010000349         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22113_length_2906_cov_2.938969, whole genome shotgun sequence.
ACCESSION   DAOSCR010000349 DAOSCR010000000
VERSION     DAOSCR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22113_length_2906_cov_2.938969"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLT69_08695"
     CDS             <1..617
                     /locus_tag="PLT69_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOJ14553.1"
                     /translation="REGREYLGAMAASANFAFANRAIVAYLVRRAFERVFGLSFERLG
                     MRMLVDCCHNVAKVERVEWEGRVVEACIHRKGATRALPAGDVRLPARYAVTGQPVLVP
                     GDMGRMSFILCALDGARETFFSACHGAGRLLSRHEAKRVSRGRKILDELRHKGIRAMA
                     ASKATLAEEIPDAYKDVSDVVDTVSGAGIARPVARLRPFAMVKG"
     gene            complement(619..1257)
                     /locus_tag="PLT69_08700"
     CDS             complement(619..1257)
                     /locus_tag="PLT69_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOJ14554.1"
                     /translation="MTAQERFTLEGKTRELRKKKVRALRRQGVLPGVIFGKDVEPLHV
                     QVDEHDFELLYRKAHKTSIVTVDVDGKKTDVLIHSIHRDKITGRPLHVEFLKVDLKRQ
                     VTVEVPLVFVGVSPAEKSGLGKITHEETSIHLRCAPDRIPESIEVDVSVIKSKHDVIH
                     AADLKLPEGVHLGHGVSDRKVIASLVSARVAEAAPETVEAEAAEGTEAAQEG"
     gene            1611..2084
                     /gene="greA"
                     /locus_tag="PLT69_08705"
     CDS             1611..2084
                     /gene="greA"
                     /locus_tag="PLT69_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ14555.1"
                     /translation="MKRNPITREGYAALREELERLKKHERPKIIAAIEEARGHGDLSE
                     NAEYIYAKERQAFIEARMQDLENKLGNAEIVDTSRLPTDRIVFGSTFVLVNVDTGEES
                     RYQIVGVDESDISKGKISVESPMAKALIGKRVSDEVVVETPAGRREFEVVEIVSG"
     gene            complement(2074..2799)
                     /gene="tatC"
                     /locus_tag="PLT69_08710"
                     /pseudo
     CDS             complement(2074..2799)
                     /gene="tatC"
                     /locus_tag="PLT69_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
BASE COUNT          516 a          936 c          964 g          490 t
ORIGIN      
        1 gcagggaggg cagggagtat ctcggggcca tggccgcctc ggccaacttc gccttcgcga
       61 accgggcgat cgtggcgtat ctcgtgaggc gcgcgttcga gcgcgtcttc ggcctctcct
      121 tcgagaggct cggcatgcgc atgctcgtgg actgctgcca caacgtggcc aaggtcgagc
      181 gggtggagtg ggagggccgg gtggtggagg cctgtatcca ccgcaagggc gccacgaggg
      241 ccctgcccgc aggcgatgtg aggctgcccg cgaggtacgc ggtcacgggc cagcccgtgc
      301 tcgttcccgg cgacatgggc aggatgtcct tcatcctgtg cgccctcgac ggcgccaggg
      361 agaccttctt cagcgcctgc cacggcgccg ggaggctgct gtcgaggcac gaggcgaaga
      421 gggtatccag gggcaggaag atcctggacg agctcaggca caaagggatc cgggccatgg
      481 cggcctcgaa ggccacgctc gcggaagaga tcccggacgc ctacaaggac gtttccgatg
      541 tcgtggacac ggtctcgggc gcgggcatcg ccaggcccgt ggcccggctc aggccgttcg
      601 ccatggtcaa ggggtgagtc agccctcctg ggctgcttcg gtcccctcgg cggcctccgc
      661 ctccacggtc tccggcgcgg cctcggcgac gcgcgccgag acgagcgatg cgatcacctt
      721 gcggtcgctc acgccgtggc cgaggtggac gccctctggg agcttgaggt cggccgcgtg
      781 tatcacgtcg tgcttgctct tgatgaccga aacgtccacc tcgatggact ccgggatcct
      841 gtccggggcg cacctgaggt ggatggaggt ctcctcgtgg gtgatcttgc ccagcccgga
      901 cttctcggca ggggagaccc cgacgaagac gagcggtacc tcgacggtga cctgcctctt
      961 caagtccacc ttgaggaact ccacgtgcag gggcctgccg gtgatcttgt cccggtgtat
     1021 ggagtggatc aggacgtcgg tcttcttccc gtcgacgtcg acggtcacga tggaggtctt
     1081 gtgggccttg cggtacagca gctcgaagtc gtgctcgtcg acctgcacat ggaggggttc
     1141 cacgtccttg ccgaagatca ccccgggcaa gacgccctgc cttctcagcg ccctcacctt
     1201 cttctttctc agctcccgcg tcttaccttc gagcgtgaac ctttcctgtg cagtcatggt
     1261 ctcctccggg aatgcagtag agtgtcccta tagcggcaaa cgccacgcca agtcaatgcc
     1321 caactccctt gccccacccc ccgggacccc gccgtgccat gcgtggtggg tgcgccgcgc
     1381 acgaggaaac cccttccctg ggatccgctc gcgtccccgg aagtcaccgc agagcacagg
     1441 cccccgggag agtgccgcgc cccttccccg tgcccggtac gtgggctgcc ccgtcaccac
     1501 ccggcccgtt ttcccttgac acacaggccg ccttttgtcg ataatcccgt catcgatact
     1561 ggtagtgccc taccggtatc tttttctttt gtgaggaagc gaacatggcc atgaaacgca
     1621 accccataac ccgggaagga tacgcggcgc ttagggagga gctcgagagg ctcaagaagc
     1681 acgagaggcc caagatcatc gcggccatag aggaggcacg cggccacggc gacctgtccg
     1741 agaacgcaga gtacatctac gccaaggaac gccaggcctt catcgaggcg cggatgcagg
     1801 atctcgagaa caagctcggc aacgcggaga tcgtcgacac gagcaggctt cccacggacc
     1861 gcatcgtgtt cgggtccacc ttcgtgctcg tcaacgtcga cacgggcgag gagagccgct
     1921 accagatcgt gggggtggac gagtccgaca tctcgaaggg gaagatctcg gtcgagtccc
     1981 ccatggcaaa ggccctcatc ggcaagaggg tgtcggacga ggtggtggtg gagacgcccg
     2041 ccggcaggcg cgagttcgag gtcgtggaga tcgtcagcgg ctgacgaccg gctccgcctc
     2101 gcgcttcccg aagaggtacg tcaccgcgac gctcagttcg aacagcgcgt agagcggcac
     2161 acccaagagg gccatgctga gcacgtccgg cggggtcagc accgcagccg ctatggcgat
     2221 gatcaggatc gcgtacttgc ggttcttcgt cagcgtcctg tgggtgacga cacccatcct
     2281 cgccaggaag aacatgacca ccggcagctc gaaggtgacc ccgaaggcga tgatgaggct
     2341 catgcacagg ctcaggtact ccttcatcga gggcagcgcc cggatgtggt ccgtggaaaa
     2401 gctgaggaag aacctgaagc cgaaggggaa ggccacgaag aacgcgaagg acgcccccaa
     2461 gagaaagaac aagatcgccg cggccatgaa ggggtgcacg tacctccgct agttcgggta
     2521 gagccctggc atcacgaact tccacgcctg gtagaagacc accggcgccg cgaggaagag
     2581 accggcgaag aaggacacct tgaggtaggt gacgaacgcc tcctggagcg aggtgtagat
     2641 gagggtgctc ccttcgggca tggcctgcac gagcggccag gtgagcaggt cgaagatccg
     2701 gctcgcgaag gagtaggaca gggcgaagcc gacgccgatc gcggccaggc acttgaggat
     2761 cctccgcctg agctcctcga ggtgctcggt gatgggcatc cgttcctcgt ccacgcgcct
     2821 tctccccgag ccccgctcac tcggcggcct tctgtgggtc gtccctctcc ggccgtctct
     2881 cgtcgtcctt cttcgcttcc ggcgag
//
LOCUS       DAOSCR010000350         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22171_length_2900_cov_3.460105, whole genome shotgun sequence.
ACCESSION   DAOSCR010000350 DAOSCR010000000
VERSION     DAOSCR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22171_length_2900_cov_3.460105"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLT69_08715"
     CDS             complement(<1..1059)
                     /locus_tag="PLT69_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ14556.1"
                     /translation="MAADVVMFSNIPVVLTYSVPPGMEAMPGCRVTAPVRSTLRVGVV
                     IEVREALEEVVPLKDIHSVLDDRPLLTPELIGLVRWTSGYYHAGIGPCMALAFPPALR
                     RARGVVLEEDPVLVKTGVTPSRLGSVQKKVLDAVGDGGVLMSELAKRFPGSSSVIRGL
                     VAKGALHALAARKGDGGPRGEPAPEHSPDQRRAIQEICAAIEKGGFRAFLLHGITGSG
                     KTEVYLSCALEALRRGRSVLYLVPEIALTAQTVAMIRRRIPEEIAVFHSGLPEIERAR
                     AFLRVAQGGVRFVLGTRSAVFAPLTGIGLVVVDEEHDHSYKQDEGVPYNARDLGVLRA
                     SNHGACVVLGSATPSLESY"
     gene            1183..1839
                     /locus_tag="PLT69_08720"
     CDS             1183..1839
                     /locus_tag="PLT69_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ14557.1"
                     /translation="MKGIALDQMLKDLLDPDPTIKKMAARAILRESDDPGQVLAGALD
                     GADTQTLEAVLDVLFNSDGDFTEIFSGMTRHEDASIRAKAIRYLFRKGAFTPADAVAW
                     LEDADPYVRRRAISYLFWMNDAPSLASVSRMCTTDPDESVRKDCLRLLSIWGGEKDLE
                     HVIKALEDESFEVRLQAVQTLRRLTGEDFGDPAGAAPDEFEWIVAKWQGWWELTRKQQ
                     "
     gene            1844..2803
                     /locus_tag="PLT69_08725"
     CDS             1844..2803
                     /locus_tag="PLT69_08725"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14558.1"
                     /translation="MEQPVYTAKDFATLLGITKPQLLKMENEGILPAPRTENGVRVYY
                     PHDIPLYLRLLGKPQLVSSKRRQIFLNFKGGTGKTSISAFYAYRLACLGMKVLLVDLD
                     PQGHLTQCLGIDHESFEATLYNVLIERTDIRDVIMPTRMENLKLVPANLDLSPVELAL
                     TSMNAREQRLRKALAPIQDEYDVIVMDASPSIGLLNLNGILASNDLFVPVLADFLSYH
                     GLKILFETLATIEEDFDFIFENIYIFLNNYNASHNICQRAKEALQTHYDGYLMKTIIR
                     QDIKIAEAASVGMPIHQYAPKNRGSVDLNEFIKEVFPNLPIVF"
BASE COUNT          548 a          978 c          897 g          477 t
ORIGIN      
        1 gtagctctca aggctcggcg tcgccgagcc gagcacgacg caggcgccgt ggttcgaggc
       61 cctcagcacg ccgaggtccc gcgcgttgta cgggacgccc tcgtcctgct tgtagctgtg
      121 gtcgtgctcc tcgtcgacca ccacgagccc tatgcccgtg aggggggcga acacggcgct
      181 gcgcgtgccg aggacgaacc tcacccctcc ctgggccacc ctgaggaacg cccgcgcccg
      241 ctcgatctcg ggcagccccg aatggaacac ggcgatctcc tccgggatcc tcctcctgat
      301 catggccacg gtctgggcgg taagcgcgat ctccggcacc aggtagagca ccgatcgccc
      361 cctcctgagc gcctcgaggg cgcaggagag gtacacctct gtcttgccgg aacccgttat
      421 cccgtggagc aggaaggcgc ggaagccgcc cttctctatg gccgcgcata tctcctggat
      481 cgcccggcgt tggtcgggcg aatgctcggg cgcgggctcg ccccgggggc ctccatcccc
      541 tttccgtgcg gccagggcat gcagcgcgcc cttcgccacg agccccctaa tcacggagga
      601 tgagccgggg aagcgcttgg cgagctcgct catgagcacc cccccgtcac cgacggcgtc
      661 cagcaccttt ttctggaccg agcccagccg cgagggggtg accccggtct tgacgagcac
      721 cgggtcttcc tcgaggacga cgcccctggc cctcctgagc gccggcggga aggcgagcgc
      781 catgcagggg cctatccccg cgtggtagta gccggacgtc cacctgacga gccctatgag
      841 ctcgggggtc aggaggggtc ggtcgtcgag cacggagtgg atgtccttga ggggcacgac
      901 ctcctcgagg gcctccctga cctcgatcac cacccccacc ctgagggtag agcgcacggg
      961 cgccgtcacc ctgcacccgg gcatggcctc catgcccgga ggtacgctgt aggtgagaac
     1021 cacggggatg ttcgagaaca tgacgacatc ggcagccatg cgcgccatac accaagccta
     1081 ccacgggcag gtgacccatg gaagccctct cgtggtcgtg cttgactctc ctcgaccttc
     1141 acccgtatat tcgagaacac ggagttttga gggggcctct tcttgaaggg tatcgccttg
     1201 gaccagatgc tgaaggatct gctcgacccg gaccccacca tcaagaagat ggcggcccgg
     1261 gccatcctca gggagtccga cgacccagga caggtgctgg cgggggcgct cgacggggcc
     1321 gacacgcaga ccctcgaggc cgtgctcgac gtcctgttca actccgacgg cgacttcacc
     1381 gagatcttct ccggcatgac caggcacgag gacgcctcca tacgcgccaa ggcgatcagg
     1441 tacctgttca ggaagggcgc gttcaccccg gccgacgccg tggcctggct cgaggacgcg
     1501 gacccgtacg tgaggaggag ggccatcagc tacctcttct ggatgaacga cgccccgtcg
     1561 ctggcctcgg tctcgaggat gtgcaccacc gacccggacg agtcggtgag gaaagactgc
     1621 ctgcgcctgt tgtcgatctg gggaggcgag aaggacctgg aacacgtgat caaggcgctc
     1681 gaggacgagt ccttcgaggt gaggctccag gcggtgcaga ccctcaggag gctcaccggc
     1741 gaggacttcg gcgaccctgc cggggccgcc cccgacgagt tcgagtggat cgtggccaag
     1801 tggcagggct ggtgggagct gacgaggaag cagcagtaga cgcatggaac agccggtata
     1861 cacagcaaag gatttcgcga cgctcctcgg gataaccaag ccgcagctcc tcaagatgga
     1921 gaacgagggg atcctgcccg cgcccaggac ggaaaacggg gtgcgcgtgt actacccgca
     1981 cgacatcccg ctctacctca ggctcctggg taagccgcag ctggtctcgt cgaaaaggag
     2041 gcagatcttc ctcaacttca agggcggcac gggcaagacg agcatctcgg cgttctacgc
     2101 ctacaggctc gcctgcctgg gcatgaaggt cctcctcgtc gatctcgacc cgcagggcca
     2161 cctcacccag tgcctgggca tcgaccacga atccttcgag gccaccctct acaacgtgct
     2221 catcgagcgc acggacatcc gcgacgtgat catgcccacc cgcatggaaa acctgaagct
     2281 cgtccccgcg aacctggacc tgtcgccggt ggagctcgcg ctcacgtcga tgaacgccag
     2341 ggagcagcgc ttgagaaagg cgctcgcccc catccaggac gagtacgacg tcatcgtgat
     2401 ggacgcctcc ccgtcgatcg ggctcttgaa cctgaacggc atcctcgcga gcaacgacct
     2461 cttcgtgccc gtgctggccg acttcctgtc ctaccacggg ctcaagatcc tcttcgagac
     2521 cctggccacc atcgaggagg acttcgattt catcttcgag aacatctaca tcttcctgaa
     2581 caactacaac gcgtcgcaca acatctgcca gcgtgccaag gaggccctcc agacccacta
     2641 cgacggctac ctcatgaaga cgatcatccg ccaggacatc aagatcgcag aggcggcgag
     2701 cgtgggcatg cccatccacc agtacgcgcc caagaacagg ggctcggtgg acctcaacga
     2761 gttcatcaag gaagtcttcc ccaatctccc gattgtgttt tgaccggttt tgtgcttaat
     2821 ggaggtggtg cctcctgggg aggtcccttg tattcaatga ctccggaaat tgacatgagg
     2881 agcaactacc catgaaagac
//
LOCUS       DAOSCR010000351         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22282_length_2890_cov_3.702998, whole genome shotgun sequence.
ACCESSION   DAOSCR010000351 DAOSCR010000000
VERSION     DAOSCR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22282_length_2890_cov_3.702998"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLT69_08730"
     CDS             complement(<1..911)
                     /locus_tag="PLT69_08730"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOJ14559.1"
                     /translation="MIGRVRGGGAEFAATALYLLYGCLVVALPALTIPLALFCPSLWC
                     ILASGTSTRRAFLAGAAPLALAVLPWAGQGAAVYALFYVAAMLMHSSMNRSMTALAVI
                     APTVLAASVIASAMLVSAHAEGASLGDVVSRWVQEVMKGSRDAADAMLYGAELEEFKA
                     MLETARSRIVTLFPGLVLTGCAFVFWVNLVIANAKTHRVGLRTYRVPDALIACFIAAG
                     TLSVLPWEKLSAVGLNLLLVVGFVYFLQGLAIASVFMDSRGWPAFIRSAIYILIFIQI
                     YMVIVVAAVGLFDAWFDFRARITTRKG"
     gene            complement(916..1176)
                     /gene="rpsR"
                     /locus_tag="PLT69_08735"
     CDS             complement(916..1176)
                     /gene="rpsR"
                     /locus_tag="PLT69_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOJ14560.1"
                     /translation="MQDRPPRSQSNPRQKPYYRRKTCRFCEDKTLPLDYKAPHVLKDF
                     ITERGKILPRRITGTCARHQKSLSTQIKRARIMALLPFTVHD"
     gene            complement(1166..1570)
                     /gene="rpsF"
                     /locus_tag="PLT69_08740"
     CDS             complement(1166..1570)
                     /gene="rpsF"
                     /locus_tag="PLT69_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOJ14561.1"
                     /translation="MNLYEVMFILDPDMGEEEREKVLARFKSAISKNKGDLIRIDDQG
                     LKSLAYKINKKSRGHYHLAYLEGPGAMVAEIERLLRIDENVMRFVVVRQEEHVTRADL
                     ERTASVEEKAEEKTPGGEEIPEQAETGADNAG"
     gene            complement(2160..2435)
                     /locus_tag="PLT69_08745"
     CDS             complement(2160..2435)
                     /locus_tag="PLT69_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOJ14562.1"
                     /translation="MKAFRLTIRGRVQGVWYRATARETAQGLGIRGWVRNAEDGSVEA
                     HIQGDDESLERMIRWCRTGPPGARVEEVLVEEAEPVDEYRSFSIRYY"
     gene            complement(2440..>2890)
                     /locus_tag="PLT69_08750"
     CDS             complement(2440..>2890)
                     /locus_tag="PLT69_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ14563.1"
                     /translation="SVASRDLALAFKGMERFMQAGVQELTSVQGVGEETARAIRDFFE
                     NSENVKEITRLFASGVAPAEPAGPRTAGGALAGKSVCFTGTLSSMTRSQAKAMAEAHG
                     ARVLDGVSKNLDYLVAGKDPGSKVDKARSLGVEIIDEDRFLNMIQEA"
BASE COUNT          501 a          967 c          895 g          527 t
ORIGIN      
        1 tctccttttc gggttgttat cctcgccctg aaatcgaacc acgcgtcgaa gagcccgacc
       61 gcggcgacga cgatcaccat atatatctgg atgaagatta agatgtaaat agccgagcgg
      121 atgaacgccg gccaacccct cgagtccatg aacaccgacg ctatggcgag cccctggaga
      181 aagtacacga agccgaccac gagaaggagg ttgaggccga cggccgagag tttctcccac
      241 gggaggacgg acagggtccc cgctgcaatg aagcaggcta tgagcgcatc gggtacccga
      301 tacgtcctga gacccacgcg gtgggtcttg gcgttcgcga tgacgaggtt gacccagaag
      361 acgaacgcac aacccgtgag gacgaggccc gggaacaggg tgacgatcct cgaccgggcg
      421 gtctcgagca tggccttgaa ctcctcgagt tccgcgccgt agagcatcgc atcggcagca
      481 tccctggaac ccttcatcac ctcttggacc cacctcgaga cgacatcccc gaggcttgcc
      541 ccctctgcat gggcggagac gagcatcgcg gaggcgatca ccgaggcggc gaggaccgtc
      601 ggtgcgatca ccgcgagtgc ggtcattgac ctgttcatgg aggaatgcat gagcatggcc
      661 gccacgtaga agagcgcgta caccgcggca ccctggccgg cccacggcag caccgcgagt
      721 gcgagcggcg ctgcgcccgc caggaaggct ctgcgggtgg acgtcccgct tgcaaggatg
      781 caccagaggg acgggcagaa gagcgcaagc gggatcgtca aggcgggcag ggccaccacg
      841 agacaaccgt agagcagata gagcgccgtt gcggcgaact cggcgccccc gccccggact
      901 ctgccgatca tggcttcagt cgtgcacggt gaacgggagg agcgccatga tccgcgcgcg
      961 cttgatctgg gtggacagac tcttctggtg cctggcgcag gtaccggtga tcctgcgggg
     1021 aaggatcttc ccccgctcgg ttatgaaatc cttgagtaca tgcggggcct tgtagtcgag
     1081 cgggagagtc ttgtcctcgc aaaacctgca cgtcttgcgg cggtagtacg gcttctgcct
     1141 cgggttcgat tgcgatctcg gcggcctatc ctgcattgtc tgctcctgtc tctgcctgtt
     1201 cgggaatctc ttccccaccc ggtgtcttct cttcggcctt ttcctccacg gacgccgtcc
     1261 gctcgagatc agccctggtc acgtgttcct cctggcggac gacgacgaac ctcatgacgt
     1321 tctcgtcgat cctgaggaga cgctcgatct cggccaccat ggcgccgggg ccttcgagat
     1381 aggcgagatg gtagtgtcct ctcgatttct tgttgatctt gtaggcaagc gacttcaggc
     1441 cctggtcatc gatgcggatc aagtcgccct tgttcttgga gatcgcggac ttaaagcgcg
     1501 ccaggacctt ttccctttcc tcttcgccca tgtcggggtc gaggatgaac atgacctcgt
     1561 acaggttcat cgatccctcc ttctggtttt ccagccccgc gtggcggagc aaggagttgg
     1621 aactccatat accaatccgt gtgcaagcgc aaggaggaaa tgcctcacgg ccccatgcgc
     1681 cccgtgtcgg cgattgtccg cgtacggcca ggctcgaggc gctcagacga accttctcga
     1741 taccccctgc cccaggtgtc cgctaacgca tgaaaaagac gccctgccca cgcacgaagc
     1801 gagcccgcct agacctgcct cgcaggtccc ctgggggcat catgcacgcg ccggccgaac
     1861 tgaggtgaca acggggccgt ccacgcgccg ggaagaaagc aggccggttg tccacgaggc
     1921 cttccggcga ggctttccct tcgggccagg cattcttctg ctgccgtcca cggctggcgt
     1981 gcccctcggt gggaacgcgc cttttcgcgg cactcccagg tgacacggcg ctctccttcg
     2041 ggcccgagtt gccgcacggc ctcgagacat cgcccctcgg ggggcaggaa ggcacctcgt
     2101 cgtctccgtc cgggcatggg tgtccaccag gcgggcgcat tcctcgggca tggcctgtct
     2161 cagtagtacc tgatggaaaa cgagcggtac tcgtccacgg gctcggcctc ttccacgagc
     2221 acttcctcga cccttgcgcc gggcgggccg gtgcggcacc aacggatcat cctctcgaga
     2281 ctctcgtcgt cgccctggat gtgcgcctcc accgacccgt cctcggcgtt acgcacccag
     2341 cccctgatac ccaggccctg ggcggtctcc cgggccgtgg cccggtacca caccccctgc
     2401 accctccccc tgatggtgag acggaaggcc ttcatgctct caagcctcct ggatcatgtt
     2461 caagaaccgg tcctcgtcga tgatctcgac gccgagcgaa cgggccttgt cgaccttcga
     2521 gccggggtcc ttgcctgcga cgaggtagtc gaggttcttc gagacaccgt cgagcacccg
     2581 ggcgccgtgc gcctcggcca tggccttggc ctgcgagcgc gtcatggacg agagggtgcc
     2641 ggtgaaacag acggacttgc ccgcaagcgc gcccccggcg gtccggggcc cggcgggctc
     2701 ggcaggcgcc accccgctcg cgaagagccg ggttatctcc ttgacgttct cgctgttctc
     2761 gaagaagtcc ctgatggcac gggcggtctc ctcccccacg ccctgtaccg aggtgagttc
     2821 ctgcacgcct gcctgcatga acctctccat gcccttgaac gcaagagcga ggtcccgcga
     2881 ggcgacgctc
//
LOCUS       DAOSCR010000352         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22393_length_2879_cov_3.235127, whole genome shotgun sequence.
ACCESSION   DAOSCR010000352 DAOSCR010000000
VERSION     DAOSCR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22393_length_2879_cov_3.235127"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..1032
                     /locus_tag="PLT69_08755"
     CDS             487..1032
                     /locus_tag="PLT69_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOJ14564.1"
                     /translation="MDRPVSIHDIRLLAENIGEAGLERDITVSPADIQVVLSDVDVSV
                     DEPFSVHYEVVRHQETFHVQVDIQGCIHATCARCLAPMDHRVDLHLESDYVPAPSDMT
                     GDLEAQRVTAETGFYDRDIHLGKYICSELVLSLPYIYLCSETCKGMCPRCGANLNEGP
                     CLCAKEVDPRLEVLGSLKTTR"
     gene            1058..1243
                     /gene="rpmF"
                     /locus_tag="PLT69_08760"
     CDS             1058..1243
                     /gene="rpmF"
                     /locus_tag="PLT69_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOJ14565.1"
                     /translation="MPLPKRRHSSARRDKRRANDFAPTPAVSFCPNCNEPKAPHRVCP
                     SCGYYKGREVVKTEASE"
     gene            1243..2082
                     /locus_tag="PLT69_08765"
     CDS             1243..2082
                     /locus_tag="PLT69_08765"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOJ14566.1"
                     /translation="MGYCLIFPGQGTQFPGMSRGLEITGAIPGTLRRLMEEGPAEELD
                     LTLNAQPAVLSVSAALWKRSGLTDPLCVMGHSLGEYTALVAAGSLDLDEAVALVTKRA
                     VLMEASSPRKGSMAAVVGLSAGEVAKVCALFEDLWVANLNGASQVVISGRAESVDAAV
                     PALKERGARKVVPLKVSVASHCPYMEQASAKLAEHLGSVGLGRPSCPVISNVTAGPET
                     DPGRIKDLLGRQLVSPVRFEESVKAACGLGVDLFVEIGPRPVLGALVRRIAPGVEVRE
                     ITA"
     gene            2222..>2879
                     /locus_tag="PLT69_08770"
     CDS             2222..>2879
                     /locus_tag="PLT69_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ14567.1"
                     /translation="MIPIDLSGQVALVTGASRGIGAAAARALGRAGAHVIVNYLSSEE
                     LAAACVEDIRTSGGNAEAARFDVSAEAEVARAFEDVMKRHGRIDILVNNAGVSKDALF
                     LRVTPEMINEMIAVNLVGPFFCTLHAARYMIKQRRGRIVTVSSIVGLGGNPGQSVYAA
                     TKAGLVGMTKSFAKELGPRGVRVNAVAPGLIDTDMTKGMDREAIAREIPLRSIGSPAE
                     V"
BASE COUNT          518 a          894 c          999 g          468 t
ORIGIN      
        1 cggcggatcg cgccccgctt ttgtgaggca gcgcgacgtg tcacgacgtg cccgggtctt
       61 cggtgggctt gtcgtgcgca cgatgaagcc cgtgtctcgc ggggtgggta tggctcgtcg
      121 tgggggacgg gggagaaggc cggccgtagc gaccgtgagg catgcggtga gggacgggag
      181 acacccaggc ggtgcccgct cgaggccgac gctggattga ggcggcaccc accgctttct
      241 cgaggcgtga tcccttcggg cgcgtgcggc ctctgccgcc gggagggggc cggaaaggcc
      301 ccccgtcccg cacgtggcgg ccgtgcgaaa ccgtccgtcg gctttccgta tcccgaaagc
      361 ggcaaggagc cctgccgctt gcggtggttt caagatagac gccgcccgcg cgccaagtcc
      421 gccggagggc ctctgaaagg gcttcttgac aaagagacct gcatgaaata gtagcatgat
      481 ctacctatgg acaggcctgt ctccatacac gacatacggc tcttggccga gaacatcggc
      541 gaggccgggc tcgagaggga tatcaccgtg tcccctgcgg acatccaggt ggtgctctcg
      601 gacgttgacg tgagcgtcga cgagcccttc tcggtccact acgaggtggt gcgccaccag
      661 gagacgttcc atgtccaggt ggacatacag ggctgcatcc acgccacctg tgcgcgctgc
      721 ctcgccccca tggaccacag ggtggatctc cacctcgaga gcgactacgt gcccgcccca
      781 agcgacatga cgggagacct cgaggcgcag cgcgtcacgg cggagacggg gttctacgac
      841 agggacatcc acctcgggaa gtacatctgt tccgagctcg tcctttctct gccctatatc
      901 tacctctgca gcgagacgtg caagggcatg tgcccgcgtt gcggggccaa cctcaacgag
      961 ggcccgtgcc tgtgcgccaa ggaagtcgac cccaggctcg aggtgctggg gagtctcaag
     1021 acaacacgct aacaccattt ccggaggaca tgagcaaatg ccactgccga agagaagaca
     1081 ctcgagcgcg agaagggaca agcgcagggc caatgacttc gccccaaccc ccgccgtctc
     1141 cttctgcccc aactgcaacg agcccaaggc gccgcacagg gtatgcccga gttgcgggta
     1201 ttacaagggc agagaggtcg tgaagacaga agcatccgag tgatgggcta ctgcctgatc
     1261 ttccccggcc agggaaccca gttcccgggc atgtccaggg ggctcgagat aacgggcgca
     1321 atccctggaa ccctgcgccg gctcatggaa gaaggccctg ccgaggagct cgacctgacg
     1381 ctgaacgcac agcctgcggt gctctccgtg agcgcggcat tgtggaagcg ctccggcctg
     1441 acggaccccc tctgcgtcat gggtcacagc ctcggcgaat acaccgcgct cgtcgccgcg
     1501 ggcagcctcg accttgatga agccgtagcc ctcgtcacca agagggctgt cctcatggag
     1561 gcctcgtccc cccgcaaggg ctccatggcc gccgtcgtgg ggctctcggc cggagaggtc
     1621 gcaaaggtgt gcgccttgtt cgaagacctg tgggtggcca atctcaacgg ggcatcccag
     1681 gtggtcatct ccgggagggc cgagtccgtc gatgccgccg tgcccgctct gaaagagcgt
     1741 ggggcgcgga aagtcgtgcc gctcaaggtc tcggtcgcct cgcattgccc gtacatggag
     1801 caggcctccg ccaagctcgc cgagcacctg gggtccgtag gcctggggag gccctcctgc
     1861 cctgtcatat cgaatgtgac agccgggccg gaaaccgacc ccggccgtat caaggacctg
     1921 ctcggccgcc agctcgtgag cccggtgcgg ttcgaggagt cggtgaaggc cgcctgcggg
     1981 ctcggggtcg acctgttcgt ggagatagga ccccggccgg ttctcggcgc cttggtgagg
     2041 aggatcgctc ccggtgtcga ggtgagggag atcaccgcct gagacacctg tggggagggc
     2101 gtcttggcgg ccgtagcgac gccgctttcc gggatgacct gaagcggcct cgtggggacc
     2161 tcgaggcagg aggacggtcc ttgcaagagg atgaacacgc acggtcaaga ggaggccgaa
     2221 gatgatcccc atcgatctgt cggggcaggt ggcgctcgtc acgggggcga gccgaggcat
     2281 tggggcggcc gctgcacgcg ccctcgggcg ggcgggcgcc cacgtgatcg tcaattacct
     2341 ctcaagcgag gagctggcgg ctgcgtgcgt cgaggacatc cgcacgtcgg gcgggaacgc
     2401 cgaggccgcc cggttcgacg tgtccgcgga ggccgaggtg gcgcgcgcct ttgaagacgt
     2461 catgaaacgg cacgggagga tcgacatcct cgtgaacaac gccggtgtct ccaaggatgc
     2521 gttgttcctg cgggtgaccc ccgaaatgat caatgaaatg atagctgtaa atctcgtggg
     2581 tccttttttc tgcactttgc acgctgcgag atatatgata aagcaaaggc gaggacggat
     2641 cgtgacggtc tcctccatcg tcggcctcgg ggggaacccg ggccagagcg tgtatgcggc
     2701 gaccaaggca gggcttgtcg gcatgaccaa gagcttcgcc aaggaactcg gaccgcgggg
     2761 ggtcagggtg aacgctgtgg cgcccggcct catcgacacg gacatgacga agggaatgga
     2821 ccgggaggcc atagcgaggg agatcccgct gcgatcgatc ggcagcccgg ccgaggtgg
//
LOCUS       DAOSCR010000353         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22405_length_2878_cov_3.737868, whole genome shotgun sequence.
ACCESSION   DAOSCR010000353 DAOSCR010000000
VERSION     DAOSCR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22405_length_2878_cov_3.737868"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLT69_08775"
     CDS             complement(<1..650)
                     /locus_tag="PLT69_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ14568.1"
                     /translation="MKHKLVRKAVREFAEAELGPIAHDIDRDARFPWEVVEKMRPLNF
                     FGLQVSRDFGGAGLDSISYAIAVEEISRISGAMGLCVTVHNSVGAYPIERFGTEDQKR
                     RFLPRLASGESIGAFCLTEANAGSDASGVETIAVDAGECFVLNGTKIFVTNGGVCSLP
                     LVFAVSDPSDPTGSSCVFVVEKTMPGFSVGEMEDLCGMRANPVSSIFLEDVRVPRE"
     gene            complement(1121..2548)
                     /locus_tag="PLT69_08780"
     CDS             complement(1121..2548)
                     /locus_tag="PLT69_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14569.1"
                     /translation="MKSRSNRGQGEGVSTGGSRGSCDDISRLKQVYLYANEFIYEHDL
                     EGNFLGISPGFIGFLGYSRDELLRMNVKDLLIEEDRRHFHHYIERVLEHKKDEGLIQI
                     LTKAGRKRIVEHKTLLLTGPGGEMSVGGIAKDVTDVLETEQALIESEMRFRMILDSIE
                     DGYFEVDLAGNFTFFNDQVAKQIGYEPDELMGMNYQQYMDRENARIVYEAFHNVFLTG
                     NPMSAVDWELRRKDGSKMYVETSVSLLRDREGKPIGFCGIIRDITERKRAEQELAYLA
                     YHDALTGLHNRKAFNEKLEEVIRDARRYEVERSVLFIDLDRFKQVNDVFGHEIGDKLL
                     MQVASRLRETLRETDYISRLGGDEFTIILGGITRSDPERVAQRVLKALSRPYRFHDII
                     IDFVTPSIGISTFPDDGHDADTIIKHADEAMYRAKQVRNSYCTYKECTKASVVEEVVE
                     ESEGRPAKPDTEERRPSLRVVVSDG"
BASE COUNT          528 a          931 c          840 g          579 t
ORIGIN      
        1 ttctcgcggg gcacccgcac gtcctccagg aagatggaag agacggggtt ggccctcatg
       61 ccgcacaggt cctccatctc gcccaccgag aacccgggca tcgtcttctc cacgacgaac
      121 acgcacgagc tgcccgtagg gtcggacggg tccgagacgg cgaagacgag ggggagcgaa
      181 cagacgcccc cgttggtgac gaagatcttc gtcccgttga gaacgaagca ttcccctgca
      241 tccaccgcga tcgtctccac gcccgaggcg tcggagccgg cgttcgcctc ggtgaggcag
      301 aacgcgccga tagactcgcc tgacgcgagc ctgggcagga accgcctctt ctggtcttcg
      361 gtcccgaagc gttcgatggg ataggcaccg acagagttgt gcacggtgac gcacaggccc
      421 atggcgccgc ttatgcggga gatctcctcc acggctatgg cgtagctgat gctgtcgagc
      481 ccggcccccc cgaagtccct ggagacctgg agaccgaaga agttcagcgg ccgcatcttc
      541 tccacgacct cccaggggaa ccgggcgtcc ctgtctatgt cgtgggcgat ggggcccagc
      601 tccgcctccg cgaactccct gaccgccttg cgtaccagct tgtgtttcat gcagggatcg
      661 agtctcacgc gcctcggccc ttcccttcct cgctcctcat tgctccggcc cgacgagcct
      721 gcggcgcacc ataacgtaaa acggcccaaa actcaaggat cccaaggttc aacgggcggt
      781 ggtaaaaaaa agggtgatgt ctgggaaact tcacccatag gggcaagtga ggaggttttg
      841 gctgctgtcg tctctgctta ttctgatgcg ctttcaggct ttctggattc ctctcgagac
      901 gggccggaaa cctggtgcgt aaaaccgcct gcttgccggt aaacgaggac gcttcccgca
      961 tctgcacaca agcccgggcg gcatcgggcc catcataagc ctggggtaaa aagccgcgtg
     1021 acgggttcgg ccggggcagg aagaaacagc ggatgacacg ccatcccgcc cgacgacacc
     1081 ctcgcaagga tgccgccagg gacacgaaca ccaaggagga tcagccgtcg gagacgacca
     1141 cccggagcga cggcctccgc tcttcggtat cgggcttggc gggtcgcccc tcgctctctt
     1201 ccacgacctc ttccacaacc gacgccttcg tgcactcctt gtaggtgcag tagctgtttc
     1261 tcacctgctt ggcacggtac atggcctcgt cggcgtgctt gatgatggta tccgcatcgt
     1321 gcccgtcgtc ggggaaggtg ctgatgccta tgctcggggt cacgaagtct atgatgatgt
     1381 cgtggaaccg gtaaggcctc gagagcgcct tcagcaccct ctgcgccacc ctctccgggt
     1441 cactgcgcgt tatcccgcca aggatgatcg tgaactcgtc acccccgagc ctgctgatat
     1501 agtccgtctc cctgagggtc tccctgagcc tggaggccac ctgcatcagc agcttgtccc
     1561 ctatctcgtg gccgaacacg tcgttgacct gcttgaacct gtcgaggtcg atgaacagga
     1621 ccgaccgctc cacctcgtag cgcctcgcgt cccgtatgac ctcttccagc ttctcgttga
     1681 acgccttcct gttgtggaga cccgtgagtg cgtcgtggta tgcgaggtaa gcgagttcct
     1741 gctcggcgcg cttgcgttcc gttatgtcgc ggatgatgcc gcagaacccg atgggcttgc
     1801 cttccctgtc ccgcaggagg gagaccgagg tctccacgta catcttcgag ccgtccttgc
     1861 gcctgagctc ccagtccacc gcggacatgg ggttgcctgt caggaagacg ttatggaagg
     1921 cctcgtagac gatcctcgcg ttttccctgt ccatgtactg ctggtagttc atgcccatga
     1981 gctcgtcggg ctcgtacccg atctgcttgg ccacctggtc gttgaagaag gtgaagttcc
     2041 ccgcgaggtc cacctcgaag tacccgtcct cgatggagtc gaggatcatc ctgaaccgca
     2101 tctcgctctc gatgagggcc tgctcggtct cgaggacgtc cgtcacgtcc ttggcgatcc
     2161 cgcctacgct catctcgccg cccggcccgg tcaacaggag ggtcttgtgc tcgacgatgc
     2221 gcttgcggcc ggccttcgtg aggatctgta tcaggccctc gtccttcttg tgttccaaca
     2281 cgcgttcgat atagtgatgg aagtgcctcc tgtcctcctc gatgagcagg tccttgacgt
     2341 tcatgcgcag gagttcatcc ctgctgtagc ccagaaagcc gatgaatccc gggcttatcc
     2401 ccaggaagtt gccctcgaga tcgtgctcgt agatgaactc gttcgcgtac aggtagacct
     2461 gcttgaggcg ggagatatcg tcgcaggagc cccgggagcc accggtggag acaccttctc
     2521 cctgccctct gttcgagcgt gatttcattc tgctcatcgt accttcatgc caaccgggca
     2581 gatcaattta atgaaatatc ggataaagac atggcagaca ttaacatgac gggaagaaag
     2641 accccggaca ggaccttgaa gtgcacccat ccatgccatg tgccttccgg acttcagcac
     2701 ttcatgacga ccctctctca gccccgatca gggcctccaa gcccgcccgg gcaggagcgg
     2761 aagccaagcg cccgttccga cggggcatga cgctcaggcc catgcggaca cgccgcatgt
     2821 ggatgaagtg acaggccctg ggcgtcaagt tcagaaagac cgtgctcagg ccaccgga
//
LOCUS       DAOSCR010000354         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22446_length_2875_cov_3.518085, whole genome shotgun sequence.
ACCESSION   DAOSCR010000354 DAOSCR010000000
VERSION     DAOSCR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22446_length_2875_cov_3.518085"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1828)
                     /gene="flgK"
                     /locus_tag="PLT69_08785"
     CDS             complement(56..1828)
                     /gene="flgK"
                     /locus_tag="PLT69_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOJ14570.1"
                     /translation="MPGLGGALDIARWSMYSSQLAMEIVSHNVANANTEGYSRQSLRV
                     EAGYPITMGPGQIGTGVRAVEVLRNYDNFLNQQVTLKKSEYSFWSASRSAMEEIESIF
                     NETDGYGLNYLMGEFWNAWGDLSNNPDGVPERQALVNKTENLLSMIHEIDYNLRSYQR
                     HLDGNIRGSVTEVNSIIRQIADLNKSISSVEIDGMINANDLRDRRELLLEQLSQYMDI
                     TYYEEESTGQVQVYIMGGTPLVLGYDTYEFVTSRNDATGFTDIFWQDSSGRQVNVTDK
                     LRAGKIAGWVNVRDNTIGSYIDSLNTMVGELVWRLNEIHSQGVGLAGVTSMESTVGGM
                     TAASDLSSDFLFSDRYVAGAFEIIVYDSSGQVAGSYHIDPAGTTIGDLVNEINAESGV
                     GGPITASITAEGRFRIDAGTGYTFAVAPAATGTNGNALAVMGVNTFFSWNETVGSPLD
                     DITETIGLNDAVAQDPTRISTGRPDASGLVAPGSNVTARQIYSLQDLVIPDIGGTGVS
                     TTLDAFYSSLVAQVGVDVQNSINNEKFNDTLLGQYLRRKESVTGVNIDDEMAELIKLQ
                     HLYQASAKLISVADEMMEALISIK"
     gene            complement(1830..2252)
                     /gene="flgN"
                     /locus_tag="PLT69_08790"
     CDS             complement(1830..2252)
                     /gene="flgN"
                     /locus_tag="PLT69_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOJ14571.1"
                     /translation="MTPSELIQRYERECEMVGALVEHLRRELELISSDDVQGLEDSMP
                     AKYRILGEIQKLRQDAAVPEVEPTAAEASRLRALQQRLVMSWREASGLNEIAKDMVTK
                     RLKDLDRAVQSCLSALKDGYAKDGKRHGVSHHTLRTGA"
     gene            complement(2249..2620)
                     /locus_tag="PLT69_08795"
     CDS             complement(2249..2620)
                     /locus_tag="PLT69_08795"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOJ14572.1"
                     /translation="MIDDVSMIAAMNRTDPAKALKRACAEFESLFAYQLLKAMYETVP
                     EGFMEEGLAGDVYRDMFSFEVARRIGETGALGIARVLEQTLRERFGYEDASSARGPEG
                     KEADKTDMHRGEAKITAGGQT"
     gene            complement(2617..>2875)
                     /locus_tag="PLT69_08800"
     CDS             complement(2617..>2875)
                     /locus_tag="PLT69_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOJ14573.1"
                     /translation="LRVKVTEQPQVSQPLPRSRGVTTVVPQSGVEVEEGGKNLIMLRG
                     GATIAELVNALNAVGVSPRDLVVILQSIKRSGALYADLEVI"
BASE COUNT          444 a          965 c          839 g          627 t
ORIGIN      
        1 agtatcagca atgacgcccc ccgatggatg agtgctcagg ggaatgtgta catgattatt
       61 ttatcgaaat caacgcttcc atcatctcgt cggcgacgga tatgagcttg gccgaggcct
      121 ggtagagatg ctgcagcttg atgagctcgg ccatctcgtc gtcgatgttc acgcccgtga
      181 cgctctcttt tctcctcagg tattgcccga ggagggtgtc gttgaacttc tcgttgttga
      241 tgctgttctg cacgtccacg ccgacctgcg cgacaagcga gctgtagaac gcgtcgagcg
      301 tcgtgctcac ccccgtgccg cctatgtccg gtatgaccag gtcctggagg gaatagatct
      361 gcctggccgt cacgttcgaa ccgggagcca cgaggcctga ggcgtccggc cgccccgtgg
      421 atatcctcgt gggatcctgg gccactgcgt cgttcagccc gatcgtctcg gtgatgtcgt
      481 ccaggggcga tcccacggtc tcgttccagg aaaagaacgt gttcacgccc atgacggcaa
      541 gggcgttgcc gttcgtaccg gtggccgccg gggcgaccgc gaaggtgtag ccggtccccg
      601 catcgatcct gaacctgccc tccgccgtga tgctcgcggt gataggcccg ccgacgcccg
      661 actcggcgtt gatctcgttc accaggtcgc cgatcgtggt ccccgcaggg tcgatgtggt
      721 acgatcccgc cacctgcccc gacgagtcgt acacgatgat ctcgaacgcg cccgcgacgt
      781 agcggtcgct gaagaggaag tcactcgaga ggtcggaggc cgcggtcatc ccgccgaccg
      841 tgctctccat cgacgtgacc ccggccaacc ccacaccctg ggagtggatc tcgttcaagc
      901 gccagacgag ctcacccacc atggtgttca ggctgtcgat gtacgagccg atggtgttgt
      961 ccctgacgtt cacccacccg gctatcttgc ccgccctgag cttgtccgtc acgttgacct
     1021 gcctcccgga ggagtcctgc cagaagatgt ccgtgaaccc cgtggcgtcg ttgcgcgagg
     1081 tgacgaactc atacgtgtcg tatcccagga cgagcggcgt gcctcccatg atgtacacct
     1141 ggacctggcc cgtgctctcc tcctcgtagt aggtaatgtc catgtactgg gagagctgct
     1201 ccaggaggag ttccctgcgg tcgcgcaggt cgttggcgtt gatcatgccg tcgatctcca
     1261 cgctcgagat gctcttgttg aggtccgcga tctgcctgat gatggagttc acctctgtca
     1321 cggagcccct gatgttcccg tcgaggtggc gctggtagga tctcaggttg tagtcgatct
     1381 cgtggatcat ggagagcagg ttttccgtct tgttcacgag cgcctgcctc tcgggcacac
     1441 cgtcggggtt gttcgacagg tcaccccatg cgttccagaa ctcgcccatg aggtagttga
     1501 ggccataccc gtccgtctcg ttgaagatgc tctcgatctc ctccatggcg ctcctgctcg
     1561 cgctccagaa cgagtactcg gacttcttga gggtcacctg ctggttgagg aaattgtcgt
     1621 agttcctcag cacctcgacg gccctcacgc cagtgccgat ctgtcccggc cccatggtga
     1681 tggggtaacc ggcttccacg cgcaggctct gcctcgagta accctcggtg ttcgcgttgg
     1741 ccacgttgtg ggagacgatc tccatggcca gctgcgagct gtacatggac caccgtgcga
     1801 tgtcgagcgc tccaccaagt cccggcatgt catgcccccg tacgcagggt gtggtgactc
     1861 accccgtgtc tcttcccatc cttggcgtac ccgtccttga gtgccgagag gcacgactgg
     1921 accgccctgt cgaggtcctt gagcctcttc gtcaccatgt ccttcgctat ctcgttgagc
     1981 cccgaggcct ccctccacga catcacgagc ctctgctgca aggccctgag cctcgacgcc
     2041 tcggccgccg tcggctccac ctccggtacc gcggcatcct gcctgagttt ctggatctcc
     2101 ccgaggatcc tgtacttcgc gggcatggaa tcctcgagcc cctgcacgtc gtcggagctg
     2161 atgagctcga gttcccggcg caaatgctcg accagcgccc ctaccatctc gcactcccgc
     2221 tcgtacctct ggatgagttc cgacggtgtc atgtctggcc tcctgccgta atcttggctt
     2281 ctcctcggtg catgtccgtc ttatcggctt ctttcccttc gggaccccta gcgctcgagg
     2341 cgtcctcgta cccgaagcgc tccctcaggg tctgctcgag gaccctggct atccccaagg
     2401 cgcccgtttc cccgatcctt ctcgcgacct cgaaggagaa catgtcccgg tatacgtcgc
     2461 ctgccagccc ctcttccatg aacccctcag gcaccgtctc gtacatcgcc ttgaggagct
     2521 ggtacgcgaa gagggactcg aactcggcgc acgccctttt cagggccttg gcggggtcgg
     2581 tcctgttcat ggccgcgatc atggacacgt catcgatcat atgacctcca ggtcggcgta
     2641 gagcgcccct gaccgcttga tgctctggag gatcaccacg aggtcccgcg gcgatacccc
     2701 gaccgcgttc agggcgttca cgagctccgc tatggtggcc ccgcccctga gcatgatgag
     2761 gttcttgccg ccttcctcga cctcgacccc gctctggggc accacggtgg tgacccccct
     2821 cgacctgggc aggggctggg agacctgggg ttgctccgtg accttcacgc gcagg
//
LOCUS       DAOSCR010000355         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22454_length_2874_cov_3.770486, whole genome shotgun sequence.
ACCESSION   DAOSCR010000355 DAOSCR010000000
VERSION     DAOSCR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22454_length_2874_cov_3.770486"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLT69_08805"
     CDS             <1..947
                     /locus_tag="PLT69_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ14574.1"
                     /translation="SPNHKDRPFDKGEVAYWMPVDQYIGGIEHAILHLMYARFYTMVL
                     KDLGYVDFEEPFLRLLTQGMVIKDGAKMSKSKGNVVDPEDMIQRYGADATRLFTLFAA
                     PPEKDMDWTDQGIEGTFRFLSRLWRLVMEIKDMPGTPSASSVPASMAPLMQKTHQTIK
                     KVTDDIEKRFRFNTAIAAMMELVNDLYRFKEKAGSGEEISVLKHSVEQLLVMLSPFVP
                     HIADELWEAIGNQGMLFDHPWPKEDPAWAKSKTSTVAVQVSGKLRATIEVPEGSPKEA
                     VEQRALEEQNVRRFTEGMKVVKVIYVPDKIINFIVAPS"
     gene            997..1377
                     /gene="lptE"
                     /locus_tag="PLT69_08810"
     CDS             997..1377
                     /gene="lptE"
                     /locus_tag="PLT69_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOJ14575.1"
                     /translation="MSVDLAPSANTTQLVEAGIALDRQLELSLRQLGLLSNPHADRVL
                     RCTVVEARTSRISTASLAATDRYRLALTVNASLMTRGGSVLWERTFTDWGTYSEGQAA
                     EAALHETCARISRQVASSIASLSP"
     gene            1374..2351
                     /gene="holA"
                     /locus_tag="PLT69_08815"
     CDS             1374..2351
                     /gene="holA"
                     /locus_tag="PLT69_08815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ14576.1"
                     /translation="MKKKPTSSGRGPNYVFVGIEEFLLEEELSRIKQSIDGDELMNWN
                     VFNADEPSRINADEIVALCNTLPFLASRRAVVIRNAQNLKADQAGRIASYMDDPCDAT
                     TLVLCLDMVDSERGVEGVLRAFKGRADIVRFEPMKDKNERIRWILERARGLGKTMERD
                     AAAMLAETAGTSLWQLKGEIEKLCLYVGHNPTVTIQDVREVAMGSVEPAVFAFLDSLV
                     ERRKDAIVRLGDLEGCGVSDLELTGRLENEIIVHYVVLTGQDARKLKIHDFRIQKASR
                     RKHHWNGDELLELLGETRRIEQGIKSSASTGGYGALAELVGRVVLGHRS"
     gene            complement(2389..2655)
                     /gene="rpsT"
                     /locus_tag="PLT69_08820"
     CDS             complement(2389..2655)
                     /gene="rpsT"
                     /locus_tag="PLT69_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ14577.1"
                     /translation="MANHPSALKRQRQSRKRRMRNRSYKSAINTTIKKIFASLGEKDT
                     DKAGAFAKEAVSRLDSAVTKGILHRRTASRKISRLMKKIGAQLA"
BASE COUNT          571 a          906 c          906 g          491 t
ORIGIN      
        1 gcagccccaa ccacaaggac aggcccttcg acaaaggcga ggtggcctac tggatgcccg
       61 ttgaccagta catagggggc atcgagcacg cgatcctgca cctcatgtac gccaggttct
      121 acaccatggt cctcaaggac ctcggttacg tcgatttcga ggagccgttc ctgcgcctgc
      181 tgacgcaggg catggtcatc aaggacgggg ccaagatgtc gaagtccaag ggcaacgtgg
      241 tggaccccga ggacatgata caacgctacg gcgcggacgc cacgaggctc ttcaccctgt
      301 tcgccgcccc gcccgagaag gacatggact ggaccgacca gggcatcgag gggaccttca
      361 ggttcctctc gaggctctgg aggctcgtga tggagatcaa ggacatgccc ggcactccct
      421 cggcctcgtc ggtgcccgca tccatggccc ccctcatgca gaagacgcac cagacgatca
      481 agaaggtcac cgacgacata gagaagcggt tcaggttcaa caccgcgatc gcggccatga
      541 tggaactcgt caacgacctg taccggttca aggagaaggc gggctcgggt gaggagatct
      601 ccgtgctcaa gcactcggtg gagcagctct tggtcatgct ctcccccttc gtcccccaca
      661 tcgccgacga gctctgggaa gccatcggca accagggcat gctcttcgac cacccctggc
      721 cgaaggaaga ccccgcatgg gcgaagtcga agacctcgac ggtcgcggtg caggtctccg
      781 gaaagctcag ggcgaccatc gaggtccccg agggctcacc caaggaagcg gtggagcaga
      841 gggccctgga ggagcagaac gtcaggcgct tcaccgaggg catgaaggtc gtgaaggtca
      901 tctatgtccc ggacaagatc attaatttca ttgttgctcc ttcttgagct ggccgggtgc
      961 gcctatagct tctctcccgg ggtgaaccag gcgaccatgt ccgtcgacct ggccccaagc
     1021 gccaacacaa cccagctcgt cgaggcgggc atagccctcg acaggcagct cgagctctcc
     1081 ctcaggcagc tcggactcct gtccaacccc catgccgacc gcgtcctccg atgcaccgtc
     1141 gtggaggcac ggacatcgag gatatcaacg gcatcgctcg cagcgaccga ccgataccgt
     1201 ctcgccctca cggtcaacgc ctccctcatg accaggggcg ggtccgtcct ctgggaaagg
     1261 accttcacgg actggggcac gtactccgag ggccaggccg cggaggcggc cctccatgaa
     1321 acgtgcgcca ggatatccag gcaggtcgca agctccattg cctccctgag cccatgaaga
     1381 agaagccgac ctcgtccggg cggggcccga actacgtgtt cgtgggcatc gaggagttcc
     1441 tcctcgagga ggagctctcg aggatcaagc agtccataga cggcgacgag ctcatgaact
     1501 ggaacgtgtt caacgccgac gagccttcga ggatcaatgc cgacgagatc gtggcactgt
     1561 gcaacaccct ccccttcctc gcatcgagac gcgcggtggt gatccgcaac gcgcagaacc
     1621 tcaaggcgga ccaggcggga aggatcgcct cgtacatgga cgacccgtgc gacgccacga
     1681 ccctcgtttt gtgcctcgac atggtggatt ccgagcgcgg cgtggagggt gtactgaggg
     1741 cgttcaaagg cagggcggac atcgtgaggt tcgagcccat gaaggacaag aacgagcgca
     1801 tacggtggat cctcgagagg gcgaggggcc tgggcaagac catggagagg gatgccgcgg
     1861 cgatgttggc cgaaacggca ggcacctccc tctggcagct caagggcgag atagagaaac
     1921 tctgcctgta cgtgggtcac aatcccacgg tcaccataca ggacgtcagg gaggtcgcca
     1981 tgggctcggt ggaacccgcg gtcttcgcct tcctggacag cctggtcgaa cgcaggaaag
     2041 acgccatcgt gcgcctgggg gatctcgagg gctgcggggt gtcggatctc gagctcacgg
     2101 gaaggctcga aaacgagatc atcgtgcact acgtggtgct caccggccag gacgcccgca
     2161 agctgaagat ccacgatttc aggatccaga aggcgtcgcg caggaaacac cactggaacg
     2221 gcgacgaact cctcgagctc ctcggggaga cgagacgtat cgagcagggc atcaagtcga
     2281 gcgcgtcaac gggcggttac ggcgccctcg cggaactcgt gggaagggtg gttctcgggc
     2341 accggtcctg agaaaccggt caaggggcaa ggcggtccgt gcgcccgctc acgcgagctg
     2401 ggcgccgatc ttcttcatga gcctggagat cttcctggag gccgtcctcc ggtggagaat
     2461 gcccttggtc accgcgctgt ccagcctgga gaccgcttcc ttggcgaacg caccggcctt
     2521 gtcggtatct ttctcgccga gcgaggcgaa tatcttcttg atggtggtgt tgatggcgga
     2581 cttgtagctc ctgttcctca tccgccgctt acggctctgt cgctgcctct tcaaggccga
     2641 cgggtgattg gccatggacg cgaatccctc cttctttcgt gaatccgagg tgtttcttct
     2701 atccgctcga tctctgcgtg tcaagcgttt tcctgtcggc ggccgaaggg tgtgccgaga
     2761 acgggcccaa gcacccggtc cttgccagag ccgcgcatct tcccaaggac acgctcagga
     2821 cctcggcctg cccgaagccg cgtgtgaccc accgccatgc gccagaccga cgtg
//
LOCUS       DAOSCR010000356         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22476_length_2872_cov_2.888179, whole genome shotgun sequence.
ACCESSION   DAOSCR010000356 DAOSCR010000000
VERSION     DAOSCR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22476_length_2872_cov_2.888179"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="glmM"
                     /locus_tag="PLT69_08825"
     CDS             <1..1176
                     /gene="glmM"
                     /locus_tag="PLT69_08825"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ14578.1"
                     /translation="SAIAAGVCSMGADALLAGVMTTPGVAFLTTSMRADAGIVISASH
                     NPYMDNGIKIFSKDGFKIADAMEEELERLVLSGDPLPHPSPSLRVGLASRIDDAPGRY
                     IVFIKKSFPEEYSLEGMKVVLDCANGAAYRIAPEVFSELGAKVVTISHDPDGTNINEG
                     CGSQHPEALAEEVVKNGADAGFAFDGDGDRVIAVDEQGSVLTGDHILALCALSMKRRG
                     ELRNNLLVTTVMANLGLKVAMRDHGVDLSFTDVGDRSVLEEMLKKGARIGGEQSGHII
                     FLDHHTSGDGIISALQVLRVLREEKRPLSQLGKVMRVFPQRLINVEVKSKPDLDTIPE
                     ITSVVSQVERLLGEDGRVLVRYSGTQRMCRVMVEGPTEEETEEYCRRIARVIREKIG"
     gene            1186..2418
                     /locus_tag="PLT69_08830"
     CDS             1186..2418
                     /locus_tag="PLT69_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14579.1"
                     /translation="MDIVRALLDKGVRIPHPASVFIAADVDPSRISGDGVTIHPGCRI
                     TGPETLVMEGAELGRETPLTVENCQVGPNVSLKGGYVSRSVFLAHSSMGSGAHVREAC
                     ILEEHASCAHSVGLKHTILFPFVTLGSLVNFCDCLMAGGTGRKDHSEVGSSYIHFNFT
                     PRQDKATASLIGDVPHGVMLDRKPIFLGGQGGLVGPSSISYGTTIAAGVVFKGRLEEE
                     CVFVGRGEGGPALKTGATGGVKDKVVLNVTYIANLVALRAWYAHVRTLFAPAGTMARW
                     LTAGAVDKLELAMDERISRLEGFVDKLRETGVTRGRATGGTSRGDPALPGELVSNKDR
                     LTDRIMSMRSYEGDPRLRDAFLDTLRRAGGVSGRDYLETIKGLGGEARSMGTAWLSEV
                     VTKVVSAVLEVIPAFRAG"
     gene            complement(2547..>2872)
                     /locus_tag="PLT69_08835"
     CDS             complement(2547..>2872)
                     /locus_tag="PLT69_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ14580.1"
                     /translation="GAKEVHMRVACPTLIYPCDFLNFSTSRSQLDLAGRKAILALEGR
                     DDMHLEEYASTGSDRNRAMTEWIRKTLGMTTLKYQRLEDLVDAIGLPKEKLCTHCWDG
                     SSYGG"
BASE COUNT          519 a          880 c         1020 g          453 t
ORIGIN      
        1 agcgcgatcg cggccggggt gtgctcgatg ggggcggacg cgctgctggc cggtgtcatg
       61 accacgccgg gggtggcctt cctcacgacg agcatgcgtg cggacgcggg gatcgtgatc
      121 tcggcttccc acaacccgta catggacaac ggcatcaaga tcttctcgaa ggacgggttc
      181 aagatcgccg acgccatgga agaggagctg gagaggctcg tcctgtcagg ggatcccctg
      241 ccgcacccgt ccccttccct gagggtgggg ctcgcctccc gtatcgacga cgccccgggc
      301 cgatacatcg tcttcatcaa gaagtctttc cccgaggagt actcgctgga gggcatgaag
      361 gtggtgctcg actgtgccaa cggcgcggcg taccgtatag cgcccgaggt cttctcggag
      421 ctcggcgcaa aggtggtcac gatctcccat gacccggacg gcacgaacat caacgagggg
      481 tgcgggtccc aacaccccga ggcgctcgcc gaggaggtcg tgaagaacgg ggcggacgca
      541 gggttcgcct tcgacgggga cggggaccgc gtcatagcgg tggacgagca gggctcagtc
      601 ctcacgggcg accacatact cgccctgtgc gccctgtcca tgaagaggag gggggaactg
      661 cgaaacaacc tgctcgtcac caccgtcatg gccaacctcg gcctcaaggt ggccatgcgg
      721 gatcacgggg tggacttgag cttcacggat gtcggcgacc gttcggtcct ggaggagatg
      781 ctcaaaaagg gggcgaggat cggtggcgag cagtccgggc acatcatctt tctcgaccac
      841 cacacctcgg gcgacggcat catatccgcc ctccaggtgc tcagggtcct cagggaggag
      901 aagaggcccc tgtcccagct gggcaaggtc atgagggtgt tcccgcagag gctcatcaac
      961 gtggaggtca agagcaagcc cgacctcgac accatacccg agatcacctc tgtcgtctcc
     1021 caggtggaga ggctgctcgg cgaggacggc cgggtgctcg tgaggtactc cggcacacag
     1081 aggatgtgcc gcgtcatggt cgaggggccc accgaggagg agacggagga gtactgcagg
     1141 cgcatagccc gggtgatccg ggaaaagatc ggttgaggag ggtcgatgga catcgttcgg
     1201 gcactcctgg acaagggggt gaggatccct catcccgcga gcgtcttcat agcggcagat
     1261 gtcgatcctt cgaggatctc gggtgacggc gtgaccatac accccggctg caggatcacc
     1321 gggcccgaga ccctcgtgat ggagggcgcg gaactcggga gagagacccc cctcaccgtg
     1381 gagaactgcc aggtcggccc caacgtcagc ctcaagggcg gctacgtcag ccgctcggtg
     1441 ttcttggccc actcgtccat gggatccggg gcgcacgtgc gggaggcctg catcctcgag
     1501 gagcacgcca gctgcgccca cagcgtcggc ctcaagcaca cgatcctctt ccccttcgtc
     1561 acgctcggca gcctcgtcaa cttctgcgac tgcctcatgg caggggggac gggcaggaag
     1621 gaccacagcg aggtggggag ctcgtacatc cacttcaact tcaccccgcg ccaggacaag
     1681 gcgaccgcat cgctcatcgg tgacgtgccg cacggggtca tgctcgacag gaagcccata
     1741 ttcctgggcg ggcagggcgg gctggtgggc ccctcctcga tctcctacgg gaccactatc
     1801 gccgcgggcg tcgtgttcaa gggcaggctc gaggaagagt gcgtgttcgt gggaagaggc
     1861 gaaggcggtc ccgctctgaa gaccggggcc accggcggcg tcaaggacaa ggtcgtcctc
     1921 aacgtcacct acattgcgaa cctcgtcgcg ctccgcgcat ggtacgcgca cgtgaggacc
     1981 ctgttcgcgc ctgcaggcac catggcccgg tggctcacgg ccggggccgt ggacaagctg
     2041 gagctggcca tggacgagcg gatatcgagg ctggaagggt ttgtcgacaa gctcagggaa
     2101 acaggggtca cccggggccg agccacggga gggacctcgc ggggggaccc cgccctcccg
     2161 ggggaactcg tctccaacaa ggacaggctt acggacagga tcatgtccat gaggtcctac
     2221 gagggggacc cgaggctgag agacgccttc ctcgacacgc tccgccgggc gggcggggtt
     2281 tcgggccggg actacctgga gacgatcaag ggcctagggg gggaggccag gtcgatgggg
     2341 acggcgtggc tgagcgaggt ggtgacgaag gtcgtgtcgg cagtcctcga ggtcatcccc
     2401 gccttcaggg ccgggtgagc ccggcgagca ggaagcaggc gcctccccat ggcgatgtcg
     2461 cggctgcacc gggcagaccc cgccccgatg gcgcctgcat acccaagggg gcagggggtg
     2521 aaggccgggg tgagccgggc aggggcttac ccgccgtagc tcgacccatc ccagcaatgg
     2581 gtacagagct tctccttggg gaggccgatg gcgtcgacga gatcttccag cctctggtac
     2641 ttgagggtgg tcatgccgag cgtcttcctg atccactcgg tcatggcgcg gttacggtcg
     2701 gaccctgtcg atgcatactc ctcgaggtgc atgtcgtcgc ggccctcgag ggcgaggatg
     2761 gccttgcggc cggccaggtc gagctgggaa cgggacgtgg aaaagttcag gaagtcgcag
     2821 ggatagatga gggtagggca tgccacgcgc atgtgcacct ctttcgcgcc gt
//
LOCUS       DAOSCR010000357         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22531_length_2867_cov_3.061166, whole genome shotgun sequence.
ACCESSION   DAOSCR010000357 DAOSCR010000000
VERSION     DAOSCR010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22531_length_2867_cov_3.061166"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLT69_08840"
     CDS             complement(<1..323)
                     /locus_tag="PLT69_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14581.1"
                     /translation="MRFDLKIAGRDVCVRLERLDDGFVAAWVDDRRIEASFAAVTKNV
                     QYLTAGPGSQKVCLERTPEGTYVNVKGATWLVRDAHAADAGRPRRKGGAPLQREVTPP
                     MPSVVV"
     gene            complement(541..936)
                     /locus_tag="PLT69_08845"
     CDS             complement(541..936)
                     /locus_tag="PLT69_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14582.1"
                     /translation="MRVYSCLYPGCTVPMEYDPILAKLVVHAETRDACIQRAMRALRE
                     FAVMGVLTPIPFLADVIASEAFRRGETTTDFVDDFIEGWTPDVSGADIVALAAAIHLS
                     LSRERTVDGHVVHTPAEYSPWLSLKGFRP"
     gene            complement(952..2031)
                     /locus_tag="PLT69_08850"
     CDS             complement(952..2031)
                     /locus_tag="PLT69_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOJ14583.1"
                     /translation="MFDTILVANRGEIAVRIMATCREMGIRTVGVYSDVDRHALHVIE
                     ADRAVALGGSNPAETYLDIDKIIAAARAEGAQAIHPGYGFLAENAQFARRCEQEGIVF
                     IGPPSDVIRDLGDKTLARRVMHAGGVPVIPGMMEPSSDIRELVQKAEEIGFPVMVKSA
                     KGGGGKGMRVVETPQACAEACEAASRESMNAFGSADIYVEKYLRSPRHVEVQVLADAS
                     GKVLHLYERECSIQRRHQKIIEETPSPSLTPPVRRSICEAALAAARAAGYVNAGTVEF
                     LLDEEGGFYFLEVNTRLQVEHPVTEMVTGVDIVRRQIEIAAGMPLDLDQDDIVQRGHA
                     IECRIYAEDPMNSFMPCPGKVVFVQ"
     gene            complement(2072..>2867)
                     /locus_tag="PLT69_08855"
     CDS             complement(2072..>2867)
                     /locus_tag="PLT69_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ14584.1"
                     /translation="AMREPRPPVYDPKEIYGIVSRDLRVPYDCREIIARMVDGSEFLE
                     FKELYGTTLVCGFASIHGYPVGILGNNGVLFSDSSLKATQFIQLCDRRGIPLVFLQNI
                     NGYIIGREYERLGITKDGHKMVNAVSTCTVPKFTVIVGASFGAGNYGMCGRAYSPRFL
                     WMWPNAQIGVMGGEQAAGVLITLKNDQNAKAGLPPLTPEEEEAIRGPIVEAASQQGDA
                     YYSTANLWDDGILDPAQTRDVLGLAISAALNAPLRDDAVGFGIFRM"
BASE COUNT          424 a         1024 c          914 g          505 t
ORIGIN      
        1 accaccaccg agggcatggg cggcgtgacc tccctctgga ggggggcgcc ccccttcctt
       61 ctgggcctcc cggcatcggc ggcgtgagcg tccctgacga gccacgtcgc gcccttcacg
      121 ttcacgtacg tgccctcggg ggtcctctcc aggcacacct tctgtgaccc ggggccggcc
      181 gtcaggtact gcacgttctt cgtcacagcg gcgaaggagg cctcgatgcg cctgtcatcc
      241 acccaggcgg cgacgaaacc gtcgtcgagc ctctccagcc tcacgcacac gtccctgccc
      301 gctatcttca ggtcgaacct cacgtgcatc ctcctccttc cggatcgtgg acggccgcgc
      361 aggcgagcga tccgaccgcc tccacgccgg gccccctgtg catccaccac cccgtcccgc
      421 tatccgtgcg gcaggcgcgg acgggggccc agccgcccta gcccgggagc ggcgccgggg
      481 gcacgacgcc gcgtgagggc acccgcatgc ggccgggagc ggtcatgtcc tgagatccac
      541 tcacggcctg aaccccttga gtgacagcca gggggagtac tcggcggggg tgtgcacgac
      601 atggccgtcc accgtgcgtt cccgcgagag ggacaggtgg atggccgcgg ccagggcgac
      661 gatgtcggcc ccgctcacgt ccggggtcca tccctcgatg aagtcgtcga cgaagtcggt
      721 ggtcgtctcg cccctcctga acgcctcgct cgcgatcacg tccgccagga aggggatggg
      781 ggtgagcacg cccatgaccg cgaattccct gagtgcccgc atagccctct ggatgcaagc
      841 gtcccgcgtc tcggcgtgaa cgacgagctt cgcgaggatc gggtcgtatt ccatgggcac
      901 cgtgcacccg ggatagaggc aggagtacac cctgatgccg gggcctttcg gctactgcac
      961 gaagaccacc ttgccggggc acggcatgaa ggagttcatg gggtcctcgg cgtagatgcg
     1021 gcactcgatc gcgtgaccgc gctgcacgat gtcatcctgg tcgaggtcga gcggcatgcc
     1081 tgcggcgatc tcgatctgcc tcctgacgat gtcgacgccc gtgaccatct cggtgacggg
     1141 gtgctcgacc tggagcctcg tgttcacctc gaggaagtag aaaccgcctt cctcgtcgag
     1201 gaggaactcc acggtccccg cgttcacgta gccggcggcc cgggcggccg caagcgccgc
     1261 ttcgcagatg gagcgcctca ccggcggggt caacgacggc gacggggtct cctcgatgat
     1321 cttctggtgc ctgcgctgga tggagcactc gcgctcgtag aggtgcagca ccttgcccga
     1381 ggcgtccgcg agcacctgca cctccacgtg cctgggcgac ctgaggtact tctcgacgta
     1441 gatatccgca ctgccgaacg cgttcatgga ctccctggat gcggcctcgc acgcctcggc
     1501 gcacgcctgg ggggtctcca cgacgcgcat gcccttgccc cctcccccct tggcggactt
     1561 gaccatcacc gggaaaccga tctcttcggc cttctgcacg agctcgcgta tgtcggaaga
     1621 cggctccatc atccccggta tcacgggaac gcccccggcg tgcatgaccc tgcgtgcgag
     1681 ggtcttgtcg ccgaggtcgc ggatgacgtc gctcgggggg ccgatgaaca cgataccctc
     1741 ctgttcgcag cgcctcgcga actgtgcgtt ctcggccagg aaaccgtagc cggggtgtat
     1801 cgcctgggcg ccctcggcgc gcgcggccgc gatgatcttg tcgatgtcga ggtaggtctc
     1861 ggcggggttc gacccgccga gcgccaccgc cctgtcggcc tcgatcacgt ggagcgcgtg
     1921 cctgtccacg tccgagtaca cccccacggt gcgtatgccc atctcccggc atgtcgccat
     1981 gatgcgcacc gcgatctccc cccggttcgc gacgaggatg gtgtcgaaca cgatcgtccc
     2041 ccccttcttc cctcacccgg tcccctgggc cctacatccg gaagattccg aagcccacgg
     2101 cgtcgtcccg aagaggcgcg ttcagtgcgg ccgatatggc caggccgagc acgtccctgg
     2161 tctgcgccgg gtcgaggatg ccgtcgtccc agaggttggc ggtgctgtag taggcgtccc
     2221 cctgctgact cgcggcctcc acgatgggtc cccggatcgc ctcctcttcc tcgggtgtca
     2281 agggcggcag gcccgccttc gcgttctggt cgttcttgag ggtgatgagc acgcccgcag
     2341 cctgctcgcc ccccatgacc ccgatctggg cgttcggcca catccacaag aatctcgggg
     2401 agtacgccct cccgcacatc ccgtagttgc cggcgccgaa ggaggcgccc acgatgaccg
     2461 tgaacttcgg gaccgtgcac gtcgagacgg cgttgaccat cttgtgcccg tccttggtga
     2521 tgccgagcct ctcgtactcg cggccgatga tgtacccgtt gatgttctgc aggaacacga
     2581 gggggatgcc tctccggtcg cacagctgga tgaactgcgt cgccttcagc gagctgtccg
     2641 agaacagcac cccgttgttg ccgaggatcc ccaccgggta gccgtggatg gaggcgaacc
     2701 cgcacacgag cgtcgtgccg tagagttcct tgaactccag gaactcgctg ccgtccacca
     2761 tcctcgcgat gatctccctg cagtcgtacg ggaccctcaa gtccctgctc acgatgccgt
     2821 agatctcctt cgggtcgtag accggcggcc tgggctcccg catcgcc
//
LOCUS       DAOSCR010000358         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22607_length_2859_cov_4.194009, whole genome shotgun sequence.
ACCESSION   DAOSCR010000358 DAOSCR010000000
VERSION     DAOSCR010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22607_length_2859_cov_4.194009"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLT69_08860"
     CDS             <1..522
                     /locus_tag="PLT69_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14585.1"
                     /translation="WAAGAQWIPPSASKDGLGTGRHQLVPTLGARYDLGGWMQGAWCG
                     ARLGHAFDVAGYEGYPHISQTVFQPMLNIDLPGSWFLTFSPEARYDWKNEEWFIPFDI
                     TAGKLAQNGLVVLFTYRSAINDDMLLYVNTFELRVRYLFDADVRTILSGASSAVNDVL
                     GGTPMDIPEPKGP"
     gene            complement(1409..2815)
                     /locus_tag="PLT69_08865"
     CDS             complement(1409..2815)
                     /locus_tag="PLT69_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ14586.1"
                     /translation="MAKRFLTLVALLMLGFYVAGCAEGDESASVVNPNPSVFTPTGSI
                     SGVVVDACTGALIEGATVSVAYNGKVYKVKTSAQGQFTFNNVPANYEGPYFVSCDLGT
                     VTGDPYDGASFIEYAYVGYSDLNDGDNDSDVEESGSGADTPVDKLNSSIVFEVSNPTN
                     SVAGVVYDKTYGTNPLADATVALFDDDSNLISYATTAANGTFTISNVPAGDGYYLQVI
                     KSGYVYWNVTAASDCEGPDSSKLWLPCEVGCSSIPVNAGAIFVMQSVVRDNIIPFISN
                     IDVQIGTFAASGAFSGDKFMYDDEGDVLSPLVTSFGITFSEPMLTGHSSIYDAVQLEA
                     YLAIVVSTAGVEHVLTDTMLYSVDYDLAWDSTHQVLTLTPDITIADDNVLIRAGFGKN
                     RPTATVTYAAGYYRITFGEGAPSLSLADAHGCPWWYGGFSSNYMSVLSGIQSMILDDD
                     YFEILVGDEDYYDLIRKK"
BASE COUNT          524 a          892 c          885 g          558 t
ORIGIN      
        1 tgggcagcag gcgcccagtg gataccgcct tcagcgtcca aagacgggct cggcaccggc
       61 aggcaccagc tcgtgcccac gctcggcgcc aggtacgacc tggggggctg gatgcagggc
      121 gcctggtgcg gcgcccgtct cggccacgcc ttcgacgtgg ccgggtacga gggctacccg
      181 cacatcagcc agaccgtctt ccagcccatg ctgaacatcg acctgcccgg ctcctggttc
      241 ctcaccttct cgccagaggc gaggtacgac tggaagaacg aggaatggtt catacccttc
      301 gacatcacgg cgggcaagct cgcgcagaac gggctcgtgg tgctcttcac ctaccgatcg
      361 gccataaacg acgacatgct cctgtacgtg aacaccttcg agctgagggt gaggtacctc
      421 ttcgacgcgg acgtgcggac aatcctctca ggcgcatcct cagcggtgaa cgacgtgctc
      481 ggcgggacgc ccatggacat accggaaccc aagggcccat agatacacgc ccaaggcacg
      541 cttcatccct ttcggcaaga ccggccttgg ccggcatcag gccccgcatc gtcggttaag
      601 acagacggcg ctccgaccaa aggccgcaac agcccttccc cccgcacgca gggccctggc
      661 ggggcaccca gtgccggcat acaccacaag ccttgtgcct gaggcgaaga ggcgcacagc
      721 tccgatcgcc tccctttcca agatcctttg tgtcacgttc tgatgccgct gaggctgatg
      781 tgaggacagg accgtctcga tccagctccc cgaggcgcct cgcaccaagc actctccaca
      841 cccgagagcc gattccctgc cgttgaggcg ggtgagcccc tcgggcgcga tgtcaacgag
      901 ggcaccctgg ttgcttacga acagttcctc ggtgcttata tgggtgtctc cctgcggcac
      961 ggagtcagcc gaccacagtc acgttcgatt gcggtctctc acgccctgct tgctcatgtt
     1021 gggaggagac aggacccgca tcatcatctg cagagccgtt caccctccaa tgcccttgta
     1081 cgagcgcctg tccccctgcg gcgggttctt tcctcgaggt tgcggcttca ggtgagggct
     1141 ccgggcgtgc ttcgcgctct ctacgaggtc tttccccttc tgaactgcaa ggcggatcca
     1201 ggtggtgtca gtgaaggtgg gccaggtgta tcgaagaaaa aggatgtctc ttctccgctg
     1261 aaggaacggt cgtctcacgt gtcacgcgtt cacgccgccc ccttgaacca gaaaatacaa
     1321 atgagcgagg ggcgggtatc acacccgccc ctcgcagtgt tctcgtctca gacctcgttg
     1381 gccgaggcca aggaggtctg tctgcctttc actttttacg gatcagatcg tagtaatctt
     1441 cgtcgcccac gaggatctcg aagtaatcat cgtcgaggat catgctctgg atcccggaga
     1501 gcaccgacat gtagttgctg ctgaaaccac cgtaccacca cgggcagccg tgcgcgtccg
     1561 caagggagag cgacggggcg ccttcgccga aggtgatcct gtagtatccg gctgcatagg
     1621 tgacggtcgc tgtcggcctg tttttgccga agccggcacg gatcagcacg ttgtcgtcag
     1681 cgatggtgat gtccggggtg agggtgagca cctggtgcgt gctgtcccag gcgaggtcgt
     1741 agtccaccga gtagagcatg gtgtcggtca gcacatgctc gacaccggcc gtggagacca
     1801 cgatcgcgag gtaagcttcg agctggactg cgtcatagat gctggagtgc ccggtgagca
     1861 tgggctcgct gaaggtgatg ccgaaagagg tgacgagcgg ggagaggacg tcgccctcgt
     1921 cgtcatacat gaacttgtcg ccgctgaagg cgccgctcgc cgcgaaggtg ccgatctgga
     1981 cgtcgatgtt ggagatgaag gggatgatgt tgtccctgac cacgctctgc atgacgaaga
     2041 tcgcgccggc gttgaccggg atcgagctgc agccgacctc acagggcagc cagagcttgc
     2101 tgctgtcggg tccctcgcaa tccgaggcag cggtaacgtt ccagtagacg tagccggact
     2161 tgatgacctg gaggtagtag ccatcgcccg ccgggacgtt ggagatggtg aacgtgccgt
     2221 tcgcggcggt ggtggcgtag ctgatcaggt tgctgtcatc gtcgaacagc gccacggtgg
     2281 cgtcggcgag ggggttggtg ccgtaggtct tgtcatagac cacgcccgcc acggagttgg
     2341 tcgggttgct cacctcgaac acgatggagg aattgagctt gtcgaccggc gtgtccgcgc
     2401 cgctgccgct ctcctcgaca tcactgtcgt tgtcgccgtc gttcaggtcc gagtagccga
     2461 cgtatgcata ctcgatgaac gaagcgccgt cgtacgggtc accggtcacg gtgcccaggt
     2521 cgcagctcac gaagtagggc ccctcgtagt tcgccgggac gttgttgaac gtgaactgac
     2581 cctgcgcgct cgtcttcacc ttgtagacct tgccgttgta ggccacggac acggtggcac
     2641 cctctatgag agccccggtg cacgcgtcta ccacgacgcc cgagatggag cccgtgggcg
     2701 tgaagaccga aggattcggg ttcaccacgc tcgcgctctc atcgccctcc gcacagcctg
     2761 ccacatagaa gccgagcatg agcagtgcga ccaaagtcag gaacctctta gccatatctt
     2821 cctcctctac ttaaatcttt tttattttct ctgggacca
//
LOCUS       DAOSCR010000359         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22620_length_2858_cov_3.056011, whole genome shotgun sequence.
ACCESSION   DAOSCR010000359 DAOSCR010000000
VERSION     DAOSCR010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22620_length_2858_cov_3.056011"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..1810
                     /gene="selA"
                     /locus_tag="PLT69_08870"
     CDS             446..1810
                     /gene="selA"
                     /locus_tag="PLT69_08870"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOJ14587.1"
                     /translation="MNEAFRSIPKVDVVLSEPAVRDLPYDREIVKRFVKNRLDAIRGA
                     IASGSLSESPNAAAVALAVAAEARDLMETALVRVINATGVVVFTNLGRAPLAREASFA
                     ALEAAGGYTNLEYRLDQGRRGSRQDHIRPVARACFGAQDALVVNNNAAAVLLVLASLA
                     RGKEVIVSRGELVEIGGSFRMPDVMALSGAIMREVGTTNKTRLSDYRSALGENTGLIM
                     KVHRSNFSLVGFTDEVGLGELAALAREAGVPFFVDMGSGTPFDLSPWGITDEWTVPSC
                     LALGADVLCFSGDKVLGGPQAGIILGRSDLVERMARHPLHRAVRVDKFTIASLGATLR
                     LLAQGRTDSIPVLRMITEPETEVKRRATRLARLLKLEGARVVPTRAVVGGGSAPTKTF
                     ASYGVALRHESANRVHAGLRAARPAVVCRIEEDMLVFDLKAVDRQEIPVLARIIREVT
                     SHAV"
     gene            1800..>2244
                     /locus_tag="PLT69_08875"
     CDS             1800..>2244
                     /locus_tag="PLT69_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ14588.1"
                     /translation="MPYEVGVALGGGGVRGLANVGALKALLDAGVKPACVSGTSMGAI
                     VGSLYAASLDVKEVERTLRRAFTSEEFRRRVERFAGSGSDLDRGFMEKVLESVRKGYF
                     FYRFMFRESVVAEGAFLEGMERIVPDMNFSELMIPFACMTLDLVSG"
     assembly_gap    2245..2254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2255..2722
                     /locus_tag="PLT69_08880"
     CDS             <2255..2722
                     /locus_tag="PLT69_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ14589.1"
                     /translation="ILRSGSVRLAVRASSAVPGFMPPVRVEHMVCVDGAWAESVPVSA
                     ARVLGARFVVGVDVSRDIEPIDGSRDIDNSLDILVRAGDLTRLFMNTLRTAQADFVIH
                     PEVGDAAWNAFDDIDAYIAAGYSEAAGRIPALKKALRVHRIKEALGLAKKRGS"
BASE COUNT          449 a          892 c         1045 g          462 t
ORIGIN      
        1 tcggcgtgag gccgcccccc gagacgttct cgtagcaggc cacgtcgaag tccgctgcga
       61 gctccctgag gccccaccag tagtcgagcg cggtgaggtc cttgtacttc tcctccttgt
      121 ccttgccgtg acagaggtgg cggtcgatca tccgtggctt cgccatctat gttcctctcg
      181 tgccctgccg gccggtggtg cgtcgtttcc ccggctgtcc ttcaccatga ataaagggaa
      241 aggaaatccg ttgcaagagg attccgcccc catgcgtccc aggctgcgcc cgggacctgg
      301 gtgatgcctg gcgccggggg ggtgcggcgg ggcgcgaaaa gcggagcgcg gcttgactcc
      361 cggccgtggt atggcatact tgcctggagg gttcttccgg ggcgctgcac gaccgggccc
      421 gtccgtagtg tgaggggagg tttgcgtgaa cgaggcattc aggagcattc cgaaggtgga
      481 cgtcgtgttg tccgagccgg ccgtgagaga cctgccctac gaccgggaga tcgtcaagcg
      541 cttcgtgaag aacaggctgg atgcgatcag gggggcgatc gcatccggca gccttagcga
      601 gagcccgaac gcggccgcag tggctctggc ggtggcggcc gaggcccgcg acctcatgga
      661 gaccgccctg gtgcgcgtga tcaacgcgac cggcgtggtg gtgttcacca acctcggcag
      721 ggcgccgctc gccagggagg catcctttgc ggcgctcgag gccgcaggcg ggtacacgaa
      781 cctcgagtac cggctcgacc agggcaggcg cggttcccgg caggatcaca tccgccccgt
      841 ggcgagggcc tgcttcggcg cacaggacgc gctcgtggtc aacaacaacg cggccgcggt
      901 gctgctggtg ctcgccagcc tcgcgagggg aaaggaagtc atcgtctcgc ggggcgagct
      961 cgtcgagata gggggctcgt tccgcatgcc ggatgtcatg gcgctcagcg gcgcgatcat
     1021 gcgggaggtg ggcaccacga acaagacgag gctgtccgat taccggtcgg ccctggggga
     1081 gaacaccggg ctcatcatga aggtgcaccg gagcaacttc tcgctcgtgg ggttcaccga
     1141 cgaggtcggc cttggcgagc tggccgctct cgcgcgggag gcgggggtgc ccttcttcgt
     1201 cgacatgggc tcgggaacgc ccttcgacct ctcgccgtgg gggatcacgg acgagtggac
     1261 cgtaccctcg tgcctcgccc tgggcgcgga cgtcctctgc ttcagcgggg acaaggtcct
     1321 gggcgggccc caggctggaa tcatcctggg caggtccgac ctggtggaaa ggatggcgag
     1381 gcacccgctg caccgcgcgg tgcgcgtcga caagttcacc atagcgtccc tgggcgcaac
     1441 cctgaggctg ttggcccagg gcaggacgga cagcataccg gtgctcagga tgatcaccga
     1501 gcccgagacc gaggtaaagc gacgcgccac caggctcgcc cgtctcctga agctcgaggg
     1561 ggcgcgcgtc gtgcccacca gggcggtggt gggcggcggt tcggcgccga cgaagacctt
     1621 cgcctcctac ggcgtcgcgc tcaggcacga gtcggcgaac agggtacacg caggcctcag
     1681 ggccgccagg cccgcggtgg tctgccgcat cgaggaagac atgctcgtgt tcgacctcaa
     1741 ggcggtcgac cggcaggaaa tcccggtgct cgccaggatc atacgcgagg tgaccagcca
     1801 tgccgtatga ggtgggcgtg gccttgggcg gcggcggggt gaggggactc gcgaacgtcg
     1861 gcgccctgaa ggccttgctc gatgccggcg tgaagcccgc ctgcgtctcg ggcacaagca
     1921 tgggcgccat cgtgggatcc ctgtacgccg cgagcctcga cgtgaaagag gtggagcgca
     1981 ccctcaggag ggcgttcacc tcggaggaat tcaggcgcag ggtggagcgg ttcgcaggct
     2041 ccggttccga ccttgaccgc ggcttcatgg aaaaggtcct cgagagcgta cgcaagggat
     2101 acttcttcta ccgtttcatg ttcagggaat cggtggtggc cgagggcgcg ttcctcgagg
     2161 gcatggagag gatcgtgccg gacatgaact tctcggagct catgataccc ttcgcgtgca
     2221 tgacgctgga cctcgtctcg ggccnnnnnn nnnnatcctg cgctcgggca gcgtcaggct
     2281 cgcggtcagg gcctccagcg cggtgcccgg gttcatgccg ccggtgcgcg tggagcacat
     2341 ggtgtgcgtg gacggcgcgt gggccgagtc ggtccccgtc tctgcggcca gggtgctggg
     2401 cgcgcgcttc gtggtgggcg tcgacgtgtc gagggacatc gagcccatcg acggcagccg
     2461 ggacatcgac aacagcctgg acatactcgt gcgcgcaggc gacctcacga ggctcttcat
     2521 gaacaccctc aggacggcgc aggccgattt cgtcatccac cccgaggtgg gcgacgccgc
     2581 gtggaacgcg ttcgacgaca tcgacgccta catcgccgca ggctacagcg aagcggccgg
     2641 gaggatcccg gccctgaaga aggccttgcg cgtgcaccgg atcaaggagg cgctgggcct
     2701 ggcgaagaag cggggctcgt gaggtgcggg aagacaaaga tgtgacgcgt aaacccctgg
     2761 aggcggacgc cggcgacgtc cggggcccgg ccggtccttc ccccggggga cgcatcctcg
     2821 cggcgggcgc ggcagtgctc gtctcggcgg tgatcgtt
//
LOCUS       DAOSCR010000360         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22645_length_2856_cov_2.937165, whole genome shotgun sequence.
ACCESSION   DAOSCR010000360 DAOSCR010000000
VERSION     DAOSCR010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22645_length_2856_cov_2.937165"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="lpxB"
                     /locus_tag="PLT69_08885"
     CDS             complement(<1..857)
                     /gene="lpxB"
                     /locus_tag="PLT69_08885"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOJ14590.1"
                     /translation="MRKTVLIAAGEASADRHASAVIRAVRALVPEAAFVGLGGPEMKS
                     AGMDAVYSMEEISIMGFTEVASRIFGILRVYRGMKRLIRELRPDLFIAVDLPDFNMRL
                     ASYAKSLGVKVLYYIAPQAWAWRRSRAATLARITDGLAVIFPFEEGFFKAHGVHARYV
                     GHPMLEPGSGEVPEASQTRWPPESICMMPGSRAQEIRMILPVMTQAKRLVSRGFPGIR
                     WQLRLAPGLDRAFVERFTDADVLVEAGARPADLAIVKSGTSSLEMAVSGVPEIVCYRT
                     STVNYLLARL"
     gene            complement(854..1780)
                     /locus_tag="PLT69_08890"
     CDS             complement(854..1780)
                     /locus_tag="PLT69_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ14591.1"
                     /translation="MSRLRVAVVGVGYLGRFHAQKYAAMDDVDLVAVVDVDQGRAQEV
                     ARELGTVACTSIDHIGRPLDAASVAVPTSSHAEVAVRLLERGVAVLVEKPMASSLADA
                     RAIVEASRKTGAILQVGHLERFNPALVKLKHAISEPMFIEAHRISPYRSRGTDVDVIL
                     DIMIHDLDIILDLVPSPVASIEAVGVNVLTEGVDIANARLRFANGCIANITASRVSAD
                     VMRKIRIFQKNSYISIDFAKAKADVYTLSPDRRIEHEHYAMSESDALAAEIRSFVDAV
                     RERTEVAVDGEAGLRAMEAALLVKEHLVVPGK"
     gene            complement(1777..2553)
                     /gene="lpxA"
                     /locus_tag="PLT69_08895"
     CDS             complement(1777..2553)
                     /gene="lpxA"
                     /locus_tag="PLT69_08895"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOJ14592.1"
                     /translation="MPTIDPTAKVSPKAVLADDVTVGPYAVIAAEVSIGPGTYVGPFC
                     TIEGPTVIGAGCTFTGYASVGTPPQDHSYRGEPTRLTMGDNNTIREFVTINRGTSKDR
                     GETVIGSGNLVMAYCHVAHDCVLGDHVVMGNLATLAGHVQIHDHAIIGGMSAVHQFTR
                     VGAYAIVGGGSMVSLDIVPYARASGDRARLFGVNTVGLKRSGFKPEEISRIERGFRIL
                     FRQGLLLREAIEHLEREFPNAPEIGRLLEFLRNTRRGIAR"
     gene            complement(2540..>2856)
                     /locus_tag="PLT69_08900"
     CDS             complement(2540..>2856)
                     /locus_tag="PLT69_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOJ14593.1"
                     /translation="HNVVIGDHTIIVAQTGIAGSARIGSYVVLGGQCGVAGHISVGDG
                     VKAAARTGIASDVEPGRVVSGFPSMDHSRWLRVQAAYKDLPSLLKRVRDLERKVMERA
                     DD"
BASE COUNT          442 a          978 c          939 g          497 t
ORIGIN      
        1 agcctcgcga ggaggtagtt gacggtcgag gtgcggtagc agacgatctc gggcacgccc
       61 gagaccgcca tctccaggga agaggtcccg gacttcacga tggcgaggtc cgcggggcgg
      121 gcccccgcct cgacgagcac gtccgcgtcc gtgaagcgct ccacgaaggc cctgtcgaga
      181 ccgggggcga gcctgagctg ccaccggata cccgggaacc ccctcgatac gagtctcttg
      241 gcctgcgtca tcaccggcag gatcatccgt atctcctgcg ccctgctccc cggcatcatg
      301 cagatgctct cgggcggcca gcgggtctgg gaggcctcgg ggacctcgcc cgagcccggc
      361 tcgagcatgg ggtggcccac gtagcgggca tgcacgccgt gggccttgaa gaacccctcc
      421 tcgaagggga agatcaccgc gaggccgtcg gtgatcctgg ccagggtcgc ggcccgcgac
      481 cttctccacg cccatgcctg cggcgctatg tagtagagga ccttcacgcc gagggacttg
      541 gcgtaggatg cgagcctcat gttgaagtcc ggcaggtcga cggctatgaa caggtcgggc
      601 cgcaactccc tgatgaggcg tttcatcccc cggtagaccc tcaggatgcc gaagatacgg
      661 gatgcgacct cggtgaaccc catgatggag atctcctcca tggagtagac cgcgtccatg
      721 cccgcagact tcatctcggg cccgccgagg ccgacgaagg cggcctcggg gacgagcgcc
      781 cggacggccc tgatgacggc ggatgcgtgc ctgtccgcgc tcgcctcacc cgctgcgatg
      841 agaacggtct tcctcatttc cccggcacca cgaggtgttc cttgaccagg agcgcggcct
      901 ccatggccct gagcccggcc tcgccgtcga ccgcgacctc cgtcctctcc ctgacggcgt
      961 ccacgaaaga cctgatctct gcggccaacg cgtcggactc gctcatcgcg tagtgctcgt
     1021 gctcgatgcg gcgatcgggg ctcaaggtgt acacgtcggc cttggccttt gcgaagtcga
     1081 tggagatgta ggagttcttc tggaagatcc tgatcttccg catgacgtca gcgctcaccc
     1141 tgctcgcggt gatgttcgcg atgcacccgt tcgcaaagcg cagcctcgcg ttcgcgatgt
     1201 ccacgccctc ggtgagcacg ttcaccccga ccgcctcgat ggacgcgacg ggagagggga
     1261 cgagatcgag gatgatgtcg aggtcgtgta tcatgatgtc gaggatgacg tccacgtccg
     1321 tgccccgcga gcggtagggg ctgatgcggt gcgcctctat gaacatgggt tccgatatgg
     1381 cgtgcttgag cttcacgagc gccgggttga aacgctcgag gtggcctacc tggaggatcg
     1441 cgcccgtttt ccgtgaggcc tccacgatcg cgcgcgcatc agcgaggctc gacgccatgg
     1501 gcttttccac gagcacggcc acccctctct cgaggagcct caccgccacc tccgcgtggg
     1561 aggacgtcgg taccgccacg ctcgccgcgt ccaggggcct gccgatgtgg tcgatcgagg
     1621 tgcacgccac ggtgcccagc tcccgggcga cctcttgcgc gcgcccttgg tccacgtcca
     1681 cgacggccac gaggtcaacg tcgtccatgg cggcgtactt ctgcgcgtgg aagcgtccca
     1741 ggtagcccac ccccacgacc gcgaccctga gcctgctcac ctcgcgatcc ccctcctggt
     1801 gttcctcagg aactcgagca gccgccctat ctccggggcg ttcgggaact ccctctcgag
     1861 gtgctcgatc gcctccctga ggagcagtcc ctgcctgaag aggatgcgga acccccgttc
     1921 gatccttgag atctcttcag gcttgaagcc ggagcgcttc agcccgacgg tgttcacgcc
     1981 gaagagcctc gccctgtcgc cggaggcccg ggcgtacggc acgatgtcga ggctcaccat
     2041 ggaaccgccg cccacgatgg cgtaggcgcc cacgcgggtg aactggtgca ctgcggacat
     2101 cccgccgatg atcgcgtgat cgtggatttg gacgtggccg gcgagcgtcg ccaggttccc
     2161 catgaccacg tgatctccca ggacgcagtc gtgggcgacg tggcagtagg ccatgacgag
     2221 gttgccgctg ccgatgacgg tctccccccg gtccttggac gtgccccggt tgatggtgac
     2281 gaactcccgg atggtgttgt tgtcgcccat ggtgaggcgt gtgggctccc ccctgtacga
     2341 gtggtcctgg ggaggggtcc cgacgctcgc gtagcccgtg aaggtgcacc cggcgccgat
     2401 gacggttggc ccctcgatgg tgcagaacgg gcccacgtac gtcccggggc cgatgctcac
     2461 ctcggcggcg atcacggcgt acggccccac ggtgacgtcg tccgcgagaa ccgccttggg
     2521 cgagaccttt gcggtcgggt caatcgtcgg cacgctccat caccttcctt tccaggtcgc
     2581 gcacccgctt gaggagcgag ggcaggtcct tgtacgctgc ctgcaccctg agccagcggg
     2641 agtggtccat ggaggggaac cccgatacga cgcggcccgg ctccacgtca gaagcgatgc
     2701 ccgtcctggc cgcggccttg accccgtctc cgacgctgat gtgacccgcg accccgcact
     2761 ggcctccgag caccacgtag gagcctatgc gggcgctgcc ggcgatgccg gtctgggcca
     2821 cgatgatcgt gtgatcgccg atcacgacgt tgtgac
//
LOCUS       DAOSCR010000361         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22683_length_2853_cov_2.771980, whole genome shotgun sequence.
ACCESSION   DAOSCR010000361 DAOSCR010000000
VERSION     DAOSCR010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22683_length_2853_cov_2.771980"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLT69_08905"
     CDS             complement(<1..1095)
                     /locus_tag="PLT69_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ14594.1"
                     /translation="MADVVVIGAGPAGIFASMELVKTSPGIDLVMVEQGSDIDTRGRT
                     GRDMLVGWGGAGAYSDGKLTVSTDVGGQLSAIMPEDDLLDLLTYVDGIYERRSPGSAL
                     FAMDPEEVASMAAKARLAGLVFVPARVRHLGTEHLRTILEVFRRELEGKARMLFGVRA
                     AEILADKGRVTGVRLEDSSVIPARYVIAAPGRVGSAWMRQQAHRLGLESRPNPVDVGV
                     RVELPAVVLEPLTSRAYETKFVHFSRTFDDKVRTFCMNPYGEVVIETVGDLVTVNGHS
                     WATKRTENTNFALLVSANFTEPFDDPIGYGESVARLANMLGKGVIVQRLGDLLDGRRS
                     TAERIRRGLVRPTMESATPGDLSLVLPYRHL"
     gene            complement(1088..2167)
                     /locus_tag="PLT69_08910"
     CDS             complement(1088..2167)
                     /locus_tag="PLT69_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ14595.1"
                     /translation="MGTLSRYIAARTTLMCTGALATLTTLVVASVFLGNMGTFAEHST
                     PASVVLRFILYSVPQILYWVIPFSVCVGVVAAQAAFARHVETIAMQACTVSFRRMCVP
                     YVLVATAAVVLMGVMSFDVYPLAQREADRIEQVLIKKRGVEGSFTVHGGRFKVGPEIY
                     LVRYADVVHGVMRDVRCYSLAGGAISTVRRADMVRWTGDGWVSDDMEVLELSRGAIVS
                     KRGKYSLPLRHRPEDLAVAQPSAEVLTLSELLEYRKSLARESIRSLSLDTQIHNRVSF
                     TMAPLVMTLLVLPFGLRFPRAGGIARGITLGIVTGLVYWSAHSAMVGAGMAGYVHPVP
                     AAWSVNVAALFASALIMKHRRFSYG"
     gene            complement(2155..>2853)
                     /locus_tag="PLT69_08915"
     CDS             complement(2155..>2853)
                     /locus_tag="PLT69_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ14596.1"
                     /translation="DLMRSAPRKMLAFFKEREFNDRFRDLVLYVESVNQRKRQLKGVF
                     LEGSKPGRFVVSARRGNVEIDGTRVTLILKEGGMFSSAGDADRYLTFDEYSFTLQADL
                     EGMLRIKSYDTATQGEFRRLIEQDPSPKRVREYHNRYAFPVINILLAAIGIVFGVQNP
                     RSPRYTGFLIGMATIVAYYLSNVMSARLVAARVMDPVLGAWLADICFATFLVLVVSFR
                     RLVAVAKRGRAWAR"
BASE COUNT          524 a          978 c          869 g          482 t
ORIGIN      
        1 cagatggcgg tacggcagga ccagggacag atcgccaggc gtagcgcttt ccatagtcgg
       61 ccggaccagg ccgcggcgga tccgctctgc cgtggaccga cggccatcca acaaatcgcc
      121 caggcgctgg acgatgaccc ccttgccgag catgttcgcg agcctggcca cggactcccc
      181 gtagcctatg gggtcgtcga agggttccgt gaagttcgcg ctcaccaaca gcgcgaagtt
      241 ggtgttctcg gtcctcttcg tggcccagga atgtccgttc accgtcacga gatcccccac
      301 ggtctcgatc accacctcgc catagggatt catgcagaac gtcctcacct tgtcgtcgaa
      361 ggtgcgggaa aagtggacga acttggtctc gtacgccctc gaggtgaggg gctcgaggac
      421 cacggcgggg agctccaccc tcacgcccac gtccacgggg ttcggcctcg attcgagccc
      481 gagcctgtgg gcctgctgcc tcatccacgc cgagcccacc ctgccgggcg cggcgatcac
      541 gtaccgtgcg gggatgacgc tcgagtcttc gagcctgacc ccggtcaccc tccccttgtc
      601 cgccaggatc tcggcggcgc ggacgccgaa gagcatcctt gccttcccct cgagctcgcg
      661 cctgaacacc tcgagtatgg tgcggaggtg ttccgtcccg agatgcctca cccgtgcggg
      721 cacaaagacg aggcccgcaa gcctggcctt ggccgccatg gatgcgacct cttccggatc
      781 catggcgaag agggcgcttc cgggcgagcg cctctcgtag atcccgtcga cgtaggtaag
      841 gagatcgagg agatcgtcct cgggcatgat cgccgagagc tgccccccca cgtcggtcga
      901 cacggtgagc tttccgtcgc tgtacgcgcc cgctccgccc caccccacga gcatgtccct
      961 gcccgtcctg ccgcgggtgt cgatgtcgct gccctgttcc accatgacca ggtctatgcc
     1021 tggagacgtc ttgacgagct ccatagaggc gaagataccg gcgggcccgg caccgatcac
     1081 gacgacgtca gccataggaa aacctcctgt gtttcatgat caaggcggat gcgaagaggg
     1141 ccgcgacgtt gaccgaccat gcggccggca cgggatgcac gtagccggcc atgccggccc
     1201 ccaccatggc cgagtgcgcg gaccaataga cgagacccgt gacgatgccc agggttatac
     1261 ccctcgcgat ccctcctgcg cgggggaaac gcaggccgaa gggaaggacg agcagggtca
     1321 tgaccagggg ggccatggtg aaactcaccc ggttgtggat ctgcgtatcg aggctcaagg
     1381 accgtatcga ctcccttgcg aggctcttcc tgtactcgag gagctcggag agggtcagca
     1441 cctcggccga cggctgggcg acggcgaggt cttccggacg gtgcctcagc gggaggctgt
     1501 acttgcccct cttcgagacg atcgcccccc gggacagctc gagcacctcc atgtcatctg
     1561 agacccaccc gtctcccgtc cacctgacca tgtccgccct tctcacggtg ctgatcgcac
     1621 cccctgcgag gctgtagcac ctgacgtccc tcatgacgcc gtgaaccacg tccgcatacc
     1681 tcacgaggta aatctcgggg cccaccttga acctgccccc gtggacggtg aacgacccct
     1741 cgacccccct cttcttgatg agcacctgct ctatcctgtc agcctccctc tgcgccaggg
     1801 gatagacatc gaaggacatc acccccatga gcacgacggc cgcggtggcg acgaggacgt
     1861 atgggacgca catcctccgg aaagacaccg tgcacgcctg catggctatg gtctcgacgt
     1921 ggcgggcgaa cgccgcctgg gcggccacga cacccacgca gacggaaaag gggatgaccc
     1981 agtacaatat ctgcgggaca gagtagagga tgaacctcag caccacggag gcaggggtgg
     2041 aatgctcggc gaacgtcccc atgttgccca gaaacacgct cgcgaccacc agggtcgtca
     2101 gcgtcgcgag cgcccccgtg cacatgagcg tggtcctcgc cgcgatgtac ctgctcagcg
     2161 tgcccatgcg cgacccctct tcgcgaccgc gacaagccgc ctgaagctca ccacgaggac
     2221 gagaaacgtg gcgaagcaga tgtccgcaag ccacgcgccg agaacggggt ccatgacgcg
     2281 ggcggccacg agccttgccg acatgacgtt cgagaggtag tacgccacga tcgtggccat
     2341 gcctatgaga aaacccgtgt accggggcga acgcgggttc tgcacgccga agacgatgcc
     2401 gatcgccgcc aagaggatgt tgatgaccgg gaaggcgtaa cggttgtggt attcccgtac
     2461 gcgcttcggc gacgggtcct gctcgatgag ccgcctgaat tcgccctgcg tcgcggtgtc
     2521 gtagctcttg atcctgagca tgccctcgag gtccgcctgg agggtgaacg agtactcgtc
     2581 gaaggtgagg tacctgtccg catcccccgc cgaggagaac atgccgccct ctttcagtat
     2641 caaggtcacc ctcgtcccgt ctatctccac gtttcccctc cgtgcggaga ccacgaaacg
     2701 gccgggcttc gagccctcga ggaacacccc cttgagctgc ctcttcctct ggttcacgga
     2761 ctcgacgtac agcacgaggt ccctgaacct gtcgttgaac tcgcgctcct tgaagaacgc
     2821 gagcatcttc ctcggcgcag acctcatcag gtc
//
LOCUS       DAOSCR010000362         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22697_length_2852_cov_2.407222, whole genome shotgun sequence.
ACCESSION   DAOSCR010000362 DAOSCR010000000
VERSION     DAOSCR010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22697_length_2852_cov_2.407222"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1136
                     /locus_tag="PLT69_08920"
     CDS             117..1136
                     /locus_tag="PLT69_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ14597.1"
                     /translation="MRDTVVILHQEVGHEAPADELDVLDQVECVERELRDMGYRVRTL
                     PFDLDLRNLARRIHKLHPALAFNLVETVMGDGRLAPLVCSFLDHLGIPYTGSGAQAML
                     LTTNKTMAKRWMGPWGIPTPEWFEPGRPSEGAPPFPATYILKPVWEDASVGIEAGCVI
                     EAEETSALVKALHDLERSIHKPCFAERFIDGREFNLALLACGDSVEVLPVCEIVFDLP
                     EGMPRIVGYRAKWAEESVEFKGTVRVTDFPGEDSALLERLRRTALACWDLFDLRGYAR
                     VDMRVDREGRPFVLEINANPCISPDSGFCAAASKAGLAFRDIVERIVQAAARPPVQGR
                     LEPCR"
     gene            1127..1624
                     /locus_tag="PLT69_08925"
     CDS             1127..1624
                     /locus_tag="PLT69_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ14598.1"
                     /translation="MQVGGLTPGFRHEVRSGDIESVRCIVSATGFFTEEEIAVAMELV
                     ETALSDQASSPYRFLFAETEPEGVVGYTCFGRIPCTASSFDLYWIAVTPACQGRGIGK
                     ALMGATEEVIRGMGGERIYIETASRALYRPTRLFYERCGYVPEAMLADFYSRGDGKII
                     YVKRL"
     gene            2052..2528
                     /locus_tag="PLT69_08930"
     CDS             2052..2528
                     /locus_tag="PLT69_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOJ14599.1"
                     /translation="MDVNLINPFIEATLHVLSSLAFTQAKAGKPYLKKDQMARGDVSG
                     IVGLSGEARGTLSVSFSERSILSIVSNMFGEPVHEINDEVKDAVGEILNIVSGQARQK
                     LESMGRSLKGAIPTVITGKNHTISHITKQPIIAIPFDTDNGHFTIEVCIEEKSQPG"
BASE COUNT          514 a          940 c          938 g          460 t
ORIGIN      
        1 ctcaacccgg tgcactccga cctgccgatc gtgtgcaggc tcgcgggcat ggattaccgg
       61 gagctcatcg gccgcatcct ggagagcgcg gtgatgcgca tggagggcgg gtccgggtga
      121 gagacacggt ggtgatcctc caccaggagg tgggccacga ggccccggcg gacgagctcg
      181 acgtcctcga ccaggtggag tgcgtggaac gggagctcag ggacatgggg tacagggtcc
      241 gcaccctgcc cttcgaccta gacctgagaa atctcgcgag gcggatccac aagctccatc
      301 ccgcgctggc gttcaacctc gtggagacgg tcatgggcga cggcaggctc gcacccctgg
      361 tctgttcttt cctcgatcac ctggggatcc cgtacacggg ctcaggggcg caggccatgc
      421 tcctcaccac gaacaagacc atggcgaagc ggtggatggg tccctggggc atccccacgc
      481 ccgagtggtt cgagcctggg cgtccctcgg aaggtgcgcc gcccttcccc gcgacataca
      541 tcctgaagcc ggtctgggag gacgcttccg tggggatcga ggcagggtgc gtgatcgagg
      601 ccgaagagac gagcgctctc gtcaaggccc tgcacgatct cgagcgctcg atccataagc
      661 cgtgcttcgc ggagcgtttc atagacggcc gcgagttcaa cctcgcgttg ctcgcatgcg
      721 gggatagcgt cgaggtgctg cccgtttgcg agatcgtctt cgacctgccc gagggcatgc
      781 cccggatcgt gggctatagg gccaagtggg cggaggagag cgtcgagttc aaggggaccg
      841 tgagggtgac cgactttccc ggcgaggact cggcgctctt ggagcggctg aggcggacag
      901 cccttgcgtg ctgggacctc ttcgatctca gggggtacgc ccgggtggac atgcgcgtcg
      961 acagggaagg caggccgttc gtcctcgaga tcaacgcgaa cccgtgcatc tccccggaca
     1021 gcgggttctg cgcggcggcc tccaaggccg gcctcgcctt cagggacatc gtggagcgca
     1081 tcgtccaggc cgcggcgaga ccccccgtcc aagggcggct ggagccatgc aggtaggcgg
     1141 actcaccccg ggcttcaggc acgaagtgag aagcggcgac attgagtcgg tgagatgcat
     1201 cgtgagcgcg accggcttct tcacggaaga ggagatcgcc gtcgccatgg agctcgtgga
     1261 gacggccctc tcggaccagg cgtcctcgcc ctaccgattc ctgttcgcag agaccgagcc
     1321 cgaaggggtc gtgggctaca cctgtttcgg caggatcccc tgcacggcgt catccttcga
     1381 cctctactgg atcgcggtca cgcccgcgtg ccagggcagg ggcatcggca aggcgctcat
     1441 gggcgcaacc gaggaggtca tccgcggcat gggcggtgag cgcatctaca tcgagaccgc
     1501 atcccgggcg ctctaccggc cgacgcggct cttctacgag aggtgcggct acgtccccga
     1561 ggccatgctt gcagacttct actccagggg agacggcaag atcatctacg tcaagcgact
     1621 gtgatgaacc cgggcgctgt cgagcccggc acacctgcat tccaccccct gggtgccccg
     1681 ccggtcgcct gttccctgtc ccgcctccgt gaagcgagaa cgagcggcaa gggcgcccct
     1741 tcccgacatg gtccgtgcgg acgacgatct cccgcccctt tcgcggcgac cgggcgcggc
     1801 cttgggacat cgggacccct ggcgcttgcg ctcaacaagg ccgaggagaa gtcgcccggg
     1861 caggccccgg ttgggttcgg caaggcgggg aaaccggtgg gccggcttgc acgtcctctc
     1921 acccctcagg gtcgtcccgc ggcacaagac cagaggtccc gtccatcgcc ggcatccaca
     1981 ttccccttta ctttggcgac ggaggtgccg ataccccctg tagcgcacct ataccaaaca
     2041 atggagcacg tatggacgtg aatctcatca acccgttcat cgaggcgacg cttcatgtcc
     2101 tgtcgtcgct ggccttcacc caggcgaagg ccgggaagcc ctacctgaaa aaggaccaga
     2161 tggccagggg ggacgtctcg ggcatcgtgg gcctgtctgg cgaggccagg gggaccctgt
     2221 cggtgagctt ctccgagcgg agcatcctct ccatcgtgag caacatgttc ggcgaaccgg
     2281 tccacgagat caacgacgag gtcaaggacg cggtgggcga gatcctcaac atcgtctcgg
     2341 ggcaggcacg acagaagctc gagtccatgg gacgctcgct caagggcgcc atccccacgg
     2401 tgatcacggg caagaaccac accatcagtc acatcacgaa acagccgatc atcgccatcc
     2461 ccttcgacac cgacaacggc cacttcacca tcgaggtctg catagaggag aagtcccagc
     2521 ccgggtgaga gggccgctcc ggcaagccct cctcgggcgg gcaagccttg caataccgat
     2581 gatccgcaga cgtccgcggt gggggcgcca cctcggggga cgcaaagaaa ccgtgtcccg
     2641 tgtcatctta tcacccgacc ccgggtgtcc cgccgggccc cgggataccg attgagaccg
     2701 gcaaaccact tagtaaaggc cgaccgcgag gcttccgctt gccttgacca gacaccatga
     2761 tcgcgacctg atgccacgac acagagggtc agacccctgg ccaggcgttc ctctcctgta
     2821 gggaagaacg gagcctatcc ccctgggggc tc
//
LOCUS       DAOSCR010000363         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22705_length_2851_cov_3.040415, whole genome shotgun sequence.
ACCESSION   DAOSCR010000363 DAOSCR010000000
VERSION     DAOSCR010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22705_length_2851_cov_3.040415"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..677
                     /locus_tag="PLT69_08935"
     CDS             54..677
                     /locus_tag="PLT69_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ14600.1"
                     /translation="MRRCIDAVLFDFGGVLAEEGFRNGLYEIASRNGMDPAAFFETAR
                     LLIASTGYLTGRSTEARYFAELRRRTGLREDDVTMRKTILDGFVMRPWMVDLVKRLAE
                     NWIRTAVLSDQTDWLDELEGRLGFSRLFERVFNSYHMGKSKSDPSLFKDVLSCMGLEA
                     ARTLFVDDTEGHVERARSVGINAVHYREREQAFREISSFFPGIRLVT"
     gene            complement(806..2197)
                     /locus_tag="PLT69_08940"
     CDS             complement(806..2197)
                     /locus_tag="PLT69_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ14601.1"
                     /translation="MGNQPDRAVLVVDDDLGHRTMLKTLLGGWDFKVSEASDGQEAID
                     AVRDAPFDLVLMDIRMVKVSGLEALASIKEINPAIPVIIMTAYSSVETAIEAMKKGAY
                     DYLTKPLDFDALRLKMEQAMEHSSLKEENRILKSLVGERFDSSNIIGRSPAMVRLLET
                     AQRASASDATVLVSGESGTGKEMVAGAIHYNSSRKDGPFIKINCAAISEGLLESELFG
                     HEKGAFTGALRRREGSFKQAHRGTLFLDEVSEMSLAMQAKLLRVIQEREFTRVGGEEV
                     IRVDVRIIAATNRDLAQMVRERTFREDLFYRLNVIHLSVPPLRERREDIPLLAQHFLG
                     LFTKKNAKDIKGFTPQAMDRLIRHTWPGNVRELMNTIESAVVLSSTPNLREEDITLVY
                     PGGEDAVPQAIPAELTLDEVERSTILAVLSSVGGNKSEAARKLGITRATLHKKLKSYG
                     VEGENLHGPSRRA"
     gene            complement(2209..>2851)
                     /locus_tag="PLT69_08945"
     CDS             complement(2209..>2851)
                     /locus_tag="PLT69_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14602.1"
                     /translation="SLAAGVAHEIRNPLSSIKGFATYFRERYHMVPEDARIADIMIGE
                     VERLNRVIGQLLDLAKPLDMKKERCSLLSIVRRTLETVEDQAARRSITIDRSGLGENP
                     CHVVADPDKIAQVMLNLYLNAMEAMDSGGVLSVALECNGIDSPVTIEVSDTGCGIHPE
                     HLEHIFDPYFTTKQSGTGLGLAVVHKIVEAHGGDIKVTSRMGQGTTVRVTLGP"
BASE COUNT          470 a          869 c          923 g          589 t
ORIGIN      
        1 gtggttttcg ggcggtttcc tcaggaatat gatcatgtga ggggagggtc cctatgcgga
       61 ggtgtataga tgccgtgctc ttcgacttcg ggggagtcct tgccgaggaa gggttcagaa
      121 acggcctgta cgagatcgct tcgagaaacg gtatggatcc ggcggcattc ttcgagaccg
      181 cgcgtctcct catcgcctcg acagggtacc tgacaggccg ctcgaccgag gcccggtact
      241 tcgcggagct caggagacgc acgggattga gggaagacga tgtcacgatg aggaagacga
      301 tcctcgacgg gttcgtgatg agaccctgga tggtggacct cgtgaagagg ctcgcggaaa
      361 actggatacg cacggccgtc ctcagcgacc agacagactg gctcgacgag ctggaggggc
      421 gactcggctt ttctcgcctg ttcgaacgtg tcttcaacag ctaccacatg gggaaatcca
      481 agagtgaccc gagcctgttt aaggacgtgt tgtcctgcat ggggcttgaa gcggcaagga
      541 ccctcttcgt ggacgacacc gaggggcacg tggagagggc ccgctccgtg ggcatcaatg
      601 ccgttcacta cagggagagg gaacaggcgt tcagggagat ctcctccttc tttcccggga
      661 tccggctcgt aacctgaggg cgccttgcgc gggaggcccc gaggtgcccg gaggctggcc
      721 cgtcctcaag ggtcactgcc agggtccggg gaccttctct cttcacggcg cggcacaccc
      781 gcatcgccga ggacgaggcc cgtggtcatg cacgacgtga ggggccatga aggttctctc
      841 cttccacccc gtagctcttc agcttcttgt ggagcgtcgc cctggttatc cccagctttc
      901 tggccgcttc gctcttgttg ccgccgacgg aagagaggac ggcgaggatg gtcgaacgct
      961 ccacctcgtc gagggtgagt tcggcgggga tggcttgggg aacggcgtct tccccacccg
     1021 ggtagacgag cgtgatgtcc tcttccctga ggttgggggt cgaggagagc accacggcgc
     1081 tctcgatggt gttcatgagc tccctgacgt tgcctggcca cgtgtgcctg atgagacggt
     1141 ccatggcctg gggtgtgaac cccttgatgt ccttcgcgtt cttcttggtg aagagcccga
     1201 ggaagtgctg tgcgagaagg gggatgtcct cccttcgctc acgaaggggc ggcacgctca
     1261 agtgtatgac gttgaggcgg tagaagaggt cttccctgaa cgtccgctcc ctcaccatct
     1321 gtgcaaggtc cctgttcgtg gccgctatga tacggacatc cacgcggatg acttcctcgc
     1381 cgcccacacg cgtgaactcg cgttcctgga tgaccctgag gagcttcgcc tgcatggcga
     1441 gagacatctc gctcacctcg tccaggaaca gggttccccg gtgcgcctgc ttgaagctcc
     1501 cctccctgcg cctgagggcg ccggtgaagg cccccttctc gtggccgaac agctccgact
     1561 cgagcaggcc ctcggagatc gccgcgcagt tgatcttgat gaacgggccg tctttcctcg
     1621 aggagttgta gtggatcgcg ccggccacca tctccttgcc ggtccccgac tctcccgaga
     1681 cgagcacggt tgcgtccgat gccgaggcgc gctgcgcggt ctcgagaagc ctcaccatgg
     1741 cagggctcct gccgatgatg ttcgacgagt cgaagcgctc gcccaccagg ctcttgagga
     1801 tgcggttctc ctccttgagc gagctgtgct ccatggcctg ttccatcttg agccgcaggg
     1861 cgtcgaagtc gaggggtttc gtgaggtagt cgtaggcccc tttcttcatc gcttcgatag
     1921 cggtctccac ggacgagtag gcggtcatga tgatgaccgg gatcgccggg ttgatctcct
     1981 tgatggaagc gagggcctcg agtcccgaga ccttgaccat gcggatgtcc atgaggacga
     2041 ggtcgaaggg ggcgtccctg accgcgtcta tggcctcctg gccgtcagac gcctcgctca
     2101 ccttgaaatc ccatccccca aggagggtct tgagcatggt gcggtgcccg agatcgtcgt
     2161 cgaccacgag cactgccctg tcaggctggt ttcccatgtg tccacctctc aaggtccgag
     2221 cgtgacccgt accgtcgtcc cctggcccat gcggctggtc accttgatgt ccccgccgtg
     2281 ggcctcgacg atcttgtgga ccacggcgag gcccaacccg gtgcctgact gcttggtggt
     2341 gaagtacggg tcgaagatat gttcgagatg ttccggatgg atgccgcagc cggtgtcgct
     2401 cacctcgatc gtcacgggcg agtcgatgcc gttgcattcg agggcgacgc tgagcacacc
     2461 cccggaatcc atcgcctcca tggcgttgag gtagagattg agcatgacct gggctatctt
     2521 gtccgggtcg gccacgacgt gacaggggtt ctctcccagc ccggaccggt cgatggtgat
     2581 ggatctcctc gcagcctggt cctccaccgt ctcgagcgtc cgtctcacga tggagagcag
     2641 ggaacagcgc tccttcttca tgtccagggg cttggcgagg tccaggagct ggccgatcac
     2701 cctgttcagc ctctcaacct cgccgatcat gatgtcggcg atcctcgcat cctcgggcac
     2761 catgtggtag cgctccctga agtaggtggc gaaccccttg atcgagctca gggggttcct
     2821 gatctcgtgg gccaccccgg cggcgaggct c
//
LOCUS       DAOSCR010000364         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22882_length_2836_cov_4.438332, whole genome shotgun sequence.
ACCESSION   DAOSCR010000364 DAOSCR010000000
VERSION     DAOSCR010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22882_length_2836_cov_4.438332"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..553
                     /locus_tag="PLT69_08950"
     CDS             125..553
                     /locus_tag="PLT69_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOJ14603.1"
                     /translation="MAHDAIGIEALRAYFEKDYFAAFVGVEIVDTGPGTALVRLRVDQ
                     RHRNSLGMAHGGALFTLADMAFAVASHTRGLSAVAVNTTMTFMKAAYGDVLYARAREV
                     SRNRRLATYLVEVMDEPGEVLCLFQGTVYIKGDPVARPDR"
     gene            complement(775..1581)
                     /locus_tag="PLT69_08955"
     CDS             complement(775..1581)
                     /locus_tag="PLT69_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOJ14604.1"
                     /translation="MARNVRLVVLALAVLATASSAFAIPLGTNITINDLVNGSSSWYG
                     SAANTSVGREDQEVEVNCLTGQSWDLEAFFLDGYILSLVGGFDFVNGYGGYRAGDIFI
                     DINGDAKYGTANTSSGSGNSVVNNTFGYDFVIACDFSQMRYDVYQLFDGVSTVTVFYS
                     QNDESNPWVYNDGGTLVAFGDITYSSGLSDADAGYLLGGSHNVARFDLGFLQYFEGFN
                     GSFLAHTTLECGNDNLIGTGTAPVPEPATLLLLGSGLIGLASIGRRKRTR"
     gene            1972..>2836
                     /locus_tag="PLT69_08960"
     CDS             1972..>2836
                     /locus_tag="PLT69_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14605.1"
                     /translation="MYFFFSLPAAAVCLLGGAIILVRSGARSGRALACGLLAVSAVQA
                     CDSVFLLGRNQILAAQAASVFELVGAGCLVCAMIGMERGLASRGRLMRAASVGVTALC
                     AGYACILVFTRASFALFSPEGAIALDRLGRAHAVALLLVSTSGVWLVENILRSSTGRT
                     RTVFRFPAVGLVSILGASCLLAIHRLATSTLSVEVTALGSLMVLVGSSLLVFFSIRHR
                     LFELDVFVSRYVVYHSLTLLSMGLYLVLTGAAILGVQRLGLKSPVVVTCFIAFLALVV
                     FFVLSLSPEARA"
BASE COUNT          446 a          908 c          948 g          534 t
ORIGIN      
        1 gggcgtgcgc ctggcgaggg cgcacggggt cacgctcgcg tgccgcgcca aggacggtgc
       61 cttcaacctg tacgcccacc cggagcgcat ccggggcctg ggcgcgtccc gggggtgagc
      121 ggccgtggcg cacgatgcga tcgggatcga ggccctcagg gcatacttcg agaaggacta
      181 cttcgccgcg ttcgtggggg tggagatcgt ggataccggc cccggtaccg ccctcgtgag
      241 gctccgggtg gaccaacggc acagaaacag cctcggcatg gcccacggcg gcgccctctt
      301 cacgctcgcg gacatggcct tcgccgtggc ctcgcacacc cgcgggctct cggcggtggc
      361 cgtcaacacc accatgacct tcatgaaggc ggcctacggc gacgtgctct acgcaagggc
      421 acgggaggtc tcgaggaacc gcaggctcgc cacctacctc gtggaggtca tggacgaacc
      481 gggcgaggtc ctctgcctgt tccagggcac ggtctacatc aagggggacc cggttgcccg
      541 acccgaccgg tgacgttccc ccgcccgcag gcgtgatgtc cgggcacaag gcggcggcag
      601 tatggtcgta atgagcgcca tggctctctg agggatgtcc gggcacgagg cggcagctgc
      661 actcgtctcg tgacgttccc ccgcacgcag gcggcatcct tcccgccgga aaaaaacagg
      721 ggcccggctg agtgccgggc cccgcaggtg ccgtccttga agagatgagc cttgttaccg
      781 tgtcctcttc ctcctcccga tgctcgcaag cccgatcagg ccggagccca gaaggagcag
      841 tgtcgccggc tcgggcacgg gcgccgtgcc tgtgccgatg aggttgtcgt tgccgcactc
      901 gagcgtcgtg tgggcgagga agctgccgtt gaagccctcg aaatactgga ggaaccccag
      961 gtcgaaccgg gccacgttgt gggagccgcc caagaggtag ccggcatccg catcggaaag
     1021 tccgctagag taggtgatgt cgccgaaggc cacgagcgtt ccgccatcgt tgtacaccca
     1081 cgggttcgac tcgtcgttct gggagtagaa gaccgtgacc gtcgagacgc cgtcgaagag
     1141 ctggtagaca tcgtacctca tctgggagaa gtcgcaggcg atcacgaagt catagccgaa
     1201 ggtgttgtta acgacgctgt tgccagatcc gctgctggtg ttggccgtgc cgtatttcgc
     1261 gtcgccgttg atgtcgatga agatatcacc cgcgcggtag cccccatagc cgttcacgaa
     1321 gtcgaacccg cccacgaggc tcaggatgta gccgtcgagg aagaaggcct cgaggtccca
     1381 cgactgccct gtcaagcaat tcacctcgac ctcctggtcc tctcggccca cgctcgtgtt
     1441 ggcggcgctg ccgtaccagc tcgagctgcc gttgacgagg tcgttgatcg tgatgttcgt
     1501 gcccagggga atggcgaagg cggaagatgc cgttgcaagc accgccaggg ccaacacaac
     1561 aagtcgaacg tttcgtgcca tgatgcaagc ctccatgtga aaaaagtatt cagtcccccg
     1621 gggaaagcaa tgatcatgcc atggcaggaa aatcataaaa atcaaatggt tatgagatat
     1681 gacgggctgc cgggaatggg tgactgtaaa atccgccgac agcaccgccc cgtctggtcc
     1741 tgggtgcatg gggcagggat cgccttcggg cctggatcga gggtgcgcgc cgccttgagg
     1801 gcgggcggcc ttctcgaggt ctatcgacgg gcggtctcct gtaggcggca agctgcgtgc
     1861 agtgatcggc cgtgcgaggt tccggccgat cccgacaccc cgggggaaac ctgtaaatta
     1921 tttcgacacc gttgacgata tctggtagga aggggtcgag aggcgagtac catgtacttc
     1981 ttcttcagct tgcctgcagc cgcggtgtgc ctgctcgggg gcgcgatcat cctcgtgcgc
     2041 agcggagcga ggtccggcag ggccctcgcc tgcggcctcc tcgccgtctc cgccgtgcag
     2101 gcgtgcgact cggtgttcct gctcgggaga aaccagatcc ttgcggccca ggccgcctcc
     2161 gtcttcgagc tcgtgggcgc gggctgtctc gtctgcgcca tgatcgggat ggagcggggt
     2221 ctcgcgtcgc gcggccggct catgagggcc gcatccgtcg gcgtcacggc gctctgcgcg
     2281 ggctacgcgt gcatcctcgt gttcacgaga gcctctttcg cgctgttctc gccggagggc
     2341 gccatcgccc tggacaggct gggcagggcg catgccgtgg cgctgctcct cgtgtcgacc
     2401 agcggggtct ggctcgtcga gaacatcctg aggtcttcca cgggccggac gaggaccgtg
     2461 ttcaggttcc cggccgtcgg tctcgtctcg atcctcggcg catcctgcct gctcgccata
     2521 cacaggctcg ccacgagcac gctgagcgtg gaggtgaccg ccctgggctc gctcatggtc
     2581 ctcgtcgggt cctcgctgct cgtcttcttt tccatccgtc accggctctt cgagctggac
     2641 gtgttcgtct cccgctacgt ggtgtaccac tccctcaccc tcctcagcat ggggctctac
     2701 ctcgtgctca ccggggcggc gatcctcggt gtccagcggc tggggctgaa gtcgccggtg
     2761 gtggtcacgt gtttcatcgc ctttctcgcg ctggtggtgt tcttcgtgct ctcgctctcg
     2821 cccgaggcga gggctt
//
LOCUS       DAOSCR010000365         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_22999_length_2825_cov_2.718412, whole genome shotgun sequence.
ACCESSION   DAOSCR010000365 DAOSCR010000000
VERSION     DAOSCR010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22999_length_2825_cov_2.718412"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLT69_08965"
     CDS             <1..466
                     /locus_tag="PLT69_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ14606.1"
                     /translation="AAQTGHLVLSTIHANDSVGAVPRLKDLGMMDYMISSSLVCVIAQ
                     RLVRSLCPHCRQEYQSGEDEMRIFGIAPGTRLYAPVGCPRCIKTGYLGRTMVSEILVV
                     TKEIERLISTGAHPFEIRERALEEGMVSMVADGARKILSGKTSFKEAWRVLR"
     gene            466..1680
                     /locus_tag="PLT69_08970"
     CDS             466..1680
                     /locus_tag="PLT69_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ14607.1"
                     /translation="MLLTYSAMRKDGTVITSSAEFEDIEELVQTLSASDLTLVSYRRQ
                     TWPGIARIMESLQPSVGRMEIVEFCESLGSMIGSGLPLLDSLETIRDTVGSKRLKKAI
                     GEVISEISRGESLSGAFSHQGSVFPAILVFFSKIGEETGTIRDALTNTASYLRKIDAI
                     VSQVKKAFIYPSFVMASMGCVMIFWLFYVLPRLVTTFQDMNVRLPDITLSLVRVVGFV
                     QAYWYLTPVPLVLAAAGLFFVMKLDAARYVLTATAFRVPLVGDMLKSSMLTLLFSSLS
                     LMLRSGLTLTRSLDILLKLHGNPLVRKVITSIKHATASGSSLLESFESTGFFDAVTLK
                     MVSVGEKTGTLEGRLGYLADVYQEKTSRFVDAAGKMIEPMVMILSGGLFIFIVVSLIG
                     PVYDLMSQLGGG"
     gene            1682..2332
                     /locus_tag="PLT69_08975"
     CDS             1682..2332
                     /locus_tag="PLT69_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ14608.1"
                     /translation="MRPVHDTGRISGRHGAKSGFTLIEVAFVMVILGLLIGLGAQLLP
                     MLVKQNKLKDNRVLVKEAKTAVIGYALATGRLPYASANTGGNESAGRLNGYLPWATLG
                     IPGKDPYSTTLFYAVDSHLCSTTSAAQLKTRLADLISGTLTPDLFSDGTNIKAAFIVL
                     SAGEDFRVNPPNDDNNNTIVDIFDNNRFASPYEPFTASYDDVLEAVSLAYLHGLLP"
     gene            2417..>2825
                     /locus_tag="PLT69_08980"
     CDS             2417..>2825
                     /locus_tag="PLT69_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14609.1"
                     /translation="MRLWAARGKKGYAALLGDADHRVARYRIEGGACVLERVQVVDTQ
                     TLCKLATRHPGVRVCICARSVYSDIGEFSRVSEAAAKAHIKTTVDKIGLFREEYRISA
                     IKLEDTDDLKGKYSFLVLPLSNLSPLGMLDEDNA"
BASE COUNT          530 a          932 c          844 g          519 t
ORIGIN      
        1 agcggcccag acagggcacc tcgtcctgtc cacgatccac gcgaacgact cggtgggtgc
       61 cgtgccgaga ctcaaggatc tcgggatgat ggactacatg atctcctctt ccctcgtttg
      121 cgtgatcgcg caacgcctcg tgcgctcgct ctgcccccac tgcaggcagg aataccaatc
      181 tggcgaggac gagatgcgca tcttcggcat cgccccgggc accaggctct acgccccggt
      241 cggatgcccg aggtgcatca agacagggta cctgggcagg accatggtct ccgagatcct
      301 cgtggtgacc aaagagatag aacggctcat ttccacgggg gcccacccct tcgagatccg
      361 ggaacgggcc ctcgaggagg gcatggtgag catggtcgcc gacggcgcac gcaagatcct
      421 ctcgggcaag acgagcttca aggaggcgtg gagggtgttg cgctgatgct tctcacctac
      481 agcgccatgc ggaaggacgg caccgtgatc acgtcctcgg cggagttcga ggacatcgag
      541 gaactcgtcc agacgctttc cgcaagcgac ctcacgctcg tctcgtacag gcggcaaacc
      601 tggccgggta tcgccaggat catggaatcc ctgcagcctt ccgtgggaag gatggagatc
      661 gtcgagttct gcgagtccct gggctccatg atcgggagcg gtctccccct cttggactcc
      721 ctcgagacca tcagggacac ggtcggctcg aagaggctca agaaggcgat cggcgaggtg
      781 atctccgaga tctccagggg ggaaagcctc tcgggggcct tctcccacca gggctccgtc
      841 tttcccgcca tcctcgtgtt cttctccaag atcggcgagg aaacgggaac gatcagggat
      901 gcgctcacca acaccgcttc ctacctgagg aagatcgatg cgatcgtctc gcaggtgaag
      961 aaggccttca tctacccctc cttcgtcatg gcatccatgg gatgcgtgat gatcttctgg
     1021 ctcttctacg tgctcccgcg gctcgtcacg acgtttcagg acatgaacgt gcgcctgccc
     1081 gacatcacct tgtcgctggt gagggtcgtg ggcttcgtgc aggcatactg gtacctcacc
     1141 cctgtccccc ttgtcctcgc cgcagccggc ctgttcttcg tcatgaagct cgatgcagcg
     1201 aggtacgtgc tgaccgcgac ggccttcagg gtcccgctcg tcggcgacat gctcaagtcc
     1261 tccatgctca cgctcctctt ctcgagcctc tcgctcatgc tcaggtcggg gctcaccctc
     1321 acccggtccc tggacatcct gctcaagctt cacggcaatc ccctcgtcag gaaggtcatc
     1381 acctcgatca agcatgccac cgcctccggc agctcgctcc tcgaatcctt cgaatcgacg
     1441 gggttcttcg acgcggtgac gctcaagatg gtgagcgtgg gggagaagac gggcaccctc
     1501 gagggacgat tgggctacct tgccgacgtg taccaggaga agacctcccg cttcgtggat
     1561 gccgcgggca agatgataga acccatggtc atgatcctgt ccggcggcct cttcatcttc
     1621 atcgtcgtgt ccctcatcgg cccggtctac gacctcatgt cccagctcgg aggaggttga
     1681 catgcgacca gtgcacgaca ccgggaggat ttctgggcgg cacggcgcca agagcggctt
     1741 cactctgata gaggtggcct tcgtcatggt gatcctcggg ctgctcatcg ggctgggtgc
     1801 gcaactcctc cccatgctcg tcaagcagaa caagctcaag gacaaccgcg tcctcgtgaa
     1861 ggaggcgaag acggccgtca tcgggtatgc gctcgcgacc ggcaggctcc cctatgcgag
     1921 cgcgaacacg ggcggaaacg agagcgcagg caggctgaac gggtacctgc cgtgggccac
     1981 cctcggcata ccggggaagg acccctacag caccaccctg ttctacgccg tcgactccca
     2041 cctgtgctcg acgacctctg cggcccagct caagacccgc ctggcggacc tcatctcagg
     2101 gaccctcacc cccgacctct tcagcgacgg gacgaacatc aaggcggcgt tcatcgtgct
     2161 ctcggcgggc gaggacttca gggtgaaccc gccgaacgac gacaacaaca acaccatcgt
     2221 ggatatcttc gacaacaacc gcttcgcatc gccgtacgag ccgttcactg catcctacga
     2281 cgacgtcctc gaggcggtga gcctcgcgta tctccacggc ctcctcccct gagacccgcc
     2341 ggagaccccg accttatcgt caagaatggc tgggcgcctg ccgaaacgat gggaaacggc
     2401 ttcgggagaa gcagtaatgc gcctttgggc tgcgagggga aagaaagggt acgccgcgct
     2461 cctcggcgat gccgaccacc gtgtcgcgcg gtacaggatc gagggcgggg catgcgtgct
     2521 cgagcgcgtc caggtcgtgg acacccagac cctctgcaag ctcgccacga gacatcccgg
     2581 ggtgcgcgtc tgtatctgtg cgcggtcggt ctactccgac atcggagagt tctcgcgtgt
     2641 ctccgaggcc gcggccaagg cccacatcaa gaccaccgtc gacaagatcg gcctgttcag
     2701 ggaggaatac cggatctccg ccataaagct cgaggatacg gacgatctca aggggaagta
     2761 ttctttcctg gtcctgcccc tgtccaacct gagccctctc ggcatgctcg acgaggacaa
     2821 cgcgg
//
LOCUS       DAOSCR010000366         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23131_length_2813_cov_3.494561, whole genome shotgun sequence.
ACCESSION   DAOSCR010000366 DAOSCR010000000
VERSION     DAOSCR010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23131_length_2813_cov_3.494561"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLT69_08985"
     CDS             complement(<1..485)
                     /locus_tag="PLT69_08985"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ14610.1"
                     /translation="MLVIRSGEAAWRTTLEHLLDRMRTVPAEVDNTVRKIIDQVRVNG
                     DAALFEYTRMFDGFDPAREGLVIGPEAIQRAWSDAPAALKEALKLAARRIEAYHEHQK
                     ERSWFVAEGDGSITGQKVTPIDSVGIYIPGGANAFPSTMLMTVIPARIAGVGRIEVVS
                     PT"
     gene            complement(485..1750)
                     /gene="murA"
                     /locus_tag="PLT69_08990"
     CDS             complement(485..1750)
                     /gene="murA"
                     /locus_tag="PLT69_08990"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ14611.1"
                     /translation="MDKFVIRGGRRLSGEVVVSGSKNASLPVLCSSILTDKECTFTNV
                     PDLEDIKTMCKVLESLGVEVERPRPKTVLVRANGLNVFEASYDHVKKMRASILVLGPL
                     LARYGRAKVSLPGGCAIGVRPIDMHLKALARMGAEIELSHGYVVARASRLKGARIVFD
                     NTTVGGTENILMAAVLAEGTTVIEGAAQEPEVVDLAEALVAMGAKIDGIGERTLVVEG
                     VDELSGTNHRVIPDRIEAGTFMVAAGMTRGRITIQGARPDHVTAVIDKLMEMGIDIRS
                     DNDGDLVVDGDRDLEPASVETVPYPGFPTDMQAQVMALMCLARGVSSVTETIFEHRFM
                     HVPELMRMGAKLEEKGRTVIVRGIERFQGASVMATDLRASASLVLAGLNASGQTEVRR
                     IYHLDRGYEALEKKLNALGADISRERGGL"
     gene            complement(1743..2594)
                     /gene="prmC"
                     /locus_tag="PLT69_08995"
     CDS             complement(1743..2594)
                     /gene="prmC"
                     /locus_tag="PLT69_08995"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOJ14612.1"
                     /translation="MATCVARALASARAVLAGAGIESASLDAQVLLCRCLHATRERLV
                     ADPNRELTPGEESEFMAMVGRRARREPVAYIVGSKEFYGRDFAVNPEVLIPRPETELL
                     VELACTRAPRGGTVFEVGVGSGAVICSILAERRDLKGVANDVSMGAVKTALMNARALG
                     VADRLTLFRGRFLEGLAVGVDLVVANPPYVSDGDMRLLPEEVRLFEPEEALRGGPDGL
                     RVVKEIIFGAREALCPGGEILMEVGYGQKDAVEKLFLGCGGMTVTGWVRDLAGIERVV
                     IGKRCHG"
     gene            complement(2598..>2813)
                     /locus_tag="PLT69_09000"
     CDS             complement(2598..>2813)
                     /locus_tag="PLT69_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ14613.1"
                     /translation="REIATQRRMQVGTGDRSERIRTYNFPQGRVTDHRVNLTLHRLQQ
                     VLEGDIDEIVDALAAHEQAEVLRKTGS"
BASE COUNT          442 a         1021 c          822 g          528 t
ORIGIN      
        1 gtgggggaga ccacctcgat ccggcccaca ccggcgatcc tggccgggat cacggtcatg
       61 agcatggtcg aggggaaggc gttggccccg ccggggatgt agatcccgac actgtcgatg
      121 ggcgtcacct tctgccccgt gatcgagccg tcgccctcgg cgacgaacca cgaacgctcc
      181 ttctggtgct cgtggtacgc ctctatcctc cgggccgcaa gcttcagcgc ctccttcagg
      241 gcggcagggg cgtcagacca tgccctctgg atggcctccg gaccgatcac gagcccttcg
      301 cgagccgggt cgaacccgtc gaacatcctg gtgtactcga acagcgcggc atccccgttc
      361 acccggacct ggtctatgat cttccttacg gtattgtcca cctctgcggg tacggtcctc
      421 atgcggtcga gaaggtgctc gagggtcgtc ctccaggcgg cttccccact ccgtatgacg
      481 agcatcacaa tccgcccctt tcccggctga tatccgcccc cagggcattg agcttcttct
      541 cgagcgcctc gtaaccgcgg tccaggtggt atatcctcct tacctcggtc tggccggaag
      601 cgttgagccc tgccaggacc aaggacgcgg acgcgcggag gtccgtggcc atgacgctcg
      661 cgccctggaa tctctcaatg cctcgcacga tcacggtcct ccccttctcc tcgagcttcg
      721 ctcccatgcg catgagctcc ggcacgtgca tgaaccggtg ctcgaagatg gtctcggtga
      781 cgctcgaaac cccgcgcgcc aggcacatga gcgccatcac ctgcgcctgc atgtccgtcg
      841 ggaagcccgg gtaaggtacc gtttccacgc tagcgggctc gagatcgcgg tctccgtcga
      901 cgacgagatc gccgtcgttg tccgagcgta tgtcgatccc catctccatg agcttgtcga
      961 tcaccgcggt gacgtgatcc ggccgcgctc cttgtatggt gatccttcca cgggtcatcc
     1021 ctgcggcgac catgaacgtc ccggcctcga tcctgtccgg gatcacccgg tggttggtcc
     1081 ctgacagctc gtccacgcct tccaccacga gagtgcgctc tccgatccca tcgatcttcg
     1141 cgcccatggc aacgagcgcc tcggccaggt cgaccacctc gggctcctgc gcggcgcctt
     1201 ctatcacggt cgtgccctcg gcgagcaccg ccgccatgag gatgttttcc gtgccgccca
     1261 cggtggtgtt gtcgaacacg atacgggccc ccttgagccg ggaggcgcgt gccacgacgt
     1321 agccgtggga gagttctatc tcggcgccca tccgtgcgag cgccttcaag tgcatgtcga
     1381 tgggccgcac ccctatcgcg caccctcccg gaagggacac cttggccctg ccgtacctgg
     1441 cgagcagggg ccccagcacc aagatcgacg cccgcatctt cttcacgtgg tcgtaggagg
     1501 cctcgaacac gttcaggccg ttggcgcgca cgagaaccgt cttcggccga ggcctctcca
     1561 cttccacccc gaggctttcg agcaccttgc acatggtctt gatgtcctcg aggtcgggca
     1621 cgttggtgaa cgtgcactcc ttgtccgtaa ggatcgacga gcacaagacg gggagggacg
     1681 cgttcttgga ccccgatacg acgacctcac ccgagagcct cctcccgccc ctgatcacga
     1741 acttatccat ggcacctctt ccctatgacc accctttcta tgcccgcgag gtcgcgtacc
     1801 cagccggtca cggtcattcc cccgcacccg aggaacagct tctcgaccgc gtctttctgc
     1861 ccgtagccga cctccatcag gatttcccct cccggacaga gggcctcgcg tgcaccgaag
     1921 atgatctctt ttactacacg aaggccgtcc gggccaccac gaagagcctc ctcgggctcg
     1981 aagagcctca cctcctcggg caggaggcgc atgtccccgt ccgatacgta cggcggattg
     2041 gccaccacaa ggtccacgcc gacggcgagc ccctcgagaa aacgccccct aaagagcgta
     2101 agcctgtcgg cgacccccag cgccctcgcg ttcatgagcg ccgtcttgac cgcccccatg
     2161 ctcacgtcgt tcgcgacgcc tttgaggtcg cgcctttctg caaggatgga gcatatcacg
     2221 gcccctgatc ccacgcccac ctcgaacacg gtgcctcccc gtggcgccct cgtgcacgcg
     2281 agctcgacga ggagctccgt ctccggcctg gggatcagga cctccgggtt cacggcgaaa
     2341 tcccgtccat agaactcctt gctcccgacg atgtaggcta ccggttcccg cctcgcccgc
     2401 ctgccgacca tggccatgaa ttcgctctcc tctcccggcg tgagttcccg gttcgggtcg
     2461 gctacgaggc gttccctcgt ggcgtggagg caacggcaga ggagcacctg cgcgtcgagg
     2521 gaggccgatt cgatccccgc gccggccaga acggcgcgcg ccgaggccag agcccgggcg
     2581 acgcatgtgg ccatggttca gctcccggtt ttcctcaaga cctcggcctg ctcatgtgcc
     2641 gcgagggcat ccacgatctc gtcgatatcg ccctcgagaa cctgctgcag ccggtgcagc
     2701 gtgaggttga cgcggtggtc cgtcacgcgg ccctggggaa agttgtaggt tcgaatgcgc
     2761 tcgctgcggt cccccgtccc gacctgcatg cgcctctgcg tcgcgatctc gcg
//
LOCUS       DAOSCR010000367         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23135_length_2812_cov_3.447588, whole genome shotgun sequence.
ACCESSION   DAOSCR010000367 DAOSCR010000000
VERSION     DAOSCR010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23135_length_2812_cov_3.447588"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLT69_09005"
     CDS             complement(<1..323)
                     /locus_tag="PLT69_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ14614.1"
                     /translation="MSRPATGPPSPQAAIRRCPSCGSGDVQSLADRSVRCRKCSFVLF
                     FSVVSAVAALIEDDIGRVLLVERGRDPQKGALDLPGGFVDYGESAEDAIVREVSEELN
                     LEIVRG"
     gene            complement(363..1016)
                     /locus_tag="PLT69_09010"
     CDS             complement(363..1016)
                     /locus_tag="PLT69_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HOJ14615.1"
                     /translation="MKARLVSFLFVVAVVLPAALHAEEPWKLAKDEAGIKVYTRSVPG
                     SSANEFKGVADVDAPLDVVIEVFKDIPSYPKWYGFCKEIRQLKQEGEFHRVIYFVLET
                     MGPVKDRDMVVDVMEARDMKARKSTITMRAFKEDYVPRNPVYVRMTEMEGSCVLTQVG
                     DQTTNVVYTVKADPAGYIPAFISNMIQKEQPFLTLKGLKEMVKKDLYRERAAAAPKG"
     gene            1133..1816
                     /locus_tag="PLT69_09015"
     CDS             1133..1816
                     /locus_tag="PLT69_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOJ14616.1"
                     /translation="MCGRFIQIANPQRVRAYIGEAEVEPGVSESFRPSYNVAPTREVL
                     AVLNTRPPRIALLRWGLIPFWTKDGSQSKALINARAETLETRPSFREPFRRRRCMIFA
                     DGFYEWDKKSKPKTPYLVRMRDRQPFAMAGLWDEWTDRTTNESVRSCTIVTTEANGLV
                     STIHDRMPAILRPESYPLWLAVGASDPAALKGVLAPYPQDLMEAFAVSMRMNDPAFDS
                     PECMEPARG"
     gene            2294..2773
                     /locus_tag="PLT69_09020"
     CDS             2294..2773
                     /locus_tag="PLT69_09020"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ14617.1"
                     /translation="MDFGAREVKVVDHRQLTLQLKALFASQGLAVLSTSDHGQPYCNL
                     VAFSSSDDLRYLVFATTRATRKFSNIGSEPRVSMLIDNRSNKVSDFHEAMAVTATGIA
                     AEAMGPARDALLKVHLAKHPHLEDFVMSPTCALMRVEVDRYYVVSRFQHVMVLKLKE"
BASE COUNT          513 a          926 c          864 g          509 t
ORIGIN      
        1 ccccgcacga tctcgaggtt cagctcctcg gacacctccc tcacgatcgc gtcctcggcg
       61 gactccccgt agtccacgaa cccgccaggc aggtccagag cgcccttttg gggatcgcgg
      121 cccctctcaa cgaggagcac cctgcctatg tcgtcctcga tgagcgcggc gacggccgag
      181 accacgctga agaagagcac gaaggagcac ttcctgcacc gcaccgatcg gtcagccagg
      241 gactggacgt cgcccgaccc gcaggacggg cagcgcctga tggccgcctg gggcgagggg
      301 ggtcctgtgg ccggccttga catgcaaccc tcacgccttc tctcgtgtac gtgcgtccag
      361 ggtcaaccct tcggggcggc agcggcccgt tcgcggtaga ggtccttctt gaccatctct
      421 ttcagcccct tgagggtcag gaaaggctgt tccttctgga tcatgttcga tatgaaggcg
      481 ggtatgtagc ctgctgggtc ggccttgacg gtgtagacca cgttggtcgt ctggtccccc
      541 acctgggtga gcacgcacga gccttccatc tcggtcatgc ggacgtagac ggggttgcgg
      601 ggcacgtagt cctccttgaa ggccctcatc gtgatggtgg acttccgcgc cttcatgtcc
      661 ctcgcctcca tcacgtccac caccatgtcc ctgtccttga caggtcccat ggtctcgagc
      721 acgaagtaga tgaccctgtg gaactcgcct tcctgcttga gctgcctgat ctccttgcag
      781 aagccgtacc acttggggta ggacgggatg tccttgaaca cctcgatgac cacatccagc
      841 ggggcgtcga cgtcggccac gcccttgaac tcgttggccg aggatcccgg cacggagcgc
      901 gtgtagacct tgatcccggc ctcgtccttg gcgagtttcc acggttcctc ggcatggagt
      961 gccgccggca acaccaccgc gaccacaaac agaaaagaca ccaacctggc tttcacgctc
     1021 ccccctccga aagctgtcat tgttttctga gcgctgttca ttctatcagg agccgagccc
     1081 cctggtaaag agcatcgtgg atttccggac gcgtagggga tacaatgcac ccatgtgcgg
     1141 caggttcatc cagatcgcga acccccagag ggtgagggca tacatcggcg aggctgaggt
     1201 ggagccgggc gtcagcgagt ctttcaggcc gagctacaac gtggcgccca cgcgggaggt
     1261 gctggcggtc ctgaacacga gacctcccag gatcgccctc ctcaggtggg ggctgatccc
     1321 gttctggacg aaagacgggt cgcaatccaa ggcgctcata aacgccaggg ccgagaccct
     1381 cgagacgagg ccctccttca gggagccgtt caggaggcgc aggtgcatga tcttcgcgga
     1441 cggcttctac gagtgggaca aaaagagcaa gccgaagacg ccctacctcg tgcgcatgag
     1501 ggaccggcag cccttcgcca tggcgggtct ctgggacgaa tggacggaca ggaccacgaa
     1561 cgagtccgtg cgttcgtgca ccatcgtgac caccgaggcg aacggcctcg tctcgaccat
     1621 tcacgaccgg atgcccgcga tccttcggcc ggagtcctat cccctctggc tggccgtggg
     1681 ggcgtcagac cctgcagcgc tcaaaggggt gctcgccccc tacccccagg acctcatgga
     1741 ggccttcgcc gtctcgatgc gcatgaacga ccccgccttc gactcgccgg agtgcatgga
     1801 gccggcaaga gggtaggccc cccggggcga gtggggacga ccttgcgcaa ggcgcggggg
     1861 atgcggtgcc tgcacctagg ggcgaaccgt gcttcgactg ctcctcgcgc cctcccccga
     1921 tacgcgcgcg gcgatccgac ggccgggggc cgagaaaggt ccatccaggc gtgtccgacc
     1981 gcagaaggaa aagctggtgg acactttgct gctgtacatc aaaagagaaa agtctatccg
     2041 ggcgtgtcac agagcaagac accatcatct ggaagggtct tgtcatcgca tcgtcttgat
     2101 cgagcctcgg caggggcggt cccgagcgtg tccgggctct tcacgaatgc cccctttcag
     2161 ctcgagccga acgagtcttg acacatcccc cgggatgcat tgactgtccc tgaacggccg
     2221 ttatttttgg atacaaggcc gtacacgcgc cccggcgtcc tgtcggcgtg tttcccatca
     2281 ggaaggaggg gccatggatt tcggggccag agaggtcaag gtcgtggatc accggcagct
     2341 cacgctccaa ctcaaggcac tgttcgcgag ccaggggctc gccgtcctgt ccacgagcga
     2401 tcacggccag ccctactgca acctggtggc gttctcttcc tcggacgacc tgagatacct
     2461 cgtcttcgcc accacccggg ccacgcggaa attctcgaac atcggctcgg aaccgcgggt
     2521 ctccatgctc atcgacaacc ggagcaacaa ggtgtccgac ttccacgagg ccatggcggt
     2581 gactgccacc gggatcgccg ccgaggccat gggccctgcc agggacgcgc tgctcaaggt
     2641 ccacctggca aagcaccccc acctggagga cttcgtcatg tctcccacct gcgccctcat
     2701 gcgcgtggag gtggacaggt actacgtggt gagccggttc cagcacgtca tggtgctcaa
     2761 gctgaaggaa tgaacctcgt catcccattg caggccgtcg ctaaagcggg tg
//
LOCUS       DAOSCR010000368         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23137_length_2812_cov_3.382662, whole genome shotgun sequence.
ACCESSION   DAOSCR010000368 DAOSCR010000000
VERSION     DAOSCR010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23137_length_2812_cov_3.382662"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLT69_09025"
     CDS             complement(<1..521)
                     /locus_tag="PLT69_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14618.1"
                     /translation="MVTKITDGITGFIWTDYRQNNCNTYLIDAGARILVDPGHSHLFD
                     HVEKGLAALGINRRSIDLVVVTHAHPDHMESALLFESPTMKTFPREDFLYIKRLAGDG
                     YPIPEPDFFLVEGDLEVGNTRLQVIHTPGHTPGSVCLFWPEKGALFCGDVIFVNGIGR
                     TDLPGSSAAALKE"
     gene            complement(677..1270)
                     /locus_tag="PLT69_09030"
     CDS             complement(677..1270)
                     /locus_tag="PLT69_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOJ14619.1"
                     /translation="MERHTPTREEALRLLHEYVGAENLRCHAYAVEAVMRHMARKRGE
                     DEDKWGIVGLVHDLDYERHPDQHCGKVREILEERGWPEEYIRAVVSHGYGLCSDVEPK
                     SPMERALYAIDELTGLVSACALVRPSRSVMDLTVGSVKKKWKSSGFAAGVDRSIIERG
                     AQMLGVSIEELIEDTIAGMREVAEDIGLAGPPADAAP"
     gene            complement(2124..>2812)
                     /locus_tag="PLT69_09035"
     CDS             complement(2124..>2812)
                     /locus_tag="PLT69_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ14620.1"
                     /translation="LPRDYRVVAFDLPGHGSSFRDWEETYTISFMADTLGQAFDALKL
                     ERFHLVGNSMGGWVGTIYTSRNPDRVMTLALFDSAGTESPVPSDRQKALEQGRNVLVP
                     ETEEEFHELLGYAFHDKPFIPWPIGSVLARRAVQDAPFKRKIFADVNGARRDVREVLP
                     EVNLPVLILWGAHDRITHVSAAQVFERYLPKSETVIMQECGHMPMIEKPRETAGRYVD
                     FLRRHSTGTP"
BASE COUNT          469 a          906 c          861 g          576 t
ORIGIN      
        1 ctctccttga gtgccgcggc gctcgatccc ggcaggtccg tacggccgat cccgttcacg
       61 aagatcacat caccgcagaa gagggcccct ttttccggcc agaacaggca cacggagccc
      121 ggggtgtgcc cgggcgtgtg tatgacctga agccttgtgt tcccgacctc gaggtccccc
      181 tcgacgagga agaaatccgg ctcggggatc gggtatccgt cgcctgcgag cctcttgatg
      241 tagaggaagt cttcccttgg gaacgtcttc atggtcgggc tctcgaagag cagtgccgac
      301 tccatgtgat ccgggtgggc gtgcgtgacc accacgaggt ctatcgaccg gcggtttatc
      361 cccagggccg cgagtccctt ctccacgtgg tcgaacaggt ggctgtgccc cgggtccacg
      421 aggatccggg cccctgcgtc tatgaggtag gtgttgcagt tgttctggcg gtagtccgtc
      481 cagatgaatc ccgtgatacc atccgttatc ttcgtgacca tgtgatcgac ctctctcgcc
      541 tcttcgaacg ggccgtttcc ctcgaccggg gcgaagagcg ccgacggctc gaaaaaggtc
      601 ccgcctgcgg cgcttccacc ccggggtctc ccgggtgacc ctctgcttct tgggtagggt
      661 cttccaggcg ccccggtcaa ggtgcggcgt cagcgggggg acctgccagg cctatgtctt
      721 cggcgacttc ccgcatgccc gcaatggtat cctcgatgag ctcctctatc gacacaccga
      781 gcatctgcgc cccccgctcg atgatcgaac ggtcgacccc ggcggcgaac ccgctcgatt
      841 tccacttttt cttgaccgag ccgaccgtga ggtccatcac gctcctcgag ggcctgacga
      901 gggcgcatgc gctgacgagc cccgtgagct cgtctatggc gtagagggcc cgttccatgg
      961 ggctcttggg ttcgacgtcc gagcacaggc cgtacccgtg ggagacgacg gccctgatat
     1021 actcctcggg ccaccccctc tcctccagga tctcgcggac cttcccgcag tgctggtccg
     1081 ggtggcgctc gtagtccagg tcgtgcacga gccccacgat cccccacttg tcctcgtcct
     1141 cacccctctt gcgggccatg tgacgcatca cggcctccac ggcatacgca tggcatcgga
     1201 ggttctccgc cccgacgtac tcgtggagga gccggagcgc ctcttcgcgt gtcggtgtgt
     1261 gtctctccat gaactccctc cgcgttcgag tgcatgaact atcctccttt tcggggcggg
     1321 atgcaacacc gtcccaaggc gctcgtggcg taaccgtccc gccccgctgc ccaggaaccc
     1381 ggctcgccga ggtggagagc cgctttatga caggtcgcgt gacaggccct gaagcgaagc
     1441 tgccgtgacc cgggacgccg tttggagaga atccgccaag gggtgtgtgt gcccggaaac
     1501 cccacccgac ggagagagat ttcccttttt ccgagctcat agtggaaaag aattacacaa
     1561 gatccttgca aagttcccag gtttcgcacg ggatattgac gcaatataat gaatatattc
     1621 aataagtgtc tatactatac tatggcatgg ctcttgaata tcgatacaca ctgttgtcgc
     1681 acccggacgt tgcggccggg gcggttacga agagtcggtc ggtagagacg ggacccctca
     1741 ggatgctcat atcctattcc tcctcaggcc gcatccagca atggatgcgg cctgctccct
     1801 cacttctgga gatacagctt gacccctgtg ctggcgcctg gacctgcggg catgacccgt
     1861 tgatcgaggg tcggggtcat ttaagctcac cgcatcaagg gatctcttgc cgaacctgca
     1921 tggagatcca caggcggcgt cccacgcacc cacgcattga ttgctccatt agtagaaagc
     1981 catccgctgt gccctgggat tgaggaccca aagcgaactc gcggctactc gcgtgatgcg
     2041 gaaagccgct tcatcgcgtc cggcttccca ggcgggcgcc acggtgctgc cgcagcctgt
     2101 gcccctttgg agcccctcgt tcttcacggg gtgcctgttg agtgcctccg gagaaagtcc
     2161 acgtagcggc cggccgtttc gcggggcttc tcgatcatcg gcatgtgccc gcactcctgc
     2221 atgatcacgg tctcgctctt cggcaggtag cgctcgaaga cctgcgcggc ggagacgtgg
     2281 gttatcctgt cgtgggcccc ccacaggatg aggaccggca ggttcacctc cggcaggacc
     2341 tccctgacgt cgcgcctggc cccattgacg tccgcgaaga tctttctctt gaagggggcg
     2401 tcttgtaccg cccttcgagc gaggaccgac ccgatgggcc acgggatgaa cggcttgtcg
     2461 tggaatgcgt agccgagaag ttcgtggaac tcctcctctg tctcgggcac gaggacgttt
     2521 ctcccctgct cgagggcctt ctgcctgtcg ctcggcacgg gggactcggt accggcggag
     2581 tcgaagaggg cgagcgtcat gaccctgtca gggtttctcg atgtgtagat cgtgcccacc
     2641 catccgccca tggagttgcc gacgaggtgg aaccgctcga gcttcagcgc atcgaatgcc
     2701 tggccgagcg tgtccgccat gaaggagatg gtgtaggtct cctcccagtc cctgaagctt
     2761 gaaccgtggc cgggcaggtc gaaggcgacg acgcggtagt cgcggggaag gt
//
LOCUS       DAOSCR010000369         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23232_length_2804_cov_3.626410, whole genome shotgun sequence.
ACCESSION   DAOSCR010000369 DAOSCR010000000
VERSION     DAOSCR010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23232_length_2804_cov_3.626410"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLT69_09040"
     CDS             <1..247
                     /locus_tag="PLT69_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14621.1"
                     /translation="VIGTSAQIEPAASLPIVAKGMHSMFRGMGAGFVKVDAHVIEINR
                     EPTPLTGVVSDFLIQGSAGEILSAVLNRVEKKRRSSA"
     gene            244..1056
                     /locus_tag="PLT69_09045"
     CDS             244..1056
                     /locus_tag="PLT69_09045"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14622.1"
                     /translation="MITGRTRVYTILARPSVHVTAPMVYNHLFSRMGLDMVYISHDIP
                     PEAVDATVASFASWENLGGFNVTVPHKESVARLVNPACPVAGAIGVVNTVVRRENGAL
                     EGYNTDGEGALKALGDVAGARCLILGAGGAARAVAYALAGAGASRISVMNRTPETARR
                     LCSLVGHACVHVYDGEPLDGFDILVQATPVADRVPFDLDLSRLRPGARVLETVMRRTL
                     LAQRVKDLGLELVSGYAMLYHQTPRNFELLTGIPLPERLLEEAFALVGFRLP"
     gene            1053..1217
                     /locus_tag="PLT69_09050"
     CDS             1053..1217
                     /locus_tag="PLT69_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14623.1"
                     /translation="MRAYIVCPHRKSNPKVAVEVCRACRRRKTCRRYHAYLNPPLFSE
                     HHPPVGSTPS"
     gene            1472..1972
                     /gene="def"
                     /locus_tag="PLT69_09055"
     CDS             1472..1972
                     /gene="def"
                     /locus_tag="PLT69_09055"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ14624.1"
                     /translation="MQPRPIITYPDPVLKARASEIRSIDALVVTLAEDMRETMYLAPG
                     VGLAAPQVGVSSRLIVVDPTAGKEPGRLIVMLNPIIVESEGSLTDSEMCLSVPEVSVN
                     VKRAQRILVRGMDLKGREVSVEAEGYLARIFQHEIDHLDGKVILDYASSLKRALYLKK
                     KKKGLV"
     gene            1969..>2804
                     /gene="fmt"
                     /locus_tag="PLT69_09060"
     CDS             1969..>2804
                     /gene="fmt"
                     /locus_tag="PLT69_09060"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ14625.1"
                     /translation="MRIAFMGTPMFAVEALKALVERTTHEIVFVVTQPDKPQGRGLRL
                     VSPPVKILANRHNIPVHQPASLKANEQMRMVFASAGLDIVVVVAYGKMIPADWLGLPR
                     FGFVNVHASLLPEFRGASPINRAILAGKTVTGVSIMRIEEEMDAGPVYARVSTPIGED
                     EDASSLADRLSVLGAEKLLEVLPLIASGALTAEPQDHARATYAPPLRREEGEIDWTMS
                     ARAICTMVRGLVPWPCAYTFLDGKMLKILKARWEKAAPGEPPGTMLRQKRGVCIACAD
                     GFV"
BASE COUNT          532 a          912 c          913 g          447 t
ORIGIN      
        1 cgtgatcggg acctccgccc agatcgagcc ggccgcatcc ctccccatag tcgccaaggg
       61 catgcattcg atgttcaggg gcatgggtgc agggttcgtg aaggtggatg cccacgttat
      121 cgagatcaac agggagccca cccccctcac cggcgtggtc tcggatttcc tgatccaggg
      181 cagcgcaggc gagatcctct cggccgtctt gaacagggtg gagaagaaga ggcggtcgag
      241 cgcatgatca cgggtcgcac ccgggtctac accatcctcg cgaggccgtc ggtccacgtc
      301 acggcgccta tggtgtacaa ccacctgttc tcccgcatgg gcctcgacat ggtctacatc
      361 tcccacgaca tcccccccga ggccgtggac gccaccgtgg catcgttcgc ctcatgggag
      421 aacctgggcg gtttcaacgt gaccgtaccg cacaaggagt ccgtggcaag gctggtgaac
      481 cctgcatgcc cggtggcggg ggccatcggc gtggtgaaca cggtggtgcg cagggagaac
      541 ggcgcactcg aggggtacaa caccgacggc gagggggcgc tgaaggcgct cggggacgtg
      601 gccggggcaa ggtgcctcat cctgggggcc gggggggcgg cccgcgccgt cgcgtatgca
      661 ctggccgggg caggggcatc ccgcatctcg gtcatgaacc ggacgcccga gacggcccgg
      721 agactgtgtt cgctcgtggg acacgcgtgc gtgcacgtgt atgacggcga gcccttggac
      781 ggcttcgaca tcctcgtgca ggccaccccg gtcgcagacc gtgtcccctt cgacctcgat
      841 ctttcccgcc ttcgacccgg agcgcgcgtg ttggagaccg tcatgcggcg gactcttctc
      901 gcgcaaaggg tcaaggacct cggcctcgaa ctcgtcagcg ggtatgccat gctctaccac
      961 cagacaccga ggaacttcga gctgttgacc gggatccccc tccccgagag gctgctcgaa
     1021 gaggcgttcg ccctcgtcgg gttcaggctg ccgtgagagc gtacatcgtc tgcccgcacc
     1081 gcaagtcgaa cccgaaggtg gcggtggaag tctgcagggc gtgcaggcgc aggaagacct
     1141 gcaggcgcta ccacgcgtat ctgaaccccc cgctgttttc ggaacatcac ccccccgtcg
     1201 gctccacccc atcctgaccg taggggaatt ccacccggca tgccacgttc tcaggccgtg
     1261 gtccgccacg cggcattccc ccttcctggc ccagggataa ccctgtttcc tcgagagaac
     1321 cccgtcctcg ccgcgacgcc tcgtacggac ccacgcagga ccggaaacgt accagccgcc
     1381 gccgatcccc accttttgcc cgcccccatg gaaccaccgg gaaacccact tccttgacag
     1441 cccaaacgag agacactatg gtatctgcac catgcagcca cggcccatca tcacctaccc
     1501 ggacccggtc ctcaaggcgc gcgcctcgga aatccgatcc atagacgccc tcgtcgtcac
     1561 cctcgctgaa gacatgaggg agacgatgta cctcgctccc ggagtggggc ttgcggcacc
     1621 ccaggtgggg gtgtcgagca ggctcatcgt cgtggacccg acggccggca aggaacccgg
     1681 gaggctgatc gtgatgctca accccatcat cgtggagagc gagggttccc tgacggacag
     1741 cgagatgtgc ctgagcgtcc ccgaggtgtc ggtgaacgta aagcgcgccc agaggatact
     1801 cgtcaggggg atggacctca agggcaggga ggtttccgtg gaagcggaag gatacctggc
     1861 gcgcatcttc cagcacgaga tcgatcactt ggacggcaag gtgatcctcg actacgcatc
     1921 ctcgctcaag agggccctct acctgaagaa aaagaagaag ggccttgtat gagaatcgca
     1981 ttcatgggca cccccatgtt tgcagtggag gccctcaaag ccctcgtcga aaggaccacg
     2041 cacgagatcg tgttcgtggt gacgcagccc gacaagccgc agggccgggg cttgaggctt
     2101 gtgagcccgc ctgtgaagat cctcgcgaac aggcacaaca tacccgtcca ccagccggcg
     2161 agcctgaaag cgaacgaaca gatgcgcatg gtgttcgcct cggcggggct ggacatcgtc
     2221 gtggtggtgg cgtacggaaa aatgatcccg gcggactggc tggggcttcc gaggttcggg
     2281 ttcgtgaacg tccacgcctc gctcctgccg gagttcaggg gcgcatcacc cataaaccgt
     2341 gcgatcctgg cggggaagac ggtgacgggc gtcagcatca tgcggatcga ggaggagatg
     2401 gacgcgggcc ccgtctacgc cagggtctcc acgcccatag gggaagacga agacgcctcg
     2461 agcctggccg acaggctcag cgtgctcggg gccgaaaagc tgctcgaggt gctccccctc
     2521 atagcatccg gggcattgac cgccgagccg caggatcacg caagggccac ctacgccccg
     2581 cccttgagga gggaggaagg ggagatcgac tggaccatga gcgcaagggc gatctgcacc
     2641 atggtgaggg ggctcgtccc ctggccgtgc gcatacacct ttctggacgg caagatgctg
     2701 aagatactca aggccaggtg ggaaaaggcc gcccccggcg aaccgcccgg gacgatgctc
     2761 cggcagaaga ggggggtctg catagcctgc gcagacggct tcgt
//
LOCUS       DAOSCR010000370         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23362_length_2793_cov_3.121256, whole genome shotgun sequence.
ACCESSION   DAOSCR010000370 DAOSCR010000000
VERSION     DAOSCR010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23362_length_2793_cov_3.121256"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..942
                     /locus_tag="PLT69_09065"
     CDS             82..942
                     /locus_tag="PLT69_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ14626.1"
                     /translation="MNRGVFRFISVGSTVSWIVLFVVIPYLFVVLFSTVSYEGLISGV
                     IDLSLDGFREICSSVFLEVCISSFSLALGATVLTLLVAYPYAYILTRLPEAVKRVMLL
                     LMIIPFWTSSLVRTYALIILLKANGIVNSVLLYAGLIDEPLSMLYTQWSVFAGLVYTL
                     LPFMVLPIYSSLQKLDWRLVEAARDLGATPFSVFARVVLPLTMPGLIAGSTMVFLPAL
                     GLFYIPDLLGGARSMLIGNYIKNQFLVAGNWSAGSAASVVLTLLMCLCIYGYFLSARK
                     ARTESMGGMP"
     gene            939..1706
                     /gene="potC"
                     /locus_tag="PLT69_09070"
     CDS             939..1706
                     /gene="potC"
                     /locus_tag="PLT69_09070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HOJ14627.1"
                     /translation="MSRVFRWVYLGLIYAFLYVPIAVLVVYSFNSSKYSTNWQGFTLK
                     WYGQLVSDSPLREAFVNSLVIAFASSVCATILGTLAAFAFYRYRFVGRKVLYTFIYIV
                     MMSPDIVMGISLLMFFVTLGLATGALTLLIAHITFCLPFVVVTVYSRISGLDRSIIDV
                     AKDLGANEMQVFRHVVMPLLMPAIIAGWLLSFTLSLDDVVVSFFTTGPSFDILPLRIY
                     SMVRLGIKPEINALSTVMFALSLVTVTVAHLVTKEKT"
     gene            1703..2734
                     /locus_tag="PLT69_09075"
     CDS             1703..2734
                     /locus_tag="PLT69_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ14628.1"
                     /translation="MMKRLAFLVALVLLPAALFGAARQVYVFNWTEYIDPDVLKQFEA
                     ETGIKVRYATYESNEAMFAKLKLVKKGGYDVAFPSTYYVERMRKHGLVTRLDKSKLPN
                     LRHLNPTLMDKAYDPGNAYSVPYMWGSSAIGVNRKAVDPKTITSWNDLWNPRYKGKVL
                     LMDDVREVFAMALRSMGYSGNTTDPVQIRLAYEKLLKLKPSVKVFNSDSPAQPFLNKE
                     VIIGQIWGGAVYQANQESPDLVLVYPKEGAIFWVDNMVIPSSARDVDAAHRFIDFMCR
                     PDVAKKNCEFIGYATANLTAQNMLSPKTKNHKAVYPGDETVKKGEFQADVGDAIRIYE
                     KYWEMLKTR"
BASE COUNT          505 a          911 c          805 g          572 t
ORIGIN      
        1 ggaccaggag aacgtcatct atcgcgaagg ccagcgtgtc cacgtcaact ggctctacgg
       61 gtgggaagtc gtgctgccgt catgaacagg ggggtcttca ggttcatctc ggtggggtcc
      121 accgtatcct ggatcgtcct gttcgtcgtg atcccgtatc tcttcgtcgt cctcttcagc
      181 acggtctcct acgagggcct gatctcggga gtcatcgatc tcagcctcga cgggttcagg
      241 gagatatgct cgagcgtgtt tctcgaggtg tgcatctcct cgttctcctt ggcgctgggc
      301 gccacggtgc tcaccctgct cgtcgcctac ccgtacgcgt acatcctcac ccgtctcccc
      361 gaggccgtaa agagggtgat gctcctgctc atgatcatcc cgttctggac atcgtcgctg
      421 gtgcggacct acgccctcat catcctcctg aaggctaacg gcatcgtcaa cagcgtgctc
      481 ctgtacgcag ggctcatcga cgagccgctg tccatgctgt acacacaatg gtcggtgttc
      541 gcgggcctcg tctacacgct tctgccgttc atggtcctgc ccatctactc ctcgctccag
      601 aaactcgact ggcgcctcgt cgaggcggcc agggacttgg gcgccacgcc gttctcggtg
      661 ttcgcgcgcg tcgtgctccc cctgaccatg cccggcctca tagccggcag caccatggtc
      721 tttctgcccg cgctcgggct cttctacata cccgatctcc tcggcggcgc gaggtcgatg
      781 ctcataggga actacatcaa gaaccagttc ctcgtggcag gcaactggag cgctgggtcc
      841 gcagcgagcg tggtgctcac gctcctcatg tgcctctgca tctacggcta cttcctgagc
      901 gccaggaagg cccggaccga gagcatgggg gggatgccgt gagccgcgtc ttccgctggg
      961 tgtacctcgg cctgatctac gccttcctct acgtgcccat agctgtgctc gtggtctact
     1021 cgttcaactc atccaagtac tccaccaact ggcagggctt cacgctcaag tggtacggtc
     1081 agctcgtgtc cgactccccc ctcagggagg ccttcgtcaa ctccctcgtc atcgcttttg
     1141 cttccagcgt gtgcgcgacc atcctcggca cgctcgcggc gttcgcgttc taccggtacc
     1201 gcttcgtggg gcgcaaggtg ctctacacct tcatttatat cgtcatgatg agcccggaca
     1261 tcgtcatggg tatctcgctc ctcatgttct tcgtgaccct gggcctggcc accggcgccc
     1321 tcacgctcct gatcgcccac atcaccttct gtctcccgtt cgtggtggtc acggtgtact
     1381 cgaggatcag cgggctcgac aggtccatca tagacgtggc caaggacctc ggcgcgaacg
     1441 agatgcaggt cttccgccac gtggtgatgc ccctgctcat gcccgccatc atagcggggt
     1501 ggctcctcag cttcaccctg tccctcgatg acgtcgtggt gagtttcttc accaccgggc
     1561 cgtccttcga catactgccg ctcaggatct attccatggt caggctcggg ataaaacccg
     1621 agataaacgc cctgtccacg gtgatgttcg ccctctcgct cgtcaccgtg acggtggcgc
     1681 acctcgtgac aaaggagaag acatgatgaa gaggctcgcc ttcctggtcg ccctggtatt
     1741 gctgcctgca gccctgttcg gcgccgcccg ccaggtctac gtcttcaact ggaccgagta
     1801 catcgacccc gacgtgctca aacagttcga ggcggagacg gggatcaagg tcaggtacgc
     1861 cacctacgag agcaacgagg ccatgttcgc gaagctcaag ctcgtgaaga agggcggata
     1921 cgacgtcgcc ttcccctcga cgtactacgt tgagcgcatg cgcaaacatg gtctcgttac
     1981 gaggctcgac aagtccaagc ttcccaacct caggcacctc aaccctacgc tcatggacaa
     2041 ggcgtacgac cccggcaacg cctacagcgt cccgtacatg tggggatcct cggccatagg
     2101 ggtcaacagg aaggcggtgg atccgaagac gataacgagc tggaacgacc tgtggaaccc
     2161 caggtacaag ggcaaggtcc tcctcatgga cgacgtgagg gaggtcttcg cgatggcgct
     2221 gaggtccatg ggatactcgg gcaacaccac ggaccccgtc cagatacggc tcgcctacga
     2281 gaagctcctc aagctcaagc ccagcgtgaa ggtcttcaac tccgactccc cggcgcaacc
     2341 gttcttgaac aaggaggtga tcatcgggca gatctggggt ggggccgtct accaggcgaa
     2401 ccaggaatcc ccggacctcg tgctcgtcta ccccaaggag ggggcgatct tctgggtcga
     2461 caacatggtg atcccctcct cggccaggga cgtcgacgcg gcgcaccgct tcatagactt
     2521 catgtgcagg ccggacgtgg ccaagaagaa ctgcgagttc atcgggtacg ccacagcgaa
     2581 cctgaccgcc cagaacatgc tctcgccgaa gacgaagaac cacaaggcgg tctacccggg
     2641 agacgagacg gtcaaaaagg gcgagttcca ggcggacgtg ggggatgcga taaggatcta
     2701 cgagaaatac tgggaaatgc tcaagacccg ctgatccggc ccgttcgctg gagtgtgcgt
     2761 gaggccgtgc tcccggcccg gggcgtcttt ccc
//
LOCUS       DAOSCR010000371         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23567_length_2774_cov_3.598750, whole genome shotgun sequence.
ACCESSION   DAOSCR010000371 DAOSCR010000000
VERSION     DAOSCR010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23567_length_2774_cov_3.598750"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PLT69_09080"
     CDS             complement(<1..1547)
                     /locus_tag="PLT69_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(492..494),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ14629.1"
                     /translation="MVITIDGRECTFTPGSTVYEVARDNGIAIPVLCHHEQLKPVGAC
                     RVCVVEVQGARSLVTSCAMPAENGMVVNTKSERVLKARRLSVELLLTQGHHNCLVCEQ
                     SGDCVLQSLAYELGIDAPRFSEPEGFRPVEQGNEMIVRDMNKCILCGLCVRACNEIQV
                     NQVLDFSGRGPTADVGPAFHLAYEDSTCVFCGECVRLCPVGALYEKQGRFKSRAGEAR
                     KVRTTCEYCGVGCQMELNVKDGRVVKVTTPRWNAPAPNRGSLCIKGRFGFDFLDHPDR
                     ITKPLIRIDGELKEASWDEAIAYVADRLSSIRDTFGPDAIAGLSSARCTNEENYLFQK
                     FMRAVIGTNNVDHCARLUHSSTVAGLAIAFGSGAMTNSIADLEESKCILVTGSNTTEN
                     HPVISTFIKRAVTQRGAKLILADPRRIDLARFATVWLRQKPGTDIAWINGLMNVIVTE
                     NLHDQAFIDERTENFDALKEVLKKYTPDYVERVTGIPAEDLKKAARLYATSGASAIVY
                     AMGITQH"
     gene            complement(1733..>2774)
                     /gene="hydF"
                     /locus_tag="PLT69_09085"
     CDS             complement(1733..>2774)
                     /gene="hydF"
                     /locus_tag="PLT69_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOJ14630.1"
                     /translation="DDTGELGTMRVKKTLAVLGKVNVALLVTTAQGLDEDDRDLMRRL
                     SGRGIVTIVVFNKADVERPGREVVDTLDRTGVRHVTVSSLTGEGIDRARQAIVDAASS
                     LRPEPPTIVGDLIGHGDCVLLIVPIDLGAPRGRLILPQVQVIRDILDNDAVAVVAKER
                     EIEYVMRGLARPPSLAVCDSQVVLKVSGDLPPDVRLTTFSILFSRLKGDLATFVEGVR
                     AIEGLADGDRVLILEACTHHPLPDDIGRVKIPRWLRQYTGRDLSFDVHAGPVLDRDVS
                     SYKLAVHCGGCMINRAEMLNRIGMLKDAGVPVTNYGVAISYVHGVLDRVLQPFPYERS
                     LLARVEEGGTKT"
BASE COUNT          454 a          918 c          850 g          552 t
ORIGIN      
        1 atgtgctggg tgatgcccat ggcgtacacg attgccgatg cgccggaggt ggcatagagt
       61 cgcgcggcct tcttcaggtc ctcggcaggg atccccgtga cccgctccac gtagtccggg
      121 gtgtacttct tgagcacctc cttgagggcg tcgaagttct cggtgcgctc gtctatgaag
      181 gcctggtcgt gaaggttctc ggtgacgatc acgttcatca acccgttgat ccaggcgatg
      241 tccgtccccg gcttctgtct cagccaaacg gtcgcgaacc tggcgaggtc gatcctccgc
      301 gggtcggcaa ggatcagctt cgccccgcgc tgggtcaccg ccctcttgat gaacgtggag
      361 atcaccggat ggttctccgt ggtgttgctg ccggtcacga ggatgcactt cgactcctcg
      421 aggtcggcga tagagttcgt catggccccg gaaccgaatg cgatggccag accggccacc
      481 gtggagctgt gtcagagccg ggcgcagtgg tccacgttgt tggtgccgat caccgcgcgc
      541 atgaatttct ggaagaggta gttctcctcg ttcgtgcacc gggccgagga gagaccggcg
      601 atagcgtccg ggccgaaggt gtcccggatg gacgacagcc tgtcggccac gtacgcgatg
      661 gcctcgtccc agctcgcctc cttgagttcg ccgtcgatgc ggatcagcgg tttcgtgatc
      721 cggtcgggat ggtcgagaaa gtcgaacccg aacctgccct tgatgcagag gctgcccctg
      781 ttgggtgcgg gcgcgttcca cctgggcgtc gtcaccttga ccacgcggcc gtccttcacg
      841 ttgagctcca tctggcagcc cacaccgcag tactcgcacg tggtgcgcac cttgcgcgcc
      901 tctccggccc tgctcttgaa acgcccctgc ttctcgtaga gcgcacccac gggacacagc
      961 ctcacgcact cgccgcagaa cacgcaggtg gagtcctcgt aggccaggtg gaaggcgggg
     1021 cccacgtcgg ccgtggggcc gcggcccgag aagtcgagca cctggtttac ctggatctcg
     1081 ttgcaggccc tcacgcacaa cccgcacagg atgcacttgt tcatgtcgcg cacgatcatc
     1141 tcgttgccct gctccacggg cctgaagccc tcgggctcgc tgaacctggg tgcgtctatg
     1201 ccgagttcat acgccaggct ctggagcacg cagtcgccgc tctgttcgca cacgagacag
     1261 ttgtgatgac cctgggtgag caggagctcc acggacaggc gcctggcctt caacaccctc
     1321 tcgctcttcg tgttcaccac catgccgttc tctgcaggca tcgcgcacga tgtgacgagc
     1381 gagcgcgcac cctgcacctc caccacgcac accctgcatg cgcccacggg cttgagctgc
     1441 tcgtggtggc agagcaccgg gatggcgatg ccgttgtccc tggccacctc gtacacggtg
     1501 cttccaggcg tgaatgtgca ctcccttcca tcgatggtta tgaccatacc tttcctccca
     1561 cgatatcgat tgccatggac actgaagaat acataccgca tcgcccgggg tctcgcggac
     1621 gtgccgagcc atcatggtgg ggtatccatg ggctgatttc ccgacgtctt atcaatatgt
     1681 cccaccttat aggctcattt ctgctcgttc tgcaacaccg ggatcacccg tttcatgtct
     1741 ttgtgccgcc ctcttccact cgggcaagga gcgaccgctc gtacgggaag ggctgaagga
     1801 cccggtcgag gacaccgtgg acgtacgaga tcgcgacccc gtagttcgtc accggcaccc
     1861 cggcatcctt gagcatccct atcctgttca gcatctccgc cctgttgatc atgcacccac
     1921 cgcagtggac ggcaagcttg tacgaggaga cgtcacggtc gagcacgggc cccgcgtgca
     1981 cgtcgaagga gaggtcccgg ccggtgtact gtctgagcca cctggggatc ttcaccctcc
     2041 ctatgtcgtc gggcaggggg tgatgggtgc aggcctccag gatgaggacc ctgtcgccgt
     2101 cagcgagacc ttcgatcgct ctgaccccct cgacgaaggt cgccaggtcg cccttgagcc
     2161 tcgagaagag gatggagaac gtcgtcagcc tcacgtccgg gggcaagtcg cccgagacct
     2221 tgagaaccac ctgggagtcg cagacggcga gcgagggcgg acgggcgagc cctctcatca
     2281 cgtactctat ctccctctcc ttggcgacga ccgccacggc gtcgttgtcg aggatgtccc
     2341 tgatgacctg gacctgcggc aggatgagcc tcccccttgg cgccccgagg tctatgggaa
     2401 cgatgaggag cacgcaatcg ccgtgaccga tgaggtcgcc cacgatcgtc gggggttcgg
     2461 gcctcagcga ggacgctgca tcgacgatcg cctgccttgc ccggtcgatg ccctcgccgg
     2521 tgagcgagct caccgtgacg tggcgcacgc ctgttcgatc gagcgtatcg acgacctctc
     2581 tcccgggacg ttcgacgtca gccttgttga acaccacgat cgtgactatc cctctcccgg
     2641 aaagccgtct catgaggtcc ctgtcgtcct cgtcgagccc ttgggccgtt gtcacgagga
     2701 gggcgacgtt caccttgccc aagacggcca gggtcttctt cacgcgcatc gtgccgagct
     2761 cgccggtgtc gtcg
//
LOCUS       DAOSCR010000372         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23583_length_2773_cov_3.463208, whole genome shotgun sequence.
ACCESSION   DAOSCR010000372 DAOSCR010000000
VERSION     DAOSCR010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23583_length_2773_cov_3.463208"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1113..2075
                     /locus_tag="PLT69_09090"
     CDS             1113..2075
                     /locus_tag="PLT69_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14631.1"
                     /translation="MNIRLVPAVVFAALVVSACSNDSAFEGISEKSGRDARVVDAQAS
                     LDGRNYDAAIDDLELIYTTTALDAEVSRMLASAYMGRAGIDFTLLMKQLPAQSSDHPF
                     DVMAEIISSPYETIDDGELYLLGNAVDGKTEKGEDTFEADLLEDLSTAKHILTVLDES
                     GLATADDLVQLGFASAAHFILHIGNQTAIALNPTMRFGSPDTWQYGIVPVPINTKAYR
                     YYKTSMTTDWKYNWAFVSPSHFKDDTAAGSRSSFQEDLENVRLAVRAVSQAYPGASDL
                     RNTMEAFLRSALGVEPGEDITEDLILEYSSSGVYSFANRLGEGR"
     gene            2103..>2773
                     /locus_tag="PLT69_09095"
     CDS             2103..>2773
                     /locus_tag="PLT69_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14632.1"
                     /translation="MRRMVFAAIAVLTVVSFETRAGELPRLYSDARVMAMGGAFVALS
                     DDACALFYNPAGLADVKSSTVNLPLPQAEFNNKAFDMFTDFNDTDFDSTSETGSFLRK
                     NLGDTGHAAVSFFPSYLRPRFAFGILGSARADLTSKNPVYPVLEVETVQDAAVCAGYA
                     LPAVGEDLLLGVGVKYLFRRSLDREYTVSDITSGDFENRLEDEMEEGSGALVDLDGSR
                     YDPRTG"
BASE COUNT          538 a          915 c          832 g          488 t
ORIGIN      
        1 tgagaacacc aaacaccgtg ggaaggcatg caccaaggct ttcgacatgc cctttcgagc
       61 gccaccccct gcccccggtc cacggggaac cagagaactc gtcgaatgcg tactgcccga
      121 tcttcaccca ccccccggtc taccgggaac caaaagtgta atacttttca catatgaaat
      181 aatttaccat tacttcggtg tcggccatcc cgccccctgg ggtatcgaga gggcacctgc
      241 cctgctcgag ctcaaagggt tccatctcat gggggaggcc ccttgccccg gcaaggggag
      301 agcgtcacca gttggcatcc tccatggtgg aatgccgctc cgttctttcc ccacgagggc
      361 aaatgaggga aaccgggtgc acaccggctc ttgttcatcg gtatgggttg tttcccgata
      421 gaaacaccct ttgccccggc acgaaagaca gtctcaaacc cgtgtcccac acggcgcaag
      481 gacgcgctgt tcctcacccg gcaccaagcc gggggatccg ggcgtccgcc tggcctgttc
      541 caacgggcag ggcctcaccc agtgggaaag gagtcttgtc cctgcacgag ggggacacct
      601 cccgcctggg caagggagac gacgcccatg caccgttttg tccccgcacg aggggtacac
      661 ctggaagaat gagtggacgc cgccagtctc caggggttct gtccaggcac gaggggcgcc
      721 cccgcatcgg cccctttcct gatggcggca ggggaaggtt ctgtccaggc acgaggggcg
      781 ccctctccac atcccctggt tcaccctttc cccctgctgg aagacgccgc ctcccccctt
      841 cccggtcctt atgcctgcga gaaaggaggc ggcagcgagt tcaaacgggg aattgacacg
      901 gtacgaggca gggcattatg gtagagacca tcgatcggcc gaccgtgctg ccgaccgtaa
      961 tctcggctga cagtcgccgt tcggactggt gtgattattg caacattgcg gttttgcact
     1021 ggaagacatc cgtgccgccc gagaaaggaa ccgcatggtg ccggtcacct gcatcgaacc
     1081 ccaagcgttc ggtgccgttg aggggaggga tcatgaacat acgtcttgtt cctgccgtgg
     1141 tctttgcggc cctcgtcgtg agtgcgtgca gcaacgacag cgcctttgag ggcatttccg
     1201 agaaatcggg ccgtgatgca cgagtggtcg acgcccaggc ctcgctcgac ggccgcaact
     1261 acgatgcggc catcgacgac ctggaactca tctacaccac gacggcactg gatgcggagg
     1321 tctcccgcat gctcgcctcc gcatacatgg gaagggccgg gatcgacttc accctcctca
     1381 tgaagcagct gccggcgcag tcctccgacc atcccttcga cgtcatggcg gagatcatat
     1441 cctcgccgta cgagacgatc gacgacggcg agctttacct cctcgggaac gccgtggacg
     1501 ggaagaccga gaagggtgag gacaccttcg aggcggacct cctcgaggac ctgtcgaccg
     1561 ccaagcacat ccttaccgtg ctcgacgagt cgggcctcgc cactgcggac gacctcgtgc
     1621 agctcggctt cgcatcagcg gcccacttca tcctgcatat cggcaaccag accgccatcg
     1681 ccctcaaccc gaccatgcgg ttcggctcac cggacacctg gcaatacggc atcgtgccgg
     1741 tccccatcaa cacgaaggca taccgctact acaagacctc catgacgacc gactggaagt
     1801 acaactgggc cttcgtgagc ccttcccact tcaaggacga cacggccgca ggctcgcggt
     1861 cctccttcca ggaagacctc gagaacgtgc gccttgcggt cagggccgtc tcccaggcct
     1921 acccaggcgc gtcggacctg aggaacacca tggaggcctt cctgagatcg gccctgggtg
     1981 tggaaccagg ggaagacatc accgaagacc tgatccttga gtacagctcg agcggagtgt
     2041 actcgttcgc gaaccggctc ggcgaggggc ggtgacagat gagaaggacg ggggatgaga
     2101 cgatgaggag aatggtcttt gccgcgatcg ccgtactgac ggtcgtttcg ttcgagacac
     2161 gggcaggcga gctgccccgc ctctacagcg acgcgagggt catggccatg ggcggggcct
     2221 tcgtcgcgct gtcggacgac gcctgcgccc tcttctacaa cccggccggt ctcgccgacg
     2281 tgaagtcatc aacagtgaac ctccccctgc cccaggccga gttcaacaac aaggcgttcg
     2341 acatgttcac agacttcaac gacaccgact tcgacagcac ctcggagacc ggttccttcc
     2401 tccgcaagaa cctcggggac acgggccacg cggcggtgtc gttcttcccc tcctacctga
     2461 ggccgcgttt cgccttcggc atccttggct ccgcgagggc ggacctgaca tcgaagaacc
     2521 ccgtctaccc cgtgctcgag gtcgagaccg tccaggacgc ggcggtatgc gcagggtatg
     2581 cccttccagc cgtgggcgag gatctcctcc tgggcgtggg ggtgaagtac ctctttcgga
     2641 ggagcctcga ccgggaatac accgtgtcgg acatcacctc cggcgacttc gagaacaggc
     2701 tcgaggatga gatggaagag ggctcgggcg cgctcgtgga cctggacgga agtcgctacg
     2761 acccgcgcac ggg
//
LOCUS       DAOSCR010000373         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23605_length_2772_cov_3.182554, whole genome shotgun sequence.
ACCESSION   DAOSCR010000373 DAOSCR010000000
VERSION     DAOSCR010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23605_length_2772_cov_3.182554"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLT69_09100"
     CDS             <1..311
                     /locus_tag="PLT69_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ14633.1"
                     /translation="AGYLALVPEGTTWKNEVPARFALLVSAYRPVAYARKVACPAFVV
                     YARRDSLIPHRSVRHMVSRMRDVQACVLDAGHFDVYTGALFEEVSARQAEFLARHLKP
                     "
     assembly_gap    461..470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <471..1167
                     /locus_tag="PLT69_09105"
     CDS             <471..1167
                     /locus_tag="PLT69_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ14634.1"
                     /translation="RLDEVAKNISGLGARVMTKKTDVSKREEVEDLARVVLAERKRVD
                     ILFNNAGVGLGGSFAETSIEDFEWLFSINFWGVLYGIKAFLPHMMEKRYGHIVNTSSL
                     AGLTALPGMSAYSAAKFAVAGLGEAIRSELRKYNIGVSTICPGVINTRVVEDGRMHLR
                     ESARANRDSVVEFYRRWGWPPERVAKAVLSAVRMNRSVVPVGPEAWVQWIGKRLSQTG
                     FEALSRIAGSFLM"
     gene            1283..2551
                     /locus_tag="PLT69_09110"
     CDS             1283..2551
                     /locus_tag="PLT69_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ14635.1"
                     /translation="MTYWFQDPAIKAILPLATSTAIKGLVTFFNSPALVIGANSLAEG
                     IMPQPSIVDIFNERCTTKRGFFVTDAFSERFAKKAVRTLEGAGFTTLVWNKALPEVPV
                     ETVHECAQEMTRFEPDMIFAVGGGSVIDTAKAAWILYERPDITDLGQVNPLGLLGLRK
                     KAGLAAIPTTSGTGSECTMVAVLHDTKTGRKVPIANGDLYPDIAILDPEFTMSMPPAL
                     TAGTGLDALAHAVDGIMTPASNDITDALGLRAIRLIFAYLPRAYTNGRDREARLRMLI
                     ASSMAGMCFGQNSCALTHGFGHAVGGTFGVHHGVSVGIFIPYAMQFYRPVTDKHLEIC
                     EALGIRGRDPDRLFSRLVSAVRRLLRATGVPTDLKGLAIEKAAFEAKMDELVEVALED
                     IDTFFSPRPITRDQARKLLVCAYEGRDVDF"
     gene            2563..>2772
                     /locus_tag="PLT69_09115"
     CDS             2563..>2772
                     /locus_tag="PLT69_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ14636.1"
                     /translation="MYGYHGRFLRVDLDRLTCEDMPIPEEVYRKYIGGATLGAHLIAP
                     HVRRGMDPLAGDAPLVFAVGPLTLSP"
BASE COUNT          498 a          859 c          966 g          439 t
ORIGIN      
        1 tggccgggta cctcgccctc gtccccgagg ggacgacgtg gaagaacgag gtcccggcga
       61 ggttcgccct cttggtttcg gcctacaggc ccgttgcata cgcacggaag gtcgcatgcc
      121 ccgcctttgt cgtgtacgcg aggcgggact cgctcatccc tcaccgctcg gtcaggcaca
      181 tggtctcgcg catgagggac gtccaggcgt gcgtgctcga cgccggccat ttcgacgtgt
      241 acaccggcgc actcttcgag gaggtatcgg cgaggcaggc ggaattcctc gcaaggcatt
      301 tgaaaccgta ggtgaggagg aggatatgcc aggcataaag gacttcaggg gaaaggtcgt
      361 ggtcgtcacg ggggcgggct ccggcatcgg gagggccacc gcgatcgcgt tcgcgcgcga
      421 gggggcggac ctcgtgatcg cggacgtccg cgaggagagg nnnnnnnnnn gaggctcgat
      481 gaggtggcga agaacatctc ggggcttggg gccagggtga tgacgaagaa gaccgacgtg
      541 tccaagaggg aggaggtgga ggacctcgca cgggtcgtcc tggccgagcg gaagcgtgtg
      601 gacatactgt tcaacaacgc gggcgtaggg ctcggcggga gtttcgcgga gacgagcatc
      661 gaggacttcg agtggctctt ctccatcaac ttctggggcg tgctctacgg gatcaaggcc
      721 ttcctgcccc acatgatgga gaagaggtac ggtcacatcg tgaacacctc gagcctggcg
      781 ggactgacgg ccctgccggg gatgagcgcc tacagcgcgg ccaagttcgc cgttgcgggc
      841 ctcggagagg ccataaggag cgagctcagg aagtacaaca taggggtgtc caccatctgc
      901 cccggggtca taaacacccg cgtggtggag gacggccgca tgcacctcag ggaatccgcc
      961 cgcgccaaca gggattccgt ggtggagttc taccgccggt ggggatggcc gcccgagcgg
     1021 gtggcgaagg cggtgctgag cgcggtgcgg atgaaccgct cggtggtccc cgtcggccct
     1081 gaggcgtggg tccagtggat cggcaagcgc ctgagccaga cgggtttcga ggcgttgagc
     1141 aggatcgccg ggtcgttcct catgtgaggc cgcctgccgt cagggcaggt cgggcgcatc
     1201 gagggcaagg ggcgtgcccc gcccgcatgc aggcgggcct gcccggaaga caccgtcgtg
     1261 aaacgagaga gaggagggaa acatgaccta ctggttccag gatcccgcga tcaaggcgat
     1321 cctgccgctt gccacctcga cggccatcaa ggggctggtg acgttcttca actcgccggc
     1381 gctcgtcata ggggcgaact cccttgccga gggcatcatg ccccagccgt ccatcgtgga
     1441 catcttcaac gagcggtgca ccaccaagag ggggttcttc gtgaccgacg ccttcagcga
     1501 gcgcttcgcg aaaaaggccg tgaggaccct cgagggcgcc gggttcacga ccctcgtgtg
     1561 gaacaaggcg ctgcccgagg tccccgtcga gacggttcac gagtgcgctc aggagatgac
     1621 gaggttcgag cccgacatga tcttcgccgt gggggggggc agcgtcatcg acaccgcgaa
     1681 ggccgcctgg atactctacg agcggcctga cataaccgac ctcgggcagg tcaacccgct
     1741 ggggctcttg ggcctgcgca agaaggccgg gctcgcggcc atccccacga cgagcgggac
     1801 cggctcggaa tgcaccatgg tcgccgtcct ccacgacacg aagacgggac gcaaggtgcc
     1861 tatcgcgaac ggggatctct accccgacat cgccatcctg gaccccgagt tcaccatgag
     1921 catgccgccc gcgctcaccg cgggcacagg gctcgacgcc ctcgcccatg cggtggacgg
     1981 catcatgacg ccggcctcga acgacatcac cgacgccctg ggtctcaggg cgatccgcct
     2041 catcttcgcc taccttcccc gtgcctacac gaacggccgg gacagggagg cgcgcctgcg
     2101 catgctcatc gcctcgtcca tggccggcat gtgcttcggc cagaacagtt gcgcgctcac
     2161 ccacgggttc ggccacgccg tgggcgggac gttcggcgtc caccacgggg tctctgtggg
     2221 catattcatc ccctacgcca tgcagttcta caggccggtc accgacaagc acctggagat
     2281 ctgcgaggcg ctgggcattc gcggcaggga ccccgacagg ctcttctcga ggctcgtgtc
     2341 ggccgtgagg aggctgttga gggcgaccgg ggtccccacg gacctcaagg ggctcgccat
     2401 cgagaaggcc gccttcgagg cgaagatgga cgagctcgtg gaagtagcgc tcgaggacat
     2461 agacacgttc ttcagcccca ggccgataac gagggaccag gcgaggaagc tgctcgtttg
     2521 cgcctacgag ggcagggacg tagatttcta ggaggatacg ccatgtacgg ataccacgga
     2581 cgtttcctgc gggtggatct cgacaggctg acatgcgagg acatgcccat accggaagag
     2641 gtctaccgga agtacatcgg cggtgcgacg ctgggtgccc acctgatcgc gcctcacgtg
     2701 aggaggggga tggacccgct ggcgggcgac gcccccctcg tgttcgcggt aggccccctc
     2761 accctgagtc cc
//
LOCUS       DAOSCR010000374         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23729_length_2761_cov_3.708056, whole genome shotgun sequence.
ACCESSION   DAOSCR010000374 DAOSCR010000000
VERSION     DAOSCR010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23729_length_2761_cov_3.708056"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..2133)
                     /gene="fusA"
                     /locus_tag="PLT69_09120"
     CDS             complement(46..2133)
                     /gene="fusA"
                     /locus_tag="PLT69_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ14637.1"
                     /translation="MKRSVPIERLRNIGIMAHIDAGKTTTTERILYYTGRSYKIGEVH
                     DGEAVMDWMEQEKERGITITSAATTCTWKDHVINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFCAVGGVEPQSETVWRQAERYRVPRIAFVNKMDRVGADFHRVVAQIADKLGARPA
                     PLQIPLGQEEGFAGVIDLVEMKAVVFDEETKGSNYSRVEIPAEYRARAEEYRERLVET
                     LCDVDDSLMEAYLEGGEISEKQLKTAIKKGTILGAITPVLLGSAFKNKGIQPLLDAVV
                     EFLPSPIEVGAVRGKTPSGEDAARAPREDEPFSALAFKVMNDPYVGQLTFIRVYSGVL
                     ESGQSVYNANTGKKERVGRLVRMFADKREELKDVFAGEIAAVLGLKNTITGHSLCDAD
                     RPIVLESMDFPEPVISIAIEPKGKTDEERLGFALSRLAMEDPSFKVQTDRETGDTLIS
                     GMGELHLEIIVDRLKREFGVDANVGRPQVAYRETVTKPVEETCKYVKQTGGHGQYAHV
                     VLTVDPLEPGAGFEFVNKIVGGVIPKEYIPAVKKGVEEALQVGPLAGYPVQDVRVTLI
                     DGSFHEVDSSDLAFKIAGSMAVKNALRKAAPIMLEPIMDVEVVSPVDNVGDVISDLSS
                     RRGRVLGMDTRGDVRVVAAQVPLAEMFGYATALRSLSQGRATFTMQVAHYEPVPANIS
                     DNLKVSRGGASHG"
     gene            complement(2158..2631)
                     /gene="rpsG"
                     /locus_tag="PLT69_09125"
     CDS             complement(2158..2631)
                     /gene="rpsG"
                     /locus_tag="PLT69_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ14638.1"
                     /translation="MPRKKRGSLKREIAPDPKYGDVQVAKFINSLMWDGKKSVSERIF
                     YDTLEILAGKTKEDPLVVFRKAFENVKPALEVRSRRVGGATYQVPQEVRPDRQDMLAR
                     RWIISAARARAEHTMSERLAAELMDAYNNRGTAIKKREDTHKMAEANKAFAHYRW"
     gene            complement(2642..>2761)
                     /gene="rpsL"
                     /locus_tag="PLT69_09130"
     CDS             complement(2642..>2761)
                     /gene="rpsL"
                     /locus_tag="PLT69_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322220.1"
                     /note="interacts with and stabilizes bases of the 16S rRNA
                     that are involved in tRNA selection in the A site and with
                     the mRNA backbone; located at the interface of the 30S and
                     50S subunits, it traverses the body of the 30S subunit
                     contacting proteins on the other side; mutations in the
                     S12 gene confer streptomycin resistance; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ14639.1"
                     /translation="GRVKDLPGVRYHIIRGVMDSEGVAERRKGRSKYGTKRPK"
BASE COUNT          394 a          951 c          836 g          580 t
ORIGIN      
        1 atcgtcccca cgttcacgtg cggcttcgtc ctcttgaacg tctccttagc catgacttgc
       61 acctcctcgg gaaaccttga gattatcgga tatattggca ggcaccggtt cgtagtgggc
      121 gacctgcatg gtgaacgtcg ccctgccctg cgacagggaa cgcagcgccg tggcgtagcc
      181 gaacatctcc gcgagcggca cctgggcggc caccaccctc acgtcgcccc tggtgtccat
      241 gccgagcacc cttcccctcc tcgaggagag gtcgctgatc acgtcgccca cgttgtccac
      301 gggcgagacg acctccacgt ccatgatcgg ctcgagcatg atgggggcgg ccttcctgag
      361 ggcgttcttc acggccatgg accccgctat cttgaaggcg aggtccgagg agtccacctc
      421 gtggaaggat ccgtcgatca aggtgacccg tacgtcctgg accgggtacc cggccagggg
      481 gcccacctgg agggcctcct ccacgccctt cttcaccgcg ggtatgtatt ccttcgggat
      541 gaccccgccc acgatcttgt tcacgaactc gaaaccagcg ccgggctcca gggggtccac
      601 cgtgaggacc acgtgggcgt actggccgtg gccgcccgtc tgcttgacgt acttgcaggt
      661 ctcctcgacg ggcttcgtga ccgtctcccg gtaggcgacc tgcggccttc ccacgttcgc
      721 gtccacgccg aactcgcgct tgagacggtc cacgatgatc tccaggtgca gctcgcccat
      781 gcccgagatc agcgtgtcac ccgtctccct gtccgtctgg accttgaacg aagggtcttc
      841 catggccagc ctcgacaggg cgaagccgag cctttcctcg tccgtctttc ccttgggctc
      901 gatcgcgatc gatatgaccg gctccgggaa gtccatggac tcgagcacga tgggccggtc
      961 cgcgtcgcac aggctgtgcc cggtgatggt gttcttgagc ccgagcacgg cggcgatctc
     1021 gccggcgaag acgtccttga gctcctcgcg cttgtcggcg aacatgcgga cgagcctccc
     1081 gacgcgctcc ttcttcccgg tgttcgcgtt gtagacgctc tgtcccgact cgaggacgcc
     1141 cgagtacacc ctgatgaagg tcagctggcc cacgtaggga tcgttcatga ccttgaaggc
     1201 gagggcgctg aacggctcgt cctcgcgcgg ggcccgggcg gcatcctccc cggacggggt
     1261 cttccctctg accgcgccca cctcgatggg cgacggcagg aactccacca ccgcgtccaa
     1321 aagcggctgg atgcccttgt tcttgaaggc ggagcccagg agcacgggcg ttatggcgcc
     1381 gaggatcgtg cccttcttga tcgccgtctt cagctgcttc tcggagatct cccctccctc
     1441 gaggtaggcc tccatgagcg agtcgtcgac gtcgcacagg gtctccacca gccgctcccg
     1501 gtactcctcg gcccgcgccc tgtattccgc cgggatctcc acccgggaat agttcgaccc
     1561 cttggtctcc tcgtcgaaga ccacggcctt catctccacg aggtctatga cgcctgcgaa
     1621 gccctcctcc tgtcccaggg ggatctggag gggggcgggc cgcgcgccga gcttgtcggc
     1681 tatctgggcc acgacacggt ggaagtccgc tccgacgcgg tccatcttgt tgacgaaggc
     1741 gatcctgggg acccggtagc gctcggcctg cctccagacc gtctccgact ggggctcgac
     1801 cccgccgacc gcgcagaaaa cggccaccgc cccgtccagg accctgaggg agcgttccac
     1861 ctcgatggtg aagtcgacgt ggcccggggt gtcgatgatg ttgatgacgt ggtctttcca
     1921 ggtgcaggtc gtggcggcgg aggttatggt gatgccccgc tccttttcct gctccatcca
     1981 gtccatgacc gcctcgccgt catggacctc gccgatcttg tagctccgac ccgtatagta
     2041 gaggatgcgc tcggtggtcg tggtcttccc ggcatcgatg tgcgccatga tgccgatgtt
     2101 cctgagtctc tctatgggta cggagcgttt cactgttctc ttcccttgcc ggtgcgacta
     2161 ccatcggtag tgcgcgaatg ccttgttggc ctcggccatc ttgtgggtgt cttccctctt
     2221 cttgatggcc gtgccgcggt tgttgtaggc gtccatgagc tcagccgcca ggcgctcgct
     2281 catcgtgtgc tccgcccggg cgcgcgcggc gctgatgatc cacctgcggg ccagcatgtc
     2341 ctgcctgtcg ggcctcacct cctggggtac ctggtaggtg gccccgccga ccctcctgga
     2401 gcggacctcg agggcgggct tgacgttctc gaacgccttc ctgaagacca cgaggggatc
     2461 ctccttggtt ttgccggcga ggatctcgag cgtgtcgtag aagatgcgct cgctcaccga
     2521 cttcttcccg tcccacatga gtgagttgat gaacttcgcc acctggacgt cgccgtactt
     2581 ggggtccggg gcgatctctc tcttcaggct tcctctcttc ttcctcggca tggcacaccc
     2641 tctacttcgg tctcttggta ccgtacttgg accggccctt gcggcgttcc gccacaccct
     2701 ctgaatccat gacaccccgg atgatgtggt acctgactcc gggcaggtcc ttcacgcggc
     2761 c
//
LOCUS       DAOSCR010000375         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23787_length_2757_cov_3.410437, whole genome shotgun sequence.
ACCESSION   DAOSCR010000375 DAOSCR010000000
VERSION     DAOSCR010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23787_length_2757_cov_3.410437"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            822..1421
                     /locus_tag="PLT69_09135"
     CDS             822..1421
                     /locus_tag="PLT69_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ14640.1"
                     /translation="MKRLTKRELRVEAIYKAALKVFAAYGFRKATIEDIANELGVGKS
                     SLYFYVRDKRELYEKAVAYGLTRWQDKVRKAVADEPDPERKFVVMAKKAYEHLVEDQD
                     LRRILERDPSLFPLDESRDPYAHINRASMNMIREVLANAIDAGRFRSVDLDAVPQLLF
                     AVYVMLVQKAYMFGQGPLAGIMFEDGLDIVLDGLRKPGR"
     assembly_gap    2149..2158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2159..2432
                     /locus_tag="PLT69_09140"
     CDS             <2159..2432
                     /locus_tag="PLT69_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOJ14641.1"
                     /translation="PDGKERYLFIQAEDGRVTRLVVGQGEPPKAEFTITGDYEVFASI
                     SRAELGSQRALMTGKLKVRGNLTKALRLAALADRLNKVLSTIPAVY"
     gene            2452..>2757
                     /locus_tag="PLT69_09145"
     CDS             2452..>2757
                     /locus_tag="PLT69_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOJ14642.1"
                     /translation="MPRILDPADPYYIDNPGRVRAMQEVMHEQDIDVYLGSRIRTLSW
                     ITDAFCPWRSFIVVPAEGMPTLFTFIIDALRVSDETWLDEDHVRAYAPMGGMDQIGAL
                     "
BASE COUNT          563 a          867 c          850 g          467 t
ORIGIN      
        1 tcatcgaacg caaggccaag agggcctgag ggaaagggcc accacggcgc acggatgggc
       61 gtgtgcaggg gtgaccctct ctccccatcg tcggaccatc ctccatccat cttccatgct
      121 tcggacattt ttctattgac tccaaaagga accatcgact ctccctgccg ctggacaatc
      181 ctttttccat cttcctctcc cacactcgat acccaggaca cctgcggact tcccgtcaag
      241 ggactcaaac agacgccgtt cgacccgaca cccggcagga accctcggta tcaccttctt
      301 tatcaagggt tcaccccgag accagtctcc ttcaggccgc atccggggtc caggcatccc
      361 cgactgttca tggacatgca ccgttacgct ccacctgcgc agcatgcagg ctagagccct
      421 gtgcctatcc tcaggggacg agctgcacgc gctgaggact gccctgtgtg ctcggaaaac
      481 gggctcgtcc gttccggccc ctgtcgcccc cctcgatgga gaagcgccgt gaggcggcgc
      541 atgtccatac gatgtgttcg gatcgacacc ccttcttcgt gtccgactga agagaccccc
      601 ccccggcagg ctgagccctc gtaaacaccc cggtgtccgg ctctccatac ggatcacggg
      661 gtgcacgtgt gacaggaagc acggcgccga gcatggccta ggcagacgag atcctcccct
      721 tgaagcatgt gaccaggcag gtatacgaca gggcttgaca cgctcaggcg tctcctgtaa
      781 cttgaacaaa tgatcatatt gttctataat tcgagaatca catgaaacgg ttgacaaagc
      841 gggaactcag ggtcgaggcg atctacaagg cggcgctcaa ggtgttcgcc gcctacgggt
      901 tcaggaaggc caccatagag gacatcgcca acgagctcgg cgtggggaag agctcgttat
      961 acttctacgt gcgcgacaag cgcgagctct acgagaaggc ggtcgcctac gggctcacga
     1021 ggtggcagga caaggtccgc aaggcggtcg cggacgagcc cgaccccgag cgcaagttcg
     1081 tggtcatggc gaaaaaggcc tacgaacacc tcgtcgagga ccaggacctc cgccgcatcc
     1141 tcgagcgtga cccgtcgttg ttcccgctcg acgagtcccg tgacccgtat gcccacatca
     1201 accgcgcatc aatgaacatg atccgcgagg tccttgcaaa cgccatcgac gcgggcaggt
     1261 tcaggagcgt cgatttggat gccgttcccc agctcctctt cgctgtctac gtcatgctcg
     1321 tgcagaaggc ctacatgttc ggccaggggc ctcttgccgg catcatgttc gaggacgggc
     1381 tcgacatcgt gctggacggc ctgaggaagc ccggccgatg atccctcttc gtcatggata
     1441 ccagcgaggt cagggtcgca ccttgcggaa aggcgggcag gcatcccatg cagaaggcag
     1501 ccgtgcccgg ctgcctcatg aagtaccggg cgcccggatg gagctgcggg caacgtgcgg
     1561 atggatgaag gagggtgcag cgcccgctgt gaacctcacc gcagggggca ccaggagctg
     1621 ccttcccgtg tgcctcgccg cgcgcgcagg aaaaggggga agacgtaagg gttttgcttg
     1681 aggcaccagg ggtttccttt cccttgtgtc ccatcgcacc ctccaacgag aaagaggggg
     1741 tcgacggaac aggccgaacg ggatactggg gggttcctgg ggcagagatc tcgttctcga
     1801 ggcccgagga catgaacgga gctgactgca caggatacag cggtcactgc ccgcccagcg
     1861 gcggatacgg gaaggtatcg gcgcaacagg gtatggacgt gggatggagg cgacacccga
     1921 gacccggggc gtgcgcctgc aggcaagaga cctcgtcggg tcgagcgtcg ccctcgggta
     1981 tccggcgcat ggcaacacga cggctcccgg ccggccaggc cgagatccgc tttccttaca
     2041 ccaggtgaag ggaggttcac atggaaaaga aggtcgtcta cctgagcgag gagtgggccg
     2101 cagaggccct gaggcgcatc aacgaggagc tctcacccga gcgaatgann nnnnnnnncc
     2161 ccgacggcaa ggagcgctat ctcttcatac aggccgaaga cgggagggtg acacgcctcg
     2221 tggtgggcca gggcgaacca cccaaggcgg aattcaccat cacgggagac tacgaggtct
     2281 tcgcgagcat ctccagggcc gagctcggct cgcagcgcgc gctcatgaca ggcaagctca
     2341 aggtgcgcgg caacctgacc aaggcgctca ggctcgcggc gcttgcagac aggctcaaca
     2401 aggtgctctc gacgatccct gccgtctact gacaaggaaa ggaggcttgc cgtgccgaga
     2461 atcctcgatc ccgccgatcc ttactacatt gacaaccccg gccgcgtcag ggccatgcag
     2521 gaggtcatgc acgagcagga catcgacgtg tacctcggta gccgcataag gacgctgagc
     2581 tggatcacgg acgccttctg cccgtggcgg agcttcatcg tcgtgcctgc cgagggcatg
     2641 ccgacactct tcaccttcat catagacgcc ctgcgcgtct cggacgagac ctggctcgac
     2701 gaggaccacg tccgggccta tgcacccatg ggagggatgg accagatagg cgcgttg
//
LOCUS       DAOSCR010000376         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23820_length_2754_cov_3.400148, whole genome shotgun sequence.
ACCESSION   DAOSCR010000376 DAOSCR010000000
VERSION     DAOSCR010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23820_length_2754_cov_3.400148"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLT69_09150"
     CDS             complement(<1..185)
                     /locus_tag="PLT69_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ14643.1"
                     /translation="MSDRNFTFSSESVTEGHPDKVADNISDAILDEIIANDKHAHVAC
                     ETLVTTGLVFVSGEITTT"
     gene            complement(355..1746)
                     /gene="thrC"
                     /locus_tag="PLT69_09155"
     CDS             complement(355..1746)
                     /gene="thrC"
                     /locus_tag="PLT69_09155"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ14644.1"
                     /translation="MSQGADPFMPDVAGEMGYVCIGCGGRYDIYEFIYTCPECGSLLK
                     IENAAFEDLKRRPASEWRNLFDSRRNSTTLEIQGVFRFHELILPIVPLKDVIYLGEGN
                     TPLVRANRELSQWVGAPFFVKNDGLNPSASFKDRGMASAISFLNHVIRVRGLTDVLGI
                     CASTGDTSAAAALYLSYLPKDKVRAVVLLPKGKVTPQQLSQPLGSGAKVIEMPGVFDD
                     CMRVVEELAQNYHVFLLNSKNPVRILGQKSYAYETAQQLGWDTKGLAVVVPIGNAGNI
                     TAVMDGFIDLLELGIIEELPLILGVQSEHADPIARWRSSGVFKPVKVRPSVAQAAMIG
                     DPVSFPKVKLNVERYFSDKFLVVSVSENEIMEGMLTANRHGHVVCTQGGESVAGLKKA
                     LETGLVPPGLTYVVDSTSHHLKFASFQERYFADDFDPAYGIQTREDLKNAPVTLDGKA
                     ATIASFLGLRRKT"
     gene            1914..>2754
                     /locus_tag="PLT69_09160"
     CDS             1914..>2754
                     /locus_tag="PLT69_09160"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOJ14645.1"
                     /translation="MEAMYTIDVMGAKKIASGKVREIFEVSDELLLIVTTDRLSAFDV
                     VLPDPIPAKGYVLNQTSLFWFEKCKALAANHIQSADVSEYPAPFKGDPRLEGRSVLVR
                     KAEPLPVECIVRGYLSGSGWKEYVRLGSVSGVRLPAGLRESDRLDEPIFTPSTKAAPG
                     THDENIDFDTAARLVGRSLLERVRELSLEIYRQACAHALERGIIIADTKFEFGLVGGD
                     LVWIDEALTPDSSRFWPLDGYQPGGPQPSFDKQYVRDYLETLDWDKTAPGPRLPEEVV
                     RKTS"
BASE COUNT          477 a          855 c          878 g          544 t
ORIGIN      
        1 gtggtcgtga tctcgcctga tacgaagacg agcccggtgg tcacgagggt ttcgcaggcc
       61 acatgcgcat gcttgtcgtt cgcgatgatt tcgtccagga ttgcatccga gatgttgtct
      121 gccaccttgt ccgggtggcc ttcggtgact gattcggacg agaacgtgaa attcctgtca
      181 gacatgaagc ttttcctcct tggcgtcgtg ctcacgcact ccttttgtat gcggtatgtg
      241 catgtgcagc agagtatgta cccgtggccc tgcctcgggc aagcgaaagc gtacgaaggt
      301 cgatgatccc gggtggcatg gtccgttcat ggtgccgggc gcgcttggca tcgctcacgt
      361 cttcctcctg agacccaaaa acgacgcgat ggtggccgcc ttgccgtcga gggtcaccgg
      421 cgcgttcttg aggtcttccc tcgtctgtat cccgtaggcg gggtcgaagt cgtccgcgaa
      481 gtaccgttcc tggaaggaag cgaacttgag gtggtgcgac gtggaatcca ccacgtacgt
      541 caggcccggg ggaacgaggc ccgtctcgag ggcctttttc agcccggcga cggattcccc
      601 cccctgggtg cacacgacgt ggccgtgcct gttggccgtc agcatccctt ccatgatctc
      661 gttctcgctc accgaaacca cgaggaactt gtcggagaag taccgctcca cgttgagttt
      721 caccttgggg aacgagaccg ggtcgcctat catggccgcc tgggccaccg acggcctcac
      781 cttgaccggc ttgaagacgc cgctcgatcg ccagcgggcg atcgggtccg cgtgctcgct
      841 ctggacaccc aggatcaggg ggagctcctc tatgatgccg agctcgagga ggtcgatgaa
      901 gccgtccatg accgccgtga tgttgccggc gtttcctatg gggaccacca cggcgagccc
      961 tttcgtgtcc cagccgagct gctgggcggt ctcgtacgcg tagctcttct ggccgaggat
     1021 gcgcaccggg ttcttcgagt tcagcaggaa gacatggtag ttctgcgcga gctcttcgac
     1081 cacgcgcatg cagtcgtcga agacgcccgg catctcgatg accttcgcgc ccgagccgag
     1141 gggctgggac agctgctggg gcgtcacctt gcccttgggg aggagcacca cggcccttac
     1201 cttgtccttc ggcaggtagc tgaggtacag ggccgccgca gccgaggtgt cgcccgtcga
     1261 tgcgcagatg cccagcacgt ccgtcagccc cctgacccgg atgacgtggt tcaggaacga
     1321 tatggcagag gccatgcccc ggtccttgaa gctggcggac gggttgagcc cgtcgttctt
     1381 gacgaagaac ggggcgccca cccattggct gagttcgcgg ttcgccctga ccaggggggt
     1441 gttgccctcg ccgaggtaaa tcacgtcttt cagcggcacg atggggagga tgagctcgtg
     1501 gaacctgaaa accccctgga tctcgagggt ggtgctgttc ctcctcgagt cgaagaggtt
     1561 cctccattcg gatgcaggcc tgcgtttcag gtcctcgaag gcggcgttct cgatcttgag
     1621 gaggcttccg cattccgggc aggtgtagat gaactcgtag atgtcgtagc gccccccgca
     1681 cccgatgcac acatagccca tctctcccgc gacatcgggc atgaagggat ccgcaccctg
     1741 cgacatggtt cgcctcgttt cctcccggaa tcgtccgtat actggcggcg gcccagattt
     1801 acgttcacgt ggagcccgcc ctaaggatca cactatgtat tacacgcgct tgacattttc
     1861 aactgttttt tgggatgctt gctccttatt tcacgacagg gaggcacgag gacatggaag
     1921 ccatgtacac catcgacgtc atgggcgcga agaagatcgc gagcggcaag gtcagggaaa
     1981 tcttcgaggt ctccgacgag ctgctgctca tcgtgaccac ggacaggctc tccgccttcg
     2041 acgtggtgct gcccgacccc atacccgcca agggatacgt cctcaaccag acctccctct
     2101 tctggttcga gaagtgcaaa gcgctcgccg ccaaccacat acagagcgcg gacgtctcgg
     2161 agtatccggc ccccttcaag ggcgaccccc gcctcgaggg gaggtccgtg ctcgtaagga
     2221 aggcggagcc tcttcctgtc gagtgcatcg tgagggggta cctctcgggg tcgggatgga
     2281 aggagtacgt acggctgggt tcggtctcgg gcgtgaggct gcccgcaggc ctcagggaat
     2341 cggatcgcct cgacgagccc atcttcaccc cgtccaccaa ggcggccccc ggcacccacg
     2401 acgagaacat cgacttcgac acggcggcga ggctcgtggg gcggagcctc ctcgagcggg
     2461 tccgggagct ctcgctggag atctacaggc aggcgtgcgc ccacgccctg gagaggggga
     2521 tcatcatcgc cgacaccaag ttcgagttcg gcctcgtggg gggagatctc gtgtggatcg
     2581 acgaggcctt gacccccgac tcctcgaggt tctggcccct ggacggttac cagccgggcg
     2641 ggccccagcc gagtttcgac aagcagtacg tgagggacta cctcgagacg ctcgactggg
     2701 acaagacggc ccctggcccg aggttgcccg aagaggtggt caggaagacc tccg
//
LOCUS       DAOSCR010000377         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23853_length_2751_cov_3.774852, whole genome shotgun sequence.
ACCESSION   DAOSCR010000377 DAOSCR010000000
VERSION     DAOSCR010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23853_length_2751_cov_3.774852"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLT69_09165"
     CDS             complement(<1..1008)
                     /locus_tag="PLT69_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HOJ14646.1"
                     /translation="MALKDVLHDIEGYDELEREIEILRKYGPDHVERAEEAARIMAVL
                     HPPRLSLKVSEIIEETPSTKTLRLVSAKGDLLPPFMAGQYVNLFVERDGVRTSRPYSI
                     SSSPTQTAYYDITVRAVEDGFVSRLLLEDTRPGDVFESTSPAGTFTHNPLFHGDDLVF
                     LAGGSGITPFMSMIRETADRGLDRKILLVYGSRDPGDIVFKDELERIAARRKNIAVHH
                     VISEPPEGYTGLAGFITADLVKRLAGDVTGKTFYVCGPAAMYERCLAELASLGLPRKR
                     VRTEAYGPPKNVTAQAGWPKHVSADDVFTVKISGGRVLSAKAGEPLMVSLEKAHIQIP
                     AQ"
     gene            complement(1019..2512)
                     /locus_tag="PLT69_09170"
     CDS             complement(1019..2512)
                     /locus_tag="PLT69_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ14647.1"
                     /translation="MAVYDVVVIGAGNGGLTAALTLARAGRKVALLERHNVPGGCATS
                     FVRGRFEFEVALHQLSGMGTERMPGPLRGILSEFGVLDKLEFVEMENLYRVVFPGGID
                     VTLPTDRQGIVEVLGGLFPKEKDAVASFLDLVYTYSLEMVQGVFFRDPLISKEKYPTF
                     FRYTFRNVEDVLDEFFTDPYLKAILSVYWGYTGVPPRLMPFSDLAVILWAYIEFKPFH
                     LKGGSQALSNALLDAYLKAGGEARFNCAARSIEVEGGRVRAVVTEHGDRLETSYVISN
                     ASTVHTYLDLVGSHRLPQDDLLKIKGRTVGPSAFTVYMGFDCTPEELGVFETSNFIVA
                     TIDMDSASEKMRTLEPQDWAMFSCYDVSDPDFSPPGTCQGALVCLQYADPWYRVEPHA
                     YAETKYRYADAMISLAERVFPNLRAHIEECEAASPLTHMRYLGHPGGAIYGFDQYAKD
                     SAFFLTNQSSIEGLYHAGSWVTGGGFQPTLTSGRSTAHAVLKAIKGR"
BASE COUNT          465 a          902 c          873 g          511 t
ORIGIN      
        1 ctgggccggg atctggatgt gggccttctc gagcgacacc atgagcggct cgcccgcctt
       61 ggcgctcaac acccttcccc ccgatatctt cacggtgaag acgtcgtctg ccgacacgtg
      121 cttgggccag cccgcctggg ccgtgacgtt cttgggcggc ccgtaggcct ccgtcctgac
      181 cctctttcga ggcaggccga ggctcgccag ctcggcgagg cacctctcgt acatggcggc
      241 cgggccgcac acgtagaagg tcttgcccgt cacgtcacct gcgagacgct tgacgaggtc
      301 ggccgtgatg aagccggcaa ggccggtgta tccttcgggc ggctccgata tcacgtggtg
      361 caccgcgatg ttcttgcgcc gcgcggctat cctctcgagc tcgtccttga agacgatgtc
      421 cccggggtcc ctgctcccgt acacgaggag gatcttccgg tcgagcccgc ggtcagccgt
      481 ctcgcggatc atgctcatga aaggcgtgat gccgctgccc cctgcgagaa acacgaggtc
      541 gtccccgtgg aacagcgggt tgtgggtgaa ggtgcctgcg ggagaggtgc tctcgaacac
      601 atccccaggc ctcgtgtcct cgagcaggag cctcgacacg aacccgtctt ccaccgcacg
      661 gaccgtgatg tcgtagtacg ccgtctgggt gggggaggag gatatgctgt agggcctgct
      721 cgtccgcaca ccgtctctct ccacgaagag attgacgtac tgccccgcca tgaaaggcgg
      781 cagcaggtcc cccttggcgg acacgaggcg cagggtcttc gtgctcgggg tctcttcgat
      841 gatctcggac accttgaggc tcaacctggg cgggtggagc acggccatga tgcgtgccgc
      901 ctcctcggcg cgttcgacgt gatccgggcc gtactttctc aggatctcta tctcccgttc
      961 gagctcgtcg tatccctcta tgtcgtgcag aacgtccttc agtgccatgg catcctcctc
     1021 atcttccctt gatcgccttg agcacggcgt gagcggtcga cctccccgag gtgagcgtgg
     1081 gctggaaacc gccgcctgtg acccacgagc ccgcgtggta caagccctcg atcgaggact
     1141 ggttcgtgag gaagaacgcg gaatccttcg cgtactggtc gaacccgtag atggcgccgc
     1201 ctgggtggcc gaggtatctc atgtgcgtca gcgggctcgc cgcctcgcat tcctcgatgt
     1261 gagccctgag gttcgggaaa accctctcgg cgagcgagat catggcgtcg gcgtagcggt
     1321 acttggtctc ggcgtaggcg tgcggctcga ccctgtacca cgggtccgcg tactggaggc
     1381 acacgagcgc accctggcag gtgcccggcg gcgagaagtc cgggtcggac acgtcgtagc
     1441 acgagaacat ggcccagtcc tgaggctcga gggtcctcat cttctcggag gccgagtcca
     1501 tgtcgatggt ggccacgatg aagttcgagg tctcgaagac gccgagctcc tccggggtgc
     1561 agtcgaaccc catgtagacc gtgaaggccg acgggccgac ggtcctcccc ttgatcttga
     1621 ggagatcgtc ctggggaagc cggtggctcc ccacgaggtc gaggtaggtg tggacggtgc
     1681 tggcgttcga tatgacgtag gatgtctcga gacggtcgcc gtgctcggtg acgaccgccc
     1741 tgaccctgcc gccttccacc tcgatggagc gcgcggcgca attgaacctc gcctcgccgc
     1801 ccgctttcag gtaagcgtcc aagagcgcgt tcgagagcgc ctgggacccg cctttcaggt
     1861 ggaacggctt gaactctatg tacgcccaca ggatcaccgc gaggtcggaa aacggcatca
     1921 gcctcggggg cacccccgtg tacccccagt acacgctgag gatcgccttg aggtaggggt
     1981 cggtgaagaa ctcgtcgaga acgtcctcga cgttcctgaa ggtgtacctg aagaaggtgg
     2041 ggtacttctc cttcgagatg agcgggtcgc ggaagaacac cccctggacc atctccaggc
     2101 tgtaggtgta gacgagatcg aggaaagagg cgaccgcgtc tttctctttg gggaagagcc
     2161 ctcccagcac ctcgacgatg ccctggcggt cggtgggcag cgtcacgtcg atccccccgg
     2221 ggaacaccac ccggtagagg ttctccatct ccacgaactc gagcttgtcc agcaccccga
     2281 actcggagag gatgcccctg agcggcccgg gcatgcgctc ggtccccatg ccgctcaact
     2341 ggtggagggc gacctcgaac tcgaatctgc cccgcacgaa gctcgtggcg cagcctcccg
     2401 gaacgttgtg gcgctcgagc aacgccacct tcctccccgc ccttgcaagc gtgagcgccg
     2461 ccgtgagccc cccgttaccc gcaccgatga ccaccacgtc gtagacggcc atagacaccc
     2521 ccgcgatctg atatgtttga agtttaactt taacctttat aaccccgagc gccctgcctg
     2581 tcaataggac aaagcagggc ctgggcgatc gacgtgcgcg tacgcgaccc tttccctgca
     2641 gagggagtgc cgatcgacga ggaacccgag caccacaagg acatgccagt tccccacccg
     2701 ccgggggtca cccgtacggc ttcgagtctc acctgggtgg gtcgccggct c
//
LOCUS       DAOSCR010000378         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23892_length_2747_cov_3.555349, whole genome shotgun sequence.
ACCESSION   DAOSCR010000378 DAOSCR010000000
VERSION     DAOSCR010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23892_length_2747_cov_3.555349"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2482
                     /locus_tag="PLT69_09175"
     CDS             <1..2482
                     /locus_tag="PLT69_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ14648.1"
                     /translation="DTLAAWGFTGLWLIGIWERSPASRTIKRLTGNPEAESSAYSIFE
                     YKIASDLGGDEAFENLKARAFARGIRMATDMVPNHMGIYSRWVLEHPDWFIQLDYPPF
                     PSYRFTGVNLSQDERYEIQIEDGYWNRTDAAVVFRWHDRHTGRTRYIYHGNDGTSMPW
                     NDTAQLDFLKEEVRRSVIDQTIAIARRFPIIRFDAAMTLAKRHFQRLWYPVPGYGGDI
                     PSRSGHSVPPEEFERLFPVEFWRELVDRVAQEAPDTLLLAEAFWLMEGYFVRSLGMHR
                     VYNSAFMNMLKAEENSKYRDVMKNVLRFNPEILRRFVNFMNNPDEEPAAVCFGKGDKY
                     FGVATLMATMPGLPMFGHGQVEGFAEKYGMEYRRSYWDEPVDGDLLGRHEREIFPLLK
                     RRRLFSGVENFVLYDCRMHDGHVNEDVFAYSNRLGDERVLVIYNNRFSEASGTLSRSV
                     GMNVGDASRRSIVQKSLSEGLGLKADEDLFYIFRDEKSGLEYVRNGADLARNGLRIHL
                     GAYKCHVFTSFREVRDVEARWKRVEQRLAGAGHPDVWALYREISVEKVLVPFRTLIAP
                     EDLRALVKEGRALGTVPPSFSSSLRSFFETACAFAQIEGEAVPSADRACLLLDALFTV
                     DRLRHKRLALRDERVRSFFSMLEPTPREDLEGWRIPLLWSVMVPVAALSQAAPARDNP
                     SSYIDEWMLGVGAQRAMESLGAEASAARREAAVVTVLAKHHELMVPGRVSFMLREMLL
                     DADVRDLLGVNHFGGTWWFNREAMNEFLGWASLCTALVALAGGSFDDRKALEIVCEVC
                     EGFDELSFKVDESRYEIERLASLLEAAA"
     gene            2564..>2747
                     /locus_tag="PLT69_09180"
     CDS             2564..>2747
                     /locus_tag="PLT69_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ14649.1"
                     /translation="MGKLFGTDGIRGVANVEPMTAETALKVGKAIALMCSRQDHTPRI
                     LIGRDTRQSGTMFESAI"
BASE COUNT          496 a          831 c          932 g          488 t
ORIGIN      
        1 ggacaccctg gccgcgtggg ggttcacggg tctctggctc atcggcatct gggagaggag
       61 cccggcatcc cgcaccatca agcggctgac cggcaacccc gaggccgagt cttccgccta
      121 ttccatcttc gagtacaaga tagcttccga cctcggcggc gacgaggcgt tcgagaatct
      181 caaggccagg gccttcgcca ggggtatcag gatggccact gacatggtgc ccaatcacat
      241 gggcatctat tcgcgctggg tccttgagca cccggactgg ttcatccagc tcgattaccc
      301 gccgttccct tcctaccggt tcacgggcgt gaacctctcg caggacgagc gctacgagat
      361 ccagatcgag gacgggtact ggaacaggac ggacgcggcg gtcgtgttca ggtggcacga
      421 caggcacacc gggcgaacgc ggtacatcta ccacggcaac gacggcacga gcatgccgtg
      481 gaacgatacc gcccagctcg atttcctcaa ggaagaggtt cgccgatcgg tcatcgacca
      541 gaccatcgcc atagccaggc gtttccccat catcaggttc gatgcggcca tgacgctcgc
      601 caagaggcat ttccagaggc tgtggtatcc cgtgccgggc tacggggggg acatcccctc
      661 gcgatcgggc cacagcgtgc cccccgagga attcgaacgg ctcttccccg tggaattctg
      721 gcgggagctc gtggatcggg tggcgcagga ggccccggac acgctgctgc tcgccgaggc
      781 gttctggctc atggaaggct acttcgtgag gagcctcggc atgcaccgcg tctacaacag
      841 cgcgttcatg aacatgttga aggccgagga gaacagcaag tacagggatg tcatgaagaa
      901 cgtgctgcgc ttcaacccgg agatcctgag gcggttcgtc aacttcatga acaacccgga
      961 cgaggaacct gcggcggtct gcttcggaaa gggcgacaag tacttcggcg tggccaccct
     1021 catggccacc atgccgggcc tgcccatgtt cggccacgga caggtcgagg gcttcgccga
     1081 gaagtacggg atggagtacc ggcgctcgta ctgggacgag cccgtggacg gagacctcct
     1141 cgggaggcac gaacgggaga tctttcccct cctcaagagg cgcaggctgt tctccggcgt
     1201 ggagaacttc gtgctctacg actgtcggat gcacgacggc cacgtgaacg aggacgtgtt
     1261 cgcctattcc aacaggctcg gggacgaacg cgtgctggtg atctacaaca accggttcag
     1321 cgaggcctcg ggaaccctgt cgcgctccgt gggcatgaac gtcggcgatg cgtcgaggcg
     1381 ctccatcgtc cagaagagcc tctcggaggg cctgggtctc aaggccgacg aggacctgtt
     1441 ctacatcttc agggacgaga agtcggggct cgagtacgtg aggaacggtg cagacctcgc
     1501 ccgcaacggg ctcaggatac acctcggggc gtacaagtgc cacgtcttca cgtcgtttcg
     1561 cgaggtcaga gacgtcgagg cccgttggaa gcgcgtcgag cagcggctcg caggcgctgg
     1621 tcacccggac gtgtgggccc tgtacaggga gatctctgtg gagaaggtcc tcgtcccgtt
     1681 caggaccctg atcgcgcccg aggacttgag ggccctcgtc aaggagggca gggccctggg
     1741 tacggtgccg ccgtcgtttt cctcctcgct gcgatccttc ttcgagaccg cctgcgcgtt
     1801 cgcgcagatc gagggcgagg ccgtgccatc ggcggatcgc gcatgccttc tcctggacgc
     1861 cctcttcacc gtggacaggc tcaggcacaa gcgccttgcg cttcgagacg agcgggtccg
     1921 gtcctttttc tccatgctgg aacccacgcc cagggaggat ctcgagggct ggaggatccc
     1981 tctcctgtgg tcggtgatgg tgcccgtcgc ggccctgtca caggcggctc ccgctcgtga
     2041 caacccgtct tcgtacatcg acgagtggat gctcggcgtg ggcgcacaga gggccatgga
     2101 gtcgctcggt gccgaggcgt ctgcagcccg acgtgaagcg gccgtggtga ccgtcctggc
     2161 caaacaccac gagctcatgg tcccgggcag ggtctccttc atgctcaggg agatgctcct
     2221 cgatgccgac gtgagggacc tgttgggggt caaccacttc gggggcacgt ggtggttcaa
     2281 cagggaggcg atgaacgagt tcctcgggtg ggcatccctg tgtacggcgc tggtggcgct
     2341 cgctggcggg agcttcgacg acaggaaggc cctcgagatc gtctgcgagg tgtgcgaagg
     2401 gttcgacgag ctctctttca aggtggacga gtccaggtac gagatcgagc gcctggcgtc
     2461 actgctcgag gctgcggcat gaggcaaggg tctcgtgcgg gccgatcgga cgaaggtggg
     2521 caagggtccc cactggggat ggggttttcg tggaggatga gagatgggga agctcttcgg
     2581 cacggacggg atacggggcg tcgccaacgt ggagcccatg accgcggaga ccgccctcaa
     2641 ggtcggcaag gccatcgcgc tcatgtgttc caggcaggac catacccctc gcattctcat
     2701 cgggagggac acgaggcagt cagggacgat gttcgagagc gcgatcg
//
LOCUS       DAOSCR010000379         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23913_length_2745_cov_3.435316, whole genome shotgun sequence.
ACCESSION   DAOSCR010000379 DAOSCR010000000
VERSION     DAOSCR010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23913_length_2745_cov_3.435316"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLT69_09185"
     CDS             complement(<1..469)
                     /locus_tag="PLT69_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOJ14650.1"
                     /translation="MKRTTVFLILAAFFAASIVSMGGCTTDPYTGEQKLSKTAIGGLV
                     GAATGAAVGAAVSKDKRKGALIGAGVGGLTGAGVGYYMDRQEAKLRQKLQGTGVSVTR
                     QGDNLILNMPGNITFRTNSADIDAGFYDVLNSVVLVLKEFDKTLIDVYGHTDNV"
     gene            complement(757..1365)
                     /gene="tmk"
                     /locus_tag="PLT69_09190"
     CDS             complement(757..1365)
                     /gene="tmk"
                     /locus_tag="PLT69_09190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ14651.1"
                     /translation="MTFEGGEGTGKTTCASLLCTHLEETGRAWVLLREPGGSTLSEEI
                     RTLFLERDMGPMVELLLVLASRRQNIDQIVRPALDAGKIVVIDRFVDSTIVYQCLVGG
                     LPVEEARSVMDLTGTWVEPDLTFVLDADPALTLERAGAATRFERRDVAYHERVRRAFL
                     DLAVDRRHRVLDASRDLEEVAADMVALFEEAAAARVSGHRGV"
     gene            complement(1368..>2745)
                     /locus_tag="PLT69_09195"
     CDS             complement(1368..>2745)
                     /locus_tag="PLT69_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOJ14652.1"
                     /translation="KGNKVILVRVETSPEDIHGMNVAQGVLTARGGMTSHAAVVARGM
                     GKCCVSGVTEMNIDYAKQKFTLGGVTVKKGDFISLDGTRGEVILGECKTVLPSLSGAF
                     GTIMEWADEFRRLGVRTNADTPKDAKVAREFGAEGIGLCRTEHMFFEADRILAVREMI
                     LANDEKGRRKALDKIKPMQKGDFKEIFRVMNGLPVTIRLLDPPLHEFLPNTEAELKEV
                     AKALKTTVDEVRAKRDALHEANPMLGHRGCRLGITFPEIYEMQVRAIIEAACEVKKEK
                     VEVQPEIMIPLVGHVNELASLKKMTVEVADEIIKSSGVKLKYMVGTMIELPRAAVTAD
                     KIAEEAEFFSFGTNDLTQTVFGLSRDDAGKFLPEYVEKKILPKDPFMALDTEGVGELV
                     RIGIEKGRKTRKKLKVGICGEHGGEPSSIEFCHKVAMDYVSCSPYRVPIARLAAAHAA
                     LRDGTQKK"
BASE COUNT          420 a          947 c          839 g          539 t
ORIGIN      
        1 ccacgttgtc cgtgtgccca tagacgtcga tcagggtctt gtcgaactcc ttgagcacga
       61 gcaccaccga gttgagcacg tcgtagaaac cggcatcgat gtccgcgctg ttggttctga
      121 aggtgatgtt gccgggcatg ttcaggatga ggttgtcacc ctggcgcgtg acgctcacgc
      181 cggtgccctg gagcttctgc ctgagcttcg cctcctgccg gtccatgtag tagccgaccc
      241 cggcgccggt gagcccgccc acgcccgcgc ctatgagcgc gcccttgcgc ttgtccttgc
      301 tgaccgctgc gccgacggcc gcacccgtgg ccgcacctac gagcccccca atggccgttt
      361 tgctgagctt ctgctcgccg gtgtaaggat cggtggtgca gccccccatg gagacgatgg
      421 acgccgcgaa gaacgcggcg aggataagga aaaccgtggt ccgcttcatg tgagccccct
      481 tttcgtaccg tgcatggttc tatacgatcc cctgaacctt cgttgccttc ccggctttca
      541 tagtaacgga atgcgcagga tcatgcaagg cccgcacggg cgccggtttt catggtacac
      601 cagggcccga cccgcggggg ggtatctccg ggagcccggc cgcatccctc catgtcgcat
      661 ccgccgggcg ccacctgcag gatcacgccg gcagggggtg tcatactgtc gacgctcgtg
      721 ggaacacgcc cggcatgaga ccggcggttc acgtcctcac accccgcggt gtcctgagac
      781 ccgtgccgcg gccgcctcct cgaagagcgc gaccatgtcc gccgccacct cttccagatc
      841 cctcgatgcg tcgaggaccc ggtgcctcct gtccacggcc agatccagga aggcacgcct
      901 cacgcgctcg tggtacgcga catccctgcg ttcgaaacgg gtggccgccc ccgccctttc
      961 cagcgtaagc gcggggtccg cgtccaggac gaaggtgagg tccggctcca cccaggtacc
     1021 tgtgagatcc atgacagaac gcgcctcttc gacaggaagc ccccccacca ggcactggta
     1081 cacgatggtg gagtccacga agcggtcgat gaccacgatc ttccccgcgt cgagggcggg
     1141 ccgcacgatc tggtcgatgt tctggcgtct ggatgcgagc acgagcagga gctccaccat
     1201 ggggcccatg tcccgctcca ggaacagggt gcggatctcc tcggacagcg tcgatccgcc
     1261 gggttccctc aggaggaccc aggccctgcc cgtttcctcg agatgggtgc acaggaggga
     1321 ggcgcaggtg gtcttgccgg tgccctcgcc cccctcgaag gtcacgatca cttcttctgc
     1381 gtcccgtccc tgagggctgc gtgcgcggcc gccagcctgg cgatgggaac ccggtacggc
     1441 gagcagctca cgtagtccat ggcgaccttg tggcagaact ctatggacga cggttcgccc
     1501 ccgtgctcgc cacagatgcc caccttgagc ttcttgcggg ttttcctccc cttctcgatg
     1561 cctatccgca cgagttcgcc cacaccctcg gtgtcgaggg ccatgaaggg gtccttgggc
     1621 aggatcttct tctccacgta ttcgggcagg aacttgcccg cgtcgtcgcg cgagagcccg
     1681 aacacggtct gcgtgagatc gttcgtgccg aaggagaaga actccgcctc ctcggctatc
     1741 ttgtcggcgg tcacggcggc cctggggagc tcgatcatgg tacccaccat gtacttgagc
     1801 ttcacgcccg aggacttgat gatctcgtcc gccacctcga cggtcatctt cttcaaggag
     1861 gcgagctcgt tgacatggcc gacgagcggg atcatgatct ccggctggac ctccaccttc
     1921 tccttcttga cctcgcaggc ggcctcgatg atggccctca cctgcatctc gtagatctcg
     1981 gggaacgtga tgccgagcct gcaccccctg tgcccgagca tggggttcgc ctcgtggagc
     2041 gcgtcccgtt tcgccctcac ctcgtcgacg gtcgtcttga gggccttggc cacctccttg
     2101 agctcggcct cggtgttggg caggaactcg tgcaggggcg ggtcgaggag cctgatcgtc
     2161 acgggcaggc cgttcatcac cctgaagatc tccttgaagt cgcccttctg catgggcttg
     2221 atcttgtcga gggccttcct gcggcctttc tcgtcattgg cgaggatcat ctcgcgcacg
     2281 gccaggatgc ggtcggcctc gaagaacatg tgctcggtgc ggcacaggcc tatcccctcg
     2341 gccccgaact cgcgcgcgac cttggcgtcc ttcggggtgt cggcgttggt cctcacgccg
     2401 agcctgcgga actcgtcggc ccactccatg atcgtgccga acgcgccgga gagcgagggc
     2461 aggacggtct tgcactcgcc aaggatcacc tcgcccctgg tcccgtcgag cgagatgaaa
     2521 tcgcccttct tcaccgtcac gccgccgagg gtgaacttct gtttcgcgta atcgatgttc
     2581 atctctgtca caccggacac gcagcacttg cccatgcccc tggccacgac cgcggcgtgc
     2641 gacgtcatgc cgcccctcgc cgtgaggact ccctgggcca cgttcatgcc gtggatgtcc
     2701 tcgggggagg tctcgaccct gaccagaatg accttgttcc ccttg
//
LOCUS       DAOSCR010000380         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23915_length_2745_cov_3.037175, whole genome shotgun sequence.
ACCESSION   DAOSCR010000380 DAOSCR010000000
VERSION     DAOSCR010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23915_length_2745_cov_3.037175"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..841
                     /locus_tag="PLT69_09200"
     CDS             86..841
                     /locus_tag="PLT69_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOJ14653.1"
                     /translation="MKVVFHEDFYRHYTSDPAAEPGRLEAVMEAIAGRVELIEPEPAA
                     LEDIQAVHTEAHIQHIKRIGLHEIASLAAGGAIKAAVTGMEEPCFALIRPPGHHASAN
                     TAWGFCFYNNMAVAIERLRREGKIERAHILDFDLHFGDGTVNILGRKGYVTIHNPESH
                     ERASYLKEVEERLAVAEADVFAVSAGFDNHEQDWGGLLATDDYRVMGAMVREAALKRG
                     AGLFAILEGGYNHRVLGSNVLALIEGMELGGQG"
     gene            1289..>2745
                     /locus_tag="PLT69_09205"
     CDS             1289..>2745
                     /locus_tag="PLT69_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOJ14654.1"
                     /translation="MKVSTVAEMRAMDRQAQERYGIPETILMENAGSASFEAMRRAYG
                     VRGKNVLVVAGPGNNGGDGFVVARRVVSAGGEARVLVVGDPQRYQGSAKLNLDILRKT
                     GVAVSVCGSAATVLEEAGACGVIVDALFGTGLARPVEGLHREVIDAVNECGKPVVSLD
                     IPSGINGDTGQVMGRAVRASITVTFGLPKPGNLLMPGAAWCGRLVVSHISFPPELYDD
                     PRIPISINEPLLPPVRDPDGHKGNFGEALFIAGAKGYYGAPCFAALSFMKAGGGYARL
                     AAPSSVIPFIATVSPEIVFHPMAETDSSSISARNKTRLLELAERADFVVMGPGVSLDE
                     ETASLVRELAGRMRKPLLIDGDGITAVCGDTSCISGRTSPTVITPHLGEMSRLTGKPV
                     EQIERDRIAALREAAGSLKATIVLKGAHSLIGHPDGRVVVNLSGNPGMATAGSGDVLT
                     GTIAAMFGLGLLFDDAVHAGVFLHGAAGDLAASSKG"
BASE COUNT          483 a          881 c          974 g          407 t
ORIGIN      
        1 cttgtcgctt gtggaaattg agcgcagggc gcggtcggtc caggtctgga cgacatcaac
       61 ccgcgcacag ggtcgggggg atctcatgaa ggtggtgttc cacgaggatt tctaccggca
      121 ctacacatcc gaccctgcgg ccgagccggg gaggctcgag gcggtgatgg aggcgatcgc
      181 cggcagggtc gagctcatcg aaccggagcc tgcggccctg gaagacatcc aggccgtcca
      241 caccgaggcc cacatccagc acatcaagcg catagggctc cacgagatcg cctcgctggc
      301 ggcagggggt gcgatcaagg ccgcggtcac gggcatggag gaaccctgct tcgccctcat
      361 ccgtccgccc ggccaccatg catcggcgaa cacggcgtgg gggttctgct tctacaacaa
      421 catggctgtg gccatcgagc gcctcaggcg cgaggggaag atagaacggg cgcacatcct
      481 cgacttcgac ctccacttcg gggacggcac ggtgaacatc ctgggcagga agggctacgt
      541 gaccatccac aaccccgaat cccacgagag ggcgtcctat ctcaaggaag tggaggagcg
      601 ccttgcagtc gccgaggccg acgtgttcgc cgtctccgcg ggcttcgaca accacgagca
      661 ggactggggg ggcctcctcg ccacggacga ctaccgggtg atgggcgcca tggtgaggga
      721 ggcggcgctc aagaggggcg ccgggctctt cgccatcctg gaaggaggct acaaccaccg
      781 ggtgctcggc tcgaacgtgc tcgcgctcat cgagggcatg gagctcgggg gacagggctg
      841 acgcagggtt cgagcacgca gcgacggcca agggcgcctg gatccgtgcg agccggcccc
      901 gcgtcccgtc cccgagaggg ctcgggcctc gcggaacgcc cggggttgga tgcggtgcag
      961 gagatccagg ccttctcggg cggtggggcg cgccgttgtc ggccggcagc ctcgacaggc
     1021 cgcccacgca ccggccctcc acggcgatcc aggctctcga gcgtgccgtt gcatcgaggg
     1081 cgccggccgt gcacttcgtg tttgcattcg ggcgagtcct gccccataac tactctcacc
     1141 ggtacgaccg ggctcaagaa gagcccgcta aaggcatatc agccggaggg gccgacacgt
     1201 cgtaccgggg gtacgggcgg ggacgagaag aaagccgggc ggcccatacg cccgacgaaa
     1261 tccatggtgt tgtaagggga ggagttccat gaaggtgagc accgtcgccg agatgagggc
     1321 catggacagg caggcgcagg aacgctacgg cataccggag acgatcctca tggagaacgc
     1381 cgggagcgcg tccttcgagg ccatgagaag agcatacggc gttcggggga agaacgtgct
     1441 ggtggtggcc gggcccggca acaacggagg ggacggcttc gtcgtcgcgc ggagggtcgt
     1501 ctcggcgggc ggagaggccc gcgtgctggt cgtgggcgac ccccagaggt accagggctc
     1561 ggccaaactc aacctcgaca tactcagaaa gaccggcgtc gccgtgtccg tgtgcggcag
     1621 cgcggcaacc gtgctcgagg aagcgggggc ctgcggcgtt atcgtggatg ccctgttcgg
     1681 gacggggctg gcgaggcccg tggagggtct ccaccgggag gtgatcgatg cggtgaacga
     1741 gtgcgggaaa ccggtggtga gcctggacat cccctcgggt atcaacggcg acacgggcca
     1801 ggtcatggga agggccgtca gggcatccat caccgtgacc ttcggcctgc ccaagccagg
     1861 caacctcctc atgccgggcg cggcatggtg cgggcgcctg gtcgtctccc acatatcctt
     1921 tccgccggaa ctctacgacg acccgaggat ccccatttcc ataaacgagc cgttgctgcc
     1981 gcccgttcgc gaccccgacg ggcacaaggg caacttcggc gaggcgctct tcatcgccgg
     2041 cgccaagggg tactacggtg cgccgtgctt cgccgcgctg tctttcatga aggcaggggg
     2101 cggctacgca aggctcgccg caccctcgtc cgtgatcccc ttcatcgcga ccgtctcgcc
     2161 cgagatcgtg ttccacccca tggccgagac cgactcctcg agcatctcgg caaggaacaa
     2221 gacgaggctg ctggagctgg ccgagcgggc cgacttcgtg gtcatgggcc ccggcgtatc
     2281 cctcgacgag gagaccgcct cgctcgtgag agagctggcg ggccgcatga ggaagcccct
     2341 cctgatagac ggggacggca tcaccgccgt gtgcggcgac accagttgca tctcgggccg
     2401 cacctcgccg accgtcatca cccctcacct cggcgagatg tcgaggctca ccgggaagcc
     2461 ggtggaacag atagagaggg acaggattgc ggctctcaga gaggctgcgg ggtccctgaa
     2521 ggccacgatc gtgctcaagg gcgcccacag tctcatcggg cacccggacg gtcgggtcgt
     2581 ggtcaacctg agcggcaacc cgggcatggc gacggcgggc agcggggatg tcctcaccgg
     2641 gacgatcgcg gccatgttcg ggctcgggct tctcttcgac gacgcggttc acgcaggggt
     2701 cttcctccac ggcgcagccg gggacctcgc cgcatcgtcc aaggg
//
LOCUS       DAOSCR010000381         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_23923_length_2744_cov_3.181480, whole genome shotgun sequence.
ACCESSION   DAOSCR010000381 DAOSCR010000000
VERSION     DAOSCR010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23923_length_2744_cov_3.181480"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..891)
                     /locus_tag="PLT69_09210"
     CDS             complement(1..891)
                     /locus_tag="PLT69_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ14655.1"
                     /translation="MDFDIILQAIINGLLTGSYYGLAALGLTLIFGVIKVINFAHGSL
                     LMVGMFTAYWIVKLTGWNPYAALLVVVPLLFLFGYGLQGFLIKPVLIAQRDVREPTEV
                     IIVTTGVWYILDNLALMLFGAEYRVVQTSVSGQIFMLGDYIFPKPQLYGFIITAATSF
                     LLVWFLKYTKMGKAIRAASLDRDAASLMGINQYRVYNIAFGIGAAIAGIVGCVLIPFY
                     YVFPTVGVVFDVKCFIIVVLGGLGSIPGALVGGAIIGLIESIFAQFIASTWTELIVYV
                     VFLAVLFIRPAGLFGEKRDW"
     gene            complement(978..2243)
                     /locus_tag="PLT69_09215"
     CDS             complement(978..2243)
                     /locus_tag="PLT69_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ14656.1"
                     /translation="MKRVFRHTLKGVVVFGLSVLLVLSAGQASKAAGPKEIKIGNILP
                     LSGPSASVGLQNKQAVELATEKINAAGGIKSLGGAKLVNVFTDSKGDPNVGVTAAEQL
                     INTEKVVLISGAWNSAVTYPSTQVAERYQVPYVVPVSVADKITERGFKYTFRIAAKDS
                     WWTDYQFKFLEEQKKLTGTKIETMAFVYENGDWGTGMAKQWHERAKKFGYKVVLDEAY
                     PSTASDLTPVVMKIKTKKPDVILLTSNAADAILLTNTMATMKVKAKAIIGTGGGHADP
                     AFRKNTGKNCEYMFDVVEWETDLGRPQIPALNAEFKKRYGYDLAGESVDAFASVYVIA
                     DALERAKSTDPKKIRDALAATKLCVGQGKLGIDILSYDCIEFDKTGQNKHAGIVLVQF
                     RDVKGTMERVTVFPTKVARPGFKAVFPMP"
     gene            complement(2441..>2744)
                     /locus_tag="PLT69_09220"
     CDS             complement(2441..>2744)
                     /locus_tag="PLT69_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ14657.1"
                     /translation="KKVRKYFNELMKKHEVIGDVRGLGPMLALELVKDRKTKEPAPEE
                     TASVVKYAFEKGLNILSCGQYSNVIRTMMPLVITDEQLAKGVSILDEAFASLKKKK"
BASE COUNT          481 a          851 c          853 g          559 t
ORIGIN      
        1 tcaccaatcc cgcttctcgc cgaacaaccc ggcgggcctt atgaagagca cggccaggaa
       61 gaccacgtag acgatcagct cggtccaggt ggaggcgatg aactgggcga agatagactc
      121 gatgaggccg atgatcgcgc ccccgacaag ggctccgggt atgctcccca gaccccccag
      181 gaccacgatg atgaagcact tcacgtcgaa gaccacgcca accgtgggaa agacgtagta
      241 gaatggaatg aggacgcacc ccacgatgcc ggcgatcgcg gccccgatgc cgaaggcgat
      301 gttgtagacg cggtactggt tgatccccat gaggctcgcg gcgtctcggt cgagggaggc
      361 ggccctgatc gccttgccca tcttcgtgta cttgaggaac cacacgagga ggaacgaggt
      421 cgcggccgtt atgatgaagc cgtagagctg gggcttgggg aagatgtagt cgccgagcat
      481 gaatatctgc cccgagacgc tcgtctggac caccctgtac tcggccccga agagcatgag
      541 ggcgaggttg tcgaggatgt accacacgcc cgtggtcacg atgatgacct cggtgggctc
      601 gcgcacgtcc ctctgggcga tgaggacagg cttgatgagg aacccctgga gaccgtagcc
      661 gaagaggaac aagagcggca cgaccaccag cagggccgcg tacgggttcc accccgtgag
      721 cttgacgatc cagtaagccg tgaacatgcc caccatgagc agagatccgt gggcgaagtt
      781 gatgaccttg atgaccccga agatgagggt cagccccagg gcggcgaggc cgtagtagga
      841 tcccgtgagg agcccgttga tgattgcctg aaggatgatg tcgaagtcca tggtaacctc
      901 ggatctggtc ggattgggga tgccgggccg ggcccggcac ccctcatgtg tcctgttatc
      961 ccgctccttt cataccctca gggcatgggg aagacggcct tgaagcccgg gcgcgccacc
     1021 tttgtcggga acacggtgac cctctccatg gtgcccttga cgtccctgaa ctgcacgagc
     1081 acgatccctg cgtgcttgtt ctggcccgtt ttgtcgaact cgatgcagtc gtaggagagg
     1141 atgtcgatgc cgagcttgcc ctgaccgacg cagagcttcg tcgcggcgag cgcgtccctg
     1201 atcttcttgg ggtcggtgga cttggcgcgc tcgagcgcgt ctgcgatcac gtagaccgac
     1261 gcgaaggcgt ccacggactc accggccagg tcgtacccgt agcgcttctt gaactcggcg
     1321 ttcaaggccg ggatctgggg cctgccgaga tccgtctccc attccaccac gtcgaacatg
     1381 tactcgcagt tcttgccggt gttcttcctg aacgccgggt ccgcgtgccc gccgccggtc
     1441 ccgatgatgg cctttgcctt gaccttcatg gtcgccatgg tgttggtgag caggatggcg
     1501 tcagcggcgt tcgacgtgag caggatgacg tcgggcttct tggtcttgat cttcatgacg
     1561 accggggtca ggtctgacgc ggtgctcggg tacgcctcgt cgaggacgac cttgtagccg
     1621 aacttcttcg cccgctcgtg ccactgtttg gccatgccgg tgccccagtc cccgttctcg
     1681 tacacgaagg ccatggtctc gatcttcgtg ccggtgagct ttttctgttc ctcgaggaac
     1741 ttgaactggt agtcggtcca ccagctgtcc ttggcggcga tgcggaaggt gtacttgaac
     1801 ccgcgctcgg tgatcttgtc agccacggag acgggcacca cgtacgggac ctggtaacgc
     1861 tccgccacct gtgtggaagg gtaggtcacc gccgagttcc atgcgcccga gatgaggacc
     1921 accttttccg tgttgatgag ctgctccgcc gcggtgacgc cgacgttcgg gtcccccttg
     1981 ctgtcggtga acacgttcac cagcttggcg ccgcccagag acttgatgcc gcccgccgcg
     2041 ttgatcttct ccgtggcgag ctcgacggcc tgcttgttct ggaggcccac cgatgcggac
     2101 ggcccggaca gcggcaggat gttgccgatc ttgatctctt tcgggccggc ggccttcgag
     2161 gcctgtcccg cggacagcac caggagaacc gagagcccga agaccacgac acccttcagg
     2221 gtatgccgga aaaccctctt cataacgctt accccccttc attgatttgt gttggccccc
     2281 acgggggccg ctctgacacc gaagacatcc atgcgttcag ggttccggga cggggggagt
     2341 acccccgccc ccctgagccg ctccatgaca cacagggcaa ggtggagacg acatgcccga
     2401 tgcgttcttc ccttttcctt tcgcgacccc catgtgtccc ctatttcttc ttcttcaatg
     2461 acgcgaacgc ctcgtccagg atggagacac cctttgccag ctgctcgtcg gttatgacga
     2521 gcggcatcat ggtgcggatc acgttgctgt actggccgca ggacaggatg ttgagcccct
     2581 tttcgaaggc gtacttgacg acggatgccg tctcctcggg cgccggttcc ttggtcttcc
     2641 ggtccttgac gagctcgagg gcgagcatcg ggcccaatcc tctgacgtcg ccgatgacct
     2701 cgtgcttctt catgagctcg ttgaagtact tcctgacctt cttg
//
LOCUS       DAOSCR010000382         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24077_length_2731_cov_4.087070, whole genome shotgun sequence.
ACCESSION   DAOSCR010000382 DAOSCR010000000
VERSION     DAOSCR010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24077_length_2731_cov_4.087070"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..366
                     /locus_tag="PLT69_09225"
     CDS             73..366
                     /locus_tag="PLT69_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14658.1"
                     /translation="MERTRSSVVVNEKRIEILDTQAQGRSIKTRVIDEQGREVPPGAV
                     RAGTRVLAKGSTAFDERARTLVYLATEVTILGRPIDLRDEVQRKRYLEPARPW"
     gene            complement(422..1528)
                     /locus_tag="PLT69_09230"
     CDS             complement(422..1528)
                     /locus_tag="PLT69_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14659.1"
                     /translation="MTRACSLACAALILAASILANGPLLAETTVTVTGVAPAAGGDTS
                     PVEKRAFEDALAKAYLLSAIRHVPSSSIPALSAGIGAFVTQRAGKDVIRYKVISKSQS
                     QAAMMVTYDVTIDDEPLKAWLAAEKLSTPVFMRPKVLLAITTRGPRPQERHEWWTQPT
                     PASYSPFEEVMARRLRSSGENVIGPPAQKALSVKTEDWALVLGRQAGADIVVYGLLTH
                     TRPEGTTVNSRLDLFVADVRSNSRLASTSLTLRGSLDVEVMNELVVSAVWEQVRSAIV
                     ARTVSFSPASREKTLCIQGMRDNEQYQEILGAIRSLGIVSSVTVSGISGRKLCHTVRT
                     AADLQEVMRGLSQRLAIQADMEVDEDTALLRVLN"
     gene            complement(1525..1761)
                     /gene="hfq"
                     /locus_tag="PLT69_09235"
     CDS             complement(1525..1761)
                     /gene="hfq"
                     /locus_tag="PLT69_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOJ14660.1"
                     /translation="MGKVVFNVQDQFLNQARKERVPIVVTLIDNSKVDGFVKSFDPFC
                     ILIQTERPILIYKHAVVKIEPTEVNSKLKDFLAG"
     gene            1923..>2731
                     /locus_tag="PLT69_09240"
     CDS             1923..>2731
                     /locus_tag="PLT69_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOJ14661.1"
                     /translation="MHDHAVKSMANVPDSIRSRSEELRNLIEYHNHRYYVLDSPEISD
                     AAYDELMRELMRIEEQYPELATDDSPTRRVGAPPLEEFAPMVHRVPLLSMDNAMDPSE
                     VAAFHERTARSLGKDEVVYCCEPKFDGLAIELVYEGGVLTRAGTRGDGSVGEDVTRNV
                     RTIRSVPLRLRTATPPGLIEIRGEVVIFRKDFEELNRRRTASGEAPFANPRNAAAGSL
                     RQLDPAVTASRPLVFFAYGVSERAFLGLDSQYEVLMRLKEMGFGINDDTRR"
BASE COUNT          517 a          800 c          910 g          504 t
ORIGIN      
        1 gccccggccc gggcgggcgt ggtctagaac gcgctgagat cgtgggagcc gttcacgatc
       61 gagggcgtcg tcatggagag gacgaggtcg tccgtcgtgg tcaacgagaa gaggatagag
      121 atcctggaca cccaggcaca gggaaggtcc atcaagaccc gggtgatcga cgagcagggc
      181 cgcgaggtgc ccccgggcgc cgtgagggcg gggacgaggg tccttgcgaa aggctcgacg
      241 gccttcgacg aaagggcaag aaccctcgtc tacctcgcga ccgaggtcac catcctggga
      301 aggcccatag acctcaggga cgaggtgcag aggaagaggt acctcgagcc tgcaaggccg
      361 tggtgacagg gccgggagtg ccggcacgag aggggctccg cgcgggatgc ggcgcccctt
      421 ttcagttgag gaccctgagc agggccgtgt cctcgtccac ttccatgtca gcctggatcg
      481 cgagcctctg ggagaggccc ctcatgacct cttggaggtc ggctgcggtc cgcacggtgt
      541 ggcagagctt ccggcctgag atccccgaga cggtgacgct cgagacgata ccgagcgacc
      601 gtatggcccc caggatctcc tgatactgct cgttgtcgcg catgccctgg atgcaaaggg
      661 tcttctcgcg ggaggcgggg gagaaagata ccgtgcgcgc gacgatggcc gatctcacct
      721 gttcccatac ggcgctgacc acgagctcgt tcatgacctc cacgtcgagc gagcccctga
      781 gggtgaggga cgtggatgcc aggcgggagt tcgagcggac gtccgccacg aagaggtcca
      841 ggcgcgagtt gacggtggtc ccttccgggc gcgtgtgggt caagaggccg tagaccacga
      901 tgtccgcacc tgcctgtctc cccaggacca gcgcccagtc ctcggtcttg acggacaagg
      961 ccttttgggc cggcgggccg atcacgttct ccccggagga cctcaggcgc ctcgccatga
     1021 cctcctcgaa ggggctgtat gaggcggggg tgggctgggt ccaccactcg tgtcgttcct
     1081 gcggcctcgg gcccctggtg gtgatcgcga gcagcacctt gggcctcatg aagaccggcg
     1141 tggagagttt ctcggcggcg agccacgcct tgaggggctc gtcgtcgatg gtgacatcat
     1201 aggtgaccat catggcggct tgtgactgtg actttgatat gaccttgtat cggatcacgt
     1261 ctttcccggc acgctgggtg acgaacgccc cgataccagc cgacagggct gggatcgagg
     1321 atgacggtac atgcctgatg gcggacagga ggtatgcctt ggcaagggcg tcctcgaagg
     1381 cccgcttctc gacgggagag gtgtcgccgc ctgcagccgg cgccacgccg gtcacggtga
     1441 cggtcgtctc agcgagcagg ggtccgttcg caaggatcga cgctgcaagt atcagcgccg
     1501 cacaggcaag cgaacaagcg cgggtcatcc tgccaggaag tccttcagct tggagttgac
     1561 ctcggtcggc tcgatcttca ccactgcatg cttgtagatg aggatgggcc tctcggtctg
     1621 tatcaatatg cagaaggggt cgaaactctt gacgaaaccg tccaccttgg agttgtcgat
     1681 cagggtcacc acaatgggga ccctttcctt gcgtgcctgg ttcaggaact ggtcctgtac
     1741 attgaacaca actttaccca ttgcaacctc cttgcgacga gatctcatcg gcacaatacc
     1801 aacccctgtc tgatgtattt gttgtgaaca acgtttgcgt tgcacccatg aatattaatc
     1861 agaaagatct tttgatcaag tcttttttgt gttacagcac gatttctata gcgaaagcga
     1921 tcgtgcatga tcacgcggtg aaatccatgg ccaacgtccc ggattccata aggagtcgct
     1981 ctgaggagct caggaacctc atcgagtacc acaaccaccg gtactatgtg ctcgactcgc
     2041 cggagatatc ggacgccgcc tatgacgagc tcatgcgcga attgatgcgg atcgaagagc
     2101 aatacccgga gctcgccacc gatgactcgc cgaccaggcg cgtgggggcc ccgcccctcg
     2161 aggagttcgc tcccatggtg catcgcgtgc ctctgttgag catggataac gccatggacc
     2221 cttccgaggt cgccgctttt cacgagagga cggcgaggag tctggggaaa gacgaggtgg
     2281 tctactgctg cgagccgaag ttcgacggcc tggccataga gctcgtctac gagggggggg
     2341 tcctgacgcg tgccggtacc cgcggagacg gcagcgtcgg ggaggacgtc acccggaatg
     2401 tccgtaccat ccggagtgtg ccgctccgcc tgaggaccgc gaccccaccc gggctgatag
     2461 agatcagggg cgaggtggtg atcttcagga aggacttcga ggagctcaac aggaggagga
     2521 cggcaagcgg cgaggcgccg ttcgccaacc cccgcaacgc ggcggccggg tcactcaggc
     2581 agctcgaccc tgcggtgacc gcctcgaggc cgctggtctt cttcgcctat ggtgtctcgg
     2641 aaagggcgtt tctcggtctc gattcccagt acgaggtgct catgcgcctg aaggaaatgg
     2701 gcttcgggat caacgacgat acgaggaggt g
//
LOCUS       DAOSCR010000383         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24078_length_2731_cov_3.103139, whole genome shotgun sequence.
ACCESSION   DAOSCR010000383 DAOSCR010000000
VERSION     DAOSCR010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24078_length_2731_cov_3.103139"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLT69_09245"
     CDS             complement(<1..661)
                     /locus_tag="PLT69_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ14662.1"
                     /translation="MGEHTGAFQDMLEALERFASENIAPREDLGSSTEFPFDLWKRMG
                     EEGLLGASVPEEYGGSGLGYGAISRLAMALARRGGCLGIVLSWLMHQIACRFVMAGMA
                     SKDLMDTYLPGLAAGEATVSIAISEPKTGAHPRHMETRAEKTARGYLVSGEKAFLTNG
                     PIASLFVVVAVTKTEGGLKSFSSMVVRRETEGLEVLPPLDVGFLHPCPHGAITMDRCA
                     VG"
     gene            complement(672..2048)
                     /locus_tag="PLT69_09250"
     CDS             complement(672..2048)
                     /locus_tag="PLT69_09250"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ14663.1"
                     /translation="MMSPLLKFVACCRAAGYRISTSEVIDCASQLEHVDVLDEDEFRA
                     VLRANFAKSRNEQLRFDRLYRLFFSELKGDFSEEEGLSKGVSEIIDSLREAMNGDEAY
                     RAIVEFLSGDPYRFLDLIRQIHSESDSANLGSKFSLGPLSSKLRVLLNLNRVREEVAA
                     AIDGNRLRYDPGMRAELSRHLADRIDSAQALLLREPRSYADDALGYRTHEKRLMELGE
                     RSFSALSARELEEVRAALDRLVRKLKDIVSRRYAASSRGVLDVKKTLRRAERYHGVPL
                     EIVYRKRPPKKAKIVVLCDISSSVWAAARFMLNVLYSLHECFERVNSFVFVSGVREVT
                     EAFENNDPDVAINLILKDPEFNYYVPTDYGETFRQFKLRHMDMLTKRTTLIIMGDART
                     NYLHPEDGILEEMRERSRRVIWLNPEPRSSWNTGDSEMYAYGKFCNEVRQCRNLNELV
                     AFIEELVI"
     gene            complement(2045..>2731)
                     /locus_tag="PLT69_09255"
     CDS             complement(2045..>2731)
                     /locus_tag="PLT69_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ14664.1"
                     /translation="EWEYAKQLLYTQMVKEKIGDVIDGAGSLAEAVDRIAEQEDAFFS
                     ERFIQPRPLLRAITSNDPVVLLIDEVDKSDPEFEAFLLELLSDFQVSIPEIGTKRASV
                     IPFVVLTSNNYRDMSDALKRRCVHIYIDYPDRDLERRIVTVKIPGIPDRLADKVVDAV
                     RRIREVDLKKKPCISETIDWARSLLALSVEDITPEILVETVGVLCKYRSDADTVRARA
                     AELLGQGCTA"
BASE COUNT          450 a          922 c          818 g          541 t
ORIGIN      
        1 cccccaccgc gcaccggtcc atcgtgatcg ccccatgggg gcacgggtga aggaaaccga
       61 catcgagggg gggcagcacc tcgagcccct cggtctccct cctgaccacc atcgagctga
      121 agctcttgag cccgccttcg gtcttggtga cggcgacgac cacaaacagg gacgcaatgg
      181 gcccgttcgt gaggaaggcc ttctcgccgc tcacgaggta gcctcgagcc gtcttctcgg
      241 cacgcgtctc catgtgcctg gggtgggcgc ccgttttcgg ctcgctgatc gcgatcgaga
      301 ccgtcgcctc ccctgcggca agcccgggga ggtacgtatc catgaggtcc ttgctggcca
      361 tgccggccat gacgaagcgg caggcgatct ggtgcatgag ccagctcagg acgataccca
      421 ggcacccgcc ccttcgggca agcgccatgg cgagccggct tatggcgccg tacccgaggc
      481 ccgagccccc gtactcctcg ggcaccgatg cgccgaggag cccctcctca cccatccgct
      541 tccacaggtc gaaggggaac tccgtgctcg atcccaggtc ttcacgcggg gctatgttct
      601 ccgacgcgaa cctctcgagg gcttcgagca tgtcctgaaa cgcgcctgtg tgctctccca
      661 cggccctctt cctagatcac gagctcctca atgaaggcca cgagctcgtt gaggttcctg
      721 cactgcctca cctcgttaca gaatttgccg tatgcgtaca tctcgctgtc accggtgttc
      781 cagctcgaac gcggctcggg gttcagccat atgaccctcc tcgacctctc cctcatctcc
      841 tcgaggatcc cgtcctcggg gtggaggtaa ttggtccgtg cgtcgcccat gatgatgagc
      901 gtggtgcgct tcgtgagcat gtccatgtgc ctgagcttga actggcggaa ggtctccccg
      961 tagtcggtgg ggacatagta gttgaactcc gggtccttga ggatgaggtt gatggcgaca
     1021 tccgggtcgt tgttctcgaa cgcttccgtg acctcgcgca cccccgatac gaacacgaag
     1081 ctgttgaccc gctcgaagca ctcgtggagg gagtacagca cgttgagcat gaaacgggct
     1141 gccgcccaca ccgacgacga tatgtcgcac aggacgacga tcttcgcctt cttgggcggt
     1201 ctcttcctgt agacgatctc gagggggacc ccatggtacc gctcggcccg cctcagggtc
     1261 ttcttcacgt cgagcacccc cctgctgctc gcggcgtacc tcctggacac gatgtccttg
     1321 agcttcctca cgagccggtc caacgccgcg cggacctctt cgagctcccg cgcgctcagc
     1381 gcgctgaacg acctctcgcc gagctccatg agcctcttct cgtgcgtcct gtaaccgagc
     1441 gcgtcgtccg cgtaactcct cggctccctg aggagaaggg cctgggcgct gtcgatgcgg
     1501 tccgccaggt gcctcgagag ctcggccctc atccccgggt catagcgaag cctgttgccg
     1561 tctatggcgg cggccacctc ctcccgtacc ctgttgaggt tcaagaggac cctcagcttc
     1621 gaactcaggg ggccgaggct gaatttcgag cccaggttag cgctgtcgct ctcggagtgg
     1681 atctgccgta tgaggtcgag gaagcggtag gggtctcccg agaggaactc cacgatcgcc
     1741 cggtaggcct cgtcgccgtt catcgcctcc ctcagggagt cgatgatctc cgagaccccc
     1801 ttcgaaaggc cctcctcctc ggagaaatcc cccttgagct cgctgaagaa gagcctgtac
     1861 aggcggtcga accgaagctg ctcgtttctg ctcttggcga agttcgccct caaaaccgcc
     1921 ctgaactcgt cctcgtccag cacgtctacg tgctcgagct gggaagcgca gtcgatcacc
     1981 tcggatgtgg agatgcggta tcccgcggcc ctgcagcagg ccacgaactt gagcagcggg
     2041 ctcatcatgc ggtgcagcct tgcccgagga gctcggcggc ccgggcacgt acggtgtccg
     2101 cgtccgagcg gtacttgcag aggaccccaa cggtctccac gaggatctcc ggggtgatgt
     2161 cctcgacgct gagcgcaagc agcgagcgcg cccaatcgat cgtctcggag atgcagggct
     2221 tcttcttgag gtccacctcc ctgatcctcc tgaccgcatc caccaccttg tcggccagcc
     2281 gatcgggtat acccggtatc ttcacggtca cgatgcgcct ctcgaggtcc ctgtccgggt
     2341 agtcgatgta gatgtgcaca cagcgcctct tcagggcgtc gctcatgtcc cggtagttgt
     2401 tcgaggtgag caccacgaag gggatcacgc tggcccgctt cgtccctatc tccggtatgg
     2461 agacctggaa atcgctcaag agctcgagga ggaaggcctc gaactccggg tctgacttgt
     2521 ccacctcgtc gatgagaagg accaccgggt cgttcgaggt gatcgccctg aggagcggcc
     2581 tcggctggat gaaacgctcg gagaagaagg cgtcctcctg ctccgctatg cggtccaccg
     2641 cctctgcgag gctgcctgcg ccgtcgatca cgtcgccgat cttctccttg accatctggg
     2701 tgtacaggag ctgcttggcg tactcccact c
//
LOCUS       DAOSCR010000384         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24111_length_2728_cov_4.089787, whole genome shotgun sequence.
ACCESSION   DAOSCR010000384 DAOSCR010000000
VERSION     DAOSCR010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24111_length_2728_cov_4.089787"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /gene="feoB"
                     /locus_tag="PLT69_09260"
     CDS             <1..1751
                     /gene="feoB"
                     /locus_tag="PLT69_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ14665.1"
                     /translation="TYSISSVDPAEKVARDFLLSGSADVVINVLDSSILGRSLELTVQ
                     LMEMNVPMVMALNMHDEALDKGIDIDVEKLRELTGVEAYPVVAVEGKGVRVLFEAAVR
                     VARSDFKPVKPVYDRDVEECIARILSAYPERLRSKLPLDSRFVVIRLLEMDEEFERIV
                     WREDADFLSHVIKERKALAELHNWPEAGVFSSHRHAYVFDLFEAVAKVSAKQKVTLRE
                     HMDRFITNPAGGIATVLAILFLMFLASFTLGDIISGLLEQPLGMLDELAAGLPEGALQ
                     AVVSGLIDGVVSGMGIVLPYLLPLLAVMAMLEDSGLLPRIAFMVDGILHRFGMHGKAA
                     IPLILGYGCSVPAVMAARNMEYERDRIVTMLTVPFIACSARSVVILALAGKYLGAGVT
                     TAVYAANILVTLMVAFFLSRMSTQAFPGIIMDVPPLRRPYPRIVANKVWWRLREFLVF
                     AWPVIIVSSVALAFLEKVGIDGIVNRALSPVTSGLLALPEATGVTLFFGIFRKELTLA
                     MLNQALGAMDLASVLSSGQILVLTVFSMLYIPCVATLTTLWKEGGIKIVLSSILLNTT
                     VALLAAGGLSWVLRAF"
     gene            2472..>2728
                     /locus_tag="PLT69_09265"
     CDS             2472..>2728
                     /locus_tag="PLT69_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14666.1"
                     /translation="MTEATIQALNGLRDLTMVKWYIIPLLAIVFYIYTKEIKAAKQSG
                     DWNAVFAGATIFGVDFFNETWNGWVLHFTQRSAFWTTPGDTA"
BASE COUNT          484 a          876 c          848 g          520 t
ORIGIN      
        1 gcacgtactc catatcgtcc gtggacccgg cggagaaggt ggcgagggac ttcctcctgt
       61 ccggcagcgc ggacgtggtg atcaacgtgc tcgactcgtc catcctcggc cggtctctcg
      121 agctcacggt gcagctcatg gagatgaacg tgcccatggt catggccctg aacatgcacg
      181 acgaggccct ggacaagggt atcgacatcg atgtggagaa actgcgggaa ctcaccggcg
      241 tcgaggcgta cccggtggtc gccgtcgagg gcaagggcgt ccgggtcctc ttcgaggccg
      301 ccgtccgggt ggcgagaagc gacttcaagc ctgtcaaacc ggtatacgac agggacgtcg
      361 aggagtgcat cgccaggatc ctgtccgcat accccgagcg gctgagaagc aagctgcccc
      421 tggacagccg cttcgtggtc atccggctcc tcgagatgga cgaggagttc gagaggatcg
      481 tctggaggga ggacgcggac ttcctctccc acgtcatcaa ggagagaaag gcgctcgcag
      541 agctgcacaa ctggccggag gcgggggtgt tctcctccca caggcacgcc tacgtgttcg
      601 acctcttcga ggcagtggca aaggtctcgg ccaaacagaa ggtcaccctg agagaacaca
      661 tggaccgctt catcaccaac ccggcgggcg gtatcgcgac ggtgctcgcc atcctgttcc
      721 tcatgtttct cgcatcgttc acgctcgggg acatcatctc gggcttattg gaacaaccgc
      781 tcgggatgct ggatgaactg gcggcagggc tccccgaggg ggccttgcag gccgtggttt
      841 ccgggctcat cgacggggtg gtctccggga tggggatcgt gctgccctac ctcctgccac
      901 ttctggcggt catggcgatg ttggaagact ccggcctcct gccgaggatc gccttcatgg
      961 tggacggcat cctgcaccgc ttcggcatgc acgggaaggc ggccatcccc ctcatcctgg
     1021 gatacggctg cagcgtgccc gcggtcatgg ccgcccgcaa catggagtac gagcgggacc
     1081 ggatcgtgac catgctcacc gtgcccttca tcgcctgttc cgcaaggagt gtcgtgatcc
     1141 tcgcgctcgc cgggaagtac ctgggcgccg gcgtgacgac cgccgtctac gcggccaaca
     1201 tcctcgtcac cctcatggtt gcgttcttcc tctcccggat gagcacgcaa gccttccctg
     1261 gcatcatcat ggacgtcccg ccgctgagga ggccgtatcc gaggatcgtg gcgaacaagg
     1321 tctggtggag attgcgcgag ttcctcgtct tcgcctggcc cgtcatcatc gtctcgagcg
     1381 tggcgctggc cttcctcgaa aaggtgggca tagacggcat cgtcaaccgg gccctgtccc
     1441 ctgtcacatc tggcctgttg gcgctgcccg aagccaccgg cgtcaccctg ttcttcggga
     1501 tcttccgcaa ggagctcacg ctcgcgatgt tgaaccaggc gctcggcgcc atggatctcg
     1561 catccgtgct ctcgagcggg cagatactcg tcctgaccgt cttctcgatg ctctacatac
     1621 cgtgcgtggc caccctgacc accctctgga aggaaggggg catcaagatc gtgctctcgt
     1681 caatcctgct gaacaccacg gtcgccctgc tcgcggccgg tgggctgtcc tgggtcctca
     1741 gggccttctg acaatacatc cagaggcccg caggccaagg cacaaaaacg agcccggacc
     1801 gcccagcgtg agcccggcgg cgacggccgt gcgtgcccgg gccttcggcc tgatgcggct
     1861 ggatagttgg gactggtcgc gcgacggccg tgcgtgcgcg ggccttcggc ttctgtaggt
     1921 ctgccagcgc cgccaggatc gcgactgccg tgcgtgcgca ggccttcggc accgtcgagc
     1981 accagcgaac gcggaccgtc gaggccgacc ctaccctcgg ggccggtctt cctcgcgtgc
     2041 tccatctcga caaggacgat cgagcatggc cttgtcgatt ccgcagcccg acctgaagac
     2101 ccgaggcact tcccccgcag gccccaacgg ggcgtgggag gcccttacga gaaggccgat
     2161 cctccaagct cgagagacga gcattctcgc cctgtcttcc atgcgccgcg ttgacctggg
     2221 accgtcccgt gatgtcgtcc catgcgcgca catgcctttc caccgcgttc cacaggcaag
     2281 aagggtgggt gttaagagaa agtacaacgc acgggccccc ctttcccgtc atgacacacc
     2341 tctttcacct ggccggttga caaaaggagg gatctgattt attgaattat cgattctttt
     2401 gttcgattgt tcgtgaatct cgcgaccgtg cacagatgga tggaatctca ttacaaaggg
     2461 ggatcgtaac gatgacagag gccaccattc aggctttgaa cgggctcagg gacctgacca
     2521 tggtcaagtg gtacatcatc ccgctgcttg cgatcgtgtt ctacatctac acgaaagaga
     2581 tcaaggccgc caagcaaagc ggcgactgga atgcggtctt cgcgggcgcc acgatcttcg
     2641 gcgtggactt cttcaacgag acctggaacg ggtgggtgct ccacttcacg cagagaagcg
     2701 ccttctggac aacgcctggc gacacggc
//
LOCUS       DAOSCR010000385         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24174_length_2722_cov_2.854518, whole genome shotgun sequence.
ACCESSION   DAOSCR010000385 DAOSCR010000000
VERSION     DAOSCR010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24174_length_2722_cov_2.854518"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2371)
                     /locus_tag="PLT69_09270"
     CDS             complement(89..2371)
                     /locus_tag="PLT69_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14667.1"
                     /translation="MHLTGGGQEHAGGLLSRPSVQRAVVCLVLVCVVGLIYGQVVHHE
                     FLLFDDNHYVNENPMVRRGLSMEGLRYAFGFHSKTYWHPVTWLSHMLEVELFGMDSGM
                     HHLVNAFLHAVNACLLFAFLHTATGAFWPSAAAALLFAVHPLNVDSVAWLAERKNILS
                     STFWMLCLLAYPWYVRKRTAARYTALALLFVAGLLAKPMLVTLPFVLLLLDYWPLGRI
                     SLGRVQERPGKKNGRGAFRPEGEPPGRLLAEKVPLFVLSGASIVVSSVSLRVGGMMVE
                     SSLTPMVLRIQNAVVSYSYYIVKLLWPSGLTFYYPFPESALPAWQVIGSLTLVASVSA
                     LVLAYARRAPYLVVGWLWYLGTLVPVLGIVQGGLWPQIAERFMYIPAIGLFVCACFGA
                     RDLAARSRAAVPAIACAAAVVLVVLSGLSWKQVSYWKDSYIMDLRAIEVNPKNYVALV
                     NLAVYLCNKNRYEEALPYLKKALEAHPTDNVVLSTLARVYRGLGRQEEAMRYLEEAAR
                     YDRQGVEARYDLGLYLAEKGDVDRAISQFEQVLGREPHHVLAHYNLGVLMAKKGRLDE
                     SERHLRTCLELKPDEVDCLNALGMLLVTRGKPSEAEALFRKALTVDPRNADAQNNLRA
                     LAGKKQPGGAGAVPDDSESIRRRLEKEPGNARLLQLLAVRLAGKGDLDGALDALGSYM
                     KLRPDDPAGYYNAACVHAKKGEVDKALDLLGMALDKGFSDWRLIETDPDLDHARTSPR
                     FRRLLEAHRGAGGADGAPRG"
     gene            complement(2382..>2722)
                     /locus_tag="PLT69_09275"
     CDS             complement(2382..>2722)
                     /locus_tag="PLT69_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ14668.1"
                     /translation="AMRYLNMVGNKFFSLAFTWLLGQPIKDTLCGTKVLTRSDYARIA
                     ANRNYFGDFDPFGDFDLLFGAAKQNLKIVEIPVRYRERTYGTTNIDRWRHGLLLLRMT
                     LFAMRRIKFF"
BASE COUNT          470 a          889 c          911 g          452 t
ORIGIN      
        1 gagcatggtc gttctcgggt cgtcgagggg gatgtctctc gtgagcggat gggcgagagc
       61 ccgcctgatc cacgaggcga gagacccttc agccgcgcgg cgcgccatcg gcaccccctg
      121 cgcccctgtg cgcctccaga agcctcctga agcgcggcga cgtccttgcg tggtcgaggt
      181 ccgggtcggt ctcgatgagc ctccagtctg aaaacccctt gtcgagagcc atgcccagca
      241 ggtccagcgc cttgtccacc tctcccttct tcgcgtgcac gcaggccgcg ttgtagtacc
      301 ccgcggggtc gtccggcctc agcttcatgt aactcccgag cgcatcgaga gccccgtcga
      361 ggtccccctt gcccgcgagc ctgacggcga gcagctggag caggcgggcg ttgccgggct
      421 ccttctcgag ccttcgcctg atggactcac tgtcgtccgg cacggccccg gccccacccg
      481 gctgtttctt cccggcgagc gcgcggaggt tgttttgggc gtcggcgttc cgcggatcga
      541 ccgtgagggc cttcctgaag agggcctcgg cctccgatgg tttccccctt gtcaccagca
      601 gcatgcccag ggcgttgaga caatccacct cgtcgggctt cagctcgagg catgtcctca
      661 ggtgcctctc cgactcgtcg agacggccct tcttcgccat gagcacgccg aggttgtagt
      721 gggccaacac gtggtggggt tccctcccca gcacttgctc gaactgggag atcgccctgt
      781 ccacgtcgcc cttctcggcg aggtacaggc cgaggtcgta gcgcgcctcc acgccctggc
      841 ggtcgtacct ggccgcctcc tcgaggtatc tcatggcctc ctcctgcctg ccgagccccc
      901 tgtacacgcg cgcgagcgtg ctcagcacga cgttgtcagt gggatgggcc tcgagggcct
      961 tcttcaggta gggcagagcc tcctcgtaac ggttcttgtt gcagaggtag acggcgaggt
     1021 tcacgagggc cacgtagttc ttcgggttca cctcgatggc cctgaggtcc atgatgtagc
     1081 tgtccttcca gtaggagacc tgcttccagg aaaggcccga cagcacgacc aggaccaccg
     1141 cggcggcaca ggcgatcgcg gggacggcgg cgcgtgagcg cgcggcgagg tcgcgcgccc
     1201 cgaagcacgc gcacacgaag aggccgatcg cggggatgta catgaacctc tccgctatct
     1261 ggggccacaa cccaccttgg acgatcccga gcacgggcac gagggtcccg aggtaccaca
     1321 gccaacccac gacgagatac ggggcgcgcc tggcgtacgc gaggaccagg gcgctcaccg
     1381 acgcgacgag ggtgagggag ccgatcacct gccaggcggg cagggcgctc tcggggaacg
     1441 ggtagtagaa cgtcaggccg gagggccaca agagcttgac gatgtagtac gagtacgaga
     1501 ccaccgcgtt ctggatgcgc agcaccatgg gcgtgaggga tgactcgacc atcatgccac
     1561 ccaccctgag agagacggac gagaccacga tggaggcgcc ggacagcacg aacagcggga
     1621 ccttctcggc cagcaggcgg ccgggtggct ccccctcggg cctgaaggcc cccctgccgt
     1681 ttttcttccc aggcctttcc tggacgcgcc cgaggctgat acggccgagc ggccagtagt
     1741 ccagcaggag gagcacgaaa ggcagggtca cgagcatggg cttggcgagc aggccggcca
     1801 cgaagaggag ggcgagcgcc gtgtagcgcg cggcggtgcg tttcctcacg taccaagggt
     1861 aggcgaggag gcacagcatc cagaacgtgc tgctcaggat gttcttgcgc tccgcgagcc
     1921 acgccaccga gtccacgttc agcgggtgca cggcgaacag caaggccgcc gccgcgctgg
     1981 gccagaaggc gccggtcgcg gtgtgcagga aggcgaagag caggcaggcg ttgaccgcat
     2041 ggaggaaggc gttcaccagg tggtgcatgc cgctgtccat gccgaacagc tccacctcga
     2101 gcatgtgcga cagccacgtc accgggtgcc agtacgtctt gctgtggaaa ccgaaggcgt
     2161 acctgaggcc ctccatgctc aggccccgcc tgaccatggg gttctcgttc acgtagtggt
     2221 tgtcgtcgaa gaggaggaac tcgtggtgca cgacctgccc gtagatgaga cccaccacgc
     2281 acacgagcac gaggcagacc acggccctct gtaccgaggg tctgctgaga agcccgcctg
     2341 cgtgttcctg accgccgccc gtgagatgca caacttaccc cctagaagaa cttgatgcgc
     2401 ctcatggcga agagggtcat gcgcaacagc aacagcccgt ggcgccagcg gtcgatgttc
     2461 gtggtgccgt aggtgcgctc ccggtagcga accgggatct ccacgatctt gaggttctgc
     2521 ttggccgcgc cgaagagcag gtcgaagtcg ccgaaggggt cgaagtcgcc gaagtagttc
     2581 ctgttggccg ctatcctcgc gtagtccgag cgcgtcagca ccttggtccc gcaaagcgtg
     2641 tccttgatgg gctggcccag cagccacgtg aaggcgaggg agaagaattt gttgcccacc
     2701 atgttgaggt agcgcatggc ct
//
LOCUS       DAOSCR010000386         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24538_length_2696_cov_2.719803, whole genome shotgun sequence.
ACCESSION   DAOSCR010000386 DAOSCR010000000
VERSION     DAOSCR010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24538_length_2696_cov_2.719803"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLT69_09280"
     CDS             <1..591
                     /locus_tag="PLT69_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ14669.1"
                     /translation="AVLGLKDPKAVLQRIRASTGREIFEIPTLPPSVPGIRLFRALKD
                     AFLARGGHLYWGNAVASVETRSGQVEAITMAGPCRPSRAHARAFILATGSFVGGGLFA
                     QRGSVRETVFDLDVKVPGPRRDWFLNDFFSPGHPVEASGIEVDSSFRPLGCGLKNLFA
                     CGSILAHGEIMRHRCGHGLAIATGSAAARACERLLS"
     gene            588..1844
                     /locus_tag="PLT69_09285"
     CDS             588..1844
                     /locus_tag="PLT69_09285"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HOJ14670.1"
                     /translation="MSFEHCMRCTVCVENCPVAKVEPRFPGPKQAGPDAQRFRLDGEK
                     SVDAWVNYCSHCMRCQVACPYGVEVADIILKARLKYSKEHFSPLSSHLFANAYHLSKM
                     GSLAAPVMNRITPLPVFKQAMGLLGVSDVPDLPEFRFLSLERTRRSKGRGRRVVFFHG
                     CHLNFNRPDIGRKAIDLLVAFGCRVAIPRQQCCGLPALGNGDLEMARKFALKNASMLT
                     RYVNRGFDVVYSCPSCGLTLTREYPGILDVPGARKVAENTYHIFEYLLPLVQDRLDAK
                     GLRSIGRVEASIAYHVPCHLRALGIGYPSVGLFEMIDGLSFTVLDNDCCGLSGTYGFK
                     KRNRETAAKLGAIAAGAVRETGCELVVSDCGACRIQLGHYAAMPAMDPIEIVADAVSS
                     GPLANKRRTLAERVASLFPSIKGGTP"
     gene            1841..2209
                     /locus_tag="PLT69_09290"
     CDS             1841..2209
                     /locus_tag="PLT69_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14671.1"
                     /translation="MRKIVLFGTLCLILATAPATSMAASSSLEVVLVDSLWGAAIGTL
                     AGAATLAFMDHPKDHTERIYQGAAVGLFLGMAFGVYELSPMFSSYERPDGTRVNVWGL
                     ACTVGLGPGPRPSRAGLWSW"
     gene            complement(2175..>2696)
                     /locus_tag="PLT69_09295"
     CDS             complement(2175..>2696)
                     /locus_tag="PLT69_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HOJ14672.1"
                     /translation="KVEDGEELSGAREHALGFSSKVLVEQAVERALEIEVAVLGNNEP
                     KASVCGQIIPKAAFYDFNAKYVDGTSELVIPARIPKALAQDIQYAAVDAFVAIGGSGM
                     ARVDFLVSGAAFYLNEINTIPGFTSISMYPKLWEASGIPYTHLITKLIELAIARHEEK
                     ASLTRTIDLLVKA"
BASE COUNT          442 a          884 c          874 g          496 t
ORIGIN      
        1 gccgtgctcg gcttgaaaga cccgaaggcc gttctccagc gtatacgggc gtccacgggc
       61 agggagatct tcgagatacc gaccctgccc ccatcggtcc ccgggatcag gctgttcagg
      121 gcgctcaagg acgccttcct ggcacgcggc ggccacctct actggggcaa cgccgtggcg
      181 agcgtggaga cgaggtccgg acaggtggag gcgatcacca tggccgggcc ctgcaggccc
      241 tcgcgtgccc atgcacgcgc attcatcctc gccacggggt ctttcgtggg cggggggctg
      301 ttcgcccaga ggggctcggt gagggagacc gttttcgacc tcgacgtgaa ggtgccgggc
      361 ccgcgcaggg actggttcct gaacgacttc ttctcgccgg gccaccccgt ggaggcctca
      421 ggcatcgagg tggactcgtc gttcaggcct cttgggtgcg gtctgaagaa cctgttcgcc
      481 tgcgggagca tccttgccca cggcgagatc atgaggcacc gctgcggaca cggcctcgcc
      541 attgcgacgg gctcggccgc ggcccgggcg tgcgagaggt tgctgtcatg agcttcgagc
      601 actgcatgcg gtgtaccgta tgtgtggaga actgcccggt ggcaaaggtc gagccgcgct
      661 ttcccggccc caaacaggcc gggcccgacg cccaaaggtt ccgcctcgac ggggagaagt
      721 cggtcgatgc gtgggtgaac tattgctccc actgcatgag gtgccaggtg gcatgcccct
      781 acggtgtcga ggtggcggac atcatcctca aggcccggct caaatacagc aaggaacact
      841 tcagcccgct gagctcgcac ctgttcgcca acgcgtacca cctctcgaag atggggtcgc
      901 tggccgcacc ggtgatgaac aggatcaccc ccctgcctgt gttcaaacag gctatgggcc
      961 tgctgggggt ctccgatgtc ccggacctgc ccgagttcag gttcctctcg ctcgagcgga
     1021 caaggagatc caagggacgc ggcaggaggg tcgtgttctt ccacggttgc cacctgaact
     1081 tcaacaggcc tgacatcggc aggaaggcga tcgacctgct tgtcgccttc ggctgccgtg
     1141 tggcgatccc ccgccagcag tgttgcggcc tcccggcgct gggaaacggg gacctcgaga
     1201 tggcgaggaa gttcgccctc aagaacgcgt ccatgctcac caggtacgtg aaccgggggt
     1261 tcgacgtcgt gtactcctgc ccctcctgcg gtctcacgct caccagggaa taccccggga
     1321 tcctcgacgt gcccggcgcc aggaaggtag ccgagaacac gtaccacatc ttcgaatacc
     1381 tcctcccgtt ggtacaggac aggctggacg ccaaggggtt gcgatcgatc gggagggtcg
     1441 aggcgtccat tgcataccat gtcccgtgcc acctgcgcgc cctcgggatc gggtacccgt
     1501 ccgtgggcct cttcgagatg atagacggcc tctctttcac ggtactcgac aacgactgct
     1561 gcggcctctc aggcacctac gggttcaaga agcgcaaccg agaaaccgcg gcgaaactcg
     1621 gtgccatcgc tgcgggcgcg gtgagagaga cgggctgtga actcgtggtc tcagactgcg
     1681 gcgcctgccg catccagctc ggccactacg cggccatgcc ggccatggac cccatcgaga
     1741 tcgttgcgga cgccgtgtcc agcgggcccc tggcgaataa gcgccgcact cttgcagaga
     1801 gggtcgcatc cctcttcccg tcgatcaaag gaggaacccc atgaggaaga tcgtcctttt
     1861 cggcaccctg tgtctcatcc tcgcgacggc ccccgccacc tcgatggccg catcgtcgtc
     1921 cctggaagtg gtcctcgtgg actcgctctg gggcgcggcc atcgggacgc tcgcaggggc
     1981 ggcgacgctc gcgttcatgg atcacccgaa ggatcacacc gagcgcatct accagggtgc
     2041 ggcggtgggc ctttttctgg ggatggcctt cggcgtctac gagctctccc ccatgttctc
     2101 atcgtacgag aggcccgacg gcacgcgggt gaacgtctgg ggcctcgcct gcacggtggg
     2161 cctcgggccg gggcctaggc cttcacgagc aggtctatgg tcctggtgag agaggccttc
     2221 tcttcgtgcc tggcgatcgc gagctctatg agcttcgtga tcaggtgagt gtacgggatg
     2281 ccggaggcct cccagagttt cgggtacatc gatatgctcg tgaaccccgg gatcgtgttg
     2341 atctcgttga ggtagaacgc cgctcccgag accaggaagt ccaccctcgc catgcctgag
     2401 ccgccgatgg ccacgaaggc gtccaccgcg gcgtactgga tatcctgggc cagcgccttc
     2461 ggtatgcgcg ccgggatgac gagctcgctc gtgccgtcca cgtacttggc gttgaagtcg
     2521 tagaacgccg ccttcgggat gatctggccg cagaccgatg ccttgggctc gttgttgccg
     2581 agcaccgcga cctctatctc cagagcccgt tccaccgcct gctccacgag gaccttggac
     2641 gagaacccga gggcatgctc tctggccccg ctcagctcct caccgtcttc gacctt
//
LOCUS       DAOSCR010000387         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24560_length_2693_cov_3.854056, whole genome shotgun sequence.
ACCESSION   DAOSCR010000387 DAOSCR010000000
VERSION     DAOSCR010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24560_length_2693_cov_3.854056"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLT69_09300"
     CDS             <1..639
                     /locus_tag="PLT69_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ14673.1"
                     /translation="VTYVGPLSALPWTYFGTMELGQILDPRGPHVGASGSPTYFARRG
                     LEVFPGHLARMGVPEQAVKRILQGREGAGRGLNVGRLLRYSHRWFTILGSLGVCARAQ
                     VNRFYSAGLLARLYEAVTGIPTDAEKLAERADRSWTLLALANAGAGRGLTPGEVPRAW
                     YGGEGFRDYVDGTALSLEDIGGMIADYYDEQGWDPVTGRPSPARLQELGLAE"
     gene            complement(655..1314)
                     /gene="phoU"
                     /locus_tag="PLT69_09305"
     CDS             complement(655..1314)
                     /gene="phoU"
                     /locus_tag="PLT69_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOJ14674.1"
                     /translation="MLEESKIVQLKKEITEFATLVESMVDKSVSGLEERRVDLLMEVI
                     ERDEPRANDHEMDIDERCVSIIAQHQPAGRPLRTVLMVSHINSTLERVADHAVTIAES
                     ALYLISRPPVKQLIDIPRMAQMVEIMIKDSITSFLNDDAELAKGVCERDDTVDGLRDQ
                     IVRELITYMTSDPATIERALHLMKITSNLERIADLATNICEDVIYMVKGKVIKHHKET
                     L"
     gene            complement(1324..2085)
                     /gene="pstB"
                     /locus_tag="PLT69_09310"
     CDS             complement(1324..2085)
                     /gene="pstB"
                     /locus_tag="PLT69_09310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOJ14675.1"
                     /translation="MALEAKIRASDVHFYYGQTRALTGISMEIHERCVTALIGPSGCG
                     KSTFIRLLNRMNDLVPNTRVEGRILLDGKDIYHPKTDVVELRRKVGMVFQKPNPFPKS
                     IYENMAYGLLVGGVKDRGFIEHRVITALKKAALYDDVKDRLHDSALKLSGGQQQRLCI
                     ARCLVVEPEVILFDEPCASLDPISTRKIEELILELKKDYTIVIVTHNMQQAARVSDYT
                     AFMYLGELVEYDRTDKIFTVPKERMTEEYLSGKFG"
     gene            complement(2075..>2693)
                     /gene="pstA"
                     /locus_tag="PLT69_09315"
     CDS             complement(2075..>2693)
                     /gene="pstA"
                     /locus_tag="PLT69_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOJ14676.1"
                     /translation="LGLASAIYLCEYTPKSYLVNLIRLSINNLAGVPSVVFGLFGFAV
                     FVKGFGFGVSILSGSLTLGIMALPGIISASQEAILAVPQSYREASLALGASHWQTIRE
                     IVLPSALPGILTGMILTIGRVAGETAPILFTAATFYTRGYPDSIFSEVMALPYHIYAL
                     MTEGTRPELQTPIAYGCSLILLCLVLAVSALAIVLRQRQRFSYGS"
BASE COUNT          450 a          823 c          904 g          516 t
ORIGIN      
        1 gtcacctacg tgggtccgct gtcagcgctt ccctggacgt atttcgggac catggagctc
       61 ggacagatcc tcgaccccag gggcccgcac gtcggggcct ccgggtcgcc cacctacttc
      121 gcgaggcgcg ggctcgaggt cttccccggc cacctggcgc gcatgggggt ccctgaacag
      181 gccgtgaaga ggatccttca ggggcgggaa ggagccgggc gcggcctgaa cgtgggcagg
      241 ctgctcaggt actcccaccg ctggttcacg atactcggaa gcctgggcgt ctgcgcgagg
      301 gcccaggtga acaggtttta cagcgcgggc ctcctggcga ggctctacga ggcggtcacc
      361 gggatcccga ccgacgcgga gaagctggcg gagcgtgcgg acaggtcctg gaccctcctc
      421 gccctcgcga acgcaggggc gggccgcggc ctcactccag gggaggtccc gcgcgcgtgg
      481 tacggcgggg aaggcttcag ggactacgtc gacggtaccg cgctcagcct cgaggacata
      541 ggcggaatga tcgccgacta ctacgacgag cagggctggg accctgtcac cggcagaccc
      601 tctccagcca ggctccagga gctcggcctc gcagagtgac ctcccccggc cgcctcagag
      661 ggtctccttg tgatgcttga tcaccttgcc cttcaccatg tagatcacgt cctcgcagat
      721 gttggtggcc agatcggcga tacgctccag gttgctcgtt atcttcatga ggtggagggc
      781 cctctcgatg gtggcggggt ctgaggtcat gtaggtgatg agttcgcgca cgatctggtc
      841 cctcaggccg tcgacggtgt cgtcccgctc gcacaccccc tttgcgagct cggcgtcgtc
      901 gttgaggaac gaggtgatgc tgtccttgat catgatctcc accatctgcg ccatccgggg
      961 gatatcgatg agctgcttca ccggagggcg cgagatgagg tacagggcgc tttccgctat
     1021 ggtgacggcg tggtcggcta cccgttccag ggtgctgttg atgtgggaca ccatgaggac
     1081 cgttctgagg ggcctccccg ccggctggtg ctgcgcgatg atggagacgc agcgctcgtc
     1141 gatgtccatc tcatggtcgt tcgccctggg ctcgtcgcgc tcgatgacct ccatgagcag
     1201 atccaccctt cgttcctcga gccccgagac gctcttgtcg accatggact cgacgagcgt
     1261 cgcgaactcc gtgatctcct tcttcaactg aacgatcttc gactcttcca gcatacgatg
     1321 acctcaaccg aactttccgc tcaggtattc ctctgtcatg cgctccttcg ggacggtaaa
     1381 gatcttgtcc gtccggtcgt actcgacgag ctcccccagg tacatgaagg ccgtgtagtc
     1441 cgagacgcgc gcagcctgct gcatgttgtg ggtgacgatc acgatggtgt agtccttctt
     1501 gagttcgagg atgagttcct cgatcttgcg ggtggagatg gggtcgaggc tcgcgcaggg
     1561 ctcgtcgaag aggatcacct ccggttccac caccaggcac ctggcgatgc agagccgttg
     1621 ctgctggcct cccgagagct tgagcgcgct gtcgtggagg cggtccttga cgtcgtcgta
     1681 cagggcggcc ttcttgaggg cggttatcac gcggtgctcg atgaaccccc tgtccttgac
     1741 gcccccgacg agaagcccgt aggccatgtt ctcgtagatg ctcttgggga aggggttcgg
     1801 tttctggaac accatgccga ccttcctcct gagctccacc acgtcggtct tcgggtggta
     1861 gatgtccttt ccgtccagga gtatccggcc ctcaaccctg gtgttgggca cgaggtcgtt
     1921 catccggttg aggagcctga tgaaggtgga cttcccgcac ccggaggggc cgatgagcgc
     1981 ggtgacgcac ctctcgtgga tctccatgct gatcccggtg agggccctgg tctggccgta
     2041 gtagaaatgc acgtcggatg cgcggatctt ggcctcaaga gccataggag aacctctgtc
     2101 tctgtctcag cacgatggcg agggcggaca cggcgagcac caggcacagg aggatgagag
     2161 agcagccgta ggcgatgggg gtctgcagct caggcctggt cccctcggtc atgagcgcat
     2221 agatgtggta cggcagggcc atgacctcgg agaagatcga gtccgggtac cccctggtgt
     2281 agaacgtcgc cgccgtgaag aggatgggcg cggtctcgcc cgccaccctc ccgatggtca
     2341 atatcatgcc cgtgaggatg cccggcaggg ccgaggggag gacgatttcc ctgatggtct
     2401 gccagtggga cgccccgagg gcgagggacg cctcccggta ggactggggg accgccagga
     2461 tggcttcctg ggaggccgat atgatcccgg gcagggccat gatgccgagt gtgaggctgc
     2521 ccgagaggat ggagaccccg aacccgaaac ccttgacgaa gaccgcgaag ccgaagagcc
     2581 cgaagaccac cgagggcacc cctgcaaggt tgttgatgga cagccggatg aggttcacca
     2641 ggtacgactt cggcgtgtac tcgcacaggt agatggcgct ggcgagcccc agg
//
LOCUS       DAOSCR010000388         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24670_length_2684_cov_3.348041, whole genome shotgun sequence.
ACCESSION   DAOSCR010000388 DAOSCR010000000
VERSION     DAOSCR010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24670_length_2684_cov_3.348041"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLT69_09320"
     CDS             <1..223
                     /locus_tag="PLT69_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14677.1"
                     /translation="YIARSPDHADEYVPLLQKMGVEVYAGDPLALQEFWPDVLAPHLD
                     VEKILKSRRFDYVILSFWHIARHYLPLVR"
     gene            257..>2684
                     /locus_tag="PLT69_09325"
     CDS             257..>2684
                     /locus_tag="PLT69_09325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ14678.1"
                     /translation="MDIHFLREIREAEMKRDRALLEKALKNKEQELAVYQEGDRIWVV
                     TEDDKKVIQGLVNKPIDVVPNIHKEVPWTKEFEQTKDLLFVGNFNHKPNIDAVLFLHK
                     KIFPVIRKGLPGVRVYIVGNNPPAQIRRLNSEDFIVTGYVEDLEPYLKDARISISPLT
                     YGAGMKGKVGEALSWGLPVVTTTIGAEGMDLEDGVTAMIADDPKEFARKVIELYLDRD
                     SWTRLSKNGKKHVEQRWSPSAIQKRIESSLLHMKGFTGGHVSAVLMGSLPNTHIREAL
                     DALDSFIDAPGDTVVVASDLHEGTSMALLEMQHDPLRPRKVKVLATRGDRSVKLWNRA
                     MAVCEGELAVLCRRLDPADAKVIRSLVSYALEHPQADVVTPRMPRLEDSPAFPGAAGR
                     ATRPLPFMIVRHTAFEAVGGLDSRIHDDHLAWCDYVMRASERGLQAACPKEPQEEAAQ
                     TAASEGATPGTQAESDWRYFSEKWGLSEETLKAAAGSTLALQSTHAAGDLFCSFGDEP
                     VPGSPSHIGDDDGRPVDDQEEPLPTGLTSIIVLCFNGLDYTRQCLESVEAFTPEPHEI
                     ILVDNGSTDGTRAFLEEFAKTHEHVRLVLNEENLGYAAGNNQAIRLAEGDYVVLLNND
                     VVVTEGWLRGMIRHMERDKDVGMVGPVSNSVSGRQLVHGASYGRDMQRMHEFASQVRK
                     RNAHRAEPFMRLVGFCLLVKKEVLDIIGGLDEGYLTGNYEDDDLCIRSVAAGYKNLIA
                     HDVFVHHYGSMTFKVNSLDHRRTMDANLAYFLKKWEGVVSLVSDDVYRLHLDKAAQVS
                     LLNRWGEAAFQ"
BASE COUNT          550 a          868 c          837 g          429 t
ORIGIN      
        1 ctacatagcc cgcagcccgg accacgccga tgagtacgtg ccgctcctgc agaagatggg
       61 cgtcgaggtc tacgcgggag accccctggc gctccaggaa ttctggccgg acgtattggc
      121 ccctcatctc gacgtggaga agatactgaa gtcgaggcgc ttcgactacg tgatcctctc
      181 cttctggcac atcgccaggc actacctccc cctcgtgagg tagttcagcc cgtcttcgcg
      241 cgtcatcatc gacacggtgg acatccactt cctcagggag atcagggagg ccgagatgaa
      301 gagggacagg gcgctcttgg agaaggcgct gaaaaacaag gaacaagagc tcgccgtcta
      361 ccaggaggga gacaggatct gggtcgtgac cgaggacgac aagaaggtca tccaggggct
      421 cgtgaacaag cccatcgacg tcgtcccgaa catccacaag gaggttccgt ggaccaagga
      481 gttcgagcag accaaggacc tgctcttcgt gggcaacttc aaccacaagc ccaacatcga
      541 cgcggtcctg ttcctccaca agaagatctt ccccgtcatc aggaagggtc tccccggcgt
      601 cagggtgtac atcgtgggga acaaccctcc cgcccagatc cggcgcctca acagcgagga
      661 cttcatcgtg acggggtatg tcgaggacct cgaaccctac ctgaaagacg cccgcatctc
      721 catcagcccc ctcacctatg gtgcgggcat gaagggaaag gtgggcgagg cgttgtcctg
      781 ggggcttccc gtggtgacca ccacgatagg ggccgagggc atggacctcg aagacggcgt
      841 aaccgccatg atagccgacg acccgaagga attcgccagg aaggtcatcg agctctacct
      901 cgaccgggat tcgtggacgc gtctctcgaa aaacggaaag aagcacgtcg aacagaggtg
      961 gtccccctcc gccatccaga agaggataga atcctcgctc cttcacatga aggggttcac
     1021 cggggggcac gtctcggccg tgctcatggg ctcgctcccc aacacgcaca tacgcgaggc
     1081 cctcgatgcg ctggattcgt tcatagacgc acccggcgac accgtggtgg tcgcgagcga
     1141 tctccacgag ggcacatcca tggccctgct cgagatgcag cacgaccctc tcaggccaag
     1201 gaaggtaaag gtactggcga caagggggga ccgcagcgtc aagctgtgga acagggccat
     1261 ggccgtgtgc gagggcgagc tcgccgtcct ttgccgccgc ctcgacccgg cggatgcgaa
     1321 ggtgatccgc tcgctggtct cctacgccct cgaacacccc caggcggacg tcgtcacgcc
     1381 ccgcatgcca cggctcgagg attcccccgc ctttcccggc gccgcaggcc gggcgacaag
     1441 gcccctcccc ttcatgatcg tgcgccacac ggccttcgag gcggtgggcg gcctcgactc
     1501 caggatccac gacgaccacc tcgcgtggtg cgattacgtc atgcgagcct ccgagcgggg
     1561 gctccaggcc gcgtgcccga aggagccgca ggaagaagcg gcccagaccg ccgcgagcga
     1621 aggcgcgacc cccggcacac aggccgaatc cgactggaga tacttctccg agaagtgggg
     1681 cctttccgag gaaaccctga aggccgctgc cggctcgacg ctcgccttgc agtccactca
     1741 tgcggccggt gatctcttct gctccttcgg cgacgagccc gtcccaggca gtccgtccca
     1801 catcggggac gacgacggac ggcccgtgga cgaccaggag gagcctctgc ccacgggcct
     1861 cacctccatc atcgtactgt gcttcaacgg cctggattac accaggcagt gcctcgagag
     1921 cgtggaggcg ttcacgcccg agccgcacga gatcattctc gtggacaatg gatccaccga
     1981 cgggacgagg gctttcctgg aggagttcgc caagacccac gagcatgtca ggctcgtcct
     2041 gaacgaggaa aatctcgggt atgccgcggg caacaaccag gccatccggc tggccgaggg
     2101 cgactacgtg gtgctgctca acaacgacgt ggtcgtgacc gagggctggc tccgggggat
     2161 gatccggcac atggaaagag acaaggacgt gggcatggtg ggcccggtct ccaactccgt
     2221 ctcgggcagg cagctcgtcc acggtgcgtc ctatggccgg gacatgcagc ggatgcacga
     2281 gttcgcctca caggtcagga agaggaacgc acacagggcg gaacccttca tgaggctcgt
     2341 gggtttctgc ctcctcgtca agaaagaggt gctcgacatc atcggcggcc tcgacgaagg
     2401 gtatctcacg ggcaactacg aggacgacga cctctgcatc aggtccgtgg cggccggcta
     2461 caagaacctc atcgcacacg acgtgttcgt ccaccactac ggatccatga cgttcaaggt
     2521 gaactccctc gaccacaggc gcaccatgga tgccaatctc gcgtacttcc tgaagaagtg
     2581 ggaaggggtc gtcagcctcg tgagcgatga cgtctaccgg ctgcacctgg acaaggccgc
     2641 ccaggtttcc ctcctcaacc ggtgggggga ggcggcgttt cagg
//
LOCUS       DAOSCR010000389         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24728_length_2679_cov_2.963415, whole genome shotgun sequence.
ACCESSION   DAOSCR010000389 DAOSCR010000000
VERSION     DAOSCR010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24728_length_2679_cov_2.963415"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLT69_09330"
     CDS             <1..320
                     /locus_tag="PLT69_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14679.1"
                     /translation="SFFHALERLMTRTSTAFPDHEIESLEECAELCLAVGLRLREMGE
                     NNLAMRMAESAVRLDPSCASAFLLQADVYRDRGETARMAACLEEALKHGADRREVLER
                     LQP"
     gene            complement(494..958)
                     /locus_tag="PLT69_09335"
     CDS             complement(494..958)
                     /locus_tag="PLT69_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14680.1"
                     /translation="MGRTAKGLYASFILGIVCALCLLPAPSMATDDDDNAVLRENLLP
                     AQQGDAKAQVFVGYLYETGQGVRQDYAKAAKWYEKAAWQGNATAQMQLGDMYLKGKGV
                     PQNYVMAYMWLELASLQGSTQASTLRKDVSSRMTPVQLAEAKKLVRNWKAKK"
     gene            1495..>2679
                     /locus_tag="PLT69_09340"
     CDS             1495..>2679
                     /locus_tag="PLT69_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOJ14681.1"
                     /translation="MGSGRMAVFVAACIVCSSWAAQAARIDRAVIYDDQAVISFTQAI
                     KGTLDIEAPACVVAGSLVVRPLDGARVESASLTPARVESGAARSLREELAVVQTALSL
                     KKRELTSTDRQIDTIFQVLASKDANPGDTRRLLEVLGFVDERVRALNKKAVETQSAIA
                     ELETRAKDLKERLASLGDGPGWVIRITGSGRVNVSYAVLQATFKPEYRIHALPDSSSL
                     SIDYLATISQATGMDWEVGELLVATGRPSHGIHAPEPVPWHVGSAKRVFGKASAEAQD
                     ISPLTAPAPAGALTPDTEPTTVTTVIGVAKDVRLPGDGTPVTAAVAGIRLDAAFTAIV
                     VPRLDQRAYLRGETTLAGAIPVVEGPYAAFVDGVYAGSGMMGRFAPGQKMTVDFGVQE
                     GIL"
BASE COUNT          467 a          899 c          853 g          460 t
ORIGIN      
        1 actcgttctt tcatgccctg gaacgactca tgacaagaac atccaccgca ttccccgacc
       61 acgagatcga atccctggag gagtgcgccg agctctgcct ggctgtcggc ctcaggctga
      121 gggagatggg cgagaacaac ctcgccatgc gcatggccga gtcggccgta cgcctggacc
      181 cttcatgcgc ctccgccttc ctcctccagg cggatgtgta ccgggacagg ggcgagacgg
      241 cgcgaatggc cgcctgcctc gaggaggcgc tcaaacatgg cgccgaccgc cgcgaggtcc
      301 tcgagcgcct gcaaccctga atgccgggga tcgaggatgt ctcggccctg agacactctc
      361 gagacaggtc gttcgagggt tacggtgaac atgcggagaa acacggcccg gaagaacgag
      421 gacgaatcgc gtccgccaag ggcgtcaaca ggcggcccgg ttgccgggat gaccggcaag
      481 acccagccct cgcctacttt ttcgccttcc agttcctgac gagtttcttg gcctctgcga
      541 gctgtacagg ggtcatcctc gacgagacgt ccttcctgag ggtcgaggcc tgggtgctgc
      601 cctggaggga ggcgagctcc agccacatgt aggccatcac gtagttctgc ggcaccccct
      661 tccccttcag gtacatgtcc cccagctgca tctgggccgt tgcgttaccc tgccaggcgg
      721 ccttctcgta ccacttcgca gccttggcat agtcctgcct gacaccctgg ccggtctcgt
      781 agaggtagcc aacgaagacc tgggccttcg cgtctccctg ctgggcgggc aggaggttct
      841 ccctgagcac ggcgttgtcg tcgtcgtcgg ttgccatcga aggcgccggg aggagacaga
      901 gtgcacagac gatacccagg ataaacgagg cgtacaggcc tttcgcagtc ctccccatac
      961 attcccccct agactgcctg gtggaataac attctcgtaa tcccggcacc accttacctg
     1021 cattcaagcg gagagtcaat atggcggggc aggcccttgg gcactcgtga cacacttttc
     1081 tccccccttc ctccgttttg ttgggcgggc tgaagaagct ccgcagcatg caggtatcca
     1141 gcgatgtcgt ctacgacctg gggtcatggc tctttctgcc gggcaggcac gaggccatcg
     1201 cacaggcggg ctcgcagcct gtgcgcggtt ggacgtcagg ggtagcggca ccacggcaag
     1261 gcgccggtgg gcgcaagaga gacgacccgg tgtcccccac cccggcatac cctttccagg
     1321 ctcttgcgtg ccgctttccc acgtacccgc ctcctcacgc cctctcctcc cggtatcccc
     1381 ccgcggctca ccgggtccgt gcgccagagt tgtcgttgtg gacgacgcgc agagcatgta
     1441 ggatgatcac atcgagcaga catgctcctc aatcccctac ggaggtcttc aggcatgggt
     1501 agtggccgta tggctgtgtt cgtggccgca tgcatcgtgt gctcctcgtg ggctgcgcag
     1561 gccgcccgga tcgacagggc ggtgatctac gacgaccagg ccgtcatatc cttcacccag
     1621 gcgatcaagg gaaccctgga catcgaggcg ccggcgtgcg tcgtggccgg gtccctcgtc
     1681 gtgaggcccc tcgatggtgc gcgggtggaa tccgcatccc ttacacccgc gcgtgtggag
     1741 tccggcgcag ccaggtccct cagggaggaa ctcgcggtgg tccagacggc cctctcgctc
     1801 aagaaacgcg agctgacgag cacggacagg cagatcgaca cgatcttcca ggtgctcgcc
     1861 tccaaggacg cgaacccggg ggacaccagg cgcctcctcg aggtcctcgg tttcgtggat
     1921 gagcgcgtgc gggcattgaa caagaaggcg gtcgagaccc agtccgccat cgcagagctc
     1981 gagacacgcg ccaaagacct caaggagcga ctcgcctcgc tgggcgacgg gccgggatgg
     2041 gtcatcagga tcaccggctc cggccgggtg aacgtgagct acgcggtgct gcaggcaacc
     2101 ttcaagcccg agtacaggat acacgcgctg cccgactcat cctccctctc catcgactac
     2161 ctcgccacga tcagccaggc caccggcatg gactgggagg tcggcgagct cctcgtggct
     2221 acgggaaggc cctcccacgg catccatgcg cccgagcccg tgccatggca cgtgggaagc
     2281 gcaaagaggg tcttcggcaa ggcctcggcc gaggcgcagg acatctcgcc gttgaccgca
     2341 ccggccccgg ccggggcgct cacgcccgat acggaaccca cgaccgtgac caccgtcatc
     2401 ggcgtcgcga aggacgtcag gctcccgggg gacggcacgc ccgtcaccgc tgcggtcgct
     2461 ggaatccgcc tggatgcggc ctttacggcc atcgtcgtgc ccaggctcga ccagagggca
     2521 tacctgaggg gggagaccac gctcgcgggt gcaatccctg tcgtcgaggg accctacgcc
     2581 gccttcgtgg acggcgtgta tgcgggaagc ggcatgatgg gacggttcgc acccggccag
     2641 aagatgacgg tcgatttcgg cgtgcaggag ggcatcctc
//
LOCUS       DAOSCR010000390         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24770_length_2676_cov_2.951545, whole genome shotgun sequence.
ACCESSION   DAOSCR010000390 DAOSCR010000000
VERSION     DAOSCR010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24770_length_2676_cov_2.951545"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="PLT69_09345"
     CDS             <1..1861
                     /locus_tag="PLT69_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ14682.1"
                     /translation="PQEQERGITITSAVTTCEWKGHEIHIIDTPGHVDFTIEVERSLR
                     VLDGAVVILCGVGGVEAQTETVWQQCRKYNVAALAFVNKLDRPGADFSAAVQQIRQRL
                     EVTPLVIQIPYYEGDVFKGVIDLIGRRLLVWEDTLGASIETLPVPPDAAEETASAREK
                     MVELIAEHDDRVMELYLSGEDPGEETIKEAVRRLTIERLLVPVMCGSALRNKGIQPLI
                     DGIVEYLPSPREAVPAVAHKRDTQELVEIPPDPGGDLVAYVFKVYMDEGRRMVFLRIY
                     SGVLEVGDEVYNSTRRVSEKIARLFSMHAHVKQRIERATAGDIVAAVGLRDSVTGDTL
                     TMPGGDIVLEPIEAQKPVISMAIEPKSSEYTERLQEAMRKIMEEDPTIKIHEDPDTGQ
                     VILAGMGELHLEIAIERLRTAFGVEINVGRPQVLYCSTVEGDSRAEKVFEKTFGDTQH
                     HGHVEVTVRPARRGTGNVFKVGDAVEDGLREYVLMGLEEACLADPEYGHEVVDVEVQV
                     DRVCRTDKTTPQGLKIASQMALHEAMRKAKVVRLEPIMELDALSPEDYVGEVVGDLSA
                     RKASIEGVVTKGRIHEIKAYVPLSNTFGYSTALRSLTRGRGSFSMRFSGFDKV"
     gene            1848..>2676
                     /locus_tag="PLT69_09350"
     CDS             1848..>2676
                     /locus_tag="PLT69_09350"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOJ14683.1"
                     /translation="MTRFSAQTLRHVSLLAVALVIAAFAWVISGILVSVIGHALYEPP
                     SPPKAQPARVVAKKPVRPLSKADYQVVVERDLFKVGRARPVDVNQQVDGDAAHPVAQM
                     GVTLRGTITGPREISRAVIEDAGDQKLYRIGESVKSATIIAILRNKVILDVNGQQQML
                     VVEERASNGKVASARASTRTPRRPRAAPASAGDAMKNLDQLVGNARVVPYYRGGQPYG
                     FRVSDVVEGSEIYNQGMRTGDIIKSVNGVPIRTPQYALNAYQELQNSTTVQLEVERQG
                     "
BASE COUNT          538 a          785 c          964 g          389 t
ORIGIN      
        1 gccccaggag caggaacggg ggatcacgat cacctcggcg gtgaccacct gcgagtggaa
       61 gggtcacgag atccacatca tagacacacc cggccacgtc gatttcacca tcgaggtcga
      121 gcggagcctg agggtcttgg acggggcggt cgtgatcctg tgcggcgtcg gcggcgtcga
      181 ggcccagacc gagacggtct ggcagcaatg ccgcaagtac aacgtggccg cgctcgcctt
      241 cgtgaacaag ctggacaggc cgggcgccga tttctcggct gccgtgcagc agatcaggca
      301 gcgcctcgag gtgaccccgc tcgtcatcca gatcccgtac tacgaggggg acgtgttcaa
      361 gggggtcata gatctcatcg ggcggaggct tcttgtctgg gaagacacgc tcggcgcgag
      421 catagagacc ctgccggtgc cccccgatgc ggccgaggag acagcatcgg cgcgggagaa
      481 gatggtggag ctcatcgccg agcacgacga cagggtcatg gagctctacc tctcgggcga
      541 ggacccgggc gaggagacga tcaaggaggc ggtgaggagg ctgacgatcg agcgtctcct
      601 cgtgcccgtc atgtgcgggt ccgcactcag gaacaagggg atacagcccc tcatcgacgg
      661 catcgtcgag tacctcccct cgccgcggga ggcggtgcct gccgtggcgc acaagaggga
      721 cacgcaggag ctggtggaga tacccccgga ccccgggggg gacctggtcg cctacgtgtt
      781 caaggtatac atggacgagg ggaggaggat ggtcttcctg aggatatact cgggcgtcct
      841 ggaagtgggc gacgaggtct acaactcgac ccgcagggtg agcgagaaga tcgccaggct
      901 cttttccatg cacgcccacg tcaagcagcg catagagagg gccacggcgg gcgacatcgt
      961 cgccgccgtc gggctcaggg actcggtgac cggcgacacc ctcacgatgc ccgggggcga
     1021 catcgtgctc gaacccatag aggcgcagaa gcccgtgatc tcgatggcca tcgaaccgaa
     1081 gagctccgag tacaccgaga ggctgcagga ggccatgagg aagatcatgg aggaggaccc
     1141 gaccatcaag atccacgagg acccggacac cgggcaggtc atactcgccg gcatggggga
     1201 actccacctc gagatagcca tagagaggct ccgcacggcc ttcggcgtcg agataaacgt
     1261 gggcaggccg caggtgctct actgctcgac cgtggaaggc gactcgcggg ccgagaaggt
     1321 cttcgagaag accttcgggg acacgcagca ccacgggcac gtggaggtca cggtcaggcc
     1381 ggccaggcgc gggaccggca acgtgttcaa ggtcggggac gcggtggagg acgggctcag
     1441 ggagtacgtg ctgatggggc tcgaagaggc gtgcctggcc gaccccgagt acgggcacga
     1501 ggtcgtggac gtggaggtgc aggtcgacag ggtgtgcagg acggacaaga ccacgcccca
     1561 gggattgaag atcgcctccc agatggcgct gcacgaggcc atgaggaagg cgaaggtggt
     1621 gaggctcgag cccatcatgg agcttgacgc gctgtccccc gaagattatg tgggtgaagt
     1681 ggtgggcgat ctttcggcga ggaaggcgag catcgaggga gtcgtcacca aggggaggat
     1741 acacgagatc aaggcgtatg tgcctctctc gaacaccttc ggctactcga ccgccctgag
     1801 atccctcacg aggggcaggg ggagcttcag catgaggttt tccgggtttg acaaggttta
     1861 gcgcacagac tctcaggcac gtgagtctcc tggccgtcgc cctggtgatc gccgcattcg
     1921 cctgggtcat atcgggtatc ctcgtgtccg tgatcgggca cgccctctac gagccgccct
     1981 cgccgcccaa ggcccaaccc gcccgggtgg tcgcgaagaa gcccgtcagg cccctctcga
     2041 aggcggacta ccaggtggtg gtcgagaggg atctcttcaa ggtcgggcgc gcgaggcccg
     2101 tcgatgtgaa ccagcaggtg gacggggacg cggcgcaccc ggtggcccag atgggcgtca
     2161 cgctcagggg gaccatcacg gggccgcgcg agatatcgag ggccgtcata gaggatgccg
     2221 gcgaccagaa gctctaccgg ataggggagt cggtcaagag cgccacgatc atcgcgatcc
     2281 tcaggaacaa ggtgatactc gacgtgaacg gccagcagca gatgctcgtc gtggaggagc
     2341 gcgcatccaa cggcaaggtc gcatcggcga gggcttccac gcggacgccc aggaggccgc
     2401 gtgcggcgcc cgcctcggct ggggacgcca tgaagaacct ggaccagctc gtggggaacg
     2461 ccagggtggt cccgtactac aggggcgggc agccctacgg gttcagggtg agtgacgtgg
     2521 tcgagggctc cgagatctac aaccagggga tgcgcacggg ggacatcatc aagtccgtca
     2581 acggggtccc catacggact ccccagtacg cgttgaacgc ataccaggag ctccagaact
     2641 ccaccacggt gcagctggag gtggagaggc aggggg
//
LOCUS       DAOSCR010000391         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24773_length_2676_cov_2.742846, whole genome shotgun sequence.
ACCESSION   DAOSCR010000391 DAOSCR010000000
VERSION     DAOSCR010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24773_length_2676_cov_2.742846"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLT69_09355"
     CDS             <1..208
                     /locus_tag="PLT69_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ14684.1"
                     /translation="NVGADAYNQTLSERRASSVGQYLISQGIDPRRIYTQGFGETKPI
                     ASNDTEAGRQQNRRVEIQLSPLTQ"
     gene            complement(751..1224)
                     /gene="moaC"
                     /locus_tag="PLT69_09360"
     CDS             complement(751..1224)
                     /gene="moaC"
                     /locus_tag="PLT69_09360"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOJ14685.1"
                     /translation="MALTHTDEKGRASMVDVSDKPRTRRVARASGRITMNEEAYRAVV
                     TNTAKKGDVLAAARIAGIMAAKNTHAVIPLCHPLPIDHVSLELTPEDGTRSIRAEATV
                     TTTGRTGVELEALHAVSVCLLTVYDMVKAVDRSMTIGDIVLEEKSGGKSGHYTRT"
     gene            complement(1233..1517)
                     /locus_tag="PLT69_09365"
     CDS             complement(1233..1517)
                     /locus_tag="PLT69_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ14686.1"
                     /translation="MPIYEYRCTKCLREFESMQKFSDEPIRTCPDCGGKAKRLISRSS
                     FQLKGSGWYLTDYARKSSSGDSGGDSTSSDSSGKKESGTSSTSSSGSSDE"
     gene            complement(1504..2028)
                     /locus_tag="PLT69_09370"
     CDS             complement(1504..2028)
                     /locus_tag="PLT69_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14687.1"
                     /translation="MGYLLPEHSSALRYGSLLAEQVLSRSLGWHAGDLYDVTTVSEID
                     SSARPANRFAQLLRVEVALPHMPHHYRVCLLDDEILSFLEEHLDIDLVSLITFIMTHE
                     LLHVHRFSTGKADFFGDPVSEEVVVDALTRVILAKYPVIGLKRVLSLLDRLKAAPLYN
                     GHIMNEPRCVDAYL"
     gene            complement(2076..>2676)
                     /gene="aroF"
                     /locus_tag="PLT69_09375"
     CDS             complement(2076..>2676)
                     /gene="aroF"
                     /locus_tag="PLT69_09375"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ14688.1"
                     /translation="FQGLKNDGLLLLSMARDEFKLPVVTEVMDPAHIEVVLENADCLQ
                     IGARNIQNFQLLREVGRTRKPVLLKRGMSTTIEELLMSAEYVLSGGNHDVILCERGIR
                     TFETATRNTLDLSAVPVLKEKTHLPVIVDPSHGTGHARYVEAMAYAAVAAGADGIMIE
                     VHPRPEEALSDGPQSLRPEEFRRIMEGVRNVARAVGRDV"
BASE COUNT          456 a          847 c          884 g          489 t
ORIGIN      
        1 caacgtgggt gccgacgcct acaaccagac gctctcggag agacgggcct ccagcgtggg
       61 ccagtatctc atatcgcagg gcatcgaccc caggaggatc tacacccagg ggttcggcga
      121 gaccaagccc attgcgagca acgataccga ggcgggccgc cagcagaaca ggagggtcga
      181 gatccagctc agcccgctga cccagtgagc ggtgcacctc caggcggccg tgacgtcaag
      241 ggaccgcggc cgcctctgca atgtgaaccg agggggatgg gcagggtgag acacgcagcc
      301 tgcccgtccc ttcctgtttc ccgcgaggcc cgaaaccgtc atccacaggc gtacggcatg
      361 gcggggtttg tgtgtccgcg gctccaggaa caggcagggc gaggatcggt cgcccgggtc
      421 tgcggctgtc gaggccggtg gcaccagacg ccttgagacc cgctcttggc tgagcccttg
      481 acaaccagtc cgtggtcgat tcccggggac gggttgtcca tggagggggc atggagcccg
      541 cggcccgatg cggtacggcc ccctgacgtc ttgaccctct cggtgtgtcc tgagaccctt
      601 tcctcgaggg gaaccacggt ggacgtttcg tgaccctgcc caaggacgcc tcgcgccttg
      661 aggcgaaggg cggcgggcat gccatgcgtt atgacgggcc ccgtgatgat gcagccctgc
      721 gcttggcgcc aggtacctga agagcggggg tcacgtcctc gtgtaatgcc cgctctttcc
      781 cccggacttt tcctcgagga cgatgtcgcc gatggtcatg gacctgtcca cggccttgac
      841 catgtcgtag acggtgagca ggcagacgct caccgcgtgg agcgcctcca actcgacccc
      901 tgtcctgcct gtcgtcgtga ccgtggcctc ggcccttatg gacctcgtcc catcctcggg
      961 cgtgagctcc agggacacgt ggtcgatggg cagggggtgg cacaggggga tcaccgcgtg
     1021 ggtgttcttt gcggccatga tcccggcgat cctagccgcc gccagcacat cacccttctt
     1081 cgcggtgttg gtcacgaccg cacggtatgc ctcttcgttc atggtgatgc ggccggacgc
     1141 tctggccacg cgcctcgtgc gaggcttgtc cgacacgtcc accatggagg cacggccctt
     1201 ctcgtctgtg tgcgtaaggg ccatgccagg gctcactcgt cgctcgaacc cgatgagctc
     1261 gtggaagacg tgcccgattc cttcttgccg gacgagtccg aagaggtgga atcgccgccg
     1321 gagtcgcccg acgaggactt cctggcgtag tcggtcaggt accaacccga tcccttgagc
     1381 tggaaggacg aacgtgagat gagtctcttc gccttcccac cgcagtccgg gcaggtacgg
     1441 atgggctcgt cgctgaactt ctgcatggac tcgaactcgc gcaggcactt ggtgcaacgg
     1501 tattcataga taggcatcga cacacctcgg ttcgttcatg atgtgaccgt tatatagtgg
     1561 cgccgctttc aacctgtcaa gaagggacag cacccttttc aggccgatga cgggatactt
     1621 cgcgaggatc accctcgtga gggcgtccac gaccacctcc tcgcttacgg ggtcgccgaa
     1681 gaagtcggcc ttgccggtgg aaaaccggtg gacgtgcaaa agctcgtggg tcatgatgaa
     1741 ggtgatgagg gagacgagat cgatgtccag gtgttcctcg aggaacgaga ggatctcgtc
     1801 gtcgagcagg cacacccggt agtggtgtgg catgtgcgga agggccacct ccaccctgag
     1861 gagctgggcg aacctgttgg ccggtctcgc ggacgagtcg atctcggaca cggttgtcac
     1921 gtcgtagagg tctcccgcat gccaccccag ggagcgggac aggacctgct cggccaggag
     1981 cgacccgtac ctcagtgcag agctgtgctc gggcagcaag taacccattt caccttccat
     2041 tataggaaca catgcagagc gggtcaaccg ccgccctaca cgtccctgcc caccgcccgg
     2101 gcgacgttcc tgacgccctc catgatgcgc ctgaactcct cgggtctcaa ggactgcggc
     2161 ccgtccgaga gggcctcctc gggcctgggg tggacctcga tcatgatgcc gtccgcccct
     2221 gcggccaccg ccgcgtacgc catcgcctcc acgtacctgg catggccggt gccgtgggac
     2281 gggtccacga tgaccgggag gtgcgtcttc tccttgagca ccgggacggc ggagaggtcg
     2341 agggtgttgc gcgtggccgt ctcgaaggtc ctgatgcccc gctcgcagag gatgacatcg
     2401 tggttgccgc cggagagcac gtactcggcg gacatgagca actcctcgat ggtggtggac
     2461 atgccccgct tgaggagcac gggcttgcgg gttctcccca cctccctgag gagctggaag
     2521 ttctggatgt tgcgggcacc gatctggagg cagtcagcat tctccaggac cacctcgatg
     2581 tgggccgggt ccatcacctc ggtcaccacg gggagcttga actcgtcgcg ggccatggag
     2641 aggaggagca ggccgtcgtt cttcaggccc tggaag
//
LOCUS       DAOSCR010000392         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24967_length_2662_cov_3.580744, whole genome shotgun sequence.
ACCESSION   DAOSCR010000392 DAOSCR010000000
VERSION     DAOSCR010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24967_length_2662_cov_3.580744"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLT69_09380"
     CDS             complement(<1..269)
                     /locus_tag="PLT69_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0F1 subunit beta"
                     /protein_id="HOJ14689.1"
                     /translation="MSEQVNRGTVVSVRGSVVDAWFPQRLPDIFNVLRTGDDNRIVIE
                     VNTHLNANTVRGIALTPTQGLGRGAEVVDTGRFLEVPVGKGLLGRV"
     gene            complement(504..1301)
                     /locus_tag="PLT69_09385"
     CDS             complement(504..1301)
                     /locus_tag="PLT69_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOJ14690.1"
                     /translation="MAGHAGTRSLLLCATVAALALIVAGCALRKVEVAPLKTRGVPKP
                     RAMITAQKMGYAIQVGAFRDVGNAARLTRKLESRGIDAFYFHHASGFFKVRFGDYPTL
                     STAAEEARRLKDAGIIDVYFVVNPGEYAVARGKSGAGPSLRADIVETAMEFVGLPYLW
                     GGADLDAPLDCSGLVMAVYRLNGLDVPRTSQEQYEGGTPVDMEELEAGDLVFFSTSGN
                     GRVSHVGIYIGDGRFIHAPRRGKTICADSLSSGYYRDRFQGARSYLR"
     gene            1707..2219
                     /locus_tag="PLT69_09390"
     CDS             1707..2219
                     /locus_tag="PLT69_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOJ14691.1"
                     /translation="MKAALDVWYDKDTFCACCVVFEHWEDARASEVVTLSGTGVGPYR
                     PGRFFERELPCLLAVLERASIPFDAVVIDGYVDLDGSRGKGLGRHLYEALGGRSIVVG
                     VAKSPLSFACRFVPIFRGSSKRPVYVSGAGCPEDLAAAWVSRMHGPYRIPTLLALAHR
                     SAVSCGRRRP"
BASE COUNT          474 a          911 c          796 g          481 t
ORIGIN      
        1 accctgccga ggagcccctt gcccacaggg acctccagga acctccccgt gtcgacgacc
       61 tccgcccccc tgccgagccc ctgggtgggg gtcagggcga tgccgcgtac cgtgttggcg
      121 ttcaggtggg tgttgacctc gatgacgatc cggttatcgt cgcctgtcct gaggacgttg
      181 aagatgtcgg ggagacgctg ggggaaccaa gcgtccacga cactccccct cacggatacc
      241 accgttcccc ggttgacctg ctcactcata cgggcgggac ctccttcagg tatcagacta
      301 tgatactctg gctgccctag acatccccgg tgcagtgcac aaccgaatga gcatcttatt
      361 cattacacat tacgactatc tccagagctg tcaagaggga aacaggcccc caccccaaaa
      421 tttcctcaaa gaatccgagg aactagcctc tcacggcccc tcgatgtatc ggccaaacag
      481 gatgtgtttt ccgatgccgg atttcatctc aagtagcttc gtgcgccttg gaagcggtct
      541 cggtaatatc cgctggagag agaatcggca caaatggttt ttcctcttct cggcgcatgg
      601 atgaacctgc cgtcccctat gtagataccc acgtggctca ccctgccgtt tcccgaggtg
      661 gagaagaaca cgagatcgcc tgcctcgagc tcttccatgt ccaccggggt ccctccctcg
      721 tactgctcct gtgatgtccg ggggacgtcc aggccgttga gcctgtagac cgccatgacg
      781 agcccgctgc agtcgagagg cgcatccagg tccgcgcccc cccagaggta gggtagaccg
      841 acgaactcca tcgccgtctc cacgatatcc gcccgcaagg acgggcctgc cccgctcttg
      901 ccccgggcga cggcgtactc gccgggattg accacgaagt agacgtctat gataccggcg
      961 tccttgagtc gcctcgcctc ttcagccgct gtggaaagcg tcgggtagtc cccgaaccga
     1021 accttgaaga accccgaggc gtggtggaag tagaaggcgt ctatgcccct ggattcgagc
     1081 ttgcgggtga gcctggcggc gttccccacg tccctgaatg cgcccacctg gatggcatac
     1141 cccatctttt gggccgtgat catcgcgcgc ggcttgggga cgcccctggt cttcaagggt
     1201 gcgacctcca ccttgcgcag ggcgcacccg gcaacgatca acgccagggc ggcgacggtc
     1261 gcgcacagga ggagacttcg cgtccccgca tgccctgcca tccttccccc tcgtgtccct
     1321 gtcgttcaag ggcgatgttc gtaacgtagc ccatccctga caacgccttt ctcataacaa
     1381 cacgatcggc cggtgtccac cgtatccttg aggaaccgct cacccaggca tcggccccgt
     1441 caccgggaga tccctccttg ccgtcaacca cgtaccgacg gttccgcacg cctgccgggg
     1501 acggccctga gcagggcagc ttacggcctc tcttgatctg gccttcagaa cgatgcatac
     1561 aggaggacgg gtcgacgctc cacctgctag gggacgaacc atgagcccgg aaacgccgga
     1621 tcgcctgcgt cccctgggcc catggagcct ctcgggacga cccgtgcgca aggcagagga
     1681 acaagagggg aggtggcgac accctcatga aggccgccct cgatgtgtgg tacgacaagg
     1741 acaccttctg cgcctgctgt gtcgtcttcg aacattggga agacgcccgg gcctccgagg
     1801 tggtgaccct ttccgggacg ggtgtcgggc cgtacaggcc agggagattc ttcgagcggg
     1861 aactgccgtg cctcttggcg gtgctggaaa gagcatcgat accgttcgac gccgtcgtca
     1921 tcgacggcta cgtggacctg gacggttcac ggggaaaggg gctcggcagg catctctacg
     1981 aggccctcgg gggcaggtcg atcgtcgttg gcgttgcgaa gagcccgctc tctttcgcct
     2041 gcaggttcgt acccatcttc cgcggctcga gcaagaggcc ggtctacgtc tcgggggccg
     2101 gctgcccgga agacctcgcc gcggcctggg tctcgcgcat gcacggaccg tacaggatcc
     2161 ccaccctgct tgcactggcc cacagatccg ccgtgtcctg cgggaggcgc cggccatgac
     2221 ccgcatcgaa ccccggtgcc ctgatacccg ggttgcaaga tcagcctggc acccgggata
     2281 ccctggacgc cttgcgccgg tgaaacctgc aggcaggctc cgtgacccgt tgacgccccc
     2341 tcgaccccgg tgcccgatct ccacacatag gctcatgctc cataccgggg ggtggttcga
     2401 tggcatcccc cgccctgccc gccggcccca ctcggggggc atccccgatg catgccgggt
     2461 cggaaaggca tccctggccg acgatcgcgg gctccagacc tgacgcccag ggatgccatc
     2521 tcgcacccca gggcctgagg atgcataccc gcgtgggccc aaaaaacggc aaggcacgaa
     2581 gacggccatg caccgtgggc cgccgctgca ccgctacgaa aaccggctat ggggttccca
     2641 ggtaatccct ttgagctcac cg
//
LOCUS       DAOSCR010000393         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_24973_length_2661_cov_3.793170, whole genome shotgun sequence.
ACCESSION   DAOSCR010000393 DAOSCR010000000
VERSION     DAOSCR010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24973_length_2661_cov_3.793170"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLT69_09395"
     CDS             complement(<1..616)
                     /locus_tag="PLT69_09395"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOJ14692.1"
                     /translation="MDKTAGTLTFYRGNRLETLAERLCDLLSQPCGGPFEPETVVVSS
                     RGMQRWLSLEIAARLKVCANVNFPFQKAFVLDMFSRVLGGKKPGALFAREVMTQRIME
                     VLPCLATGDAWRALEGYLALDPEGLKLYQLSSSLANLFDQYLVYRPDMVAGWEEGKTS
                     GEGDVERWQKELWARAFGAAGFDHPARMADEFCRAVGQAPAEVLP"
     gene            complement(1061..2224)
                     /locus_tag="PLT69_09400"
     CDS             complement(1061..2224)
                     /locus_tag="PLT69_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ14693.1"
                     /translation="MALPLAYNLRNLWTRKVATVLTASGMALVVFVFASSSMLSEGLK
                     KTLVETGSPDNVVVVRKGATSEVDSSVERWQAHVVEALGQAAPSDDGSPLSAREVLVL
                     VTLPRVSGGVSNVAVRGIGRHSAGLRPQVRIVQGRMPRFGAREVAVGSSIARRFAGIV
                     PGGTVRLALDEYRIVGIMDASGTAFSSEVWADADQIMQTFRRPVYSSLVFRLSRPERF
                     KEVQAAIEGDPRLTLKAEPESSYWRKQSELMAMFLEILGMSLALLFSIGAVIGGMVTM
                     HAAVAGRTSEIGTLRALGFGRRHILLGFIAEALILGLAGGLAGLALASLLQFVTVSTV
                     NFQTFSELAFSFELGARTVAESLLFSLAMGFAGGFMPALKASRMNIVEALRTA"
     gene            complement(2225..>2661)
                     /locus_tag="PLT69_09405"
     CDS             complement(2225..>2661)
                     /locus_tag="PLT69_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ14694.1"
                     /translation="RAFVLSFISMSEAIVKIVKVVSLVVIAIIMVVVANTMAMTTRER
                     TGEYAVLKTLGFSWLSIVMVIAGESWFISAAGCLTGVIAAFPAVGFLTTKLSAYFPVF
                     FIDAETVALDVVVAFLVGTVSMVIPSWQTCCIRVADALKKVG"
BASE COUNT          483 a          932 c          846 g          400 t
ORIGIN      
        1 tgggaagcac ctccgccggg gcctggccca cggcgcggca gaactcgtct gccatgcggg
       61 ccggatggtc gaaccccgcg gcgccgaagg ctctcgccca gagctccttc tgccaccgct
      121 cgacatcgcc ttccccggag gtctttccct cctcccaccc ggccaccata tccggcctgt
      181 agacgaggta ctggtcgaag aggttcgcga gtgaggacga aagctggtag agcttcaggc
      241 cctcggggtc cagggcgagg tacccttcga gggcccgcca ggcgtccccg gtcgccaggc
      301 aggggagcac ctccatgatg cgctgcgtca tgacctcgcg cgcaaacagg gcgccgggct
      361 tcttgccgcc cagcaccctc gagaacatgt cgagcacgaa ggccttctgg aaagggaagt
      421 tcacgttggc gcagaccttg agcctggcgg ctatctcgag ggagagccac ctctgcatgc
      481 ccctgctgct caccacgacg gtctccggtt cgaagggccc gccgcagggc tgcgacagga
      541 ggtcacagag cctttccgcc agtgtctcga gacggttgcc ccggtagaac gtcaacgttc
      601 ccgctgtttt atccacgccc aggcccattg cactctcttc cgagtatacc acggtcacgg
      661 atggagggca tcgggcattt gggcatgatc ggaggccctc cttcggggcc ttcggccgca
      721 gccggtgagc gacggaggct ctcgcgaaga cagcagcttt acgggccgcg gccgtgaagc
      781 acccctgcag ggccgcacgc gccctactgg acgcggccaa gacccgcaca ccccgtcaca
      841 cgagcgcctt cgctgccgca cgggcagcgc ccgatcgcac cccttcgcgc gtctgcttgc
      901 gggcgcgcga aggaggcgaa ggccgacgcc cctgggctcc gagatgcgga ttcgcttccg
      961 acgcgctcga actccctgtc ttcccgacgc ccctgaacgc caagatgcag cacatcaccg
     1021 actttcaagc caagccggcg aaagacgacc gtctgccggc ctaagccgtg cggagcgcct
     1081 ccacgatgtt catgcgcgat gccttcaagg ccggcatgaa cccgcctgca aaccccatcg
     1141 ccagggagaa cagcagggac tcggccacgg tgcgggcccc gagctcgaag gagaaggcga
     1201 gttccgagaa ggtctggaag ttgacggtcg agacggtcac gaactgcagg agcgaggcca
     1261 gggcgagccc cgccaggccg ccagcgaggc cgaggatgag cgcctcggcg atgaacccga
     1321 ggagaatgtg cctgcggccg aacccgagcg ccctgagggt gcctatctcg ctcgtcctgc
     1381 ccgcgaccgc cgcgtgcatg gtgaccatgc cgccgatgac cgccccgatc gagaagagga
     1441 gggcgagcga catgccgagg atctcgagga acatcgccat gagctccgat tgcttgcgcc
     1501 agtaggacga ctcgggctcg gccttgaggg tgagcctcgg gtcgccctcg atggcggcct
     1561 gtacctcctt gaacctctcg ggcctcgaca gcctgaacac gagggaggag tacacggggc
     1621 ggcggaaggt ctgcatgatc tggtccgcat cggcccagac ctccgatgag aacgccgttc
     1681 cggatgcgtc catgatcccc acgatccggt actcgtccag ggcgagcctc accgtgccgc
     1741 ccgggacgat gcccgcgaac cgtctcgcga tgctggagcc cacggcgacc tctctcgccc
     1801 cgaagcgggg catacggccc tggacgatcc tcacctgggg cctgagcccg gccgagtgtc
     1861 tcccgatccc cctgaccgcg acgttcgata ccccaccgga caccctggga agcgtcacca
     1921 ggacgagcac ctccctggcg gacaggggcg agccgtcgtc ggagggggcc gcttggccga
     1981 gcgcttccac gacgtgggcc tgccacctct ccacggagct gtccacctcc gaggtcgccc
     2041 ccttcctgac caccaccacg ttgtcgggtg agcccgtctc caccagggtc ttcttgaggc
     2101 cttccgacag catggacgac gacgcgaaaa cgaagaccac gagcgccatg cccgaggcgg
     2161 tgagcaccgt ggccaccttg cgtgtccaca ggttgcgcag gttgtaggca aggggaagcg
     2221 ccatctatcc caccttcttg agggcgtccg ccaccctgat gcagcacgtc tgccacgagg
     2281 ggatgaccat ggagaccgtg cccacgagga acgcgaccac cacgtcgagc gccacggtct
     2341 cggcgtctat gaagaacacg gggaagtacg cgctcagctt cgtggtgagg aagcccacgg
     2401 ccgggaaggc ggcgatcacg ccggtgaggc accctgcagc tgagatgaac caggactccc
     2461 ccgcgatcac catcacgatg ctcagccacg agaagccgag cgtcttgagc accgcgtact
     2521 cgcccgtgcg ttcgcgtgtc gtcatggcca tggtgttcgc gaccaccacc atgatgatcg
     2581 cgatcaccac gagagagacc accttcacga tcttcacgat ggcctcgctc atggagatga
     2641 agctcaagac gaacgcgcgc t
//
LOCUS       DAOSCR010000394         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25097_length_2652_cov_3.169811, whole genome shotgun sequence.
ACCESSION   DAOSCR010000394 DAOSCR010000000
VERSION     DAOSCR010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25097_length_2652_cov_3.169811"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..768)
                     /locus_tag="PLT69_09410"
     CDS             complement(70..768)
                     /locus_tag="PLT69_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ14695.1"
                     /translation="MDLHDVKWALAVGMEGIGGYAEDEFASFVRRGSDRLLPVAFLNP
                     SRFGPRRDLSRHVSSLRDKGYVGIKIHPRLSHTPLTHPNLPAAVNEAAEQGLVTLICT
                     YPYAPGMDRDTYALDALVSLLDKTKGCKVILLHAGCVRLLEMIEIARLFDDVLLDLSF
                     TICKYAGSSIDLDIAYAFRQFDRRVCIGSDFPEFDLKLLRERFSAFADGLPGEKIENI
                     AYKNLLNFMGDIVL"
     gene            complement(933..1589)
                     /locus_tag="PLT69_09415"
     CDS             complement(933..1589)
                     /locus_tag="PLT69_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOJ14696.1"
                     /translation="MDSHEWKEKIIDYIKRNRVSSTEVADCLGKTGAVPDVRPISEGH
                     FRVGNVFWAYAYGESNWDVHERLQHVQEGDVALVEVFDCKDRAVFGDLVSKFLILYRQ
                     VSAIVVRGYLRDAHRLIKERWPIWCTGFNPVGCFNQKVDTPFDRDTFEDRLRRYHGAI
                     GVCDDTGVVIIPKEHHTEEFFHKLEFIEQQEDIWYECIDRRKWTTFETVCLKKYADKA
                     "
     gene            complement(1626..>2652)
                     /locus_tag="PLT69_09420"
     CDS             complement(1626..>2652)
                     /locus_tag="PLT69_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ14697.1"
                     /translation="RNLEIARKYRPDAIVTDQTDIAVPTVAYLCEKMRLPGIGMRAAT
                     LFTDKYAMRTFLKDHGFSTPAFMLCKTMEDALEFADEHGYPLVIKPPASQSSRGVHKV
                     TSEEDLRASYTDTLRFSPAGCILAEEYIEGVEFTVEGFKTAEKAYSLVVSKKRPFRHS
                     EMIASRLLYRHWDPDFDYPQLMELNERIVTCMGLPFGITHAEYKCRDGTFYLIEIAAR
                     GGGTKISSHIVPEMTGLDINELLIRCALGEHITEIHFERRDHCVVLEFLLFDPGKVAS
                     ISGIDWLSSNDSIIDFGLNFGVGDVLAPPSDDRSRHAYLIARAETPGDIERILNEART
                     RVTITYA"
BASE COUNT          493 a          784 c          761 g          614 t
ORIGIN      
        1 agtacccgta tctgccgagc cgttccctgt acctggtgat gacgaggtcg acatcatggc
       61 cggaaagttt catagtacga tgtctcccat gaagttcagc aagttcttgt aggcgatgtt
      121 ctcgatcttc tctccgggaa ggccgtcggc aaatgcactg aaccgctctc tcaaaagctt
      181 gagatcgaac tccgggaagt cggaacctat gcataccctc ctgtcaaatt gcctgaacgc
      241 ataggctatg tccaggtcga tggagcttcc cgcgtacttg cagatggtga aggaaagatc
      301 caggagaacg tcgtcgaaga gcctcgcgat ctcgatcatc tcgaggagac gcacgcaacc
      361 ggcatgaagg aggatcacct tgcacccttt cgtcttgtcg aggagggaca cgagcgcgtc
      421 cagggcgtat gtgtccctgt ccatcccggg ggcatacgga tacgtacaaa tgagggtcac
      481 caggccctgt tctgccgcct cgttcacggc cgccgggagg ttcgggtggg taaggggggt
      541 gtgggaaagc ctcgggtgga tctttatccc gacgtacccc ttgtctctca aggaggagac
      601 atggcgggaa agatccctgc gtgggccgaa ccgtgaaggg ttcaagaacg ccactggcaa
      661 gagcctgtcc gagccccttc tcacgaaact ggcgaactcg tcttcggcat agccgccgat
      721 cccttccatg ccaacggcca gggcccactt cacgtcatga agatccatct gctccttcag
      781 gctgtccatg tgggccgatg tctgagggcg cccgggggtc caccccccgg tgatggtggg
      841 atgtgtcagc gcgtcgaaga tgggaacaac gggggtgggt tccccgcgcc ctgtctcatc
      901 gctgtgcatt tcctgccctg ccgcctcctg tttcaggcct tgtctgcata cttcttgagg
      961 cacaccgtct cgaaggtcgt ccattttctc ctgtcgatgc actcgtacca gatgtcttcc
     1021 tgctgttcta tgaactcgag cttatggaag aactcctcgg tatgatgctc cttggggatg
     1081 atgaccacgc ccgtatcgtc gcacacgccg atcgccccgt ggtaccgtct cagacggtct
     1141 tcgaaggtgt ctcggtcgaa gggggtgtcg accttctggt tgaagcaccc gacagggttg
     1201 aaccccgtgc accatatcgg ccatcgttct ttgatgagac ggtgtgcgtc cctgagatac
     1261 cccctcacca cgatcgccga gacctgcctg tacaggatga ggaacttgga cacgaggtca
     1321 ccgaaaacgg ccctgtcctt gcagtcgaac acctctacga gggccacgtc gccctcctgt
     1381 acgtgctgga gccgttcgtg cacatcccag ttgctctctc cgtaggcata cgcccagaag
     1441 acgttcccga ccctgaaatg tccttcgctt atgggcctga catccggcac ggcgccggtc
     1501 ttaccgaggc agtccgccac ctcggtggag gagaccctgt ttctcttgat gtaatcgatg
     1561 atcttttcct tccactcgtg ggaatccacc gttgtacccc cttcgcttat gtgttgagat
     1621 tccttttatg cgtacgtgat ggtcaccctt gtccttgcct cgttcaggat cctttctatg
     1681 tcccccggcg tttcagccct cgcgatgagg taggcgtgcc gagacctgtc gtctgacggc
     1741 ggggcaagca cgtctcccac accgaagttc aggccgaagt cgatgatcga gtcgttcgaa
     1801 gagagccaat cgatgccgga gattgacgcg acctttccgg ggtcgaacag aaggaactcg
     1861 aggacgacac agtggtccct gcgttcgaag tggatctcgg ttatgtgctc tcccaaggcg
     1921 cacctgatga ggagctcgtt tatatcgaga cccgtcatct cggggacgat gtgagacgat
     1981 atcttcgtcc ctcccccgcg ggccgctatc tcgatgaggt agaacgtccc gtctcgacac
     2041 ttgtactcgg catgtgtgat gccgaagggc aggcccatac aggtgacgat ccgctcgttg
     2101 agttccatga gctggggata atcgaagtcc gggtcccagt ggcggtagag aagccgcgat
     2161 gctatcatct ccgagtgcct gaacggcctc tttttcgata ccacgagcga gtaggccttt
     2221 tccgctgtct tgaacccctc gaccgtgaac tctacgcctt cgatgtactc ctccgcgagg
     2281 atgcatcccg cagggctgaa cctcagggtg tccgtgtagc ttgcacggag gtcttcctca
     2341 gacgtcacct tgtgcacccc cctgctcgat tggctggccg gtggctttat gaccaagggg
     2401 tatccgtgtt catcagcgaa ctccaatgcg tcttccattg ttttgcagag catgaatgcg
     2461 ggcgtggaga acccgtgatc tttcaggaac gtcctcatgg cgtacttgtc ggtgaagagg
     2521 gtcgcggccc tcatgccgat cccgggcagg cgcatctttt cgcaaaggta ggcaacggtc
     2581 gggactgcga tgtctgtctg gtccgtgacg atggcatcag gccggtactt cctcgcgatc
     2641 tcgaggtttc gt
//
LOCUS       DAOSCR010000395         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25266_length_2640_cov_2.476983, whole genome shotgun sequence.
ACCESSION   DAOSCR010000395 DAOSCR010000000
VERSION     DAOSCR010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25266_length_2640_cov_2.476983"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLT69_09425"
     CDS             <1..278
                     /locus_tag="PLT69_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOJ14698.1"
                     /translation="FLFCLLENCILDGCDATDANFGMSYLVNTSMNKANLSGACLACS
                     MMDGVRIRESVIERVDLTLLAARGLDITGSSQKGVIVERAKVQGLIK"
     gene            complement(271..714)
                     /gene="nusB"
                     /locus_tag="PLT69_09430"
     CDS             complement(271..714)
                     /gene="nusB"
                     /locus_tag="PLT69_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ14699.1"
                     /translation="MKQRSRSREVALQYLYQVDMTGSHTEQAMQDFFAHFVEDVELVP
                     YAKDIIAGVCDHLVEIDELIEKASTNWSVSRMSATDRNILRIAAYELAFRDDVPYKVV
                     INEAIEIAKRYGSPKLPSFANGVLDRVWNLISGGRDDGGDQEGLT"
     gene            complement(728..1192)
                     /gene="ribE"
                     /locus_tag="PLT69_09435"
     CDS             complement(728..1192)
                     /gene="ribE"
                     /locus_tag="PLT69_09435"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOJ14700.1"
                     /translation="MAQVIEGKLAGKGKKVAIVASRFNDFVTARLVEGALDALGRHDV
                     AAEDITIYKVPGAFEIPAVAKKVALFAAVDGVICLGAVIRGSTPHFDYVAAEVSKGVA
                     LVGLEAKIPVVFGVLTTDTVEQAIERAGTKAGNKGFDAAMALLEMMDLYGRI"
     gene            complement(1192..2394)
                     /locus_tag="PLT69_09440"
     CDS             complement(1192..2394)
                     /locus_tag="PLT69_09440"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOJ14701.1"
                     /translation="MPTCTVEEAVYELQQGRMIILVDDEDRENEGDLTMAAEFVTAEA
                     INFMAKHGRGLICLALAPEIADRLDLQPMVRDNTSRFGTGVTVSIEAKRGVTTGISAA
                     DRAHTIRTAIDPATRPDDLARPGHVFPLRARPGGTLVRTGQTEGSVDLCRIAGLKPGA
                     VICEIMNDDGTMARRPQLEEFARAFGLKICTIADIIAHRIRTETLVREVVRAKLPAED
                     GSFELVGFDNDIDDKEHIALVKGPIDPEEPVLVRVHSECLTGDVFHSLRCDCGAQLHR
                     AMEMIEEAGRGVIVYMRQEGRGIGLINKLKAYHLQDKGKDTVEANVELGFPADLRDYG
                     IGAQILLHLGVRKMRLMTNNPKKIIGLEGYGLEIVERVPIEIPPTLENQRYLKTKKEK
                     MGHILEVV"
     gene            complement(2400..2612)
                     /locus_tag="PLT69_09445"
     CDS             complement(2400..2612)
                     /locus_tag="PLT69_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14702.1"
                     /translation="MTVNEALGDAFTVNIIPHTAGSTSLTGKGAGVRVNIEFDILGKY
                     VENLLKKRGVGGIETLLEQAGFKRRE"
BASE COUNT          407 a          890 c          809 g          534 t
ORIGIN      
        1 gcttcctctt ctgcctgctc gagaactgca tcctcgacgg gtgcgacgcg acggacgcga
       61 acttcgggat gtcctacctg gtgaacacga gcatgaacaa ggcgaacctg agcggcgcct
      121 gtctcgcctg cagcatgatg gacggcgtca ggatcaggga gtcggtcatc gagcgcgtcg
      181 acctgacgct ccttgcggca cggggtctcg acatcaccgg gagctcgcag aagggcgtca
      241 tcgtggagcg ggccaaggtg cagggcctca tcaagtgagg ccttcctgat ccccgccgtc
      301 gtcccgaccg cccgaaatca ggttccagac cctgtcgagc accccgttgg cgaacgacgg
      361 cagcttcggt gacccgtacc tcttggctat ctcgatggcc tcgttgatga ccaccttgta
      421 gggcacgtcg tcgcggaagg ccagctcgta ggcggcgatg cgcaggatgt tgcggtcggt
      481 cgcggacatg cggctgacgc tccagttcgt ggacgccttc tctatgagct cgtcaatctc
      541 cacgaggtgg tcgcacacgc ccgcaatgat gtccttggcg tacggaacga gctccacgtc
      601 ctcgacgaag tgggcgaaga aatcttgcat ggcctgctcg gtgtggctcc ccgtcatgtc
      661 cacttggtag aggtactgga gggccacttc cctcgaccta ctcctctgtt tcatggacct
      721 gcgccggtca gatgcggccg tagagatcca tcatctcgag gagcgccatg gcggcgtcga
      781 agcccttgtt ccccgccttg gtgcctgctc gctcgatggc ctgctcgacg gtgtcggtgg
      841 tgaggacccc gaagaccacg gggatcttgg cctcgaggcc cacgagcgcc acgcccttgc
      901 tcacctcggc cgccacgtaa tcgaagtggg gcgtcgaccc cctgatgacc gcgcccaggc
      961 atatgacacc gtccactgcg gcaaagaggg ccaccttctt ggccacggcc gggatctcga
     1021 aggcccccgg caccttgtag atcgtgatgt cttcggctgc cacgtcatga cggcccaggg
     1081 cgtcgagtgc cccctcgacc agcctcgccg tgacgaagtc gttgaacctg ctcgccacga
     1141 tcgcgacctt cttccccttg ccggcaagct tcccctctat gacctgcgcc atcacaccac
     1201 ctccaggatg tgtcccatct tttctttctt ggtcttgagg tacctctggt tctcgagggt
     1261 gggcgggatc tcgatcggga ccctctccac gatctccagg ccgtatccct cgagccctat
     1321 gatcttcttg gggttgttcg tcatgagccg catcttgcgc acccccaggt gaaggaggat
     1381 ctgggcgccg atgccgtaat ccctgaggtc cgcggggaag cccagctcca cgttcgcctc
     1441 cacggtgtcc tttcccttgt cctggaggtg gtaggccttg agcttgttga tgaggccgat
     1501 accccttccc tcctgcctca tgtacacgat cacgcccctg ccggcctcct cgatcatctc
     1561 catggcgcga tggagctgtg cgccgcagtc gcacctgagc gagtggaaga cgtcgcccgt
     1621 caaacactcg gagtgcacgc gcacgagcac gggctcttcg gggtcgatgg gccccttgac
     1681 gagggcgatg tgctccttgt cgtctatgtc gttgtcgaag cccacgagct cgaacgagcc
     1741 gtcctcggcc ggcagcttgg cccgcaccac ctcgcggacg agggtctccg tcctgatgcg
     1801 gtgggcgatg atgtcggcta tggtgcatat cttgaggccg aaggcgcggg cgaactcctc
     1861 gagctggggc ctgcgcgcca tggtgccgtc gtcgttcatg atctcgcaga tgacggcgcc
     1921 cggcttcagc ccggcgatgc ggcacaggtc gaccgagcct tccgtctgcc ccgtcctgac
     1981 gagcgtgccg cccggcctcg ccctgagggg gaacacgtgc ccgggccgcg cgaggtcgtc
     2041 aggccgggtg gccgggtcga tggccgtgcg gatggtgtgc gcccggtcgg ccgccgaaat
     2101 ccccgtggtg acccccctct tcgcctcgat ggagacggtg acacccgtcc cgaaccggct
     2161 cgtgttgtcg cgtaccatgg gctgcaggtc cagccggtcg gcgatctcgg gtgcgagcgc
     2221 caggcagatg agaccccttc cgtgcttggc catgaagttg atggcctcgg ccgtgacgaa
     2281 ctcggccgcc atggtgagat cgccctcgtt ctcccggtcc tcgtcgtcca ccaggatgat
     2341 catgcgaccc tgctggagct cgtaaaccgc ctcttccacg gtgcacgtcg gcatgtgtgt
     2401 cactccctgc gcttgaagcc cgcctgttcg agaagggtct cgattcctcc aacccctctc
     2461 tttttcagca gattttcgac atacttcccc aggatgtcga actcgatgtt caccctaaca
     2521 cccgcaccct tgcctgtcaa ggaagtggac ccggcggtgt gggggatgat gttgaccgtg
     2581 aacgcatccc ccagcgcctc gttgacggtc agctatatcc cgtccacggc cacggagccc
//
LOCUS       DAOSCR010000396         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25281_length_2638_cov_3.385598, whole genome shotgun sequence.
ACCESSION   DAOSCR010000396 DAOSCR010000000
VERSION     DAOSCR010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25281_length_2638_cov_3.385598"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLT69_09450"
     CDS             <1..558
                     /locus_tag="PLT69_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14703.1"
                     /translation="IKGPQVMKGYYKNPEATKEAFTEDGFIRTGDIGEIDKDGYLKIT
                     GRLKDIIVTSGGKNISPQNIENTLVTSPFIEQVAVIGDNRKFLSALIVPSFGTLEAWA
                     KENNIPFSSRKDLINNPEVYKLYEKEIEGLMKDFGKVEQIRKFTLLETEWSQETGELT
                     PTMKVKRKVINQKYAAVIDAMYPKD"
     gene            complement(1453..1662)
                     /locus_tag="PLT69_09455"
     CDS             complement(1453..1662)
                     /locus_tag="PLT69_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOJ14704.1"
                     /translation="MKYGARNTIKAKVKSVHKGDIMTLVKFDAISAPEMASVLTTESV
                     EGMELKPGDSVQLVIKAIHVLPVKE"
     gene            complement(2493..>2638)
                     /locus_tag="PLT69_09460"
     CDS             complement(2493..>2638)
                     /locus_tag="PLT69_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOJ14705.1"
                     /translation="NDARKGRSQGGDIVSAMVKSAAHAIGSQVGRQIIRGVLGSIFGG
                     RRR"
BASE COUNT          568 a          826 c          740 g          504 t
ORIGIN      
        1 atcaagggcc cgcaggtcat gaagggctac tacaagaacc ccgaggccac caaggaggcc
       61 ttcaccgagg acggtttcat ccgcaccggc gacatcggcg agatcgacaa ggacggctac
      121 ctcaagatca ccggccgcct caaggacatc atcgtgacct caggcggcaa gaacatctcg
      181 ccccagaaca tcgagaacac cctggtcacg tcacccttca tcgagcaggt ggcggtcatc
      241 ggcgacaacc ggaaattcct ctccgcgctc atcgtgccct ccttcgggac cctcgaggca
      301 tgggccaagg agaacaacat ccccttctcc agccggaaag acctcatcaa caaccccgag
      361 gtgtacaagc tctacgagaa ggagatcgaa gggctcatga aagacttcgg aaaggtcgag
      421 cagatcagga agttcaccct gctcgagacg gagtggtccc aggagaccgg cgagctcacc
      481 cccaccatga aggtgaagag aaaggtcatc aaccagaagt acgcggcggt catcgacgcc
      541 atgtacccga aggactgagg cccggccagg atacgaacaa ggagctcgat ccctcaagcg
      601 ttcggcggga cgacgacccg ccgaacgctt ttcttccgtc ggacgttccg ggcagggcgc
      661 ctgaggacgt caggtgcctg ccggcttcac gctgtctggc aaaaaatccg gcatcgatgg
      721 ggtgagtctc ggcggggcat tccattcgtt tcacaaggat cgcctcaaag tactgggcgg
      781 caatctgacc ctcgcagcga caagagaacg cgtccccctc tcatctctgc tatcaagatt
      841 cctgccggtg aacgtggtgg ccatgactgg caggaagatg caaagggcac gaggcctctg
      901 tcatgagaga gggtaccatg cccgacattg gcaacgtaga ggtagagccc ctggtctgtg
      961 accacgatgg aatcctcgca gaagacggca aaccgatccc atggcgttcg ttcctctccc
     1021 gctgcgctcg cacccggcag atggcctcat gcggccacgg ccgacacctt aagtccaaag
     1081 gcaaaccagc ttgacagatc gccggtgagg ctgaccggta taactgttgt gtccaagaca
     1141 cacatcacgt tcgttcgtgt atccctgctc tggtccaaga ccttgatcgc cacctgcaag
     1201 aacatcaacg atcgggatat cctgaactgg gctgctgccg gcagtacgct gggccgaaag
     1261 agggggtgtc cgttcaaaca ccggcacgtg cagtcatggt gacgtgagat atcaccgtag
     1321 ccctcgaaat ccaaaacatc ttgccgccga acagcatggt ggacacgctg ttcttgggat
     1381 catcctgact cgaaatccga aacgtcttgc cgctcagccc cgttcgtgag gggcgcatgc
     1441 gtgacgcgtg tgttactcct tgaccggcag cacgtggatg gccttgatga cgagctgcac
     1501 gctgtcgcct ggcttgagtt ccataccttc gaccgattcc gtggtaagga ccgacgccat
     1561 ttccggagcg cttatggcat caaacttcac cagggtcatg atgtcgccct tatgaacgga
     1621 tttcactttg gctttaatag tgttgcgtgc gccgtacttc atggtttcct ccttccccgc
     1681 aaacagggtt cataattcat ctgaccaaag ataactcagg acatccacaa atcaacacga
     1741 gggacatcaa gttcctgtaa gtatcatgag taagctgccc atgtggtgcc cgtcggatcg
     1801 caatccttcg agacgatcgt tggctgcgga gaagggtcct tgttcctgtg ccagcggtaa
     1861 aaccctcgat catgcgccac ggtaccgaca tcgtcgtttt ccagaagatg ggtgtatcgt
     1921 gtctgccgag gtcgggatca tcgaaatctg ggcggtgtcg gcctgagaca tcgccagctt
     1981 cacgggcctc atgtctctct cctcggtgtc cggcgcgacc acgcctgggc ggagaaagag
     2041 cggaggtgca ccttgccggc atcgaagggc atacagggcc tttcgtctcc tgccgtccat
     2101 acagttccag gcatcgccgc tacgaggatc acgctttgtg accggtgcgc ggaaatgggc
     2161 atcacgccag acatccccgc tacgaggatc acgcggcgta cgtgtggtcc aggcctggcc
     2221 tgcgaaccag acatccccga cacgaggatc acgcacatca cgatccgcac gagcgccctt
     2281 tccagaaagg ggctccatcg ggggctctct tctccctccg agacgggatc ggcgcaagct
     2341 atcgttccgc aggccccgca tatgtccgta tggtatggct ctcgacacgc actcctacgg
     2401 aagcctgtgg aatgtgtaca gccacatccg tcaccgatgc ggccattcat gtcgggcgac
     2461 gcggtgcccg gtcctgcatc ccaacagggg cctcaccgcc gcctgccgcc gaagatcgag
     2521 ccgagcactc ccctgatgat ctgcctgccc acctggcttc cgatggcatg ggccgcgctc
     2581 ttcaccatgg cggacacgat gtcaccgccc tgggatcgcc ccttcctcgc atcgttgg
//
LOCUS       DAOSCR010000397         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25380_length_2631_cov_3.486025, whole genome shotgun sequence.
ACCESSION   DAOSCR010000397 DAOSCR010000000
VERSION     DAOSCR010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25380_length_2631_cov_3.486025"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLT69_09465"
     CDS             complement(<1..459)
                     /locus_tag="PLT69_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ14706.1"
                     /translation="MKIGYKWIREFVDLDAGALEAAKALTMSGTEVEGAVRQGVPGQV
                     VCARILEAHRHPNADKLFVCTVDAGSGPLQVVCGAPNTREGMTSAFAPIGTDLGGGFV
                     VKKAKIRGVESFGVLLSEREIGLTDDHTGIMEIEAVCEPGAPLGEALDLED"
     gene            complement(476..1480)
                     /gene="pheS"
                     /locus_tag="PLT69_09470"
     CDS             complement(476..1480)
                     /gene="pheS"
                     /locus_tag="PLT69_09470"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ14707.1"
                     /translation="MEKQLEELQRTFLEDLESIRDPEQVEQARIAYLGRKGRLTTILR
                     QMGSLSPEERPRIGALANSIKAKMEQALAEVSARIASRQSVQGIDVTLPGRVLRRGSV
                     HVINQVMAEIEDIFRDLGFQVASGPEVEDDYHNFEALNIPENHPARDMQDTFYFGPGV
                     LLRTHTSPVQIRVMESQGLPVRIIAPGKVYRCDFDITHSPMFHQVEGLWVEEGITFAH
                     LKGVLYEFAHRCFGPEVPLRFRSSFFPFTEPSAEVDMGCIICRGAGCRVCGNTGWLEI
                     LGCGMVDPEVYRFVGIDPHVVTGFAFGMGVERIAMIKYGIDDIRLFYDGDLRFLLQFA
                     "
     gene            complement(1480..1827)
                     /gene="rplT"
                     /locus_tag="PLT69_09475"
     CDS             complement(1480..1827)
                     /gene="rplT"
                     /locus_tag="PLT69_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004087738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOJ14708.1"
                     /translation="MRVKRGVVGKRKRTRVHKLTQGYYGGRRNLVRAAQEAMRKGLAY
                     AYRDRRQRKRDFRRLWIARISAALRQRGLTYSKFIHALRQANVELDRKVLADMAVTDP
                     KGFSAVVESVTKA"
     gene            complement(1845..2039)
                     /gene="rpmI"
                     /locus_tag="PLT69_09480"
     CDS             complement(1845..2039)
                     /gene="rpmI"
                     /locus_tag="PLT69_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOJ14709.1"
                     /translation="MKLKTNRSAAKRFKATGTGKIKRNKAYARHLLSSKTTKQKRKLR
                     KSALADSANIAGIKQMLPYL"
     gene            complement(2059..2592)
                     /gene="infC"
                     /locus_tag="PLT69_09485"
     CDS             complement(2059..2592)
                     /gene="infC"
                     /locus_tag="PLT69_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOJ14710.1"
                     /translation="MGKYGFRQEEKDRIRVNDRIVAESVRVISPDGQQLGIMTVPAAL
                     NAAQDYGMDLVEVAPNATPPVCRIMEYGKYKYEKSKREAQARKKQTVIVIKEIKFRPK
                     TDEHDYQFKLKHIKRFLEDRNKVKVVIRFRGREIMHLDKGLEMLNRVREDCAEMANVE
                     MEPKAEGRIMVMMLAPK"
BASE COUNT          407 a          899 c          767 g          558 t
ORIGIN      
        1 gtcctcgagg tcgagagcct ctccgagcgg cgccccgggt tcgcagaccg cttcgatctc
       61 catgatgccg gtgtggtcgt cggtgagccc gatctcgcgc tcggagagca ggaccccgaa
      121 cgactcgacc cccctgatct tcgccttctt cacgacgaag ccgccgccca ggtccgtccc
      181 gatgggcgcg aacgcgctcg tcatgccctc cctcgtgttg ggcgcaccgc acaccacctg
      241 cagcgggccc gagcccgcgt ccaccgtgca cacgaacaac ttgtcggcgt tcgggtgcct
      301 gtgcgcctcc aggatgcgcg cgcacacgac ctgtccgggc acgccctggc gcaccgcacc
      361 ctcgacctcg gtgccgctca tggtgagggc tttcgccgcc tcgagggcgc ccgcatcgag
      421 gtcgacgaac tcccttatcc acttgtagcc gatcttcatg gttcgacccc ccgcatcagg
      481 cgaactgcag aaggaaccgc aggtcgccgt cgtagaagag ccggatgtcg tcgatgccgt
      541 acttgatcat ggcgatccgc tccaccccca tgccgaaggc gaaaccggtc acgacgtgcg
      601 ggtctatccc cacgaaccgg tacacctcgg ggtccaccat gccgcacccg aggatctcga
      661 gccagccggt gttcccgcac accctgcagc cggcgcccct gcagatgatg caccccatgt
      721 ccacctcggc ggagggctcg gtgaacggga agaacgacga ccggaacctg aggggcacct
      781 cgggcccgaa gcacctgtgg gcgaactcgt agaggacccc cttcaggtgc gcgaacgtga
      841 tgccctcctc cacccagagc ccttcgacct ggtggaacat cgggctgtgc gtgatgtcga
      901 agtcgcaccg gtagaccttg ccgggggcta tgatgcgcac cggcaggccc tggctctcca
      961 tgaccctgat ctggaccggc gaggtgtgcg tcctcaggag gacgccgggg ccgaagtaga
     1021 acgtgtcctg catgtcccgc gccgggtggt tctcggggat gttgagggcc tcgaagttgt
     1081 ggtaatcgtc ctccacttcc gggcctgacg ccacctggaa accgagatcc ctgaaaatgt
     1141 cctcgatctc ggccatgacc tggttgatga cgtggaccga gccccgcctg agaaccctgc
     1201 cgggcagcgt cacgtcgatc ccctgcacgg attgccgcga ggctatcctg gccgagacct
     1261 cggcgagcgc ctgctccatt ttcgccttga tgctgttggc cagcgccccg atgcgtggcc
     1321 tctcctcagg ggagaggctc cccatctgcc tcaggatggt ggtgagcctc cccttgcggc
     1381 cgaggtaggc gatcctggcc tgctcgacct gctcggggtc ccgtatggat tccaggtctt
     1441 cgagaaaggt ccgttggagt tcttcgagct gtttttccat cacgccttgg tgacagactc
     1501 gacgacggcg ctgaacccct tgggatcggt gaccgccatg tccgccagca ccttcctgtc
     1561 gagctccacg ttcgcctgtc tcaaggcgtg gatgaatttc gaataggtca agccgcgctg
     1621 cctgagcgcc gcgctgatcc tggctatcca gaggcgcctg aagtccctct tgcgctggcg
     1681 cctgtcccgg tatgcatacg cgaggccctt gcgcatcgcc tcctgggccg ccctgacgag
     1741 gtttctcctg cccccgtaat agccctgggt gagcttgtgc acccgcgtcc tcttcctctt
     1801 gccgaccact ccacgtttca ctcgcatggt acgcgctcct tgtcctacag gtacggcagc
     1861 atctgcttga tgcccgcgat gttcgcggaa tcggccaggg cggacttcct gagcttcctc
     1921 ttctgcttgg tcgtcttgga cgagaggagg tggcgggcgt acgccttgtt cctcttgatc
     1981 ttgcccgtgc ccgtggcctt gaatctcttt gcagcactgc ggttcgtctt cagtttcatc
     2041 gcctcagctc cttgggtgtc acttgggggc caacatcatg accatgatac ggccttcggc
     2101 cttgggctcc atctcgacgt tcgccatctc ggcgcagtcc tcgcggaccc ggttgagcat
     2161 ctccagaccc ttgtccaggt gcatgatctc cctgcccctg aaccggataa ccaccttcac
     2221 cttgttcctg tcctcgagga accgcttgat atgcttgagc ttgaactggt agtcgtgctc
     2281 gtcggtcttg ggacggaact tgatctcctt gatgacaatg accgtctgtt tctttcgggc
     2341 ctgagcctcc cgcttggact tctcgtactt gtacttgccg tattccatga tacggcagac
     2401 cggaggggtc gcgttcgggg cgacctcgac caggtccatc ccatagtcct gggccgcgtt
     2461 caaggcggcc ggcacggtca tgatcccaag ctgctgaccg tcgggggaaa tgaccctcac
     2521 gctctcggca acaatgcggt cgtttacccg gatcctgtcc ttttcctctt gtcgaaagcc
     2581 gtacttgcct atgcgaccct ccccgaaagt aagttatgcg cttatctaac c
//
LOCUS       DAOSCR010000398         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25565_length_2619_cov_3.336973, whole genome shotgun sequence.
ACCESSION   DAOSCR010000398 DAOSCR010000000
VERSION     DAOSCR010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25565_length_2619_cov_3.336973"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLT69_09490"
     CDS             complement(<1..335)
                     /locus_tag="PLT69_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14711.1"
                     /translation="MLGRVVRYFSSMGGKYGVEWWILCAGAALVGAIVLMAVFSGYIA
                     PFDPYDQAAGPQLSPPGPTHLMGTDNLQRDVFSRIVFGSQTILKVSVMAAIISSLVGI
                     TMGLVSGYAG"
     gene            complement(376..1392)
                     /locus_tag="PLT69_09495"
     CDS             complement(376..1392)
                     /locus_tag="PLT69_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ14712.1"
                     /translation="MGHLIRYIVARTLMTVPMVLILLTIIFAVLRLMPGDPVSAMLGG
                     HAPEHVIELKREQLGLNRPLHVQYVDYLWSVCRFDLGDSMVLQQKVTDIIAEKLGATI
                     ELTVFGMLVTVVVGVFLGAYAADRRRTLGDSAIRLYGIVVYCIPVYWLGLMLQLVFGV
                     WLDWLPIAGRTGPRVFPSDFERTGFYLVDTLLAGDTESFFDVLVHLVLPAFTLGLTLS
                     GIFVRLTRANMLDVLKSDYILAAESRGIPRRRVVYRHALKNALIPILTMMGLQVALLM
                     AGAVLTETTFSWPGMGRLLLERIYLRDYPTIQGVIIVFALMVAFISLVVDIVYAVVDP
                     RIRY"
     gene            complement(1464..>2619)
                     /locus_tag="PLT69_09500"
     CDS             complement(1464..>2619)
                     /locus_tag="PLT69_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ14713.1"
                     /translation="EVVDPLRVKFVLKNPVSYFPSLVATVPYYPLNPKVYPKDRLFRD
                     PSELKGGAMVGLGPYKVVSFKRDEEVVLEANPSFYGEKPLNKRVVIRYFADATTMRLA
                     LEKGEIDFAFKSLNPADIRDLQRSSAIQTIKAQGPYIRYLCFRCTGKPFDDKVLRQAV
                     AAAISRKPLLDKVYLGQNEPLYSMVPMGMWSHEDSFKAYPGDGNVALVKKLLKSRGYD
                     EKNKLAFELWYTPSHYGDTEVDLAAVLKQQLEATGVMKVEVKSAEWATYRDNWTNKAM
                     PAFLLGWYPDYIDPDNYTAAFAGTEGSKGMGIFFSNKAWDEMFSKAQKETDLKKRTEL
                     YKKIQKMWTDEVPTVPIFQGTLYVLARKGVEGIMLSPTLQFNYGPIHRTK"
BASE COUNT          482 a          816 c          832 g          489 t
ORIGIN      
        1 cccgcatagc ctgacaccag gcccatggtt atgcccacga gcgacgagat gatcgcggcc
       61 atgaccgaca ccttcaggat ggtctgggag ccgaacacga tcctggagaa cacgtcccgc
      121 tggaggttgt cggtgcccat gaggtgtgtg ggtcccggcg ggctgagctg cggcccggcg
      181 gcctggtcgt acgggtcgaa cggggcgatg taccccgaga agaccgccat gagcacgatc
      241 gcccccacaa gggccgcccc tgcgcacagg atccaccact ccacgccgta ctttccgccc
      301 atggaggaaa agtacctgac gacgcgtccg agcaagcctg aaggagaccc gttcatcccg
      361 gctgcgcccc ccttctcagt acctgatgcg cgggtcgacg accgcgtaca cgatatccac
      421 cacgagcgag atgaacgcca ccatgagggc gaagacgatg atcacgccct ggatcgtggg
      481 gtagtccctg aggtagatcc tctcgaggag gagcctgccc atgccgggcc acgaaaaggt
      541 cgtctcggtc agcacggcgc cggccatgag cagggcgacc tggagcccca tcatggtgag
      601 tatggggatg agcgcgttct tgagcgcgtg cctgtagacc acgcggcgtc tcggtatccc
      661 cctggactcg gcggcaagga tgtagtccga cttgaggacg tccagcatgt tcgccctggt
      721 cagcctgacg aagatgccgg acagcgtgag ccccagggtg aaggccggca acacgaggtg
      781 caccagcacg tcgaagaacg actcggtatc gccggcgagg agcgtgtcga cgaggtagaa
      841 gcccgtcctt tcgaaatccg acgggaagac cctggggcct gtccttcctg cgatgggcag
      901 ccagtcgagc cagacgccga agacgagctg gagcatgagg ccgagccagt acacggggat
      961 gcagtagacc acgatgccgt agagcctgat agccgagtcg ccgagcgtcc tgcgcctgtc
     1021 ggccgcgtac gcgccgagga acaccccgac aaccacggtc acgagcatgc cgaacaccgt
     1081 gagctcgatg gtggctccaa gcttctcggc gatgatgtcc gtcaccttct gctggagcac
     1141 catggaatcg ccgaggtcaa acctgcagac gctccagagg tagtcgacgt actggacgtg
     1201 aaggggccgg ttcaggccga gctgttcccg cttgagctcg atcacgtgct cgggtgcatg
     1261 cccaccgagc atggccgaga cagggtctcc gggcatgagc ctgaggaccg cgaatatgat
     1321 ggtgaggagg atcaagacca tgggcacggt catgagcgtg cgtgcgacga tgtacctgat
     1381 cagatggccc atgtcccttc tccgtccgta tcttcccggc ctcttctgtt aaagggcgcc
     1441 ccgaagggcg ccccccggtc ccgctacttg gtcctgtgta tggggccgta gttgaactgg
     1501 agcgtcggcg agagcatgat cccctcgacg cccttcctcg cgagcacgta gagggtgccc
     1561 tggaagatgg gcacggtcgg cacctcgtcg gtccacatct tctggatctt tttgtacagc
     1621 tcggtgcgct tcttgaggtc ggtctctttc tgggccttcg agaacatctc gtcccatgcc
     1681 ttgttcgaga agaagatgcc catgcccttg gacccctcgg tccccgcgaa ggcggcggta
     1741 tagttgtccg ggtctatgta gtcggggtac cacccgagga ggaaggccgg catggccttg
     1801 ttcgtccagt tgtcgcggta ggtggcccac tcggcggact tcacctcgac cttcatcacc
     1861 ccggtcgcct cgagctgctg cttcaagacc gccgcgaggt ccacctcggt atcgccgtaa
     1921 tgggagggcg tgtaccagag ctcgaaggcc aacttgttct tctcgtcata tcccctcgac
     1981 ttgaggagct tcttgaccag cgcgacgttc ccgtccccgg ggtatgcctt gaagctgtcc
     2041 tcgtgcgacc acatgcccat ggggaccatg gagtacagag gctcgttctg gccgaggtac
     2101 accttgtcca gaaggggctt cctgctgatt gcagccgcga cggcctgcct gaggaccttg
     2161 tcgtcgaagg gcttccccgt gcagcggaag cagaggtacc tgatgtacgg gccctgcgcc
     2221 ttgatggttt gtatcgcgct cgatctctgg agatccctga tgtccgccgg gttgagcgac
     2281 ttgaaggcga agtcgatctc acccttttcg agcgcaagcc gcatggtcgt tgcgtctgcg
     2341 aagtaacgga tcaccaccct cttgttgagc ggtttctccc cgtagaagct cgggttcgcc
     2401 tccaggacca cctcctcgtc acgcttgaag gacaccacct tgtacgggcc caggcccacc
     2461 atggcaccac ccttgagctc ggacgggtcg cgaaagagcc tgtctttggg gtacaccttc
     2521 gggttcagcg ggtagtaggg cactgtcgcc acgagcgagg ggaagtagga aacggggttc
     2581 ttgaggacga acttcacccg gagcgggtcg accacctcg
//
LOCUS       DAOSCR010000399         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25621_length_2615_cov_2.952734, whole genome shotgun sequence.
ACCESSION   DAOSCR010000399 DAOSCR010000000
VERSION     DAOSCR010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25621_length_2615_cov_2.952734"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLT69_09505"
     CDS             <1..296
                     /locus_tag="PLT69_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14714.1"
                     /translation="LRGVCEMARVLRPGGLLIVSETDPCASRLEIGLFAKGFRSWYLA
                     NGLALWFLEHVVFGHEYQAERLVSLCRQAGVEASESARVEGIPYVIVAGVKRP"
     gene            complement(599..1747)
                     /locus_tag="PLT69_09510"
     CDS             complement(599..1747)
                     /locus_tag="PLT69_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOJ14715.1"
                     /translation="MEFSLSEEHKMVRDMARRFADHELKPRAAEIDQTHRHNDEVLRK
                     MAENGFMGVAIPAEYGGAGMDYVAYALILTEISRGCASTGVIMSVNNSLYGFPVYTFG
                     TEEQRLKFNTPVASGAAHGCYGLTEANAGSDPASMKTSAVLDGNYWVINGEKKFITNG
                     NIAKYAVIAAVTDKTKGYKGITQFVIDLEETEGFSVGKVEDKLGIRGSGTSELVFQDA
                     RIPKDSILGDPGSGFKQMLTTLDSGRIGIASQALGIAKCCLEEAVKYSKERVQFGKPI
                     SEFQAIQFKLADMATRIDAAELLVLRAAWLESNHLPFEKEAAMAKMYASDVAMWATVE
                     GVQILGGYGYVTEYPMERLMRDAKITQIYEGTNEIMRVVVARNVLGKR"
     gene            1951..>2615
                     /gene="serC"
                     /locus_tag="PLT69_09515"
     CDS             1951..>2615
                     /gene="serC"
                     /locus_tag="PLT69_09515"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOJ14716.1"
                     /translation="MRNVFIFNPGPAVLPEPVLESTSKAVLDFAGTGMSIMEVSHRSK
                     QFDALLVETVNLVKKAMEIPDNYKVLFLQGGATMQFAMVPINLLAEGRVADYVDTGYF
                     ASRAIKEAKLIGKVNVAASTKDDNYTRIPRASELKLTKDAAYCHITSNNTIYGTQWQT
                     FPETGDVPLVADMSSDILSRRIDVKKFGIIYAGAQKNMGPAGVTCVILREDLIGRAPE
                     NTP"
BASE COUNT          447 a          853 c          823 g          492 t
ORIGIN      
        1 gcctccgggg cgtatgcgag atggcaaggg tcctcaggcc gggcggcctg ctcatcgtgt
       61 cggagaccga tccctgtgcg tcacggctcg agatcggcct cttcgccaag ggttttcggt
      121 catggtacct cgcgaacggc ctggcgctgt ggttcctcga gcacgtcgtg ttcgggcacg
      181 agtatcaggc ggagaggctc gtctcgctgt gcaggcaggc gggggtggag gcttccgagt
      241 ccgctcgcgt cgagggtatc ccgtacgtga tcgtcgcggg agtaaaaagg ccgtgaggga
      301 aaccgacacc gaggggttga ggccgagagc ccgggcaggg accgacgggg ggaaaacggc
      361 cgcagggaaa gcgtctgcct ggccgcgggc cctgccgcgc tcgaagacct tgcccctcga
      421 ggggctcacc gccggcgtcc agggcgaacc tcgcgcggtt caaggccatg ggccaggcct
      481 tgacggatcc atggggtggt tccgtgcccg aggccttgtc caccccgctc ggcggccatg
      541 aagaaagacg gccggggcgc cacgtgcccc ggccggtgga cgtgctcttc gtgcagggct
      601 acctcttgcc caggacgttc ctcgccacaa ccacccgcat gatctcgttg gtgccctcgt
      661 agatctgggt gatcttggcg tcccgcatga gcctttccat ggggtactcg gtcacgtatc
      721 cgtagccgcc gaggatctgc acgccctcca ccgtggccca catcgccacg tcggaggcgt
      781 acatcttggc catggcggcc tccttctcga agggcaggtg gttgctctcg agccaggccg
      841 ccctcagcac gagcagctcg gccgcgtcga tcctggtcgc catgtcggcg agcttgaact
      901 ggatggcctg gaactcggat atgggctttc cgaactggac gcgttccttg gaatacttca
      961 cggcctcctc gaggcagcac ttcgcgatgc cgagcgcctg cgaggcgatg cctatgcgtc
     1021 ccgagtccag cgtggtgagc atctgcttga agccgctgcc agggtcaccc aagatcgagt
     1081 ccttggggat cctcgcgtcc tggaacacga gctcggaggt gccggagcca cggataccga
     1141 gcttgtcctc cacctttccc accgagaagc cctcggtctc ctcgaggtct atcacgaact
     1201 gggtgatgcc cttgtagccc ttggtcttgt cggtcacggc ggcgatcacc gcgtactttg
     1261 cgatgttgcc gttggtgatg aacttcttct cgccgttgat gacccagtag ttgccgtcga
     1321 gcacggcgct cgtcttcatg ctcgcgggat ccgagcccgc attcgcctcg gtgagcccgt
     1381 agcacccgtg agcggccccg gaggcgaccg gcgtgttgaa cttcaggcgc tgttcctcgg
     1441 tgccgaaggt gtagacggga aacccgtaca gggagttgtt gaccgacatg atcacgcccg
     1501 tgctcgcgca gcccctggat atctcggtga ggatgagagc gtatgcgacg tagtccatgc
     1561 cggcgccgcc gtactcggcc gggatggcca cgcccatgaa cccgttctcg gccatcttcc
     1621 tgaggacctc gtcgttgtgc cggtgggtct ggtcgatctc ggcggccctg ggcttcagct
     1681 catggtctgc aaacctcctc gccatgtcgc gtaccatctt gtgctcttcg ctcagcgaaa
     1741 actccataac gatcctcctg gtctgagtgc gtcaaccatc gcccgggccg agccgtcgac
     1801 ggcggccccg aatgaatgag cgttcattta tatacgcctt ctcacgcccg ctgtcaacgc
     1861 gatgcgagcc acgccgtcct ttacccaggg tcattttgct gatatatagg tagggtttca
     1921 tcacccacgc gaaggaggct tgctgaggct atgagaaacg tcttcatctt caaccccggt
     1981 cccgcagtcc ttcccgaacc ggtcctcgag agcacgagca aggccgtgct ggacttcgcc
     2041 ggcacgggca tgtccatcat ggaggtgtcc caccgatcca agcagttcga cgcgctcctc
     2101 gtcgagacgg tgaacctcgt gaagaaggcc atggagatcc cggacaacta caaggtcctc
     2161 tttctccagg gcggcgcgac catgcagttc gccatggtcc ccatcaacct cctcgccgag
     2221 ggccgggtgg cggactacgt ggacacgggc tacttcgcct cgcgcgccat caaggaggcg
     2281 aagctcatag ggaaggtcaa cgttgcggca tcgaccaagg acgacaacta cacgaggatc
     2341 cccagggcgt ccgagctcaa gctcaccaag gacgccgcct actgccacat cacctccaac
     2401 aacacgatct acggcaccca gtggcagacc ttcccggaga ccggcgacgt cccgctcgtg
     2461 gcggacatgt cctcggacat cctctcgcgg aggatcgacg tgaagaagtt cggcatcatc
     2521 tacgcaggcg cccagaagaa catggggccc gcgggcgtca cctgcgtgat cctcagggag
     2581 gacctgatcg gcagggcgcc ggagaacacg cccca
//
LOCUS       DAOSCR010000400         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25678_length_2610_cov_4.011350, whole genome shotgun sequence.
ACCESSION   DAOSCR010000400 DAOSCR010000000
VERSION     DAOSCR010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25678_length_2610_cov_4.011350"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLT69_09520"
     CDS             complement(<1..719)
                     /locus_tag="PLT69_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ14717.1"
                     /translation="MSMVAFIGWRGMVGSVLMDRMIAEGDFKGFDAVFSSTSQVGLEG
                     PDVGLDIPPLKDAYDIRELKKADIIVTCQGSDHTKEVYPRLRNDGWKGYWIDASSALR
                     MKDDSIIILDPVNRDVIDRGLHRGVKDFIGGNCTVSLMLMAIGGLFKSNLVEWVSSMT
                     YQAASGAGANNMRELVEQMAFVGDASRSLLERPATAALELDRKVMEAIGDRSFPKKVF
                     GYPLAANVLPWIDTEMENGQS"
     gene            1074..>2610
                     /locus_tag="PLT69_09525"
     CDS             1074..>2610
                     /locus_tag="PLT69_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14718.1"
                     /translation="MEMLTPGYMFAIPQLLTVLTGASMLVLVLGTGAMDAHKRIMAIV
                     SLLCVVDAGASVASKTASMIGVPFSLPGVVTLVKLFIPPLSVCLLQAAGRGRAMCTWQ
                     AVLVAATAVFISLSAGNALNPADTAPWFAVVILAGSVAPVVPGYLRGGGGPEKGLGPV
                     RIVTVAGVVSLAALSALDFFVEWPIPPMGFAFVGLIMAAVGLLLEGVNEEERVANQVR
                     LVTALLVASFFLAVSADLLFALTTPVTTGLSDTPVHLLWFAASSVASFFLCGACAAII
                     AKASKRSLVDAALLSAGALMGAILNLRDIMGVFYQGNLREIYLLNDLLLALSPGVVLH
                     LAERFTRKTPGREVIAFYAAGAVSAAVIMAESTTSLGPVVFGRGIGQYLFLGSLTAAL
                     SRSLWIVGAFAKAREVGAEKVNARYFLGGYAALGLIVLVSMITEVRFGLSAYFLAWVP
                     LGVVSWSVLAGAERGLGFSMGRHIPALAIRAAVSTIYVAAVAASYALLKGYPAEFILE
                     RLVP"
BASE COUNT          394 a          857 c          815 g          544 t
ORIGIN      
        1 ctgctctgtc cgttctccat ctcggtgtcg atccagggca gcacgttggc tgcgagggga
       61 tacccgaaga ccttcttggg gaacgaccgg tccccgatcg cctccatgac cttccggtcg
      121 agctcgagag cggccgttgc aggacgttcg agcagggacc tggaggcgtc gccgacgaag
      181 gccatctgct ccacgagttc ccgcatgttg ttcgcgcccg cacccgaagc ggcctggtag
      241 gtcatcgacg acacccactc gacgaggttt gacttgaaca gaccgcctat cgccatgagc
      301 atgaggctca ccgtgcaatt gccgccgatg aagtccttga cgcccctgtg caaccccctg
      361 tcgatcacgt cgcggttcac ggggtcgagg atgatgatgc tgtcgtcctt catgcgaagg
      421 gcggacgacg cgtcgatcca gtaacccttc cagccgtcgt tgcggagcct ggggtaaacc
      481 tccttggtgt ggtcgcttcc ctggcaggtg acgatgatgt ccgccttctt gagttcgcgg
      541 atgtcgtacg cgtccttgag cggcgggatg tccaggccca cgtccggacc ctcgagcccc
      601 acctgggagg tggaggagaa gacggcgtcg aagcccttga aatcgccctc ggcgatcatc
      661 ctgtccatga gcacggaacc caccatgccg cgccacccga taaacgccac catcgacatc
      721 cgtgatcccc cttctctgtg tgtcccttct tcccggccct tcacgtcgag agatccctcc
      781 ggccggggaa tatctatcta tcgcgcgcgg tgtaccccag tcaacaacgt cgatctcgcg
      841 gccggtcctg ctcctggata cgcgaggtgc acaagggcag agcgtcgatc gggtgagcgg
      901 cggcgccttc gcacggacga tgcgcattcc agccgggcac cccggcctca gggcgcacaa
      961 cctcccgcat gctaaggcct gggaagatgt aaacccggtt atcctcctca ccgataagga
     1021 tgcatatggc aggaaggaca cttctgcgtt caccctgacc gtggacggag agcttggaga
     1081 tgctcacacc cgggtacatg ttcgccatac cccagctgct gacggtcctc accggtgcat
     1141 cgatgctcgt gctcgtactg gggactggcg cgatggatgc acacaagagg atcatggcga
     1201 tcgtgagcct cttgtgcgtg gtggatgcag gcgcctcggt ggcgtcgaag acggcctcga
     1261 tgatcggcgt ccccttctct ctgccggggg tcgtcaccct cgtgaagctc ttcatcccgc
     1321 ctctttccgt ttgcctcctc caggccgcag ggagagggcg ggccatgtgc acgtggcagg
     1381 ccgtcctcgt cgcggccact gccgttttca tctcgctgtc cgcagggaac gcgctcaacc
     1441 cggcggacac agcgccatgg ttcgccgtgg tgatcctcgc gggttccgtg gccccggtcg
     1501 tcccgggata cctgaggggc ggtggcggcc ctgagaaagg ccttgggcct gtgcgtatcg
     1561 tcaccgtcgc cggggtcgtg tcgcttgcag ccctttccgc cctggatttc ttcgtggaat
     1621 ggccgattcc ccccatggga ttcgccttcg ttggactcat catggcggct gtcggcctcc
     1681 tcctcgaggg ggtcaacgag gaagagaggg tcgcgaacca ggtgcgactc gtgaccgcgc
     1741 ttttggtggc atcgttcttt cttgcggtgt cggccgacct cttgttcgcc ctcaccaccc
     1801 ccgtgaccac gggtctctca gatacgcctg tgcacctcct ctggttcgcc gcctccagcg
     1861 tggcgtcgtt cttcctgtgc ggcgcgtgcg ccgcgatcat cgcaaaggcc agcaaacgca
     1921 gtctcgtcga cgcggcgctg ttgtccgcgg gggcgctcat gggcgccatc ctgaatctca
     1981 gggacatcat gggggtgttt taccagggaa atctcaggga gatctacctc ttgaacgact
     2041 tgctcctcgc cctgtcgccg ggcgtggtgc tccacctggc cgaaaggttc acgaggaaga
     2101 ccccgggcag ggaagtgatc gccttctacg cggcgggtgc ggtgtcggcc gcggttatca
     2161 tggccgagtc gacgacctct ctcgggccgg tcgtgttcgg gaggggaatc ggtcaatacc
     2221 tgttcctcgg ctccttgacg gccgcgctct cccgctccct gtggatcgtg ggcgccttcg
     2281 caaaggctcg ggaggtcggc gccgagaagg tcaatgcacg gtacttcctg gggggatacg
     2341 ccgccctcgg cctcatcgtg ctcgtcagca tgatcaccga ggttcgcttc gggctctctg
     2401 catacttcct cgcgtgggtt ccgctgggtg tggtctcatg gtcggtcctg gccggggccg
     2461 agaggggcct cggcttctcc atgggccgcc acatccctgc cctcgccata cgtgcagctg
     2521 tctccaccat atacgtagcg gcagtggccg cttcgtatgc ccttctgaaa gggtatcccg
     2581 cggaattcat cctcgagagg ctggtcccct
//
LOCUS       DAOSCR010000401         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25713_length_2608_cov_3.593811, whole genome shotgun sequence.
ACCESSION   DAOSCR010000401 DAOSCR010000000
VERSION     DAOSCR010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25713_length_2608_cov_3.593811"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLT69_09530"
     CDS             complement(<1..207)
                     /locus_tag="PLT69_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOJ14719.1"
                     /translation="MTNREIVEALVFSCSGVCHVDDIERILPGLGADEIEKIVDDLNR
                     VYESTGRSFRIQRAASGYLFATKPE"
     gene            complement(204..971)
                     /locus_tag="PLT69_09535"
     CDS             complement(204..971)
                     /locus_tag="PLT69_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOJ14720.1"
                     /translation="MEEPVAVWDRGETPFVVSIQAFEGPLDLLLYLIERNRFTLQDVQ
                     VSPIVDQYLAYMERARRLDISLAGEFLDMASYLIWLKSRILLPKPEALQVEDESDPVQ
                     ELRDMLVAYRAVKAAAGSLGARPMLYWDTFPRGADTDEREITRASIAPLLEAVAAIRL
                     RTRKMVMDVMPKRYSLREIMAKIQSLFARRSRIRLLEAVEGMEKGSLIAALMAALEMS
                     RLNMVRIAQKGLFAPIFLLKRAPVVHAAGSPEQGEDE"
     gene            complement(996..1985)
                     /gene="trpS"
                     /locus_tag="PLT69_09540"
     CDS             complement(996..1985)
                     /gene="trpS"
                     /locus_tag="PLT69_09540"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ14721.1"
                     /translation="MDTRRIVSGIRPTGELHLGHYLGVLKNWVELQSDYQCFYFVADW
                     HALTSEYKDPEVIAQSTRDMVGTWLAVGIDPSRSVVFRQSDIKEHAELFTLLSMITPV
                     AWLERNPTYKEMREELKDKDLGTFGFLGYPVLQAADIVMYHADRVPIGQDQLPHLELT
                     REIVRRFSFLYGGGILKEPKEILTEAPKVPGLDGRKMSKSYGNAIFIAEDEKSLESKV
                     MQMLTDIKRMRRNDPGEPDDCNLYPFHEHFTDEKERERITAGCRDASWGCVECKRLLV
                     NNLIARFSPVWEKIAYYRSNTAIVDEVLAQGGARARAEAARTMERVRAAMKIS"
     gene            complement(2012..2599)
                     /locus_tag="PLT69_09545"
     CDS             complement(2012..2599)
                     /locus_tag="PLT69_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ14722.1"
                     /translation="MALLVLPVLLSITVHEVCHGYAAYLLGDPTAKAAGRLTFNPIKH
                     IDPVGMLVLFITRMIGWAKPVPVDPRYFKKPRRDMVWVSLAGPASNLVLAVIVAAALR
                     QAGPMLAATPLYPLVIMGQLTVMINVGLAVFNLIPIPPLDGSHILEGLLPERLAYSYA
                     QLRPYGFVILLLLVFTNVVDVVVGPVIEAIYSRLM"
BASE COUNT          449 a          849 c          792 g          518 t
ORIGIN      
        1 ctccggcttg gtggcgaaga ggtagccgga tgcggcgcgc tgaatcctga agctcctgcc
       61 ggtggattcg tacacgcggt tcaggtcgtc cacgatcttc tcgatctcgt ccgcaccgag
      121 tccgggcagg atcctctcga tgtcgtcgac gtgacagacg ccgctgcacg aaaacacgag
      181 ggcctcaacg atctcccggt tcgtcactca tcctccccct gctccggtga acccgcggca
      241 tgcaccaccg gagcgcgctt caggagaaag atcggggcga agagcccctt ctgtgctatc
      301 cgcaccatgt tcaggcgtga catctcgagt gccgccatca aggccgctat gagcgacccc
      361 ttctccatgc cctccaccgc ctccaacagg cggatccttg accgtcttgc aaagagagac
      421 tggatcttcg ccatgatctc cctcaagctg tagcgcttgg gcatgacgtc catgaccatc
      481 ttcctggtcc tcaggcgtat cgccgcaacg gcctcgagca ggggtgctat gctcgcgcgg
      541 gttatctccc tctcgtctgt gtccgcgccc ctggggaagg tgtcccagta gagcatgggc
      601 ctcgcgccca gcgagcccgc ggcggccttg acggcccggt aagcgacgag catgtccctg
      661 agttcctgga ccgggtccga ttcgtcctcc acctgaaggg cctcgggttt cggcaggagg
      721 atgcgggact tgagccagat gagatacgag gccatgtcga gaaactcgcc ggcaagcgat
      781 atgtccagtc tcctcgcacg ctccatgtat gcgaggtact ggtccacgat ggggctcacc
      841 tgcacgtctt gaagggtgaa acggttcctc tcgatgaggt agagcaacaa atcgagcggc
      901 ccctcgaagg cctgtatgct gaccacgaag ggggtttccc ccctgtccca caccgcgacc
      961 ggttcttcca tgcgccccct tcccctcctg tcgggtcagg agatcttcat ggccgccctg
     1021 acccgctcca tggtacgcgc ggcctcggcc ctcgcccttg ccccgccttg ggccagcacc
     1081 tcgtccacga tcgcggtgtt cgacctgtag taggcgattt tctcccatac cggggagaac
     1141 ctggcgatca ggttgttgac gaggagcctc ttgcactcga cacaccccca cgacgcgtcc
     1201 ctgcaccccg ctgttatacg ctcccgttcc ttctcgtccg tgaagtgctc gtggaagggg
     1261 tagaggttgc agtcgtccgg ttccccgggg tcgttcctgc gcatgcgctt gatgtccgtg
     1321 agcatctgca tcaccttgga ctcgaggctc ttttcgtctt ccgctatgaa gatggcgttg
     1381 ccgtaggact tcgacatctt cctgccgtcg agacccggca ccttgggcgc ctcggtgagg
     1441 atctccttcg gttccttcag gatcccgccg ccgtagagga aggagaacct cctcacgatc
     1501 tcccgcgtca gctccaggtg ggggagctgg tcctggccga tggggaccct gtcggcatgg
     1561 tacatgacga tgtccgccgc ctggagcacg gggtagccga gaaacccgaa ggtccccagg
     1621 tccttgtcct tgagctcctc cctcatctcc ttgtaggtcg ggttcctctc gagccaggcc
     1681 acgggcgtga tcatcgacag cagcgtgaag agctcggcgt gctccttgat gtccgattgc
     1741 ctgaagacca ccgacctcga ggggtcgatc cccacggcca gccaggtgcc caccatgtcg
     1801 cgggtgctct gggctatcac ctcggggtcc ttgtattcgc tggtgagcgc gtgccagtcc
     1861 gcgacgaagt agaagcactg gtagtcggac tgcagttcca cccagttctt gaggacgccc
     1921 aggtaatgcc ccagatggag ctcgcctgtc ggcctgatgc ccgatacgat gcgccttgtg
     1981 tccatggtcc cgtctttcct tcccttcatc ctcacatgag ccttgagtag atcgcctcta
     2041 tgacaggccc gacaaccacg tccaccacgt tcgtgaagac cagcaggagg aggatcacga
     2101 agccgtaggg cctgagctgc gcataggagt acgcgagcct ctcgggcagg aggccctcga
     2161 ggatatggct cccgtccagc gggggtatgg ggatgaggtt gaacacggcg agcccgacgt
     2221 tgatcatcac ggtcaactgg cccatgatga ccaacgggta caggggtgtc gcggcgagca
     2281 tgggcccggc ctgcctgagc gccgccgcca cgatgaccgc gagcacgagg ttggaggccg
     2341 gcccggccaa ggacacccaa accatgtccc tcctcggttt cttgaagtaa cgggggtcca
     2401 cgggaacggg tttcgcccag ccgatcatcc tcgtgatgaa gagcacgagc atgccaacag
     2461 ggtctatgtg cttgatgggg ttgaaggtga gcctgccagc cgcctttgcc gtcgggtcgc
     2521 cgaggagata ggcggcatac ccgtgacaca cctcgtgcac cgtgatcgag agcaggacgg
     2581 gcaggacgag caaggccagc tggcttgc
//
LOCUS       DAOSCR010000402         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_25750_length_2605_cov_3.541961, whole genome shotgun sequence.
ACCESSION   DAOSCR010000402 DAOSCR010000000
VERSION     DAOSCR010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25750_length_2605_cov_3.541961"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1132
                     /locus_tag="PLT69_09550"
     CDS             338..1132
                     /locus_tag="PLT69_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOJ14723.1"
                     /translation="MKKVNEGRTCSYTVNEQGVALMEVNNPPMNALSRPVIADMRDAI
                     TAALKDADVRVIVFTGVGKAFIAGADIGELKDFTTAQEGADFLIPGQEITNLLMYADK
                     PVIAAINGFCLGGGLEMALACHIRLADESAMLGLPEIKLGIIPGYGGTQRTPRLIGTG
                     RALELILSGNFIDGAKAAQYGLVNRAVAKGTVVEESMKLAAVIAAKSRIAVQAALRAV
                     HEGMSMDLHMAMKLERELFGICYASEDKLEGIAAFMEKRDPRFKDK"
     gene            complement(1336..>2605)
                     /locus_tag="PLT69_09555"
     CDS             complement(1336..>2605)
                     /locus_tag="PLT69_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ14724.1"
                     /translation="IQVNLAPKDERDRQSHEIAKAMRPRLVEIGRKFGADVKVVEVPP
                     GPPVMSTLVAEVYGPDLSGRIGIARRIREIFSHAEDVVDVDWYVADRQPPRLVYEVDR
                     VKALQSGVTQHAAATTLAAGLSEAVCGLVHLEREREPVEIALRLPERLRGDAASVLSM
                     SLPTPTGGFVSLSELVRPRQDTVPQTIYHKNMSPVTYVTGDVAGRLESPVYAILNMKD
                     EISRIRLPDGSALAQLWSDQPWLDTTYAMKWDGEWQITLEVFRDLGVAFAAVLVIIYI
                     LVVAWFKSFVTPLVIMAPIPLTLVGILPGHLIFGAFFTATSMIGFIALSGIVVRNSIL
                     LVDFILSEWTGANDLEDAIVSACTVRLRPIVLTAAAVVVGSFVMLFDPIFQGLAIAMM
                     FGAVASTLLTLGAVPLLFFVIFKNRPRPEA"
BASE COUNT          457 a          824 c          909 g          415 t
ORIGIN      
        1 tgaggggttt ggacgggtgg ttccttcggg aggcccccgg ctccagtgtg ctgtcttctc
       61 gggctgcgcc ggtcccggcg cgacggcccc ttgcgggcat ccccgtggct gcgccttgcc
      121 gtgggcccat ggccgggagg agcggtgttt ccatgaaggt ggagaggtgg tatgatacac
      181 atgcccggtc gaacgagcgg gttcgcacgc acgagccggg gcccaggcgg agggttggct
      241 ggacatgacc gcctccggcg ccccggacgc accgtctggc gcccgggatc gacgtgagcg
      301 tgcgggccct ggatatccgc ttgagaaagg agctgtcatg aagaaggtca acgagggcag
      361 gacgtgttcg tacacggtga acgagcaggg cgttgcgctc atggaggtga acaacccccc
      421 catgaacgcc ttgagcaggc cggtcatcgc cgacatgagg gatgcgataa ccgctgcgct
      481 caaggacgcg gacgtgaggg tcatcgtgtt cacgggcgtc ggaaaggcct tcatcgcggg
      541 cgccgacata ggcgagctca aggacttcac cacggcccaa gaaggcgcgg acttcctcat
      601 ccccggccag gagatcacga acctgctcat gtacgcggac aagccggtga tcgcggcgat
      661 caacggcttc tgcctgggcg gggggttgga gatggcactg gcctgtcaca tcaggctcgc
      721 ggacgagagc gccatgctcg ggctgccgga gatcaagctg ggcatcatcc cgggctacgg
      781 gggcacccag cgcacgccgc gcctcatagg taccgggcgg gccctggagc tcatcctgtc
      841 cgggaacttc atcgacggcg ccaaggcggc tcagtacggg ctcgtgaaca gggcggtggc
      901 caaggggacc gtggtggaag agtccatgaa gctcgcagcc gtcatcgcgg ccaagagcag
      961 gatcgccgtg caggcggcgc tcagggccgt tcacgagggc atgagcatgg acctccacat
     1021 ggccatgaag ctcgagcggg agctcttcgg catctgctac gcgagcgagg acaagctgga
     1081 gggcatcgcg gccttcatgg agaagaggga ccccagattc aaagacaagt gaagccgggc
     1141 tgcggcacga tgcgggtccc cgcgcccggg cgcacccttc tcgaagggcg ccgccccgtc
     1201 gggcagcctg cggtgcggtc gtgcaccaag gcgcacccct ctcgaggggg tgtgcgctcg
     1261 taagggcccg gccccctcgc gacggggtct ttgtgccagc gctcaccttc ccgtgtgcgc
     1321 acggaaggcg tcgcgtcagg cctcagggcg cggcctgttc ttgaatatca cgaagaagag
     1381 gagcgggacg gcacccaggg tcaagagggt cgaggccacg gcgccgaaca tcatggcgat
     1441 ggcgagtccc tggaagatgg ggtcgaagag catcacgaag gaacccacca ccaccgccgc
     1501 cgcggtgagg acgatggggc gcagcctcac cgtgcaagcg ctgacgatcg catcctccag
     1561 gtcgttcgcg cccgtccact cgctcagtat gaagtcgacg agcaggatcg agtttcgcac
     1621 cacgatgccg gacagggcga tgaagccgat catcgaggtc gccgtgaaga aggcgccgaa
     1681 gatgaggtgg ccgggcagga tgcccacgag ggtcaatggt atgggtgcca tgatgaccag
     1741 gggcgtgacg aagctcttga accatgccac aacgaggatg tagatgatca cgagcactgc
     1801 cgcgaacgcc acgcccaggt ccctgaacac ctccagggtg atctgccact cgccgtccca
     1861 cttcatggcg taggtggtgt cgagccaggg ttggtcggac cagagctgcg ccagtgccga
     1921 cccgtcggga agcctgatgc gcgagatctc gtctttcatg ttcaggatcg cgtacactgg
     1981 gctctcgagc cggccggcca cgtctcccgt cacgtaggtg accggcgaca tgttcttgtg
     2041 atagatggtc tgcggcacgg tgtcctgtct cggccgcacg agctcggaca gcgacacgaa
     2101 accgcctgtg ggggtcggca gggacatgga caggacactg gcggcgtcac ccctgagcct
     2161 ctccggcagg cgaagcgcga tctccacggg ctcgcgttcc ctctccaggt gcaccaggcc
     2221 gcagaccgcc tcgctcaacc cggcagccag ggtggtggcc gccgcgtgct gggtaacccc
     2281 gctctgcagc gccttgaccc tgtcaacctc gtagacgagc ctcggcggtt ggcggtctgc
     2341 gacataccaa tcgacgtcca ccacgtcctc ggcgtgggag aagatctccc tgatcctgcg
     2401 cgcgatcccg atccgccccg acaggtccgg gccgtagacc tctgcgacca gggtgctcat
     2461 caccgggggg ccgggcggga cctcgaccac tttcacgtcg gcgccgaact tccggccgat
     2521 ctcgacgagg cggggtctca tggcctttgc gatctcgtgg ctctgccggt cccgctcgtc
     2581 cttgggtgcg aggttgacct ggatg
//
LOCUS       DAOSCR010000403         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26055_length_2582_cov_2.878512, whole genome shotgun sequence.
ACCESSION   DAOSCR010000403 DAOSCR010000000
VERSION     DAOSCR010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26055_length_2582_cov_2.878512"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLT69_09560"
     CDS             complement(<1..677)
                     /locus_tag="PLT69_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOJ14725.1"
                     /translation="MARRTMIPSAAFALSTLAACFFLAAQPHPPAARAASTATAHASS
                     ARIPVFVSIVPQAYFVERVGGDLVDVEVLVGPGQSPHTYEPTPRQVARIARARVYFTI
                     GVPLEKGLVPKLARSFPGLSIVDTTSGIRFIPMDRRHEHPRTQAGGQGHDHGGYDPHV
                     WLDPGRVKIQASNICDALVRVDPAHAGVYRANLSAFLDDLDALDRELKEVFAPMKGSK
                     VYVFHPA"
     gene            complement(762..1016)
                     /locus_tag="PLT69_09565"
     CDS             complement(762..1016)
                     /locus_tag="PLT69_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOJ14726.1"
                     /translation="MKKQGIITFKVDDALYSIIREMPNRSEFIRGAILSALGNVCPLC
                     NGTGILSANQKQHWDEFCRDHAVMTCDECSERHLVCMTAK"
     gene            1099..1953
                     /locus_tag="PLT69_09570"
     CDS             1099..1953
                     /locus_tag="PLT69_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ14727.1"
                     /translation="MDKVLALMRRSAFLRENETGRVEGPGFSFHHLVRGAGRPVILVH
                     GGGVWLYSYRHIIAPLSGSLAVHALDMPGHGFTWRRDRGTALDLEVMAGALKEYTSLL
                     GIEKASLVGHSWGGAWVIAYALLYPEATDRIVLIDSSGLDVPDTRSWELLKIPFVGEA
                     LVTLAPTWAGRRMLSMAYADTGVLDREAARETLLPLKIPGNRKDLLNLCRNLSWKGLE
                     QGMRRLGCPAMLIWGEHDRYLDKGLAGRFRDLMEGLEVHVVQGSGHCPHEERPELTSR
                     LIAGFLCA"
BASE COUNT          435 a          845 c          852 g          450 t
ORIGIN      
        1 aaggctgggt ggaagacgta gaccttcgag cctttcatgg gcgcgaacac ctctttgagc
       61 tcgcggtcga gcgcgtcgag atcatcgagg aaggccgaaa ggttcgcccg gtacacccct
      121 gcgtgcgccg ggtcgaccct caccagcgcg tcacagatgt tcgaggcctg gatcttgacc
      181 cgcccggggt cgagccagac atgggggtcg tatccgccat ggtcgtggcc ttgcccgcct
      241 gcctgcgtgc gcgggtgctc gtggcgccga tccatgggga tgaagcggat ccctgatgtc
      301 gtgtctacga tggagaggcc cgggaaggac ctcgccagct tcggcacgag ccccttctcg
      361 agcggcacgc cgatcgtgaa atagaccctg gcacgcgcga tgcgcgccac ctgcctcggc
      421 gtcggctcgt aggtgtgggg cgactggccg gggccgacga gcacctcgac atccacaagg
      481 tctcccccga cgcgctccac gaagtatgcc tgggggacga tgcttacgaa gaccggtatc
      541 cttgcagagg atgcgtgggc tgtcgccgtc gatgccgccc gggcggcagg cgggtggggt
      601 tgggcagcaa ggaagaagca ggcggcaagg gtggaaagcg cgaaggcggc gcttgggatc
      661 attgtgcgac gtgccaccgt ctcaacctcc ctcgttttcc ccggggacct ctctgcggag
      721 cgcttccggc cactgcacga ggtaccgcca tatcgatccc ctcacttcgc cgtcatgcac
      781 acgaggtggc gctcgctgca ctcgtcgcac gtcatcaccg cgtgatcccg gcagaactcg
      841 tcccagtgct gcttctggtt cgcgctgagg atgcccgtcc cgttgcacag ggggcagacg
      901 ttgcccaggg cggaaaggat cgcacccctg atgaactcgg agcggttcgg catctccctg
      961 atgatggagt agagcgcgtc gtcaaccttg aacgtgatga tcccctgctt cttcataggc
     1021 ccctccatgc cgtgggtaat accaagtaat accacggccg tgttcctgtc aagcagcaac
     1081 gggatgtatc atcgcgccat ggacaaggtg ctcgccctga tgaggcgatc tgcgttcctc
     1141 agggagaacg agacaggccg cgtggaaggc ccgggctttt cctttcacca cctcgtccga
     1201 ggggcgggaa ggccggtcat cctcgtgcac ggcggaggcg tgtggctcta ctcgtacagg
     1261 cacatcatcg ccccgctctc gggctcgctc gccgtgcatg ccctggacat gccggggcac
     1321 ggcttcacct ggagacgcga ccgaggcaca gccctggacc tcgaggtcat ggcgggcgcc
     1381 ctcaaggagt acacgtccct cctgggcatc gagaaggcat cgctggtggg gcactcctgg
     1441 ggcggcgcgt gggtcatcgc ctatgccctt ctctaccccg aggctactga caggatcgtc
     1501 ctcatcgact cgagcgggct cgacgtcccg gacacgagga gctgggaact cttgaagata
     1561 cccttcgtcg gggaagcgct cgtgacgctt gcgcccacct gggcgggcag gcgcatgctg
     1621 tccatggcct atgcggacac cggcgtgctg gacagggaag cggcgaggga gaccctcttg
     1681 cccctgaaga tccccggcaa caggaaggac ttgctgaacc tctgcaggaa cctgtcgtgg
     1741 aaagggctcg agcagggcat gcgccgcctg gggtgtccgg ccatgctcat ctggggcgag
     1801 cacgacaggt acctcgacaa ggggctcgcg ggccgtttcc gtgatctcat ggagggtctc
     1861 gaggtgcacg tggtgcaggg cagcgggcac tgcccccacg aggagcgccc cgagctcacc
     1921 tcgaggctga tcgcagggtt cctgtgcgcg tgaggcgcaa tgataccatt cagggattca
     1981 gcgcttggat cccacccggg catccaggcg taccttcgtg cggccacgat gccacacggc
     2041 actcgggcga cccggcatgt ctgccccaag cccgcagatc aacgacacgg cctgcacgac
     2101 acggtctcgc ctcgaggctg acagctgggt tcctgtgcgc gcgaggcgcg ccgagacgcc
     2161 ctctgccctg ccgggtctca ccccttgctt gcaggcgcag gaaaggcggc accggcgcgt
     2221 tgaggcgccc ggcctggccg ccagcctcgt tgcatgcctg cataaggggc gacacgaaac
     2281 cgcatccaca tgggtggcag gctttccggc atgtcttccg cctcatgagg gcgtgtctgc
     2341 gagcggcacc ttcgggctag cccgcagcgt aacaccatgc agaccctctg cagcccccgt
     2401 gcgcaggcgc ctctcccgtg catgcatggt cctcaaaaaa gcgtcaccct ccccttccag
     2461 cgatcacgag ccgctctttt cccctcgatg caggaagcgg tgtggaaatg aaaggccacc
     2521 ccccgcgatg aaggcagtgt cccctttcat gatggtgtcg cggtcagcgg ggtgaggatg
     2581 gc
//
LOCUS       DAOSCR010000404         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26224_length_2571_cov_2.893879, whole genome shotgun sequence.
ACCESSION   DAOSCR010000404 DAOSCR010000000
VERSION     DAOSCR010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26224_length_2571_cov_2.893879"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLT69_09575"
     CDS             complement(<1..1040)
                     /locus_tag="PLT69_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ14728.1"
                     /translation="MPGIIAEPVYPRVIWFTAWQAAASTVLTLAAGLPGAYVYARYRF
                     FGKRLLYALTAVPFVLPSVVVASGFKALLGPRGVLNTWAMGAFHMEAPPIRIEHTIWF
                     ILLAHVFYNHAVVVRIVGGFWSRLPPSLAQAARTLGASQLKVFTGVTLPLLVPALAAA
                     SMLVFTFCFSSFGVILILGGPRFATIEVEIYRQAVHLFDLKTAGVLSLIQIGFALAFL
                     GFYTGLMRRTAHQFSPHPHELSQRPPSSAKDYALLFGNGMVVLALLVAPMAALAFQSV
                     HGPDGLTLDYYRRLFENTTDGLFIVSPLKALVSSVSFASATLVVSLVLGLATAIHLSR
                     DKGVFSRILDPVL"
     gene            complement(1171..2241)
                     /locus_tag="PLT69_09580"
     CDS             complement(1171..2241)
                     /locus_tag="PLT69_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ14729.1"
                     /translation="MDLRQRPARVLAATAVIVLCLALAAGCGTRREPASPTLTVMTHD
                     SFKIGDGVIKAFEQENSCKVVLLKAGDAGAALNQALLTKNNPMADVFYGVDNTFMGRA
                     LEADLFVPHPSPLLRDIPDDLELDKGYRLLPVDYGDVCLNYDKKWFMKGPLAPPASLE
                     DLLKPAYKDLLVVENPATSSPGLAFLLVTVGTFGEGGYLAFWKALKNNGVLVVSGWEE
                     AYWGQFSAASKGTRPIVVSYATSPAAEVYFSKEKLEAPPTEAVTAPGTTFRQIEFVGV
                     LKGAANPTLAARFVDYMLDRRFQEDIPLNMFVFPSNAKAALPEVFLRYARKAEKPVVI
                     DVHRLEKMREAWIRAWTDTVLR"
BASE COUNT          467 a          794 c          901 g          409 t
ORIGIN      
        1 agcacggggt ccagaatcct ggagaagacg cccttgtctc tcgagaggtg gatcgcggtg
       61 gcgagcccga ggacgagcga gacgacgagg gtggccgagg cgaaggacac cgacgagacg
      121 agggccttca gtggcgacac gatgaacagg ccgtctgtgg tgttttcgaa gagccgccgg
      181 tagtagtcga gggtgagacc gtcggggcca tggaccgact ggaaggcgag cgccgccatg
      241 ggcgcgacca ggagggcgag cacgaccatg ccgtttccga agagaagggc gtaatccttg
      301 gcggacgagg gcgggcgctg tgagagctcg tgggggtgag ggctgaactg gtgggcggtg
      361 cgccgcatga gccccgtgta gaaccccagg aaggcgagcg cgaagccgat ctggatcagc
      421 gagagtacgc ctgccgtctt caggtcgaag aggtggacgg cctggcggta gatctcgacc
      481 tcgatcgtcg cgaagcgggg cccgccgagg atgaggatga cgccgaagct cgagaagcag
      541 aaggtgaaca ccagcatcga cgccgctgcc agtgccggca cgaggagggg cagggtcacg
      601 ccggtgaaga ccttcagctg cgaagcgccg agggtcctgg cggcttgagc caggctgggc
      661 ggcagcctcg accagaatcc gccgactatc cgcaccacca ctgcgtgatt gtagaagacg
      721 tgggcgagga ggatgaacca gatcgtgtgc tcgatcctga tcggcggcgc ctccatgtga
      781 aaggcgccca tcgcccaggt gttcaggacc cccctgggcc cgagcaaggc cttgaaccct
      841 gaggcgacca cgaccgaggg cagcacgaag gggaccgcgg tcagcgcgta gaggaggcgc
      901 ttgccgaaga agcggtatct cgcatacacg taggcgccgg ggaggcccgc agccagcgtg
      961 agcacggtcg acgccgcggc ctgccacgcg gtgaaccaga tcacgcgagg atagaccggc
     1021 tcggcgatga tccccggcaa gccggccccg ccgccggggg gcgatacaag gctcacctcc
     1081 aggatacgcc agagggggta gaagaagaac acccccagga aggcgagagg gcctgccgct
     1141 atgaatgctc gcccgagtct cgcgatggcc ttacctcagc acggtatccg tccaggccct
     1201 gatccatgct tccctcatct tctccagcct gtgcacgtcg atgaccacgg gtttctcggc
     1261 cttcctcgcg taccttagga acacctcggg cagggcggcc ttcgcgttgg acgggaacac
     1321 gaacatgttg agggggatgt cttcctggaa gcgcctgtcg agcatgtagt ccacgaacct
     1381 ggccgcgagc gtagggttgg ccgctccctt gaggacgccc acgaactcga tctgcctgaa
     1441 ggtcgtgccg ggcgcggtca cggcctcggt cgggggggcc tcgagcttct ccttggagaa
     1501 atacacctct gcggcagggc tcgtggcata gctcaccacg atgggcctcg tgcccttgga
     1561 tgctgcgctg aactggcccc agtaggcctc ttcccaaccg gacacgacga ggacgccgtt
     1621 gttcttgagg gccttccaga atgcgaggta gccgccctct ccgaacgtgc ccacggtaac
     1681 caggaggaac gcgaggccgg gcgacgaggt ggcagggttc tccaccacga gcaggtcttt
     1741 gtaggcgggc ttcaagaggt cctcgaggga ggcgggcggg gcgagcgggc ctttcatgaa
     1801 ccacttcttg tcgtagttca ggcacacgtc cccgtagtcc accggcaaca gacggtaccc
     1861 cttgtcgagc tcgaggtcgt cggggatgtc cctgaggagg ggcgaaggat gcggcacgaa
     1921 gaggtccgct tcgagcgccc tccccatgaa cgtgttgtcc accccgtaga acacgtcggc
     1981 catggggttg ttcttggtga gcagggcctg gttgagggcg gcgccggcgt cgcccgcctt
     2041 caggagcacg accttgcacg agttctcctg ttcgaaggcc ttgatcaccc catcgcctat
     2101 cttgaagctg tcgtgggtca tcacggtcag ggtgggactg gcgggctctc tcctggtgcc
     2161 gcagcccgcg gcgagcgcga ggcagaggac gatcacggcc gtggcggcaa gaacacgcgc
     2221 cggtctttgt ctcaggtcca tcttcacctc catcgcaccc ttgcgcggcc cggccattcc
     2281 caagcggcag aggatcgtcg ggccgctcga gcagggctct ctcaacactt tgcgccaccg
     2341 ggccccggca gccgtgcatg cccggggata ccgccgcatg gccgcctatg cctcgggccg
     2401 atatctcgcc ggatgcacga gggcgcacag cttgactcga tcatgctatt gaggggatgc
     2461 cctcctgtca aggaccatct cccggggtct ctcgggggga ggaagaaggt gtgcggcgta
     2521 tcctccccgc ggacttcacc cacgatggca ggcctgcggg cagggtgtcc c
//
LOCUS       DAOSCR010000405         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26241_length_2570_cov_3.940755, whole genome shotgun sequence.
ACCESSION   DAOSCR010000405 DAOSCR010000000
VERSION     DAOSCR010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26241_length_2570_cov_3.940755"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLT69_09585"
     CDS             complement(<1..747)
                     /locus_tag="PLT69_09585"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ14730.1"
                     /translation="MKTRLATFDEKDLKEFEPENKVGLIATVNPEGLPHVTLITAIMA
                     KTPRVLVWGQFSEGLSKKHVLENPKTGFLIMTMDRSLWRGAARWTHKTGHGADYAAFN
                     AKPMFRYNAYTGIHTVHSMDLVWTWGRERLRLGRIVAASCACSLAYGAARSPQVERAM
                     NPWTKTLLGRMDTLTFLSYVGPDGYPLIVPLFACRAPDEGRLAFSPYAYEDELEAVEP
                     GAHVAVYGLSLQMESVLVRGVFRGYRRFRGV"
     gene            complement(744..1802)
                     /locus_tag="PLT69_09590"
     CDS             complement(744..1802)
                     /locus_tag="PLT69_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOJ14731.1"
                     /translation="MRNVTTTGTSAAGNSTQGLILRIQRMSTEDGPGIRSTVFFKGCP
                     LACRWCHNPESISSKVQVHWVASRCIGCLECVRTCRSHALSPSAGGMVIDRDRCTGCL
                     ACTEACPSTALEAYGVRYRLDDLVNEVLKDRVYFERSSGGVTLSGGEPTLQPEFALAL
                     LAKLKAEGVHTALDTCGQASWEVLSRLLPFTDIVLFDLKEMDPDRHKAFTGVTNSLIL
                     ENAQRVASSLRKNGRPSKMWVRTPLIPGHTARTDNVTELGSFIRRNLSGAVGRWDLCT
                     FNNLCLHKYQGLGIDWPLGGVELMGRAEADAMLEAARGSGVDPGIVRLSGPLRLETGR
                     DAPERPRAALGVVQGGKR"
     gene            complement(1832..>2570)
                     /locus_tag="PLT69_09595"
     CDS             complement(1832..>2570)
                     /locus_tag="PLT69_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOJ14732.1"
                     /translation="LLAVKYLVYDRKECTMDDLIQALKSNWEGYEALRPKARYKAPKY
                     GRDDDEADAMADRVMELWCEETWKHGTVSTKRRFRPGMLSWNYWVSDGFILPASPDGR
                     AKGTFLSNAICPSNGADINGPTANANSVGKALGGLCPDGSGDYLGYYNRLPNGASHTI
                     SFNPSILKDPEHKEKFKAFLRGYAHNGGTCLQVNMIDADTLRDAQEHPEDYRSLLVRI
                     TGYNAYFTSVGRELQDELIARLSHEAL"
BASE COUNT          399 a          864 c          809 g          498 t
ORIGIN      
        1 caccccccgg aagcgacgat acccgcggaa caccccgcgc acgagcacgc tctccatctg
       61 gaggctgagc ccgtacaccg cgacgtgggc gccgggctcc acggcctcga gttcgtcctc
      121 gtacgcgtac ggcgagaacg cgagcctgcc ttcatccggg gccctgcagg cgaaaagcgg
      181 cacgatgaga ggatacccat cgggccccac gtacgagagg aaggtgagcg tgtccatgcg
      241 gccgagcagc gtcttcgtcc aggggttcat cgccctttcc acctggggcg accttgccgc
      301 cccgtacgcg agagagcacg cgcacgatgc ggccacgatc cgccccagtc tcaggcgttc
      361 ccttccccat gtccacacga gatccatgga gtggaccgtg tgtatgcccg tgtacgcgtt
      421 gtacctgaac atgggcttgg cattgaaagc ggcgtagtcc gcgccgtgtc ccgtcttgtg
      481 cgtccacctg gccgcccccc tccagagcga ccggtccatg gtcatgatga gaaacccggt
      541 cttggggttc tcgagcacgt gcttcttgct caggccctcg gagaactggc cccacaccag
      601 cacacggggg gtctttgcca tgatcgccgt gatcaaggtg acgtggggaa ggccctcagg
      661 gttcacggtg gcgatgagcc ccaccttgtt ctcgggctcg aactccttga ggtccttctc
      721 gtcgaaggtg gcaagccgcg tcttcatctt ttccctccct gtacgacgcc gagcgcggcc
      781 cggggccttt ctggcgcatc ccgcccggtc tcgagcctca acgggccgct caggcggacg
      841 atcccggggt ccacccccga gcccctggcc gcctccagca tggcatcggc ctcggcgcgg
      901 cccatgagct cgacgcctcc caggggccag tcgatgccga gcccctggta cttgtgcagg
      961 cacaggttgt tgaaggtgca cagatcccac ctgcccaccg cacccgagag gtttcggcgg
     1021 atgaacgacc ccagctccgt cacgttgtcc gtacgggccg tgtggcctgg tatgaggggt
     1081 gtgcgcaccc acatctttga aggcctgccg ttctttcgca gcgatgaggc gaccctctgt
     1141 gcgttctcca ggatgagcga gttcgtcacg ccggtgaatg ccttgtgcct gtccggatcc
     1201 atctccttga ggtcgaagag cacgatgtcc gtgaaaggaa ggagccggct caacacctcc
     1261 caggatgcct gcccacaggt atcaagcgcc gtgtggacgc cctcggcctt gagttttgcg
     1321 agcaaggcaa gggcgaactc gggctgcagg gtgggctcgc cgcccgagag cgtaaccccg
     1381 cccgaagacc gctcgaagta taccctgtcc ttgaggacct cgttcacgag gtcgtcgagc
     1441 ctgtacctca cgccgtaggc ctcgagtgcg gtcgacgggc atgcctcggt acaggccagg
     1501 cacccggtgc acctgtccct gtcgatgacc atgccgcctg ccgacggtga gagggcgtga
     1561 gacctgcacg tcctgacgca ctccaggcac ccgatgcacc tcgaggccac ccagtgcacc
     1621 tgcaccttgc tcgagatgct ttccgggttg tggcaccacc tgcaggcgag cgggcacccc
     1681 ttgaagaaga ccgtgctcct gattcccggg ccgtcctcgg tcgacatgcg ctgtatgcgc
     1741 aggatcaggc cctgcgtgga gttccccgcc gcagacgtgc ctgttgtcgt gacgtttctc
     1801 atgggcatcg tcggtaccct tgccgccccc ctcacaacgc ctcgtggctc aacctcgcga
     1861 tgagctcgtc ctggagctcc cggccgacgc tcgtgaagta cgcgttgtac cccgttatcc
     1921 tcacgaggag ggaacggtag tcctccgggt gttcctgcgc gtctctcagg gtgtctgcgt
     1981 ctatcatgtt gacctggagg caggtcccgc cgttgtgcgc atacccgcgc aagaacgcct
     2041 tgaacttctc cttgtgctcg gggtctttga ggatcgatgg gttgaaggag atggtatggc
     2101 tcgcgccgtt cggaagcctg ttgtagtaac ccaggtagtc gccggacccg tccgggcaga
     2161 ggccgccgag ggcctttccc accgagttcg cgttcgccgt cggcccgttg atgtccgcgc
     2221 cgttcgatgg gcagatggcg ttcgacagga aggtgccctt ggccctgccg tcagggctcg
     2281 cgggcaggat gaagccgtcg gagacccagt agttccagct cagcatgccg ggcctgaagc
     2341 gccttttggt ggagacggtg ccgtgcttcc aggtctcctc gcaccacagc tccatgacgc
     2401 gatccgccat ggcgtccgcc tcgtcgtcgt ctcggccgta cttgggcgcc ttgtagcggg
     2461 ccttgggcct gagggcctcg tagccctccc agttggactt gagcgcctgt atgaggtcgt
     2521 ccatggtgca ctctttccgg tcgtagacga ggtatttgac ggccagcagg
//
LOCUS       DAOSCR010000406         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26243_length_2570_cov_3.467594, whole genome shotgun sequence.
ACCESSION   DAOSCR010000406 DAOSCR010000000
VERSION     DAOSCR010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26243_length_2570_cov_3.467594"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..650)
                     /locus_tag="PLT69_09600"
     CDS             complement(132..650)
                     /locus_tag="PLT69_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOJ14733.1"
                     /translation="MPEFYEMKVAGIALDPSNNAPIVILRDIDGTFILPIWIGIMEAS
                     AIAAALEGVVYSRPMTHDLFRSFVEHLGVVVEKIEVVDLKDNVFYALITVIKGDERLQ
                     IDARPSDAIAIALRTNSPIYVAEHVVKDAIRVDTAKPAAGKIMVGFEQDQEKLKEILE
                     KMNPEDFGKFKA"
     gene            complement(893..1750)
                     /locus_tag="PLT69_09605"
     CDS             complement(893..1750)
                     /locus_tag="PLT69_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ14734.1"
                     /translation="MVIECVDVSYRYQGSGPWAIDGVTLEVRPGERMVITGPAGSGKT
                     TLLQLMDALVLPVRGDVLFDGRSVRDLAARRELHAVRRRAGVLFQFPEDQFFHQSAYE
                     ELTFSLRNFFGSDEKEIHQRAASAASYLGLDTGRLKEVSPFSLSTGQKRRLALASALV
                     MEPEILMLDEPTAGLDPAGRKDVARVISSLGETAVVVVTHNLEDFLGVAERVVCLDAG
                     RVVFDASVEDLLARPDIARGYPFEIPLVVKAQQWLRAGGLELDGPSWDMDGLVRRLRL
                     LAGMPENGE"
     gene            complement(1741..>2570)
                     /locus_tag="PLT69_09610"
     CDS             complement(1741..>2570)
                     /locus_tag="PLT69_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ14735.1"
                     /translation="YGLLGMREIPPEAREGRAVEWDFFSSPQGSRLVDRADVKGVFHV
                     HTSASDGKASLQEMVMAAGARGYSWVGVSDHSRSAYYAGGLSVEGLEDQCRAIDALNE
                     GSAGITVLKGVESDILPDGSLDYPDEVLQRLDFVIASVHSHMDMDRAAMTRRVLRALE
                     NPRTSVLGHPTGRLLLSRRPYELDVDAVLDAASRLGVIVELNAHPMRLDLDWRLIRDF
                     TGAGGLVAVNPDAHEPSGYDDMRYGLVMARKGLLSAASCVNCMDVREVREVVSRRWS"
BASE COUNT          444 a          894 c          795 g          437 t
ORIGIN      
        1 ttccaccatg gcctggaaga cggccttggt gaggcttttt gtccttgagc gtcgagagag
       61 cggtgcccgc gcattcacgc tgggaacata cacgatgggg gtgcgtgaat caaggttcat
      121 cccttacggc ttcaggcctt gaacttgccg aagtcctcgg ggttcatctt ctcaaggatc
      181 tccttgagct tctcctggtc ctgctcgaag ccgaccatga tcttgccggc ggcgggcttg
      241 gccgtatcca cgcggatggc gtccttgacc acgtgttcgg ccacgtagat gggggagttc
      301 gtcctgaggg cgatcgctat ggcgtcagag ggcctcgcgt ctatctggag acgttcatcc
      361 cccttgatca cggtgatcag ggcgtagaat acgttgtcct tcaagtccac gacctcgatc
      421 ttctccacga cgacgcccag gtgctcgacg aaggaccgga agaggtcatg ggtcatgggc
      481 ctggagtaga ccacaccctc gagggccgcc gcaatggcgc tcgcctccat gatccctatc
      541 cagatgggca ggatgaatgt gccgtcaatg tccctcagga tgacaatggg ggcattgttg
      601 ctcgggtcga gagcgatacc ggccaccttc atctcgtaga attccggcat gtgcacccac
      661 ctcgatacat aacattaagc ggcttgcccc agagagtcaa gtgatacgaa acccggcgga
      721 aagccccggg gacgagcccg tgcatcgacc ggtcgcgacg cttcccggca cacgcgttga
      781 gcccgccccg agggcgcagg gcggccgagg gcgatgcgca ccgcggcctc gggaggctga
      841 cctgggacga cgcccgtgcc gccttcaact ggcccgaacg ccgcgggagg cctcactccc
      901 cgttttcggg catgcctgcc agcagcctca ggcgacggac gaggccgtcc atgtcccagg
      961 acggcccgtc cagctcaagt ccaccggcgc ggagccactg ctgcgccttc accacgaggg
     1021 ggatctcgaa cgggtacccc cgggcaatgt ccggcctcgc gaggagatcc tccaccgatg
     1081 cgtcgaagac gaccctgccg gcgtccaggc acacgaccct ctcggccacg ccgaggaaat
     1141 cctccaggtt gtgggtgacc accaccacgg ccgtctctcc cagcgacgag atgaccctcg
     1201 cgacgtcctt cctcccagcc gggtcgagac cggcggtcgg ctcgtcgagc atcaggatct
     1261 cgggctccat gaccagggcg ctcgccaggg cgagcctccg cttctggccc gtgctcagcg
     1321 agaaggggga gacctccttg aggcggcccg tatcgagccc caggtacgag gcggccgagg
     1381 ccgccctctg gtgaatctcc ttctcgtccg atccgaagaa gttgcgcaac gagaaggtga
     1441 gttcctcgta ggcgctctgg tgaaagaact ggtcctcggg gaactggaag agcaccccgg
     1501 cccgcctgcg cacggcgtgg agttcccgcc gtgcggccag gtcacgtacg ctcctgccgt
     1561 cgaagaggac gtcgcccctt acgggcagca cgagtgcgtc catgagctgg agcagggtcg
     1621 tcttcccgga gcccgcaggc cccgtgatca ccatgcgctc accgggccgc acctccaggg
     1681 tcacgccgtc tatcgcccac ggccccgacc cttggtagcg atacgataca tccacgcact
     1741 ctatgaccat cgcctggata ccacctctct cacctcgcgc acgtccatgc agttgacgca
     1801 ggaggccgcg gacaacagcc ccttgcgggc catcacgagg ccgtaccgca tgtcgtcgta
     1861 gcccgagggc tcgtgggcgt ccgggttcac cgccaccagc cccccggccc cggtgaaatc
     1921 cctgatcagc ctccagtcga ggtcgagcct catggggtgc gcgttcagtt ccacgatcac
     1981 cccgagcctg gaggcggcat ccaagacggc gtccacgtcc agctcgtagg gcctcctgga
     2041 caagagcagc ctgcctgtcg ggtggccgag cacgctcgtg cgcgggttct ccaaggcccg
     2101 aaggacccgt ctcgtcatgg cggccctgtc catgtccatg tgcgagtgca cgctcgcgat
     2161 gacaaagtcg agccgctgga gcacctcgtc ggggtaatca agggagccgt ccggcaggat
     2221 gtcggactcc acccccttga gcacggtgat gcccgccgaa ccctcgttca aggcgtcgat
     2281 ggcccggcac tggtcctcca ggccctccac ggagagcccc cccgcatagt aggcgctcct
     2341 gctgtgatcc gagacgccga cccacgagta gcccctcgct cctgcggcca tgaccatctc
     2401 ctgaagcgac gccttgccgt cgctcgcgga cgtgtgcacg tggaagacgc ccttgacgtc
     2461 cgcgcggtcc acgagcctcg agccttgggg ggacgaaaag aaatcccact ccaccgccct
     2521 gccctcacgg gcctccgggg ggatttcccg catgcccaac aggccgtaga
//
LOCUS       DAOSCR010000407         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26255_length_2569_cov_4.393397, whole genome shotgun sequence.
ACCESSION   DAOSCR010000407 DAOSCR010000000
VERSION     DAOSCR010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26255_length_2569_cov_4.393397"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..481
                     /locus_tag="PLT69_09615"
     CDS             164..481
                     /locus_tag="PLT69_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14736.1"
                     /translation="MKGSTKDLLQIQGVQGFVLTDGRNVQVKLPSTHPLSGAKERFKR
                     LHQELGRKGARPGNTVEILLDNVLITLFFSGSTMLAVLASPDVNQALLRITGRLIIAG
                     SPG"
     gene            495..1310
                     /locus_tag="PLT69_09620"
     CDS             495..1310
                     /locus_tag="PLT69_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOJ14737.1"
                     /translation="MNKDIGGDISNTRVLEIVKILSLGKRTGRLHVTNGAETCNIFFS
                     EGQVVHAHCSGLTGIKAIQEASAWTSGEYRFFVDEQADMQSVFMGTDDVLLEVTNHLR
                     QMDKVTSLIPSPAMVYALETDIRESEVQIKAAQWKVLSLVDGRKSIADIAQLVGIASS
                     DAMKIFYTLLRSGLIREVSSEERDTGVGPTLNLPKTAFIESLVAALTRAIGPIAPYVV
                     QETARDITADLLSEDPAQRAFLVETIADKIPEGRMSLAFLESMAEKLKEERAV"
     gene            1310..2062
                     /locus_tag="PLT69_09625"
     CDS             1310..2062
                     /locus_tag="PLT69_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14738.1"
                     /translation="MRTKAFVIVLSSLCLMALVSSAILFLVGLEIRGVNLGTLSDSIS
                     KEDARILSLILEHIPPEKIEEMALPESWEEIFILDPSELTVVSSTTPGHRGMPLYRHP
                     LLLDHAKVVVSAIKAQKTTIEETPSCRVVAAPLPDGRLTVAIKPKAWEQGLVQRQEEE
                     VRRTTSSIWIVAAAFTILGILVSVFAAFLVARKVTATTDKAVTALEALSLGDFSVDLD
                     ALGSAGELDRFKDSFVRLKTSLTMALEMLSRR"
     gene            2064..>2569
                     /locus_tag="PLT69_09630"
     CDS             2064..>2569
                     /locus_tag="PLT69_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ14739.1"
                     /translation="MPKQVLICDDEETLTWSLARSLSHDREIYEVTTVNDGDTALSEL
                     ERHPYDVVVLDIRMPGMNGLDVLLHIKEKTPATKVIMMTAYGSQEIKEKARARGSMYY
                     LEKPFEIEQLRGLILKALKEDSSRGFAGSVSGLHLADLIQMNCLSQMTTALFVEKGDR
                     KGCIYFEDG"
BASE COUNT          532 a          797 c          786 g          454 t
ORIGIN      
        1 aaaagagcct tctcaagcct ttcttgatct ttttgaacat ggtgccgaaa accgccttgt
       61 cgttttctct cgataatgat cttatcggtc agggggcgcc gcgcttgagg atcatcgagc
      121 ctcgtaaagc ctcgcgcaca aggtgccgat aggttgaacg attatgaagg gatcgaccaa
      181 ggacctcctc cagattcaag gcgtccaggg cttcgtgctg acagacggca ggaacgtgca
      241 ggtgaaactc ccgtccacgc accccctgtc aggcgcgaag gagcggttca agcgcctgca
      301 ccaggagctc gggcgcaagg gcgccaggcc cggcaacacg gtggagatcc tcctggacaa
      361 cgtgctcatc acgctctttt tctccggctc cacgatgctc gccgtgctgg cgagccccga
      421 cgtgaaccag gccttgctca ggatcaccgg caggctcata atagcaggca gtccaggatg
      481 acggggtcat cgagatgaac aaggacatcg gcggggacat ctccaacacc agggtcttgg
      541 agatcgtgaa gatcctctcc ctggggaagc gcaccggcag gctgcacgtg acgaacggcg
      601 ccgagacgtg caacatcttc ttctcggaag gccaggtcgt tcacgcgcac tgttccggcc
      661 tgaccgggat caaggccatc caggaggcct cggcgtggac ttccggcgag taccgcttct
      721 tcgtcgacga gcaggcggac atgcagagcg tcttcatggg cacggacgac gtcctcctgg
      781 aagtgaccaa ccacctgcgc cagatggaca aggtcacgtc tctcatcccg tcgcccgcca
      841 tggtgtatgc actggagacg gacatcaggg agagcgaggt gcagatcaaa gcggcccagt
      901 ggaaggtgct ctccctcgtc gacggcagaa aatcgatcgc ggacatcgcc cagctcgtgg
      961 ggatagcgtc ctcggacgcc atgaagatct tctacacact cctgaggtcc ggcctcatac
     1021 gggaagtgtc cagcgaggag agggacaccg gtgtcggccc caccctgaac ctcccgaaga
     1081 ccgccttcat cgagtcgctc gtggccgcgc tcacgagggc cataggcccc atcgccccgt
     1141 acgtggtgca ggagaccgcg agggatatca cggccgacct cctgtccgag gacccggccc
     1201 aacgggcgtt cctcgttgag accatagccg acaagatacc cgagggcagg atgtcgctgg
     1261 cattcctcga gtcgatggcc gagaagctca aggaagaaag ggcggtctga tgaggacaaa
     1321 ggccttcgtg atcgtcttgt cttccctgtg cctcatggcg ctcgtgtcgt cggcgatcct
     1381 ctttctcgtg ggtctcgaga tccgtggggt gaacctgggc acgctgtccg actccatctc
     1441 gaaggaggat gccaggatcc tgtcgttgat cctggaacac ataccgcccg agaagatcga
     1501 ggagatggcc ctccccgaat cgtgggaaga gatcttcatc ctcgatccat ccgagctcac
     1561 cgtggtctcc tccacgacgc ccggacacag gggcatgccc ctgtacaggc accccctgct
     1621 cctcgaccat gccaaggtgg tggtctcggc gatcaaggcc cagaagacaa ccatagaaga
     1681 gacgccgtcc tgccgggtcg tcgcggcccc gcttcctgac ggccgactca cggtggcgat
     1741 caagccgaag gcgtgggagc agggccttgt ccaacgccag gaagaggagg tgaggaggac
     1801 gacctcgagc atatggatcg tcgcggccgc gttcacaatc ctggggatcc tcgtctccgt
     1861 cttcgcagct ttcctggtgg ccaggaaggt gaccgccacg accgacaagg ccgtcacggc
     1921 gctcgaggcc ctcagcctgg gcgacttcag cgtcgacctc gacgcgctgg ggtcggccgg
     1981 agagctggac cgcttcaagg actcgttcgt gaggctgaag acatccctga ccatggcact
     2041 ggaaatgctt tcccggagat gacatgccca agcaggtcct catctgcgac gacgaagaga
     2101 cgttgacctg gtctcttgca aggtccctct cgcacgacag ggagatctac gaggtgacca
     2161 ccgtcaacga cggcgacaca gccctttcgg agctcgagag gcacccctac gacgtcgtgg
     2221 tcctggatat caggatgccg ggcatgaacg ggcttgacgt cctgctccac atcaaggaaa
     2281 agacccctgc caccaaggtc atcatgatga ccgcctacgg gtcccaggag atcaaggaaa
     2341 aggcgagggc caggggctcg atgtactacc tggagaagcc cttcgagatc gagcagttga
     2401 gggggctcat cctcaaggcc ctcaaggaag actcctccag ggggttcgcc ggcagcgttt
     2461 ccggcctgca cctggcggat ctcatccaga tgaactgcct cagccagatg accaccgctc
     2521 tcttcgtcga gaagggggac cgcaaagggt gcatatactt cgaggacgg
//
LOCUS       DAOSCR010000408         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26413_length_2558_cov_2.534559, whole genome shotgun sequence.
ACCESSION   DAOSCR010000408 DAOSCR010000000
VERSION     DAOSCR010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26413_length_2558_cov_2.534559"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(496..2220)
                     /locus_tag="PLT69_09635"
     CDS             complement(496..2220)
                     /locus_tag="PLT69_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ14740.1"
                     /translation="MSEYAEPWLKRYEEETRELYYQRVLAILMIGIVLIPLFSVLDYM
                     VVRQYAREFLVYRLGCAAVLSVFLAVYFSRWGKAWARIVSVIASIVSALTITLMCVRM
                     GGYDSFYYVGVVMVLVAVLAILPLEALQATVLGVVLYAVYAVPVIVFSDATPQRLMIF
                     FSNSFFFAFFIVISVLKCYEDTRVRKRETQLKVEMDGLMERLSYYAHNLEAEVDKRMK
                     QLEESELRYRELYENIIDIVLLVGKNAKVLMANPKFYEVTGHRGGDAGARSVYDYIHP
                     KDVSVVERMIIRLPVEQAVKDLQFRLRGATGKIVDVECNAKCITKNSQVVGFQLVLRD
                     ITVRKKLEHDLLESYKKVQSARNATILGLAKLAEYRDSDTGAHLERIREYCKVLAEEL
                     ATKPAYEGYITPEYIEDIYNSSILHDIGKVGIPDSILLKPGKLTPEEFEVVKRHSALG
                     GEALKEVEAKIEGQSFLTLGKEIAYYHHEKWDGSGYPKGLKGEQIPLSARIVALADVY
                     DALTSRRVYKEAYSHEEAKRIIVEGRGTHFAPDVVDAFLANEHLFRKIREEMFADRPG
                     RDKVNTAA"
BASE COUNT          446 a          834 c          795 g          483 t
ORIGIN      
        1 cgcatacgac gaccgtatcc agggtgggcc atcccttccg ggtatacggg gcggggggtg
       61 gaggggttcg acgggcacct cctgaaccca ctccggcaag atccatcccc tccaggacgg
      121 gcacaggctc aagaggccgc cgaggggctg tcaggcgggt atcgcgaagg gcatcccggc
      181 cttccctgtc tcgtccacac gcataccgtg agaacacgcc ccggggtgga catgcaaccc
      241 ggcttcgcat cgtccttgaa cgagacactc ccccgctctt gaacgggggg ctctgcgcac
      301 gcccgccccg ggaagccgcc gtcgcgggca tgtccgggca agggtgacat ggcgcatgtc
      361 gggcgtgatg tgtcttgcgc atgggagaaa gggccttctt ccgggggatg tacgccattg
      421 cgcctgtcag gccgaggcag tccgggtccg atgatctccg gccgggcgtt caggcgggaa
      481 agagccccgg cggcctcagg cagcggtgtt gaccttgtcc ctgccgggcc ggtcggcgaa
      541 catctcctcc ctgatcttcc tgaagaggtg ctcgttcgcc aggaacgcgt ccaccacgtc
      601 gggagcgaag tgcgtccccc tgccctccac gatgatcctc ttcgcctcct cgtgggagta
      661 ggcctccttg tacacccgcc tggacgtgag ggcgtcgtac acgtccgcaa gcgccacgat
      721 ccgggccgag agggggatct gttccccctt gaggcccttg gggtagccgg aaccgtccca
      781 cttctcgtgg tggtagtagg cgatctcctt gcccagggtg aggaaggact ggccctcgat
      841 cttcgcttcc acctccttga gggcctcgcc gcccagcgcg gagtggcgct tgaccacctc
      901 gaactcctcg ggcgtgagtt tccccggctt caggaggatc gaatccggga tgcccacctt
      961 ccctatatcg tggaggatcg aggagttgta gatgtcctcg atgtactcgg gcgttatgta
     1021 gccttcgtag gccggcttgg tggcgagctc ctcggccagg accttgcagt actcgcgtat
     1081 cctctccagg tgcgcgccgg tgtccgagtc cctgtactcg gcgagcttcg cgagcccgag
     1141 gatggtcgcg ttcctcgcgc tctggacctt cttgtacgac tcgagcaggt cgtgctccag
     1201 cttcttcctc accgtgatgt ccctgagcac gagctggaag cccaccacct gggagttctt
     1261 cgtgatgcac ttcgcgttgc actccacgtc cactatcttg ccggtggccc ccctgaggcg
     1321 gaactgcagg tccttgaccg cctgctccac cggaaggcgt atgatcatgc gctccacgac
     1381 gctcacgtct ttcgggtgga tatagtcata caccgacctg gcaccggcat ccccccctct
     1441 atggcccgtg acctcgtaga acttggggtt ggccatgaga accttcgcgt tcttgcccac
     1501 gaggagcacg atgtcgatga tgttctcgta gagctcgcgg tacctgagct cagactcctc
     1561 gagctgcttc atgcgcttgt ccacctcggc ctcgaggttg tgcgcatagt acgacagcct
     1621 ttccatgagc ccgtccatct ccaccttgag ctgggtctcg cgcttcctga ccctcgtgtc
     1681 ctcgtagcac ttgagcaccg atatcacgat gaagaaggcg aagaagaagc tgttggagaa
     1741 gaagatcatg agcctctgtg gggtcgcatc ggaaaagacg atcaccggaa ccgcatacac
     1801 cgcgtacaac acgacgccga gaacggtcgc ctggagggct tccaggggga ggatcgcgag
     1861 caccgcgacg agcaccatca ccacccccac gtagtagaag gagtcgtacc ctcccatcct
     1921 gacgcacatg agggtgatgg tcagcgcgga gacgatcgag gcgatcacgg agacgatccg
     1981 ggcccaggcc tttccccacc tcgagaagta caccgccagg aacacgctca ggaccgcggc
     2041 gcaccccagc ctgtagacga ggaactcccg ggcgtactgc ctgacgacca tgtagtcgag
     2101 tacgctgaag agcgggatca gtacgatccc gatcatgagg atggccagca cgcgctggta
     2161 gtagagctcg cgcgtctcct cctcatagcg cttgagccat ggttctgcgt attcggacat
     2221 ctcccctcct ctatcggcac attcacgaag aaaactgaag gtcaagatct ctgtcagaag
     2281 gtctctccag ggagtgcggc atccgtgcga cccgtgggcc gggagggtca cgccccggga
     2341 aacgcatgcg gggcgtttct tcgtgaaggc gtccaaggtg ggcatgcctg aggcggcggg
     2401 tcaccggcca tgcatgaggg ctcaccgagg cgaccgaagg ccgcctcacc ccaagaccgc
     2461 catccagggg gagctgcctt cgggtcatgg agcggacccg tccctgagcg gttcgtgtct
     2521 caggggcccc gcctcccagg ggctaaagca cgcgttcc
//
LOCUS       DAOSCR010000409         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26423_length_2557_cov_3.485612, whole genome shotgun sequence.
ACCESSION   DAOSCR010000409 DAOSCR010000000
VERSION     DAOSCR010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26423_length_2557_cov_3.485612"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1457)
                     /locus_tag="PLT69_09640"
     CDS             complement(72..1457)
                     /locus_tag="PLT69_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOJ14741.1"
                     /translation="MAIRDLIRKAPKPGSLKSPPFAFILSYLLAVMVGGVLLKSPACL
                     EEGRHISWIDAFYTATSAVCVTGLTVVETGRTFSTAGELVILALIQFGGFGLMAFFAM
                     AFLWAGSRVSTQQSSFIKENYSQTMIYDARLILRILLAYTLLCEAVGVILLVPAWDHP
                     MTTWQRLYFGLFHSVSAFNNAGFSLFAENLCGYRGDILLNLTITSLVILGGIGSPVIL
                     DCWSYLHSPTRFRFSLHTKLAVSTTAALIAGGTAAIWLIQRPSPLFEIPLHEQALISY
                     FQAVTARTAGFNTIDLRQLGTAPIMIIILLMFVGASPGSCGGGIKTTSLATLLVVLWN
                     RIKGRSVNNVFRTTLPTKVVNRTISIFILASIFVIVVLTALLITQVGELPHDKSGGMF
                     IEYLFETISAFGTVGLSLGATAKMDGVGKILIMLTMLVGRVGIMTIAYLFTSRESTAR
                     YQFVEEDVMIG"
     gene            complement(1846..>2557)
                     /locus_tag="PLT69_09645"
     CDS             complement(1846..>2557)
                     /locus_tag="PLT69_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ14742.1"
                     /translation="ALSNDIRGVFWIATAPAVLAVALLALGVRERNPGRREHEPVALR
                     VSTLRRLPGTFWVVTALGAVFTLARFSEAFLLLRAEDMGVGRSLIPLVLVLMNVVYAV
                     SAYPAGVISDTVGRIGMVVVGGLLLAAADVALAFAQSPGILLCGVALWGLHMGLTQGV
                     FSTLVAETCPTDLRGSAFGVFGLACGIAVLGASTLAGWLWDFRGPSAAFLAGACIVVF
                     ALVGFSAFGGYARKGRMP"
BASE COUNT          492 a          807 c          867 g          391 t
ORIGIN      
        1 cgacccagaa cccgaagttg cccagtccaa tgaccacgta ctttctcttc atggatacgg
       61 tcctttcacg gtcatcctat catcacgtct tcctcgacga actggtagcg cgcggtggac
      121 tccctgctcg tgaagaggta cgcgatggtc atgatcccca ccctgccgac gagcatggtg
      181 agcatgatga ggatctttcc gactccgtcc atcttcgccg tcgccccaag ggacagcccg
      241 acggtgccga aggccgatat ggtctcgaag aggtactcga tgaacatccc gccgctcttg
      301 tcgtgcggga gctcgcccac ctgggtgatg agcagggcgg tcaggacgac gatcacgaag
      361 atcgaggcga ggatgaagat ggagatggtc ctgttcacca ccttggtcgg gagcgtcgtc
      421 ctgaacacgt tgttgacgct tcgccccttt atcctgttcc agagcaccac gagcagggtg
      481 gccaggctgg tcgtcttgat cccgccgccg caggaccccg gcgacgcacc cacgaacatg
      541 aggagaatga tgatcatgat gggggcggtg ccgagctggc gcaggtctat ggtgttgaac
      601 cccgcggtac gcgcggtcac cgcctggaag tacgagatga gcgcctgttc gtgcaggggg
      661 atctcgaaga gcggcgaagg cctctgtatg agccatatgg ccgccgtgcc ccctgctatg
      721 agcgcggcgg tggtgctcac ggcgagcttg gtgtggagcg agaagcggaa cctcgtgggc
      781 gagtggaggt acgaccagca gtcgagtatc accggggagc cgatcccgcc taggatgacg
      841 agcgaggtga tcgtgaggtt gagcaggatg tcgcccctgt agccgcagag gttctcggca
      901 aagagggaga accccgcatt gttgaaggcg gagacggaat ggaacaggcc gaaatagagc
      961 cgttgccatg tcgtcatggg gtggtcccaa gccggcacga ggagaatcac tcccaccgcc
     1021 tcgcacagga gggtgtaggc caggaggatc ctcaggatga gccgtgcgtc gtagatcatg
     1081 gtctgggagt agttttcctt gatgaacgac gactgctgcg tgctcaccct cgagccggcc
     1141 cacaagaagg ccatggcgaa gaaggccatg agaccgaacc ccccgaactg gatgagcgcg
     1201 aggatgacga gttctccggc cgtggaaaag gtccggccgg tttccaccac ggtcagcccg
     1261 gtcacacaga cagccgaggt ggccgtgtag aaggcgtcga tccacgagat gtgccggccc
     1321 tcctcgaggc aggccgggct cttgaggaga acgccgccga ccatgacggc cagcaggtag
     1381 ctcagtatga aggcgaacgg tgggctcttc agagagcccg gcttcggggc ctttcggatg
     1441 aggtcgcgta tcgccataga gcctcgcccg cactccctcg cgggtcgcgg gtgacgccag
     1501 ttgccatagc acacgtggtt cgcgcgaatc aagtcgtcgc gatcgtcccg ggtgcaggac
     1561 gggcaggctc gtctgtaccg ggaaagacag gccagagagg gtgtccgcgt aacggggatc
     1621 caccgccgcg atcacggtct tcccaggggc tgtcatcgcc gaggccgtcg tgcgtgcggg
     1681 ggcgtggccc actgccgcgc tcacgggtcg gacaagcacg gaggagactc tccacacatg
     1741 cgccgctttg gcgcgcgcaa tgcagccgga aggcgcgggt gtccgaatcc tcgaggcgct
     1801 gttcaccccg aggcgggcgg acacccggcc cggagagaat cgccctcagg gcatgcggcc
     1861 cttcctcgcg tatccgccga acgccgagaa gccgaccagc gcgaagacga cgatgcatgc
     1921 cccggccagg aaggcggcgg acggtcccct gaagtcccac agccagccgg cgagcgtgct
     1981 cgcaccgagc acggcgatgc cgcacgccag gccgaagacg ccgaaggcgc tccccctgag
     2041 gtcggtcggg cacgtctctg ccacgagcgt ggagaagacc ccctgggtga gccccatgtg
     2101 cagaccccac agcgcgaccc cgcacaggag gatacccggg gattgggcga aggcgagcgc
     2161 gacgtcggcg gcggccagca agaggccgcc tacgacgacc atgccgatcc ttcccacggt
     2221 gtccgagatg acgcccgccg ggtaggcgct caccgcgtag accacgttca tgagcacgag
     2281 gaccaggggg atgagagacc gccccacgcc catgtcctcg gccctgagaa gcaggaaggc
     2341 ctcgctgaag cgggcgagcg tgaagacggc gcccaaggcg gtcacgaccc agaaggtccc
     2401 ggggagccgc ctgagggtcg ataccctcaa cgcaacgggc tcgtgttccc tcctgccggg
     2461 gttgcgctcc ctcaccccga gtgcgagcag ggccaccgcc agcactgcag gcgccgtcgc
     2521 gatccagaac acgcccctga tgtcgttcga cagcgcg
//
LOCUS       DAOSCR010000410         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26430_length_2556_cov_3.536985, whole genome shotgun sequence.
ACCESSION   DAOSCR010000410 DAOSCR010000000
VERSION     DAOSCR010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26430_length_2556_cov_3.536985"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..524)
                     /locus_tag="PLT69_09650"
     CDS             complement(207..524)
                     /locus_tag="PLT69_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14743.1"
                     /translation="MIRTDSNAVLSVAHHHCCGLDVHKDKASAAAITAHEDKMIEGSI
                     CELGNFSEGQYRLKDLVCEQGCMWWRMTAWNAWCSSDDATFPSMAGYDRKDSITFFPI
                     SLG"
     gene            677..1774
                     /locus_tag="PLT69_09655"
     CDS             677..1774
                     /locus_tag="PLT69_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOJ14744.1"
                     /translation="MEANGLKSNGSRGSGKRVFSAAIVVLAIGLLVFLYVRYTRLNVS
                     TDDAFVAGRIHTVAPRVQGTVKKVFVEDNQPVNEGDLLVEIDPRDYEVRVAEAEAAFE
                     AERSKLAEYTARLDAARAQIDESKARIETARATLELQQASRRQAYSDLERARRLFEQG
                     VMAQASYEKAQTAYDVAKAQESAASQSLRQAQASLATQEKAVRQAEDAVATQRRVVEQ
                     KLKASEAARLLLSYTSVRSPARGYVTRKNVEAGNTVAAGQPLMSVVSLEDVWVIANYK
                     ETQLANVRPGQEVTVKVDTYPGRTFDGRVHSIMAGTGSVFSLFPPENATGNYVKVVQR
                     IPVKIVLDTGQDPDHVLRVGMSVRPTIHIKR"
     gene            1789..>2556
                     /locus_tag="PLT69_09660"
     CDS             1789..>2556
                     /locus_tag="PLT69_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOJ14745.1"
                     /translation="MVDHPPLRPNKWLVAATVMLPMLMVVLDTSVVNVSLGHIRGSLS
                     AGIDEATWSITAYLAANAVIIPMAGWLSRLVGRKRYLIFSIVTFTISSFLCGASWNIE
                     SLIVFRILQGLSGGAMPPLSQAILLESFPPNQYGMAMAIFGIGVSFGPIIGPVLGGWI
                     TEALSWHWVFFINIPVGVLSTVMALFVVVDPPYMRGTLMKIDYAGLFLLAVGVGSLQL
                     VLDRGQRYDWFGSRLILALTIVSVVSLAAFVMVERRAS"
BASE COUNT          488 a          764 c          815 g          489 t
ORIGIN      
        1 atgaccgagg aatggctcgg ccgccgatac ctcatcttcg aagatgaggt atcgggcaag
       61 aagacggagg atctactggc ggcatgatgc ctacatcgga gatgccaatg tacagaaaat
      121 gggggacttg actggcattc ttccctgggc acagagaaga cctgcatttc atggattgag
      181 agattcctcc tatttcatca catgcgtcat cccagggaaa tgggaaagaa ggtgattgag
      241 tctttcctgt catacccggc catcgaaggg aaggttgcat catcactaga acaccaggca
      301 ttccaagccg tcatgcgcca ccacatgcac ccctgctcac aaaccaggtc cttcagccgg
      361 tactggccct cggaaaagtt cccgagctcg cagatgctcc cttcgatcat tttgtcttca
      421 tgggccgtga tcgctgccgc cgatgccttg tccttgtgaa catcaagacc gcaacaatga
      481 tggtgcgcca cgctcaaaac tgcgttagaa tctgtgcgga tcatgccctg catcctcgat
      541 ggtcttggtg agacagaacc atcggtgggc aattcatggg ccttctgcta cactatgttc
      601 gtcattcgtt atgcccgctc gcgggcatgg gggtattacg gtataacaac atgacaatgc
      661 cgggtgaggg gttggtgtgg aagcgaacgg gttgaaatcg aatggctcaa ggggttcggg
      721 caagagggtg ttttccgctg cgatagtggt ccttgcgatc ggtctccttg tctttctcta
      781 tgtcaggtac accaggctga acgtgtcgac cgacgatgcc tttgtcgcgg gtcgtatcca
      841 cacggtggca cccagggtcc agggtaccgt caagaaggtc ttcgtggagg acaaccagcc
      901 cgtgaacgag ggtgacctcc tcgtggagat cgaccccagg gactacgagg tccgcgtggc
      961 agaggccgag gccgcgttcg aggcggaacg ctcgaagctc gccgagtaca cggccaggct
     1021 ggatgcggcg cgtgcgcaga tagacgagtc caaggcccgt atcgagacgg cgcgtgccac
     1081 cctcgagctc cagcaggcat cgaggagaca ggcgtattca gacctcgaac gggcgaggag
     1141 gctcttcgag cagggcgtga tggcgcaggc ctcctacgag aaggcccaga cggcctacga
     1201 cgtagccaag gcgcaggagt cggccgcatc ccagtctctc aggcaggccc aggcatcgct
     1261 tgcgacgcag gaaaaggccg tccggcaggc cgaggacgcc gtcgcgaccc aacgccgggt
     1321 tgtcgaacag aagctgaagg cctcggaagc ggcgaggctc ctcctctcct acacgtcggt
     1381 ccggtcgccg gcgcgcgggt acgtgaccag gaagaacgtg gaggcaggca acaccgttgc
     1441 agcaggccag ccactcatgt ccgtcgtgtc gctcgaggac gtctgggtca tagcgaacta
     1501 caaggagacc cagctcgcga acgtgcgccc cgggcaggag gtgaccgtga aggtggacac
     1561 ctacccgggc aggacgttcg acggcagggt gcacagcatc atggccggca cgggttccgt
     1621 gttctccctg ttcccgcccg agaacgcgac gggcaactac gtgaaggtcg tgcagcgcat
     1681 ccccgtcaag atcgtgctcg atacaggcca ggaccccgac cacgtgctga gggtcggcat
     1741 gtccgtgagg ccgacgatcc acatcaagag atagggcggg gcctgaaggt ggtcgatcac
     1801 cccccgctgc gcccgaacaa gtggctcgtc gcggccacgg tcatgctccc catgctcatg
     1861 gtggtgctcg acacctcggt cgtgaacgtc tccctgggac acataagggg cagcctctcg
     1921 gcaggcatag acgaggcgac gtggtccatc accgcgtacc tggcggcgaa cgcggtgatc
     1981 atcccgatgg cggggtggct gagcaggctc gtgggcagga agcgctacct catcttttcc
     2041 atcgtcacct tcacgatcag ttcttttctg tgcggggcct cgtggaacat cgagagcctc
     2101 atcgtgttca ggatcctcca gggcctctcg ggcggcgcca tgccgccctt gtcccaggcc
     2161 atcctcctcg agtcgttccc gcccaaccag tacgggatgg cgatggccat cttcgggatc
     2221 ggcgtgagct tcgggccgat catcgggccc gtcctgggcg ggtggatcac cgaggcgctc
     2281 agctggcact gggtcttctt catcaacatc cccgtgggtg tcctctccac ggtgatggcg
     2341 ctcttcgtcg tggtcgaccc cccgtacatg aggggaaccc tgatgaagat cgactatgcc
     2401 ggcctcttcc tgctggccgt gggcgtgggg tcgctccagc tggtcctgga cagggggcag
     2461 cggtacgact ggttcggatc gcgcctcatc ctcgcgctga ctatcgtctc cgtcgtgtcg
     2521 ctggcggcgt tcgtgatggt ggagaggcgt gcgagc
//
LOCUS       DAOSCR010000411         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26459_length_2554_cov_3.470188, whole genome shotgun sequence.
ACCESSION   DAOSCR010000411 DAOSCR010000000
VERSION     DAOSCR010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26459_length_2554_cov_3.470188"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..430)
                     /locus_tag="PLT69_09665"
     CDS             complement(32..430)
                     /locus_tag="PLT69_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOJ14746.1"
                     /translation="MGFDTLYLRDDDPHRLLGAALSGRIVLTRRRALRGRPGVVVVER
                     DRALDQVREVVSSLGLEGPLDAFSRCSLCNEPLVEETPEGVSGLVPDHVLATRDRFAR
                     CPSCGRVYWKGTHLDRARGTMEAVFRDREP"
     gene            complement(468..845)
                     /locus_tag="PLT69_09670"
     CDS             complement(468..845)
                     /locus_tag="PLT69_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOJ14747.1"
                     /translation="MCPRSARDRGVEGERRAREYLVSRGYRILDENFRTRRGEIDIVA
                     SNGREVVFVEVKSAGSVSFGDPLCWVPRWKQERIALASAVYLSRKRMDDTPVRFDVIA
                     VGPSGAVRHVQDAFRPQSPQFRL"
     gene            complement(835..1425)
                     /locus_tag="PLT69_09675"
     CDS             complement(835..1425)
                     /locus_tag="PLT69_09675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ14748.1"
                     /translation="MSLEDELRAQGHWPLCGVDEAGRGPLAGPVVAAAVVLPPGSPLR
                     HVVMDSKSLSPSRRNAIHRLLVSSPEVRWSVSAVDPSTIDRVNILRATLTAMRQAVLN
                     LGVQPAFVLVDGNATPDLDCPCMAVVRGDASEPSIMAASIIAKVTRDGIMADMERLYP
                     GYGFARHKGYPTREHREALARLGACPIHRRSFRGVS"
     gene            complement(1427..1771)
                     /gene="rplS"
                     /locus_tag="PLT69_09680"
     CDS             complement(1427..1771)
                     /gene="rplS"
                     /locus_tag="PLT69_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOJ14749.1"
                     /translation="MDILDAFEKAQMKEGLPEIRIGDKVRVSTKIVEGSRSRSQVFEG
                     IVIARHRGNNRESITVRKQSYSVGVERVFPLHAPSVEKIEVVSHSKVRRAKLYYMRGR
                     KGKQARMKEIRS"
     gene            complement(1781..2302)
                     /locus_tag="PLT69_09685"
     CDS             complement(1781..2302)
                     /locus_tag="PLT69_09685"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ14750.1"
                     /translation="MLTVALIHWPCLDKNGREVATAITNLDIHDCARACLTYGVDTLY
                     IVHPNPGQRAFAERLMDHWLTGGGARYNAYRREALSIVRVVPNVEDIRSATKASLIGT
                     SAHRFPGSITWAEAKRRVSEGDVCMVFGTGWGMARRLVESLDAVVEPIHGKGAFNHLS
                     VRSAVAIALDRVT"
     gene            complement(2296..>2554)
                     /locus_tag="PLT69_09690"
     CDS             complement(2296..>2554)
                     /locus_tag="PLT69_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOJ14751.1"
                     /translation="TDSLATGDSHYAGLLEHPHYTRPRVFRGLSVPEVLVSGDHEAIR
                     RWRREKAVERTRKVRPDLLVCRPGDPGDQGHERDTGGNDRC"
BASE COUNT          428 a          876 c          782 g          468 t
ORIGIN      
        1 ccaaggagct tctcggtgag ttctctcttc gtcatggctc tctgtccctg aagacggcct
       61 ccattgtacc acgggcgcgg tcgagatgtg tacccttcca gtagactcgc ccgcaggagg
      121 gacacctggc gaaacgatcc ctggtggcaa ggacgtggtc ggggacgagc ccgctcacac
      181 cctctggggt ctcctccacg aggggttcgt tgcacaggga gcaccgggaa aaggcatcga
      241 ggggaccttc caggccgagc gaggagacga cctccctcac ctggtcgagg gccctgtctc
      301 gctctacgac cacgacccca ggtctgcccc tcagggccct cctcctcgtg agcactatcc
      361 tcccggacag ggcggcgcca aggagcctgt gcgggtcatc gtccctgagg tagagcgtat
      421 cgaagcccat gagtctcaac cacttcgcga gcctgccgag gttcctgtca caggcgaaac
      481 tggggggatt ggggcctgaa ggcatcctgc acatgcctca ccgcgccgga cggtccgact
      541 gcgatcacat cgaatcgcac cggcgtgtca tccatcctct tcctgctcag gtaaaccgcc
      601 gaagccaggg cgatgcgctc ctgcttccac ctgggcaccc agcagagcgg gtcgccgaag
      661 gagaccgacc cggcggactt gacctccacg aagacgacct cgcgcccgtt ggaggccacg
      721 atatcgatct cccccctgcg ggtcctgaag ttctcatcca gtatgcggta cccccgggag
      781 accaggtact ccctggcacg acgttcaccc tcgaccccac ggtccctggc cgacctagga
      841 cacacccctg aaggacctcc tgtgtatggg acaggcaccg aggcgtgcaa gggcctccct
      901 gtgttcccgg gtcgggtatc ccttgtgcct ggcgaagccg tagcccgggt agagccgttc
      961 catgtccgcc atgatcccgt ctctcgtgac cttggcgatg atgctcgccg ccatgatgga
     1021 gggctcgctc gcatcccccc tgacgaccgc catgcaggga cagtcgagat cgggtgtcgc
     1081 gttgccgtcc acgagcacga aggctggttg gacccccagg ttgagcacgg cctggcgcat
     1141 ggccgtcagc gtcgcccgga gaatgttgac gcgatcgatg gtggaggggt cgactgcgga
     1201 gaccgaccag cgaacctcgg gcgaggacac gagaagcctg tggatggcgt tgcgcctcga
     1261 ggggctcagg gacttcgaat ccatcaccac gtgcctgaga ggggaacccg gcggcaagac
     1321 gacggcagcg gccaccaccg gaccggcaag cggccccctg cctgcctcgt ccactccgca
     1381 gagcggccag tggccctgtg ccctgagttc gtcttcgaga gacatctcag gagcggatct
     1441 ccttcatgcg cgcctgcttt cccttgcggc cccgcatgta gtagagcttc gcgcgcctga
     1501 ccttggagtg gctgacgacc tctatcttct ccacggaagg cgcgtgcagg gggaagaccc
     1561 gctccacccc gaccgaatag gactgctttc ggaccgttat ggactcgcgg ttgttacccc
     1621 tgtgccgggc gatcacgatg ccttcgaaga cctgggaacg tgaacgcgat ccctccacga
     1681 tcttcgtcga gacgcggacc ttgtcgccga tccgtatctc cgggagccct tccttcatct
     1741 gcgccttttc gaatgcgtca aggatgtcca tcgttccctc ctatgttacc ctgtcaaggg
     1801 cgatcgccac ggcgctgcgc accgagaggt ggttgaaggc gcccttgccg tggatgggtt
     1861 ccaccacggc atcgagggac tccaccaggc gcctcgccat accccacccg gtgccgaaga
     1921 ccatgcacac gtccccttcc gaaacacggc gtttcgcctc cgcccaggtg atcgacccgg
     1981 gaaaacggtg cgcagaggtc cctatgagag acgccttcgt ggcactccgg atgtcctcga
     2041 cgttcggcac cacccgcacg atcgagagcg cctccctgcg gtacgcgttg tatcttgcgc
     2101 cgccgcccgt gagccagtgg tccatcagcc tctcggcaaa ggcccgctgg ccagggtttg
     2161 ggtgaacgat atagagtgta tcgactccgt atgtcaagca cgctcttgcg cagtcgtgga
     2221 tatcgaggtt cgtgatcgcg gtcgcaacct cgcggccgtt cttgtccagg cagggccagt
     2281 ggatgagggc gaccgtcagc accgatcgtt cccgcccgtg tctctctcgt gcccctgatc
     2341 cccggggtcg ccgggtcgac acacgaggag atccggcctg accttccgtg tcctttcgac
     2401 ggccttctcc cggcgccacc tccgtatggc ctcgtggtcc ccggagacga gcacctcggg
     2461 cacgctcaag cccctgaaca ccctgggcct cgtgtagtgg gggtgctcga gaagccctgc
     2521 atagtgagag tcccccgtgg cgaggctgtc cgtc
//
LOCUS       DAOSCR010000412         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26488_length_2552_cov_3.316780, whole genome shotgun sequence.
ACCESSION   DAOSCR010000412 DAOSCR010000000
VERSION     DAOSCR010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26488_length_2552_cov_3.316780"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLT69_09695"
     CDS             complement(<1..577)
                     /locus_tag="PLT69_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOJ14752.1"
                     /translation="MKREETESAFMGKHRSQANASFSRSAFVTAVTLAAGAVPALCLA
                     SEGPGHGLGILPALVLGVVEGITEYLPVSSTGHLYLASRLLGLGATPEGRAATDAYVI
                     AIQIGAILAVLWLYPGRVASMIRGCLGRDRQGLTLALNTLAALLPAAAIGLALEDLIK
                     ERLFATGPVTLAWLAGGVAILLVARKMNRDPA"
     gene            complement(574..1251)
                     /locus_tag="PLT69_09700"
     CDS             complement(574..1251)
                     /locus_tag="PLT69_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ14753.1"
                     /translation="METLQLMKKAPLFDGVGERILRMISQRCVVKSYDPGEAILNETE
                     PSSGLSVVLEGRVKLSKISQEGREQTLTVLAAGDPFGLCTAFASNDFPANVVALERST
                     ILTIPGSLLQETAREEPLLLLNVIRILSGRLRETMDLVENIALKDLPQRIASFLLHEA
                     AKKSCTQGCTIDIGMSHRELAKIVGATPEALSRALRRMSDEGVLKVEGRVIRLIDLEA
                     LKGLSGG"
     gene            complement(1440..2453)
                     /locus_tag="PLT69_09705"
     CDS             complement(1440..2453)
                     /locus_tag="PLT69_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ14754.1"
                     /translation="MRRTTRGRLLLAGLLALGLAVFVTAPMVGMKAVTVQDVLGNALD
                     VTAHKIFWEIRVPRVLVSFLAGAGLGLSGMAFQAMFRNVLATPYTLGVASGASFGAAL
                     CVRAGITLDLPLFSAVTLCAFAGAMLAVGVVYGISRSRAGMSPPTLLLAGVAVSFLFS
                     SLILFVQYTSGLTQSFRIVRWLMGSIDVLGYRPAVEIAPFVITGSLIVLALYRELDLM
                     LLGDELSMSRGLNVDRTRKLLVLAVSLMVGGIVAVCGPIGFVGMMAPHMVRLATGADH
                     KYLGPATFLFGGAFLTICDTMGRTLAAPAEIPVGVVTALLGGPFFLWLLLRRSGSGWW
                     GLG"
BASE COUNT          467 a          832 c          843 g          410 t
ORIGIN      
        1 tcgcaggatc gcggttcatc tttcgtgcca ccaggaggat ggcaacgccc cccgccagcc
       61 aagccagggt gaccgggcct gttgcgaaga ggcgttcctt gatgaggtct tcgagggcga
      121 ggccgatggc tgcggccggc aagagagccg ccagcgtgtt cagggccagt gtaagaccct
      181 gcctgtcccg gcccaggcag cccctgatca tggacgccac cctgcccggg tagagccaca
      241 ggaccgcgag gatggctcct atctggatgg cgatgacgta tgcgtcggtt gctgccctgc
      301 cctcaggcgt cgcgccgaga cccagcaggc ggctcgccag gtacaggtgc ccggttgagc
      361 tcacgggcag gtactcggtg atgccctcga ccacccccag gacaagggcg ggcaggatgc
      421 ccaggccgtg gcccgggcct tcggaggcga gacacagcgc gggtacggcc cctgccgcga
      481 gcgtcacggc cgtcacaaac gcggatcttg aaaagcttgc attcgcctgg cttcggtgtt
      541 tacccatgaa ggcgctctcg gtttcttccc tcttcatcct cccgagagcc ccttcagcgc
      601 ttccaggtcg atcaggcgga tcaccctccc ttccaccttc aggacccctt catcggacat
      661 ccgcctgagc gccctggaca gggcctccgg tgtcgctccc acgatcttgg cgagctccct
      721 gtgggacatg ccgatatcga tggtacaccc ttgcgtgcag gacttcttcg cggcctcgtg
      781 gaggaggaac gatgcgatgc gctgcggcag gtccttgagg gcgatgttct ccacgagatc
      841 catggtctcc ctgaggcggc cggacaggat cctgatgaca ttgaggagca ggaggggttc
      901 ctccctcgcc gtttcctgga ggagagagcc gggaatggtg aggatcgtcg aacgttcgag
      961 ggcgaccacg ttcgcgggga agtcgttcga ggcgaatgcc gtgcacagcc cgaagggatc
     1021 gcctgcggcc aggaccgtca aggtctgctc cctcccttcc tgggagatct tggagagctt
     1081 cacccggcct tcgagcacca cggagagacc gctcgagggc tcggtctcgt tgaggatcgc
     1141 ctcgcccgga tcataggact tgacgacgca gcgctgcgag atcatccgga ggatcctttc
     1201 ccccaccccg tcgaagaggg gcgccttctt catgagctgt agcgtttcca cttccccgcc
     1261 cgtcctctgc tctcggctgg tatccggcaa acccgtaccg ccgtcgtcga tggagtacca
     1321 tgattcccaa ggctcggtaa aggcgagatg acgagagatc atcaggggtc gtgcaaagcg
     1381 gcggaggctg tcgtcgatga tgcacgcccg ttccaagggg cccctttcgc cccggggcct
     1441 caacccaagc cccaccaccc tgatccggac ctccggagga ggagccagag gaagaagggc
     1501 ccgccgagga gggcagtgac tacgccgacg gggatctcgg ccggcgcggc gagcgtcctg
     1561 cccatggtgt cgcagatggt gaggaaggcg ccgccgaaga ggaaggtggc aggccccagg
     1621 tacttgtggt ccgcacctgt cgcgagccgc accatgtgcg gggccatcat gcccacgaac
     1681 cctatggggc cgcacacggc cacgataccc cctaccatga gggaaacggc gaggacgagg
     1741 agcttcctcg tcctgtccac gttgagcccc ctgctcatgg agagctcgtc gccaagcagc
     1801 atgaggtcga gctcacggta cagggcaagg acgatcaggg atcccgtgat cacgaaaggc
     1861 gcgatctcga cggccggccg gtacccgagg acatcgatgg aacccatgag ccacctcacg
     1921 atcctgaagg attgcgtgag cccgctcgtg tactgcacga acaggatgag gctcgagaag
     1981 aggaagctca ccgcaacgcc cgcgagcagg agcgtgggtg gggacatccc ggcgcgggac
     2041 ctcgagatcc cgtagacgac gccgacggcg agcatcgccc ctgcaaaggc gcagagagtg
     2101 accgccgaaa agagtgggag atcgagggtt atgcctgcgc ggacgcacag ggcggcgccg
     2161 aaggatgcgc cgctggccac ccccagggtg tacggcgtgg cgaggacgtt gcggaacatg
     2221 gcctggaagg ccatgccgct caggccgagg ccggctccgg ccaggaacga cacgagcacc
     2281 ctgggcaccc tgatctccca gaagatcttg tgcgccgtca cgtccaacgc gttcccgagg
     2341 acatcctgaa ccgtgaccgc cttcatgccg accatggggg cggtcacgaa aaccgcgagg
     2401 ccgagcgcca ggagccctgc gagcaggagc cttcccctcg tggttctcct catgcgtcct
     2461 ccgcgatcac gatcggtctc ccggtcgccg ggtgcctcag gacgaggaag gggatctcgt
     2521 acatcgacga gagggtcgcc tccacggtca tg
//
LOCUS       DAOSCR010000413         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_26582_length_2545_cov_3.212851, whole genome shotgun sequence.
ACCESSION   DAOSCR010000413 DAOSCR010000000
VERSION     DAOSCR010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26582_length_2545_cov_3.212851"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLT69_09710"
     CDS             complement(<1..471)
                     /locus_tag="PLT69_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14755.1"
                     /translation="MRVRCEKGALEKVSLRVFALAALCAACISPGARLHAAETREGGG
                     AFTLERFVEAVSAPLELLFRPTGPAARNVRKGPVPGRILFASNKDATQAVYLCEGGRV
                     TMICTNGADPSWSPDGTFFVCRPTASSVSWDLALVDTSGRLIRLLKPFGNGSATQ"
     gene            778..>2545
                     /locus_tag="PLT69_09715"
     CDS             778..>2545
                     /locus_tag="PLT69_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14756.1"
                     /translation="MKTRSLVPYLENPEAPFSLRIQTWGERVDIGSRPDLALVALEDR
                     SSGTRTLPGRVVSDAGGEVARVLLVMTADDHPLWAHETPGVSNPEVDEAFLEVYEWMR
                     DRGQLVPLASRQVGGTDRAAMGPLFVCTRTGRFASPLCPACGRALELVTDDRVLDEAR
                     LPSYSMSLERYLACRQCLGEGRGRFYAAHRRVDDPPCVSGVEDVVAGMAVLDAAGNPC
                     ASCAERSACHGGGEVTRRIVPVSFYPFRLFVLPDANIVAEDFLALASGAPVSQVCERL
                     AKRGQRIRKELVEKAVSFEGGLHLVEDGPGRFAETLLLKVSLMRDVAALALAPLEGKP
                     SSVYPPGLEQVWLHLPAGQPRLATLWHYRLASLGVHPRPTSSMLPASQVRDISLLGLL
                     WFDACISSKEVGRPALHDALVRMASDGRLAAENPLLDAGNVFWEKAGVEAFWMPLWER
                     ALKLGWSLLSAGPEFPRDAFVEECGLLVDDLRRSLVRGVAASLGDIPSPGRGRTVDHA
                     ADSEIAAILGEIRSAWAGGVGVRVRPGEESRPRPGARGRRLRVRMPISTPPSRPHPVE
                     RRWGGGQAPQAASCSVSPRRETT"
BASE COUNT          404 a          844 c          887 g          410 t
ORIGIN      
        1 ctgggtggcg ctcccgtttc cgaacggctt gaggagcctg atcagcctgc ccgaggtgtc
       61 caccaaggcc aggtcccacg atacgcttga agcggtgggc ctgcacacga agaacgtccc
      121 gtccggggac cacgaggggt cggcgccgtt cgtgcagatc atggtcaccc tgccgccctc
      181 gcagaggtac acggcctgtg tggcgtcctt gttcgaagcg aagaggatcc tcccgggcac
      241 gggccccttc cggacgttcc gggccgcggg gcctgtcggt cggaagagca gctcgagggg
      301 cgccgatacg gcctcgacga accgctcgag cgtgaacgcc ccgccgcctt cccgtgtctc
      361 tgcggcgtgg aggcgggcgc ccggagagat gcatgcggcg cacagcgccg cgagcgcaaa
      421 gacgcgcagt gaaaccttct cgagagcccc tttctcacat cgcaccctca tccatcactc
      481 cctccgagac tcattgcacc gcccgtgcgg cacggggtca agcagcgggg ggcatccggc
      541 acatactcta gcacagaccc atgaaacgca cacctgttgc ctgcgaggcg gcgccaaaga
      601 atcaggcgtc ggttacggaa agaaagggac agggcgcttg acaaccgagg tgcctggtta
      661 tagagtccag actgtaatgt attgaaatgc gtggatgata tacctttcct gcaccacgag
      721 cacggcgtgt tgcgcaacca cgagcacctt tcacccttct gagcggagct ttgccccatg
      781 aagacgcgtt cccttgtccc gtacctcgag aacccagagg cgccgttctc cctgaggatc
      841 cagacctggg gcgagagggt tgacatcggg tcacggcccg acctcgcact cgttgccctg
      901 gaggaccggt cgtcggggac ccgtacgctc ccgggacggg tcgtgagcga cgccggcggc
      961 gaggtggcga gggttctcct ggtgatgacg gccgacgacc accccttgtg ggcgcacgag
     1021 accccgggcg tatccaaccc ggaggtggac gaggccttcc tcgaggtcta cgaatggatg
     1081 agggacaggg gccagctcgt ccccctcgct tcacggcagg tcggcggcac ggaccgagcc
     1141 gccatggggc cgctgttcgt ctgcacccgc accgggcgct tcgcgagccc cctgtgccct
     1201 gcgtgcggga gggccctcga gctcgtaacg gacgacaggg tcctggacga ggcccggctc
     1261 ccgtcctact cgatgagcct cgagcggtac ctcgcctgca ggcaatgcct cggagaaggc
     1321 cgaggccgtt tctacgccgc ccacaggcgc gtggacgacc ctccctgcgt atccggtgtc
     1381 gaggatgtcg tggcgggcat ggccgttctc gacgccgcag ggaacccatg cgcctcgtgt
     1441 gccgagcgct ccgcctgcca cggcggcggg gaggtcaccc ggaggatcgt cccggtgtcg
     1501 ttctacccct tccgcctgtt cgtgcttccc gatgcgaata tcgtggcaga ggacttcctc
     1561 gcgctcgcaa gcggggcccc ggtctcacag gtctgcgaac gcctcgcgaa gagggggcag
     1621 aggatacgaa aggagctcgt cgagaaggcc gtgtccttcg aggggggcct gcacctggtc
     1681 gaagacgggc ccggcaggtt cgcagagacc ctcctcttga aggtctcgct catgagggac
     1741 gtggccgccc tggcgctggc acccctggaa gggaagccct catccgtcta cccgcccggg
     1801 ctcgagcagg tctggctgca ccttcccgcc gggcagccca ggctcgcaac cctctggcac
     1861 taccggctcg cctccctcgg ggtgcacccg agacccacat catcgatgct gcccgcatcg
     1921 caggtccggg acatcagcct cctgggcctg ctctggttcg acgcctgcat ctcgagcaag
     1981 gaggtcggaa ggcctgccct gcacgatgcc ttggtgcgca tggcctcgga tgggagattg
     2041 gccgccgaaa accccctgct ggacgcaggc aacgtcttct gggagaaggc cggcgtcgag
     2101 gcgttctgga tgccgttgtg ggaacgggcg ctgaagctcg gatggtctct cctctcggcc
     2161 gggccggagt tcccgcggga cgcgttcgtg gaggaatgcg gtctcctcgt cgacgatctc
     2221 cgccgctcgc tcgtgagggg ggtggccgcg agcctcgggg acatcccgtc tccaggaagg
     2281 gggcgaaccg tcgatcacgc cgccgattcg gagatagcgg ccattctcgg ggagatcagg
     2341 tctgcgtggg ccggaggcgt cggggtgcgg gtgcggcctg gggaggaatc gagaccgagg
     2401 ccgggggccc gagggcggcg gctgagggtg cgcatgccga tctcgacgcc accctcgcgg
     2461 ccgcatccgg tggagaggcg atggggaggg gggcaggcgc cgcaggcggc gtcctgctcg
     2521 gtgagccccc gaagggagac gactt
//
LOCUS       DAOSCR010000414         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27080_length_2511_cov_3.300896, whole genome shotgun sequence.
ACCESSION   DAOSCR010000414 DAOSCR010000000
VERSION     DAOSCR010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27080_length_2511_cov_3.300896"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="PLT69_09720"
     CDS             complement(<1..111)
                     /locus_tag="PLT69_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOJ14757.1"
                     /translation="MIRDLIVKNRSYRRFHEDQPVARSELLELVDLARLSA"
     gene            complement(101..844)
                     /locus_tag="PLT69_09725"
     CDS             complement(101..844)
                     /locus_tag="PLT69_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOJ14758.1"
                     /translation="MDMVPDPRRLIERLGIEWPVIGFYDAPTTDGFDPVVEPAPGRHT
                     CLFAFFKQWLQGKTLRLSQECYGCGGAGAALFSLQTRGREEFISFLADTEGLKCSHEV
                     MARWLDNRRTYKPTHGNILIGPLKDGRDEYLKTVTFFVNPDQLSALVIGAHYCHDLTG
                     QDPVLAPFGSGCMMLVTPFADLGEPQALIGATDIAMRPHIPEGLLAFTVTVPMYAMLC
                     GLDERSFLYKPFLKNLQKARRRREARDDT"
     gene            complement(914..1984)
                     /locus_tag="PLT69_09730"
     CDS             complement(914..1984)
                     /locus_tag="PLT69_09730"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ14759.1"
                     /translation="MKVLALNSSPRIKGQSKTEIMLSALVSGMEDAGAEVEVVHLRTK
                     TVKNCIGCYTCWTKTPGRCIHRDDMAEELLDKFISSDIAVFATPLYHFTMNATMKAFI
                     ERTLPVLEPFLEQKGDVTFHPLRSRHPAVVILSVAGFPEMSVFSQLSSHMRFMYRHGL
                     IGEIYRPAAEAMVLGPYTKQREDILAATKEAGTQLVRRMRVSEKTLERIGQPLGDKTV
                     MARAANIFWRTCMRERMTPEELRAKGVVPRPETIDDFVLIMKLAFRPENARGIDATIR
                     FDFTGQQEGSCSLTIHDGAVDGTTTPPDRADLAVKAPFELWMDVVTGRASGADLYLKG
                     AFKAEGDTNLLVRLGEIFGRNA"
BASE COUNT          403 a          818 c          811 g          479 t
ORIGIN      
        1 ggcggaaagc ctcgcgagat ccacgagctc aaggagctcg cttctcgcca cgggctggtc
       61 ctcgtggaat ctcctgtagg aacggttctt cacgatgagg tcacgtatca tctcttgcct
      121 cccggcgtct cctcgctttc tggaggttct tcaagaacgg cttgtagagg aagctcctct
      181 cgtcgaggcc gcagagcatg gcgtacatcg gcacggtgac cgtgaaggcg aggaggcctt
      241 cggggatgtg gggtctcatg gcgatgtcgg tcgcgcctat gagcgcctgg ggttccccga
      301 ggtcggcaaa gggtgtcacg agcatcatgc agcctgaccc gaagggtgcg agcacgggat
      361 cctggccggt gaggtcgtga cagtagtggg cgccgatcac gagggcgctc agctggtcgg
      421 ggttcacgaa gaacgtgacc gtcttgaggt actcatcccg cccgtctttc aaaggaccta
      481 tgaggatgtt cccgtgcgtg ggcttgtacg tcctgcggtt gtcgagccac cgggccatga
      541 cctcgtggct gcacttgagc ccctcggtgt cggccaggaa cgagatgaac tcctctctgc
      601 cgcgcgtctg caggctgaag agcgccgccc ccgcaccgcc gcacccatag cattcctggg
      661 aaagcctgag ggtcttccct tggagccatt gcttgaagaa cgcgaacagg caggtgtgcc
      721 tgccgggcgc cggctccacc accgggtcga acccgtcggt ggtcggcgcg tcgtagaacc
      781 cgatgaccgg ccactctatg ccgagcctct cgatgagcct cctgggatca ggtaccatgt
      841 ccatgcccct ttcactcctc gcttggctgc ggcccggccc cttcgtccag gccgcgcccc
      901 gggtgccgtg acgtcaggca ttcctgccga agatctcgcc caagcgcacc agcaggttcg
      961 tgtcgccctc ggccttgaag gcgcccttga ggtagaggtc ggcgccgctc gcccttcccg
     1021 tgaccacatc catccacaac tcgaaggggg ccttcacggc aaggtccgcc cgatcgggcg
     1081 gggtcgtggt cccgtcgacg gcgccgtcgt gtatggtcag gctgcaggaa ccctcctgtt
     1141 gcccggtgaa gtcgaacctg atcgtcgcgt cgatcccacg ggcgttctcc ggcctgaagg
     1201 cgagcttcat gatgagcacg aagtcatcga tggtctcggg cctcggcacg acccccttgg
     1261 ccctcagctc ctccggggtc atgcgctccc tcatgcacgt cctccagaag atgttcgccg
     1321 ccctggccat gacggtcttg tcccccaagg gctggcctat gcgttcgagg gtcttttccg
     1381 acaccctcat ccgcctgacg agttgtgtgc ccgcctcctt cgtggccgcg aggatgtcct
     1441 ccctctgttt cgtgtacggc cccagcacca tggcctcggc cgcgggcctg tagatctccc
     1501 ctatgagacc gtgccggtac atgaaccgca tgtggctcga gagctgggag aacaccgaca
     1561 tctccgggaa ccccgcaacg gacaggatca cgaccgccgg gtgcctcgaa cgcagggggt
     1621 ggaaggtcac gtcgcccttc tgctcgagga acggctccag cactggcagg gtgcgctcta
     1681 tgaaggcctt catggtcgcg ttcatggtga agtggtagag gggggtggcg aacacggcga
     1741 tgtccgacga gatgaacttg tcgaggagtt cctctgccat gtcgtcccgg tggatgcacc
     1801 tgccgggtgt cttggtccag caggtgtagc acccgatgca gttcttcacc gtcttcgtcc
     1861 tgaggtgaac cacctccacc tccgcgccgg catcttccat ccctgaaacg agggccgaga
     1921 gcatgatctc cgtcttgctc tgtcccttga tccgggggct tgagttcagt gccagtacct
     1981 tcatggggga tccctccgtt cagtgcatac tcggcccggg caaggcctgc gtcgccccgc
     2041 ccgtcagaca ggctaaccgt ttcacgaggc gttgacaaga ggcaagaaaa ccggcctcat
     2101 ggggggcctg cccaagggtg cgggtcaggc ggggaaacgt ggaagcaccg tcttttccgt
     2161 cctggacccg atatccgctg ccgcatgggg tgccctgcgg aaaagggtgc gcttcaccgg
     2221 tggtcgtcga gagggatggg tcgacgacac cgtcagcctc ccctggggcc tgcatgagaa
     2281 cggcgaggcc atgcttggcc cctgacaggc gagatcctcc tcgaggggaa aggggggccg
     2341 ctccgcctgc gcccgggatc gaggcatcgg ctgtggtgcg catggtcccg cacatgcgtc
     2401 caacgcgggc atgcccaccg tgcgcccacc cgggacaccc accccggtga tcgcgtagat
     2461 gcacgcgggg gctcgcaggt gcaaggcggc atggccgtct cacgggtacg a
//
LOCUS       DAOSCR010000415         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27237_length_2501_cov_2.705642, whole genome shotgun sequence.
ACCESSION   DAOSCR010000415 DAOSCR010000000
VERSION     DAOSCR010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27237_length_2501_cov_2.705642"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PLT69_09735"
     CDS             <1..1630
                     /locus_tag="PLT69_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HOJ14760.1"
                     /translation="IRASEFFIYTKKDRAALEACLALGYNFPRVTSWIRAHKEDLALV
                     KSMGIDETGILTSVSDYHIFLKLGTTRRKAMNDYLAIVSEALAMGITPRCHFEDVTRA
                     DIYGFCIPFARELMRLSEEAGMDVKIRLCDTLGFGVTFPGAALPRSVDKLVRAFIDDA
                     GVPPRLLEWHGHNDFHKVVVNATHAWLYGCRGVNAAIFGFGERTGNTPLEAMVFEYLS
                     LRGPDDSIDTRAITELAEYVRNEMGYHIPHNFPFVGKNFNVTRAGIHADGLVKNEEIY
                     NIFDTEKILGRPIEIAITDKSGKAGLVHWINSRFGLEGQRAIDKKHPGVTRMYRHIVK
                     EYEEGRNTSMSVEELEKLATRYVPHLFLSGFDLIKHEAQEYAAQLIERLLENEAMKSM
                     DPRKQEEVMAEFMKEFPFVQFAYVTDMRGVKITKNITQPEFRGQFLHKMRQDEDCSDR
                     DWFTKAVQTGDIYVSGVTTSRITGALIITVSSPIVGENEEMVGVLGLDIRFEDVIKAI
                     QSMYESWGIGMSPKDLQLYQRLLWEELHREHGTT"
     gene            1650..>2007
                     /locus_tag="PLT69_09740"
     CDS             1650..>2007
                     /locus_tag="PLT69_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ14761.1"
                     /translation="MEGCKITFDGSRLLVGDEPVIPFIEGDGVGPDIWAAARPVFDAA
                     VKKAYGGRRRVVWKEIYAGEKAMAMFGTALPDETIAALREYIVGIKGPLTTPVGGGIR
                     SLNVALRQMLDLFACIR"
     assembly_gap    2008..2017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2018..>2501
                     /locus_tag="PLT69_09745"
     CDS             <2018..>2501
                     /locus_tag="PLT69_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ14762.1"
                     /translation="RPVRYYAGTPAPVKRPDLVDITVFRENTEDLYAGIEWKEGTKEA
                     SRVIAFLKDEMGVSLREDSGIGIKPISKTGTRRLVRMAIQYALERGKKTVTLVHKGNI
                     MKFTEGAFRDWGYELAREEFGDVTVTEEELSTRYGGRLPDGKILVNDRIADSMFQQVL
                     L"
BASE COUNT          527 a          739 c          834 g          391 t
ORIGIN      
        1 catacgggcc agcgagttct tcatctacac gaagaaggac agggcggccc tcgaggcctg
       61 ccttgccctg gggtacaact tcccccgggt gacttcttgg atccgcgccc acaaggagga
      121 cctcgcgctg gtcaagtcaa tgggcatcga cgagacgggc atcctcacct ctgtctccga
      181 ttaccacata ttcctcaagc tcgggaccac caggcgcaag gcgatgaacg attacctggc
      241 catcgtgagc gaggctctcg ccatggggat cacgccgagg tgccacttcg aggacgtgac
      301 cagggcggac atctacgggt tctgcatccc gttcgcccga gagctcatga gactctccga
      361 agaggcgggg atggacgtga agattcggct ctgtgacacg ctggggttcg gtgtcacctt
      421 ccccggggcg gccctgccca ggtccgtcga caagctcgtg cgggccttca tagacgacgc
      481 aggggtgccc ccgcgcctcc tcgagtggca cggccacaac gacttccaca aggtcgtggt
      541 gaacgccacc cacgcctggc tgtacgggtg caggggggtg aacgcggcga tcttcgggtt
      601 cggggaaagg accgggaaca cccccctcga ggccatggtc ttcgagtacc tctcgctgcg
      661 gggcccggac gactcgatcg acaccagggc catcacggag cttgccgagt acgtcaggaa
      721 cgagatgggc taccacatcc cccacaactt cccgttcgtg gggaagaact tcaacgtcac
      781 cagggcgggc atccatgccg acgggctggt gaagaacgag gagatctaca acatcttcga
      841 caccgagaag atcctcggcc gtcccatcga gatcgctatc acggacaagt cgggcaaggc
      901 cggcctggtg cactggataa actcccggtt cgggctcgag ggacagcggg ccatagacaa
      961 gaagcacccc ggcgtcaccc ggatgtacag gcacatcgtg aaggaatacg aggagggcag
     1021 aaacacctcc atgtcggtcg aggagctcga gaagctcgcc acccggtacg tgccgcacct
     1081 cttcctgtca gggttcgacc tcatcaagca cgaggcgcag gagtacgccg cgcagctcat
     1141 cgagcggctc ctggagaacg aggccatgaa gtccatggac cccaggaagc aggaggaggt
     1201 gatggccgag ttcatgaagg agttcccgtt cgtccagttc gcatacgtca cggacatgag
     1261 gggcgtaaag atcaccaaga acatcaccca gccggagttc aggggccagt tcctccacaa
     1321 gatgaggcag gacgaggact gctcggaccg cgactggttc accaaggccg tccagacggg
     1381 cgacatctac gtgagcgggg tcaccacctc gcgcatcacc ggcgccctca tcatcaccgt
     1441 gagctcgccg atcgttgggg agaacgagga gatggtgggg gtgttggggc tcgacatccg
     1501 gttcgaggac gtgatcaagg cgatccagag catgtacgag tcctggggga tcggcatgag
     1561 cccgaaagac ctccagctct accagaggct cctctgggag gagctccaca gggaacacgg
     1621 caccacgtga gcgagggaag gatcaggaca tggaaggctg caagataacg tttgacggaa
     1681 gcaggctcct cgtcggggac gaaccggtga tccccttcat cgagggcgac ggcgtgggcc
     1741 cggacatctg ggcggcggca cggccggtgt tcgatgccgc cgtgaagaag gcgtacgggg
     1801 gcaggcgccg cgtcgtctgg aaagagatct acgcaggcga gaaggcgatg gcgatgttcg
     1861 gcacggccct gcccgacgag accatcgcgg ccctgaggga atacatcgtg ggcatcaagg
     1921 ggccgttgac cacgccggtg ggcggcggca tcaggagcct gaacgtggct ctcaggcaga
     1981 tgctcgacct gttcgcgtgc attcggcnnn nnnnnnncgg cccgtgagat actacgcggg
     2041 cacccccgcc cccgtgaaga ggccggacct cgtggacatc acggtgttca gggagaacac
     2101 cgaggacctc tacgccggga tcgagtggaa ggaggggacg aaagaggcct cgcgcgtgat
     2161 cgcctttttg aaggacgaga tgggtgtctc cctgagggag gattcgggca tcgggatcaa
     2221 gcccatttcc aagaccggga cgaggcgtct ggtacgcatg gcgatacagt acgccctcga
     2281 gaggggaaag aagaccgtga cgctcgtgca caagggcaac atcatgaagt tcacggaggg
     2341 ggcgttcagg gattgggggt acgagctcgc ccgcgaggag ttcggcgacg tgaccgtcac
     2401 cgaggaggag ttgtcgacac gctacggcgg ccgactgcca gacggcaaga tcctcgtgaa
     2461 cgaccgcata gcggactcca tgttccagca ggtgctcctg a
//
LOCUS       DAOSCR010000416         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27249_length_2500_cov_3.382822, whole genome shotgun sequence.
ACCESSION   DAOSCR010000416 DAOSCR010000000
VERSION     DAOSCR010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27249_length_2500_cov_3.382822"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLT69_09750"
     CDS             complement(<1..609)
                     /locus_tag="PLT69_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ14763.1"
                     /translation="MGKKRTERVPDASVVRALKNGDPHAMEELVRRYSNKVYNLAYHL
                     TRDANAAEEIMQDVFLTVISKVDTLANDAFFSTWLYRVTTNAAYGFLRKERKFGDQTA
                     IDEETEHEPSADYLHDWTKLPDSILLSEESRRILKESIDSLPEAMRTVVIMKDVEGLS
                     NEEMGEIFARWNKGMLESFLIEITAEIFPQVDDETGQPLVDLI"
     gene            complement(602..2098)
                     /gene="gcvPB"
                     /locus_tag="PLT69_09755"
     CDS             complement(602..2098)
                     /gene="gcvPB"
                     /locus_tag="PLT69_09755"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOJ14764.1"
                     /translation="MPDESPVTGFPVILEPLLAELSRPGSNPARTPRPFDAHLAEDLG
                     AYASPPRLPEVCEFETVRHFTRLSRLNYGIETGMYPLGSCTMKYNPKVSEVAAACVSD
                     IHPADAPAMERVLGWLADFREALLEITGMDDACLWPAAGAHGELAGMLVIGKALEARG
                     EKRRKVLVPDTAHGTNPASCTIAGFTTVNVPSGRSGCLEADQVARHLDADVAALMMTN
                     PNTLGVFERDIERIAGLLHDNGSYLYMDGANLNALLGVTRPGDMGVDCMHINLHKTFG
                     APHGGGGPGSGPVLVKADLSRFLPVPRLARDASGRARLMDDAPLSVGRIHGGLGNVAV
                     VLKAYAYVLSLGAQGLKDAAVRSCLNANYLRKRLEKTFGSVCPSPTLHEFVISDRAFR
                     DKGVSAMDIAKALMDKGFHPPTVYFPLNVQGALMIEPTESEPLRELERFALAMEEIAG
                     AAQVSPGDFGSHPMNTPVERVDEVWAARNLILTWHDMNPSSGGGYLNG"
     gene            complement(2077..>2500)
                     /locus_tag="PLT69_09760"
     CDS             complement(2077..>2500)
                     /locus_tag="PLT69_09760"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOJ14765.1"
                     /translation="DRLGRRAFCLTLATREQHIRREKATSNICTNQAHLALRAAVFLS
                     ALGPEGLRAVALQCASGARALRDALSSTPHRCVFSSPFFHEFAVRMDEATHARLMEAG
                     IVAGIPLGTDYPELEGCTLLCVTEMNTMEDIACLTSHL"
BASE COUNT          380 a          830 c          825 g          465 t
ORIGIN      
        1 gatcagatcg acaagcggct ggcccgtctc gtcgtccacc tgcgggaaga tctcagccgt
       61 gatctcgatc aggaacgact cgagcatccc cttgttccac cgagcgaaga tctcgcccat
      121 ctcctcgttc gacaggccct cgacgtcctt catgatcacg acggtcctca tggcctcggg
      181 cagggagtcg atggattcct tgaggatcct cctgctctct tccgacaaga ggatgctgtc
      241 cggcagcttc gtccagtcgt gcaggtagtc ggccgagggc tcgtgctcgg tctcctcgtc
      301 gatggcggtc tggtcgccga acttcctttc cttcctgagg aacccgtagg cggcgttcgt
      361 ggtgacccgg tagagccagg tcgagaagaa ggcgtcgttg gcgagcgtgt ctaccttgga
      421 gatcacggtc aggaagacgt cctgcatgat ctcctcggct gcgttcgcgt cccgcgtgag
      481 atggtaggcg aggttgtaga ccttgttcga gtaccgtctc accagctcct ccatggcatg
      541 tggatcgcca ttcttcaggg ccctcaccac ggacgcgtca ggcacgcgct ctgttcgttt
      601 cttacccatt tagatagccg cccccgctcg atggattcat gtcgtgccac gtcaggatga
      661 ggttcctggc cgcccacacc tcgtcgaccc tctccacggg cgtgttcatg gggtgcgaac
      721 cgaagtcgcc cggcgatacc tgggccgccc cggctatctc ctccatggcg agcgcgaacc
      781 tctcgagctc cctcaacggt tcgctctccg tcggttctat catgagcgcc ccctggacgt
      841 tgagcgggaa gtagaccgtg ggcgggtgga accccttgtc catgagcgcc ttggcgatat
      901 ccatggccga gacccccttg tccctgaatg cgcggtcgct gatcacgaac tcgtgcaggg
      961 tgggcgaggg gcagacgctc ccgaaggtct tctcgaggcg cttcctgagg tagttcgcgt
     1021 tgaggcacga acggacggcc gcatccttga gaccctgcgc gccgaggctc aacacgtacg
     1081 cgtacgcctt caggacgacg gcgacgttcc cgagcccgcc gtggatcctg ccgacgctca
     1141 ggggggcgtc gtccatgagc cttgccctgc ccgaggcgtc cctcgcgagg cgcggtacgg
     1201 ggagaaaccg cgagaggtcc gccttcacga gcaccgggcc gctgcccggc ccgcccccgc
     1261 cgtggggggc cccgaaggtc ttgtggaggt tgatgtgcat gcagtccacg cccatgtcgc
     1321 ccggccgtgt aacgcccagg agggcgttca ggttggcgcc gtccatgtag aggtacgacc
     1381 cgttgtcgtg gaggagcccg gctatcctct cgatgtcccg ctcgaagacg cccagcgtgt
     1441 tggggttggt catcatgagc gccgcaacgt cggcgtcgag gtgtctcgcg acctggtcgg
     1501 cctcgagaca gcccgaccgg cccgagggga cgttcaccgt cgtgaaaccc gctatggtgc
     1561 acgaagcggg gttcgtgccg tgggccgtgt ccgggaccag gaccttgcgc ctcttctcgc
     1621 ccctggcctc gagcgccttg ccgatgacca gcatgccggc gagctctccg tgggcgccgg
     1681 cggccggcca gagacaggca tcgtccatgc ccgtgatctc cagaagggcc tccctgaagt
     1741 ccgcgagcca gccgaggacc cgttccatgg caggggcgtc cgccgggtgg atgtcggaca
     1801 cgcaggcggc cgccacctct gagaccttgg ggttgtactt catggtgcac gaccccaggg
     1861 ggtacatgcc tgtctctatc ccgtaattga gcctcgagag cctcgtgaag tggcggaccg
     1921 tctcgaactc gcagacctcc ggcagccgcg gcggcgaggc gtaggcgccg aggtcttcgg
     1981 ccaggtgggc gtcgaagggc ctcggcgtgc gcgccgggtt cgacccgggc ctcgacagct
     2041 ccgcgagcag cggttccagg atcacgggaa agcccgtcac aggtgactcg tcaggcacgc
     2101 tatgtcctcc atcgtgttca tctcggtcac gcacagaagg gtgcatccct cgagttcggg
     2161 gtagtccgtc ccgaggggga tcccggccac gatccccgcc tccatgagcc tcgcatgcgt
     2221 ggcctcgtcc atcctgaccg cgaactcgtg gaagaagggc gaggagaaca cgcacctgtg
     2281 cggcgtggag gagagcgcgt ccctgagcgc cctcgctccg gatgcgcact ggagggcgac
     2341 ggccctgagg ccctcggggc cgagcgccga caggaagacg gctgccctga gcgccaggtg
     2401 ggcctggttc gtgcagatgt tcgaggtcgc cttctccctg cggatgtgct gttccctcgt
     2461 ggcgagcgtg aggcagaagg ccctcctgcc caagcggtcc
//
LOCUS       DAOSCR010000417         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27376_length_2492_cov_2.871974, whole genome shotgun sequence.
ACCESSION   DAOSCR010000417 DAOSCR010000000
VERSION     DAOSCR010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27376_length_2492_cov_2.871974"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLT69_09765"
     CDS             <1..364
                     /locus_tag="PLT69_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ14766.1"
                     /translation="NGRIEDLRLAAGFLKGNKVARGVRCLVLPATPEVYRQALKEGLL
                     DIFIEAGAIIGPPTCGPCLGGHMGILARGEKAIATTNRNFVGRMGDPSSEVYLSGPAV
                     AAASAVTGVITDPGEVKG"
     gene            361..855
                     /locus_tag="PLT69_09770"
     CDS             361..855
                     /locus_tag="PLT69_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOJ14767.1"
                     /translation="MKFKGRAFVFGDDVDTDAIIPARYLNTSDPRELAGHCMEDADPT
                     FARRVSPGDIIVAGKNFGCGSSREHAPIAIKASGVSCVIASSFARIFFRNSFNMGLPI
                     FECDEAVRGVKEGDILEVDADTGLITNLSAKKSFEARPIPAFMQELIADGGLMEHIRK
                     RGGI"
     gene            complement(883..1494)
                     /locus_tag="PLT69_09775"
     CDS             complement(883..1494)
                     /locus_tag="PLT69_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14768.1"
                     /translation="MRNPLHQGRMDGMCGIYAVMNGVSVLVQNRLSTSDLSDLFKELV
                     SALYRRNHSRRPRKSLPIEFIWEGTNVHEISLLLRTSKAFLALYDLELVWSRPLQGRS
                     RPSTVGQYWARLKKCFDDCEGRCVAILDYNFDGDEGEEGHWTCVEKMTERTMRLLDSS
                     LSIHSPRGVIRRSACTLGEKTPRRPYRLFPHNVYLVRLRQGDE"
BASE COUNT          446 a          757 c          874 g          415 t
ORIGIN      
        1 caacgggcgc atcgaggacc tgcgcctggc ggcaggcttc ctcaagggaa acaaggtggc
       61 ccggggggtg cgctgcctcg tcctgcccgc gacccccgag gtctaccgcc aggccctcaa
      121 ggaggggctt ctcgacatct tcatcgaggc cggggcgatc atagggccgc ccacgtgcgg
      181 accgtgcctc ggcggccaca tgggcatcct ggccagaggc gagaaggcga tcgccacgac
      241 gaaccggaac ttcgtgggca ggatgggaga cccatcgagc gaggtctacc tgtcggggcc
      301 ggccgtggcg gcagcctccg ccgtgaccgg cgtcatcacg gaccccgggg aggtgaaggg
      361 atgaagttca agggaagggc gttcgtattc ggcgacgacg tggacacgga tgccatcatc
      421 ccggcgcggt acctcaacac gtcggacccg cgggaactcg ccgggcactg catggaggat
      481 gccgacccga cgttcgcccg cagggtgagc ccgggagata tcatcgtggc gggcaagaat
      541 ttcggctgcg ggtcctcccg cgagcacgcg ccgatagcga tcaaggcctc cggggtgagc
      601 tgcgtgatcg cgtcgagctt cgcccggatc ttcttccgca acagcttcaa catgggcctg
      661 cccatattcg agtgcgacga ggcggtcagg ggcgtgaagg agggtgatat cctcgaggtg
      721 gacgcggaca ccggtctcat cacgaacctg agcgcgaaga agagcttcga ggcgaggccc
      781 atcccggctt tcatgcagga gctcatcgcc gacggcgggc tcatggagca catcaggaag
      841 cggggcggga tctgataccc cggggatccc cgcacccccc gctcactcgt caccctggcg
      901 caacctgacc aggtagacgt tgtgagggaa gaggcggtag ggtctccgcg gggtcttctc
      961 gcccagggtg caggcgcttc tcctgatgac gcccctcggg ctgtggatgc tcaaggagga
     1021 gtcgagcagc ctcatggtcc tctcggtcat cttttccacg caggtccagt ggccctcctc
     1081 gccctcatcg ccgtcgaagt tgtagtccag gatcgcgacg caccttccct cgcagtcatc
     1141 gaagcacttc ttgagcctcg cccagtactg tccgaccgtg gagggcctcg acctgccctg
     1201 gagcggccgg gaccagacga gctcgaggtc gtagagtgcg aggaaggcct tgctcgtacg
     1261 cagcaagagc gagatctcgt gcacgttcgt cccctcccag atgaactcga tgggcaggga
     1321 cttgcgcggt ctcctcgagt ggttgcggcg gtagagggcg gaaaccagct ccttgaagag
     1381 atccgacagg tccgaggtgg acaggcggtt ctgcacgagc accgacacgc cgttcatcac
     1441 ggcgtagatg ccgcacatgc cgtccattct gccctggtgg agggggtttc gcatgggaac
     1501 ctcaatcgcg gttttcagga ccgagttccc tacagactag tacagagcgt gacggaatgg
     1561 aagaggagag tgaagccccg cccgggtgcg gccggcaggc atgcaagggg gctgagcggc
     1621 atacggaaaa taaatggata tagcatcaaa aggtgggagg acagggaaga gccgttcggg
     1681 gggagagggg gcttggggcc gagggttttc ctcgtggggt caaaaggttg cgcgtgaacg
     1741 taagaggggg acagcgcatg ggcctgtttc gggaaggcct tcccctgggg agatatgcct
     1801 cacgcaaggg ggccgacaga aggccgcgcc tccccccttt cgcctgccat agggtatgcc
     1861 ccacgcaagg gggtaggctg cctcgaggcc gacctcttcg cccacaagag agggatatgc
     1921 cttacgcatg ggggtaggct gtttcttacc catggatatc ttccatgaac cgtcggatat
     1981 gcctcacgca tgggggccgg cccgaccttc gcaccctcgt tgagggaccg ggccgtggag
     2041 gtcctgggac agggggccac cgaccccagg gacgcctcac cggccgaggt cggggtgttt
     2101 ctcgagccct cggccggtct cgagcggcac tcaagaagca caggaggccc aaggtcgggg
     2161 tgcacgaggc ggggccgtcg gctgcggggg tcgaccggga gcgcgccctg agcgcgatgc
     2221 gaggggcggg cgggatgagc cttgcactcc ggggatgcgc ctcgtgaacc ctgccccaca
     2281 accccctgca tgcatcggcc attcagtttc gggtctttcc ggaggcatcg cctgtcgcgt
     2341 gccgggcggc atggccgcgc cgcatccttt cctgtggttg aaggtgacgt tttctgctac
     2401 atacgactca ccgacggagg tgatcgagac catgtcagac ggcttgaccg tggcggttgt
     2461 cgaaccggcg acgcccgtgg ggaaggagat cc
//
LOCUS       DAOSCR010000418         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27390_length_2491_cov_3.102217, whole genome shotgun sequence.
ACCESSION   DAOSCR010000418 DAOSCR010000000
VERSION     DAOSCR010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27390_length_2491_cov_3.102217"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="PLT69_09780"
     CDS             <1..1557
                     /locus_tag="PLT69_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOJ14769.1"
                     /translation="HQQVTDALAPAFGDVLVLSSIGDASTLIPMLASDSSSHEVAAFI
                     FNGLVKYDKDFNIVGDLARSWDIENEGKLLRFHLRNDVLWHDGEKFTAKDVMFTYRLI
                     VDPRTPTAYAEKYKLVRSARIVDEYTFEVEYDKPLAPALISWGSLQILPEHLLKGQDI
                     SKTPFARNPVGTGPFRFVSWQTGQKIVLKANETYFDGMPYLMGITYRVIPDQNTQFLE
                     LKAGAIDMMGLTPLQYLRQTSSPDFESKYNKYKYLSDGYTYLGFNLKRKPFDDVLVRR
                     AIAHAIDKREIIKGVLMGLGEVATGPYKPDTRWYNPSVKDFAYSPDSARALLMEAGYL
                     DRDGDGVVEKDGKPLSMTVLTNQGNPLREKTAQIIQQRLKAVGVDVRIRIIEWTVFLK
                     EYVDKGNFDAVILGWNILQDPDLFNVWHSSQAKKGGLNFVGYKDAEVDSLLEKGRQTF
                     DESQRKRCYDRIQEILADQQPYVFLYVPYSLPAVSSRIKGIVPAPAGITYNLEKWYVP
                     KHLQKYTILQ"
     gene            1569..2486
                     /locus_tag="PLT69_09785"
     CDS             1569..2486
                     /locus_tag="PLT69_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ14770.1"
                     /translation="MVRFLFRRLLVLVPTFIGITLVCFAVIHLAPGSPTDIQTQMNPD
                     ITPEAIEKLKAHYGLDKPLHVQYLVWMKNLCRLDFGPSMSSDARPVWDKIKERLPVTI
                     SINLASMLIILLAAIPLGVVSALTRASLLDRVITVVVFVGFSIPSFWLALVCMDLFGV
                     RLGWLPVSGIHSARYPDLSFAGMAADLARHLALPVLVSAFGSLAGMSRYMRSSMLEII
                     RQDYILTARAKGCSEFCLRYSHAGRNALLPVTTATGLRIPQLITGALRRAAPRWRIHD
                     PLAGLTPRSRALYFRASGRIGGRGGALGA"
BASE COUNT          478 a          870 c          731 g          412 t
ORIGIN      
        1 caccagcagg taacggatgc gcttgcgcca gcgttcggcg acgtcctcgt gctctcctcc
       61 atcggcgatg cgagcaccct gatccccatg ctcgcgagcg acagctcctc ccacgaggtg
      121 gcggccttca tcttcaacgg cctcgtcaag tacgacaagg acttcaacat cgtgggcgac
      181 ctcgcccgat cctgggacat cgagaacgag ggcaagctcc tgcggttcca cctgaggaac
      241 gacgtgctgt ggcacgacgg ggaaaagttc acggcgaaag acgtcatgtt cacctacagg
      301 ctcatcgtgg acccccgcac accgaccgcc tacgccgaga agtacaagct cgtcaggagc
      361 gcccgcatcg tcgacgagta caccttcgag gtggagtacg acaagcccct ggccccggcg
      421 ctcatcagct gggggtccct ccagatcctg cccgagcacc tgctcaaggg ccaggacatc
      481 tcgaagacgc cgttcgccag gaacccggtc gggacggggc ccttcaggtt cgtcagctgg
      541 cagaccgggc agaagatcgt gctcaaggcc aacgagacct acttcgacgg catgccctac
      601 ctcatgggca tcacgtaccg cgtcataccg gaccagaaca cccagttcct cgagctcaag
      661 gcgggcgcca tcgacatgat ggggctcacc ccgctccagt acctccgcca gacctcatcc
      721 ccggacttcg agtcgaagta caacaagtac aagtacctgt cggacggcta cacgtacctg
      781 gggttcaacc tgaagagaaa acccttcgac gacgtgctcg tccgcagggc catcgcgcac
      841 gccatagaca agcgcgagat catcaagggc gtgctcatgg gcctgggaga ggtggccacc
      901 ggcccctaca agcccgacac ccggtggtac aacccctcgg tgaaggactt cgcgtacagc
      961 ccggacagcg cccgcgccct cctgatggag gccggctacc tcgaccgtga cggcgacggc
     1021 gtcgtggaga aggacggcaa gcccctgagc atgaccgtgc tcaccaacca gggcaacccc
     1081 ctgagggaga agaccgccca gatcatccag caacggctca aggcggtggg cgtcgacgtg
     1141 aggatccgca tcatcgagtg gaccgtgttc ctcaaggaat acgtggacaa gggcaacttc
     1201 gacgcggtga tcctcggctg gaacatcctc caggaccccg acctgttcaa cgtctggcac
     1261 tcgagccagg cgaagaaggg cggcctgaac ttcgtggggt acaaggacgc ggaggtagac
     1321 tccctgctcg agaaggggag gcagaccttc gacgagtccc agaggaaacg ctgctacgac
     1381 cggatccagg agatactggc ggaccagcag ccctacgtgt tcctctacgt gccctacagc
     1441 ctgcctgccg tgagctcccg gataaagggc atcgtccccg cgcccgcggg gatcacctac
     1501 aacctggaga agtggtacgt gcccaagcac ctccagaaat acaccatcct gcagtaggga
     1561 aggcgccggt ggtccgtttc ctcttcaggc gcctgctcgt gctcgtcccc accttcatag
     1621 gcatcaccct ggtgtgcttc gcggtgatcc acctcgcacc cggatccccc acggacatcc
     1681 agacccagat gaaccccgac ataacgcccg aggccatcga gaagctcaag gcacactacg
     1741 ggctggacaa gccactccac gtccagtacc tcgtgtggat gaagaacctc tgccgcctcg
     1801 acttcgggcc gtccatgagc tcggacgcgc gccccgtctg ggacaagata aaagagcggc
     1861 tccccgtgac catctccatc aacctcgcct ccatgctcat catcctcctg gccgcgatcc
     1921 ccctcggggt cgtctcggcc ctcacgcgcg caagcctgct ggacagggtc atcacggtgg
     1981 tggtgttcgt ggggttttcc atcccgagct tctggctcgc gctcgtgtgc atggacctct
     2041 tcggcgtccg gctcgggtgg ctgcccgtct cgggcatcca ctccgcccgg taccccgacc
     2101 tgtctttcgc gggcatggcg gcggacctcg cccgacacct cgcgctgccg gtcctggtct
     2161 cggccttcgg gtcgctcgcg ggcatgagcc gctacatgcg ttccagcatg ctcgagatca
     2221 tacggcagga ctacatcctc accgcccggg ccaagggctg ctctgaattc tgcctgcgct
     2281 attcacatgc aggaagaaac gccctcttgc ccgtgaccac cgctaccggc ctgcgcattc
     2341 ctcagctgat cacaggcgcg ctgcgccgcg ccgcgccgcg gtggcggatc cacgatccgc
     2401 tggccggcct gacgccgcga tcgcgcgcgc tctacttccg cgcaagcgga cgcattggag
     2461 gacgcggtgg cgcattgggt gcgtgattac g
//
LOCUS       DAOSCR010000419         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27415_length_2489_cov_4.039441, whole genome shotgun sequence.
ACCESSION   DAOSCR010000419 DAOSCR010000000
VERSION     DAOSCR010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27415_length_2489_cov_4.039441"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLT69_09790"
     CDS             complement(<1..462)
                     /locus_tag="PLT69_09790"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOJ14771.1"
                     /translation="MRTIEDIRSAIDAIDDRILDLLVERSRLSVEMGKIKRSRNMLVF
                     DPSREEEILNRLTERCREPMTRFMVEGIFREIFSASRSLQRRQRVAYLGPEGSFSHQG
                     AFRVFSEDGDLVPFRDIESVVQAVASAQVDLGVVPVENSTEGMISQTLDVMA"
     gene            709..1899
                     /locus_tag="PLT69_09795"
     CDS             709..1899
                     /locus_tag="PLT69_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ14772.1"
                     /translation="MMDRIPIEDYAGPFKRRTRRFRLRYLLLPLVLLGVIFLFMPDGK
                     SGSEMIPPRGKPLGSRDLVHIDENVIRSHGLTIDPDLQRFVAETASKYKLHYGAMVVM
                     DAHSGAILAMYGKDVHGEDCRVCLKANHAASVFKIVTALAALENGFSPGSVFTYTGGA
                     HTLYKNQLVERRDRWTADITLADAFAKSNNVVFGKIGMHRLGESPILLAAMKLGFWKS
                     SLPEVDAEPSTLYLPEDPYALAELASGFNRKTKITPLHAAQMAAAAVNGGRLVTPRLV
                     RGSQPEAAQVMSPKTASMLADLMQETVRSGTLASEFRSARSDRVLKDITIGAKSGSID
                     GDDPPGRRNWFVGFALDEDTGNAISIGCLLVLNERFWIQADTFSRLVMHYYFSRSRVQ
                     QARS"
     gene            1959..2035
                     /locus_tag="PLT69_09800"
     tRNA            1959..2035
                     /locus_tag="PLT69_09800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1993..1995,aa:Pro,seq:ggg)
     gene            complement(2221..>2489)
                     /locus_tag="PLT69_09805"
     CDS             complement(2221..>2489)
                     /locus_tag="PLT69_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOJ14773.1"
                     /translation="ERTITGKDRTTYAYTIEVENRRGSAVHAVVMDQIPVSDDESVEV
                     RLTAATPKAEPDHNGFLRWDTTVEPGRRRTFSFEFSVTGARLTP"
BASE COUNT          449 a          809 c          786 g          445 t
ORIGIN      
        1 ggccatgacg tcgagggtct ggctgatcat gccctcggtg gagttctcga ccggcaccac
       61 cccgagatcg acctgggccg aggccacggc ctggaccacc gactcgatgt ccctgaaggg
      121 aacgagatcc ccgtcctcgg agaagaccct gaatgcgccc tggtgcgaga aactcccctc
      181 cgggcccagg taggccaccc tctgccgcct ctggagcgac ctggacgcgc tgaagatctc
      241 ccggaagatg ccctccacca tgaacctcgt catgggttcc ctgcatctct cggtgaggcg
      301 gttcaggatc tcctcctccc tcgagggatc gaagaccagc atgttgcgcg aacgcttgat
      361 cttccccatc tcgaccgaga gccgggagcg ctcgacgagg aggtcgagga tcctgtcgtc
      421 tatggcgtcg atggcggatc tgatgtcttc gatcgttctc attgtgaaag gccttgccgc
      481 cctcctcgat cacgtatgcc gggacacggc cagaaacagg gggcgcgtcc cctgggacct
      541 gccctggtgc atgccccgat gagagcgaaa cactaccaga acggtgggcc tttatcaagg
      601 ccgtgagcct tgccgatgga tctggatgtg agggatggag gcggagcgag gatgcgaggt
      661 tgacacgcac gatgggtata tgacataacc cgaggcaata acagtgaaat gatggatcga
      721 atccccatag aggattacgc agggcctttc aagcggcgca ccaggaggtt caggctccgc
      781 tacctgctgt tgccccttgt gctcctgggc gtcatcttcc tcttcatgcc ggacggcaag
      841 agcggctcgg agatgatccc ccccagggga aagcccctgg gcagtcgcga cctcgtccat
      901 atcgacgaga acgtgatccg cagccatggc ctcaccatcg atcccgacct ccagcgtttc
      961 gttgccgaga ccgcctcgaa gtacaagctg cactacggcg ccatggtggt catggacgcc
     1021 cactcgggcg cgatcctcgc catgtacggc aaggatgtcc acggtgagga ctgcagggtc
     1081 tgcctcaagg cgaaccacgc cgcgagcgtg ttcaagatcg tgacggcgct ggcggccctc
     1141 gagaacggct tttcgccagg gagcgtcttc acgtacacgg gcggggcgca caccctgtac
     1201 aagaaccagc tcgtcgagag gagggaccgc tggactgcgg acatcacctt ggccgacgcc
     1261 ttcgcgaagt ccaacaacgt ggttttcgga aagatcggca tgcaccgcct cggtgaatcc
     1321 ccgatcctgc ttgcggcgat gaagctgggg ttctggaagt cgtctctccc cgaggtggac
     1381 gccgaaccga gcacgctcta cctcccggag gacccctacg cccttgcaga actcgccagc
     1441 gggttcaacc ggaagacaaa gatcaccccc ctccacgccg cccagatggc cgcagccgcg
     1501 gtcaacgggg gacgcctggt gaccccgcgg ctcgtacggg gcagccagcc cgaggcggct
     1561 caggtcatga gcccgaagac ggcgagcatg ctcgccgacc tcatgcagga gaccgtaagg
     1621 agcggcacgc ttgcgagcga gttcaggtcc gcccggtccg accgggtcct caaagacatc
     1681 acgatcggcg caaagagcgg gagcatcgac ggcgacgatc cccccggcag gcgcaactgg
     1741 ttcgtgggtt tcgccctgga cgaggacacc gggaacgcca tctccatcgg atgcctgctc
     1801 gtgctcaacg agaggttctg gatccaggcc gacacgttct cccgtctcgt catgcactac
     1861 tacttctccc gctcgagggt gcagcaggcc aggtcgtgag gcgtcgaagc gcgaccttga
     1921 cacccgaaag actcgtgtgt tagcaagggt ggcggcttcg ggacgtggcg cagcctggta
     1981 gcgcacctga atggggttca gggggtcgga ggttcaaatc ctctcgtccc gaccaacact
     2041 tccaagaccc cgcctcacgg cttgctttat caaggggtgt ccagcccttg ctctctctcg
     2101 atcccgcagt gtccggtggg cttgcatcgc gcccctccat gagcgtggga gccgtcaacg
     2161 gggcggcccg ttcccgcggc cgagccgggg catggatgct tggggggctc gtgaagttcc
     2221 tcagggtgtc agccgagcgc ccgtgacgct gaactcgaag ctgaaggtgc gtctccggcc
     2281 cggctccacg gtcgtgtccc accgcaggaa gccgttgtgg tccggctctg ccttcggggt
     2341 ggcagccgtg agccgcacct cgactgactc gtcgtcagag acggggatct ggtccatgac
     2401 cacggcgtga acggccgagc ccctcctgtt ctccacctcg atggtgtatg cgtaggtcgt
     2461 gcggtccttc cccgtgatgg tcctctcgt
//
LOCUS       DAOSCR010000420         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27453_length_2487_cov_2.987253, whole genome shotgun sequence.
ACCESSION   DAOSCR010000420 DAOSCR010000000
VERSION     DAOSCR010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27453_length_2487_cov_2.987253"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..860
                     /locus_tag="PLT69_09810"
     CDS             30..860
                     /locus_tag="PLT69_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ14774.1"
                     /translation="MERGHMMDTVGQYLMENPEEALRLDVKTDPGAVRSQALLCGIGP
                     GARVLDGGCGSGKTTAVLCEMVQPGGSAVGVDFAEDRVRFAKKTYGDTPGIEFALMDL
                     RSPLNALGSFDFVWIRFVLEYYLEGAREMVENVSRVLKPGGRICLLDLDYNCLNHYPI
                     EPAMESVIHALVRRMMEKYNFDPYAGRKLYTHLYDLGFDDIEVHLVPHHLIYGELKES
                     DDFNWFKKLEMASSKARDVFDGYPGGYETFRKDFRQVFNHPRRFTYSPLIIVTGRKPS
                     "
     gene            1250..>2487
                     /locus_tag="PLT69_09815"
     CDS             1250..>2487
                     /locus_tag="PLT69_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14775.1"
                     /translation="MTDVHGKHVNDRRGRDNPERGYGAEAMSAVRSTRARMLTNPVRV
                     PLKRFFNLVNYHHFLDRTVFLLARDPRTKDEVLIPVHPGPCRGDLLSCTIEPGLREGA
                     WETVEPVSLVVDTGDILAVADVEAQDTAPGGITVRMVRDARLYTNRKTKRHACSNVYA
                     VIAFSGERVEGELREYHPGGLRVSLRGCSAETVERIRRERFVTLNLISAKEKVYAGTV
                     RCTRAEPEKGVVVFEPLNAPVRLCRERTHRNVRVNPDPAPRLAFVHPLTGRRVFFDIV
                     DLTTAGFSIVEKNDKALLMTGMTIRCAEIVFPGELALPCSFQVVYAKSLWGRKTRFGC
                     AITDMTPHTYTRLFNLVTRAIDPHSVVTSRVSLDSLWEFFFQSGFIYPEKYQCISGYK
                     EAFKATYEQLYHDCPDIFAS"
BASE COUNT          507 a          732 c          805 g          443 t
ORIGIN      
        1 acgcgtactt gggatcaggg gagagatcga tggaaagggg gcatatgatg gatacagtcg
       61 ggcaatacct catggaaaac cccgaagagg ccctgaggct cgacgtgaag accgaccccg
      121 gggccgtgag gagccaggcc ctcctgtgcg ggatcggtcc gggcgccagg gtcctcgacg
      181 gcgggtgcgg gtccggaaag accaccgccg ttttgtgcga gatggttcag ccgggcgggt
      241 ccgcggtggg ggtcgacttc gccgaggacc gcgtacgctt cgcgaagaaa acctacgggg
      301 acaccccggg gatcgagttc gccctcatgg acctgaggtc gcccctcaat gcgctcggct
      361 cgttcgactt cgtgtggata cgcttcgttc tcgagtacta cctggaaggc gcccgggaga
      421 tggtcgagaa cgtctcgcgg gttctcaagc cggggggcag gatctgcctc ctcgacctcg
      481 actacaactg cctgaaccat taccccatcg aacccgccat ggagtcggtc atccacgccc
      541 tggtgaggag gatgatggag aagtacaact tcgatccgta cgctggcagg aagctctaca
      601 cccacctgta cgacctcggg ttcgacgaca tcgaggtgca cctggtgccg caccacctga
      661 tttacggcga gctcaaggaa agcgacgact tcaactggtt caagaagctg gagatggcct
      721 cctcgaaggc cagggatgtc ttcgacgggt atcccggcgg atacgaaacc ttcaggaagg
      781 acttcaggca ggtcttcaac cacccgagaa ggttcacgta ctcgcctctc atcatcgtga
      841 cagggagaaa accatcataa gccggcatcg cgttcgtacg gtgtgcggaa atgtgataca
      901 atgcgccgag gcattccctc gacatgcccc tgcacctggg gggagcctgg acaccgggtg
      961 ccgattcccg gggtggaagg gaagcgaagg gtgaaggccc ctctcgaggc cgagccgtaa
     1021 gcggcacaga agatcgtgtg caggccggtc gcccggacac ctcttcaacg aacggcatcc
     1081 cagagaatga cgtgatctgg cacggatcgg gatatacccg gcaggaaggc cttcagccca
     1141 gggcagcatc ccttaaggcg gtgccgagat gcacgatggc ctggaagcgg gggcggcgtt
     1201 ggtgtacacg tacgggcatg gaatggggtt cactcaaggg ggctgtgaaa tgacagacgt
     1261 tcatggaaag cacgtcaatg accgtagggg gagggacaac ccggagcggg gatatggagc
     1321 agaggccatg tccgccgtgc gctccaccag ggcgcgcatg ctcaccaatc cggtgcgcgt
     1381 tccactgaaa cggttcttca atctcgtcaa ctatcaccat ttcctggaca ggacggtctt
     1441 cctgctcgcc cgggaccccc gcacgaagga cgaggtgctc atcccggttc accccggacc
     1501 gtgcagggga gacctcctct cgtgcacgat agagcccggc cttcgcgaag gggcgtggga
     1561 gacggtagaa cccgtctctt tggtcgtgga cactggcgac atcctggctg tcgccgatgt
     1621 cgaggcccag gacacggccc cgggcggcat cacggtcagg atggtccggg acgcgcggtt
     1681 gtacacaaac cgcaagacca agaggcatgc gtgctcgaac gtctatgccg tcatcgcctt
     1741 ttctggagag agggtggagg gtgaactccg cgaataccac cccggcggcc tgcgcgtgtc
     1801 tttgagggga tgctcggcgg agacggtcga gcgcataaga agagagcggt tcgtgaccct
     1861 gaacctcatc agcgctaagg agaaggtgta cgcgggtacg gtgaggtgca ccagggccga
     1921 acccgaaaag ggtgtggtgg tgttcgaacc gctcaatgca cccgtcaggc tgtgccggga
     1981 gaggacgcac cggaatgtcc gcgtgaaccc cgaccctgcc ccgcggctcg cgttcgtgca
     2041 tcctctcacg ggtaggaggg tgttcttcga catcgtggac ctgaccaccg ccgggttctc
     2101 gattgtcgag aaaaacgaca aggccctgct catgacgggc atgacgatcc ggtgcgcgga
     2161 gatcgtcttt cccggcgagc tcgcgctgcc gtgctctttc caggtcgtct acgccaagtc
     2221 tctctggggg aggaagacga ggttcggctg cgcgatcacc gacatgacgc ctcacacgta
     2281 cacgaggctg ttcaacctgg ttacccgtgc catcgacccc cactcggtcg tgacatcgag
     2341 ggtatccctc gattccctgt gggaattctt cttccagtcc gggttcatat accccgagaa
     2401 gtaccaatgt atcagcggtt acaaggaggc gttcaaggcc acctacgagc agctgtacca
     2461 cgactgtccg gatatcttcg cgagcat
//
LOCUS       DAOSCR010000421         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27593_length_2477_cov_4.110652, whole genome shotgun sequence.
ACCESSION   DAOSCR010000421 DAOSCR010000000
VERSION     DAOSCR010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27593_length_2477_cov_4.110652"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLT69_09820"
     CDS             <1..1239
                     /locus_tag="PLT69_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14776.1"
                     /translation="ERERERLQAQLAKADRLESLGRLAGGVAHDFNNMLGVIMGHAEL
                     ALEACGPHGALRPHLSEIRSASERSASIVRQLLAYARRQPRAPVLLDLNATVQSVLSM
                     IRTLIGENISLVWLPGKDLWSVEMDPAQVDQVVMNLCTNARDAISGQGTVVIETMNRS
                     FDAAYCTEHMECAPGDYVLLSVTDTGCGMDRDTSARIFDPFFSTKEPGSGTGLGLSTV
                     YGIARQNGGFINVYTEQGKGTTLKVYLPRSMKAAHDLAEPPGPASLEVQDRVHGLTVL
                     VVEDDRASLQTTSILLERMGHRVLGAQSPAEALVIASERGHAIDLILSDVIMPGMNGR
                     ELARRVEEMAPGIACLFMSGYTENIIVHRGVIEPGLHFIQKPFTRRELEVKMREALAD
                     SHGGAAPPRTGPVGREGRGP"
     gene            complement(1332..2225)
                     /locus_tag="PLT69_09825"
     CDS             complement(1332..2225)
                     /locus_tag="PLT69_09825"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ14777.1"
                     /translation="MERTVESLIARVHHLKTFSSVVMEIERLVSREDASLRDIAAVIE
                     KDPPTAAAVLRLANSSYYGLAQKVGSVTTALSVLGLHTVRGLVGMAAVVRAFAGATFT
                     LVKPELLWLHSLGCAASARVLAGEAGRGMQEEAFMAGLLHDIGLIVLLEAAEEDMVRV
                     KEALASGAFDSQAEAEREAIGFSHAEVGAALASRWHFPEPLVHAIRYHHSAQACEARA
                     RKNLPASVDPHRGMLGVFVFIADQLAKAMAMGDTVDPLVSPIPEGLWTLTGIPVEALG
                     RVMEEADELYEDLVDSLVFSA"
     gene            complement(2209..>2477)
                     /locus_tag="PLT69_09830"
     CDS             complement(2209..>2477)
                     /locus_tag="PLT69_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14778.1"
                     /translation="GVVLVAQGTVLSRALIEAIERKGIAQVFIEEEAACEWSAADLEA
                     MQASVEGEVAARFRERPSGALMEAIARAALSVEARKRLKGGANR"
BASE COUNT          435 a          856 c          809 g          377 t
ORIGIN      
        1 gaaagggaga gggaaagact ccaggcccag cttgccaagg ccgacaggct cgagtcgctg
       61 ggcaggctcg caggcggtgt cgcgcacgac ttcaacaaca tgctgggggt gatcatgggc
      121 cacgccgagc tcgccctgga ggcatgcggg ccgcacggcg ccctccggcc gcacctcagc
      181 gagatcaggt ccgcatccga gcggtccgca tccatcgtgc gccagctcct ggcctacgcg
      241 aggaggcagc caagggcgcc cgtgctcctc gacctcaacg ccacggtgca gtcggtcctg
      301 tccatgatca ggaccctgat aggcgagaac atctcgctcg tctggctgcc gggcaaagac
      361 ctgtggtcgg tggagatgga cccggcccag gttgaccagg tggtcatgaa cctctgcacc
      421 aacgcgcgcg acgcgatcag cggccagggc acggtcgtga tagagaccat gaaccggtcg
      481 ttcgacgcag cctattgcac ggagcacatg gaatgcgcgc cgggagacta cgtgctcctg
      541 tccgtcacgg acaccggctg cggcatggac agggacacct cggccaggat cttcgacccg
      601 ttcttctcca cgaaggagcc gggctccggg acaggactcg gcctctccac ggtctacggc
      661 atcgccaggc agaacggcgg gttcataaac gtgtacaccg agcagggcaa gggcacgacc
      721 ctcaaggtgt acctaccccg ctccatgaag gccgcccacg atctggccga gccccccggc
      781 cctgcatcgc tcgaggtgca ggacagggtc cacggtctca cggtcctcgt ggtagaggac
      841 gaccgggcga gcctccagac gaccagcatc ctcctcgaga ggatgggaca ccgggttctc
      901 ggggcccaga gcccggccga ggccctcgtg atagcctccg agcggggtca cgccatcgac
      961 ctgatcctga gcgacgtcat catgccgggc atgaacggca gggaactggc ccggcgcgtc
     1021 gaggagatgg cccccggcat cgcatgcctc ttcatgtcgg gctacacaga gaacatcatc
     1081 gtgcacaggg gggtgatcga gcctgggctg cacttcatcc agaagccgtt caccaggcgg
     1141 gagctcgagg tgaagatgcg cgaggccctg gccgactcgc atgggggagc cgctcccccc
     1201 cgcacgggcc cggtcggacg agaggggagg ggcccttgac ccggcagaag gacccgggac
     1261 gtacgggccg aacaaagatc gttcgagcag gtgggcggca ggcttccagc cgacctgagg
     1321 gcccctgacc ctcaggccga aaagaccagc gagtcgacga ggtcttcgta gagctcgtcc
     1381 gcctcttcca tgacgcggcc gagggcttcc acggggatgc cggtgagcgt ccacagcccc
     1441 tcggggatgg gagagaccag gggatccacg gtgtcgccca tggccatggc ctttgcgagc
     1501 tggtccgcaa tgaagacaaa caccccgagc atgcccctgt gcgggtcgac ggacgccggg
     1561 aggttcttcc tcgccctggc ctcgcatgcc tgtgcagaat ggtggtacct gatggcgtgg
     1621 acgaggggct ctgggaagtg ccacctggag gcgagcgccg ctcccacctc cgcgtggctg
     1681 aagcctatgg cctccctttc cgcttccgcc tgagagtcga acgcgccgga tgcgagcgct
     1741 tccttgacgc ggaccatgtc ttcctcggcc gcctccaaga gcacgatgag gccgatgtcg
     1801 tggagcaggc cggccatgaa cgcctcttcc tgcatacctc ggccggcctc gcccgcgagg
     1861 acccgggcgg aggccgcgca cccgaggctg tgaagccaca gcagctcggg cttgacgagc
     1921 gtgaaggtgg ccccggcgaa cgcccgcacg acagccgcca tgcccacgag ccccctgacc
     1981 gtgtgcaggc cgagcacgga gagcgcggtc gtgaccgacc ctaccttctg cgcgagcccg
     2041 tagtaactgg aattggccag cctcagcacg gccgccgccg tgggcgggtc cttctcgatg
     2101 accgccgcga tgtccctgag ggaggcgtcc tccctcgaga cgagcctctc tatttccatc
     2161 acgaccgagg agaacgtctt gaggtgatgg acccgcgcta tgagagattc aacggttcgc
     2221 tccacccttg agcctctttc tcgcctcgac ggacagtgca gccctcgcga tcgcctccat
     2281 gagggcgccc gagggccttt ccctgaacct cgcggcgacc tccccttcca cgctggcctg
     2341 catggcctcg aggtccgccg ccgaccattc gcaggccgct tcctcctcta tgaaaacctg
     2401 cgcgatcccc ttgcgctcga tggcctcgat caacgcccgg ctgagcacgg tcccctgcgc
     2461 gacgaggacc acccctg
//
LOCUS       DAOSCR010000422         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27606_length_2476_cov_4.171830, whole genome shotgun sequence.
ACCESSION   DAOSCR010000422 DAOSCR010000000
VERSION     DAOSCR010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27606_length_2476_cov_4.171830"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLT69_09835"
     CDS             complement(<1..332)
                     /locus_tag="PLT69_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ14779.1"
                     /translation="MLVFVVVGMPASGKNIAREYAAERGLPYFSTGDIVREEARRRGL
                     APDAGVMARLSDELRGPDGMGVTRLALEAALSKGAPAVFLEGMRSWPEIQLVASRARC
                     VVVAFVAP"
     gene            complement(1053..2030)
                     /locus_tag="PLT69_09840"
     CDS             complement(1053..2030)
                     /locus_tag="PLT69_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ14780.1"
                     /translation="MPIRPVMEVFRPIPVDVLLVGEPCVVDLFMKLDDTLVPFLSKGG
                     VLTGDHLRDLERLRVRRMFIKGSQAEAFEEYVQRNADKILTDPTVPSRVKAATFYISS
                     MGALKKAFVKPDAPSLNRIKKEIVPLLKGIMKNEIVLGDLLAITQHDFNTYTHSVNVG
                     IYATAIAVRFYRHDPSYGMNELERLSYGFFLHDIGKSRVPLSILRKRGPLSDEERSIM
                     KKHPEWGYAILMETGNLTDEAAYIAMQHHERPDGTGYPLGTKDIHPCARICALADIFD
                     ALTSKRPYKDPMSPFAALKLLKAETFTVFDHELLTTFIQILGPDKDVTH"
     gene            complement(2260..>2476)
                     /locus_tag="PLT69_09845"
     CDS             complement(2260..>2476)
                     /locus_tag="PLT69_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HOJ14781.1"
                     /translation="CPVARLERDDTVDPVQERIALFYERMSLLETGIQLFDSVYKTFI
                     GERLSKAWPETRRRIDAWLSSTGLAGR"
BASE COUNT          421 a          821 c          806 g          428 t
ORIGIN      
        1 ctcggcgcca cgaaggccac gaccacgcac cgcgcacgcg aggccaccag ctggatctcg
       61 ggccaggacc tcatgccctc gaggaacacg gccggggccc ccttcgagag ggccgcctcg
      121 agcgcgagcc tggtcacgcc catgccgtcc ggcccgcgaa gctcgtcaga caggcgcgcc
      181 atcacgcctg catcgggggc gaggccgcgt ctgcgggcct cctcccgcac gatgtcgccg
      241 gtggagaaat acggaagacc ccgctctgcg gcgtactccc tcgcgatgtt ctttcccgac
      301 gcgggcatac ccacgacaac gaataccagc atgatacctc ccctgggcag attcgttcac
      361 gggtggtcat catggaccac gcccgagccc tcgggcaagg ggaaatacac cgcccaaagc
      421 gcccgccgca cacaggcgga acttgcggca ttgctatctg gtgcggctgt ccggacaccg
      481 cgccgacctc acacaggctg agcatcatca tcggcattga gatcacggcc ctctgccacc
      541 tcgcaaaccg cacaaaggcg gggcgcctcc tggctcgcca gggacatttt ccgcgcaaaa
      601 cggctcacat gcggagccct gtccatgccc ccagggccgt gaccgatcat gcataaggca
      661 ccatgtcctg gggttatcgt gcaggcggcc gcctggtaca cccgcgcaga ccctctcctt
      721 ccttgcggtc acgacgacgt acagtcaccc tggggatcga tggacaaacc cgcccggggt
      781 gcgcggacgg tctgccgcgc aggcgtcctg ccggtggaaa gcgggcgggg acacgcctgt
      841 ccccggccga aggccgcgct gggtccgaag acccggcgaa ccgaaggcgg ccctgacacg
      901 gtcggtcgcc tcgcgcagga gcgcggggtg cgctgcactc atgtccctgt gcgtgcgaac
      961 ttcgaggcga ccggtggggc ctggacggcc ggcttcctgt aaccccgggc atgggggcaa
     1021 tgatgcgccg cctccggggg aagccggacg tctcaatgcg tgacgtcctt gtccggcccc
     1081 aggatctgga tgaaggtggt gaggagctcg tggtcgaaca cggtgaacgt ctccgccttg
     1141 aggagcttga gcgccgcgaa cggggacatg ggatccttgt agggcctctt cgaggtgagc
     1201 gcgtcgaaga tatcggccaa cgcgcagatg cgggcgcagg ggtggatgtc tttcgtgccc
     1261 agcgggtacc ccgtgccgtc cggccgctcg tggtgctgca tggcgatgta agcggcctcg
     1321 tccgtgaggt tcccggtctc catgaggatg gcgtagcccc actcggggtg tttcttcatg
     1381 atcgagcgct cctcgtcgga aaggggtccc ctcttcctga ggatggacaa ggggaccctg
     1441 gacttgccga tgtcgtggag aaagaacccg tagctcagcc gctcgagctc gttcatgccg
     1501 taggaggggt cgtgcctgta gaacctcacg gcgatggcgg ttgcgtagat acccacgttc
     1561 acgctgtggg tgtacgtgtt gaaatcgtgc tgggtgatgg ccaggaggtc tcccaggacg
     1621 atctcgttct tcatgatgcc cttgagaagg gggacgatct ccttcttgat ccggttcagg
     1681 gagggggcgt cgggtttcac gaaggccttc ttgagggcgc ccatcgacga gatgtagaac
     1741 gtggccgcct tcacgcgcga ggggaccgtg gggtcggtga ggatcttgtc cgcgttcctc
     1801 tgcacgtact cctcgaacgc ctcggcctgg gaacccttga tgaacatcct cctcacccgc
     1861 aagcgttcca ggtccctcag gtgatcgccc gtgagcaccc cacccttcga gaggaagggc
     1921 acgagcgtgt cgtcaagctt catgaagaga tccaccacgc agggctctcc cacgagcagg
     1981 acatccacgg gtatgggcct gaacacctcc atgacgggtc ttatcggcac gaccctttta
     2041 atatataaat attttaatat ccgccgccca ggccttgttc gaccgtctcc caggcgccgg
     2101 tacgcaccgc cccgggaggg cacggccttt gccgccttga cgcgctccgg cgccgggtca
     2161 cgggctcaac gaaggcggcg cccaggcgca tcggcgtgct tcgagacagg cgggcacccc
     2221 ccagcggccg tctcagggag agtgggcgcc gtcacggcct cacctccccg ccaggccggt
     2281 tgaggagagc caggcgtcga tccgcctcct cgtctcgggc cacgccttcg agagacgctc
     2341 cccgatgaag gtcttgtaca cgctgtcgaa gagctggatc cccgtttcca gcaggctcat
     2401 cctctcgtag aacagggcga tgcgttcctg cacggggtcc accgtgtcgt cgcgttcgag
     2461 acgtgcgaca gggcag
//
LOCUS       DAOSCR010000423         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27610_length_2476_cov_3.472532, whole genome shotgun sequence.
ACCESSION   DAOSCR010000423 DAOSCR010000000
VERSION     DAOSCR010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27610_length_2476_cov_3.472532"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1799)
                     /locus_tag="PLT69_09850"
     CDS             complement(303..1799)
                     /locus_tag="PLT69_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ14782.1"
                     /translation="MAYAHPDTSRSRDDLFDRIFHPQSVAVIGVSSRGGGFGSGILDS
                     LEAIGFEGRLYAVNARGGRYKGRKLYESVLEIPDEVDFAVIAVPAELVPEVLEECRVK
                     GVLASEILSSGFSETGTDEGKRLEERIAAISRTGIRVIGPNCFGVYCPKSGLTLLPGP
                     DLSRQSGEVAFLSQSGGMAIDLANIGTWLGLRFSKVVSYGNGVDLRETELLDYLGSDP
                     QTRVVGMYIEGVRDARAFLDVLTKVSAVKPVVVLKGGLSDAGARMVASHTGSLGGSRV
                     LWEAALRQAGAVVVDDIWDLAYTLLAFSMLPLRDYRGVCVAGGGGALGVSAGDAAERE
                     GLTLPAFDETVSDRILACLPRPGSSARNPVDAANPFVGPDAFDHVFRIAATDDRIDMH
                     LLIQLLHHYRPLARSMGVSLLEMVPYRELARVLAAVRDDTGKPVVLVMPDYRQGTDSI
                     DIGELIRHARQAFVEHGLPVFPDLSNALRATSRVSGYVRMLRARTSRP"
     gene            2108..>2476
                     /locus_tag="PLT69_09855"
     CDS             2108..>2476
                     /locus_tag="PLT69_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOJ14783.1"
                     /translation="MPKENTHLWFAYSMLGRSIGADMLRTVSSHLSSYLLGSVMPDTF
                     YYSPRRELVSISETFHGRDGNPTNTLVFKVLDAGPDQADLAFVLGFLTHCALDIAFHP
                     VVYFLTGNYYDPDPARKAQAV"
BASE COUNT          418 a          857 c          783 g          418 t
ORIGIN      
        1 cacttttcac gtgccaaagt tgtcatgaac cttccccaaa cgtgtcgata cgtttcttga
       61 caacgaagac tccgcatctg tgagacttct ccataggggg gccgggcggc tgggtccgcc
      121 cggcccgttg ttgtgggacc gggaggcgtc gacctggcac gtccgaggcc ggacccttga
      181 acgaccgccg agagctgttt cctcgtcatg cgccccagta tgtgtgcaca gggcggtgtt
      241 gcaggtggtg ctgtacgacc tgcaagagcg gcttccccct catgcgcccc aggccgggcg
      301 tatcacgggc gtgacgtgcg ggcccgcagc atgcgcacgt accccgacac gcggctcgtc
      361 gccctgagcg cattcgagag gtcggggaag acaggcagcc cgtgctctac gaacgcctgt
      421 ctcgcgtgcc gtatgagctc gccgatgtcg atgctgtccg tcccctgcct gtagtcgggc
      481 atcacgagga ccaccggctt gcccgtgtcg tccctcacgg cggcaaggac acgggcgagc
      541 tcccggtagg gtaccatctc gagcagggac acgcccatgg accttgccag cggcctgtag
      601 tggtggagca gctggatgag gagatgcatg tctatcctgt cgtcggtcgc ggctatcctg
      661 aacacgtggt cgaaggcatc ggggccgacg aacgggttcg cggcgtcgac ggggttgcgc
      721 gcgctggaac cgggtctcgg caggcaggcg agtatccggt cgctcacggt ctcgtcgaaa
      781 gcgggcagcg tcaggccctc gcgctcggcc gcatccccgg cgctcacgcc gagcgcgccg
      841 ccgccgcctg ccacacagac accccggtag tcccgaaggg gcagcatcga gaaggccagc
      901 agggtgtacg ccaggtccca gatgtcgtcg acgaccacgg cgcccgcctg cctgagggcg
      961 gcttcccaca ggacacggct gcccccgagg gaccccgtgt ggctggccac catcctggca
     1021 ccggcgtccg agagcccccc cttgagcacg accaccggtt tgacggccga cactttcgtg
     1081 aggacatcga gaaaggccct ggcgtctctc acaccctcga tatacatccc cacgaccctg
     1141 gtctgggggt ctgagccgag gtagtccagg agctcggtct ccctcaggtc caccccgttg
     1201 ccgtagctca ccaccttcga gaaccgcagg ccgagccacg tgcctatgtt ggcgaggtcg
     1261 atggccatgc cgccgctctg cgaaaggaac gccacctcac cgctctgcct cgacaggtcc
     1321 gggccgggca acagggtgag cccgctcttc gggcagtaca cgccgaagca gttggggccg
     1381 atgaccctga tgccggtcct cgatatcgcg gctatgcgtt cctcgagcct tttcccctca
     1441 tcggtcccgg tctccgagaa accggaagac aggatctccg aggcaagcac gcccttgacc
     1501 ctgcactcct cgagcacctc cgggacgagc tcggccggga cggctatgac ggcgaagtcc
     1561 acctcgtcgg ggatctccag cacgctctcg tagagcttgc ggcccttgta ccgacctccc
     1621 cgtgcgttga ccgcgtagag cctgccctcg aaccctatcg cctcgaggga gtcgaggatg
     1681 ccgctcccga agcctccccc tcggctggac acgcctatca ccgcgaccga ttgggggtgg
     1741 aagatgcgat cgaagaggtc atccctgctg cgcgaggtat cagggtgtgc gtatgccatt
     1801 cccggtgctc cctcgtcgtg ttgtatggca tacatagtga cagctgtgcg ccgcgatgtc
     1861 aacggtcacc gctgccggga cgcctcgggc acacaagggc gcgcctcact gggggtcggg
     1921 atgccggaac atgtggaggg agacaccccc tggaccgccc tgagacggga tcctgggggc
     1981 aaacggacaa ggccagcaga cgggcatcgg tcccgaggcg ccttgcagcc gcccccttcg
     2041 ccatcaggcc gctttacagc acgggcctga ccggatatag ttcggccagc acagggaagg
     2101 atcccgcatg ccgaaggaga acacgcacct ctggttcgca tacagcatgc tcggccggtc
     2161 cataggggcc gacatgctgc ggacggtctc ttcgcacctc tcgagctacc tcctcgggtc
     2221 agtcatgccg gacacgttct actacagccc ccgcagggag ctggtgagca tctcggagac
     2281 gttccacggc agggacggca accccaccaa cacgctcgtg ttcaaggtcc tcgatgccgg
     2341 gcccgatcag gccgacctcg cgttcgtgct cgggttcctc acccactgcg ccctggacat
     2401 cgcgttccac ccggtggtct acttcctcac cggcaactac tacgacccgg acccggcgag
     2461 aaaggcccag gcggtc
//
LOCUS       DAOSCR010000424         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27618_length_2476_cov_2.598926, whole genome shotgun sequence.
ACCESSION   DAOSCR010000424 DAOSCR010000000
VERSION     DAOSCR010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27618_length_2476_cov_2.598926"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="PLT69_09860"
     CDS             complement(<1..1602)
                     /locus_tag="PLT69_09860"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOJ14784.1"
                     /translation="MNSTCLRSWSVPRSAPRFSCFLVISAELVLLGLLVVCASGGIGG
                     ACEEQVSPEVVLGKNRPEGAACVVDWAMFTEIQEEPGCLEWYGPCEWTQDDMGEALFP
                     EGVTMLTLFTCDSGRRSDAHTARVVVEPCFDILAVPMKNKAVISWRKVTGASRYEVYR
                     AHESSPWDFVKAGEAPASAVSFTDTNLGDASYLYTVGAVVSGRYRFSRTRCVHPFASV
                     PRPNYPPVIHTKPVTCAVAGLPYTYEVGAEDPYREKVEYSLVAKPVGMTIDRTTGLIA
                     WKPTQVGDYEVTVKAKDCSGLSDTQTFIIEVDALPALNHNPVAEASGPYRGIPGGPMM
                     FDGSASYDPDGDALAYWWDFGDGSTGTGVKPVHSYSRPGTYTVTLTVSDQKGGVGSDT
                     ASAFVENCEAPKVDIEASPAAVRPGDPCTLVWSSEHASSVWIDNGIGGVAASGTLTVR
                     PLASTTYTIQAQGPCGSASDTVTVYVHQAPSVSIAADPASIARGQSATLSWTSSNALD
                     LSIDHGIGQVEPHGSTTVAPGETTTY"
     gene            complement(2068..>2476)
                     /locus_tag="PLT69_09865"
     CDS             complement(2068..>2476)
                     /locus_tag="PLT69_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ14785.1"
                     /translation="VDIEEKPKHPRSNCAVTGLYFYDNRVVDIAKNLKPSLRGELEIT
                     DVNREYLRMGELRVERLGRGFAWLDTGTHESLLEAGMFIETIEKRQGLKIACVEEIAY
                     RMGYIDAARLEAIAESMKNSSYGAYLLRQLHTP"
BASE COUNT          446 a          798 c          771 g          461 t
ORIGIN      
        1 gtaggtggtg gtctccccgg gcgcgaccgt ggtgctgccg tggggctcca cctgcccgat
       61 cccgtggtcg atggagagat ccagggcgtt cgacgaggtc caggaaaggg tcgcgctctg
      121 gccgcgggcg atagacgccg ggtcggccgc tatgctgacc gagggcgcct ggtggacgta
      181 caccgtgacg gtatcagatg ccgagccgca ggggccttgc gcctgtatcg tgtaggtcgt
      241 gctcgcgagg ggacgcacgg tgagagtccc ggacgcggcc acgccgccta taccgttgtc
      301 gatccacacg gatgaggcgt gttccgacga ccacacgagg gtgcacgggt caccgggacg
      361 gaccgctgcc gggctcgcct cgatgtcgac cttcggggcc tcgcagttct cgacaaaggc
      421 gcttgccgtg tcagagccta caccgccctt ctggtctgac accgtgaggg tcacggtata
      481 cgtgccgggc ctgctgtagc tgtgaacggg tttgacgccc gtccccgtcg acccgtcgcc
      541 gaaatcccac cagtaggcaa gcgcgtctcc gtccgggtcg tacgaggcgg agccgtcgaa
      601 catcatcggc cccccgggta tgcccctgta cgggcccgag gcctctgcca ctgggttatg
      661 gttcaacgcg ggcagtgcat cgacctcgat gatgaaggtc tgggtgtcgg aaagcccgct
      721 gcaatccttc gccttgaccg tgacctcgta atcccccacc tgggtgggtt tccacgcgat
      781 gaggccggtg gtcctgtcga tggtcatccc cacgggcttg gccacgagcg agtactcgac
      841 cttttcccgg tacgggtcct cggcaccgac ctcgtaggtg tacggaaggc ccgccaccgc
      901 gcaggtgacc ggttttgtgt ggatgacggg cgggtagttc ggcctcggca cgcttgcaaa
      961 cgggtgcacg cacctcgttc gggaaaaacg atacctgcct gagactacgg cgcccaccgt
     1021 gtagaggtac gaggcgtcac cgaggttcgt gtccgtaaag gagacggctg aagcaggcgc
     1081 ttcgcctgcc ttgacaaagt cccacgggct cgattcatgg gcacggtaga cctcgtaacg
     1141 cgaggccccc gtgaccttgc gccacgagat gaccgccttg ttcttcatgg gcacggcgag
     1201 gatgtcgaaa caaggctcaa cgaccaccct cgccgtgtgg gcatcggacc gcctcccgct
     1261 gtcgcaggta aacagggtga gcatggtcac gccctcggga aacagggcct cacccatgtc
     1321 atcctgggtc cactcgcagg ggccgtacca ctcgaggcac cctggttcct cctggatctc
     1381 ggtgaacatg gcccagtcca caacgcaggc agcgccctca ggcctgttct tgccgaggac
     1441 gacctcgggc gacacctgct cctcgcacgc cccgccgata ccgcccgagg cgcacaccac
     1501 gagcagcccg agcaggacga gttccgcaga gatcaccaag aaacacgaga acctgggggc
     1561 agacctgggg acactccaag accgcaaaca agtgctgttc atgaccgaca acctcctcaa
     1621 tgcaacgaaa cgtttccgag gaaggcgcaa tgaaaattac atgacccaac gaatacacta
     1681 tcatatcaac ccgtgtcaat gtctggtttg ccatgcccga aaggcccgta cccctcgtga
     1741 aaggacaaaa cggctcaggg cttgttgtat attcaggctg cgaggaggcc gggtacgcat
     1801 tcatcaaggc gcacgagggt gcaggagctt cagggctcag gacgtcttcc aggtgacatg
     1861 gctttcccgc ggccggtgtc gtcctcgggc tgaccagaac gcccaggctc tccaggtatc
     1921 agggcgatca tcacgagtga tggctgcacc cagcctgtcc cgcggacaag gcctcggcag
     1981 ggcggcacct acgggtgcat ctcatgcatg ggcgttgttc tgaggccggc ctcgtatcca
     2041 gcgaggcgca ggcctgagca ggcgctttca gggggtatgg agctgcctga gcaggtaggc
     2101 gccgtagctg ctgttcttca tcgactccgc tatggcctcg agccttgcag cgtcgatgta
     2161 gcccatgcgg taggcgatct cttcgacgca ggcgatcttg agcccctgcc tcttctcgat
     2221 ggtctctatg aacatgcctg cctccagaag cgactcgtgg gtgccggtgt ccagccacgc
     2281 gaatcccctt ccgagccgct ccaccctcag ctcgcccatc cggaggtact ccctgttcac
     2341 gtccgtgatc tcgagctcgc cccgcaagga cggcttcagg ttcttcgcga tgtccacgac
     2401 cctgttgtcg tagaaataca acccggtgac agcacagttc gaccttgggt gtttcggttt
     2461 ttcctcaatg tccacg
//
LOCUS       DAOSCR010000425         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27750_length_2466_cov_2.980091, whole genome shotgun sequence.
ACCESSION   DAOSCR010000425 DAOSCR010000000
VERSION     DAOSCR010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27750_length_2466_cov_2.980091"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1324)
                     /locus_tag="PLT69_09870"
     CDS             complement(344..1324)
                     /locus_tag="PLT69_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOJ14786.1"
                     /translation="MTAFIVFLIALALFFDFLNGFHDAANSIATVVSTRVLSPQYAVV
                     WAAFFNFIAFVFMGTHVAKTIGKGIVDVGIIDPTVIFATLVGACAWDILTWYFGLPTS
                     SSHALIGGLVGAALVKAGPGSLVAKGIAMTSAFIFISPAIGLILGLTLGVAVYWWFRK
                     ATPLHVDHIFRRGQLFSAALYSLGHGGNDAQKTMGIIAGLLFSAGLTRGTFHIPLWVV
                     LSCQAAIALGTMFGGWRIVKTMGQRITKLRPVDGFCAETGAAISLYLATYLGIPVSTT
                     HTITGSIMGVGSLRRLSAVRWGVAGRIVWAWILTIPGAALISAAAYLAAR"
     gene            complement(1329..1955)
                     /locus_tag="PLT69_09875"
     CDS             complement(1329..1955)
                     /locus_tag="PLT69_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOJ14787.1"
                     /translation="MGFSLLPKTTDFFVLFNKQAELAVRAATLFARQTRDGRFDAETV
                     NQMRDIEHQADEVAHDLMDRLNKTFITPFDREDIHNLACALDDVIDMIYTISNRMRVY
                     RIDTPDDTLVEFATIIEKSVKTLVTAVEGLKDPKMLQATKDACIEVNRLENVGDTMRD
                     ASLARLFENATDPIHIVKWKEIYAFAEEVLDLCEDVVHIVESILVKQA"
     gene            complement(2113..>2466)
                     /locus_tag="PLT69_09880"
     CDS             complement(2113..>2466)
                     /locus_tag="PLT69_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14788.1"
                     /translation="RTDPFLVDQILINLVDNAVKYTERGSITVRASKQGRTTTIEVTD
                     TGIGIPREHQRRIFERFYVVDKSRSRSTGGTGLGLSIARNAATILGGAIEVESMPGSG
                     TTFRVTLPDLEPRDA"
BASE COUNT          399 a          774 c          824 g          469 t
ORIGIN      
        1 ctcctgcccc cctccggaca gggacggtcg atgattgtat gccgtggctt agggcatccc
       61 ctcggcgggg agctttctga aggcggatgc tggatcagca gggctcgact cggggcgctg
      121 ggacgcttgg gtcccgtcgc cccacgcatg ccggcctcgt ggcggggacg ggggtcacac
      181 gccgtttccc ccggctccgg ctgcgaggcg cctcaggcgg ggcccacgag tcccgagatc
      241 aggctccttt cttctctcgg cccggcgccg ttcaggtccg ggatccggcg cgcagggcga
      301 ggtgggtcgc cttggttttc ggatggaggc ggtcatgccc gtttcatctg gccgccagat
      361 aggctgcggc agagatcagg gctgcgcctg ggatggtgag gatccacgcc catacgatgc
      421 ggcccgccac cccccacctg acggcgctca gtcgtcgcaa cgaccccacg cccatgatgg
      481 agcccgttat ggtgtgggtc gtgctcacgg gtatcccgag gtacgtcgcc aggtagaggc
      541 tgatcgccgc ccccgtctcc gcgcagaaac cgtcgactgg cctgagcttg gttatccgct
      601 ggcccatggt cttgacgatg cgccagccgc cgaacatggt gccgagggcg atcgccgcct
      661 ggcacgacag gacgacccag agggggatgt ggaaggtgcc gcgcgtgagc cccgcggaga
      721 acaggagacc ggcgatgatg cccattgtct tctgcgcgtc gttcccgccg tggccgagac
      781 tgtagagggc cgcggagaag agctggcccc tgcggaagat gtggtccacg tgcagagggg
      841 tcgccttcct gaaccaccag tagaccgcca ccccgagggt cagcccgagg atcaggccta
      901 tggcggggga gatgaagatg aaggcggagg tcatcgctat cccctttgcc acgagggacc
      961 ccggcccggc cttgacgagc gccgcgccca cgaggccgcc tatgagcgcg tgggaggagc
     1021 tcgtgggcag gccgaagtac cacgtgagga tatcccacgc gcaagcgccc acgagggttg
     1081 cgaagatgac cgtggggtct atgatcccca cgtccacgat ccccttcccg atggtcttgg
     1141 ccacgtgtgt ccccatgaag acgaaggcga tgaagttgaa gaaggcggcc cagaccaccg
     1201 cgtactgggg cgagaggacc cgggtcgaga ccacggtcgc tatcgagttc gccgcgtcgt
     1261 ggaacccgtt gagaaagtcg aagaagaggg cgagcgcgat gaggaagacg atgaaagccg
     1321 tcatggcgtc aagcctgctt gacgaggatg gactccacga tgtgcaccac gtcctcgcag
     1381 aggtccagca cctcctcggc gaaggcgtag atctccttcc acttcacgat gtgaatcggg
     1441 tcggtcgcgt tctcgaagag cctggccagg ctcgcgtcac gcatcgtgtc gccgacgttc
     1501 tcgaggcggt tcacctcgat gcacgcatcc ttggtcgcct ggagcatctt ggggtctttc
     1561 agcccctcga ccgccgtcac cagggtcttc accgatttct cgatgatcgt ggcgaattcc
     1621 acgagggtgt cgtccggggt gtcgatcctg tagacccgca tgcggttgct tatggtgtag
     1681 atcatgtcta tgacgtcgtc cagggcgcag gcgaggttgt ggatgtcctc gcggtcgaag
     1741 ggggttatga aggtcttgtt gaggcggtcc atgagatcgt gggcgacctc gtccgcctgg
     1801 tgctcgatat ctcgcatctg gttgaccgtc tccgcatcga acctcccgtc gcgggtctgc
     1861 cgggcgaaca gggtggcggc tctcacggcc agctccgcct gcttgttgaa caggacgaag
     1921 aagtcggtgg tcttgggaag caagctgaag cccatgccta ccctcctcct ggtcaagaag
     1981 ttgaacagat ggttatggtg tggaatcgat gcctggattt tcttatagag attgcgtcgc
     2041 ccagtcaagc cctgatcgga cgcgccctgc ctcccggcat cgcgcccggg atgcgcgtcg
     2101 aggccttcgc attcacgcgt cgcgtggctc gaggtcgggc agcgtcaccc tgaaggtcgt
     2161 gcccgagccc ggcatgctct ccacctctat ggcgcctccc aggatcgtcg cggcgttccg
     2221 ggcgatggac agcccgagcc ccgtgccgcc ggtgctcctc gagcgcgact tgtccaccac
     2281 atagaacctc tcgaagatcc tcctttggtg ttccctgggg ataccgatgc ccgtgtccgt
     2341 cacctcgatc gtcgtcgtgc ggccttgctt gctcgccctc acggtgatgg agccccgttc
     2401 cgtgtacttc accgcgttgt cgacgaggtt gatgaggatc tggtccacca ggaaggggtc
     2461 ggtcct
//
LOCUS       DAOSCR010000426         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27796_length_2463_cov_3.289867, whole genome shotgun sequence.
ACCESSION   DAOSCR010000426 DAOSCR010000000
VERSION     DAOSCR010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27796_length_2463_cov_3.289867"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLT69_09885"
     CDS             complement(<1..618)
                     /locus_tag="PLT69_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ14789.1"
                     /translation="MGGLFGVASRTDCVRDLFYGTDYHSHLGTRRAGLAVVNEQGFTR
                     SIHSIESSYFRTKFEPDLAIFKGAMGIGVISDTDSQPLIINSHLGTFGIVTVGRINNM
                     AELGRKAFSQRKYFSETSGYDINPTELVAMLICEQDNFVDGIRHVFENIRGSCTLLVL
                     AKEGIYAARDRYGRTPLVIGKRDGAYAVSSESSAFPNLGFETDMFL"
     gene            complement(918..2456)
                     /gene="guaA"
                     /locus_tag="PLT69_09890"
     CDS             complement(918..2456)
                     /gene="guaA"
                     /locus_tag="PLT69_09890"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOJ14790.1"
                     /translation="MREFVAVLDYGSQYAQLISRRIRECGVYCEILPHDLKAPELDSM
                     HPGAVVLSGGPASVLDKGSPGIDPEVFELGVPILGICYGLQLTSKVLGGVIERGRSGE
                     YGPALIEIDVPGRLFDGLGERLDVWMSHGDHVSVPPEGFTVLAHTDTCQVAAMADESR
                     KIYGVQFHPEVAHTPLGKEILRNFLYKIAGLSGGWSMKGFIEESVRSIREQVGDSKVI
                     CGMSGGVDSSVVAALLDKAVGPRMTAIFVDNGLLRASEAQEIRRTFTETYPLNLDIVD
                     ARKRFLDALRGVTDPEEKRKVIGKTFIEVFYNEADRIGGAEFLAQGTLYPDVIESRSA
                     KGGPSATIKSHHNVGGLPPDLKFKLIEPLKELFKDEVRLLGRELGLPETIIERQPFPG
                     PGLAVRILGEVTAENVEILQEADKRVQEEMAAWEGYRGVWQSFAVLLPVKSVGVMGDE
                     RTYANVIAIRAVHSVDGMTADWVKVPYELLARMSTRIINEVRGVNRVVYDISSKPPST
                     IEWE"
BASE COUNT          407 a          816 c          764 g          476 t
ORIGIN      
        1 gaggaacatg tccgtctcga acccgagatt gggaaacgcg ctcgactcgg atgacacggc
       61 gtaggcgccg tcccttttcc cgatgacgag aggggtcctg ccgtagcggt cacgggccgc
      121 gtaaatgccc tccttcgcga gcaccaagag cgtgcaggaa ccccgaatgt tctcgaacac
      181 gtgccgtatg ccgtcgacga agttgtcctg ttcacagatg agcatggcca cgagctcggt
      241 ggggttgatg tcgtaacccg aggtctcgga gaagtacttc ctctgcgaga atgcctttct
      301 gccgagctcc gccatgttgt tgatcctgcc cacggtcacg atgccgaagg tgccgaggtg
      361 agagttgatg atgaggggct gggaatcggt gtcgctgatg acgccgatgc ccatggcgcc
      421 cttgaagatc gccaggtccg gctcgaactt ggtcctgaag tacgaactct cgatggagtg
      481 gatggacctc gtgaaaccct gttcgttgac gacggcgagg cccgccctcc tggtgccgag
      541 gtgggaatgg tagtcagtgc cgtagaagag gtccctcacg caatcggtcc tggaagcgac
      601 gccgaacaac ccgcccatgt cctgcccccc ttgctttcgt tgtggcgatt cctcgaggtt
      661 ctctttctta caaagtgtct tcgttcagtg caagcgagcc gcgatcggcc gtcgtgtccc
      721 gaggcgccca ggagacgggc tcctccgtgc gcccgggatt gcgtggagga cggccatgcc
      781 gatcgatcct tcccagcctc gccgcggctc gacacgcagg ccggtccccg ggaaccgttg
      841 catgcccttc gggcccgctt cccgtaacac ccctcaccgg gtgcaacccg acgtcgcgcc
      901 gcacgggcgg gcccgtctca ctcccactcg atggtgctcg gcggcttcga gctgatgtcg
      961 tagacgacgc ggttgacgcc cctgacctcg ttgatgatcc gggtggacat cctggccagc
     1021 agctcgtagg ggaccttcac ccagtcggcg gtcatgccgt ccacgctgtg aaccgcccgg
     1081 atggcgatca cgttcgcgta cgtcctctcg tcgcccatta cgcccacgct cttgaccggc
     1141 agcagcactg cgaaggactg ccagacgcca cggtagccct cccacgcggc catctcctcc
     1201 tgcaccctct tgtccgcctc ctgcaggatc tccacgttct cggcggtcac ctcgccgagg
     1261 atcctgaccg ccaggcccgg ccccgggaag ggttgccgct ctatgatggt ctcgggcagg
     1321 ccgagctccc tgccgagcag gcggacctcg tccttgaaga gttccttgag gggctcgatg
     1381 agcttgaact tgaggtcagg gggcagcccg cccacgttgt ggtggctctt gatggtggcc
     1441 gaaggcccac ccttggccga cctgctctcg atgacgtccg ggtacagggt gccctgggcg
     1501 agaaactcgg cgccgcctat gcgatccgcc tcattgtaga acacctcgat gaaggtcttt
     1561 cctatgacct tgcgtttctc ctccgggtcg gtcacccccc tgagcgcatc cagaaagcgt
     1621 tttctggcat cgacgatgtc caggttgagc gggtaggtct ccgtgaaggt ccgtcggatc
     1681 tcctgtgcct cagaggccct gaggaggccg ttgtccacga atatggcggt catgcgggga
     1741 cccaccgcct tgtcgagcag ggccgccacc accgacgagt ccaccccgcc cgacatgccg
     1801 cagatcacct tcgagtcccc cacctgttcc ctgatggagc gtaccgattc ctcgatgaac
     1861 cccttcatgg accacccgcc ggagagaccg gctatcttgt agaggaagtt ccggaggatc
     1921 tcctttccca ggggggtatg ggcgacctcg ggatggaact gcaccccgta gatcttcctc
     1981 gactcgtcag ccatggccgc gacctggcag gtgtccgtgt gggcgagcac cgtgaacccc
     2041 tcaggcggga cgctcacgtg gtcaccgtgg ctcatccaga cgtcgagtct ttcgccgagg
     2101 ccgtcgaaga gccttccggg gacgtcgatc tcgatgagag ccggcccgta ctcccctgat
     2161 cgtccacgtt cgatgacccc tcccaggacc ttcgaggtga gctgcaaccc gtagcagatg
     2221 ccgaggatgg gcacgccgag ctcgaagacc tcggggtcga tgcccgggct gcctttgtcg
     2281 agcaccgatg ccgggcctcc cgagaggacc accgcccccg ggtgcatgga gtcgagttcg
     2341 ggggccttca ggtcgtgggg caggatctcg cagtacacac cgcactcgcg gatgcgcctc
     2401 gatatgagct gggcgtactg cgaaccgtag tcgagcaccg cgacgaattc acgcatggct
     2461 cgt
//
LOCUS       DAOSCR010000427         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27848_length_2460_cov_3.943867, whole genome shotgun sequence.
ACCESSION   DAOSCR010000427 DAOSCR010000000
VERSION     DAOSCR010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27848_length_2460_cov_3.943867"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..835)
                     /locus_tag="PLT69_09895"
     CDS             complement(44..835)
                     /locus_tag="PLT69_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOJ14791.1"
                     /translation="MRKFVVCCIVAMMLAGCAGRLPVKEEADPGRQAMRQAVSDLRAG
                     LEALEDEDDDAAWAAFARAFRAFDEASRPSDEPLKDASVKLVKVVETGGFEYARRQAL
                     SLLLLYIDMQKAYADRDGRSADELLQRFDSVYRETVQVTLQEKQRRMEELNAEIASRK
                     EELLKTSEPSLLETFPSSYTVRKGDTLPGIASRREIYNDSFMWPLIYKANRDQIKDPK
                     VLYEGQDLKIPREMAVEEIVEARREAGAPEPEKIPKDAYTPRRKK"
     gene            complement(835..>2460)
                     /gene="aspS"
                     /locus_tag="PLT69_09900"
     CDS             complement(835..>2460)
                     /gene="aspS"
                     /locus_tag="PLT69_09900"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOJ14792.1"
                     /translation="QVVCNPQDEPKAHAVARTLKQEYCISVTGTLSRRPEEMRNPAMA
                     TGEVEVLASGIEVFNEAETPPFVIDDSAEVTDTLRLRYRYLDLRRPSLQRNLFLRHQA
                     ASSTRRFLDEHGFVEVETPVLTKSTPEGARDYLVPSRIFPGKVFALPQSPQLFKQLLM
                     VSGFDRYYQIVRCFRDEDLRADRQPEFTQIDIEMSFVAMEDVLSLTEEMIAVMFRDTI
                     GVELARPFPRLTYDQAMETYGTDRPDMRFGMRLVDVTDIAATSEFRVFRQTAENGGVV
                     KAIAVKAPHDLSRKDLDSLHQAVAEHGAKGVLWARLEEDGWKSTLAKFFSDAQWAGIM
                     MRLEMAPGDVALFVADRREVVDASLSALRLHLAQRLGLVQKEAYAPLWVTGFPLFEYS
                     EEEQRLVSVHHPFTAPLPEDEALLGAEPERVRAQAYDMVINGYEVGGGSIRIHKRDIQ
                     QKVFGVLGLSDEQAKTKFGFLLEALGYGAPPHGGIAFGFDRLVMLLSGASSIRDVIAF
                     PKTQKAACLMTEAPSDADPAQLAQLGLKLAEEA"
BASE COUNT          379 a          849 c          749 g          483 t
ORIGIN      
        1 cgtgtagtag aacaccctct tgtgcggatc ccaccttgcc atgctatttc ttcctcctcg
       61 gcgtgtacgc gtccttgggg atcttctcgg gctcgggcgc accggcctcc ctcctggcct
      121 cgacgatctc ctcgaccgcc atctccctcg ggatcttgag atcctgccct tcgtagagca
      181 ccttcgggtc cttgatctgg tccctgttcg ccttgtagat gagcggccac atgaaggagt
      241 cgttgtagat ctcccgcctg gaggcgatcc ccggcagcgt gtcacccttg cgcaccgtgt
      301 aggaggaagg gaaggtctcg agcagcgagg gttctgaggt cttcaacagt tcctccttgc
      361 gcgaggctat ctcggcgttc agttcctcca tgcggcgctg cttctcctgg agggtgacct
      421 gcacggtctc gcggtagacg gagtcgaacc tctgcagaag ctcgtctgcg gaccggccgt
      481 ccctgtcggc ataggccttc tgcatgtcga tgtacagcag gaggagggaa agggcttgac
      541 gccttgcata ctcgaacccg cccgtctcca ccaccttgac gagcttgacg gaggcgtcct
      601 tcaggggctc gtccgagggt ctcgacgcct cgtcgaacgc cctgaaggcc ctggcgaacg
      661 cagcccacgc cgcgtcgtcg tcttcgtcct ccagggcctc gaggccggcc ctcagatcgg
      721 agacggcctg tctcatcgcc tgcctgcccg ggtcggcctc ctccttcacg ggaaggcgtc
      781 ccgcacagcc cgcgagcatc atcgccacga tgcagcagac cacgaacttt ctcatcacgc
      841 ctcctcggcg agtttcagcc ccagctgggc gagctgggcg gggtcggcgt ccgaaggggc
      901 ttccgtcatg aggcaggccg ccttctgggt cttggggaag gctatcacgt cccttatgct
      961 cgatgccccg gagagcagca tcacgagcct gtcgaacccg aaggctatgc cgccgtgggg
     1021 aggcgcgccg tagccgagcg cctccaggag aaacccgaac ttggtctttg cctgctcatc
     1081 gctcagcccg agcaccccga acaccttctg ctggatgtcc ctcttgtgaa tccttatgct
     1141 cccgccgccg acctcgtaac cgttgatgac catgtcgtac gcctgtgccc tgaccctttc
     1201 gggttcggca ccgagcaacg cctcgtcctc gggcagcggc gccgtgaacg ggtggtggac
     1261 cgacacgagc ctctgttcct cctcgctgta ctcgaagagg gggaagccgg tgacccacag
     1321 cggcgcgtac gcctccttct gcaccagccc gagcctctgg gcgaggtgga gcctgagcgc
     1381 cgagaggctc gcgtcgacca cctccctcct gtccgcgacg aagagcgcca cgtcgccggg
     1441 cgccatctcg agcctcatca tgataccggc ccactgtgcg tcgctgaaga acttggccag
     1501 cgttgacttc cacccgtcct cttcgaggcg ggcccagaga acacccttcg ccccgtgctc
     1561 cgccacggcc tggtgcagcg aatcgaggtc cttcctgctc aagtcgtggg gggccttgac
     1621 ggcgatggcc ttcaccacgc ccccgttctc cgcggtctgc ctgaacaccc tgaactcgga
     1681 tgtggcggcg atgtccgtca cgtcgacgag cctcatcccg aaacgcatgt ccggcctgtc
     1741 tgtgccgtac gtctccatgg cctggtcgta cgtgagcctg gggaagggcc tggcgagctc
     1801 cacgccgatg gtatcccgga acatgacggc gatcatctcc tcggtgagcg aaaggacatc
     1861 ctccatggcc acgaaagaca tctcgatgtc tatctgggtg aactcgggct gcctgtccgc
     1921 ccggagatcc tcgtcgcgga agcacctcac gatctggtag tacctgtcga aaccgctcac
     1981 catgaggagc tgcttgaaca actggggcga ctgcgggagg gcgaagacct ttcccgggaa
     2041 gatcctgctc ggcacgaggt agtcccgcgc cccttcgggc gtgctcttgg tgaggacagg
     2101 ggtctccacc tccacgaaac cgtgctcgtc gaggaagcgt ctcgtggagg acgccgcctg
     2161 gtgcctgagg aacaggttgc gctggagcga cggcctcctg aggtccaggt agcggtaccg
     2221 cagcctgagc gtgtccgtca cctcggcgga gtcgtcgatg acgaacggcg gcgtctccgc
     2281 ctcgttgaag acctctatcc cgctcgccag cacctctacc tcgccggtcg ccatggcggg
     2341 gttgcgcatc tcctcgggcc tcctcgacag cgtgcccgtc accgagatgc agtactcctg
     2401 cttgagggtc ctcgccaccg cgtgcgcctt gggctcgtcc tgcgggttgc agaccacctg
//
LOCUS       DAOSCR010000428         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27850_length_2460_cov_3.241580, whole genome shotgun sequence.
ACCESSION   DAOSCR010000428 DAOSCR010000000
VERSION     DAOSCR010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27850_length_2460_cov_3.241580"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLT69_09905"
     CDS             complement(<1..692)
                     /locus_tag="PLT69_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOJ14793.1"
                     /translation="MASGIRDKVAILGMGCTRFGERWNDSAEDLLVEAFQECIEDAGI
                     DKGEIQAAWLGVCFDEVNIGKSALAASTALRLPNIAVTRVENFCASGTESFRGAVYAV
                     ASGACDIALAMGVEKLKDIGYGGLPEFSTALGITNSMWFPNMTAPGGFAMLATGYAAK
                     YGLDIQEVKKAMAHVSVKSHANGALNPKAHLRRPVTEEQVLMAPMIAFPLGLFDCCGV
                     SDGAAAAIVVRA"
     gene            complement(704..2161)
                     /locus_tag="PLT69_09910"
     CDS             complement(704..2161)
                     /locus_tag="PLT69_09910"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOJ14794.1"
                     /translation="MVGIQAYGGYVPRYRISRMTIIQNMAWYFPVIMAVAGGEKAIAN
                     WDEDAVSMAVEAAYDCLAARDRKRVDGLFLASTTLPFADRLNAGIVAAALNLVEDGLS
                     AADYTACQKAGTTACLAALDAVSSGSKSGVLVVASDQRRTKMATMFEMFYGDGAAALL
                     FGRDDVIAEFIGAYSMNCDFVDHYKGYGKEFDYGWEERWVRDEGYGRIIPQAVNAFLA
                     KHSMKIADFAKVVYPCYFTGTHRAIAKELGIDQSHLQDNLHNTCGDTGAAHPILMLAQ
                     ALEGAKPGDRILVAGFGQGCDVLAFAVTENITKFRPAQGVTASLASRVEFTNYLKFTK
                     FRDLITADLGIRGEANPNASLTVLWRKRKMILGLTGHLCTKCGTPQFPPMPMCVNPNC
                     QAVGSFEDYEFAGRRGRVEMFTGDLLSPSVDPPAVYGLVSFDGGGKIFVDFTDCDLNQ
                     VKVGMPVKMAFRRRTRDQMRGFTGYFWKAVPQVQG"
     gene            complement(2178..>2460)
                     /locus_tag="PLT69_09915"
     CDS             complement(2178..>2460)
                     /locus_tag="PLT69_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOJ14795.1"
                     /translation="VDACVEPVLGVREAFESPHAVARGIVVEVPGPGGRPIRQVACPV
                     KFSGFEPKYLRCGPALGQDTQEVLASLGYTPGQVEDMEAQGIVYTGATP"
BASE COUNT          402 a          864 c          745 g          449 t
ORIGIN      
        1 tccgcacgca ccacaattgc cgccgccgcc ccatcgctca cgccgcagca gtcgaacagg
       61 ccgagaggga aggcgatcat gggcgccatg agcacctgct cctcggtgac aggcctcctc
      121 aggtgcgcct tgggattcag ggcgccgttg gcatggctct tcaccgagac gtgggccatg
      181 gccttcttca cctcctggat gtcgaggccg tacttggccg cgtacccggt ggccagcatg
      241 gcgaagccgc cgggcgccgt catgttgggg aaccacatgg agttcgtgat gccgagcgcc
      301 gtgctgaact cgggcagccc cccgtaccct atgtccttga gcttctccac gcccatggcg
      361 agggctatgt cgcatgcccc cgaggccacg gcgtagaccg cgcccctgaa cgactcggtg
      421 ccgctcgcgc agaagttctc cacccgggtc accgcgatgt tgggcaggcg cagcgccgtg
      481 gatgcggcaa gcgcgctctt gcctatgttg acctcgtcga agcacacacc cagccacgcc
      541 gcctggatct cccccttgtc gatgcccgcg tcctcgatgc actcctggaa cgcctccaca
      601 aggaggtcct cggcgctgtc gttccagcgc tctccgaaac gcgtgcaccc catgccgagg
      661 atggcgacct tgtctcttat cccgctagcc atgacccacc tcctcatccc tgtacctggg
      721 gcactgcctt ccagaagtac cccgtgaatc cgcgcatctg gtcgcgcgtc cgccttctga
      781 acgccatctt caccggcatg cccaccttta cctggttcag gtcgcagtcg gtgaagtcga
      841 cgaagatctt ccccccgccg tcgaacgaga cgagcccgta gaccgccggc gggtcgaccg
      901 atggcgagag caggtcgccc gtgaacatct cgaccctgcc cctcctcccg gcgaactcgt
      961 agtcctcgaa gctgcctaca gcctggcagt tcgggttgac gcacatgggc atgggcggga
     1021 actgaggcgt cccgcacttg gtgcacaggt gcccggtgag gccgaggatc atcttgcgct
     1081 tgcgccacag cacggtcaaa gacgcgttcg ggttggcctc gcccctgatg ccgaggtccg
     1141 ccgtgatgag gtcgcggaac ttggtgaact tgaggtagtt cgtgaactcg accctcgatg
     1201 ccaggcttgc ggtcacgcct tgggccggcc ggaatttcgt tatgttctcc gtgaccgcaa
     1261 acgcgagcac gtcgcagccc tggccgaagc cggcgaccag gatccggtcc ccgggcttgg
     1321 ctccctcgag cgcctgcgcc agcatgagga tggggtgggc ggcgcccgtg tccccgcagg
     1381 tgttgtggag attgtcctgg agatgggact ggtctatgcc caattccttg gcgatcgccc
     1441 tgtgcgtgcc cgtgaagtag cacgggtaga cgaccttcgc gaagtcggca atcttcatgg
     1501 agtgcttcgc gagaaaggcg ttgacggcct gggggatgat cctgccgtag ccctcgtcgc
     1561 ggacccagcg ctcttcccag ccgtagtcga attccttgcc gtagcccttg tagtgatcga
     1621 cgaaatcgca gttcatcgag taagcgccga tgaactcagc gatgacgtcg tccctgccga
     1681 acaacagggc ggccgcaccg tcgccgtaga acatctcgaa catggtggcc atcttggtgc
     1741 gcctctggtc cgaggccacg acgagcacgc ccgacttcga gcccgaggac accgcgtcga
     1801 gagcggcaag gcacgccgtg gtacccgcct tctggcaggc ggtatagtcc gctgctgaaa
     1861 gcccgtcttc cacgaggttg agcgcggccg cgacgatgcc cgcgttgagc ctgtccgcaa
     1921 agggcaaggt cgtcgacgcg agaaacaggc cgtcgacgcg cttcctgtcc cttgccgcga
     1981 ggcagtcgta agcggcctcc accgccatgc tgaccgcgtc ctcgtcccag ttcgcgatcg
     2041 ccttctcacc tccggccacc gccatgatca ccgggaaata ccatgccatg ttctggatga
     2101 tggtcatgcg gcttatccgg taacgcggca cgtacccccc gtacgcctgg atgcctacca
     2161 tacgcgaata ccccctttca tggagttgcc cccgtgtata cgatcccctg cgcttccatg
     2221 tcctcgacct ggccgggcgt gtaccccagc gatgcgagca cctcttgggt gtcctgtccc
     2281 agggccgggc cgcacctcag gtacttgggc tcgaatcccg agaacttcac cgggcacgcc
     2341 acctgccgga tcggcctgcc gccgggcccc ggcacctcga cgacgatccc cctggccaca
     2401 gcgtgcgggc tctcgaaggc ctcacggacg ccgagcaccg gctcgacgca cgcatccacg
//
LOCUS       DAOSCR010000429         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_27967_length_2453_cov_3.597581, whole genome shotgun sequence.
ACCESSION   DAOSCR010000429 DAOSCR010000000
VERSION     DAOSCR010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27967_length_2453_cov_3.597581"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="pabB"
                     /locus_tag="PLT69_09920"
     CDS             complement(<1..1403)
                     /gene="pabB"
                     /locus_tag="PLT69_09920"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOJ14796.1"
                     /translation="MRRAGESGAVAVNEAVVRDAYTGRWLHFHDPVAVVTVDDAGDVP
                     EGLALMEREVETRGLNAVCMISYEASSGFDPLLPVHGDDAFPALWFALYRDPGDAQGL
                     FSVPLRPCTGMSLRPEISKEAYGEAFGLIRKHLEQGDVYQVNYTFRLRGAFEDDPWRF
                     FAMFAPGHGDYGAFVLTEDWAVLSASPELFFSLEGDLLESRPMKGTAPRDKDPARDVS
                     AAGALLSSPKERAENLMIVDMVRNDMGKVARPGSVEVPALFTLERYPTVWQLTSTVRA
                     RTRSPLRDILAALFPPASITGAPKKRSMEIIRVAERSRRRIYTGAIGWCGPGRKARFS
                     VAIRTLLVDRRLGQAEYGVGGGVVWDSSMEAEWSECMAKSLVLADAARPFKLLETMLW
                     VPGRGYGLLDHHLARLAASAEHFGFPQDPLRLRERLETLASSFGDTPMRVRLLVGEDG
                     DVETETARFTPASKETP"
     gene            complement(1416..1997)
                     /gene="pdxT"
                     /locus_tag="PLT69_09925"
     CDS             complement(1416..1997)
                     /gene="pdxT"
                     /locus_tag="PLT69_09925"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF013350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOJ14797.1"
                     /translation="MVIGILALQGDYAEHRSCLEKTALGCRLVRTPADLDGIAGLVIP
                     GGESTAIKRLVASSGLVEPVMDLIERCIPVWGTCAGVVLLAKGGPWDVAGVRVERNAY
                     GPQVYSRVVMARTSIAEGDVPLVFIRAPRIVDAPRDASVLCRLGDEIVALRWKNLLLT
                     TFHPELAGGEPFTSYFASMVASPPRRGGSMEGA"
     gene            complement(1997..>2453)
                     /locus_tag="PLT69_09930"
     CDS             complement(1997..>2453)
                     /locus_tag="PLT69_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOJ14798.1"
                     /translation="SMIRTKGEAGTGDVVEAVRHMRAVQRDLDRLLGMSDEEVPAYAE
                     TIGSPLDTLIEIRRQGRLPVVNFAAGGIASPADAALMVHLGAQGVFVGSGIFTSENPP
                     RLAHAIVEAVAHADDPAWVARVSSGLGALMHGIDVRGMPDKDRLALRGR"
BASE COUNT          398 a          894 c          790 g          371 t
ORIGIN      
        1 tcgggggttt ccttgctcgc gggcgtgaac cgagccgtct ccgtctccac gtccccgtct
       61 tcgccgacga gcagcctcac ccgcatcggg gtgtcaccga aggacgaggc cagggtctcg
      121 agccgttcac ggagccggag gggatcctgc gggaagccga agtgctcggc ggacgccgcg
      181 aggcgggcga ggtggtggtc caggagcccg tagcctcggc cgggtaccca cagcatggtc
      241 tccaggagct tgaacgggcg cgccgcatca gcgagcacca gggatttggc catgcactcc
      301 gaccactccg cctccatcga cgagtcccac acgacacctc cgcccacgcc gtactccgcc
      361 tgcccgaggc gcctgtctac gaggagggtc ctgatcgcca cgctgaacct ggcctttctc
      421 ccgggaccac accagccaat ggcgccggtg tagatacgcc tgcgggacct ctccgccacg
      481 cggatgatct ccatcgacct cttcttgggg gccccggtga tggacgcggg gggaaagagc
      541 gcggcgagga tgtcgcggag cggggaacgc gtgcgcgccc tgaccgtgga ggtgagctgc
      601 cagaccgtgg ggtagcgctc gagggtgaac agggccggga cctcgacgct cccgggccgc
      661 gccaccttgc ccatgtcgtt acggaccatg tccacaatca tcaggttctc ggcccgctcc
      721 ttcggcgagg agagcagcgc tcccgcggcc gatacatccc gggccgggtc tttgtccctc
      781 ggcgccgtcc ccttcatggg cctcgactcg aggaggtcgc cctcgagcga gaagaacagc
      841 tccggcgacg cgctcagaac ggcccagtcc tctgtcagca cgaacgcgcc gtaatcgccg
      901 tggccgggcg cgaacatcgc gaagaaccgc caggggtcgt cctcgaaggc gcccctcagc
      961 ctgaaggtgt agttcacctg gtagacgtcc ccctgttcga ggtgcttcct gatgaggccg
     1021 aaggcctctc cgtatgcttc tttcgagatc tccggccgga gggacatgcc ggtgcagggc
     1081 ctcagcggca cggagaagag tccctgcgca tccccggggt cccggtagag ggcgaaccag
     1141 agggccggga aggcgtcgtc cccgtgcacg ggcagaaggg ggtcgaaccc gctggacgcc
     1201 tcgtaggaga tcatgcagac ggcgttgagg ccccgcgttt ccacctcgcg ctccatgagg
     1261 gcgagcccct cggggacgtc cccggcatca tcgaccgtca ccacggccac cgggtcatgg
     1321 aagtgcagcc agcgccccgt gtacgcgtcc ctcacgaccg cctcgttcac tgcgaccgca
     1381 cccgattccc ccgcacgcct catcgtcggc ccccgtcacg ccccttccat cgaaccgccc
     1441 cggcgaggcg gggaggccac catggacgcg aagtacgagg tgaacggctc accacccgcg
     1501 agctccgggt ggaaggtcgt cagcaggagg ttcttccacc tgagcgccac gatctcgtcc
     1561 ccgagcctgc agagcacgga ggcatcccta ggcgcgtcca cgatcctcgg cgccctgatg
     1621 aacacgaggg ggacatcccc ctcggcgatc gacgtcctcg ccatcaccac gcggctgtag
     1681 acctgcggcc cgtaggcgtt ccgctccacg cgcacaccgg cgacgtccca gggcccaccc
     1741 ttggccagga gcacgacgcc ggcgcacgtg ccccagacag ggatgcagcg ctcgatgaga
     1801 tccatgaccg gttccacgag gcccgacgag gcgacgagcc tcttgatggc cgtgctctcg
     1861 ccgcccggga tcacgagccc ggcgatgccg tcgaggtctg caggggtacg taccagcctg
     1921 cacccgagcg ccgtcttctc gaggcatgag cgatgctccg cgtagtcccc ctgcagagcg
     1981 agaatgccta tcaccatcac cgccccctga gtgcgagcct gtccttgtcc ggcatccccc
     2041 tcacgtcgat gccgtgcatg agggcgccca gccccgaaga aacccgcgcc acccaggcgg
     2101 ggtcgtccgc gtgcgccacc gcctccacga tggcatgcgc gaggcgagga gggttttccg
     2161 aggtgaagat gcccgagccg acaaacaccc cctgggcccc caggtgcacc atgagggcag
     2221 cgtccgcggg gctcgcgata ccgccggctg caaaattgac cacggggagc cgcccttgtc
     2281 gccgtatctc gatgagggtg tcgagcggcg agcctatggt ctcggcgtat gcgggaactt
     2341 cctcgtcgct catgccgagg agccgatcga gatccctctg caccgccctc atgtgtcgca
     2401 ccgcctccac cacgtcgccc gtcccggcct cacccttcgt cctgatcatg ctg
//
LOCUS       DAOSCR010000430         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28263_length_2437_cov_2.680521, whole genome shotgun sequence.
ACCESSION   DAOSCR010000430 DAOSCR010000000
VERSION     DAOSCR010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28263_length_2437_cov_2.680521"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..1217)
                     /locus_tag="PLT69_09935"
     CDS             complement(444..1217)
                     /locus_tag="PLT69_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14799.1"
                     /translation="MERTRLEKTTGSPTRVTFLGSGDAFGSGGRLPSAVHVGDGERGF
                     LVDCGPAVLPAMRRSGVEPSTVELILISHLHGDHLGGVPFFLLEEQLASARQAPLVIA
                     GPEGVGRVVSTLAETLFPGFSTSIVTFPLSFVELRPLVTVELCGVGILPFPAIHSAAS
                     NPLSLRVETGSRVIAYSGDTEWHDGLIEASRGADLFICECFSYHGPKANHLDHSTLVS
                     KKGLIDAKRIVIVHMDETMLARTGSLAFEPAFDGMVIEL"
     gene            complement(1447..2100)
                     /gene="nth"
                     /locus_tag="PLT69_09940"
     CDS             complement(1447..2100)
                     /gene="nth"
                     /locus_tag="PLT69_09940"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ14800.1"
                     /translation="MTNHEIDHVFEILSSVVNDLKEPIVGRVARDNDPFKVLVSTMLS
                     LRTKDETTEKACTRLFSLAKTPGEMIALPVAAIEDAIYPVGFYKTKAKNIVETSRIIV
                     RDHGGRVPSTLEDLLRLPGVGRKTANLVVTLGYDKPGICVDTHVHRITNRWGYVSTKT
                     PEQTEMRLREILPVRYWKDINSFLVPYGQYICRPTSPLCSRCRIFPLCDRVGVRVSR"
BASE COUNT          423 a          762 c          822 g          430 t
ORIGIN      
        1 ctaagcgatg aggccgagac agtcaacggc aaacaacccc ctgtgcaggt gtgcatgaag
       61 acggcaaggg gtgccggcat cgagacccca tcaggaaagc gggaaggtct cgtgcgggaa
      121 ggcggcctct cgccggcagc ggccccagtg gatccagcgg cgactgtctc aggcgtgttt
      181 ccccttcccg ggaaaggcat ccatgggtgc ggggcgcgag aacaaagcga aggccggccc
      241 tccttgaacg catgagacgg tcggcgcacg agccggtctc ccgccccggc gcacttggag
      301 cacgcaagcc gcggcgcacg gccgagcccg caggctcacg ccccggtctc agggcatgga
      361 gaccacgcaa acggcgtgga ggcgctttga cctttgggca cggcttgggc cataggcgcc
      421 ttggaccagg aaaggcggcg acgtcagagc tcgatgacca tgccgtcgaa ggcgggctcg
      481 aacgcgaggc tgcccgtacg ggcgagcatg gtctcgtcca tgtgcacgat cacgatcctc
      541 ttcgcatcga tgaggccctt tttcgagacg agggtggagt ggtcgaggtg gttggccttc
      601 gggccgtggt aggagaaaca ctcacagatg aagaggtccg ccccgcgtga tgcctcgatg
      661 aggccgtcgt gccactcggt gtcgcccgag tacgcgatga cccgcgatcc tgtctccacc
      721 ctcagcgaga gggggttgct ggcggccgag tggatcgcgg gaaaggggag gatccccacg
      781 ccgcagagtt cgaccgtgac gagcggcctc agttcgacga aggacagggg aaacgtgacg
      841 atggaggtgc tgaacccggg aaagagcgtc tccgcgagcg tcgagacgac cctgccgaca
      901 ccttcggggc cggctatgac gagaggggcc tggcgggccg aggcgagctg ttcctcgagg
      961 aggaagaagg gcacgccccc caggtggtcg ccgtggaggt ggcttatgag gatgagttcg
     1021 accgtggacg gctcgacgcc cgatcgtctc atggcaggca gcaccgctgg gccgcagtcg
     1081 acgaggaagc cccgttcgcc gtctcccacg tgcaccgcgc tcggcagtct ccccccgctc
     1141 ccgaaggcat cgccgctacc gagaaaggtc accctcgtgg gggatcccgt cgttttctcg
     1201 agacgcgttc tctccatgga gatcgcctcc tcctctctca tgtattcccc cgtccggcca
     1261 aggccgcgca ccgctctttt gaagaaggcg tcttgagcac gccgatggcg ccccgtcacc
     1321 taagccatgc ggcggcaacc gcagggacgc gcggtgtgcg gggccgcccc ggcctgtcct
     1381 gctcgccccc gcgcgggtgc gtaggggcca taggccgcct ctcatgcaat gcgccgtagg
     1441 acctcttcac cgggataccc tgacccccac ccggtcgcac agggggaaga tgcggcatct
     1501 cgagcacaac gggctcgtgg gacggcatat gtactggccg taggggacga ggaacgagtt
     1561 gatgtctttc cagtaccgca ccggcaggat ttccctgagc ctcatctcgg tctgttccgg
     1621 ggtcttcgtc gagacgtaac cccacctgtt cgttatccgg tggacgtggg tgtccacgca
     1681 tatgcccggt ttgtcgtagc cgagggtgac cacgaggttg gcggtcttcc tccccacgcc
     1741 cgggagtcgc aggagatcct cgagggtgga ggggacacgc ccgccgtgat cccgcacgat
     1801 gatcctcgag gtctccacga tgttcttggc cttggtcttg tagaacccca cgggatagat
     1861 cgcatcttcg atggcggcca cggggagggc gatcatctcc ccaggggtct tcgccagcga
     1921 gaagagcctg gtgcacgcct tctcggtggt ctcgtccttg gtcctgagcg agagcatggt
     1981 ggagacgagg accttgaagg ggtcattatc cctggccacc cttcccacga tcggctcttt
     2041 gaggtcgttg accacgctcg agaggatctc gaacacgtga tcgatctcgt ggttcgtcat
     2101 ggcatccttt catcacagcg cggcctgcaa ggcaagcacg aagggcgcct ccccggccgc
     2161 cacccaggcc cgcaggccat ggtcccgtat gtgcttgcac cgtcggcccg cctgtggttg
     2221 aatcgaggac gcaagcggca gggttcttgc aagatgttct ctcgaacgag cgtgttcgga
     2281 cggttgcgca cgtggttgtt tccagaaccg tggacaggag gaggtcatga tccggaaggt
     2341 gttccttgcg ttcgctgtcc tggcggcgct tgcctgtgca gggtgcagcg actcggaaga
     2401 gactgaaggg ctcggtttca tacccaagat caagctc
//
LOCUS       DAOSCR010000431         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28270_length_2436_cov_5.334733, whole genome shotgun sequence.
ACCESSION   DAOSCR010000431 DAOSCR010000000
VERSION     DAOSCR010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28270_length_2436_cov_5.334733"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT69_09945"
     CDS             <1..283
                     /locus_tag="PLT69_09945"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOJ14801.1"
                     /translation="CRDVDPSLFAQEEERSLHRAYESVRGAFLAKTRDGAYADALKKL
                     AGLKEPIDTFFDKVLVMSEDEKVRNNRIALLANLLDLFGAMASFSKIST"
     gene            336..1364
                     /locus_tag="PLT69_09950"
     CDS             336..1364
                     /locus_tag="PLT69_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ14802.1"
                     /translation="MKADMRDAFSIDVDRILHSQAYTSYIDKTQVFYLVGHQGISHRV
                     IHVQLLSRIARTIGRKLGLNDDLIEAIAIGHDIGHPPFGHDGETYLSELCTKANIGPF
                     RHNVQAIQALERIEKAGRGLNLTLQVLDGILCHNGEVTEQRLSPDGGVTFRDFDAKMR
                     AALAGASPLPMTMEGCLVRMADTVSYIGRDIEDAIAIGIIRRTDLPREVVKTLGDTNG
                     KIVYRLVEDLIGHSDGTSIGYSDEVFGALCRLKEFNYERIYTNKAVKRESDKIRNMYG
                     LVFARLVQDIEERERSSPVYTAFLDRLHERYLNTHNPFEMARDYVAGLTDSAFLRLFH
                     DLFVPRIA"
     gene            complement(1677..1886)
                     /locus_tag="PLT69_09955"
     CDS             complement(1677..1886)
                     /locus_tag="PLT69_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1845..1847),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HOJ14803.1"
                     /translation="MADNPVIIYGKAGUPYTSQARSAYKDKAVYYDVKQDPGRLEEML
                     KLSGGRRVVPVIVEGGHVKVGYGGT"
BASE COUNT          433 a          782 c          788 g          433 t
ORIGIN      
        1 gtgccgcgac gtggatcctt ccctgttcgc acaggaggag gaacgctctc tccaccgtgc
       61 atacgagtcc gtgcgcgggg cgttcctcgc aaaaacccgg gacggggcgt atgcagacgc
      121 actgaagaag ttggcgggcc tcaaggagcc gatagacacc ttcttcgaca aggtgctcgt
      181 gatgagcgag gacgagaagg tgcgcaacaa ccgtatcgcc ctgctcgcga acctcctcga
      241 cctcttcggt gccatggcct cgttctcgaa gatatcgacc tgagatggga cgatcccttc
      301 ccgcccaagg cgtcaggcgg aggcacgagg aggccgtgaa ggccgacatg agggacgcgt
      361 tctccatcga cgtggacagg atcctgcact cccaggccta cacctcctac atcgacaaga
      421 cccaggtctt ctatctcgtc gggcaccagg gcatctccca cagggtcatc cacgtgcagc
      481 tcctttcgcg gatagcgcga accatcggca ggaagctcgg gctcaacgac gacctcatcg
      541 aggccatcgc catcggtcac gacataggcc accccccgtt cggccacgac ggcgagacct
      601 atctcagcga gctctgcacg aaggcgaaca tcgggccgtt caggcacaac gttcaggcca
      661 tccaggccct cgaacgcata gaaaaggccg gccggggcct caacctgacc ctccaggtcc
      721 tcgacggcat cctctgccac aacggcgagg tcaccgagca gaggctctcg cccgacgggg
      781 gcgtgacgtt cagggacttc gacgcgaaga tgagggctgc cctcgcaggg gccagcccac
      841 tgcccatgac catggagggg tgcctggtcc gcatggcgga taccgtcagc tacattggga
      901 gggacatcga ggacgccatc gccatcggca tcatacgacg cacggatctc ccgagagagg
      961 tggtaaagac cctcggcgac acgaacggca agatcgtcta caggcttgtc gaggatctga
     1021 tcgggcactc ggacgggaca tccatcggct actcggacga ggtgttcggc gccctgtgcc
     1081 ggctcaagga gttcaactac gagaggatat acacgaacaa ggcggtaaag cgtgagtcgg
     1141 acaagatcag gaacatgtac ggcctcgtgt tcgcgcgcct cgtgcaggac atcgaggagc
     1201 gggagaggtc ctcgcccgtg tacaccgcct ttctcgaccg cctccatgaa aggtacctca
     1261 acacccacaa tccgttcgag atggccaggg attatgtggc gggactcacg gactccgcct
     1321 tcctgaggtt gttccacgac ctgttcgtgc cgaggatagc ctagcgggct cggctccggc
     1381 gccggtcgag ggacgccgct cgcggcccat gtgggcggat ccccagggcc tgacggcaac
     1441 cggccaacgt ccccatgatg cccgttttca acagatgagg aagcgcccgg tgtcgtcctg
     1501 ggtgtgagcg atgcggggag acgtgggagg gtgtgccaca ccccccggac ataccgttcg
     1561 tccgacccgt ccggggctca tgcccctcgt gggggcggac atggtgtgca cgcctcgggg
     1621 gaaacccctt ccgcgccttg ttgtgcgccc atcgcctgtg ggcgggccta cacccctcag
     1681 gtccccccgt agccgacctt tacgtggccg ccctcgacga tgacgggcac cacgcgcctt
     1741 ccgcccgaga gcttgagcat ttcctcgagc ctgccagggt cctgcttcac gtcgtagtag
     1801 accgccttgt ccttgtaggc cgacctggcc tgggatgtgt acggtcagcc ggccttgccg
     1861 tagatgatga cagggttgtc cgccatggga ggcctcctgg acctgtgtct ctccgcgttc
     1921 ggctctagtc tatcagaatg aaacgccatg ttcatgcaga aaatgcatgg atgtgccggc
     1981 gccgcgggtg catggagggc ccaggcccgc ccctggggat gcgctcatgc gttgcatggt
     2041 cttcctgttc ccgctcgccg tgtcctcttg ggcgggaatg cgtgcgtggg cggccctttc
     2101 gggatcggga tacctcacgg ctgacggggg gtgccacagg ggcgtcttca cgagcgccgg
     2161 tcgttggcaa ggggtttgat cctgctgtac gtctgcacgg aggacgtgca cgcgaaagca
     2221 cacagtgaga tcagcaggac cggccctcgg ccgcgttgtc cttgtgcgcc ccgacaggag
     2281 agagggtcgt gcaagggcgt tttcgggggc tcacggcccg gccacggccc cctccagggc
     2341 aaggtcggcg cgttttcgat gctcatgggg gctcacgggt tcaggtccgc cgggtgtgtg
     2401 gtcctgcgcg cgtcttcgat ccgcatgggg gctcac
//
LOCUS       DAOSCR010000432         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28286_length_2435_cov_3.121849, whole genome shotgun sequence.
ACCESSION   DAOSCR010000432 DAOSCR010000000
VERSION     DAOSCR010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28286_length_2435_cov_3.121849"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLT69_09960"
     CDS             <1..190
                     /locus_tag="PLT69_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOJ14804.1"
                     /translation="QVSVVEIKGSQVKLGIKAPREVSVHRQEIYLKIQEENLRAAQVT
                     AEALGEAQALFLDRENEP"
     gene            241..480
                     /locus_tag="PLT69_09965"
     CDS             241..480
                     /locus_tag="PLT69_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14805.1"
                     /translation="MRFLVDLIASIILAPFKLARFVIVDVLVKGVLAGVVSLVRSILK
                     LLLHPITLLLVASGAAAYFYSSEEQKKKVKALIGL"
     gene            1319..>2435
                     /locus_tag="PLT69_09970"
     CDS             1319..>2435
                     /locus_tag="PLT69_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ14806.1"
                     /translation="MGGDVLGIDVGSTAAGVVQIDGHGRVVRSAYGFHRGNIAGTLEG
                     LISRFPLGDVGYVACTSSCPPTADAVRRSDDTLASLRAARHLHGDIGALLCVGAERFS
                     LHILDEKGRYRSTRTNTSCAAGTGSFLDQQARRLGLKDSAHLDELASGNKGAVPLIAS
                     RCAVFAKTDLVHAQQSGFSPMEIADGLCRGLAVNIAETLFHGVVPEGTIVFCGGVSKN
                     TSVKAHLESLIQRALVVDDLSHLYGALGAALMLLDELGRVESASVSSIRGIFTDPGPR
                     RSLHYAPLELKRSSYPDFKGLEHREFVLPDGSFEVPVEVDVYEDLGGRERLRVTLGLD
                     IGSTSTKSVILGDGRRVIAGFYTRTSGRPVDAVRAILG"
BASE COUNT          414 a          779 c          809 g          433 t
ORIGIN      
        1 ccaggtctcg gtcgtggaga tcaagggatc ccaggtcaaa ctcgggatca aggccccgag
       61 ggaggtatct gtacacaggc aggagatcta cctgaagatc caggaggaga acctgcgcgc
      121 agcccaggtg accgcggagg cgctgggcga ggctcaggcc ctcttcctcg acagggagaa
      181 cgagccatga cgggcccggc ccgcaccatg gcatgacaac agcgaaggga ggtggcgcac
      241 gtgcgatttc tcgtcgacct gatcgcttcg atcatccttg cgcctttcaa gctggcacgg
      301 ttcgtgatcg tggacgtact cgtcaagggc gtgctcgcag gggtggtgtc cctcgtccgt
      361 tccattctca agctcctcct gcacccgata accctgctcc tggtggcatc cggagcggcc
      421 gcatacttct actcgagcga ggagcagaag aagaaggtca aggccctgat cgggctgtga
      481 ccggcccgga ccacccaggg ccagtccgga gtctcccatg gcctcgggag cccctgttcc
      541 gcccctggtg gccatgtcag gccgaacatg ccctgcagct ttgtccccct tgcggactcc
      601 cccggcgtga aacccatgcg ccggggccag gcctcgtccc gcatcgattg tgatgccaga
      661 gtagacacct tcctccacgc atgaggcgtg caggggatcg ttcgccctcg caggtccgcc
      721 gctgaccacc ttcccccacg cacgaggcgt gcagccacgc gcgtgtcgag gggagagccg
      781 cgcccggagt gctggcggcc ctgtcaatgt ggccggccgc cgttttcggg caggcggttc
      841 ccccggttcg cggccttgtc gtggtaggag gtggtgcatc gaggcacgct cccgatctac
      901 acaaaccgtc acgatagaaa atgacgcccc gcacgttcgt ggacggccgt cgagcccggg
      961 ctggaggtgt cttcgccccc tcggcttgct gcagcggccg aagggcgacc ggcagcgatc
     1021 cttgcgctac ggtgggttca gccaggggcc ccggtgggtc ggccttctgc acacgcgagg
     1081 tctgtccggc ccatggcgag gctctactga gtctccatgc caggacgcga tggcttgcag
     1141 gctcgtcgcc cgtcacccct tgtccccgag gcaacaggcg gatcgtgcca tggactccag
     1201 gtgccaccgc ccggtcgtca cccgcctctt gatttccctc gagaaggggg tatatgggat
     1261 gtaatgtaaa agaaatgtaa aacaaccttg tggtgaatgt tcgaggagtg agggcaacat
     1321 gggtggagat gtccttggaa tcgatgtcgg gtccacggcc gcgggcgtcg tccagatcga
     1381 cggtcatggc cgcgtggtac ggagtgcgta cggtttccac cgtggcaaca tcgctggcac
     1441 cctcgaaggc ctgatctcgc gctttcccct gggagacgtc gggtacgtgg cgtgcacctc
     1501 ttcgtgcccg ccgaccgccg atgcggtgag acgctccgac gacaccctgg cctcgctgag
     1561 ggcggcacga caccttcacg gggatatcgg cgcgctcttg tgcgtggggg ccgagaggtt
     1621 ctctctccat atcttggacg aaaagggccg ctacaggtcg acgaggacga acacctcctg
     1681 cgccgcgggg acgggcagct tcctcgacca gcaggcccgg aggctgggcc tgaaggacag
     1741 cgcccatctc gacgagctcg catccggcaa caagggcgcc gtcccgctca tcgcgagcag
     1801 gtgcgccgtg ttcgcgaaga ccgatctcgt gcatgcacag cagagcggat tcagcccgat
     1861 ggagatagcg gacggcctct gcagggggct tgccgtgaac atagccgaga ccctgttcca
     1921 cggggttgtg cccgaaggga ccatcgtgtt ctgcggcggg gtgtcgaaaa acacatccgt
     1981 caaggcacac ctcgagtccc tgatacaacg tgcactcgtg gtcgacgacc tgtcgcacct
     2041 ctacggcgcg ctgggcgccg ccctcatgct cctcgacgag ttgggcaggg tcgagtccgc
     2101 ttcggtctcg tcgatccgcg gcatcttcac ggatcccggc ccgaggagga gtctccacta
     2161 cgcccccctg gaactcaaac gttcctccta cccggatttc aaaggcctgg agcaccggga
     2221 gttcgttctc cccgacgggt ctttcgaggt accggtggag gtggatgtct acgaggatct
     2281 cggggggcgg gagaggctga gggtcacact cgggctggac atcgggtcca cgagcaccaa
     2341 gtcggtgatc ctgggcgacg ggaggagggt gattgccggg ttctacacga ggacctcggg
     2401 caggcccgtg gacgccgtcc gcgcgatact gggtg
//
LOCUS       DAOSCR010000433         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28475_length_2423_cov_3.244088, whole genome shotgun sequence.
ACCESSION   DAOSCR010000433 DAOSCR010000000
VERSION     DAOSCR010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28475_length_2423_cov_3.244088"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /locus_tag="PLT69_09975"
     CDS             <1..2120
                     /locus_tag="PLT69_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOJ14807.1"
                     /translation="AGGDPATLMTVGATTLADINRELVDKGLADWGRLEETVDEHEEA
                     PGGIVPSSRRQYLAEVARAVRSYHETTLETAEKIRTLAAYEKIAERNPDEKTLSSLKK
                     GMAELRRQIPSSERALVETWPEKAARYRSKTFSYAVRDKKIQVQLSRESLAGLMIPKV
                     ALPSFKDKGDLYTWLRKENVPGAFPFTAGVFPFKRNWEDPKRQFAGEGSPERTNRRFH
                     YLCRNDDAKRLSTAFDSVTLYGEDPQERPDIFGKVGESGVSVCTLDDMKKLYAGFDLC
                     SPDTSVSMTINGPAPIMTAMFFNTAIDQQVERFEKERGRKPGPEEREEIVARVLRTVR
                     GTVQADILKEDQGQNTCIFSTEFALKMMGDIQEYFIEHAVENFYSVSISGYHIAEAGA
                     NPITQLAFTLANAFTYVEYYLSRGMKVDDFAKNFSFFFSSGMDPEYSVLGRVARRIWA
                     IAMRDVYGADESSQRLKYHIQTSGRSLHSMEIQFNDIRTTLQALTALNDNCNSLHTNA
                     YDEAITTPTEESVRRAMAIQMIILREFGMSMNENPLQGSFFMEWLTDAVEDAVLREFD
                     RLSERGGVLGAMEMQYQRGKIQDESLHYEHLKHSGALPIIGVNTFENPDAHEQAGGAV
                     GGCCIPLTRASFEEKRAQLDNCRKFQKRHARKASAALMRLKKTALEGGNIFAELMDTV
                     RVASLGQITQALYEVGGRYRRNM"
BASE COUNT          492 a          751 c          826 g          354 t
ORIGIN      
        1 tcgccggtgg cgacccggcg acgctgatga ccgtcggcgc cacgacgctg gccgacatca
       61 accgggagct ggtggacaag gggcttgcag actgggggcg cctggaggag acggtcgacg
      121 agcacgagga ggccccgggc ggcatcgtgc cgtcgtcgag gaggcagtac ctcgcggagg
      181 tcgccagggc cgtccggtcg taccacgaga ccaccctgga gacggcggag aagatccgca
      241 cgcttgccgc ctacgagaag atcgcggagc gaaacccgga cgagaagacc ctctcgtcgc
      301 tgaagaaggg catggcggag ctcaggaggc agatcccatc atcagaacgc gccctcgtcg
      361 agacgtggcc cgagaaggcc gccaggtaca ggtcgaagac gttctcgtat gccgtgaggg
      421 acaagaagat ccaggtgcag ctcagcaggg agtcccttgc gggcctcatg atcccgaagg
      481 tggcgctgcc ttccttcaag gacaaggggg atctctacac ctggctcagg aaggagaacg
      541 tccccggcgc ctttccgttc accgcgggcg tcttcccgtt caagaggaac tgggaggacc
      601 cgaagaggca gttcgcgggc gaagggtctc ccgagagaac gaacaggcgt ttccactatc
      661 tgtgcaggaa cgacgacgcc aagaggctca gcacggcctt cgacagcgtg acgctctacg
      721 gcgaggaccc gcaggagcgc cccgacatct tcggcaaggt gggcgagagc ggggtgagcg
      781 tgtgcacctt ggacgacatg aagaagctct acgcggggtt cgacctctgc tccccggaca
      841 cgagcgtgtc catgaccatc aacgggcccg cgcccatcat gaccgccatg ttcttcaaca
      901 cggccatcga ccagcaggtg gagcgcttcg agaaagagcg cggcaggaag cccggtcccg
      961 aagagcggga agagatcgtc gcgagggtgc tcaggaccgt gaggggcacc gtgcaggctg
     1021 acatcctgaa ggaggatcaa ggtcagaaca cgtgcatctt ctccacggag ttcgccctca
     1081 agatgatggg cgacatccag gaatacttca tcgagcacgc cgtggagaac ttctactccg
     1141 tcagcatcag cggctaccac atagcggagg ccggggcgaa ccccatcacg cagctcgcct
     1201 tcaccctcgc gaacgccttc acctacgtcg agtactacct ctcgcggggc atgaaggtgg
     1261 acgatttcgc gaagaacttc tcgttcttct tctcgagcgg catggaccct gaatacagcg
     1321 tgctcggcag ggttgcacgc aggatctggg ccatagccat gcgcgacgtc tacggtgcgg
     1381 acgagtcgtc ccagcgcctc aagtatcaca tccagacctc gggccgctcg ttgcactcca
     1441 tggagatcca gttcaacgac atccgcacga ccctacaggc gctcacggcc ctgaacgaca
     1501 actgcaactc gctccacacg aacgcctacg acgaggccat caccacgccg accgaggagt
     1561 ccgtccgcag ggcgatggcg atccagatga tcatcctgag ggaattcggg atgagcatga
     1621 acgagaaccc cctgcagggg tcgttcttca tggagtggct cacggacgcg gtggaagacg
     1681 cggtcctgcg cgagttcgac cgcctgagcg agcgcggagg ggtccttggc gccatggaga
     1741 tgcagtatca gcggggcaag atccaggacg aatcgctgca ctacgagcat ctcaagcact
     1801 ccggggccct ccccatcatc ggggtgaaca ccttcgagaa cccggacgcg cacgagcagg
     1861 caggcggcgc cgtcggcggc tgctgcatac ccctcaccag ggcgtctttc gaggagaagc
     1921 gggcccagct cgacaactgc aggaagttcc agaaacgcca tgcccgcaag gcctcggccg
     1981 cgctcatgcg cctcaagaag accgcgctcg agggcggcaa catattcgcc gagctcatgg
     2041 acacggtgag ggtcgcaagc ctcgggcaga tcacccaggc gctctacgag gtgggcggca
     2101 ggtaccgaag gaacatgtag gggacgatgc agcgagagag cggccttcgc cccttcggca
     2161 cccgagcccc cagggcggcg ctgcgtcgac gggcgaggcc cggcgccgtg ttgcaggccg
     2221 tttcgggccg gggtcatcgc cgtgcatgaa cggctggaaa tgggccgggg agatgcggtg
     2281 ggggagtgtc ccgggaggct gtggaaggtg gggggtcgcc ccgcaggacc ccgcccccgg
     2341 gggcatgggg gacaccgtgc agtggtcggc atgtgcgagg ggtgatcgcc ccgggcagtg
     2401 gcggatgatc ccgcttaagg ggg
//
LOCUS       DAOSCR010000434         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28652_length_2412_cov_3.852355, whole genome shotgun sequence.
ACCESSION   DAOSCR010000434 DAOSCR010000000
VERSION     DAOSCR010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28652_length_2412_cov_3.852355"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..906
                     /locus_tag="PLT69_09980"
     CDS             163..906
                     /locus_tag="PLT69_09980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ14808.1"
                     /translation="MALIRVGQTEFDARIAIFDKDGTLIDFTGTWLAIVDLLLLRMAD
                     HVTMSGRLREKVQEVLGVSLDRGEVDPGGALAMGTFAECYALLCHCLHQEGLRWDEAQ
                     RVVAALDEEVFQSEDRNRCVRPAQGAVEALARLKEKGLHVAVATNDKTRDALSDLLAI
                     GAGPYIDMVVGADAVANPKPASDMVDKICGMAGSPPATAVLVGDTVMDASLGRNAGVG
                     LVVGVLGIEDRRVLEEHVDVVIGSLDEIS"
     gene            complement(995..1567)
                     /locus_tag="PLT69_09985"
     CDS             complement(995..1567)
                     /locus_tag="PLT69_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HOJ14809.1"
                     /translation="MTDEGLLFTDGEKAAILEAALSGVESVLHGARASLPVQERGLPP
                     RLAAPRGVFVTILEAGRVRGCMGGCEPLFPLIDACRIYAEAAAHRDPRFTPLARGSHP
                     VPSVEVAVLGGVRPLAVTDGSLLGDGKVGLVLSRGFRREVFLPRALIGAPRDVDGLLA
                     FLGDMARIDADDPHGRVEWGVFDVVVVAGP"
     gene            1873..>2412
                     /locus_tag="PLT69_09990"
     CDS             1873..>2412
                     /locus_tag="PLT69_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOJ14810.1"
                     /translation="MEDPSPLIRQRIEKYEQLKREFNLVHFPNTFRKDTDIETFIALY
                     GTRDAEELAGVKTVHRLAGRIMASRKFGKASFIHFQDSTGRLQAFFEMAKVGERPYEL
                     FKKLDIGDIIGLWGAPFKTKTGELSVYVEGFELVTKSYRPLPEKWHGLRDVETRYRQR
                     YVDLIVSPEVRRTFVTRSRI"
BASE COUNT          464 a          786 c          806 g          356 t
ORIGIN      
        1 ggacaacaac ccgcgggtct ggagaaaaag tgcgtagtat atagcatgga tgtgtggtaa
       61 cgcatcaacg tcaaaggccg gggccgccgc ctccaggggg cgaccagcct cggccgggca
      121 agagaggaga cggatcatgg acgcgctcga gggaggagag ccatggcgtt gatacgggtg
      181 ggacagacag agttcgacgc gcgcatagcg atcttcgaca aggacgggac gctgatcgac
      241 ttcaccggga cctggctcgc gatcgtggac ctcctcctgc tgcgcatggc ggatcacgtg
      301 accatgagcg ggcggctcag ggagaaggtg caggaggtgc tgggcgtcag cctggacagg
      361 ggagaggtgg acccgggagg cgccctggcc atggggacct tcgccgagtg ctatgcgctc
      421 ctctgccact gcctccatca ggagggtctc cgctgggacg aggcccagcg ggtcgtggcc
      481 gcattggacg aggaggtgtt ccagtccgag gacaggaaca ggtgcgtgcg cccggcgcag
      541 ggcgcggtcg aggcgctcgc caggctcaag gaaaagggcc tgcacgtggc cgtcgccacc
      601 aacgacaaga ccagggacgc cctctcggac ctgctcgcca taggtgcggg cccgtacatc
      661 gacatggtcg tgggagccga cgctgtggcc aatcccaagc cggcatccga catggtggac
      721 aagatctgcg gcatggccgg cagcccgccc gccacggccg tgctcgtggg ggacacggtg
      781 atggacgcct cgctcgggag aaacgcgggc gtgggtctcg tcgtgggggt cctgggcatc
      841 gaggacaggc gcgtgctcga agagcacgtg gacgtggtga taggctccct ggacgagatc
      901 tcgtaaacgg cgtctccttc ccgacaaccc tgcgccacgc tcacggcggg gcatgccgcg
      961 gccctgaccg catgctcccc tcccggccgg gacctcacgg tccggccacg accacgacgt
     1021 cgaacacacc ccactcgacc cgaccatggg ggtcgtcggc atcgatcctt gccatgtcgc
     1081 ccaagaaggc gagcaggcca tccacgtctc gaggcgcgcc tataagggcc ctcggcagga
     1141 agacctccct gcgaaacccc ctcgagagga ccaaccccac cttgccgtcc ccgaggaggc
     1201 ttccgtcggt gacggccagg ggcctcacgc ctccgagcac ggccacctcc acgctcggta
     1261 cgggatgaga cccccttgca agcggggtga acctcgggtc cctgtgcgca gcggcctcgg
     1321 cgtatatcct gcaggcatcg atgaggggga acaggggttc gcagcctccc atgcaccccc
     1381 tgaccctgcc ggcttcaagg atcgtgacga acacgcccct tggcgcggcc agcctggggg
     1441 ggaggccacg ctcctgcacg ggcaaggatg ccctcgcccc gtggaggacc gactcgacac
     1501 ccgagagcgc ggcctcaagg atggcggcct tctcaccgtc ggtgaacaac agcccttcgt
     1561 cggtcatgtt cccacgcctc aatgcggccc tgccgatggg tccggtgcag gccgaatgca
     1621 tgccatatac cacagcgccc cttcgatggt aagggcacgg acggacccac ccccgctcat
     1681 tgccgggcgg gagcggcacg ggagacggcc cgcccagggc ccccaccctg aaaggacgat
     1741 aggtcgggga acacctcctt gtcctcatgc ccggggaaac gttcccgagg ccacccggcg
     1801 gtgcggtttg ttgactcgct gcgggaaatt atctatataa gcgcacaacc caaatcccgt
     1861 ggagacgctt tcgtggaaga cccaagccct ctcatacgcc agcgcataga gaagtacgaa
     1921 cagctcaagc gcgagttcaa cctggtccac ttccccaaca ccttccggaa ggacacggac
     1981 atagagacgt tcatcgcgct ctacggcacc agggacgccg aggaactcgc aggcgtcaag
     2041 accgtgcacc ggctcgccgg gaggatcatg gcgtcaagaa agttcggcaa ggcgtcgttc
     2101 atccacttcc aggactccac gggcaggctc caggccttct tcgagatggc caaggtcggc
     2161 gagcgtccct acgagctgtt caagaagctc gacatcggcg acatcatagg cctgtggggg
     2221 gcgcccttca agacaaagac cggggagctc tcggtgtacg tcgaggggtt cgagctcgtc
     2281 accaagtcct acaggccgct gccggagaag tggcacggcc tcagggacgt ggagacgagg
     2341 tacaggcagc gctacgtcga tctcatcgtc tcgccggagg tcaggcgcac gttcgtgacg
     2401 aggagcagga tc
//
LOCUS       DAOSCR010000435         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28678_length_2411_cov_2.951613, whole genome shotgun sequence.
ACCESSION   DAOSCR010000435 DAOSCR010000000
VERSION     DAOSCR010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28678_length_2411_cov_2.951613"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLT69_09995"
     CDS             complement(<1..316)
                     /locus_tag="PLT69_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOJ14811.1"
                     /translation="MGEGSARDIVTTAATLFFILDPLGNIPTFHAVLANVPVRARRRV
                     ILRELFIALFVLVVFLVAGVRILGYLGLTQPSLGIAGGIMLFVIALRMVFPQAGQQDD
                     AAI"
     gene            complement(309..1337)
                     /gene="arsN2"
                     /locus_tag="PLT69_10000"
     CDS             complement(309..1337)
                     /gene="arsN2"
                     /locus_tag="PLT69_10000"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HOJ14812.1"
                     /translation="MSRGPFPQASLRQAEASGQTARRKEALESLQALYREIDRLASRT
                     ASLLAGRLRCRRGCSSCCVDGITVFEVEASTIATNNKALLEAGSAHPQGACAFLDPEG
                     ACRIYPDRPYVCRTQGLPLRWMEQGPDGSLVEMRDICPVNEPLVDLTALDASLCWPIG
                     PVEERLACIQMSLSGRHPTRIGLRSLFGRTCPVSFGPCTKEDLAEVKSLLEAAGLDAR
                     DVTAGTMGGFVQGRSLTGTLVACAGVEPHGTSGLLRSVAVHPGFRGCGHGAGVVHEAE
                     RLADSLGVTRLYLLTTTASPFMERLGYTITARDAVPAEIRSSGQFTSLCPQSATCMTK
                     DLKGGKDG"
     gene            complement(1334..2191)
                     /locus_tag="PLT69_10005"
     CDS             complement(1334..2191)
                     /locus_tag="PLT69_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOJ14813.1"
                     /translation="MSGLFVVAVFVLLILVTLFLGVRIVPQGSKHVVQRLGKYHTTLG
                     PGLNLIIPYIDTVAYKVTTKDIVLDIPSQEVITKDNAVIVVNAVAYINIISPEKAVYG
                     VEDYRVAIQNLVQTSLRSIIGEMNLDDALSSREKIKARLKESISDDIADWGIMLKTVE
                     IQDISPSDTMQKAMEEQAAAERARRATVTRAEGEKAAAILQAEGRMEASKLDARAKVV
                     LAEGDQIAITKIAEAVQANELPVMFILGGRYIDAMRNLSESSNAKFVVLPADIASALK
                     GMLGGSQGS"
     gene            complement(2210..>2411)
                     /locus_tag="PLT69_10010"
     CDS             complement(2210..>2411)
                     /locus_tag="PLT69_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ14814.1"
                     /translation="ALVGETGIVASLRPGESRGTLRFPLPIMGSDEWPFVSEAELSPG
                     DRVVVVDVSGNTLVVKKATRPG"
BASE COUNT          394 a          797 c          809 g          411 t
ORIGIN      
        1 cgatggcggc gtcatcctgc tggcctgcct ggggaaagac catgcgcagc gcgatcacga
       61 agagcatgat ccccccggcg atgccgagag acggctgggt gaggcccagg tacccgagga
      121 tcctcacccc ggcgacgagg aacacgacga ggacgaacag ggcgataaag agctccctga
      181 ggatgacccg ccgcctcgcc cgtacgggca cgttcgcgag cacggcgtga aaggtcggga
      241 tgttccccag ggggtcgagg atgaagaaga gggtcgccgc cgtggtgacg atgtcccttg
      301 cagagccttc acccatcctt gcctcccttg aggtctttgg tcatgcacgt tgcggactgc
      361 gggcagaggg aggtgaactg gccgcttgac cgtatctcgg cggggactgc gtccctggcg
      421 gtgatcgtgt aaccgagccg ctccatgaat ggagaggcgg tggtggtgag caggtacagg
      481 cgcgtaaccc ccagggagtc ggcgaggcgc tccgcctcgt ggaccacccc ggcgccgtga
      541 ccgcaccccc tgaacccggg gtgcacggcc accgaccgga ggaggcccga cgttccatgg
      601 ggttcgaccc ccgcgcaggc gaccagggtg ccggtgagag accggccctg gacgaaaccg
      661 cccattgtcc cggcagtcac gtcccgggcg tcgagcccgg ccgcctccag cagggatttc
      721 acctcggcga ggtcctcctt cgtgcacggc ccgaacgaga cggggcaggt ccttccgaag
      781 agcgacctca ggccgatcct tgtgggatgg cggccggaga gggacatctg gatgcaagcg
      841 agcctctcct ccacagggcc tatgggccag cagagggacg cgtccagcgc ggtcaggtcc
      901 acgaggggtt cgttgacggg gcagatgtcc cgcatctcca cgagagagcc gtctggaccc
      961 tgctccatcc acctcagggg caacccctgg gtgcggcaca cgtacggcct gtccgggtag
     1021 atgcggcagg cgccctcagg gtcgagaaag gcgcatgcgc cctgggggtg ggctgatccc
     1081 gcctcgagga gggccttgtt gttcgtcgcg atcgtcgaag cctccacctc gaagaccgtg
     1141 atcccgtcca cgcagcacga gctgcacccc cgcctgcacc tgagacggcc ggccaggaga
     1201 gaggccgtcc tcgaggcgag gcggtcgatc tcccggtaga gggcctgcag gctctcgagg
     1261 gcctccttcc gccttgccgt ctgtcccgag gcctccgcct ggcggaggga agcctgaggg
     1321 aagggccccc ggctcatgag ccttgcgatc ccccgagcat gcccttgagg gccgatgcga
     1381 tatccgcagg cagcaccacg aacttcgcgt tcgacgactc cgacaggttc ctcatggcgt
     1441 cgatgtacct gcctccgagg atgaacatca cggggagctc gttcgcctgc acggcctcgg
     1501 cgatcttggt gatggcgatc tggtcgccct cggcgagcac gaccttggcg cgcgcgtcga
     1561 gcttgctcgc ctccatgcgg ccctcggcct ggaggatggc cgcagccttc tccccctcgg
     1621 ccctggtcac cgtcgccctg cgggccctct cggccgcagc ctgctcctcc atcgccttct
     1681 gcatggtgtc ggaagggctg atgtcctgga tctccacggt cttgagcatg atcccccagt
     1741 ccgcgatgtc gtccgagatg ctctccttga gccgggcctt gatcttctcc cgcgacgaca
     1801 gggcgtcgtc caggttcatc tcgccgatga tggacctgag cgatgtctgc accaggttct
     1861 ggatggcgac gcggtagtcc tccacgccgt agaccgcctt ctcgggagag atgatgttga
     1921 tgtaggccac ggcgttcacc acgatcaccg cgttgtcctt ggtgatgacc tcctgggagg
     1981 ggatgtccag gacgatgtcc ttggtggtga ccttgtacgc gaccgtgtcg atgtagggga
     2041 tgatgaggtt caggccgggt ccgagggttg tgtggtactt tccgagcctc tgcaccacgt
     2101 gcttgctgcc ctggggcacg atcctcacgc ccagaaagag tgtgacgaga atgagcagga
     2161 cgaagaccgc aaccacgaac agtcccgaca tacgggcctc cttgttctgt tatcctggcc
     2221 gggtggcctt cttgaccacg agggtgttgc ccgagacgtc cacgaccacg accctgtccc
     2281 cgggagagag ctcggcctcc gagacgaagg gccactcgtc ggatcccatg atgggcagag
     2341 ggaagcggag ggtccccctc gactcgccgg gacggagact cgccacgatg cccgtctcgc
     2401 ccaccagcgc c
//
LOCUS       DAOSCR010000436         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28758_length_2406_cov_3.625266, whole genome shotgun sequence.
ACCESSION   DAOSCR010000436 DAOSCR010000000
VERSION     DAOSCR010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28758_length_2406_cov_3.625266"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLT69_10015"
     CDS             <1..499
                     /locus_tag="PLT69_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14815.1"
                     /translation="VFAPDGFFKTGDIGRFDEEGFLIITDRKKDLFVTSGGKNVAPHP
                     IELELQSRRFIEQACLVEDGRKYICALIVPDFEEIRRYAKERGVAYTDDADLVSKPEI
                     RSLVKAEVDAVNERLSRYEQVKYFELLSAPFSEETGELTPTLKVKRKVVAEKYKDLIE
                     GMYKH"
     gene            complement(474..1064)
                     /locus_tag="PLT69_10020"
     CDS             complement(474..1064)
                     /locus_tag="PLT69_10020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ14816.1"
                     /translation="MEHEWNRIGSRIAFRNKLVNVRLDTYHFLPNDIVKDFTVFELGD
                     WVNVIPMTSQGLVVTIRQYRHGVMRETVEIPGGLISKEDAGPMEAALREMTEETGYTS
                     REVIHIGTVEPNPAIQTNRCYTFLALDCLPDATQNLDPTESVRLELVHPDEVFRMIKD
                     GTITHALVVAAFCHLLIHEAASRGVPSPLNAYTCPR"
     gene            1386..1610
                     /locus_tag="PLT69_10025"
     CDS             1386..1610
                     /locus_tag="PLT69_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14817.1"
                     /translation="MKCVYMDEQCYEFHEDDSGERCFLCRQNSQRLFVVRQVESMKMV
                     HLCGECMVSNSDAFLLDNTRPWEGLKGKSE"
     gene            complement(1614..>2406)
                     /locus_tag="PLT69_10030"
     CDS             complement(1614..>2406)
                     /locus_tag="PLT69_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOJ14818.1"
                     /translation="PKPFTPDQIRAVTKRGLEHRSLKKEAERLREEKERMERSFITFV
                     SHEMRSPLVTVMQYFETLKAIAGSELSDASRDIIERCEKRLQALHDLIEHWLDLSRIQ
                     DGSFSKAQAPLVVTDVVEKSIEELSPLCQRREISLEYTPRQDAPRILGDAESLTRVFS
                     NIIGNSTKHTPQGGKISVGIDFDEFYVYVRISDTGEGIPEDKLPFIFEPFFRVKGKEE
                     RQRGSGLGLTFVKKIMDAHMGKIEVTSKLGEGTTFVLTFPRLYEL"
BASE COUNT          451 a          699 c          775 g          481 t
ORIGIN      
        1 ggtgttcgcg ccggacggct tcttcaagac cggggacata gggaggttcg acgaagaggg
       61 attcctcatc atcacggaca ggaagaagga cctcttcgtg acatccggag gcaagaacgt
      121 ggccccgcat cccatcgagc tcgagctcca gtcgaggcgc ttcatcgagc aggcctgcct
      181 cgtggaagac gggaggaagt acatctgcgc gctcatcgtg cctgatttcg aggagattcg
      241 aagatacgcc aaggaacggg gggtcgccta cacggacgac gccgatctcg tgtccaagcc
      301 ggagatccgc tccctggtga aggccgaggt ggacgccgtg aacgagaggc tgtccaggta
      361 cgagcaggtc aagtacttcg agctcctgag cgccccgttc agcgaggaga ccggcgagct
      421 cacgcctacg ctcaaggtga agcgcaaggt ggtggcggag aaatacaagg acctcatcga
      481 gggcatgtat aagcattgag aggggagggg accccccggc tcgcggcctc gtgaatgagc
      541 aggtggcaga acgcggccac cacgagcgca tgggtgatcg ttccgtcctt gatcatacgg
      601 aacacctcgt cggggtgcac gagttccagg cggacggatt ccgtggggtc gaggttctgg
      661 gtcgcatcgg gcaggcagtc cagggcgagg aaggtgtaac accggttggt ctgtatcgcc
      721 gggttcggtt ccaccgtgcc tatgtggatc acctcccggc tcgtgtagcc cgtctcctcg
      781 gtcatttccc tgagcgccgc ctccatgggt ccggcgtcct ccttggagat cagaccgccc
      841 gggatctcca cggtctcgcg catgacgccg tgtcgatact ggcggatggt gaccacgagc
      901 ccctggctcg tcatggggat gacgttcacc cagtccccca gctcgaagac cgtgaagtct
      961 ttcacgatgt cgttgggcag gaagtgatag gtgtccaggc gaacgttgac gagcttgttg
     1021 cgaaaggcga tcctcgagcc gatacggttc cactcgtgtt ccatcgtgac agcatccttt
     1081 ctcgcgtgtc gttttctcgg gcaatcctcc gcccatggag ctataccacg aaagaggggc
     1141 tccttgcacc gcgaaccgcc aacgatcgtg ccggcgcaag cgcccccgcc gagccgaagg
     1201 ggtgggcagg gacaggcctc aaatgggcct tcgagtgcag aggccgcgaa atgccgtcct
     1261 ttgtccgtga cctgcgattc acctcccgtc cttcgacgac tacctggcag ggtgttgcgg
     1321 ccttgtgcgg gccgtcgact tgtgttacag gaaaggatta cgtcgtctgg tcaggaaggg
     1381 gcgtcatgaa gtgcgtgtac atggacgagc agtgttacga gttccatgag gacgactcgg
     1441 gcgagcggtg ttttctgtgc aggcagaact cgcagaggct cttcgtcgtc cgccaggtgg
     1501 aaagcatgaa gatggttcac ctgtgcgggg aatgcatggt gagcaacagc gacgcctttc
     1561 tccttgacaa cacgaggccg tgggaggggc tgaagggaaa gagcgagtga ggcctagagc
     1621 tcgtagagcc tcgggaacgt gagcacgaac gtcgtgcctt ctcccagttt cgacgtcacc
     1681 tcgatcttgc ccatgtgagc gtccatgatc ttcttcacga aggtgagccc caagcccgag
     1741 ccgcgttgac gttcctcctt gcccttgacc ctgaagaacg gctcgaagat gaaggggagc
     1801 ttgtcttccg ggataccctc gcccgtgtcc gagatcctca cgtacacgta gaactcgtcg
     1861 aaatcaatgc cgacggagat cttcccgccc tggggggtat gcttcgtcga gttccctatg
     1921 atgttcgaga acaccctggt gaggctttcc gcgtcaccca ggatcctggg cgcatcctgc
     1981 ctcggcgtgt actcgagaga gatctcgcgg cgctggcaca ggggggacag ctcctcgatg
     2041 ctcttttcca ccacgtccgt gaccacaagc ggcgcctggg ccttggagaa ggacccgtcc
     2101 tggatccgag agaggtcgag ccagtgttcg atgaggtcgt gcaacgcctg aaggcgcttc
     2161 tcgcaccgtt cgatgatatc cctggaggca tcgctgagct ctgagcccgc tatggccttg
     2221 agcgtctcga agtactgcat gacggtcacg aggggggagc gcatctcgtg gctcacgaag
     2281 gtgatgaagc tgcgctccat ccgttctttt tcctccctca accgctcggc ttccttcttg
     2341 agggaacggt gttcgaggcc ccgcttggtc accgccctga tctggtcggg cgtgaagggc
     2401 ttcgga
//
LOCUS       DAOSCR010000437         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28800_length_2404_cov_2.885057, whole genome shotgun sequence.
ACCESSION   DAOSCR010000437 DAOSCR010000000
VERSION     DAOSCR010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28800_length_2404_cov_2.885057"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1078
                     /locus_tag="PLT69_10035"
     CDS             161..1078
                     /locus_tag="PLT69_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ14819.1"
                     /translation="MDETAMRGIPRSLNDEEGPCVPEGLPPVVDAHVHVFPDKLFDTV
                     RAWFDAFAWDIRYRMPSRDVVSYLLERGVEHVVALQYAHKAGISRRLNEYMAELCSEF
                     HPRVTGLATVFPGEDGCKDILREAFAMGLKGVKLHAHVQCFDVDGRPSREIYEVCASE
                     NKPLVVHASREPKSNAYLCDPYQLCSATKVERVLRDFPGLRLCVPHLGVDEFEAYRRL
                     LERFDNIWVDTAMVMAGYFPVEPPFTLCELRRDRVMYGSDFPNIPYAWDRELSRIRLS
                     GLPGDFLERLLHANAGEFFGISTGRAPRS"
     gene            complement(1050..1832)
                     /gene="fliR"
                     /locus_tag="PLT69_10040"
     CDS             complement(1050..1832)
                     /gene="fliR"
                     /locus_tag="PLT69_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOJ14820.1"
                     /translation="MSDLLRLAWGDFLTLFMVIVRVGMVFAVVPFFSAEVIPRRVTAV
                     LALFLSMVLMPVVPRVPVGIDELDALTLLVMVIQQLLVGIALGLAVNVIFAGVQIAGE
                     LMGFQMGFSIANVVDPMTGVTAPITSNLLYVTAFLLFFALGGHHQLIKGLFESFAIVP
                     ASGVLAREGFFTSTLAYASEMFVLGVKAASPVVGVLLLVNVAFAVLARAVPQMNVFLM
                     AFPITIAVGLVFLAVVVMMLPGLMEASLQKAQAFMNAVLALY"
     gene            complement(1829..2098)
                     /gene="fliQ"
                     /locus_tag="PLT69_10045"
     CDS             complement(1829..2098)
                     /gene="fliQ"
                     /locus_tag="PLT69_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOJ14821.1"
                     /translation="MTPETVTTMMAEAIKITLIVAAPMLLLGLVVGLCISVFSAVTQI
                     QEMTLTFVPKIVAVFLALLAALPWIIEKLVTYTTNLFASIPTLVR"
     gene            complement(2095..>2404)
                     /locus_tag="PLT69_10050"
     CDS             complement(2095..>2404)
                     /locus_tag="PLT69_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HOJ14822.1"
                     /translation="KADLELFIGLSKTARPKNRDDVPTRALIPAFMISELRTAFQIGF
                     ILFIPFLIIDMVVSSILLSMGMMMLPPVMISLPFKVMLFVLVDGWNLLVGSIVRGFAG
                     "
BASE COUNT          509 a          732 c          791 g          372 t
ORIGIN      
        1 ctcaagggtt cacccgcacg acgggaaggg atacccggca aagcaggtgc acggtgtgcc
       61 cgcgggtaag gggcgcaatc cgaatccgaa acggatgcac gcccgagcgg ggcttgctct
      121 cgaggcccgg gctggaagga gaggagggaa ggactgcaag atggacgaga cggcgatgag
      181 aggcataccg agatccttga acgatgaaga gggcccgtgc gtccccgaag ggctcccccc
      241 tgtcgtcgac gcgcacgtcc acgtctttcc ggacaagctc ttcgataccg tcagggcatg
      301 gttcgacgcc ttcgcgtggg acatcaggta caggatgccc tcgcgcgacg tggtgagcta
      361 cctccttgaa aggggcgtcg agcacgtggt cgccctccag tacgctcaca aggccggcat
      421 ctcccggcgc ctgaacgagt acatggcgga gctctgcagc gagtttcacc cccgggtgac
      481 cggcctcgcc acggtgttcc cgggtgagga cggctgcaag gacatcctga gggaggcctt
      541 cgccatgggt ctcaaggggg tgaagctgca cgcccacgtg cagtgcttcg acgtcgacgg
      601 caggccctcc cgcgagatct acgaggtatg cgcctcggaa aacaagcccc tcgtcgtgca
      661 cgcaagcagg gaaccgaaga gcaacgccta cctgtgcgac ccctaccagc tctgcagcgc
      721 gacaaaggtc gagcgggtcc tcagggactt cccgggactg aggctgtgcg taccccacct
      781 cggcgtggac gagttcgagg cgtacaggcg cctgctcgag aggttcgaca acatctgggt
      841 cgacacggcc atggtcatgg caggctattt ccccgtcgag ccgccgttca cgctctgcga
      901 gctcaggcgc gaccgcgtca tgtacggctc ggacttcccc aacataccgt acgcatggga
      961 cagggagctc tcgcggatca ggctctcggg cctgcctgga gacttcctcg aacggcttct
     1021 ccatgcgaac gccggggagt tcttcgggat cagtacaggg cgagcaccgc gttcatgaat
     1081 gcctgcgcct tctggaggga cgcctccatg aggccgggca gcatcatcac gacgaccgcg
     1141 aggaagacga gacccacggc gatggttatg ggaaaggcca tgaggaacac gttcatctgg
     1201 ggcaccgccc gcgcaagtac cgcgaacgcg acgtttacga gcaggagcac gccgaccacg
     1261 ggggaagccg ccttgacacc gagcacgaac atctccgagg cataggcgag cgtcgaggtg
     1321 aagaaccctt cccttgccaa gacgccgctt gcggggacta tcgcgaacga ctcgaagagc
     1381 cccttgatga gctggtggtg gcccccgagc gcgaagaaca ggaggaatgc ggtcacgtac
     1441 aggaggttcg aggttatggg cgccgtgacc cctgtcatgg ggtcgaccac gttcgcgatg
     1501 gaaaagccca tctggaaccc catgagctcg cccgcgatct ggacgcccgc aaagatcacg
     1561 ttgactgcga gcccgagggc gataccgacg aggagctgct ggatcaccat cacgagcagg
     1621 gtgagcgcgt cgagttcgtc gatgcccacc gggaccctcg gcacgacggg catgagcacc
     1681 atgctgagaa agagcgcgag caccgcggtg acacggcggg gtatgacctc ggcgctgaag
     1741 aacggcacca cggcgaagac catccccacc ctcacgatca ccatgaagag ggtcagaaag
     1801 tcgccccagg cgagtctcaa caggtcgctc atctcacgag ggtcggtatg ctggcgaaca
     1861 ggttggtggt gtaggtcacc agcttctcga tgatccaggg cagggccgcg agcagggcga
     1921 gaaagaccgc gacgatcttg ggcacgaagg tgagcgtcat ctcctggatc tgggtcacgg
     1981 ccgagaacac gcttatgcag agcccgacca ccaggcccaa aaggagcatg ggcgcggcca
     2041 cgatgagggt gatcttgatg gcctcggcca tcatggtcgt gacagtctcc ggcgtcatcc
     2101 cgcaaacccc ctgacgatag atccgacgag caggttccac ccgtcgacga gcacgaagag
     2161 catgaccttg aagggaagcg atatcatgac cggcggcagc atcatcatgc ccatggacag
     2221 caagatcgac gagaccacca tgtcgatgat gagaaacggt atgaagagga tgaacccgat
     2281 ctggaaggcc gtcctgagct cgctgatcat gaacgcgggg atgagcgccc tcgtgggcac
     2341 gtcgtccctg ttcttgggcc tggcggtctt cgacaggccg atgaagagct cgaggtcggc
     2401 cttg
//
LOCUS       DAOSCR010000438         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28890_length_2399_cov_3.550768, whole genome shotgun sequence.
ACCESSION   DAOSCR010000438 DAOSCR010000000
VERSION     DAOSCR010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28890_length_2399_cov_3.550768"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLT69_10055"
     CDS             complement(<1..199)
                     /locus_tag="PLT69_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ14823.1"
                     /translation="MTKSDLVNAIAQKTGKSKVASAEAVEAILGAIKAALKKGDKVTL
                     VGFGTFEVAKRAARDGVNPRTG"
     gene            complement(383..1231)
                     /gene="panC"
                     /locus_tag="PLT69_10060"
     CDS             complement(383..1231)
                     /gene="panC"
                     /locus_tag="PLT69_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOJ14824.1"
                     /translation="MDVITTVRGMKEYARAIKDRRETICLVPTMGYLHEGHLELMRKG
                     RTLARHLFASIFVNPTQFGPSEDLARYPRDFERDRRLCEDVGVECIFYPSVEEMYPAS
                     YSTYVDVEGVTRHLCGASRPGHFRGVATVVAKLFNICMPDVAVFGEKDYQQLVVIRRM
                     VRDLDMDVQVVAHPTVRESDGLAMSSRNTYLTPQQRMEALVLKKAIDRAKELVGQGER
                     DTSRIKKAALDLIGEAPSASVDYVEIVDPDTVEPVGTIDDRAVIALAVRIGSTRLIDN
                     AELKAP"
     gene            complement(1247..2050)
                     /gene="panB"
                     /locus_tag="PLT69_10065"
     CDS             complement(1247..2050)
                     /gene="panB"
                     /locus_tag="PLT69_10065"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOJ14825.1"
                     /translation="MRKPVTVLDILAKKGKEPVVMVTSYDATMARIVDEADVDIILVG
                     DSAGMVMAGLENTLGVSMDMMVYHTLCVTRVKPKALVVADMPFLSYQAGVHDAVVNAG
                     RLIQEGCAQAVKVEGGMRVIDQVRAIVKADIPVMGHLGLTPQSVHAFGGYKVQGRSEA
                     AAQRLKEDALALEDAGVFSIVLECVPTSLAEEVTRSLVVPTIGIGAGPACDGQVLVIQ
                     DLLGMSKEFKPKFVKRYAELFDSIKDAVTTYAREVRSRAFPSEEHCFKG"
BASE COUNT          403 a          803 c          710 g          483 t
ORIGIN      
        1 tgccggtcct cgggttgacc ccgtccctgg ccgcacgctt tgcgacctcg aacgtcccga
       61 agccgaccag ggtgaccttg tcgcccttct tcagcgcggc cttgatcgcg ccgagaatgg
      121 cctcgaccgc ctcggcggac gccaccttgg acttgcccgt cttctgagcg attgcattca
      181 ccagatccga ctttgtcata cgctatcccc ctctgaaacg tttattttct tgcttgagcg
      241 accatgagat cgcgacaagc aaggtgcaaa cacaatcttc ggcaattcaa ctataaatca
      301 ttaagtattt tttgtaactg caacagcagg tcgatgcaaa cgctcatctg gcctacatcc
      361 aagcggatga tgcatggtcg gatcatggcg ccttcaactc cgcattgtcg atgagcctcg
      421 tggacccgat ccttacggca agcgcgatga cagccctgtc gtcgatggtc cccacgggct
      481 cgacggtgtc cgggtcgacg atctccacgt agtccacgct tgcggaaggc gcctctccta
      541 tgaggtcgag ggcggccttc tttatgcgag aggtgtcgcg ctccccctgc cccacgagtt
      601 ccttcgccct gtctatcgcc ttcttgagca ccagggcttc catgcgctgc tggggcgtca
      661 ggtaggtgtt cctcgagctc atcgcgagcc cgtcgctctc cctgaccgtc gggtgggcca
      721 cgacctgcac gtccatgtcg aggtccctca ccatacggcg gatgaccacg agctgctggt
      781 agtccttctc tccgaacacg gccacatccg gcatgcagat gttgaagagc ttggccacca
      841 cggtggccac acccctgaag tgcccgggcc tcgaagcccc gcacaggtgc ctggtgacgc
      901 cctcgacatc cacgtaggtg gagtacgacg cggggtacat ctcctcgacg ctcgggtaga
      961 agatgcattc cacgccgacg tcctcgcaga ggcgcctgtc gcgctcgaag tcgcggggat
     1021 acctggcgag gtcctcgctg gggccgaact gtgtcgggtt gacgaagatg ctcgcgaaga
     1081 ggtgccgggc gagagttcgc cccttgcgca tgagttcgag gtgtccttcg tggaggtatc
     1141 ccatggtggg cacgaggcag atggtttcgc gcctgtcctt tatggccctg gcgtattcct
     1201 tcatgcctcg caccgtggtg atcacgtcca tccggccccc tccaccctag cccttgaagc
     1261 agtgctcctc ggaagggaac gccctgctcc tcacctcgcg ggcgtacgtg gtcactgcgt
     1321 ccttgatgga gtcgaagagc tcggcatacc tcttgacgaa cttcggcttg aactccttgc
     1381 tcatgccgag aagatcctgg atgaccagga cctgcccgtc gcatgcaggt ccggctccaa
     1441 tgccgatggt gggcacgacc agcgacctgg tcacctcctc ggcgagggac gtgggcacgc
     1501 actcgaggac gatggagaag acaccggcgt cttccagggc gagggcgtcc tccttgagcc
     1561 tctgggcggc cgcttcggac ctcccctgga ccttgtaccc gccgaaggcg tgcaccgact
     1621 gcggggtgag cccaaggtgg cccatcacgg ggatatcggc cttgacgatg gccctcacct
     1681 ggtcgatgac gcgcatgccg ccctcgacct tcacggcctg ggcacaccct tcctggatca
     1741 gcctcccggc gttgaccacc gcgtcgtgga cgcctgcctg gtaggaaagg aacggcatgt
     1801 cggcgacgac gagggccttc ggcttcaccc tcgtgacgca gagggtgtgg tagaccatca
     1861 tgtccatgga gacgcccagg gtgttttcga ggccggccat gaccatgccg gcggaatcgc
     1921 ccacgaggat gatgtcgacg tcagcctcgt caacgatccg cgccatggtc gcgtcgtagg
     1981 aggtgaccat cacgacgggc tctttgccct tcttggcgag gatgtcgagg accgtgactg
     2041 gctttctcat tccccctctt ccctgaacat agattacacg tcaatcttca ggcgatcaag
     2101 tctttttctc ccgctgacca cggatcttca agatgggggt gcccctttcc ccacctccca
     2161 agcggtttcc gagtgccgag cccgcctcga aggcgggtct gaaaggggct cgagcacgcg
     2221 gccgcgtcgt ggcggaatcg gggagaccgg ctccacgcct cgatcgcgag gccacgccca
     2281 agaaacccgt gccggccccc ctgtcgtgca tcgagcggct cccggccggt tccgccgacg
     2341 gaatggatga ccctctcccg cccccccgtg catatgcgcc cgctcatggg gcagccacc
//
LOCUS       DAOSCR010000439         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28922_length_2397_cov_3.091375, whole genome shotgun sequence.
ACCESSION   DAOSCR010000439 DAOSCR010000000
VERSION     DAOSCR010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28922_length_2397_cov_3.091375"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLT69_10070"
     CDS             <1..747
                     /locus_tag="PLT69_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ14826.1"
                     /translation="ESGSISASEFDAVSARYEAASAALDAGRAEVLARQAALENARVA
                     VAYTEIRAPFAGVVLTKNADVGDIVTPIGAAANAKAAVVTIADLDSLQVEADVSESNI
                     TLVGTGKPCDIELDALPGERFAGVVDAVVPTVDRAKATVLVKVRFTGIDRRILPGMSA
                     KVSFLPRDVRAEDRRARPMVDARSVKATGGRHVVFVVEGGRAHLREVTLGRRLKDWVE
                     IPSGPAPGSLVVVDPPEGLRDGSRVKIGAP"
     gene            970..1665
                     /locus_tag="PLT69_10075"
     CDS             970..1665
                     /locus_tag="PLT69_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ14827.1"
                     /translation="MTGAQDHQPLVRVEHVSKSYRRGDIVVRVLRDIHLEIAQGEFLA
                     IMGPSGSGKSTLLNLIAGIDRVDEGTITVGGRVVTSMNETELARWRSRNVGFVFQFYN
                     LIPVLTAFENVELPLSLTALPRKERIEHVEAALSLVGLSDRMDHYPAQLSGGEQQRVA
                     IARAIVTDPVIIVADEPTGDLDRASAEEVLDLMTRLNEDMGKTIVMVTHDPHASGRAH
                     VVRHLDKGEFDSR"
     gene            1662..>2397
                     /locus_tag="PLT69_10080"
     CDS             1662..>2397
                     /locus_tag="PLT69_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ14828.1"
                     /translation="MILLKMIVRNALRRPLRTALTVAAVAVAILAFTLLRALLAAWYS
                     AVDASSASRLVARNAVSIMFPLPISYADRMRHVEGVARVSYGSWFGGIYVSEKNFFAN
                     FAVDAQSYLDLYPELVLPAKEKADFLRDRRGFVAGRKLVERFGWHIGDIVTLKGTLYP
                     GEWDFVLRGVYEGKTRTVDETQFFFHWQYLNERLKDSFPSMADTVGFFMIGVKDPQSA
                     AAVARSIDSAFKNSRAETLTESERAFV"
BASE COUNT          412 a          751 c          857 g          377 t
ORIGIN      
        1 gagtccggct cgatatccgc ctcggagttc gacgcggtga gtgcgaggta cgaagccgcc
       61 tcggccgccc tggatgcggg aagggcggag gtcctggccc gtcaggcggc gctcgagaac
      121 gcgagggtcg cagtcgcata caccgagatc agggcgccct ttgcgggtgt ggtcctcacg
      181 aagaacgcgg atgtcggcga catcgtcacc cccatcggcg cggcggccaa cgcgaaggcg
      241 gccgtggtga cgatcgccga tctggactcg ctccaggtgg aggcagacgt gtccgagtcg
      301 aacatcaccc tcgtggggac gggcaagccc tgcgacatcg agctcgacgc gctgccgggc
      361 gagagattcg cgggggtggt ggacgccgtc gtgccgacgg tggacagggc gaaggccacg
      421 gtgctcgtga aggtgcggtt caccggcatc gaccgtcgca tccttcccgg catgagcgcg
      481 aaggtctcgt tcctcccgag ggatgtcagg gcagaggaca gaagggccag gcccatggtc
      541 gacgcccgct cggtcaaggc gacgggcggg aggcacgtgg tcttcgtggt ggagggcggc
      601 agggcgcacc ttcgcgaagt caccctgggc aggcgtctga aggactgggt ggagataccg
      661 tccggtccgg ccccgggcag cctcgtggtg gtggatcctc ccgagggcct gagagacggc
      721 tcgcgggtga agatcggtgc gccgtgaggc aggggctcga gacaccggtc caagcgcccg
      781 ggcgttcggg tgccgcgggc gaggacgggg tattccggtg ggcgtgaccc aagggcccga
      841 gcggccacgg ggcgcctcga gcgaggctgc tccccccgtg cgggggatca cgccgggggg
      901 ctgaagggcc ggtgacacgg ccagaaatgc cgcgtgtcga aatgatcagt accgggagga
      961 agatccggcg tgacaggggc ccaggaccac cagccgctcg taagagtcga gcacgtgtcc
     1021 aagtcgtata ggcggggaga catcgtcgtc cgggtcctca gggacataca cctcgagata
     1081 gcgcaaggcg agttcctcgc tatcatgggg ccctcggggt cgggaaagag cacgctcctc
     1141 aacctgatcg cgggcatcga ccgcgtggac gaggggacca tcaccgtggg cggccgcgtg
     1201 gtcacgtcca tgaacgagac cgagctcgcg cggtggcgct ccagaaacgt cggtttcgtg
     1261 ttccagttct acaacctcat cccggtgctc acggccttcg agaacgtgga gctcccgttg
     1321 agcctgaccg ccctcccgag gaaggaacgc atcgagcacg tcgaggccgc gctgtccctg
     1381 gtggggcttt cggacaggat ggaccactac ccggcgcagc tctccggcgg ggaacagcag
     1441 cgggtggcca tagcgagggc catcgtgacc gaccccgtga tcatcgtcgc cgacgaaccc
     1501 acgggcgacc tcgacagggc ttccgccgag gaggtgctcg acctcatgac gcggttgaac
     1561 gaggacatgg gcaagaccat cgtgatggtg acgcacgacc cccacgcatc cggccgtgcg
     1621 cacgtggtga ggcacctgga caagggcgag ttcgacagcc gatgatcctg ctcaagatga
     1681 tcgtgaggaa cgcgctgagg cgccccttgc ggaccgccct caccgtcgcg gccgtggcgg
     1741 tcgcgatcct cgccttcacc ttgctgagag ccctgctcgc tgcctggtat tcggccgtgg
     1801 acgcctcttc cgcctcccgg ttggtcgcgc ggaacgcggt ctccatcatg ttcccgctgc
     1861 cgatatccta cgcggacagg atgaggcacg tcgaaggggt ggcgcgcgtc tcgtacggga
     1921 gctggttcgg gggcatctac gtgagcgaga agaacttctt cgcgaatttc gccgtggatg
     1981 cccagagcta cctggacctc taccccgagc tcgtcctgcc ggccaaggag aaggcggact
     2041 tcctcaggga ccgccgcggg ttcgtcgccg ggaggaagct cgtcgagcgc ttcgggtggc
     2101 atatcggcga catcgtcacc ctgaagggga cgctctaccc tggagagtgg gatttcgtgc
     2161 tgaggggcgt ctacgagggg aagacccgta ccgtcgacga gacccagttc ttcttccact
     2221 ggcaatacct gaacgagagg ctcaaggact cgttcccctc catggccgac accgtgggct
     2281 tcttcatgat cggggtgaag gacccccagt ccgccgcagc ggtcgcccgc tccatagaca
     2341 gcgccttcaa aaactcgagg gctgagacgc tcaccgagag cgagcgcgcg ttcgtct
//
LOCUS       DAOSCR010000440         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_28923_length_2397_cov_2.973954, whole genome shotgun sequence.
ACCESSION   DAOSCR010000440 DAOSCR010000000
VERSION     DAOSCR010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28923_length_2397_cov_2.973954"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..2330
                     /gene="glgX"
                     /locus_tag="PLT69_10085"
     CDS             258..2330
                     /gene="glgX"
                     /locus_tag="PLT69_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOJ14829.1"
                     /translation="MEMNVLPGKPYPLGATVYPHGVNFSLFSKNSYEVELLLFDDPDD
                     ARPSRVIRLDPKTNRTFYYWHCFVTGLEPGQLYGYRVYGPFDPARGHFFDGQKVLVDP
                     YALSVVLGKNYEREAACRPGDNCGHAPKCAVVDPKAYDWEGDEPLRHDYRTSVIYELH
                     VGGFTRHRNANVSPGKRGTYAGLVEKIPYLKELGITAVELMPVQQFDEQDAPPGLSNY
                     WGYSPMAFFAPHGGYSSKGGAAGPMDEFRDLVKALHRSGIEVIIDVVYNHTAESGIDG
                     PTLSFRGLENTYYYIYDIEHRRYENFSGCGNTVNTNHSIVRRMITESLKHWVKHMHVD
                     GFRFDLASVMARGERGEPLERPPILWSIESDPVLAGTKIIAEAWDAAGLYQVGSFIGE
                     RFAEWNGQFRDDVRRFVKGDTGEVYRLMNRLMGSPDIYPQPERETNRSINFVTCHDGF
                     TMNDLVSYNTKHNEANLEDNKDGNDFNFSWNCGVEGPTDDPFVEELRLRQIKNFFTIL
                     FISQGTPMILMGDEVRRTQHGNNNAYCQDNEISWFDWDLVAKNRELLGFVKGLIRFSR
                     SHSVFNMEHIPRQPSRDRSVDIRWHGVRLNKPDMSEYSHSIACELVSFQEPEHVFFMI
                     NAFWEPLAFELPKPLSGHAWHRVVDTSLAAGQDYCPPGKAPRLTSRRYLVKDRSVAVL
                     VSRRVKPG"
BASE COUNT          510 a          720 c          757 g          410 t
ORIGIN      
        1 gggcacgccg ttgttgtcca tgtgccggga ggatccttca gaaagggagt gcatcatgaa
       61 gggagtgcat cgtgggcctt gaatcctttg gtactttgca acaagaggtc ttgactcgcg
      121 gggtaagccg tggaagaagg tgtctcattg cgacttgaag aaaggtcggt cgtgtgtgag
      181 gcgaaccccc atccttgccg ccaagggggc ttgcctccca tgaggcggca ggagacagag
      241 acgaagaggc gccctcgatg gagatgaacg tgcttcccgg caagccgtac cccctcggtg
      301 ctacggtata ccctcatggt gtcaatttct ccctgttctc caagaacagc tacgaggtgg
      361 aactgctcct cttcgacgac ccggacgacg cgaggccctc tcgagtcatc aggctcgacc
      421 ccaagacgaa caggaccttc tactactggc actgcttcgt caccggcctc gagccggggc
      481 agctctatgg ataccgggtg tatgggccct tcgaccccgc ccgggggcac ttcttcgacg
      541 ggcagaaggt gctcgtggac ccgtatgcgt tatccgtggt gctggggaag aactacgaga
      601 gggaggctgc ctgcaggccg ggggacaact gcgggcacgc accgaagtgc gcggtggtcg
      661 accccaaggc gtacgactgg gagggcgacg agcccctgag gcacgactat cgaacgagcg
      721 tgatctacga gctccacgtg ggcggcttca cccggcaccg aaacgcgaac gtgtcgcctg
      781 gaaagagggg cacctacgca gggctcgtcg agaagatacc gtacctgaag gagctcggga
      841 tcacggcggt ggagctcatg ccggtgcagc agttcgacga gcaggatgcg ccgcccggcc
      901 tctccaacta ctggggatac agccccatgg ccttcttcgc gccgcatggg gggtacagct
      961 cgaagggtgg cgccgccggg cccatggacg agttcaggga cctggtgaag gccctgcaca
     1021 ggtctggcat agaggtgatc atcgacgtgg tgtacaacca caccgccgag tcgggcatcg
     1081 acggcccgac gctgtcgttc aggggcctcg agaacaccta ctactacatc tacgacatag
     1141 aacacaggag gtacgaaaac ttcagcgggt gcgggaacac ggtgaacacg aaccactcca
     1201 tcgtccgcag gatgatcacg gagtcattga aacactgggt caaacacatg cacgtggacg
     1261 gtttcaggtt cgacctcgca tccgtcatgg cgcgcggtga gcggggcgaa cccctggaac
     1321 ggccgcccat cctctggtcc atcgagtctg accctgttct ggccggcacg aagatcatcg
     1381 ccgaggcatg ggacgctgca gggctctacc aggttgggtc gttcatcgga gagcgtttcg
     1441 cagagtggaa cgggcaattc cgcgatgacg tccgaaggtt cgtcaagggg gacacgggcg
     1501 aggtgtaccg gctcatgaac cgcctcatgg gaagcccgga catctacccc cagcccgagc
     1561 gcgagacgaa caggagcatc aacttcgtca cctgccacga cgggttcacc atgaacgacc
     1621 tcgtgtcgta caacaccaaa cacaacgagg cgaacctcga ggacaacaag gacggcaacg
     1681 atttcaattt cagctggaac tgcggcgtcg aaggcccgac ggacgacccg ttcgtggaag
     1741 agttgcgctt gaggcagatc aagaacttct tcaccatcct gttcatctcc cagggcacgc
     1801 cgatgatcct catgggcgac gaggtgagga ggacccagca cgggaacaac aacgcgtact
     1861 gccaggacaa cgagatctcg tggttcgact gggatctcgt cgccaagaac agggaactgc
     1921 tcgggttcgt caaggggctc atcaggtttt ccaggtcgca ctcggtcttc aacatggagc
     1981 acatcccacg gcagccgagc agggacaggt cggtcgatat caggtggcac ggcgtgcgcc
     2041 tgaacaaacc ggacatgtcg gagtactccc acagcatagc gtgcgagctc gtctccttcc
     2101 aggagcccga gcacgtcttc ttcatgatca atgcattctg ggaacccctc gcgttcgagc
     2161 tccccaagcc cctgtccggc cacgcatggc acagggtcgt ggacacgtcc ctcgcagccg
     2221 gccaggacta ttgccccccg ggaaaggccc ccaggctcac ttctcgaagg tatctcgtca
     2281 aggaccgaag cgttgcggtg ctcgtgtcca ggagggtgaa acccggttga tcccccctgg
     2341 gcggcgttca tgggagccgc accagggaaa gacacgcaag cgagctcggc ggacggc
//
LOCUS       DAOSCR010000441         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29013_length_2391_cov_4.364298, whole genome shotgun sequence.
ACCESSION   DAOSCR010000441 DAOSCR010000000
VERSION     DAOSCR010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29013_length_2391_cov_4.364298"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLT69_10090"
     CDS             <1..311
                     /locus_tag="PLT69_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14830.1"
                     /translation="RGRVSTYYCANPACRVTFFDASLGYACPECGAFGIISRYVSDTC
                     TQDPAGRQVVGCIDSIGRLFCSACATQYDLFQDASMLVFSDSLPHARQSCDACRGRLT
                     "
     gene            958..1689
                     /locus_tag="PLT69_10095"
     CDS             958..1689
                     /locus_tag="PLT69_10095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ14831.1"
                     /translation="MDTIRINRFLSMCGAVSRRKADDLVRSGRVKINGARARTGDTVD
                     PERDIVTLDGRRVLPQPQVILAFYKPKNVLTTLSDPQGRPCLGDLIPEHLRGVFPVGR
                     LDYDASGLLILTNDGDLAHAIHHPSFMVPKTYVVKVRPRAAEEAVRAMEAGVVIDGKK
                     TMPARVRVVHSTPRESILNITLTQGLKNQIKRMASCVGLRVVEIKRTSVGPVTLRGMA
                     PGEMRPLSAREARALHNILKKGYKP"
     gene            1751..2035
                     /locus_tag="PLT69_10100"
     CDS             1751..2035
                     /locus_tag="PLT69_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ14832.1"
                     /translation="MNKSELIEELSMRMALHPKKAEALVNSILNSMINAMKEKRRVEI
                     RGFGSFVMRDYKPYIGRNPKSGEKIQVKAKTLPFFKAGKDMRDRVNGKGI"
     gene            2128..>2391
                     /locus_tag="PLT69_10105"
     CDS             2128..>2391
                     /locus_tag="PLT69_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ14833.1"
                     /translation="MANHTILIVDDEAPNREYLCELLSEEGYTVSSAANGREAVARLS
                     QEHFHVVLTDLQMPELDGLGVIQHIVERKINTIGILYTGYASVK"
BASE COUNT          491 a          773 c          756 g          371 t
ORIGIN      
        1 acaggggcag ggtcagcacg tactactgcg ccaacccggc ctgcagggtc acgttcttcg
       61 atgcgtccct ggggtatgcc tgcccggagt gcggcgcctt cggcatcatc agccggtacg
      121 tgagcgacac gtgcacccag gacccggccg gcaggcaggt cgtgggctgc atcgactcca
      181 tcggccgtct cttctgttcg gcatgcgcca cacagtacga cctgttccag gacgcgagca
      241 tgctcgtctt ctcggacagc ctgccccacg cgcggcaatc ctgcgacgca tgcaggggac
      301 gcctgacctg agggcgccac gtggggcggc gccctgacca cgaagaagtg gcggatgggc
      361 gatctccact cgtgaggcgg ccatactatc gaggccttca ggcacacctc gctcgggtgg
      421 gatggatgac cgcttaggcc gtgtgcacgc ctgcccggcc caccttccac cccgggcctg
      481 accgggggag atacctgcct tcatggattc caccgggacc tgcctgaccg ggggacacgg
      541 cccccgatcg acgctgtggg taggatgtga cccccgacgg aaccggcacc gctttccatc
      601 ccctggtgca cgaacgggca ggcgttcgcc cctccaggag cgcgtctacg ccccggccag
      661 acaacgagcc gggacccctt ccgggatacc ggtcccgtgg atgctgtgtt ccttaccggt
      721 tgattctgca acagcagcaa gagggggtac cgggcccgga gaggctgcat acctgccggg
      781 cccggtgcgc tagcgccgcc ggggcaacca tttggggcct gaataggggt aattcccaaa
      841 ccccagggcc caagcgcagc cggggcaccc tggggtccct cgaggaaggg gtgcataacc
      901 cgggcgaggc ccgtcacact ggcaagggca tcgctgttgc gctataaggc atgacccatg
      961 gacacgatac gcatcaacag gttcctctcc atgtgcgggg ccgtctcgag aaggaaggcg
     1021 gacgacctcg tgaggtccgg gagggtgaag atcaacggcg cgagagcccg gacgggcgac
     1081 accgtggatc ccgagaggga catcgtcacc ctcgacggca ggagggtcct gccccagcca
     1141 caggtgatcc tcgcgttcta caagcccaag aacgtcctca ccacgctcag cgacccccag
     1201 gggaggccat gcctgggaga cctcataccc gagcacctcc ggggcgtctt ccccgtgggc
     1261 aggctcgatt acgacgcttc aggcctcctc atcctcacca acgacggaga cctcgcccat
     1321 gccattcacc acccgtcgtt catggtgccg aagacctacg tggtgaaggt gaggccgcgc
     1381 gccgccgagg aggcggtccg ggcgatggag gcgggcgtgg tcatagacgg gaagaagacg
     1441 atgccggcgc gagtgagggt ggtccactcg acccccaggg aatcgatctt gaacatcacc
     1501 ctcacccagg ggctcaagaa ccagatcaag cgcatggcat cgtgcgtcgg cctgcgggtc
     1561 gtggagatca agcgcactag cgtcgggccc gtgaccctga ggggcatggc gccgggcgag
     1621 atgcgcccct tgagcgcgcg agaggccagg gccctgcata acatcctaaa aaaaggatat
     1681 aaaccttgac acagggtgcg agttccttat aatcatacag gaacaccagt tcaccgcgag
     1741 gaggagacgc atgaacaagt cggaactgat tgaggaactc tccatgcgca tggccctcca
     1801 ccccaagaag gccgaggccc tcgtcaactc gatcctcaac tccatgatca acgccatgaa
     1861 ggagaagcgt cgcgtggaga tacgggggtt cggcagcttc gtcatgagag actacaagcc
     1921 atacatcggc cgcaacccca agagcgggga aaagatacag gtcaaggcca agaccctccc
     1981 cttcttcaag gccggcaagg acatgcggga cagggtgaac ggcaagggca tctgagcgag
     2041 accgcccctt tcgcaaccgt ttcccatcga agagcgtcta tatattgacg cacctttcac
     2101 gttacgctaa agtactgtaa caggaccatg gcgaaccata ccattctcat cgtcgacgac
     2161 gaggcgccca acagggagta cctctgcgag ctgttgagcg aggaggggta cacggtctcg
     2221 agtgcggcca acggcaggga ggccgtcgcg cggctgtccc aggaacactt ccacgtggtc
     2281 ctgaccgacc tccagatgcc ggagctcgac gggctcggcg tgatccagca catcgtggag
     2341 cggaagatca acaccatcgg catcctctac acgggatacg cctcggtcaa g
//
LOCUS       DAOSCR010000442         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29127_length_2385_cov_2.911588, whole genome shotgun sequence.
ACCESSION   DAOSCR010000442 DAOSCR010000000
VERSION     DAOSCR010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29127_length_2385_cov_2.911588"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /gene="dnaE"
                     /locus_tag="PLT69_10110"
     CDS             <1..1546
                     /gene="dnaE"
                     /locus_tag="PLT69_10110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ14834.1"
                     /translation="PMELIPLYADRRHKRQPVEYPHPALEDILKETYGIIVYQEQVMQ
                     IAQQMAGYTLGRADLLRRAMGKKDRVEMATQRQAFISGATARGVDEAKAGEIFDLMEK
                     FADYGFNKSHAAAYALVAYQTAYLKAHHFKEFMAANLTLDVNNRDKVARHLAECRNKG
                     VQVLGPDINASSFEFVVGPEGIRFGLGAIKNVGRTAVEAILEERDRSGEFSDIAEFMD
                     RVPLLKVNRRVVEALVKAGAFDRLHPNRRSVFEALDELFDRAQRRARTTNDAQITLFS
                     LDEFSPQDARTPVRLPDIPDWPEPERLRMELDGMGYYLSGHPLDKYAHLARKYATATT
                     AGLEEASSPVTLAGVLTEVKVSKTKKGDTMARARLEDLEGSVQVIFFPQVFARHQDLI
                     LKPDDPVLVRAALKVEEDQENGEAEQASIELIAEELWKLEHAEEALARRVVVKVRRPL
                     SRREIEELKASVLSCKGGCALTFEVETEEALVAIDAGKDYSVMPGREFIERVTGILGR
                     GGVELQ"
     gene            1543..>2385
                     /locus_tag="PLT69_10115"
     CDS             1543..>2385
                     /locus_tag="PLT69_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ14835.1"
                     /translation="MKTKDRFVDIDRALKDLQESISRIRGIDTSEIERKVDSFRRELN
                     EGISRWSCVELSRHAMRPGVDDFIAGIFTRFTELKGDRFMEDDPAVVGGPAFLDDTPV
                     MVVGHRKRTPGKKDYVHYHYGMSGPSGHYKALRLYRLAEKFSRPIVTFVDTPGAYPAP
                     EAEERGQAFSIALCIQALSSVRTPVVACIIGEAGSGGALALGYGDRIIMLENAFYSAI
                     SPEGFSSIVFKDAKRKELAAETLRGTGRDLYAAGIVDYLVKEPVGGAHNAPGKVVEEV
                     GKALR"
BASE COUNT          463 a          739 c          826 g          357 t
ORIGIN      
        1 ccccatggaa ctcatcccgt tgtatgccga ccgcaggcac aagcggcagc cggtcgagta
       61 cccccacccc gcgctggagg acatcctcaa ggagacctac ggcatcatcg tctaccagga
      121 acaggtcatg cagatagccc agcagatggc ggggtacaca ctgggaaggg cggatctcct
      181 gaggcgggcc atggggaaga aggacagggt ggagatggcg acccagcgcc aggccttcat
      241 ctccggcgcg acggcccggg gggtggacga ggccaaggcg ggcgagatct tcgacctcat
      301 ggagaagttc gccgattacg ggttcaacaa gtcgcacgcg gccgcctacg cgctcgtcgc
      361 ctaccagacg gcctacctga aggcccacca cttcaaggag ttcatggccg cgaacctgac
      421 gctcgacgtg aacaacaggg acaaggtcgc caggcacctt gccgagtgcc gcaacaaggg
      481 ggtccaggtc ctcggccccg acatcaacgc cagcagcttc gagttcgtgg tgggacccga
      541 gggcatccgc ttcggactgg gggcgatcaa gaacgtcggc cgcacagcgg tcgaggcgat
      601 cctcgaggaa cgggacaggt ccggggagtt ctcggacatc gccgagttca tggacagggt
      661 ccccctactc aaggtcaaca ggcgcgtggt ggaggccctc gtgaaggccg gtgcattcga
      721 caggcttcac ccgaacaggc gctccgtgtt cgaggccttg gacgagctct tcgacagggc
      781 gcaaaggcgg gcgagaacca cgaacgacgc ccagatcacc ctcttcagcc tcgacgaatt
      841 ctccccccaa gacgcgagga cccccgtccg tctgcccgac atccccgact ggcccgagcc
      901 ggagcgcctc aggatggagc tcgatggcat ggggtactac ctgtcgggac atcccctgga
      961 caagtacgcc cacctcgcac gcaagtacgc gacggccacc accgcgggcc tcgaggaggc
     1021 gtcgtcgccg gtgacgctcg ccggggtgct caccgaggtg aaggtatcga agaccaagaa
     1081 gggcgacacg atggcgaggg cgaggctcga ggacctggag gggtcggtgc aggtgatctt
     1141 cttcccccag gtcttcgcca ggcaccagga tctcatcctc aagcccgacg acccggtgct
     1201 cgtgagggcc gcgctcaagg tggaggagga tcaggagaac ggtgaggcgg agcaggcgag
     1261 catcgagctc atcgccgaag aactctggaa gctcgagcat gccgaggaag ccctcgcgag
     1321 gcgtgtcgtg gtgaaggttc gccggccgct ctcccgcagg gagatcgagg aactcaaggc
     1381 ctcggtcctg agctgcaagg gcgggtgcgc gctcaccttc gaggtggaga cggaggaggc
     1441 cttggtggcc atcgacgcgg ggaaggatta ttcggtcatg ccgggcaggg agttcatcga
     1501 gcgcgtgacc gggatcctgg gtcgcggggg ggtggagctg cagtgaagac gaaggaccgt
     1561 ttcgtcgaca tcgaccgtgc cctcaaggac ctgcaggaga gcatatcccg catcaggggc
     1621 atagacacct ccgagataga gcgcaaggtg gattccttcc gccgggagct caacgagggc
     1681 atctcgcgct ggtcgtgcgt ggagctgtcg aggcacgcca tgagaccggg cgtggacgat
     1741 ttcatcgccg ggatcttcac ccgcttcacc gagctgaagg gggaccgttt catggaggac
     1801 gacccggcgg tggtaggcgg cccggccttt ctcgacgaca cgccggtcat ggtggtgggc
     1861 caccggaagc gaacgccggg caagaaggat tacgtccact accattacgg catgtcgggc
     1921 ccttccggcc actacaaggc gctcaggctc tacaggctcg ccgagaagtt ctcgaggccg
     1981 atcgtgacct tcgtggacac gccgggcgcc tacccggccc ccgaggccga ggaacgcggc
     2041 caggccttct ccatagccct gtgcatacag gccctctcgt ccgtgaggac accggtggtg
     2101 gcctgcatca taggggaggc gggttccggc ggggcgctcg cgctcggcta cggcgacagg
     2161 atcatcatgc tcgagaacgc cttctacagt gccataagcc ccgagggctt ctcgtccatc
     2221 gtcttcaagg acgccaagag gaaggagctc gcggcggaga ccctcagggg gacggggagg
     2281 gatttgtatg ccgccggtat cgtggactac ctcgtcaagg agccggtggg cggggcccac
     2341 aacgcccccg ggaaggtcgt cgaggaggtg ggcaaggctc tgcgc
//
LOCUS       DAOSCR010000443         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29159_length_2383_cov_2.839347, whole genome shotgun sequence.
ACCESSION   DAOSCR010000443 DAOSCR010000000
VERSION     DAOSCR010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29159_length_2383_cov_2.839347"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..2211)
                     /locus_tag="PLT69_10120"
     CDS             complement(28..2211)
                     /locus_tag="PLT69_10120"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14836.1"
                     /translation="MNKKTREMIVVGAMFVAMILMWVLKTNLDTIGHVPTSNVVIFTI
                     ISVNVILLVLVVFLVTRNVVKLMLERRRGVLGTRLRTKLVVAFVTLTIIPTTVLFFAS
                     VVFIGRSVEGWLSAEVEKAIAESMNVANVYYREASADAIRYASSISGEITERRLLKEE
                     NLPFLRSALKEKMDLFRFSSVVVFSAQGEELVRMTSPGLENVRMPTLESATVQKAMDG
                     RSLSMVVRKGKADFIEGIVPIRSSFNPRDVVGVLVADYYIPESLSERLNLIKRSFSDY
                     SQILRLKGPIKTNYIIILTSVTLLVIFSAIWFGINTAKGLINPIEKLVEGTQRISRGD
                     LGFTIDVQAEDELGILVNDFNAMVEDLKESRRNLESAYNEIQARNRYTMSVLNAISTG
                     VITITRDDRIALVNPSAQALLGLRQAEVAGKPASEVLSGEYSQLYTELVQSLRTSRGH
                     GVVSRQINVQAGATRLTLMCKASFLFAENSESMGILIVFDDLTQLMRMQRMAAWREVA
                     RRIAHEVKNPLTPIQLSAQRLRRRYLDKLGDDAEVIEECTRVIINEVDVMKRLVNEFS
                     AFAKMPSAMLRPDDLNRVVSETVALYRQAHAEVEYLLDLDENLPPIEIDHAQMNRAVG
                     NLLDNATTAVIENNGDNPKKIEVRTRFDPELKIAVLDIADNGPGIPPGARDRIFEPYF
                     STTKGGTGLGLAIVNRIVADHNGYIRVRDNEPSGTVFSIELPVKE"
     gene            complement(2208..>2383)
                     /locus_tag="PLT69_10125"
     CDS             complement(2208..>2383)
                     /locus_tag="PLT69_10125"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOJ14837.1"
                     /translation="GLRFEDEFVRHKILDLLGDLWSLQAPLKARITAWKASHALHVRL
                     AKALFEEHRGERA"
BASE COUNT          390 a          737 c          762 g          494 t
ORIGIN      
        1 cgactatgag aatggtcctc ttcatgctca ctccttgacc ggcagttcta tgctgaagac
       61 cgtgcccgaa ggctcgttgt ccctgaccct gatgtacccg ttgtgatccg ccacgatgcg
      121 gttcacgatg gcgagcccca accccgtgcc gcccttggtc gtggagaagt acggctcgaa
      181 gatccggtcc ctggcaccgg gcgggatgcc gggcccgttg tccgcgatgt ccaggacggc
      241 gatcttgagt tcggggtcga agcgtgtcct cacctcgatc ttcttggggt tgtccccgtt
      301 gttctcgatc acggcggtgg tcgcgttgtc caggaggttt cccacggcgc ggttcatctg
      361 tgcgtggtcg atctcgatgg ggggcaggtt ctcgtccagg tcgagcaggt actccacctc
      421 ggcgtgcgcc tgcctgtaga gggccaccgt ctccgacacc acccggttca ggtcgtccgg
      481 ccggagcatg gcgctcggca tcttcgcgaa cgccgagaac tcgttgacca ggcgcttcat
      541 gacgtccacc tcgttgatga tcacgcgggt gcactcctcg atgacctccg cgtcgtcgcc
      601 cagcttgtcg aggtacctcc tcctgaggcg ctgggcggac agctggatgg gggtgagcgg
      661 gttcttgacc tcgtgggcga tcctccgggc cacctcgcgc catgccgcca tgcgctgcat
      721 gcgcatgagc tgggtgaggt cgtcgaacac gatgaggatc cccatgctct cgctgttctc
      781 cgcgaacagg aaggaggcct tgcacatgag ggtgagcctc gtggcccccg cctgcacgtt
      841 gatctgcctg gacacgaccc cgtggcccct cgacgtcctc aacgactgca cgagctcggt
      901 gtagagctgg gagtattccc ccgagaggac ctcggaggcg ggctttcccg ccacctcggc
      961 ctgcctgaga ccgaggaggg cctgggccga ggggttgacg agggcgatgc ggtcgtccct
     1021 cgtgatggtg atgacacccg tggagatggc gttcagcacg ctcatggtgt aacggttcct
     1081 cgcctggatc tcgttgtagg cgctctcgag gttccgcctg gattccttga ggtcctccac
     1141 catggcgttg aagtcgttca ccaggatccc gagctcgtcc tctgcctgga cgtctatggt
     1201 gaagcccagg tcgcccctgg agatccgctg ggtcccctcg acgagcttct cgatggggtt
     1261 gatgaggcct ttcgcggtgt tgatgccgaa ccagatggcg gaaaagatga cgagcagcgt
     1321 caccgaggtg aggatgatga tgtagttggt cttgatgggt cccttgagcc tgagtatctg
     1381 ggagtagtcc gagaaggacc tcttgatgag gttgaggcgc tccgagaggc tctccgggat
     1441 gtagtagtca gccacgagca cgcccacgac gtccctgggg ttgaaggacg acctgatggg
     1501 cacgatgccc tcgatgaagt ccgccttgcc cttcctgacc accatggaga ggctcctgcc
     1561 gtccatggcc ttctgtaccg tcgcggattc caaggtcggc atcctgacgt tctcgaggcc
     1621 gggggaggtc atcctcacga gctcttcccc ctgggcggag aacaccacca ccgaggagaa
     1681 cctgaacagg tccatctttt ccttgagggc cgacctgagg aaggggaggt tctcctcctt
     1741 gaggagcctc cgctcggtga tctccccgga tatggacgag gcgtagcgga tggcgtccgc
     1801 cgacgcctcc cggtagtaca cgtttgccac gttcatggac tccgctatgg ccttctccac
     1861 ctccgcagac agccagccct ccacgctcct gccgatgaac accacgctcg cgaagaacag
     1921 gaccgtggtc gggatgatgg tgagggtgac gaaggccacc acgagcttcg tcctgagccg
     1981 cgtcccgaga accccgcgcc tgcgctcgag catgagcttg accacgttgc gggtgaccag
     2041 gaagaccacg agcacgagca ggatcacgtt gacgctgatg atggtgaaga tgacgacgtt
     2101 gctcgtgggt acgtggccta tggtgtcgag gttcgtcttc aggacccaca tgaggatcat
     2161 ggccacgaac atggccccga ccacgatcat ctccctggtc ttcttgttca tgccctttcc
     2221 ccccggtgct cttcgaagag ggcctttgcg agccgcacgt ggagtgcgtg actcgccttc
     2281 caggcggtga tgcgggcctt gaggggcgcc tggagtgacc agagatcgcc caggagatcg
     2341 aggatcttgt gcctgacgaa ctcgtcctcg aacctgaggc ctt
//
LOCUS       DAOSCR010000444         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29219_length_2379_cov_2.868330, whole genome shotgun sequence.
ACCESSION   DAOSCR010000444 DAOSCR010000000
VERSION     DAOSCR010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29219_length_2379_cov_2.868330"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /gene="meaB"
                     /locus_tag="PLT69_10130"
     CDS             <1..846
                     /gene="meaB"
                     /locus_tag="PLT69_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOJ14838.1"
                     /translation="RACVVGVTGTPGAGKSTLVGHVGRSLLERGHALGIVAVDPSSPY
                     TGGALLGDRLRMRDISSHEGVFIRSMATRGALGGLCPAARDVCRLMEANGRDVVIIET
                     VGVGQDEIEVVSASDWVVLVTFPGQGDAIQAIKAGVMEIADIFVVNKADREGAGEMAS
                     AIEAMLDLATGPGQERPPVFMTSCLSGEGVSALSDHVEDLVSRRKDEPARDRRRVAVE
                     LMDLLDTHVRGILRERLGRDGIYERAVDAVMEGRADPYEAAWSLAESLGFVGGPLDAD
                     WGGRP"
     gene            843..2183
                     /locus_tag="PLT69_10135"
     CDS             843..2183
                     /locus_tag="PLT69_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOJ14839.1"
                     /translation="MTGGQKGFTRRLAAFVAQTDSGSIPKDAYDHAKVAFLDWLGVTL
                     AGKDDPLVGKLVELARLVGGQPQATLVGLGMKTDVAHAALVNGSASHALDYDDTLVSF
                     LGHPSVTLFPALLALSEWKGSGGRDFLASYIIGLQAGGTVGACASLDHYLAGWHATST
                     LGHIASAAACAKLLGLDEERTCWAIGIGATQSCGLKRVFGTMCKPFHAGRASQAGLTA
                     ALLAGMGFTCADDTIEGPQGFFDALKGKVNEEIVGMLGLGWDVVNLSQKYHASCHATH
                     SPMEAALEAVGTNSIDIDDIESITVHSSELALQAAAKQDPETGLEGKFSISYCVANAL
                     LRGETGMQAFSDEKVRDGHVRQLMKRIQVIHDPQIKALESIVEVRTKDGKVKTAFSDI
                     LQQIPPLETKRTRVRSKFLDLAVPAVGRDRAETILERSEHLEDESMTTFAQGLA"
     gene            2253..>2379
                     /locus_tag="PLT69_10140"
     CDS             2253..>2379
                     /locus_tag="PLT69_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOJ14840.1"
                     /translation="MAGRVLVTDIARFAVNDGPGFRTNVFLKGCPLACAWCHNPET"
BASE COUNT          437 a          719 c          858 g          365 t
ORIGIN      
        1 agggcctgcg tggtgggggt cacggggaca cccggggcgg gcaagagcac gctggtcggg
       61 cacgtcggca ggtcgctgct cgagaggggc catgccctgg ggatcgtggc ggtcgacccg
      121 tccagcccct acacgggagg cgcgctgctc ggcgaccggc tcaggatgcg agacatcagc
      181 tcgcacgagg gtgtgttcat caggtccatg gcgaccagag gcgcgctcgg gggactctgc
      241 cccgcggcca gggacgtgtg caggctgatg gaggccaacg gcagagacgt cgtcatcatc
      301 gagacggtcg gtgtcgggca ggacgagatc gaggtcgtga gcgcgtcgga ctgggtggtg
      361 ctcgtcacct tccccgggca gggggacgcg atacaggcga tcaaggccgg cgtcatggag
      421 atcgcggata tcttcgtggt gaacaaggcg gatcgagaag gagccggtga gatggcctcc
      481 gccatcgagg cgatgctcga cctcgcgaca ggcccggggc aggagagacc ccccgtgttc
      541 atgacgtcgt gcctcagcgg ggaaggggtc tcggccctct ccgaccacgt ggaggacctg
      601 gtgtcgagac gcaaggacga gcctgcgaga gacaggagga gggtggccgt ggagctcatg
      661 gaccttctcg acacccacgt cagggggatc ctgcgggaaa gactcggccg cgacggcatc
      721 tatgagaggg cagtggacgc ggtcatggaa gggagggcgg acccgtacga ggccgcgtgg
      781 tcccttgccg agagcctggg gttcgtgggc gggcccttgg atgcggattg gggggggaga
      841 ccatgaccgg tggacagaag ggattcacca ggcgtctcgc ggcgttcgtc gcgcagacgg
      901 acagcgggtc catcccgaag gatgcctacg atcacgcgaa ggtcgcgttc ttggactggc
      961 tgggggtcac gctcgccggg aaggacgacc ccctcgtcgg caagctcgtc gagctcgcac
     1021 gactcgtcgg cggtcaaccc caggccacac tcgtcggcct tggaatgaaa acggacgtgg
     1081 cccacgccgc cctcgtcaac ggcagcgcgt cccacgccct ggactacgac gacaccctcg
     1141 tctctttcct cggccaccca tccgtgaccc tcttccctgc gctgctcgcc ctttccgaat
     1201 ggaaggggtc gggcgggagg gatttcctgg cctcctacat catcggtctc caggcggggg
     1261 ggacggtggg cgcgtgcgcg agcctcgacc actacctcgc cgggtggcac gccacctcca
     1321 cgctcgggca catcgcctca gcggcggcct gcgcgaaact cctcgggctc gacgaggaga
     1381 ggacgtgctg ggcgatcggg atcggcgcga cccagtcgtg cggactcaag cgcgtcttcg
     1441 ggaccatgtg caagcctttc catgccggga gggcctccca ggcgggtctc accgcggcac
     1501 tcctggctgg gatggggttc acgtgcgcgg atgacaccat cgaaggcccg cagggattct
     1561 tcgatgcgct caagggaaag gtcaacgagg agatcgtcgg catgctgggg ctcgggtggg
     1621 acgtggtcaa cctgtcccag aagtaccacg cttcctgtca cgccacccac tccccgatgg
     1681 aggccgcgct cgaagcggtc gggacgaatt ccatcgacat cgacgacatc gagtccataa
     1741 ccgtgcactc ctcggaactc gccctgcagg ctgcggccaa gcaggacccg gaaaccggcc
     1801 tggagggaaa gttcagcatc tcctattgcg tggcgaacgc cctcctgaga ggcgagacgg
     1861 gaatgcaggc gttttcggac gagaaggtgc gagacgggca cgtgagacaa ctcatgaaac
     1921 gcatccaggt gatccatgac ccacagatca aggcgctgga gtccattgtc gaggtccgca
     1981 cgaaggacgg caaggtgaag accgccttct cggacatcct ccagcagata ccgcctctcg
     2041 agacgaagcg cacaagggtg aggagcaagt tcttggactt ggccgttccc gcggtgggta
     2101 gggacagggc cgagacgatc ctcgagcggt cggaacacct ggaagacgag agcatgacta
     2161 cgttcgcgca ggggctggca tgaggcggtc gccttccaga cgacagggaa gaggtcgagg
     2221 gggagatgcc ccgggaaccg ggagatcttg ccatggcagg tagggtgctc gtgacggaca
     2281 tcgcgaggtt cgcggtcaac gacgggccgg ggttcaggac gaacgtgttc ctgaagggtt
     2341 gcccccttgc gtgcgcatgg tgccacaacc ccgagacca
//
LOCUS       DAOSCR010000445         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29258_length_2376_cov_3.321413, whole genome shotgun sequence.
ACCESSION   DAOSCR010000445 DAOSCR010000000
VERSION     DAOSCR010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29258_length_2376_cov_3.321413"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLT69_10145"
     CDS             <1..417
                     /locus_tag="PLT69_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_144016486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOJ14841.1"
                     /translation="EKDLRIDTYRSSGHGGQHVNKTDSAVRITHLPTGIVVQCQNERS
                     QHKNKAFAMKLLASKLYELEETKQKERLSEFTKGKKEIAWGSQIRSYVLQPYQMVKDH
                     RTNLEVGNVDAVLDGDIAQFIDAFLMKQATGGGARA"
     gene            410..910
                     /locus_tag="PLT69_10150"
     CDS             410..910
                     /locus_tag="PLT69_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ14842.1"
                     /translation="MLDLIVSRRSIRSFEPRPVGEGLVERILEAGRWAPSGMNNQPWR
                     FAVITDRGLVEQVSRLTRYSRILAQAPVLVAVFLDMEASYHRTKDVQAIGACIQNMLL
                     EAHAEGLGAVWIGEILRSEAELKELLEAPEGFELMAVLAVGHPRGPAPRPPARKALEE
                     LVFFRK"
     gene            934..2088
                     /locus_tag="PLT69_10155"
     CDS             934..2088
                     /locus_tag="PLT69_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOJ14843.1"
                     /translation="MPVETIRLGDREITLVGTAHVSKSSVEEVRETILSVRPDVVAVE
                     LCSQRRDVMRNPGLWQETDIFEVIRKKKASFLLANLVLSSFQRRIGKRLGVKPGDEMR
                     AAMEEAEASGIEVALVDRDVRVTLARAWRTLSFLEKVKLLWAALVAVFEADELGEDDI
                     ERLKEQDALTVAVDAMAKTVPTVKKVLVDERDAYMAKKIADLEARRVVAVLGAGHLAG
                     VAARLGDTSYDIGPLEEIPEGGFRYWGWVLPAAFVALVLAGFVTGGAASGIRMIAWWA
                     GITMACTALATALTMAHPVTVVVATLVAPLTTLHPALASGWFAGMSEAYFKRPRVVDF
                     ERIHEDILTLKGWWQNPITRILLVFFMSNLGSSVGVFIAAPVLARLAVRM"
BASE COUNT          428 a          703 c          868 g          377 t
ORIGIN      
        1 gagaaggacc tgaggataga cacgtaccgt tcctcgggac acggcggaca gcacgtgaac
       61 aagaccgact cggccgtccg catcacgcac ctccccacgg gcatcgtcgt ccagtgccag
      121 aacgagcgat cccagcacaa gaacaaggcc ttcgccatga agctcttggc gagcaagctc
      181 tacgagctcg aggaaacgaa gcagaaggag cgcctgagcg agttcacgaa gggcaagaag
      241 gagatagcct gggggtcgca gatccggtcc tatgtgctcc agccctacca gatggtcaag
      301 gaccacagga cgaatctcga ggtgggcaac gtggatgcgg tgctcgacgg ggacatcgcg
      361 cagttcatcg acgcctttct gatgaagcag gcgacggggg gaggcgcccg tgcttgacct
      421 catagtctcc cggcgcagca tacggtcgtt cgaacccagg ccggtggggg aagggctcgt
      481 cgagaggatc ctggaggcgg gcaggtgggc gccctcgggc atgaacaacc agccctggag
      541 attcgcggtg atcaccgaca ggggcctggt ggaacaggtg agccgcctca cgaggtattc
      601 ccggatcctc gcccaggcgc ccgttttggt ggccgtgttc ctggacatgg aggcgtcgta
      661 ccatcgcacc aaggacgtgc aggccatagg cgcctgcatc cagaacatgc tgctcgaggc
      721 gcacgcggag gggctcgggg cggtgtggat cggcgagatc ctgaggtccg aggcagagct
      781 caaggaactc ctcgaggcgc ccgagggctt cgagctcatg gcggtgttgg ccgtcggcca
      841 ccccaggggg ccggcaccga ggcctcctgc gaggaaggcg ctcgaagagc tcgttttctt
      901 caggaagtga cgcctgaaga ccggaggaca ggcatgcccg tcgagaccat acgccttgga
      961 gaccgcgaaa taacgctcgt ggggacggcc catgtctcga agagctcggt cgaggaggtc
     1021 agggaaacga tcctttccgt gaggcccgat gtcgtggccg tggagctctg ctcgcagcgt
     1081 cgcgacgtca tgaggaaccc gggcctgtgg caggagaccg acatcttcga ggtcatccgg
     1141 aagaagaagg cgtcgttcct gctcgcgaac ctcgtgctgt cgtcgttcca gaggcgcatc
     1201 gggaagcggc tcggggtgaa gcccggcgac gagatgaggg cggccatgga agaggcggag
     1261 gcatccggca tcgaggtggc cctcgtggac agggacgtgc gcgtgacgct cgctagagcc
     1321 tggaggacct tgagcttcct cgaaaaggtg aagctgttgt gggcggcact ggtggcggtg
     1381 ttcgaggcgg acgagctcgg cgaggacgac atcgagcgcc tcaaggaaca ggacgcgctc
     1441 acggtggcgg tggacgccat ggcgaagacg gtgccgacgg tcaagaaggt gctcgtcgac
     1501 gagcgtgacg cgtacatggc gaagaagatc gcggacctcg aggccaggag ggtggtggcc
     1561 gtgttggggg cggggcatct ggccggggtc gcggccaggc tcggtgacac ctcgtacgat
     1621 atcggtccgc tcgaggagat ccccgagggc ggcttcaggt actgggggtg ggtgctgccc
     1681 gcggccttcg tcgcgctcgt gctcgcgggc ttcgtcacgg gcggggccgc aagcgggatc
     1741 cggatgatcg cctggtgggc ggggatcacc atggcatgca ccgctctcgc cacggccctg
     1801 accatggccc acccggtcac ggtggtcgtg gccaccctcg tcgcgccttt gaccacgctc
     1861 caccccgcgc tcgcctccgg ctggttcgcg ggcatgagcg aggcctactt caagaggccg
     1921 agggtcgtgg atttcgaacg catccacgag gacatcctca ccctgaaggg gtggtggcag
     1981 aaccccatca ccagaatcct gctcgtgttc ttcatgtcga acctgggcag cagcgtgggc
     2041 gtgttcatag ccgctcccgt tctcgcgagg ctcgcggtga ggatgtgagc gaaccgctgc
     2101 gcttgtgccc cggggaaaac cccgcatccg gtgatgaaag tgccgtgggg gccctcgcgg
     2161 gagggcgtga caggtctgcc cctttccccg gaagaagggg tttggggctc tccgggggac
     2221 gccgtcgttg ccggtgtgac gcctcgtcaa ccggccccgg tttggatggc gggcggagcg
     2281 ctcgagccgg gatcccacgc gagccaggaa ggggtctcgc agaaggccgg caaagccacg
     2341 agaccttccc gctcgatggc tcggtggagg tggccg
//
LOCUS       DAOSCR010000446         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29360_length_2370_cov_2.719222, whole genome shotgun sequence.
ACCESSION   DAOSCR010000446 DAOSCR010000000
VERSION     DAOSCR010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29360_length_2370_cov_2.719222"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLT69_10160"
     CDS             <1..284
                     /locus_tag="PLT69_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ14844.1"
                     /translation="GACVSCGACVPACNTGAHTLEGGVHVFDQNRCIGCGLCVPACPT
                     GAIRTRPNPSYKPPAKNWLALALKNLPASLVNLVVTSVARRLRRAGPGR"
     gene            926..>2370
                     /locus_tag="PLT69_10165"
     CDS             926..>2370
                     /locus_tag="PLT69_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOJ14845.1"
                     /translation="MISNREEACTGTPREGDGYDRVGVSEGEEGQSFHSGADRFSPPI
                     NPFTLAFPGRLESEYLEDFYLKSLNQIRLILMSGILFYGIFGFLDAKLLPDAKWTLWV
                     IRYLVVIPCILSVIAFSFSKRFKRYMQLCLATAITVSGLGIVTMILIAKPPVNYSYYA
                     GLILVLIFGYVFLRIRFVWAAAAGWTIVAFYEAASIWLTNTPMEILLNNNFFLLSANV
                     IGMFACYSAEYHSRRDFYLVKLLTVEQDKVRAANLELERRVEERTAQLVRSNEELVKE
                     IEAHRRSEEEKRGLEHQLRQAQKMEAIGTLAGGIAHDFNNILSPIIGYAEMILDDEDQ
                     ASPIRRKVEQILMAASRAKELVRHILTFSRQTEQELLPMELHPIVKESLTLLRASLPS
                     TIEIRQKIDTSCSPVLADATQIHQIVINLCTNAFHAMRDKGGVLGVEMTEVAIDETDR
                     TNLKIKPGRYVRLSVSDTGTGIPKNILDRVF"
BASE COUNT          449 a          818 c          719 g          384 t
ORIGIN      
        1 agggggcctg cgtctcctgc ggcgcgtgtg tccccgcctg caacaccggc gcccatacct
       61 tggaaggggg ggtgcacgtc ttcgaccaga acaggtgcat cgggtgcggc ctgtgcgtgc
      121 cggcatgtcc cacaggggcc atccggacga ggccgaaccc ttcctacaag ccgccggcga
      181 agaactggct cgccctcgcg ctcaagaacc tgcccgcctc cttggtgaac ctcgtggtca
      241 cgagcgtcgc aagaaggctc aggcgggccg gtccaggccg ctgagcaggc acgaaccatg
      301 gacgcccctc tcaacagggg gcttccggct gggacctccg gtggcaggac gggatgggaa
      361 ggtcgttgac cgaggccacg catcaggggg ttgaccggca cggcctccgg acccggttcc
      421 gagcagcaaa gacgctgacc cacccaccgc accagggtgt cgcccaggca tgccggccgg
      481 gccctgccct gcttcccccc gcacgcccac gagggttcgt cccatgattc ggcaggttct
      541 caggcgcgac cccggctccg tctgcatgcc cgcctgcagt cgtgcgcgtc gaccccgaag
      601 gccggcatca cgcccatgcc ggcgcctcgc ctccaggttg tcgggctaca ggcgcagggc
      661 caggtggggc cacacctccc ctgccccggg cggtcctgca catcaaggcg atcctccctc
      721 gttttcccac aaggcatcac ggccgggccc acgggacctg tgcacgagac gctgccgcct
      781 cgcccgcggc aaccctgcac cgcacagccg ccctgcaccg agacgggcgc gcttggctgc
      841 atcgtgacaa ggatccgagc ccgcagggta ggcaaccggc acggatgtta actcatcggt
      901 caatcgatcc gatgaagagg aaaggatgat ctcgaacagg gaagaggcat gcacgggcac
      961 tccccgtgag ggggatggat acgacagggt cggcgtgtcg gaaggcgagg aagggcagtc
     1021 cttccattcc ggggccgacc ggttcagccc ccccatcaac cccttcaccc tggccttccc
     1081 cggcaggctc gagtccgagt acctcgagga cttctacctc aagtccctga accagatccg
     1141 cctcatcctc atgtccggta tcctcttcta cggcatcttc gggtttctcg acgcgaagct
     1201 cctcccggat gccaagtgga ccctgtgggt catccggtac ctcgtggtca taccctgcat
     1261 cctctcggtg atcgcctttt ccttctcgaa gaggttcaag cgctacatgc agctctgcct
     1321 cgccaccgcc atcacggtct cggggctcgg catcgtcacc atgatcctca tagcgaaacc
     1381 gcccgtgaac tactcctact acgccgggct catcctggtg ctcatattcg gctacgtgtt
     1441 cttgcggatc aggttcgtgt gggccgccgc cgcgggctgg accatcgtcg ccttctacga
     1501 ggccgcatcc atctggctca cgaacacgcc catggagatc ctcctcaaca acaacttctt
     1561 cctcctgagc gccaacgtga tcgggatgtt cgcctgctac tcggcggagt accactccag
     1621 gagggacttc tacctcgtga aactcctcac cgtcgagcag gacaaggtca gggcggcgaa
     1681 cctcgaactc gagaggaggg tggaggagag gaccgcccag ctcgtacgct ccaacgagga
     1741 gctcgtgaag gagatcgagg cgcacaggag gtccgaggaa gagaagcggg gcctcgagca
     1801 ccagctcagg caggcgcaga agatggaggc catagggacc ctggccggag ggatagctca
     1861 cgacttcaac aacatcctct cgcccatcat cggctacgcg gagatgatcc tcgacgacga
     1921 ggaccaggcg agcccgatca ggcggaaggt cgagcagatc ctcatggcgg cctcccgggc
     1981 gaaggagctc gtcaggcaca tcctcacctt cagccgccag actgagcagg aactcctgcc
     2041 catggagctc caccccatcg tgaaggaatc cctcaccctg ctgagggcct cgctgccctc
     2101 taccatcgag atcaggcaga agatcgacac ctcgtgttcg ccggtgctcg cggatgcgac
     2161 ccagatacac cagatcgtca tcaacctgtg cacgaacgcc tttcacgcca tgcgggacaa
     2221 gggcggcgtg ctcggcgtgg agatgaccga ggtcgccatc gatgagaccg accgcacgaa
     2281 cctcaagatc aaacccggca ggtatgtgag gctcagcgtg agcgacaccg ggaccgggat
     2341 ccccaagaac atcctggaca gggtgttcga
//
LOCUS       DAOSCR010000447         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29458_length_2365_cov_2.273160, whole genome shotgun sequence.
ACCESSION   DAOSCR010000447 DAOSCR010000000
VERSION     DAOSCR010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29458_length_2365_cov_2.273160"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PLT69_10170"
     CDS             <1..1226
                     /locus_tag="PLT69_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14846.1"
                     /translation="AEYARSLNRNAATDAADIVVYNPAGLVDLSKGYHLNISNQTWVR
                     RPSHSFDDPLGGGRLRFEQKKTDWIVPNVHAAYCGPGWALFAAAYIPAGGAAVDYPDG
                     SYSTRLLGAGIIGPGGPYELIYNDIDDEYLEGTSLYLALSAGAACRVTEGVSLAAGIR
                     TVSIRNEIRGGLTLTGGLLGPMTQDVPLRVRVSEEGRGWGVVCGIQVRPSDAVNLALH
                     YESPTRIDVRADVKSGDNVSQEAGLFSDGAESPRDLPAMVGLGASFRVGDRTRVELDA
                     NYWFQKDADWGRSADGRGLADLAGDSYSVGASVCHALNRDLEVSCGFLYTWYDFEDMD
                     GFYRNTTGAVEVYYENDLMIGLGFAYRVTPSITFNLGTGVITYRDAAISTGAGTVSVE
                     NDLSGALALGVDCSF"
     gene            1830..>2365
                     /locus_tag="PLT69_10175"
     CDS             1830..>2365
                     /locus_tag="PLT69_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOJ14847.1"
                     /translation="MAKISQWGEVRPTNPLKIQDVTFRDAHQSIFATRVRTQDLLVIA
                     EKMDRVGFHSMEVWGGATFDTMHRFLGEDPWERIRVLKRHMGRTPFSMLLRGQNLVGY
                     RNYPDDVAEAFVERCCINGIDIFRIFDALNDFRNFETVVRVVKEHGKHFQGAICYSLT
                     ERRMGGEVYTLEYYLDKA"
BASE COUNT          444 a          727 c          797 g          397 t
ORIGIN      
        1 gcgccgagta cgcgcggagc ctcaaccgca acgcggcgac agacgccgcc gacatcgtgg
       61 tatacaaccc ggccgggctc gtggacctct cgaaaggcta tcacctcaac atctcgaacc
      121 agacgtgggt gaggaggccg agccactcct tcgatgatcc cctcggcggg ggccgcctca
      181 ggttcgagca gaaaaaaacc gactggatcg tcccgaacgt ccatgccgcg tactgcggcc
      241 ccggctgggc cttgttcgcc gcggcctaca tcccggcagg cggggcggcc gtcgattatc
      301 cggacggctc gtattccacg cggctcctgg gcgccggcat cataggcccg ggcgggccgt
      361 acgagctcat ctacaacgac atcgacgacg agtacctgga ggggacctcg ctctacctcg
      421 ccctgtccgc cggcgcagcg tgcagggtca cggaaggcgt gagcctcgcc gcgggcatca
      481 ggacggtctc catcaggaac gagatcaggg gggggctcac gctcaccggg ggtctcctgg
      541 gtcccatgac ccaggacgtg ccgctcaggg tgagggtgag cgaggagggc aggggctggg
      601 gcgtcgtgtg cggcatccag gtgaggccct cggacgcggt caacctcgcc ttgcattacg
      661 agtcgcccac gaggatcgac gtcagggcgg acgtgaagtc cggcgacaac gtgagccaag
      721 aggcagggct gttctcggac ggggcggaga gcccgaggga cctgcccgcg atggtgggcc
      781 tcggcgcatc cttccgtgtc ggcgacagga ccagggtgga actcgatgcc aactactggt
      841 tccagaagga cgcagactgg ggccgctcag ccgacggaag gggcctggcg gacctcgcgg
      901 gcgactccta cagcgtgggc gcctcggtat gccacgccct gaacagggac ctcgaagtca
      961 gctgcggctt cctgtacacg tggtacgact tcgaggacat ggatgggttc tacaggaaca
     1021 ccaccggggc cgtggaggtg tactacgaga acgatctcat gatcggcctc ggcttcgcat
     1081 accgggtcac cccctcgatc accttcaacc tcggcaccgg ggtcatcacg taccgggacg
     1141 cggccataag cacgggtgca ggcacggtgt ccgtggagaa cgacctctcg ggcgcgctgg
     1201 ccttgggagt ggactgttct ttttagaccg ggacgcccct ttagagctcg gaacgggcct
     1261 gcatcggccg ggtgggcatc gagagagcgg ctcggcctcg tccattgagg ccggttcgag
     1321 atgtccgggt tgtcggggct gatcggtcac ccatgcagtc ctgcatgctt tcctttttct
     1381 cggtgacgcg tgcgttccgg ccgtggcgca agggcacacg tcatccatgc tggcctgcat
     1441 gcttggcctt ttcgtggggt cccccacctg cagatggcat ccttcccggc ccaagaacga
     1501 tggcggtggt tgcccgaaag tgtcccacct tgtcgcctgt cggttccatc ctgcaggatc
     1561 gcctgtaaga acaatatgtt gcgcccgatg agaagcgctt cggggtcatg gccgcaccag
     1621 ggggatgggc cggggcggca gcaaccctct aaaaatcaaa aagaataaca atatgataca
     1681 gggtgcgatg ccgtggcacc catattgctg tccaggctgt cagggggtgg gcgcggccac
     1741 gtgggcacgg cgcggccgca agccctcccg atcacacggt gtgattgaac aggggggtcg
     1801 cggtgaagac gacgagacat ggcaccctgg tggcaaagat cagccagtgg ggggaggtac
     1861 gccccacgaa cccgctcaag atccaggacg tgacgttcag ggacgctcac cagtccatct
     1921 tcgccacgcg cgtccgcaca caagacctcc tggtgatcgc cgagaagatg gacagggtgg
     1981 gtttccactc gatggaggtc tggggaggcg ccaccttcga caccatgcat aggttcctcg
     2041 gggaggaccc gtgggaacgg atcagggtcc tgaagaggca catgggcagg acaccgttct
     2101 ccatgctgct caggggacag aatctcgtgg ggtacaggaa ctacccggac gacgtggccg
     2161 aggccttcgt ggagcgctgc tgcatcaacg ggatcgacat cttcaggata ttcgacgcgc
     2221 tcaacgattt ccgcaacttc gagaccgtcg tgagggtggt caaggaacac ggcaagcact
     2281 tccagggcgc catctgctac tcgctcaccg agaggcgcat gggcggcgag gtctacacgc
     2341 tcgaatacta cctcgacaag gcgaa
//
LOCUS       DAOSCR010000448         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29617_length_2355_cov_3.432174, whole genome shotgun sequence.
ACCESSION   DAOSCR010000448 DAOSCR010000000
VERSION     DAOSCR010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29617_length_2355_cov_3.432174"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    713..722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<723..>2355)
                     /locus_tag="PLT69_10180"
     CDS             complement(<723..>2355)
                     /locus_tag="PLT69_10180"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOJ14848.1"
                     /translation="VRRTVIGFDIERAGIWKKIGVLMRIRSEASRVASEKPDLVLTIG
                     TPATKYAKDTIIDAGVPLVFTAVAIPEAAGCESLTKAGKGFTGSTLHMDMPRALGRLK
                     KAFPNARVVGMVHSDDENGVAHVEEARRFCEDAGLTLVSREVDKNESITEALEELAAK
                     GVQVFCVPLDTYYGLRNYEACRALEVFSNRTRIPVVSFALMHVPGAVLYIGSDFRRIG
                     ELSGGQALEILLKGKKPTELPILMQPEPAVWVDQGRTKALGLALPGEITGSAQVIDLS
                     RDRKYRIEVLQVTDIAPFQDAYEGFMRVLKENGMEEGKNLVVGRKIIDFDLEKAGLWD
                     RIGVLARIRAEARRIAELKPDLVLTIGTPATRYARSAIVDAGIPLVFTAVAIPEAAGC
                     PSLTEGGKGVTGSTLYMNMHNALKIVRLAFPGIKKVGVIHSDDENGIAHAEEAERYGP
                     ADVGITFIRQEVTKNDSITPAAEKLAKEGVEAFCVPLDTYYALRDYEAAKEFREFSLK
                     TRIPIFSFALVKMPGAMMYVGSDFKTVGSLAGTQAV"
BASE COUNT          362 a          814 c          731 g          438 t
ORIGIN      
        1 atcggcctca gaccttatcg gctcgcgcca aagaggtctg tcgtcggaaa gggcttcaag
       61 gggcatccgc tccatcgggg tgaacaacgg cgtaccaggc gctccacatg ggtctccgag
      121 tactacgcct cgagcgacac ccgcgccatc ggcctcagac cttatcggct cgcgccaaag
      181 aggcctgtag ccggaaaggg cctcaagggg cgcccgcttc atcggggtga accaccccgc
      241 aagggggctc gcgctgcctg cctgcccggt cgggcgccat ggggccctgc aggggaccag
      301 gccggcctca ccacggggct gaccccctca ggcggagggt ttcaccccga ccatcaccat
      361 ctctagcggg gaagcgacac tgactccctc aggcgcaggg gttcacccgc actcccctcc
      421 cagggttggt ggcccggctg aacgtaccgt tgcgaggaac acttccgtgg ggatgccgat
      481 ggagggcggc caagggtcct ggcgccgccg cgagaggggc cccttggagc tcggcttctg
      541 gcctgcccgg acggcgtgcg cctgctcact gcacctcttc ggcgatctgg aggacctcga
      601 gcggcagcct gtaggccagc tccttgagct ggtacgtgtc gaccaggata cgcagctcct
      661 gttgctggag gataggcagg gcctcgggcc tcgccccttc cttgacgatc ttnnnnnnnn
      721 nntgaccgcc tgggtgccgg cgagcgagcc cacggtcttg aagtcggacc ctacgtacat
      781 catggcgccg ggcatcttca cgagggcgaa gctgaagatg gggatccggg tcttgaggga
      841 gaattccctg aactctttcg ccgcctcgta atccctgagc gcatagtacg tgtccaaggg
      901 cacgcagaac gcctcgaccc cctctttggc caatttctcg gccgcgggcg ttatgctgtc
      961 gttcttggtc acttcttgcc tgatgaaggt gatgcccacg tcagccggcc cgtacctctc
     1021 ggcctcttcc gcgtgcgcga tcccgttctc gtcgtcgctg tggatcacgc ccaccttctt
     1081 gatccccggg aaggcgagcc tcacgatctt gagcgcgttg tgcatgttca tgtagagggt
     1141 ggagcccgtc acccccttgc cgccctcggt gagcgagggg cagccggccg cctccggtat
     1201 ggccacggcc gtgaacacga gcggtatgcc cgcgtcgacg atcgccgagc gggcgtacct
     1261 cgtcgcaggc gtcccaatgg tgagcaccaa atccggcttc aactcggcga tgcgcctcgc
     1321 ctcggccctg atcctcgcga gcacgccgat cctgtcccac agcccggcct tctcgaggtc
     1381 gaagtctatg atcttcctgc cgaccacgag gttcttgccc tcctccatcc cgttctcctt
     1441 gaggacgcgc atgaaccctt cgtatgcgtc ctggaacggg gcgatgtccg tcacctggag
     1501 cacctctatg cggtacttgc ggtccctcga gaggtctatc acctgggccg atcccgtgat
     1561 ctccccgggc agagcaaggc cgagcgcctt cgtcctgccc tggtcgaccc acacggccgg
     1621 ctcgggctgc atgaggatgg gcagctctgt cggctttttc cccttcagga gaatctccag
     1681 ggcctggccg ccagagagct cgccgatcct cctgaagtca gaccctatgt agagcacggc
     1741 cccgggcaca tgcatgagcg cgaagctcac caccgggata cgggtcctgt tggagaagac
     1801 ctcgagggcc cggcacgcct cgtagttcct gaggccgtag taagtgtcca ggggcacgca
     1861 gaacacctgc acgcctttgg ccgcgagttc ctcgagcgcc tcggttatgc tctcgttctt
     1921 gtccacctcc ctcgagacga gggtgagccc cgcgtcctcg caaaacctcc gcgcctcctc
     1981 cacgtgcgcg acgccgttct cgtcgtcgct gtgcaccatc cccacgacgc gcgcgttcgg
     2041 gaaggccttc ttcagcctgc ccagcgccct gggcatgtcc atgtggaggg tggagccggt
     2101 gaaccccttg cccgccttcg tgagggactc gcaccccgca gcctcgggga tcgccacggc
     2161 ggtgaacacg agggggacgc ccgcgtcgat gatcgtgtcc ttggcgtact tcgtggcggg
     2221 cgtgccgatg gtcaggacga ggtccggctt ttccgaggcg acccgcgagg cctcggaacg
     2281 tatgcgcatg agcacgccga tcttcttcca gatacccgcc cgctcgatgt cgaaaccgat
     2341 gaccgtgcgc ctgac
//
LOCUS       DAOSCR010000449         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_29998_length_2334_cov_2.271172, whole genome shotgun sequence.
ACCESSION   DAOSCR010000449 DAOSCR010000000
VERSION     DAOSCR010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29998_length_2334_cov_2.271172"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLT69_10185"
     CDS             complement(<1..275)
                     /locus_tag="PLT69_10185"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOJ14849.1"
                     /translation="MGSHDIDDYTYTLLREEPFGGEDCYVIEALPKKVEFYSKVVIWI
                     RKGNFVPARADLYDPKGQFEKQGIFKDVKNIQGINTPTRIEMHNKING"
     gene            complement(257..694)
                     /locus_tag="PLT69_10190"
     CDS             complement(257..694)
                     /locus_tag="PLT69_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOJ14850.1"
                     /translation="MMFRGFAAAVLLAVGLVLMSQASAHAAGMTAYQIVKKSEDLLDQ
                     AKDSKADMFMVLVNSKGERRERTLNAYVKRYGNNKSKSIVFFRSPADVKGTSFLVWTD
                     ENKKDQQWLYLPALQRVRQIARAARARASWAPSSPSTTWGATT"
     gene            complement(691..>1120)
                     /locus_tag="PLT69_10195"
     CDS             complement(691..>1120)
                     /locus_tag="PLT69_10195"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ14851.1"
                     /translation="DYSIHLLNRYRHERRYGQSVEEALSTAIHTSGRGIVYNALAVAA
                     GFLVLAPSQFVIISQMGILVAVDMLTIAVSALTFLPACIMLLPPRLEAEPVTVPTGKP
                     VLALVTKGLPKRDHAVVRYDEEEEEGPGQGQEKLVREVQQ"
     assembly_gap    1121..1130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1131..>2047)
                     /locus_tag="PLT69_10200"
     CDS             complement(<1131..>2047)
                     /locus_tag="PLT69_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14852.1"
                     /translation="EARGREIVPHHHTRRDLVGPLLHWFSRVSIKHSRLVIVLSFVVL
                     AVSIFFGRHVKSDLNLIEDFRERSPIRTADRILNEKFGGTSLFQVVFETKGQDDIKDP
                     AVLRDMERLQARLMDIDQVGKAVSIVDFVKRMNQAMHDGDPAFYTIPESRELVAQYLL
                     LFSFSGGGEDLDSFVDYNYQKGQILLQMKTQSGYLASEVEEVVRRFNGSEGKAERLEN
                     VFTTGLAMLAKEFNRVVVTSQVRSFIFSFVLCFVITAGVFRSLRLGVYSMMPLIVPIA
                     LDFAIMGVSGIRLNAATATVASIDIGMGI"
     assembly_gap    2048..2057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2058..>2334)
                     /locus_tag="PLT69_10205"
     CDS             complement(<2058..>2334)
                     /locus_tag="PLT69_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ14853.1"
                     /translation="TALPVALMACGSAYGIHVVENVMADSFAGMRGKGGIDNAVARIR
                     IPVFMAALTTMAAFISLCTTPIVPLTHFGLLSAFGIFTAMGLALTFVP"
BASE COUNT          409 a          717 c          721 g          467 t
ORIGIN      
        1 ccgttgatct tgttgtgcat ctcgatgcgg gtgggggtgt tgatcccctg gatgttcttc
       61 acgtccttga agatcccctg cttctcgaac tggcccttcg ggtcgtacag gtcggcccgg
      121 gcgggaacga agttgccctt gcgtatccag atcacgacct tggagtagaa ctcgactttc
      181 ttcggtaacg cctcgatcac gtaacaatcc tcacccccga agggctcctc cctcagcagg
      241 gtgtaggtgt aatcgtctat gtcgtggctc cccatgtcgt agaaggtgaa ctaggtgccc
      301 atgaagctct cgcccttgcc gctcgcgcta tctgcctcac ccgctgcaac gccggcaggt
      361 agagccactg ctggtccttc ttgttctcgt ccgtccacac gaggaagctg gtgcccttga
      421 catcggccgg cgagcggaag aagacgatgg acttggactt gttgttcccg tagcgcttca
      481 cgtacgcgtt cagggtgcgt tcgcgcctct cacccttgct gttgacgagg accatgaaca
      541 tgtcggcctt gctgtccttg gcctggtcga gcaggtcttc ggacttcttg acgatctggt
      601 acgcggtcat cccggcggcg tgggccgatg cctgggacat gaggacgaga cctacggcga
      661 ggagcaccgc ggccgcaaaa cccctgaaca tcattgctga acctcccgta cgagtttttc
      721 ctgcccttgg ccgggccctt cttcctcttc ttcgtcgtag cgtacaacgg catggtccct
      781 cttgggaagc cctttcgtca cgagggcgag cacgggcttg cctgtcggca ccgtcacggg
      841 ttccgcctcg agcctgggag gcagcagcat gatgcaggca ggcaggaagg tcagggccga
      901 taccgcgatg gtgagcatgt ccaccgcgac caggatcccc atctgcgaga tgatcacgaa
      961 ctgcgaaggc gccaggacaa ggaacccggc ggccacggcg agggcgttgt agacgatgcc
     1021 ccttcccgag gtgtggatgg ccgtcgagag ggcctcctcg acgctttggc cgtaacgcct
     1081 ctcgtgcctg tagcggttga ggaggtggat cgagtagtcg nnnnnnnnnn cgatccccat
     1141 gccgatgtcg atgctggcga ccgtcgcggt tgcggcgttc agcctgatgc ccgagacacc
     1201 catgatggcg aagtccaggg cgatgggcac gatgagcggc atcatgctgt agacccccag
     1261 cctgagagac ctgaataccc ctgccgtgat gacgaaacag agcacgaacg agaagatgaa
     1321 gctcctcacc tggctcgtca ccacgacgcg gttgaactcc ttggcgagca tggcgagtcc
     1381 cgtggtgaac acgttctcga gcctttccgc cttgccttca gagccgttga accgcctcac
     1441 cacctcctcc acctcgcttg cgaggtaccc gctctgggtc ttcatctgga ggaggatctg
     1501 ccctttctgg tagttatagt cgacgaagct gtcgagatcc tcccccccgc ctgagaacga
     1561 gaagaggagg aggtactggg ccacgagttc cctggactcc ggtatggtgt agaaggccgg
     1621 gtcgccgtcg tgcatggcct ggttcatgcg cttgacgaag tccacgatgg acacggcctt
     1681 gcccacctgg tcgatgtcca tgagccgggc ctgcagcctc tccatgtccc tgaggaccgc
     1741 agggtccttg atgtcgtcct ggccctttgt ctcgaacacc acctggaaga gcgaagtccc
     1801 gccgaacttc tcgttgagga tcctgtccgc ggtcctgatg gggcttcgct cccggaagtc
     1861 ctcgatgagg ttgaggtcgc tcttgacgtg cctgccgaag aagatcgaca ccgcgagcac
     1921 gacgaagctc agcacgatca cgagcctgct gtgcttgatg ctgaccctgg aaaaccagtg
     1981 gaggagaggg ccgaccaggt cccgtctcgt gtggtgatgg gggacgatct cacgtccccg
     2041 cgcctcgnnn nnnnnnnggc acgaaggtga gggccaggcc catggccgtg aagatgccga
     2101 acgccgagag gaggccgaag tgggtcaagg gcacgatggg cgtcgtgcac agggagatga
     2161 aagccgccat ggtggtgagc gcggccatga agaccgggat cctgatcctc gcgaccgcgt
     2221 tgtctattcc ccccttcccc ctcatgcccg cgaaggagtc cgccatgacg ttctccacca
     2281 cgtggatgcc gtaggccgat ccgcacgcca tgagggcgac cggcagggcc gtcg
//
LOCUS       DAOSCR010000450         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30027_length_2332_cov_2.777778, whole genome shotgun sequence.
ACCESSION   DAOSCR010000450 DAOSCR010000000
VERSION     DAOSCR010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30027_length_2332_cov_2.777778"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PLT69_10210"
     CDS             complement(<1..1306)
                     /locus_tag="PLT69_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ14854.1"
                     /translation="MAKFKETSMGAIFQNRVEEYGDKTLVIWKNKGKWEEISWNKMNE
                     MVRDLGLFLINRGIQPGDKVALFSPNRYEWWVADLAIISIGAVNVPIYATNSAEESRY
                     IIENSDSKMCFVGTREHMDKILSVKSKLPNLGEIIIFDDIENPPQGVITLKDAYKEGK
                     AYKNKDDFDKRIPPINIDDVATIIYTSGTTGNPKGVMLTHKNFVSNVQQAENVDPDFL
                     KGDHTFLSFLPLAHSLERTVGYYAPIYLGKKVAFAESVDKLLENFQEIRPTFLVSVPR
                     IYERVHAGITAKVATAPPVKKALFNWAMGVARENLPYICEDKPRKGWFAFKYRIADKL
                     IFSKLRAALGMDRLVAAISGGGPLSVSDAEFFLGMGIKVLEGYGLTETTPIVSANKPK
                     LIKAGTVGPAVRDTIIKIGEDGEVLIKGPQVMKGYYKNPEATK"
     gene            complement(2082..>2332)
                     /locus_tag="PLT69_10215"
     CDS             complement(2082..>2332)
                     /locus_tag="PLT69_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ14855.1"
                     /translation="NGVEFSNRKQLVENQKVKDLYAREIEENMKDYARVEQIRKFALL
                     DKEWSQETDELTPTLKLKRRVINQKYASIIESLYPPGD"
BASE COUNT          395 a          672 c          751 g          514 t
ORIGIN      
        1 ccttggtggc ctcggggttc ttgtagtagc ccttcatgac ctgcgggccc ttgatcagga
       61 cctctccgtc ctcgccgatc ttgatgatgg tgtctctcac cgcggggccc acggttcccg
      121 ccttgatgag cttgggcttg ttcgcgctca cgatcggggt cgtctcggtg aggccgtagc
      181 cttcgaggac cttgatgccc atgcccagga aaaattccgc gtcggacacg gacagcgggc
      241 cgccgccgga gatggccgcc acgagcctgt ccatgccgag ggccgccctg agcttgctga
      301 agatgagctt gtccgcgatc ctgtacttga aggcgaacca tcccttccgg ggcttgtcct
      361 cgcagatgta cggcaggttc tcgcgggcga cgcccatggc ccagttgaac aacgccttct
      421 tcacgggcgg ggcggtcgcg accttggccg tgatgcccgc gtgtaccctc tcgtagatac
      481 ggggcacgct caccaggaag gtgggcctga tctcctggaa gttctccagc agcttgtcca
      541 cgctctcggc gaacgccacc ttcttgccga ggtagatggg agcgtagtac ccgaccgtgc
      601 gctcgaggga gtgcgccagg ggcaggaagg acaggaaggt atggtcaccc ttgaggaagt
      661 cggggtccac gttctcggcc tgctgcacgt tcgagacgaa gttcttgtgg gtgagcatga
      721 cgcccttggg gttgcccgtg gtgcccgagg tgtagatgat ggtcgccacg tcgtcgatat
      781 tgatgggcgg gatcctcttg tcgaagtcgt ccttgttctt gtaggccttt ccttccttgt
      841 aggcgtcctt gagggtgatg actccctgcg gcgggttctc tatgtcgtcg aagatgatga
      901 tctcgccgag gttcgggagc ttggacttga ccgagaggat cttgtccatg tgttcccggg
      961 tacccacgaa gcacatcttg gagtcggagt tctcgatgat gtaccgggat tcctcggcgg
     1021 agttcgtggc gtaaatgggc acgttcaccg caccgatgga aatgatggcg aggtcggcca
     1081 cccaccactc gtagcggttc ggagagaaga gcgccacctt atcgccgggc tggatccccc
     1141 tgttgatgag gaacagaccg agatcccgaa ccatttcgtt catcttgttc caagagattt
     1201 cctcccactt gcccttgttc ttccagatga ccagcgtctt gtcgccgtac tcctcgacgc
     1261 ggttctggaa gatggcaccc atggatgttt ccttgaactt ggccatcgat aaccctcctc
     1321 atactatttt cgaatcatta tgctgccagg tgattgatgc atgataatgg attgcattga
     1381 aattatcaat agcgatcgtg agggatcgtg acataagacg accccgggtc gcggcagagg
     1441 tgaggaaggt cggtttcaag gcctggcggg cttggggccg cgatgcggtc atgaaggaca
     1501 agggtgaggc atctgtcatg gtgggggcgg cgccgagagc tgtgcctcgt tgtccggaac
     1561 aagccgggaa ggtgaccgga accagggggt gatccatcgg gcggatggag ggactatctc
     1621 agccatcgtc ttcagcacgg cgcaaaggat gatccctcag gcggatggag gggcacaaag
     1681 ctcatgggaa gtcgcacatc cccgccaccg tgtccgggga catctgctca aggccctcgt
     1741 cttcgcgccc gttgcgatgg ttatgggatc cctgtctctt ggccgagcag ggcaggagcc
     1801 gtgttcgagc atggacggac gtgccggttc gcaccctctg ccgcaaccca tgggcggggg
     1861 tgtcggcgca ggccgaaaaa aggcgggatg atctgcgggt gacacccttc gccctttggc
     1921 gaaggcaggg tctgcgcaac ccacacgggc ccgtgatgcg ggtccctcag gtccactacg
     1981 agccctccgg aggcgcgcaa accacacggg cccgtgatgc ggcctcgacc tttgtgggcg
     2041 cagggcaggg tgtcaggccc cgcgcccacg aagggttcct ttcagtcgcc cgggggatag
     2101 aggctctcga tgatggaagc gtatttctgg ttgatgaccc tgcgcttgag cttgagcgtg
     2161 ggggtcagct cgtccgtctc ctgggaccac tccttgtcga ggagtgcgaa cttgcggatc
     2221 tgctcgaccc gagcgtagtc cttcatgttc tcctctatct cgcgcgcgta gaggtccttc
     2281 accttctggt tctcgacgag ctgcttgcgg ttcgagaact ccaccccgtt ct
//
LOCUS       DAOSCR010000451         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30144_length_2326_cov_3.243065, whole genome shotgun sequence.
ACCESSION   DAOSCR010000451 DAOSCR010000000
VERSION     DAOSCR010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30144_length_2326_cov_3.243065"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLT69_10220"
     CDS             complement(<1..1345)
                     /locus_tag="PLT69_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOJ14856.1"
                     /translation="MVAYESPLVIAKAQSEIAIIPRMANRHGLIAGATGTGKTVTLRV
                     MAERFSAIGVPVFMADVKGDLSALCSAGGDNPKVRERVAQLGLDGFTYEGFPVVFWDV
                     FGEQGHPVRTTVSEMGPVLLGRILMLNDIQGGVLTLTFRIADDNGLLLLDLKDLRAML
                     QFVGDNAEQFKTQYGNISAASIGAIQRGLLALEEQGGDRFFGEPALNLQDLMQTDAGG
                     KGVINIFTADRLMASPKLYATFLLWMLSELFETLPEVGDPDKPRLVFFFDEAHLLFDD
                     APKALVEKIEQVVRLIRSKGVGVYFITQNPLDLPETVLGQLGNRVQHALRAFTPKDQK
                     NVKAAAETFRAGPGMDVTKAITELGVGEALVSVLDEKGAPTEVQRAFICPPQSRLTPL
                     SPEERQDVIRKSPCWGIYEKAVDRESAFEMLKARSEQAAREREEILEQKREANDAR"
     gene            complement(1793..>2326)
                     /locus_tag="PLT69_10225"
     CDS             complement(1793..>2326)
                     /locus_tag="PLT69_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ14857.1"
                     /translation="KIICHAAGAHHLMPEARTVIDIGGQDSKAMAVDHKGAVRDFVMN
                     DKCAAGTGRFLEVMARALEVNLDDFGNQSLLAPAPAKISSICTVFAESEVISLISRGE
                     PRENIIAGIHDAVAARVYAMAQRIKADPPIMMTGGVAKNQGVVKALERRFGSPISTHE
                     LAQVNGAIGAAVLASGI"
BASE COUNT          376 a          780 c          739 g          431 t
ORIGIN      
        1 tcctcgcatc gttggcttcc cgcttctgtt cgaggatctc ttccctctcc ctggccgcct
       61 gctcggagcg ggccttgagc atctcgaagg ccgattcccg gtcaacggcc ttctcgtaga
      121 tgccccagca gggcgacttc ctgatcacgt cctgccgttc ctcagggctc aggggggtga
      181 gcctgctttg gggcgggcat atgaaggcgc gttgcacctc ggtgggcgcg cccttctcgt
      241 cgaggaccga gaccagcgcc tctcccaccc ccagctcggt gatcgctttg gtcacgtcca
      301 tgcctggtcc cgcccggaag gtctcggcag cggccttcac gttcttctgg tccttgggcg
      361 tgaaggcccg cagggcgtgc tggaccctgt tccccagctg gccgagcacg gtctcgggca
      421 ggtccagggg gttctgggtg atgaagtaga cgcccacacc cttggagcgg atgagccgca
      481 ccacctgctc gatcttttcc accaaggcct tgggtgcgtc gtcgaagagg aggtgggcct
      541 cgtcgaagaa gaacaccagc cttggcttgt ccgggtcgcc aacctcgggg agcgtctcga
      601 agagctcgga gagcatccac agcaggaagg tggcatagag cttgggtgag gccatgagcc
      661 tgtccgcggt gaagatgttg atgacgccct tcccgccggc atccgtctgc atgaggtcct
      721 gcaggttcag ggccggctcg ccgaagaacc tgtccccgcc ctgctcctcc agggccagga
      781 ggccccgctg gatggccccg atgctggcgg cggagatatt cccgtactgg gtcttgaact
      841 gctcggcatt gtccccaacg aactggagca tggcgcgcag gtccttcagg tcgaggagga
      901 gcaggccgtt gtcgtccgca atcctgaacg tgagggtcag gaccccgccc tggatgtcgt
      961 tgagcatgag gatacggccc aggagcacgg gccccatctc ggagaccgtg gtgcggaccg
     1021 ggtgcccctg ctcgccgaag acatcccaga agaccacggg gaagccctcg taagtgaacc
     1081 cgtcgaggcc gagctgcgcc acgcgctccc tcaccttggg attgtccccg cctgcggagc
     1141 acaacgcgga gagatcgccc ttgacgtccg ccatgaagac cgggaccccg atggcgctga
     1201 accgctccgc catgacccgc agcgtgacgg tcttgcccgt gcccgtggcc ccggcgatga
     1261 ggccgtgccg gttggccatc ctcgggatga tggcgatctc ggactgggcc ttggcaatga
     1321 ccaggggact ttcgtaggcg accatgacac ctccctcacc atttcatgga aaagaacata
     1381 atacaggcga tctcggacgt gtgtcaacac gggcatgtcc ccgccatgac ccctggtcaa
     1441 ggacccaccg ggagggccat caccgccctt gcgtgccgcg acccgagccc cttggggtcc
     1501 gtggtgagaa ccaaagacga aaggcttttg gtgcctcttg tcccggttca gtcaggcctc
     1561 gaggtggacc cgtccccaaa ataccttctg aagctcatcc gcaacccata tacccaggta
     1621 caccaggaga ccgagaacag gccatccgga agagcgcctc tcccgcggag ttctcccccc
     1681 tcatcgggaa caaggacctc gaggaactcg atccctcaag cgtccggctg gacgacgacc
     1741 cgccgaacgc ttttctcccg tcggacgttt cgggcagggc ggcctgcgga ggtcagatgc
     1801 ctgatgcgag cacggcggcg cctatggcgc cgttgacctg ggcgagctcg tgcgtgctga
     1861 tcggcgagcc gaacctccgt tccagggcct tgaccacccc ttggttcttg gccacgccgc
     1921 cggtcatcat gatcgggggg tcggccttga tgcgctgggc catggcgtag accctggccg
     1981 ccaccgcgtc gtggatcccc gcgatgatgt tctcccgcgg ctcccccctc gagatgaggg
     2041 agatcacctc cgactcggcg aacacggtgc agatgctgct gatctttgcc ggcgcagggg
     2101 cgaggagtga ctggttcccg aagtcgtcca ggttcacctc cagggccctc gccatgacct
     2161 cgaggaagcg tcccgtcccg gcggcgcact tgtcgttcat gacgaagtcc ctcaccgcgc
     2221 ccttgtgatc gacggccatg gccttgctgt cctggccgcc gatgtcgatc accgtcctcg
     2281 cctcgggcat gaggtggtgt gcgcccgcgg cgtggcagat gatctt
//
LOCUS       DAOSCR010000452         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30182_length_2324_cov_2.934773, whole genome shotgun sequence.
ACCESSION   DAOSCR010000452 DAOSCR010000000
VERSION     DAOSCR010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30182_length_2324_cov_2.934773"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..387)
                     /locus_tag="PLT69_10230"
     CDS             complement(196..387)
                     /locus_tag="PLT69_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14858.1"
                     /translation="MGNRTAGTLDRALAWVCVHCPVCTYARKTQKGALFAFVRGIESR
                     ICPFCMAYERVYGRKAHEG"
     gene            complement(777..1268)
                     /locus_tag="PLT69_10235"
     CDS             complement(777..1268)
                     /locus_tag="PLT69_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOJ14859.1"
                     /translation="MKRQVYPVTPAVRVLREKGIPFAPHSYPYVENGGTHQVALSLHI
                     PEHRVIKTLVMKDEESRPFIVLMHGDREVSTRSLARQLGLRHVEPCSERDAQRLTGYL
                     VGGISPFGTKTELPVYAEAGIFDMDRIFINGGKRGFMVEIDPAHLEKALKVTRVYAAV
                     ENR"
     gene            1429..1638
                     /locus_tag="PLT69_10240"
     CDS             1429..1638
                     /locus_tag="PLT69_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ14860.1"
                     /translation="MPIYEYKCNACGHEFEALVPMSAKKAPSCPGCGSAKVKKLISVI
                     ASNTSGCSSCTSSSCSSCSPKGGFS"
     gene            complement(1698..>2324)
                     /locus_tag="PLT69_10245"
     CDS             complement(1698..>2324)
                     /locus_tag="PLT69_10245"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOJ14861.1"
                     /translation="AQGANLDMDHGTYPYVTSSNTIAGQACVGTGIGPTAIDMVVGIC
                     KAYTTRVGGGPFPTELKDEIGERLRAFGGEYGATTGRPRRCGWLDLVSLRNSMRLSGI
                     SRLAITKLDVLSGLETLRVATAYRIDGRIVEDMPANLDMYPEVEVLYDELPGWEEPLS
                     DIRSLDALPKRCRDYISYLEDRLGVKTAIVSVGPRREETIVLEDIFPS"
BASE COUNT          358 a          792 c          737 g          437 t
ORIGIN      
        1 cctcgagcag atcttccaga tacacgtctc acccccttga aggtctttct tggtttgtct
       61 ccttccccct gcacggtacg tgcgcccctc tgtccgccct ggggcttcga gcccccggct
      121 ccccggcacg ggcggatccc ctctccggcc cgcaggcaca ccacaggtgc acgtccgtgg
      181 ggccaggccg agatctcacc cctcgtgcgc cttcctgccg tagacccgct cgtaggccat
      241 gcagaagggg cagatcctcg actcgatgcc cctgacgaag gcgaagagcg cccccttttg
      301 ggtctttctg gcataggtgc acaccgggca atgcacgcag acccatgcga gcgcccggtc
      361 cagggtgccg gccgtcctgt ttcccatggt tcgccccccc acgacggcca ctgccccagg
      421 atacggcgtc ccctgcacag gtgcttggcg gtccgccact gcctggggcc gtggtccgag
      481 atcacgctgc tccatgaacc atcctcaata actataagta ttctcggcgc gcgcgcaaga
      541 gcggggtcgg cgcggcgagc gccccaagca tgtcgagggg cctcgcaaac ggtcagcccc
      601 gctcgaccgg tgcggtgcag ggggccaaga tcttgggcca tcagcccgcc gggttttcag
      661 acgcccgagc ctgtgagggg gtgacgcctg gcccgtaatg gtgttcgacg aggtcccctg
      721 acgccctagc ctgagaggtg gtgatgccgg ggtccacggc ggtcccggag gctgcatcac
      781 cggttctcga ctgccgcgta gaccctcgtg accttcaagg ccttctccag gtgggctggg
      841 tctatctcca ccatgaagcc gcgcttgccg ccgttgatga agatgcggtc catgtcgaag
      901 atgcccgcct ctgcatagac gggcagctcc gtcttcgtgc cgaaggggct gatgcccccc
      961 accaggtagc cggtcaggcg ctgggcgtca cgctcgctgc acggctcgac gtgcctgagg
     1021 ccgagctggc gggcgagact cctggtcgag acctctctat ccccgtgcat gagcacgatg
     1081 aacggtctcg attcctcgtc cttcatgacg agcgtcttga tcacgcggtg ctcgggtatg
     1141 tggaggctga gcgccacctg gtgtgtgccg ccgttctcca cgtacgggta ggaatggggt
     1201 gcgaaaggga tccccttctc cctgagcacc ctcaccgccg gtgtcaccgg gtagacctgc
     1261 ctcttcaagc gggccccctc tcgcacaggc atgccctgtc tcgcccatgc ccgcgcacga
     1321 acctcgaagg cccgtgcatg ccccgggggt cttgacacgg ggcgggggag gtactacgaa
     1381 gatccttggg ccgcctcacg cggcctagcg tctggcaaag gagtcgacat gcccatctat
     1441 gagtacaagt gcaacgcatg cggccacgag ttcgaggccc tcgtgcccat gagcgcgaag
     1501 aaggcacctt cctgcccggg ttgcggcagc gcgaaggtga agaagctgat atccgtgatc
     1561 gcttcgaaca cttcgggatg ttcctcgtgc acgtcatcgt cgtgctcttc gtgttcgccc
     1621 aagggtggct tttcctgagg gtaggcctcc agaggcccgg tccgagcctt tcacacccct
     1681 ttcgatcgtg actgcagtca ggaggggaag atgtcctcga gcacgatcgt ctcttcccgt
     1741 ctcggcccca cggatacgat ggcggtcttg acgccaagac ggtcctccag gtacgagatg
     1801 tagtccctgc acctcttcgg cagcgcgtcc agggacctga tgtcgctcag gggttcctcc
     1861 cagccgggaa gctcgtcgta gagcacctcc acctcggggt acatgtcgag gttcgcgggc
     1921 atgtcctcta cgatgcggcc gtctatgcga taggcggtgg cgaccctcag ggtctcgagg
     1981 cccgacagga cgtcgagctt ggtgatggcg agcctggaga tgccgctcaa gcgcatggag
     2041 ttcctcaagg acacgaggtc gagccaaccg cagcgcctgg gcctgcccgt ggtggcgccg
     2101 tactcgccgc cgaacgccct gagcctctcg ccgatctcgt ccttcagctc cgtggggaag
     2161 ggcccgcccc cgacccgcgt ggtgtaggcc ttgcaaatgc ccacgaccat gtcgatcgcc
     2221 gtggggccga tgccggtgcc cacgcacgcc tggcccgcga tggtgttcga cgaggtcacg
     2281 taggggtacg tcccgtggtc catgtcgagg ttcgcgccct gggc
//
LOCUS       DAOSCR010000453         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30363_length_2315_cov_2.527876, whole genome shotgun sequence.
ACCESSION   DAOSCR010000453 DAOSCR010000000
VERSION     DAOSCR010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30363_length_2315_cov_2.527876"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLT69_10250"
     CDS             <1..182
                     /locus_tag="PLT69_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ14862.1"
                     /translation="EPDTSLIEAGIIDSTGVLELVEYLESTFGIKIEDEELVPENLET
                     ISNILRFLASKGVGA"
     gene            179..1714
                     /locus_tag="PLT69_10255"
     CDS             179..1714
                     /locus_tag="PLT69_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOJ14863.1"
                     /translation="MSGTVHDLLAASAARHPDKTAVFQGGRSATFAEIDSLANRVARL
                     LTQAGLDRQDRVVVIMGNSIESVGVYYGILKAGLISVEIHEKSTPSEVSYFIQNAGAR
                     ACVLAEAPASRLGGIEIPVLVSPAGPGSSRARTFVPWSALDAVADERFEARVSSGDIA
                     SIVYTSGSTGRPKGVVLTHGNIRSNTLAIVSYLGLQEDDRVMAILPFHYVYGKSLLNT
                     HMAVGAQVVINNRFAFPNAVIREMCERSVTGFAGVPSTFAILMNRSVFPKTRIPSLRY
                     VTQAGGAMAPALTRRLLDCLDGVDLYVMYGATEASARLTYVPPDRLRRDKLGSIGIPI
                     PGVEVTVRDDQGRELAPLEVGNICAQGPNIMQGYWNDPEETSRALKPWGLVTGDLGYR
                     DEEGFLFLTGRKKDMLKIGGERVSPKEIEDAIVESGMVHEAAVVGKPDQYLFEVPLAF
                     VVPLDEKTFDLEALRSFVAGRLSPHKHPRHWTVAKTLPKKPSGKIDKEALRHEALLEM
                     RVA"
     gene            1683..>2315
                     /locus_tag="PLT69_10260"
     CDS             1683..>2315
                     /locus_tag="PLT69_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14864.1"
                     /translation="MRHCSRCVLPETFPGISFDPAGVCNYCRNTPVPTPEEKREHLAR
                     FERLVEERKGTSAFDCILAYSGGKDSTYTLMMLRQTYGLRVLAFTFDNGFVSRQAMEN
                     IRAMTDRLGAVSMVFRPPFDLMRKAFSLSAVKLFYSPKTLDRASSICTTCIGMVKALV
                     LKTALAYRIPLVAYGWSPGQAPIASAIMQTGPRLQRISQATVKDPLVREVG"
BASE COUNT          431 a          766 c          736 g          382 t
ORIGIN      
        1 tggaaccgga cacctccctg atcgaggccg gcattatcga ttccacgggc gttctcgagc
       61 tggtggagta cctcgagtcg accttcggca tcaagatcga ggacgaggaa ctcgttccgg
      121 aaaacctgga gaccatctcc aacatcttga gattcctcgc ctcgaagggc gtgggcgcgt
      181 gagcggcacc gtacacgatc tcctcgccgc atccgcagcc cgccacccgg acaagacggc
      241 ggtcttccag ggcggtcgct cggccacctt cgcagagatc gactcgctcg cgaacagggt
      301 cgcacgcctg ctcacccagg ccgggctcga caggcaggac cgggtggtcg tgatcatggg
      361 aaactccata gagagcgtcg gggtgtacta cgggatcctg aaagccgggc tcatcagcgt
      421 ggagatccac gagaagtcga ccccgagcga ggtctcgtac ttcatccaga acgccggggc
      481 gcgggcatgc gtgctcgccg aggcaccggc atcgaggctc ggcggcatcg agatcccggt
      541 gcttgtaagc cctgccgggc ccgggtcgag ccgggcgagg acgttcgtgc cgtggtccgc
      601 cctcgacgcg gtcgcggacg agcggttcga ggcccgtgtc tcctcgggcg acatcgcgtc
      661 gatcgtgtac acgtccggga gcaccgggag accgaaaggc gtcgtcctca cgcacgggaa
      721 catccgttcc aacacccttg ccatcgtatc ctatctgggg ctccaggaag acgaccgggt
      781 catggcgatc ctgcccttcc actacgtcta cgggaagtcg ctcctgaaca cgcacatggc
      841 cgtgggcgca caggtggtca tcaacaacag gttcgcattc cccaacgccg tgatcaggga
      901 gatgtgcgag cgctcggtga cggggttcgc gggcgtcccc tccaccttcg ccatcctcat
      961 gaacaggtcg gtcttcccca agacccgcat tccgtcgctc cgctacgtca cccaggcggg
     1021 cggggccatg gccccggccc tgaccaggcg cctgctcgat tgcctcgacg gcgtggacct
     1081 ctacgtcatg tacggggcga ccgaggcatc agcccggctc acgtacgtcc cccccgacag
     1141 gctcaggagg gacaagctgg gatccatcgg gatacccatc cccggcgtgg aggtcacggt
     1201 gagggacgac cagggaaggg aactcgctcc gctcgaggtg ggaaacatct gcgcacaggg
     1261 tcccaacatc atgcaggggt actggaacga ccccgaagag acctcgcgcg cactcaagcc
     1321 ctggggcctc gtcaccggcg acctcgggta ccgggatgag gaaggattcc tcttcctcac
     1381 ggggaggaag aaggacatgc tcaagatcgg cggtgaacgg gtatccccga aggagatcga
     1441 ggacgcgatc gtcgaatccg gcatggtcca cgaggcggcg gtcgtgggca agcccgacca
     1501 gtacctcttt gaggtccctc tggcgttcgt ggtgcccctc gacgaaaaga ccttcgacct
     1561 cgaggcgttg aggtcgttcg tcgccgggag gctctcgccg cacaagcatc ccaggcattg
     1621 gaccgtcgcg aagaccctgc ccaagaaacc ctctgggaag atcgacaagg aggcactcag
     1681 acatgaggca ttgctcgaga tgcgtgttgc ctgagacctt tcccgggatc tccttcgatc
     1741 ctgcgggcgt gtgcaactac tgcaggaaca cccccgtgcc gacgcccgag gagaagcgcg
     1801 agcacctcgc gagattcgaa cgcctcgtcg aggagaggaa ggggacctcg gcctttgact
     1861 gcatccttgc ctacagcggg ggcaaggact ccacctacac gctcatgatg ctccggcaga
     1921 cctacgggct cagggtgctg gccttcacct tcgacaacgg gttcgtctcg aggcaggcga
     1981 tggagaacat ccgggccatg acggacaggc tcggagctgt gagcatggtc ttcaggccgc
     2041 ccttcgacct catgcggaag gccttctccc tctcggccgt gaagctcttc tacagcccca
     2101 agaccctgga cagggcgagt tccatctgca cgacgtgcat cggcatggtc aaggccctcg
     2161 ttctcaagac cgcgcttgcg taccgcatcc ccctcgtcgc ctacggctgg tcccccggcc
     2221 aggcgcccat agcctcggcg atcatgcaga ccggcccgag gctgcagcgc atctcccagg
     2281 ccacggtgaa ggacccgctc gtcagggagg tgggg
//
LOCUS       DAOSCR010000454         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30368_length_2314_cov_3.631695, whole genome shotgun sequence.
ACCESSION   DAOSCR010000454 DAOSCR010000000
VERSION     DAOSCR010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30368_length_2314_cov_3.631695"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..736
                     /locus_tag="PLT69_10265"
     CDS             2..736
                     /locus_tag="PLT69_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOJ14865.1"
                     /translation="MVKRHGLVVLIEHWAIAISGLALILTGLFELPIARRYFITEIPG
                     LRWSGDYIFSLWLHYAFSVVFVGASLFHVVYHGLMGHRAILPKRGDLRESWEVIKSFF
                     GKGEEPPFAKYLPEQRLAYAGIALIIAGLIASGLVKTWKNLFAPDMDLTLVLWATWAH
                     NLFFVLFVLAFVAHMAAILIKPNRPMIRAILTGWIRRDYAEHRHPLWIAELAGRNDHP
                     AREQEAPTRERPPDGEAGGENPQGLS"
     gene            807..2207
                     /locus_tag="PLT69_10270"
     CDS             807..2207
                     /locus_tag="PLT69_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOJ14866.1"
                     /translation="MARKKRVPSPATSTEAILESISDGVFTVDEHWVVTSFNRAASKI
                     TGIPRDEAVGRLCSEVFRSSMCEVDCALKRTLKTGRPVIGRSCFIIDAQGRRIPISVS
                     TAVLKDAKGNVIGGAETFRDLTELEELRQELEGRAHVGELVSRSPLMQKVFEVIPAVA
                     ASPSTVLILGETGTGKEIVARTIHSLSPRRDGPFIAVNCAALPDTLLESELFGYKAGA
                     FTGAARDKPGRFALAKGGTLFLDEIGEVSPALQVRLLRVLQDRKYEPLGATRSETADV
                     RVMVATNTDLARLVKQGSFREDLYYRVNVVRLELPPLRRRKEDIPLLVEQFVTRFNAL
                     QHKSVQGLTTEALSLLMAYDWPGNIRELANVIERAFILCEKGSIGVAHLPEEITARSL
                     QHAAQPGLKSAAQMLEAQAIRRALERCANNRLAAARELGIHKSTLFRKMKRLGIAPAG
                     TDGRAGRRVKRERRCD"
BASE COUNT          414 a          783 c          742 g          375 t
ORIGIN      
        1 cctggtcaag cgccacggcc tcgtcgtgct catcgagcac tgggccatcg cgatctccgg
       61 gctcgcactc atcctcaccg gtctcttcga gctccccatt gcaaggcgct acttcatcac
      121 cgagatcccg gggctcaggt ggtcgggcga ctacatcttc tcgctttggc tgcactacgc
      181 gttctccgtg gtcttcgtcg gcgcgagcct gttccacgtg gtctaccacg ggctcatggg
      241 ccaccgggcc atcctgccga agaggggcga cctcagggag tcgtgggagg tgattaagtc
      301 cttcttcggc aagggcgagg agccgccctt cgccaagtac ctcccggagc agcgcctcgc
      361 ctacgcgggc atcgccctca tcatcgccgg gctcatcgcg agcggcctgg tcaagacctg
      421 gaagaacctc ttcgcccctg acatggacct gacgctggtg ctctgggcca cctgggcgca
      481 caacctcttc ttcgtcctgt tcgtgctggc ctttgtcgcc cacatggcgg ccatcctgat
      541 caagcccaac cgtcccatga tccgtgccat cctcaccgga tggatccggc gtgactacgc
      601 cgagcaccgc caccccctgt ggatcgcgga gctcgccggg cggaacgatc atcccgctcg
      661 ggaacaggaa gccccaaccc gcgagaggcc gccggatggt gaagcgggtg gcgagaaccc
      721 gcaagggctt tcgtgaacag gcaggatgtg gtaatgcccc atgccgggcg cgcccgacat
      781 ggggcaagcc cccgaggagc gtaaccatgg cacgcaagaa acgggtcccc tctcccgcga
      841 catccacgga agccatcctg gagagcatct cggacggggt gttcacggtg gatgagcact
      901 gggtcgtcac ctccttcaac cgtgcggcct cgaagatcac cggcattccc cgggatgaag
      961 cggtgggcag gctctgttcc gaggtcttcc gctccagcat gtgcgaggtg gactgcgctt
     1021 tgaagagaac cctgaagacc ggcaggccgg tcatcggcag gtcctgtttc atcatagacg
     1081 cccaggggcg gcggatcccc atcagcgtgt ccacggccgt gctgaaggac gcgaagggga
     1141 acgtcatcgg gggggcggag accttccgcg acctcaccga gctcgaggag ctccgccagg
     1201 aactcgaggg acgggcccac gtgggcgagc tcgtgagcag gagccccctc atgcagaagg
     1261 tcttcgaggt gatccccgcc gtagcggcaa gccccagcac cgtgctcatc ctgggcgaga
     1321 ccggcacggg caaggagatc gtggccagga ccatccactc cttaagcccc aggcgggacg
     1381 gccccttcat cgcggtgaac tgcgccgccc ttcccgacac gctcctggag tccgagctct
     1441 tcggctacaa ggcgggggcc ttcaccggcg ccgccaggga caagccaggc aggttcgccc
     1501 tggcaaaagg cgggaccctc ttcctggacg agatcggcga ggtgagcccg gccctgcagg
     1561 tgcggctgct gcgggtcctc caggacagga agtacgagcc cctgggggcc acccggtcgg
     1621 agaccgccga tgtgcgggtc atggtggcca ccaacaccga cctggcaagg ctcgtgaagc
     1681 agggctcgtt ccgcgaggac ctctactacc gggtgaacgt ggtgcgcctg gagctgccgc
     1741 cgctgcgcag gcgcaaggaa gacatcccgc tcttggtcga gcagttcgtc acccgcttca
     1801 acgccctgca gcacaagtcc gtccaggggc tcaccaccga ggccctgtcc ttgctcatgg
     1861 cgtacgattg gcccggcaac atccgggagt tggccaatgt catcgagcgc gccttcatcc
     1921 tctgcgagaa gggctccatc ggcgtggccc acctgcccga ggagatcacc gcccgctccc
     1981 tgcagcacgc cgctcaaccc ggcctgaaat cggccgcgca gatgctcgag gcccaggcca
     2041 tccgcagggc gctggaacgt tgtgccaaca accgcctggc agcggcccgg gagctgggca
     2101 tccacaagag caccctgttc cggaagatga agaggctcgg catcgcacct gcaggcacgg
     2161 acggccgggc cggcaggcgg gtgaaaaggg agcgacgatg cgactgaagc ggtcatgaaa
     2221 ggttgcagaa gcgcgactct tcccgctggg aacccccttc ccttggtcca atttatttaa
     2281 caatatcgag cgaatgaatg catggggggt aagc
//
LOCUS       DAOSCR010000455         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30371_length_2314_cov_3.226206, whole genome shotgun sequence.
ACCESSION   DAOSCR010000455 DAOSCR010000000
VERSION     DAOSCR010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30371_length_2314_cov_3.226206"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="metG"
                     /locus_tag="PLT69_10275"
     CDS             <1..535
                     /gene="metG"
                     /locus_tag="PLT69_10275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOJ14867.1"
                     /translation="QFERQGYFCVDPKATSPGRPVFNRTVQLRDTWAKIEKASAGKAA
                     GPGSETAAEGKGASPSEGDHGVEAREITIEDFTKVDLRVGRVLEAHAVEGSDKLVRLL
                     VDLGEPRPRNIVAGIRKAYPEPVGLVGKLVLVVANLRPRKMRSGLSEGMILAAGSGDH
                     WRICTVDGDMSPGAKVS"
     gene            1371..>2314
                     /locus_tag="PLT69_10280"
     CDS             1371..>2314
                     /locus_tag="PLT69_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14868.1"
                     /translation="MKKRKCLEDLARLSDSLPDATFAIDTEGTVIIWNKAMASLTGMQ
                     AEEMIGRRQGAHAEAFYGERRPMLVDLLFTPDEQAEKQYDWVKREGQTITAQGPATVR
                     GAERYLHANASLLTDPSGSVIGAIETIRDITDWKKAELALRESEAKYRDIFLNVSDLV
                     YLHDLNGRFMETNLAAKKETGYTEKDLESLRVQDILPERHRDRFVNYLQNVVRKGRDE
                     GFFTIVTKDGTERIIEYRNSLVRDAYGRPTAVRGIARDITQRVTMTRRLRRERDLVKT
                     IIQTSPAFFGAVDVNGSVIMINQAMLEALGYREAEVLG"
BASE COUNT          484 a          717 c          769 g          344 t
ORIGIN      
        1 ccagttcgag cgccagggct acttctgcgt cgacccgaag gcaacgtcgc cgggcaggcc
       61 cgtcttcaac aggaccgtcc agctcaggga cacctgggcg aagatcgaga aggcctctgc
      121 cggcaaggcc gctggaccag gctcggaaac ggccgcggag ggcaagggcg cctctccctc
      181 ggaaggtgac catggcgtgg aggcccgcga gatcaccatc gaggacttca ccaaggtcga
      241 cctcagggtg gggcgcgtgc tcgaggcgca cgccgtggaa ggctcggaca agctggtgcg
      301 gctcctggtg gatctcgggg agccgagacc aaggaacatc gtcgctggca tacggaaggc
      361 ctatcccgag ccggtcgggc tcgtcgggaa gctcgtgctg gtggtcgcca acctcaggcc
      421 gaggaagatg aggtccggcc tgtcggaagg catgatcctg gccgcgggca gcggcgatca
      481 ctggcgcatc tgcacggtcg acggcgacat gtcgccggga gcgaaggtgt cctgaccggt
      541 gccgccttca aagcccttct agggcgagcc gccaggagat atggtgccgg gagagaagat
      601 gtcctgaccg cccgcgggga cccagcctcc tgcatgcacg cgcgcccggc gcccaggcct
      661 ccgggctgag atcccgcgca tgcttcacgc ggattctgcc attgccggta ccttcggcta
      721 tgacacgaag aacccgcgcc ctcttcatgc gtgttctgcc taccggtaag gcccgcatca
      781 ttgcatgacc tgacggctga gaacccgcgc ccactccacg cgggttctgc accgggcggc
      841 acggcacgga ccggccacgc atcgttccag gaagcggttg cattcccgcc gcctgttctt
      901 accttacggc gatgacaggc atccttgaca tcgtcaaggg cggccttatt ctccttattc
      961 aagaatcacc aagagacagc ccgggagcct cggccttgtc cataccgatg acgttcatcc
     1021 ttcccgcgac ggattccgat aacagaggtg tcggcatgca gtatgggacg aagagagacc
     1081 ggccgtcggc aggcccggac atgaacgcat gaagggttcg ggggagccct ttacccggat
     1141 gggctgcctt gcaaggcggc gcgagggatg cggcctggcc acgacagcag aaggccgccg
     1201 gagaaggatg cccggacctc gagccggcgc cggtgtcttg ggacagccaa gcggtcgtga
     1261 gagagcccgg aggttgtgag agcatgaagc ccaggggcaa aggcatacca gcagccaagg
     1321 tgacggcaaa gagatcggcg gcggacaccg cccgtgcggt ggagaagccc gtgaagaaga
     1381 ggaaatgcct cgaggacctg gcgaggctct ccgactctct gccggatgcc accttcgcga
     1441 tcgacaccga gggcacggtg atcatctgga acaaggcaat ggcgagtcta accggcatgc
     1501 aggccgaaga aatgatcggg aggagacagg gcgcccacgc ggaggcattc tacggcgaga
     1561 gacgccccat gctcgtcgac ctcctcttca cgcccgacga acaggcggag aagcagtacg
     1621 attgggtcaa gagggagggg caaacgatca cggcgcaggg gccggccacg gtgcggggcg
     1681 ccgaacggta cctccacgcc aatgcgtcac tcctgacaga cccttcggga agcgtcattg
     1741 gggccatcga gacgatacgg gacatcacgg actggaagaa ggcggaactc gccttgcggg
     1801 agagcgaggc gaaataccgg gacatcttcc tgaacgtatc cgacctcgtc tacctccacg
     1861 acttgaacgg caggttcatg gagaccaacc tcgccgccaa gaaggaaacg ggctacacgg
     1921 agaaagacct cgagagcctc agggtgcagg acatccttcc cgaacgccac agagaccgct
     1981 tcgtcaatta ccttcagaac gtcgtgagaa agggccgcga cgagggtttc ttcaccattg
     2041 tcaccaagga cggcaccgag cgcatcatcg aataccgcaa ctccctcgtg agggacgcct
     2101 acggcaggcc gacggccgtg cgcggcatcg cccgggacat cacgcaacgg gtcaccatga
     2161 ccaggaggct gcgcagggag cgggacctgg tcaaaaccat catccagacc tctcccgcgt
     2221 tcttcggcgc cgtagacgtg aacggctctg tgatcatgat caaccaggcc atgctggagg
     2281 cgctcggtta cagggaggcc gaggttttgg gaag
//
LOCUS       DAOSCR010000456         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30390_length_2313_cov_3.540301, whole genome shotgun sequence.
ACCESSION   DAOSCR010000456 DAOSCR010000000
VERSION     DAOSCR010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30390_length_2313_cov_3.540301"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLT69_10285"
     CDS             <1..846
                     /locus_tag="PLT69_10285"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ14869.1"
                     /translation="LGTLRSRGLTYEEDGALWLKTTAFGDEKDRVLRKKDGSLTYFAP
                     DIAYHKDKLARGFDWIIDIWGADHHGYVPRMKAAMSAIGAPEDCFHALLIQLVSLVRG
                     GAAVQMSTRAGEFVTLREIIDEVGKDAARFFFMMRRSDAQLVFDLDLAKKRSEDNPVF
                     YIQYAHARICSILKNAAELGIEPSCKEADLDVLSSGDDLELVKVLAACPDVVVDAALC
                     LEPHRIAFYLLDVASAFHRFYNRNRVISDDVTLRAARLVLVDAARQVIANTLALMGID
                     APETM"
     gene            852..1385
                     /locus_tag="PLT69_10290"
     CDS             852..1385
                     /locus_tag="PLT69_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOJ14870.1"
                     /translation="MGKKRKRRTGGPHKTTSTAKNVALLFLSLGMVVCSCIVYAYLFG
                     RHGITLDPLQKAISGLSSEERPDKPYPETGPTPPRQQAASAYDFMFYDMLGNKDEAHR
                     ETGSYSVQVAAFKSEESAKTMARSLEDKTRVKFRIDRKGPSYFVRWGSFSTKEKADRQ
                     CARLSEKLGRECIVVRM"
     gene            1525..>2313
                     /locus_tag="PLT69_10295"
     CDS             1525..>2313
                     /locus_tag="PLT69_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOJ14871.1"
                     /translation="MLHTGQHYDPSMSRAHFDALGLEEPHYHLGVGSGTHARQTARMM
                     EGIEDVLVSLRPSLVLVYGDTNSTLAASLSAAKLKIKVGHVEAGVRSFDRTMPEEINR
                     VVADHLSEHHFCPTAGALGLLEREGIKGLLTGDVMYDAILQSRPDTLVNPFPTPYALV
                     TIHRAENTDDEARFRGIWAGLELVSRELPVIFPVHPRTKAAHERLLACGTGRIRVIDP
                     VDYLLRHKTTARSRYDRALSGLQATPEVFDVIFCNARGEVCEGAR"
BASE COUNT          440 a          771 c          730 g          372 t
ORIGIN      
        1 ctcggcaccc tgagatccag ggggctcacc tacgaggagg acggggccct ctggctgaag
       61 acgacggcct tcggggacga gaaggacagg gtgttacgca agaaggacgg ttccctcacg
      121 tacttcgccc cggacatcgc ctaccacaag gacaagctcg cgcgcggctt cgactggatc
      181 atagacatct ggggtgcgga ccatcacggg tacgtccccc gcatgaaggc ggccatgtcg
      241 gccatcggcg cccccgagga ctgcttccac gccctcctca tccagctcgt ctccctcgtc
      301 aggggcgggg ccgcggttca gatgtcgacc agggccggcg agttcgtgac gctcagggag
      361 atcatcgacg aggtgggcaa ggacgcggcg agattcttct tcatgatgcg ccgctcggac
      421 gcgcagctcg tgttcgacct cgacctcgcg aagaagaggt ccgaggacaa ccccgtcttc
      481 tacatccagt acgcccacgc gaggatatgc tcgatcctca agaacgcggc cgagctcggc
      541 atcgagccct cctgcaagga ggcggacctc gacgtcctgt cctctggtga cgacctcgag
      601 ctcgtcaagg tcctggcggc gtgcccggac gtcgtcgtcg acgcggcgct gtgcctcgag
      661 ccccacagga ttgccttcta cctcctggat gttgcctccg ctttccatag gttctataat
      721 aggaaccgcg tgatatccga cgacgtgacg ctgcgcgcgg ccaggctcgt gctcgtggac
      781 gccgcgcgcc aggtcatcgc gaacacgctc gccctcatgg gcatagacgc acccgagacg
      841 atgtaggggt catgggaaag aagagaaaga ggcgcacagg cggcccacac aagaccacgt
      901 cgacggcgaa aaacgtggcg ctcctgttcc tttccctggg catggtcgtc tgctcctgca
      961 tcgtctacgc gtacctgttc ggacgtcacg gcatcaccct ggatccgctc cagaaggcca
     1021 taagcggctt gtcctcggag gagaggcccg acaagccgta cccggagacc gggcccactc
     1081 cgcccaggca gcaggcggcg agcgcctacg acttcatgtt ctacgacatg ctcggcaaca
     1141 aggacgaggc ccacagggag accgggtcct acagcgtcca ggtggcggcc ttcaagtccg
     1201 aggagagcgc gaagaccatg gcgaggtccc tggaagacaa gacgagggtc aagttcagga
     1261 tcgatcgcaa gggtccctcc tatttcgtaa ggtggggcag cttctccacc aaggagaagg
     1321 ccgacaggca gtgcgccagg ctctcggaaa aactcgggcg ggagtgcatc gtggtcagga
     1381 tgtaacgtac cgggggccgt ccttgtcccc ccggcggttg aggaggttgg agccataatc
     1441 ctgcacgttg tcggggcgcg gccccagttt atcaagctcg cccctgtcac cagggccttc
     1501 agggagctgg gtctgccgta caggatcctc cacaccgggc aacactacga cccctccatg
     1561 tccagggccc acttcgacgc cctcggcctc gaagaacccc actaccacct cggggtcggc
     1621 tccgggaccc atgccaggca gacggcccgc atgatggagg gcatcgagga tgtcctcgtc
     1681 tcgctcaggc cttccctggt gttggtctac ggggacacca actcgaccct ggcggcctcg
     1741 ctgagcgccg ccaagctcaa gatcaaggta ggtcacgtcg aggcgggcgt gaggagcttc
     1801 gacaggacca tgcccgagga gatcaaccgg gtcgttgcgg accacctgag cgaacaccac
     1861 ttctgcccca ccgcaggcgc cctcgggctg ctcgagaggg aagggatcaa gggcctgctc
     1921 acgggcgacg tgatgtacga cgccatcctc cagtcacgcc ccgataccct cgtgaacccc
     1981 ttcccgaccc cgtacgcgct cgtcaccatc caccgggcgg agaacacgga cgacgaggcc
     2041 cgcttcagag ggatctgggc agggctggaa ctcgtctcga gagaactacc ggtgatcttt
     2101 cccgtccacc cgcgcacgaa ggccgcccac gaaaggctcc tggcatgcgg aacgggacgc
     2161 atcagggtga tcgatcccgt cgactacctg ctgcgccaca agacgacggc gcgcagccgc
     2221 tacgatcggg cattgtccgg cctgcaggcg acgcccgagg tgttcgacgt catcttctgc
     2281 aacgcgcgcg gcgaggtctg cgaaggcgcg cgc
//
LOCUS       DAOSCR010000457         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30462_length_2310_cov_3.091796, whole genome shotgun sequence.
ACCESSION   DAOSCR010000457 DAOSCR010000000
VERSION     DAOSCR010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30462_length_2310_cov_3.091796"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLT69_10300"
     CDS             <1..513
                     /locus_tag="PLT69_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ14872.1"
                     /translation="ADCEVLSVAALLHDIGRISQDVSRGAVCHAEEGARMAREILSPF
                     DLPAEKKDNVIHCVLTHRFRNSHVPETLEARCLFDADKLDAIGAVGIARAYLFAGEVG
                     AVLHNPEASPEETLPYTRDDTGYREYMVKLRKIKDRMLTSEGRRMAQDRHAFMEAFFE
                     RFLKEYEGDV"
     gene            572..922
                     /gene="hypA"
                     /locus_tag="PLT69_10305"
     CDS             572..922
                     /gene="hypA"
                     /locus_tag="PLT69_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOJ14873.1"
                     /translation="MHELPVMQSILGIVLRHALMHGVKRVHSISLDVGELSDLEEEWM
                     QKYFDHLSRGTVAEGARLKVRWVKAVLVCDGCSTRFELDRNRSEPPACPGCGGTRLSL
                     ASGREYHIKDMEAE"
     gene            922..1590
                     /gene="hypB"
                     /locus_tag="PLT69_10310"
     CDS             922..1590
                     /gene="hypB"
                     /locus_tag="PLT69_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOJ14874.1"
                     /translation="MGEIRLIEVKEDILSDNKREAQELRGLLKQSKTFLLNLMSSPGA
                     GKTTLITRTIERLRDTFRMAVIEADIDSTVDAQKIAGLGIPAVQIRTGGFCHLDASMV
                     RKGLDALDLDRLDLVIIENVGNLVCPAEFDTGSIKNAMILSVPEGDDKPLKYPLMFTV
                     SHALVVNKIDYLGLSDFDMEALSIRVRALNRDIEIFEVSSKTGRGVDAWAGWLAEQVN
                     AFVA"
     gene            1677..>2310
                     /locus_tag="PLT69_10315"
     CDS             1677..>2310
                     /locus_tag="PLT69_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ14875.1"
                     /translation="MIRINENFLKLRPSYLFSEISRRLAAFQDSHKGASIIRLGIGDV
                     TRPLPRACIDAFHKAVDEMARQDTFRGYGPEQGYLFLREKIAEFDYRSRGADIDADEI
                     FISDGAKSDTANIQEIFSQDVRVAIPDPVYPVYLDTNVMAGRAGDMTDGRYSNIAYLD
                     ATPDNGFVPTPPDGQVDLVYLCYPNNPTGAVITKERLAQWVDYARRNKALI"
BASE COUNT          469 a          731 c          739 g          371 t
ORIGIN      
        1 gccgactgcg aggtcctctc ggtcgccgcc ctgctccacg atatcgggag gataagccag
       61 gatgtctccc ggggcgccgt gtgccatgcc gaggagggcg cgcgcatggc acgcgagatc
      121 ctctcgccct tcgacctgcc cgccgagaag aaggacaacg tcattcactg cgtcctgaca
      181 caccgcttcc ggaacagcca cgtgcccgaa accctcgagg ccaggtgcct gttcgacgcg
      241 gacaaactcg acgccatcgg cgccgtgggc atcgccaggg cgtacctctt cgcaggcgag
      301 gtcggcgccg tgctccacaa cccggaggcc tcccccgagg agacgttgcc ctacacgagg
      361 gacgacacgg gttaccgcga gtacatggtc aagctcagga agatcaagga ccgcatgctc
      421 acctccgagg gcaggcgcat ggcccaggac cgccatgcct tcatggaggc cttcttcgag
      481 aggttcctca aggaatacga gggggacgtc tgagtgcagg ccgcctttag ggtgtcaggc
      541 atccggggac cgctcgagga gggatgagct ggtgcacgag cttcccgtca tgcagagcat
      601 cctcggcatc gtgctcagac acgccctcat gcacggcgtg aagagggtcc actccatctc
      661 cctcgatgtc ggcgagctga gcgacctcga ggaggagtgg atgcagaagt acttcgacca
      721 cctgagcagg ggtaccgtgg ccgagggcgc caggttgaag gtgagatggg tcaaggcggt
      781 gctcgtgtgc gatgggtgtt ccacccggtt cgagctcgac aggaaccgca gtgaaccgcc
      841 cgcgtgcccg gggtgcgggg gcacgaggct ctcgctcgca tccggcaggg agtaccatat
      901 caaggacatg gaggccgagt gatgggagag atacggctga tcgaggtcaa ggaggacatc
      961 ctttccgaca acaagaggga ggcccaagag ctcaggggac tcttgaaaca gtcgaagacc
     1021 tttctcctca acctcatgtc ttctccgggc gcgggcaaga ccacgctcat cacgaggacc
     1081 atagagaggc tgagggacac cttccgcatg gccgtgatcg aggcggacat cgactcgacg
     1141 gtggacgcgc agaagatagc gggcctcggc atacccgccg tgcagatacg gaccgggggc
     1201 ttctgccacc tcgatgcatc catggtcaga aaggggctcg acgccctcga tcttgaccgg
     1261 ctcgacctcg tgatcatcga gaacgtgggc aacctggtgt gcccggcgga gttcgacacg
     1321 ggctcgatca agaacgcgat gatcctgagc gtgcccgagg gcgacgacaa gcccctcaag
     1381 taccccctca tgttcacggt gtcgcacgcg ctcgtggtga acaagatcga ttacctcggc
     1441 ctgtcggatt tcgacatgga ggccctctcc atacgcgtgc gcgccctgaa ccgggatatc
     1501 gagatattcg aggtctcctc gaagaccggc cggggtgtgg atgcgtgggc cggctggctc
     1561 gccgagcagg taaacgcctt tgttgcatga caccgaggcc ctgtgtaata gtgtccgccg
     1621 agttgcgccc cgtagcggcg catccgggtg gaaacaaggt cagacaaggg gcatgcatga
     1681 taaggatcaa cgaaaacttc ctcaagctga ggccgtcgta cctcttctct gagatatcga
     1741 ggcgcctggc ggccttccag gacagccaca agggggcgag catcatccgc ctgggcatcg
     1801 gcgacgtgac caggccgttg cccagggcat gcatcgacgc cttccacaag gccgtggacg
     1861 agatggcccg ccaggacacc ttcaggggct acggccccga gcagggatac ctcttcctca
     1921 gggagaagat cgccgagttc gactaccgct cgcgcggcgc cgacatagac gccgacgaga
     1981 tcttcatcag cgacggcgcg aagagcgaca ccgcgaacat ccaggagatc ttctcccagg
     2041 acgtgcgcgt tgccatcccg gatcccgtct acccggtata cctggacacc aacgtcatgg
     2101 ctggcagggc cggggacatg accgacggca ggtactcgaa catcgcctac ctcgatgcca
     2161 cgcccgacaa cggcttcgtg cccaccccgc ccgacgggca ggtggacctc gtgtacctct
     2221 gttaccccaa caacccgaca ggcgccgtca tcacgaaaga gaggctcgcc cagtgggtgg
     2281 actacgcgcg caggaacaag gcgctcatcc
//
LOCUS       DAOSCR010000458         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30489_length_2308_cov_3.924545, whole genome shotgun sequence.
ACCESSION   DAOSCR010000458 DAOSCR010000000
VERSION     DAOSCR010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30489_length_2308_cov_3.924545"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..813)
                     /locus_tag="PLT69_10320"
     CDS             complement(133..813)
                     /locus_tag="PLT69_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOJ14876.1"
                     /translation="MKIIEDAYRRGDKALNEYESKRLLKAYGIPVTEERVALTVEEAL
                     EAAHAIGYPVALKGSSRTLTHKTEHGLVELGIQGDDALEKAFAAITERGAGQLDGILV
                     QQMVKGDREFVAGLIRDPQFGPCVMFGLGGIFTEVLKDVTFRIAPLTTRDALEMMDEI
                     KARKLLDEFRGKPAVNREVLAATLVNLGKIGLELDQVAEIDINPMIVHGDIPVAVDAL
                     VVLRNPAA"
     gene            1338..1973
                     /locus_tag="PLT69_10325"
     CDS             1338..1973
                     /locus_tag="PLT69_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOJ14877.1"
                     /translation="MKLVVTEIFHSLQGEGPSIGTPSVFVRLGGCIEPLCPWCDTPYA
                     WHDFEEMDLEEIARRVASFGCRDVVITGGEPFLQWDVGLADLHASLRSSGFRIHYETS
                     GKAGIPSIDDAVVVASPKHIDGAWHIRRPDLANAHWFKFVADGEHSLRAIRSFVSENA
                     IDPEKVYVMPMGATRAQQMERMRPVFLFCRDNGLKMSPRLQVLVFDDERGV"
     gene            1976..>2308
                     /locus_tag="PLT69_10330"
     CDS             1976..>2308
                     /locus_tag="PLT69_10330"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOJ14878.1"
                     /translation="MEDAVILLSGGMDSGVLLAWAKERYREIHALTFNYGSKHAGRET
                     AMACRLAQHYGVTFFLAELPFVRELFRSSLLAGGPDVPDGPYQTDGISSTVVPFRNGI
                     MLSVAVGYA"
BASE COUNT          394 a          738 c          751 g          425 t
ORIGIN      
        1 gttcagcatc tcggagtaga agacgtcgca ccccccgaaa gagcgcacga gttctcggta
       61 ggccacatgg ctcaggtcca ccatgggggc catgaacagc gaacgcgtct tgaaaggcgg
      121 cataccgtgg tcttaagccg cggggttcct caggacgacg agcgcgtcga ccgccacggg
      181 tatgtccccg tgcacgatca tggggttgat gtcgatctcc gccacctggt ccagctcgag
      241 ccctatcttc ccgaggttga cgagcgtagc cgcgaggacc tccctgttga ccgcgggctt
      301 gcccctgaac tcgtcgagga gctttctggc cttgatctcg tccatcatct cgagggcgtc
      361 cctcgtggta agcggcgcga tgcggaaggt gacgtccttg aggacctcgg tgaagatgcc
      421 gcccagcccg aacatgacgc aggggccgaa ctgggggtcc ctgatgagcc cggccacgaa
      481 ctccctgtcg cccttgacca tctgctgcac caggatcccg tcgagctggc ctgcgccccg
      541 ctcagtgatc gcggcaaagg ccttctccag ggcgtcgtcc ccctggatgc ccagctccac
      601 gaggccgtgc tccgtcttgt gggtgagggt ccgcgaggag cccttgaggg ccaccgggta
      661 ccctatcgcg tgggcggcct cgagcgcctc ctcgaccgtc aacgccacgc gctcctcggt
      721 gacggggata ccgtatgcct tcaggagccg cttcgactcg tattcgttga gcgccttgtc
      781 gccgcgcctg taagcgtctt cgatgatctt catggaagcc ccctcgtctg cttgtatcat
      841 cgtccgtatt cctagcagaa aatgcattcg ctgtgaatgg tcatctgata agccctgaaa
      901 ccatcgaggg ctgcttctct ccattggggg ccccaggcct acagggcgca ccggcgtgca
      961 tgtatcccca aaagacctcc cggatgggct tcccgcacga caggtcacgg ccgagaacat
     1021 gccgacaagg aggcgtcgac cgttttctgc acgagcccct gcggaccacg gggaagccct
     1081 tctcccgggc ggcatggtcc ccgtcgccgg gggcagccca cctcttgccg cctgcggtcg
     1141 gggaggggga agacctcgat cgggcttgca ccgccatctg ccgggagtat aatgatccct
     1201 tggggtttgt cggccggaga cgaggggccg cctgatccca cgcgcagcgg ccctgaccag
     1261 gtcaacgggc cggcaaccgg tgagagcggc ctcccaggag gcgaggagaa gccccgggac
     1321 aggggggaaa gagggctatg aagctcgtgg tcaccgaaat ctttcactcg ctccaggggg
     1381 aaggtccctc catagggacc ccttccgtct tcgttcgtct gggtgggtgc atagagcccc
     1441 tgtgtccctg gtgcgacacg ccgtacgcat ggcacgattt cgaggagatg gatctggagg
     1501 agatcgcgcg cagggtcgcc tcgttcggct gcagggacgt cgtgatcacc ggcggcgagc
     1561 ctttcctcca gtgggacgtg ggtcttgccg atctccacgc atcgctcagg tcctcgggtt
     1621 tccgcatcca ctacgagacg agcggcaagg cggggatccc ctcgatagac gacgcggtcg
     1681 tggtggcgag ccccaagcac atcgacggcg cgtggcacat ccggaggccg gatctcgcga
     1741 acgcgcactg gttcaagttc gtggccgacg gggagcactc cctgagggcc atacggtcgt
     1801 tcgtgtcgga aaacgccata gaccccgaga aggtctatgt catgcccatg ggcgccacgc
     1861 gggcgcaaca gatggaacgg atgcggcccg tgttcctgtt ctgcagggac aacggcctca
     1921 agatgtcgcc gaggctccag gtcctcgtgt tcgacgacga aaggggggtg tgaaggtgga
     1981 agacgcggtc atcctgctct cggggggaat ggactcgggt gtgctcctcg cctgggccaa
     2041 ggaacgctac cgtgagatcc acgcgctcac gttcaactac ggatccaagc acgccggaag
     2101 ggagaccgcc atggcatgcc ggttggccca gcactacggg gtgaccttct ttctcgcgga
     2161 actccccttc gttcgcgagc tcttcagatc gagcctgctg gccggaggcc ctgatgtccc
     2221 ggacgggccg taccaaaccg acgggatatc ctcgacggtc gtgccgtttc gcaacgggat
     2281 catgctctcg gtggcggtcg gctatgct
//
LOCUS       DAOSCR010000459         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30502_length_2308_cov_2.437639, whole genome shotgun sequence.
ACCESSION   DAOSCR010000459 DAOSCR010000000
VERSION     DAOSCR010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30502_length_2308_cov_2.437639"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLT69_10335"
     CDS             complement(<1..318)
                     /locus_tag="PLT69_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14879.1"
                     /translation="MVDKVLAFLMPTLALLSIGVLFLAACKAVSFLGRAGGWTVRVGV
                     DPRRVRGPSVVLFPVCEDLLCCGLAGFVVVNRPTGKAGDPEREISELVEQVCSRGLSA
                     PGAP"
     gene            complement(413..1576)
                     /gene="carA"
                     /locus_tag="PLT69_10340"
     CDS             complement(413..1576)
                     /gene="carA"
                     /locus_tag="PLT69_10340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOJ14880.1"
                     /translation="MKLAGYDTRPGVVVLEDGTVYRGRVFAGEGSRFGDVVFNTGMTG
                     YQEILTDPSYKEQILVMTYPLIGNYGVNSADVESWGIHLEAFVVREYQRRPSNWRMEK
                     TLADYLCEHGTIGIEGVDTRALTRRIRTQGAMRGVVSTEVDRIDDLVDRVRAYPGLVG
                     RDLVRDVTGAGAYVWKDGEKAGMEAARGRSGLPRVVVVDCGVKLNILRLLEERGCEVV
                     VVPAHTSSGAILDLAPQGVLLSNGPGDPAPLGYLVETVRGVLGRVPVFGICLGHQILG
                     QALGGSTLKLKFGHHGINHPVKNLATLRVEISSQNHGFVVDPATLPKDVEITHVNLND
                     MTLEGMRARDLDAFSVQYHPEAAPGPRDSNYLFEEFMASIGCGPRPGTDPHLA"
     gene            complement(1684..>2308)
                     /locus_tag="PLT69_10345"
     CDS             complement(1684..>2308)
                     /locus_tag="PLT69_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOJ14881.1"
                     /translation="GSGMHVHQSLFKGDKNAFLDPKDKYHLSPVGKGYIAGILKHARE
                     IVAVTNQWVNSYKRLVPGYEAPVYVSWARRNRSAMVRVPMYKPGKANATRMEFRAPDP
                     ACNPYLAFAVQIAAGLEGVVKKYPLPEPIEQDIFEMDEHARAQAGITSLPGNLFEAIQ
                     EVSKSALVKEALGDHIFEKFIENKKKEWDNFRIHVSKYEIDTYLPLL"
BASE COUNT          370 a          789 c          722 g          427 t
ORIGIN      
        1 gggggcgccc ggtgccgaga gccctcgaga gcagacctgt tcgaccagct ccgagatctc
       61 ccgctcgggg tcgccggcct tgcctgtggg cctgttcacc acgacgaagc cggccaggcc
      121 gcagcacaag aggtcttcgc acacggggaa gaggacgacg gacggtcccc gcacccttct
      181 cgggtcaaca cccaccctca ccgtccatcc tccggcgcgt ccgaggaagg agaccgcctt
      241 gcatgcggcg aggaaaagga cgccgatgga gagcagggcc agcgtgggca tcaggaaggc
      301 gagaaccttg tctaccatgg gcgcctcacg tgtgggaggt gttcgtggac tgagcgaccg
      361 ggatcactcg tacaccccac ttgagcacgg ctgtatatca ccccgggcgc catcaggcaa
      421 gatgcgggtc tgtccccggc ctcggcccgc acccgatgct cgccatgaac tcctcgaaca
      481 ggtagttcga gtccctcggt ccgggagccg cctcggggtg atactggacg ctgaacgcgt
      541 cgaggtccct ggcgcgcatc ccctcgagcg tcatgtcgtt gaggttcacg tgggtgatct
      601 ccacgtcctt gggcagcgtc gcgggatcga cgacgaaccc gtgattctgg gacgagatct
      661 ccaccctgag cgtcgccagg ttcttcaccg ggtggttgat gccgtggtgc ccgaacttga
      721 gcttgagcgt ggagccgccc agcgcctgtc cgagtatctg gtgcccgagg cagatgccga
      781 acacgggtac cctgccgagt acccccctca cggtctcgac gaggtagccc aacggggccg
      841 ggtcgccggg gccgttcgac aggaggaccc cctggggcgc caggtccagg atcgctcccg
      901 acgaggtgtg ggcagggacc accacgacct cgcacccccg ttcctcgagg agacgcagga
      961 tattgagctt caccccgcag tccaccacca ccacgcgcgg cagccccgat cggcctcgtg
     1021 ccgcttccat ccccgccttc tcgccgtctt tccacacgta ggccccggcg ccggtcacgt
     1081 ccctgaccag gtcccggccg acgagaccgg ggtaggcccg aaccctgtcc acgaggtcgt
     1141 cgatgcgatc gacctccgtg gagacgaccc cgcgcatggc accctgggtg cggatgcgtc
     1201 gcgtgagggc ccgcgtgtcg accccctcga tccctatggt accgtgctcg cacaggtaat
     1261 ccgcgagggt cttctccatg cgccagttgc tcggcctgcg ctggtattcc cgcaccacga
     1321 acgcctcgag atggataccc cacgactcca cgtccgcgga gttgaccccg tagttcccga
     1381 tgaggggata cgtcatgacg aggatctgtt ccttgtagga ggggtcggtg aggatctcct
     1441 ggtatcccgt catcccggtg ttgaagacca cgtcgccgaa gcgcgagccc tcaccggcga
     1501 atacccggcc cctgtagacg gtgccgtctt cgagcaccac cacgccgggc ctggtatcgt
     1561 atcctgcgag cttcatgcac cccttgaatc cgcttggtga acccctgtgg aagatcctca
     1621 cgtcttcgtt cgtcaccctg cggcgcaagg gttatcgccc cgcgccgcag ggtccgggtt
     1681 cttctacagc aaggggagat acgtgtcaat ctcgtacttg ctcacgtgta tgcggaagtt
     1741 gtcccattcc ttcttcttgt tctctatgaa cttctcgaag atatggtctc ccagcgcctc
     1801 cttcacgagc gcactcttcg atacctcctg gatggcctcg aagaggttgc ccgggagcga
     1861 cgtgatgccg gcctgcgcgc gcgcgtgctc gtccatctcg aagatgtcct gctctatcgg
     1921 ctcgggaagc gggtacttct tcaccacgcc ctcgaggccc gcggctatct gcaccgcgaa
     1981 cgccaggtag gggttgcacg ccgggtcggg cgccctgaac tccatgcggg tggcgttcgc
     2041 tttcccgggc ttgtacatgg ggacgcgcac catggccgaa cggttcctcc tcgcccacga
     2101 cacgtacacc ggggcctcgt agccgggcac caggcgcttg taggagttca cccactggtt
     2161 ggtcaccgcc acgatctcgc gggcgtgctt gagaatgccg gcgatgtacc ccttgcccac
     2221 cggggagagg tggtacttgt ccttggggtc gaggaaggcg ttcttgtccc ccttgaacag
     2281 ggactggtgg acgtgcatgc cgctcccg
//
LOCUS       DAOSCR010000460         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30525_length_2306_cov_3.814749, whole genome shotgun sequence.
ACCESSION   DAOSCR010000460 DAOSCR010000000
VERSION     DAOSCR010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30525_length_2306_cov_3.814749"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1523
                     /locus_tag="PLT69_10350"
     CDS             279..1523
                     /locus_tag="PLT69_10350"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ14882.1"
                     /translation="MQKVSVPSRLWYENTEREIEFPDRWQVDVLTSPGLERPALGPEE
                     IRRRIENPVDGPRLSDLARNRRQAVIVFDDMTRPTPVKDVVPFVLEELHRAGMKKEQI
                     RFIWALGSHGAYGMADARKKLGDHVVENYAVYNHDPFQNTVYVGRTPTGVELWVNREF
                     MACDLKIGIGCITAHVHVGFGGGAKIVLPGVAGIESINQFHNQQFRDFGRTGLGNFEN
                     NIMRAECDAAGEMVGLDFKVDCLVNRRGRITSLYAGPFKVTHAMGAEEGKEHYGIPYA
                     KGYDIVVSNAYGKANEAAIALSFPVMLLEPGGKGIGVIICDSPEGQVPHYVFRAIGSG
                     YGGRHYTPRAPGFIPLLMKRLVVLNPQPDKTFLDLVCNEADAVVVKTWPQALERLEEE
                     YPGGAKVAVVVDGTVQYLMRPV"
     gene            1859..2275
                     /locus_tag="PLT69_10355"
     CDS             1859..2275
                     /locus_tag="PLT69_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14883.1"
                     /translation="MKFSLTVHNGVETKNGRPRIVVKDVKVRPDAVAIPASALLLKKE
                     KKQPGRKGKAIHDIVLETAKKLSKKAENGVFSSMDLYKQAVKKYPALNKKSFTTTVIA
                     SAPEHPSWKHYRNGKDHLVYLGKGRYRLRVAEGGQA"
BASE COUNT          474 a          698 c          774 g          360 t
ORIGIN      
        1 cgcaaagggt ttgcgggcca ggtctgcttc atcaccgggc cgagttccac gagcgacatc
       61 caggctgtgc agttcaaggg gatgcacggg ccggccaggc tcgaggtgat ccttctcgat
      121 acgtgcgaac gaaaaccggc gggaggcctt tgagtcgcgg ccgtctcttc ggcctgatgc
      181 cggccgggct cgaagggccc ccaggtgagc gaggcggaaa gccggcctct tgaaggggcc
      241 gggcgtggcc ggggtggtcc ccggggagga gaaagatcat gcaaaaggtc tctgtaccct
      301 cgcgtctgtg gtacgaaaac accgagcggg aaatagagtt ccctgaccgg tggcaggtgg
      361 atgtcctcac gagcccgggg ctcgagcgtc ccgccctggg cccggaggag atccgcagga
      421 ggatcgagaa cccggtggat ggcccgaggc tgtcggatct ggcacgaaac aggcgccagg
      481 cggtcatcgt cttcgacgac atgacgaggc ccacccccgt caaggacgtg gtgcccttcg
      541 tgctcgagga gctccacagg gcgggcatga agaaggagca gatacgtttc atctgggccc
      601 tcggcagcca cggtgcgtac ggcatggccg atgcgaggaa gaagctcggg gaccacgtgg
      661 tcgagaacta cgccgtctac aaccacgacc ccttccagaa cacggtgtac gtggggagga
      721 cccccaccgg cgtcgaactg tgggtgaaca gggagttcat ggcgtgcgac ctcaagatcg
      781 gcatcgggtg catcacggcg cacgtgcacg tgggattcgg tggcggagcc aagatcgtcc
      841 tgccgggcgt ggcgggcatc gagtccataa accagttcca caaccagcag ttcagggact
      901 tcggccgcac cgggctcggg aacttcgaga acaacatcat gcgggcggag tgcgacgcag
      961 ccggcgagat ggtaggcctc gacttcaagg tcgactgtct cgtaaaccgc aggggccgca
     1021 tcacgtcgct ctacgcaggc ccgttcaagg tgacccacgc gatgggtgcc gaagagggca
     1081 aggaacatta cggcataccg tacgcgaagg gctacgacat cgtggtgtcc aacgcctacg
     1141 gcaaggccaa cgaggccgcc atcgccctgt cgttcccggt catgctcctg gagccgggag
     1201 gcaaggggat cggggtcatc atctgcgaca gtcccgaggg gcaggttccc cactacgtgt
     1261 tcagggcgat cggcagcggc tacggcggca ggcactacac gccgagggcc ccgggattca
     1321 tacccctcct catgaagagg ctcgtcgttc tcaacccgca gcctgacaag accttcctcg
     1381 acctggtgtg caacgaggcg gacgccgtcg tggtcaagac gtggccccag gcgctcgagc
     1441 ggctcgagga ggagtacccg ggcggcgcga aggtcgccgt ggtcgtagac ggcaccgtcc
     1501 agtacctcat gaggcccgtg tagggtcccg ggggctttcc cgggctgggg caggcggacg
     1561 gaaggagaac gggcccccgg tcgatcgaga ccgacgggcc tcgtgcatga aggcgtcggc
     1621 cccgccgatt gtcggcccgc acgcgggcgg gaggccttcc tgcgggcgcc tgggcgctcg
     1681 cggagcgtga ccgcccgggc tcacgggaag gtgaaggcgg ctgcgcgcaa cccacgcatg
     1741 gaggccgtca agacggaaac ggccgacttt tccgattatt ccttcttgca agccgcatga
     1801 tttcctttta tacattttgc aattaccatt cagaggggtg atcatgagaa aacgtgctat
     1861 gaaattctct ttgaccgtgc ataacggcgt cgagacaaag aacggcaggc ccaggatcgt
     1921 tgtgaaagat gtcaaggtca ggcccgacgc cgtggccata cctgcctcag ccctcctcct
     1981 gaagaaagag aagaaacaac ccggccggaa agggaaggcc atccacgaca tcgtcctcga
     2041 gaccgcgaaa aagctctcca agaaggcgga aaacggagtc ttctcgtcga tggatctcta
     2101 caagcaggcg gtcaagaaat accccgccct gaacaagaag agcttcacca ccaccgtgat
     2161 cgcgtcagcc cccgagcacc cctcctggaa gcactaccgg aacggcaaag accacctcgt
     2221 ctatctcggc aaggggaggt accgccttcg cgtggccgaa ggcgggcagg cttgaatccc
     2281 gctcactcgg cagagagggg gatggt
//
LOCUS       DAOSCR010000461         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30528_length_2306_cov_3.561084, whole genome shotgun sequence.
ACCESSION   DAOSCR010000461 DAOSCR010000000
VERSION     DAOSCR010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30528_length_2306_cov_3.561084"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLT69_10360"
     CDS             <1..558
                     /locus_tag="PLT69_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOJ14884.1"
                     /translation="NKEAVQRVNKFSNMAVIADVTNKETLVGLGIADCDAAVVSLGDH
                     PAASTLVTLYLHEMGVKQILVKAIDENHAKILRKVGATDIIFPEKDMGIKVAKTLVSP
                     NILDFIEMTEDYEITQIGPPASFIGKTLMELELPSRFGLQVIAVRELIPENFIPAPRA
                     DFVIKDSDILYILGRKKDIERIKAL"
     gene            891..2300
                     /locus_tag="PLT69_10365"
     CDS             891..2300
                     /locus_tag="PLT69_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ14885.1"
                     /translation="MMITRLLGIAQAAFVISSLALGLQARAAGAADQGTELRYAVTKV
                     AKALLPSVVHIEISQERAKDPTAAQGLFQESAPAEEQETALKFKQEVTALASGIILDD
                     KGYIITNNHVVEGASSIEVTLWSGRRYPARLEGADPKTDIAVIKIEPDTTLSPAVFGD
                     SDRVEIGEWVVAIGNPRGLDLTVTQGIISAKDRRRILDPSSYLSFLQTDAAINPGNSG
                     GPLINLEGKVIGINSVIASESGGFEGIGFAIPGNIALSTARSLIAHGRVKRGWIGASV
                     DDLDEAKARELDLPSPKGVLVIDVIKDGPADKAGIKTGDVITAAAGRELLDAAMFRNI
                     IALFEPETDLPLEILRAGSSESRKVRLVDLESSLRLIASTVKARMGVEARSLKQQEKE
                     TFGIETGVAVSWLDPAGPLAKAGFEAGDIILEMDGQMIRNVHDFASIAATILPRQVTI
                     LLAIDHRSGESAYVQVKAR"
BASE COUNT          480 a          739 c          733 g          354 t
ORIGIN      
        1 aacaaggagg cggtgcagcg ggtcaacaag ttctcgaaca tggcggtgat agcggacgtc
       61 acgaacaagg agacgctcgt gggcctcggc atagcggact gcgacgcggc ggtcgtgagc
      121 ctgggggatc acccggccgc atccaccctg gtgacgctct acctccacga gatgggcgtg
      181 aagcagatcc tcgtcaaggc catcgacgag aaccacgcga agatcctcag gaaggtgggg
      241 gccaccgaca tcatcttccc ggagaaggac atgggcatca aggtggcgaa gaccctcgtc
      301 agcccgaaca tcctggattt catcgagatg accgaggact acgagataac gcagatcggc
      361 ccgcctgctt cgttcatagg caagaccctc atggagctcg agctgccgag caggttcggg
      421 ctccaggtga tagcggtgcg cgagctcatc ccggagaact tcatccccgc gccgagggcc
      481 gacttcgtga tcaaggacag cgacatcctc tatatcctcg gcaggaagaa ggacatcgag
      541 cgcataaagg cgttgtgacc cccgggagta gggcgaaggg aaccccggcg gtcggtctcg
      601 gcgcccgttg ggaaaacccc gggaaagggt gtcccgcccc atggatacag cctctgcgct
      661 tcgtcttgcg tccccctcgc actgaagaga ccgcccggtg gacgcccttg ctcgaaggcg
      721 cctccccggg cgctgcaaac gcccgcaccc ggattacgaa gacgaccggg ctcggtggcc
      781 gtatcccctg ttcaccctgg cgtaacaccc ttggatcacc cttgtattcc tcacaggcag
      841 tgatatcatg gagacgagca ctcgaggcgg aggttgcgga gaaaaccccc atgatgatca
      901 cacgtcttct cggcatcgca caggcggcgt tcgtgatttc gtccctggcc ttaggcctcc
      961 aggcccgcgc ggcaggcgcc gccgaccagg gtacggaact caggtacgcg gtgaccaagg
     1021 tcgccaaggc cctcttgccc tcggtggtcc acatcgagat ctcccaggaa agggccaagg
     1081 acccgacggc cgcccagggg ctcttccagg agtctgcgcc ggccgaggag caggagaccg
     1141 cgctcaagtt caaacaagaa gtgaccgcgc tcgcgagcgg catcatcctg gacgacaagg
     1201 ggtacatcat cacgaacaac cacgtggtcg aaggtgcatc ctccatcgag gtgaccctct
     1261 ggtccggccg gaggtaccca gcccgtctcg aaggggccga cccgaagacc gatatcgccg
     1321 tgatcaagat cgaacccgac acgacgctct ctccggccgt gttcggagac tcggacaggg
     1381 tcgagatcgg cgagtgggtc gtcgccatag gcaacccgcg cggcctcgac ctgacggtca
     1441 cccagggcat catcagcgcg aaggacagga gacgcatctt ggacccgtcc tcgtaccttt
     1501 ccttcctcca gacagacgcg gccatcaacc cgggcaacag cggggggccc ctcatcaacc
     1561 tcgaagggaa ggtcatcggc atcaactcgg tcatcgcatc cgagtcgggg ggcttcgagg
     1621 gtatcgggtt cgccataccc ggcaacatcg cactgtcgac ggccaggtca ctgatagcgc
     1681 acggcagggt caagcgaggg tggataggcg cctccgtgga cgacctggac gaggcgaagg
     1741 ccagggaact ggaccttccc tcccccaagg gggtcctcgt gatcgacgtg atcaaggacg
     1801 ggcctgccga caaggcaggg atcaagacgg gcgacgtcat cacggcggct gccggcaggg
     1861 agctcctgga cgcggccatg ttcaggaaca tcatcgccct cttcgagccg gagacggacc
     1921 tgcccctgga gatactcagg gcaggttcgt cggaaagcag aaaggtcagg cttgtcgacc
     1981 tcgagtcctc gctcaggctc atcgcctcca ccgtcaaggc ccgcatggga gtggaagcga
     2041 gaagcctcaa gcagcaggag aaggaaacct tcggcatcga gacaggtgtc gcggtctcct
     2101 ggctcgaccc tgcaggcccc ctggccaagg cggggttcga ggcgggcgac atcatcctcg
     2161 agatggacgg ccagatgatc aggaacgtgc acgacttcgc atcgatcgcg gccacgatcc
     2221 ttccccggca ggtgacgatc ctgctcgcca tcgaccatcg aagcggcgag agcgcctacg
     2281 tccaggtgaa ggcccgctga aggctc
//
LOCUS       DAOSCR010000462         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30547_length_2305_cov_3.252444, whole genome shotgun sequence.
ACCESSION   DAOSCR010000462 DAOSCR010000000
VERSION     DAOSCR010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30547_length_2305_cov_3.252444"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /gene="miaB"
                     /locus_tag="PLT69_10370"
     CDS             <1..1117
                     /gene="miaB"
                     /locus_tag="PLT69_10370"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ14886.1"
                     /translation="RIPSAVIVVTGCVAQQEQGRILERMGHVDIVLGTHQLHLLEALV
                     EEARAGRGPVVAAGFSPCVPFMDTVFDHGLSPEPHRAYVTVMQGCDNYCSYCIVPYVR
                     GPEISRDASKVLEEIRLHAGRGSREVFLLGQNVNSYRGGMSFPELLRRIHDIEGIDRI
                     RFTTSHPKDAGDDLVACFKDLPKLCSHIHLPFQSGSNRVLAAMNRKYTKETYLELVSK
                     FRQARPDMAFSADVIVGFPGEGVNDFDETMDVVERVRFDVLFSFKYSVRPNTKAASLV
                     DDVHGEEKSRRLDILQRRQREITLENNRLRVGGVYDVLVDGASKKHEHQVHGRTTHNV
                     IVNFDGPRELTGSIVKVRITRANPNSLTGELLASPPS"
     gene            1464..>2305
                     /locus_tag="PLT69_10375"
     CDS             1464..>2305
                     /locus_tag="PLT69_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ14887.1"
                     /translation="MKEPKRWPTIAVLVAAQAAWIVIVAVWVVWYVKYRQIKMEPFGM
                     WDVVILAEVSGLFTLILAGIYVLFILYQRQLSLMRTQMYIVQSVTHAFKTPIATMQLY
                     LETMASRDLPEKTKAELIEGMLAVNARLKSLVYNFLESARLSSERRHYAFSEKDLAGL
                     VTSCIENHRVFLKGVDLELKAEKDVTVRVDQDAFDLVFSNLVENAVHYSDGQARVEIE
                     ISKDDRWAYLTFSDSGIGIPRERHKDIFKMFRRLPEAVSMWGSGTGMGLFVVRGIVEA
                     HGGS"
BASE COUNT          462 a          726 c          711 g          406 t
ORIGIN      
        1 caggataccg tccgcggtca tcgttgtcac cggctgcgtg gcgcagcagg aacagggccg
       61 catcctggaa cgcatgggac acgtggacat cgtgctcggc acccaccagc tccacctcct
      121 cgaggccctc gtggaggagg caagggccgg gagggggcct gtggtggccg cgggattctc
      181 gccctgtgtc ccgttcatgg acacggtgtt cgaccacggg ctctcccccg aaccccaccg
      241 ggcgtacgtg accgtgatgc aggggtgcga caactattgc tcgtactgca tcgtgccgta
      301 tgtgagaggt ccggagatca gcagggatgc atcgaaggtc ctcgaagaga tcaggctcca
      361 cgccgggcgc gggtcgaggg aggtcttcct gctcggccag aacgtgaact cctaccgagg
      421 cggcatgtcg ttcccggaac tcctcaggag gatccacgac atcgagggga ttgaccgcat
      481 ccgcttcacc acctcccacc ccaaggacgc gggcgacgat ctcgtcgcgt gcttcaagga
      541 tctcccgaaa ctgtgcagcc acatacacct ccccttccag tccggttcga accgggtgct
      601 tgccgccatg aacaggaagt acacgaagga gacctacctc gaactcgtct cgaagttcag
      661 gcaggcgcgc cccgacatgg cgttcagcgc ggacgtgatc gtcggctttc cgggagaggg
      721 cgtgaacgac ttcgatgaga ccatggatgt cgtcgagagg gtgaggttcg acgtcctctt
      781 ctcgttcaag tactctgtca ggccgaacac aaaggctgca tccctggtgg acgacgtcca
      841 cggcgaggag aagtcccgga ggctcgatat cctccagagg cgccagaggg agataacgct
      901 agagaacaac aggctccggg tgggcggagt atacgacgtc ctcgtggacg gcgcctcgaa
      961 gaaacacgaa caccaggtcc acggccgcac cacccacaac gtcatcgtga acttcgatgg
     1021 gccgcgagag ctcaccggat cgatcgtgaa ggtgaggata acgcgcgcca accccaactc
     1081 gctcaccggc gagttgctcg cctccccacc ctcatgatga gaccgtgcac acgcccctga
     1141 aggccatgga taggggcgtg tccacaaccg ttcagggcgg gatccggcct gccgagaccc
     1201 cctggccagc ccacgaatgc ccacgtcagc cggctgtgaa gcgagagaga tgggagcgca
     1261 ccggttcctg tcccggttca ccggagaacc tgcgtccgcc gggaaatccc cttgtgtgcc
     1321 catggggcct cccggccttc ggtcctcccg gcctccaggc ccagaaggct accgcggctt
     1381 atgcactgtt tgctcaagga acgcttcccg gcgcatccga aagctgtgga ctcgcacttt
     1441 ccgcaatgat atgggaaggg tacatgaaag aaccgaaacg ctggcccacc atagcggtcc
     1501 tcgttgcggc ccaggccgca tggatcgtca tcgttgccgt atgggtcgtc tggtacgtca
     1561 agtaccgcca gatcaagatg gaacccttcg gcatgtggga tgtggtcatc ctggccgagg
     1621 tctcgggcct cttcaccctc atcctggcgg gcatctacgt gctcttcatc ctctaccaga
     1681 ggcagctttc cctcatgagg acccagatgt acatcgtcca gtccgtgacg cacgcgttca
     1741 agacccccat agccaccatg cagctctacc tggagaccat ggcatcaagg gatttgccgg
     1801 agaaaacgaa agccgagctc atcgagggca tgctcgcggt gaacgcaagg ctcaagtcgc
     1861 tcgtgtacaa cttcctcgag agcgcgaggt tgagctccga gcgaaggcac tacgccttct
     1921 cagagaagga tctcgcgggc ctcgtgacct catgcatcga gaaccacagg gtcttcctca
     1981 agggggtcga tctcgagctc aaggcggaga aggacgtaac ggttcgtgtg gaccaggacg
     2041 ccttcgacct ggtattctcg aacctcgtgg agaacgcggt gcactactcg gatggtcagg
     2101 cgcgcgtcga gatcgagatc tccaaggacg acaggtgggc ctacctcacc ttctccgact
     2161 cgggcatagg cattcccagg gagaggcaca aggacatctt caagatgttc cggcgcctgc
     2221 ccgaggccgt cagcatgtgg ggctcgggca ccggcatggg cctgttcgtg gtcaggggca
     2281 tcgtcgaggc ccacggggga tcgat
//
LOCUS       DAOSCR010000463         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30649_length_2300_cov_4.122049, whole genome shotgun sequence.
ACCESSION   DAOSCR010000463 DAOSCR010000000
VERSION     DAOSCR010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30649_length_2300_cov_4.122049"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLT69_10380"
     CDS             <1..762
                     /locus_tag="PLT69_10380"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ14888.1"
                     /translation="LYPTQELARHAGMSIEEYTGQIVDACRLDVDDPVAWWKETYGQA
                     QEIKRWLNGMKIKRLFVQSDTTDLVVPPGASRKWVGISGHNIPSFEIFVSPDWRGTSG
                     RFYADQPSYRSGNYVKGVELEFDRGVVVKARARDGEEFLLKQIAMDRGASRLGEFSLT
                     DRRFSRINAFMANTLYDENYGGEHGNCHIALGDSYADTYDGDASLLSPQKKRSLGFND
                     SALHWDLVNTERKRVTAELGDGKRVTIYEDGVFSC"
     gene            1116..>2300
                     /locus_tag="PLT69_10385"
     CDS             1116..>2300
                     /locus_tag="PLT69_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14889.1"
                     /translation="MSGKDVREELRNRVHRLEDRLRRLKRLAEGLVDAAGRYRIHFSL
                     ANDVIYSIDRDFIVTSVSPSVERVLGYRPEELVGRPFYEHRVIPEEYVQKAFDEIRRV
                     LGGEKIVSSVYTFITKDGRVKYGEVTGVPYQKGGRPVEVISVARDITERIEMERSLRM
                     NEQLFRAVFESARDCIFLKDTSLRYTYVNPCMERALKVSSRDIVGRTDGDVFGPRAAA
                     LSEPMDKRVLAGEELEHECVWAPAGEEIVMHVVKVPIRDLSTQTITGLCGIARDVTER
                     RRAEERLKSKEHELELQACRLEEMNVALRVLLDAREREKREAIERLAEGVRKRVVPYL
                     ERLRGGLNEGSRTYLDAAQANIEDLFGSNMGGLERLSFRLTPMELRVAELVRLGKSTK
                     EIA"
BASE COUNT          462 a          688 c          781 g          369 t
ORIGIN      
        1 ctctatccaa cccaggagct cgcacgccac gcgggcatga gtatcgagga atacaccggc
       61 cagatcgtgg atgcatgccg cttggacgtc gacgaccccg tcgcgtggtg gaaggagacg
      121 tacggccagg cccaggagat caagcgctgg ctcaacggga tgaagatcaa gaggctcttc
      181 gtccagtcag acacgacgga cctcgtcgtc ccccccggcg cgagcaggaa gtgggtgggg
      241 atatcgggac acaacatacc gagcttcgag atcttcgtgt cgccggactg gcggggcacc
      301 tccggcaggt tctacgccga ccagccctcg tacaggagcg gcaactacgt caagggggtc
      361 gaactcgagt tcgaccgcgg cgtcgtggtg aaggcccgcg cccgggatgg ggaggagttc
      421 ctcctgaagc agatcgccat ggacaggggg gcctcgaggc tgggggagtt ctccctcacc
      481 gacaggaggt tctcgaggat aaacgctttc atggcgaaca ccctgtacga cgagaactac
      541 ggcggcgagc acgggaactg ccacatagcc ctgggagact cgtacgccga cacgtacgac
      601 ggggatgcct ccctcctcag cccgcagaag aagaggtcgc tcggtttcaa cgattcagcc
      661 ctccactggg acctcgtgaa cacggaacgg aagcgggtca cggccgagct cggggacgga
      721 aagagggtga cgatctacga ggacggcgtc ttttcctgct gagaccctcc acctcgcgcc
      781 gcctgcgtcc ctgtgtcggg ggccttcccg tgagcctcca ctccaggggg atcacggcct
      841 tttcagggcg tcggggatct cggccggggg ggtccggcga gacccgcgta ccgcctctca
      901 agggatcgcg tccccctcgg gactccggtg atctcggcag aaccgcctcg ttgtcgagcg
      961 ccctcgaccg aggtctatca cccgtgggcg cccgtgattt caacatcgta attgccatgt
     1021 ggatgtcgat gcggtaacat gcccgagtgc cggggcttac cggtgcatgc acgtctttgt
     1081 gatgaatgtg tagtgatgca atgagggcag gtcggatgag cggcaaagat gtacgcgaag
     1141 agctgaggaa cagggtacac cgtctcgagg acaggctcag gaggctcaag cgcctcgccg
     1201 aaggcctcgt ggacgcggcc gggcgctacc ggatccattt cagccttgcc aacgacgtca
     1261 tctattccat cgacagggac ttcatcgtca cgagcgtgtc cccgagcgtc gagcgcgtgc
     1321 tcggctacag gcccgaagag ctcgtgggca ggcccttcta cgagcaccgc gtcatccccg
     1381 aagagtacgt ccagaaggcc ttcgacgaga tacggcgcgt gctcgggggg gagaagatcg
     1441 tgtcgtccgt ctacaccttc atcaccaagg acggacgggt caagtacggg gaggtgaccg
     1501 gcgtccctta ccagaaaggc ggcaggcccg tggaggtcat ctccgtggcc cgcgacatca
     1561 ccgagcgcat cgagatggag cgcagcctcc ggatgaacga acagctcttc agggcggtgt
     1621 tcgaatcggc ccgggactgt atcttcctca aggacacgtc actgaggtac acgtacgtga
     1681 acccgtgcat ggagcgggcc ctcaaggtaa gctccaggga catcgtcggc aggacggacg
     1741 gggatgtctt cggcccgagg gcggcagccc tctcggagcc gatggacaag agggtccttg
     1801 ccggcgagga gctcgagcat gagtgcgtat gggcgcctgc cggcgaggag atcgtcatgc
     1861 acgtcgtcaa ggtgccgata cgggatctct cgacccaaac cataacgggg ctctgcggta
     1921 tcgcaaggga cgtgacggaa cgcaggcggg cggaggagag gctgaagagc aaggaacacg
     1981 aactcgagct gcaggcctgc cggctcgagg agatgaacgt ggccctcagg gtccttctcg
     2041 acgcccggga gagagagaag agggaggcca tcgagcgcct cgccgaaggg gtgcgcaagc
     2101 gggtcgtccc ctacctggag aggctcaggg gcggcctcaa tgaaggctcg aggacctacc
     2161 tggacgccgc gcaggcaaac atcgaggatc tcttcggctc caacatgggc gggctcgaac
     2221 gtctctcttt ccgtctcacg cccatggaac tgagggtggc ggagctcgtg agactcggca
     2281 agagcaccaa ggagatagcg
//
LOCUS       DAOSCR010000464         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30670_length_2299_cov_3.630125, whole genome shotgun sequence.
ACCESSION   DAOSCR010000464 DAOSCR010000000
VERSION     DAOSCR010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30670_length_2299_cov_3.630125"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PLT69_10390"
     CDS             complement(<1..1417)
                     /locus_tag="PLT69_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ14890.1"
                     /translation="MKPVGVSGNLARAFITSKLTPLSIVGSLILGVFAVLDTPREEEP
                     QIVVPMIDVMVSYPGASPEEVESRVTIPMERLLWEIPGVEYLYSIVGPGSNLTIVRFY
                     VGEDMEDSLVKLTTKLSANYDIIPPGVSQPLIKAKSIDDVPVLALTLWCENGRYTGYD
                     LRRMALELACEIKKGRDVSECTVAGGQRRRLKVELDPGRLRAYRTTAAEIAEAIERSN
                     VTVPAGYLFTGDKVTDVQAGAFLKDASAVERLPVRAHGGRVVYLGDVAEVKDGPEDPR
                     EYVLMGMGPAFDPKGSGKPGRGLFEAVTVAVSKKKGTNASTMVNDVLAKVEALEGVIL
                     PGDVKIAVTRNYGETAQEKSDELLLHMFVATASVIVLIAVALGMREAFVVAVAVPVTL
                     ALTLLVNHLLGYTLNRVTLFALIFSIGILVDDAIVVVENISRHFRARKADPSTAVLAV
                     DEVGNPTILATFTVIAALMPMA"
     gene            complement(1414..1944)
                     /locus_tag="PLT69_10395"
     CDS             complement(1414..1944)
                     /locus_tag="PLT69_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ14891.1"
                     /translation="MTVKRVETGDMAVPGTPLLEMESIEGAYIEADVDQGLGGVVRPG
                     MPVVVRLDAPARTLKGSIRDVLPDVDPVTRTFRIKIDVPHTPLRSGLSCRVEIQTGQR
                     QEVVVPKQAVVRRGHLSAVYVVGDSNLVTLRLIREGPLRDSGVVVLSGLAPGERIVTQ
                     GVEKAFDGGIAKESDR"
     gene            complement(1941..>2299)
                     /locus_tag="PLT69_10400"
     CDS             complement(1941..>2299)
                     /locus_tag="PLT69_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14892.1"
                     /translation="AAQAVAQRHVRRERSELGAAHPLAPAADVGIDPGDPIGLGQAAL
                     EALDAASQRLSLASSTWKRMKALYERQAVSLQSLDEAEISMKVARAEYERALASVREA
                     RAYLDYTRITAPFAGV"
BASE COUNT          367 a          777 c          745 g          410 t
ORIGIN      
        1 aggccatggg catgagggcg gcgatcaccg tgaaggtggc gaggatcgtg gggttgccca
       61 cctcgtccac ggcgagcacg gccgtggagg ggtccgcctt gcgtgcccgg aagtggcggc
      121 tgatgttctc gaccaccacg atggcgtcgt cgacgaggat gccgatggag aagatgaggg
      181 cgaacagggt cacccggttc agggtgtacc cgagaagatg gttcacgagg agcgtcaagg
      241 ccagggtcac cggcacggcc actgccacca cgaacgcctc cctcatgccc agcgcgacgg
      301 cgatgaggac gatcaccgac gcggtcgcca cgaacatgtg caggaggagc tcgtcggatt
      361 tctcctgcgc cgtctccccg tagttcctgg tcaccgctat cttgacgtcg cccggcagga
      421 tcactccctc gagggcctcg accttcgcga gcacgtcgtt caccatggtc gaggcattcg
      481 tccccttctt cttcgagacc gccacggtga cggcctcgaa cagcccccgg cccggcttgc
      541 ccgagccctt cgggtcgaag gccggaccca tccccatgag cacgtactcc ctgggatcct
      601 cgggaccgtc tttgacctcg gccacgtccc ccaggtatac gaccctccca ccgtgggccc
      661 tgactgggag gcgctcgact gcggatgcgt ctttcaggaa cgcgccggcc tggacatccg
      721 tcaccttgtc acccgtgaac aggtatcctg caggcacggt gacattcgac ctctcgatgg
      781 cctcggctat ctcggcggcc gtcgtccggt aagccctgag cctccccggg tccagctcca
      841 ccttcagcct gcgtctctgg ccgcccgcga cagtgcactc cgagacgtcc ctgcctttct
      901 tgatctcgca ggcgagctcg agcgccatgc ggcgaaggtc atagcccgtg tacctgccgt
      961 tctcgcacca cagggtcagg gcgagcacgg gcacgtcatc gatggacttg gccttgatga
     1021 ggggttggga cacgcccggg gggatgatgt cgtagttcgc ggacaacttc gtcgtgagct
     1081 tgacgaggct gtcttccatg tcctcgccca cgtagaaccg cacgatggtg aggttcgagc
     1141 cggggcccac gatggaatac aggtactcca ccccggggat ttcccagagc agcctctcca
     1201 tggggatggt cacgcgcgac tcgacctcct cggggctggc ccccgggtag ctcaccatga
     1261 cgtcgatcat gggcaccacg atctggggct cctcttccct cggtgtgtcc aggacggcga
     1321 acacgcccag gatcagggat cccacgatgg aaagaggggt gagtttcgag gttatgaagg
     1381 cccttgcgag gtttcccgag acgccgacgg gtttcatctg tcggattcct ttgcgatgcc
     1441 cccgtcgaag gccttttcga cgccctgcgt gacgatgcgt tctcctggcg ccaggcctga
     1501 gagcaccacg acgcccgaat cccgtagggg accctccctg atgaggcgca gtgtcacgag
     1561 gttggaatcg ccgaccacgt acaccgcaga gaggtggccc ctgcggacga cggcctgctt
     1621 cgggacgacg acctcctgcc tctggcccgt ctggatctcg acccggcagg acagccccga
     1681 tctcaacggg gtgtgaggga cgtcgatctt tatcctgaag gtgcgcgtga ccgggtccac
     1741 atcgggcagg acgtcccgta tggagccctt cagcgtcctt gcaggggcgt ccaggcggac
     1801 gaccacgggc atccccggtc tgacgacgcc cccgagcccc tggtccacgt cggcctcgat
     1861 gtacgcaccc tcgatgcttt ccatctccaa gagcggggtt ccgggcacgg ccatgtcgcc
     1921 cgtctctaca cgcttgacgg tcacaccccg gcaaaggggg ccgttatgcg cgtgtagtcg
     1981 agataggccc gggcctcgcg cacggaggcg agcgccctct cgtactccgc ccttgccacc
     2041 ttcatggaga tctcggcctc gtcgagggac tggagggaca cggcctgcct ctcgtagagg
     2101 gccttcatgc gtttccacgt cgaggaggcc agggacagcc gctgggatgc ggcgtcgagt
     2161 gcctcgaggg cggcctggcc gaggccgatc gggtcgcccg gatcgatgcc tacgtccgcg
     2221 gctggcgcaa gcggatgcgc ggccccgagc tcggaccgtt cgcgccggac gtgcctctgg
     2281 gcgacggctt gcgcagcat
//
LOCUS       DAOSCR010000465         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30689_length_2298_cov_2.872938, whole genome shotgun sequence.
ACCESSION   DAOSCR010000465 DAOSCR010000000
VERSION     DAOSCR010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30689_length_2298_cov_2.872938"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLT69_10405"
     CDS             complement(<1..195)
                     /locus_tag="PLT69_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ14893.1"
                     /translation="MSSFWEITMGGLIRQTASRYPDNDALIYPETGLRLTYRELLAKC
                     TETAKGFMALGVKNGDHVSVW"
     gene            866..1531
                     /locus_tag="PLT69_10410"
     CDS             866..1531
                     /locus_tag="PLT69_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine nucleotide alpha hydrolase"
                     /protein_id="HOJ14894.1"
                     /translation="MGGKRTLLAWSSGKDSAWALHLLRSDPTHEVVGLFTTVNERVCR
                     ASMHATSLEMLRLQALRAGLPLHTVSLPDPCAAGQYDGVMQRFVEGCRDSGVECMAFG
                     DIFLEDVRAYREQRLKGSGIEPVFPLWGLSTSDLARTMIESGLQAYISCVDLEVLDAG
                     YAGKPWTMDLVEALPKGCDPCGENGEYHTVVFSGPMFSRPIPARVGDIVIRDRFAYAD
                     VMI"
     gene            1765..>2298
                     /locus_tag="PLT69_10415"
     CDS             1765..>2298
                     /locus_tag="PLT69_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14895.1"
                     /translation="MVQKIFLAGLFLAVIFAVAAAFGAYRWEAKTRQIRLRLEEAREP
                     ASRRKVDFRELEGLPTPVQRYFRAVLKDGQPMVEGVHARHAGSFNMGEDSEKWRPFAS
                     DQRVVTARAGIDWDARVSMLPGLRVHVHDAYVAGEGILQAALLGLVPLVDVRGGGEVA
                     RGELMRFFAEAAWYPTAL"
BASE COUNT          369 a          701 c          813 g          415 t
ORIGIN      
        1 ccacaccgag acgtggtcgc cgttcttcac gcccagggcc atgaagccct tggccgtctc
       61 ggtgcacttg gcgagcagct cccggtaggt gaggcggagc ccagtctcgg ggtatatgag
      121 ggcgtcgttg tcagggtatc tcgacgccgt ctgcctgatg agtcctccca tggtgatttc
      181 ccagaacgag ctcatgcgag accctccttg cggtcggttc ttgggtgtaa gccttccttg
      241 tcaaatcaag cagttgaatg atatttcttg acacatgtat cagtctacca ccggctgata
      301 catgtgtcaa tatctatatt cggggcggcg gccgggcacc agtttcttgc ccatgcactc
      361 tctccgggat cggcaggcct cctggcggga ggcgcctaac gcattgggct tccccagggt
      421 ggggccggcc tgtcggggcc cgagaagccg gcaaggacgg ttctgccggg tgctcgggct
      481 cctgcggcga gggccggggg ctttcttggc gggtcatcga agacaggggg gcatgctcgt
      541 ccgccagacc cgtagctata ggcgctgcct gggccggttt tccaggcatg tccagggtgc
      601 cgggaccgaa agcacgctcg tgtcaggcct ctaaggggcg caccatggca aaggcgtacc
      661 gacggcgtca gcctgccatg ccagctggag gcaggtcagt gttggcatgc tgttgacccc
      721 cggcgcacgc tcgggctacc ctcgaggtga cacggccacc gcggcctgcc gccttgatgg
      781 aacgctcaag gagcgggtgg gcatggggcg gtgggcggac gggaggcaaa aggaacggga
      841 aggtcgaatg aggggggaga ggacgatggg cggaaagcgg accctgcttg cctggagcag
      901 cggcaaggac agcgcctggg cgctccacct gctgaggtcg gaccccacgc acgaggtcgt
      961 gggcctgttc accacggtca acgaacgcgt ctgtcgggcc tccatgcacg cgacctccct
     1021 cgagatgctc cgtctccagg cgcttcgcgc agggttgccc ctgcatacgg tctcgcttcc
     1081 cgatccctgc gccgccgggc aatacgacgg ggtcatgcag cgcttcgtgg aagggtgcag
     1141 agacagcggg gtcgagtgca tggccttcgg cgacatcttc ctggaggacg tgcgggcgta
     1201 tcgagaacag aggttgaagg gctccggtat cgagcctgtc ttccccttgt ggggactgtc
     1261 tacgagcgac ctcgcccgga ccatgatcga gtccggcctc caggcctaca tcagctgcgt
     1321 ggacctcgag gtcttggatg cagggtatgc cgggaaacca tggacgatgg acctcgtgga
     1381 ggctcttccc aaggggtgcg acccctgcgg cgagaacggc gagtaccaca cggtggtgtt
     1441 ctcgggcccc atgttctcgc ggcccattcc cgcgcgcgtc ggggacatcg tgatacggga
     1501 ccggttcgcc tacgcggacg tgatgatctg acacctcggt gaacgggaca gcaccgcaca
     1561 ggccagacac gccctgcctg catcccggtg caggcggaga aggcccccct tgtggccttg
     1621 gcgcgcggga accgagcttt tccccctgca cgactcatgg tagtgtggac aatgacgaga
     1681 cggcaggccc acaacgccgt ttgtcggccg ccggttcgtc gatgcctccc gcacgcaagc
     1741 gtggtgagat gggggatgcc gtccatggtg cagaagatct ttcttgcagg actcttcctg
     1801 gccgtgatct tcgcggttgc ggcggccttc ggcgcgtacc gctgggaggc gaagacacgg
     1861 cagatccgct tgcggctcga ggaggcgcga gagccggcca gtcggcggaa ggtcgatttt
     1921 cgtgagctcg agggtttgcc cacgccggta cagcgctact tccgtgccgt cctgaaagac
     1981 gggcagccga tggtcgaagg ggtgcacgcc cggcacgcag ggtctttcaa catgggcgag
     2041 gacagcgaga agtggaggcc cttcgcatcc gaccagcggg tcgtcacggc gcgtgcgggc
     2101 atcgactggg acgcccgggt ctcgatgctg ccggggctcc gtgtccacgt gcacgacgcc
     2161 tacgtggcgg gcgagggcat cctgcaggcg gccctgctcg gtctggtccc tctcgtggac
     2221 gtgcggggcg ggggcgaggt ggcaagaggc gagctcatgc gtttcttcgc agaggcggcc
     2281 tggtacccca cggcgctg
//
LOCUS       DAOSCR010000466         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30796_length_2292_cov_3.534645, whole genome shotgun sequence.
ACCESSION   DAOSCR010000466 DAOSCR010000000
VERSION     DAOSCR010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30796_length_2292_cov_3.534645"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLT69_10420"
     CDS             complement(<1..481)
                     /locus_tag="PLT69_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ14896.1"
                     /translation="MLIFAADIHLRPEAEAETSLFERWASRMLAEGSRLYILGDLFDY
                     WYSGLERRFTRLLEVLATERVLILRGNRDFLLGNRHLAGLHVAEREEVVIEAGSRRIL
                     ACHGHTLTVADSGFRLLHRYGWPALSLLDRALPYALKEPVARLLVSSSRVVRASSLDV
                     "
     gene            734..1480
                     /locus_tag="PLT69_10425"
     CDS             734..1480
                     /locus_tag="PLT69_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOJ14897.1"
                     /translation="MAGYVVVTIVVLVVLFLLSAVKVLNEYERAVVFRLGRVLGTPKG
                     PGLIIIIPLVDRFIRLSMRTIVQDVPPQDVITRDNVTVKVNAVVYFRVIDPLKAVIEV
                     ENYLYATSQLSQTTLRSVCGQAELDELLSKREEVSLHIQSILDRQTDPWGIKVSAVEL
                     KHIDLPEEMQRAMARQAEAERERRAKVINAEGEYQAAQRLFEAAQVIAKEPIAVQLRY
                     LQTVREISSEGSTNTIIPLPIELLGPFLKK"
     gene            1501..2244
                     /locus_tag="PLT69_10430"
     CDS             1501..2244
                     /locus_tag="PLT69_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ14898.1"
                     /translation="MAVLTSRGNKVYKAICRLKRSRDVMVLEGRRLVQDALSRGLRPR
                     TIAVSASFLGAHALPASPDAVFSDALFATLAETETSQGLLALFDVPWARMEEVLGQDR
                     IVVLDGLQDPGNVGTVIRTAEAFGFRGVVILPNTASPFSPKAVRASMGSCLGVAVARG
                     THEEIVASSHRLVALTIDGDETPAPALFEGRFALCLGREASGLSPVLASRAHHRVRIP
                     MAGPTESLNVAVAAGIVMAVAAGVVAGPG"
BASE COUNT          400 a          741 c          796 g          355 t
ORIGIN      
        1 gcacgtccag ggaagaggcc cgcactaccc tggacgagga gaccagcagc cgcgcgacag
       61 gctccttgag ggcatacggc aaggccctgt cgagcaggga caaagccggc cacccgtagc
      121 ggtgaagcag cctgaacccg ctgtccgcga cggtcagcgt gtggccgtgg cacgcgagga
      181 tgcgcctgga gccggcctcg atgacgacct cctctctttc tgcaacgtgt agcccggcca
      241 gatgcctgtt cccgaggagg aagtcgcggt tgcccctcag gatgaggacc cgctcggtgg
      301 ccagcacctc gagcagcctc gtaaaccgcc tctccagccc gctgtaccag tagtcgaaga
      361 ggtctcccag gatgtagagc cgcgagcctt ccgccagcat gcgcgacgcc cagcgctcga
      421 agagggatgt ctctgcctcg gcctcgggtc tcaagtggat gtcggctgcg aagatgagca
      481 ctgatccact aaagacaaaa agcgcccggg aaagcaagcg catcgtcgaa ggcgacgagg
      541 gtcgcggtgc gtgtcgtgcg gggctccggc cgggtgcgtg cccgacgagg gcggggccct
      601 gaggaagcgt gtgcacgacc cggtcaggga gacgggaaac cggccggggg tcccctgcac
      661 aatccccttg acagtcgtcg cgggctggga ccatcgtaca cctcgaggtg caagagactc
      721 ttggaggtga tccatggcag gctacgtcgt cgtcaccatc gttgtcctcg ttgtcctgtt
      781 cctgctctcg gcggtgaagg tgctcaacga gtacgagcgg gccgtggtct tcaggctcgg
      841 cagggtgctc ggcaccccca agggccccgg gctcatcatc atcatcccgc tcgtggacag
      901 gttcatcagg ctcagcatga ggaccatcgt tcaggacgtc ccgccccagg acgtgatcac
      961 cagggacaac gtgacggtca aggtcaacgc cgtcgtgtac ttcagggtga tcgaccccct
     1021 caaggcggtc atcgaggtgg agaactacct gtacgcgacg agccagctca gccagacgac
     1081 cctgcgctcg gtgtgcggcc aggcggagct ggacgaactc ctctccaaga gggaggaggt
     1141 gagcctccac atccagagca tcctcgacag gcagaccgat ccctggggca tcaaggtgtc
     1201 ggcggtcgag ctcaagcaca tagacctgcc cgaggagatg cagcgggcca tggcgcgcca
     1261 ggccgaggcc gaaagggagc ggcgcgccaa ggtgatcaac gccgagggcg agtaccaagc
     1321 cgcccagagg ctcttcgagg cggctcaggt catagccaag gagcccatcg ccgtgcagct
     1381 caggtatctc cagaccgttc gggagatctc gtccgagggt tcgaccaaca cgatcatccc
     1441 cctgcccata gagctcctgg ggccgtttct gaagaagtga acaggggtgc cgggccggcc
     1501 atggccgtgc ttacgagcag gggcaacaag gtctacaagg ccatctgccg cctgaagagg
     1561 agcagggacg tcatggtgct cgagggcagg cgcctggtcc aggacgccct ttcgcggggg
     1621 ctcaggccaa ggacgatcgc ggtgagcgcc tcgttcctcg gcgctcacgc cctgcccgcg
     1681 agcccggatg cggtcttctc cgacgcactg ttcgccaccc tcgcggagac cgagacgagc
     1741 cagggcctgc tggcgctgtt cgacgtgccg tgggccagga tggaggaggt cctcggccag
     1801 gaccgcatcg tggtcttgga cggcctccag gacccgggga acgtggggac cgtgataagg
     1861 accgccgagg cgttcggttt caggggcgtc gtgatcttgc cgaacaccgc gagccctttc
     1921 tcccccaagg cggtgagggc gtccatgggg tcgtgcctgg gggtcgccgt cgcgagaggc
     1981 acccacgagg agatcgtggc gtcttcgcat aggctggtgg cgctcaccat agacggggac
     2041 gaaaccccgg ccccggccct cttcgagggt cggttcgccc tctgcctcgg ccgggaggcc
     2101 tcggggttga gcccggtgct cgcttcccgc gcccatcaca gggtgcgcat ccccatggcg
     2161 gggccgaccg agtcgctgaa cgtggccgtg gcggcaggca tcgtcatggc cgtcgccgcg
     2221 ggggtggtcg ccggcccagg ctgaggacac cccccggcct ctcatgtggg cgtcccgggg
     2281 ggcatgacgc gt
//
LOCUS       DAOSCR010000467         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_30926_length_2285_cov_2.267713, whole genome shotgun sequence.
ACCESSION   DAOSCR010000467 DAOSCR010000000
VERSION     DAOSCR010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30926_length_2285_cov_2.267713"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLT69_10435"
     CDS             complement(<1..587)
                     /locus_tag="PLT69_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ14899.1"
                     /translation="MDLHRLFHPESVAVVGASPHIGGGKLPYYQVLQMAGYKGRLYPV
                     NPKYDSIAGAKVYASLDDLPEPVDLAIASVPYEKALETVEAAARRGVKFIHFFTSGFS
                     EVGNHDLERIMNTLGNDVRKNQVAASLLLTQPGVPFIYYGEEIGMAGIKGLEGDAPLR
                     APMSWTAEKQGSGFTTGQPFRPVSPNAARYNAEAQL"
     gene            943..1560
                     /locus_tag="PLT69_10440"
     CDS             943..1560
                     /locus_tag="PLT69_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14900.1"
                     /translation="MRKHYPSMRIAPPWSAIYRRLGFKAGTTELREGERVHIDRLIQE
                     GIDLVELKGASLVLGIKGLGEGGVLLEGDVLFPSASLAGMLAGCIGALIMAATAGRGI
                     VEAIERSSADDLTRAVVYDAVGSEMADAALDWIVSAVGQDLVRMALRPTRKRFSSGYG
                     DLGLESQQTIWEMLRLEELGIGLTPSMMLVPGKSVTAVAGVVTLA"
     gene            1590..>2285
                     /locus_tag="PLT69_10445"
     CDS             1590..>2285
                     /locus_tag="PLT69_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ14901.1"
                     /translation="MKTRDRIRNRLSRRVLILDGATGTELQARGMPPGVSPELYCAEH
                     PDIIRSVHKGYMLAGADVVYTCTFGANRYKLASYGSADARELNRRLALIAREASGGRA
                     MVAGDIGPTGRLVEPFGDLAFEDAVACFKEQVQGLAQGGVDLFVIETMMDIQEARAAL
                     IAVKETVGDAFTIVTMTYDSSGRTLTGSDCVSALVTLQSLGADAVGCNCSAGPAEMVP
                     LVAAMSPLARVPIV"
BASE COUNT          399 a          674 c          763 g          449 t
ORIGIN      
        1 agttgtgctt ctgcgttgta gcgcgccgcg ttgggcgaca cgggcctgaa cggttggccc
       61 gtcgtgaacc cggaaccttg cttttcagcc gtccagctca tcggtgcgcg cagcggcgca
      121 tcgccttcca ggcccttgat gccggccatg ccgatttctt caccgtaata gatgaacggc
      181 acgccaggtt gggtcagtag caagctcgcc gccacttggt ttttgcggac atcattgccc
      241 agcgtattca tgatgcgttc gaggtcgtgg ttgcccacct ctgaaaaccc ggaggtgaag
      301 aaatggatga acttcacgcc ccttctggcg gccgcctcca cggtctcgag ggccttttcg
      361 tacggcacgg aggctatggc gaggtccacc ggctcgggga ggtcgtcaag ggatgcgtag
      421 accttggcgc cggctatgga atcgtacttc gggttgaccg gatagagcct gcccttgtat
      481 ccagccatct ggaggacctg gtagtagggg agtttccctc ccccgatgtg aggcgaggca
      541 cccaccacgg cgacgctttc cggatggaac agtcggtgca agtccattcg ttctctccct
      601 ctaagttgca cattctgtgt tatgatcagt gtatattacc aaagagaggt ttccgaggct
      661 agcctttata tgcaggcggt catcatggag aaggcgtgcg tgacgccgct cgcgggcgct
      721 ggacgcgccg cccttgaaca ccgggccttc cctctgtgga tgcacgaggc ctcgctcgat
      781 gacatcgagc gcgtgtgtgc gagggatggg gttctcccat ggagggtcga gtgtctttca
      841 ccccttgtcc atgcatgcat ggtcgtgcaa ggcgagccta tcccggcacg atccacttgc
      901 acataccgtc gcgggcgtct agatcataag gggatgatcg gcgtgaggaa acactacccg
      961 tccatgcgta tcgcgccgcc ttggtcggct atctacaggc ggctcggctt caaggcgggc
     1021 accaccgagc tcagggaagg cgagcgggta cacatcgaca ggttgatcca ggagggcatc
     1081 gacctggtcg agctcaaggg ggcgtccctc gtgctcggga tcaagggcct gggggagggc
     1141 ggggtgctcc tcgagggcga tgtgctcttc ccgagtgcgt cgcttgccgg catgctcgcc
     1201 ggttgtatag gcgccctcat catggctgcg acggcaggga gggggatcgt ggaggccatc
     1261 gagcgctctt cggcggacga cctgaccagg gccgtcgtct acgatgcggt gggcagcgag
     1321 atggccgatg cggctctcga ctggatagtg tccgctgtcg gccaggacct cgtcaggatg
     1381 gcgctcaggc ccacgaggaa gagattctcc tccggttacg gcgatctcgg gttggagagc
     1441 caacagacga tatgggagat gctccgcctc gaggagctgg gaatcggcct cacgccttcg
     1501 atgatgctcg tgccgggaaa gagcgtcacg gccgttgcag gcgtcgtgac tttggcttag
     1561 gatgccgaag aaaacatcca ggggatgacg tgaaaaccag agaccgtata cggaacaggc
     1621 tgtcgaggcg tgtgttgatc ttggacggcg cgacgggcac cgagttgcag gcccgaggga
     1681 tgcctcccgg cgtatcgccc gaactctact gtgccgagca cccggacatc attcggtccg
     1741 tccacaaggg atacatgctc gccggggcgg atgtcgtcta tacgtgcacc ttcggtgcga
     1801 acaggtacaa gctcgcatcg tacggttcgg cggacgcgag ggagctgaac cgtcgcctgg
     1861 cgctcatagc ccgagaggcc tcaggcggaa gggccatggt cgcgggtgat atcggcccca
     1921 cggggaggct cgtcgagccg ttcggagacc tcgccttcga ggatgcggtc gcctgcttca
     1981 aggaacaggt ccaagggctc gcccagggcg gggtcgacct cttcgtgata gagaccatga
     2041 tggatatcca ggaggccagg gccgccctga tcgcggtcaa ggagacggtg ggggatgcct
     2101 tcaccatcgt gaccatgacc tacgactcat ccggcaggac gctcacgggc tccgactgcg
     2161 tatcggcact cgtcacgctc cagtccctcg gtgccgacgc ggtcgggtgc aactgttccg
     2221 caggccccgc agagatggtc cccctcgttg cggccatgag ccccctggcg cgtgtgccca
     2281 tcgtc
//
LOCUS       DAOSCR010000468         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31104_length_2275_cov_3.147748, whole genome shotgun sequence.
ACCESSION   DAOSCR010000468 DAOSCR010000000
VERSION     DAOSCR010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31104_length_2275_cov_3.147748"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLT69_10450"
     CDS             <1..1263
                     /locus_tag="PLT69_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14902.1"
                     /translation="LRGQDHPGILRGLRPEKRGKRKSARERRQGPNPGEYEVSFLKRL
                     IATSALCAAVLGLAAAVLAQTAIPQTGFCVEVEPWGEASALFRSDPRWLGGDGASSVD
                     LGGERVLWLFGDSFVDPGGSSSRLNASLVRNSVALQKGKKPESAKMEFFWKMLGNRPR
                     SYFREEDGSWFWPGSGVRLGDILIEFLVEVRRAENPLGFSAEGSKAVIVPNPDDPPSL
                     WAHLRASPAGRRRILVGSACALVEGGYLYAFGSDASGTEVHALRWPVDRAARGDLSSP
                     EWWMGPDAGWASSPRGTLNPRPVFVGAQTEFSIHFEPDLKGFIQVQTLTLANPCLAVR
                     FSPAIERGWTEPACLHDPTRPGKEGIVIYAGKAHKVFEGASLAFTYVVNTLDAETLLN
                     DRELYYPVVLKGHLSPCSRPTEVRTPTP"
     gene            1283..>2275
                     /locus_tag="PLT69_10455"
     CDS             1283..>2275
                     /locus_tag="PLT69_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14903.1"
                     /translation="MGDEASQRLETAAIVIVEAICAFEGALRRLHPASIGALREGLLL
                     RAGGLASVTKTLSAEAAGARSDEARCMADAAGLLMDAISLFGNGDDPARAFLAALQSV
                     RPFCRAHELLFPLASSIPTLRSLFEEGAAACTPGAPRGGRPGPEDLLVHKTSLGDDPY
                     GRGGYSLFVPSSGRGRPVPLVVAMHGGSGHGRDFLWTWLREARSRGFILMAPTSSGRT
                     WSLDDIARDAERVAGHIDEVCSKHQVDRTRVLLTGVSDGGTFALGLGLGDWLSAPDIA
                     PVACVLPRVDLKAARSRRILWVHGALDWMFPVGTAARACEELSRAGCDVRLRIIQ"
BASE COUNT          356 a          733 c          807 g          379 t
ORIGIN      
        1 ttacgtggac aagaccaccc aggcatactg agaggcctgc ggcccgagaa acgggggaag
       61 aggaagtccg ctcgtgaacg aaggcagggc ccgaacccgg gagaatacga ggtgtctttc
      121 ctgaagaggc tcatcgcgac gtcggctttg tgtgcggccg tgctcggcct tgcggcggcc
      181 gtcctcgcac agaccgccat accccagacg gggttctgcg tcgaggtcga accatggggg
      241 gaggccagcg cccttttcag gtcggacccg aggtggctgg gcggtgacgg ggcgtcgtct
      301 gtcgacctgg gtggggagag ggtcctctgg ctcttcggcg acagcttcgt ggatccgggc
      361 gggtcttcct cgcgcctgaa cgcatccctc gtgcgcaaca gcgtcgccct gcagaagggc
      421 aagaagccgg agtcggcgaa gatggagttc ttctggaaga tgctcggcaa caggcccagg
      481 tcgtacttca gggaggaaga cggctcgtgg ttctggcccg ggtcgggcgt caggctcggg
      541 gatatcctca tcgagttcct cgtggaggtg cgccgggcgg agaaccccct gggtttctcc
      601 gcggaggggt cgaaggcggt catcgtgccg aatcccgacg acccgccttc gctctgggcg
      661 catctgcggg cctcaccggc gggtcgccgc cggatactcg tgggttccgc gtgcgccctg
      721 gtcgaggggg gatacctcta cgcctttggg agcgacgcgt cgggcacgga agtccacgcc
      781 ctcaggtggc ccgtggatcg ggcagctcga ggcgacctct cgtcgcccga atggtggatg
      841 ggaccagatg ccgggtgggc ctcctccccg agggggaccc tgaacccccg gcccgtgttc
      901 gtgggcgccc agaccgagtt ctccatccac ttcgagcccg atctcaaggg attcatccag
      961 gtgcagaccc tcacgctcgc caacccgtgt ctcgccgtca ggttctcccc ggccatcgag
     1021 cgggggtgga cggaacccgc ctgtctccac gaccctacga ggcccgggaa ggagggtatt
     1081 gtcatctacg ccgggaaggc gcacaaggtt ttcgagggtg cgtccttggc gttcacctac
     1141 gtggtgaaca ccctggatgc agaaaccctg ttgaacgaca gagaactcta ctaccccgtg
     1201 gtgctcaagg ggcacctgtc gccctgcagt cggcccacgg aggtacgaac accgacaccc
     1261 tagagagggg gaggcccaaa ccatgggaga cgaggcgtcc cagagactgg aaacagcggc
     1321 catcgtgatc gtggaagcga tctgcgcctt cgagggggcg ctgaggaggc ttcaccccgc
     1381 atcgatcggg gcgctcaggg agggcctcct gttgcgcgca ggcggcctcg catccgtgac
     1441 caagaccctc tccgcggagg cggcgggggc ccgctcggac gaggccaggt gcatggccga
     1501 tgcagcggga ctcctcatgg acgcgatctc cctgttcggc aacggggacg acccggcacg
     1561 agcctttctc gcagcgcttc agtcggtgag gccgttttgc agggcccacg aactcctctt
     1621 ccccctcgcc tcgagcatcc ccaccctcag atccctgttc gaggaggggg ctgccgcatg
     1681 cacgccgggg gcaccccgtg gtggtcgccc gggccctgaa gacctcttgg tccacaagac
     1741 ctcgctcggg gatgacccct acggccgcgg cgggtactcc ctgttcgtgc cctcgtcggg
     1801 tcgagggcgg cctgtgccgc tcgtggtggc catgcacggc ggctccggcc atgggaggga
     1861 tttcctgtgg acatggctca gggaggccag gagcaggggg ttcatcctca tggccccgac
     1921 ctcctcgggc aggacctggt cgctcgacga cattgccagg gatgccgaga gggtcgcggg
     1981 ccacatagac gaggtatgct ctaagcacca ggtggacagg acacgggtgc tcctcaccgg
     2041 ggtatcggac ggcgggacct tcgccctcgg cctcggcctg ggggactggc tctccgcccc
     2101 ggacatcgcg ccggtggcat gcgtgctgcc ccgggtggac ctgaaggccg cgcggtcaag
     2161 gcgcatcctg tgggtgcacg gcgcgctcga ctggatgttc ccggtcggca cggctgccag
     2221 ggcctgcgag gagctctcga gggcgggctg tgacgtcagg ctcaggatca tccag
//
LOCUS       DAOSCR010000469         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31241_length_2268_cov_3.627655, whole genome shotgun sequence.
ACCESSION   DAOSCR010000469 DAOSCR010000000
VERSION     DAOSCR010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31241_length_2268_cov_3.627655"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..655
                     /locus_tag="PLT69_10460"
     CDS             98..655
                     /locus_tag="PLT69_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ14904.1"
                     /translation="MTQEQKINDEQGENTSSLMQEQGQGTGEPSAPAEEGPSYEELKA
                     QVDEYKDKYLRALAEMDNMRKRQQRQHEEVVKYGKEAVLKDFLLVYDALEKSIVTARD
                     LYPDDEPFITGLQMIERLLLDTLKRHGVEPIQTQDAAFDPRYHEAMMQVSREDLAAGT
                     VVDEVEKGFMLNDRVLRPAKVTVAA"
     gene            684..>2268
                     /gene="dnaK"
                     /locus_tag="PLT69_10465"
     CDS             684..>2268
                     /gene="dnaK"
                     /locus_tag="PLT69_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOJ14905.1"
                     /translation="MGKILGIDLGTTNSCMAIMEGGEPKVIPNAEGARTTPSVVAFTA
                     SGERLVGQVARRQAITNSEKTLYAIKRLIGRKFDDGEVQRDIKVMPFKIVRADNGDAA
                     IQIEERRYAPPEISAMVLQKLKMDAEAYLGEKIEEAVITVPAYFNDSQRQATKDAGRI
                     AGFEVKRIINEPTAAALAYGLDRKKEEKVAVFDLGGGTFDISILEIGDGVFEVKSTNG
                     DTHLGGEDIDNRIIDYLVSEFRKETGVDLSKDRMALQRLKEAAERAKHELSSMMETEI
                     NLPFITADQSGPKHLNMKLTRAKLENLVEDIIAKLEEPCRKALADAGLTPKDIDEVIL
                     VGGQTRMPRVVQKVKEIFGKEPHKGVNPDEVVAIGAAIQGGVLGGEVKDVLLLDVTPL
                     SLGIETLGGVFTKIIERNTTIPTKKTQIFSTAEDNQNAVTIRVFQGERPMAADNKLLG
                     QFDLVGIPPAPRGIPQIEVSFDIDANGILHVSAKDKATGKEQSIRITSSSGLTEEDIN
                     RMIREAEEHESEDRRRKELV"
BASE COUNT          532 a          671 c          740 g          325 t
ORIGIN      
        1 ggcgtgatcg gtccaatacg gatggattac tcgcgggtga tcccgctggt ggggtacatg
       61 gcacacatct tgagcgagct cctggaggaa atgtgacatg acgcaagagc agaagataaa
      121 cgatgaacag ggagaaaaca cctcatccct gatgcaggaa caaggccaag gtaccggtga
      181 gccgtccgcc cctgccgaag aagggccttc gtacgaggaa ctcaaggcgc aggtggacga
      241 gtacaaggac aagtacctca gggcgctggc ggagatggac aacatgcgca agcgccagca
      301 gcgccagcac gaggaggtcg tcaagtacgg caaggaggcg gtcctcaagg acttcctgct
      361 cgtctacgac gcgctcgaga agtccatcgt taccgccagg gacctctatc ccgacgacga
      421 gccgttcatc acggggctcc agatgataga gcgcctgctc ttggatacct tgaaaagaca
      481 cggggtggaa cctatacaaa cccaagacgc agcgttcgac ccgagatacc acgaggccat
      541 gatgcaggtc tcgagggaag accttgcggc cgggacggtc gtggacgagg tcgagaaagg
      601 cttcatgctc aacgacaggg tgctgaggcc tgcgaaggtc acggtggctg cgtgacgata
      661 cacttttcgg gaggatagtt cacatgggaa agatactagg catcgacctt ggcacaacca
      721 attcctgcat ggccatcatg gaaggcggcg aaccgaaggt gatccccaac gccgaaggcg
      781 cgaggacgac cccgtcggtg gtcgcgttca ccgcgagcgg cgagcgcctc gtcggccagg
      841 tggcgaggcg gcaggccatc accaactcgg aaaagacctt gtacgccatc aagaggctca
      901 tcggcaggaa gttcgacgac ggcgaggtcc agcgtgacat taaggtcatg cctttcaaga
      961 tcgtgcgtgc cgacaacggc gacgcggcga tccagatcga ggaaagacgc tacgcgccgc
     1021 cagagatatc ggccatggtc ctccagaagc tcaagatgga cgccgaggcc tacctgggcg
     1081 agaagatcga ggaggcggtc atcacggtgc cggcttactt caacgactcg cagcggcagg
     1141 ccaccaagga cgcaggcagg atagccgggt tcgaggtgaa gaggatcatc aacgagccca
     1201 ccgcggcggc gctcgcctac ggtctcgaca ggaagaagga ggagaaggtc gcggtcttcg
     1261 acctcggcgg cgggacgttc gacatctcca tcctcgagat cggggacggc gtgttcgagg
     1321 tgaagtcgac caacggcgac acgcacctgg gcggcgagga catcgacaac aggatcatcg
     1381 actacctcgt gagcgagttc cgcaaggaga ccggggtcga cctcagcaag gaccgcatgg
     1441 ccctccagcg cctgaaggag gcggcggaga gggccaagca cgagctttcg tccatgatgg
     1501 agaccgagat caacctcccc ttcatcacgg cggaccagtc ggggcccaag cacctcaaca
     1561 tgaagctcac gcgggccaag ctcgagaacc tcgtcgagga catcatcgcc aagctcgagg
     1621 agccctgccg caaggctctt gccgacgccg gtctcacgcc caaggacatc gacgaggtga
     1681 tcctcgtggg cggccagacc cgcatgcccc gggtggtgca gaaggtgaag gagatcttcg
     1741 gcaaggaacc ccacaagggc gtcaaccccg acgaggtcgt ggccataggc gcggcgatcc
     1801 agggcggcgt gctcgggggc gaggtcaagg acgtcctgct cttggacgtg acgcccctct
     1861 ccctggggat cgagaccttg ggcggcgtgt tcaccaagat catcgagcgc aacaccacga
     1921 tccccacgaa gaagacccag atcttctcga ccgcagagga caaccagaac gcggtcacca
     1981 tcagggtatt ccagggggag aggcccatgg cggccgacaa caagctcttg ggccagttcg
     2041 acctcgtggg cattccgccc gccccgaggg gtatccccca gatcgaggtg agcttcgaca
     2101 tcgacgccaa cggtatcctg cacgtctcgg ccaaggacaa ggcgacgggc aaggaacagt
     2161 ccatccgtat cacgagctcc tccggtctga ccgaggagga catcaaccgg atgatccgtg
     2221 aggcggagga gcacgagagc gaggaccgca ggcgcaagga gctcgtcg
//
LOCUS       DAOSCR010000470         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31428_length_2258_cov_2.704948, whole genome shotgun sequence.
ACCESSION   DAOSCR010000470 DAOSCR010000000
VERSION     DAOSCR010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31428_length_2258_cov_2.704948"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLT69_10470"
     CDS             <1..509
                     /locus_tag="PLT69_10470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ14906.1"
                     /translation="AVITRCSNNYGPYQFPEKLIPLMVVNALNDKDLPVYGDGRNVRD
                     WIHVEDHCRGIVVVAERGRTGEVYNLGGDSERENIEIVRAICRRLGKPETLIRFVKDR
                     PGHDFRYAMDHSKISRELGWEPEIGFEEGLDSTIAWYLENRRWWERIISGDYRQYYER
                     MYGSRERL"
     gene            506..1402
                     /gene="rfbD"
                     /locus_tag="PLT69_10475"
     CDS             506..1402
                     /gene="rfbD"
                     /locus_tag="PLT69_10475"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOJ14907.1"
                     /translation="MRFLVTGAAGMLGSEVVGALESLGHEVWATDVADNEDPLDITRE
                     DRVDAAIRSFRPHWVVNCAAYTNVDGAEDDEEAAFLLNARGPETIARSCRRHGARLLH
                     MSTDYVFDGTKDTPYREDDLPNPLNVYGASKLAGEEAVRHVHGGSLIVRTQWLIGANG
                     PNFVSKIIDAARGRDRLEVVNDQYGSPTFAADLARALCLLMEKGAQGIYHVCNRGVTT
                     WYELASRALAYAAIATPVVPVPTSAIARKARRPAYSALSTVKFSQETGRLMPLWQCSL
                     EAFLHEYLGRPCRNAPTGGSQG"
     gene            1532..2155
                     /gene="lepB"
                     /locus_tag="PLT69_10480"
     CDS             1532..2155
                     /gene="lepB"
                     /locus_tag="PLT69_10480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ14908.1"
                     /translation="MRPDRTKNVLKDWVEALIVAFVIAMVIRAFVLQAYRIPSSSMED
                     TLLKGDHILATKFNYGITVPFTTKKIWGADRIPKRNDIVIFTFPVNHSMDFVKRVIGL
                     PGDVIEVRDKKVYVNGKRYEIPQEKYTDPFSLTQGPGRVRDFFGPVKVSPGHVFVMGE
                     NRDQSYDSRFWGQVPIENIKGKALIIYFSWTSERPWVRYWRVGRLIH"
BASE COUNT          495 a          636 c          743 g          384 t
ORIGIN      
        1 atgcggtgat cacaaggtgt tccaacaatt acggcccgta ccagttcccc gagaagctca
       61 tccctctcat ggtcgtcaac gccctcaacg acaaggatct gccggtgtat ggcgacggca
      121 ggaacgtgag ggactggatc cacgtggagg accactgcag gggcatcgtg gtggttgcgg
      181 aacgtggccg cacgggtgag gtctacaatc tggggggtga ctccgagagg gagaacatag
      241 agatcgtgcg ggccatatgt cgccgcctag gcaagcccga gacgctcatc aggttcgtga
      301 aggaccgccc cggccatgat ttcaggtatg ccatggacca ctcgaagatc tcgcgggagc
      361 tcgggtggga accggagatc gggttcgaag aggggctcga cagcaccatc gcctggtatc
      421 tcgagaacag gcggtggtgg gaaaggatca tatcaggcga ttaccgccag tactacgaga
      481 ggatgtacgg gagcagggag cgcctatgag gttcctggtc acgggagcgg cgggaatgct
      541 cggaagcgag gtggtcgggg cgctcgagtc cctcggccac gaggtctggg ccacggacgt
      601 ggccgacaat gaggacccgc tcgacatcac cagggaagac cgggtggacg ccgcgataag
      661 gtctttcagg ccgcactggg tggtcaactg tgcggcttac acgaacgtcg acggggcgga
      721 ggacgacgag gaggccgcct tcctcctgaa cgcgaggggg cctgagacga tcgcccgttc
      781 ctgcaggagg catggggcgc gcttgctgca catgagcacc gattacgtgt tcgacgggac
      841 caaggacact ccttacaggg aagacgatct cccgaacccc ctgaacgtat acggagcgag
      901 caagctcgcc ggcgaggaag cggtcaggca cgttcacggg ggcagcctca tcgtcaggac
      961 acagtggctc atcggcgcca acgggcccaa cttcgtgtcc aagatcatcg atgcagcccg
     1021 tgggagagac cgcctcgagg tggtgaacga ccagtacggc agccccacgt ttgccgccga
     1081 cctggcgagg gccctgtgct tgctgatgga aaagggggcg caggggatct accacgtgtg
     1141 caacaggggg gtgacaacct ggtacgagct ggcatccagg gcgttggcgt acgcggccat
     1201 agcgacaccg gtggtcccgg ttcccacctc cgcgatcgcc aggaaggcca ggaggcccgc
     1261 atactcggcg ctttccaccg tgaagttctc gcaggaaaca ggcaggctca tgcccctatg
     1321 gcaatgctcc ctcgaggcgt tcctgcacga gtacctcggg cgaccctgca ggaacgcgcc
     1381 tacgggggga agtcagggct gatggttgga tctgtggagc gaaaaacgtc ttttgcaaac
     1441 cgcccgattc tgttaaacag ggcaaggtgc ggcggatgga tatacgcggt agtgcgaccc
     1501 ttcggaacat gacccagaga ggggggatcg attgagaccg gacaggacga agaatgtcct
     1561 caaggactgg gtggaagcgc tcatcgtagc cttcgtgatc gccatggtca ttcgcgcctt
     1621 tgtcctgcag gcgtacagga tcccatcctc ttccatggaa gacacgctcc tcaaggggga
     1681 tcacatcctg gcgacaaagt tcaactatgg tatcacggtg cccttcacca ccaagaagat
     1741 ctggggtgca gaccgtatac ccaaaaggaa cgacatcgtc attttcacgt tccccgtgaa
     1801 ccactccatg gatttcgtca agagggtcat cggcctgccc ggtgacgtca tcgaggtccg
     1861 cgacaagaag gtgtacgtca acggaaagag atacgagatc ccgcaggaga agtacacgga
     1921 cccattctcc ctgacccagg gcccgggcag ggtgagggac ttcttcggcc cggtaaaggt
     1981 cagccctggc cacgtgttcg tcatgggcga gaacagggac cagagctacg acagcaggtt
     2041 ctggggccag gtgcccatcg agaacatcaa gggcaaggcc ctgatcatat acttctcgtg
     2101 gacctccgag agaccttggg tcagatactg gcgtgtaggg agactcatcc actagcagag
     2161 ggttctcatg aaacaggcgc ctacagaccc tgcgatcata cagggccttg tcgagaggat
     2221 cgcggaagaa ctctcgccca tggtgagcag cggcgcgc
//
LOCUS       DAOSCR010000471         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31447_length_2257_cov_2.983651, whole genome shotgun sequence.
ACCESSION   DAOSCR010000471 DAOSCR010000000
VERSION     DAOSCR010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31447_length_2257_cov_2.983651"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLT69_10485"
     CDS             <1..594
                     /locus_tag="PLT69_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOJ14909.1"
                     /translation="FENFLRGESLVITRDNFGCGGAGTHLCNVQTRSRQDFIHFLADT
                     EGLKRSHELMGRWIDSRKPYIQQHPFLVMGPLRREKQEYLKTVTFFVNPDQLSILIIG
                     ANYDASPDDPQPVIAPFGSGCMELLPLFNDLDTPQAIIGATDIAMRQYLPRDLLAFTV
                     TRPMFERLCALDEGSFLFKPFLKNLRKARKTLSTQGS"
     gene            727..1302
                     /locus_tag="PLT69_10490"
     CDS             727..1302
                     /locus_tag="PLT69_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ14910.1"
                     /translation="MTLNELVERNRSYRRFYEDEPVKPETLRELVGLARLSASAANLQ
                     PLRYLLSWEPKKNGLIFPHLGWAAYLEDWPGPAAGERPAAYIVILGDTSVAKTFGCDH
                     GIAAQSILLGAVERGLGGCMLASIQRSRLREALRIPARYEILLVIALGRPREEVVIED
                     VEDGGSIEYYRDGKGVHHVPKRRLDDLILSL"
     gene            1325..1813
                     /locus_tag="PLT69_10495"
     CDS             1325..1813
                     /locus_tag="PLT69_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOJ14911.1"
                     /translation="MDVCEFLRAHGIACERYDHEAVFTCEQAEGLGIPTDAAGTKNLF
                     LRDRKGRRHFLVTVSAEKTVDLKALEEILGARGLSFASEDRLERHLGLQAGSVTILGV
                     LNDRQGSVEVIVDEDLWKCTAIQCHPMVNTSTLAVSVDGIRRFLELTGHEPRIIRIPE
                     RA"
     gene            1997..>2257
                     /locus_tag="PLT69_10500"
     CDS             1997..>2257
                     /locus_tag="PLT69_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOJ14912.1"
                     /translation="MPGGLGGMDERTGQAARTDDTSLLAGRAIAGDRESFGKLMAAYH
                     GRIFAMVYGRVMSRMDAEDITQEVFASAFRGIGTLRDPELFRP"
BASE COUNT          425 a          713 c          759 g          360 t
ORIGIN      
        1 ttcgagaact tcctcagggg cgagagcctc gtgatcaccc gggacaactt cgggtgcggg
       61 ggcgcgggca cccacctgtg caacgtgcag acgcgctcga ggcaggactt catccatttc
      121 ctggccgaca ccgaggggct caagagatcg cacgagctca tggggcggtg gatcgacagc
      181 cggaagccct acatccagca gcatcccttc ctggtcatgg gcccgctgag gcgggagaaa
      241 caggagtacc tcaagacggt caccttcttc gtcaaccccg accagctgag cattctcatc
      301 atcggggcca actacgacgc ctcgccggac gatccccaac ccgtgatcgc gcccttcggc
      361 tccggctgca tggagctcct gcccctcttc aatgacctgg atacgcccca ggccatcatc
      421 ggggccaccg acatcgccat gcggcagtac cttccccgcg acctgctggc cttcaccgtc
      481 acccggccca tgttcgagag gctttgcgcc ctggatgaag ggagctttct cttcaagccc
      541 ttcctgaaga acctcaggaa ggccaggaaa accctgtcga ctcaaggatc atagccggca
      601 acgggccgcc cgggcacccg tgggcggccc cggcggcaag cctccggtcc ggcgctcgaa
      661 ccccggggtc caagccgggc tcgaggggac cgcacgcggg gccgggggga caagggagga
      721 aaggacatga cattgaatga actggttgag aggaaccgat cgtaccggag gttctacgag
      781 gacgagccgg taaaacccga gacgctcagg gagctggtgg gcctggcgcg gctctccgcc
      841 tcggcggcca atctccagcc cctgaggtac ctcctctcgt gggagcccaa gaagaacggg
      901 ctcatctttc cgcacctggg gtgggcggcc tacctcgagg attggccggg accggctgct
      961 ggagagcggc ctgccgccta catcgtgatc ttgggcgaca cgagcgttgc aaagaccttt
     1021 ggctgcgacc acggcatcgc tgcgcagagc atcctgcttg gcgccgtgga gcggggcctg
     1081 ggcggctgca tgctcgcgtc catccagcgc agcaggctca gggaggccct gcgcatcccc
     1141 gcgcgctatg agatcctcct ggtcatcgcc ctgggcaggc cccgagaaga ggtagtcatc
     1201 gaggatgttg aggatggcgg gagcatcgag tactaccggg acgggaaagg agtccaccat
     1261 gtgccaaagc gaaggctcga cgacctcatc ctgtccctgt gaggccgctg gcggaaagag
     1321 gctgatggac gtctgcgagt tcttgagggc ccacggcatc gcctgcgagc gctacgacca
     1381 cgaggcggta tttacctgcg aacaggccga gggcctgggc attcccacgg atgcggctgg
     1441 gaccaagaac ctcttcctgc gcgacaggaa ggggcgcagg cacttcctgg tcacggtgag
     1501 cgcagagaag accgtggatc tcaaggcgct cgaggagatc cttggcgccc gggggttgag
     1561 cttcgcgtcc gaggaccggc tcgagcggca cctggggctc caggccggat cggtcaccat
     1621 cctgggcgtt ctcaacgacc gccagggcag cgtcgaggtg atcgtggacg aagatctgtg
     1681 gaagtgcact gccatccagt gccatcccat ggtgaacacg agcacgctcg ccgtgtccgt
     1741 ggacggcatc aggcgcttcc tcgagctcac cgggcacgag cccaggatca ttcgcatccc
     1801 ggagcgcgca tgacagcacc cgcggagagg ccctgcggag tatttcctgg aattcacttt
     1861 ttgcctgtag acaaaaccat cggcaacccc gtctttgctc acaaaagcag ccggtttcaa
     1921 gggccgccgc ggggaggcgt ggcgctcacg gatgcacggc cggccttgag gggagtgaag
     1981 gacgggagac tcagtgatgc ccggagggct cggcggcatg gatgagcgga caggccaggc
     2041 ggcgcgcacg gatgatacga gcctgctggc gggcagggcg atcgccgggg acagggagtc
     2101 gttcgggaag ctcatggccg cctaccacgg gaggatattc gccatggtct acggccgggt
     2161 catgtctcgc atggatgccg aggacataac gcaagaggtc ttcgcaagcg cgttcagggg
     2221 catcggcacg ctcagggacc ctgagctgtt caggccc
//
LOCUS       DAOSCR010000472         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31611_length_2248_cov_3.331509, whole genome shotgun sequence.
ACCESSION   DAOSCR010000472 DAOSCR010000000
VERSION     DAOSCR010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31611_length_2248_cov_3.331509"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1943
                     /locus_tag="PLT69_10505"
     CDS             336..1943
                     /locus_tag="PLT69_10505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14913.1"
                     /translation="MVRVTCLGSAGGVTGANFLVETPSGVKVLVDCGLFQGGRRMEMR
                     NWREWDFEPDRVAALLLTHAHIDHSGRIPKLVRDGFKGWILATGPTVELCSIMLLDAA
                     HIQEVDAAWQTRKNRRRAQEGVAPLYTTADAEDSLRCFVTVEEDEPVDVAPGMRARFR
                     NAGHILGSAILELWVRENDHEIKIVFSGDLGKSNKLILKDPAEIFNADYLFVESTYGD
                     RLHKSLEESKAELIEAIRHAYDNREKVIIPAFAVERTQELIYILSEFARNKILPDMPV
                     YIDSPLAIKATEIFRRNKEHYDEEAMAVVNGGADPFSLPNLRYSETTEDSMAINRREG
                     PAIVIAGNGMCTAGRIRHHLKHNLWREGASIVIVGYQANGSTGRKIVEGHKTVRIFGE
                     DVAVKARVFTIGGFSAHADRDDLLAWVSNFAESMPRVFVVHGEPAVADAFARLVTERT
                     GMATHVPRWRERLVLKAREFTSEVPAREEGLADMRSDMLAVISDVERRLAQLRVKIDQ
                     PAYLGRITEDEVDRLRYIEEELGEILA"
BASE COUNT          451 a          638 c          789 g          370 t
ORIGIN      
        1 gaaccgtctg tgaggagaag gctgcgccgg accatgtcga ccccggcgac tctttgtgag
       61 gcccgaccct gtgaatgccg tcgagaggcc ggtctcggcc tggagccacg agagaccgga
      121 ggtgtcgagc ggtctcggcg ttgcctgagc gggatgtcgt gcaccctcga ggtgtcgggt
      181 tgcacgaaac tgtggtaagg atttgtgggg tgttatagga aaagggctta cgtgaccgga
      241 aacccttccg atggagtgga tggagggcga aggaagcggg agcgaagggg atgccggttt
      301 ttgcctgaga cgtgcgagca cgaaaggagc gaatcatggt cagggttacc tgcctggggt
      361 ctgcaggggg cgtcaccggg gcgaatttcc tcgtggagac cccctcgggg gtgaaggtcc
      421 tcgtcgactg cggtctcttc cagggcggca ggcggatgga gatgcgcaac tggcgcgagt
      481 gggacttcga accggacagg gtcgcggccc tcttgctcac ccatgcccac atcgaccaca
      541 gcggccgcat cccgaagctc gtgagagacg gtttcaaggg atggatcctc gccacaggcc
      601 cgacggtgga gctctgttcc atcatgctcc tggacgcggc ccatatccag gaggtcgacg
      661 ccgcctggca gaccaggaag aacaggaggc gggcgcaaga aggggtcgcg cccctgtaca
      721 cgaccgcgga cgccgaggac agcctgagat gcttcgtgac cgtggaagaa gacgagcccg
      781 tcgacgtggc gcccggcatg agggcgcgct tccgcaacgc gggccacatc ctgggatcgg
      841 ccatcctcga gctctgggtc agggagaacg accatgagat caagatcgtg ttctcgggcg
      901 acctggggaa gtccaacaag ctcatcctca aggaccctgc agagatcttc aacgcagact
      961 acctcttcgt ggaatcgacc tacggggacc gtctccacaa gtcgctcgag gagagtaagg
     1021 ccgagctcat cgaggcgatc cggcacgcgt acgacaacag ggagaaggtc atcatccccg
     1081 cgttcgcggt ggagaggacc caggagctca tctacatcct gagcgagttc gccaggaaca
     1141 agatcctgcc ggacatgccc gtgtacatag acagcccgct cgccatcaag gccacggaga
     1201 tcttcaggag gaacaaggaa cactacgacg aggaggccat ggccgtcgtc aacggcgggg
     1261 ccgatccctt ctcgctcccg aacctgcgct acagcgagac caccgaggat tccatggcca
     1321 taaaccgccg cgagggcccg gccatagtga tagccggaaa cggcatgtgc accgcgggca
     1381 ggatcaggca tcacctcaag cacaacctgt ggcgggaagg tgcgagcatc gtcatcgtgg
     1441 gataccaggc caacgggtcc acggggagga agatcgttga gggccacaag acggtcagga
     1501 tcttcggcga ggacgtggcc gtgaaggcaa gggtcttcac catcggcggc ttctccgccc
     1561 atgccgaccg ggacgacctc cttgcctggg tctcgaactt tgcggagagc atgccacgcg
     1621 tgttcgtggt ccacggcgag ccggcggtgg ccgatgcgtt cgcgaggctc gtgaccgaac
     1681 gcaccggcat ggcgacccat gtccccaggt ggcgggagag gctcgtgctg aaggcgcgcg
     1741 agttcacgag cgaggtcccc gcccgggaag aggggctcgc cgacatgaga tccgacatgc
     1801 tcgccgtgat ctccgacgtg gagaggcggc tggcccagct gagggtgaag atagaccagc
     1861 ctgcctacct cggccggata acggaggacg aggtggacag gctgcgctac atcgaggagg
     1921 agctcggtga gatcctcgcc tgagagggcc gtcccgtccc gggcgaaggt cgcctggacg
     1981 cggcatggtg cacgaggtcc taggcgtccg tggtctgcgt ggtcggtatc atggtggacg
     2041 aagggggtga atctccgtga tctggcctgc atctcgctgg ggatccgaga aagaggaagc
     2101 agagaccatc cgagggtgcg acggtcgcac ggagaggacc gggtgggtgg gtcggccgca
     2161 tcgttcgtgt acgcaaagac ccgatggttt ttctttggtt catgacaact ttagggggtt
     2221 agatcgtaac attttgagga gcagtttg
//
LOCUS       DAOSCR010000473         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31684_length_2244_cov_3.020101, whole genome shotgun sequence.
ACCESSION   DAOSCR010000473 DAOSCR010000000
VERSION     DAOSCR010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31684_length_2244_cov_3.020101"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1313
                     /locus_tag="PLT69_10510"
     CDS             <1..>1313
                     /locus_tag="PLT69_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOJ14914.1"
                     /translation="LTNVFRRLGRICRFYGSSPVFICCSATVANPKEHAENLLEREVE
                     LIDKSGAPRASKTFILYNPPVVNRELGIRQSALAPARAMATDLIENRIQTIVFATSRL
                     NVEVLTKYLKDSFRERVPKGLGMVAGYRGGYLPNLRRQIEQGLRERSILGVVSTNALE
                     LGIDIGELEACIMAGYPGSIASTWQQAGRAGRRTGHSLAVLIARSNPMDQFVVEHPDF
                     FFTRSPEHCRVNPDNLLILLHHVKSAAFELPFEDGERFGREDLVEILSYLEEKGVLHR
                     VEGRWHWSAESYPADEVSLRTVNPENVVVVDTTETGNHRVIAEVDWDSAFTTVHDEAI
                     YMVESRQYHVDKLDLDRKKAYVHEVDVDYYTDAMTYTNVRVLDEFGTMTRERVIVEHG
                     EVQVVRKVVGYKKIKFYTGENVGYGDVNLPQKEMHTTSYWFTIPW"
     assembly_gap    1314..1323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1324..1765
                     /locus_tag="PLT69_10515"
     CDS             <1324..1765
                     /locus_tag="PLT69_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOJ14915.1"
                     /translation="LDTLGLGREEVIDGLAGLAYCLHHIAAMLVMADIRDLDRCIGDK
                     SGQWFVRYGDDLRRITMAPEASVKVMENAFDPTVFIFDAYPGGIGFSELLFSKHDLML
                     SSAASLIGSCPCEHGCPMCVGPVLEVGPRAKDASLMILGLVGRP"
     gene            1762..>2244
                     /locus_tag="PLT69_10520"
     CDS             1762..>2244
                     /locus_tag="PLT69_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14916.1"
                     /translation="MSFRDRLERITGDTRGARPDTEVRPGIGELRKRIEEIMSRRGGA
                     AHAQRPEVLRPRTTLEEAVGGEEVENSQGRFFSVQSVFGPSARHGHALIGELAELDGE
                     LAALLAAENSICGCSSGDALFIDTETTGLAGGTGTFPFLVGLGWFEGGAFVVRQLFAR
                     D"
BASE COUNT          414 a          710 c          755 g          355 t
ORIGIN      
        1 cctcaccaac gtgttcaggc gcttaggccg catttgccgt ttctacggct ccagccccgt
       61 cttcatctgc tgctcggcga ccgtggcgaa ccccaaggaa cacgcggaaa acctcctgga
      121 gcgggaggtc gagctcatag acaaaagcgg cgcgcccagg gcgtccaaga ccttcatcct
      181 ctacaacccg cccgtggtca acagggagct gggcatccgc cagtccgccc tcgccccggc
      241 gcgcgccatg gcgaccgatc tcatcgagaa ccgtatccag accatcgtgt tcgcgaccag
      301 caggctcaac gtggaggtgc tcaccaagta cctgaaggac tcgttcaggg agagggtgcc
      361 caaggggctg ggcatggtcg ccggttaccg tggggggtac ctcccgaacc tgaggaggca
      421 gatcgaacag ggcctgcgcg agcgaagcat cctgggggtg gtgagcacga acgcgctcga
      481 gctggggatc gacatcggcg agctcgaggc gtgcatcatg gccggctacc ccgggtccat
      541 agcgagcacc tggcagcagg cgggccgggc aggccgcagg accggccaca gcctcgccgt
      601 gctcatcgcc aggagcaacc ccatggacca gttcgtggtc gagcacccgg acttcttctt
      661 cacgcgctcc ccggagcact gccgcgtcaa ccccgacaac ctcctcatcc tgctccatca
      721 cgtgaagagc gcggcgttcg agctcccctt cgaggacggc gagaggttcg gcagggaaga
      781 cctcgtcgag atcctctcct acctcgagga gaagggcgtt cttcacaggg tggaaggccg
      841 ctggcactgg tcggcggaga gttacccggc cgacgaggtg agcctgcgca cggtgaaccc
      901 ggagaacgtc gtggtggtgg acaccaccga gacggggaac cacagggtga tagcggaggt
      961 ggactgggac agcgccttca cgacggtgca cgacgaggcc atctacatgg tcgagtccag
     1021 gcagtaccac gtcgacaagc tggacctgga tcggaagaag gcctatgtcc acgaggtgga
     1081 cgttgattac tacaccgacg ccatgacgta caccaacgtg cgggtcctgg acgagttcgg
     1141 tacgatgacc cgggaacgcg tcatcgtgga acacggcgag gtccaggtgg tcaggaaggt
     1201 ggtggggtac aagaagatca agttctacac gggcgagaac gtgggctacg gcgacgtgaa
     1261 cctgccccag aaggagatgc acaccaccag ctactggttc acgataccct ggcnnnnnnn
     1321 nnncctcgac acgctcggac tcggacggga ggaggtcatc gacggcctcg ccggcctcgc
     1381 ctactgcctc caccacatcg ccgccatgct cgtcatggcg gacatccgcg acctggacag
     1441 gtgcatcggg gacaagagcg gccagtggtt cgtacggtac ggggacgacc tcaggcgcat
     1501 caccatggcg cccgaggcgt ccgtgaaggt catggagaac gccttcgacc cgacggtgtt
     1561 catcttcgac gcctaccccg gggggatagg cttttccgag ctcctcttct cgaagcacga
     1621 cctcatgctg tcctcggccg cctccctcat cggttcgtgt ccctgcgagc acgggtgtcc
     1681 catgtgcgtc gggccggtgc tcgaggtggg gcccagggcg aaggacgctt cgctcatgat
     1741 cctcggtctc gtgggaaggc catgagtttc agggaccggc tcgagaggat caccggtgat
     1801 acacggggcg cacgccctga caccgaggtc cgccccggca tcggcgagct caggaagagg
     1861 atcgaggaga tcatgagccg gaggggtggc gccgcccatg cgcagcgccc cgaggtcttg
     1921 cggccccgca cgaccctcga ggaggccgtg ggcggggagg aggtcgaaaa ctcccagggg
     1981 aggttcttct cggtccagag tgttttcggg ccgagtgcga ggcacggcca tgcactgatc
     2041 ggggaactcg ccgagctcga cggggagctt gcagccttgc tcgcggccga gaactcgatc
     2101 tgcgggtgct cgagcgggga cgccctgttc atagacacgg agaccaccgg cctcgcaggg
     2161 ggcacaggca ccttcccctt cctcgtgggg ctcgggtggt tcgagggcgg cgccttcgtc
     2221 gtgcgccagc tctttgcgag ggac
//
LOCUS       DAOSCR010000474         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_31888_length_2235_cov_2.644037, whole genome shotgun sequence.
ACCESSION   DAOSCR010000474 DAOSCR010000000
VERSION     DAOSCR010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31888_length_2235_cov_2.644037"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="PLT69_10525"
     CDS             <1..2153
                     /locus_tag="PLT69_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOJ14917.1"
                     /translation="QLAFAPLIGAMAAGNCAVVKPSSYSPHTSSLIAKILGGCFPEEY
                     IAVIEGGREANQELLKQKFDYIFFTGSVSVGRTVMEAASRVSSALGGELPLLVEVPCG
                     DIEAVGGPALATAIRRMRAGEVIKEPGYDGRFGKVRLFAQADRDLLFDHRLDTGPGRA
                     RRGRPPRKPAVEDACRAAQGAPVTGLSAEQLEAAGWGEGPLAVIAGPGTGKTRVLVER
                     AVRLARKGEGPVLAVTFTVRAAREIAQRLEEGCGRDHGVDVCTFHSLAARILRETGLD
                     FQVMDEGELVRVVEPALDADAGAWVEDLLYRQGTMTPLDREQEGLVRMLRAQGCFTYD
                     GLVDEASRLVRAGAFTPRWRHVMVDEFQDINPVQHEFLKALVRGAASVLVIGDPNQAI
                     YGFRGSSPAAFDDFRKDMPGARTVHIRDTYRFGRPIALAGNAVVGREAVRSLRDGPRV
                     TLVRTPRPWEFVAREAEALSGGLTHAGVHRAAGEYPLSSIAVIVRTKSQALSALKALG
                     EAGIPCDTAYARPLSETRGVAERIALLAGEDVTPYLKKRPARDDELKALARTLAAQDG
                     SSVARRLARMEESGLFDLAPLPETHPLYSYALLFGSDTRGFIDFVRLTRDDAAVTGER
                     VHVITAHAAKGLEFDCVFVAGLSRGVFPLKGSDPVLERNLFYVALTRARQRLYLVCPH
                     EGESPYIGGIPEEAVREIVEKARKPGSAQLLLFE"
BASE COUNT          373 a          711 c          815 g          336 t
ORIGIN      
        1 ttcagctggc cttcgcgccc ctcatcggag ccatggccgc aggaaattgc gcggtagtga
       61 agccctcgtc ctattcgccc catacctcat ccctgattgc gaaaatactg ggcggatgct
      121 ttccggaaga atatattgcc gtcattgagg gcggaaggga ggccaaccag gagcttctga
      181 aacagaagtt cgattacatc ttcttcacgg gaagcgtcag cgtcggaagg accgtgatgg
      241 aagccgcatc cagggtctcc tccgcgctcg ggggcgagct ccccctgctc gtggaggtgc
      301 cctgcgggga catcgaggcg gtcggcgggc cggcactggc gaccgcgata cggcgcatga
      361 gggccggtga ggtgatcaag gaacctgggt acgacggcag gttcgggaag gtgaggctct
      421 tcgcccaggc cgacagggac ctcctcttcg atcaccgtct cgataccggg ccgggcaggg
      481 ccaggcgggg aaggcccccc cggaaaccag ccgtggagga tgcctgccgg gccgcccagg
      541 gcgcgccggt gacaggcctg agcgcggaac agctcgaggc ggccgggtgg ggcgagggcc
      601 cgctcgcggt gatcgcgggt cccgggacgg gaaagaccag ggtgctggtg gaacgcgccg
      661 tgaggctggc gcgaaagggc gaaggccccg tgctcgccgt caccttcacc gtgagggccg
      721 cgcgcgagat agcccagagg ctggaggagg ggtgcggccg cgaccacggg gtggacgtct
      781 gcaccttcca ctccctggcc gcgaggatcc tgcgggagac cgggctggac ttccaggtca
      841 tggacgaggg cgagctcgtg agggtggtgg aaccggcgct ggacgccgac gccggggcgt
      901 gggtcgagga cctcctctac cggcagggga ccatgacccc cctcgacagg gagcaggaag
      961 gcctggtgag gatgctcagg gcacagggct gcttcacgta cgacgggctc gtggacgagg
     1021 cttcccgcct cgtccgggct ggggcgttca cgccccggtg gagacacgtg atggtcgacg
     1081 agttccagga catcaacccg gttcagcacg aattcctgaa agcgctcgtg cggggggccg
     1141 caagcgtcct ggtgatcggc gacccgaacc aggccatcta cggcttcagg gggagcagcc
     1201 cggccgcgtt cgatgatttc cgaaaggaca tgccgggcgc gcgcacggtg cacatccgtg
     1261 acacgtaccg tttcggcagg cccattgcgc ttgcgggaaa cgccgtggtc ggccgggagg
     1321 cggtgcgctc gctcagagac ggccccaggg tgaccctggt gcgcaccccc aggccctggg
     1381 agttcgtcgc gcgcgaggcg gaggccctct cgggcgggct cacgcacgcg ggcgtgcaca
     1441 gggccgcagg cgagtatccc ctgagctcca tcgccgtgat cgtcaggacc aagagccagg
     1501 cgctgtcggc cctgaaggcg ctcggcgagg ccggcatccc ctgcgatacc gcttacgcga
     1561 ggcccctgag cgagacccgg ggggtggcgg aacgtatcgc cctgctcgcg ggggaggacg
     1621 tcacgcccta cctcaagaag agaccagccc gcgacgacga gctgaaggct ttggcgcgca
     1681 ctctcgccgc acaggacggg tcgagcgtgg caaggcggct tgcgcgcatg gaggagtccg
     1741 ggctgttcga ccttgcgccg ctgccggaga cccacccgct ctacagctac gcactcctct
     1801 tcgggagcga cacgcggggg ttcatcgatt tcgtcaggct cacgagggac gacgcggccg
     1861 tcaccgggga gcgggtgcac gtgatcacgg cgcatgcggc caaggggctc gagttcgact
     1921 gcgtcttcgt ggcggggctc tcccgcggcg tgttcccgct caagggctct gaccccgtcc
     1981 tggagaggaa cctcttctac gtcgcgctca cgcgcgccag acaacgcctg tacctggtat
     2041 gcccgcacga gggcgagtcg ccgtacatcg gcgggatccc cgaagaagcg gtccgcgaga
     2101 tcgtcgagaa ggcgaggaag cccgggtctg cccagctgct cctgttcgag tgagacccgg
     2161 ggtgaaaagg tgaggatgcc atggtcacga ccacggagaa ggtagtctac atcgtttccc
     2221 tcctgctcct cgtcc
//
LOCUS       DAOSCR010000475         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32282_length_2216_cov_2.880611, whole genome shotgun sequence.
ACCESSION   DAOSCR010000475 DAOSCR010000000
VERSION     DAOSCR010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32282_length_2216_cov_2.880611"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1066)
                     /locus_tag="PLT69_10530"
     CDS             complement(218..1066)
                     /locus_tag="PLT69_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOJ14918.1"
                     /translation="MSLETPAFTVRYSPGLEHAAEAVMRLYPAAANQVSSRSGIAVDF
                     RPTIVVVADREVFERATSSGRTVAYAVPASSLVVIDYPRASADPFGTKGILVHELCHL
                     ALHSRIPGIPRWLDEGVAMWASEGVGELLGGAKRRPLSWLAVSGALPRLQSLESSFPG
                     DEDGLALAYEMSRSFVDFLAAGHGKDSIPNLLSALGSGMGLEEGFRVSFGAGVHELEE
                     RWRQSLATPSAIAGFLLKDTAAILLSVSAIVTVLAFVRYRSRRKALGEMAEDEADKEV
                     YGHRGL"
     gene            1407..>2216
                     /locus_tag="PLT69_10535"
     CDS             1407..>2216
                     /locus_tag="PLT69_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14919.1"
                     /translation="MKEGTKTLFVRPLLFQENITAVVLIPTLLFFFLKTAQGIHDNLG
                     LAVTVAALAGIVGLVMGAAAKYVLVKPAVDLMNKEHWEDEEVAAAVRSTSILPLVEAV
                     VVFVRWAPSAIPICIGTFYMKGLISGYEAVFGTIALLMTALLVVPFFYLASENSLLPF
                     YRRCSMKGILDSSHRPFSLSTSQKLFATMVLTALPPLGIIIGTICLSIMTGLDLSEIQ
                     LGFYIVLFEALVLTLINGYLLARGIRMSVGNMSVMLQDIAHGQGDLTKRLQI"
BASE COUNT          400 a          711 c          717 g          388 t
ORIGIN      
        1 gatgacgtgg tcgtggccgt ggacctggac gtgatcgacc tggctcgagc caggcgtgag
       61 ctcgggtaag agactccccc ggctgttcgt tccgcccgga gacgggccgg ccccgaaggg
      121 ggcggacccc gatcggcccg ggcaggaggc ccggcggcta aggagcgaag gggtcgacgg
      181 acgggtcgag cccgggcgtc cggccgactc tcagcggcta caggccgcgg tgcccgtata
      241 cctctttgtc agcctcgtcc tcggccatct ccccgagcgc cttccttctc gaccggtagc
      301 gcacgaaggc caagacggtc acgatggccg agacggacaa caggatcgcg gcggtatcct
      361 tcaagaggaa cccggctata gccgaaggcg tggccaggct ctgcctccac ctttcctcga
      421 gctcgtgcac ccccgcgccg aaggagaccc tgaaaccctc ttccaggccc atcccggagc
      481 cgagggctga aagcaggttg gggatgctgt cctttccgtg gcctgccgca aggaaatcga
      541 cgaaactcct gctcatttcg taggcgaggg cgaggccgtc ctcgtcgccc gggaacgacg
      601 actcgagtga ttgaagcctc ggcagagccc cgcttaccgc gagccaggag agcgggcgtc
      661 tcttggcgcc gcccaggagc tcacccaccc cctcgctcgc ccacatggcg accccctcgt
      721 cgagccacct cgggatgccc gggatgcggc tgtggagggc gaggtggcag agttcgtgga
      781 cgaggatccc tttcgtgccg aaagggtctg cgctcgccct cgggtagtcg atcaccacga
      841 gcgagcttgc cggaacggca taggcgacgg tgcggccgct cgaggtcgcg cgttcgaaga
      901 cctccctgtc ggcgacgacg acgatggtgg gcctgaagtc cacggcgatg cccgaccgcg
      961 aagaaacctg gttcgcagcc gcggggtaga ggcgcataac cgcctccgca gcatgctcga
     1021 gacccgggct gtacctcacc gtgaaggcgg gggtctcgag gctcatcagt ccacgaccgt
     1081 aagcgaaaag ctcaagggga aggcatgaaa gcgtgaaagc gaggagtgcg catgcgggca
     1141 cgaacgcccg cgagggggcc ggcggcgcgt tcaccgtgtc gtctgtacct tcagcgatgc
     1201 cgggcccctt catcgatcac cccttggata actgctcgag accatcttgg accaggcatc
     1261 cccgcgcgtc aaggccgacc ccccaaggtg cgccccatga ccgcgaaaaa accataaaga
     1321 aaagggggtt gggagccgat aaggagcaca acctcacggt tgtcgatcga gacgacctgt
     1381 accggggaaa cgtttggggg gcatcgatga aagagggtac gaagacgctg ttcgtcaggc
     1441 cgctcttgtt ccaggagaac atcaccgcgg tcgttctcat accgaccctg ctgttcttct
     1501 tcttgaagac cgcgcagggg atccatgaca accttggcct tgcggtcacg gtcgcggcgc
     1561 tggcgggtat cgtcggcctc gtgatggggg cggctgcgaa gtacgtgctg gtgaagccgg
     1621 ccgtggatct catgaacaag gagcactggg aggacgagga ggtcgccgcg gccgtacgat
     1681 ccacgtcgat actccccctc gtggaggcgg tggtcgtgtt cgtgaggtgg gcgccgtctg
     1741 cgatccccat ctgtatcggc acgttctaca tgaaggggct catctccggt tacgaggccg
     1801 tgttcgggac catcgccctg ctcatgaccg cgctcttggt ggtgccgttc ttctacctcg
     1861 catcagagaa cagcctcctc ccgttctaca ggcgctgcag catgaagggc atcctggatt
     1921 cgagtcaccg gcctttcagc ctgagcacct cccagaagct tttcgcaacc atggtcctga
     1981 ccgcactgcc gcccttgggt atcatcatcg gcaccatctg cctctcgatc atgaccggcc
     2041 tcgatctttc cgagatccag ctcgggttct acatcgtcct gttcgaggcg ctggtgctca
     2101 ccctgatcaa cggttacctc ctcgcacggg gcatccggat gtcggtgggg aacatgtcgg
     2161 tcatgctgca ggacatagcc catggacagg gcgatctcac caagaggctc cagatc
//
LOCUS       DAOSCR010000476         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32455_length_2208_cov_2.987459, whole genome shotgun sequence.
ACCESSION   DAOSCR010000476 DAOSCR010000000
VERSION     DAOSCR010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32455_length_2208_cov_2.987459"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLT69_10540"
     CDS             complement(<1..519)
                     /locus_tag="PLT69_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ14920.1"
                     /translation="MTKAKDRKFNEILATALELFAHYGYKKTTLKDVADKLGMTKSNL
                     YFYVSNKRDLYEKTVSMAFKGWQKAVRDAVEQAQDAGDKFSIMATQSIQYLMEHDELR
                     SIFIRDPGIFTPFPNEDRFYKINLGAIRLIEEILTLGIKQGRFHEVDVKPTAELFFSI
                     YVMFLIKTYVKSE"
     gene            748..1329
                     /locus_tag="PLT69_10545"
     CDS             748..1329
                     /locus_tag="PLT69_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14921.1"
                     /translation="MIINRLLPALAAALFAVLATGCSTVNIDWDMANSINTIEAYEEF
                     LAKHPDSVYTDEALRRVEPMRFKKAMEDGSPRALTAYLVRYPDGRHVAAVRARLKELR
                     CADTGLKKKDYPSWLRVAPAKDELHKSSWYLDKSYFGINPASIGRGFRASCDDPQAPL
                     DLAWDKDRLVYFGGKGVIVGPDGTAVLVGYDCR"
BASE COUNT          454 a          681 c          617 g          456 t
ORIGIN      
        1 ttctgatttc acataagtct tgataagaaa catgacataa atggagaaga agagttctgc
       61 tgtgggcttc acgtccactt cgtgaaaccg accttgcttg atacccaatg tcaggatttc
      121 ctcgataagc cttatggctc cgagattgat cttgtagaac ctgtcctcgt ttgggaaagg
      181 cgtgaagata cctggatctc tgatgaagat ggagcgcagc tcatcatgtt ccatcaggta
      241 ttggatggat tgtgttgcca tgatcgagaa tttgtcacct gcatcttgag cctgttcaac
      301 cgcgtctctt acagccttct gccatccctt gaaggccatg ctcaccgtct tctcgtagag
      361 atcacgcttg ttgcttacat agaagtagag gttgctcttc gtcatgccga gcttatcggc
      421 cacgtctttc agcgtggtct tcttataccc ataatgggcg aagagctcta acgcggtcgc
      481 tagtatctca ttgaatttcc ggtctttagc ctttgtcata gttgaacttt ataacatttg
      541 gttcaattag tcaacatcca ggtttcaaga catcttcaac tgcatgaact acgcaccgtc
      601 tttcgcattg cactggatct gatcaggaag cttctggtct gacacttggg tgccgccggt
      661 cgttctttga ccttggcaag aggcacaatg tgtcttatag taagatattg gcaagcgaac
      721 gtgtaccgta gacggggatt taccaccatg atcataaatc gactcctacc tgctctcgcg
      781 gccgcccttt tcgccgtcct ggcaacgggg tgctccaccg tgaacatcga ctgggacatg
      841 gcgaactcga tcaacacgat agaggcctac gaggagttcc tcgccaagca cccggacagc
      901 gtctacaccg acgaggccct caggcgggtc gagcccatgc gtttcaagaa ggccatggag
      961 gacgggtcgc ccagggccct gaccgcatac ctcgtccgct atcctgacgg ccgccacgtg
     1021 gctgcagtgc gtgcgaggct caaggaactc aggtgcgccg acactggcct caaaaagaag
     1081 gactacccgt cctggctgag ggtggccccg gcaaaggacg agctccacaa gagcagctgg
     1141 tacctggaca agtcttactt cggcatcaac cccgcgagca tcggcagggg cttcagggcc
     1201 tcctgcgacg acccccaggc cccgctcgac ctcgcctggg acaaggaccg tctcgtctac
     1261 ttcggcggca agggcgtgat cgtgggcccg gacggcaccg cggtcctcgt gggatacgac
     1321 tgcaggtgag acccggtcct tcatcgcccc cacgatatgg tgagtgagct gcaggggcca
     1381 ccaatctgca tcggcgcggg agaccgcagg cactgcatcc tccctcaaga cggcgagtga
     1441 gctgcagggt cgaccaaggt acgccgccag ccggaaagag cgcatcttcc tcgagcgccc
     1501 ggtcaccatc cacagcccgg tggcgagttg tcccagtacg accctggcag ggaggaaggc
     1561 ctgctcgagc ctgctcctgc cacccgttca ggacaggctc gtcggtcgag aaggggcacc
     1621 aggcaacacc tcttagtcga gcccgttgcg caagtctttg aggtgggccc cgcaccctgt
     1681 acgggggaga agccgaacag gaagtcaccg tcgggcctct cccataagct ggatctaagg
     1741 ccgcccgtct ctttgcatgg ggctgtcgtc tgcctgcgtt cgacgtcggg cgctttcccc
     1801 tcccggatct ccagcgcccc cccattgctt tgtcgacgcc gatgcagacg ccccaaaacg
     1861 gggaagcgtg cagggtggcc agggcacccc ccacggccat gccctgctcc tccttgaggg
     1921 ggaacaggcc tgcgttcatg tgaggcacgc ccgacgatca cccttgccgc ccgggtttcc
     1981 accacgaccc gccgccggga aagatacacg gatcctcgca ggcagaggat acgggcaccg
     2041 cgccgggttt gacaagccag ccggccgggg ataagattcc ccctcgacat ctcataccct
     2101 gagggaggcc ccatgaacct tgtctacgaa aagacgggac acattgcgaa ggtcggtctg
     2161 aacaggccca agcagctcaa cgccctcgac cccggcatcc tcctcgag
//
LOCUS       DAOSCR010000477         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32480_length_2207_cov_2.831320, whole genome shotgun sequence.
ACCESSION   DAOSCR010000477 DAOSCR010000000
VERSION     DAOSCR010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32480_length_2207_cov_2.831320"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="pheA"
                     /locus_tag="PLT69_10550"
     CDS             <1..611
                     /gene="pheA"
                     /locus_tag="PLT69_10550"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOJ14922.1"
                     /translation="TRLSVIHEFLLPIRNCLLASIPKEKIRRVFSHPQAIAQCRTWLS
                     SNLPGASIIETASTSEAAMAARSHDDAAAVASPMAAEIYGLSIVADNINDLSENITRF
                     WVISKAALPVAGHAKTSVIVTLENKPGALYGALGVFARSSINLTKIESRPSKKNPWEY
                     IFFIDFQGNLADTNVAEAMEELRSATRDLIVLGSYPEGRTLT"
     gene            608..1696
                     /gene="hisC"
                     /locus_tag="PLT69_10555"
     CDS             608..1696
                     /gene="hisC"
                     /locus_tag="PLT69_10555"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOJ14923.1"
                     /translation="MKVPEPCPWVMGIPAYVPGRSKEEIARAYGVSSPIKLASNENPL
                     GPSPMAVAAMREALTCCHLYPDPQARELCEAAAEYLGCSPDSVVAGNGSDEIIDLVCR
                     AFLKPGDAVVIPGCTFSYYTIASRACGAVVRKAPMRGNGIDPEATADLAVPGTKMVFV
                     ANPNNPTGSCLMKHEILRLIERVPPDCVVLVDEAYAAFVRREGFSSSVGMIEAHPNVV
                     CVHTLSKSHGLAGVRVGFAVASPALREHLLRIKPPFNVNVLAQKAGAAALKDREFLAR
                     TLETTWNALDRLEDAFTRLGLPYEPSQTNFVMVHIGKDAGRVYEALLSRGIITRHMAG
                     FGLPEHLRVSAGTPGEIDAFIEALGEVL"
     gene            1693..>2207
                     /locus_tag="PLT69_10560"
     CDS             1693..>2207
                     /locus_tag="PLT69_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOJ14924.1"
                     /translation="MRVAVIGLGLIGASLARALAGKAEVLGMDANPSVVRRAEEEGVV
                     LRGCADLRDLAGCDMVVLALPVGSIVDAARKVLPFVGERTTITDTGSTKARIVGEMDK
                     ITGSFVGSHPIAGKERPGYDASDRDLFVGAAAIITPSETTSPDRIAEVKRLWEACGST
                     THIMDPASHDRL"
BASE COUNT          386 a          744 c          739 g          338 t
ORIGIN      
        1 ccacgcgcct gtccgtcatc cacgagttct tgctgcccat caggaactgc ctgctcgcct
       61 ccatccccaa ggagaagatc cggagggtct tctcccaccc gcaggccata gcgcagtgca
      121 ggacctggct gtcctcgaac ctgcccggcg cctccatcat cgagaccgcg agcacctcag
      181 aggccgccat ggcggcccgc tcccacgacg acgcggccgc cgtggcgtcc cccatggctg
      241 cggagatcta cggcctgtcg atcgtcgcgg acaacatcaa cgacctcagc gagaacatca
      301 cgaggttctg ggtgatctcg aaggccgccc tgcccgtggc gggccacgcg aagacctcgg
      361 tgatcgtgac cctcgagaac aagcccgggg ccctctacgg ggcgctcggc gtgttcgccc
      421 gctcgtcgat aaacctgacg aagatagaat cgaggccgtc caagaagaat ccctgggaat
      481 acatcttctt catcgacttc caggggaacc ttgcggacac aaacgtggcc gaggccatgg
      541 aagagctcag gtccgcaacc agggacctga tcgtactcgg ctcctaccct gaggggagga
      601 ccctcacgtg aaggtgcccg agccctgccc ctgggtcatg gggatccccg cctacgtgcc
      661 ggggagatcc aaggaggaga tcgcccgggc ctacggggtg agctctccca tcaagctcgc
      721 ctcgaacgag aaccccctcg ggccgtcgcc catggcagtg gccgcgatgc gggaggcgct
      781 cacgtgctgc cacctctacc ccgaccccca ggcgcgcgag ctgtgcgagg ctgcggcgga
      841 atacctcggg tgcagcccgg acagcgtggt cgccggcaac ggctcggacg agatcatcga
      901 cctcgtgtgc agggcctttt tgaagccggg cgacgccgtc gtgatccccg ggtgcacctt
      961 cagctactac acgatcgcat ccagggcgtg cggggccgtg gtgaggaagg cgcccatgcg
     1021 cggcaacggc atagaccccg aggccacagc cgatctcgcg gtgccgggca cgaagatggt
     1081 cttcgtggcc aaccccaaca accccacggg aagctgcctc atgaaacacg agatcctccg
     1141 gctcatagag agggtcccgc ccgactgcgt cgtcctcgtg gacgaggcgt acgcggcatt
     1201 cgtgcgcagg gagggcttct cgagctcggt ggggatgatc gaggctcacc cgaacgtggt
     1261 gtgcgtgcac accctgtcga agtcgcacgg gctggcaggc gtgcgcgtgg ggttcgcggt
     1321 cgcctcgccc gcgctcaggg agcacctgct ccgcatcaag ccgccgttca acgtgaacgt
     1381 gctggcgcag aaggcggggg cggcggcgct taaggaccgg gagttcctcg ccaggaccct
     1441 cgagaccacg tggaacgcgc tggacaggct cgaggacgcc ttcacgaggc tcggcctccc
     1501 gtacgagccc tcacagacga acttcgtcat ggtgcacatc ggcaaggacg cagggcgcgt
     1561 ctacgaggcc ctgctctctc gggggatcat cacgaggcac atggcgggct tcggcctgcc
     1621 ggagcacctg agggtgagcg cgggcacgcc cggagagatc gacgccttca tcgaggccct
     1681 gggcgaggtg ttatgagggt cgctgtcatc ggtctcggtc tcatcggcgc ctcccttgcc
     1741 agggccttgg ccggcaaggc cgaggtcctg ggcatggacg ccaacccctc ggtcgtgagg
     1801 cgcgcggagg aggaaggggt tgtcttgcga ggctgcgccg acctgcggga cctggccggg
     1861 tgcgacatgg tcgtgctcgc cttgcccgtt gggagcatcg tggacgcggc aaggaaggtg
     1921 ctcccgtttg tgggcgagcg gacgaccatc accgacaccg gctccaccaa ggcgcgcatc
     1981 gtcggcgaga tggacaagat cacgggctcg ttcgtgggtt cgcacccgat agcgggcaag
     2041 gagaggcccg gctacgatgc gagcgacagg gacctgttcg tgggggcggc ggccatcatc
     2101 accccttccg agaccaccag ccccgaccgc atcgccgagg tcaagaggct ctgggaggct
     2161 tgcggatcca ccacccacat catggacccc gcatcccacg acaggct
//
LOCUS       DAOSCR010000478         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32598_length_2201_cov_3.239981, whole genome shotgun sequence.
ACCESSION   DAOSCR010000478 DAOSCR010000000
VERSION     DAOSCR010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32598_length_2201_cov_3.239981"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /gene="tssF"
                     /locus_tag="PLT69_10565"
     CDS             complement(<1..1315)
                     /gene="tssF"
                     /locus_tag="PLT69_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HOJ14925.1"
                     /translation="MDDTLLRYYERELSFLREMGAQFARKYPKIAGRLLLEPDRCEDP
                     HTERLIEAFALIAGRIHRKIDDGFPEITESLFHILHPHYINPIPSMTVVRFDPVKQTV
                     GPPGFVVEKGTQLYSKPVGGAACQFAVAFPVTILPVEVRQAGVQEPRRMAQGARQDIS
                     IGLETLNAIDVSRIQADKLRFYLNGSHQSVFALYELLFNHVTALEIHGLGSGGSPAAF
                     QLPPGSLKQVGFEDDEALIPATPRSFPGYLRLLEYFTFPEKYLFFDIAGLSSALQGLH
                     GTAFEIRISIDRPVPPGIVIDADTFCLNAAPAVNLFRKTAEPIRLDRRSTEYRVIPDL
                     RRQDATEVYHVERVVSAAATDPSRTLEIRPLYSLSHHEAHEGPQVFWHMRRVPSPRKG
                     DNGTDVYLSFTDAALSPADPGVETVTVSTVCTNRDLPGRLPFGDPS"
     gene            complement(1322..1795)
                     /gene="tssE"
                     /locus_tag="PLT69_10570"
     CDS             complement(1322..1795)
                     /gene="tssE"
                     /locus_tag="PLT69_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HOJ14926.1"
                     /translation="MRPSEENLLASVLDRLVDEEPEVSFEPVPRRLLDMRRVRELVVR
                     DLENLLNTRCTVEFPDASFKEVGRSVFVYGIRDYTSLSPRSAAVRKSLRQDIERAIAV
                     FEPRLRNVKVHLEAEQKGHSLRFRITALIVVEPEKEPVQFDTLFDINRSQYTISR"
BASE COUNT          342 a          716 c          750 g          393 t
ORIGIN      
        1 ccgaggggtc gccgaagggc agcctgccgg gcaggtcgcg gttggtgcac accgtcgaga
       61 cggtgacggt ctcgacacca gggtccgcgg gggacagggc ggcgtccgtg aagctcaggt
      121 acacgtcggt cccgttgtcg ccctttcgcg gggacggcac cctcctcatg tgccagaaca
      181 cctgcggccc ctcgtgcgcc tcgtggtggc tcaggctgta gagggggcgg atctcgaggg
      241 tccgggaagg gtcggtggcg gccgcgctca ccacccgctc gacgtggtat acctcggtcg
      301 cgtcctgcct tcgaaggtca gggatgaccc tgtactcggt ggacctcctg tccagccgga
      361 tcggctccgc ggtcttcctg aagaggttca cggcgggcgc cgcgttgaga cagaaggtgt
      421 cggcgtctat gacgatgccg ggcggcaccg gcctgtcgat ggatatcctg atctcgaagg
      481 cggttccgtg gaggccctgg agcgcggacg aaagccccgc gatatcgaag aacaggtact
      541 tctcggggaa ggtgaagtac tcgaggagcc tcaggtagcc ggggaacgac ctcggggtcg
      601 cagggatgag ggcctcgtcg tcctcgaacc ccacctgctt caggctcccg ggggggagct
      661 ggaaagcggc gggggacccc ccgctcccga ggccatggat ctccagggcc gtgacgtggt
      721 tgaacaggag ctcgtacagg gcgaacacgc tctggtggga cccgttcagg tagaacctga
      781 gcttgtccgc ctggatcctc gagacgtcga tggcgttgag ggtctcgagc ccgatggaga
      841 tgtcctgcct ggcgccctgc gccatgcgcc tgggttcctg gaccccagcc tgcctgacct
      901 cgacgggcag gatcgtgacg ggaaaggcca ccgcgaactg gcacgccgct cctcccacag
      961 gcttggagta gagctgcgtc cccttctcca cgacgaaccc cggaggcccc acggtctgct
     1021 tgacggggtc gaacctcacc accgtcatgg acgggatggg gttgatgtag tggggatgga
     1081 ggatgtggaa gagggattcc gtgatctcgg ggaaaccgtc gtcgattttc cggtgtatgc
     1141 ggccggcgat gagcgcgaac gcctcgatga gcctctcggt gtgcgggtcc tcgcacctgt
     1201 ccggctcgag cagcaacctc cccgcgatct tcgggtactt ccgggcgaac tgcgcgccca
     1261 tctcgcggag gaagctcagt tcacgctcgt agtagcgaag cagggtgtcg tccacggcgc
     1321 gtcacctcga gatggtatac tggctcctgt tgatgtcgaa gagcgtgtcg aactgcacgg
     1381 gctccttttc cggctccacc acgatgagcg ccgttatcct gaacctgagg ctgtggccct
     1441 tctgctcggc ctccaggtgg accttcacgt ttctgagcct gggctcgaac acggcgatgg
     1501 cccgctcgat gtcctgcctg agggacttgc ggacggcggc gctcctggga ctgagcgagg
     1561 tgtaatccct gatcccgtac acgaacacgc tgcggccgac ctccttgaag gaggcgtccg
     1621 ggaactccac ggtgcacctc gtgttgagga ggttttccag gtcccgcacg accagctccc
     1681 tgaccctgcg catgtcgagc agtcgcctgg gcacaggctc gaaagacacc tccggctctt
     1741 catccaccag cctgtcgagc accgaggcga ggaggttctc ttcagagggc cgcacaacgc
     1801 tcctttcgaa ctcggcggat accccgcgcc cgatgggctc cctcggtccg gatgccgggt
     1861 accgcctggg ggctcgatcc cgttctcgga aaggacctca tgggagacct ggaggccggc
     1921 ttttcgccgg ggtaccggcc tccatgccca ccggtttccc ggagacggcg gcgagccgac
     1981 cggttcgaac gcacccgccg gcctccggcc tgtcccctgg aagccctccc gcccatgtca
     2041 gcggtgcccc gtctccacca gggagagggc cccgggggcg gagaggatca cccgaaggac
     2101 atcccgcgac ctctgaggga ttccccgtca cgcgcccttg ttcgcgatga ggtcccactg
     2161 ggccttgaca tcgcccttgg gcttgccggt cgcgtggtcg g
//
LOCUS       DAOSCR010000479         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32604_length_2201_cov_2.744641, whole genome shotgun sequence.
ACCESSION   DAOSCR010000479 DAOSCR010000000
VERSION     DAOSCR010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32604_length_2201_cov_2.744641"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLT69_10575"
     CDS             complement(<1..792)
                     /locus_tag="PLT69_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ14927.1"
                     /translation="MHYLGIDAGSLYLKFWLENEMGEVRVAQSVHHRGLPQETALGML
                     SRMHPKPDCVYASGLGMDHGSITSITDGLLAEVACMRDAYPQRTMLVFGAEKIEFVRF
                     DDDGGILEYRTNTSCAAGTGSFLDEQMVRLGLDPEALETIPLIENAPLVATRCAVFAK
                     TDLVHLQQEGYSAHAMYNGLCRGLAASSLKSVFGASLPEGEGMIATGGLAANPHFRHF
                     LLSLCPGLSIADTPAFTRARGVCAQARPHPRVRSGPLPSPAGGPAR"
     gene            complement(975..1550)
                     /locus_tag="PLT69_10580"
     CDS             complement(975..1550)
                     /locus_tag="PLT69_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOJ14928.1"
                     /translation="MSIATIVFIALALAMDACAVAISYGVAMKELRVRAAFRIAFAFG
                     SFQAFMPVVGYMAGLGVRRFMQDYDHWVAFGLLAVIGIKMISESFSLDGAKRSTDADH
                     IPTLLLLSVATSIDALAVGMSLSLLKVDIIQPALVIGAVTFAVSYLGTVLGTMVGHLF
                     ERKMEFAGGLILIAIGLKILYEHLFSQRPLP"
     gene            2093..>2201
                     /locus_tag="PLT69_10585"
     CDS             2093..>2201
                     /locus_tag="PLT69_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ14929.1"
                     /translation="MKIKPLHDRVIVKRIDEEQKTKGGIIIPDTAKEKPQ"
BASE COUNT          422 a          676 c          742 g          361 t
ORIGIN      
        1 gcgagcgggg cctccagccg gtgaaggcag tgggccgctt ctgactcgag gatgcggccg
       61 ggcctgtgcg cacacacccc tcgccctcgt gaatgcgggt gtgtcggcga tggaaaggcc
      121 ggggcacagg gacaggagga agtgccggaa gtgggggttc gccgcgaggc cgccggtcgc
      181 tatcatgcct tcgccctcgg ggaggctggc gccgaacacg ctcttcaggc tcgatgcggc
      241 cagccccctg cacaggccgt tgtacatcgc gtgcgccgag tacccttcct gctggaggtg
      301 gacgaggtcg gtcttcgcga acaccgcgca acgggtcgca accaggggtg cgttctcgat
      361 gagggggatg gtttccagcg cctcagggtc gagaccgagc ctcaccatct gctcgtccag
      421 gaacgaaccc gtaccggccg cgcacgaggt gttcgtgcgg tattcgagga tgcccccgtc
      481 gtcgtcgaag cggacgaact cgatcttctc cgcgccgaac acgagcatgg tgcgctgggg
      541 gtaggcgtcc ctcatgcagg caacctcggc gagcaggccg tcggtgatcg acgttatcga
      601 cccgtggtcc atgcccaggc cggacgcata cacgcaatcg ggcttcgggt gcatgcgtga
      661 aagcatgccc agcgccgtct cctggggcag gccccggtgg tggaccgatt gcgcgacgcg
      721 cacctcgccc atctcgttct cgagccagaa cttgagatac aggctccccg cgtcgatgcc
      781 gaggtaatgc atgtctttcc tcccataccc gcccgccctg caggcaatga cattgcaacc
      841 ctctcaagat accacagctc gttctcccca tcaaggccgg cctccgaaga cagggcacga
      901 ggccttgcga ggaatcgagc ttcgtcctcg gatgggcgcg cgcctgcgcg gtttcgccgc
      961 ctccccggcc cgggtcaggg caaagggcgc tggctgaaga ggtgctcgta gaggatcttg
     1021 agcccgatgg cgatgaggat gaggccgcct gcgaactcca tcttcctctc gaagaggtga
     1081 cccaccatgg tccccaggac ggtgcccagg tacgacacgg cgaaggtgac cgcgccgatg
     1141 acgagggcag gctggatgat gtccaccttg aggagggaga ggctcatccc cacggccagc
     1201 gcatcgatgc tggtcgccac cgagaggagg agcagggtgg ggatgtggtc cgcgtcggtg
     1261 ctgcgcttgg cgccgtcgag ggaaaaggac tcggagatca tctttatgcc gatgacggcc
     1321 aggagcccga acgcgaccca gtggtcgtag tcctgcatga agcgcctgac gccgagacct
     1381 gccatgtagc cgacgacggg catgaacgcc tggaaagacc cgaaggcgaa cgctatgcgg
     1441 aaggccgcgc ggactctgag ctccttcatg gccacaccgt acgagatggc gacggcgcag
     1501 gcgtccatgg cgagggccag cgcgatgaag acgatggttg ctatgctcac ggcgaactcc
     1561 tcctcggccc ttgaccacag gcccgccccg gttctcttgc caggaccacc tgggcattgt
     1621 gcaggtcaaa gcggtgaaag tcaaaccgag gatgcccccg cagggcgccg atcgcctggc
     1681 atgcaccgtt actcctccga aagacgcggc cgtcctgcga ttccgtcgag cgatgcggca
     1741 ggcggcgtga tgccttcctg cgctcgcgcc gaggccggga tccatgcccc tggcgggctc
     1801 ccttgggccg ggccaggcga acgccgcatg aggggtgcgc ctgaggcgcc tggagtcgac
     1861 gggaaggacc ggctcgagct tgtctgtagc tggcatatca aagatatcag gagatttcgg
     1921 gaggtatcgt cagtgaaaag atgatatcga agccgaggca gtgacccggg gtgattttcg
     1981 aatggcaagg cctgatgggg ggattgtgat cccgtgcgca tcttggtaca tgatcagcac
     2041 tctcataaag tgagtgctaa taccgcgcaa gacatccgcc aggaggaaga ggatgaagat
     2101 caaaccgttg cacgacaggg tcatcgtcaa gaggatcgat gaggaacaga agaccaaggg
     2161 cggcatcatc atccctgaca ccgccaagga gaagccccag a
//
LOCUS       DAOSCR010000480         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32642_length_2199_cov_2.639459, whole genome shotgun sequence.
ACCESSION   DAOSCR010000480 DAOSCR010000000
VERSION     DAOSCR010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32642_length_2199_cov_2.639459"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="lpoB"
                     /locus_tag="PLT69_10590"
     CDS             <1..447
                     /gene="lpoB"
                     /locus_tag="PLT69_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOJ14930.1"
                     /translation="EKMVNSLLASPVVAGGRRPVFYIQTVRNRTDEHIDTRAVTDKIR
                     VTLLKSGKVRFTAVSDVKDEIVNQLEFQTSSGMVDPATAKAMGRLVGADYFLYGELAS
                     IRKSAGRVKDVYYKFTLNLVNISNGLIEWADEKEIRKQAKKPLFGN"
     gene            692..1249
                     /locus_tag="PLT69_10595"
     CDS             692..1249
                     /locus_tag="PLT69_10595"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOJ14931.1"
                     /translation="MNLDDLKHKWEKVNARTAVLYGAIVLLVVFSNPRWWSCLLGIFP
                     VIAGEALRVWATGHLRKNEELTTSGPYAHLQSPLYLGSYLIGTGLCIMALNPVIWVLG
                     SLVFFLSYIPRKQETEWGRLERKFGEEFLKYKREVNYFFPRIDPYPEGSKHLWSFAQV
                     IENTEHQTALAVGVIALFIIAKLAW"
     gene            complement(1455..>2199)
                     /gene="ilvE"
                     /locus_tag="PLT69_10600"
     CDS             complement(1455..>2199)
                     /gene="ilvE"
                     /locus_tag="PLT69_10600"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOJ14932.1"
                     /translation="RLFDSAKAYLMTIPFTEGDIMDAAKQTIKANGLTEAYIRPIAFI
                     GDGVMGVHPQNNPVRVAVICWEWGAYLGDDGLENGVRVKTSSFSRHHPNIMLTKAKVT
                     GNYVNSILAKIEVTRLGYEEALMLDTQGYVAEGSGENVFSVKDNVLYTPPVTSILPGI
                     TRASVITLAKELGYEVREQMITRDELYIADEVFFTGTAAEITPAREIDDRTVGTGRPG
                     PVTKALQKAYFDTIRGKNPAHLDWLDYID"
BASE COUNT          380 a          673 c          733 g          413 t
ORIGIN      
        1 gagaagatgg tcaattccct gctcgcgagc cccgtggtgg caggcggcag aaggcccgtt
       61 ttctacatcc agaccgtgag gaacaggacc gacgagcaca tagacacgag ggcggtcacg
      121 gacaagatac gggtcacgtt gctcaagtcc ggcaaggtgc gcttcacggc ggtctccgac
      181 gtgaaggacg agatcgtgaa ccagctggag ttccagacga gctctggcat ggtggacccc
      241 gcgaccgcca aggcgatggg caggctcgtg ggagcggact acttcctcta cggggaactg
      301 gcgtcgatcc gcaagtccgc cggccgcgtg aaggacgtgt attacaagtt cacgctcaac
      361 ctcgtgaaca tatcgaacgg cctcatcgag tgggcggacg agaaggagat caggaagcag
      421 gccaagaagc ccttgttcgg caactgatcc cgatgcacgc gaggcggggt gcggcttttg
      481 ctcttgccgg gcccaacagc ggccatggcg tcttcgcgag gccgcagcag gggcctggtg
      541 aggtcccggc aaccaccggt gcgccgcggg atgcctcccg ccggaggcgg cccggccgca
      601 caccggcttc aggttgggct tgactggccg gcgacgaacc ggatacaaac gtgtgcgatc
      661 ccggcacggg gtcgacgggg gagggcctgg tatgaacctt gacgatctca agcacaagtg
      721 ggagaaggtg aacgcgagga ccgcggtcct ctacggtgcc atcgtcctgc tcgtggtctt
      781 ttccaaccca aggtggtggt cctgcctctt gggcatcttc cccgtgatcg cgggcgaggc
      841 cctcagggtg tgggcgacgg gccacctgcg caagaacgag gaactcacca cctcggggcc
      901 ctacgcgcac ctccagtccc ccctctacct cgggagctac ctgatcggca ccgggttgtg
      961 catcatggcc ttgaacccgg tgatctgggt gctgggcagc ctcgtcttct tcctgtccta
     1021 catcccccgc aagcaggaga cggagtgggg gcgcctcgag cgcaagttcg gagaggagtt
     1081 cttgaagtac aagcgggagg tcaactactt cttccccagg atcgatccct acccggaggg
     1141 gagcaagcac ctgtggtcgt tcgcccaggt gatcgagaac accgagcacc agaccgcgct
     1201 ggccgtgggc gtcatcgccc tgttcatcat cgcgaagctc gcgtggtagc ctttctttga
     1261 atcaaaccgc aggtggtgcc taccggccca tgctcgggag ggtgtctcca tgcatggggc
     1321 agcgtcaatc ggggccgccc accgtgcatg gacccccagg gcatacccac cgggtcccgg
     1381 ccccggcgag aaggaaaccc ccgggagatg cggcccgggg gttcgttctt tcccgctgcg
     1441 tctgcagggg aagctcagtc gatgtagtcg agccagtcga gatgggcggg gttcttgccc
     1501 cgtatggtat cgaagtacgc cttctggagc gccttggtca cagggccggg tctgcccgtt
     1561 cctacggtgc ggtcgtctat ctccctggcg ggagtgatct cggccgccgt cccggtgaaa
     1621 aagacctcgt cggcgatgta gagctcgtcc ctggtgatca tctgttccct gacctcgtag
     1681 cccagttcct tggcgagggt gatgaccgag gcgcgggtta tgcccggcag gatgcttgtg
     1741 acgggcgggg tgtacaggac gttgtccttc accgagaaca cgttttcccc gctgccctcc
     1801 gccacgtaac cctgggtgtc gagcatgagg gcctcctcgt agccgagcct cgtgacctcg
     1861 atctttgcga ggatggaatt cacgtagtta cccgtcacct tcgccttggt gagcatgatg
     1921 ttcgggtggt gcctggagaa cgaggaggtc ttcacgcgca cgccgttttc gagcccgtcg
     1981 tcgccgaggt aggctcccca ttcccagcag atgacggcga cccggacggg gttgttctgg
     2041 gggtgcacgc ccatgacacc gtcaccgatg aacgcgatgg ggcgtatgta cgcctcggtg
     2101 aggccgttgg ccttgatggt ctgcttcgcc gcgtccatga tgtcgccctc ggtgaacggg
     2161 atcgtcatga ggtacgcctt ggccgagtcg aagagcctc
//
LOCUS       DAOSCR010000481         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32697_length_2196_cov_2.704344, whole genome shotgun sequence.
ACCESSION   DAOSCR010000481 DAOSCR010000000
VERSION     DAOSCR010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32697_length_2196_cov_2.704344"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLT69_10605"
     CDS             complement(<1..606)
                     /locus_tag="PLT69_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HOJ14933.1"
                     /translation="MNFLSTMRGMALKRAESLVQPEGRADAVRPFGQALRGRGISVIA
                     EVKYATPTDGPLGLPQALAAKFERLGAAAVSCLTEPEFFHGSLEHLGRIRRACSLPVL
                     MKDFVVDTRQVDAGRALGADAVLLITEMLTEDELKTLHAHARALGMDCLVECHGPQGL
                     EKALAVGATLVGVNCRDLATLKVDRRRHEELIGLLPGDVVKV"
     gene            complement(603..1601)
                     /gene="trpD"
                     /locus_tag="PLT69_10610"
     CDS             complement(603..1601)
                     /gene="trpD"
                     /locus_tag="PLT69_10610"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019022706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOJ14934.1"
                     /translation="MKEAFEKIIAGRHLSADEMEGCFDLIMGGMATDAQIAGFLTALR
                     IKGETVEEIAAAASVMRRHATRVTTGCSTVLDTCGTGGDNASTFNISTAVAFVMAGAG
                     VKVAKHGNRSVSSSSGSADCLEALGVPIDLGPQEAARMVNETGFCFLFAPLYHPSMKH
                     AMPARRQLGIRTVFNVLGPLANPANATHQVIGVYSSSLVEPICEVLYALGLEGAMVVN
                     GGLDEVSISGPTVYARIRGSKIEKGEIRPEDAGLEPMPGHELRVGSPAESAALIEELF
                     TGKRRGACLNSLLMNAAAAFMALDPSVDVAEGVRLARGVIEGGKALEALDRARGRA"
     gene            complement(1598..2179)
                     /locus_tag="PLT69_10615"
     CDS             complement(1598..2179)
                     /locus_tag="PLT69_10615"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOJ14935.1"
                     /translation="MTLVIDNYDSFTFNLVQILAARGVEVVVRRNDEIGLSELDSMMP
                     EAVVISPGPGDPTDAGISVSAVEHLAGRVPILGVCLGHQCIAHAFGARIVHAKRVMHG
                     KLSPISHDGTGLFEGLPDNFPAVRYHSLAVDGDSLPSSLRVCARAYDDGEIMGLCNEH
                     MRLYGVQFHPESIATEFGEVIMERFIALSRRNP"
BASE COUNT          354 a          777 c          697 g          368 t
ORIGIN      
        1 gaccttcacg acgtccccgg gcaaaagccc gatgagttcc tcgtgccgcc ttcggtccac
       61 cttcagggtg gcgaggtccc ggcagttgac gcccaccagc gtcgcaccca ccgcaagcgc
      121 cttctcgaga ccctgagggc cgtggcactc gaccaggcag tccatcccga gcgccctggc
      181 gtgggcgtgc agcgtcttca gctcgtcctc ggtcagcatc tcggtgatca ggagcacggc
      241 gtccgcgccc agcgccctgc ccgcgtccac ctggcgggta tccaccacga aatccttcat
      301 gagcacgggc aggctgcagg cacgcctgat gcgcccgagg tgctcgaggc tgccgtggaa
      361 gaactcgggc tcggtgaggc acgacaccgc cgcagcgccg aggcgctcga acttcgccgc
      421 aagcgcctgg ggcaggccca ggggaccgtc ggttggggtg gcgtatttca cctccgcgat
      481 cacggagatg ccccgccccc tgagggcctg gccgaacggg cgcacggcgt ccgctcgacc
      541 ctcgggctgc acgaggctct cggccctctt cagggccatc ccgcgcatgg tggagaggaa
      601 gttcacgccc gccccctcgc cctgtccagg gcctcgagcg ccttaccgcc ctcgatcact
      661 cctctggcga gcctgacacc ctctgcgacg tccaccgacg ggtcgagggc catgaacgcc
      721 gctgccgcgt tcatgagcag gctgttcagg cacgcccccc tgcgcttccc cgtgaacagc
      781 tcctcgatga gggccgcgga ctcggcggga gagcccaccc tgagctcgtg tcccggcatg
      841 ggctcgagac cggcgtcttc gggcctgatc tcgcccttct cgatcttcga gccccgtatg
      901 cgtgcgtaca ccgtgggacc cgagatggag acctcgtcga ggccgccgtt gaccaccatc
      961 gcgccttcga ggcccagggc gtacaggacc tcgcagatgg gttccacgag ggaagaggag
     1021 tacacgccga tgacctggtg tgttgcgttc gccgggttgg caagcggccc gagcacgttg
     1081 aagaccgtgc ggatgccgag ctgcctgcgc gcaggcatgg cgtgcttcat gctcgggtgg
     1141 tacaagggcg cgaagagaaa gcagaaaccc gtctcgttca ccatgcgcgc cgcttcctgc
     1201 gggcccaggt cgatgggcac acccagggcc tcgaggcagt cggcgctgcc cgacgacgaa
     1261 ctcaccgagc ggttgccgtg cttggcgacc ttgacccccg ctccagccat gacgaaggcc
     1321 accgccgtgg agatgttgaa ggtcgacgcg ttgtcgccgc cggtcccgca ggtgtccagg
     1381 accgtcgagc accccgtcgt caccctcgtc gcgtgcctgc gcatgacgga cgccgccgcg
     1441 gctatctcct ccacggtctc ccccttgatc ctgagcgcgg tgaggaaacc ggcgatctgg
     1501 gcatcggtcg ccatgccgcc catgatcagg tcgaagcacc cctccatctc gtccgccgac
     1561 aagtgtcgtc ccgcaatgat cttctcgaac gcttccttca tggattcctc cttgagagag
     1621 cgatgaaccg ctccatgatc acctcaccga attcggtggc tatggactcg ggatggaact
     1681 ggactccgta gagcctcatg tgctcgttgc acaggcccat gatctccccg tcgtcgtagg
     1741 cccgggcgca tacccgcagg ctggaaggaa ggctgtctcc atccaccgcc agggagtggt
     1801 acctgaccgc ggggaagttg tcgggaagcc cctcgaacaa acctgtcccg tcgtgggaga
     1861 tgggggacag ctttccgtgc atgaccctct tggcatgcac gatcctcgcg ccgaacgcat
     1921 gggcgatgca ttggtggccg aggcacacgc cgaggatcgg cacgcgcccg gcgagatgct
     1981 cgacggcgct cacgctgatg cccgcatccg tcgggtcgcc gggcccgggc gagatgacga
     2041 ccgcctcggg catcatcgag tcgagctccg agaggccgat ctcgtcgttc ctcctcacga
     2101 ccacctcgac ccccctcgcg gcgaggatct ggaccaggtt gaaggtgaac gaatcgtagt
     2161 tgtctatcac cagggtcatg actcacctcc cgagcg
//
LOCUS       DAOSCR010000482         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_32902_length_2186_cov_3.770061, whole genome shotgun sequence.
ACCESSION   DAOSCR010000482 DAOSCR010000000
VERSION     DAOSCR010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32902_length_2186_cov_3.770061"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLT69_10620"
     CDS             complement(<1..221)
                     /locus_tag="PLT69_10620"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071193.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOJ14936.1"
                     /translation="MKVFLSEEEIPRQWYNLAADLPTPLLPPLGPDGNPVRPEDLSPV
                     FPMNLIEQEVSDKRWIDIPEEVLQLLYQWR"
     gene            complement(895..>1121)
                     /locus_tag="PLT69_10625"
     CDS             complement(895..>1121)
                     /locus_tag="PLT69_10625"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ14937.1"
                     /translation="THSGPMVGGKQLKILYITQVDVAPPRFQVVVNTDEAVPRTYARF
                     LANSLRKACGLEEVPVEIRFVPRRHKRRAG"
     assembly_gap    1122..1134
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1135..>2186)
                     /gene="der"
                     /locus_tag="PLT69_10630"
     CDS             complement(<1135..>2186)
                     /gene="der"
                     /locus_tag="PLT69_10630"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ14938.1"
                     /translation="KSTLFNALIGEWKSIVGPKRGITRDRIFGRWTLAAGLDVDLVDT
                     GGFDTRGDIDLSEGVLRQTMTAIEEAHLVVCLFDGSEPPTPDDRELVDLLRKKGKPAV
                     YAVNKVDDPSNDLALAAFYELGIDDPVAVSARNRLGLADLAEAVRLSLGEATPARAHE
                     GQGLRVGILGRPNVGKSLMLNRITGSERALVSPVAGTTRDSVDTNVVRDGKTYTFVDT
                     AGIRRRSRREDSIEFVSVTRALRTIERSDVCLLVLDAAEGLTDQDKRLCGAILDRGRA
                     LGVVVNKVDLVDEGGVRLIRESLQRGLSFMPGLPVLFVSALTGAGLDRVYPLADELFA
                     KATTRVPTARLNRFLK"
BASE COUNT          332 a          764 c          681 g          396 t
ORIGIN      
        1 cgccactggt agagcagctg aaggacctct tcggggatgt ctatccaccg tttgtcgctc
       61 acctcctgct ctatgaggtt catcgggaag acaggggaga ggtcttcggg tctcaccggg
      121 ttgccgtccg ggcccagggg gggcaacagc ggcgtcggca ggtcggcggc caggttgtac
      181 cattgcctgg gaatctcttc ctcgctcaga aagaccttca tgtatcatcc tccatgctgt
      241 agtttttgtg catcgccact catgcgaaga acccttttca tgaccctttc ccgctgtatg
      301 gaccaggctc gcccgctcga cccaggccgg caccgcctcg cggacgtctt gcggggtgaa
      361 aggcccggca tctcgggccc ggcgcaccgc acagatgtga tcggccggtg ctctgccggc
      421 cgggggcgca ccacgtccgc gtttctttgc atagaaacac gtaaatacct ccctgtcaag
      481 ccctcattcc cccgcacgtc cattctctgc cctctatctg cgcccaaggc cctctcgacc
      541 gggcttcggg gtttcctctc cgcacgccgc aggcccgttc atcgagggcc gtcttgcgcg
      601 cacgcctcga tgccggcacg gctgaaggtg aaggcatccc gtctcacgtc ggcaaacggg
      661 cgaaacaccc cttccggcat ggtccctcgg cgggtaggac gggaggtggc caccgcgcgt
      721 gggcccggcc cgtacaccgg atacggtcac ggcgggttct tcactctgcc gcaccgtggt
      781 ttcacctcga aacccacgca tcggccaaac cgccgatggc ggccgccgac gggtcccgta
      841 cgacggccac cccagatccc ggggggagga acgcgcggca ttcgtcgggg gcgttcatcc
      901 cgctcgtctc ttgtgcctgc gcggcacgaa gcggatctcc acgggcacct cctcgagacc
      961 gcaggccttc ctcagcgagt ttgcgagaaa cctcgcgtac gtcctgggca cggcctcgtc
     1021 cgtgttcacc acgacctgga acctgggcgg cgccacgtcg acctgggtta tgtagaggat
     1081 cttgagctgc ttgccgccca ccatcggccc ggagtgcgtg gnnnnnnnnn nnnnccttga
     1141 ggaagcggtt gagccgggca gtgggcaccc tcgtcgtcgc cttggcgaag agctcgtccg
     1201 cgaggggata gaccctgtcc agtcccgcgc ctgtgagcgc cgagacgaag agcacgggca
     1261 ggccgggcat gaaggagagc cccctttgca acgattccct gatcaggcgc accccgccct
     1321 cgtccacgag gtccaccttg ttcaccacca cgcccagggc cctgccccga tcgaggatcg
     1381 cgccgcagag gcgcttgtcc tggtccgtga ggccctccgc cgcatccagg acgagcaggc
     1441 acacgtcgga gcgctcgatc gtcctgagcg ccctcgtcac gctcacgaac tcgatggagt
     1501 cttccctgcg ggacctcctc ctgatgccgg ccgtgtccac gaaggtgtag gtcttcccgt
     1561 cccgcacgac gttcgtgtcc acgctgtcgc gggtggtgcc cgcaaccggc gagaccaagg
     1621 ccctctcgct ccccgtgatg cggttgagca tgagcgactt gcccacgttc ggcctcccga
     1681 ggatacccac gcgaagcccc tgtccctcgt gggcccttgc gggcgtcgcc tccccgaggg
     1741 agagcctcac cgcctccgcg aggtccgcca gtcccaggcg gttgcgcgcg gacacggcta
     1801 ccgggtcgtc tatgccgagc tcgtagaagg ccgcgagtgc gagatcgttc gacgggtcgt
     1861 cgaccttgtt cacggcgtag acggcgggct tgcctttctt cctgaggaga tcgacgagct
     1921 ccctgtcgtc cggcgtggga ggttcggacc cgtcgaagag gcagaccacg aggtgggcct
     1981 cctcgatggc cgtcatggtc tgacgcagca cgccctcgga gaggtcgata tcgcccctcg
     2041 tgtcgaaacc gcccgtgtca acgaggtcca cgtcgagccc tgccgccagg gtccacctcc
     2101 cgaagatgcg gtccctcgta atgccgcgct tcgggccgac gatggatttc cactcgccta
     2161 tgagcgcgtt gaagagcgtg gacttg
//
LOCUS       DAOSCR010000483         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_33036_length_2180_cov_2.987294, whole genome shotgun sequence.
ACCESSION   DAOSCR010000483 DAOSCR010000000
VERSION     DAOSCR010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33036_length_2180_cov_2.987294"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..734)
                     /locus_tag="PLT69_10635"
     CDS             complement(54..734)
                     /locus_tag="PLT69_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ14939.1"
                     /translation="MPRAQRTPEQIESVKKKILETAVELMNRVGYRDFSMRGLARELK
                     VSPPTLYTYFHDKDELYLCILIEGFKELYGLVEAECSRGGDPFGRLRGIARAYVDFGL
                     HRAHFYNLMFTWHVPKYNDYLGTPVEEVARLELETALGVARLVTGVIRECAGEGYTIT
                     EQDARFLLVCVWSTLHGFIAGINNTLLTYMHEDPLSLVDDLVDHVHERFVQDIRARMT
                     PRDRAAAR"
     gene            complement(1141..1416)
                     /locus_tag="PLT69_10640"
     CDS             complement(1141..1416)
                     /locus_tag="PLT69_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ14940.1"
                     /translation="MTKTGLVEVVAGKLKISKSAAQAAVDAMTDAISASLKKGEKVTL
                     VGFGTFDVVKRAARQGMNPRTREKIKIKACKAPRFKAGKEFKALVNK"
     gene            1644..>2180
                     /locus_tag="PLT69_10645"
     CDS             1644..>2180
                     /locus_tag="PLT69_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006706802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOJ14941.1"
                     /translation="MKRPGRTITLSKGGRAFVPDPLPPKLEWSHGLLRSLSEADRLLG
                     WLAGEGRRMKNPTLLMRPFLTREAVLSSRIEGTQATLGEILAADAGAGGGLGRGDLRE
                     VANYVACLNHGIARLGQTPLSTPLVLELHERLMDGVRGEDATPGEFRKVQNWIGPPGC
                     RIEEASYVPPPPELVPELM"
BASE COUNT          381 a          729 c          646 g          424 t
ORIGIN      
        1 tcatcgctac ctcctgtcat cctgcggcga gacagccgca cctcatcatc cgttcagcgg
       61 gccgccgccc tgtctctggg tgtcatcctg gcccttatgt cctgcacgaa gcgttcgtga
      121 acatgatcga cgagatcgtc cacgagcgac aagggatctt cgtgcatgta ggtgagcagg
      181 gtgttgttga tgcctgcgat gaagccgtgc agggtgctcc acacgcacac gagcaggaac
      241 ctcgcgtctt gttccgtgat cgtgtaaccc tcaccggcgc actccctgat gacccccgtg
      301 accagccggg ccacgccgag agcggtctcg agctcgagcc gggccacctc ttccacgggc
      361 gtacccaggt agtcgttgta cttgggcacg tgccacgtga acatgaggtt gtagaaatgc
      421 gcacggtgca gaccgaagtc cacatacgcc ctggcgatcc ccctgagccg cccgaagggg
      481 tcgccgcctc gcgagcactc tgcctcgacg agcccgtaca gctccttgaa cccctctatg
      541 aggatacaga ggtagagctc gtctttgtcg tggaaatagg tgtagagcgt gggcggggac
      601 accttcagtt cccttgccag ccccctcatg ctgaaatccc ggtagcccac ccggttcatg
      661 agctcgaccg ctgtctcgag gattttcttc ttgacgctct cgatctgctc gggtgttcgc
      721 tgtgcccggg gcatgcacgc tctccttgag ggccttcccg cctcgaggcc ggccgaccga
      781 accgccgact cccgcgaggg ccttccggat gtctttacat gtgttatatt aacacatgtc
      841 acctctacgg ccaaggggat cctcggtcac tccactgggt gctgtacctg tactgacata
      901 cggcggagcc cctcggtcgg aggggactcg gaacggggcg caggtgatca cgcggtcttc
      961 gactgcggcg cacctatcca cacgaggact tcaagggcca tccgacagcc cttcaacgat
     1021 tgggacaggc accgcccccg cggcccggag gcaccacgcc gcaggcaggg acggctggca
     1081 cggccgcctc ccgcccgcgc ccatccaggg cagccctgcc gagaggggga aggttccccc
     1141 ctacttgttc accagggcct tgaactcctt gcccgccttg aagcgcggcg ccttgcacgc
     1201 cttgatcttg atcttctcgc gggtcctcgg gttcataccc tgcctggccg ccctcttcac
     1261 cacatcgaac gtcccgaacc cgacgagggt gaccttctcg cccttcttga gcgaagccga
     1321 tatggcgtcg gtcatcgcgt ctactgctgc ctgagcggca gacttggaga ttttcaactt
     1381 gccggccacg acctcaacaa gacccgtctt cgtcatactt ccctcctctt atctgttggg
     1441 aatgtactga acgccgcagt tcaagatcgt gcaaatagta taatcgaccg cattcctgtt
     1501 tcaagccttt tttgatgccc gccttccgtt tccacccgtg acgacgagct tcttgataac
     1561 ttattatcgc tggtgttatg atctgaaaat aatcagcgcc gctgttttcg ccggcgaaaa
     1621 tggagaagac ctgggcggag gcgatgaaga ggccgggcag gacgataacg ctctcgaaag
     1681 gaggccgcgc cttcgtgccc gatccgctcc cacccaagct ggaatggtct catggcctgc
     1741 tccgctcgct ctcggaggcc gacaggctcc tggggtggct cgccggggag ggcaggcgca
     1801 tgaagaaccc caccctcctc atgcggccct tcctcacccg cgaggcagtc ctctcgagcc
     1861 gcatcgaggg cacgcaggcc accctggggg agatactggc tgcagacgcc ggcgcagggg
     1921 gaggcctggg ccgtggcgac ttgagggagg tggcgaacta cgtggcctgc ctgaaccacg
     1981 ggatcgcccg cctcgggcag acacccctgt cgaccccgct cgtcctggag ctccacgagc
     2041 gcctcatgga cggggtgagg ggggaagacg ccacgccggg tgagttcagg aaggtccaga
     2101 actggatagg cccgccgggc tgccgcatcg aggaggcgtc atacgtgccc ccgccccctg
     2161 aactcgtgcc cgaactcatg
//
LOCUS       DAOSCR010000484         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_33076_length_2178_cov_4.074894, whole genome shotgun sequence.
ACCESSION   DAOSCR010000484 DAOSCR010000000
VERSION     DAOSCR010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33076_length_2178_cov_4.074894"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(493..1455)
                     /locus_tag="PLT69_10650"
     CDS             complement(493..1455)
                     /locus_tag="PLT69_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ14942.1"
                     /translation="MNPLLQVRGITKTFELRKGIFGGPRGVVHAVDGVDLDLFPNETL
                     GLVGESGCGKSTLARLVLRLMEPDGGSVVFDGRDVLRARGRDLMECRRGMQVVFQDPF
                     GSLNPRMKVRDIIEEPMIVHRTGPRAWIGRRAEELASMVGLDGELLRRYPHEFSGGQR
                     QRICIARALAIEPRVVILDEPVSSLDVSIQAQVINLLTDLQERLRLSYLFISHDLSVI
                     GYLSHRVAVMEKGRIVELARCEDIFDDPLHPYTKALLEAVPDIEGPRFSPAVTSRPDP
                     DQEGHDLSGCVHAAVCPGARPRCSRERPVLVEVKPGHFAACHLL"
     gene            complement(1541..>2178)
                     /locus_tag="PLT69_10655"
     CDS             complement(1541..>2178)
                     /locus_tag="PLT69_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ14943.1"
                     /translation="EVFLLHRRTMARSYREALPLVIDMLATVGIKDPARRVHDYPHQL
                     SGGMRQRVMIAMAMACRPAVLIADEPTTALDVTTQRQILGLMDELRASSGTSIVFVTH
                     DLGIVAGFCDDVAVMYAGRVVESAPAGRLFEEPLHPYTKGLLKSVPRIGRSRRGKRLP
                     AIEGSVPTPSDMPGGCTFHPRCPEVMGVCRQEPPPAYEVGAGCVVRCWRYA"
BASE COUNT          338 a          756 c          725 g          359 t
ORIGIN      
        1 cacgtcggtc tggcgcatgg cggtgggtca cacgcggctt cgggcaggcc gaggtgagcg
       61 gactcagggc cggcgctcac ccggagacct cggcgcgcgg cctcaggcat gtggcgttcc
      121 ggatctccag gcctgtgcgc tcgggcccgg ggatcaacgc cgtgaggcag actctctctg
      181 cacgtggatg acgatatccc ccgcaccttg gcgggcccga ggcccgcacg acatccggat
      241 cccggtgcgg ggtgcatcgc acgacatcgg caccatgcgc cctcggcggc tcgaaggtct
      301 ccccgcatca tcacccctcc cagcgatctc ggggcttacg ggggctgctc ggacgccatg
      361 ggcgggcgcg tcccgacgtt gtggatcgtg ccccaggggc cgaaaggtcg actccttccc
      421 tcgttctccg tgatgttctc caattccgtc tggccgagcg ggaccgctcg ggctcaagag
      481 gcgtcctttc ggtcacagga ggtgacaggc ggcgaagtgc ccgggcttga cctcgaccag
      541 aaccggccgc tccctggaac acctcgggcg cgccccaggg cagacggcgg cgtggacgca
      601 cccggagagg tcatgccctt cctggtcagg gtcgggccgc gacgtgacgg ccggggagaa
      661 cctgggcccc tcgatgtcgg ggaccgcctc caggagcgcc ttggtgtacg ggtgcagggg
      721 gtcgtcgaag atgtcctcgc acctcgcgag ctccacgatg cgtccctttt ccatgaccgc
      781 cacccggtgc gagaggtacc cgatgaccga gaggtcatgg gagatgaaga ggtacgacag
      841 cctgagacgt tcctgcaggt cggtcaggag gttgatgacc tgggcctgga tggacacgtc
      901 gagagacgag acaggctcgt ccaggatcac gaccctgggc tcgatggcga gggccctcgc
      961 gatgcagatc ctctggcgct ggccgcccga gaactcgtgc gggtatctcc gcaagagctc
     1021 gccgtccagg cccaccatgg aggcgagctc ctccgccctg cgccctatcc aggccctcgg
     1081 gcccgtacgg tgcacgatca tgggttcttc gatgatatcg cggaccttca tcctggggtt
     1141 gagggaaccg aaggggtcct ggaagaccac ctgcatcccc ctcctgcact ccatgaggtc
     1201 ccggcctcga gcgcgcaaca cgtcgcgccc gtcgaacacc acgctccccc catctggctc
     1261 catcagcctc agcacgagcc tggcgagcgt cgatttcccg caaccggact cacccacgag
     1321 gccgagggtc tcgttcggga acaggtcgag atcgaccccg tcgacggcgt ggaccacacc
     1381 cctggggccg ccgaagatgc ctttcctcag ctcgaaggtc tttgtgatgc cccttacctg
     1441 caggagggga ttcatacgcc ctcgcacctc ccgtcgcttt cgggcttcct tgcgcctgcc
     1501 gtgcgcagcg cgtcactgga ggcacccgcg gtgttcacgg tcatgcatac ctccaacacc
     1561 tgaccacaca cccggcaccg acctcgtatg caggcggggg ctcctgcctg cagacgccca
     1621 tcacctccgg acacctcggg tggaaggtac agccgccggg catgtcgctc ggggtgggca
     1681 cgctgccctc gatggccggg aggcgttttc ccctgcgaga gcggccgatc ctgggaacgg
     1741 acttcaggag ccccttcgtg taggggtgga gcggctcctc gaagagcctc ccggcaggag
     1801 cgctctcgac gacgcggcct gcgtacatga ccgccacgtc atcgcagaac ccggcgacga
     1861 tcccgaggtc gtgggtcacg aagacgatgg acgtccccga ggacgcccgg agctcgtcca
     1921 tgaggccgag gatctgtctc tgcgtggtca cgtcgagggc cgtggtgggc tcgtccgcga
     1981 tcaacaccgc cggcctgcag gccatggcca tggcgatcat gaccctctgc ctcatcccgc
     2041 cgctcagctg gtgcgggtag tcgtgcaccc tcctggcggg gtccttgatg ccgaccgtgg
     2101 ccagcatgtc gatcaccagg gggagcgcct cgcggtagct cctcgccatg gtgcgccggt
     2161 ggaggaggaa gacctccg
//
LOCUS       DAOSCR010000485         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_33129_length_2176_cov_3.702499, whole genome shotgun sequence.
ACCESSION   DAOSCR010000485 DAOSCR010000000
VERSION     DAOSCR010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33129_length_2176_cov_3.702499"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLT69_10660"
     CDS             <1..627
                     /locus_tag="PLT69_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOJ14944.1"
                     /translation="SVRHITIKGAFHVKSDDALGLMDIGEGDNILTWNMHEARRMLEA
                     HPWIEKVRISRSFLPASLEVEITERRPEATLILEDKPYLVTGDGVPFLASPEVFYGLV
                     IHADGFTKDEMGSGLSDAIKHALAWARLAGSRGLDVLDVAIEPGFLMNMRLKNSLVFT
                     VFGAPNPVTLDRALKVIKAIKPVEGTIMDLRCQDKIVLRTRGAHGGEG"
     gene            611..1837
                     /gene="ftsA"
                     /locus_tag="PLT69_10665"
     CDS             611..1837
                     /gene="ftsA"
                     /locus_tag="PLT69_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOJ14945.1"
                     /translation="MAARDNIIVGLDIGTTKICCLITEVNETGELEVIGFGVSESKGL
                     RRGLVVNMEQTVKAIKSCVEDAERMAGITVDSVYTGIAGGHIQGISTGAVIAIKGDEI
                     TEADQRRVVDQAKEFAKPQGVEIIHVLQQEFIVDDVGGIVNPVGMAGSRLEAKVHIVT
                     GSISAVSNITKCAQKAGLGLKDIVLQQIASAAATLTPDETKLGVILMDIGGGTTDIAV
                     FNDGSIRHTSVLALGGDNITNDIAVGLRCPIAEAERIKIRYGHAISSKVSSEEQIEVP
                     SIGGDTVNMVPRRILAEIIESRVEELIKLAYNEITNTGYSDMLSAGLVLTGGTSMLEG
                     VAELAESVMHMPVRVGMPRGVKGLVELVNNPAYATAVGLCVYEASKPAHSLKPRGAGI
                     FERILERMMSWFRESF"
     gene            1854..>2176
                     /locus_tag="PLT69_10670"
     CDS             1854..>2176
                     /locus_tag="PLT69_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ14946.1"
                     /translation="MFELEKIDSKGAQLIKVVGVGGCGCNAVNNMINAKIKGVEFIAC
                     NTDIQSLRSSLSTSRIQLGVKLTKGLGAGGDPEVGKNAAIENEKDIRAAIENADMIFI
                     AAGMGG"
BASE COUNT          470 a          608 c          752 g          346 t
ORIGIN      
        1 tcggtgaggc acataacgat caagggcgca ttccacgtga agtccgacga tgcgctcggg
       61 ctcatggaca tcggggaggg agacaacatc ctcacctgga acatgcacga ggcgaggagg
      121 atgctggagg ctcacccctg gatcgagaag gtcaggatct ccaggtcctt cctccccgca
      181 tccctggagg tggagattac cgagcgcagg cccgaggcca cgctcatcct cgaggacaag
      241 ccgtatctcg tcaccggtga cggggtgcct ttcctcgctt cgccggaggt cttctacggc
      301 ctcgtgatcc acgccgacgg gttcacgaaa gacgagatgg gaagcgggct ttcggatgcc
      361 ataaagcatg cgctcgcgtg ggcgaggctc gccgggtcga gggggctcga cgtgctcgac
      421 gtcgccatag aacccgggtt cctcatgaac atgaggctga agaactcgct cgtgttcacg
      481 gtgttcggcg cgccgaaccc cgtcacactg gaccgggccc tgaaggtgat caaggcgatc
      541 aaacccgtgg aggggactat catggacttg agatgccaag acaagatcgt gctgcggaca
      601 aggggtgctc atggcggcga gggataacat cattgtgggt ctcgacatcg ggacgacgaa
      661 gatctgctgc ctcatcaccg aggtcaacga gaccggcgag ctggaggtga tcggtttcgg
      721 ggtgtccgag tccaagggcc tcaggcgggg gctcgtggtc aacatggaac agaccgtgaa
      781 ggccatcaag agctgcgtgg aggatgcgga gcgcatggcc gggatcaccg tggacagcgt
      841 gtacacgggc atagccggcg gtcatatcca ggggatcagc acgggcgccg tcatcgccat
      901 caagggcgac gagatcacgg aggcagacca gcgcagggtc gtggaccagg cgaaggagtt
      961 cgccaagccg cagggggtgg agatcatcca cgtcctccag caggaattca tcgtggacga
     1021 cgtgggaggg atcgtgaatc ccgtgggcat ggccgggtcc cggctcgagg ccaaggtgca
     1081 catcgtcacg gggtcgatct cggcggtgag caacatcacc aagtgcgcgc agaaggccgg
     1141 cctgggcctg aaggacatcg tgctccagca gatcgcctcc gccgcggcga ccctcacccc
     1201 cgacgagacg aagctcggcg tcatactcat ggacatcggc ggggggacga cggacatagc
     1261 ggtcttcaac gacggctcca tacgtcacac gtccgtgttg gccttgggcg gcgacaacat
     1321 aacgaacgac atcgcggtgg ggctgcgctg ccccatcgcc gaggcggaac ggatcaagat
     1381 caggtacggc cacgccatct cctccaaggt gtcgtcagag gaacagatcg aggtcccctc
     1441 cataggcggg gacacggtga acatggtgcc gaggcggatc ctggccgaga tcatcgaatc
     1501 gagagtggaa gaactcatca aactcgcata taatgagatc acgaacacgg ggtactcgga
     1561 tatgctgtcc gcagggctcg ttctcacggg agggacgagc atgctggagg gggttgccga
     1621 actcgccgag agcgtcatgc acatgccggt acgcgtcggt atgccgcggg gcgtgaaggg
     1681 gctggtggaa ctcgtcaaca acccggcgta cgccacggcc gtcggcctgt gcgtgtacga
     1741 ggcgtcgaaa cctgcgcaca gtctcaaacc caggggtgcg ggaatcttcg agaggatcct
     1801 cgagaggatg atgagctggt tcagggaatc cttttaacgt cagggggggg gcaatgttcg
     1861 aactcgagaa gatagacagc aagggcgctc agctcataaa ggtcgtcggg gtcggcgggt
     1921 gcggctgcaa cgccgtgaac aacatgatca acgcgaagat caagggtgtg gagttcatcg
     1981 cgtgcaacac cgacatacag tccctgagat cgtcgctgtc cacgtccagg atccagctcg
     2041 gggtgaagct caccaagggc ctcggcgccg ggggggaccc ggaggtcggc aagaacgcgg
     2101 cgatcgagaa cgagaaggac atcagggcgg cgatcgagaa cgccgacatg atcttcatag
     2161 cggccggcat gggcgg
//
LOCUS       DAOSCR010000486         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_33301_length_2167_cov_2.997159, whole genome shotgun sequence.
ACCESSION   DAOSCR010000486 DAOSCR010000000
VERSION     DAOSCR010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33301_length_2167_cov_2.997159"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /gene="glgP"
                     /locus_tag="PLT69_10675"
     CDS             complement(<1..1685)
                     /gene="glgP"
                     /locus_tag="PLT69_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ14947.1"
                     /translation="MADMENHLHDRLKALAQNLWWTWHPEVISLFTDIDPHLWRVVEH
                     NPIAFLNRITPEQVEERASELVLHSRINHAFRRLNEYMEQERTWGSINCGNVNYRPVV
                     YFSAEFGLHESLPIYSGGLGILAGDHLKSASDLGIPLIGIGLLYQQGYFNQRLNQNDL
                     QEEDYVDLDLENLPLHPVLNEHGTQLTVRVDTRTTPIFARVWRLQVGRVRLLLLDTNV
                     EANTLEDRSLTNRLYGGDTRVRIRQELLLGIGGARVLQALHIHPGVLHLNEGHSAFAI
                     LEEIRRTMEYDMIPFNRAMRRVSLYTVFTTHTPVAAGHDRFSPDLVEEHLGVFRERLG
                     LPHDEFMALGRVNPNDAGEPFCMTVLALKGSRRANAVSAIHGTVSRLMWRGLWPNRPE
                     SEIPIGHITNGVHVLSWLAPQMYQLFETHLGSDWATRMVHPDVWEGVEKIHDGELWET
                     HQFLKMRLIRFVRRRLRIQAERLGLKHILKDIDHVLDPDVLTIGCARRFAAYKRGDLV
                     VSDLERLTKLIKDTKRPIQIIFAGKAHPNDDEGKRLLQKIANLSHDKKYTGSIV"
     gene            complement(1881..>2167)
                     /locus_tag="PLT69_10680"
     CDS             complement(1881..>2167)
                     /locus_tag="PLT69_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14948.1"
                     /translation="LMEKSHVACPVCSGNGLVRSVESSAMAALRQIQSSLSRSRAPRV
                     DVTLSPEVAMHLVNECRRHIDALESEFKTRIHLKVDEGVGRPFCAVTHAE"
BASE COUNT          354 a          657 c          705 g          451 t
ORIGIN      
        1 acgatggagc ccgtgtactt cttgtcgtga gagaggttcg ctatcttctg gagcaaccgc
       61 ttcccttcgt cgtcattggg atgcgccttt ccggcgaaga tgatctggat ggggcgtttc
      121 gtgtccttga tgagcttggt gaggcgctcc aggtccgaga cgacgaggtc gccccgcttg
      181 tacgcggcga atctcctggc gcagcctatg gtgaggacgt ccgggtccag cacgtggtcg
      241 atgtccttga ggatgtgttt caagccgagg cgttccgctt ggatgcgaag cctcctcctc
      301 acgaaccgga tgaggcgcat cttgaggaac tggtgggtct cccagagctc cccgtcgtgg
      361 atcttctcca cgccctccca gacgtccggg tggaccatgc gcgtcgccca gtcggaaccg
      421 aggtgggtct cgaagagttg gtacatctgg ggggcgagcc acgacagcac gtgtacgccg
      481 ttggtgatgt ggcctatggg gatctccgac tccggcctgt tcggccagag ccctctccac
      541 atgagcctgc tcaccgtgcc gtggatggcc gagacggcgt tcgccctcct cgaacccttg
      601 agggcgagga ctgtcatgca gaagggttcc cctgcatcgt tcgggttcac gcggcccagg
      661 gccatgaact cgtcatgggg aagaccgagc ctctctcgga acacgccgag gtgttcctcg
      721 acgaggtcgg gcgagaagcg gtcgtggccg gcagccacag gggtgtgcgt cgtgaacacg
      781 gtgtagaggc tcacccttcg catggcccgg ttgaagggga tcatgtcgta ttccatggtc
      841 ctgcgtatct cctcgaggat ggcgaacgcg ctgtgacctt cgttcaggtg cagcacgccg
      901 ggatggatgt gcagggcctg gagcacgcgg gcgcccccga taccgaggag aagctcctgc
      961 ctgatgcgga ccctcgtgtc cccaccgtag agcctgttgg tgagcgagcg gtcctccagg
     1021 gtgttggcct ccacgttcgt gtcgaggagc aacagcctga ctcgtcccac ctggagcctc
     1081 cacacgcggg cgaagatcgg ggtggtcctg gtgtcgaccc gcacggtgag ctgggtcccg
     1141 tgttcgttca agaccgggtg gagggggagg ttctcgaggt cgagatcgac atagtcctct
     1201 tcctgcaagt cgttctggtt gagcctctgg ttgaagtatc cctgctggta gagcagcccg
     1261 atgccgatca aggggatgcc gaggtcggat gcgctcttga ggtgatcccc cgccaggatg
     1321 cccaggccgc cggaatagat ggggagcgac tcgtggagac cgaactccgc cgagaaatag
     1381 accaccggcc ggtagttgac gttcccgcag ttgatggaac cccaggtgcg ctcctgttcc
     1441 atgtattcgt tcaaccgcct gaacgcgtgg tttatcctgc tgtgcaggac gagttcggat
     1501 gcgcgttcct ccacctgttc aggggtgatc ctgttgagga acgcgatggg gttgtgttcc
     1561 accacgcgcc aaaggtgggg gtcgatgtcg gtgaacaggg atatcacctc cgggtgccac
     1621 gtccaccaga ggttctgtgc cagggccttg agcctgtcgt gcaggtggtt ctccatatcg
     1681 gccatgcgac ctcctccccg tgtaggtctt tcgtattgca aaggggtatg gcatcgtgcc
     1741 ccaccggtcc tcctcgcagg accacaggtg aaaaggcatc gccacgcccc ggtacgcctt
     1801 ggcaaaggcc ttcttccagc agctgtcgcc ccatgcccgt gtggttcgtc cccccttgga
     1861 gctcacctgg gagggccgac tcattccgca tgcgtcaccg cgcagaatgg tcttcccaca
     1921 ccctcgtcca ccttcaggtg gattctcgtc ttgaactcgc tttccagggc atcgatgtgc
     1981 cgcctgcact cgttcacgag gtgcatcgcc acctccgggc tcagggtgac atcaacccgg
     2041 ggcgcccggg atctggacag cgaggactgg atctgcctca aggcggccat ggcggaggat
     2101 tccacggaac ggacgagccc gtttcccgag cacacggggc aggccacgtg gctcttttcc
     2161 atcaggg
//
LOCUS       DAOSCR010000487         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_33893_length_2143_cov_3.667625, whole genome shotgun sequence.
ACCESSION   DAOSCR010000487 DAOSCR010000000
VERSION     DAOSCR010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33893_length_2143_cov_3.667625"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1059)
                     /locus_tag="PLT69_10685"
     CDS             complement(139..1059)
                     /locus_tag="PLT69_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ14949.1"
                     /translation="MDTGPDTRIRCVMERMHEVYLGRDEVISCILAALLAGGHVLLEG
                     VPGVGKTLIAITLSRLFDLSFKRIQFTNDMLPSDVTGFHTLRGETGAWEMVKGPIFAS
                     IVLADEINRTSPKTQSALLEAMEEAQVTIDGVTYELPRPFMVIATQNPVEIAGTFPLP
                     ESQLDRFLMKLSVEYPPLKEEREILMKDIDHADALKLRPVMTKDELVDLADEASTVSV
                     HPDVISYIVNIVRATRVHPRVELGVSPRGGLALKRAAQAWAYISGRNFVTPGDVATLA
                     PFVLSHRIISKDGDPGGLVEEVLREVEVPL"
     gene            complement(1177..1371)
                     /locus_tag="PLT69_10690"
     CDS             complement(1177..1371)
                     /locus_tag="PLT69_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14950.1"
                     /translation="MINLEVTKDELELLKMLLEREHVNTRIEIHHARMSFEYRDFLKK
                     RVKEIEGLLDKICTILPEAA"
     gene            1533..>2143
                     /locus_tag="PLT69_10695"
     CDS             1533..>2143
                     /locus_tag="PLT69_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ14951.1"
                     /translation="MADERYRKILDDMQEGYYEIDLNGTFTFVNDVVASIAEIPKEEL
                     IGLNYIEYTSPATAKRVFKTFNKVFQTGIPQRIEYEIILRDNRKKIIENSVSLLKDSE
                     NNVIGFCGLVTDITKRKRLEEQIKESRQRFEALFENANELIITTDAQGYIKRLNKKVE
                     EISGYRREELIGKSILVLAHPDDRLLYIDFWKQLLDGKTPRFEL"
BASE COUNT          435 a          677 c          628 g          403 t
ORIGIN      
        1 cctccgaggg aacgacggtg cccgtcgttc acctccatcg aacgctcgaa cgcgtctcgg
       61 tgcgctcgag gacgggagcc gcacccgcgg cgcatcacct cgactcccac cctccggcca
      121 ggccgggtga acccgacttc acaggggaac ctcgacctcg cggagtacct cctccacgag
      181 ccctcccggg tcaccgtctt tggagatgat acggtgggag agcacgaaag gcgcgagtgt
      241 cgccacgtcc cccggcgtca cgaaattcct tccgctgatg taggcccatg cctgcgcggc
      301 cctcttgagg gcgagaccgc ccctggggga cacgccgagc tccacgcggg gatggacccg
      361 cgtcgccctg acgatgttca cgatgtacga gatcacgtcg gggtgcaccg acaccgtgga
      421 ggcctcatcg gcgaggtcga cgagctcgtc tttcgtcatg accggcctca gcttcaaggc
      481 gtcggcgtga tcgatgtcct tcatcaggat ctccctctcc tccttcagag gcgggtattc
      541 cacggagagc ttcatgagga acctgtccag ctggctctcg ggcaggggga acgtaccggc
      601 tatctcgaca gggttctgcg tcgcgatgac catgaagggt cggggcagct cgtacgtaac
      661 cccgtcgatg gtcacctgcg cctcctccat ggcctccagc agggcggact gggtcttggg
      721 cgaggtcctg ttgatctcgt ccgcgagcac gatgctcgcg aagatcggcc ccttgaccat
      781 ctcccaggcc cccgtctcgc ccctcaacgt gtggaaaccg gtgacatccg aaggcaacat
      841 gtcgttggtg aactgtatgc gcttgaagct caggtcgaac aggcgggaaa gggtgatggc
      901 gatgagcgtc ttgcccacac cgggcacacc ctccagcagg acgtgtccgc cggcgagcag
      961 ggcggccagg atacaggaga tgacctcgtc gcggccgagg tacacctcgt gcatcctctc
     1021 catcacgcac ctgatcctcg tatcgggacc cgtgtccatg ccctgctcct tagtttcccc
     1081 gcgtcagact accacagggg caggtcggtg aacacggcca ttgacgtgtc tcgccttgag
     1141 ggggcggcat gcaggcctac acggccccca tacgcctcag gccgcttccg gcaggatcgt
     1201 gcagatcttg tcgaggagcc cctcgatctc cttgaccctc ttcttcagga aatcgcggta
     1261 ttcgaacgac atccgcgcat ggtggatctc gatcctcgtg ttgacgtgct cccgttcgag
     1321 gagcatcttg aggagctcca gctcgtcctt cgtcacctcg aggtttatca tggcgacctc
     1381 ctttcggctc ggggccttca cccatccagg gacaccgcac ccctcacgtt tcattcatct
     1441 ttatcgttca taataacgaa ctgttgccct gagggaagat ctggtattaa gtattctctc
     1501 gcgttttccg atttccaaca ggaagggcgg gtatggctga cgaacggtac cgcaagatcc
     1561 tcgatgacat gcaggaaggc tactacgaga tcgatctcaa cggtaccttc accttcgtca
     1621 acgacgtcgt ggcttcgatc gccgagatcc ccaaggaaga actcataggc ctcaactaca
     1681 tcgagtacac gagcccggcc actgcgaaac gcgtgttcaa gaccttcaac aaggtattcc
     1741 agacagggat cccccagcgg atcgagtacg agatcatcct gagggacaac cggaagaaga
     1801 tcatcgaaaa ctcggtgagc ctgctcaagg acagcgagaa caacgtcata gggttctgcg
     1861 ggctggtgac ggacatcacg aagaggaagc gcctcgagga gcagataaag gagagcaggc
     1921 agcggttcga ggccctcttc gagaacgcca acgaactcat catcaccacg gacgcccagg
     1981 ggtacataaa gcggctgaac aagaaggtgg aggagatatc ggggtaccgg agggaggagc
     2041 tcatagggaa gagcatcctc gtgctcgccc acccggatga ccgtctcctc tacatcgact
     2101 tctggaagca gctcctggac ggcaagacac cgcggttcga gct
//
LOCUS       DAOSCR010000488         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_33951_length_2141_cov_3.332694, whole genome shotgun sequence.
ACCESSION   DAOSCR010000488 DAOSCR010000000
VERSION     DAOSCR010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33951_length_2141_cov_3.332694"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLT69_10700"
     CDS             <1..998
                     /locus_tag="PLT69_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOJ14952.1"
                     /translation="PMDGKGPIPAQGQYPVNGDPPPALSRRKIQQILPLGVRVLDGLI
                     TCGVGQRLGIFAGPGVGKSSLLGMIARHTKADVNVIALIGERGREVREFLERDLGEDG
                     LRRSVVVAAVSDQPSLVRIRGAYLATTIAEFFRDQGQDVLLMMDSVTRFAMSMREIGL
                     AVGEPPTTKGYTPSCFARLPKLMERVGNTSSRGSITGLYTVLVEGDDFNEPVADAVRS
                     IVDGHIVLSRDLASRAHYPAVDILRSVSRVMNDIASETHKKAARRILALTAAYEEARD
                     HLNIGAYVPGSNKEIDDAVKHIDAIKGFLRQDINEKSPLEDTVNRMLALAGDAGR"
     gene            1012..1452
                     /gene="fliJ"
                     /locus_tag="PLT69_10705"
     CDS             1012..1452
                     /gene="fliJ"
                     /locus_tag="PLT69_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOJ14953.1"
                     /translation="MAFTFKFSTLLKVRKIQEDLARQQLAEAARQLTSILALRSQVEA
                     RKALLSAELLAAMSDGMKAHEVKAVSDYLYHLEERLVRLQDAVEKATMRVEEEREKVV
                     KARKAHKAIERLREIHLERYKAEQDRLEMNFIDEIAVMGAGGRV"
     gene            1449..2015
                     /locus_tag="PLT69_10710"
     CDS             1449..2015
                     /locus_tag="PLT69_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14954.1"
                     /translation="MKTKIVLTLAVINGVLIALFFFMKPARLDAQNEEAQVKAAQKPA
                     PAIAVQDAMGFSVDDRIEVLRQKEQRLSEREAQLKELERQIQERIKRLEELQSAIRND
                     LASYRVVSQERVKHLVKIYSSMKPTAAASLMNNLETDVAVEVFLGMKGDVAGGILSYM
                     DPSKAALITQRLVTYRGSTKASAETEAR"
BASE COUNT          448 a          677 c          714 g          302 t
ORIGIN      
        1 aacccatgga cggaaagggt cccatcccag cgcaaggcca gtacccagtg aacggagacc
       61 caccgcccgc tctctctcgg cgcaagatcc agcagatcct cccgctgggc gtacgggtcc
      121 tcgacggcct catcacgtgc ggagtgggcc aacgcctggg catcttcgcc ggaccgggcg
      181 tgggcaagag ctccctcctg ggcatgatcg cccgccacac gaaagcggat gtgaacgtga
      241 tcgcgctgat cggggagcgg ggacgggagg tcagggagtt cctcgagagg gacctgggcg
      301 aggatgggct caggcgctcg gtggtcgtcg ccgcggtctc cgaccagccg tcgctcgtgc
      361 gcatccgggg ggcctacctc gcgaccacca tcgccgagtt cttccgggac caggggcagg
      421 acgtcctgct catgatggac tcggtgacca ggttcgccat gtccatgagg gagatcggcc
      481 tcgccgtggg agaaccgccc acgaccaagg ggtacacgcc ttcctgtttc gcgaggctcc
      541 ccaagctcat ggagcgcgtc gggaacacct cgtccagggg gagtatcacc gggctgtaca
      601 ccgtgctcgt ggaaggcgac gatttcaacg agcccgtggc ggacgcggtc agatccatcg
      661 tggacggtca catcgtcctc tcccgcgatc tcgcctcgcg cgcgcactac cccgcagtgg
      721 acatcctgag gagcgtgagc agggtcatga acgacatcgc gagcgagacc cacaagaagg
      781 ccgcccgccg gatcctggcg ctgaccgccg cctacgagga ggcgagggat cacctcaaca
      841 taggggcgta cgtccccggt tccaacaagg agatagacga cgccgtcaag cacatagacg
      901 cgatcaaggg gttcctcagg caggacatca acgagaaatc ccccctcgaa gacaccgtga
      961 acaggatgct ggccctcgca ggggatgccg gccgttgagc gggggcgagc catggcgttc
     1021 accttcaagt tctccaccct cctgaaggtt cgcaagatcc aggaagacct ggcaaggcag
     1081 cagctggccg aggccgccag gcagctcacc tcgatcctcg ccctgaggtc ccaggtagag
     1141 gcgagaaagg ccctcctctc ggccgagctc ctggccgcga tgtcagacgg gatgaaggcc
     1201 cacgaggtga aggccgtgtc cgactacctg taccacctcg aagagaggct cgtgcgcttg
     1261 caggacgccg tcgagaaggc caccatgcgc gtggaggaag agcgggagaa ggtggtgaag
     1321 gctcgcaagg cccataaggc catcgagagg ctcagggaga tccacctcga acggtacaag
     1381 gccgaacagg acaggctgga gatgaacttc atagacgaga tcgcggtcat gggagcagga
     1441 ggaagggtat gaagacaaag atagtcctca cgctggcggt gatcaacggg gtgctcatcg
     1501 ccctgttctt cttcatgaaa ccggcgaggc tcgacgccca gaacgaggag gcgcaggtca
     1561 aggccgcgca gaaacccgca ccggcgatcg cggtgcagga cgccatgggg ttcagcgtcg
     1621 acgaccgcat cgaggtgctc aggcagaaag agcaacgctt gagcgagcgg gaggcgcagc
     1681 tcaaggagct cgaacggcag atccaggagc ggatcaagcg gctcgaggag ctccagtcgg
     1741 ccatccggaa cgacctggcg agctaccggg tggtcagcca ggaaagggtc aagcacctgg
     1801 tgaaaatcta ctcgtccatg aagcccaccg ccgccgcgtc gctcatgaac aacctggaga
     1861 cggacgtcgc ggtggaggtc ttcctgggga tgaagggcga cgtcgcgggg ggcatcctct
     1921 cgtacatgga cccctcgaag gccgctctca tcacccagcg cctggtcacg taccggggga
     1981 gcacgaaggc ctcggccgag acggaggcca ggtagacccc tttccctgcg cgaagctcac
     2041 gtgcctcgat ccaagcacat ctgacgacgg cctaccaaaa cgggcgcggg gacgtcccgg
     2101 cgccccggac acgacgagac ccatcccctc cgggtatacg g
//
LOCUS       DAOSCR010000489         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34087_length_2136_cov_3.269101, whole genome shotgun sequence.
ACCESSION   DAOSCR010000489 DAOSCR010000000
VERSION     DAOSCR010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34087_length_2136_cov_3.269101"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLT69_10715"
     CDS             complement(<1..223)
                     /locus_tag="PLT69_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14955.1"
                     /translation="MFTEFFYLLREQGVPVTPTGFLKLHEALAKGCITSLEDFYAVTR
                     AVLVKSERLFDLYDRVFAHVFKGARLESQL"
     gene            complement(245..1081)
                     /locus_tag="PLT69_10720"
     CDS             complement(245..1081)
                     /locus_tag="PLT69_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ14956.1"
                     /translation="METREFTGGARYVLDAELSRIVNASIKLEMPLLVKGEPGTGKTL
                     LAHAIAENLSMPLIVLNVKSSMKAVDALYQYDTLTRLNDSRFGDSKRDVSNIEDYIKM
                     GKIGQAFVSDCKTVLLIDEIDKADTDFQDDLLDVLDQMSFDIIEIDKTVRAKHRPVVV
                     ITSNAKKDLSDPFLGRCIFHHIAFPEPEMMEKIVKAHFPDLDRRVSQACIETFYRLRN
                     LRGVEKKPATRELINWIRILLMDPSFDPKHLVRGEVPYLGVLFKKSEDFKIAQRLTGS
                     GS"
     gene            1234..2100
                     /locus_tag="PLT69_10725"
     CDS             1234..2100
                     /locus_tag="PLT69_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOJ14957.1"
                     /translation="MDRKEDAKPADTLMRRLWLYLTRTGEGERADDTRRQIEEILDEV
                     EEKGIIDEDQGDMIHNILFLRDTTASEIMVPKGDIVALSVDADLDDAARVFTEEGYSR
                     VPVYEGTMDNIVGILHAKDLLRYWKDRDDLPGIRSLLHEPFFVPEGKRLTDLIKEFRE
                     KRTKIAIVIDEYGGVDGMVTVADVMEEIIGDAIGEEGDDFEGYVEPLGEGLYAVDPKM
                     PVDEFAETFGISVPEGDYDTVAGFVTSSLQRIPRVGELFEHAGVVLEIAGADRRRISK
                     LVVRVPGKKDQA"
BASE COUNT          395 a          619 c          715 g          407 t
ORIGIN      
        1 ccagctggga ctcgagccgc gcccccttga agacgtgggc gaagaccctg tcatagaggt
       61 cgaagaggcg ctccgacttg acgagcaccg ccctcgtcac ggcgtagaag tcttccagcg
      121 aggtgatgca gcccttcgcg agggcctcgt ggagtttgag gaacccggtg ggggtcaccg
      181 ggacgccctg ttccctgagg aggtagaaga actccgtgaa cacggcccgt taccgcccgc
      241 ccccttatga tcccgagcct gtgagacgct gcgcgatctt gaagtcctcg ctcttcttga
      301 agaggacacc caggtacggg acctcgcccc gcacgaggtg tttcgggtcg aaggacgggt
      361 ccatgagcag gatcctgatc cagttgatga gctccctggt ggccggcttc ttctcgacgc
      421 ccctgaggtt cctcagccgg tagaaggtct cgatgcacgc ttgtgacacc ctgcggtcga
      481 ggtccgggaa gtgcgccttg acgatctttt ccatcatctc gggctcgggg aaggcgatgt
      541 ggtggaagat gcagcgtccg aggaacgggt cggagaggtc tttcttggcg ttgctcgtta
      601 tcaccacgac cggccggtgc ttggcgcgca cggtcttgtc gatctcgatg atgtcgaagg
      661 acatctggtc gagcacgtcg aggaggtcgt cctggaagtc ggtgtccgcc ttgtctatct
      721 cgtcgatgag caggacggtc ttgcagtcgg acacgaaggc ctggccgatc ttgcccatct
      781 tgatgtagtc ctcgatgttg ctcacgtcac gctttgagtc tccaaagcgg ctgtcgttga
      841 gcctcgtgag ggtgtcgtac tggtacaggg cgtcgacggc cttcatgctg gatttcacgt
      901 tgaggacgat gagcggcatc gacaggttct ccgcaatggc gtgcgccagc agggtcttgc
      961 ccgtcccagg ctcgcccttg acgagcagcg gcatctcgag cttgatgctg gcgttgacga
     1021 tccgggagag ttctgcatcc agtacgtatc tcgcgcctcc ggtaaactcg cgggtctcca
     1081 tggtaggttc tcctggtttc gtgggtgatc cctatcctta ccacacctgg ccctctcgtg
     1141 aaagaccgtc cgcgtcccgg gggtccattg actgggtccc ttgttttggt tacaagggga
     1201 attcgtagcc tagatgacgg gagcgaaggc cgtttggaca gaaaagagga tgcgaagcct
     1261 gccgacaccc tgatgaggcg cctctggctc tacctcacgc gcacgggcga gggggagcgg
     1321 gccgacgaca cccgcaggca gatcgaggag atcctcgacg aggtcgagga gaagggcatc
     1381 atcgacgagg accaggggga catgatccac aacatcctgt tcctcaggga caccactgcg
     1441 agcgagatca tggtccccaa gggcgatatc gtcgcgctga gcgtggacgc cgacctcgac
     1501 gacgccgcga gggtcttcac cgaggaagga tactccaggg ttcccgtcta cgagggcacg
     1561 atggacaaca tcgtggggat cctccacgcc aaggacctcc tcaggtactg gaaggacagg
     1621 gacgatctgc cgggcatccg gtccttgctc cacgagccct tcttcgtccc cgagggcaag
     1681 aggctcaccg acctcatcaa ggagttccga gagaagagga ccaagatcgc catcgtgatc
     1741 gacgagtacg gcggggtgga cggcatggtg accgtggccg acgtcatgga agagatcatc
     1801 ggcgacgcga taggcgagga gggggacgac ttcgaggggt acgtggagcc cctgggtgag
     1861 ggactctatg cggtggaccc caagatgccg gtcgacgagt tcgcggagac cttcggtatc
     1921 agcgtgccgg agggagacta cgacacggtg gcgggtttcg tcacgtccag cctccagcgg
     1981 atcccccggg tcggggagct cttcgagcac gccggcgtcg tgctggagat agcgggcgcc
     2041 gacaggagga ggataagcaa gctcgtcgtc agggtgcccg gcaagaagga ccaggcgtga
     2101 ggttccccgc cctccttgca tcgaccggtg cagacg
//
LOCUS       DAOSCR010000490         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34120_length_2134_cov_3.669553, whole genome shotgun sequence.
ACCESSION   DAOSCR010000490 DAOSCR010000000
VERSION     DAOSCR010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34120_length_2134_cov_3.669553"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="PLT69_10730"
     CDS             complement(<1..1739)
                     /locus_tag="PLT69_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOJ14958.1"
                     /translation="MAVRIGLASPEKILAWSYGEVKKPETINYRTFKPERDGLFCARI
                     FGPIKSYECICGKYKRMKHRGVVCEKCGVEVIDSRVRRERMGHIELATPVAHIWFLKS
                     LPSKIGALLDLSIKELERVLYFESYIVTDPKNTPLALGELLTEDKFRQAVDKYGIGSF
                     TAKMGAEAIDELIGSLDILELSSRLKEEIQKTKSEAKKKKLSRRLRMVNVFRGIEPAD
                     LMAFIEDRFHLEKDEQFRRALGELLTECRKVVKRWEEANGYQEKKAVIPKVNEVIERL
                     VESRKLSHDEIDYIKPWQDPSWMILKVIPVLPPDLRPLVPLDGGRFATSDLNDLYRRV
                     INRNNRLKRLIELNAPDIILRNEKRMLQEAVDVLFDNGRRGRVITGANRRPLKSLSDM
                     LKGKQGRFRQNLLGKRVDYSGRSVITAGPDLRLHQCGLPKKMAIELFKPFIYNKLEQK
                     SIVSTIKSAKRMVESETPEVWECLAEVVQEYPVILNRAPTLHRLGMQAFEPILIEGKA
                     IQLHPLVCPAFNADFDGDQMAVHVPLSIEAQVEARVLMMSTNNILSPAHGKPIIGPTQ
                     DIVLGMYYLTREKP"
     gene            complement(1803..>2134)
                     /locus_tag="PLT69_10735"
     CDS             complement(1803..>2134)
                     /locus_tag="PLT69_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14959.1"
                     /translation="LVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAIEAY
                     GAAYTLQEFLTVKSDDVAGRTRMYESIVKGQHELEPGIPESFNVLTRELKSLGLDISL
                     IEGQVQH"
BASE COUNT          329 a          664 c          684 g          457 t
ORIGIN      
        1 ggtttctcgc gggtgaggta gtacatgccg agcacgatgt cctgcgtcgg accgatgatg
       61 ggcttcccat gcgcgggcga caggatgttg ttggtggaca tcatgaggac gcgggcctcc
      121 acctgggcct cgatggacag ggggacatgc accgccatct ggtcgccgtc gaagtcggcg
      181 ttgaacgccg ggcacacgag ggggtgaagc tgtatggcct tgccctctat gaggatgggc
      241 tcgaaggcct gcatgccgag ccggtgcagg gtgggtgcgc ggttgaggat caccgggtat
      301 tcctggacga cctcggccag gcattcccag acctcgggcg tctcgctctc caccatccgc
      361 ttggcgctct tgatggtgct cacgatgctc ttctgctcga gcttgttgta gatgaagggc
      421 ttgaagagct cgatggccat cttcttgggc aggccacact ggtggagcct caggtcgggc
      481 ccggccgtga tgaccgagcg gcccgagtag tccacccgct tgcccaggag gttctggcgg
      541 aagcgcccct gcttgccctt gagcatgtcc gagaggctct tgagcggcct gcggttcgcg
      601 ccggtgatga cgcggccgcg cctgccgttg tcgaacagga cgtccaccgc ctcctgcagc
      661 atgcgtttct cgttgcgcag gatgatgtcg ggggcgttca gctcgatgag cctcttgagc
      721 ctgttgttgc ggttgatgac cctcctgtag agatcgttca ggtcggaggt cgcgaacctg
      781 ccgccgtcga gcggcaccag gggcctgagg tcgggcggca acaccgggat gaccttgagg
      841 atcatccagg aggggtcctg ccagggcttg atgtagtcga tctcgtcgtg ggacagcttg
      901 cgcgattcca cgaggcgctc gatcacctcg ttcaccttgg ggatgaccgc cttcttctcc
      961 tggtacccgt tcgcctcctc ccagcgcttg acgaccttgc ggcactccgt gagcaactcg
     1021 cccagggccc tcctgaactg ctcgtccttc tccaggtgga agcggtcctc gatgaaggcc
     1081 atgaggtcgg cgggctcgat gcccctgaac acgttgacca tgcggagccg cctgctgagc
     1141 ttcttcttct tggcctcgct cttggtcttc tggatctcct ccttgagccg tgaagacagc
     1201 tccaggatat cgagcgaacc gatgagctcg tctatggcct cggcacccat cttcgcggtg
     1261 aaactgccga tcccgtactt gtcgaccgcc tggcggaact tgtcctcggt caggagctcc
     1321 cccaacgcga gcggggtgtt cttggggtcg gtgacgatgt agctctcgaa gtagagcacc
     1381 ctctcgagtt ccttgatgga gaggtccaaa agcgcgccga tcttgctcgg caggctcttc
     1441 aggaaccaga tgtgggccac cggcgtcgcg agctcgatgt ggcccatgcg ctcgcgtctc
     1501 acccgcgagt cgatcacctc gacgccgcac ttctcgcaca ccacgccccg gtgcttcatg
     1561 cgcttgtact tcccgcagat gcactcgtag ctcttgatgg gcccgaagat gcgggcgcag
     1621 aagagaccgt cgcgctcagg cttgaaggtc cggtagttga tggtctccgg tttcttcacc
     1681 tcgccgtagg accacgcgag gatcttctcg ggcgaggcga gcccgatgcg caccgccatg
     1741 tagttggaag gatccttggg tttctcgaaa tagctgtaca ggtcttccaa tgccgtccct
     1801 ccttaatgct ggacctgccc ctctatgagg gagatatcca ggcccaggct cttgagctcc
     1861 ctggtgagaa cgttgaacga ctccggtatg ccgggttcca gctcgtgctg gcccttcacg
     1921 atggactcgt acatcctggt gcggccggcc acgtcgtcgg acttgaccgt gaggaattcc
     1981 tggagcgtgt aggccgcgcc gtaggcctcg atcgcccaca cctccatctc cccgagcctc
     2041 tggcctccga actgggcctt gccgccgagc ggctgctgcg tgacgagcga gtaggggccg
     2101 atcgagcggg cgtggatctt gtcgtcgacc aggt
//
LOCUS       DAOSCR010000491         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34306_length_2127_cov_3.158784, whole genome shotgun sequence.
ACCESSION   DAOSCR010000491 DAOSCR010000000
VERSION     DAOSCR010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34306_length_2127_cov_3.158784"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLT69_10740"
     CDS             complement(<1..748)
                     /locus_tag="PLT69_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOJ14960.1"
                     /translation="MENDRVSTGISGLDEILDGGLIPGRTYLVRGGPGKGKTTLGLHF
                     LVSGKAKGDKALFISLGEPESSVRRNAERQGLDLGGVSFLDLSPTPAFFAEVETYDIF
                     SPAEVEREPITRRIVEEVENLKPSRVFIDSMTQFRYLAADPFQYRKQVLSFLRFLVER
                     NATVLFASESSSEAPDDDLQFVCDGVIRLNDAPEGRSLSVSKFRGSSFLGGRHAMVLG
                     GRGMEVFPRLVPEAYRKEFARETIPSGIPER"
     gene            1880..>2127
                     /locus_tag="PLT69_10745"
     CDS             1880..>2127
                     /locus_tag="PLT69_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14961.1"
                     /translation="MSSAPWPFDRDGSLTDSRRTRHACCLYRAAGEGFELLKRLLVPT
                     LEHGGRCLCLCGRGIGEGIPGILSSSGLDPEGLVRSGRL"
BASE COUNT          400 a          702 c          650 g          375 t
ORIGIN      
        1 cccgctcagg gattcccgag gggatggtct cgcgggcgaa ctccttccgg taggcctccg
       61 gcaccagcct gggaaagacc tccatccccc tgccgccaag caccatggcg tggcggccgc
      121 ccaggaaaga ggaccccctg aacttgctca cgctgagact gcggccctcg ggcgcgtcat
      181 tcagccggat aaccccgtcg cagacgaact ggaggtcatc atccggcgcc tccgagctgc
      241 tttccgacgc gaagagaacc gtggcgtttc tctccactag gaaacgcagg aaggagagca
      301 cctgcttgcg gtactggaac gggtcggcag ccaggtagcg gaactgggtc atggagtcga
      361 tgaacacccg ggagggcttc aggttctcca cctcctccac gatccgcctg gtgatgggct
      421 cccgttcgac ctccgcggga gaaaagatgt cgtaggtctc cacctcggca aagaacgccg
      481 gcgtggggct caggtccagg aaactcaccc cgcccaggtc gagcccctgt cgttcggcgt
      541 tcctgcggac cgaagactcc ggctcgccaa ggctgataaa gagcgccttg tcgccttttg
      601 ccttccctga caccaagaag tgcagcccca acgtggtctt gcccttgccg ggcccgccgc
      661 ggaccagata ggtcctcccc gggatgagcc cgccgtcgag gatctcgtca agccccgaga
      721 tgccggttga aacacgatcg ttctccatga gtccgcctcc cctcagtata tctctctttc
      781 accttcctgg gtgtcttttc ttgcagatcc ttaccaaggg cagggggcga cttccgacag
      841 gaaccacggc ggccattggg caatgaacag ggaagcgccg acagccctcg tcattcccca
      901 tcataatggg ccgctctcct gttgcaagag gctgccaggc agaggcgtca atgttcgtcg
      961 aaaatctatg aaaggactgc ctcgtctgca acgcccccgg cacccctgcc gtctttgatc
     1021 cggaaaggac cctggcagaa tccggtcgtg ctcgtgggac cagggccatg cccgcaccgg
     1081 ccgctcaagc gctcggcgtc ccggaggcag acaggctgtc atcgtgcatc atgccttacg
     1141 tgggatctct ccaggcaaga cagcgttctc catgtcaagg caggtgcggc ggcaagcgta
     1201 caatctgcgt cgatgcgctg tacggggctc tcatctgtca aggctgcgaa aggggttcgc
     1261 tccttgcatc tccgcctcga ggagacaccc atgggccggt tttgccttct tacctcaacg
     1321 gaagctcgtt tatggagacc tctcccttgc aaaccatgcc caaactcatt attattattt
     1381 aaatattatc aatggagcag ggaacgaccg gggagaccgg cgtgcattgc cctacgcatt
     1441 cgccggccgc cagaggatct tctccaccca ttccacaacg acgacagggc tcaggggaga
     1501 cgacggacat gcgcagcaca cgaaccgatc gcgggtgcga cagaaaacgg ggcctgcacg
     1561 agacatcgaa ccggtttcac ccgcaagggg agacgccccg cgcacctttc acgaccgcac
     1621 cagccggcgc cccggcctgg ggtgaggggc ctgcgagatg cacgaacgtg ggcgttcctc
     1681 aggatcgtcg atgcgaagcc ctctgctgga tgggtgggcc tgcggaggca gcatggccct
     1741 caactcgggc cggggccttc acctcgcgca gaaaacacac cttcggcagg atcttaggcc
     1801 ccgtaactac cctgggttcc aggtcagcca cgccgggaac cactgggctt ccggcagcaa
     1861 ggatcgaaag gggccgggca tgagcagcgc cccgtggcct ttcgacaggg atgggtccct
     1921 cacggattcc aggaggacga ggcatgcctg ctgcctctac cgggccgccg gggaggggtt
     1981 cgagctgctc aagcgcctcc tcgtccccac cctcgagcac ggcggcaggt gcctgtgcct
     2041 gtgcggcagg gggatcgggg aagggatccc gggcatcctc tcttcatcgg ggctcgaccc
     2101 ggaagggctc gtgcgctccg gcaggct
//
LOCUS       DAOSCR010000492         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34711_length_2109_cov_2.819864, whole genome shotgun sequence.
ACCESSION   DAOSCR010000492 DAOSCR010000000
VERSION     DAOSCR010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34711_length_2109_cov_2.819864"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLT69_10750"
     CDS             <1..648
                     /locus_tag="PLT69_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14962.1"
                     /translation="TLPHDLKDAAGHLSLVLQNAPRHMDSPEFRSDMLETIGQALTRI
                     DKVISKLCAADRPEEPSFCQVSLGGFLERLLERISPRMRGIELASRIDDGLEIGTDPE
                     ILERILENILVNAVEAVDEGGRIVVGARREGEGVCLWVSDNGCGMSEEFLQERLFRPF
                     QTTKKRGTGLGLWQVKNLAEKIGAQVAVRPNPDRGVTFTVRLKTQQQGDSKPRTP"
     gene            664..2028
                     /gene="prsR"
                     /locus_tag="PLT69_10755"
     CDS             664..2028
                     /gene="prsR"
                     /locus_tag="PLT69_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HOJ14963.1"
                     /translation="MRDLILIVEDETSMAKQLKWGLGDLYEVEVAGDAPTAMDVINGR
                     DPQAVILALGLPPTPDSSEEGLRLLEEIVSRKPGLKVVVVTGNTQRENAVKAVSLGAY
                     DYHTKPVDLEELRIVIKRALYLTRLERQVSELERLVEDKAQFFGMVATSPCMLELFER
                     VRKVARTDYPVLIQGESGTGKERLARAIHALSDRSERPLVIIDCGAIPETLIESELFG
                     HEKGSFTGAHARQIGKIERAQGGTVVLDEVGELPLPLQVKLLRFIQEGTIERIGGRET
                     IRVDARVIAVTNVDLKKAVEEKRFREDLYYRLNVVPLHMPALRQRPEDIPVLARHFLE
                     VYSREIGPRFKGFSSSALDAMARHAWQGNVREMQNRIRRAVVMAEGDYIQSADLDLGE
                     EVGARAGPVRTLREARDEAEIAAIREALVRHGGNISKAALDLDVSRPTLHDLIKKHGI
                     AVTR"
BASE COUNT          416 a          656 c          753 g          284 t
ORIGIN      
        1 accctgcccc acgatctcaa ggacgcggcc ggccacctct ccctcgtcct ccagaacgcc
       61 ccccgccaca tggacagccc cgagttcagg agcgacatgc tcgagaccat cggccaggcc
      121 ctgacccgga tcgacaaggt catctcgaag ctctgcgcgg cggacaggcc ggaggagccc
      181 tccttctgcc aggtatcgct cggggggttc ctggagcgcc tgctggagag gataagcccg
      241 cgcatgaggg gcatagagct cgcctccagg atcgatgacg gcctcgagat cggcacggac
      301 ccggagatcc tggagaggat cctcgagaac atcctggtca acgcggtcga ggcggtggac
      361 gaggggggga ggatcgtcgt cggcgcccgc agggaggggg aaggggtctg cctgtgggtc
      421 tcggacaacg ggtgcggcat gagcgaggag ttcctccagg aaaggctgtt caggcctttc
      481 cagacgacca agaagcgcgg gaccgggctc ggcctgtggc aggtgaagaa cctggccgag
      541 aagatcggcg cacaggtggc cgtcaggccc aacccggaca ggggagtgac gttcacggtg
      601 aggctcaaga cgcagcagca gggggactcg aaaccccgta cgccatgagg agacgcccaa
      661 gacatgagag acctgatcct gatcgtggaa gacgagacca gcatggccaa gcagctcaag
      721 tgggggctcg gcgacctgta cgaggtggag gtggccgggg acgccccgac cgccatggac
      781 gtgatcaacg gccgggatcc gcaggccgtc atcctcgccc tgggattgcc gcccacccct
      841 gactcctcgg aagaggggct caggctcctc gaggagatcg tgagcaggaa gccgggcctc
      901 aaggtagtgg tggtcacggg caacacccag agggagaacg cggtcaaggc ggtttccctg
      961 ggcgcgtacg actaccatac caagcccgtg gacctcgagg agctcaggat agtgatcaag
     1021 cgggcactgt acctgacgag gctcgagcgc caggtgagcg agctcgagag gctggtcgag
     1081 gacaaggcgc agttcttcgg catggtggca acgtccccct gcatgctcga gctcttcgag
     1141 agggtcagga aggtggcgcg caccgactac cccgtgctca tacagggaga gagcggcacg
     1201 ggcaaggagc ggcttgccag ggcgatccat gcgctcagcg accgcagcga acggccgctg
     1261 gtgatcatcg actgcggggc catacccgag accctcatcg agagcgagct cttcggccac
     1321 gagaaggggt cgttcaccgg cgcgcacgcg cgccagatcg ggaagatcga gcgcgcccag
     1381 ggcggcacgg tggtcctcga cgaggtgggc gaactccccc tgcccctcca ggtgaagctc
     1441 ctcaggttca tccaggaggg caccatcgag cgcatcggcg gccgcgagac catccgggtg
     1501 gacgccaggg tgatcgccgt cacgaacgtg gacctgaaga aggcggtcga ggagaagagg
     1561 ttccgggagg acctctacta caggctcaac gtggtgccgc tccacatgcc cgcgctcagg
     1621 cagcggcccg aggacatccc cgtgttggct cgccacttcc tggaggtgta cagcagggag
     1681 ataggcccgc gtttcaaggg gttttccagc tccgccctcg acgccatggc gaggcacgcc
     1741 tggcagggga acgtgaggga gatgcagaac aggatcaggc gcgccgtcgt catggccgag
     1801 ggcgactaca tccagagcgc cgacctcgac ctcggcgagg aggtgggtgc ccgggccggg
     1861 cccgtgagga ccctccgcga ggcaagggac gaggcggaga tcgcggccat cagggaggcg
     1921 ctggtgcgcc acggcgggaa catctcgaag gcggccctcg accttgacgt gagcaggccc
     1981 accctgcacg acctcatcaa gaagcacggc atagcggtga cgaggtgaag gatcgcctct
     2041 tcgcccccta gagggtggtc aggcctcccg gctgtcgagg tctccagaaa cacggaagcc
     2101 gcgggcgcc
//
LOCUS       DAOSCR010000493         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34788_length_2105_cov_3.330732, whole genome shotgun sequence.
ACCESSION   DAOSCR010000493 DAOSCR010000000
VERSION     DAOSCR010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34788_length_2105_cov_3.330732"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLT69_10760"
     CDS             <1..1235
                     /locus_tag="PLT69_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ14964.1"
                     /translation="VISLMSGTFNNTLILGSVSIGFVIVATITMIRINRERAAASERN
                     KWADNVLVAHKRLETIFNGVPHYLVMVDASFTITDVNERTCDLFGLKRTDVIDKHCSL
                     DFPEGERICHTRLVERCFRTGSVVTESDTRIDIKGKPYFFDISAIPLFGSEGEVDYVV
                     QYAVDITEKKALTEKLIQAEKLAAVGQMSAHVAHEIRNPLTSIMLHSELLEDELEDIE
                     AATDAKELLAIIKSEIDRLSQVTDEYLSYARLPHPKKQLVDPMVEVSSVVAQLMPELK
                     RRNVETTITCAEKLPNIMIDRGQFKQLLINLIKNAEDAMPSGGSLEISLMGIKENVLL
                     LVKDTGYGIPPELTRRIFDPYFTTKDNGTGLGLALVQYIVNAHDGWVDVESQKGSGAT
                     FIVSFPAATPTAGQEAQS"
     gene            1232..>2105
                     /locus_tag="PLT69_10765"
     CDS             1232..>2105
                     /locus_tag="PLT69_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14965.1"
                     /translation="MRHPDTRAVALFAILVLLFWQATPAHAARVTARVPSGTIEVKLL
                     SPGVTAVLDVLRPRHEQRLKASREETGVDRLEQEVRRARQEAERARQAFENARREAKA
                     HAVSKIRVTVHKADVHISSESSIGEAEFFYTAFNESGLVVSDITYRPVVSGVVLPLTT
                     PLTLEFIDPSNLLLGLGPQETITNQNKDPERLTFFLSDLSEGDRRKVSGAAPGDFSLK
                     VEDMHFVARKGYKGQNKPMGIEEAYSSRLAPFSDAVQRAEAHRDALAQELARASQAYA
                     AQTREGTERFRAQLK"
BASE COUNT          455 a          686 c          642 g          322 t
ORIGIN      
        1 aggtcatctc cctcatgtcc gggaccttca acaacaccct catcctgggc tcggtctcca
       61 tcggcttcgt gatcgtggcg acgatcacca tgatcaggat caaccgcgaa cgggccgcgg
      121 cctcggagag gaacaagtgg gcggacaatg tgctcgtggc ccacaagcgc ctggagacca
      181 tcttcaacgg cgtaccccac tacctggtca tggtggacgc gtcgttcacc atcaccgacg
      241 tgaacgaacg gacctgcgac ctcttcggtc tcaagcgcac ggacgtgata gacaagcact
      301 gctccctcga ctttcccgag ggtgagagga tctgccacac caggctcgtg gagcgctgct
      361 tcaggacagg gtccgtcgtg accgagtctg acacgcgcat agacatcaag ggcaagccct
      421 acttcttcga cataagcgcc ataccgctct tcggttcaga gggcgaggtg gactacgtgg
      481 tccagtacgc ggtcgacatc acggagaaga aggcgttgac cgaaaagctc atacaagcgg
      541 agaagctggc ggcagtggga cagatgtcgg cgcacgtggc gcacgagatc cgcaaccccc
      601 tgacgtccat catgctccac tcggagctcc tcgaggacga gctggaggat atcgaggcgg
      661 cgacagacgc caaggaactc ctggccatca tcaagtccga gatagaccgg ctcagccagg
      721 tcacggacga gtacctctcg tacgccaggc tgcctcaccc caagaaacag ctcgtcgacc
      781 cgatggtgga ggtatcgtcg gtggtggccc agctcatgcc cgagctcaag cgcaggaacg
      841 tggagaccac gatcacctgc gccgaaaagc tcccgaacat catgatagac cgtggacaat
      901 tcaagcagct tctgataaac ctcatcaaga acgcggagga cgccatgccc tccggcggct
      961 ccctggagat atccctcatg gggatcaagg aaaacgttct cctcctcgtc aaggacacag
     1021 gttacggtat accaccggag ctcacccgca ggatcttcga cccgtacttc accacgaagg
     1081 acaacgggac aggcctgggc ctggcgctcg tccagtacat cgtgaacgca cacgacgggt
     1141 gggtcgacgt agagagccag aagggcagcg gcgccacctt catcgtatcg ttcccggcgg
     1201 cgacgcccac cgcgggccag gaggcacagt cgtgaggcac ccggacacac gcgccgtagc
     1261 gctcttcgcg atcctcgtcc tcctcttctg gcaggcgacc cccgcacatg cggcacgcgt
     1321 gaccgcacgg gtcccctcgg ggaccatcga ggtcaagctc ctctcccccg gtgtgaccgc
     1381 ggtgctcgac gtgctcaggc ccaggcacga gcagcgcctc aaggcctcac gggaggagac
     1441 cggggtcgac aggctcgagc aggaggtgcg tcgggcacgc caggaggccg agcgggcacg
     1501 ccaggcattc gagaacgcca ggagggaagc aaaggcccac gcggtctcga agatccgcgt
     1561 caccgtgcac aaggccgatg tccacatctc ttcggagtcg tcgatcggcg aggccgagtt
     1621 cttctacacg gcgttcaacg agtcgggcct cgtcgtgagc gacatcacgt acaggccggt
     1681 cgtctcgggg gtggtcctcc cgctgacgac ccccctgacc ctcgagttca tcgacccatc
     1741 gaacctcctc ctcgggctcg ggcctcagga gaccatcacc aaccaaaaca aggatcccga
     1801 gaggctgacc ttcttcctga gcgacctcag cgagggcgac aggcgaaagg tatcgggggc
     1861 ggcacccggg gacttttccc tcaaggtgga agacatgcac ttcgtcgcca ggaagggata
     1921 caagggccag aacaagccca tgggcataga ggaggcctac tcctcccgat tggccccctt
     1981 cagcgacgcc gtgcaacgtg ccgaggcgca cagggatgca ttggcccagg agctcgccag
     2041 ggccagccag gcatacgccg cccagacgag ggaaggcacg gagagattca gggcgcaact
     2101 gaagg
//
LOCUS       DAOSCR010000494         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34860_length_2102_cov_3.898876, whole genome shotgun sequence.
ACCESSION   DAOSCR010000494 DAOSCR010000000
VERSION     DAOSCR010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34860_length_2102_cov_3.898876"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="PLT69_10770"
     CDS             complement(<1..2084)
                     /locus_tag="PLT69_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOJ14966.1"
                     /translation="MTELYRKEGYASPKVRVKVERQEGATVVIGVHIEPGRYYTLESI
                     KIRGNEALLDAEILSRMRSWRHSLFIRESGRFREEELARDVADLVAVYRQRGYPECTI
                     DLELLRDASSRSVQALLKVSEGPKYEVSITGNSHFWDYTLRKDIVIFDEGNIGNRGLR
                     KSVRNMLARYRAQGYLQARIDILEERQTAGRRRVRSIEFAVTEGPRTLVEKVDFPGAT
                     AFREDDLREMVETGRPGLFHAPVYDPDLLERDIQALRAFYLKNGYTQVSIEPKTSFRD
                     DRTAVSISFHIAEGPRTVVSSIDIKGLTVTPPSRVEASLSLKQGGPFVEAVLKADEAL
                     ISELVSARGRPYVKVSPRVTMSEDRTSAKVEFEVDEGPEVVMGAIYFQGNFTTRTSVI
                     RRELKMEPGQLFSLRGMLEGQKRVRDMQAFNSVQFRTLGIRERLERVTLLVDVQESEP
                     YYYQGGLGYVTDRGVYAHIKAGDRNLFGLNKHLWTGGEVSQTGYKAETGLTQQRIFDA
                     AVTNTSTLSYEHEEAFNQIFGTRTLSASTIFSWKPTSHLSTSIQGRYEYRDQFLQEGS
                     ASIPEGDEDAYEPRGVLVATPGIVRDTRDSFVRPTRGSYSSYSLDVSRGFRSSLDNFL
                     RHTVNLRLYYGPHERLTFAWMGRYTYLDPYGKVSRIPKDQLAYLGGTMSVRGFDENML
                     RYDSKGDPVGGRL"
BASE COUNT          326 a          696 c          643 g          437 t
ORIGIN      
        1 aggcggcctc ctaccgggtc tcccttcgag tcgtagcgga gcatgttctc gtcgaacccg
       61 cgcacgctca tggtccctcc caggtaggcg agctggtcct tggggatccg gctgactttc
      121 ccgtagggat cgaggtaggt gtacctcccc atccaggcga aggtgagtct ctcgtgaggg
      181 ccgtagtaga gcctcaggtt caccgtgtgc ctgaggaaat tgtccaggga ggacctgaaa
      241 cccctggaga cgtcaagcga gtaggaggaa tacgagcccc tcgtcggcct cacgaacgag
      301 tccctcgtgt ccctgacgat ccccggggtc gccacgagga cgcccctggg ctcgtaggca
      361 tcctcatcac cctccgggat ggaggcggag ccttcctgga ggaactggtc cctgtactcg
      421 tagcgtccct ggatggaggt ggagaggtgg gaggtgggct tccaggagaa gatcgtcgac
      481 gccgacagcg tcctggtgcc gaagatctgg ttgaacgcct cttcgtgttc gtaggacagc
      541 gtggaggtgt tcgtcacggc tgcgtcgaag atgcgctgct gggtgaggcc cgtctccgcc
      601 ttgtagcccg tctggctcac ctcgccccct gtccacaggt gcttgttcag gccgaagagg
      661 ttccggtcgc ccgccttgat gtgtgcgtag acgcccctgt ccgtgacgta gcccagaccc
      721 ccctggtagt agtagggctc ggactcctgc acgtcgacga ggagcgtcac ccgctcgagg
      781 cgctccctga tgcccagggt cctgaactgc accgagttga aggcctgcat gtcgcggacc
      841 ctcttctgtc cctcgagcat gcccctgagg gagaacagct ggccgggctc catcttcagc
      901 tccctgcgta tcacgctcgt gcgcgtggtg aagttgccct ggaaatagat cgcccccatg
      961 acgacctccg gcccctcgtc cacctcgaac tcgactttgg ccgatgtcct gtcctcggac
     1021 atggtcaccc gcgggctcac cttgacgtag gggcggcccc tggcggagac cagttccgag
     1081 atgagcgcct cgtcggcctt gagcaccgcc tccacgaacg gcccgccctg cttgaggctc
     1141 aaggaagcct cgaccctgga cgggggcgtc acggtgaggc ccttgatgtc gatggaggac
     1201 accacggtcc tcggcccctc ggcgatgtgg aaggatatcg agacggccgt ccggtcatcc
     1261 ctgaaggagg tcttgggctc tatggaaacc tgggtgtacc cgttcttcag gtagaacgcg
     1321 cggagggcct gtatgtcgcg ctcgaggagg tcggggtcgt acacgggggc atggaacagc
     1381 cccggccgtc ccgtctccac catctccctg agatcgtcct ccctgaacgc cgtcgcgccc
     1441 gggaagtcga ctttttccac cagcgtcctc gggccctcgg tgacggcgaa ctcgatggaa
     1501 cgcacccggc gcctgcctgc cgtctggcgt tcctcgagga tgtctatcct cgcctgcagg
     1561 tacccctggg ccctgtagcg cgcgagcatg ttcctcacgc tcttgcggag ccccctgttc
     1621 ccgatgttcc cctcgtcgaa gatcacgatg tcctttctca gggtgtaatc ccagaaatgg
     1681 ctgttgccgg tgatggacac ctcgtacttc ggcccctcgg acaccttgag cagggcctgc
     1741 accgaacggc tggacgcgtc ccttaggagt tccaggtcta tggtgcactc ggggtatccc
     1801 ctctggcggt acacggccac gaggtccgcc acgtccctgg cgagttcttc ctccctgaac
     1861 ctccctgatt ccctgatgaa caacgaatgc ctccacgagc gcatgcgcga gagtatctcc
     1921 gcgtcgagga gcgcctcgtt gcccctgatc ttgatcgatt ccagggtgta gtacctgccg
     1981 ggttctatgt ggacgccgat caccacggtg gcgccctcct gtctttccac cttcaccctg
     2041 acctttgggc tcgcgtaccc ctccttcctg tagagctcgg tcaagagttt ttcctgttcc
     2101 tc
//
LOCUS       DAOSCR010000495         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_34890_length_2101_cov_3.295210, whole genome shotgun sequence.
ACCESSION   DAOSCR010000495 DAOSCR010000000
VERSION     DAOSCR010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34890_length_2101_cov_3.295210"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLT69_10775"
     CDS             <1..656
                     /locus_tag="PLT69_10775"
                     /inference="COORDINATES: protein motif:HMM:NF037782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14967.1"
                     /translation="PMSQRGQVMRLIERESGLEDILLRPLCARLLPPTAPERAGIVDR
                     ASLGGIRGRGRKVQMEMARELGIAEHDMPSPAGGCLLTDEQIARKAKITFDSLSPALP
                     SRADLLLDVVGRRFRLKEKTVLVVGRNEQENALIARFVHPGNVFMRMRDVPSPLCILR
                     GEVDASCLETAAAVCLRYTKARGEPGHVVVHGERPDDLANEVAAPVVDEEFVRKLQD"
     gene            692..889
                     /locus_tag="PLT69_10780"
     CDS             692..889
                     /locus_tag="PLT69_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOJ14968.1"
                     /translation="MGYFLSVLGMVFIVEAVPYILFPAKVKSFARSVQDIKDGTLQAA
                     GLVAALVGLLVIALGRGMTGM"
     gene            895..1902
                     /gene="queA"
                     /locus_tag="PLT69_10785"
     CDS             895..1902
                     /gene="queA"
                     /locus_tag="PLT69_10785"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ14969.1"
                     /translation="METSRYFYDLPRELVAKYPQPRGTDRLLVVSRADSSISHRRFSD
                     LPGFLRPTDVLVLNDTRVIPARLEGRKATGGAVEVLLLKELEPCRWLSLVRASKPIKP
                     KTMVFFDGGHWVRVEEGSGGKYVVTLSRPSLVQDVGKVPLPPYMEREPEDIDREAYQT
                     VYASKDGSVAAPTAGLHFTRDMLEEIAVLGVETAYVTLHVGVGTFAPIRTARVEDHVM
                     HEEEYDVSADSAATIARALEEGGRIVAVGTTVTRVLEHLMLEHGRITAASGTTSLFIT
                     EGFPFRATSALLTNFHLPCSTLLVLVCAFGGYELVMRAYREAVERRYRFFSYGDAMLI
                     V"
     gene            1877..>2101
                     /locus_tag="PLT69_10790"
     CDS             1877..>2101
                     /locus_tag="PLT69_10790"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOJ14970.1"
                     /translation="MATRCSSFEFKVTHTDGGARAGLMRTRRGVVPTPAFMPVGTAAT
                     VKAVTPEQLKALGARIILGNTYHLHLRPGEE"
BASE COUNT          359 a          660 c          751 g          331 t
ORIGIN      
        1 gccccatgtc ccagaggggc caggtcatgc gcctcatcga gagggaatcc gggctcgagg
       61 acatcctcct gaggccgctt tgcgcccgcc ttctcccccc gaccgccccg gagcgggcgg
      121 ggatcgtgga cagggcgtcg ctgggcggca tcaggggcag ggggaggaag gtccagatgg
      181 agatggcccg agagctcggg atcgcggaac acgacatgcc cagccccgcc ggcggctgcc
      241 tcctcacgga cgagcagatc gcgcgcaagg cgaagatcac gttcgattcc ctgtcccccg
      301 ccctcccatc cagggcggac ctccttctcg acgtcgtcgg ccggaggttc aggctcaagg
      361 agaagacggt gctcgtcgtg gggaggaacg agcaggaaaa cgcgctgata gccaggttcg
      421 tccacccggg caacgtgttc atgaggatga gggacgtgcc ctcgcccctg tgcatcctca
      481 ggggtgaggt ggacgcctcg tgcctggaga cggccgcggc cgtctgtctc aggtacacga
      541 aggcgagagg cgagccgggt cacgtggttg tccacgggga gcggccggac gacctcgcga
      601 acgaggtggc ggcgcccgtc gtcgacgagg agttcgtcag gaagctccag gattgacgcg
      661 cagacaaggc gttggctata agagcggagc tatgggctac tttctgagcg tcctcgggat
      721 ggtcttcatc gtggaggccg tcccgtacat actcttcccg gcgaaggtga agtccttcgc
      781 ccgctcggtg caggacatca aggacgggac cctccaggcg gccggtctcg ttgcggccct
      841 cgtgggtctg ctcgtgatcg ccctcggcag gggcatgacg gggatgtgac ccgggtggag
      901 acgtcgcggt atttctacga cctgcccagg gagctcgtcg cgaagtaccc gcagccgagg
      961 gggacggacc gcctcctcgt ggtgagccgt gccgactcga gcatcagcca ccgccggttc
     1021 tcggatctcc cgggcttctt gaggccgacg gacgtgctcg ttctcaacga cacgagggtg
     1081 atcccggcga ggctggaagg gaggaaggcg acgggcggcg cggtggaggt cctgctcttg
     1141 aaggagctgg agccctgcag gtggctgagc ctggtgaggg cctccaagcc cataaaaccg
     1201 aagacgatgg tcttcttcga cggcggccac tgggtgcgcg tagaggaggg gtcgggaggg
     1261 aagtacgtgg tgacgctgag ccggccgtcc ttggtgcagg acgtggggaa ggtcccgctg
     1321 ccgccctaca tggaacgtga gcccgaggat atcgacaggg aggcctacca gaccgtgtat
     1381 gcaagcaagg acgggtcggt cgccgcgccc acggccggcc ttcacttcac gcgcgacatg
     1441 ctggaggaga tagccgtcct cggcgtcgag accgcctacg tgaccctcca cgtgggggtg
     1501 gggaccttcg cacccatccg taccgcaagg gtcgaggatc acgtcatgca cgaagaggag
     1561 tacgatgtca gcgccgactc ggccgcgacc atcgcccggg cgctcgagga ggggggccgc
     1621 atagtcgccg tggggaccac ggtcaccagg gtgctcgagc acctcatgct cgaacatggc
     1681 cgcatcacgg ccgcctccgg gacgacgagc ctcttcataa ccgaggggtt cccgttcagg
     1741 gcgacctccg ccctgctcac gaactttcac ctcccgtgct cgacgctgct cgtcctcgtg
     1801 tgcgccttcg gcgggtacga gctcgtcatg agggcctaca gggaggcggt ggaaaggcgc
     1861 tacaggttct tcagctatgg cgacgcgatg ctcatcgttt gaattcaagg tgacccacac
     1921 cgacgggggc gcaagggcgg gcctcatgcg cacgcgcagg ggggtggtgc cgacgcccgc
     1981 cttcatgccc gtggggaccg cggcgacggt caaggccgtg acgccggagc agctcaaggc
     2041 gctgggcgca cgtatcatcc tcggcaacac ctaccacctc cacctgaggc cgggcgagga
     2101 a
//
LOCUS       DAOSCR010000496         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35330_length_2083_cov_3.090237, whole genome shotgun sequence.
ACCESSION   DAOSCR010000496 DAOSCR010000000
VERSION     DAOSCR010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35330_length_2083_cov_3.090237"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLT69_10795"
     CDS             <1..617
                     /locus_tag="PLT69_10795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847361.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOJ14971.1"
                     /translation="RAAKLSDALGGGSLTALPVVETQAGDVSAYIPTNVISITDGQIF
                     LDTDLFYSGFRPAINVGISVSRVGGNAQIKAMKKVAGSLRLDLAQYRELAAFAQFGSD
                     LDKATQAQLTRGERLMELLKQPQYQPLPVEKQVAIIYVGTQGHLDEYPVSAVKKFEEQ
                     FYTFLESKHPEILKTIRETGKLEADTEKALKDAIAEFKKIFVVE"
     gene            636..1496
                     /gene="atpG"
                     /locus_tag="PLT69_10800"
     CDS             636..1496
                     /gene="atpG"
                     /locus_tag="PLT69_10800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOJ14972.1"
                     /translation="MPSLKDLRKRINSVKKTRQITSAMRMVAAAKLRRSQSDIIAARP
                     YAIKTNEVLVSLVTRTNPDMHPLLRVREPKRGVVVVIASDRGLCGSFNQNLFRKAETF
                     IKQNRDRYEDIQMVLIGKRAGDYFKRKNVAKRAVYTIGTPSYELASEICDDLIKGYVN
                     AEYDELTIIFNEFRSALTQVMHVDRVLPVEPMEAEDDMMNVEYLYEPSEDILLEALLP
                     LSLKVFMYRALLESAASEHGARMTAMESASKNAGEVIGKLEIKYNRARQSAITTELME
                     VVGGAEALKG"
     gene            1530..>1747
                     /locus_tag="PLT69_10805"
     CDS             1530..>1747
                     /locus_tag="PLT69_10805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237076.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ14973.1"
                     /translation="MNIGKISQIIGAVVDVEFPQGELPNIYNALLVTSPFLSDEEDNL
                     VLEVAQHLGNRTVRTIAMDSTDGLARGMP"
     assembly_gap    1748..1757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1758..>2083
                     /locus_tag="PLT69_10810"
     CDS             <1758..>2083
                     /locus_tag="PLT69_10810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237076.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ14974.1"
                     /translation="KDTGAPISVPVGNAVLGRILNVVGKPVDEAGPVNTDKFYPIHRE
                     PPKFIEQSTSIESFETGIKVIDLLTPYMRGGKIGLFGGAGVGKTVFIMELINNIAKKH
                     GGFSVFS"
BASE COUNT          450 a          652 c          643 g          328 t
ORIGIN      
        1 agcgcgcggc caagctgagc gacgcgctcg gaggcgggtc gctcacggcc ctgccggtgg
       61 tcgagacgca ggcaggcgac gtgtccgcct acatcccgac gaacgtcatc tccattaccg
      121 acggccagat attcctcgac accgacctct tctactccgg cttcaggccc gccatcaacg
      181 tgggcatctc ggtgtcccgc gtgggcggca acgcccagat caaggcgatg aagaaggtcg
      241 ccggctccct gaggctcgac ctggcccagt accgcgagct cgccgcgttc gcgcagttcg
      301 gttcggacct cgacaaggcg acgcaggccc agctcaccag gggcgagcgc ctcatggaac
      361 tactcaagca gccgcagtat cagccgcttc cggtcgagaa gcaggttgcg atcatctacg
      421 tgggcaccca gggccacctc gacgagtatc ccgtctcggc cgtgaagaag ttcgaggaac
      481 agttctatac cttccttgaa agcaagcacc ccgagatcct caagacgatc cgggagaccg
      541 gcaagctcga agccgacacc gagaaggcac tgaaggacgc catcgcagag ttcaagaaga
      601 tcttcgtggt ggaataaaga cggttaggag ctcacatgcc aagtctcaag gacttacgca
      661 agaggatcaa ctcggtcaag aagacgaggc agatcacgtc ggcgatgcgg atggtggccg
      721 cggcgaagct ccgcaggtcg cagagcgaca tcatcgccgc acggccgtat gccataaaga
      781 ccaacgaggt cctcgtgtcg ctcgtcacga ggacgaaccc cgacatgcac cccctcctca
      841 gggtgcgtga gcccaagcgc ggggtggtgg tggtgatcgc ctccgacagg gggctgtgcg
      901 ggtcgttcaa ccagaacctc ttccgcaagg ccgagacctt catcaagcag aaccgcgacc
      961 ggtacgagga catccagatg gtgctcatcg gcaagcgtgc cggcgactac ttcaagcgca
     1021 agaacgtggc gaagcgcgcg gtctacacga tcggcacgcc gagctacgag ctcgcgagcg
     1081 agatctgcga cgacctcatc aagggctacg tgaacgccga gtacgacgag ctcaccatca
     1141 tcttcaacga gttccgctcg gctctgaccc aggtcatgca cgttgacagg gtgctgcccg
     1201 tggagcccat ggaggccgag gacgacatga tgaacgtcga gtacctctac gagccgtccg
     1261 aggacatcct gcttgaggcg ctgctgccgc tcagcctcaa ggtgttcatg taccgggcgt
     1321 tgctcgagtc cgcggcatcc gagcacggcg cgaggatgac cgccatggag agcgcctcca
     1381 agaacgcggg cgaggtcatc gggaagctgg agatcaagta caacagggcc cgccagtcgg
     1441 ccatcaccac cgagctcatg gaggtggtgg gcggtgccga ggcactgaag ggataacaac
     1501 cttggatcct aatcgggagg attgatcgca tgaatattgg caagatctca cagatcatcg
     1561 gcgcggtcgt ggacgttgaa ttcccccagg gagagctgcc gaacatatat aacgccctgc
     1621 tggtgacgag ccccttcttg tccgacgagg aggacaacct ggtgctcgag gtcgcccagc
     1681 acctggggaa ccgcacggtc cgcaccatcg ccatggactc caccgacggc ctggcgcgcg
     1741 gcatgccnnn nnnnnnnaag gataccgggg cgcccatatc ggtgcccgtg ggcaacgccg
     1801 tgctgggcag gatcctcaac gtggtgggca aacccgtgga cgaggcaggc ccggtcaata
     1861 ccgacaagtt ctaccccatc caccgcgagc cccccaagtt catcgagcag agcacgtcga
     1921 tcgagtcgtt cgagacgggc atcaaggtca tcgacctcct caccccctac atgagaggcg
     1981 gaaagatcgg cctgttcggg ggcgcgggcg taggcaagac ggtgttcatc atggagctca
     2041 tcaacaacat cgccaagaag cacggcggct tctccgtgtt ctc
//
LOCUS       DAOSCR010000497         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35402_length_2080_cov_2.973827, whole genome shotgun sequence.
ACCESSION   DAOSCR010000497 DAOSCR010000000
VERSION     DAOSCR010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35402_length_2080_cov_2.973827"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PLT69_10815"
     CDS             <1..1079
                     /locus_tag="PLT69_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOJ14975.1"
                     /translation="PAGSHKPNTAVPQAWYNKQFGIERLTTETGAGQWGSALSYACAL
                     VGLKCKVFMVRISFEQKPFRKLMMNVWGAECVPSPSTETKTGRDILEHDPDTPGSLGI
                     AISEAVETAVADTTGKTRYSLGSVLNHVLLHQTIIGLEAKKQLAKIGEKKVDVVIGCA
                     GGGSNFAGLSFPFVLDKINGAQIDIIPVEPTSCPTLTRAPFAYDHGDVAKMTPLLPMH
                     SLGHEFVSPPIHAGGLRYHGMAPLVSAAVVEGLLTPKAMNQLKCYESALFWARTEGFI
                     PAPETSHAIAAVIDEAVKAREEGKEKVILFNWSGHGLMDLKGYESYMEGRLTDYPLPE
                     EDLQKSLKSIENLPKPKVAKTGRW"
     gene            1073..1339
                     /locus_tag="PLT69_10820"
     CDS             1073..1339
                     /locus_tag="PLT69_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14976.1"
                     /translation="MVRIASSPYEDLETGCPMLGHPVPLRYCLEPGSELPCRRIVLCW
                     QERFDIAGLLREVLTPEQLEELEKAPKQKISQLLELVAKVKKGS"
     gene            complement(1627..>2080)
                     /locus_tag="PLT69_10825"
     CDS             complement(1627..>2080)
                     /locus_tag="PLT69_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ14977.1"
                     /translation="LARAGAPVPTRTAYTAGLLHDIGKVVLDQYMARIQPLFYRVLKA
                     HGEDSSALERSIFGIDHNLAGLHLSEAWGLPASIREAILFHHDPAHATAHEELVHLVH
                     VADTLSLRFLPGLVIENPDPRPIDESMRILGLGRDAVEGALVALRRLY"
BASE COUNT          372 a          662 c          720 g          326 t
ORIGIN      
        1 caccggcggg cagccacaag cccaacacgg ccgttccgca ggcctggtac aacaagcagt
       61 tcggcatcga gcggctgacc acggagaccg gggcgggcca gtgggggagc gccctctcgt
      121 acgcatgcgc gctcgtgggg ttgaagtgca aggtcttcat ggtcaggatc agcttcgaac
      181 agaagccttt ccggaaactc atgatgaacg tgtggggcgc cgagtgcgtg ccgagcccgt
      241 ccactgagac gaagacgggc agggatatcc tcgagcacga cccggacaca ccgggcagcc
      301 tggggatcgc catcagcgag gcggtggaga cggcggtcgc ggacacgacg ggaaagacca
      361 ggtactcgct gggcagcgtc ctgaaccacg tactcctcca ccagacgatc atagggctcg
      421 aggcgaagaa gcagctcgcc aagatcggcg agaagaaggt cgacgtggtc atcgggtgcg
      481 cgggcggcgg gagcaacttc gcaggcctct cgttcccctt cgtgctcgac aagatcaacg
      541 gcgcccagat agacatcatc ccggtcgagc ccacctcgtg cccgaccctc acgagggcgc
      601 cgttcgccta cgaccacggg gatgtggcca agatgacgcc gctgttgccc atgcattccc
      661 tcggccacga gttcgtctcc ccgcccatcc acgcgggcgg cctcaggtat cacggcatgg
      721 cgccgctggt gagtgcggcg gtggtggagg gactgctcac gcccaaggcc atgaaccagc
      781 tcaagtgcta cgagagcgcc ctgttctggg cacgcaccga gggcttcatc cccgccccgg
      841 agacctcgca cgccatcgcg gccgtcatcg acgaggcggt caaggcgagg gaggaaggca
      901 aggagaaggt catcctcttc aactggagcg ggcacggcct catggacctc aagggctacg
      961 agagctacat ggagggacgg ctcacggact acccgctgcc cgaggaggat ctccagaagt
     1021 ccctgaagtc catcgagaac ctgccgaagc ccaaggtggc gaagaccggg agatggtgag
     1081 gatcgcgtcc tctccgtacg aagacttgga gaccgggtgc ccgatgctgg ggcacccggt
     1141 gcctctcagg tactgcctcg agccggggtc cgagctcccg tgccggagga tcgtcttgtg
     1201 ctggcaggaa cgcttcgaca tcgcgggcct cctgagggag gtcctcacgc ccgagcagct
     1261 cgaggagctc gagaaggcgc cgaagcagaa gatctcccag ctcctcgagc tggtggcaaa
     1321 ggtgaagaag gggtcttgac ggtcttctcc gcgggtcccc tgtccccggc gatgccctgt
     1381 cgccgtccat acccccgggc agggttcacc ggacgatcta ggccgtgacg cccacgttca
     1441 ggagcgggcc cggctctcgt cccttgtctc gggggctcct cctgcgcgcg gatgcgtgca
     1501 tgcggacccc ttcgacggca tgcaaggcag ctccatgggg ccggaccgcc tcggtcgatt
     1561 gcggggtggt gtcgcctcga tgacaaggcg acaccgctag ccgccgtctc gtgatcgtcg
     1621 accctctcag tagagccttc tcaaggcgac gagggcgccc tccacggcat ccctgcccag
     1681 gcccaggatg cgcatgctct cgtcgatcgg cctggggtcg gggttctcga tcacgaggcc
     1741 ggggaggaac ctcaggctca gcgtgtccgc gacgtggacg agatggacca gttcttcatg
     1801 agccgtcgcg tgagctgggt cgtggtggaa gaggatcgcc tccctgatgg acgcgggcag
     1861 gccccaggcc tcggagaggt ggagccctgc caagttgtgg tcgatgccga agatggaacg
     1921 ttcgagagcg ctcgagtctt cgccgtgggc cttgaggacg cggtagaaca ggggttggat
     1981 gcgggccatg tactggtcga gcaccacctt cccgatgtcg tggagcagcc cggcggtata
     2041 ggccgtccgg gtcgggacgg gggcgcccgc cctggcgagc
//
LOCUS       DAOSCR010000498         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35422_length_2079_cov_3.250988, whole genome shotgun sequence.
ACCESSION   DAOSCR010000498 DAOSCR010000000
VERSION     DAOSCR010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35422_length_2079_cov_3.250988"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLT69_10830"
     CDS             complement(<1..321)
                     /locus_tag="PLT69_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ14978.1"
                     /translation="MTRRAGDSRIREGGYAEVLTLALPLILSTSSWAVQHFVDRMFLA
                     WYSPQAIAAAMPAGMLNFTLMSFFVGTAGYVSTFVAQYDGAGMHHRVGPVTWQGLYVS
                     VVGGL"
     gene            548..781
                     /locus_tag="PLT69_10835"
     CDS             548..781
                     /locus_tag="PLT69_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOJ14979.1"
                     /translation="MAVHYEQVEQALEKTRGALRRDGGDVELVDVSPDGIVQVRLKGA
                     CSGCPMATLTIKSLIERVLLKEVPGVREVRAVP"
     gene            944..1537
                     /locus_tag="PLT69_10840"
     CDS             944..1537
                     /locus_tag="PLT69_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ14980.1"
                     /translation="MAASEAGNPDTLGVHTLAVPLPGFEDLGCSNAYVLGTGPVTLID
                     TAPKFPGSLFTLERQLASLGYSWSDVERIILTHGHIDHFGLVGSIREASSRRISCHIH
                     GEDAWRLSPGHLSSGMFTEEAVRFYEQAGIPAHVLDRMRRRFVFFRALCDPIEDILPM
                     SDGDTFSGLGFELRVIHTPGHSPGCCCLYEPHRKILF"
     gene            1583..1951
                     /locus_tag="PLT69_10845"
     CDS             1583..1951
                     /locus_tag="PLT69_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14981.1"
                     /translation="MNRSLLADPSYKSLEAYLSSLGRVLGLDVNLALPGHGPPIEDIQ
                     GLIEGYREHHRARTEEVARALGKGPKTVYELSFDVFPSLNESEVSLAVSEIVVHLEPL
                     LESGRARIVDTGPPILYAGS"
BASE COUNT          406 a          690 c          637 g          346 t
ORIGIN      
        1 caagccgcca accaccgaga cgtacaaccc ctgccatgtc acgggcccca ccctgtggtg
       61 catgccggcg ccgtcgtact gggcgacgaa cgtgctcacg tacccggcgg tgccgacgaa
      121 gaagctcatc agcgtgaagt tcagcatgcc cgcgggcatg gccgccgcta tcgcctgcgg
      181 cgagtaccat gcgaggaaca tcctgtccac gaagtgctgg actgcccatg aagaggtgct
      241 gagaatgagg ggaagggcaa gggtcagcac ttccgcgtac ccgccttccc ttatcctcga
      301 gtcgcccgca cggcgagtca ttcatcaccg cccatgtgtg tttgggtgaa ccatgcatgc
      361 atatcacggc atgacggccc ccagtcaagc ccgtccatgg gaaaagcccc gggcgcaccc
      421 atgcatggca ggcacgtatc gggttttccg caaagacgcc ttgccatgag agagaggtat
      481 cgtctaatga agagtgagcg aagcatcgaa cagcacgatg caggcttgca cgaaaggggg
      541 acgagccatg gcggtgcatt acgaacaggt ggaacaggca ctcgaaaaga cacgcggcgc
      601 cctgaggaga gacggcggcg acgtggagct cgtggacgtc tcgccggacg ggatcgtcca
      661 ggtgaggctc aagggtgcct gcagcgggtg tcccatggcc accttaacca taaagtccct
      721 gatagagcgc gtgctcctca aggaggtccc gggcgtcagg gaagtcaggg ccgtcccatg
      781 aacgggggct gccgacaaca agaccgcacc ttggcgctct gcccggcagc cctcccggtc
      841 acgatccacg ccctgccggc aggctgtgcc ctggccgcgc cggcaggaac tccggcccca
      901 tgccgcgccc cggccggtga cggtcaggcc ctggatggac ctcatggccg caagcgaagc
      961 cggcaatcct gacaccttgg gcgtgcacac gctcgcggta cccctgcccg ggttcgagga
     1021 cctcgggtgt tccaacgcgt atgtcctcgg taccggcccg gtcaccctca tcgacactgc
     1081 ccccaagttc cccggctctc tgttcacctt ggagaggcag ctggcctctc tcggatactc
     1141 gtggtccgac gtggagcgca tcatactgac gcacggccac atagaccatt tcggcctcgt
     1201 tggttccatc cgcgaggcct cgtcgcgcag gatttcctgc cacatccacg gcgaagacgc
     1261 ttggaggctg tcacccggcc acctctcctc gggcatgttc acggaagagg cggtgcgctt
     1321 ctacgagcag gcaggcatcc ccgcccatgt ccttgaccgc atgaggagac ggttcgtctt
     1381 cttccgcgcc ttgtgcgacc ccatagaaga catcctgccc atgtccgacg gcgacacctt
     1441 cagcgggctg gggttcgagc tcagggtgat ccacacccca ggccactcgc ccgggtgttg
     1501 ctgcctctac gaaccgcaca gaaagatcct cttctgaggg gaccacctcc tcaagcacat
     1561 aacacccaac ccattccacg aggtgaatcg ttctctcctc gcagaccctt cctacaagag
     1621 cctcgaggcg tacctgtcct cgctcgggcg ggtcttaggc ctggatgtca acctcgccct
     1681 gccgggccac gggccgccca tagaagacat acaaggactg atagaaggct atcgggaaca
     1741 ccaccgggcc cggacggagg aagtggcaag ggcgctcggg aaagggccga aaaccgtcta
     1801 cgagctgtcg ttcgacgtgt tcccgtcact gaacgaaagc gaggtatcgc tcgcggtctc
     1861 ggagatcgtg gtgcacctcg agccactcct ggagtcggga cgggcacgca tcgtggatac
     1921 gggcccccca atcctctacg cagggtcctg acggaacgcg ccccgatgga gcagtagacg
     1981 gcgccgttgt cgcgggagcc ggtcggccgt aaacgcaggc gcttgacaaa ccggaccctg
     2041 ccgttcaaag acgtgggtac cgggagatac cggtcttat
//
LOCUS       DAOSCR010000499         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35430_length_2079_cov_2.679348, whole genome shotgun sequence.
ACCESSION   DAOSCR010000499 DAOSCR010000000
VERSION     DAOSCR010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35430_length_2079_cov_2.679348"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /gene="glpA"
                     /locus_tag="PLT69_10850"
     CDS             <1..1497
                     /gene="glpA"
                     /locus_tag="PLT69_10850"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HOJ14982.1"
                     /translation="VIIIGGGATGCGIARDLALRGVPHYLVERGDFASGATGACHGLL
                     HSGARYAVRDKEAARECITENAILKTIAKKCVEVTGGLFVRLPGDSKRYRDEFLKSCE
                     ATGIETVILSPSKALELVPSLNPALEEAVRVPDCSIDPFRLCLLNATTSRRLGGNILT
                     HHEVVEIIREKGRCVGIRARERLTGEIKEIRGRIVVNASGAWGDRVARMAGSRVPMAL
                     SKGSLVITNHRLTNVVINRLRPPSDGDIIVPNEAVCLAGTTSIPVDSPDNLEIDPSEV
                     DTVTTQAEQMVPAFGTTRLIRAYTGVRPLLKASGTEADGRSISRGFQIIDHKNGMISI
                     LGGKLTTYRLMAEKTVDVVMDVLGFKVPCRTAELPLEGQDELSGYPLSKRLSKLDDIV
                     CECELVSRSDVQRAVNAMATPYVGDIQHRTRLGMGPCQGGFCTYRALGIMQEMGRVDP
                     DVSLSILKEFLQRRFKGIRPALWGDQLREEQLIESIYMGILAMGQGHG"
     gene            1490..>2079
                     /locus_tag="PLT69_10855"
     CDS             1490..>2079
                     /locus_tag="PLT69_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ14983.1"
                     /translation="MGEHGYDVVIIGGGVAGLTASIYLAGKGFRTALVSRGDPACCLS
                     TGCIDVLGAHEDPMKGIDRLPRNHPYRIAGRRTVIDALEAFLGLMEERGLPYRGSLGR
                     NTGILTPIGTTKTTCLVPITMEASSGFTTTHIHVVSFKGLRDFYPSYITSRLANTSVS
                     VFDAGTSSTLGLATKFDEPSFVDDFCAWLSGLELPPG"
BASE COUNT          401 a          675 c          671 g          332 t
ORIGIN      
        1 gtgatcatca tcggtggtgg tgctacgggc tgcggcatcg cgagggatct cgccctcagg
       61 ggtgttcccc actatctcgt ggaaagagga gacttcgcat cgggagcaac aggcgcctgc
      121 cacggcctgc tccacagcgg cgccaggtac gctgtccggg acaaggaggc cgcccgtgaa
      181 tgcatcaccg aaaacgccat cctgaaaacc atcgccaaga agtgcgtgga agtcaccggc
      241 ggtctcttcg tgaggcttcc cggagactcc aagcgctacc gagacgagtt tctgaagagc
      301 tgcgaggcca cggggatcga gactgtgatc ctgtcgccct ccaaggccct cgagctcgtc
      361 ccctcgttga accccgccct cgaggaggcc gtcagagtgc cagactgctc catcgacccc
      421 ttcaggctgt gcctgctcaa cgcaacgaca tcccgcaggc tgggcggcaa catcctcacc
      481 caccacgagg tggtggagat catccgggag aaggggcggt gcgtgggcat cagggccagg
      541 gagcggctca ccggcgagat caaggagatc aggggcagga tcgtcgtgaa cgcgagcggc
      601 gcctgggggg acagggtagc gaggatggcg gggtcgcgcg tccccatggc cctgtccaag
      661 gggagcctgg tgatcacgaa ccacaggctc acgaacgtcg tcatcaaccg cctcagaccg
      721 ccttccgacg gggacatcat cgtgcccaac gaggcggtct gcctcgcggg caccacctcg
      781 atcccggtgg acagccccga caacctggag atcgatccat ccgaggtcga caccgtgacc
      841 acccaggcgg agcagatggt gccggccttc ggcaccacga ggctcatccg cgcctacacc
      901 ggcgtgagac cgctgttgaa ggcgagcggg acagaggccg acggccggtc catctcccgc
      961 ggcttccaga tcatcgacca caagaacggc atgatcagca tcctcggcgg caagctgacc
     1021 acgtacaggc tcatggccga gaagacggtg gacgtcgtca tggatgtgct gggcttcaag
     1081 gtcccgtgca ggacagccga gctgccgctg gagggccagg acgagttgag cggctacccg
     1141 ctgtcgaaac gcctctcgaa acttgatgac atcgtctgcg agtgcgagct ggtgagccgg
     1201 tcggacgtcc agagggccgt caacgccatg gcgaccccct acgtgggcga catccagcac
     1261 cgaacgaggc tgggcatggg cccctgccag ggcgggttct gcacctaccg cgccctcggc
     1321 atcatgcagg agatgggccg ggtggaccca gacgtgtccc tgtcgatcct caaggagttt
     1381 ctccagagga ggttcaaggg catcaggccc gccctgtggg gggaccagct cagggaagag
     1441 cagctcatag agagcatata catgggcata ctcgccatgg ggcaaggcca tgggtgagca
     1501 cggctacgac gtggtcatta tcggcggcgg cgtggcgggc ctcacggcct ccatctacct
     1561 ggcaggaaaa gggttcagga ctgcgctcgt ctcgaggggg gacccggcct gctgcctctc
     1621 gaccggatgc atagacgtgc tcggggccca cgaggacccc atgaagggca ttgaccgcct
     1681 gccccgcaac catccctaca ggatcgcggg cagacgcacg gttatcgatg ccctcgaggc
     1741 gtttttaggg ctcatggaag aacggggcct cccctacagg ggctccctcg ggaggaacac
     1801 gggcattctc acccccatag gcacgaccaa gaccacctgc ctcgtcccca taaccatgga
     1861 ggcctcttcc ggcttcacaa cgacccacat ccacgtggtt tctttcaagg ggctcaggga
     1921 cttctacccg tcgtacataa cctcgaggct cgcgaacacg agcgtctcgg tgttcgacgc
     1981 ggggacctcg tcgaccctcg gcctcgcgac gaagttcgac gaaccctcgt tcgtggacga
     2041 tttctgcgcc tggctatccg gtctcgagct cccgccggg
//
LOCUS       DAOSCR010000500         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35470_length_2077_cov_3.917409, whole genome shotgun sequence.
ACCESSION   DAOSCR010000500 DAOSCR010000000
VERSION     DAOSCR010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35470_length_2077_cov_3.917409"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2068)
                     /locus_tag="PLT69_10860"
     CDS             complement(<1..2068)
                     /locus_tag="PLT69_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ14984.1"
                     /translation="MGSPFITVEVHAPASDDVGWLNAGATIIVHGDATNGVANAMAQG
                     RIYIAGDIGARGMTMTKHNPRFDPPELWVLGGTGDSFAEFMAGGIAVVCGIDSRSKEG
                     YILGHRPCVGMVGGKIFFRGHQKEISDADAVFVRITDEEWDWLRSGLSRFLKAIGKEY
                     LEEELTHDRSAWELVVARRPSEKRTRDRIPMGRFRKEVWDRELGPGGLIGDISKVDSS
                     RIGVIETGELRRFVPHWENESAMSPCQAACPTGIPVQKRWSLVRMGEIEKAVDLALAY
                     TPFPATVCGYLCPNLCMEACTRRAKGMTPVDTSVLGRASIEAKDPSPSPPSGRKVAII
                     GGGPAGLSVAWQLWMKGHDPVVFDRDARLGGKIRDVIPFSRIPKEVFDRELRRVEEKI
                     TRVRLEDPFSEQLFEDIVNRHDFTVIAVGANDPRTLSVKGARRTIAALDFLRAAKRNK
                     MKVKGNVVVIGAGNVGCDVATEAYRLGASSVTLVDIQKPASFGKEREAAEALGAKFVW
                     PVRTERITDDGVVLSDGSTIPADMVVVSIGDTPNLEFLPDTIDTESGMIRVDERYRTS
                     RDRVYAIGDCVRQGLITDAIGAGRIVASDIDARLKGEDATFDRMPSVDVARIRLEYYE
                     PQALTEHDDLASCAQTCASCGLCRDCGMCEAVCPTQAISRRELQGGLFENAADPGKCI
                     GCGFCAG"
BASE COUNT          319 a          743 c          637 g          378 t
ORIGIN      
        1 cgcctgcgca gaagccgcat cctatgcact ttcccgggtc agccgcgttc tcgaagaggc
       61 ctccctgaag ttccctcctc gagatcgcct gtgtgggaca gacggcctcg cacatgccgc
      121 agtcgcggca gagaccgcag gacgcgcacg tctgggcgca tgacgcgagg tcgtcatgct
      181 cggtgagggc ctgcggctcg tagtactcca gcctgatcct ggcgacgtcg accgagggca
      241 tgcggtcgaa ggtggcgtcc tcgcccttga gccgcgcatc gatgtcggat gccacgatcc
      301 tgccggcgcc gatggcgtcc gtgatgagcc cctgcctgac gcagtcgccg atggcataga
      361 cgcggtcgcg ggacgtcctg tagcgctcgt ccaccctgat catccccgac tcggtgtcga
      421 tcgtgtccgg gaggaactcc aggttcgggg tgtccccgat ggagaccacg accatgtcgg
      481 ccgggatcgt cgacccgtcg gacaggacca cgccgtcgtc cgtgatccgc tcggtgcgca
      541 cgggccacac gaacttcgcc ccgagcgcct cggccgcctc ccgttccttc ccgaaggagg
      601 ccggcttctg gatgtccacg agcgtcacgc tcgacgcgcc gagcctgtag gcctcggtcg
      661 ccacgtcgca cccgacgttg ccggctccta tcacgaccac gttccccttc accttcatct
      721 tgtttctctt ggcagccctc aggaagtcaa gagccgcgat ggttctccgt gcccccttga
      781 cgctcagcgt tcgcgggtcg ttggctccca cggcgatcac ggtgaagtcg tgcctgttca
      841 cgatgtcctc gaagagctgc tcggagaagg gatcctcgag cctcacgcgg gtgatcttct
      901 cctcaacccg ccgcaactcc cggtcgaaga cctccttcgg gatcctgctg aacggtatca
      961 cgtcgcggat cttgcccccc aggcgtgcat ccctgtcgaa caccacgggg tcgtggccct
     1021 tcatccacaa ctgccaggcc acggacaggc ctgcgggccc ccctccgatg atcgccacct
     1081 tcctccccga ggggggcgat ggggatggat ccttggcctc gatgctcgcc ctgcccagga
     1141 cggacgtgtc cacgggcgtc atgcccttgg cccggcgcgt gcacgcttcc atgcacaggt
     1201 tagggcacag gtaaccgcaa accgtggccg ggaagggggt gtatgcgagc gcgaggtcca
     1261 ccgccttttc gatctccccc atccttacca gcgaccagcg cttctgcacc gggatgcccg
     1321 tggggcacgc agcctggcac ggtgacatgg cgctctcgtt ttcccagtgc gggacgaacc
     1381 gcctgagctc cccggtctcg atcacaccga tgcgggagga atcgaccttc gagatgtcac
     1441 cgatgagacc cccgggaccc agctccctgt cccacacctc cttccggaaa cgccccatgg
     1501 ggatcctgtc cctggtccgc ttctccgagg gccggcgggc gacgacaagc tcccaggcgg
     1561 acctgtcgtg cgtgagctcc tcctcgaggt actccttgcc gatggccttc aggaacctgg
     1621 agaggccgga cctcagccag tcccactctt cgtcggtgat cctgacgaac accgcatccg
     1681 cgtcgctgat ctccttctgg tggccccgga agaagatctt gccgccgacc atgcccacgc
     1741 acggccggtg cccgagtatg tagccttcct tggagcggga gtcgatgccg cataccacgg
     1801 cgatccctcc ggccatgaac tccgcgaacg agtcgcctgt cccgcccaac acccagagct
     1861 cgggcgggtc gaagcgcggg ttgtgcttgg tcatggtcat cccgcgggcg cctatgtcgc
     1921 cggcgatgta aatcctgccc tgggccatgg cgttggcgac accgttggtg gcgtctccgt
     1981 ggacgatgat cgtcgcgcct gcgttgagcc accccacgtc gtcggaagcc ggagcgtgca
     2041 cctcgacggt gatgaaaggc gaacccatgg cgcccac
//
LOCUS       DAOSCR010000501         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35559_length_2074_cov_2.829123, whole genome shotgun sequence.
ACCESSION   DAOSCR010000501 DAOSCR010000000
VERSION     DAOSCR010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35559_length_2074_cov_2.829123"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLT69_10865"
     CDS             complement(<1..885)
                     /locus_tag="PLT69_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ14985.1"
                     /translation="MTPKIWASFTREEEWVVMNVKSIVCLVGALWISCCTLAWAREDA
                     KGCTDHPLFSRMEGFFIARCVQEDFASETFVDPETKKKVSVEGRKYTIDYGLKQGFEK
                     RYSRLQISRNYLNAITKIGGAGYENHPQWPDSTYMKITKDGKEIWAAFWQGYAGLAGR
                     LCIVEKQAMKQEITADFKSMAEGIHTTGHVALYGILFDFNKADVKPESEPALTEITKL
                     LKQDPRLKLFVVGHTDNVGGTDYNMKLSQQRAEAVVRELVTRYRIAPERLRAFGVGPL
                     APVASNDTEDGRAKNRRVE"
     gene            complement(1184..1648)
                     /locus_tag="PLT69_10870"
     CDS             complement(1184..1648)
                     /locus_tag="PLT69_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOJ14986.1"
                     /translation="MGRTVAGMIFWVGVSLGAGWFGSRFQPGRWYADLVKPSWTPPDA
                     VFAPVWTTLYVLMGVAAWTVWRARGFAGARLALSLFFCQLLLNALWSFVFFGLHRPGL
                     AFLEIVALWSAVLATTIAFWKKSAPSGLMLAPYLAWVSFAAVLNLWIWRLNT"
     gene            complement(1764..>2074)
                     /locus_tag="PLT69_10875"
     CDS             complement(1764..>2074)
                     /locus_tag="PLT69_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ14987.1"
                     /translation="GVRWEAIDDRSARGVLTDGAVTLSLLFTFGEDGLIETVCAQDRP
                     RMVDGRLVPTPWCGRFWNYQERSGMMVPVDGEVSWLLPGGARPYWRGRLTDIVYEFAR
                     "
BASE COUNT          382 a          715 c          601 g          376 t
ORIGIN      
        1 ttccacgcgg cggttcttgg ccctgccgtc ctccgtgtcg ttggacgcga cgggcgccag
       61 ggggcccacc ccgaaggctc tgaggcgctc cggcgcgatc ctgtatctcg tgaccagctc
      121 cctgaccacg gcctcggccc tctgctggga gagtttcatg ttgtagtccg tcccgccgac
      181 gttgtcggtg tggccgacca cgaagagctt gagcctcggg tcctgcttca ggagtttcgt
      241 gatctccgtg agggccggtt cggattccgg cttcacgtcc gccttgttga aatcgaagag
      301 gatcccgtag agggcgacgt ggccggttgt gtgaatcccc tcggccatgg acttgaagtc
      361 ggccgttatc tcctgtttca tggcctgctt ctcgacaatg cagagcctgc ccgcgagccc
      421 cgcatacccc tgccagaacg cggcccagat ctcctttccg tccttcgtga tcttcatgta
      481 cgtgctgtcc ggccattggg ggtggttctc gtacccagcc ccgccgatct tggtgatggc
      541 attcaggtaa ttcctggaga tctggagccg gctgtacctc ttctcgaacc cttgcttgag
      601 gccgtagtcg atggtgtatt tccggccctc gacgctcacc ttcttcttcg tttcaggatc
      661 gacgaacgtt tcagaggcga agtcctcctg cacgcacctc gcgatgaaga acccctccat
      721 tctcgaaaag agcgggtgat ccgtgcaccc cttcgcgtct tcccgggccc atgcgagcgt
      781 acagcatgag atccacaggg cccccaccag gcatacgatc gacttgacgt tcatcacgac
      841 ccattcctcc tctctcgtga atgacgccca tatcttcggc gtcatacaaa acaggctcac
      901 caccatgaaa cgaatgtcac gcctattttc tcttaaagcc tattgaataa tgacatgcca
      961 tcccggaagc aatgaccttc attccccttg cccgcaagga tcctcaagaa ccaccctcgc
     1021 ctgggacttt gcgcctatcg cgagaaggac ggtgcgcggg tcgcggagat cgcacttcct
     1081 ttgccgggcg acgggtccat gggttgactg cagccggggg ttcgggtgga aggccgtgaa
     1141 gggcggtccg tcatgccact atgcgccgtt tgccgatcgg cactcatgtg ttgagccgcc
     1201 agatccagag gttcaagacg gccgcgaagc tcacccacgc gaggtagggc gccagcatga
     1261 gaccagaggg cgccgacttc ttccagaatg cgatcgtggt cgcgagcacc gccgaccaca
     1321 gcgccacgat ctcgaggaac gcgagccccg gccggtgcag gccgaagaac acaaaggacc
     1381 acaaggcgtt caagaggagc tggcagaaaa acagcgagag ggccaggcgc gcccctgcga
     1441 acccgcgcgc ccgccagacg gtccacgccg cgacccccat cagcacgtac aatgtcgtcc
     1501 atacgggggc gaacacggca tcgggcgggg tccagctcgg cttgacaagg tcggcgtacc
     1561 accggcccgg ctggaaccgg gaaccgaacc accccgcacc cagtgaaaca ccgacccaga
     1621 agatcatccc tgcaaccgtg cgtcccatcc ctcgctcctt tccgcctggg gacacgcctc
     1681 ccgtcagcct tcggcacggc cagccgaagg aggcatcacc cgcaccaagg ccgattgggg
     1741 gtttgccccc caggcgcgcc cgctcaccgg gcgaactcgt agacgatgtc cgtgagacga
     1801 ccacgccaat agggccgcgc ccctccaggc aacagccacg agacctcgcc gtcgactggg
     1861 accatcatgc cggagcgctc ctggtaattc cagaagcggc cgcaccaggg tgtcggcacg
     1921 agccggccgt ccaccatccg agggcgatcc tgcgcgcaca ccgtctcgat gagcccgtct
     1981 tcaccgaacg tgaagagcag cgacagcgtg accgcgccgt cggtgaggac gccccgcgcg
     2041 ctcctgtcgt cgatcgcctc ccagcgcaca cctg
//
LOCUS       DAOSCR010000502         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35679_length_2069_cov_3.519364, whole genome shotgun sequence.
ACCESSION   DAOSCR010000502 DAOSCR010000000
VERSION     DAOSCR010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35679_length_2069_cov_3.519364"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLT69_10880"
     CDS             <1..533
                     /locus_tag="PLT69_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOJ14988.1"
                     /translation="LASVFHAITSSEPEALDAMNPETRGAFSGIVMKCLAKDPAERFQ
                     TGSALARELGGFLAGRTSAPGAAPRPRHRRTIAAAAVVAVACLVLGGILLYRATVSKA
                     PVETADLRVESVPEGAQVYVDGRFGGKTPARVKVSEGSHEVRLSMPGYYEWEAVVQAQ
                     RGSSTPLKVELVPSAE"
     gene            618..>2069
                     /locus_tag="PLT69_10885"
     CDS             618..>2069
                     /locus_tag="PLT69_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ14989.1"
                     /translation="MRHTMRVYVCIAAAAMFLAARPGDASAVQEGGKAPLFSLKGLDG
                     KVYSLASMEKSPMLVLYFFDASSRPSQEGLLSLDGIAKRYRKADLTVWGVTASAMPKA
                     QDFVNRTRPGFPVLLDTAGVGDAYGARQILPSVYILGPGLKVLDVIQGGGKSMAVMLT
                     RLAERKLQQKDTDLAMALGDAAAKTGPGGEKALAVKGYAALKAGRVDQAEKTFAELAR
                     GKGKAEVLGKEGLAAVYAHRGETDKAFELASQVERMAPERGYAHVVKADVLYARNKKS
                     EAMKEYEAATKKPDTDMFQKALAYNKLGRLHASVKDYKTSRELYEKAVTLDPYYVEAM
                     ANKGLAYEKEGRWDKALDAYREGQATGVRDDWTRILAARAMEMMEYQKDEKRRERVDR
                     LVKELSQRFREQKRAPAREDTWTSPPMVLGFVDFQEQGMLSERDGMSIVLTAQLAENL
                     KASGRVKVVDRVLLDRLLEELNLGSSELADGDTA"
BASE COUNT          390 a          631 c          757 g          291 t
ORIGIN      
        1 acctggcctc ggtcttccac gcgataacgt ccagcgaacc cgaggccctg gacgccatga
       61 accccgagac ccgaggcgca ttctcgggca tcgtgatgaa gtgcctggcc aaggaccctg
      121 cagaacgctt ccagacgggc tcggcgctgg cacgcgagct cggggggttc ctcgcgggcc
      181 ggacgagcgc gcccggcgcc gcgccccgtc cccggcacag gcggaccatt gcggccgcgg
      241 ctgtggtcgc cgtcgcctgc ctcgtcctgg gagggatcct cctgtatcgg gccaccgtca
      301 gcaaggcgcc cgtggagacg gccgacctcc gggtcgagag cgtcccggaa ggcgcacagg
      361 tttacgtgga cggcaggttc ggggggaaga cgccggcccg cgtgaaggtg tccgagggct
      421 cccacgaggt gcggctcagc atgccggggt actacgagtg ggaagcggtg gtccaggcgc
      481 agcggggatc atccaccccc ttgaaggtgg aactggtgcc ctccgctgag tgacggcgga
      541 gtgaggcggt gaagggatgg ggtttcttcg gtcgtttacg gccccgcgcc ggcatggcgg
      601 gggcaaggag ggatgagatg agacatacca tgagggtcta cgtctgcatc gccgcggcgg
      661 ccatgttcct cgcggcccgg ccgggggatg cctccgccgt ccaggaggga ggcaaggcac
      721 cgctgttctc gctgaaaggg ctcgacggaa aggtctacag cctcgcttcc atggagaaaa
      781 gcccgatgct ggtcctgtac ttcttcgacg cctcctcgag gccctcgcag gagggtctct
      841 tgagcctgga cgggatcgcg aagaggtaca ggaaggccga cctgaccgtg tggggcgtca
      901 ccgcctcggc catgcccaag gcccaggatt tcgtgaacag gacgaggccc ggcttccccg
      961 tgctcctcga caccgccggc gtcggcgatg cctacggagc ccgacagatc ctgccctctg
     1021 tctacatcct gggccccggc ctcaaggtgc tggacgtgat ccagggcggg ggcaagtcca
     1081 tggccgtgat gctcacgcgg ctcgcggaga ggaagctcca gcagaaagac accgatctcg
     1141 ccatggcctt gggagacgcg gcggccaaga ctgggccggg cggggagaag gccctggcgg
     1201 tgaaggggta tgcggcgctc aaggccgggc gggtcgacca ggcggagaag acctttgccg
     1261 aactcgccag gggcaagggc aaggccgagg tcctgggcaa ggaaggactc gccgcggtct
     1321 acgcgcacag gggagagacc gacaaggcgt tcgagctcgc gtcccaggtc gagcgcatgg
     1381 cgcccgagag ggggtatgcg cacgtggtga aggccgacgt cctctacgcc aggaacaaga
     1441 agagcgaggc gatgaaggag tacgaggccg cgacaaagaa acccgacacg gacatgttcc
     1501 agaaggccct cgcgtacaac aagctgggcc gtctccatgc gagcgtgaag gattacaaga
     1561 cctcccggga actctacgag aaggccgtga ccctcgaccc gtactacgtg gaagccatgg
     1621 ccaacaaagg tctcgcgtac gagaaggagg gcaggtggga caaggcgctg gacgcctacc
     1681 gtgagggaca ggcgacgggc gtccgggacg actggacgag gatcctcgcg gcccgggcca
     1741 tggagatgat ggaataccag aaggacgaga agcgcaggga gagggtggac aggctcgtga
     1801 aggagctctc ccagaggttc cgcgaacaga agagagcccc cgcccgggag gacacgtgga
     1861 cctcgccgcc catggtgctc ggtttcgtgg atttccagga acagggcatg ctctcggagc
     1921 gtgacggcat gtcgatcgtg ctgacggcgc agctcgccga gaacctcaag gcctcgggga
     1981 gggtcaaggt ggtggaccgc gtgctcctcg acaggctcct cgaggagctg aacctggggt
     2041 cctccgagct cgccgacgga gataccgct
//
LOCUS       DAOSCR010000503         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35736_length_2067_cov_2.503976, whole genome shotgun sequence.
ACCESSION   DAOSCR010000503 DAOSCR010000000
VERSION     DAOSCR010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35736_length_2067_cov_2.503976"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..760)
                     /locus_tag="PLT69_10890"
     CDS             complement(50..760)
                     /locus_tag="PLT69_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ14990.1"
                     /translation="MRRPSADLIVAITVIVMAAWAAPPAAHGQGVGDTLQEAGTPGQG
                     HPTGVARQGAEGGGEQKAGALTLERALDLALTNNAAIRESLSRRLASIEGRKAALADF
                     FPKLGAAYSYTNLEDQPYTIVGGTKLPVSSDEVFRWQVYAAQPIFTGFALSSRYEMAR
                     LGVDLGEAECDLARMETTRRVKEAYYGCLLAKRALDVSVEATDALMAHVKDAERFYDQ
                     GMIPFNDLLKSKVALAAA"
     gene            complement(757..1428)
                     /locus_tag="PLT69_10895"
     CDS             complement(757..1428)
                     /locus_tag="PLT69_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ14991.1"
                     /translation="MSEKQGNERSAGKGTLQRRARQKLYHRRQILDTALRLFSEHGFH
                     NVTMHRIAQESEFSIGTLYNFFENKEELYRALVLDLCREMESALSDALSGPGEEDQLI
                     RAYLEAKGRILMNNVPMLRIYFAQTRGASLEIMSALDQEVRAMYERFLRRLAKVFSSG
                     TRKGLFKKMLKPYYMAVALESITNAFLYLWLEDPDRHPYEKNIRDVARLFLENIKAGP
                     EGGRS"
BASE COUNT          343 a          677 c          668 g          379 t
ORIGIN      
        1 ggccggatat cgcggcctcg aggttcgccc gggcccgctc ttcggactgc tacgccgcgg
       61 cgagggcgac cttcgacttg agcaggtcgt tgaaggggat catgccctgg tcgtagaagc
      121 gctcggcgtc cttgacatgg gccatgaggg cgtcggttgc ctcaacggac acgtcgaggg
      181 ccctcttcgc gaggagacag ccgtagtagg cctccttcac ccgcctggtc gtctccatcc
      241 tggcgaggtc gcactcggcc tcgccgagat ccacgccgag gcgcgccatc tcgtagcggg
      301 aagacagggc gaaaccggta aagatgggct gggccgcgta aacctgccag cggaacacct
      361 cgtcgctgct caccggcaac ttcgtccccc ccacgatcgt gtacggctgg tcctcgaggt
      421 tcgtgtacga gtatgcggcg ccgagcttgg ggaagaagtc ggcaagggcc gccttcctcc
      481 cctcgatgga ggcgaggcgg cggctcaagg actccctgat cgcggcgttg ttcgtcaggg
      541 cgagatccag ggccctctcg agcgtaaggg cgcctgcctt ttgttcgccc cccccttcgg
      601 cgccttgccg ggcgacgcct gtggggtggc cctggccggg cgtgcccgct tcctgcaggg
      661 tatcgcccac cccctgccca tgggcggcag gcggggcggc ccacgccgcc atgacgatga
      721 cggtgatcgc tacaataagg tctgccgaag gtctcctcat gagcgccctc cctcgggccc
      781 cgccttgatg ttctccagga agagccgcgc cacgtcgcgg atgttcttct cgtaggggtg
      841 ccggtcaggg tcctcgagcc acaggtacaa gaaggcgttc gttatgctct cgagcgcgac
      901 cgccatgtag tacggcttga gcatcttctt gaagagaccc ttcctggtgc cgctcgagaa
      961 caccttggcg agcctcctga ggaacctctc gtacatggcc cggacctctt ggtcgagcgc
     1021 gctcatgatc tccagggagg cgcccctggt ctgcgcgaag tagatcctca acatcggcac
     1081 gttgttcatg aggatgcggc cctttgcctc gaggtaggcc ctgatgagct ggtcctcctc
     1141 ccccggcccg gagagcgcat ccgacagggc gctctccatc tcccggcaca ggtcgagcac
     1201 gagggcccgg tagagctcct ccttgttctc gaagaagttg tagagggtgc ctatggagaa
     1261 ctcggactcc tgtgcgatgc ggtgcatggt cacgttgtgg aacccgtgtt ccgagaagag
     1321 cctcaaggcg gtgtccaaga tctgcctgcg atggtagagc ttctggcgcg cccgtctctg
     1381 gagggtgcct ttgcctgcag aacgctcgtt cccctgtttt tcgctcatgc cgaaaccccg
     1441 ctgcacctcc accccgcctc gtgcgaccct gccggacgga cgcggcgcgg ggcaggaatg
     1501 cggtgcggat ccatcccagg cattgcccgt gccatggtcg cccatcctgc agccgctttc
     1561 ggcacaggcc atgaggcggc ccccaggggg catcccctca tgccgggcgg ggatcgtcga
     1621 ggcgttgagg cagcgacggt tgaacggtgc tccccggggc ggctcgcaca cggcccgggc
     1681 gatcgcccgg acgccagctc gaacgcgcca ggggagggaa cccccttcgt ccgtatcccc
     1741 ggaaacgcca aaccaaagac ctgcctcttg ggccctgaag acacgaaacc ccctcagtgt
     1801 atccgcacgc gaccactggc cagtaataat gaatatccaa tcaaaaaatg aactaggtgt
     1861 caaggaatga aaattcattc ttgatttacg gggatgcgta cctgcgccgt ggtcatgaag
     1921 gcaagagggc gccgggcatc tcctgaagac gcgtggggcc gcaggcgccg tgggcatctc
     1981 ttttggcccg gcgacctgtc gatcccctcg cacctgaaga cgcttggggc cgcaggcgtc
     2041 atgggcatga atgcgggagg cgaagat
//
LOCUS       DAOSCR010000504         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_35819_length_2064_cov_2.782977, whole genome shotgun sequence.
ACCESSION   DAOSCR010000504 DAOSCR010000000
VERSION     DAOSCR010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35819_length_2064_cov_2.782977"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLT69_10900"
     CDS             complement(<1..341)
                     /locus_tag="PLT69_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOJ14992.1"
                     /translation="MIAAFAWIASQPVSPIEAHEKITERPATPSRVVSLAPSVTETLY
                     ALGLGEAVAGVTSFCVFPPQARAKPTVGGYVNPSYEAVFALNPDLVIGLPEHADILAR
                     LSRAGLRTLGV"
     gene            complement(571..864)
                     /locus_tag="PLT69_10905"
     CDS             complement(571..864)
                     /locus_tag="PLT69_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOJ14993.1"
                     /translation="MKEFAKPSYPRSSRVAGEEGTAVFDVEISSDALPVRITLVKSSG
                     HGALDEAALEALRKSSYVPAMENGIPVPSVRRVEVRFSLGEAVNVAGAGGKGK"
     gene            complement(1601..>2064)
                     /locus_tag="PLT69_10910"
     CDS             complement(1601..>2064)
                     /locus_tag="PLT69_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ14994.1"
                     /translation="FDSVNWKYLNISGAKTRGVEATLSARPSGGLDIDASYTFTDTED
                     DATGKELLRRPRSKASIAASSRLGESLGIRVQASYTGPRYDVYYDPATWAQQRVRLGG
                     YATAGMRAWYDLDARMRVTLEVDNLFDRKYEEVYGYQTPGRCIGVGLRASM"
BASE COUNT          304 a          748 c          649 g          363 t
ORIGIN      
        1 ctgacgccga gcgtgcggag ccccgcacgg gagagtctcg cgaggatgtc cgcatgctcg
       61 ggaagcccga tgacgaggtc cgggttcaac gcaaagacgg cctcgtagct cgggttcacg
      121 tacccgccaa cggtgggctt cgccctggcc tggggcggga agacgcagaa actcgtcacc
      181 ccggccacgg cctcgcccag gcccagcgca tagagggttt ccgtcacgct cggagccagc
      241 gagacgacac gcgaaggggt tgcgggccgc tcggtgatct tctcgtgcgc ctcgatggga
      301 gagacaggct gggacgcgat ccacgcgaag gccgcgatca cgatccacca ccgtgcccct
      361 gcgggggacg ggcctcttct cgtcgtgtcg cgcaccgagg gcatcaccgc tcgatccgcc
      421 cctttcctcg agcccttcgg agaaggggtg gaaggacaag gccgtcgcgg acgagaacac
      481 ctcagccttg cacctgcgtg tcgccccacc gtgccacccg ctcggtctgc aggaaacgac
      541 ctcgctcttg ctccgaacct gacgaacacg ctatttcccc tttcccccgg cgccggcgac
      601 gttcaccgct tctcccaggg agaacctcac ctcgaccctc ctcaccgaag ggaccggtat
      661 gccgttttcc atggcaggca catagctgga tttcctgagc gcctcgaggg cggcctcgtc
      721 gagtgcgccg tggccgctgg atttcaccag ggtgatgcgc accggcaggg cgtcgctcga
      781 gatttccacg tcgaagaccg ccgtgccctc ctcgccggcc accctggacg acctcgggta
      841 gctgggcttc gcgaactcct tcacccctgc cctctgcgag gccgcttgcc catgcccccc
      901 cacacgatcg ccaggggtgc ccgcgaggcc cgcctccgcg tcacccgggc ctccctgcgt
      961 gcccgcaccc gtgccttcac cttgcgcctg gggggtctcc gcgaccagtg cgatcccgtg
     1021 cggttgcgct ccggcaggcg atgcatgttc agggaccggc gcctgtcttt gcgtggggcg
     1081 tgtgtcgacg gcccgtttgc cgtgaggaca gggacccgtc tcgtccgaac ggcgcacttc
     1141 ggcttcaggg gccttgcccg gccggggctt gcccggggcg gccccagaga ggcctttggg
     1201 gccgccttcg aactgcccga tctccacgat gctcacgatg catgcctctt ccttcaagaa
     1261 ggcatcgagg tgccgtcctt ccgcacggcc gaacacgagc aggcatccta tacccaggat
     1321 gtggacaacg gccgagacga gcaggcaacc acccgtgagt cggctcgtcg ccttgagtcc
     1381 gcgctcgcac cttcctgcgt caagccccgg gaacacccct gcgcccgtga tccccgtttc
     1441 acccctcacg ccacggcctc ccttgccgaa gcgatccacc gagggggccg cagggtcctt
     1501 gccctcaggc cctccggtga tgtacggcct tgccgatcgc cactcttctg acaaagacgg
     1561 cgccctgtac gttcagcggc tccacgtccc acacgcaccg tcacatgctc gccctcagcc
     1621 ctacgccgat gcacctgccc ggcgtttggt agccgtagac ctcttcgtat ttcctgtcga
     1681 acaggttgtc cacctcgagg gtgacgcgca tgcgggcgtc gaggtcgtac cacgccctca
     1741 tgcccgcggt cgcgtagccg ccgaggcgca cccgttgttg ggcccacgtg gcggggtcgt
     1801 agtacacgtc gtagcgcgga cccgtgtagg aggcctgcac ccggatgccg agggactccc
     1861 cgagcctcga ggatgcggcg atggatgcct tgctccgcgg tctcctgagg agctccttgc
     1921 cggtggcgtc gtcctcggtg tctgtgaagg tgtacgaggc atcgatgtcg agaccgcctg
     1981 aaggccttgc cgagagcgtg gcctcgaccc cgcgggtctt tgcgccgctg atgttgaggt
     2041 acttccagtt gacgctgtcg aaat
//
LOCUS       DAOSCR010000505         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36030_length_2055_cov_3.180000, whole genome shotgun sequence.
ACCESSION   DAOSCR010000505 DAOSCR010000000
VERSION     DAOSCR010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36030_length_2055_cov_3.180000"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PLT69_10915"
     CDS             complement(<1..1607)
                     /locus_tag="PLT69_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ14995.1"
                     /translation="MRDIEEAIQGNEATFRVLVENANDVLWVFDLDLGYTYISPSVMR
                     LRGYSVEEAVKLRLDQVLTPESYARVREMLGRELDLMKAGYRHGPGWFTTLELEMVRK
                     DGSTFWAEVIVNLLYDEEGSLKGIMGITRDITKRRAAEEELKRCRDDLEEQVVKRTSE
                     LVLINERLMREIEERSRIEAELRESAEMYRVHLSLTNDVMFSYDNQFRVLSVSPNVTR
                     VLGYTPEELVGKTFQEAGVLDPSFMPKAFENALHILAGNPIYSSVYLFITKDGRRRYG
                     EVSGVPCMRDGKVYAVTTVARDITERLEMENALRQSEERYKATLECMPAAVCIVRVSD
                     SRCMYANQAFSAITGYTVEEALGRTTSELDLPPVDELVSPKEGPGECEGRDLEHTLKR
                     KDGGVIKARLSTRPVQYDSQACLVMAITDMTSLMQLEEERARLEAQRLKMDAIATLAR
                     GIAHDFNNILTTIIGYTNLSLRNVKGLEEKGSGIETILRDLDEVRRAAKRAVTLVNHI
                     QAFSRHPEREHVPVDLCTLVDRTIESLG"
BASE COUNT          304 a          693 c          617 g          441 t
ORIGIN      
        1 cccaggctct cgatcgtcct gtccacgagc gtgcagaggt cgacggggac gtgctccctc
       61 tcggggtgcc tgctgaaggc ctggatatgg ttcacgagcg tgacagcccg tttcgccgcc
      121 cgtctcacct cgtccaagtc tctcaggatc gtctctatgc ccgagccctt ctcttccagg
      181 cccttgacgt tcctgagaga caggttcgtg tacccgatga tggtggtgag gatgttgttg
      241 aaatcgtggg ctatgccccg ggcgagggtc gcaatggcgt ccatcttcag tctttgcgct
      301 tccagcctcg ccctctcttc ctcgagctgc atgagcgagg tcatgtcggt gatggccatg
      361 accaggcagg cctgggagtc gtattgcaca ggtctcgtgg agagacgcgc cttgatgacc
      421 ccgccgtcct tcctcttcag ggtgtgctcg agatccctgc cttcgcactc gcccggtccc
      481 tccttcggcg agacgagctc gtccacgggg gggaggtcga gttcactcgt cgtcctgccc
      541 aaggcctcct cgacggtata ccccgtgatc gcggagaatg cctggttcgc gtacatgcac
      601 ctcgagtccg acaccctcac gatgcacacc gccgcgggca tgcactccag cgtggccttg
      661 tacctctcct cgctctgcct gagggcgttc tccatctcca ggcgctcggt tatgtccctc
      721 gcgaccgtgg tcacggcgta gaccttgccg tcacgcatgc agggcacgcc gctcacctcc
      781 ccgtacctcc tcctcccgtc cttggtgatg aacaggtaga ccgaagagta gatggggttt
      841 cccgcgagga tatggagcgc gttctcgaag gccttgggca tgaacgaggg gtcgagcacg
      901 cccgcctcct ggaaggtttt cccgacgagt tcctcgggcg tgtacccgag gacgcgcgtg
      961 acgttcgggg agacgctcag caccctgaac tggttgtcat aggagaacat cacgtcgttc
     1021 gtgagcgata agtgcacgcg gtacatctcc gcgctttccc tgagctcggc ctcgatcctc
     1081 gatcgttcct cgatctcccg catgagccgt tcgttgatga ggacgagctc ggacgtgcgc
     1141 ttgacgacct gctcctcgag gtcgtccctg cacctcttga gctcctcctc cgcggccctt
     1201 cgtttcgtga tgtcccgggt gatgcccatg atgcctttca gggagccttc ctcgtcgtag
     1261 aggaggttca cgatcacctc ggcccagaag gtcgagccgt ccttgcggac catctcgagc
     1321 tcgagcgtcg tgaaccagcc ggggccgtgg cggtaccccg ccttcatgag atcgagttcc
     1381 cggccgagca tctcgcggac cctcgcgtac gactcgggcg tcagcacctg gtcgaggcgg
     1441 agcttgacgg cctcctccac gctgtagccc ctcaggcgca tgacggacgg gctgatgtag
     1501 gtgtagccga gatcgaggtc gaacacccag aggacgtcgt tcgcgttctc cacgaggacc
     1561 ctgaacgtgg cctcgttccc ctggatcgcc tcttcgatgt ctctcatggt ccgctctcct
     1621 cccggacagg tgcgcggcca cgcccgccct cttgaccgcg atgcgcacgc gccgatgttg
     1681 ccgccccctg tcggactccc cggtcatccc cagggaaccg tccgaccggt ctcttggaca
     1741 atggccttta taaacccctg gtacccgatt gtgtcaaccc gcacgcctgt gggtatgacc
     1801 ggcgccgggt tcgcccccgt tcgttcacta cccgtctatc ccgcgcgcct gtgggtatgg
     1861 tcctttcgcc gagggcgggg ccttccttgt cgcatccggg tgcgatgccg gcaatgcaca
     1921 ccctcggggc tgaacgttgt tcatgggtct attagtaagg gtaagccact cgaatggcaa
     1981 gggaacgcgc atgggacggc cgggcgcatc gggtctttcc gcatgagacc ctcgccccgg
     2041 actcggccga tcgtg
//
LOCUS       DAOSCR010000506         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36045_length_2054_cov_3.602301, whole genome shotgun sequence.
ACCESSION   DAOSCR010000506 DAOSCR010000000
VERSION     DAOSCR010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36045_length_2054_cov_3.602301"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLT69_10920"
     CDS             <1..471
                     /locus_tag="PLT69_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOJ14996.1"
                     /translation="VGEVSCTGLHGANRLASTSLLEGLTWGYYAAVDITERINSGKTV
                     SPKVFSQVRDWIPTGGVMEKEDPALIAQDWMVIKNTMWNYVGIVRTSARLKRAIEDMR
                     HMYQRIEEFYKETPISKDIISLFHGTQSAIMVAEAALRNRTSIGCHYINGPMNP"
     gene            complement(568..1977)
                     /locus_tag="PLT69_10925"
     CDS             complement(568..1977)
                     /locus_tag="PLT69_10925"
                     /inference="COORDINATES: protein motif:HMM:NF039222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ14997.1"
                     /translation="MDCFFNPRGIAVIGASDNQHKGGYHLFRNIIQSYRGPVYPVNPR
                     LGEIMGRECYPDVRSIPHDFDLAVFFVPAGNLPRVIEDCAAKGVRGIIIESAGFAEVG
                     QEGRNLQETSVALARRHGIRLWGPNCMGLLDAHRGHVFSFMYTDAWKRLMRPGDVSMI
                     VQSGMLSAGFLMMILERGLLDISKMCSIGNKCDIHETELLAHLVDDPTTAVIGMYVES
                     IVEPRRFMELVAGTNKPVVVLKGGRSPKGARAAMSHTASLASNHAIVRAALRQANAVE
                     VTDVNELMDVLRGFSKSGRCRFSDGTGAAVITFSGGGGIVTIDLLEEAGVPLAELGSQ
                     TLAALGEVYPPWMSPSNPADIWPAIEHSGPRKVYETALKAVMEDPAVDSLVMHLFTSM
                     IDGSMFAELARLKEELGKPVVAWLAGVGDTFRTLRSELEDLGIPTFDEMQRCVSVLAA
                     IRRHARDSTHKQPRTGRQG"
BASE COUNT          372 a          662 c          633 g          387 t
ORIGIN      
        1 gtcggggagg tctcgtgcac cgggctccac ggcgcaaaca ggctcgcctc cacgtcgctc
       61 ctcgaggggc tcacctgggg ctactacgcc gccgtggaca tcaccgagag gatcaacagc
      121 ggcaagaccg tgagcccgaa ggtcttctcc caggtgaggg actggattcc gaccgggggg
      181 gtgatggaga aggaagaccc ggccctcatc gcgcaggact ggatggtcat caagaacacc
      241 atgtggaact acgtgggcat cgtgaggacc tctgcccgcc tcaagcgcgc catcgaggac
      301 atgcgccaca tgtaccagag gatagaggag ttctacaagg agaccccgat atccaaggac
      361 atcatcagcc tgttccacgg gacccagtcg gcgatcatgg tggccgaggc cgcgctgagg
      421 aaccgaacct cgatcgggtg tcactacatc aacggcccca tgaaccccta gcggcggccg
      481 tccaccgtga cgaaggtgga ttgacgagtc atcctccttc acgccctcga tgatgaaacg
      541 gtgggtgcgg aggcctgccg gagcgcctca cccctgtcgg cctgtcctgg gctgcttgtg
      601 ggtgctgtcc ctcgcatgcc tgcgaatcgc cgccaggacc gagacgcacc tttgcatctc
      661 gtcgaaggtc gggataccca ggtcctcgag ctccgatctc agggtcctga acgtgtcccc
      721 gacgcccgca agccaggcga cgaccggttt tcctagctcc tccttgaggc gcgcaagctc
      781 cgcgaacatg gagccgtcga tcatggaggt gaagaggtgc atgacgaggc tgtcaacggc
      841 cgggtcctcc atgacggcct tcagggccgt ctcgtagacc ttcctcgggc cgctgtgctc
      901 tatggcgggc cagatgtccg ccgggttcga ggggctcatc cacggcgggt acacctcgcc
      961 gagggccgcg agcgtctggc tgcccagttc ggcgagcggg acgccggcct cttcgaggag
     1021 gtctatggtg actatcccgc ctccccccga gaaggtgatg accgccgccc cggtgccatc
     1081 cgagaacctg caccgccccg acttggaaaa acccctgagc acgtccatga gctcgttgac
     1141 gtccgtgacc tcgaccgcat tcgcctgcct gagggcggcc ctcacgatcg cgtggttgga
     1201 ggcgaggctc gcggtgtgac tcatggccgc cctggcaccc ttggggcttc tgcccccctt
     1261 gagaacgacc acaggcttgt tcgttcccgc aacgagttcc atgaatctcc tcggttccac
     1321 gatggattcc acgtacatcc ctatgacggc cgtggtcggg tcgtcgacga gatgggccag
     1381 gagttcggtc tcgtggatgt cgcacttgtt gcctatcgag cacatcttgc ttatgtcgag
     1441 caggccccgt tccagtatca tcatgaggaa ccccgcagag agcatgccgc tctggacgat
     1501 catggaaacg tcgccgggtc tcatgagcct cttccacgca tccgtataca tgaaggagaa
     1561 gacatggccc ctgtgcgcgt cgagcagccc catgcagttg gggccccaca gccgtatgcc
     1621 gtgcctcctg gcgagggcca cggacgtctc ctgaaggttt ctgccctctt ggcccacctc
     1681 ggcgaaccct gcggactcga tgatgatgcc ccgcaccccc tttgcggcgc agtcctcgat
     1741 gacccttggc aggttccccg ccgggacgaa gaatacggcc agatcgaaat catgggggat
     1801 cgatctcacg tcagggtagc actcgcgccc catgatctcg cccaggcgcg ggttcaccgg
     1861 gtagacgggc cccctgtaac tctggatgat gttcctgaag aggtggtaac cacccttgtg
     1921 ctgattgtcg cttgctccta tgacggcgat tcccctgggg ttgaagaaac agtccattgg
     1981 cgggagtcct ctcctggctg cgaaatgtgt cgtgcacgaa ctgcataatc gagaagggat
     2041 gcctgtgtca aatg
//
LOCUS       DAOSCR010000507         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36125_length_2051_cov_2.610220, whole genome shotgun sequence.
ACCESSION   DAOSCR010000507 DAOSCR010000000
VERSION     DAOSCR010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36125_length_2051_cov_2.610220"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            802..1188
                     /locus_tag="PLT69_10930"
     CDS             802..1188
                     /locus_tag="PLT69_10930"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ14998.1"
                     /translation="MKLSEYFEQARGKGVLATADSSGQVNAALYAKPHFIDESTVAFI
                     LAPRLTHGNLETNPYAAYIFIEDGEGFKGRRLYLTKIKEEKNSELIATLRRKKRSVSH
                     DEAIDEDRFLVFFRIDRVLPLVGSGA"
     gene            complement(1408..>2051)
                     /locus_tag="PLT69_10935"
     CDS             complement(1408..>2051)
                     /locus_tag="PLT69_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ14999.1"
                     /translation="PMTGAVLMALASALVISWVRVALREREDTVIQAVWAVGMAIGLL
                     FIFKTPGYNEDLMSYLFGSILMVTQRDLWVMAGLDVMLLVVCVPLYSRMVAVCFDEEF
                     AVTRGIDVQLNHLLMICLVALTVVLLVSVAGIVMVIALLTLPAATASHFSGRLWHMMV
                     LSGVLTAVLSIAGMAVSYEPDLPVGAVIIVLAACVYILVVITARARGIRGMRA"
BASE COUNT          395 a          691 c          613 g          352 t
ORIGIN      
        1 gacaagcaga catgcccgca cccagcctca ggggtggact tcttgggata ttgcatcctt
       61 tcatctcctc gtgcatcaat acatataccg acaaagattt taacctcctc ttgaggatgg
      121 cgcgtcctat gccctaaccg ccatcctgtt tttttgccct cgtctcggca cggaagaggg
      181 acccgtcgtt cgcgccggag cgggtgagga cagcgcatcg ttccatgcac ccctgggtgg
      241 tcgtcgaccc cgggcgcggc gtacagcgtc acgggccggc gttccatgca caccttgatg
      301 tccgcgcgag gatggttggg gccgttcttg gggttaccgg cgttccatgc acacctggat
      361 gatcggcatg accgcagcgt atacacgcga aaccattgca gcgtttcatg cacacctgga
      421 tgaccgcctc gtggagatcc tgctccccgg ccctggaaac cgcgttccgt gcacatctag
      481 ttgatcgggc ccccgtcccg cccggcccgc gccctcgccc gggacaccgg ccccggggtg
      541 cgccgccctc ggggggccgg ctgccctctc aggatggaaa cttccagacc gtgccaggaa
      601 aacacttcct cacgcccacg ccctctgcca cccggttctc accctccgtt acgccctggt
      661 acgggaatgg gggctttttc cgttttttaa aaggccccat ggcacccggc ttgagggttg
      721 ggcctcccgg cgagccccac aaaaacccgc ttgattttct cccgtgcgcc cctatgagaa
      781 agggcagaaa ggagatgcac gatgaaactc tccgagtact tcgagcaggc acggggcaag
      841 ggtgtgttgg ccacagcgga ttcctccggg caggtaaacg ccgcactgta cgccaaaccc
      901 cacttcatcg acgagtctac cgtggccttc atcctggcgc ccaggctcac ccacggcaac
      961 ctggaaacca acccttacgc cgcgtacatc ttcatcgagg atggggaggg gttcaagggc
     1021 aggagactct acctcaccaa gatcaaggag gagaaaaaca gcgaactcat cgccaccctc
     1081 aggcggaaaa agcgttccgt ctcgcacgac gaggcgattg acgaggacag gttcctggtc
     1141 ttcttccgca tcgacagggt cctccccctg gtgggctccg gggcatagcc tgcaggcccc
     1201 acccggcgga gcgccgggcc ctgtcttccc ctatccacat tcagctggat cttaggcccc
     1261 acccgggctg agtgccggga cgccgggtgc acacccgggc aggtccaggg tttccagccc
     1321 ccaccgctgg tgaccgcact cggggtgcgc catgggttcg ggcaagaccg ggcggcgaga
     1381 actccccttt ggtcaggccg ccgtgtttca ggccctcata ccccgtatgc ccctggccct
     1441 ggccgtgatc accacgagga tgtacacgca ggcagcaagc acgatgatga cggcgcccac
     1501 cgggagatcc ggctcgtagc ttacggccat gcctgcaatc gagagtacgg cggtcaggac
     1561 accagagagc accatcatgt gccagagcct gcccgagaag tgtgaggcgg tggccgcggg
     1621 cagggtgagc agggcgatca ccatgacgat gcccgccacg ctcacgagaa gcaccacagt
     1681 gagggcgacg aggcagatca tgagcaggtg gttgagctgc acgtcgatgc ccctggtgac
     1741 cgcgaactcc tcgtcgaagc acacagccac catgcggctg tagagcggga cgcacacgac
     1801 gagcagcatg acgtcgaggc ccgccatgac ccacaggtct ctctgggtca ccatgaggat
     1861 gctcccgaac aggtagctca tgaggtcctc gttgtaaccg ggggtcttga agataaagag
     1921 cagcccgatg gccatcccga ccgcccacac ggcctgtatg accgtgtcct cccgctcacg
     1981 cagggcgacc ctcacccacg agatgacgag cgccgacgcc aaggccatga gaaccgcgcc
     2041 tgtcatgggg g
//
LOCUS       DAOSCR010000508         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36238_length_2046_cov_3.321949, whole genome shotgun sequence.
ACCESSION   DAOSCR010000508 DAOSCR010000000
VERSION     DAOSCR010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36238_length_2046_cov_3.321949"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /gene="thiC"
                     /locus_tag="PLT69_10940"
     CDS             complement(<1..1084)
                     /gene="thiC"
                     /locus_tag="PLT69_10940"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOJ15000.1"
                     /translation="MTTRQCAASGITTAEMVRVAAYEGLDPEYIRRGVAQGTIVIPKN
                     ARRTFELLRGIGKGLRVKINANIGASPKDSSVERELEKLRAAVHHGADAVMDLSLGED
                     IEAVRAQILAASPVMVGTVPVYETVYRLARAGRRLEEMTIDDFLETVRSQAEQGVDFM
                     TIHAGVNLQALSALESSGRRLGVVSRGGSMLVRWMRHNRKESPLFEHFDEVLDILAAY
                     DVTISLGDGLRPGAVLDATDRPQVTELVTLGALTERAWDKGVQVIVEGPGHVPLDRIE
                     ENVRLEKDLCRGAPFYVLGPLVTDCAAGYDHIAGAIGGALAAFFGADFLCYVTPGEHL
                     RLPGVAHVVEGVIASRIAAQAADLARG"
     gene            complement(1081..>2046)
                     /gene="thiE"
                     /locus_tag="PLT69_10945"
     CDS             complement(1081..>2046)
                     /gene="thiE"
                     /locus_tag="PLT69_10945"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOJ15001.1"
                     /translation="GLRVAEDVCRYAWGNQGLASLLREARHKAFSIVDRGECMKARNV
                     STDVLAGADASRARSGEDLSDLVQANLKRAEEAFRVLAEVLRPHRPGAARAFEELRYQ
                     VYHLEQTALARRASRLPGGLYLILNAPSERFEKLAGEAVSAKVGVIQLRCKKTGDRVF
                     LETACRLRSLIGDKGPLLIVNDRVDIALMSGAHGVHLGQDDAEASRARDLMGPGAIIG
                     LSTHTIPQVRTANTLPVDYIGFGPVFEPFSKQDHERPTGIEALEEAVRVSCVPVIAIG
                     GIGLEHARRLRATGCHGIASIGAVERAADPGGMIRAMNTAFLEGS"
BASE COUNT          315 a          717 c          659 g          355 t
ORIGIN      
        1 gcccccttgc gaggtccgcc gcctgtgcgg ctatgcgcga ggcgatcacg ccctccacca
       61 cgtgggccac gccggggagc ctcagatgct cccccggcgt cacgtagcag aggaagtccg
      121 ccccgaagaa cgcggcgagc gccccgccga tggcgcccgc tatgtggtcg taccctgccg
      181 cgcagtccgt gaccaggggg ccgagcacgt agaagggggc gccccggcag aggtctttct
      241 cgagccgcac gttctcctcg atcctgtcca gcggcacgtg gcccgggccc tcgacgatca
      301 cctgcacccc cttgtcccag gccctctcgg tgagggcgcc cagggtgacg agttcggtga
      361 cctgcggccg gtcggtcgcg tccagcacgg cgccgggccg cagcccgtcg cccagcgaga
      421 tcgtcacgtc gtatgcagcg aggatgtcga ggacctcgtc gaagtgctcg aacagcgggg
      481 attccttccg gttgtgtctc atccacctca cgagcatcga gccgcccctg ctcaccacgc
      541 ccagtcgacg gcccgaagac tccagggcag agagcgcctg gaggttgacc ccggcgtgaa
      601 tggtcatgaa gtcgaccccc tgctcggcct gggaccggac ggtctcgagg aagtcgtcga
      661 tggtcatctc ctcaagacgc ctcccggccc tggcgagcct gtacacggtc tcgtagacgg
      721 ggaccgtgcc gaccatgacc ggcgaggccg cgaggatttg ggccctgacg gcctcgatgt
      781 cttcgccgag ggagaggtcc atcaccgcgt ccgcgccgtg gtgcacggcg gcccgaagct
      841 tttcgagctc ccgttcgacg ctggagtctt tgggcgaagc ccctatgttc gcgttgatct
      901 tcacgcgcag ccctttcccg atgccccgga ggagttcgaa ggtgcgcctt gcgttcttgg
      961 ggatcacgat cgtgccctgg gcgacgcccc tccgaatgta ttccgggtcg agcccctcgt
     1021 acgcagccac cctcaccatc tcggcggtgg tgatacccga tgccgcacat tgcctggtcg
     1081 tcatgagccc tccagaaacg ccgtgttcat ggccctgatc atgccccccg ggtcagccgc
     1141 acgctccacc gccccgatgg aggcgatgcc gtgacacccg gtggcccgaa gacgcctggc
     1201 atgttccagg ccgataccgc cgatggcgat tacgggcaca cacgagacgc ggacagcctc
     1261 ctcgagggcc tcgataccgg tcggcctttc gtggtcctgc ttggagaaag gttcgaagac
     1321 gggtccgaac ccgatgtagt cgaccggcag ggtgttggcg gtcctgacct gcggtatcgt
     1381 atgggtggaa agcccgatga tcgccccggg ccccatgagg tcgcgtgcgc gtgacgcctc
     1441 agcgtcgtcc tgtcccaggt ggacaccgtg ggcgccgctc atgagcgcga tgtccacccg
     1501 gtcgttgacg atgaggagcg ggcctttgtc cccgatcagg gatctcaagc gacaggcggt
     1561 ctccaagaag acccggtcac cggtcttctt gcacctgagc tggatcacgc cgaccttggc
     1621 cgagaccgcc tcgccggcga gtttctcgaa gcgctcggat ggagcgttca ggatgaggta
     1681 gagacccccg ggcagcctcg acgcccggcg tgcgagcgcg gtttgctcca ggtggtagac
     1741 ctggtagcgg agctcctcga atgctcgagc ggcccccggc cggtgcggac gcaagacctc
     1801 cgcgagaacc ctgaacgcct cctcggcgcg cttcaggttc gcctgcacga gatccgagag
     1861 gtcttccccc gacctggccc ggcttgcatc ggcccctgcg agcacgtccg tggagacgtt
     1921 ccttgccttc atgcattcgc cccgatccac gatcgagaag gccttgtgtc tcgcttcccg
     1981 gagcaaggat gcaagcccct ggttgcccca tgcatacctg cacacgtctt ctgcgaccct
     2041 gaggcc
//
LOCUS       DAOSCR010000509         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36283_length_2044_cov_3.890397, whole genome shotgun sequence.
ACCESSION   DAOSCR010000509 DAOSCR010000000
VERSION     DAOSCR010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36283_length_2044_cov_3.890397"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="PLT69_10950"
     CDS             <1..1689
                     /locus_tag="PLT69_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ15002.1"
                     /translation="LEDELFADYLVSKDGRMTALYVVFDEIDSAHVDEIIAQVRAVMD
                     RGKPASLEVFLSGGLMVSSEFVKATRQNQTLLPALGIAIGILTIYVLFRSFSRIVIVT
                     VVVGLSLVWTLGLHSLMGYAFNPISGMIIPLVVVLSLSNSIHMMEYYDEVRKAHAPGA
                     SFLSMVRFITVPCFIASITTSLGLFSLMTSPIRAVKQFGFTSGAGIFFSFLISLFVVP
                     FLLTVTPDRRVREHRWWGRSLSLVARANERRHGLILVITILLTVASAVGMGRVTIDSN
                     ELEWFPEDSELRKNAMKFDRALSGIGNVELVLVGEPGMLMDPKVLSGMDRLSRRIERM
                     PNVRKAISLADYIKAVNRTLKGGDETHYRVPDTTELIAQEALLFSMSQTGREEIERYA
                     NIDNSVGRISVRLKYTSSAEVRELMSRIRSEIANVFPDGGVRVGLTGFSFIFSMIDKY
                     IVESQIRSFSLAFVLVFGVMFLVMWSVRYGALAIIPNVLPVMFIIGFMGWTGINLNVG
                     TVMIASVALGIAVDDTTHLISRFRREYDTRAQPCTGRSGSRSSGWAGPWSSLPS"
     gene            1668..1904
                     /locus_tag="PLT69_10955"
     CDS             1668..1904
                     /locus_tag="PLT69_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15003.1"
                     /translation="MVFTSLIAVCGFCVVVLSEFQPARDFGVLLSFTLVIALICDMFL
                     LPSMIIVLRRFLLGSGHASGDGGPSPAPGPSDPR"
BASE COUNT          354 a          632 c          635 g          423 t
ORIGIN      
        1 ctcgaggacg agctgttcgc cgactacctc gtctcgaagg acggccggat gacggccctc
       61 tacgtagtgt tcgacgagat cgacagcgcg cacgtcgacg agatcatagc gcaggtcagg
      121 gcggtcatgg atcgcggaaa gccggcctcg ctggaggtct tcttgagcgg cggcctcatg
      181 gtgagcagcg agttcgtcaa ggccacccgc cagaaccaga cgctccttcc cgccttgggc
      241 atcgcgatag gtatcctgac catctacgtg ctcttcaggt cgttctccag gatcgtgatc
      301 gtcaccgtcg tggtggggct gagcctcgtg tggacgttgg ggctccactc gctcatgggg
      361 tacgccttca accccataag cggcatgatc atcccgctcg tcgtggtgct ctcgctgtcg
      421 aacagcatcc acatgatgga gtactacgac gaggtcagga aggcgcacgc gcctggggcc
      481 tccttcctgt ccatggtgcg cttcatcacc gtgccctgct tcatcgccag catcaccacg
      541 tcccttgggc tcttctcgct catgaccagc cccataaggg cggtcaagca gttcggtttc
      601 acctcgggag ccgggatttt cttttcgttc ctcatctcgc tcttcgtggt tcccttcctc
      661 ctgaccgtga ccccggaccg tagggtccgg gaacaccgct ggtgggggcg ctcgctgtcc
      721 ttggtcgcgc gcgccaacga aaggcgtcac gggctcatcc tcgtgatcac gatcctcctg
      781 accgtcgcct ccgccgtggg catgggaagg gtcacgatcg actcgaacga actcgagtgg
      841 tttcccgagg acagcgagct caggaagaac gcgatgaagt tcgaccgggc gctctcgggc
      901 atcgggaacg tggaactcgt gctcgtgggc gaacccggca tgctcatgga cccaaaggtg
      961 ctttccggca tggacaggct ctcccgcagg atcgagcgga tgccgaacgt gaggaaggcg
     1021 atctccctgg ctgactacat caaggcggtc aaccggaccc tgaagggagg ggacgaaacg
     1081 cactacaggg ttcccgacac caccgagctg atagcccagg aagccctcct cttcagcatg
     1141 tcccagaccg gcagggaaga gatcgaacgc tatgccaaca tcgacaactc cgtgggcagg
     1201 atatcggtga ggctgaagta cacgagctcg gccgaggttc gagagctcat gtccaggatc
     1261 cgctcagaga tcgcgaacgt attccctgac ggcggtgtca gggtcgggct gaccgggttc
     1321 tcgttcatct tcagcatgat cgacaagtac atcgtggaga gccagatacg atcgttctcg
     1381 ctcgccttcg tcctcgtgtt cggggtcatg ttcctcgtca tgtggtcggt gaggtacggg
     1441 gcgctggcga tcatcccgaa tgtgctgccc gtcatgttca tcatcgggtt catggggtgg
     1501 accggcatca acctgaacgt ggggaccgtg atgatcgcat cggtggcatt agggatcgcg
     1561 gtggacgaca ccacgcacct gatcagcagg ttccgcagag agtacgacac ccgggcgcaa
     1621 ccctgcacag ggcgctcagg cagtcgatca tcagggtggg cagggccatg gtcttcactt
     1681 ccctcatagc agtatgtggt ttctgcgtgg tcgttctctc cgagttccag cccgcgaggg
     1741 atttcggggt gctcctgtcg ttcacgctcg tgatagcgct catatgcgac atgttcctcc
     1801 tcccgtccat gatcattgtc ttgagaaggt tcctgctcgg ctccgggcac gcatctggcg
     1861 atggcggccc ttcgcctgcg cccgggccga gcgaccctcg gtgacggcct tcgcccgcct
     1921 ccttcgggta gatacccttc gagggaccat cgaggatgcc tcggtggcgc tgcgtacggg
     1981 gacgtgccgg catcctgacg gaaaggcagc cttcggggtt cggcggcacg gagctctcct
     2041 ttct
//
LOCUS       DAOSCR010000510         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36353_length_2041_cov_3.341390, whole genome shotgun sequence.
ACCESSION   DAOSCR010000510 DAOSCR010000000
VERSION     DAOSCR010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36353_length_2041_cov_3.341390"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLT69_10960"
     CDS             <1..744
                     /locus_tag="PLT69_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HOJ15004.1"
                     /translation="KGATDRAIRDGMDILEAAFASGSSKAEALRKAVSAFKSIPVSRQ
                     VRPKVAIVGDLYVRDNDFLNQDLITFLEERGAEAVTMPYTTYARMVAGPYLRKWVHEG
                     KYLFALSSAAMLAAFRAMEMRYYGIFREVLDEPEPTFTDNPARILAAFGLTTRHSGES
                     MDNVLKVHYLSKHYSDIGLLVLVNPAFCCAGLVTEAMADRIEQVTGIPVANIVYDGTG
                     GTKNSSLVPVLAHLRSSASRAADLLGLAG"
     gene            complement(909..1268)
                     /gene="atpE"
                     /locus_tag="PLT69_10965"
     CDS             complement(909..1268)
                     /gene="atpE"
                     /locus_tag="PLT69_10965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOJ15005.1"
                     /translation="MKRIGRVSGVSVFFVMVFASAVFAAEAAASPEAIQYFSWVAVAT
                     GIGMAIAAFGTGIGQGNGVRGACEGIARNPDASGKILTALILGLAMIESLAIYALVIE
                     LILLYANPFLKFMGLGA"
     gene            complement(1547..>2041)
                     /gene="atpB"
                     /locus_tag="PLT69_10970"
     CDS             complement(1547..>2041)
                     /gene="atpB"
                     /locus_tag="PLT69_10970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOJ15006.1"
                     /translation="GLKSLLVDTMGEHGMRFFPLIATIALFIFTCNMAGIIPGFYSPT
                     ANLNTNLSMALTVFFLTHIVGVRIHGVKYLKHFMGPVWWMAPIMIPVELIGHLARPLS
                     LTMRLFGNIFGEDLVLVVLLALVPFLVPMPMLALMIFTSVLQAFVFTLLAMMYISGAM
                     EEAH"
BASE COUNT          391 a          621 c          675 g          354 t
ORIGIN      
        1 aagggcgcaa cggacagggc gatcagagac ggtatggata tcctggaagc ggccttcgcc
       61 tccggctcgt ccaaggccga ggcgctcagg aaggccgtct cggccttcaa gtccatcccc
      121 gtctcgaggc aggtgcggcc caaggtggcg atcgtgggag acctctacgt cagggacaac
      181 gatttcttga accaggatct cataacgttt ctcgaagaga ggggggccga ggccgtcacc
      241 atgccgtaca ccacctatgc gcggatggtc gcaggcccct acctgaggaa gtgggtacac
      301 gagggcaaat acctcttcgc cctctcctcc gccgccatgc tcgcggcctt ccgtgccatg
      361 gagatgcgct actacgggat cttccgcgag gtcctggatg agcctgagcc caccttcacg
      421 gataacccgg cccggatcct cgccgccttc gggctgacca ccaggcactc cggcgagtcc
      481 atggacaacg tgctgaaggt gcactacctc tcgaagcact acagtgatat cggcctgttg
      541 gtgctcgtca acccggcgtt ctgttgcgcc ggtctcgtga cggaggccat ggcggaccgc
      601 atcgagcagg taacgggcat acccgtggcc aacatcgtct acgacgggac cggcggaacg
      661 aagaactcgt ccctcgtgcc ggtgctggcg cacttgaggt cttccgcctc acgcgccgct
      721 gaccttctgg gactcgccgg gtagggtgga ctgggtctgg caggctgacg gcccctgatg
      781 tccatgaggg catggaacct cgcggagagg gctgctggtg ggtggtcacc gggagatcgc
      841 ccggggagag gagtggaaac gggccccatg gtatggggcc cgtaggctta cgagcgacgg
      901 tgaccttcct atgcgccgag ccccatgaac ttgaggaacg ggttcgcgta gagaaggatg
      961 agctcgatga ccaatgcgta gatggcgagg gactcgatca tcgcgaggcc cagaatgagc
     1021 gccgtgagga tcttccccga ggcgtcgggg ttgcgggcga tgccttcgca ggcccccctc
     1081 acgccgttgc cctggccgat gcccgttccg aatgcggcga tcgccatgcc gatacccgtg
     1141 gcaacggcaa cccaggagaa gtactggatg gcctcggggc tcgctgctgc ctcggctgcg
     1201 aataccgctg acgcgaaaac catgacgaag aatactgaaa cccctgaaac ccgaccgatt
     1261 ctcttcatac attcctccat caataagtat tctcagatga ttgccgcgat tctcaatcga
     1321 gcttactcta cccgcgatag gcggtcgagg tcaatagcct tgtagggcgt gcagtggatc
     1381 tccgccgtga tcgaacacgc gcaccgcggc tgcccggggc gctcgggccg ggcatggatg
     1441 acgccccgga cggggcggtc ttcctggaca gggggcctcg accttgcctc tcggccaggc
     1501 cgaggccccc tcggtgtcgt cgtgtgatgg gcgccggcgc cggtaatcag tgcgcctcct
     1561 ccatcgcgcc gctgatgtac atcatggcga gcagggtgaa cacgaaggcc tggagcaccg
     1621 acgtgaagat catgagggcg agcatgggca tggggacgag gaagggcacc agggcgagga
     1681 gcaccaccag gaccaggtcc tcgccgaaga tgttcccgaa gagccgcatg gtgagcgaga
     1741 gcggccttgc cagatgcccg atgagctcca ccgggatcat gatgggggcc atccaccata
     1801 ccgggcccat gaagtgcttg aggtacttga cgccgtgaat cctcacgccc acgatgtgcg
     1861 tcaggaagaa gaccgtcagg gccatggaca ggttcgtgtt gaggttcgcc gtcggcgagt
     1921 agaaccccgg gatgatgccc gccatgttgc aggtgaagat gaagagggcg atggtcgcga
     1981 tcagcgggaa gaacctcatg ccgtgttccc ccatggtgtc cacgaggagc gacttgaggc
     2041 c
//
LOCUS       DAOSCR010000511         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36374_length_2040_cov_3.129975, whole genome shotgun sequence.
ACCESSION   DAOSCR010000511 DAOSCR010000000
VERSION     DAOSCR010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36374_length_2040_cov_3.129975"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLT69_10975"
     CDS             <1..894
                     /locus_tag="PLT69_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOJ15007.1"
                     /translation="STHGKPLLISSFFGLEDHCVRTFHERRIPTFDAPEKAARAMGAF
                     LRHLEIRNRPVDRQGRIGSPPEEATAIVKRAGGAGLDEYDAKALLRSYGIPTPVEVRV
                     STLAAAREAARSIGFPVALKACAPEIRHKTELGLVRLGIASEDELERAYRSMSERAGV
                     PGFIVAEMLRGERELMAGVTRHPGFPPCVVLGLGGVFAEALEDISSRFAPLSATDAHG
                     MMDGLRSSRLLGPFRGMKAVDRVALSNILVALGRLALHFPHIAEVDLNPIIVVDGSPI
                     VADALVVLGEGDTEKGIENAR"
     gene            1030..1425
                     /locus_tag="PLT69_10980"
     CDS             1030..1425
                     /locus_tag="PLT69_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15008.1"
                     /translation="MSDARIRDMIARNPGGGKLQCAVAHYIAASLGVAPLEVGREATD
                     MGVKLDQCQLGLFGYGRKGRSSYKILGRKVEVEKEVLDAIRSAARGGRVACAELWRIA
                     DAMGLARAEVGNAADSLGIKIAPCQLGAF"
     gene            1905..>2040
                     /locus_tag="PLT69_10985"
     CDS             1905..>2040
                     /locus_tag="PLT69_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ15009.1"
                     /translation="MALIVTWLGHASVRITGPSGTVYIDPWKTPKGPKADIILLTHDH
                     Y"
BASE COUNT          343 a          633 c          748 g          316 t
ORIGIN      
        1 tccacccacg gcaagcccct gctcatatcc tcgttcttcg gcctcgagga ccactgcgtc
       61 cgcaccttcc acgagagacg cataccgacc ttcgacgcgc ccgagaaggc tgcacgcgcc
      121 atgggcgcgt tcctcaggca cctggagatc aggaaccggc ccgtggacag gcagggccgc
      181 atcgggagcc cccccgaaga ggcgacggcc atcgtgaagc gggcaggcgg cgccggcctc
      241 gacgagtacg acgcaaaggc gttgctccgg tcctacggga tacccacgcc cgtcgaggtg
      301 cgcgtctcga ctcttgcggc cgccagggag gccgcacgtt cgatcgggtt ccccgtggcg
      361 ctcaaggcgt gcgcacccga gatcaggcac aagaccgaac tcgggctcgt gaggctcggg
      421 atcgccagcg aggacgaact ggaaagggcg tatcgctcca tgagcgagag ggcgggcgtg
      481 ccaggcttca tcgtcgccga gatgctccgc ggcgaacggg agctcatggc cggcgtcacc
      541 aggcaccctg gttttccccc gtgcgtggtc ctggggctcg gcggcgtgtt cgcggaggcg
      601 ctcgaggaca tctcgtcgag gttcgccccg ttgagtgcca cggacgccca cgggatgatg
      661 gacggcctca ggtcgtccag gcttctcggg ccgttcaggg gcatgaaggc ggtggacagg
      721 gtggcgctgt caaacatcct cgtcgccctg ggaaggctcg cgctgcactt tccccacata
      781 gcggaggtcg acctgaaccc catcatcgtg gtggacggct ccccgatcgt ggcggacgcc
      841 ctcgtggtgc tcggtgaagg ggacacagaa aagggcatcg aaaacgcccg atgatggaca
      901 tggtcgttgc ccgggatagg cggtcgcggt gaagggggtg cggatggtga gggaaggacc
      961 gttctcggcg tgggccaacg gggagtgcgg tggcctatgg gccgggcggc cctgtggaag
     1021 gagagaggca tgagcgatgc acggataagg gacatgatcg cgaggaaccc cggcgggggc
     1081 aagctgcagt gcgctgtcgc gcattacata gcggcctctc tcggggtagc ccccttggaa
     1141 gtcggcaggg aggcgacgga catgggcgtc aagctcgacc agtgccagct cggcctgttc
     1201 gggtacggcc gcaaggggag atcctcgtac aagatcctcg gcaggaaggt ggaggtcgaa
     1261 aaggaggtcc tcgacgcgat ccgctcggca gcacgaggcg gcagggtcgc ctgcgccgag
     1321 ttgtggagga tcgccgacgc catgggcctg gccagggcgg aggtcggcaa cgccgcggac
     1381 tcgctcggca tcaagatagc accgtgccag ctgggcgcgt tctgaggagg atccggtccg
     1441 cggtgcccct ccgggccggc ccgatgagcg tgccctgctt aggtgtcgtg cacctgggcg
     1501 ggcaaggccg cgggttgctc ccgcctgtgt atcgaagcag gccgtgaggg cggcgggggg
     1561 ccgagggcgg cgcgccctgc gggcctgcag acgggcgggc ggtgtacgcg atacccaagg
     1621 ccgggcgaaa accctggcgc acgaccccgg ggcgtcgttc cgtcccggcc cgggcgaacg
     1681 cggcgcgccc ccggcatggg agtggagagg tttttgcgcc ggtgccgcgc ttctctgcag
     1741 tcaacgcggg ggtgaaagct ccacccggca tgggagtggg gaggtttttg cgccggcgcc
     1801 gggcacgaac ctcggtgttt cccgttgaaa cgactgcggt gcttttgtat tattttacaa
     1861 tgaccccatc acgggcctga gaccatttcc cacaggaggt gcccatggcg ttgatcgtca
     1921 cctggctcgg ccatgcgagc gtcaggatca caggcccatc ggggacggtg tacatagatc
     1981 cgtggaagac gccaaagggc ccgaaggcgg acatcatcct cctcacccat gaccactacg
//
LOCUS       DAOSCR010000512         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36409_length_2039_cov_2.623992, whole genome shotgun sequence.
ACCESSION   DAOSCR010000512 DAOSCR010000000
VERSION     DAOSCR010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36409_length_2039_cov_2.623992"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PLT69_10990"
     CDS             complement(<1..1260)
                     /locus_tag="PLT69_10990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOJ15010.1"
                     /translation="MGIAILDSDAQTVNLIAEIVAETGETPHPFTSKDRFLELVDPHK
                     HSIAVVSEEFKDVIEGTKQVYDSIEIVGIGTSDGNACLQAGAAHYLRKPFKAEDLMEV
                     LGALRAVGGVAATNGEPSLVASDPSMLAVLETVRKVAPTQAPVLLMGESGTGKEVVAR
                     TIHALSRRKDGPYVGINLAAIPESLLESELFGYEKGAFTGAYATRIGKFEQAHCGTIL
                     LDEVTEIGFGLQAKLLRVLQERQIDRVGGKAPITVDFRVISTTNRDIEEEIRAGRFRQ
                     DLFYRLNVISIRIPALRDRPGDILPLARHFVAKVAHREGMREKRLSDGAKDALMRYPW
                     PGNVREMENVIERAMILADGDTITEDFIPSPNGAARAPGIEPAPGLTIQEMERRLITA
                     TLEKVGGNRTRPRPSSASASGPCATSST"
     gene            complement(1265..>2039)
                     /locus_tag="PLT69_10995"
     CDS             complement(1265..>2039)
                     /locus_tag="PLT69_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ15011.1"
                     /translation="VKDLADGPTLFVMGESLYRAGRLDEARDILERSTRTESLYKEQA
                     FSLLFFIDYKRERYQEALLWNTLCLGTFPKGKEATLMRRWRGFALVKTGNMDAAIPFL
                     EEATSPDDPFALSALDTLAQVYGAKGDFQRQQGALDRIVAFHPARVSPVIERALYSRA
                     EAKMRSGDLQGAKVLYETLLEAYPRSPRAHWAMYSLARVQLAEGDTEGAKERLETVKR
                     VSRDPVLVQAARTAIDEVDLENDLRLLKASGSLVRKTGE"
BASE COUNT          290 a          658 c          688 g          403 t
ORIGIN      
        1 cgttgagctt gttgcgcagg gtcctgatgc tgatgccgag gagggtcgcg gccttgtcct
       61 gttcccgccg accttctcga gggtggcggt gatgagccgg cgctccatct cctggatggt
      121 cagccccggg gccggctcga tgccaggcgc ccttgccgcc ccgttcgggg aagggatgaa
      181 atcctcggtt atggtgtccc cgtcggccag tatcatcgcg cgctctatga cgttttccat
      241 ctcgcgcacg ttcccgggcc agggatagcg catcagggca tcttttgccc cgtccgagag
      301 ccttttttcc ctcatgccct cgcggtgtgc gaccttcgcg acgaagtgcc tggcaagcgg
      361 caggatgtct cccgggcggt ccctgagggc cgggatgcgg atggagatca cgttcaggcg
      421 gtagaagagg tcttgccgga acctgcccgc cctgatctcc tcctcgatgt cgcgattggt
      481 ggtggagatg acgcggaagt ccaccgtgat cggggccttg cctcccaccc tgtcgatctg
      541 gcgttcctgg agcaccctca ggagcttcgc ctggaggccg aacccgatct ccgtgacctc
      601 gtcgaggagg atcgtgccgc agtgcgcctg ttcgaatttg ccgatcctcg tggcgtacgc
      661 gccggtgaac gcccccttct cgtagccgaa cagctcgctc tccagtagcg attccgggat
      721 ggcggcgagg ttgatgccga cataggggcc gtccttgcgc ctgctcaggg cgtggatggt
      781 gcgggcgacg acctccttgc ccgtgcccga ctcgcccatg aggagcacgg gggcctgggt
      841 cggggcgacc ttcctgacgg tctcgagtac ggcgagcatc gagggatctg aagcgaccag
      901 cgaaggctcc ccgtttgtcg ccgcgacgcc cccgacggcc ctcagggcac cgaggacctc
      961 catcaggtcc tcggccttga acggcttcct caggtagtgc gccgcgcccg cctggaggca
     1021 ggcgttcccg tcggaggtcc ctatgcccac gatctctatc gagtcgtaga cctgcttggt
     1081 cccttcgatg acgtccttga actcctcgga gaccaccgcg atcgagtgct tgtgcgggtc
     1141 cacgagctcg aggaaccggt ccttgctggt gaaggggtgg ggggtctcgc cggtctcagc
     1201 cacgatctcg gcgatgaggt tcacggtctg ggcgtcggag tcgagaatgg ctatccccat
     1261 ggtcctactc acccgtcttt ctgacgaggc tgcccgatgc cttgagcagc ctcaggtcgt
     1321 tctccaggtc gacctcgtcg atggccgtgc gggccgcctg gacgagcacc gggtctctgc
     1381 tgacacgctt gaccgtctcg aggcgttcct tggcaccctc cgtgtcgcct tcggcgagct
     1441 gcacccgagc gagggagtac atggcccagt gtgccctggg cgagcggggg taggcctcga
     1501 gcaacgtctc gtagaggacc ttcgcccctt gcaggtctcc cgagcgcatc ttcgcttcgg
     1561 cccttgagta cagggcgcgc tcgatgacgg gggacaccct ggccgggtgg aatgccacga
     1621 tcctgtcgag ggctccctgt tgcctctgga aatccccctt cgccccgtag acctgggcga
     1681 gggtgtcgag ggcgctcagc gcgaacgggt cgtcgggaga ggtcgcctcc tcgaggaacg
     1741 ggatcgcggc gtccatgttg cccgtcttga caagagcgaa cccgcgccag cgccgcatga
     1801 gggtcgcttc cttccccttg gggaaggtcc cgaggcagag cgtgttccac aggagggcct
     1861 cctggtagcg ctcgcgcttg taatcgatga agaagaggag cgagaacgcc tgctccttgt
     1921 acagggactc ggtcctggtg gacctttcga ggatgtcgcg tgcctcgtcc aggcggccgg
     1981 cccggtagag ggactcgccc atgacgaaca gggtggggcc gtccgcgagg tccttgacg
//
LOCUS       DAOSCR010000513         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36512_length_2035_cov_3.805051, whole genome shotgun sequence.
ACCESSION   DAOSCR010000513 DAOSCR010000000
VERSION     DAOSCR010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36512_length_2035_cov_3.805051"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLT69_11000"
     CDS             <1..256
                     /locus_tag="PLT69_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ15012.1"
                     /translation="EILESNLNDIISPFLKKLSSHYLNLTPTEIQVADLIKDGKSTKQ
                     IAEVLGISERAVEFHRNNIRDKLGLKNSKTNLRSYLMTLG"
     gene            complement(1067..>2035)
                     /locus_tag="PLT69_11005"
     CDS             complement(1067..>2035)
                     /locus_tag="PLT69_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15013.1"
                     /translation="VAFVPFYVPYRLPDREERWFPRVLVPPESVDAGATVGRVPVRTY
                     YRDIDLPPRTMRNAEAGIRVSRLVSGWDLSASYFTGFDPMPLTESVVDVDVTLVNPLS
                     LEYTAASRVTLMPALHRIHVFGFDFTGTLGDFTLRGEYAYFHGKRYNRRLESVLDELV
                     TRETIDDMYSDFLVDYFASGGRTTSKSFRLDAEAVSKMDSMKYGFGVDYIHGDTSLSV
                     QLIQEFVPDHDNARPVYFVKDGFDTLSTFLLKQFFLQNTLELNLRVAYDLEFHDHLVK
                     PSARYHFTDRLQGTVGAIVIGGGRKDSLLGQFGMNDQVYAQLRCSF"
BASE COUNT          390 a          637 c          643 g          365 t
ORIGIN      
        1 ggagatcctc gaatcgaacc tgaacgacat catctcccct ttcctgaaaa agctctcttc
       61 acactacctc aacctgacgc ccacggagat ccaggtggcg gacctcatca aggacgggaa
      121 gtccaccaag cagatcgcag aggtgctcgg catctccgag cgggccgtgg agttccaccg
      181 gaacaacatc cgcgacaagc tggggctcaa gaactcaaag accaacctcc gctcgtacct
      241 catgaccctc ggctaggcgc ttgcgccctc acttcaagag cgcaccaaag agaggcgcgc
      301 tcgaccttgt cgctgcgctt ggctggatgc cttaatccca ggcgcgaagg tccgccgggt
      361 tcgtcaaagt ggcgccagac ctcgttgcgg cgattgggct ggttgcggag atccccgacg
      421 caaaggtaag ccgggttcat ttcaaggagg cacaagacgc cctcctgccc ggccgggcag
      481 gagggctgaa gagagtgacc gtcctgtcac ctgctcccct gtatctcccg ataccagtgt
      541 gcccctgcgt tggtaatgaa gcttcattat ttttataaca aagatctcgt ccccatgcaa
      601 gacttttgtg cgcccctgtt ctgcatggtg ttcttcctca cggggcggtc cttctcaagc
      661 gacacacacg atgggcatcc tcgaagacgc gcctgaggca tgtccccgcc ccatgcagga
      721 gccgaagcag gcgttccggg cgcgcctctc ccggcgcggc cgatccttca cgcaggcata
      781 cctgggtatc cccgggacac cgagctgagc agacccggga gaggaagaac gataacccgg
      841 gcagggtgat caggccgggg tatccccggc acaccgggcg gcgcggtccc gggtggcgca
      901 ccctgcccgc ctcggcatgc ctgcattcac gccgggcagg catcacaggg agacgttctg
      961 aaacgtaccg ggcaggctgt cctcgccgat gcgggggctg tctgacaaca agcatcacag
     1021 ggggccgcct gttaacgtgc cggtgaaagg cttaggaccg ggcggctcag aaagagcacc
     1081 gaagctgcgc gtagacctgg tcgttcatcc cgaactgccc gaggagcgag tctttcctgc
     1141 cgccgccgat cactatcgcg cccacggtcc cctgcagcct gtcggtgaag tgatacctcg
     1201 ccgagggctt gaccaggtgg tcgtggaact cgagatcgta ggcgaccctg aggttgagct
     1261 ccagggtgtt ttggaggaag aactgcttga ggagaaaggt cgagagggtg tcgaagccgt
     1321 ccttcacgaa atagacgggg cgcgcgttgt cgtggtccgg cacgaactcc tgtatcagct
     1381 gtacgctcag gctcgtgtcg ccgtggatgt agtcgacgcc gaagccgtat ttcatggagt
     1441 ccatcttgga gacggcctcg gcatcgagcc tgaagctctt cgaggtcgtc ctcccgccgg
     1501 acgcgaagta atccacgagg aagtccgagt acatgtcgtc gatcgtctcc ctcgtgacga
     1561 gctcgtcgag cacgctttcg agcctgcggt tgtaccgctt gccgtggaag tatgcgtatt
     1621 cgccccggag cgtgaagtct cccagcgtgc ccgtgaagtc gaacccgaag acgtggatcc
     1681 ggtggagggc cggcatgagg gtgaccctcg aggccgcggt gtactcgagc gagagggggt
     1741 tgacgagggt cacgtccacg tccaccacgg attcggtcag gggcatggga tcgaacccgg
     1801 tgaagtagct ggccgagagg tcccagccgc tgacgagcct ggagaccctg atacctgcct
     1861 cggcgttccg catggtcctt ggcgggaggt ctatgtcacg gtaatacgtc cgcacgggga
     1921 ccctgcccac ggttgccccg gcgtccacgc tctcgggggg cacgagcacc ctggggaacc
     1981 accgttcctc gcggtccggc aaccggtacg gcacatagaa gggcacgaag gccac
//
LOCUS       DAOSCR010000514         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36678_length_2030_cov_3.031899, whole genome shotgun sequence.
ACCESSION   DAOSCR010000514 DAOSCR010000000
VERSION     DAOSCR010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36678_length_2030_cov_3.031899"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLT69_11010"
     CDS             <1..209
                     /locus_tag="PLT69_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOJ15014.1"
                     /translation="GMDVVIDSVNIDAENVTISGSTGTYDDVEKIKELLSGLPYLREV
                     KIVQANVDKNDQKVRMRLTCKRQV"
     gene            191..793
                     /gene="gspM"
                     /locus_tag="PLT69_11015"
     CDS             191..793
                     /gene="gspM"
                     /locus_tag="PLT69_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOJ15015.1"
                     /translation="MQETGLRKTLQDLKGRVTAMLRPAEALAGRAVDLLARLEPRERA
                     LVILAAVLLSILILAGGVYGPLLHYRFTLEKSISAKEEQLKKIYVQSETIRSLGRVVE
                     EERKGQQGSFTLFGFLEGLAVKLSINDRIEYMKPIADNQAAGRETVEVKIRGLSMEDL
                     IGLLLGVESSARPLSVKHLSIKRQEKDGGIDVTFQVVFYG"
     gene            845..1840
                     /gene="gspN"
                     /locus_tag="PLT69_11020"
     CDS             845..1840
                     /gene="gspN"
                     /locus_tag="PLT69_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HOJ15016.1"
                     /translation="MCLAVFTWVRFPYDALGTRIENGLGAFLGVPVRLGHIRPTWTGG
                     FSVQGVELQGNLIVKTLRLTPYPWEALRGVVGLGYRAEMPAGSLEGRVALPTRMSSRP
                     MDISLELNEVDLSGISAVFPPSWRPAGLVTGEVRMASPKGALDKMMGSMSISWEKGRL
                     PLGMPSLPFDALVFETMELEGTVEKGIVKIDRGEFTGEFSGNLTGMVRLLKDMRRSRL
                     NLTGEVSLPEAVRASLGAGSPPQGQPARFSLRGTMERPRFRIVGLTQPGGAPARPAVA
                     GASQGQVSAAALRRESERSRIRAGAEVTAPPDAGVGPGEQALDQADGEGFDMEGE"
     gene            1842..>2030
                     /locus_tag="PLT69_11025"
     CDS             1842..>2030
                     /locus_tag="PLT69_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15017.1"
                     /translation="MASMHEAGRFPKALRLSWNEVEGLLWDMAGSIRRDGYDPEVIIA
                     VARGGLVPARILADYLQKK"
BASE COUNT          382 a          606 c          725 g          317 t
ORIGIN      
        1 ccggcatgga cgtcgtgatc gactccgtga acatcgacgc cgagaacgtc acgatctccg
       61 ggagcacggg cacgtacgac gatgtcgaga agatcaagga gctcctgtcc ggcctgccct
      121 acctgagaga ggtcaagatc gtacaggcga acgtggacaa gaacgaccag aaggtgagga
      181 tgagactcac gtgcaagaga caggtctgag aaagacactg caggacctga aaggcagggt
      241 gacggccatg ctccggccgg cagaggccct cgcgggcagg gccgtcgacc tgttggcccg
      301 gctcgagccc cgggaacggg cgctcgtgat cctggcggcc gtgttgctgt cgatcctgat
      361 cctcgcggga ggggtgtacg gaccgctgct ccactaccgc tttacgctcg agaagtccat
      421 ctcggcgaag gaggagcagc tcaagaagat atacgtccag tcggaaacca tccgcagcct
      481 cggccgggtg gtcgaagaag aacgcaaggg ccagcagggc tccttcaccc tgttcggttt
      541 cctggaagga ctggcggtca agctgtccat caacgacagg atcgagtaca tgaagcccat
      601 cgccgataac caggccgccg ggcgcgagac ggtggaggtc aagatcaggg ggctctccat
      661 ggaagacctc atcgggctgc tcctcggcgt ggagtcctcg gccaggcccc tgtccgtcaa
      721 gcacctgagc atcaagaggc aggaaaagga cggcggcata gacgtcacgt tccaggtggt
      781 gttctatggt taggtcaagg gcgggaaaga acatctcggt gatccttctc ggctctacgg
      841 cctcgtgtgc ctggcggtct tcacctgggt gcgtttcccg tacgacgccc tcgggaccag
      901 gatcgaaaac ggccttggcg ccttcctggg cgtcccggtg aggctcgggc acatacgccc
      961 gacctggacg ggggggttct ccgtccaggg ggtggaactc cagggcaacc tcatcgtgaa
     1021 gaccctgagg ctgaccccat acccctggga ggcgctgcgt ggggtcgtcg gcctgggcta
     1081 ccgcgcggag atgcccgccg ggtccctcga gggaagggtt gcgctcccga ccaggatgag
     1141 ctcgcgcccc atggacatca gcctcgagtt gaacgaggtg gacctctcgg gcatctcggc
     1201 cgtgttcccc ccctcgtggc gcccggccgg cctcgtgacc ggagaggtga ggatggcctc
     1261 ccccaagggg gcgctcgaca agatgatggg gagcatgagc atctcctggg aaaagggaag
     1321 gctccccctg ggcatgccct ccctcccctt cgacgcgctc gtgttcgaga ccatggagtt
     1381 ggagggtacc gtcgagaagg ggatcgtgaa gatcgaccgg ggcgagttca caggcgagtt
     1441 ctcgggaaac ctcacgggca tggtgaggct cctcaaggac atgaggcgct cgaggctcaa
     1501 cctgacggga gaggtgagcc tgcccgaggc agtcagggcc tcactcggcg ccggctcgcc
     1561 cccccagggc cagcccgcga ggttcagcct cagggggacc atggagaggc cgaggttcag
     1621 gattgtcggg ctcacccagc cggggggcgc tccggcgagg ccggccgtcg cgggggcaag
     1681 ccagggccag gtctctgccg cggcgctgcg gcgcgagtcc gagcgctcac gcatcagggc
     1741 gggggccgag gtcacggcgc cgccggatgc gggtgtcggc ccgggtgaac aggcccttga
     1801 ccaggccgat ggagaagggt tcgacatgga aggggagtga aatggcatcg atgcacgagg
     1861 cgggtcggtt tccgaaggcg ttgaggctgt cgtggaacga ggtggaaggt ctcctgtggg
     1921 acatggcagg ctccatcaga agagacggct acgaccccga ggtcatcatc gccgtcgcga
     1981 ggggtggcct cgtgccggcg aggatactgg ccgactacct gcagaagaaa
//
LOCUS       DAOSCR010000515         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36695_length_2029_cov_3.083080, whole genome shotgun sequence.
ACCESSION   DAOSCR010000515 DAOSCR010000000
VERSION     DAOSCR010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36695_length_2029_cov_3.083080"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLT69_11030"
     CDS             <1..877
                     /locus_tag="PLT69_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ15018.1"
                     /translation="AAEKAASMGVAAIVGCDWSSHSLAAAPVAQARGIPMVSNVSTND
                     TLTSIGECIFRVCYTDRLQGKALALFASRDLKAHRALMLVDVSSDYAIGLSSEFSRGF
                     TGPDRTSMRRNYTMEGLDEAWLERVASFAKRYKPDLVFIPGYDESAVIAAHLSRRGVT
                     STFLGGDGWGSDAFYRKWHKPIQDAYFSSHWGMRQDTPASREFIKRYGSGRRRIADTE
                     ALAYDAVMLLADAVTRAGSGDRAKILAALAETRSYRGVTGLISFAEGRDPVKDVVMLA
                     IKDGVPRYHSTVNAR"
     gene            892..>2029
                     /locus_tag="PLT69_11035"
     CDS             892..>2029
                     /locus_tag="PLT69_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ15019.1"
                     /translation="MRGTRLPLRLVVVAVILSCLSPAELPGENRPLKVGLVMAKTGLA
                     TREEAPVLDAARLAVDLINTSGGIKGRTVELLVFDSASTPIGAKSAAQKAVSSGASCI
                     IGAFRSSHSLAMVPVADRASIPMISPASTNPEVTKGSPYAFRVCFVDSFQGAVMAGFA
                     SRELKAKSAVVIFNCNEDYSMTLARVFVDRFRELGGSILWEGSYTGNATDFKEILARV
                     RDLSPDVVYNPGYARDSGLFIGQAQAMGVKAVFLGADGWDQEIFPYAGGTIENAYSTT
                     HWHKDVPFKANERFKEAYRKRFGAFRVDNIRVPLTYDAIMLFADAARRARSLDGAAIR
                     EALATTRGFHGATGDITMGPDRTPVGKEASIMRSQGGSWRFHKAF"
BASE COUNT          378 a          633 c          675 g          343 t
ORIGIN      
        1 cgcggccgag aaggccgctt ccatgggtgt cgcggccatc gtgggctgtg actggagctc
       61 ccactccctg gcggccgcgc ccgtggcgca ggcccgcggc atccccatgg tctccaacgt
      121 ctcgacgaac gacacgctca cctcgatcgg cgagtgcatc ttcagggtct gctacacgga
      181 ccgtctccaa ggcaaggccc ttgcgctgtt cgcctcgcgg gatctgaagg cacacagggc
      241 gctcatgctc gtggacgtct cgagcgatta cgcgatcggc ctgtcttcgg aattcagccg
      301 gggcttcacc ggccctgacc gcacgtcgat gcgcaggaac tacaccatgg agggactgga
      361 tgaagcctgg ctggagaggg tcgcctcctt cgcgaagagg tacaaacccg atctcgtctt
      421 catcccgggc tacgacgaga gcgccgtcat agcggcgcat ctctctcgga gaggggtaac
      481 ctcgacattc ctgggcggag acgggtgggg atccgacgcc ttctacagga aatggcacaa
      541 gccgatacag gacgcctact tctcgagcca ctgggggatg cggcaggaca cgccggcatc
      601 ccgggaattc atcaagcgct acgggtccgg ccgcaggcgt attgccgaca ccgaggccct
      661 ggcctacgac gcggtcatgc tcttggccga cgccgtgacc agggcaggtt ccggcgacag
      721 ggccaagata ctcgcagcct tggccgagac caggtcctac cgtggggtca cgggcctgat
      781 ctcgttcgca gaggggaggg atccggtcaa ggacgtggtg atgctggcga tcaaagacgg
      841 ggtccccagg tatcactcga ccgtgaacgc gagatgaacg gggaggcctc tatgagggga
      901 acacgcttgc cgttgaggct cgttgtcgtc gcggtcatcc tgtcctgcct gagcccggcc
      961 gagcttccgg gggaaaaccg ccccctgaag gtcggcctcg tgatggccaa aaccggtttg
     1021 gccacccggg aggaagcgcc tgtgctcgat gcggccaggc tcgccgtcga cctgataaac
     1081 acctccgggg ggatcaaggg gaggaccgtg gagctcttgg tgttcgactc ggcaagcacg
     1141 cccataggcg cgaagtcggc ggcacagaag gccgtctcct cgggtgcctc gtgcatcatc
     1201 ggtgcgttca ggagttccca ttccctggcc atggtcccgg tggcggacag ggcgagcata
     1261 ccgatgatca gcccggcatc caccaacccc gaggtcacga aaggctcgcc gtacgccttc
     1321 agggtatgtt tcgtggactc gttccagggc gcggtcatgg ccggcttcgc ctcccgcgag
     1381 ctcaaggcga agagcgccgt cgtgatcttc aactgcaacg aagactacag catgaccttg
     1441 gcccgggtgt tcgtcgacag gttcagggag ctcggaggat cgatcctgtg ggagggctcc
     1501 tacacgggca acgccacgga tttcaaggag atcctcgcca gggtgaggga tctgtccccg
     1561 gatgtggtct acaacccggg gtacgctcgg gacagcggcc tcttcatcgg gcaggcccag
     1621 gccatgggcg tcaaggcggt tttcctcggg gctgacggat gggaccagga aatcttcccg
     1681 tatgcagggg ggacgattga gaacgcctac agcaccaccc actggcacaa ggacgtaccg
     1741 ttcaaggcga acgagaggtt caaggaggca taccgcaaga ggttcggcgc cttccgcgtc
     1801 gacaacatcc gcgtaccgct cacctacgac gcgatcatgc tctttgcaga cgcggccagg
     1861 agggctcggt ccctcgatgg agccgcgatc agggaagccc tggcaacgac gcgcggcttt
     1921 cacggggcca cgggcgacat aaccatgggg ccggacagga cccctgtcgg gaaggaagcg
     1981 agcatcatgc gctcacaggg cggctcctgg aggttccaca aggcctttt
//
LOCUS       DAOSCR010000516         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_36971_length_2018_cov_3.805400, whole genome shotgun sequence.
ACCESSION   DAOSCR010000516 DAOSCR010000000
VERSION     DAOSCR010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36971_length_2018_cov_3.805400"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..422
                     /locus_tag="PLT69_11040"
     tRNA            338..422
                     /locus_tag="PLT69_11040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:372..374,aa:Leu,seq:tag)
     gene            1125..1466
                     /locus_tag="PLT69_11045"
     CDS             1125..1466
                     /locus_tag="PLT69_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15020.1"
                     /translation="MEHVMKGRIQSVQFGRVQQFGNVSVFPLFLEARAVVVHLVLEKQ
                     VDEGAFKRRSRSCGRSSERKIPRERNCRKRSDEPGRREVMKRLFFMMLLVIAECRGED
                     PCDRCYHDDYH"
     gene            1663..>2018
                     /locus_tag="PLT69_11050"
     CDS             1663..>2018
                     /locus_tag="PLT69_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOJ15021.1"
                     /translation="MEKKLSALLDCGVCSVVNLMEVHEVDHYGRHFLDYSHRFVSLAL
                     KRGIEACVARFPIRDMGIPSRALMKDILDAIDANIAQGRPAYVHCWGGKGRTGTVVGC
                     YLARHGIATGSAALEM"
BASE COUNT          466 a          535 c          630 g          387 t
ORIGIN      
        1 ctcgcctaca ctcaccccga caggaatgag aacgccacac ggccccggcg tgctttagga
       61 ccccccttca ggaatgaggt cgccccacgg tctcaggcgt gtgaaagtgc gtctcgtcaa
      121 cacccccccc aggcaggaat gaggatgccc cgcgcggcaa accacaaggg gccgggcctc
      181 gatgcccatc gctgcaggtc gtcaaacacc tgttcgtgtc aagagggtcg tgacgagagt
      241 gtacccctcc aagcgtgaga caatggactg ggaggggagg gcgaggtcga gtgggtatgg
      301 acaagcccat gtccgtggat atactatccc tgctcgtgcg agagtggcgg aattggcaga
      361 cgcgctggac ttaggatcca gtggagcgat ccgtgggggt tcgagtccct tctctcgcac
      421 caatgatttc atcgatctgt tcggcggcac gtccatctcc tgccaccctg gatgacctga
      481 agtgtgcctg ttttatgccc gccctccgcc ttttttcagg acagccgcgt tcttctcatg
      541 gatgaaggag agggaggtgc agccatgttg aaggcgattc atgcgcaaga agaccatcgc
      601 aggcggccaa ataaggcggc acaggcgtca acccgtctga acgtctggac gtgtgaacgt
      661 gatgaagccg acttgagcgg ccaggctggt tatagacggc agagaagaga cgctgtcgta
      721 tatgtccttt ccccgggagt agtggctgag gatccggacg aacaatccca taggcccatg
      781 ctggtggtga agcagaaaga cggcggaggt atggccacca tagacctcag acaaagggat
      841 attgactaaa agaggaggat ctcctccgaa ggacagagac ctgaatcaaa agtgcgaaac
      901 attgttgaca ctatcatgcc cctcctttgg cctcccgggg tgatctcgtg gagcgtgccc
      961 atcgtgagcc atcaggaata gaccgtaaag gttgccttct aaaccatatt cattttttgc
     1021 gacataaagc tgtcgcggtg atcttacctg tatcgaaaaa ccggggtatg atcattttcc
     1081 cctggatggg tgccacaccc aggcggatgg agaagtaggc catcatggaa catgtcatga
     1141 agggcaggat tcagagcgta cagtttggca gggttcagca gttcgggaac gtgtcggtct
     1201 tccccctgtt cctggaggcc agggcagttg tggttcacct ggtcctcgag aagcaggtgg
     1261 atgagggggc gttcaaaaga agatccagga gctgcgggag gagctcagaa aggaagatcc
     1321 ctcgggaaag gaactgcagg aaaaggagtg atgagcccgg aagaagggag gtcatgaaac
     1381 gactgttctt catgatgctc cttgtcattg cggaatgcag aggtgaggat ccgtgcgacc
     1441 ggtgttatca cgacgattat cactaagagc acccgtatta ctgccaggag tgcctgcagg
     1501 agaaagacga caccgaagag gaggagaccc catgaaggtt gaggacacaa agagaaccca
     1561 ctcgcctgac gacatcacga aaagaccgtt cccccgctcg tactgggtcg tgccgggcat
     1621 gctctgcgcc ggcgaatacc ctgggacccg gatgcgaaga tcatggagaa gaagctctca
     1681 gctttgctcg actgcggggt ctgtagcgtg gtgaacctca tggaggtcca cgaggtggat
     1741 cactacggca ggcacttcct cgattacagc cacaggttcg tctctctggc cctcaagcgg
     1801 ggaatcgaag cgtgcgtggc gaggttcccc atcagggata tgggcatacc ctcccgggcg
     1861 ctcatgaagg acatactgga cgccatcgat gcgaacatcg cccagggcag gcctgcctat
     1921 gtccactgct ggggaggcaa gggccggacc ggcaccgttg tggggtgcta cctcgcgcgg
     1981 cacgggatcg cgacagggag cgctgccctc gaaatgat
//
LOCUS       DAOSCR010000517         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_37023_length_2016_cov_4.477307, whole genome shotgun sequence.
ACCESSION   DAOSCR010000517 DAOSCR010000000
VERSION     DAOSCR010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37023_length_2016_cov_4.477307"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLT69_11055"
     CDS             <1..278
                     /locus_tag="PLT69_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOJ15022.1"
                     /translation="GGRLVINAIRKEDADKGLLLGLNYAEHLWMEREIKSVANVTRAD
                     IADFLRIASSAGIVPEVETYPLSEANRALVELKRGPIRGAKVLVMDS"
     gene            436..969
                     /locus_tag="PLT69_11060"
     CDS             436..969
                     /locus_tag="PLT69_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15023.1"
                     /translation="MHRTESTRIIDEVLQKERREVAIFRRLASKVKTPLGKAVFMDYA
                     EKGRALCSSLEDLKRSHETGGEGSQVHGQGCVGTVRVVDHDDYEEKLSRAMEKALEKF
                     DDIRAIEVAARIVRKTARAISRSYEAVRDPAYRDALREIERAERENFMHLKNIEEYLK
                     DPQAWFREREHHHLDGA"
     gene            1773..>2016
                     /locus_tag="PLT69_11065"
     CDS             1773..>2016
                     /locus_tag="PLT69_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15024.1"
                     /translation="MAAERPDTGGEVIWRVSRIQPENHDVTSMFLEGKDAKFAARRAG
                     QYAVIRVMRPEGWSEPHPFTISCAPEADYLQFTIKAA"
BASE COUNT          421 a          567 c          677 g          351 t
ORIGIN      
        1 ctggagggag actcgtcata aacgccatcc gcaaggagga cgcggacaag gggctgctcc
       61 tgggcctgaa ctatgccgaa cacctctgga tggaacgcga gatcaagagc gttgcgaacg
      121 tgacaagggc ggacatcgct gacttcctga ggatcgcctc gtccgcaggg atcgtccccg
      181 aggtggagac ctacccgctg tccgaggcga accgggccct cgtggaactc aagaggggac
      241 ccatacgcgg cgccaaggtc ctcgtcatgg attcgtgaac ggtaatatgc caatacatta
      301 ccgattcaga ccgtgcgacc tgccggcagc cacctcaccc ttttgtttga gaaacgatgc
      361 ttcccttatg gtaagagtta ttgtatgata gcagtgcgtg attggaatga ctctgcggtg
      421 aaaggatggg tgtgtatgca tcggacggaa tcaaccagaa tcattgacga agtactccag
      481 aaagagcgca gggaagtggc gatcttcagg cgcctggcga gcaaggtcaa gacccccctc
      541 gggaaggcag tgtttatgga ttatgccgaa aaggggaggg cgttgtgctc cagcctcgaa
      601 gacctgaagc gatcgcacga gacgggggga gagggttcgc aggtgcacgg ccaagggtgc
      661 gtgggcacgg tgagggtcgt cgatcacgac gattacgagg agaagctctc ccgggccatg
      721 gagaaggccc tggagaagtt cgacgacatc agggccatcg aggtggcagc acggatcgtg
      781 cgaaagaccg cacgggccat ctcgcgctcg tacgaggcgg tcagggaccc cgcctaccgc
      841 gatgcgctca gggagattga gcgtgccgag agagagaact tcatgcacct gaagaacatc
      901 gaggaatacc tcaaggaccc ccaggcgtgg ttcagggagc gggaacacca ccacctcgac
      961 ggtgcatgat gctgcaggca tctcctgcag gtggacgagg ccgcaccggt gggggaggcc
     1021 tcgtcgttgc cagaggcaaa atgggtcagg gcaggcgtcg tcaagcccga gacgccacgc
     1081 ggttcgtgcc gcccttgcag ggcgtgctca ggcggccgaa caagaggaat cgcctcgttc
     1141 gcgacgccac gcaggaggtt gtgccgccct tcaaggcatg tggtgaggga cggtcttcgc
     1201 tcctggatgg atcttcccga accgaaaaag gccgggggca ggccggaaag gggaaggaag
     1261 tccctccggg ccgtcgaagg cctgtccacg agagaccggg tagcgtggct gaagaacgag
     1321 accacgtggg tatgacgagg gcctcccccg ttgtcgcccg tgccgcacgg tcacccttat
     1381 cgtctacgtg ttcacacgcg tcatggcgga tacctgcggg cgtggatacg gcctggacat
     1441 gtgcgtgccg cgcccgaggt tttctcgagc cgttgtatga gtgtcgatga tgtgtcggcc
     1501 ctgcggccga gccgtgccgt tcaagccggt tctcgtcaca cacggcgtca ggcatgcatg
     1561 cttgacgacc ttgagccacg gtaaacaccc tggtcaggca tcacgacatc acccatggcc
     1621 tcaggcgtgc aggcctgacg gccctcccgg ttttgattga atctcgtgcc cctgcaggat
     1681 ataacgggga gatacaggac agggtgcatg gcgtctcgcc tcctccatgt caccttgttc
     1741 ctgaactgcc acgtcgagtg aggggagggg atatggctgc ggaacgtcct gatacggggg
     1801 gcgaggtcat ctggcgtgtg tcgaggatcc agcccgagaa ccacgatgtg acatcgatgt
     1861 tcctcgaggg gaaagacgcc aagttcgctg ccaggcgcgc cggtcagtac gccgtgatca
     1921 gggtgatgag gcccgagggg tggagcgaac cgcacccgtt caccatctcg tgcgcacccg
     1981 aggcggacta tctccagttc accatcaagg cggccg
//
LOCUS       DAOSCR010000518         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_37081_length_2014_cov_3.681470, whole genome shotgun sequence.
ACCESSION   DAOSCR010000518 DAOSCR010000000
VERSION     DAOSCR010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37081_length_2014_cov_3.681470"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLT69_11070"
     CDS             complement(<1..722)
                     /locus_tag="PLT69_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOJ15025.1"
                     /translation="MALTEDMVKSLPRATGVYLLKDARGVPVYIGKANDVRSRVRSYL
                     AEDGRPFTSRIAGETARVDFILTRNESEALLLENQLIKKHRPRYNIDLKDDKSYVRIK
                     VSLSHPWPSISVTRTILKDGSRYFGPYASAQSTRRTLSALGRVFPLRRCKDTEFRNRK
                     RPCVYHSIGMCLAPCVRPHVRREYDQAVLDLIEFLEGKDRDLVENMRRRMLEAADRLD
                     FEEAARLRDRINALESTLVPQAV"
     gene            837..1415
                     /gene="pth"
                     /locus_tag="PLT69_11075"
     CDS             837..1415
                     /gene="pth"
                     /locus_tag="PLT69_11075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ15026.1"
                     /translation="MKLVYGLGNPGKRYALTRHNAGFMAVDVLCRRLKVSLRKTASGI
                     LYERARIKGVEVMLAKPMTYMNLSGEPFRTFRIDPQDLVVVHDDMDIPKGVVRVKVGG
                     GTGGHKGLDSLCTVLGSGDFVRVRLGIGRPPEGMDPSEFVLSPLDEAEKEAFEEQLEQ
                     AADAVIMCVTGETTRAMNTFNRRSEPRMDGPD"
     gene            complement(1474..1725)
                     /locus_tag="PLT69_11080"
     CDS             complement(1474..1725)
                     /locus_tag="PLT69_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15027.1"
                     /translation="MGKCFVVPEEVLNLTRLCPTEMGCLKDEAERSRPLCPVVFVVAE
                     HATVVDIRGDKRCPYAFDLGASGICSCPTRYAIYMKYGV"
BASE COUNT          427 a          588 c          630 g          369 t
ORIGIN      
        1 accgcctgcg gcacgagggt ggactcgagg gcgttgatgc ggtccctgag gcgggcggcc
       61 tcctcgaaat ccagcctatc agcggcttcg agcatgcggc gtctcatgtt ctccacgagg
      121 tctctgtcct ttccctcgag aaactcgatg agatccaaga cggcctggtc atactccctt
      181 cggacatgag gccgcacgca gggagccagg cacatgccta tggagtggta cacgcacggc
      241 ctcttcctgt tcctgaattc cgtgtccttg cacctcctca aggggaagac cctgcccagc
      301 gccgacaggg tacgcctggt cgactgtgcg gatgcgtacg gcccgaagta gcgcgaaccg
      361 tccttgagga tcgtcctcgt gaccgagatg gaaggccagg ggtgggagag ggagaccttg
      421 atgcgcacgt acgacttgtc gtccttgagg tcgatgttgt agcgaggcct gtgtttcttg
      481 atgagctggt tttcgaggag caacgcctcg gactcgttcc gggtgaggat gaagtcgacg
      541 cgcgccgtct ctccggcgat cctcgaggtg aaggggcggc cgtcttcggc caggtacgat
      601 ctcacccttg aacgcacatc gttcgccttc ccgatgtaga ccggcacccc acgcgcatcc
      661 ttgagaaggt atacgccggt ggcccgggga agggacttca ccatgtcctc tgtcagcgcc
      721 acgcctttat gatagcatgt cccggccgaa acggtgaaca attcaccttg acaggggatc
      781 ctatttgaag aggatggatg tggtataagg atgaacgaga agggacggac cggggcatga
      841 aactcgtcta tggtttgggc aatccaggga aacgctacgc gctcacccgt cacaacgcgg
      901 gtttcatggc tgtagacgtc ctctgcaggc gtctcaaggt ctcgctgaga aagaccgctt
      961 ccgggatcct gtacgagagg gcccgcatca agggcgtcga ggtgatgctc gccaaaccga
     1021 tgacctacat gaacctgagc ggcgagccgt tcaggacctt ccggatagac ccccaagacc
     1081 tggtcgtggt gcacgacgac atggacatac ccaaaggcgt ggtgagggtg aaggtggggg
     1141 gcggcacggg cgggcacaag gggctcgatt ccctttgcac cgttctcggc tcgggcgact
     1201 tcgtgagggt caggctcggg atcgggaggc ctcctgaagg gatggacccg tccgaattcg
     1261 tgctgtcgcc tctggatgag gcggagaaag aggccttcga ggaacagctc gagcaggcgg
     1321 cggacgccgt gatcatgtgc gtgaccggag aaacgacccg tgccatgaac acgttcaaca
     1381 gaaggtccga gccgcgcatg gacggccccg actgagaatg gccccgagct gaacgaaccc
     1441 aggaaaacgg cgcaagaggg gcacacaccc gacctagacg ccgtatttca tgtagatcgc
     1501 atacctcgtg gggcacgagc agatccccga ggcgcctagg tcaaaggcgt acgggcaccg
     1561 cttgtccccc cttatgtcga ccacggtcgc atgctcggcc acgacgaaca caaccggaca
     1621 aagcggccgc gagcgttcag cctcgtcctt gaggcagccc atctcggtcg ggcacagtct
     1681 tgtcaggttc agtacctcct cgggaacaac gaagcatttg cccatgtatc tgcctgcttt
     1741 cacatggtat tcagaggcgc accacgaatc gtgtgacccg aggcgcagcc ttacccattg
     1801 aacgttgccc cacccatggc ggcacacatg ccatgagact accaacaata cacctgtatt
     1861 gataaaatca ttaacaccat aacgctcaaa tggcaagaga tcgcaggcgg caagagatgg
     1921 atgccgtgac ggtcagagga cgcggggaag gcgtaccctg gcactcccta tcagaaggaa
     1981 acccctggca tggcacgtcc tgtgggtgag gagg
//
LOCUS       DAOSCR010000519         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_37281_length_2006_cov_3.912865, whole genome shotgun sequence.
ACCESSION   DAOSCR010000519 DAOSCR010000000
VERSION     DAOSCR010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37281_length_2006_cov_3.912865"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLT69_11085"
     CDS             <1..396
                     /locus_tag="PLT69_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ15028.1"
                     /translation="DCLGWAAYLTDWPGPAPGERPSAYIVILGDTVISRNFWCDHGIA
                     AQSILLGAVEKGYGGCMIASIKKQQLMQALGIPERYEILLVIAIGRPAERVTIEPVGP
                     QGDIRYWRDDQGVHHVPKRSLDEIVIERF"
     gene            543..1181
                     /locus_tag="PLT69_11090"
     CDS             543..1181
                     /locus_tag="PLT69_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ15029.1"
                     /translation="MDETDAAMTAGSDPGGRPRDPGGAAELADAARSGDRRAFERLVV
                     MYQEGIYRMAYARLQNVSDAQDVVQEVFLNAYRTIGTLRDPALIRAWLYRIALNRVRD
                     VVRRRKVLGFFGLGAQTEPEDHPHPGPDSFDRMASRAFWERLNAFLEALPAREREVFR
                     LRFVDELSIPEICSVLGKNESTVKTHLYRAVEKFRRTSFASGTGGDPSRGES"
     gene            1178..1708
                     /locus_tag="PLT69_11095"
     CDS             1178..1708
                     /locus_tag="PLT69_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15030.1"
                     /translation="MNEKSRGHLDPDRMAASVVDARELTVEEREHLEACAECSRQRER
                     LLSSLGALSRDAAGFTPGGPKRVVLPSEAYPRPMARLMSWSMGFAGAAALVVLAGVYL
                     LARIVTGPVQEADMSRLAMEAHQDKALLAEVQKLEDDPLPEPYAGIIPEPAGVPDDEF
                     FDFLIPPDAGPKGGKT"
     gene            1805..>2006
                     /locus_tag="PLT69_11100"
     CDS             1805..>2006
                     /locus_tag="PLT69_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15031.1"
                     /translation="MKRFMGTLLLWTTALVALSSPLFGQEVRPGRWWKDPHIVRELQL
                     GRHEQKALDDLYERNRGVLTETR"
BASE COUNT          353 a          613 c          729 g          311 t
ORIGIN      
        1 gactgcctgg gctgggccgc ctacctcacc gactggcccg ggccggcgcc cggggaacgt
       61 ccttccgcct acatcgtgat cctgggcgac accgtgatct cccggaactt ctggtgcgat
      121 cacggcatcg cggcgcagtc catcctcctc ggcgccgttg agaagggata cgggggctgc
      181 atgatagcct cgatcaagaa gcagcagctg atgcaggccc tcggcatccc cgagcgatac
      241 gagatccttc tcgtcatcgc gatcggcagg ccggccgaga gggtgacgat cgagcccgtg
      301 ggcccgcagg gtgacatacg gtactggcgc gacgaccagg gggtgcacca cgtgccgaaa
      361 cgttccctcg acgagatcgt gatcgagcgc ttctgagagg acggacgaag cgtccgagga
      421 cggcacgggg gctccttgag acgccgtcca ctcgggacgc ccgggcgtga gggggcgttg
      481 tagacttgcg ggacgcctcc tgcgtcacac atggtgagaa cactggggcc ttggacggag
      541 tcatggacga gacggatgcg gcgatgacgg ccggaagcga ccccggcggc aggccccgtg
      601 atcccggggg ggcggccgaa ctcgcggacg ccgcccggtc gggagacagg cgcgctttcg
      661 agaggctggt cgtcatgtac caggaaggca tataccggat ggcctacgcc aggctgcaaa
      721 acgtgagcga cgcccaggac gtcgttcagg aggtgttcct gaacgcgtac cggacgatcg
      781 gcaccctgag ggaccctgcg ctgataaggg cgtggctcta ccgcatagcg ctcaaccgcg
      841 tcagggacgt cgtgcgcagg cgcaaggtcc tcgggttctt cggcctgggc gctcaaacag
      901 agcccgaaga ccacccccat ccggggccgg actccttcga ccgcatggcg tcgcgtgcgt
      961 tctgggagcg cttgaacgcc ttcctcgagg cgttgcctgc ccgcgagagg gaggtcttca
     1021 ggctcaggtt cgtggacgag ctcagcatcc cggagatctg ttcggtcctg gggaagaacg
     1081 agagcacggt caagacccat ctctaccgtg ccgtggagaa gttcaggagg acgtctttcg
     1141 cttccggcac ggggggtgac ccatcgaggg gggagtcatg aacgaaaaga gccgggggca
     1201 cctggatccc gaccgcatgg cggcatcggt tgtcgatgcc cgggagctca ccgtcgagga
     1261 acgtgagcat ctcgaggcgt gcgccgagtg ctcccggcag agggagcgcc tgttgtcgag
     1321 cctgggcgcc ctgtcgaggg atgcggcggg gttcaccccc ggggggccca aacgcgtggt
     1381 gctcccctcg gaggcgtatc cgcgtcccat ggcgaggctc atgtcgtggt ccatggggtt
     1441 cgcgggggcc gcagctctcg tcgtgctggc aggggtgtac ctcctggcgc gcatcgtgac
     1501 gggcccggtg caggaggcgg acatgtcgag gctcgcgatg gaggcgcacc aggacaaggc
     1561 cctgctcgca gaggtgcaga agctcgagga cgatcccctt cccgagccat acgcggggat
     1621 catccccgag ccggcggggg tccctgacga cgagttcttc gatttcctca taccgcccga
     1681 tgcagggccc aaggggggaa agacgtgaag accgacgccg cttgtgtgag cccggcatgg
     1741 acttgccggt ggaccgcggc gaaacgaacg gggaaacaga catgaaggtc gagaggataa
     1801 ggccgtgaaa cggttcatgg gaacgctctt gctttggacg acggcgctgg tggcgctctc
     1861 ctcgccgctt ttcggccagg aggtgaggcc cggcaggtgg tggaaggacc cgcacatcgt
     1921 gcgggagctc caactcggca ggcacgagca gaaggccctg gacgatctct acgagcggaa
     1981 ccggggcgtc ctcaccgaga cgaggg
//
LOCUS       DAOSCR010000520         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_37366_length_2003_cov_2.980493, whole genome shotgun sequence.
ACCESSION   DAOSCR010000520 DAOSCR010000000
VERSION     DAOSCR010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37366_length_2003_cov_2.980493"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLT69_11105"
     CDS             complement(<1..850)
                     /locus_tag="PLT69_11105"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOJ15032.1"
                     /translation="MGDILETLKRYRAEGSRLVGCFPLYGPLELFHSFGLTPVVLWGL
                     GDGRGYYPASDAHVQGYACSVARALTGFVIEHGTALFDALFMYNACDTLRNLPEILSR
                     ALDSRGGHIPMFRLHLPAIPLDRAHAARYLEERLRGLVKALEEFTGRSFSGDAFMASA
                     TLYERRRSLCRGVMDLRARGGIPSARAARIIELGNHVEVAEHIAGLEEVVGSCSGLPG
                     DARGVRVMAGGIQAPPLELLDAMDSSNLVTVADDFATMARTHLASVSPRPDPHSYYTE
                     LYLHHPP"
     gene            1316..>2003
                     /locus_tag="PLT69_11110"
     CDS             1316..>2003
                     /locus_tag="PLT69_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOJ15033.1"
                     /translation="METRRVYFDNNATTPLHPEVKRVMADAMETFGNPSSLHAYGREA
                     RALVEEARKKVAAFIGAEPEEIMFVGSGSEGNNTVLSIVTCQGRPCSIGSFGPRNLVT
                     TKIEHPCILEASKCLAERGFGVTFVDVDSDGKVDLDRIEDAINDRTALVSVMMANNEI
                     GTVQDIKTIARICHDKGALFHTDAVQAVGKVRLDVKELDVDFLTLSGHKIYAPKGIGA
                     VYVRKGAPFCP"
BASE COUNT          345 a          645 c          688 g          325 t
ORIGIN      
        1 atggtgggtg atggaggtag agttccgtgt aatacgagtg cgggtccggc cgggggctga
       61 ccgatgcgag gtgggtgcgt gccatggtcg cgaagtcatc cgcgacggtc acgaggttcg
      121 acgagtccat ggcgtcgagg agctcgaggg gcggggcctg gatgccgccc gccatgaccc
      181 tgacccccct tgcatcgccg ggcagtccgc tgcacgaccc caccacctcc tcgagcccgg
      241 cgatgtgctc cgccacttcc acgtggttgc cgagctcgat gatgcgcgcg gcacgggcgg
      301 agggtatccc gccccgggcg cgaagatcca tgacccccct gcacagcgag cgcctccgct
      361 cgtacagggt cgcactcgcc atgaaggcat ccccggagaa cgatcttccc gtgaactcct
      421 cgagcgcctt cacgaggccg cgcaggcgtt cctcgaggta tcgggcggca tgcgccctgt
      481 ccagggggat ggccggaaga tgcagccgga acatggggat gtgtccgcct ctcgagtcga
      541 gggcgcgcga gaggatctcc gggaggttcc tcagcgtgtc gcatgcgttg tacatgaaga
      601 gtgcgtcgaa cagggcggtg ccgtgctcga tgacgaaccc cgtgagcgcc ctggccacgc
      661 tgcacgcata gccctgcacg tgggcgtcgc tcgccgggta gtacccgcgg ccgtccccca
      721 gcccccagag caccaccggc gtaaggccga acgagtggaa gagctcgagc ggcccgtaga
      781 gggggaagca ccccacgagc ctcgaccctt cagcccgata ccgtttgagc gtctcgagga
      841 tgtctcccac gtgtccctac ccgcgatgaa atcatggttg atcacgtcga atgtgatcgt
      901 tgccttgtca cgtgtccctg cccgcgagga aatcatacca ggtgtggttc tgtaagacaa
      961 tatgtcgagg atggcgccgc tccccgggct ggcgcacggc gctttcagcc gtggcgccgc
     1021 gtgtggcggc cctctcgcga tcgtccgcgg gcctgtcttg cccccgagag gcgcgtcgcg
     1081 gttcggcccg agcggcctcg tgccctggcc tctctccgtt ttcgcccgcc cggtaggcgt
     1141 cggcgcgcca tggggcatgg tcggcaacag cccgggcggc ctgtcccctc gagcggtcgg
     1201 gtttcacttg ccgtttgaca cgggcgcctg aaaagatgga tacactcatc aggcaccccc
     1261 cgggtccccg gagacgggac gcctgtggga gggcacgaaa gagacggggt tgcgcatgga
     1321 aacgagaagg gtgtacttcg acaacaacgc cacgacgcct ctgcaccccg aggtgaagcg
     1381 ggtgatggcc gatgccatgg agacgttcgg gaacccctcg agcctccatg cgtacggcag
     1441 agaggcgagg gcgctcgtgg aggaggcccg gaagaaggtc gccgcgttca tcggcgccga
     1501 gcccgaggag atcatgttcg tgggcagcgg ctccgagggc aacaacacgg tgctctccat
     1561 cgtcacgtgc caggggcgcc cgtgctccat cggctcgttc gggccgcgca acctcgtgac
     1621 gacgaagatc gaacacccct gcatcctcga ggcctcgaag tgccttgccg agcgggggtt
     1681 cggcgtcacg ttcgtggatg tggactccga cgggaaggtc gacctcgacc ggatcgagga
     1741 tgcgatcaac gacaggacgg ccctggtctc ggtgatgatg gccaacaacg agatcgggac
     1801 ggtccaggac atcaagacca tagcccgcat ctgccacgac aagggcgccc tcttccacac
     1861 ggacgcagtc caggcggtgg gcaaggtgag gctggatgtc aaggagctgg acgtggactt
     1921 cctcaccctc tcgggacaca agatatacgc ccccaagggg ataggcgcgg tatacgtgag
     1981 gaagggggcg ccgttctgcc cct
//
LOCUS       DAOSCR010000521         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_37734_length_1989_cov_3.115305, whole genome shotgun sequence.
ACCESSION   DAOSCR010000521 DAOSCR010000000
VERSION     DAOSCR010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37734_length_1989_cov_3.115305"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLT69_11115"
     CDS             complement(<1..355)
                     /locus_tag="PLT69_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ15034.1"
                     /translation="MKKIFLRLIPGDGIGVEVTRECRRILRVLEEIHGGVSFTLEEHP
                     WGCRYFLEKGVMMPRDGLSILSECDAILLGAVGYPGVPDHVSLQGLLLPIRQGFDQYV
                     NLRPIRLLEGLPSPLK"
     gene            complement(519..595)
                     /locus_tag="PLT69_11120"
     tRNA            complement(519..595)
                     /locus_tag="PLT69_11120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Arg,seq:acg)
     gene            complement(717..1139)
                     /gene="atpC"
                     /locus_tag="PLT69_11125"
     CDS             complement(717..1139)
                     /gene="atpC"
                     /locus_tag="PLT69_11125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOJ15035.1"
                     /translation="MAEEYIQVDVVTPEKAVLSRKAVEVVAPGSQGEFGVLIGHTPFL
                     TTLKPGQIIVRTEDRDIYLACGGGFAEVISDRVIILAETAEMAEDLKADELKEEVEQA
                     REKLRQLGKEDPDYEKWEKRLLRAEIKQRVLENWEKNR"
     gene            complement(1152..>1989)
                     /gene="atpD"
                     /locus_tag="PLT69_11130"
     CDS             complement(1152..>1989)
                     /gene="atpD"
                     /locus_tag="PLT69_11130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ15036.1"
                     /translation="GNDLWLEMKESGVIDKCALIYGQMNEPPGARARVGLSGLAVAEY
                     FRDEQNQDVLLFIDNIFRFTQAGMEVSALLGRMPSAVGYQPTLGTDMGELQERITTTT
                     KGSITSVQAIYVPADDLTDPAPATTFSHLDATTVLSRQIVELGIYPAVDPLDSTSRIL
                     DPKIVGVEHYTVARQVQQILQKYKELQDIIAILGMDELSEDDKLIVSRARRIQRFLSQ
                     SFHVAEQFTGMPGKYVELKDTIKGFKMIVNGELDDLPEQAFYMVGTIEEAIEKAKKLA
                     SD"
BASE COUNT          313 a          642 c          624 g          410 t
ORIGIN      
        1 ccttgagcgg cgagggcagc ccttcgagga gccgtatggg cctgaggttg acgtactggt
       61 cgaagccctg cctgatgggc aggagcagcc cctggaggga cacgtgatcg ggcacgccgg
      121 ggtaccccac ggccccgagc aggatcgcgt cgcactccga caggatcgac aggccgtcac
      181 ggggcatcat gacgcctttt tccaggaagt acctgcatcc ccacggatgt tcttccaggg
      241 tgaacgagac cccgccgtgg atctcctcga gcacgcggag gatcctcctg cactctcggg
      301 tgacctcgac gccgatgccg tcgcctggga tgagtctgag gaagatcttc ttcatgtctc
      361 ttcaccacgg acagggcgac cgtgtttctt ccaccgcgca acaaaggggg cggcacaaag
      421 gcggcgtaac gcgacaggcc cgcgttccgg ctgctcccgg cgcttcggga ccggggacga
      481 gcggggcgaa ggagtgggtc tctcgtgccc ttggtgtttg gtgcgcccag caagactcga
      541 acttgcgacc tttggattcg tagtccaacg ctctatccgg ctgagctatg ggcgcacgcc
      601 tcggttcgaa ctctcacgct tatagagaga ttctcctgcg gagtcaatac gcaccgaata
      661 caaggggagg ggccgcgtga ggcccctccc ctgaaccttg cgatcgggac gaccttctac
      721 ctgttcttct cccagttctc gagaacccgc tgcttgatct cggccctgag gaggcgtttt
      781 tcccacttct cgtagtcagg gtcttccttg ccgagctggc gcagcttctc cctggcctgc
      841 tcgacctcct ccttcagctc gtcggccttg aggtcttcgg ccatctcggc tgtctcggcg
      901 aggatgatca cgcggtcgga gatgacctcg gcgaaaccgc ccccgcaggc gagatagata
      961 tccctgtcct ctgtccggac gatgatctgg cctggcttga gcgtggtgag gaagggcgtg
     1021 tgtccgatga gcaccccgaa ctcgccctgg ctgccgggcg cgaccacctc cacggccttg
     1081 cggctgagca ccgccttctc cggggtgacc acgtccacct gaatgtattc ttcggccatg
     1141 ccgtctctcc cttagtctga agccagcttc ttcgccttct cgatcgcctc ttctatggtg
     1201 ccgaccatgt agaacgcctg ctcaggcagg tcgtcgagtt ctccgttgac gatcatcttg
     1261 aagcccttga tggtgtcctt cagctcgacg tacttgccgg gcattcccgt gaactgctcc
     1321 gccacgtgga aggactgcga caggaaccgc tggatcctgc gggcgcgcga cacgatgagc
     1381 ttgtcgtcct cggagagctc gtccatgccg aggatcgcga tgatgtcctg gagttccttg
     1441 tacttctgca ggatctgctg gacctggcgg gccaccgtgt agtgctcgac gccgacgatc
     1501 ttcgggtcga ggatgcgcga ggtcgagtcc agcgggtcca cggccgggta gatgcccagc
     1561 tccacgatct gcctcgagag aaccgtcgtg gcgtcgaggt ggctgaacgt ggtcgctggc
     1621 gccgggtcgg tcaggtcgtc tgcgggcacg tagatggcct gcaccgaggt gatggacccc
     1681 ttcgtcgtcg tggtgatgcg ctcctggagc tcgcccatgt cggtgcccag cgtcggctgg
     1741 tatcccaccg ccgagggcat gcggccgagc agagcggaga cctccattcc cgcctgggtg
     1801 aaccggaata tgttgtcgat gaagaggagc acgtcctggt tctgctcgtc gcggaagtac
     1861 tcggccaccg ccagccccga gaggcccacc ctcgccctgg ctccgggggg ctcgttcatc
     1921 tgcccgtaga tgagcgcgca cttgtcgatg acgcccgatt ccttcatctc gagccacaag
     1981 tcgttgccc
//
LOCUS       DAOSCR010000522         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38152_length_1974_cov_2.940073, whole genome shotgun sequence.
ACCESSION   DAOSCR010000522 DAOSCR010000000
VERSION     DAOSCR010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38152_length_1974_cov_2.940073"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1522
                     /locus_tag="PLT69_11135"
     CDS             62..1522
                     /locus_tag="PLT69_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ15037.1"
                     /translation="MGLPIYKDTKVVGCLELFRKGSEAFTFEDLILTKHLLLSSERVI
                     REVLSSEREYDEVLDVTLDVPQRHVLHQYEELARRMGFPLAVALIEIDDPERFGLYQH
                     IPEGIRTIKNLAKRIQEGLRCYDKVLRYEELSFFVILPGCSPTEAVTALHKASLRLGA
                     DLADTMTIAVATMPDDAQDAKGIIHVAHQVLSHARKKGLHIATYAQTEMVRHVNSSLE
                     LRLKRILHAGPCSTALDELLDLVKIQCQADDVCLRYEPPGKLITWEGNELGYIQHAGL
                     SQEICDWVATCIAPAWAFVLGMNTDVSDWYYGVLSTASLLSDLRAGCPMGYSLKISDE
                     VYALAREMGVGDVIASQWATSSLVANIGYLGIPTTLFTKGELTPFDRKKITMHPVITT
                     RILKDVAVLDLDGDLLSSHHENFDGTGYPRGLRGEDIPLSARAMRVVDSFNAMVTPRL
                     HRPKIPFKEALRELCTMSGKSLDPEIVSLYVDVIGS"
     gene            complement(1581..>1974)
                     /locus_tag="PLT69_11140"
     CDS             complement(1581..>1974)
                     /locus_tag="PLT69_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ15038.1"
                     /translation="REFNKPRMEIAPDALRALIEYDWPGNVREIRNCIERAVLLAEGD
                     LIRLQDISILRPNNAVAISANETPSAQNQKNLSTLALTEREMILDALRKNDWVQKDAA
                     AMLGISKRVIHYKIKKYGITHPRWIKNR"
BASE COUNT          368 a          623 c          611 g          372 t
ORIGIN      
        1 caaagacgtg tcctagtgcg gcgtagaccc ccgtgacctg cccatccatt gcacctcggt
       61 gatggggctc cccatctaca aggacacgaa ggtcgtgggg tgcctcgagc tgttcaggaa
      121 gggatccgaa gcgttcacgt ttgaggatct catcctgacc aagcacctgc tgttgagctc
      181 cgagagggtc atacgggagg tactctcttc cgagagggag tacgacgagg tgctcgacgt
      241 cacgctcgat gtaccccagc gccatgtact ccaccagtac gaggagctgg cgaggcgcat
      301 gggcttcccg ctggccgtcg ccctgataga gatagacgac ccggagcgtt tcggtctcta
      361 tcagcacata cccgagggca taaggacgat aaagaacctc gccaagagga tacaggaggg
      421 gcttcgctgc tacgacaagg tcctgcgcta cgaggaactg agcttcttcg tgatcttacc
      481 cgggtgcagc cccaccgagg ccgtcacggc cctgcacaag gcctctctga ggctcggggc
      541 cgatctcgcg gacacgatga ccattgcggt cgccaccatg cctgacgacg cccaggacgc
      601 caaggggatc atccacgtgg cgcaccaggt gctctcgcac gccaggaaga agggcctcca
      661 catagcgacc tatgcacaga ccgagatggt caggcacgtg aacagctccc tggaactcag
      721 gttgaagcgg atccttcacg cagggccctg ctcgacggcg ctggacgaac tgctggacct
      781 cgtcaagatc cagtgccagg cggacgatgt ctgcctcagg tacgaaccgc ccgggaagct
      841 catcacctgg gaaggcaacg agctcggcta catccagcat gccgggctct cccaggagat
      901 atgcgactgg gtggccacgt gcatagcccc tgcatgggcg ttcgtgctgg gcatgaacac
      961 cgatgtctcc gactggtact atggggttct cagcacggcc tccctcctga gcgacttgag
     1021 agccggctgt cccatggggt attccctcaa gatctccgac gaggtctacg ccctggcacg
     1081 cgagatgggg gtgggggacg tgatcgcatc ccaatgggcg acctcgtccc tggtggcgaa
     1141 catcggttac ctcggtatac cgaccaccct gttcaccaag ggggagctca cgcccttcga
     1201 caggaagaag atcacgatgc accccgtgat cacgacccgc atcctcaagg acgtggcggt
     1261 tctcgacctc gacggcgacc tcctctccag ccaccacgag aacttcgacg ggaccgggta
     1321 ccccaggggg ctcaggggag aggacatacc gctctcggcc cgggccatgc gcgtcgtcga
     1381 ttccttcaac gccatggtaa ccccgaggct tcaccggccg aagataccct tcaaggaagc
     1441 gctcagggaa ctctgcacca tgtcgggcaa gagcctggac cctgagatcg tctcgctcta
     1501 cgtggacgtt atcggctctt gaaccggccg ggtgacgggc aggggcggcc tcgcgagcgg
     1561 ccccacggct cgcgtggacg tcatcggttc ttgatccagc gcgggtgcgt gatcccgtac
     1621 ttcttgatct tgtagtggat caccctcttg ctgatgccga gcatggcggc agcgtccttc
     1681 tgcacccagt cgttcttgcg cagcgcatcc aggatcattt ccctctcggt gagcgcgagg
     1741 gtcgacaggt tcttctggtt ctgggcggag ggtgtctcgt tcgcggatat ggcaacggcg
     1801 ttgttcggtc tcaggatgga gatgtcctgc agcctgatga ggtcgccctc ggcgagcagg
     1861 accgcccgct ctatgcagtt cctgatctcc cggacgttgc ccggccagtc gtactcgatg
     1921 agggccctga gtgcgtccgg ggcgatctcc atccggggct tgttgaactc cctg
//
LOCUS       DAOSCR010000523         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38207_length_1972_cov_3.102765, whole genome shotgun sequence.
ACCESSION   DAOSCR010000523 DAOSCR010000000
VERSION     DAOSCR010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38207_length_1972_cov_3.102765"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            646..1332
                     /gene="pncA"
                     /locus_tag="PLT69_11145"
     CDS             646..1332
                     /gene="pncA"
                     /locus_tag="PLT69_11145"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOJ15039.1"
                     /translation="MADRTASVAIPVGLEGHDALIVVDVQNDFMPGGALAVPGGDEIV
                     PGVNALMERFADEGLTVVLTQDWHPAGHRSFASAHRGKRPFDPHEEPGVGPVLWPDHC
                     VQESLGADFHPALRTGLAHVVLRKGTRRGVDSYSGFLENDRATPTGLAGCLKDRGVKR
                     IFVCGLALDYCVFFTAADRVRLGFEVFVLAELCRAVSPRPEDRSRIMDEMASIGIRVS
                     VDTKGGGGQT"
     gene            complement(1719..>1972)
                     /locus_tag="PLT69_11150"
     CDS             complement(1719..>1972)
                     /locus_tag="PLT69_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15040.1"
                     /translation="RFEECTEAISCVVTDQTMPGITGAELASQLLRLRPGLPVILMTG
                     FSELIGRDEALAMGIREYLDKPFTMGVLARAVKRCLTGG"
BASE COUNT          311 a          589 c          717 g          355 t
ORIGIN      
        1 tcgtgaaccg catagagttt tgccagatcc atgccacgtc cggacaggtt cttcccgggg
       61 gtctcgagag cgcatacatg ctgtactatc ggggagatgg gggcgccctt tcctcgtgag
      121 gaccatggcg gtcatccgta attatggttt ctggaccata aactatggcg ggtgcgcact
      181 atggtcgggg caccacttcg gtggcggcct tcatgccggt gcgcgatgag acgggctgga
      241 cggtggactc aacggccttg tgtccaggcc gttgaccggc tttacaggcc agggcgcggc
      301 cctgggcaag agggggatcg cgtggtcttt gttccacgag aggcagggcc ccggcccgtg
      361 tcgaggccgc ccaaacagtg gtgttcctgt cgcattgcgc ctcgcggcgc ttccgggccg
      421 ggaggtcgag gccgcaaggg gaacggccgg catgaagagg cgtgtgaccc tcggaggaaa
      481 acgggtgacg gccgtgtcct gcgtgatgcc cgggcattcg cacgccccca cccacgaaca
      541 gggacatccc gacggggggc ctgttgtccc gaggcgcttg cgctgaatgg ccctcagctg
      601 gtagcatcgc gtctgcgagg actctcgtct ccggaggttg gggccatggc cgataggaca
      661 gcgagcgtgg cgatcccggt gggtctcgaa gggcatgacg ccctcatcgt ggtcgatgtg
      721 cagaacgact tcatgccggg aggcgccttg gcagtgcccg gcggggacga gatcgtgccc
      781 ggggtgaacg ccttgatgga gcgtttcgcg gacgagggcc tcaccgtggt gctcacgcag
      841 gactggcacc ctgcagggca ccggtcgttt gcgtcggcgc acagagggaa acgccccttc
      901 gatccccacg aggagccggg tgtcggcccg gtgctctggc cggaccattg cgttcaagag
      961 agcctcggcg ccgactttca cccggcgttg cgcaccggtc tcgcccatgt cgtcctgagg
     1021 aaggggacga gaaggggtgt ggacagttat tccggtttcc tggagaacga ccgggccacc
     1081 cccacaggcc tcgccggctg cctgaaggac aggggggtca agaggatctt cgtgtgcggg
     1141 ctcgccctgg attactgcgt cttcttcact gccgcagaca gggtgcgcct cggcttcgag
     1201 gtgttcgtcc tggccgagct gtgccgggcc gtgagtccca ggcctgaaga tcgaagccgc
     1261 atcatggacg agatggcgtc gatagggata agggtgtccg tggacacaaa gggcggcggg
     1321 gggcagacct gaggatcatg gcgtcgccgg aaaaggaccc gccatccact tgcgatccgc
     1381 agggttgttc ttcgccgggc tgtgcgctcg ggaagcccga tctcgaatgg ggcgcgtaat
     1441 cggcgaggtc tcgctggatg ggcgtgagac acctggccgc ggcgtgagcc ccttgcatgg
     1501 aaggcccgcg ctcgccccgc tccgggtatg ggtgcagctg ggaagcggcc gtgccggtag
     1561 atgacggccg actggtcttt gtctcccctc gacatgagcg ggcgccgtct cgggatgggg
     1621 gatgcttggt gcccgacccc agggtggacg agggagcaag gcggcatgag ccggggacct
     1681 ctcgaggtgg aggatccttc cggacgtctc ccctggcgtc acccaccggt gaggcatctc
     1741 ttgacggccc gagcgagcac gcccatggtg aagggcttgt cgaggtactc tctgatgccc
     1801 atggcgagcg cctcgtcgcg gccgatgagt tcgctgaaac cggtcatgag gatcacgggc
     1861 agtcccggcc tgagcctcaa cagctgcgag gcgagctctg cgccggtgat cccgggcatg
     1921 gtctggtcgg tgaccacgca ggagatcgcc tcggtgcatt cctcgaacct gt
//
LOCUS       DAOSCR010000524         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38574_length_1960_cov_2.287664, whole genome shotgun sequence.
ACCESSION   DAOSCR010000524 DAOSCR010000000
VERSION     DAOSCR010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38574_length_1960_cov_2.287664"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..600)
                     /locus_tag="PLT69_11155"
     CDS             complement(61..600)
                     /locus_tag="PLT69_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOJ15041.1"
                     /translation="MGRSARRTLAAGAAVVLALCVGAYVFAGASRARLVSHVYDGDTL
                     RLADGTKVRLIGVDAPEVESPYGRGEPYGEESRKFLASLVEGRKVSLTIGDPPVDRYG
                     RTLAYVYVGNVLVNGRIIREGWAEAYRRFPHPMRDLFVAYEREARSKGLGIWAGRRAK
                     QKAGPGGVLRGAGTGGLAQ"
     gene            complement(600..1727)
                     /locus_tag="PLT69_11160"
     CDS             complement(600..1727)
                     /locus_tag="PLT69_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15042.1"
                     /translation="MKTRKDVHPAQAGSRSRPAKTPVEKLFVTFNGETVRGYVLAIDA
                     RTGREKEAASSGNAQPPPVIVFFPGHAQRPDDAFEFTGRLAWKSRSGIVIVPVCDTPH
                     GDDPSLRGDDGKVTVLVELVRHACAGLGLGIHGQAPQTGAMAVALGPPAPPLPPGSPR
                     VMLAVVGWSHGAILARRVAHAYPGTVVSLAQVCPAGYEATTPGKLTARFVRESASMVA
                     RAGGEGRMGKLARSAWGFTKGLCGDFARSIALAGTSLDPSKVLRVFRDIHDCTTLCDS
                     TRFKASHLRRIAVFFGASDTLMDPIRLLGTAEVGDPCKEDLERFRRRFFADVPDSATI
                     RLGILPGTHAAPVTHAETYADAVLEGIGEGPGKVGEAGGGR"
BASE COUNT          301 a          713 c          633 g          313 t
ORIGIN      
        1 cctctgggtg gcgtcgaggc tcaggaacag gtatgcctga ggtgtccgcc tcaccggggg
       61 tcattgggca agacccccgg tgcctgcacc gcgcaagacg ccgccggggc cggccttctg
      121 cttcgcgcgc ctgccggccc atatcccgag acccttcgac ctcgcctccc tctcgtaggc
      181 cacgaagagg tccctcatgg gatgcgggaa tctcctgtac gcctcggccc acccctcgcg
      241 gatgatccgc ccgttgacga ggacattgcc cacgtagacg taggccaacg tcctgccgta
      301 cctgtccacc ggggggtctc ctatggtcag ggacaccttc ctgccctcga cgagagaggc
      361 gaggaacttc ctgctctcct cgccgtaggg ttcacccctc ccgtaggggg actcgacctc
      421 gggcgcatcc acacctatga ggcggacctt cgtcccgtct gcaagacgca gggtgtcccc
      481 gtcgtacacg tggctgacga gacgggccct cgacgcgcct gcgaacacgt atgcgccgac
      541 gcacagcgcg aggacgactg ccgctcccgc cgcgagggtg cgcctggcgg accgccccat
      601 cacctcccac ccccagcttc acccaccttt cccggacctt caccgatccc ctcgaggacc
      661 gcatccgcgt aggtctccgc gtgggtgacc ggcgccgcgt gggtgccggg caggatcccg
      721 agacgtatcg tggccgaatc cggcacgtcc gcgaagaacc tccgcctgaa gcgctcgagg
      781 tcttccttgc agggatcccc cacctcggcc gtgcccagga ggcgtatcgg gtccatgagg
      841 gtgtccgagg ccccgaagaa gacggcgatg cgcctgaggt gcgaggcctt gaaccgcgtg
      901 ctgtcgcaca gggtggtaca atcatgtatg tccctgaaga ccctgagcac cttcgaggga
      961 tccagggagg ttcccgcaag cgcgattgac ctcgcgaaat ccccgcacag gcccttcgtg
     1021 aacccccatg cagaccgggc gagcttgccc atgcggccct cacccccggc ccgggcgacc
     1081 atggatgccg actcccggac gaacctcgcc gtgagcttcc ccggggtggt ggcctcgtag
     1141 ccggccgggc acacctgtgc gagcgacacc acggtacccg ggtacgcgtg cgcgaccctc
     1201 ctcgccagta tcgcaccgtg ggaccacccc accacggcga gcatcacgcg tggggatccg
     1261 ggggggagcg ggggagcggg cgggcccagg gcgacggcca tggcgccggt ctgcggcgcc
     1321 tgcccgtgga tcccgagccc caggccggcg caggcgtgcc tgacgagttc cacgagcacc
     1381 gtgaccttcc cgtcgtcgcc cctcagggaa gggtcgtcac cgtggggcgt gtcgcacacc
     1441 gggacaatca cgatgcccga cctgctcttc caggcgagcc ttcccgtgaa ctcgaaggcg
     1501 tcatcgggcc tctgcgcgtg cccggggaag aagacgatca ccgggggcgg ctgggcgttg
     1561 cccgaggacg cggcctcctt ctcccggccg gtccttgcgt cgatggcgag cacgtacccc
     1621 ctgacggtct cgccgttgaa cgtgacaaag agcttctcga caggggtctt agccggtctt
     1681 gaccgagacc cggcctgggc cgggtgcacg tcttttctcg tcttcatgca tctccccgta
     1741 tcgccgatat cccgtgggcc cgtcgggtag gtccgacgcc agggaaccgc acgggggttc
     1801 atgacgcacg aatatcgtgg tcatcgaagc cccggcgtcc gggagtcatt ataacccccg
     1861 acggtctcct tccgcaaagg gtacgaggcg tcggcccccg tgaacgaggc gcctgcgcat
     1921 gccgccgcat ggccggtgca tgcccgtgat ccccggatgc
//
LOCUS       DAOSCR010000525         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38642_length_1957_cov_4.161409, whole genome shotgun sequence.
ACCESSION   DAOSCR010000525 DAOSCR010000000
VERSION     DAOSCR010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38642_length_1957_cov_4.161409"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLT69_11165"
     CDS             complement(<1..840)
                     /locus_tag="PLT69_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ15043.1"
                     /translation="MSERKLIVLGTASQVPNRVRNQNGYFLRFDDEGFLFDPGEGTQR
                     QMLLAGIPASAVTKIFITHFHGDHCLGLAGVIQRISLDRVPHEVEVFYPASGQVFFER
                     LQNACIYFPAARIHARAVCDEGVVHEDAKMTVRAFRLDHTVDTFGYRIDERDSFTLDP
                     ARLAQAGIAGAEAALLKSRGSLEKDGRVFKVEELGTLRRGQRFAFVMDTRVCEAAHGL
                     AAGADLCVMEATYLSDMERAASEYGHLTASQAGRIAKASGVKRLVLTHYSQRYTDTDG
                     FLRE"
     gene            1019..>1957
                     /locus_tag="PLT69_11170"
     CDS             1019..>1957
                     /locus_tag="PLT69_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15044.1"
                     /translation="MPDYTLDKPDNLVDFLEESVAAYPSSPFLGTKKAPGRYEWITYR
                     DFGARVDRVRAGLASLGVKKGDAVGIIANNRTEWAICAYATYGLVARWVPMYEAELPS
                     TWRYIVSDSGIKVLFCSNRSVMDKVKDFPSTIPSLERLILIEGEGPGSLQELEEIGAK
                     NPVPSTKPDPDDVAALIYTSGTTGDPKGVLLTHGNFTSNSLAGQKFYPEFKHGDVLLS
                     ILPWAHSYAQTAELYTVTRLGAQLGIMGGIDTLAEDMLAVRPTFLIAVPRVFNKIYDG
                     IFARMGQEKGLKKKLFFMAVESAKKRRMLKAEGRRDP"
BASE COUNT          365 a          652 c          614 g          326 t
ORIGIN      
        1 ctccctgagg aatccgtcgg tgtccgtgta gcgctgggag tagtgcgtga gcaccagcct
       61 cttcacgccg gaggcctttg cgatgcggcc ggcctgggag gcggtgaggt gcccgtactc
      121 cgaggccgca cgctccatgt cggagaggta cgtggcctcc atgacgcaca gatcggcccc
      181 cgccgcgaga ccatgggcgg cctcgcacac cctcgtgtcc atcacgaacg cgaacctctg
      241 gcccctgcgc aaggtgccga gttcctcgac cttgaacacc ctgccgtctt tttccagcga
      301 ccctctggac ttgaggagcg ccgcttcggc gcctgctatc ccggcttgtg ccagcctggc
      361 ggggtcgagc gtgaacgagt ccctctcgtc tatgcggtac ccgaaggtat ccaccgtgtg
      421 atcgagcctg aaggccctta ccgtcatctt cgcgtcctcg tgcacgacgc cctcgtcgca
      481 taccgcccgg gcgtggatgc gtgccgcagg gaagtagatg caggcgtttt ggagcctctc
      541 gaagaacacc tggccagagg ccgggtagaa gacctccacc tcgtgaggca ccctgtcgag
      601 ggagatgcgc tggatgacgc ctgcgagacc caggcagtgg tcgccgtgga agtgggtgat
      661 gaagatcttg gtgacggcgc tcgcggggat gccggccagg agcatctgtc gctgcgtgcc
      721 ctcaccgggg tcgaagagga acccctcgtc gtcgaaacgg agaaagtacc cgttctggtt
      781 ccttacgcgg ttgggcacct ggctcgccgt ccccagcacg atgagcttgc gttcggacat
      841 gacccgatca ccttcccaca aacaccggcc gagcgcaagc gcgcagggcc cccggcccgg
      901 gcttcgctcg aggtgcatgg gggggtcggg gatcaaggta cgatcgaaaa tgtacttgac
      961 acccactcct cggcgttgtg tatatccagc acccacgaaa ctcagccagg gaggaaccat
     1021 gcccgactac acgctcgaca aaccggacaa cctcgtcgac ttcctcgagg aaagcgtagc
     1081 cgcataccct tcgagcccct tcctgggaac caagaaggcc ccgggccgct acgagtggat
     1141 aacataccgt gatttcggcg cgagggtcga cagggtcagg gcgggcctcg cctccctggg
     1201 ggtcaagaag ggcgacgcgg tgggcatcat cgccaacaac cgcaccgagt gggccatctg
     1261 cgcgtacgcg acctacgggc tggtggcgcg ctgggttccc atgtacgagg cggaactccc
     1321 gtccacgtgg agatacatcg tctcggacag cggcatcaag gttctcttct gttcaaaccg
     1381 gtccgtcatg gacaaggtga aggacttccc gagcaccatc ccgagcctcg agaggctcat
     1441 cctcatcgag ggtgagggcc ccggctcact ccaggagctc gaggagatcg gcgcgaaaaa
     1501 ccctgtgccc tccacgaagc ccgacccgga cgacgtggcc gccctcatct acacctccgg
     1561 gaccacgggc gaccccaagg gcgtgctcct cacccacggc aacttcacga gcaactctct
     1621 ggcaggccag aagttctacc ccgagttcaa gcacggggac gtgctgctca gcatccttcc
     1681 gtgggcccac tcgtacgccc agacggccga gctctacacg gtcacccgcc tcggcgctca
     1741 gctcggcatc atggggggca tcgacaccct cgccgaggac atgctcgcgg tgaggcccac
     1801 cttcctcatc gcggtgcccc gcgtgttcaa caagatctac gacggcatct tcgcgcgcat
     1861 gggccaggag aaaggcctca agaagaagct cttcttcatg gcggtggaga gcgcgaagaa
     1921 gaggcgcatg ctcaaggccg agggcaggag ggacccg
//
LOCUS       DAOSCR010000526         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38644_length_1957_cov_3.982124, whole genome shotgun sequence.
ACCESSION   DAOSCR010000526 DAOSCR010000000
VERSION     DAOSCR010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38644_length_1957_cov_3.982124"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLT69_11175"
     rRNA            complement(<1..362)
                     /locus_tag="PLT69_11175"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(698..1891)
                     /locus_tag="PLT69_11180"
     CDS             complement(698..1891)
                     /locus_tag="PLT69_11180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ15045.1"
                     /translation="MSATIVNSMVIAAVLWNVIDRQRVATWCAVNVLYALSRYAIIFF
                     YRRRFAPAHLRIWRAGTYVSFAVAGILFGSSGLFLMDPAQIEYDIFLYFVAGGMVVGS
                     AGSYHNELPIYFIYSATVFLLPTVGIYLMDLEISKPMIVLGAVFYALVSFMAMRLSRD
                     LRDSLLLRYDNIQLVRRLEEEKANTERLNEQLVEKNRELNELSLVDPLTSLRNRRYLF
                     EIVMPEVEAFGRSDRRTRSMGDNRTSGGLKGYGIFVVDIDHFKQVNDTYGHDSGDMVL
                     VQFADRLRESVRSDDVVSRFGGEEFVVVLKQLQEDHAAGIARKLHGHIRTSTFTISRG
                     RTLNLTCSMGFVFFPLRGSTNREVTFDELISMADGALYHAKSQGRNACVQAVCNGESV
                     DYVLL"
BASE COUNT          378 a          616 c          555 g          408 t
ORIGIN      
        1 ctcccgtagg agtctggacc gtgtctcagt tccagtgtgg ctggtcgtcc tctcaaacca
       61 gctatggatc gtcgccttgg tgagccgtta cctcaccaac tagctaatcc aacgcgggcc
      121 catccaagag cgatagcttg catgcagagg ccatctttcc ccggcagact tgtgtctgcc
      181 ggacgtatgc gggattagca cgcctttcgg cgagttatac cccactccag ggcaggttac
      241 ccacgcgtta ctcacccgtg cgccgcttta ctcgcccccc gaaggggact ttctcgctcg
      301 acttgcatgt gttaggcacg ccgccagcgt tcattctgag ccgggatcaa actcttaagt
      361 ttaatctcgt ggagcgtttg cacgctgcct accttccact attcagtttt caaagatcga
      421 ttgcgcacga cggcaacttt acactatgac accgttttca tcggctgtca agatcttttt
      481 gaaagaaccg cgtgacgctc ggcggattgg ttatctagca accttgccac cgcgtgtcaa
      541 gggggttttt tctccccctc ccgacaccct cgagctccgg cttgcgccgt gcaagagcct
      601 gcaccttcta tgcgaaaacc ggaggggctg tcaagggggg aaatcttcac cctctcatga
      661 aggggctcac gccgctcgaa ggccgcacct gcacgggcta caggagcacg tagtcaacgg
      721 attccccgtt acagaccgcc tgcacgcagg cgtttctccc ctgagacttg gcatggtaga
      781 gggcgccgtc cgccatggat atcagctcgt cgaaggtcac ttcgcggttt gtgctgccgc
      841 gcaggggaaa gaacacgaaa cccatggagc aggtgaggtt caaggtcctg cccctactga
      901 tcgtgaaggt ggatgtccgg atgtgcccgt gcagcttcct cgcgatgccc gcggcatggt
      961 cctcctggag ctgcttgagg accacgacga actcctcgcc cccgaagcgg ctcaccacgt
     1021 cgtcgctcct gaccgactcc ctcagcctgt cggcgaactg gacgagcacc atgtcccccg
     1081 agtcgtggcc gtaggtgtcg ttcacctgct tgaagtgatc gatgtccacg acgaagatcc
     1141 cgtacccctt caggccgccc gaagtcctgt tgtcacccat gctcctggtc ctccggtcgc
     1201 tcctgccgaa ggcctccacc tcgggcatga cgatctcgaa caggtaccgc ctgttcctca
     1261 gcgacgtcag cgggtcgacc agcgacagct cgttcagttc cctgttcttc tccacgagct
     1321 gctcgttgag ccgttcggtg ttggccttct cttcctccag ccttcgcacg agctggatat
     1381 tgtcgtagcg caacaggagc gagtcccgaa ggtcgcggct cagccgcatg gccatgaagg
     1441 atacgagggc gtagaacacg gcgccgagga cgatcatggg cttggagatc tccaggtcca
     1501 tcaggtagat gcccacggta ggcagcagga agaccgttgc cgagtagatg aagtagatgg
     1561 gcagttcgtt gtggtaggag cctgcgctgc ccaccaccat ccccccggcg acgaagtaca
     1621 agaagatgtc gtactcgatc tgcgccgggt ccatgaggaa gagcccggac gacccgaaca
     1681 ggatgcccgc cacggcgaac gacacgtagg tgcctgccct ccatatcctc aggtgtgcag
     1741 gggcgaaccg cctgcggtaa aagaagatga tggcgtagcg gctcaatgcg tacaggacgt
     1801 tcacggcaca ccatgtagcc acccgctgcc tgtcgatcac gttccacaag acagccgcga
     1861 tgaccatgga gttcacgatg gtggcagaca tgctcgcggc gaggttcgag atcagcctgt
     1921 cgaacttgtt cagctatacg tagagttccc ggtccgg
//
LOCUS       DAOSCR010000527         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38810_length_1951_cov_3.437236, whole genome shotgun sequence.
ACCESSION   DAOSCR010000527 DAOSCR010000000
VERSION     DAOSCR010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38810_length_1951_cov_3.437236"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLT69_11185"
     CDS             complement(<1..980)
                     /locus_tag="PLT69_11185"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOJ15046.1"
                     /translation="MSIDRIKEIAQAAKRASRTIAMLPRTAKDAALAAMASSLRDAAG
                     FLMEENAKDLEAARSSGLSTAMIDRLTLDEKTISSMAGAMEEIAELPDPVGEITGMKK
                     RPNGLMVGRMRIPLGVVAIIYESRPNVTADAAALCLKSGNAVILRGGSEAFHSNAAIV
                     KVLKGALEAQGIPQEALGFVPVTDRQAILDMLKLEDLIDLVIPRGGEGLIRTVTENSR
                     IPVLKHYKGVCHVFVDRQADLEKAYRICLNAKVQRPGVCNAMETLLVDAPIAGIFLPK
                     MAELYARHGVELRGCPKTMAYLPDIRKATEDDWYAEYLDLVLAVKVVENMG"
     gene            complement(980..>1951)
                     /gene="proB"
                     /locus_tag="PLT69_11190"
     CDS             complement(980..>1951)
                     /gene="proB"
                     /locus_tag="PLT69_11190"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOJ15047.1"
                     /translation="GAIAEGRAIMRVKEADISLSKKQALAAIGQGSLMRAYTDAFRRY
                     GYTAAQILITREDLDNRRRYLNIRNTLSSLFEMGAVPIINENDTVAVEEIQFTDNDML
                     SAMILPLVEAHVLVILTDMEGVYSKDPKIHDDAEIVHEIAELRPKDIRGYGDGGNRLG
                     RGGIASKLEAAYRASMLGVPTVIASAYTPQVISSILEGSQVGTFIHPKKKAQLTQKDH
                     WLGFVSRPKGKVVIDDGAAAMILGRGKSLLPVGVVDVSGSFKAGDPVEIVKRDGTPIA
                     VGLSNFATEEVVKIMGASTSELSMILRYDCDDEVVHRNNMIVRKETF"
BASE COUNT          305 a          645 c          602 g          399 t
ORIGIN      
        1 cccatgttct cgacgacctt caccgcgagg accaggtcga gatactcggc gtaccagtcg
       61 tcctcggtcg ccttccgtat gtcgggcagg tacgccatgg tctttgggca ccccctgagc
      121 tcgacaccgt ggcgcgcgta gagctcagcc atcttgggga ggaagatacc ggcgatgggc
      181 gcgtcgacca ggagggtctc catggcgttg cagaccccgg gacgctggac ctttgcgttc
      241 aggcagatcc ggtaggcctt ctcgagatcc gcctgacggt ccacgaagac gtgacacacg
      301 cccttgtagt gcttcagcac ggggatcctc gagttctcgg tcacggtgcg gatgaggccc
      361 tctccacctc tcggtatcac gaggtcgatg agatcctcga gcttgagcat gtcgagtatg
      421 gcctggcggt ccgtcacggg gacgaacccg agggcctctt ggggaatgcc ctgcgcctcg
      481 agcgcgccct tgagcacctt gacgatcgcg gcgttcgagt ggaacgcctc gctgcccccc
      541 ctgaggatga ccgcgttgcc cgacttgaga cacaaggcgg ccgcgtcagc ggtgacgttg
      601 gggcgcgact cgtaaatgat ggccaccacg ccgagcggta tgcgcatcct cccgaccatg
      661 aggccgttgg gcctcttttt catgcccgtg atctcgccca ccgggtcggg aagctcggct
      721 atctcctcca tggcgccggc catggaggag atggtcttct cgtccaaggt gaggcggtcg
      781 atcatggcag tactcagccc ggatgaacgc gcagcctcga ggtccttcgc gttctcctcc
      841 atgagaaacc cggccgcgtc cctcaacgag gaggccatgg cggcaagcgc ggcgtccttg
      901 gccgtcctcg gcagcatggc gatggtcctg gaggcgcgct ttgccgcctg cgcgatctcc
      961 ttgatccggt caatcgacac tagaacgtct cctttcgaac gatcatattg ttccggtgca
     1021 cgacctcgtc gtcgcagtcg tacctgagga tcatggagag ctcgctcgtg gacgcaccca
     1081 tgatcttcac gacctcctcg gtcgcgaaat tgctcaggcc caccgcgatc ggggtgccgt
     1141 ccctcttcac gatctccacc gggtctcccg ccttgaagct gccggagacg tcgacgacgc
     1201 ccaccggcag gaggctcttg ccccgcccga ggatcatggc cgcggccccg tcgtcgatca
     1261 ccacctttcc cttgggcctg gacacgaaac ccaaccagtg gtccttctgc gtgagctgtg
     1321 ccttcttctt cgggtggatg aaggtgccca cctgggaccc ctcgaggatg gagctgatca
     1381 cctgcggggt gtacgcagag gctatcacgg tgggcacgcc cagcatggat gcgcggtatg
     1441 cggcctcgag cttggacgcg atcccccccc tgccgagcct gttgccgccg tcgccgtacc
     1501 ccctgatgtc cttcggccgc aactcggcta tctcgtgaac gatctctgcg tcgtcgtgga
     1561 tcttcgggtc cttggagtac accccttcca tgtccgtgag gatgacgagg acatgggcct
     1621 cgacgagggg caggatcatc gccgagagca tgtcgttgtc ggtgaactgg atttcctcga
     1681 cggccaccgt gtcgttctcg ttgatgatcg gcacggcccc catctcgaag aggctgctca
     1741 gcgtgttcct gatgttgaga tagcgcctcc tgttgtcgag gtcctccctg gtgatgagga
     1801 tctgggcggc cgtgtaaccg tacctcctga aggcgtccgt gtaggccctc atgaggctcc
     1861 cctgcccgat ggcagccagc gcctgcttct tcgagaggga gatgtccgcc tccttgacac
     1921 gcatgatcgc cctcccctcg gctatggcgc c
//
LOCUS       DAOSCR010000528         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38812_length_1951_cov_3.306435, whole genome shotgun sequence.
ACCESSION   DAOSCR010000528 DAOSCR010000000
VERSION     DAOSCR010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38812_length_1951_cov_3.306435"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLT69_11195"
     CDS             <1..600
                     /locus_tag="PLT69_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15048.1"
                     /translation="ATDVVAAKFLGFDLQDVEHLRLAAAYRLGESDLGRIDIENRHLF
                     EERAQHFSSDLLGRFPEDVRIIRGKTLCCREGCMRNTETVLEVFGRDFGGKGGFTILM
                     GKGIDPAEAASVTGRVHIAGDCAMSEWYPELKARLGARNVTVSPGCNDLAKTIDGLAK
                     QMGIPPYRLSPLSPLKSVALLAEAYLHGTRSNVTNVFRI"
     gene            640..1461
                     /locus_tag="PLT69_11200"
     CDS             640..1461
                     /locus_tag="PLT69_11200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ15049.1"
                     /translation="MMARVAVFFRAAALWALLLALFPSPRASSAHAGETLVFALCYHS
                     FLGSARYAGDVSPAELAGQLDELSSRGFRFVTLSDIDAGRLRGTRNVLVTIDDGNASV
                     YDAYFSVFRPRGIRPLLCIYPGVIGRKSYSLTWERLARLVQDGCEVASHGYYHLGLTS
                     ALFEEDRQAFEHEIFGSRKKLEERLGVRVRSFAYPSGVHSPQARRLLAEAGYSYAFTI
                     LWGPVRLPGDLDGSRLTLPRYMIYRGNWPVVLGAVSKRASSASAQPAASQTPGGR"
     gene            complement(1412..>1951)
                     /locus_tag="PLT69_11205"
     CDS             complement(1412..>1951)
                     /locus_tag="PLT69_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HOJ15050.1"
                     /translation="MRNGRALAWARENGFPVVSDGSNASDLGDYRPGMIASQEDGIWQ
                     PFVELGITKADIRALAQRDGIGPWDRPPSACLCTRFPAGFPLTRELLRRVEAAEAKLV
                     QLGFGDVRVRCFPYDTALIQVAETERLVSMRDEVARFMRGLGFLFVSLDLEGFSSGSL
                     NRIVTGRQAFVMPPAAPKR"
BASE COUNT          317 a          671 c          644 g          319 t
ORIGIN      
        1 gccacggacg tggtggcggc gaagttcctc gggttcgacc tccaggacgt ggagcacctg
       61 aggctcgcgg ccgcgtaccg cctcggcgag tcggacctgg gcaggatcga catcgagaac
      121 cggcatctct tcgaggagag ggcgcagcac ttctcctcgg acctgctggg gcggttcccc
      181 gaggacgtga ggatcatcag gggaaagacc ctgtgctgca gggaggggtg catgcggaac
      241 acggagaccg tgctcgaggt cttcggaagg gacttcggcg gcaagggggg cttcacgatc
      301 ctcatgggca aggggataga cccggcagag gcggcgtccg tgaccgggag ggtgcacatc
      361 gcaggcgact gcgccatgtc cgagtggtac cccgagctca aggcacgcct gggcgcaagg
      421 aacgtcaccg tgtcccccgg ttgcaacgac ctcgcgaaga ccatcgacgg cctcgccaag
      481 cagatgggga tcccgccgta caggctgtcg cccctgagcc cgctcaagtc cgtggcgctg
      541 ctcgccgagg catacctgca cggtacgcgc tcgaacgtga cgaacgtgtt caggatctag
      601 gacacgggcg ccgcttgggc cgttgaggga cgaaacgaca tgatggcacg cgtggcggtg
      661 ttcttccggg cggcagccct ctgggccctt ctcctcgccc ttttcccctc tccccgggca
      721 tcctccgccc acgccgggga gacgctcgtc ttcgcgctct gctaccactc gttcctcggg
      781 tcggcgaggt atgcgggcga cgtctcgccc gccgagctcg ccggccagct ggacgagctc
      841 tcctcgcggg ggttcaggtt cgtgacgctg tccgacatcg atgcagggcg tctcagggga
      901 acccgcaacg tgctcgtcac catcgacgac ggcaacgcga gcgtgtacga cgcgtatttc
      961 tcggtgttca ggccgcgcgg gatcaggccc ctcctctgca tctaccccgg ggtcatcggc
     1021 aggaagtcct acagcctgac gtgggaacgg ctcgcgaggc tcgtgcagga cgggtgcgag
     1081 gtggcgtccc acggttacta ccacctgggt ctgacatccg ccctgttcga ggaggacagg
     1141 caggccttcg aacacgagat cttcgggtcg cggaagaaac tcgaggagcg gctcggcgtg
     1201 agggtccgct cgttcgccta cccgagcggc gttcactcgc cccaagcgcg caggctcctc
     1261 gccgaagccg gctattccta cgcgttcacc atcctctggg ggcccgtccg cctgcccggg
     1321 gacctcgacg gttcgcgcct cacgctcccg aggtacatga tctaccgggg gaactggccc
     1381 gtcgtcctcg gcgcggtctc gaaacgcgct tctagcgctt cggcgcagcc ggcggcatca
     1441 caaacgcctg gcggccggtg acgatcctgt tcaacgaccc gctcgaaaac ccctcgaggt
     1501 ccaggctcac gaacaggaac ccgagccccc tcatgaacct cgccacctcg tccctcatgc
     1561 tgacgagcct ctccgtctcg gcaacctgga tgagggcagt gtcgtaggga aagcacctca
     1621 cgcggacgtc gccgaacccc agctgcacga gcttcgcctc ggccgcctcc accctgcgaa
     1681 ggagctcccg ggtgaggggg aaaccggccg ggaacctcgt gcacaggcaa gcggaaggcg
     1741 gcctgtccca tgggccgatc ccgtctcttt gcgccagggc ccggatatcg gccttcgtta
     1801 tgccgagctc cacgaatggc tgccagatgc cgtcctcttg cgaggcgatc atcccgggcc
     1861 ggtaatcccc gaggtccgag gcgttcgacc cgtcggagac gaccgggaac ccgttctcgc
     1921 gcgcccacgc aagggcgcgg ccgttcctca t
//
LOCUS       DAOSCR010000529         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_38960_length_1946_cov_3.809096, whole genome shotgun sequence.
ACCESSION   DAOSCR010000529 DAOSCR010000000
VERSION     DAOSCR010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38960_length_1946_cov_3.809096"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="PLT69_11210"
     CDS             complement(<1..1549)
                     /locus_tag="PLT69_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase/pyruvate phosphate
                     dikinase"
                     /protein_id="HOJ15051.1"
                     /translation="MTRDDLTDLGERELQAMFRLDFKVFLELMARKIREILLVSSRYD
                     AFVIEEDVTLASRIVTEYSGLNLSQPPRVTRTSSAKRALDLLQERQFDLVITMPNLED
                     MDAFSLGRKIKEKDPALPVYLLAHSLRGLQTSGELVSHEGVDKVFIWSGDAELLLAIV
                     KSTEDAMNAEYDTAHADVCVLILVEDSPLYYSRLLPIFYKEVVRQIQAVLELGVNAEE
                     RLLIMRTRPKILWARTYEEAQELFEKYKQNLLGIISDTRFPRECRMHTDAGVELLSRA
                     RRELPHLPLLLMSSEGENKTKARLIPADFIDKNSPDVIEQIRGFFLRRLGFGEFVFRL
                     PDGKEVGRAAKLRELEERLPTIPEESLMLHIEQKDMANWIMMRSEIPLAKAIRALSCS
                     SFPSASQLRSHVVKLVRSVRRLRQKGVVVQFNRKEFDADLLQFAKIGKGSLGGKARGL
                     AFMSSMLQENMDLLEKYRNAEIRIPRSVVLATDVFEEFVERNDLKRYASEDRPDGEVA
                     RAFLECRL"
     gene            complement(1728..>1946)
                     /locus_tag="PLT69_11215"
     CDS             complement(1728..>1946)
                     /locus_tag="PLT69_11215"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856688.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOJ15052.1"
                     /translation="VATSGLEMTQNSMRLSWTREEVDNRLRMIMKAIHTACVQAAETY
                     GTPHNYVNGANIAGFVKVADAMMAQGIV"
BASE COUNT          319 a          614 c          614 g          399 t
ORIGIN      
        1 ccagcctgca ctcgaggaag gcgcgggcca cctcgccgtc ggggcgatcc tcggaagcgt
       61 accgcttgag atcgttcctc tccacgaatt cctcgaagac gtcggtggcg agcacgacgc
      121 ttcgggggat ccggatctcc gcgttgcggt acttttccag gaggtccatg ttctcctgca
      181 gcatcgagga catgaacgcc agtcctctcg ccttcccgcc gagcgacccc ttcccgatct
      241 tcgcgaactg gaggagatcc gcgtcgaact ccttcctgtt gaactggacg accacgccct
      301 tctgcctgag cctcctcacc gatcggacga gcttgaccac gtggcttcgc aactgggacg
      361 cggacgggaa cgaggagcac gacagtgccc gtatggcctt tgcgagcggt atctcggaac
      421 gcatcatgat ccagttggcc atgtccttct gctcgatgtg cagcatgagc gattcctcgg
      481 ggatcgtcgg gagccgctcc tcgagctccc tgagcttggc cgccctgccc acctctttcc
      541 cgtccggcag cctgaacacg aactcgccga acccgaggcg cctcaggaaa aacccccgga
      601 tctgctcgat gacgtcaggt gagttcttgt ctatgaagtc agccggtatg agacgggcct
      661 tcgtcttgtt ttcgccctcc gagctcatga ggagcagggg gaggtgaggc agctccctgc
      721 gcgccctcga gagcagctcg acgccggcgt ccgtgtgcat cctgcactct ctcgggaagc
      781 gggtgtccga tatgatgccg aggaggttct gcttgtactt ctcgaagagt tcctgtgcct
      841 cttcgtaggt ccgcgcccag aggatcttgg gcctggtcct catgatgagg agacgttcct
      901 ccgcgttgac gccgagctcg agcacggctt gtatctgcct caccacctcc ttgtagaaga
      961 tgggcaggag cctcgagtag tacaggggcg agtcctccac gaggatgagc acgcacacgt
     1021 ccgcgtgcgc cgtgtcgtac tcggcgttca tggcgtcttc cgtgctcttg acgatggcga
     1081 gcagcagctc ggcgtcaccg gaccagatga acaccttgtc gaccccttcg tgggagacga
     1141 gctcgccgga ggtctggagc cccctgaggc tgtgcgcgag caggtacacg ggcagggccg
     1201 ggtctttctc cttgatcttt ctgcccagcg agaaggcatc catgtcctcg aggttgggca
     1261 tggtgatgac gaggtcgaac tgccgctctt ggaggaggtc gagcgccctc ttggccgagg
     1321 acgtcctggt gaccctgggg ggctgggaca ggttgaggcc gctgtactcg gtgacgatac
     1381 gggaagcaag cgtgacgtct tcctcgatga cgaaggcgtc gtagcggctc gatacgagga
     1441 ggatctccct gatcttcctg gccatgagct ccaggaagac cttgaagtcg agcctgaaca
     1501 tcgcctggag ttcccgctcg ccgaggtcgg tgaggtcgtc tcgcgtcaca actggcataa
     1561 taccccgtca aacgggtctt gcaagggccg gcgtacgccc gaacccgccg gtgtcaccag
     1621 gaaaccggcg acctgcggtg gtggtgtgga ggccccgcat gggggcgcag gcacccttgc
     1681 gagtgtctgg gacgccgccc ccatgcgggt ctgtcgactc tgcagcctta cacgatcccc
     1741 tgcgccatca tggcgtccgc caccttgacg aaaccggcga tgttcgcgcc gttcacgtag
     1801 ttgtgcggcg tgccgtacgt ctcggctgcc tgtacgcatg cggtgtggat cgccttcatg
     1861 atcatgcgca gcctgttgtc gacctcttcc ctggtccagg agaggcgcat gctgttctgg
     1921 gtcatctcga gacccgaggt tgccac
//
LOCUS       DAOSCR010000530         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39055_length_1943_cov_2.988877, whole genome shotgun sequence.
ACCESSION   DAOSCR010000530 DAOSCR010000000
VERSION     DAOSCR010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39055_length_1943_cov_2.988877"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PLT69_11220"
     CDS             complement(<1..877)
                     /locus_tag="PLT69_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOJ15053.1"
                     /translation="MKIGFTYDLKDDYLREGFSEEQAAEFDGTDTIEGIENALCSLGH
                     EVDRIGNAQALVRRLARGERWDMVFNIAEGAHGYGREALVPALLDSYGIPYTFSDPLV
                     MAVTLHKGIAKTVVRAAGVPTADFAVVEDPAHVESVGIPFPLFAKPVAEGTGKGIGPA
                     SKVCSMEELRTVCTRLLAQFGQAVLVEEFLPGREFTAGILGTGTRAWVVGVLEVGFRP
                     EKAPERIYSFHTKKHYEELVDYRLADRDAAKACEEVALGAWRSLGCRDGGRVDVRMDE
                     NGRPCFIEVNPLAGLN"
     gene            complement(894..>1943)
                     /locus_tag="PLT69_11225"
     CDS             complement(894..>1943)
                     /locus_tag="PLT69_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOJ15054.1"
                     /translation="RNSITSLEQVRRIVSLTHGEYKGIVRRSSGLPLRITPYYASLLS
                     GLDPDQPIRRAVIPVEDEYLVSDGEEADPLSEEQQSPVPGIVHRYPDRVLFLATGFCS
                     TYCRYCTRSRMVGNHERFKGGFNQWQQAISYIEKTPRIRDVLLSGGDPLTLPDGHLEY
                     LLSSLRRIPHVEIIRIGTKVPVVLPQRITSRLLNILKKYHPLWMSVHFTHPDELTPET
                     RAACNLLADAGIPLGSQTVLLKGINDDVGTLRTLYQGLMTVRVRPYYLYQCDPISGSS
                     HFRTPVEKGLEMIEGLRGHTSGYAVPHYVVDAPGGGGKIPLLPDYFVGRDEGGVLLRN
                     YEGKTYRYPQCPSTR"
BASE COUNT          299 a          629 c          651 g          364 t
ORIGIN      
        1 ggttgagtcc cgcgagcggg ttgacctcga tgaaacaggg tcggccgttt tcgtccatgc
       61 gcacgtccac ccgacccccg tctctgcacc cgagcgacct ccacgccccg agggccacct
      121 cttcgcacgc cttggccgcg tcgcggtcgg ccagcctgta gtcgaccagc tcctcgtagt
      181 gtttcttggt atggaacgag tagatccgct ccggtgcctt ttcgggcctg aagcccacct
      241 ccaagacacc gaccacccag gccctcgtgc ccgttcccag gatgccggcg gtgaactctc
      301 tccccgggag gaactcctcc acgagcacgg cctgcccgaa ctgggccaag agcctcgtgc
      361 agacggtgcg cagctcctcc atggagcaga ccttggaggc ggggccgatc cccttgcccg
      421 tgccctcggc taccggtttc gcaaagaggg ggaaggggat gcccacgctc tcgacgtggg
      481 ccgggtcttc caccaccgcg aagtccgcgg tcgggacccc tgcagccctc accacggtct
      541 tcgcgatgcc cttgtggagc gtcactgcca tgacgagggg gtcggaaaag gtgtacggaa
      601 tcccgtacga gtcgaggagg gcggggacga gggcttccct cccgtagccg tgggcgccct
      661 ccgcaatgtt gaagaccatg tcccagcgct cacccctggc gagccttcga accaaggcct
      721 gtgcgttgcc gatgcggtca acctcgtgcc ccagggagca gagggcgttc tcgataccct
      781 cgatggtgtc cgtcccgtcg aactcggcgg cctgctcttc cgagaagccc tcccgcaggt
      841 agtcgtcctt gaggtcgtat gtgaagccga tcttcatgcg ggcagttgat ggttcaccgc
      901 gtgctcgggc attgggggta cctgtaggtc ttgccctcgt agttcctgag cagcacaccg
      961 ccctcgtcgc ggccgacgaa gtaatccggc aggaggggta tcttcccccc cccgcccggg
     1021 gcgtccacca cgtagtgagg cacggcgtat cccgacgtgt gccccctgag gccttcgatc
     1081 atctcgaggc ccttctcgac tggggtgcgg aagtgggagg atcccgagat gggatcgcac
     1141 tggtagaggt agtagggccg gacccgcacg gtcatgagac cctggtagag cgtgcggagc
     1201 gtgcccacgt cgtcgttgat gcccttgagg aggaccgtct ggctgcccag ggggatgccc
     1261 gcgtccgcca ggaggttgca tgcggcgcgc gtctccgggg tgagctcgtc cgggtgagtg
     1321 aagtgtacgc tcatccacag cgggtggtac ttcttcagga tgttcagcag gcgcgaggtg
     1381 atgcgctgcg ggagcacgac cggcaccttg gtgccgatgc ggatgatttc cacgtggggt
     1441 atccgcctga ggctcgacag gaggtattcc aggtgaccgt caggcagggt gagcgggtct
     1501 ccgcccgaca ggaggacgtc ccgtatccgg ggtgtcttct cgatgtacga gatggcctgc
     1561 tgccactggt tgaagccgcc cttgaagcgt tcgtggttcc ccaccatgcg agagcgcgtg
     1621 cagtacctgc agtaggtcga gcagaagccc gtggcgagga acagcacccg gtcggggtat
     1681 cggtggacga tgccgggcac cgggctctgt tgctcctcgg acagggggtc cgcctcctct
     1741 ccgtcggata cgaggtactc gtcttccacc gggatcaccg ccctgcgaat cggctggtcg
     1801 gggtcgagcc cgctcaggag gctcgcgtag tagggcgtta tcctcagcgg gagacccgag
     1861 gagcgcctca cgatgccctt gtactcgccg tgggtgaggc tcacgatgcg cctgacctgc
     1921 tccaggctcg tgatgctgtt tcg
//
LOCUS       DAOSCR010000531         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39107_length_1941_cov_3.493107, whole genome shotgun sequence.
ACCESSION   DAOSCR010000531 DAOSCR010000000
VERSION     DAOSCR010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39107_length_1941_cov_3.493107"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLT69_11230"
     CDS             complement(<1..952)
                     /locus_tag="PLT69_11230"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HOJ15055.1"
                     /translation="MIRLLCVGASLAGLLCLALAGLLPWVVFAALAGVHLMVLRYFFD
                     REVISAPVSLALVAVVSSWNAWRIFEHGADDALWAVRDLVVAIAVVRLLSRKTGREIL
                     QILGIALSEAIFATILTQSATFLFGMCCMALILPMALDAVDAEEFRAPVKSGAAGARH
                     WARVFAGIVCSSCVLFYLLPRPASPIVRHSLVHDRRHTFGEDVDLGSQAAPPGDTAVL
                     MRIVWDSPDRPKSFYLAGARLEVMTPDGFFHAPDGDTAPGASPCEAKPTDRLTVYQTG
                     IDARYVFHPFTVCRVRPAAVRSVGPNLFWQYDVPLKYDLWV"
     gene            complement(963..>1941)
                     /locus_tag="PLT69_11235"
     CDS             complement(963..>1941)
                     /locus_tag="PLT69_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15056.1"
                     /translation="RRPGARGRPHEREPGRHPVGFGDLKRILKPPRGFRLTKAGKVFF
                     AFLVCLIFVSLSTGNNLLYLVLAAMLGFMVVSGVQSELNLRHLEVRRLLPSEIHAGVQ
                     ARVGYAVRNPRHDSTRLVLEDLSRVRIPMLNRGEESVFQVSVIFAQRGLVPLGDVRIA
                     TTYPYGLFEKSISFPARGEAVVFPRPLSVGFALSKGSLDTGTGATRETVSHVRPYTPG
                     DPLSNVAWKKRQPGLVSRVFEGGSGSSGVVVLTPGPDVETKLSQAAYVIDELHRCAKP
                     FGLLVGTTFSGMGTARSHRIEVLTRLALVERIPAPSYEVIPGDADIVIV"
BASE COUNT          372 a          642 c          637 g          290 t
ORIGIN      
        1 tcacccagag atcgtacttg agcggcacgt cgtattgcca gaacaggttc ggcccgactg
       61 agcggaccgc cgccggcctc accctgcaca cggtgaaggg gtgaaagacg tacctggcgt
      121 cgatcccggt ctggtacacg gtaagcctgt ccgtgggttt cgcctcacaa ggcgaggcac
      181 ctggagctgt gtcaccgtca ggggcgtgaa agaaaccgtc gggcgtcatg acctcgagcc
      241 tcgcccctgc caggtaaaag ctcttgggcc tgtcggggga atcccagacg atgcgcatca
      301 ggacagccgt atcgcctggg ggcgcggcct gcgagccgag gtccacgtcc tcgccgaagg
      361 tgtggcgcct gtcatgcacg agcgagtggc ggacgatggg ggacgccggc ctcggcagga
      421 ggtagaaaag gacacaggag gagcacacga tacccgcgaa cacgcgcgcc cagtgtcgtg
      481 cacccgccgc cccgctcttt accggggccc tgaattcctc cgcgtcaacg gcgtcgagcg
      541 ccatggggag gatcagggcc atgcagcaca tgccgaaaag gaacgtggcg ctctgcgtca
      601 ggatggtggc aaagatcgcc tcggacagcg cgatgccgag gatctggagg atctcgcgcc
      661 cggtcttcct cgacagcagc ctcacgacgg cgatggccac cacgaggtcc ctcaccgccc
      721 acagggcgtc gtccgccccg tgctcgaaga tcctccacgc gttccaactc gagaccacgg
      781 cgacgagggc gagcgaaacc ggtgcggaga tcacctccct gtcgaaaaaa taccggagaa
      841 ccataaggtg cacgcctgcg agcgccgcga acacgaccca cgggagaagc cctgcgagcg
      901 cgaggcatag caggcccgcg agcgaggcgc ccacacagag cagcctgatc atgaacggcg
      961 tctcatacga tgacgatgtc cgcatcaccg ggaatgacct cgtacgaagg tgctggaatc
     1021 ctctccacga gggcgagcct cgtgagcacc tcgatcctgt gtgacctggc cgtgcccata
     1081 ccgctgaacg tggtccccac gaggaggccg aacggtttcg cacaccggtg gagctcgtcg
     1141 atcacgtacg cggcctgcga gagtttcgtc tcgacgtccg gcccgggtgt caggaccacc
     1201 accccggacg acccggagcc gccctcgaac acccgcgaga ccagcccggg ctgcctcttc
     1261 ttccaggcca cgttggacag ggggtcgccg ggcgtgtacg gcctcacgtg ggacacggtc
     1321 tcgcgggtcg cgcccgtccc cgtgtcgagg gaacccttcg acagggcgaa cccgacggac
     1381 aggggccggg ggaacaccac ggcctcgccc cttgccggga aggagatgga cttctcgaag
     1441 agcccgtagg gataggtggt cgcgatcctc acgtcaccga ggggtaccaa gcccctctgt
     1501 gcgaaaatca cggagacctg gaacaccgac tcttctcccc tgttcagcat gggtatacgg
     1561 acacgggaga ggtcctcgag cacgaggcgg gtggagtcgt gcctggggtt cctgaccgcg
     1621 tagcccaccc ttgcctgcac cccggcgtgt atctctgacg gcaggaggcg tctgacctcg
     1681 aggtggcgca ggttgagctc cgactgcacc ccggagacca ccatgaagcc gagcatggct
     1741 gccagcacga gatagaggag gttgttgccg gttgagagcg agacgaagat caggcagacg
     1801 aggaaggcga agaagacctt gcccgccttg gtgagcctga accccctcgg cggcttcagg
     1861 attctcttga gatccccgaa ccccactgga tgtctcccgg gctcccgttc gtgcggtcgg
     1921 cccctcgcgc ccggcctccg a
//
LOCUS       DAOSCR010000532         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39152_length_1940_cov_3.171353, whole genome shotgun sequence.
ACCESSION   DAOSCR010000532 DAOSCR010000000
VERSION     DAOSCR010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39152_length_1940_cov_3.171353"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLT69_11240"
     CDS             complement(<1..237)
                     /locus_tag="PLT69_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOJ15057.1"
                     /translation="MKKIEAIIKPFRLDEVKDALQQVGASGMTITEVRGHGRQKGHRE
                     IYRGAEYTVDLIPKVKIELVVADNAVEGVVRTIME"
     gene            complement(275..1480)
                     /locus_tag="PLT69_11245"
     CDS             complement(275..1480)
                     /locus_tag="PLT69_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOJ15058.1"
                     /translation="MVDSGHTAWMLVSTALVMLMTPGLALFYGGMVRSENVLATIMQS
                     FSCLGLVTVLWVLFGYSLAFGPDVMGVVGSWQWAGLSGVGMDPGPYSDKIPHLLFCSF
                     QLMFAAITPALITGAFAERLTFRSFVVFTLAWTTLVYLPVCHWVWGGGWMGKLGALDF
                     AGGTVIHVNSGAAALAAALITGKRRGYRTEPMYPHNLPLTMLGAGLLWFGWFGFNAGS
                     ALSAGSQASLAFLSTQVATAAAALAWIGCERVIRKKPTTLGFASGAVAGLVAITPGAG
                     YIDPVSALAVGAVAGAVCYFAVLMKERLGYDDSLDVVGIHGAGGLWGAVATGLFASYG
                     GTVLFHGNPHQLVVQVTGALATATYSFLATSLILLAMKGLMPLRVEEEHEVEGLDRSE
                     HGESAYWIR"
     gene            1711..>1940
                     /locus_tag="PLT69_11250"
     CDS             1711..>1940
                     /locus_tag="PLT69_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ15059.1"
                     /translation="MRKFSVSDLQVLHSIAKVVASTMDLRTQLATVLNLLSTETGMNR
                     GMISILERDTKAAVLDVAHGVDIEGLDITYLPG"
BASE COUNT          392 a          639 c          572 g          337 t
ORIGIN      
        1 ttccatgatg gttctcacca ccccctcgac cgcgttgtcc gccacgacga gctctatctt
       61 caccttgggg atgaggtcca cggtgtactc ggcaccccta tagatctccc tgtgcccttt
      121 ctggcggccg tgtcccctga cctcggtgat ggtcatcccc gacgccccga cctgctggag
      181 agcgtccttc acctcgtcga gcctgaatgg cttgatgatc gcctctatct tcttcatgtc
      241 gtccctccgt tgtgatccaa gcggtatgtc acccctacct gatccagtat gcagactcgc
      301 cgtgctcgct cctgtcgagc ccttccactt cgtgttcctc ctccaccctc aagggcatca
      361 ggcccttcat ggcgagcagg atgagggagg tcgccaagaa cgagtacgtc gccgtcgcga
      421 gtgcgccggt gacctgcacc acgagctggt gcgggttgcc atggaacaac accgtgcccc
      481 cgtaggacgc gaagagccct gtggcgacgg ctccccagag accgcctgcg ccgtggatcc
      541 ccaccacgtc gagggaatcg tcgtacccga gtctctcttt catgaggacc gcgaagtaac
      601 agacggcgcc cgctacggcc cccacagcca gtgcgctcac gggatcgata taccctgcac
      661 ccggggttat ggcgaccagg cccgccaccg cgcctgaggc gaaacccagg gtcgtgggct
      721 tctttcggat cacgcgctcg cacccgatcc aggcgagtgc ggcagcggcc gtggcgacct
      781 gagtcgagag gaacgcgagg ctcgcctggg acccggcgga aagcgcgctg ccagcgttga
      841 acccgaacca cccgaaccag aggagccctg cccccagcat ggtcagtggc aggttgtgtg
      901 gatacatggg ctcggtcctg tagccccttc tcttgcctgt gatgagcgca gcggcaaggg
      961 ccgcggcgcc cgagttcaca tggatcaccg tcccgcccgc gaagtcgagc gccccgagct
     1021 tgcccatcca gccgcctccc cagacccagt ggcatacggg aaggtacacg agcgtcgtcc
     1081 acgcgagcgt gaagaccacg aacgacctga acgtgagcct ctctgcaaac gctcccgtga
     1141 tgagcgccgg cgtgatggcg gcgaacatca gctggaacga gcagaagagg aggtggggga
     1201 tcttgtccga gtacggaccc ggatccatgc caaccccgga caggccggcc cactgccaag
     1261 aacccacgac gcccatgaca tcggggccga aggcgaggct gtagccgaag aggacccaga
     1321 gcaccgtgac cagaccaagg cacgagaagc tctgcatgat ggtcgcgagc acgttctcgg
     1381 acctgaccat gcctccgtaa aagagtgcca gccccggggt catgagcatc accagcgccg
     1441 tcgatacgag catccacgcg gtatgtcctg aatctaccat ggagtcctcc ctgtgtgtcg
     1501 agttgctggc cgttagtcaa gatccatgcc gtttccggaa aacatagaca tatcagtatg
     1561 atagaaggca gaaggggatc tccgggtgtc tcaaagggca caaaaatgct tcaaatcgga
     1621 aaagtcggca ccaaaatgag taacgtgtgg acaagacgcc ttgggtgttg tattgcacct
     1681 ctgacaaggt accggagagg aaaggcgccc atgagaaagt tcagtgtttc ggacctgcag
     1741 gtactccact cgatcgccaa ggtcgtggcc tcgaccatgg acttgaggac ccagctcgcc
     1801 acggtcctca acctcctgag caccgagacc ggcatgaacc ggggcatgat ctccatactc
     1861 gagcgcgaca ccaaggccgc ggtcctcgac gtggcgcacg gcgtggacat cgagggcctc
     1921 gacatcacct atctcccggg
//
LOCUS       DAOSCR010000533         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39251_length_1937_cov_3.429862, whole genome shotgun sequence.
ACCESSION   DAOSCR010000533 DAOSCR010000000
VERSION     DAOSCR010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39251_length_1937_cov_3.429862"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLT69_11255"
     CDS             <1..654
                     /locus_tag="PLT69_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ15060.1"
                     /translation="CVGCISLQVKGRPPSPQKRITFVPTPEEIAEVALRHIATADSPV
                     VSFGQGCEGEPLIQAETICRAIGLVRSKTTAGTINLNTNASMPGMVARLADAGLDSIR
                     ISMNSARRETYEKYFRPSYPFDALSESAREMKKRGRFVSINLFVFPGLTDAPKEAQAL
                     RRFVEDNEVDMIQWRNLNLDPDVYLEMLGQNLPAGVGIRRLMDEIPCRRGYFNPYLG"
     gene            complement(881..>1937)
                     /locus_tag="PLT69_11260"
     CDS             complement(881..>1937)
                     /locus_tag="PLT69_11260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ15061.1"
                     /translation="HADLVLDEFDTRIYEKTMHTVEPREGRRFSVVIFSDVTSSLRQR
                     EELERLLIESAETRDLMEEQAARLAITLAEMDEKNEIIRQQNQRMIEELEMAARLQKS
                     LLPDTFENLNGVSFSCKYIPSIHIGGDLYDVVDLGGGQSGFIIADVSGHGVAAALVSA
                     MFKMSFHALASTVASPTILMHMLNKELRPVLEEDYITAFFVITDRYNKGITYTNAGHP
                     TPLLFRRATGMIEELDTDGFFLGSFDDGGYEEKTVSDIEKGDALLMYTDCILEAENPE
                     GEQYGKERLKACFARCMESLRGNEVIEAIEADVRAFAAKDNLDDDLTVLLLEFWEEVG
                     PGEEMPADGDTGGFVEF"
BASE COUNT          339 a          621 c          620 g          357 t
ORIGIN      
        1 tgtgtaggct gcatatcgct ccaggtcaag ggcaggccgc cctcccccca gaagcggata
       61 acgttcgtgc cgaccccgga ggagatcgcc gaggtggcgc tcaggcacat agcaacggcc
      121 gactctccgg tggtgagctt cggccagggc tgcgagggag agccgctgat ccaggccgag
      181 accatctgca gggccatagg gctcgtcagg tcgaagacca cggcaggcac catcaacctc
      241 aacacgaacg cgagcatgcc cggcatggtg gcgaggcttg ccgacgcagg gctggacagc
      301 atccgcatca gcatgaactc ggcgcgcagg gaaacctacg agaagtactt caggccttcc
      361 taccccttcg atgcgctctc ggagagcgcg agggagatga agaagagggg gcggttcgtc
      421 tccatcaacc tcttcgtgtt cccggggctc accgacgcac ccaaggaggc ccaggccctc
      481 aggcgtttcg tcgaggacaa cgaggtggac atgatccagt ggcggaacct caacctcgac
      541 ccggacgtat acctggagat gctgggccag aacctgcctg caggggtagg gatacggcgc
      601 ctcatggacg agatcccctg caggcgcggt tacttcaacc cctacctggg gtaggagggg
      661 ccgccctcga cgtggaggac ttgggcagtg tctcgagtga gaccgccgcg tgggagaaac
      721 aggcgcccga gcccggcctc agcgccatga gacggcggcg cattaccggc agggtttcag
      781 ggcctcggtg cgcggtcgcc acgcctgtcc caacatgcaa cggtcgggcc tcgacaggtg
      841 aacagccttg ccgggcctcc tccatgtcgg ccgcatcctg tcagaactcc acgaacccgc
      901 cggtatcccc gtccgcgggc atctcctcgc ccgggcctac ctcttcccag aactcgagga
      961 gcaacacggt gaggtcgtcg tcgaggttgt ctttggcggc gaaggcgcgc acatcggcct
     1021 cgatggcctc gatgacctcg tttccccgca gggactccat gcagcgcgcg aagcacgcct
     1081 tgagcctctc cttgccgtac tgctctccct cggggttctc cgcctcgagg atgcagtcgg
     1141 tatacatgag cagcgcatcg cccttctcga tgtcgctcac cgtcttctcc tcgtacccgc
     1201 cgtcgtcgaa agacccgagg aagaagccgt ccgtgtcgag ttcctcgatc attcccgtcg
     1261 cgcgcctgaa gaggagaggg gtggggtgcc ccgcgttcgt gtacgtgatg cccttgttgt
     1321 agcggtcggt tatcacgaag aaggcggtga tgtagtcctc ctcgaggacc ggcctgagct
     1381 ccttgttgag catgtgcatg aggatcgtgg ggctcgccac ggtggatgcg agtgcgtgga
     1441 agctcatctt gaacatggcc gagacgaggg ccgccgccac cccgtgcccg gacacgtcgg
     1501 cgatgatgaa accagactgt ccgccgccca ggtcaaccac gtcgtagagg tctccaccta
     1561 tgtggatgga cgggatgtac ttgcaggaga agctcacgcc gttgaggttc tcgaaggtgt
     1621 cggggagcag gctcttctgg agcctcgcgg ccatctcgag ctcctcgatc atccgctggt
     1681 tctgctggcg aatgatctcg ttcttctcgt ccatctccgc cagcgtgatc gcgagcctcg
     1741 ccgcctgttc ctccatgagg tcccgggtct ccgcgctctc gatgaggagc ctctcgagct
     1801 cctccctctg ccgtagggac gaggtcacgt cggagaagat cacgaccgag aatctcctgc
     1861 cctccctcgg ttcgacggta tgcatggtct tctcgtagat gcgggtgtcg aactcgtcga
     1921 ggacgaggtc ggcgtgg
//
LOCUS       DAOSCR010000534         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39286_length_1936_cov_2.951090, whole genome shotgun sequence.
ACCESSION   DAOSCR010000534 DAOSCR010000000
VERSION     DAOSCR010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39286_length_1936_cov_2.951090"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1936
                     /locus_tag="PLT69_11265"
     CDS             <1..>1936
                     /locus_tag="PLT69_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ15062.1"
                     /translation="DAGLTRLKQDTSAQHRLFSSGGLDRLRAVSDRLKTFVEHEEDAL
                     ERASGRLNVRDTEVMAERLVRLKDVDWALREDLIAVHDKVRALAGPLMYEDPAYYARF
                     QKINACLNALDRLEVRGRDSAGIQVQVCYPDLESVALLRHRLTQDALFEEFVQRSTPM
                     ELKNGSISLHGPVISFTYKTAQITGELGENTRKLRAFIGSDGVLKAALSIDCVTETYL
                     AHTRWASVGAINETNCHPVNNHCLDAPVEELGGLPSRCRDYPRYGKGAWTVSAVLNGD
                     IDNFQGIRNSLEDLGRVLSTEVTTDTKAIPLQVERFLQEGDTLAEAFRKAVNSFEGSH
                     AVALQSNIEPGKVFLAQRGSGQTIYVGLLENGYMFASEIYGLVEVTSRFIKMDGETPR
                     VPGDTSTQGQIFILSDGASGGVDGIEAMYYDGHPVTLSEKDIQTAQITTRDIDRGEHQ
                     HFLIKEILDAPSSIRKTLRGKYRRVSPGKVVFNLDETVVDPHTREMLVRNEIRKIFVV
                     GQGTAAVAASAVAEALGVYLRGAAVTVGSRKATDLSGFMLDDDMYDTLVIAITQSGTT
                     TDTNRAVTLARQRGARVMAIVNRRQSDITTKVHGVFYTSDGRDIEMSVASTKAFYSQI
                     VAGYVLALFFAQQLGTLEDGA"
BASE COUNT          386 a          598 c          651 g          301 t
ORIGIN      
        1 ggacgccggg cttacccgcc tgaagcagga cacctcggct caacaccggc tgttctcgtc
       61 gggtgggctc gaccgcttga gggcggtgtc ggacaggctc aagacgttcg tggagcacga
      121 ggaagacgcc ctcgagaggg catcggggag gctcaacgtc agggacaccg aggtcatggc
      181 cgagcgtctc gtgagactca aggatgtcga ctgggcgctc agggaggatc tcatcgcggt
      241 ccacgacaag gttcgtgcgc tggcgggccc gctgatgtac gaggatcccg cctactacgc
      301 gaggttccag aagatcaacg cgtgcctgaa cgcactggac cggctcgagg tgagaggacg
      361 ggactctgcg gggatccagg tccaggtgtg ctacccggac ctggagtccg tggccctgct
      421 tcgtcaccgg ttgacccagg acgccttgtt cgaggagttc gttcagaggt caacccccat
      481 ggaactcaag aacggctcca tcagccttca cggcccggtg atatccttca cgtacaagac
      541 cgcgcagatc acgggcgagc tcggcgagaa cacgaggaag ctcagggcgt tcatagggtc
      601 cgacggcgtc ctgaaggcgg ccctgtccat agactgcgtg actgagacgt atctcgcgca
      661 cacgcggtgg gcctcggtcg gcgccatcaa cgagaccaac tgccaccccg tcaacaacca
      721 ctgccttgac gcgcccgtcg aggaactcgg ggggttgcct tcgcgatgca gggattatcc
      781 tcgctacggg aagggtgcgt ggaccgtgag cgcggtcttg aacggcgaca tcgacaattt
      841 ccagggcata aggaacagcc tcgaggacct ggggagggtg ctgagcaccg aggtcaccac
      901 ggacaccaag gccatacccc tccaggtgga gcgctttctc caggagggtg acaccctcgc
      961 cgaggcgttc aggaaggcgg tgaactcctt cgagggttcc cacgccgtag cgctccagag
     1021 caacatcgag ccgggaaagg tgttcctggc gcagaggggg agcggccaga ccatctacgt
     1081 gggcctgctc gaaaacggct acatgttcgc ctcggagatc tacggcctcg tggaggtcac
     1141 ctccaggttc atcaagatgg acggggagac cccgcgggtg cccggggaca cctcgaccca
     1201 ggggcagatc ttcatcctgt ccgacggcgc gagcgggggg gtcgacggca tcgaggccat
     1261 gtactatgac gggcaccccg tcaccctgtc ggagaaggac atccagaccg cccagataac
     1321 cacacgggac atcgacaggg gggagcacca gcacttcctc ataaaggaga tactcgacgc
     1381 gccctcctcc atccgcaaga cgctcagggg caagtaccgc agggtctcgc cggggaaggt
     1441 ggtgttcaac ctcgacgaga cggtcgtgga tccgcacacc agggaaatgc tcgtgagaaa
     1501 cgagatacgc aagatattcg tcgtgggcca gggaaccgcg gcggtcgccg cgagcgcggt
     1561 cgcggaggcc cttggtgtgt acctgcgggg tgcggccgta acggtggggt cgcgcaaggc
     1621 gaccgacctg agcgggttca tgctcgacga cgacatgtac gacacgctcg tgatcgccat
     1681 cacgcagtcg ggcacgacca cggacaccaa ccgtgcggtg accctggcgc gccagagggg
     1741 ggccagggtc atggccatcg tgaaccgcag gcagtcggac atcacgacca aggtccacgg
     1801 cgtgttctac acgagcgacg gcagggacat cgagatgtcc gtcgcgtcga ccaaggcctt
     1861 ctactcgcag atcgtcgccg ggtacgtgct cgccctgttc ttcgcccagc agctcggcac
     1921 cctcgaagac ggggcg
//
LOCUS       DAOSCR010000535         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39291_length_1936_cov_2.798511, whole genome shotgun sequence.
ACCESSION   DAOSCR010000535 DAOSCR010000000
VERSION     DAOSCR010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39291_length_1936_cov_2.798511"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLT69_11270"
     CDS             <1..1035
                     /locus_tag="PLT69_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15063.1"
                     /translation="ECDVFSFFESGPVVTVSVVRIAKGAIEDSRALSLRKAAQDDVVS
                     MAVLQFYLAGNTVPPLVYLDDDAGSRHKTLGRALERLKGARVRVAVPRRGRPLEWLEI
                     ARQNARGHSLDKGPSALEEIAEAFHLKVLPFRMECLDVSTLGGTYSTASCVAFLGGEP
                     AKNLYRRYRIRDDSAKDDYSMLREVLARRLAGDEARPDLLVIDGGKAQLNVFLRVARE
                     LGIESFPVVAMAKARGATQDRFYLPGRKDPVRLGPKSKAMLTLQRLRDEAHRFAVGYH
                     RRLRSKGNITSLFEDIPGIGPVKARALLQLASRGRDISAIGRSDLEGLGCLNRKDIEH
                     ILDHLNRLRP"
     gene            1211..>1936
                     /locus_tag="PLT69_11275"
     CDS             1211..>1936
                     /locus_tag="PLT69_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15064.1"
                     /translation="MARDLSRIPFTFAGEYGPLLRPLMRRLFSRISTPEIFKASMKNL
                     STKGHVVYALASSSTVDALLLNMRLRNEGLPAPMLVFSKTFFLLQSVFCALRDIAHRV
                     KGETPFSHDSYVEFMKDRLRASLVFLDESRPASGADVILELLKLQRRIDDPVFVVPVR
                     IVYQRAPLKLKDTSDEEQLRITDARKLLVLLKGRERGFVEHGEPLNLKEMLKKDVSGR
                     KFVEELAGEITSELRHTLAVLGSN"
BASE COUNT          356 a          629 c          613 g          338 t
ORIGIN      
        1 gagtgcgacg tcttttcctt cttcgagtcc ggccccgtcg tcaccgtttc cgtggtcagg
       61 atcgcaaagg gcgccatcga ggacagcagg gctctttccc tgaggaaggc cgcccaggat
      121 gacgtggtgt ccatggcggt tctccagttc tatctcgcag ggaacacggt gccgccgctt
      181 gtctacctgg acgacgacgc cgggtcccgg cacaagaccc tgggcagggc cctcgaacgt
      241 ctcaagggcg cgcgggtgag ggtggcggtc cccaggaggg ggagacccct ggagtggctc
      301 gagatcgcga ggcagaacgc gcggggccac agtctggaca aggggccctc ggcgctggag
      361 gagatcgcgg aggccttcca cctgaaggtc ttgcccttca ggatggagtg tctcgacgtc
      421 tccaccctcg gggggacgta ctccacggcg tcatgcgtcg cgttcctcgg cggagaacca
      481 gcgaagaacc tctacaggag gtaccgcata cgcgacgact cggccaagga cgattactcg
      541 atgttgaggg aggtgctcgc gaggcgtctc gcgggcgacg aggccaggcc ggacctcctc
      601 gtcatcgacg gcggcaaggc ccaactcaac gtctttctcc gtgtggcccg ggagctcggc
      661 atcgagtcgt tccctgtggt cgccatggcc aaggcgcggg gggcgaccca agacaggttc
      721 tacctgcccg ggaggaagga cccggtgcgc ctcgggccga agagcaaggc catgctcacc
      781 ctgcagaggc tcagggacga ggctcaccgg ttcgccgtgg gataccacag gcgcctgagg
      841 tcgaagggga acatcacctc cctgttcgag gacatacccg gtatcgggcc ggtcaaggct
      901 cgggccctgc tccagctggc ctcgcgcggc cgggatatct cggcgatcgg gaggtcggat
      961 ctcgagggtc tcggatgcct gaaccgcaag gacatcgaac acatcctcga tcacctgaac
     1021 cgcctgagac cctgatgatc cccgatgatc ccccgtgcgc acacaggcgg cctcgctgtc
     1081 cgtgcgtatc gtcttgtatg atggccgccg ccaaaagagg gcccatacgc ggatacccga
     1141 ggacggcggg gtacatgcac gggcaactgg catctttcca ttccgtcttg aatgatctat
     1201 gatcccggcc atggctcggg acctgtcgcg catccccttc acctttgcag gcgagtacgg
     1261 ccccctgctg aggccgctga tgcgaaggct cttttcccgc atctcgaccc cggagatctt
     1321 caaggcctcg atgaagaacc tctccacgaa agggcacgtc gtctatgcac tcgcatcgtc
     1381 gagcaccgtc gacgccctgc tcctcaacat gagattgaga aacgaggggc tgcccgcccc
     1441 catgctcgtg ttctcgaaga cctttttcct gctccagagc gtgttctgcg ccctcaggga
     1501 catcgcccac cgggtcaagg gggagacccc cttttcccat gattcttacg tggagttcat
     1561 gaaagaccgg ctcagggcat ccctcgtgtt cctggacgaa tcacgcccgg cgagcggggc
     1621 ggacgtcatc ctcgagctgc tcaagctcca gcgcaggatc gatgaccccg tgttcgtcgt
     1681 cccggtccgc atcgtctacc agcgcgcccc gctcaagctc aaggacacca gcgacgagga
     1741 acagctgcgc ataacggacg cgaggaaact cctcgtcctc ctgaagggcc gggagcgcgg
     1801 tttcgtggaa catggggagc ccctgaacct caaggagatg ctcaagaagg acgtctccgg
     1861 ccgaaagttc gtagaggaac tggccggcga gatcacctcc gaactcaggc acaccctggc
     1921 tgtcctgggc agcaac
//
LOCUS       DAOSCR010000536         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39332_length_1935_cov_2.477660, whole genome shotgun sequence.
ACCESSION   DAOSCR010000536 DAOSCR010000000
VERSION     DAOSCR010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39332_length_1935_cov_2.477660"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLT69_11280"
     CDS             <1..639
                     /locus_tag="PLT69_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15065.1"
                     /translation="AFSSSKSYFGPANIQSNGERIAAALLGNTNPNLDCGTPVPTPTG
                     LALALTTVYVDMSLADILNGFQAMAADFIVQSILNWLGNKAGELIEPYASYLLGRFAP
                     QVLSKQAAKALLRSRGGVPGNMINEAARQLVQSQRVSAAAYFSALPARSGNLANAVTG
                     FFMGGPMGMDASTFGLPTLLGRQAEAVTNYLSNPGVDEHGGSTGGAGQTGAY"
     gene            649..1806
                     /locus_tag="PLT69_11285"
     CDS             649..1806
                     /locus_tag="PLT69_11285"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HOJ15066.1"
                     /translation="MPVLTSPAPCAMQLSGRDPQGGFILSVVLKVAYAVGKDGSLEPM
                     PEQEPLNTRPVADGSVKGLLVHDIDIYPYKLSTDVVIKGHAYGHGRRRFIAGVSVAGR
                     EKRVLVVGDRSCTLDPAGKIVFSEPEPVDKVPLSYTHAYGGRDHAAERTRGNPFLALH
                     GAVDPGECDLDMASPFLYPRNPCGTGYLIEKDGRAVEALRLPNLEDPQDPLTPERLCV
                     QDHLRWPLMPLPQAMDWYDYGWFPRIACAGIVPDHDEAAAVFAEVDRGLVPAWVVKDR
                     TVDAHGAFLLASGASPGLSVPYLVGGEEILLGNMHPGRETMRIRLPKKAPRLFADARN
                     GRLNRTDVVMHTVVIEPDRDRVCIVWRGSAPALRPYLPQELAGMPFMAEWP"
BASE COUNT          336 a          634 c          667 g          298 t
ORIGIN      
        1 gccttcagct cgtcgaagtc gtacttcggg cccgcgaaca tccagtcgaa cggggagcgc
       61 atcgccgcgg ccctcctcgg gaacacgaac ccgaacctcg actgcgggac ccccgtgccc
      121 acgcccaccg ggctcgcact cgcgctcacc acggtgtacg tggacatgag cctcgcggac
      181 atcctcaacg gctttcaagc catggcggcg gacttcatcg tgcagtccat cctcaactgg
      241 cttgggaaca aggcgggaga gttgatagag ccttacgcga gctacctcct cggcaggttc
      301 gcaccccagg tcctctccaa gcaggcggcg aaggccctgc tcaggagcag ggggggtgtg
      361 ccggggaaca tgataaacga ggcggcccgg cagctggtgc agtcgcagcg tgtatccgcg
      421 gcggcctact tcagcgcgtt gccagcgaga tcgggcaacc tggccaacgc cgtcacgggt
      481 ttcttcatgg gcgggcccat gggcatggac gcttccacgt tcggtctgcc gacgctcttg
      541 ggaaggcagg ccgaggccgt cacgaactac ctgagcaacc ccggcgtcga cgagcacggc
      601 ggctcgactg gcggtgccgg gcagaccggg gcgtactagg gagggaccgt gcccgtcctc
      661 acatcccccg cgccgtgcgc gatgcagctg tcgggaaggg acccccaggg agggttcatc
      721 ttgtcggtgg tgctcaaggt cgcctatgcc gtcgggaagg acgggtccct cgagccgatg
      781 cccgagcagg agccgctcaa cacgagaccc gttgccgacg ggtccgtcaa gggcctgctc
      841 gtccacgaca tcgacatcta cccctacaag ctctccacgg acgtggtgat caaggggcac
      901 gcctacggcc atgggaggag gaggttcatc gccggggtga gcgtcgccgg cagggagaag
      961 cgggtcctcg tggtgggtga caggagctgc acgctggacc ctgccgggaa gatcgtgttt
     1021 tccgagcctg agcccgtgga caaggtcccc ctctcgtaca cgcacgccta cggaggcagg
     1081 gaccatgcgg cggagcggac gcgcggaaac ccgttcctgg ccctgcacgg cgccgtcgac
     1141 ccgggggagt gcgatctcga catggcgagc ccgtttctct acccgcgcaa cccctgcggc
     1201 acgggttacc tcatcgagaa ggacgggagg gccgtcgagg cgctcaggct gccgaacctc
     1261 gaggacccgc aggaccccct gacgcccgag aggctctgcg tgcaggatca cctcaggtgg
     1321 ccgctcatgc ctctgcccca ggcgatggac tggtacgact acgggtggtt cccccgcatc
     1381 gcgtgcgccg ggatcgtgcc tgatcacgac gaggcggcgg cggtgttcgc ggaggtggac
     1441 aggggcctcg tccccgcatg ggtcgtcaag gacaggaccg tcgatgcgca cggtgcattc
     1501 ctgctcgcct cgggcgcctc accggggctg agcgtaccct acctcgtggg cggggaggag
     1561 atcctgctcg gcaacatgca cccggggcgg gaaacgatgc gcatacggct cccgaagaag
     1621 gcccctcgcc tgtttgcgga cgcgaggaac ggcaggttga accggacgga cgtggtcatg
     1681 cacaccgtgg tgatcgagcc cgaccgggac agggtctgca tcgtgtggcg gggaagcgcg
     1741 cctgcgctca ggccgtacct gccccaggag ctcgcaggaa tgcctttcat ggccgagtgg
     1801 ccgtgaagac aaagccgtac aaaggagtga acccatgagt ttcagccagg agaaccgcat
     1861 gctctcgatc gagacccccc tgggccccga tgtcttcgcg ctcacctcgt tcaggggaag
     1921 ggagggcata tcggt
//
LOCUS       DAOSCR010000537         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39350_length_1934_cov_4.177754, whole genome shotgun sequence.
ACCESSION   DAOSCR010000537 DAOSCR010000000
VERSION     DAOSCR010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39350_length_1934_cov_4.177754"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1430)
                     /gene="dnaA"
                     /locus_tag="PLT69_11290"
     CDS             complement(84..1430)
                     /gene="dnaA"
                     /locus_tag="PLT69_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOJ15067.1"
                     /translation="MDHLAMIKDSILLRAKGTPDAALLEQVSFTEEGDTVVVECPSVF
                     AQKYLGKMYRDLITQAARSHLDRKVDVDFRVTDPAKQDHGSSRTMDRTPVQMSLPACS
                     EPQASTGLNAHFTFDEFVVGSCNAFAYEAAQAVSRGEWARYNPLFFYSDIGLGKSHIA
                     HAIGNDVLSRKPRTPVRFVSARDFAHHYVNSVRNDCMKTFRNLYSSASMDVLFIDDVH
                     LFKNKEKTQLELCHVLDDLIGAGKQVILSGFRPPASIPHIENGLRSRLSSGLSINIKR
                     PDRDTRARIVLHKANKNGVTIPDEVVDFISENVHSNIRDLESAVLTISAMSSLMKRPV
                     SMDLARELLDGTFKKQQTMTIPAIMSLVAKSFSVHTEQLVSPSRKKEIVYPRQVAIYL
                     CRRYTGEPLQAIGEAFMRKHSSVIHSLETIENRYTCDLAVKRDIDYLVEKIESELA"
     gene            complement(1694..>1934)
                     /locus_tag="PLT69_11295"
     CDS             complement(1694..>1934)
                     /locus_tag="PLT69_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HOJ15068.1"
                     /translation="GLKDTVKALGETDRPCGIRAASPSAVDLEHAVREAVRIYTQDKA
                     LLNAMISNAMEQDVSWDAPSSEYADLYRICKKREK"
BASE COUNT          363 a          557 c          592 g          422 t
ORIGIN      
        1 agttcttcac cgccgttgac aacttccgcg accagagcag gcgcgcctcg ggtgtcattt
       61 gaggccggcc ggttgcgtga ggctcacgcg agctcgctct ctatcttctc gaccaggtag
      121 tcgatgtcgc gcttgacggc caggtcgcag gtgtagcggt tctctatggt ctcgagggag
      181 tggatgaccg aggagtgctt cctcatgaac gcctcgccta tggcctggag cggttcgccg
      241 gtgtagcgcc tgcacaggta tatcgccacc tggcgcgggt acacgatctc cttcttgcgg
      301 gagggcgaga cgagctgttc cgtgtggacc gagaagctct tcgccacgag gctcatgatc
      361 gcggggatcg tcatcgtctg ctgcttcttg aacgtcccgt cgaggagctc tcgggcgagg
      421 tccatcgaca ccggccgctt catgagggag ctcatggcgg agatggtcag caccgcgctc
      481 tccaggtcgc ggatgttcga gtgcacgttc tcgctgatga agtcgacgac ctcgtcgggt
      541 atggtcacgc cgttcttgtt cgccttgtgc aggacgatcc tcgccctggt atccctgtcg
      601 ggccgcttga tgttgatgct caggcccgat gacagccggg aacgcagccc gttctcgata
      661 tgggggatgg aggcgggggg cctgaacccc gacaggatga cctgcttgcc ggcgccgatg
      721 aggtcatcca gcacatgaca caactcgagc tgcgtcttct ccttgttctt gaaaagatgc
      781 acgtcgtcga tgaacaggac gtccatcgag gcgctgctgt agaggttcct gaaggtcttc
      841 atgcagtcgt tgcgcaccga gttcacgtag tggtgggcga agtcccttgc ggacacgaaa
      901 cgcaccgggg tccttggttt tcgggacagg acgtcgtttc ctatcgcgtg cgcgatgtgg
      961 ctcttgccga gcccgatgtc agagtagaag aagagggggt tgtacctggc ccactcgccc
     1021 ctggagacag cctgggccgc ctcgtatgcg aaggcgttgc agctccccac gacgaactca
     1081 tcgaacgtga agtgtgcgtt gagcccggtc gaggcctggg gctcagagca tgcgggcagg
     1141 ctcatctgca ccggggtgcg gtccatggtg cgggatgaac cgtgatcctg cttggcaggg
     1201 tccgtcaccc tgaagtccac gtccaccttt ctgtcgaggt gcgatctggc ggcctgggtg
     1261 atgaggtccc ggtacatctt gccgaggtac ttctgcgcga acacggatgg acactcgacg
     1321 acgaccgtgt ccccttcctc ggtgaacgag acctgctcga gaagagccgc atccggtgta
     1381 ccctttgccc gtaacaggat ggaatccttg atcattgcaa gatggtccac gcaatccccc
     1441 actgagatga acctcaatca acgcccgcca tgatgaccgc gggtcggtca aacgtcaaag
     1501 gcaaaccatg gccgttatcc tgagaagagg tttcttttgg ggaattctcg aatacgcgag
     1561 aaatatcaac gagataccaa aaaaaagaac tttggatctc atttcgggac aaagtgttca
     1621 ggagatcgga tccaccacag cccggccggg ttatcgacaa aatgtcgata agtgaggagc
     1681 tttcgatatc ggctcatttc tctcgttttt tgcaaatacg atacagatcc gcgtactcac
     1741 tggaaggggc atcccagctc acatcctgtt ccatcgcatt ggatatcatg gcattcaaga
     1801 gggccttgtc ttgtgtgtag atcctcacgg cttcccgaac cgcgtgctca aggtccaccg
     1861 ccgaggggga ggccgcccgt atgccgcagg gcctgtccgt ctcgcccaag gccttcaccg
     1921 tgtccttgag gccg
//
LOCUS       DAOSCR010000538         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39356_length_1934_cov_2.864822, whole genome shotgun sequence.
ACCESSION   DAOSCR010000538 DAOSCR010000000
VERSION     DAOSCR010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39356_length_1934_cov_2.864822"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1770)
                     /locus_tag="PLT69_11300"
     CDS             complement(322..1770)
                     /locus_tag="PLT69_11300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ15069.1"
                     /translation="MFWQVIAANKRNTVILVVAMGVVLALVGFCAGSLVGAWLGMTAY
                     GLEPAPGGPNAGWVGMVIALLVWFVLLLAALLGSDELFLGLAGAKEISHDTYPQLFNV
                     VEEMKIASSLPTMPRVFIVDDPAPNAFALGKSPKKCAVCVTAGLLAMCDRDELQGVIA
                     HEIGHIVNRDALYLGVAATMLGAVTIISDTLLRSLRYAPVARYRSPGTRGGKGMLNVW
                     FILLAFVFIIVSPILSSILYFSISRRREYLADATSARLTRYPPGLASALEKIMLSPAV
                     LTTAPRVIAPFYTVNPYKQDLEGGLFATHPPLPQRIRILRAMAGGASFRDYVKAYWDV
                     TRTRKQLIPVADMRLGETVEIRAPSPMTPEQGRTGDIIRAMNDFVFIPCPCGMTMKIP
                     PEFARGVVQCPRCGRDHTVKGPDKGSAAAILAGASAFTKQASGREQAERRVKGAWQTV
                     TCLGCGKGITLSSAFSLGTVTCTRCGQVIRLT"
     gene            complement(1778..>1934)
                     /locus_tag="PLT69_11305"
     CDS             complement(1778..>1934)
                     /locus_tag="PLT69_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ15070.1"
                     /translation="RQAYNDAALLLNNKVEMFPSNIVAKLFAFTKEPFFEMEDSSERA
                     VPKVSFS"
BASE COUNT          332 a          632 c          666 g          304 t
ORIGIN      
        1 aagcgctacc tcgaggccct tgaggctcgt ctcgggcccg cacgagaggg cgtgtgaggc
       61 gcgagaagag ggccgccgcc ctttgccgct ccctgcgaac ggcaaaggac gaaccaagag
      121 aggacgcctg atcctgggtg aaagacggtc ccctcatcga tgacggcacg aaccaaaaac
      181 cgccgtgccg gaaggccggg catgcagacg acccgtccct gcagggccgg acgaaggtga
      241 agagaccgcc tccttgtcct catcccgggc ggtgcgctcg agggaggcgg tattcttgct
      301 ttcgtttttt aggcctcggg gtcaggtcag cctgatgacc tgcccgcacc gcgtgcacgt
      361 gaccgtgccc aggctgaagg ccgaagacag ggttatgccc ttgccgcacc ccaggcacgt
      421 gacggtctgc cacgccccct tgacacgccg ctcggcctgc tcacggccgc ttgcctgctt
      481 ggtgaaggcg gacgccccgg cgaggatcgc cgcggccgaa cccttgtcgg ggcccttgac
      541 ggtgtgatcc ctgccgcacc tcgggcactg cacgacgccc cgtgcgaact cgggcgggat
      601 cttcatggtc atgccgcacg gacaggggat aaacacgaag tcgttcatgg cccggatgat
      661 gtcgcctgtc ctgccctgct ccggggtcat gggcgagggg gcgcgtatct ccacggtctc
      721 gcccagccgc atgtccgcca ccggtatcag ctgcttcctc gtgcgcgtga cgtcccagta
      781 ggccttcacg tagtccctga aggacgcgcc cccggccatg gccctcagaa tccggatccg
      841 ctggggcagc ggcgggtgcg tggcgaacag gccgccctcg aggtcctgct tgtaggggtt
      901 caccgtgtag aagggcgcga tcacccgcgg cgccgtggtg aggacggccg gtgagagcat
      961 gatcttctcg agggccgatg cgagcccggg cgggtaccgg gtgaggcggg cgctcgtggc
     1021 gtccgccagg tactccctcc tcctcgagat ggagaaatag aggatgctgg acaggatggg
     1081 gctcacgatg atgaacacga aggccagcag gatgaaccac acgttcagca tgcccttgcc
     1141 cccccttgtc ccgggcgagc ggtagcgggc cacgggcgcg tagcgcagcg accggagcag
     1201 cgtgtccgag atgatggtca ccgcgccgag catggtggcg gcgaccccca aatagagcgc
     1261 gtcgcggttg acgatgtgcc cgatctcgtg ggcgatgacg ccctggagct cgtcccggtc
     1321 gcacatggcg agcaggccgg cggtgacgca caccgcgcac tttttcggcg acttgcccag
     1381 ggcgaacgcg ttgggcgccg ggtcgtccac gatgaagacc ctgggcattg tgggcaggga
     1441 cgaggcgatc ttcatctcct ccaccacgtt gaagagctgg gggtaggtgt cgtgggagat
     1501 ctccttggcg ccggccaggc ccaggaacag ctcgtccgaa cccagaaggg ccgcgagcag
     1561 gagcacgaac cagacgagga gcgcgatgac catgcccacc cagcccgcgt tcgggccgcc
     1621 gggagcgggt tcgagcccgt acgcggtcat gcccagccac gcgcccacca gcgagcccgc
     1681 acagaagccc acgagcgcca gcaccacccc catggcgacg acgaggatga cggtgttgcg
     1741 cttgttggcg gctatgacct gccagaacat gggcctctca gctgaagctc accttgggca
     1801 cggcgcgctc ggaggagtcc tccatctcga agaagggctc cttggtgaag gcgaacagct
     1861 tggccacgat gttcgagggg aacatctcca ccttgttgtt caacaggagc gccgcgtcgt
     1921 tgtaggcctg gcgg
//
LOCUS       DAOSCR010000539         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39363_length_1934_cov_2.573709, whole genome shotgun sequence.
ACCESSION   DAOSCR010000539 DAOSCR010000000
VERSION     DAOSCR010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39363_length_1934_cov_2.573709"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLT69_11310"
     CDS             <1..299
                     /locus_tag="PLT69_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15071.1"
                     /translation="ELGRWSVSEGGMCLRNDSETLYVTDQGRYFIVYEGGMNASFHRL
                     PGVETWFGGSHTREVTIEEAVAWCEETGNDDALKGHLPFLVFLFERAPGSSHPA"
     gene            complement(761..1294)
                     /locus_tag="PLT69_11315"
     CDS             complement(761..1294)
                     /locus_tag="PLT69_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOJ15072.1"
                     /translation="MELKRLFVLAVCALAPFLSSPCEASRAYVGVQVGPEIVSDSEAD
                     LETALGFGVYAGYRIDRMLGIEASLTTADHDPDGRGADGLEITSIIVGPRINAQAGSA
                     RVYADVGLGLYFLDFESRVGDDTESSGGISMGAGIEVPLQRSMSLGMDLKYHVLFDDD
                     DLDSDLVTLMVRLGFDL"
BASE COUNT          328 a          653 c          612 g          341 t
ORIGIN      
        1 ccgaactggg gcgctggagc gtctccgagg gcgggatgtg cctcaggaac gattcggaaa
       61 ccctctacgt gaccgaccag ggcaggtatt tcatcgtgta cgaagggggg atgaacgcct
      121 ccttccaccg gctccccggg gtggaaacgt ggttcggcgg gagccacact cgagaggtga
      181 cgatcgagga ggcggtcgcg tggtgcgagg agacgggcaa cgacgacgcc ctcaaggggc
      241 acctgccctt cctggtcttc ctcttcgagc gggcccccgg ctcgtctcac ccggcctagc
      301 gggccttcgg ccgctgcccc ccttcgtgtt ccccttgcga gaggagccgc ccctgccgca
      361 gacgcgggga gaccatggcg ggacagaagc ctgacgccct cggggttcgg gtcatgtccg
      421 aatgcgactg ctttgcgggt tctccggatc gaggcggccc gtgcggccac ggggtaagca
      481 ccggcagctc cgcatgcgcc gcggtaagtc gccgggacct ggggtgagcc cacgaacgtg
      541 ccgcatgtcg aggtcttccc cgcctgccgg gtcctcaccg atagcgacac gagccgacct
      601 cccatgaccg tcgaggaggg ccgtgggatt caggaagggt cgcatgaagg aggccgtgca
      661 ggaccgcggc cgaggggccg gcacacgcgc ctggtgaatt ctcctggaag agaccccgct
      721 ggagcgcgtt cttggggtct tttcccgtgg gtgattggtg ctacaggtcg aacccgagcc
      781 ttaccatgag cgtcacgagg tcgctgtcga ggtcgtcgtc atcgaagagc acgtggtact
      841 tgaggtccat gccgaggctc atggaccgct gcaggggcac ctcgatgccc gcgcccatgg
      901 agatgccgcc agagctctcg gtgtcgtccc cgaccctgct ctcaaagtcg agaaagtaga
      961 gcccgagacc gacgtcggcg tacacccgcg cggagcccgc ctgcgcgttg atcctcgggc
     1021 cgacgatgat cgaggtgatc tccaggccgt cggcgcccct gccgtctgga tcgtggtcag
     1081 cggtggtcag ggaggcctcg atgcccagca tcctgtctat ccggtacccc gcgtagacac
     1141 cgaaaccgag agccgtctcc aggtctgcct cggagtccga cacgatctcg ggtccgacct
     1201 gcacccccac gtacgcccgg gaagcctcgc acggggatga aaggaaaggg gccagtgcgc
     1261 agacagccaa gacgaagagc cttttcagtt ccatggtgaa caccctccgg gacacgcttt
     1321 catggtgaac aatggatgaa acaggtcgag tcggtgattg acgatccatg tgaacaccct
     1381 ccgatataca tgcttccatg ggtgaccgtc gttttcttga gcctcgctcg tgatccgccg
     1441 tctccccagg ctttgtgctt aacagggctc ggagacgaag gtcaaggcaa ttcaggggaa
     1501 gtctctccct cggcgcccga gcctctcgtg ataccgcgtc ggcggagagc cacaggaccc
     1561 gaacccgcac ctggttgttc ccgccgctcg gcctgttctg ccccgggctc agcccaaact
     1621 gctggtccgc cccgaacatc gccatcagcc cctggaactt cgagacaacg gtttctgggt
     1681 tctgctggat catctgaccc agttggccac gcccctgcat ctcaagctgg gcgccctgga
     1741 tcacccccat ctgccggagg ttggcgctga actcacgaga acgctgggcc atggccagta
     1801 cattccccct cccctgactg atctgcccct ccatgcccgc cgcggccatc tgcgtctggg
     1861 cgatattccc ccgcagcccc gccaggaact cctgccccct cgtcacgaga tgtgccatcg
     1921 cactcgccac ctgg
//
LOCUS       DAOSCR010000540         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39381_length_1933_cov_3.641108, whole genome shotgun sequence.
ACCESSION   DAOSCR010000540 DAOSCR010000000
VERSION     DAOSCR010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39381_length_1933_cov_3.641108"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PLT69_11320"
     CDS             complement(<1..1165)
                     /locus_tag="PLT69_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOJ15073.1"
                     /translation="MAPLLIPAYRAAASVLAWPAAWALRKHPNFKGTILQRLGIHPPA
                     PSPGSNTLWVHASSVGEVRAVSLLVRAIKASRPGLEIWMSCMTATGREVATATEGVDH
                     VFGLPFDTRRAASRHALRLMPKALVVVETEIWPNLFLAHRRLGIPVVLVNARLSRRSE
                     RTYAPFRKALSHVLETVEVLAMSEVDARRFRSLGARNVRVLGNLKLDQVFETDHARAR
                     ALEEELHAHGRPVFVAGSVREGEEGLVLDAVLDIAGRVPGLLAVVAPRHPSRIEHLTG
                     IAESRGIRWTLRSRPRQDADLVVVDTMGELFTLYGIARVAFVGGSLVDCGGQNILEPV
                     AWGVPTVHGPFMDNFSWALEAIGNRTVTVKDARELSQAVTAMLTSLAPLTVASI"
     gene            complement(1150..1866)
                     /gene="kdsB"
                     /locus_tag="PLT69_11325"
     CDS             complement(1150..1866)
                     /gene="kdsB"
                     /locus_tag="PLT69_11325"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOJ15074.1"
                     /translation="MGVVAVIPARYASTRFPGKPLVDVCGKPMIRWVYEQVKGCASID
                     RVIVATDDERIKDAVASFGGDAVMTPADCPSGTDRVALAIRDMDCAYIINVQGDQVLL
                     DQASLDGLVGSLRQGSPMATIAAKASEEDASNPDCVKVVCSRSGYALYFSRSAIPFAR
                     NPGNAPMLRHIGIYGFSRDALTLFTSLEPSPLERTESLEQLRALENGIPIRVIVAEGM
                     FHEVNSPKDLEGLPRPWPLS"
BASE COUNT          314 a          642 c          664 g          313 t
ORIGIN      
        1 tgatcgacgc gacggtcaag ggcgccagtg aagtgagcat ggcggtgacc gcctggctga
       61 gctccctggc gtccttgacc gtgacggtgc ggttgccgat cgcctccagg gcccaggaga
      121 agttgtccat gaagggccca tggaccgtcg ggacccccca tgccacgggt tcgagtatgt
      181 tctgcccccc gcagtccacg agtgacccgc ccacgaaggc gacacgggca atgccgtaga
      241 gggtgaagag ctcgcccatg gtgtccacga cgacgaggtc cgcgtcctgc cggggtctgc
      301 tcctcagggt ccacctgatg cccctgctct cggcgatccc ggtgaggtgc tcgatcctcg
      361 aagggtgcct gggcgccacg accgccaaga gccccgggac gcgcccggcg atgtcgagga
      421 cggcgtccag cacgaggccc tcctcgccct cgcgcacgct gcccgcgacg aagaccggcc
      481 tgccgtgcgc atggagctct tcttcgaggg cccgcgcccg cgcatggtcg gtctcgaaga
      541 cctggtccag cttgaggttc ccgagcaccc tgacgttccg tgcgcccagg gagcggaaac
      601 gtcgcgcgtc cacctcggac atggcgagca cctccacagt ctcgagcacg tgggagaggg
      661 ccttcctgaa cggtgcgtac gtcctctcgg aacgcctgga caggcgtgcg ttcaccagga
      721 ccacggggat gcccagccgc ctgtgggcga ggaacaggtt cggccagatc tccgtctcca
      781 ccaccaccag ggccttcggc atgagcctga gcgcgtgtct ggaggcggca cgccgggtgt
      841 cgaagggaag gccgaagacg tggtcgaccc cttcggtggc cgtggccacc tcgcggcccg
      901 tggcggtcat gcaggacatc cagatctcga ggccgggcct ggaagccttg atggccctca
      961 ccagcaggga taccgctctc acctccccca cggacgaggc gtgcacccag agcgtgttcg
     1021 acccgggcga cggcgcgggc ggatgtatcc ccagcctctg gaggatcgtg cccttgaagt
     1081 ttgggtgctt cctgagcgcc caggcagcag gccaggccag gaccgaggcc gcagcccggt
     1141 atgcagggat caggagaggg gccatggccg gggaagccct tccaggtcct tcggggaatt
     1201 gacctcgtgg aacatgccct cggccacgat gacccggatc ggtatgccgt tctcgagcgc
     1261 cctgagctgc tcgagcgact cggtgcgctc gagtgggctg ggttccaggg aggtgaagag
     1321 tgtcagcgcg tccctcgaga acccgtagat cccgatgtgc ctgagcatgg gggcgttccc
     1381 ggggttccgc gcaaacggta tggcggatcg cgagaagtaa agggcatacc ccgagcgcga
     1441 gcacaccacc ttcacgcagt cggggttcga tgcgtcctcc tcggaggcct tcgccgcgat
     1501 ggtggccatg ggggagccct ggcggagcga gcccacgagg ccgtccagcg atgcctggtc
     1561 gaggaggacc tggtcgccct gcacgtttat gatgtacgca cagtccatgt cgcgtattgc
     1621 gagggccacg cggtcggtcc ccgaggggca gtcggcaggg gtcatcaccg cgtccccgcc
     1681 gaaggaggcc acggcgtcct ttatcctctc gtcgtcggtg gcgacgatga ccctgtcgat
     1741 ggacgcgcag cccttgacct gctcgtagac ccacctgatc atgggtttgc cgcaaacgtc
     1801 aacgaggggt ttgcccggga accgggtgga tgcgtacctg gctgggatga cggccacgac
     1861 gcccatgtca gccctccccc gagaccaagc cgagttccct gagtgcgcgc aggtgctccc
     1921 tgtacgaaga ctt
//
LOCUS       DAOSCR010000541         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39633_length_1925_cov_3.223529, whole genome shotgun sequence.
ACCESSION   DAOSCR010000541 DAOSCR010000000
VERSION     DAOSCR010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39633_length_1925_cov_3.223529"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLT69_11330"
     CDS             complement(<1..1122)
                     /locus_tag="PLT69_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ15075.1"
                     /translation="MRESILLDPHTRRKNFGKRQTALEMPDLVELPKSSYSAFLQKDV
                     PPEKRRNIGLQQVFNSVFPIKDFNEDASLEFVEYRLGEPKYDMEECIQKGLTYAAPVT
                     LKVRLIIFDRDEESGTQSIRDIKEQEVYFGEIPLMTPSSTFIINGTERVIVNQLHRSP
                     GIFFDETTARAATGGRKLYSARIIPARGSWLDLDFDAKNILYLRIDRRRKMPVTTLLK
                     ALGYTNQDILGVYYPKERYTIEGEEAHRHVDIESLVGLKTDIDLKLKDSDEIVVKAGK
                     KVTPKTAHKYRDKVFEPVVLKKEDLLGKVLAEDIVNMETGEIIAEANDEITPQLLERL
                     EAMGIREIKTIFYDEISYSSSLSKTLALDKVDTVDEALIE"
     gene            complement(1228..1617)
                     /gene="rplL"
                     /locus_tag="PLT69_11335"
     CDS             complement(1228..1617)
                     /gene="rplL"
                     /locus_tag="PLT69_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ15076.1"
                     /translation="MSQITREDVIKFIESMTVLELAEFVKELENKFGVTAAAPVAAVA
                     AMPAAGAPAAAEVEEKTAFDVVLKEAGPQKIQVIKVVRELTSLGLKEAKDLVEGAPNK
                     VKEGISKEEAEEAKKKLEGVGAVVEIK"
     gene            complement(1621..>1925)
                     /gene="rplJ"
                     /locus_tag="PLT69_11340"
     CDS             complement(1621..>1925)
                     /gene="rplJ"
                     /locus_tag="PLT69_11340"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ15077.1"
                     /translation="VGPTAVAVALGDPVAVAKALVNFTKENEQFGIKGGVLGGRPIKA
                     SDIMELAKMPPREVLLARMLGSLNAPVSNFVGVLAAIVSQLVYVLKAIENKKREGE"
BASE COUNT          291 a          634 c          556 g          444 t
ORIGIN      
        1 ctctatgagg gcctcgtcga ccgtgtccac cttgtcgagg gcgagggtct tgctgagcga
       61 agagctgtag gagatctcgt cgtagaagat cgtcttgatc tccctgatgc ccatggcctc
      121 gagcctttcc aagagctggg gggtgatctc gtcgttcgcc tcggcgatga tctcgccggt
      181 ctccatgttc acgatgtcct cggccaggac cttgcccaac aggtcctcct tcttgaggac
      241 cacgggctcg aagaccttgt ccctgtactt gtgggccgtc ttgggcgtca ccttcttgcc
      301 ggccttcacc acgatctcgt ccgagtcctt gagcttcagg tcgatatcgg tcttcagccc
      361 gacgaggctc tcgatgtcca cgtgcctgtg cgcctcttcg ccctctatgg tgtaccgctc
      421 cttggggtag tagacgccga ggatgtcctg gttcgtgtac cccagggcct tcaagagggt
      481 ggtcaccggc atcttccttc gcctgtctat cctcaggtag aggatgttct tggcgtcgaa
      541 gtcgaggtcg agccaggagc ccctggcggg tatgatgcgg gccgaataga gcttcctgcc
      601 gcccgttgcc gccctcgccg tggtctcgtc gaagaatatg cccggcgagc ggtggagctg
      661 gttgacgatg acgcgctcgg tcccgttgat gatgaaggtg gaactcgggg tcatgagcgg
      721 gatctcgccg aagtagacct cctgctcctt gatgtcgcgg atgctctggg tgcccgactc
      781 ctcgtcgcgg tcaaagatga tgagcctcac cttgagggtc accggcgcgg cgtaggtcag
      841 ccccttctgg atgcactcct ccatgtcgta cttgggctct ccgaggcggt actccacgaa
      901 ctccaaggat gcgtcctcgt tgaaatcctt gatgggaaaa accgagttga acacctgctg
      961 cagaccgatg ttcctcctct tttccggcgg gacgtccttc tgcaggaatg cgctgtagga
     1021 cgacttcggg agctcgacca ggtcgggcat ctcgagcgcg gtctgtcgct tgccgaagtt
     1081 cttccgcctc gtgtgcggat ctaacaggat gctctctctc atcttcctcg tccacctcgt
     1141 atttggctgt gtgtgatgct ctccccaggg caagaagggg ggacaggcat gccccttccc
     1201 ccctgatatc cacctttcca ggtgacgtta cttgatctcg acgacggcgc ccaccccctc
     1261 gagcttcttc ttcgcctcct cggcctcttc cttgctgatg ccttccttga ccttgttggg
     1321 ggcgccctcg accaggtcct tcgcctcctt gaggccgagg ctcgtcagct cgcgcaccac
     1381 cttgatgacc tggatcttct gcgggccggc ctccttgagc acgacgtcga aggccgtctt
     1441 ctcctcgacc tcggccgccg ccggggcccc ggccgccggc atcgcagcca ccgccgccac
     1501 cggcgccgcc gccgtgacac cgaacttgtt ctcgagttcc ttgacgaact cggcgagctc
     1561 cagaaccgtc atgctctcga tgaactttat gacatcctca cgtgtgatct gggacatgtt
     1621 tcactctcct tcgcgtttct tgttttcgat tgccttgagc acgtagacca gctgcgagac
     1681 gatcgcagcg agaaccccga cgaagttgga caccggcgcg ttcagcgagc cgagcatgcg
     1741 cgccagcaac acctccctgg gtggcatctt ggcgagctcc atgatgtccg acgccttgat
     1801 gggacgcccc cccaggacgc cgcccttgat gccgaactgt tcgttctcct tggtgaaatt
     1861 caccagggcc ttggccaccg caacggggtc gccgagcgcg accgccaccg ccgtgggccc
     1921 cacga
//
LOCUS       DAOSCR010000542         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39705_length_1923_cov_2.351713, whole genome shotgun sequence.
ACCESSION   DAOSCR010000542 DAOSCR010000000
VERSION     DAOSCR010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39705_length_1923_cov_2.351713"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLT69_11345"
     CDS             <1..819
                     /locus_tag="PLT69_11345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOJ15078.1"
                     /translation="LSGGEALARRDVMELAQDGTRLGLRMTLATNGSLVDRQAARLIK
                     ASGIARVSVSLDGAHASTHDTIRGMPGAFDMAIQGISRLVEAGVPVQVNTTVASVNIS
                     EMSRIPELVEKLGACAWHVFFLVPTGRGASMEPALVGEYRDMLEDFRKVSNHSSIECK
                     ATCAPQYYRMLLEEEGFSPTKGCLAGTGFGFVSATGEVQPCGFLQVSCGNVRQRPFSE
                     IWVSSPLLLALRDPDVLKGKCGSCRFKEVCGGCRARAFEVLGDALETDPICWFS"
     gene            829..1275
                     /locus_tag="PLT69_11350"
     CDS             829..1275
                     /locus_tag="PLT69_11350"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOJ15079.1"
                     /translation="MDDIDRAILSRISRSIDLVPRPFQGLADMLGVSEEEIAGRIVRL
                     KEEGVIRRVGAVIDPSRLGWVSTLCAVDLPEDGIDEFARIVSGFDEITHNYVRDGRPN
                     CWFTVIAPSRARIDEIRSEISSALGADVMDMPARRVFKIGVSFGLE"
     gene            complement(1303..>1923)
                     /locus_tag="PLT69_11355"
     CDS             complement(1303..>1923)
                     /locus_tag="PLT69_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15080.1"
                     /translation="RCPSCLDSGQGGSAGGEEAGDHWFTCPSAQRCNRETAVDTTNEG
                     EGMISEKIVQLIKDNADTLTNRLCKDLLTREETKGYRKLDRDIVYDRVYDVYSRLDSW
                     LAGDKKKAELKDHYKKLGMTRFHEGIPLSDVVMALMLIKRHLWLYVQETQFFDSSFQL
                     WQALEFNNRVVLFFDRAIYFTCLGYQEEEEKCLGKPKEGFISRVFK"
BASE COUNT          325 a          609 c          621 g          368 t
ORIGIN      
        1 ctgtccggag gagaggcgct cgcccgccgt gacgtgatgg aactcgcgca agacggcacc
       61 cgcctcgggc tccgcatgac cctcgccacc aacgggtctc tcgtggaccg gcaggcggcc
      121 cggctgatca aggcgtctgg catcgcgcgc gtgtccgtga gcctcgacgg cgcccacgcc
      181 tcgacgcacg acaccatccg cggcatgccg ggcgcgttcg acatggccat acagggtatc
      241 tcccgcctcg tcgaggcagg ggtcccggtc caggtcaaca ccacggtggc ctcggtgaac
      301 atctccgaga tgagcaggat tccggagctc gtcgagaaac tcggcgcctg cgcgtggcac
      361 gtcttcttcc tcgtccccac gggaaggggc gcgtccatgg agcccgccct cgtgggcgag
      421 taccgggaca tgctcgagga cttcaggaag gtctcgaacc acagcagcat cgagtgcaag
      481 gcgacctgcg ccccccagta ctacaggatg ctgctcgagg aggaaggctt cagccccacg
      541 aaggggtgtc tcgccggcac gggtttcggc ttcgtctccg ccacgggcga ggtgcagccg
      601 tgcggcttcc tccaggtctc ctgcggcaac gtgagacaga gacccttttc ggagatctgg
      661 gtgtcctccc cgttgctcct tgccctcagg gacccggacg tgctcaaggg caagtgcggg
      721 tcgtgcaggt tcaaggaggt ctgcggaggg tgcagggcca gggcgttcga ggtgctcggg
      781 gacgcgctcg agacggaccc gatctgctgg ttctcatgag ggcgtgccat ggacgacatc
      841 gaccgggcca tcctgtcacg catcagccga tccatcgatc tcgtaccgag gccgtttcag
      901 ggcctggccg acatgctcgg cgtgagcgag gaagagatcg caggccgcat cgtgaggctc
      961 aaggaagagg gggtcatcag gcgcgtcggt gcggtcatcg acccctctag gctgggctgg
     1021 gtctcgacgc tgtgcgccgt ggaccttccc gaggacggca tcgacgagtt cgcccggatc
     1081 gtctccggct tcgacgagat cacccacaac tacgtgcggg acggcaggcc gaattgctgg
     1141 ttcaccgtca tcgcaccgag cagggcccgc atcgatgaga tcaggtcgga gatatcgagc
     1201 gccctgggag cggacgtcat ggacatgccc gcaaggcgtg tgttcaagat cggcgtctcg
     1261 ttcgggctcg agtgagctcg aagcacgggc ctcagcgtat ccctacttga acaccctgga
     1321 tatgaacccc tccttgggct tgcccaggca cttctcttcc tcttcctggt agccgaggca
     1381 ggtgaagtat atcgcgcggt cgaagaagag gaccacccgg ttgttgaact cgagggcctg
     1441 ccaaagctgg aaggacgagt cgaagaactg cgtctcctgc acgtagagcc acaggtgccg
     1501 cttgatgagc atgagggcca tgaccacgtc gctcagcggg atgccctcgt ggaagcgcgt
     1561 catgccgagt ttcttgtagt gatccttgag ttcggccttc ttcttgtcgc ctgcgagcca
     1621 gctgtccaag cggctgtaca cgtcgtagac gcgatcgtac acgatatccc tgtcgagctt
     1681 gcggtatccc ttcgtctcct ctctcgtcag gaggtccttg cagagacggt tcgtcagggt
     1741 gtctgcgttg tccttgatga gctgaacgat cttctccgat atcatgcctt ccccctcgtt
     1801 ggtggtatcg acggcggtct ccctgttgca ccgttgagca gacggacatg tgaaccagtg
     1861 gtctcccgcc tcttcgcccc cggcggaccc tccctgtcca ctgtcaagac aggaggggca
     1921 tct
//
LOCUS       DAOSCR010000543         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39791_length_1920_cov_3.036461, whole genome shotgun sequence.
ACCESSION   DAOSCR010000543 DAOSCR010000000
VERSION     DAOSCR010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39791_length_1920_cov_3.036461"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="dapA"
                     /locus_tag="PLT69_11360"
     CDS             <1..790
                     /gene="dapA"
                     /locus_tag="PLT69_11360"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOJ15081.1"
                     /translation="DVIAGGVSGIVPCGTTGESATLTHEEHERLIDLTVDLVAGRVKV
                     IAGTGSNSTDEAVRLTRHAMKAGADAALLITPYYNKPTQKGLYEHYKQVAASCDIPLI
                     LYNVPGRTGVNLAAETTIELSKIDTIVGIKEASGDLVKCSQIVRDTPGDFAVLSGDDA
                     LNYPILCLGGKGAISVTANVLPSKVSTLCDAWLKGDAETARTIHLDLLEINQALFIET
                     NPIPVKTALAMMGKVREEFRLPLCPMSGANRERLASVLARYGCI"
     gene            813..1628
                     /gene="dapB"
                     /locus_tag="PLT69_11365"
     CDS             813..1628
                     /gene="dapB"
                     /locus_tag="PLT69_11365"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOJ15082.1"
                     /translation="MTRIGVVGIGGRMGSAVGRLVLAERDLELAGALEAPSHPFVGRD
                     AGEVLGVGAVGVRVSGQVDEAFGPCDCIVDFSLPAVTLGVARYASGARKAMVIGTTGF
                     TDEEKRVLEACAQRVPIVLSPNMSVGVNVLLRVVEELGRVLDDTYDAEIVEAHHRMKK
                     DAPSGTALALAQALAQGRGVELSQRARFSRQGIIGERPVGEIGIQTVRAGDIVGDHTV
                     IFAGPGERIEITHRAHTRENFARGAVRAALWITSRPAGLYTMKDVLGFEHGRR"
     gene            1615..>1920
                     /locus_tag="PLT69_11370"
     CDS             1615..>1920
                     /locus_tag="PLT69_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ15083.1"
                     /translation="MVVGRCITRTGERAWFCSEDRVTFRRWDPETFDKGGELAVERLL
                     APVEPGKIVAVGLNYRDHARELGLSLPDEPILFMKPSTSVIGPGDAIVHPPMAGRLDY
                     "
BASE COUNT          344 a          590 c          699 g          287 t
ORIGIN      
        1 ggacgtgatc gcaggcggcg tcagcgggat agtgccgtgc gggaccaccg gggagtcggc
       61 gacgctgact cacgaggagc acgaacgcct catcgatctc acggtggatc tcgtggcggg
      121 ccgcgtgaag gtgatcgcgg gtacgggctc caactcgacg gacgaggccg tgaggctgac
      181 gaggcacgcg atgaaggccg gggccgatgc cgccctcctg atcacgccct actacaacaa
      241 gcccacccag aaggggctct acgagcacta caagcaggtg gcggcgtcct gcgacatccc
      301 cctgatcctc tacaacgtgc ccggtcggac gggtgtgaac ctcgccgcgg agacgacgat
      361 cgagctctcg aagatagaca ccatagtcgg catcaaggag gcgagcggcg acctggtgaa
      421 gtgttcgcag atcgtgcgcg acacccctgg agacttcgcc gtgctctcgg gcgacgacgc
      481 gctcaactac cccatcctct gcctgggggg caagggcgcg atatccgtga ccgcgaacgt
      541 gctgccctcg aaggtctcga cgctctgcga cgcctggctg aagggggacg ccgagacggc
      601 gcgcacgatc cacctcgatc tgctggagat caaccaggca ctgttcatcg agaccaaccc
      661 gatccccgtg aagaccgccc tggcgatgat gggaaaggtc agggaggagt tcaggctccc
      721 cctgtgcccc atgtccgggg cgaaccgcga gaggctcgcg tccgtgctcg cccggtacgg
      781 atgcatctga aggggaaggc ccggagagac ccatgacgag gataggcgtg gtcggcatag
      841 gcgggaggat gggctccgcc gtcgggaggc tcgttctggc cgagcgtgac ctggaacttg
      901 caggcgctct cgaggcgccg tcccacccct tcgtgggcag ggacgcggga gaggtcctcg
      961 gcgtgggggc cgtgggcgtg agggtgagcg gtcaggtgga cgaggcgttc ggcccgtgcg
     1021 attgcatcgt ggacttctcg ttgccggccg tgaccctcgg cgttgcccgg tacgccagcg
     1081 gcgcgagaaa ggcgatggtc atcgggacca cggggttcac ggacgaggag aaaagggtcc
     1141 tggaggcctg cgcgcagagg gtccccatcg tgctgtcccc caacatgagc gtcggggtta
     1201 acgtcctgtt gagggtcgtg gaggaactcg gccgggtgct cgacgacacc tacgacgcgg
     1261 agatcgtgga ggcccaccac aggatgaaga aggacgcacc ctcggggacg gcgctggcgc
     1321 tggcacaggc cctggcacag ggcaggggcg tggaactctc gcagagggcc cggttttccc
     1381 gccaggggat catcggggag aggccggtgg gagagatcgg catccagacg gttcgggccg
     1441 gcgacatcgt gggcgatcac accgtgatct ttgcaggccc gggcgagcgc atcgagatca
     1501 cccacagggc gcacacccgc gaaaacttcg cgcgcggggc cgtcagggcc gccctgtgga
     1561 tcacgtccag gcccgccggg ctgtatacca tgaaggacgt actgggtttc gagcatggtc
     1621 gtcggtaggt gcatcaccag gacgggggag agggcctggt tctgctcgga agacagggtg
     1681 accttccgtc ggtgggaccc ggagaccttc gacaaggggg gggagctcgc cgtggagagg
     1741 ctcctggccc ctgtcgagcc cgggaagatc gtggccgtgg ggctcaatta ccgtgaccac
     1801 gcaagggaac tgggcctgag cctcccggat gagcccatcc tcttcatgaa gccgtccacg
     1861 agcgtcatcg gccccgggga cgcgatcgtc cacccgccca tggcaggccg actcgactat
//
LOCUS       DAOSCR010000544         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39847_length_1918_cov_3.194310, whole genome shotgun sequence.
ACCESSION   DAOSCR010000544 DAOSCR010000000
VERSION     DAOSCR010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39847_length_1918_cov_3.194310"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLT69_11375"
     CDS             complement(<1..627)
                     /locus_tag="PLT69_11375"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOJ15084.1"
                     /translation="MNRTSRSLLAALVACMLAPFPLLGAGLELMVSDKEASAHLEDVP
                     CVTVSPVARASLFVERTEGMVTASDGRVTYRAATLPEALDGYLRLMGVCVAKDVVIRG
                     TQRISNDAVRFRIKTAKGDIIHKGRIKKDIEEIYAMGYFEKCDASFEDDVVIFDVSEY
                     PVIMSIELKGNEEIKEKELLDAIGMKKFDILNTRQLKTAIERIKSQYRQ"
     gene            complement(608..1297)
                     /locus_tag="PLT69_11380"
     CDS             complement(608..1297)
                     /locus_tag="PLT69_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ15085.1"
                     /translation="MRGEATLRAHGLHKSFRKGPETIVVLRGVDLEVHRGEGVAVVGA
                     SGSGKSTLLHLLGGLEKPDSGTIAYGADDITAMDEASMARFRNSRIGFVFQFHYLLPE
                     FTALENVMLPALITSRVSAQVRKKAEGLLDMVGIAHRKTHKPGELSGGEQQRLALARA
                     LMMNPEVLLADEPTGDLDPETGLKIIELFGRLRKDLGVTLVVATHNPELARTMDRVLV
                     LKGGHLEPNIP"
     gene            complement(1287..>1918)
                     /locus_tag="PLT69_11385"
     CDS             complement(1287..>1918)
                     /locus_tag="PLT69_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ15086.1"
                     /translation="ITMNAAEKLFMRDAPSGVEVKVKDIYRAEEVARQALGRLGEGYW
                     AKTWMDMNKSLFSALRLEKTIMFIILMFIILVAAFSIVSTLIMLVMEKRKEIAILKSM
                     GATTRQILGIFVTVGMVIGTAGTAAGLALGLLLARNMNAVVRIVEFAFRIQVMPKDVY
                     YITGLPTAVDPVEVASIVAASLVLCFAATIYPARQASRQDPVEVIRYEG"
BASE COUNT          319 a          634 c          585 g          380 t
ORIGIN      
        1 ctggcggtac tggctcttta tgcgttctat cgcggtcttg agctgcctcg tgttgaggat
       61 gtcgaacttc ttcatgccga tggcgtcgag cagctccttc tccttgatct cctcgttgcc
      121 cttgagctcg atggacatga tcacggggta ctcgctcacg tcgaagatca ccacgtcgtc
      181 ctcgaacgag gcgtcgcact tctcgaagta ccccatggcg tagatctcct cgatgtcctt
      241 cttgatcctg cccttgtgga tgatgtcgcc cttcgcggtc ttgatgcgga agcggacggc
      301 gtcgttgctg atgcgttgcg tccccctgat cacgacgtcc ttcgcgacgc agacgcccat
      361 gaggcggaga tacccgtcca gggcctccgg gagggtcgcc gccctgtagg tcacccggcc
      421 gtcggacgcg gtgaccatgc cctcggtcct ctcgacaaag agcgaggccc gggccacggg
      481 cgacaccgtc acgcacggca cgtcctccag gtgcgcggac gcctccttgt ccgagaccat
      541 gagctcgaga cccgcgccaa gaagaggaaa gggtgcgagc atgcatgcga cgagcgcggc
      601 caggaggcta cgggatgttc ggttcaaggt gccccccctt cagtacgagt accctgtcca
      661 tggtgcgcgc gagctcggga ttgtgcgtgg ccaccacgag cgtcacgccg agatccttcc
      721 tcaggcggcc gaagagctct atgatcttga gcccggtctc ggggtcgagg tctcctgtgg
      781 gctcgtcggc caagaggacc tcggggttca tcatcaaggc cctcgccagt gcgagacgct
      841 gctgttcccc gccggagagc tcaccgggct tgtgtgtctt cctgtgtgct atccccacca
      901 tgtcgagcag cccctccgcc ttctttcgaa cctgggccga gacgcgggag gtgatgagcg
      961 ccggcagcat gacgttctcg agcgccgtga actccggcag gaggtagtgg aactggaaca
     1021 cgaaccctat gcgggagttt cgaaacctcg ccatggaggc ctcgtccatg gcggttatgt
     1081 cgtccgcgcc gtaggcgatg gtgcccgagt cgggcttctc gagcccgccc agcaggtgca
     1141 acagcgtgct ctttcccgat cccgaggcgc cgacgaccgc cacaccctcg ccgcggtgga
     1201 cctcaaggtc aacacccctg agcaccacga tcgtctccgg gcccttcctg aaagacttgt
     1261 ggaggccgtg cgcccgaagg gtcgcctcac ccctcatagc ggatcacctc cacagggtcc
     1321 tgcctggatg cctggcgggc cgggtagatc gtcgccgcga agcacaacac gagcgaagcc
     1381 gcgacgatcg atgccacctc gacggggtcc acggccgtgg gcaggcccgt gatgtagtac
     1441 acgtccttgg gcatgacctg gatcctgaag gcgaactcca cgatcctcac cacggcgttc
     1501 atgttcctcg ccagcagcag gccgagggcg aggcccgcag cggtaccggc cgtcccgatg
     1561 accatgccca cggtgacgaa gatccccagg atctgcctcg tggtggcgcc catggacttg
     1621 aggatggcta tctccttgcg tttttccatg accagcatga tgagggtgct cacgatgctg
     1681 aaggccgcca ccaggatgat gaacatgagg atgatgaaca tgatcgtctt ctcgagcctg
     1741 agcgcggaga agaggctctt gttcatgtcc atccaggtct tggcccagta cccttcgccc
     1801 agtcgcccga gtgcctgtct cgccacctcc tcggccctgt agatgtcctt caccttgacc
     1861 tccaccccgc tcggcgcgtc cctcatgaag agcttttccg cggcgttcat ggtgatgt
//
LOCUS       DAOSCR010000545         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39896_length_1916_cov_4.921010, whole genome shotgun sequence.
ACCESSION   DAOSCR010000545 DAOSCR010000000
VERSION     DAOSCR010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39896_length_1916_cov_4.921010"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1421..1597
                     /locus_tag="PLT69_11390"
     CDS             1421..1597
                     /locus_tag="PLT69_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15087.1"
                     /translation="MMTLWIVLGALFMTGIGIRFTYRVLGLTRVEAAAVYALVVLLVG
                     INTAPAREMIMRMF"
     gene            1645..1890
                     /locus_tag="PLT69_11395"
     CDS             1645..1890
                     /locus_tag="PLT69_11395"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ15088.1"
                     /translation="MKARTKREAMTRDVRNRVCRISGYSLVLVVMTFIGLYGGMWLDK
                     TLGMAPNFTLVGLVAGVCLGFKGFVEEVIIERKAKRA"
BASE COUNT          363 a          608 c          656 g          289 t
ORIGIN      
        1 gacgtcctca ccgggggcgg caaggcgcgt ggttgagacg ctgcgcctgg gtcggcacgc
       61 cccggcatgc ctcttgtgcg cctcaggcag cccgagcctg caggggtcag ccgggcgtct
      121 gggtccctcc cgcgtgcggg ccgaggcggt gttgactgtg atagggttcg atgaaggtca
      181 ggggcccctc ctgcgctcgg gcctggagaa gcccgagcgg caacaagagc tctttgatga
      241 gcagaggacc ctcgcgagcg agggattcga gaagggtgcc gttgcgctga gcaggtgcat
      301 gaacgaggcc tctcgcgcgc ggtccgcggc gaatcgtggc gctggcggac gagtacgacg
      361 cccgcggcct ccctcgcgcg ccggaagcgg gtcaccaacc cgcctgacag agaatcccct
      421 gatcgtggcc cctcccgcat gcgggcagcg gtgttctgcc gtattctctg aaggtcgata
      481 ggtcagaggc ccctcgagcc tgcgggctgc aaaaaactgg ggagttgaaa tgcgcgcacc
      541 agcgacagag gcccctcgcg cggaagctgc ggttcggctc gagggccacg acgaactcga
      601 catacagttg cacatcccac gggcgggccg cgggcatgga gatgaccgat cggtaaaaga
      661 gctcttggat gacttggatg aacagatgcc ccacgcccgc gcatgcgggc gtggaagcca
      721 cggccggggc agggtcatcg ccgggcccta aggagcgcgc aagcatatct cacggggagg
      781 gcttccccag cccgcatgca gcaagggccc cgcgcaggac cccgcatgcc cccacccggc
      841 tgacctcagc gtgcccccct cgaaccgagt acaggcggcc acacggttca agccgtcttt
      901 cctgcccatc cctcgtcctt cgcccaggcg gcctcgcgga taggccgggc atgacattcg
      961 gctacgggtg cagaggcagg tcctcactgg tcccggcaat gcacgcttgc gtttctcaag
     1021 gggaaggctt ccccagcccg catgcagcaa gggccccgcg caggaccccg cgtgccccca
     1081 cccgcctgac ctcagcgtga ccccctcgaa ccgagtacag gcggccacac ggttcaagcc
     1141 gtctttcctg cccatccctc gttccgcgcc taggctgaca cacggctagg ccggccgtga
     1201 cattctgtca cagcggccag gggtcagcgc aggggttcgt gacagattgt accaggtcga
     1261 aagaccgccc cacccccacg tgacaacatg gcaggcagca gcctcaccgg ctaggacacg
     1321 aaccaatgat atcatagagt tgtgagatag ccaggcctgg cacgcccctt gcctcagtgg
     1381 gagtggtacc gaaaaaagaa ccccgaggga gggaaagacc atgatgacac tgtggatcgt
     1441 actcggcgca ctgttcatga ccggcatcgg catcaggttc acctacaggg tgttggggct
     1501 caccagggtc gaggccgcgg cggtctacgc gctggtcgtg ctgctcgtgg ggatcaacac
     1561 ggcgccagcc cgagagatga tcatgaggat gttctaggca ggaaggacga gggacgaggc
     1621 aagcaccgca cgggaggtgg agccatgaag gcgaggacga agagagaggc catgacgagg
     1681 gacgtgagga accgcgtgtg caggatcagc gggtacagcc tggtgctcgt ggtcatgacc
     1741 ttcatcgggc tctacggcgg gatgtggctg gacaagacgc tgggcatggc gcccaacttc
     1801 acgctcgtgg gccttgtcgc gggcgtctgc ctcggcttca aggggttcgt ggaagaggtc
     1861 atcatcgaac gcaaggccaa gagggcctga gggaaagggc caccacggcg cacgga
//
LOCUS       DAOSCR010000546         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_39905_length_1916_cov_2.915099, whole genome shotgun sequence.
ACCESSION   DAOSCR010000546 DAOSCR010000000
VERSION     DAOSCR010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39905_length_1916_cov_2.915099"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1333)
                     /gene="gap"
                     /locus_tag="PLT69_11400"
     CDS             complement(317..1333)
                     /gene="gap"
                     /locus_tag="PLT69_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ15089.1"
                     /translation="MIRVGINGFGRIGRQVLKAIMERAGDTLQVVAINDLFDVNTNAH
                     LYMYDTNYGTFGGSVKVKGNSFVINGNTIKSFAMRDPANIPWDDEGVQIVIESTGLFT
                     SKTDAMAHIYGGAKKVIITAPAKQEDITVVMGVNHTKYSPTKHHVISNASCTTNCLAP
                     PALVVHKAFGIEKGWMTTVHAYTNDQRILDMPHKDLRRARAAAMSIIPTSTGAARALS
                     LVIPDLKGKVDGFALRVPTPTVSVVDLTVQLRKNTTTDDLRAVLKRASKTTLKGIMGC
                     EERPLVSVDFKGNELSSIVDIEYVRVMQDNLAKIVAWYDNEWGYSCRVADLARYIALK
                     GIPD"
     gene            complement(1351..1908)
                     /locus_tag="PLT69_11405"
     CDS             complement(1351..1908)
                     /locus_tag="PLT69_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ15090.1"
                     /translation="MKEKDYFLALYEVAKVINASLEVTKVLDEIAKSVTLAMDVKACS
                     IRLLDSRKKVLVMGASYGLSKGYIRKGPVLIKESGLDQQAISGKMIWIKNAQTDPSFQ
                     YGAGAKAEGIKSVIVAPLITKKSVIGVLRVYSDRIREFSAQETRFLEAVANLSALALE
                     NARFHQALQTNYDLLVAHKYRLDDN"
BASE COUNT          328 a          562 c          621 g          405 t
ORIGIN      
        1 tacaaactgc tcctcaaaat gttacgatct aaccccctaa agttgtcatg aaccataaat
       61 aaaggggtgc agtgaggtca aggttcaaga aggggtgtgc gtctcgagga tctcggcagc
      121 ccggggggct cacgttcgga cggccgggaa gagacggatg gacgcgtgta tcaggcccgg
      181 tcggtgtcgg ggagggactt cgagccgggc aagaggcaaa aggctcgggc ggaaagcatg
      241 ttcgggcaag acctcccggc aggcacatgc agggcgggga gtatcgaccc gccctgcatg
      301 gtcgtcgggt tcttgcctag tcgggtatgc ccttgagcgc gatgtagcgg gcgaggtccg
      361 caaccctgca cgagtagccc cactcgttgt cgtaccaggc cacgatcttc gcgagattgt
      421 cctgcatgac cctgacgtac tcgatgtcca cgatggagga cagctcgttc cccttgaagt
      481 ccacggacac cagcggtctc tcctcgcagc ccatgatccc cttgagcgtg gtcttggacg
      541 cccgcttgag gacggccctg aggtcgtcgg tggtggtgtt cttcctcagc tgtaccgtga
      601 ggtcgaccac agacaccgtg ggtgtcggca cgcgcagggc gaacccgtcg accttgccct
      661 tgaggtcggg gatgacgagg gacagggccc gtgcggcgcc cgtggaggtg gggatgatgc
      721 tcatggctgc ggcgcgtgcg cgcctgaggt ccttgtgggg catgtccagg atgcgctggt
      781 cgttcgtgta ggcgtggacc gtggtcatcc aacccttctc gatgccgaac gccttgtgta
      841 ccaccagggc aggcggcgcc aggcagttgg tggtgcacga ggcgttcgag atgacgtggt
      901 gcttcgtcgg gctgtacttg gtgtggttca cgcccatgac cacggtgatg tcctcctgct
      961 tggcgggcgc ggtgatgatg accttcttgg ccccgccgta gatgtgcgcc atggcgtcgg
     1021 tcttggacgt gaacagcccc gtggactcga tcacgatctg gacgccctcg tcgtcccacg
     1081 ggatgttcgc agggtcgcgc atggcgaagc tcttgatggt gttgccgttg atgacgaacg
     1141 agttgccctt gaccttcacc gaccccccga acgtgccgta gttcgtgtcg tacatgtaga
     1201 ggtgggcgtt ggtgttcacg tcgaagaggt cgttgatcgc cacaacctgc agcgtatcgc
     1261 cggcgcgttc catgatcgcc ttgagcacct ggcggcctat gcggccgaaa ccgtttatac
     1321 cgacacggat catatcatgc ctcctcacgc tcagttgtcg tcgaggcggt acttgtgcgc
     1381 cacgagcagg tcgtagttcg tctggagcgc ctggtggaac cgggcgttct ccagggccag
     1441 cgcgctcagg ttcgcgaccg cctccaggaa gcgcgtctcc tgggcagaga actcgcggat
     1501 cctgtctgag tagaccctga ggacgccgat caccgatttc ttggtgatga gcggagccac
     1561 gatcaccgac ttgatcccct cagccttcgc gccggcgccg tactggaagc tcgggtcggt
     1621 ctgggcgttc ttgatccaga tcatcttgcc cgagatggcc tgctggtcga gcccgctctc
     1681 ctttatcaac accgggccct tgcggatgta ccccttggag aggccgtatg aggcccccat
     1741 gacgaggacc tttttccggg aatcgaggag gcggatcgag caggccttga catccatcgc
     1801 gagcgtaacc gatttagcga tctcgtccag gaccttcgtt acctcgaggg aggcgttgat
     1861 caccttggca acctcgtaca acgccagaaa ataatccttt tctttcactc ttgcgc
//
LOCUS       DAOSCR010000547         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40206_length_1906_cov_3.759049, whole genome shotgun sequence.
ACCESSION   DAOSCR010000547 DAOSCR010000000
VERSION     DAOSCR010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40206_length_1906_cov_3.759049"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLT69_11410"
     CDS             <1..983
                     /locus_tag="PLT69_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOJ15091.1"
                     /translation="IHVVADPPAKGIEAMEAAPMLETASLAMKKVVEMVHLVAPSQAT
                     VLILGESGTGKEVVADMIHRLSPRSGRPMVKVNCAAIPETLIESELFGYEKGAFTGAV
                     GRKEGRFEAAHHSSIFLDEIGEVPPAVQVKLLRILQEGTFERLGSNKTQTVDVRIIAA
                     TNKDLAAMVREGSFREDLYWRLNVITIALPPLRDRKEDIPSLVQHFLTRFSHKNDKAV
                     RGIESQAMEALLGYGWPGNVRELENIIERSVVLDRDGVISLDDLPAQLVSTADEAAHT
                     VTIPLGTPLDEVERILMEETLKRTKGDKGLASKLLGISTRTLYRKMDRKD"
     gene            complement(1022..>1906)
                     /locus_tag="PLT69_11415"
     CDS             complement(1022..>1906)
                     /locus_tag="PLT69_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ15092.1"
                     /translation="VSESDATVLILGESGTGKELVARMIHHGSKRRLGKFVVMDCGAT
                     PSTLIEAELFGYGRGAFTGAVKDKRGIIEEAHGGTLFLDEIGNISPELQTRLLRVLEN
                     GEFRRLGELDQRRVDIRVIAATNADLKAKVDSGLFREDLFYRLRVITITIPPLRERRE
                     DIVGLAGVFLREFAEKTGKDVKGFSSKAMDLLLSHRWPGNVRELRNIVFSAVILTSGE
                     IITPEDLLPSGIGQDAAPSGPDDGLNPLEAQEKAMLIDALNRSNWVQKDAAKLLGISR
                     RVMHYKIRKFNITPEKKR"
BASE COUNT          337 a          614 c          620 g          335 t
ORIGIN      
        1 tcatccacgt cgtcgccgac ccgcccgcga agggcataga ggccatggag gcggcgccca
       61 tgctggagac cgcgagcctc gccatgaaga aggtcgtgga gatggtccac ctggtggccc
      121 cgagccaggc caccgtgctc atcctcggcg agagcgggac cggcaaggag gtcgtggcgg
      181 acatgatcca caggctctcc ccgaggtcgg gcaggcccat ggtcaaggtc aattgcgccg
      241 cgatccccga gaccctcatc gagtcggaac tcttcgggta cgagaaaggc gccttcaccg
      301 gtgcggtggg gaggaaagag ggcaggttcg aggccgccca ccactcgagc atcttcctgg
      361 atgagatcgg cgaggtgccg cccgcagtgc aggtcaagct cctgcgcatc ctccaggagg
      421 ggaccttcga gcgcttgggc agcaacaaga cccagacggt tgacgtcagg atcatcgcgg
      481 cgacgaacaa ggacctcgcg gccatggtca gggaggggag tttcagggaa gacctctact
      541 ggaggctgaa cgtcatcacc atagcgctgc cgccgctgcg cgaccggaag gaggacatac
      601 cgagcctcgt gcagcacttc ctgacgaggt tctcccacaa gaacgacaag gcggtgcgcg
      661 gcatcgagtc ccaggccatg gaggcgctgc tcggatacgg ctggcccgga aacgtccgcg
      721 agctcgagaa catcatcgag cggagcgtcg tgctcgaccg ggacggggtg atctccctcg
      781 acgacctgcc cgcgcagctc gtctccactg ccgacgaggc ggcccatacg gtgacgatcc
      841 ccttgggcac cccgttggac gaggtggaac gcatcctcat ggaagagacc ctcaagcgca
      901 ccaagggcga caaggggttg gcctcgaagc tcctcgggat atccacgagg acgctctacc
      961 ggaagatgga caggaaggat tgacgcccgg cgatcaggat gtccttgggc gaaagcgtcc
     1021 gttacctctt cttttccggg gtgatgttga acttcctgat cttgtagtgc atcacccggc
     1081 gcgagatccc gagcagcttg gcagcgtcct tctggaccca gttcgacctg ttcagcgcgt
     1141 cgatgagcat ggccttttcc tgtgcctcga gcgggttgag cccgtcgtcc ggcccgctcg
     1201 gcgccgcgtc ctgcccgatg ccggagggga ggaggtcctc gggggtgatg atctcaccgc
     1261 tggtgaggat cacggccgag aacacgatgt tcctgagttc acggacgttg ccgggccacc
     1321 ggtgggacag gaggaggtcc atggccttgc tgctgaaccc cttcacgtcc ttgccggtct
     1381 tctcggcgaa ctccctgagg aagacccctg ccaggcccac gatgtcttcc ctgcgttccc
     1441 gaaggggcgg gatggtgatg gtgatgaccc gcagccggta gaacaggtct tccctgaaca
     1501 gccccgagtc caccttggcc ttgaggtcgg cgttggtcgc ggctatcacc cggatgtcca
     1561 cccgtctctg atcgagctcg ccgagcctcc tgaactcgcc gttctcgagc accctgagga
     1621 gcctcgtctg gagttcagga gagatgttgc ctatctcgtc gaggaagagg gtgcctccgt
     1681 gcgcctcctc gatgatcccc ctcttgtcct tgaccgcgcc ggtgaaggcg cccctgccgt
     1741 aaccgaagag ctccgcctcg atcagggtgg aaggggttgc gccgcagtcc atgaccacga
     1801 acttgcccag cctcctcttg ctcccgtggt ggatcatgcg ggcgacgagc tccttgcccg
     1861 tgccgctctc gcccaggatg agcaccgtgg cgtccgactc gctcac
//
LOCUS       DAOSCR010000548         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40328_length_1902_cov_3.608013, whole genome shotgun sequence.
ACCESSION   DAOSCR010000548 DAOSCR010000000
VERSION     DAOSCR010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40328_length_1902_cov_3.608013"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLT69_11420"
     CDS             complement(<1..1317)
                     /locus_tag="PLT69_11420"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOJ15093.1"
                     /translation="MRKPLFLKIFAGYTLVTLTMSILMLVFSSSVIRTSYRDVTAKRL
                     EYEGIPLRALIAPLVQRRDLAALNRTVREFGSSTDARITIIDPQGVVLADSERDPSTM
                     DNHAHRPEVEQAMSGKTGRSIRYSATLKKDFLYLALPFDGARKVIGVLRLSIPLDHVD
                     TLMKSLLAHLALITAGILAVSLAMAAMISHVVSAPIKELARASGRLASGDLSIRVPPA
                     RNDEIRDLTEAFNHMAESLERSFREFSARKDELETIVSSVSEALFVLDAQGRLTVYND
                     AALRLAGVANAQGRYYWELFRSKDMGALLESTSPDQVSGEVELGGKTFLCSVGRVPSG
                     DERVVTLHDITDLKRMERYKKELVVNVSHELRTPLAAIKGFAETCLDEAGGEMREHLL
                     VILRHTNRLISIVNDLLVLSEMDERPMPKVQDTRIDDLAGNVTALYA"
     gene            complement(1314..1703)
                     /locus_tag="PLT69_11425"
     CDS             complement(1314..1703)
                     /locus_tag="PLT69_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15094.1"
                     /translation="MMHGEIHVVDDEPDVLRLAGIDLHNAGYGLDAFPGGAWFVEGLE
                     RSTPGPNILDATLMDPYGFETLPTLSPLRDGVCTRDRILGHLRGVGKTVPGMSVVDVH
                     FGETRQETGDRGPLTENVQSAGYKVQP"
BASE COUNT          310 a          585 c          625 g          382 t
ORIGIN      
        1 tgcgtacaag gcggtcacgt tcccggcgag gtcgtcgatc cgcgtatcct gcaccttcgg
       61 catgggcctc tcgtccattt ccgagagcac gagcaggtcg ttcacgatgg atatgagccg
      121 gttcgtgtgc ctgaggatga ccaggagatg ttcccgcatc tcgccgcccg cctcgtccag
      181 gcaggtctcc gcgaacccct tgatcgcggc caggggggtc ctgagctcgt gggagacgtt
      241 caccacgagt tccttcttgt agcgctccat gcgcttgagg tccgtgatgt cgtgcaaggt
      301 caccacgcgt tcgtccccgc tcgggacgcg gcccacgctg cacaggaagg tctttccccc
      361 gagttccacc tcgcccgaga cctggtccgg ggacgtggac tcgaggagcg cccccatgtc
      421 cttggacctg aagagctccc agtagtatct tccttgtgca ttcgcgacgc ccgcgagcct
      481 gagggcggcg tcgttgtaca cggtcagcct gccctgcgcg tcgagaacga agagggcctc
      541 gctcaccgag gacacgatgg tctcgagctc gtccttcctg gccgagaact ccctgaaaga
      601 acgctccagg ctctcggcca tgtggttgaa ggcctcggtg aggtcgcgga tctcgtcgtt
      661 gcgcgccggt ggtacccgga tggagaggtc tcccgaggca agcctgccgg atgcccttgc
      721 cagctccttg atgggcgccg agacgacgtg ggagatcatg gcggccatgg cgagcgagac
      781 ggcgaggatg cccgccgtga tgagagcgag gtgcgcaagg agggacttca tgagggtgtc
      841 cacgtgatcg agggggatgc tcaggcgaag gacgcctatg accttgcgtg cgccgtcgaa
      901 ggggagggcg aggtagagga aatccttctt gagggttgcg ctgtaacgga tgctccttcc
      961 ggtcttcccg ctcatggcct gctcgacctc cggccggtgc gcatggttgt ccatggtgga
     1021 ggggtctctc tcggaatccg caagtaccac cccttgcggg tcgatgatgg tgatccgggc
     1081 gtctgtcgag gacccgaact cccgcaccgt cctgttcagg gccgccaggt cgcggcgttg
     1141 tacgaggggt gctatgaggg cgcgcagggg gatgccttcg tactcgagcc tcttcgccgt
     1201 cacgtcgcgg taactcgtcc ggatgacact gctcgagaag acgagcatga ggatggacat
     1261 cgtcagggtg acgagggtat acccagcgaa gatcttgagg aacaagggtt tcctcatggt
     1321 tggaccttgt atcccgcgct ctgcacgttc tcggtgagag ggccccggtc accggtctct
     1381 tgccgggtct ccccgaagtg aacgtcgacg actgacatgc cgggcacggt cttcccgact
     1441 ccccggaggt gaccgaggat ccggtcgcgg gtgcataccc cgtcccggag gggggagagg
     1501 gtgggcaggg tctcgaaccc gtacgggtcc atgagcgtgg cgtcgaggat gttcggcccc
     1561 ggggtggaac gttccagccc ctcgacgaac cacgccccgc ccgggaacgc gtcgagaccg
     1621 tacccggcgt tgtggaggtc gatacccgcc agacggagga cgtcgggctc gtcgtcgacc
     1681 acgtggatct caccgtgcat cacctgggga cctcgccttg tccgagcatt ctcagctacg
     1741 gcctgtgtct tcgacctgga acaacccgtc cactcgccgg ccgatcatac tgtcctatac
     1801 cagatggctg cttgcctttc caaggcaata cgtcttttcg gcacgccctt taagggatgc
     1861 ggtttgcata acgattacga ttcttttggt tttctttcac ac
//
LOCUS       DAOSCR010000549         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40422_length_1899_cov_3.835683, whole genome shotgun sequence.
ACCESSION   DAOSCR010000549 DAOSCR010000000
VERSION     DAOSCR010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40422_length_1899_cov_3.835683"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..619)
                     /locus_tag="PLT69_11430"
     CDS             complement(236..619)
                     /locus_tag="PLT69_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15095.1"
                     /translation="MNKMRVLIMDDEDIVLDVVTSMLEFMGYEAEVSRTADEALEKYR
                     AAASEGKPFDAVILDLSIPGSRGGKEVIHDLAAIDPGVCALVSSGYSNDPVMTNYTEY
                     GFKGVVKKPFKMEDLRDALKKATGS"
     gene            718..1407
                     /locus_tag="PLT69_11435"
     CDS             718..1407
                     /locus_tag="PLT69_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOJ15096.1"
                     /translation="MKIAYVHKSSLIDYPGRISAVVFTIGCTFRCPYCHNPELVVHAS
                     GMTPIPDTEVLSFLEKRKGKLDAVVLSGGEPTMQPGLQGFMKKVKAMGFLVKLDTNGS
                     RPEVLERVIAGGLADYVAMDIKAPPRKYATASGTLVDISRVHRSIEIIMHGRVPYEFR
                     TTFVPSLHEPEDIVEIARMIEGANVYAIQRFVPSKHIDRAFMHLRSPEEEELVELAET
                     CGRWVGRCLVR"
     gene            complement(1417..>1899)
                     /locus_tag="PLT69_11440"
     CDS             complement(1417..>1899)
                     /locus_tag="PLT69_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15097.1"
                     /translation="VKGRGTTVTILLPLASAALSPETVTRVQDDGLQVAPAPECGQPT
                     VLIVEDDDTVRELVNEVLARHGYKTITARNGGDALQMARQYGGTIDLLITDMVMRRID
                     GKMLAKKMRSMWPGLRILFMSGFGSEIVDDDDLKDTAFLPKPFLPADLISKVENVMRP
                     "
BASE COUNT          337 a          587 c          601 g          374 t
ORIGIN      
        1 gggaccccat aggggtatct tcgtgatcga cagcgtaaac gacgaggatc cccgcatgag
       61 ggaaatacgg gtcgccttgt cgttcggggt cgcccaaggg gagatgcccc ctaccggaag
      121 gaatatggcc gtcttgaggc accgttaccc gcttcactcg tggtacgccc gggtggttcg
      181 ccccgagggt gtccgggcga cggactgggg tgccgagcgc accggtgcag ggcgctcagg
      241 agcctgtggc cttcttcagg gcgtctctca agtcttccat cttgaagggc ttcttgacca
      301 cacccttgaa gccgtattcg gtgtaattgg tcatgacagg gtcgtttgaa tacccgctgg
      361 acacgagggc gcacacgccg gggtcaatgg cggcgaggtc atgtatgacc tccttgcctc
      421 cccgggaccc gggtatggaa agatcgagga tcaccgcgtc gaagggtttc ccctcggagg
      481 ctgcagcgcg gtacttctcg agcgcctcat cggcggttct cgagacctcg gcctcgtacc
      541 ccatgaactc gagcatgctc gtgacgacgt cgaggacgat gtcctcgtcg tccatgatca
      601 ggacccgcat cttgttcatg cctgcatcct tgtgcgagtg atgaacacat tcataacaca
      661 cggttcttat aaatcaagcc gaacgtgttc gtccggcgct taccggtgcc tggagacatg
      721 aagatcgcat acgtccacaa gagttccctc atcgattacc cgggcaggat ctccgccgtg
      781 gtgttcacca tcggctgcac cttcaggtgc ccctattgcc acaacccgga gctggtcgtg
      841 catgcctcgg gcatgacgcc cataccggac acagaggtcc tctcgttcct cgagaagagg
      901 aaaggaaagc tcgacgccgt ggtgctctcc gggggggagc ccaccatgca gccggggctt
      961 caaggtttca tgaagaaggt caaggcgatg ggatttctcg tcaagctgga caccaacggt
     1021 tcgaggcccg aggtgctcga gcgtgtcatt gcggggggcc tggcggatta cgtggccatg
     1081 gacatcaagg ccccgccgcg caagtatgcg accgcttccg gcaccctcgt cgacatctcg
     1141 cgggtgcaca ggagtatcga gatcatcatg cacggccggg tcccctacga gttcaggacc
     1201 accttcgtgc cctccctcca cgaaccggag gacatcgtcg agatcgcgag gatgatcgag
     1261 ggggcaaacg tgtacgccat ccagaggttc gtcccctcca agcacatcga ccgtgcgttc
     1321 atgcacctgc ggtcccccga ggaggaggag ctggtggagc tggccgagac ctgcggccgc
     1381 tgggtcggcc gttgcctcgt tcggtaggat cacccctcac gggcgcatga cgttctcgac
     1441 cttggagatg aggtctgcag ggagaaacgg cttggggagg aaggcggtgt ccttgaggtc
     1501 gtcgtcgtcg acgatctcgc tgccgaaccc cgacatgaag agtatcctca ggccgggcca
     1561 catggagcgc atcttcttcg cgagcatctt cccgtcgatc ctgcgcatga ccatgtccgt
     1621 gatgaggagg tcgatggtgc cgccgtactg cctcgccatc tggagcgcgt cgccgccgtt
     1681 gcgagcggtg atggtcttgt agccgtgcct cgccagcacc tcgttcacga gttccctcac
     1741 cgtgtcgtcg tcctccacga tgaggaccgt gggttggccg cactcgggcg ccggcgccac
     1801 ctgaagcccg tcatcttgca ccctggtgac ggtctccggc gacaaggcgg ccgaggccag
     1861 tggcaggagg atcgtcacgg tggtgcccct gcccttgac
//
LOCUS       DAOSCR010000550         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40822_length_1887_cov_4.413755, whole genome shotgun sequence.
ACCESSION   DAOSCR010000550 DAOSCR010000000
VERSION     DAOSCR010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40822_length_1887_cov_4.413755"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..603
                     /locus_tag="PLT69_11445"
     CDS             331..603
                     /locus_tag="PLT69_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15098.1"
                     /translation="MKRTIQTLAVAAFVVLVTSTFAFAAAATAGGAFPFFHLGCLVVG
                     GLTIVSLKHRYPRIYLSEAMGSFALYAVLVALFTAPVIEAVKTLVS"
     gene            752..1012
                     /locus_tag="PLT69_11450"
     CDS             752..1012
                     /locus_tag="PLT69_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15099.1"
                     /translation="MRRDDRKRNRIENLTELLVLSAWGAVIAIASILFVLVGVRLDTA
                     FETGPLFMIGLLVLAVFLLVARLYVEFKKTSNRMDTMGRRHA"
     gene            complement(1209..>1887)
                     /locus_tag="PLT69_11455"
     CDS             complement(1209..>1887)
                     /locus_tag="PLT69_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOJ15100.1"
                     /translation="VVKLVGPEQGAGLTGLVGGLVSSTAVTLGLSQKSRSDPGLSRAL
                     ALGVMSSWTVMYLRVMVIAVVLNMSLFTALWKAMLGLFAVSCAYCLYLYTSHRGESGE
                     EARTFRNPFELLPAITFGVLYAVILLGVNTASIFFGDTMVYVSSLISGLLDVDAITLS
                     MSRLSGMQGGLHISVASRAVVIACAANTFLKGAMVLAIASSGVRRLVIPGTCVILAAQ
                     VLAVVLI"
BASE COUNT          368 a          608 c          601 g          310 t
ORIGIN      
        1 gcggggggag gatttgaacc tccgaccttt gggttatgag cccaacgagc tacctgactg
       61 ctccaccccg cgtcacgaag gcctttctac aatcatgggt agcctttgtc aacctccttc
      121 gacatttcgt ccaaggggat tgaacgtatc gtgcgaaaca ggggcggccg ggagcaccgc
      181 ggagacccgt ccccgaggcg gcggcccttc ctatcgccag ttatttcatt atgttacgca
      241 cctcgtgccg agggtgacgg cctctggcat gcactatgct tcatactgga caggatcaca
      301 aaaaagacaa gaacacagga gggcacgaag atgaaaagga cgattcaaac tctcgcggtt
      361 gcggcattcg tggtcctcgt cacctccacc ttcgcgttcg cggcggctgc gacggcaggc
      421 ggcgcgttcc ccttcttcca tctcggctgc ctggtcgtcg gagggctcac catcgtgtcc
      481 ctcaagcacc gctaccccag gatctacctc tccgaggcca tgggttcgtt cgcgctctac
      541 gcggtgctgg tggcgctgtt cacggcgccc gtgatcgagg ccgtcaagac gctggtgagc
      601 tgacgagggg cgcggcccag gcggctccgg gcacgtgagt gcgccgcgtg gggccgaagg
      661 gacgaggaag acggttcaag ggtacatgga tcgaggccac ccgggacacc ggcagcccgt
      721 gtcccgggcc acgggaaagg agggaacgga catgagacgg gatgacagga aaaggaacag
      781 gatcgagaac ctcaccgagc tgctcgtgct gagcgcatgg ggtgcagtca tcgccatcgc
      841 ctccatcctc ttcgtgctcg tgggcgtgag gcttgacacc gcattcgaga ccgggccgct
      901 cttcatgatc ggtctcctcg tcctggcggt cttcctcctg gtggcaaggc tctacgtgga
      961 gttcaagaag accagcaaca ggatggacac catgggcagg cggcacgcct gagaaagcgg
     1021 gcgccgaagg cgttcgcgtg ccagaggggc cccgtaaggg aaagacagca gcatcaagcg
     1081 tgagggggac gccgggaggc gtccccctca cgctttcccg ccctgtgacg atcgtagtct
     1141 gcgcctgaac cgctcggctc cgcccggtgc gttcttcatg acttcccgcc acggcctggg
     1201 ccgggcgatc agatcagcac cacagccagg acctgcgccg cgaggatgac gcaggttccc
     1261 gggatgacga gcctcctgac cccggatgaa gctatcgcca agaccatggc ccccttgagg
     1321 aacgtgttgg ccgcgcaggc gatcaccacc gcgcgggaag ccacggatat gtggagcccg
     1381 ccctgcatgc cgctcagcct ggacatggac agtgtgatgg catccacgtc cagaaggccc
     1441 gagatcaggc tggagacata taccatcgta tccccgaaga aaatcgacgc cgtgttgacc
     1501 ccgagcagga tcaccgcgta gagcacgccg aaggtgatgg cgggaaggag ctcgaagggg
     1561 ttcctgaagg tcctcgcctc ctcgccggac tcacccctgt ggctcgtgta caggtagagg
     1621 cagtaggcgc aggagaccgc gaacagcccg agcatggcct tccacagcgc ggtgaacaac
     1681 gacatgttca acaccaccgc gatgaccatg accctcaggt acatgaccgt ccaggacgac
     1741 atgacgccca aggcgagcgc ccgcgagagg ccggggtcgc tcctgctctt ctgcgagagg
     1801 ccgagcgtca ccgcggtgct gctgaccagg cctcccacca gcccggtcag ccccgccccc
     1861 tgctccgggc ccacgagctt gacgacc
//
LOCUS       DAOSCR010000551         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40827_length_1887_cov_3.553493, whole genome shotgun sequence.
ACCESSION   DAOSCR010000551 DAOSCR010000000
VERSION     DAOSCR010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40827_length_1887_cov_3.553493"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLT69_11460"
     CDS             complement(<1..616)
                     /locus_tag="PLT69_11460"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HOJ15101.1"
                     /translation="MDDRRLFASVVCRVTYSIVDGTLKAAPEQVWRVSAEPWEGPYGP
                     MDSDEVFYTGGVDVFAFGSARPHRPETGVMEVSVEVGSSFTYSIVVFGRRVWRKVKGS
                     LVASPPAPFSEIPLTLEHAYGGKDTWDGLDVPYPANPLGKGFYVDEKNAEGGELPNIE
                     EKGRLISRWDDRPEPVGVACCGMAFGPRLEQSLVFDEKTGALTEI"
     gene            complement(676..>1887)
                     /locus_tag="PLT69_11465"
     CDS             complement(676..>1887)
                     /locus_tag="PLT69_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ15102.1"
                     /translation="TEKRLAQLDVKWKAELEAARKVIECRSAIREIHDRDPKDKELAK
                     RQKELKELEKDLAKAQGGDPLVFIEVTGQTVSEVISGWTGIPVGRMMADEIQTVLDME
                     NLLKKRIVGQDHALRAVAQMVQTAHAGMEDPSKPTAVFMFVGTSGVGKTETALAISEL
                     LYGGEENVITINMSEYQEAHTVSSLKGSPPGYVGYGEGGVLTEAVRRRPYSVVLLDEV
                     EKAHPDVLELFYQVFDKGRMEDGEGREIDFRNTLIILTSNVGTDLIMKACADEETRPS
                     PEALVELVRPELLKHFKPAFLGRMKIVPFYPITDDIMRLIVSLKLERIKTRMMENRKV
                     LFEYAEELVDAIAGRCTEVDSGARNVDHILTNTLLPDMSREILARMARGESIERVRVG
                     LEGSGFVYEIV"
BASE COUNT          294 a          644 c          592 g          357 t
ORIGIN      
        1 tgatctcggt gagggcgccg gtcttctcgt cgaagacaag ggactgctcg agcctggggc
       61 cgaacgccat gccgcagcat gcgacgccca cgggctccgg cctgtcgtcc cacctggaga
      121 tgagacgccc cttctcctcg atgttcggaa gctcgccgcc ttccgcgttc ttctcgtcga
      181 catagaaccc cttgcccaag gggttggcgg ggtaagggac gtcgaggccg tcccaggtgt
      241 ccttgccccc gtaggcgtgt tcgagggtga gcgggatctc ggaaaacggg gcgggcgggg
      301 aggcgacgag ggaccccttg accttcctcc acacgcggcg tccgaagacc acgatggaat
      361 acgtgaacga ggaccccacc tcgacggaca cctccatgac ccccgtctcc gggcggtgag
      421 gcctggcgct gccgaacgcg aagacgtcga ccccgcccgt gtagaacacc tcgtccgagt
      481 ccatgggccc gtacgggccc tcccacggct ccgccgacac ccgccacacc tgctcggggg
      541 ccgccttcag cgtgccgtcg acgatcgagt aggtcacccg gcagaccacg gaggcgaaga
      601 ggcgcctgtc atccaccacc cccctgaaga gcatcgccgg gaaaggggtc gagttcgtaa
      661 gaagcatgtc tcgggctaaa cgatctcgta cacgaagccc gacccctcga ggccgaccct
      721 cacgcgctcg atgctctcac ccctcgccat gcgcgcgagg atctcccggg acatgtcggg
      781 caggagcgtg ttggtgagga tgtggtccac gttgcgtgcg ccgctgtcca cctcggtgca
      841 gcggcccgcg atcgcgtcca ccagctcctc agcgtactcg aagagcacct tgcggttttc
      901 catcatgcgc gtcttgatgc gttcgagctt gagggacacg atgagcctca tgatgtcgtc
      961 ggtgatgggg tagaacggca cgatcttcat ccgtcccagg aacgccggct tgaagtgctt
     1021 gaggagctcg ggcctgacga gctccacgag cgcctcgggg gaaggcctcg tctcctcgtc
     1081 ggcgcacgcc ttcatgatga ggtcggtgcc cacgttcgag gtcaggatga tgagggtgtt
     1141 cctgaagtcg atctcgcgtc cctcgccgtc ctccatcctc cccttgtcga agacctggta
     1201 gaagagctcg aggacgtcgg ggtgggcctt ctccacctcg tcgaggagaa ccacgctgta
     1261 aggcctcctc ctgacggcct cggtgaggac gccgccctcg ccgtacccca cgtagccggg
     1321 gggcgacccc ttcaggctcg agaccgtgtg ggcctcctgg tactcggaca tgttgatggt
     1381 gatcacgttc tcctccccgc cgtagaggag ctcggagatc gcgagggccg tttccgtctt
     1441 ccccacgccg ctcgtcccca cgaacatgaa gaccgccgtg ggcttggagg ggtcctccat
     1501 cccggcgtga gcggtctgga ccatctgggc gacggccctc agggcatggt cctggcccac
     1561 gatgcgtttc ttgaggaggt tctccatgtc gagcacggtc tgtatctcgt cagccatcat
     1621 cctgcccacc gggatccccg tccagccgga gatcacctcg gagacggtct ggccggtgac
     1681 ctcgatgaag acgaggggat cgccgccctg ggccttcgcg aggtccttct cgagctcctt
     1741 gagctccttc tgccttttcg ccagttcctt gtccttcggg tcgcgatcgt gtatctcgcg
     1801 gatggcgctg cggcactcga tgacctttcg ggccgcctcc agctccgcct tccacttcac
     1861 gtccaactgc gcgaggcgtt tctcggt
//
LOCUS       DAOSCR010000552         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40922_length_1884_cov_3.762712, whole genome shotgun sequence.
ACCESSION   DAOSCR010000552 DAOSCR010000000
VERSION     DAOSCR010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40922_length_1884_cov_3.762712"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLT69_11470"
     CDS             <1..466
                     /locus_tag="PLT69_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15103.1"
                     /translation="QVKGSAEGHAVYKKISNASQRFLETHVEFLGSVFFDDKLKRAVR
                     DQVPVVARHPTSDVARCYRVIALTLLGQEQVGVDLEKFWSRLVSLVVKTRRPRTMRAV
                     SFNGEAAQRSMEKTFLDILDEQRKTRMLLERLISTIEGGVSHASNARKGSKA"
     gene            463..1050
                     /locus_tag="PLT69_11475"
     CDS             463..1050
                     /locus_tag="PLT69_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOJ15104.1"
                     /translation="MIARLETPFGCLWTAGSADAVEKVSWTPLDGPHHKGELDWILDP
                     LRAYFDGALEALPGRILFLAPGVLWVKNHVNTRPHTRAQEILDLIFAIPYGKRATYGE
                     IAASVGYKKGARAIGAVCRSNPLPVLIPCHRVVGARSLGGYTPDVTIKEGLLCLESKV
                     LASRRPTGSTGSLPSNIPLAQGGGNGWVDSPDGVS"
     gene            1124..>1884
                     /gene="moaA"
                     /locus_tag="PLT69_11480"
     CDS             1124..>1884
                     /gene="moaA"
                     /locus_tag="PLT69_11480"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOJ15105.1"
                     /translation="MDLHGRAIRYLRLSVTDRCNLRCRYCMPPVGTEWVPHEDVLSYE
                     EMERILGVCSRLGVSKVRITGGEPLLRKGIEGFIARISAMGAVKDLSLTTNGVLLAGK
                     ARELRRSGLHRVNVSLDTLDRKKFAHITRVDAFDDVIRGICAAYEHGLRPVKVNVVAI
                     KGFNDDEVAEFAGLTVRLPVEVRFIELMPLGCVSRFPELVHVEAGEILARIEEAYGGL
                     EELEKGLGPATVYKLPGAHGRIGIIGALSGHAFCAS"
BASE COUNT          357 a          567 c          640 g          320 t
ORIGIN      
        1 ccaggtcaag ggaagcgccg aggggcatgc cgtctacaag aagatctcca atgcctcgca
       61 gcggttcctc gagacgcacg tggaattctt gggcagcgtc tttttcgacg acaagctcaa
      121 gcgcgcggtt cgcgaccagg tccccgtggt cgcgaggcat cccacctcgg acgtggcgag
      181 gtgctaccgg gtgatagccc tcaccctcct cggccaggaa caggtgggag tggatctcga
      241 aaagttctgg tccaggctcg tgagcctggt ggtgaagacg aggcggccga ggaccatgag
      301 ggccgtgtcc ttcaacggtg aagccgccca gaggtccatg gaaaagacct tcctggacat
      361 actcgatgag caacgcaaga cccgcatgct tcttgagcgc ctgatatcga ccatagaggg
      421 cggggtttcc catgcatcga acgccaggaa ggggtccaag gcgtgatcgc gaggctcgag
      481 acccccttcg gctgtctctg gacggcggga tcggcggacg cggtggagaa ggtgtcgtgg
      541 acgcccctcg acggacccca tcacaagggt gagcttgact ggatcctcga ccccttgcga
      601 gcttacttcg acggggccct cgaggcgctc cccgggagga tcttgttttt agccccaggg
      661 gtcctctggg tgaaaaacca cgtgaacacg aggccgcata ccagggccca agagatcctg
      721 gacctcatct ttgccattcc gtacggaaag agggcgacct acggcgagat agccgcatcc
      781 gtcgggtaca agaaaggggc cagggccatc ggtgcagtat gcagatcgaa ccccctcccg
      841 gttctcatcc cctgtcaccg ggtcgtgggt gctcgcagcc tgggaggata cacgcccgac
      901 gtgacgatca aggaagggct cctgtgcctg gaaagcaagg tcctcgcctc caggagaccc
      961 accgggtcga ccgggagcct gccctcgaac attccgcttg cccagggggg tggcaacggg
     1021 tgggttgact cgccggacgg ggtgtcatag gctgggcgag atggaaaagc cgtgcccgcg
     1081 atcttgtcgg cccgaagatc cagagccgaa ggccaggggc ctcgtggatc tgcacgggag
     1141 ggcgatcagg tacctgaggc tctcggtcac cgataggtgc aacctgagat gccggtactg
     1201 catgcccccc gtgggcaccg agtgggtccc ccacgaggac gtactctcct acgaggagat
     1261 ggagcgcata ctcggtgtct gttcgaggct gggcgtctcc aaggtgagga tcaccggcgg
     1321 ggaacccctg ctgaggaagg ggatcgaggg cttcatagcg cgtataagcg ccatgggtgc
     1381 cgtcaaagac ctttcgctca ccaccaacgg tgttctcctg gccgggaagg cccgggagct
     1441 caggcgctcg gggctgcaca gggtgaacgt cagcctcgac accctggaca ggaagaagtt
     1501 cgcccacatc acgcgagtgg acgccttcga cgacgtgatc cgggggatct gcgccgcgta
     1561 cgagcacggc ctgaggcctg tcaaggtcaa cgtggtcgcg atcaagggct tcaacgacga
     1621 cgaggtggcc gagttcgccg gcttgaccgt gaggctcccc gtcgaggtcc gcttcatcga
     1681 gctcatgccc cttgggtgcg tctcgcggtt ccccgagctc gtgcacgtgg aggcgggcga
     1741 gatccttgcg cgcatcgagg aggcctacgg cgggctcgaa gaactcgaaa aggggctcgg
     1801 tccggcgacc gtctacaagc tcccgggggc gcacggcagg atagggatca tcggcgcctt
     1861 gagcgggcat gcattctgtg cctc
//
LOCUS       DAOSCR010000553         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_40989_length_1882_cov_2.870826, whole genome shotgun sequence.
ACCESSION   DAOSCR010000553 DAOSCR010000000
VERSION     DAOSCR010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40989_length_1882_cov_2.870826"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..668)
                     /locus_tag="PLT69_11485"
     CDS             complement(87..668)
                     /locus_tag="PLT69_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOJ15106.1"
                     /translation="MKMREVKQMLFETPGKHNTDHCIDVVRRYVTERGQTHVVVASTT
                     GRTALAFCRALKNSPARLVVVTHSVGFKEPNKDEFEPEARRILSQEGIPVHTGTILTH
                     SLEKSLMDDFHGTYPGYIIATTLRRFGEGTKVAVEVIMEACDAGLIPEDEEVLGVGGT
                     GYGADTVLLVRSKPSKRFGKLEVLEILARPRGR"
     gene            complement(772..>1882)
                     /locus_tag="PLT69_11490"
     CDS             complement(772..>1882)
                     /locus_tag="PLT69_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15107.1"
                     /translation="LLARAGLSVAMFDKCVFPRQKLCGGLLPDKTVRVVERLFGIHRS
                     SLQDKGVIVHTSNTYRIYSGGRLLVEGRSPRPFTLVDRAAYDSFLLELARTQGVEIHE
                     GQKVERVDPYGTFIETASGARHYGRFIIGADGALGVTARCAANALARERDRRILAGSL
                     DVTLPVEKLGRPVFCPEILLGYVDWGYAWAFPSGRSVTLGLGGLQRKNKKTFKELLND
                     ICEDIGVKRLGETEPKGHPIPFGRPGDRLAVGSLVLVGDAAGLADPITGEGVYSAHLS
                     SEAASAAVIRALRYGGSLGAIYRRLIDRRLLGLLEGAARLQPLVYTALEVLPRSVSGM
                     LVRRFHTRALRVINGDKGYPFSRTGHPDAALPSFV"
BASE COUNT          351 a          599 c          559 g          373 t
ORIGIN      
        1 cccgccgacc accgtgcatg ctcgatggcg catccgaagg cgtcacgtca tgtgggttta
       61 cggttcggaa acatcgatgg tccatctcac cgacccctgg gtcgagcgag tatctcgagc
      121 acctcgagtt tcccgaaacg tttcgagggc ttcgatcgta cgagcaggac cgtgtccgcg
      181 ccgtagcccg tcccgcccac accgagaacc tcttcgtctt ccgggatgag ccctgcgtcg
      241 catgcctcca tgatgacctc caccgcgacc ttcgtgccct cgccgaatcg cctcaaggtg
      301 gtcgctatga tgtatcccgg gtacgtgccg tgaaagtcgt ccatgagaga tttctcgaga
      361 gaatgggtga ggatggtgcc cgtgtgcacg ggtatgccct cctgtgacag gatgcgcctc
      421 gcctcgggct cgaactcatc cttgttgggt tccttgaacc ctacgctgtg ggtgaccacc
      481 acgagacggg caggcgaatt cttgagtgcg cggcagaagg cgagggctgt cctcccggtg
      541 gtcgacgcca cgaccacgtg ggtctggccg cgctcggtga cgtaccgcct cacgacatcg
      601 atacagtgat cggtgttgtg cttcccgggc gtctcgaaga gcatctgttt gacctctctc
      661 atcttcatga ccagtaacct cctcgggtaa ccttctcatg catgtgcgcc ggcgcgcagg
      721 tcgtgtctct tccatgcagg aaactgttgc ctcatgcaag atcaaggggt gtcacacgaa
      781 tgatggcaga gcggcatctg gatgcccagt ccttgagaag ggataacctt tgtccccgtt
      841 gatcacccgc aacgccctcg tgtggaagcg cctcacgagc atgccggaga cggacctggg
      901 gagcacctcg agggctgtgt agacaagggg ttgcaacctc gcggcacctt cgagcaggcc
      961 cagcaggcgc ctgtcaatca acctgcggta gatcgcccca agagacccgc cgtacctgag
     1021 ggcacggatg acggccgccg atgccgcttc ggaactgagg tgggcggagt acaccccttc
     1081 gcctgttatg gggtctgcaa ggcctgctgc gtcgccgacc aggaccagag accccacggc
     1141 cagacgatct cccggccttc cgaagggaat ggggtggcct ttgggctcgg tctccccaag
     1201 tctcttcacg ccgatgtcct cgcagatgtc gttcaacaac tccttgaatg tcttcttgtt
     1261 cttcctctgc aaaccaccca ggcccaaggt gacggacctc ccggagggga aggcccatgc
     1321 atacccccaa tcgacgtaac ccagcaagat ctcggggcag aagacgggtc ttcccagctt
     1381 ctcgacaggc agggtcacat cgagcgaacc cgcgaggatc cgtcggtctc tttcccgggc
     1441 cagggcgttg gctgcgcatc gtgcagtcac acccagggcg ccgtccgcgc cgatgatgaa
     1501 ccggccgtaa tgccgagcgc ctgaggctgt ctcgatgaac gtcccgtatg gatcgaccct
     1561 ctctaccttt tggccctcgt ggatctcgac accttgtgtt ctcgccagct cgaggaggaa
     1621 cgaatcatag gccgccctat cgacaagggt gaaaggacgg ggtgaacgac cttccacgag
     1681 gagcctgccc ccggaataga tgcggtaggt gttcgacgtg tgcacgatca cccccttgtc
     1741 ttgaagggag gacctgtgga tcccgaacag cctctcaacg accctgacgg tcttgtccgg
     1801 caggagcccg ccgcagagct tctgtcttgg gaacacgcac ttgtcgaaca tggccactga
     1861 aaggccggct ctagcaagca gg
//
LOCUS       DAOSCR010000554         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_41161_length_1876_cov_3.108731, whole genome shotgun sequence.
ACCESSION   DAOSCR010000554 DAOSCR010000000
VERSION     DAOSCR010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41161_length_1876_cov_3.108731"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLT69_11495"
     CDS             <1..841
                     /locus_tag="PLT69_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOJ15108.1"
                     /translation="SIAMGIVILMVGMYTTNPLNFSVFPTLLLIVTLYRLALNIASTR
                     IILLHGYEGEMAAGKVIHTFGQFVVGGSYVVGAVVFCIFVLINFVVITKGATRIGEVT
                     ARFTLDAMPGKQMAIDADLNAGMITEEEARQRRERIAREAEFYGAMDGATKFVRGDAI
                     AGIVIVMINIVGGIIIGVLQNQMTFTDALRTYTTLTIGDGLVAQIPALIISTAAGIIV
                     TQSAADKSVGKQLSDQLSFQPMALLTAAGIIFFFGLLPGMPHVAFLTMATVMGAVGWR
                     ASG"
     gene            838..>1876
                     /locus_tag="PLT69_11500"
     CDS             838..>1876
                     /locus_tag="PLT69_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOJ15109.1"
                     /translation="MREAAKEEEVKPVEAAPGDVSAESLLPMDILEFEVGYGLIPLVD
                     TAQGGDLLSRIKGMRRTVASELGIVVPPIHVRDNLELKPNEYRLLLKGIEVARGEAMA
                     DRLMVLNPDRVEIDLPGIPVKEPAFGLPGLWIKKADSERAQMAGLTIVDAATVVATHL
                     TEAVKANAHDLLGRQELQSILDAVSRTHPKVVDDLVPQVLPAGVVLRVLRNLLKEKVP
                     VKNIITILETLADFGHMTKDPDILTEYVRQSLMRIVSAPYVSGGTLSVLALDPAVDGM
                     ISNAIQHTDHGSYLALDPEKSQRILVALNREVTNVSKKGVVPVLLTSPISRMYVKRLT
                     ERYIPDLVVLSH"
BASE COUNT          355 a          615 c          600 g          306 t
ORIGIN      
        1 cagcatcgcc atggggatcg tgatcctcat ggtgggcatg tacaccacca acccgctgaa
       61 cttctcggtg ttccccacgc tcctgctcat cgtgaccctg taccggctcg cgctcaacat
      121 agcctccacg cgcatcatcc tcctccacgg ctacgagggg gaaatggcgg cggggaaggt
      181 gatccacacc ttcggccagt tcgtggtcgg cggcagctac gtcgtcggcg cggtggtgtt
      241 ctgcatcttc gtgctcatca acttcgtggt catcaccaag ggcgccaccc gcataggcga
      301 ggtgaccgcc cggttcaccc tcgacgccat gcccggcaag cagatggcca tagacgcgga
      361 cctgaacgcc ggcatgatca ccgaggagga ggccaggcag cgcagggagc gcatcgcgcg
      421 ggaggcggag ttctacgggg ccatggacgg cgccaccaag ttcgtgcggg gggacgccat
      481 agccggcatc gtgatcgtga tgatcaacat cgtgggcggc atcatcatcg gcgtgctcca
      541 gaaccagatg acgttcaccg acgcgctgag gacctacacc accctcacca tcggcgacgg
      601 gctggtcgcc cagatccccg ccctcatcat ctccaccgcc gcgggcatca tcgtgaccca
      661 gtcggcagcg gacaagtcgg tgggcaagca gctctccgac cagctctcgt tccagcccat
      721 ggccctgctc acggccgcgg gcatcatctt cttcttcggc ctgttgcccg gcatgcccca
      781 cgtggcgttc ctcaccatgg ccaccgtcat gggcgccgtg gggtggagag cctcagggtg
      841 agggaggccg cgaaggagga ggaggtcaag ccggtcgagg cggcgccggg cgacgtgtct
      901 gcggagagcc tcctgcccat ggacatcctc gagttcgagg tgggctacgg gctcataccc
      961 ctcgtcgaca cggcgcaggg aggcgacctc ctgagcagga tcaagggcat gcgccggacc
     1021 gtggcctctg agctcggcat cgtcgtaccg cccatccacg tgagggacaa cctcgagctc
     1081 aagcccaacg agtaccgcct cctcctcaag gggatagagg tcgcacgggg tgaggccatg
     1141 gcggacaggc tcatggtcct caacccggac agggtggaga tcgacctgcc cggcataccc
     1201 gtgaaggaac ccgcattcgg cctgcccggc ctgtggataa agaaggcgga cagcgagcgg
     1261 gcgcagatgg ccggcctcac catagtggat gcggcgaccg tggtggccac ccacctcacg
     1321 gaggcggtca aggccaacgc ccacgacctg ctcggaaggc aggagctcca gtccatcctg
     1381 gacgcggtct cgaggacgca cccgaaggtc gtggacgacc tcgtcccgca ggtcctgccc
     1441 gccggggtgg tgctcagggt cctcaggaac ctcctcaagg agaaggtccc ggtcaagaac
     1501 atcatcacca tcctggagac cctcgccgat ttcgggcaca tgacgaagga ccccgatatt
     1561 ttgacggagt acgtcagaca atccctcatg cgcatcgtga gcgccccgta cgtgagcggg
     1621 gggaccttga gcgtactggc tctggatccc gctgtggacg ggatgatctc caacgcgatc
     1681 cagcacacgg accatggttc gtatcttgca ttggacccgg agaagtcgca gcggatcctg
     1741 gtggctctga acagggaggt gaccaacgtg agcaagaaag gcgtcgtacc ggttctgctg
     1801 acctcgccca tcagcaggat gtacgtgaag aggctcaccg agaggtacat accggacctc
     1861 gtcgtgctct cgcaca
//
LOCUS       DAOSCR010000555         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_41866_length_1855_cov_3.693889, whole genome shotgun sequence.
ACCESSION   DAOSCR010000555 DAOSCR010000000
VERSION     DAOSCR010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41866_length_1855_cov_3.693889"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLT69_11505"
     CDS             complement(<1..410)
                     /locus_tag="PLT69_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017049539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ15110.1"
                     /translation="MKRGSRDNAKVSAASLSVASNTMLIVLKTASGIAMGSISMISEA
                     AHSGVDLVAAVIAFFSVRTSGKPADKDHPFGHRKIENLSGAVEALLIFLAAGWIVIEA
                     VGRIMHPSPVTAVGWGVIVMLASCLVNLAVSHRL"
     gene            879..>1855
                     /locus_tag="PLT69_11510"
     CDS             879..>1855
                     /locus_tag="PLT69_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15111.1"
                     /translation="MKRSVKTIQYVLIAVLLAAGAAFTFVYLEWEKPVIEVDRPFDTI
                     GSARSVAVVMKDAKSGIRSYEVALVQGGKRFIVEGADLPEKGFHDKRLQLEISPGRLG
                     VKDGEAVFSVRVTDFSPLRNTAVYEAKVKVDQVPPRIAVLSRAHNVNAGGTCLVVYRV
                     TKEVAASGVKCQDSFFPGYEVRGQKGSYFIAYFAVPRDMTPSTPVAVTAVDKGGNSAV
                     AAVPVYLRKTAPFRSDTVAVSDSFLQQKAAEFQQADSRLANKSPQEIFSFVNSTVRQE
                     NDAKIRSICARSAPERIWDGDAFLRMKNAAPKALFGDERTYVYRGVPLGS"
BASE COUNT          357 a          560 c          640 g          298 t
ORIGIN      
        1 aagagcctgt gagagacggc caggttgacc aggcaggagg ccagcatcac gattacgccc
       61 cacccgacgg ccgtcaccgg ggagggatgc atgatcctcc cgacggcctc gatgacgatc
      121 cagcccgccg ccaagaagat gaggagcgct tccacggccc cggacaggtt ctctatcttc
      181 cggtggccga aggggtggtc cttgtccgcc ggtttgcccg aggtcctcac cgagaagaag
      241 gctatcacgg ccgcaacgag gtccacgccc gagtgggcgg cctcggagat catggagatg
      301 gatcccatgg cgatgccgga tgccgtcttc aggacgatga gcatcgtgtt ggaagccacg
      361 gacaggcttg ccgcggatac cttggcgttg tcgcgcgatc ctcttttcat ggggtgacct
      421 tcgagcggtt ctgtggaagg tataccagag agctcccggc agcgccagca tccatcttca
      481 ggtggagacc cgccgggggg acgaggcccg cgaaagcaga cggtgtctga ccgaagaaga
      541 cggcccgtga ccgcgggggt cgctcgggtg gaacacccgg gtatgcgtgc atgaaaaagg
      601 ggtggctcga tccctgcgcg gcccgacacg agacggtcgg caaagtggat ggtatagggc
      661 gggctcgctc gggtgactcc ctgttgccca cccgagcctt caggcaggac ccgtgtgggt
      721 gaccaggagg gcctgcccca caggcgggac cgttctcgag cggatcatgc ccggtgcatg
      781 aaaaatgcgt tgaacaatcc cggccgatcg agtaggcatg gggcgaaaag actgggacgt
      841 taagaccgtg cgaaggagct aggggagggg cagttcacat gaagcgatcg gtgaagacca
      901 tccagtacgt tctcatcgcc gtcctgcttg cggcaggggc ggcgttcacg ttcgtctacc
      961 tcgagtggga aaagcccgtc atcgaggtgg acaggccgtt cgacaccatc ggctcggcac
     1021 gcagcgtggc ggtggtgatg aaggacgcca agagcggcat ccgttcctac gaggtcgcgc
     1081 tcgtccaggg cgggaagcgc ttcatcgtcg agggcgctga ccttcccgaa aagggcttcc
     1141 acgacaagag gctccagctt gagatctcgc ccggcaggct cggcgtcaag gacggtgagg
     1201 ccgtcttctc cgtgagggtg accgacttct cgccgctgag aaacaccgcc gtgtacgagg
     1261 cgaaggtcaa ggtcgaccag gtgcctccgc gcatagccgt cctgagcagg gcccacaacg
     1321 tgaacgcggg cggcacgtgt ctcgtggtgt accgggtcac caaggaggtc gccgcaagcg
     1381 gcgtgaagtg ccaggactcc tttttccccg gctacgaggt ccggggccaa aagggcagct
     1441 acttcatcgc gtatttcgcg gtcccccgcg acatgacgcc ttccaccccc gttgccgtga
     1501 ccgcggtcga caagggcggg aacagcgctg tcgcggcggt ccccgtgtac ctcaggaaga
     1561 cggcgccctt caggagcgac accgtggcgg tgagcgactc cttcctgcag cagaaggccg
     1621 ccgagttcca gcaggctgac agccgcctcg cgaacaagag cccacaggag atcttctcgt
     1681 tcgtgaacag taccgtgagg caggagaacg acgcaaagat caggtccatc tgcgcgcggt
     1741 ccgcgccgga gaggatatgg gacggcgacg cgtttctgcg gatgaagaac gccgcgccca
     1801 aggcgctctt cggggacgag aggacgtacg tctaccgggg ggtgcccctg ggctc
//
LOCUS       DAOSCR010000556         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42179_length_1845_cov_3.339665, whole genome shotgun sequence.
ACCESSION   DAOSCR010000556 DAOSCR010000000
VERSION     DAOSCR010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42179_length_1845_cov_3.339665"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLT69_11515"
     CDS             complement(<1..290)
                     /locus_tag="PLT69_11515"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOJ15112.1"
                     /translation="MGGVVTLAELKDILEAEVVSGHERLDEIVSGAFCADLMSDVLSF
                     AKPGCVLITGLANLQALRTAYALDLGAVIVCRGKALPEGFVDAARDLGIPVL"
     gene            complement(337..981)
                     /locus_tag="PLT69_11520"
     CDS             complement(337..981)
                     /locus_tag="PLT69_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOJ15113.1"
                     /translation="MVADIIKEIFSHNAAFASSRDERYFGAHMKAQHPKITLVCCCDA
                     RVHATVIAPDPIDRVFTIETIGNQMASAQGSVDYGVLHLHTPLLLILGHTDCGAVKAF
                     MKGYEQENEAIRRELDHLKETIPTPGTGSFEEALVANVKQNVLRQVDKALERYRALVE
                     EGRLTVMGAVYDFTGEFGKGLGKVSILSVNGKTDPAELASMPQLAGLDLGNCLA"
     gene            1439..>1845
                     /locus_tag="PLT69_11525"
     CDS             1439..>1845
                     /locus_tag="PLT69_11525"
                     /inference="COORDINATES: protein motif:HMM:NF037053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ15114.1"
                     /translation="MIISMKKGATKEQIEHVKTLIESFGYRVHESVGAERTLLGAIGD
                     ERGKERLTCLEAQPGVERVIRILSPYKLASGETKESPTVVDIGFGVKVGGPRIAVIAG
                     PCAVESREQIMEVAHAIKESGAHALRGGAFKPRT"
BASE COUNT          348 a          569 c          578 g          350 t
ORIGIN      
        1 ctgagaacgg gtatgccgag atcccgggcg gcatccacga aaccttccgg gagggccttc
       61 cccctgcata cgatcaccgc acccagatcg agcgcatagg cggtcctcag ggcctggagg
      121 ttcgcaaggc cggtgatgag gacgcagccg ggctttgcga acgagaggac gtcgctcatg
      181 aggtccgcgc agaaggcgcc ggagacgatc tcatcgagcc tttcgtgacc cgataccacc
      241 tccgcctcca ggatatcctt cagctcggcg agcgtcacga caccacccat cctcttgccg
      301 gaacaccccc tgacacgggc aggccttgcc cgtgtcctac gcaagacaat tcccgaggtc
      361 caggcctgca agctggggca tggaagcgag ctctgcggga tcggtcttcc cgttgacgct
      421 caggatcgag acttttccga gccccttgcc gaactcaccg gtgaagtcgt acaccgcgcc
      481 cataaccgtg agcctgccct cctcgacgag tgcacggtaa cgctcgaggg ccttgtcgac
      541 ctgcctcaac acgttctgct tcacgttggc cacgagcgcc tcctcgaaac ttcccgtacc
      601 gggggtcggg atggtctcct tgagatggtc gagctccctc cgtatggcct cgttttcctg
      661 ctcgtacccc ttcatgaagg ccttcacggc gccgcagtcg gtgtggccga ggatgagcaa
      721 gaggggcgtg tggagatgaa gcaccccgta atccaccgac ccctgggcgg atgccatctg
      781 attgccgatg gtctcgatgg tgaagaccct gtcgatgggg tccggggcga tgacggtggc
      841 atgcaccctc gcatcgcagc aacagaccag ggtgatcttg gggtgctggg ccttcatgtg
      901 cgccccgaag tatcgctcgt cacggctcga tgcgaacgcg gcgttgtgtg agaatatctc
      961 cttgatgatg tcggcaacca cggtgatacc tcccggatcg tcttttctcc catttatgtg
     1021 cgaaaacacc ctggttgtaa acctgtttca tgcttccgtc acgccgtggg ccggccaagg
     1081 cgcgcctgcc tggaggcctg gctcgaggac tgcaggcatc tcacgactga aggaacacgg
     1141 tgcttcacag ccgtggctcg cgtggagaag gtgatccatg acgggcggga cttcgcccca
     1201 cacgtttccc ccttgcctgc aggggagatc tatgctatgg atcttgttca tgatgaacgc
     1261 ttcggcccac atgttttacg gctattattt ctggtttacc ggggtgtggc cacctgtggg
     1321 ctgaactgtt cgttctgcgg acaagaaggc cgtgggtggt cacctcccac ggccttttcc
     1381 tttgaggcca tcgggaagtg aacacggcac ggcgagaaac aaacgaaagg agactcacgt
     1441 gatcatttcc atgaagaaag gcgccacaaa ggaacagatc gagcatgtca agaccctcat
     1501 cgagtctttc ggataccggg tgcacgagag cgtgggggcg gagaggaccc tgctcggggc
     1561 catcggggac gagcgcggca aggagcgtct cacctgcctc gaggcgcagc ctggggtcga
     1621 gcgcgtcatc aggatcctga gcccgtacaa gctcgcatcc ggggagacca aggagtcccc
     1681 caccgtggtc gacatcgggt tcggcgtgaa ggtgggcggc ccccgcatag cggtcatcgc
     1741 aggcccctgc gccgtggagt cgagggaaca gatcatggag gtggcccatg ccatcaagga
     1801 gagcggcgcc cacgcgctca ggggcggcgc cttcaagccg aggac
//
LOCUS       DAOSCR010000557         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42230_length_1843_cov_3.722036, whole genome shotgun sequence.
ACCESSION   DAOSCR010000557 DAOSCR010000000
VERSION     DAOSCR010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42230_length_1843_cov_3.722036"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLT69_11530"
     CDS             complement(<1..1254)
                     /locus_tag="PLT69_11530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ15115.1"
                     /translation="MNVLVHIDNAVRIFEADLDEPTIMSIEADLTLPNPKWEQVQTYG
                     RGRRPNFQSEFLRFYTRKNGVLIMPRGYIHTLRKRLSGTPHVLVDRTRALDEVDFTFT
                     SSLHPFQAKALKDILSRRFGVLEAPPGAGKTVIALAAIARRRQPALVIVHTKELMYQW
                     QKRACEHLDIDPDEVGLIGDGHRRVGGRLTVALINSLSKVISEVRQRVGHLVVDECHH
                     VPARTFTDAVSLFDTTYMLGLSATPYRRDKLTSLIHFYLGDNTHTIQPQELQALKRIM
                     RARLIVRQTGCAFSFSADEYQRMIAALVTDERRNRLIVEDVHRRASLGMGISLVISDR
                     VSHCETLFRAISSRGIEARLLTGGVPVRERARIVEELNDEKAQVLVATSQLIGEGFDL
                     RQLSSIFLATPIKFTGRVKQYIGRIL"
     gene            complement(1268..>1843)
                     /locus_tag="PLT69_11535"
     CDS             complement(1268..>1843)
                     /locus_tag="PLT69_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ15116.1"
                     /translation="MIERIDHVSLAVRDIDGASRFFTDILGAVPGASAADDRLGYSWR
                     IFSLGDLSRFEIIAPTGRQSFLENFLKGRNGGIHHITLQTPDIAAARRFLEEKGVPYF
                     GYAEYGDLWKELFIHPKDAFGVLVQIAEFNADDWLAPSVRMPGTGKVSVARERDGVTL
                     TLRHPGGGTAKAFLNAGEARSLLEELDKALA"
BASE COUNT          291 a          582 c          605 g          365 t
ORIGIN      
        1 gaggatcctg cctatgtact gcttgaccct gccggtgaac ttgatgggcg tggccaagaa
       61 gatcgacgaa agctgcctga ggtcgaaacc ctcccctatg agctgggagg tggccaccag
      121 gacctgcgcc ttctcgtcgt tgagctcctc cacgatcctg gcgcgttccc tcacgggcac
      181 cccgccggtc agcagcctcg cctcgatgcc ccgggagctg atggccctga agagcgtctc
      241 gcagtgggag accctgtcgg atatgacgag ggatatgccc atgcctagag acgcgcgccg
      301 atgcacgtcc tccacgatga gcctgttcct cctctcgtcc gtcacgagtg cggcgatcat
      361 gcgctggtac tcgtcggcgc tgaaggagaa ggcacacccg gtctgcctca cgatgagccg
      421 tgcgcgcatg attctcttga gcgcctggag ctcttggggc tggatcgtgt gggtgttgtc
      481 gccgaggtag aagtggatga ggctcgtgag cttgtccctg cggtacgggg tcgcggacag
      541 ccccagcatg taggtcgtgt cgaagaggct caccgcgtcc gtgaaggtgc gggccggcac
      601 gtggtggcac tcgtccacca cgaggtgtcc cacccgctgc cgcacctcgc ttatgacctt
      661 ggagagggag ttgatgagcg ccacggtgag cctccctccc acccgcctgt ggccgtcgcc
      721 gatgagcccc acctcgtccg ggtcgatgtc gaggtgctcg caggccctct tctgccactg
      781 gtacatgagt tccttggtgt gcacgatcac gagagcgggc tgcctccttc gcgcgattgc
      841 agccagcgcg atcaccgtct tgccggcccc cggcggggcc tcgagcaccc cgaaccgtcg
      901 cgaaaggatg tccttcaggg ccttggcctg gaaggggtgc aggctgctcg tgaaggtgaa
      961 gtccacctcg tcgagggccc gcgtccggtc gacgagcacg tggggtgtgc ccgagagccg
     1021 tttcctgagg gtgtggatgt accctctcgg catgatgagg acgccgttct tcctcgtgta
     1081 gaacctcaag aactcgctct ggaaattcgg cctcctgccc ctgccgtagg tctgtacctg
     1141 ttcccacttg gggttcggca gggtgaggtc cgcctcgatc gacatgatcg tcggttcgtc
     1201 gaggtccgcc tcgaagatcc gtaccgcgtt gtcgatatgg acgaggacgt tcatctcccc
     1261 cggtgggtca ggccagggcc ttgtccagtt cttccaagag tgaacgggcc tctcctgcat
     1321 tgaggaaggc cttcgcggta ccgccgccgg ggtgcctgag cgtcagggtc acgccgtctc
     1381 tctcgcgcgc gaccgagacc ttgcccgtgc ccggcatcct caccgagggg gcgagccagt
     1441 cgtcggcgtt gaactcggcg atctggacga gcacgccgaa ggcgtccttc gggtggatga
     1501 agagctcttt ccagaggtcc ccgtactcgg cgtacccgaa gtacgggacg cctttttcct
     1561 cgaggaaccg cctcgccgca gcgatgtccg gcgtctgcag ggtgatgtgg tggatccctc
     1621 cgttgcgtcc tttcaggaag ttctcgagga aggactgtcg gcccgttggg gcgatgatct
     1681 cgaaccggct caaatccccg agggaaaaga tccgccagga gtagccgagc cggtcgtccg
     1741 ccgcggacgc gccggggacg gcgcccagga tgtcagtgaa gaagcgggat gcgccgtcga
     1801 tatccctgac cgcgagagat acatggtcga tcctttctat cat
//
LOCUS       DAOSCR010000558         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42304_length_1841_cov_3.731243, whole genome shotgun sequence.
ACCESSION   DAOSCR010000558 DAOSCR010000000
VERSION     DAOSCR010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42304_length_1841_cov_3.731243"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..535)
                     /locus_tag="PLT69_11540"
     CDS             complement(110..535)
                     /locus_tag="PLT69_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15117.1"
                     /translation="MKNRPRRFTGILRACPLLLTLVFAMGMGQAPGVSRTPADSPFDA
                     NITDASRTKVHLASVTFDGDTTLGVTLGKGRVQIPLENISRIEVKDGDLCVTFKDAKT
                     MCGLKAGGSTRVSGKTDYGTYKILLKDVSSIDIVRKDSR"
     gene            complement(532..750)
                     /locus_tag="PLT69_11545"
     CDS             complement(532..750)
                     /locus_tag="PLT69_11545"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HOJ15118.1"
                     /translation="MEFREMSIKVARREIAFVHYVIEAYEGLATVSTLDASRGLLGLA
                     YPASLESTVLDLVEDLRVQGIVKEVHSS"
     gene            1480..>1841
                     /locus_tag="PLT69_11550"
     CDS             1480..>1841
                     /locus_tag="PLT69_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOJ15119.1"
                     /translation="MIEINFTLLIQAANFLLLIFLLNVVLYKPILKILEEREHRIDGQ
                     QAEAKKITDDSQALIDEYNRKLHEAKIEAMNAKNAARSQASEEAGRIIEEARKKAEEM
                     VAQVQREIASEIAQAKKEL"
BASE COUNT          350 a          540 c          613 g          338 t
ORIGIN      
        1 aggccgccca cgagcgccac cagcgaggac gcgctcttga tgaacccctg gtagacgccc
       61 aggcccgtga acaccacgac gaggaggatg gtggccgcgt cgatcgcgtt caccgtgagt
      121 ccttcctcac gatgtcgatg ctcgagacgt ccttgagcag gatcttgtag gtcccgtaat
      181 cggttttccc cgagaccctg gtcgacccgc ccgccttgag gccgcacatg gtcttggcgt
      241 ccttgaacgt cacgcagaga tccccgtcct tcacctcgat cctcgagatg ttctcgaggg
      301 ggatctgcac cctgcccttc ccgagggtga cgcccagggt cgtgtcgccg tcgaaggtga
      361 ccgaggcgag gtggaccttg gtcctcgaag cgtcggtgat gttcgcgtcg aaaggcgagt
      421 cggcgggcgt ccgtgagacg cccggggcct ggcccatgcc catggcgaag acgagggtga
      481 gcagcagcgg gcacgcgcgt aggatacctg tgaaccttct cgggcggttc ttcatgagct
      541 atgcacctcc ttgacgatcc cctggacgcg aaggtcctcc acgagatcga gcacggtcga
      601 ctccaggctt gcggggtagg cgagcccgag caggcctctg gacgcgtcca gggtgctcac
      661 cgtggcgaga ccttcgtatg cctcgatcac gtagtggacg aacgcgatct ccctccgggc
      721 gaccttgatc gacatttccc ggaattccac catggtttcc tgagttatca cacaccgtct
      781 gcgagcacaa cattccagaa ggagcctgtt tttgtcgtgc acgcgatcgg tgactgtgcg
      841 cggcgcccat ccttggggat ttatccccgt gcccaagatc atgccccggg gtgggggtgc
      901 ggcgccaggc cgccacccgc ctggtccacg ggctggggcg tccccgaggc ggcgctcccg
      961 caggcccgag agtccctgcc tgcggagggg ggcagaaggc acaggggaaa ggtttcgttc
     1021 gagaaacgaa agtccctgcc tgggtatagg aacaggcctt cgtacctgcc ggcggacccc
     1081 ggcatgcgtt cctcccatgg tgcggtgaac atggttctcg gtccgggagc gggtttcggc
     1141 cgtcaacaaa attcttgaca tgaacggatg cgcggtgata gtggaattgg gtctgaatga
     1201 gtgaacagcc attcagtcag ggccgtcgtg ggtgatgcgc accgaccaag gggtggagac
     1261 aggttcgtgt cggtggaacc gaatggtctc ctgtcagggt tcgggtgccg ggcccggcgg
     1321 tgactcgtcg gaggacggcc gcctccacca ggggcagctt gggacggaat gacaaggggc
     1381 gagcttgtcg ggcgtttcgc gatcctgagc ctcgagcatg gttccgtctc tggtggaaag
     1441 gtgggttccc tctcggacca acgtcgaagg aggctggttt tgatagagat aaacttcacc
     1501 cttctgatcc aggctgccaa tttccttctc ctcatcttcc tgttgaatgt ggtcctctac
     1561 aagcctatcc tgaagatcct cgaggagaga gagcacagga tcgacggcca gcaggccgag
     1621 gccaagaaga tcacggatga cagccaggcg ttgatcgatg aatacaaccg gaagctgcac
     1681 gaggccaaga tcgaggccat gaacgcgaag aacgcggcca ggagtcaggc atccgaggag
     1741 gcgggccgca tcatagaaga ggccaggaag aaggccgagg agatggtcgc ccaggtccag
     1801 cgggagatcg cctcggagat agcgcaggcc aagaaggagc t
//
LOCUS       DAOSCR010000559         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42305_length_1841_cov_3.268757, whole genome shotgun sequence.
ACCESSION   DAOSCR010000559 DAOSCR010000000
VERSION     DAOSCR010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42305_length_1841_cov_3.268757"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLT69_11555"
     CDS             complement(<1..528)
                     /locus_tag="PLT69_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15120.1"
                     /translation="MRSKSAIKEYLFIIREMVSDVEELIEDRADEDACVEWKSRALGI
                     LKRILGSEHAYVKEFDSIKLTTDFFDDFDDLVREQTLTIAMDDAHSFLVALMDELESE
                     HSSTPGMMDMESMFAEMNRYVVSHVGDPMTRSTLHNRITRLREGMMTGEISGDEARHH
                     IEHIGYLDAGLYERLV"
     gene            604..1290
                     /locus_tag="PLT69_11560"
     CDS             604..1290
                     /locus_tag="PLT69_11560"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOJ15121.1"
                     /translation="MGFSPVTDLLGRPDELRAFIKEVLARRERRTIPAGSLREAAVLV
                     PLCWRDPGPAVLLTKRSLDVEHHKGEISFPGGRFEPGDRDIVHTALREAHEEIGLAGD
                     DVDVLGLLDDHVSIVGYHITPVVGSIPYPYPFRINSESEALIELELARILDGDAWMAE
                     RSFFKGSSVNMFYLETDQGVVWGATARMLKHFADLLAGTTVPWGEVSASARTWVMNLI
                     QAQGAYRDLR"
BASE COUNT          332 a          604 c          572 g          333 t
ORIGIN      
        1 cacgaggcgt tcatagagac ccgcgtccag gtaaccgatg tgttcgatat gatgccgggc
       61 ctcgtctccg gatatctcgc ccgtcatcat cccttccctt aggcgggtga ttcggttgtg
      121 gagggtgctc ctggtcatgg ggtctcccac gtgggagacc acgtagcggt tcatctccgc
      181 gaacatgctt tccatgtcca tcatcccagg ggtgctgctg tgctcgctct cgagctcgtc
      241 catgagggcg accaaaaacg agtgggcgtc gtccatggcg atcgtgaggg tctgttccct
      301 cacgaggtcg tcgaaatcgt cgaagaagtc cgtggtgagc tttatggagt cgaactcctt
      361 gacgtaggcg tgctccgaac cgaggatcct cttgaggata cccagagccc ggctcttcca
      421 ctcgacgcac gcgtcttcat cggcccggtc ctcgatgagc tcctccacgt cgctcaccat
      481 ctcccgtatg atgaagagat actcctttat ggcgctcttg cttctcatgc cggcatcata
      541 tacagcatac ctccgaccgg tcaagcctgt tcatggcgcg ccttcgtgtg ctagaatgca
      601 tgcatgggct tctcccccgt gaccgatctc ctcggcaggc ccgacgagtt gagggccttc
      661 atcaaggagg tgctcgcccg ccgagagcgg cggaccatac ctgccggaag cctgcgagag
      721 gccgcggtgc tcgtccccct gtgctggagg gacccggggc ccgccgtcct gctgaccaag
      781 agatccctcg acgtggagca ccacaagggc gagatctcgt tcccgggagg caggttcgag
      841 ccgggcgaca gggacatcgt gcacaccgcc ctacgcgagg ctcacgagga gatcggcctc
      901 gccggcgacg atgtggacgt gctcgggttg ctcgacgacc acgtctccat cgtcggttac
      961 cacataaccc cggtggtcgg ctccatccca tacccgtacc cgtttcgcat caacagcgag
     1021 tcggaagccc tgatcgagct cgagctggcg aggatcctcg acggtgatgc atggatggcg
     1081 gaacgctcgt tcttcaaggg gagctcggtg aacatgttct atctcgagac cgaccagggg
     1141 gtggtctggg gggccaccgc gcgcatgctc aagcacttcg ccgacctcct ggccgggacc
     1201 accgtaccct ggggcgaggt gtcggccagc gccaggacgt gggtgatgaa cctcatccaa
     1261 gcccaggggg cgtacaggga cctgcgctga gagtgccgtc tcgaggccag aaacgcgcga
     1321 acgcctgaca cgcggagacg ttgcccgtca accatgcctc gaaccgacac gaccatgatc
     1381 ctgccggggt gctccagagg cacccagaga cccggggcgc ccccggcgcg cgcccctggg
     1441 tctcgctgcc gggaggcctg cctgcctcag gtacgagaaa tgcccaggcg gtcctcatga
     1501 agcgacggat cgtgccgata cgtctttgtg ggaagggaga cacgctggga tattcgacag
     1561 accggacccc tgaggcggac taaccgaaca cgagacagtg tcatcgtaga cgaaaagaga
     1621 gtccggccgg gctgagtcac cggtatcccc ggcatctccc cttttccttt ttccggggga
     1681 aaagcccgcc ggggagaccg cacccggagg tcctccccaa aacgccccca cccgcgcgct
     1741 ccggtcaacc gacaccacga agggctaggc ggcttccggt ggccgttctc gcatcgacgg
     1801 ccaagggccc tgcggcatcg gtccaccggg aaagcccatg g
//
LOCUS       DAOSCR010000560         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42368_length_1839_cov_2.918161, whole genome shotgun sequence.
ACCESSION   DAOSCR010000560 DAOSCR010000000
VERSION     DAOSCR010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42368_length_1839_cov_2.918161"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLT69_11565"
     CDS             complement(<1..821)
                     /locus_tag="PLT69_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ15122.1"
                     /translation="MRSRTTIVASGSMVLGSSGLMKAYLGSCVGVAAIDRRRHMGGLL
                     HVLLPKPLCDVPETQLSSYATTGVPLFLKALSERGASPDELETYVAGGALGESPTRED
                     LFMNIGGRTLEAAMETLADNGFHARILEAGGLAGLCMNLDMDRTTCTIEPIIDEIRSR
                     PRKETPLPTLAEIGRSIENLRPIPQIAIRVSEMLSEDNTDISTVASEIKKDQVLSADV
                     LRLCNSPYLGIHRRVDSIDDAVTILGTTTLLQLVMTAHIERLVGCSENGYSLMRGG"
     gene            complement(1491..>1839)
                     /locus_tag="PLT69_11570"
     CDS             complement(1491..>1839)
                     /locus_tag="PLT69_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOJ15123.1"
                     /translation="KNQTLTSGKLKDAGTILRDMNDEDRELLEGIVREFHEDFLDKVR
                     HRRPVSESDLEVIADGRVMTASVGMQHHLVDEVGYYEDALKAVERLANVSNPTVVVYR
                     RRTENRGGFYSWP"
BASE COUNT          286 a          595 c          620 g          338 t
ORIGIN      
        1 cccccgcgca tgagcgaata cccgttctca gaacacccca cgaggcgttc gatgtgggcc
       61 gtcatgacga gctgcaggag ggtcgtggtg cccaggatcg tgaccgcgtc gtctatggaa
      121 tcgacgcgcc tgtggatgcc caggtatggc gagttgcaca gcctgaggac gtcggcgctg
      181 agcacctggt ccttcttgat ctccgaggcg acggtcgaga tgtcggtgtt gtcttccgag
      241 agcatctccg agaccctgat cgcgatctgc gggatgggcc tgaggttctc gatggacctc
      301 cctatctccg ctaacgtcgg caagggggtc tccttcctgg gtctcgaacg gatctcgtcg
      361 atgatcggct cgatcgtgca cgttgtcctg tccatgtcca ggttcatgca caacccggcc
      421 agcccgcccg cctcgaggat cctcgcatgg aagccgttgt ccgcgagggt ctccatggcc
      481 gcttccagcg tcctgccccc gatgttcatg aacaggtctt cccgggtggg actctctccg
      541 agtgcgccgc cggcgacata ggtctcgagc tcgtccggcg aggccccgcg ctctgacaag
      601 gccttgagga acaaggggac cccggtcgtg gcgtaggatg agagctgggt ctcgggcacg
      661 tcgcagaggg gcttgggaag gagcacgtgg agcaggcccc ccatgtgccg cctcctgtcg
      721 atcgccgcta cacccacgca ggagccgagg tacgccttca tcaggcccga cgagcccagt
      781 accatgctcc cgcttgccac gatcgtggta cgggatctca tgaaaccttc tccttcatgt
      841 ccgggacaca cccccccgcc ggacttcgtc ctgaatcact agatcggacg cccgacccac
      901 gaagatgagg gggccgggca aacgggtttc agacctggac gggcgctcct catggaccga
      961 cagggtcgtc gaaggtgagg ggtgggcgct cgagggtatc gaaccgtcgc atcccccagg
     1021 gcatccatgg gtctttgcgt ggcgaccccg aagggagcgt cgagacggtg gtccgtccat
     1081 gccgagggcc cgcctcgtgc gaaaggcgcg tgatcccttt ggcagggcgt gcccacctgc
     1141 ctttctgctc gtgcccgtgg gagcggtggc cctagacgct gcagccgaca gggggcctcg
     1201 gcccaaagag ggctggcaag gaggcgccca cggcaaaggt agggcggtcg ggcgtcttcc
     1261 gggacggcgg cgcagccccc ggccccttgg aggaggccgc tgcccggtgg catcggcttc
     1321 aagacgcgag ggggagggcc ctcctgcgcg gaagtcggct caaggcgggt aggcgctagg
     1381 ggagtgttgt cggcatgcgg gaaggcgccc tgcgcgaaaa acggcccgag accggccatg
     1441 aggggtcctt cgatgcgtca cggcgccgga caccctgcgt ggtggcgggc tcaaggccag
     1501 gagtaaaacc cgcccctgtt ctcggtccgc ctccggtaga ccaccaccgt ggggttgctc
     1561 acgttcgcga gacgctcgac ggccttgagg gcgtcctcgt agtacccgac ctcgtcgacg
     1621 aggtggtgct gcatgccgac ggatgccgtc atgaccctcc cgtccgctat gacctccagg
     1681 tcgctttcgc tcaccggcct tctgtgcctg accttgtcga ggaagtcctc gtggaactcg
     1741 cgcacgatgc cctcgaggag ttccctgtcc tcgtcgttca tgtccctgag gattgtgccc
     1801 gcatccttga gcttgccgga ggtgagggtc tggttcttg
//
LOCUS       DAOSCR010000561         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42436_length_1837_cov_2.433782, whole genome shotgun sequence.
ACCESSION   DAOSCR010000561 DAOSCR010000000
VERSION     DAOSCR010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42436_length_1837_cov_2.433782"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLT69_11575"
     CDS             <1..470
                     /locus_tag="PLT69_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ15124.1"
                     /translation="GLPSKLPFQIYTKNSRYVPDENKAKEASACSRYMAVVNGSGLCL
                     FGVFLGVTRTPTFDWLNAVTGWNKAPEEYLEIGWRIQTLRQAFNIRHGIDPNGNKLHD
                     RALGRPPLVEGANKNRTVLIEEMMRGYWSQLGWDESTGRPLPETLERLGIEAP"
     gene            489..911
                     /locus_tag="PLT69_11580"
     CDS             489..911
                     /locus_tag="PLT69_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ15125.1"
                     /translation="MPYTITDACVGCQACVRVCPVGAITGEKKAKHVIDHELCIACGA
                     CGRICPHASVLDPSGALCTMEKRSSWKKPAVDEKACVSCMVCIETCPVNCLALSAAKD
                     SGDPHGYPYLKDAGSCIACGFCAAECPVAAIRMVQPPA"
     gene            complement(1135..1836)
                     /locus_tag="PLT69_11585"
     CDS             complement(1135..1836)
                     /locus_tag="PLT69_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ15126.1"
                     /translation="MFFVQLVSCLGRPPFFLKQYFKQMEFIGIRSTPVVMLTGIFTGM
                     VSALQTYHGFSLFGAESLVGSTTALGLFRELGPVLGALMVTARAGSAMTAEIGIMQVT
                     QQVDALVVMGVNPFKYLITPRITASIVMMPMLTLIFDAIGMIGASFVGTVILDIDRGV
                     FMSRIVDYVEYEDLFSGLVKACFFGAILSVVGCYKGYSTTGGAEGVGRAATQSVVISS
                     VSILIADYLLTAVMF"
BASE COUNT          370 a          549 c          622 g          296 t
ORIGIN      
        1 aagggcttcc gtccaagctg cccttccaga tatacacgaa gaacagcagg tacgtgcccg
       61 acgagaacaa ggcgaaagag gcctcggcct gcagcaggta catggcggtc gtgaacgggt
      121 caggcctttg cctgttcggc gtgttcctgg gcgtcaccag gaccccgacc ttcgactggc
      181 tcaacgcggt caccggctgg aacaaggcgc ccgaggaata cctggagata ggctggagga
      241 tccagacgct gaggcaggcg ttcaacatca ggcacggcat cgatccgaac ggcaacaagc
      301 tccatgacag ggccctgggc aggccgccgc tcgtcgaggg ggcgaacaag aacaggaccg
      361 tcctgatcga ggagatgatg agggggtact ggtcgcaact cggctgggac gagtccacgg
      421 gcaggccgtt gccggagacg ctcgagagac tgggcatcga ggccccgtga caaggaggag
      481 gaagagacat gccctacaca atcacggacg catgcgttgg gtgccaggcc tgcgtgaggg
      541 tttgcccggt cggtgcgatc accggtgaga agaaggcgaa gcacgtcatc gaccatgagc
      601 tgtgcatagc gtgcggcgca tgcggcagaa tctgcccgca cgcaagcgtg ctggacccct
      661 cgggcgccct gtgcaccatg gagaagagat cctcgtggaa gaaacccgct gtcgacgaga
      721 aggcctgcgt gtcgtgcatg gtatgcatcg agacctgccc ggtgaattgt ctcgccctct
      781 cggccgcgaa agacagcggc gacccgcacg gctaccccta cctcaaggat gccggttcat
      841 gcattgcatg cggcttctgc gccgccgagt gcccggtggc cgcgataagg atggttcagc
      901 ccccggccta gaccttcggc tgctcaggct tattttcgga aggcacgggg aggcccgagg
      961 ccgttgatcg ggccgcgggg ctcgatgcgg cctctcgcgg gatggtgcgg cggccgtgac
     1021 cttgaagcag gtgcctttcg aggcccgcat cctcatgccc gaggaaagcc ctctcggaag
     1081 gggcacggcc ggggccgtgc ctgctctccg ggttgtgatc gtgggaggct cgtctcagaa
     1141 catgaccgcc gtgaggaggt agtccgcgat caggatgctc accgaggaga tgaccaccga
     1201 ctgggtggcg gccctgccca cgccttccgc cccgcctgtg gtgctgtacc ccttgtaaca
     1261 gcccacgacc gacaggatgg ccccgaagaa gcaggccttg acgagcccgg agaagaggtc
     1321 ctcgtactcg acgtaatcca cgatgcgcga catgaacacg cctctgtcga tgtcgaggat
     1381 gaccgtgccc acgaatgaag ccccgatcat gcctatggcg tcgaagatga gggtcagcat
     1441 gggcatcatg acgatcgagg cggttatgcg gggcgttatg aggtacttga aagggttgac
     1501 gcccatgacc acgagggcgt cgacctgctg cgtgacctgc atgatgccga tctccgcggt
     1561 catggcgctt cccgcccgtg cagtcaccat gagggccccg agcaccggcc cgagttccct
     1621 gaaaagaccg agggcggtgg tggaacccac gagcgattcg gcgccgaaca ggctgaaccc
     1681 gtggtaggtc tggagtgcgc tcaccatgcc ggtgaagatg ccggtgagca tcaccacggg
     1741 ggtcgaccgg atgcctatga actccatctg cttgaaatac tgcttcagga agaacggcgg
     1801 cctgccgagg caggagacga gctgcacgaa gaacagg
//
LOCUS       DAOSCR010000562         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42580_length_1833_cov_2.653543, whole genome shotgun sequence.
ACCESSION   DAOSCR010000562 DAOSCR010000000
VERSION     DAOSCR010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42580_length_1833_cov_2.653543"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLT69_11590"
     CDS             complement(<1..448)
                     /locus_tag="PLT69_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HOJ15127.1"
                     /translation="MRKTHALVLMSALVIAPALLHAAEGVTIPSVTLGVKEAGRPEDV
                     VGALQILFILTILALAPSIVMMVTAFTRIVVVFGFLRQALGTQQMPPMQVLSALAIFL
                     TFFIMSPTLDSVNELALKPYRDGTITFGQALKNAEGPLREFMFKQTR"
     gene            complement(445..828)
                     /gene="fliO"
                     /locus_tag="PLT69_11595"
     CDS             complement(445..828)
                     /gene="fliO"
                     /locus_tag="PLT69_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOJ15128.1"
                     /translation="MKTHPAALAALTLSWASPLQAATGVGAGDALRATLALVFVVGLM
                     FALAWAARRFGPVIRAERGLGVRVLGHVALGQRASLALVRLGGAILLLGVTQNSVNLL
                     KEMEASDFESALARSQGREVEGGNP"
     gene            complement(825..1310)
                     /gene="fliN"
                     /locus_tag="PLT69_11600"
     CDS             complement(825..1310)
                     /gene="fliN"
                     /locus_tag="PLT69_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOJ15129.1"
                     /translation="MAEEEKTGETLSQDDIDKLLGGEGSSGAVDQEKLAEEWAQSLES
                     GKPMAEEGSPDRGARKASFAELDEPRPSSGQAKDLDFILDIPLEVTVEMGRTRMLIND
                     LLQVGQGSVIELNRLAGEPLDILINGKLIARGEVVVVSEKFGIRITDIVSPLERVKRL
                     S"
     gene            complement(1321..>1833)
                     /locus_tag="PLT69_11605"
     CDS             complement(1321..>1833)
                     /locus_tag="PLT69_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOJ15130.1"
                     /translation="QSGIRDLMNAWQPVYELEFHYVRSEMNPQFAGIIPNEDVIVVVQ
                     FEVELDEMTGNIMVCMPYMLIEPIRDRLYATFHSEEKASVDSAWVNRLKNELMGVYVD
                     IEVELGRSRIAPRELASLRPGNVIVLDKDVTEPLVVSVQRLPKFYARPGKIKDTLAID
                     ILGAIDAKLY"
BASE COUNT          303 a          627 c          546 g          357 t
ORIGIN      
        1 tgcgcgtctg cttgaacatg aactccctga gcgggccctc ggcgttcttc aaggcctggc
       61 cgaaggtgat ggtgccgtcc ctgtagggct tgagcgcgag ctcgttgacg ctgtcgaggg
      121 tcggggacat gatgaagaac gtcaaaaaaa tggcgagggc cgaaaggacc tgcatgggtg
      181 gcatctgctg ggttcccagg gcctgtctca gaaacccgaa caccaccacg atcctggtga
      241 acgcggtgac catcatcacg atggagggtg cgagcgcgag gatggtcagg atgaacagta
      301 tctggagggc cccgaccacg tcttccggcc ttcccgcctc cttcaccccg agggtgacgc
      361 tcgggatcgt caccccctct gccgcatgga gcagggccgg cgcgatcacg agggcggaca
      421 tgagcaccag ggcgtgtgtc tttctcacgg gttccccccc tccacctcgc ggccctgcga
      481 ccgggcgagg gcgctttcga agtccgaggc ctccatctcc ttgaggaggt tgaccgagtt
      541 ctgggtcacg cccaagagga gtatcgcccc ccccagcctg accagcgcca ggctcgccct
      601 ctggcccagc gccacgtgac cgagcaccct gacgcccagg cccctctcgg cgcggatgac
      661 cggcccgaac ctcctcgcgg cccacgcgag cgcgaacatg agcccgacca cgaacacgag
      721 cgcaagcgtc gccctgaggg catcgccggc gcccacgccg gttgcggcct gcagggggga
      781 ggcccacgag agcgtcaggg ctgcaagcgc tgcaggatgg gttttcacga gagcctcttc
      841 acacgttcga gcgggctcac gatgtccgtg atccttatcc cgaacttctc gctcaccacg
      901 acgacctcgc ccctggcgat gagcttgccg ttgatcagga tgtccagcgg ctcccccgcc
      961 agcctgttga gctctatgac agagccctgc cccacctgga ggagatcgtt gatgagcatc
     1021 ctcgtcctcc ccatctcgac cgtcacctcg agggggatgt cgaggatgaa gtcgagatcc
     1081 ttggcctgcc cggatgaggg cctgggctcg tccagctccg cgaaggacgc ctttctcgcc
     1141 cccctgtccg gggacccttc ctcggccatg ggcttcccgc tctcgaggct ctgggcccac
     1201 tcctcggcga gcttttcctg gtccactgcg ccgctcgacc cctctccccc caggagtttg
     1261 tcgatatcgt cctggctgag cgtctcgccg gtcttttctt cctctgccat ggtttcctcc
     1321 ttagtagagc ttcgcgtcta tggcgccgag aatatcgatc gcgagggtgt cctttatctt
     1381 tcccggcctc gcgtagaact tcggcagtct ctgcacgctg acgacgagcg gttcggtcac
     1441 gtccttgtcc agcacgatca cgttgcccgg cctgagggaa gcgagctcgc ggggcgcgat
     1501 cctcgaacgg ccgagttcca cttctatgtc cacgtagaca cccatgagct cgttcttgag
     1561 cctgttcacc cacgcggagt caacagacgc cttctcctcg gagtggaagg tcgcatacag
     1621 cctgtcccgg atgggctcta tgagcatgta cggcatgcac accatgatgt tcccggtcat
     1681 ctcgtccagc tcaacctcga actggaccac cacgatcacg tcctcgttgg gtatgatgcc
     1741 cgcgaactgc gggttcatct cggacctcac gtaatggaac tcgagctcgt agacgggctg
     1801 ccacgcgttc atgaggtccc tgatgcccga ctg
//
LOCUS       DAOSCR010000563         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42610_length_1832_cov_2.795723, whole genome shotgun sequence.
ACCESSION   DAOSCR010000563 DAOSCR010000000
VERSION     DAOSCR010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42610_length_1832_cov_2.795723"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLT69_11610"
     CDS             complement(<1..280)
                     /locus_tag="PLT69_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HOJ15131.1"
                     /translation="MRVLVIGSGGREHALCWKIAQSPLVDEVFCAPGNAGIAEDAVCV
                     DIGSDDTAGLLRFAKDNAFDLTVVGPEAPLVGGIADAFLAEGLPVFGPG"
     gene            complement(291..>1832)
                     /gene="purH"
                     /locus_tag="PLT69_11615"
     CDS             complement(291..>1832)
                     /gene="purH"
                     /locus_tag="PLT69_11615"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOJ15132.1"
                     /translation="VTDKGGISDFVKALASFGVEIVSTGGTAKILRDAGVEIVDISDF
                     TGFPEMMDGRLKTLHPLVHGGMLALRDNPEHVRAMEEHGIRPIDMLVVNLYRFEETVA
                     RGASLEEAVENIDIGGPAMVRAASKNYRFVTVVTDPGDYASIVEEMRRTGGAVSEETN
                     FELARKAFSLTARYDAAISNYLQSVGVDHPKFPLTYTTQFRRRQIMRYGENPHQDAAF
                     YVEWGLDQPSVASAEQLWGKELSYNNIMDADAALDLVMEFDTPACVILKHSNPCGAAL
                     SDASLEEAYRKALAVDPTSAFGGIVGFNRTVDAPTARALSEVFTEVVLAPDYTRDALD
                     ILREKKNIRLLRVPGIEAGASAKKPFLVSRRVTGGLLLQDRDLGRVDLGKAEVVTKRG
                     PTPEELKALEFAWRIVKYVKSNAVVYAKAGQLVGVGAGQMSRVDSSRLAVSKAREAGL
                     STQGSVVASDAFFPFRDGVDEAAKAGATAIVQPGGSVRDAEVVEAADEHGMAMIFTGM
                     RHFRH"
BASE COUNT          283 a          648 c          585 g          316 t
ORIGIN      
        1 cgccgggacc gaacacgggc aggccctccg cgaggaaggc gtccgcgatc ccgccgacca
       61 ggggcgcctc gggccccacc accgtcaggt cgaacgcgtt gtccttggcg aacctcagga
      121 gcccggccgt gtcgtcggaa ccgatatcca cgcacaccgc gtcctcggcg atgcccgcat
      181 tgccgggcgc gcagaagacc tcgtcgacga gggggctctg ggcgatcttc cagcacaggg
      241 cgtgttcccg tccccccgac ccgatgacca ggacccgcat gggatacctc ctagtgcctg
      301 aagtggcgca tgccggtgaa gatcatggcc atgccgtgct cgtcagcggc ctcgacgacc
      361 tcggcgtcgc gcaccgagcc gccgggctgg acgatggccg tggcgcctgc cttggcagcc
      421 tcgtccaccc cgtccctgaa ggggaagaag gcgtctgagg cgaccaccga accctgcgtg
      481 gacagccccg cttcccgcgc cttggacacg gcgagcctcg atgagtcgac cctgctcatc
      541 tggcccgccc ccacgcccac gagctggccc gccttcgcat agaccacggc gttcgacttg
      601 acgtacttga cgatcctcca ggcgaactcc agtgccttga gctcctcagg cgtcgggccc
      661 ctcttcgtca ccacctcggc cttgcccagg tccacccggc ccaggtcacg gtcctgcagg
      721 agcagacctc ccgtcacccg cctggagacg aggaaaggtt tcttcgcgct cgcgcccgcc
      781 tcgatccccg ggaccctgag gagcctgatg ttcttcttct ccctgaggat gtcgagggcg
      841 tcccgggtgt agtccggcgc aaggaccacc tcggtgaaca cctccgagag ggcccgcgcc
      901 gtgggggcgt cgaccgtcct gttgaacccc acgatcccgc cgaaggcgct cgtcgggtcg
      961 accgcgaggg ccttccggta cgcctcctcg agggatgcgt ccgagagcgc cgccccgcac
     1021 gggttcgagt gcttgaggat gacgcacgca ggggtgtcga actccatgac cagatcgagg
     1081 gccgcgtccg cgtccatgat gttgttgtac gagagctcct tgccccagag ctgttccgcg
     1141 gaggcaacgg acggctggtc gagaccccat tcgacgtaga aggccgcatc ctggtgcggg
     1201 ttctccccgt agcgcatgat ctgcctgcgc ctgaactgcg tcgtgtaggt gagggggaac
     1261 ttggggtgat cgacgcccac cgactggagg tagttggaga tcgcggcgtc gtagcgggcc
     1321 gtcagcgaga aggccttccg ggcgagctcg aagttggtct cctcgctcac ggcaccaccg
     1381 gtccttctca tttcctcgac gatggacgcg tagtctccgg ggtcggtcac gaccgtgacg
     1441 aacctgtagt tcttggatgc ggccctcacc atggccggcc ccccgatgtc gatgttctcg
     1501 accgcctcct cgagggaagc gcccctcgcc accgtctcct cgaagcggta caggttgacg
     1561 acgagcatgt cgatgggcct gatgccgtgc tcctccatgg cgcgcacgtg ttccgggttg
     1621 tccctgaggg cgagcatgcc gccgtgcacc agggggtgca gggtcttcag cctgccgtcc
     1681 atcatctcgg ggaaacccgt gaagtcggag atatcgacga tctcgacccc ggcatccctc
     1741 aggatcttcg ccgtcccgcc cgtggagacg atctccacgc cgaaggaagc gagggccttc
     1801 acgaagtcgc ttatcccgcc cttgtcggtg ac
//
LOCUS       DAOSCR010000564         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42615_length_1832_cov_2.576252, whole genome shotgun sequence.
ACCESSION   DAOSCR010000564 DAOSCR010000000
VERSION     DAOSCR010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42615_length_1832_cov_2.576252"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /gene="uvrA"
                     /locus_tag="PLT69_11620"
     CDS             <1..1577
                     /gene="uvrA"
                     /locus_tag="PLT69_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ15133.1"
                     /translation="HLGGMKTQEELFEGLSLEGKEQDIASRLVKEIKDRLAFMSSVGI
                     GYLTLGRASGTLSSGEAQRIRLATQIGSALMGVLYILDEPTIGLHQRDNDRLIETLKH
                     LRDLGNTLVVVEHDEDTIMNADYVVDMGPGAGELGGEVVFEGPPEDLLGHPLSLTGKY
                     LSGRLSIHIPEKRRRPKGPRLRIRGASTNNLKGIDVDIPLGLFTCITGVSGSGKSSLV
                     IDTLYPYLRAVLARERIPQVKVASIGGAEYIDRVINVDQSPIGRTPRSNPATYTGLFT
                     PVRELFAMLPDAKARGYRPGRFSFNVKGGRCETCEGGGLIRIEMHFLPDVYVVCDQCR
                     GTRFNEETLSVKYKGRSIADVLDMTVRQALEFFDAVPAIRRRLKTLVDVGMDYIRLGQ
                     SATTLSGGEAQRTKLARELSKRSSGRTLYILDEPTTGLHFDDVNKLLVVLHRLVDEGN
                     TVVVIEHNMDIIASADHVIDLGPEGGDSGGYVVAQGSPEEIAGESASHTGRYLAAHLE
                     KKRLKHVVQQAQGVRA"
BASE COUNT          356 a          564 c          612 g          300 t
ORIGIN      
        1 accatctcgg ggggatgaaa acccaggaag agctcttcga gggcctgagc ctcgagggca
       61 aagagcaaga catcgcgtcc aggctcgtca aggagatcaa ggacaggctt gccttcatga
      121 gctccgtggg gataggctat ctcacgctcg gcagggcgtc ggggaccctg tcgtcaggcg
      181 aggctcagcg catacggctc gccacccaga tagggtcggc gctcatgggt gtcctctaca
      241 tcctcgacga gcccaccata gggctccacc agcgcgacaa cgaccggctc atagaaacgc
      301 tcaaacacct gagggatctc gggaacacct tggtggtggt ggaacacgac gaggacacga
      361 tcatgaacgc cgactacgtg gtggacatgg gccccggtgc aggcgaactg ggcggcgagg
      421 tggtcttcga gggtccgccc gaggacctgc tcggtcatcc gctctccctc accgggaagt
      481 atctctccgg gagactgtcc atccacatcc ccgagaagag gcgcaggccg aaggggcctc
      541 gcctgaggat caggggggct tccacgaaca acctcaaggg gatagacgtc gatatccctc
      601 tcgggctctt cacgtgcatc accggggtct cggggtccgg gaagtcgagc ctcgtcatcg
      661 acacgctcta cccgtacctg agggcggtcc ttgcccggga gaggatcccc caggtcaagg
      721 tggcctccat cgggggcgca gagtacatcg accgcgtcat caacgtcgac cagtcgccga
      781 tcggacgaac gccccgctcg aacccggcca cgtacacggg gctcttcacc cccgtgaggg
      841 aactcttcgc catgctgccg gatgccaagg cgagggggta ccggccgggc aggttcagct
      901 tcaacgtcaa gggcggtagg tgcgagacgt gcgagggcgg cgggctcatc aggatagaga
      961 tgcactttct gcccgacgtg tacgtggtct gcgaccagtg caggggcacc cggttcaacg
     1021 aggagaccct gtccgtcaag tacaaggggc gttccatcgc ggatgtcctc gacatgaccg
     1081 tgcgccaggc cctggagttc ttcgacgccg tcccggccat caggcgcagg ctgaagaccc
     1141 tcgtggacgt ggggatggac tatatacggt tgggacagag cgccacgacg ctctccggcg
     1201 gcgaggccca gcggacaaag ctcgccaggg agctgtccaa gcgatcgagc ggccgtacgc
     1261 tctacatcct cgacgagccc accaccggtc tccacttcga cgacgtcaac aagctgctcg
     1321 tggttctcca ccgcctcgtg gacgagggga acacggtcgt ggtgatcgag cacaacatgg
     1381 acatcatagc ctctgccgac cacgtgatcg acctcggtcc cgaggggggc gactcggggg
     1441 ggtacgtggt ggcccagggg agccccgagg agatcgcggg agagtccgca tcgcacaccg
     1501 gacgctacct cgccgcccac ctcgagaaga agcgcctcaa gcatgtcgtt cagcaggcac
     1561 agggggtgcg cgcatgaacg aggcgggggg gtgcgggtga ggccggtctt tccccggcgc
     1621 cgttgaccat gccttcaatc tgttctagag taaaagccaa gggaaacaac accctgtttg
     1681 aaggtcgtgg agcagtccct gccatggctt gggaggagag tcagtgagga gagtctcggc
     1741 cgtcgtcctg tttgtgtgtg cgttgtccgt cgtgctggcc cccggcgtgg ctcgatgcga
     1801 tgagaggaag acccgcatag aggtcctcca gg
//
LOCUS       DAOSCR010000565         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42644_length_1831_cov_2.432995, whole genome shotgun sequence.
ACCESSION   DAOSCR010000565 DAOSCR010000000
VERSION     DAOSCR010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42644_length_1831_cov_2.432995"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="PLT69_11625"
     CDS             <1..1424
                     /locus_tag="PLT69_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15134.1"
                     /translation="PAFRELLGYSEEELATMGLKDIVRPELAVMIETRYEKRIRGEKV
                     PDQYEIALRSKDGAWRAFEITPSIIEYEGRPATQNVIRDITQRREAQKALRASEAKYR
                     ATVEHTGTAMMILDEDYTITFANNQLERLSGYTKEEIVGKMRWTELVHEEDVERMKQY
                     SRARRTGDKDVPSQYEFRFVDKNGAVRDAFLTIGMIPGTRQSIVSIMDITEIKRMQRE
                     LEHARRMAVLGEMSAHVAHEVRNPLQKIKTGVELLSASLDMDERQRRQLQGVKDGVEN
                     LEKFVTQILEWTRSGEIKPKTHRISNIMDGLLFNMADALEQRRVVVKTDYDPQADTLE
                     IDGIQMRQVFVNIIENALDAMPEGGTLHVITRRLEHEGKEASGGTGGVAAPIVEIRVL
                     DTGCGIPAQERQKVFHPFYTRKAKGTGLGLALVKKVIDMHKGEVHVEEAPGGGTALVI
                     RLPVRQEGPGGITGVGDAERGAR"
     gene            1439..>1831
                     /locus_tag="PLT69_11630"
     CDS             1439..>1831
                     /locus_tag="PLT69_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15135.1"
                     /translation="MSERKRVLLVDDEEVFLEQLKEALENSRLDLEIETASDGLEALQ
                     KIEARRHDIVITDLKMPRMDGHALLREIHSRFPTTYVVVVTAFGSVSAAVEAMKYGAY
                     DFLEKPFNMETLEISITKILRQHEILREN"
BASE COUNT          411 a          544 c          625 g          251 t
ORIGIN      
        1 acccggcctt cagggagctc ctcggctact ccgaggagga gctggcgacc atgggcctca
       61 aggatatcgt caggcccgag ctcgccgtca tgatcgagac caggtacgaa aagcgcatca
      121 ggggggagaa ggtcccggac cagtacgaga tcgccctgag gtcgaaggac ggcgcttgga
      181 gggccttcga gatcacgccg tcgatcatcg agtatgaggg caggcctgcg acacagaacg
      241 tgatccggga catcacccag aggagggagg cccagaaggc gctcagggcc agcgaggcga
      301 agtaccgcgc cacggtcgag cacacgggca cggccatgat gatcctcgac gaggactaca
      361 cgatcacctt cgcgaacaac cagttggagc gcctcagcgg gtacacgaag gaggagatcg
      421 tcgggaagat gcgctggacg gaactcgtcc acgaggagga cgtggagcgc atgaagcagt
      481 attcgcgcgc caggcgcacg ggggacaagg acgtgccctc gcagtacgag ttccgtttcg
      541 tcgacaagaa cggcgcggtc agggatgcgt tcctcaccat cggcatgatc ccgggaacca
      601 ggcagagcat cgtgtccatc atggacatca ccgagatcaa gcgcatgcag cgcgagctcg
      661 agcatgcccg gaggatggcg gtgctggggg agatgtcggc tcacgtggcc cacgaggtgc
      721 gtaaccccct ccagaagata aagaccgggg tggagctgct ctcggcctcg ctcgacatgg
      781 acgagaggca gcgcaggcag ctgcaggggg tgaaggacgg ggtggaaaac ctcgagaagt
      841 tcgtgacgca gatcctcgaa tggaccaggt ccggcgagat caagccgaag acgcacagga
      901 tcagcaacat catggacggc ctcctgttca acatggccga cgcgctcgag cagcgccgcg
      961 tggtcgtgaa gaccgattac gacccccagg ccgacaccct ggagatagac ggcatccaga
     1021 tgcgccaggt gttcgtcaac atcatcgaga acgccctgga tgccatgcca gagggaggca
     1081 ccctccacgt gatcacgcgc cgactcgagc atgaagggaa agaggcctcc ggcgggacgg
     1141 ggggcgtggc ggccccgatc gtggagatcc gggtcctgga caccgggtgc ggcatccccg
     1201 cccaggagcg ccagaaggtg ttccatccgt tctacacgag aaaggccaaa ggcaccggcc
     1261 tggggctcgc cctcgtcaag aaggtgatcg acatgcacaa gggtgaggtc cacgtcgagg
     1321 aggccccagg cggcgggacg gcgctcgtca tccggctgcc agtccgccag gagggccccg
     1381 gggggatcac gggcgtcggt gacgccgagc gaggcgctcg atgaggagaa gcgaggaaat
     1441 gtccgagagg aagagggtcc tcctcgtgga cgacgaggag gtgtttctcg aacagctcaa
     1501 ggaggccctc gagaactcca ggctcgacct ggagatcgag accgccagcg acggcttgga
     1561 agccctgcaa aagatcgagg cgcgcaggca cgatatcgtg atcaccgacc tcaagatgcc
     1621 gcgcatggac ggccatgccc tcctcaggga gatccacagc cgtttcccga cgacctacgt
     1681 ggtcgtcgtc accgcgttcg ggagcgtctc cgccgcggtc gaggcgatga agtacggggc
     1741 ctatgacttc ctcgagaaac ccttcaacat ggagaccctc gagatatcga tcaccaagat
     1801 cctcaggcag cacgagatcc tcagggagaa c
//
LOCUS       DAOSCR010000566         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_42917_length_1823_cov_2.125000, whole genome shotgun sequence.
ACCESSION   DAOSCR010000566 DAOSCR010000000
VERSION     DAOSCR010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42917_length_1823_cov_2.125000"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PLT69_11635"
     CDS             <1..1737
                     /locus_tag="PLT69_11635"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOJ15136.1"
                     /translation="SSLALVDLNSTNVLPSHLGVTTDAERDELLDFVKGQGKFHPKTG
                     DDVWKRDWILGDIIHSQPTILYDRDRNRNVIFVGANDGFLHCFIDSDRGTPSLLDDEV
                     REAWAYVPGDLLKNLKYLPSDNMTSAGTLGDTYHDYFVDGSPVLYKSGGSRYLAFGLR
                     RGGKDLQTGGELDNQYFILRLNASDDNVTPTLVARIAKDILGSNPDDEKLGQSWCTPV
                     FATIKTGPAESNRQDVLLLTGGYDTNQDGDDPGGSDSKGRAVFAVGAASGALVSSLNF
                     NHKNFPQMRYSMVDLMAYDNDDDGCTDVIYAPSVGGDLFLFDDAHQNLGSVNDGTWTR
                     RLLFRSNSMGGTSRLRKFMYAPAIVQETWGDWVYIASGDREHPSDPTGSGYGGYNRIY
                     AIRNTFPSTWNNDSPFTDGDLSDVSADELQGTVSSPSTLSEEQKMTLRYQLANTGNGW
                     FFDLERPGEKAVSSPVVYKGVVYFTTYTPGRPSTEEDPCGTTGGGIARIYAVDYRTGE
                     AVFATFDGNAAKLTKEDRYKDVGSGIPSEPVVIVTKAGTYVLVSTEEGTQPIKTGDPV
                     GLNRYYWLKRVD"
BASE COUNT          378 a          559 c          582 g          304 t
ORIGIN      
        1 agcagcctgg cgctcgtcga tctcaacagc acgaacgtcc tgccctcgca cctgggggtc
       61 acgaccgatg ctgagcgcga cgagcttctg gacttcgtga aggggcaggg aaagttccac
      121 ccgaagaccg gggatgacgt gtggaagagg gactggatcc tgggggacat cattcactcc
      181 cagccgacca tactctacga cagggacagg aacaggaacg tgatcttcgt gggtgccaac
      241 gacggcttcc tccactgctt catcgacagc gacaggggaa caccgtcgct cctggacgac
      301 gaggtgaggg aggcatgggc gtatgtcccc ggcgatctcc tgaagaacct caagtatctg
      361 ccctctgaca acatgacgtc ggccggtacg ctcggggaca cgtaccacga ttacttcgtg
      421 gatggatccc cggtcctgta caagagcgga gggtcccgct acctggcctt cggcctgagg
      481 aggggcggca aggatctcca gaccgggggc gagctcgaca accagtactt catcctgagg
      541 ttgaacgctt ctgatgacaa cgtcaccccg acgctcgtgg cgaggatcgc gaaggacata
      601 ctcggaagca acccggacga cgagaagctc gggcagtcgt ggtgcacgcc cgtcttcgcg
      661 accataaaaa ccgggccggc cgagtccaac aggcaggatg tcctgctcct taccgggggc
      721 tacgacacga accaggacgg ggacgacccg ggcggctcgg actcgaaggg gagggcggtc
      781 ttcgcggtgg gtgcggccag cggcgccctc gtgagcagcc tgaacttcaa ccacaagaat
      841 ttcccgcaga tgcgttactc catggtggac ctgatggcct acgacaacga cgacgacggg
      901 tgtaccgacg tcatatacgc gccgtcggtg gggggcgacc tgttcctctt cgacgacgca
      961 caccagaacc tgggtagcgt caacgacggc acatggacgc gcaggctgct cttcaggtcg
     1021 aactccatgg gcggcacttc caggctcagg aaattcatgt acgccccggc gatcgtccag
     1081 gagacgtggg gcgactgggt ctacatcgcc agcggcgaca gggaacaccc ctcggatccg
     1141 accggcagcg gctacggcgg gtacaacagg atctatgcca tcagaaatac cttcccttcc
     1201 acctggaaca acgactctcc cttcacagac ggcgatctct cggacgtgag cgccgacgag
     1261 ctgcagggta ccgtgtcgag tccctcgacc ctgtccgagg aacagaagat gacattgagg
     1321 taccaactcg ccaacacggg caacgggtgg ttcttcgacc tcgagcgccc cggcgagaag
     1381 gcggtctctt ctcctgtggt gtacaagggc gtggtgtact tcacgaccta cacgcccggc
     1441 cgtccctcca ccgaggagga cccgtgcgga acgaccggcg ggggcatcgc ccgcatctac
     1501 gccgtcgact acaggacggg tgaggccgtg ttcgccacgt tcgacggcaa cgccgcgaaa
     1561 ctcacgaagg aggacaggta caaggatgtc ggctcgggca tcccttccga gccggtggtc
     1621 atcgtcacga aggcggggac gtacgtgttg gtctccacgg aagaggggac ccaacccata
     1681 aaaaccggag atcccgtggg cctcaacagg tactactggc tgaagagggt ggactgaaag
     1741 gggtgcggtt atgaagacgt actgtggtct tgtcgctgct tcctgcgcga tcgcgtgggc
     1801 gctcgtgctt tccctgcatg ccc
//
LOCUS       DAOSCR010000567         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_43418_length_1807_cov_4.327626, whole genome shotgun sequence.
ACCESSION   DAOSCR010000567 DAOSCR010000000
VERSION     DAOSCR010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43418_length_1807_cov_4.327626"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLT69_11640"
     CDS             <1..750
                     /locus_tag="PLT69_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLAC1 anion channel family protein"
                     /protein_id="HOJ15137.1"
                     /translation="AVRREVRHPVTLSFFPAVSIGILLMSVAFLNTNTELSFLLWVVG
                     TILHAGLTLYIMREWITQEHFEIGHMNPAWFIPVVGNLIVPLAGVTHAPADISWFFFS
                     IGIVMWVVLFTVVTYRMIFHRVIPERLLPTLFILVAPPAVGFIAYVKLAGGLDAFGRI
                     LFFFAVFMFAFLMTLGARLARIRFFLSWWTYTFPVAALVAACTLMGRLAPSVFFTGMA
                     VVLWVLLSLFILFLGARTAISITRREICVEE"
     gene            833..>1807
                     /locus_tag="PLT69_11645"
     CDS             833..>1807
                     /locus_tag="PLT69_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ15138.1"
                     /translation="MKRDHEHGISRRAFLSGVATAAVSGAVGLPAKARAAGEGAFGTL
                     VDLSLCDGCEGRDMPACVEACRTKNAGVVPEPVDPIPKPFPRGKIEDWSKKQGVTDRL
                     TPYNYLFIQKAEVELGGAKKTVFIPRRCMHCDNPACATICPFSANHKYESGAVVIDQD
                     ICFGGAKCKEVCPWAIPERQSGVGIYLHILPTLAGNGVMFKCDLCQDLLLKGEDPVCI
                     TACPRKAMLIGPKEQILAEAERRAEAMNGYIYGKEENGGTSTLYVSPVPFELLDAAIE
                     KGPGRPHLGKAPRKMAKTDPVAKAVMASPVIGLVAGLAAVAGARMKSGK"
BASE COUNT          325 a          554 c          576 g          352 t
ORIGIN      
        1 gcggtacgca gggaggtgag gcatcccgtc acgctcagct tcttccccgc ggtctccatc
       61 ggcatcctgc tcatgagcgt ggcgttcttg aacactaaca ccgagctgtc attcctcctc
      121 tgggtggtgg ggaccatcct gcatgcgggc ctgaccctgt acatcatgcg ggagtggatc
      181 acccaggaac acttcgagat cggtcacatg aacccggcgt ggttcattcc cgtggtggga
      241 aacctgatcg ttcctttggc aggggtgacc catgcgcctg ccgacatctc gtggtttttc
      301 ttttccatcg ggatcgtcat gtgggtcgtg ctcttcaccg tggtcaccta ccggatgatc
      361 tttcaccgag ttatcccgga gaggctgctg cccacgctgt tcatcctcgt ggcgccgcct
      421 gccgtgggat tcatagccta cgtgaagctc gcaggaggac tggatgcctt cggcaggatc
      481 ctgttcttct tcgccgtgtt catgttcgcg tttttgatga cccttggtgc aaggctcgcc
      541 aggatcaggt tcttcctgtc ctggtggacc tacacgttcc cggtggctgc cttggtcgca
      601 gcgtgcacgc tcatggggcg tctggccccg agcgttttct ttacgggcat ggcggtcgtc
      661 ctgtgggtcc tgctgtcctt gttcattctg ttcctcgggg caaggacagc gatctccata
      721 acgcgcaggg agatctgcgt ggaggaatga ggcgaagaaa ccgggggctt ctccatgaaa
      781 cgctctgtgc ttactataca cggtggacaa tccaagaagg agaggacagg gtatgaagag
      841 agatcatgaa cacggcatct ccaggagggc gttcctttcg ggcgtcgcca cggcggccgt
      901 ctcgggcgcc gtgggtctgc ccgcaaaggc tcgtgcagcc ggggaaggcg ccttcggcac
      961 cctcgtcgac ctcagcctgt gcgacgggtg cgagggcagg gacatgcccg cctgcgtcga
     1021 ggcgtgcaga acaaagaatg cgggcgtggt gcccgaaccc gtggatccca tccccaagcc
     1081 cttccccagg ggcaagatcg aggactggtc aaagaagcag ggggtgactg accgcctcac
     1141 gccgtacaac tacctcttta tccagaaggc ggaggtggag ctgggcggcg cgaagaagac
     1201 cgtctttatc cccaggcggt gcatgcactg cgacaacccc gcctgcgcca ccatctgccc
     1261 cttctctgcc aaccacaagt acgaatccgg cgccgtggtg atcgaccagg acatctgctt
     1321 cggcggcgcc aagtgcaagg aggtgtgccc ctgggccatc cccgagcggc agtcgggcgt
     1381 gggcatctac ctgcacatcc tgccgacgct cgcgggcaac ggcgtcatgt tcaagtgcga
     1441 cctctgccag gacctgctcc tgaaagggga agacccagtg tgcatcacgg cgtgcccgag
     1501 aaaggccatg ctcatcgggc cgaaggaaca gatcctcgcc gaggccgagc gccgggccga
     1561 ggccatgaac gggtacatct atggaaagga agagaacggg ggaacctcaa ccctgtacgt
     1621 ctctcccgtg cccttcgagc tcttggacgc ggcaatcgag aaaggcccgg gcaggcccca
     1681 cctgggcaag gcgccgagaa agatggccaa gacagacccc gttgccaagg ccgtcatggc
     1741 ctcgccggtg atcgggctcg tggccggtct cgcagccgtg gcgggcgcgc ggatgaagtc
     1801 cggaaag
//
LOCUS       DAOSCR010000568         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_43426_length_1807_cov_3.227740, whole genome shotgun sequence.
ACCESSION   DAOSCR010000568 DAOSCR010000000
VERSION     DAOSCR010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43426_length_1807_cov_3.227740"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLT69_11650"
     CDS             <1..192
                     /locus_tag="PLT69_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15139.1"
                     /translation="SSNARPRGPEGKGHHGARKVVRRGDPLPEDRLEARLPAWRGHWH
                     ASASLDRTFSVFLVDLFSR"
     gene            879..>1807
                     /gene="msrA"
                     /locus_tag="PLT69_11655"
     CDS             879..>1807
                     /gene="msrA"
                     /locus_tag="PLT69_11655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOJ15140.1"
                     /translation="MLIITPTTGGTTDAKYETATFAGGCFWCMEAAFEEVPGVIEAVS
                     GYTGGTTKNPTYEEVSSGTTGHVEAVRVTFDPARVTYAQLLDVFWRHIDPTDDGGQFV
                     DRGSQYRSGIFYHSEEQRKLAEASRAALEKSGRFGKPVVTRIAKAGAFYPAEDYHQGY
                     SRKNPVRYRIYSRNSGRPRYLEEVWGGRDTGTKKQPPPAPGTYRKPDRAVLKKILTPL
                     QYEVTQEGGTEPPFRNEYWDNHREGIYVDVVSGEPLFSSLDKFDSGTGWPSFVRPLVP
                     ENIVERKDTSLFMTRTEVRSAHADSHLGHVFDDG"
BASE COUNT          323 a          584 c          615 g          285 t
ORIGIN      
        1 tcatcgaacg caaggccaag agggcctgag ggaaagggcc accacggcgc acggaaggtc
       61 gtgcgcaggg gtgaccctct tcctgaggac aggctggagg cacggctgcc cgcatggcgc
      121 ggacactggc acgcctctgc aagcctcgat cgaacgttca gcgtcttcct cgtcgacctc
      181 ttctcaagat gacaacggcc cgcacctgga tgctgcccgg gttcccactt gtgcccgtcc
      241 ggtccgcacg gggcctgggc cttgtgcgca gacgccctgt gtctccccgt gagagaggcc
      301 cctcccggcg agccaggcgt cccgtgcccg tcctgtcccc atggggcctg agcctttgtt
      361 gtgcatcccc gcgagagcga caagtcccag cgggcccgga ggcctggtgg agctgggcgt
      421 tcaagcgccc ggttgtgtgt tcccgcagcg aggctgttcc ctatccccgg tgttgttccc
      481 tggcaaggtg acgggagttg cccgcctgcc tggtcgcagc gaggctgttc cccatcttcg
      541 tcggtgctcg ggggcgggcc tgggcacaga tctcgtctcc ttgtgttcgt gagcccttgc
      601 aatcgggagg ggcggaccct acgaagcgtc ttacacggag agccgaaggc tccctggcat
      661 gttcctccag gtgcatggcg gtgtgcgcgg ctgccgcagg cctgcaggcc ttaacgccag
      721 ggcgcgcgcg aagcccatac agggctcctg cggccgtggc ccctgccggc cggcatcgcc
      781 gtggcccctg ccaaaagggg acgttcatgc aggtgcgagg gctgcagggg ggaagggaaa
      841 aggggatgtt cacgaagatc atgaaaggga tgctcctcat gctcatcatc acaccgacca
      901 caggcggcac gacagatgcg aagtacgaga cagcgacctt tgccgggggc tgcttctggt
      961 gcatggaggc ggccttcgag gaggtgccgg gcgtcatcga ggcggtctcg ggatacacgg
     1021 gcgggaccac gaagaacccc acctacgagg aggtgtcctc ggggacgacc gggcacgtcg
     1081 aggcggtccg ggtgaccttc gacccggcca gggtgaccta cgctcagctg ctcgacgtgt
     1141 tctggcggca catcgacccc acggacgacg ggggccagtt cgtggaccgc gggtcccagt
     1201 accgaagcgg gatcttctac cactccgaag agcagaggaa actcgccgag gcatccaggg
     1261 ctgccctcga gaagtccggg agattcggca agcccgtcgt caccaggata gcgaaggccg
     1321 gggccttcta cccggccgag gactatcacc agggctactc caggaagaac cccgtgcggt
     1381 acaggatcta cagccgcaac tcgggacgcc cccggtacct tgaggaggtg tggggcggca
     1441 gggacacggg cacgaagaaa cagccgcccc ctgcacccgg cacgtaccgg aaacccgaca
     1501 gggccgtctt gaagaagatc ctgacgccgc tgcagtacga ggtcacccag gaggggggca
     1561 ccgagccgcc cttcaggaac gaatactggg acaaccaccg cgagggcatc tacgttgacg
     1621 tggtctccgg cgagcccctg ttcagctccc tggacaagtt cgactccggt accggctggc
     1681 cgagcttcgt aaggccgctc gtgcccgaga acatcgtgga gagaaaggac acgagccttt
     1741 tcatgaccag gactgaggtg aggagcgctc acgccgactc ccacctgggg cacgtcttcg
     1801 acgacgg
//
LOCUS       DAOSCR010000569         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_43455_length_1806_cov_3.335237, whole genome shotgun sequence.
ACCESSION   DAOSCR010000569 DAOSCR010000000
VERSION     DAOSCR010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43455_length_1806_cov_3.335237"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /gene="carB"
                     /locus_tag="PLT69_11660"
     CDS             complement(<1..1712)
                     /gene="carB"
                     /locus_tag="PLT69_11660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ15141.1"
                     /translation="MPRRTDIKKVMIIGSGPIVIGQACEFDYSGTQACKALRSLGYEI
                     VLVNSNPATIMTDPQMADATYIEPLNVTSLESIIARERPDAILPNLGGQSGLNLTSEL
                     HRKGILERYGVKVIGVNIDAIERGEDRSAFKETMERLGIEMPKSALAYSVEEAERIAL
                     ELGYPVVIRPAYTLGGTGGGLVYNVEELRLVAGRGLSASLIGQVLVEESVLGWEELEL
                     EVVRDAKNNMITVCFIENVDAMGVHTGDSFCTAPMLTIPRELQERLQKHAYDIVEAIE
                     VIGGTNVQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIALVSSRLASGLTLDEM
                     PYWRLGTLDKYEPWGDYVVVKFARWAFEKFRGVIDRLGTQMRSVGEVMSIGKNYKEAL
                     QKAIRSLETGRYGLGFAKNFHELDLGTLMDMLGEPTSERQFIMYEALRKGASVEELSR
                     KTHLKAWFIEQMKELVELEERILACRESGLPDDLLVRAKKDGFADRYLAMLLGVSEEQ
                     IRERRKRLGVVHGYDKVPVSGVERGEYYYSTYNAPDVVQTSDRPKIMVLGGGPNRIGQ
                     GIEF"
BASE COUNT          292 a          582 c          558 g          374 t
ORIGIN      
        1 tcgaactcga tgccctgccc gatgcggttg gggccgccgc ccaggaccat gatcttgggc
       61 ctgtcagacg tctggacgac gtcgggcgcg ttgtaggtgg agtagtagta ttcccctcgt
      121 tcgacgccgc tgaccgggac tttgtcgtat ccgtgcacga cgcccagccg cttgcggcgc
      181 tcgcggatct gctcctcgga gacgccgagc agcatggcga ggtacctgtc cgcgaacccg
      241 tctttcttgg cgcggacgag caggtcgtcc ggcaggccgc tttcgcggca ggcaaggatc
      301 ctttcctcga gctccacgag ttccttcatc tgctcgatga accatgcctt gaggtgggtc
      361 ttcctcgaga gctcctccac cgaggcgccc ttcctcaagg cctcgtacat gatgaactgc
      421 ctctcgctcg ttggctcccc cagcatgtcc atgagggtgc cgaggtcgag ctcgtggaag
      481 ttcttcgcga acccgaggcc gtacctgcct gtctcgagcg acctgatcgc cttctggagc
      541 gcttccttgt agttcttccc gatgctcatg acctcgccca cggagcgcat ctgggtgccg
      601 agcctgtcga tgacccccct gaacttctcg aaggcccacc tggcgaactt gaccaccacg
      661 tagtcacccc agggctcgta cttgtccaac gtgcctaagc gccagtacgg catctcgtcc
      721 agcgtcaggc ccgatgcgag cctggaggag acgagggcga tggggaagcc cgtcgccttg
      781 gaggcgagcg ccgatgacct cgaggtgcgc gggttgatct cgatcacgac gactcggccc
      841 gtcttggggt cgtgggcgaa ctggacgttg gtccccccga tgacctctat cgcctccacg
      901 atgtcgtagg cgtgtttctg gaggcgctcc tggagctcgc gcggaatggt gagcatgggc
      961 gccgtgcaga acgagtcgcc ggtgtgcacg cccatggcgt cgacgttctc gatgaagcac
     1021 accgtgatca tgttgttctt ggcgtcgcgc accacctcga gctcgagttc ctcccagccc
     1081 agcaccgatt cctcgacgag gacctggccg atgaggctcg ccgagaggcc cctgcccgcc
     1141 accaacctga gctcctccac gttgtacacg aggccgcctc ccgttccccc cagcgtgtag
     1201 gccggcctga tgaccaccgg gtaaccgagc tccaaggcga tgcgctcggc ctcctcaacg
     1261 ctgtaggcga gcgcgctctt gggcatctcg atgcccaggc gttccatggt ctccttgaag
     1321 gcgctcctgt cttcgcccct ctcgatggcg tcgatgttca cgccgatgac cttcaccccg
     1381 taacgctcga gtatgccctt gcggtggagc tcggaggtga ggttgagccc cgactgtccc
     1441 ccgaggttgg gaaggatggc atccgggcgc tcacgcgcga tgatggactc gagcgaggtc
     1501 acgttgaggg gctcgatgta ggtggcgtcg gccatctggg ggtcggtcat gatggtggcc
     1561 gggttcgagt tgacgagcac gatctcgtag cccagcgacc tcagcgcctt gcacgcctgg
     1621 gtgcccgagt agtcgaactc gcaggcctgg ccgatcacga tgggaccgct tcctatgatc
     1681 atgaccttct tgatgtccgt acgtctcggc atggtgagaa ccccccgaaa gttgagctat
     1741 ggcactataa ataaggttca tgacaacttt ggcacgtgaa aagtgctata agttggctta
     1801 tggaca
//
LOCUS       DAOSCR010000570         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_43488_length_1805_cov_3.156571, whole genome shotgun sequence.
ACCESSION   DAOSCR010000570 DAOSCR010000000
VERSION     DAOSCR010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43488_length_1805_cov_3.156571"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLT69_11665"
     CDS             <1..814
                     /locus_tag="PLT69_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOJ15142.1"
                     /translation="RRACVPGRYVSLDPDTVCSKDSFDIALLAAGSLIRLVEMAVAGE
                     IDCAFASVRPPGHHATPNEAMGFCLFNNVAVAAEKAIASLGVTRVLIVDFDVHHGNGT
                     QDCFYSRKDVLYFSSHQYPFYPGTGGMHETGAEGARGYTVNCPLGARKGDGEFMALYE
                     RVLLPVMRAFEPGLVLVSAGFDAHAMDPIGGMQLSSRGFAALAGLILENARQAGAPVV
                     FSLEGGYNLDALRDSVREVVEVMKGGQAPKVRPSGFRELDDICEVQARYWPV"
     gene            862..1593
                     /locus_tag="PLT69_11670"
     CDS             862..1593
                     /locus_tag="PLT69_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOJ15143.1"
                     /translation="MLDERTIESLEVREFKPGEIIVKEGDPSDRFFAILTGEVQISQL
                     DKPIRILTERDVFGLENYYRGVPYTTTAKATRTSRIAAYESELIDEIAYEKPALAAMI
                     MRSAFLQLEQTTSVAEANITYKDTINLDIREYKDGEVIIEEGTTGTEIFRLYQTEGGL
                     EVLKKGVRIGLISKPGEYFGEMSFILNEPRSATIRSLGTSLVEVIPVAEGGIEGIISE
                     NPEIANRIITTLAARLREANLLLVK"
BASE COUNT          345 a          588 c          553 g          319 t
ORIGIN      
        1 gaggcgcgca tgcgtcccgg gcaggtacgt gagcctcgac ccggacacgg tctgctccaa
       61 ggactcgttc gacatcgcgc ttttggccgc gggctccttg atcaggctcg tggagatggc
      121 cgtggcggga gagatcgatt gcgccttcgc ctccgtcagg ccgcccggtc accacgccac
      181 cccgaacgag gcgatgggct tctgcctgtt caacaacgtg gctgtcgcgg ccgagaaggc
      241 gatcgcgtct ctgggcgtca cccgtgtgct catcgtggat ttcgacgtcc accacggcaa
      301 cggcacccag gattgtttct actccaggaa ggacgtgctc tatttctctt cgcaccagta
      361 tcccttctac ccgggcacgg gcggcatgca tgaaaccggg gccgaagggg cccgggggta
      421 cacggtcaac tgtcccctcg gcgcccgcaa gggcgacggg gagttcatgg cgctctacga
      481 gagggtgctc cttccggtca tgagggcctt cgagcccggc ctcgtcctcg tctccgccgg
      541 cttcgacgcc cacgccatgg atcccatcgg gggcatgcag ctctcatcga ggggcttcgc
      601 cgcgctcgcc ggtctcatcc tcgagaacgc aaggcaggcg ggtgcgcccg tggtgttctc
      661 gctggagggg gggtacaacc tcgacgcact gcgtgattcc gtccgggaag tggttgaggt
      721 tatgaaggga gggcaagccc caaaagtgag gccttctggc ttcagggagc tcgacgacat
      781 ctgcgaggta caggcgcggt actggcccgt ctgagacctc gcgggctcaa gtttggtgca
      841 cgccatgccg aaaggcccgg tatgctcgat gaacgaacca tagaatcgct ggaggttcgc
      901 gaattcaagc ccggtgagat catcgtcaag gagggtgacc cctccgaccg cttcttcgcg
      961 atactcaccg gcgaggtgca gatatcgcag ctcgacaagc ccatacgcat cctcaccgag
     1021 cgcgatgtct tcggcctcga gaactactac aggggtgtgc cgtacacgac cacggcgaag
     1081 gccacccgca cctcgcgcat cgcagcctac gagtcggaac tcatcgacga gatcgcctac
     1141 gaaaagcccg ccctcgccgc catgatcatg cggtccgcct tccttcaact cgaacagacc
     1201 acctcggtcg cagaggcgaa catcacgtac aaggatacca tcaacctgga catccgcgag
     1261 tacaaggacg gcgaggtcat catcgaggaa ggcacgacgg gtaccgagat cttcaggctc
     1321 taccagaccg agggtggtct cgaggtgctc aagaaggggg tccgcatcgg gctcatcagc
     1381 aagcccggcg agtatttcgg cgagatgagc ttcatcctca acgagccccg aagcgccacg
     1441 atccgttccc tgggcacgag cctcgtcgag gtcatccccg tggcggaagg cggtatcgag
     1501 ggcatcatca gcgagaatcc ggagatcgcc aaccgcatca tcaccaccct tgcagcgagg
     1561 ctcagggagg cgaacctcct cctcgtgaag tgacacgtta acaccccggt ctccaagccg
     1621 aggcggttct tctttgggat cgctccagga atcacccctg caggccgaaa gccttctcgc
     1681 catgcccctg ccacctgccc gcagatcgct gcagcgccgg gaatagcccc cgctgacggt
     1741 aacccctcac gtctcttgaa gggcacaggt ctgtgagatg aggcttgtct atactcggga
     1801 agccg
//
LOCUS       DAOSCR010000571         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_43737_length_1798_cov_3.153758, whole genome shotgun sequence.
ACCESSION   DAOSCR010000571 DAOSCR010000000
VERSION     DAOSCR010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43737_length_1798_cov_3.153758"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..733)
                     /locus_tag="PLT69_11675"
     CDS             complement(149..733)
                     /locus_tag="PLT69_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ15144.1"
                     /translation="MQRLFLDNNGVRIEALFEDTGSDWCAVLAHPHSLMGGNMYNNVV
                     ASAWDACLSIGLSALRFNFRGVGSSGGSFDDGRGEMDDLACAAAYPGRPVVVVGYSFG
                     AWVASRHAKRIDTPPPCILVSPPTSLFSFPDMRGDPAWVVSGGSDPFSSPHSLTGIVD
                     ADRITVVRSADHFWFGQEEVLSKVLAGLLGSIVG"
     gene            complement(976..>1798)
                     /locus_tag="PLT69_11680"
     CDS             complement(976..>1798)
                     /locus_tag="PLT69_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOJ15145.1"
                     /translation="AGLLRKAGGARAILKRPEGDRPLIAQIFTSSASEAAQAARVLED
                     EGFDGIDINMGCPVKKVVGKGAGVALMTDPAKALSLVEAVVRSVDLPVSVKLRAGWDA
                     SSMNACECASLFEAVGIDTVVLHPRTRTDMYRGTPRWGELERLARTVKIPVVASGDIR
                     GVEDVERARSLGARAFMVGRGAIGNPWIFRQLLGGGPPSLEEREDVMIRHLDALCSLH
                     GSRLGVLHMRKFLSPYVKGVQGAARFRAQACTIDNPKALEAAVRDFFASQTGKAP"
BASE COUNT          287 a          613 c          609 g          289 t
ORIGIN      
        1 ggtcagcgcc gggaaggggt ggcatgagcg ccgagacctt gtccttcagg gcgggcttcc
       61 ttctccggca gtcctgcaga cggtggtcgt gcgggagctg gctggccctc tctgcggcgc
      121 gggtttgccg cgaccctcaa agggatgctc aacccacgat cgagcccaag agcccggcca
      181 ggaccttcga gagcacctct tcctggccga accagaagtg gtcggcggat ctcaccacgg
      241 tgatgcggtc cgcatcgacg atccccgtga gtgaatgggg gctcgagaag gggtccgagc
      301 cgccggagac gacccacgcg gggtcccccc tcatgtcggg gaacgaaaag agcgaggtcg
      361 ggggcgagac gaggatgcag ggaggcggtg tgtcgatgcg ctttgcgtgc ctggaggcca
      421 cccatgcgcc gaaggagtac cccaccacga cgaccggcct tcccgggtag gccgcggcgc
      481 aggcgaggtc gtccatctcc ccccgcccgt catcgaagga accgccgctc gaacccacgc
      541 cccggaagtt gaaccggagg gccgacagcc cgatcgagag gcaggcatcc catgcggatg
      601 cgaccacgtt gttgtacatg ttgccgccca tgagtgagtg cgggtgggcc agcaccgcgc
      661 accagtccga ccccgtatcc tcgaagaggg cctcgatgcg gacgccgttg ttgtccaaga
      721 agagcctctg catgacgctt ccttgtacct caaccggggc acccggatca acggcctgag
      781 gtgaggccgg gccgtgggat cccctggagg aacacccagc ggagaggtct cgatgcggga
      841 tgtctttccg gggcgcatga tgaggactcc cgcgtcaacc gggagggttc tcgagaagtc
      901 ttgatgaagg agtacgttcc cggggcgcat gatcgggccg ggctcgcggc ctgcccctgc
      961 ggggagctca tcgcatcagg gcgcctttcc cgtctgggat gcgaagaagt ccctcaccgc
     1021 ggcctcgagc gccttggggt tgtcgatggt gcacgcctga gccctgaacc tggccgcacc
     1081 ctggacgcct ttcacgtagg gggagaggaa cttcctcatg tggagcaccc ccaggcgtga
     1141 cccgtggagg gagcacaagg cgtccaggtg cctgatcatc acgtcctcgc gttcctcgag
     1201 ggagggggga ccgcctccga gcagctgcct gaagatccat gggttgccta tggcccccct
     1261 gcccaccatg aaggcccgtg cgcccaggga cctggcgcgc tcgacgtcct cgacgccgcg
     1321 gatgtcgccg ctcgcgacca cggggatctt caccgtcctt gcgagcctct cgagttcccc
     1381 ccacctcggc gtgccgcggt acatgtccgt cctggtcctc gggtgcagga cgacggtgtc
     1441 gatcccgacc gcctcgaaca gggacgcgca ctcgcaggcg ttcatgctcg atgcgtccca
     1501 gccggccctg agcttcacgc tcacgggcag gtcgacggac ctgacgacgg cctcgacgag
     1561 agagagcgcc tttgcggggt cggtcatgag cgcgacgccc gcccccttgc ccacgacctt
     1621 cttcaccggg cagcccatgt tgatgtctat cccgtcgaac ccctcgtcct cgagcacccg
     1681 ggccgcctgt gcggcctcgg aggcggacga ggtgaagatc tgggcgatga ggggcctgtc
     1741 tccctcgggc cgcttcagga tcgccctggc gccccccgcc ttgcgcaaca gcccggcc
//
LOCUS       DAOSCR010000572         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_43913_length_1793_cov_3.759494, whole genome shotgun sequence.
ACCESSION   DAOSCR010000572 DAOSCR010000000
VERSION     DAOSCR010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43913_length_1793_cov_3.759494"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLT69_11685"
     CDS             complement(<1..221)
                     /locus_tag="PLT69_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ15146.1"
                     /translation="MRVYAITGICDVAKTDNPLEPMDLPEPKPAGGEVLLKVRACGVC
                     HTELDEIEGRTPPPVFPTVPGHQVVAEVAA"
     gene            412..>835
                     /locus_tag="PLT69_11690"
     CDS             412..>835
                     /locus_tag="PLT69_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15147.1"
                     /translation="MHRTSASEKGTVSPATLRVLSVRLLVILALLPMAAISCSSDGGG
                     GGGDGSSAVPDELSFSMDLSSDFVDPLTITSYTDSNKSITIASVSGPVSGTYNHATGE
                     YTITEGALVNVRYSLWDEPYRTFTIYLASDVTGSTGQTP"
     assembly_gap    836..845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <846..1509
                     /locus_tag="PLT69_11695"
     CDS             <846..1509
                     /locus_tag="PLT69_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15148.1"
                     /translation="GALEVRSGTDTVTVTISPSGVRLSLDGADPTSTIAWQDLYPMYY
                     DEDEPGWRKEASLACVVLKFLVEEIAFSADTLVTIALNEETLETAGYLEFEGDPLPPS
                     IAFGESTSVLRLTSPSIRDGATFQWLYDWCWDDDPSSSEDLLYDGTVQMSGYTETVDV
                     SGSTRRFTELGFGSGTAPGGVSYDRFMIYVTEETSGSYTIPEARTLVYDGGYSILFTS
                     GD"
BASE COUNT          309 a          637 c          526 g          311 t
ORIGIN      
        1 gccgccacct cggccaccac ctggtggccc ggcaccgtgg gaaagaccgg gggcggggtg
       61 cggccctcta tctcgtccag ttccgtgtga cacaccccgc aggccctcac cttgaggagt
      121 acctcgcccc cggccggctt cggctccggc aggtccatcg gctccagggg gttgtcggtc
      181 tttgccacgt cgcatatccc cgtgatggcg tacaccctca tcttcgtcct cctttgcccg
      241 gccacccggc ccgttctcct tcaccccgac cactgtccca cagagatgag cgtcatccct
      301 tctccactat aaccaccttc ccttccgatt gacacccctt gccgtgtgcg gttaaagtct
      361 ttctcacgga tgcagcttcc aaagggcatg atcatgaacg ggaggtgacg cgtgcacaga
      421 accagcgctt ctgaaaaagg taccgtaagc ccggccaccc tccgtgtcct cagcgtccgc
      481 ctgctcgtca tcctcgccct cctgccgatg gccgcgatat cgtgcagcag tgacggcggc
      541 ggaggcgggg gcgacggctc gagcgcggta cccgacgagc tctccttcag catggacctg
      601 agttccgact ttgtggaccc gttgaccatc acctcgtaca ccgattcaaa caagtccata
      661 accattgcga gcgtctctgg ccccgtctca ggcacctaca accacgcgac cggggagtac
      721 acgatcactg agggcgcctt ggtcaacgtg agatacagcc tctgggatga accgtacagg
      781 acgttcacca tctatctcgc ctccgacgta acaggctcga cggggcaaac cccccnnnnn
      841 nnnnncgggg ccctcgaggt ccgcagcggc accgataccg tgaccgtgac catctcgcca
      901 tcgggcgtcc gcctgtccct cgacggcgca gaccccacct cgacgatcgc ctggcaggat
      961 ctctacccca tgtactacga cgaagacgag ccgggctgga gaaaggaggc ctcgctcgcg
     1021 tgcgtggtcc tcaagttcct cgtcgaggag atcgcgttct ccgccgacac gctcgtcacc
     1081 atcgcgctga acgaggagac cctcgagaca gcgggatacc tcgaattcga gggagacccc
     1141 ttgccaccgt ccatcgcctt cggcgagagc acgagcgtgc tcaggctcac gagcccctcg
     1201 atcagggacg gggccacgtt ccagtggctc tatgactggt gctgggacga cgacccctcc
     1261 tcctccgaag acctcctcta cgacggcacg gtccagatga gcggctatac cgagacggtg
     1321 gacgtctcgg ggagcacccg ccggttcacc gagctcgggt tcggctccgg cacggccccg
     1381 ggcggggtgt cgtacgaccg cttcatgatc tacgtcaccg aggagacctc tggctcctac
     1441 accataccgg aggccaggac cctggtctac gacgggggct acagcatcct gttcacgagc
     1501 ggggactgag gccgggagaa ccgtgctgcc acgcgtcttc aggggacgac ggcgctgcgt
     1561 gggcggccgc ctgggcgccg cggccgcgcc ctcctttgcc cgggctctca aagccgccgt
     1621 cgggacgtgc aaaaaccggc gagtcgctcc cgcatccatg ctgcccatgg gcctgcacac
     1681 gatgacggct gcatcgatgc gggggtaagg ggactgcgcc ggcgccgccc ccaagcatgc
     1741 gggagacaca acggccgtat tgacaacctc ggccctttcg tctttatgag tgc
//
LOCUS       DAOSCR010000573         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44113_length_1787_cov_3.093533, whole genome shotgun sequence.
ACCESSION   DAOSCR010000573 DAOSCR010000000
VERSION     DAOSCR010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44113_length_1787_cov_3.093533"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLT69_11700"
     CDS             complement(<1..957)
                     /locus_tag="PLT69_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOJ15149.1"
                     /translation="MERHHKVSIWYVLIGIWLVLIAQNYIAQKIAVQTIPYSQFMTLL
                     KEGRITEIAVGQNRIQGKMKDKNGEVKEFKTIRVDPELSRMLDEYKVTFKGEIERTFA
                     RDLLSWVLPILVFVGIWYFIMKRMASQQPGFMSLGKNKAKIYVESELKVRFSDVAGVD
                     EAKQELVEVIEFLKNPDKFSELGGRIPKGILLVGPPGTGKTMLAKAVAGESGVPFFSM
                     SGSDFVEMFVGLGAARVRDLFEQAKQKAPCISFIDELDALGKARGIGLTAGHDEREQT
                     LNQLLVEMDGFDPKVGVILMAATNRPEILDPALLMPGRLVRHV"
     gene            complement(1245..>1787)
                     /locus_tag="PLT69_11705"
     CDS             complement(1245..>1787)
                     /locus_tag="PLT69_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15150.1"
                     /translation="PFPSTDLKIVDVETGTVEMKTGEAGEICFKGPQVMKGYYKRPDE
                     TEKVLRDGWLYTGDIGFLDEDGYLTIVDRKKDMIVASGYNIFPQEIDEILMSHPKVME
                     ACTIGVPDSYRGVAPKAFVVLKPGVTAEREELIAFLKERLAAYKVPKEIEFMDELPKS
                     AVGKILRKELRELEKKKGGA"
BASE COUNT          302 a          585 c          505 g          395 t
ORIGIN      
        1 gacgtgcctg acgagcctcc cgggcatgag cagcgccggg tcgaggatct cgggcctgtt
       61 cgtggcggcc atgaggatca cccccacctt ggggtcgaaa ccgtccatct ccacgaggag
      121 ctggttgagg gtctgctcgc gctcgtcatg accggcggtc aggcctatgc ccctggcctt
      181 gcccaaggca tcgagctcgt caatgaagct gatgcacggc gccttctgct tcgcctgctc
      241 gaagagatcc ctcaccctgg ccgcgcccag gcccacgaac atctcgacga agtcggaccc
      301 gctcatgctg aagaagggaa caccgctctc cccagcgacc gccttggcga gcatggtctt
      361 tcccgtgccg ggagggccca ccagcaggat ccccttgggg atgcggccgc cgagttcgct
      421 gaacttgtcg gggttcttga ggaactctat cacctccacg agttcctgct tggcctcgtc
      481 gacgcctgcg acatcggaga aacgaacctt gagctcgctc tccacgtaga tcttggcctt
      541 gttcttgccg agggacatga acccgggctg ctgggaggcc atcctcttca tgatgaagta
      601 ccagataccc acgaagacca agatcggcag gacccaggac aaaaggtccc gtgcgaatgt
      661 ccgctcgatt tcccccttga acgtcacctt gtactcatcc agcatcctcg agagctcggg
      721 atcgacacgg attgtcttga attccttcac ctcgccgttt ttgtccttca tctttccctg
      781 tatccggttc tggccgaccg cgatctccgt gatcctgcct tccttgagga gggtcatgaa
      841 ctggctgtag gggatcgtct ggaccgcgat cttctgcgct atgtagttct gggcgatgag
      901 cacgagccaa atgccgatga gtacgtacca gatggatacc ttgtgatgtc tctccacgcc
      961 ccctcctttc gacagacgtg cctgcaggat ttcttgatat acaagagtag tggattccaa
     1021 gcggaaatca accttcagga gaccttgggg gcggcccaag acggctcgcg gggcttcgcc
     1081 ttcgtcgtca gacatcccgg tggaggagac gacctcaagg ttcgcggaca acgctcgtgt
     1141 tcaggcccgg ccagggagac gacacggcgc ctctcggcgg tgtgcctttt ccctcacgcc
     1201 tcacggcagc ctgatcgatc cgggcgctcg atcccgtttg cgggctaggc accgcctttc
     1261 ttcttctcga gctccctgag ctccttccgg aggatcttgc ccacggcgct cttggggagc
     1321 tcgtccatga actcgatctc cttgggcacc ttgtaggctg ccagccgctc cttgaggaag
     1381 gcgatgagtt cctccctctc cgccgtcact cccggcttga ggacgacgaa cgccttgggc
     1441 gccacccccc ggtagctgtc cggcacacct atggtgcagg cctccatgac cttcgggtgg
     1501 ctcatgagga tctcgtcgat ttcctgtggg aagatgttgt agcccgaggc cacgatcatg
     1561 tccttcttcc tgtccacgat ggtgagatac ccgtcctcgt cgaggaaccc gatgtctccg
     1621 gtatacagcc agccgtccct gagcaccttc tcggtctcat cgggcctctt gtagtaaccc
     1681 ttcatgacct gagggccctt gaagcagatc tcgcccgcct cgccggtctt catctccacg
     1741 gtgcctgtct ccacgtcgac gatcttgagg tcggtgctcg ggaacgg
//
LOCUS       DAOSCR010000574         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44241_length_1783_cov_3.420718, whole genome shotgun sequence.
ACCESSION   DAOSCR010000574 DAOSCR010000000
VERSION     DAOSCR010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44241_length_1783_cov_3.420718"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLT69_11710"
     CDS             complement(<1..1288)
                     /locus_tag="PLT69_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOJ15151.1"
                     /translation="MAKKYVYLFGGRGTKAEGNAEMKDILGGKGANLAEMTNLGIPVP
                     PGFTISTEMCSVYYETGGLPKELKTQVKEAIKKVERAMGLGFGDPEAPLLFSVRSGAR
                     ASMPGMMDTVLNLGINDETVHGLIKMSGNERFAWDCYRRFIQMYGEVVMGADFDELEE
                     ELDKAKKKKGVKLDTELDADDLKKLVFVMKEKVKKLTGRDLPSDPMEQLWGGIEAVFR
                     SWNTPRAITYRKLNNIPDHWGTAVNVQAMVFGNMGDHSATGVAFTRDPSTGERIFYGE
                     YLRNAQGEDVVAGIRTPQPINIKGKRNKTDVSLEEAMPDQYAQLEEVYKTLEKHYRDM
                     QDIEFTIQEGNLWMLQTRNGKRTAAAAVKIAVDMVNEGLISKKEAVLRVSPEQIDQLL
                     HPQIDPKAEKKIIGKGLPASPGAAVGRVVFSAEDAEV"
BASE COUNT          299 a          591 c          529 g          364 t
ORIGIN      
        1 cgacctcggc gtcttcggcc gagaagacga cacggcccac cgccgccccg ggcgacgcag
       61 gcaggccctt ccctatgatc ttcttctccg ccttggggtc tatctgcgga tggaggagct
      121 ggtcgatctg ttcggggctc accctgagca ccgcctcctt cttggagatg agcccctcgt
      181 tcaccatgtc gacggcgatc ttgacggccg cggcggcggt gcgcttgccg ttgcgcgtct
      241 ggagcatcca gaggttgcct tcctgtatgg tgaactcgat gtcctgcatg tcgcggtagt
      301 gtttctcgag ggtcttgtac acctcctcca gctgggcgta ctggtcaggc atggcctctt
      361 ccagggacac gtcggtcttg ttcctcttgc ccttgatgtt gatgggctgg ggcgtcctga
      421 tgcctgcgac gacgtcttcg ccctgggcgt tcctgaggta ctccccgtag aatatcctct
      481 cgcccgtgga cgggtcgcgg gtgaaggcga cgcccgtggc cgagtgatca cccatgttcc
      541 cgaacaccat ggcctggacg ttcaccgctg tgccccagtg atcggggatg ttgttcagct
      601 tgcggtaggt gatggccctg ggggtgttcc acgacctgaa caccgcctcg atgccgcccc
      661 agagctgttc catcgggtca ctcggcaggt ccctgcccgt gagcttcttg accttttcct
      721 tcatcacaaa gacgagtttc ttgaggtcgt cggcatccag ctcggtgtcg agcttgaccc
      781 ccttcttctt cttggccttg tcgagctctt cctccagctc gtcgaagtcg gcgcccatga
      841 ccacctcgcc gtacatctgg atgaacctcc ggtagcagtc ccacgcgaac cgctcgttgc
      901 cgctcatctt gatgagcccg tgaaccgtct cgtcgttgat gccgaggttc aggaccgtgt
      961 ccatcatgcc gggcatggac gccctggcgc cagatcgaac ggagaagagg agcggcgcct
     1021 ccgggtcccc gaagcccagg cccatggccc gctcgacctt cttgatcgcc tccttcacct
     1081 gggtcttgag ttccttggga agcccgccgg tctcgtagta gacggaacac atttccgtgg
     1141 agatcgtgaa gccgggcggc accgggatgc cgagattggt catctcggcc aggttcgcac
     1201 ccttgccacc caggatgtcc ttcatctccg cattgccctc ggccttggtg ccccttcccc
     1261 cgaacagata cacgtacttt ttcgccatga tcatctccct ctcacgtaac tggtcagaaa
     1321 ccggaactca gcttgaagcg cgcaaaagat agtatacaga aagggttatc atcaagagaa
     1381 aaatgcccct gcccacgggc ctgcctgcgc cttgccagcg ctgcccgcag gcgtccgaga
     1441 cgggcgaact gcaccgagcg tccacgcacg aaacgaccgt gtcaccccgg gccgtgcagg
     1501 catccggcag gacgtgggga tgagagactt caccggccgc attgaactgc cgggaaaagc
     1561 cgtgcaggca tcagtcagga cgcggggatt caagacggcg ggtgctccac ggtgcggggt
     1621 cgaggtttga caggggcgca cgatcgcccg caccggtaca gccccaagac ctcccgggcc
     1681 tgaaagcggc tccgggacaa aggcagaccg ccgggacaca cacgcacctc acacgtcgcc
     1741 ccctgtgcgt cgagctgaac gtgcctcccc gccaatgaag gaa
//
LOCUS       DAOSCR010000575         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44475_length_1777_cov_3.654472, whole genome shotgun sequence.
ACCESSION   DAOSCR010000575 DAOSCR010000000
VERSION     DAOSCR010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44475_length_1777_cov_3.654472"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLT69_11715"
     CDS             <1..389
                     /locus_tag="PLT69_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15152.1"
                     /translation="MVGWNIEIMFMFSLVGIIFYHTLTEGRKVKILGLPEMWFWAIAY
                     SAFCVFVECLLNIGGHLVWEYPFWNLSLKGVPLIFLIGYFHFFCAAIWVITRKTMKAK
                     IALLVLFYSVPAVMNILAFGVFGWNY"
     gene            complement(769..>1777)
                     /locus_tag="PLT69_11720"
     CDS             complement(769..>1777)
                     /locus_tag="PLT69_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOJ15153.1"
                     /translation="ALLNIAGPGDEIVALDNLYGGTYNLFRHTLSRLGLNVKFVPSED
                     PGALEKAIGPRTKAVYAESIGNPKLDVTDLELVSLIAHGFGVPFVLDNTVTPYLLRPF
                     DHGVDIVVYSATKFIGGHGTSMGGVIVDSGRFDWSRGDFPLITEADPGYHGTVFPETF
                     GNMAYIVKARVDLLRDLGCAMSPFNAFLFIQGLETLHLRMIRHSENALEVARYLERHP
                     SVAWVNYPGLESSPQNEKAKRYLPMGAGAIVGFGIRGGMEAGRRFIDALKLISHVANI
                     GDVRTLAIHPASTTHQQLTREERASTGVTDDFIRLSVGIEHIDDILADIEQALASAGS
                     A"
BASE COUNT          292 a          579 c          560 g          346 t
ORIGIN      
        1 ccatggtggg ctggaacata gagatcatgt tcatgttctc cctcgtgggc atcatcttct
       61 accacaccct caccgagggc aggaaggtga agatcctggg actcccggag atgtggttct
      121 gggcgatcgc ttactcggcc ttctgcgtgt tcgtcgagtg cctcctcaac atcggcggtc
      181 acctggtgtg ggaatacccc ttctggaacc tctccctcaa gggcgtgccc ctcatcttcc
      241 tcataggcta cttccacttc ttctgcgccg cgatatgggt catcacgcga aagaccatga
      301 aggccaagat cgccctcctg gtgctgttct acagcgtacc cgcggtcatg aacatcctgg
      361 ccttcggcgt gttcgggtgg aactactgac agggcacggt acccaaagac accctgtcct
      421 gccttggggg gcgcgatcct cgccccccgc ccaccgggcc tcggtgcccg ccagctggtg
      481 ggccgtctct caacgcgccg ggggctcacc acggcacctc gagggtcggg gacacgggga
      541 gggctcgttg ctcttcacgg cgccgaacgg gcaccgcacg aggggtcgcg ggccgagggc
      601 gggcgtgtga cgcaccgggc gacgtccatg catgcgtcag ggggcacggg cttgtcgtcg
      661 gccgccaagc ccccttttcg cggaccgggc acagctctcc cccggtttcc ctgcggggat
      721 cgcccgtccc cgcggacccg gagttcaggg agaggggacc ggcatgggtc atgccgaccc
      781 ggccgaggcc aatgcctgct ctatgtccgc aaggatatcg tctatgtgct cgatccccac
      841 ggaaagcctt atgaagtcgt cagtgacccc ggtcgaggcc ctctcctctc tcgtgagctg
      901 ctggtgggtg gtgcttgccg ggtggatggc cagtgtcctc acatccccta tgttcgcgac
      961 atgggatatg agcttcagcg catcgatgaa tctccggccc gcttccatcc ccccccggat
     1021 gccgaacccc acgatggccc ccgcgcccat gggcaggtat ctcttcgcct tttcgttctg
     1081 cgggctcgac tcgagcccgg ggtaattcac ccaggccacc gacgggtgcc gctcgaggta
     1141 cctcgcgact tcaagggcgt tctccgagtg cctgatcatg cgcaggtgta gggtttcgag
     1201 gccctggata aagaggaagg cattgaacgg gctcatcgcg cagccgaggt ccctcaggag
     1261 atcgacccgc gccttcacga tgtacgccat gttcccgaag gtctcgggga agaccgtccc
     1321 atggtagccg gggtcagctt cggtgatgag cgggaagtcc ccccgggacc agtcgaacct
     1381 ccccgaatcc acgatgacac cccccatcga ggtgccgtgg ccgccgatga acttggtcgc
     1441 ggaatacacc acgatgtcca cgccgtgatc gaatggcctc aacaggtacg gggtcaccgt
     1501 gttgtcgagc acgaagggga ctccaaagcc gtgcgcgatc agggaaacga gctcgaggtc
     1561 ggtcacgtcg agcttggggt tccctatgga ctcggcgtag accgccttcg tcctcggtcc
     1621 tatggctttt tcgagcgcgc ccgggtcttc ggaggggacg aacttcacgt ttaagcccag
     1681 gcggctcaac gtgtgacgga agaggttgta ggtccccccg tagaggttgt cgagcgccac
     1741 gatctcgtct ccaggcccgg cgatgttcag gagggcg
//
LOCUS       DAOSCR010000576         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44480_length_1777_cov_3.388502, whole genome shotgun sequence.
ACCESSION   DAOSCR010000576 DAOSCR010000000
VERSION     DAOSCR010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44480_length_1777_cov_3.388502"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1777)
                     /locus_tag="PLT69_11725"
     CDS             complement(<1..>1777)
                     /locus_tag="PLT69_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15154.1"
                     /translation="FLPREEREAFDNVLRAVVGTGRPVVHETRLTAKNGGVLVVEWRS
                     CAVFKPDGTPEFVFGIGIDVTEKRKAEEDLAERRRQLETLVDNLPGMAYRCKNDRDWT
                     MDFVGSASLGLTGYTPEELVHNKAVSYADLIHPDDREKVWEEVQRSLARRERFQITYR
                     IVTKDGRTLKVWEQGQGVFDAEGNVQSIEGFIMDVTEVEEAQEELRKSEERYRLLARN
                     VQDVIWVLDLDLNHTYVSPSVERMRGFTVEEVLRQRMDEVLTPESYAYATRVFVEELE
                     RERSGVQREAHWSRTIELELWKKDGSTLWTEVTASPLRDDTGEVIGVVGITRDISERK
                     KLEEAIAQVQFAVEHIAECAFWVDGEGRIIYVNEAACASLGYTKDELTRMHMPDIHPR
                     SGGDSWDRRWEDLKRQQRLSYETFLLRKDGGVFPVEINANLLTFRGRQFCWAFARDIT
                     ERKEAERALRESEERFRRMAEAIPMMFFMVTPSFDGLIYVNPACRNILGMGEDALRGN
                     PRAWMDLVHPDDRAFVRKAVNEDAERDAALEFRIERPDGSWGWIHMRLTPVFDAQGAP
                     SCRVGTAEDITRRKEMEHERELAQAH"
BASE COUNT          255 a          627 c          515 g          380 t
ORIGIN      
        1 ggtgggcctg tgcgagctct cgctcgtgtt ccatctcctt gcggcgggtg atgtcctccg
       61 ccgtgcccac gcggcaggag ggcgcgccct gcgcgtcgaa gaccggggtg agcctcatgt
      121 ggatccatcc ccaggagccg tccggtcttt cgatcctgaa ctcgagggcg gcatcccttt
      181 ccgcatcctc gttcacggcc ttccgcacga acgccctgtc gtccgggtgc acgagatcca
      241 tccaggccct cgggttccca cggagagcgt cttcccccat ccccaagata ttcctgcatg
      301 ccgggttcac gtagatgagg ccgtcgaatg acggggttac catgaagaac atcatgggga
      361 tggcctccgc catcctcctg aagcgctcct cgctctccct gagggccctc tcggcctcct
      421 ttctctccgt gatgtccctg gcgaaggccc agcagaactg tctgcccctg aacgtgagga
      481 ggttcgcgtt gatctccacg gggaatacgc ctccgtcctt cctgaggagg aacgtctcgt
      541 aactcagtct ctgctggcgc ttcaagtctt cccacctgcg atcccacgag tccccgccgg
      601 agcgcgggtg gatgtcgggc atgtgcatgc gtgtgagctc gtccttggtg tatccgagcg
      661 aagcgcaggc cgcctcgttc acgtagatga tgcggccttc gccgtcgacc cagaaggcgc
      721 actccgcaat gtgctccacc gcgaactgga cctgggctat ggcctcctcg agcttcttgc
      781 gctcggagat gtccctggtg atgcccacga cgccgatgac ctccccggtg tcgtccctca
      841 aggggctcgc cgtgacctcg gtccacaggg tggagccgtc ctttttccag agctcgagct
      901 cgatggtcct cgaccagtgc gcctccctct ggacgccgga ccgttcgcgt tcgagctcct
      961 ccacgaagac cctcgtagcg tacgcgtagg actcgggggt gagcacctcg tccatcctct
     1021 gcctgaggac ctcctccacg gtgaatcccc tcatcctctc gacggacggg ctcacgtagg
     1081 tatggttgag gtcgaggtcg agcacccaga tcacgtcctg gacgttcctg gcgaggaggc
     1141 ggtagcgctc ctcgctcttt ctgagctcct cctgggcctc ttccacctcg gtcacgtcca
     1201 tgatgaaccc ctcgatggac tggacgttcc cctcggcgtc gaacacaccc tggccctgtt
     1261 cccatacctt gagggtcctg ccgtctttcg tgacgatcct gtaggtgatc tggaagcgct
     1321 cccgccgagc tagcgagcgc tgcacctcct cccagacctt ttccctgtcg tcagggtgta
     1381 tgaggtcggc ataggacacc gccttgttgt gcacgagttc ttccggggtg taccccgtca
     1441 ggccgagcga ggcgctcccc acgaagtcca tggtccagtc acggtcgttc ttgcagcggt
     1501 acgccatgcc cggcaggttg tcgacgagcg tctccagctg cctgcgtcgc tcggcgaggt
     1561 cctcctcggc ctttctcttt tccgtgacgt cgatgccgat gccgaagacg aactcgggtg
     1621 tcccgtcggg cttgaatacc gcgcagctcc tccactccac gacgaggacc ccgccgttct
     1681 tcgcggtgag cctcgtttcg tgcacgaccg gcctgcccgt gcccaccacc gccctcagca
     1741 cgttgtcgaa cgcctcccgt tcctccctcg ggaggaa
//
LOCUS       DAOSCR010000577         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44599_length_1774_cov_3.228621, whole genome shotgun sequence.
ACCESSION   DAOSCR010000577 DAOSCR010000000
VERSION     DAOSCR010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44599_length_1774_cov_3.228621"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLT69_11730"
     CDS             complement(<1..715)
                     /locus_tag="PLT69_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15155.1"
                     /translation="MGDRKFTEVASALCSLAGTEQARGLLRTFSFQLGWRPALGILAK
                     TACRTGPLRTIRDLAAFRLSEITPRFITSMWETFGEREILVSEGRRFTYSHFKNRVFR
                     LANALVSLGLRPGDRVAELLYNGNEFFEAFFACSFIGCPMPFLNWHLHGEELAEAIGR
                     ARPKALILHEEFLDEIEAVKDRLESVEHFIVVGPRVPGGMNSYEGLMAGSDETMPGCR
                     LVVAMNPYTGGSTGTPKNVN"
     gene            complement(1173..>1774)
                     /locus_tag="PLT69_11735"
     CDS             complement(1173..>1774)
                     /locus_tag="PLT69_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15156.1"
                     /translation="KSDIVIKTGIPIPLVDARIVDKDGTFLPTDGESTGEIVVRAPWF
                     THGYFKDPAQSGELWRGGWLHTGDVGHMDAEGYLQITDRLKDVIKTGGEWISSLDLEN
                     AISSHEAVLEAAAVGVPDAKWGERPVLLVALKPPYRNNGVTEDALREFMRQCAEQGRI
                     PRYGVPDRCIITEDIPKTSVGKIDKKLIRSMLAEKGASS"
BASE COUNT          286 a          573 c          590 g          325 t
ORIGIN      
        1 agttcacgtt cttgggcgtg cccgtggatc cgcccgtgta cgggttcatg gccaccacga
       61 gcctgcatcc gggcatggtc tcatccgagc cggccatgag cccctcgtac gagttcatgc
      121 ccccggggac acgcgggccc accacgatga agtgctccac gctttccagc ctgtccttca
      181 cggcctcgat ctcgtccagg aactcctcgt gcaggatcaa cgccttgggc ctggccctgc
      241 cgatcgcctc ggcgagctcc tcgccgtgga ggtgccagtt cagaaacggc atggggcagc
      301 cgatgaagga gcaggcgaag aaggcctcga agaactcgtt gccgttgtag agcagctccg
      361 ctacgcggtc gccgggcctc aaccccaggg agacgagggc gttggcgagc ctgaagacgc
      421 ggttcttgaa gtgggagtag gtgaacctcc gcccttctga gacgaggatc tccctttcgc
      481 cgaaggtctc ccacatgctc gtgatgaagc ggggtgtgat ctcggacagc ctgaaggcgg
      541 ccaggtcgcg gatcgtccga agcgggccgg tcctgcaggc ggtcttggcg aggatgccca
      601 gggcggggcg ccacccgagc tggaaggaga acgtcctgag caggccgcgg gcctgctccg
      661 tgcccgcgag cgaacagagg gccgaggcga cctcggtgaa cttcctgtca cccatgcgaa
      721 acctcccctg agacagtgcg tcttcggttg tccgaaccct cagccagcgg cgcgtacccg
      781 ccgatggagg ccccttcggc ccgcggtacc gatgccgcca tgacaagtca tatgaaaaga
      841 ttataataca acaataccgg ttccgggaca agggagagac ccgggcaccc tccgcccagg
      901 tcggtctctt caggatggca cgcggggaga acgggcgccg ggtggtcttg gcgggttgga
      961 cgacaagccg agggctttct cacgcggcgt gagacggcct cgcacggtgt gggacgtgtc
     1021 ctcggggcct gcgcagggag cttgtggcgc gaggagctct tcgtgccctc gcacgcggcg
     1081 aagaaagatg cgggcccgca aaggggcccg ctgaaaccac cccggagttg tcaggtggtg
     1141 tcgggaagac ggggataggt tcgtgcgcct cgttacgagg aggcgccttt ttccgcgagc
     1201 atggacctga tgagtttctt gtcgatcttg cccacgctcg tcttggggat gtcctccgtg
     1261 atgatgcacc tgtccggaac gccgtacctg ggtatcctgc cctgctccgc gcactgccgc
     1321 atgaattccc tgagcgcgtc ctcggtgacc ccgttgttcc ggtacggtgg tttcagtgcg
     1381 accagcagaa cgggccgctc gccccacttc gcgtccggga ccccgacggc ggcggcctcc
     1441 agcaccgcct cgtgcgagct gatggcgttc tcgagatcga gggacgagat ccactctccc
     1501 cctgtcttta tgacgtcctt gagacggtcc gtgatctgga ggtacccctc ggcgtccatg
     1561 tggcccacgt cccccgtgtg cagccacccg ccgcgccaga gctcgccgct ctgggctggg
     1621 tccttgaagt agccgtgggt gaaccacggc gccctcacga cgatctcacc ggtgctctcg
     1681 ccgtcggtcg gcaggaacgt gccgtctttg tccacgatcc gcgcgtcgac gaggggtatg
     1741 gggatgccgg tcttgatgac gatgtcgctc ttcg
//
LOCUS       DAOSCR010000578         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44613_length_1774_cov_2.011053, whole genome shotgun sequence.
ACCESSION   DAOSCR010000578 DAOSCR010000000
VERSION     DAOSCR010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44613_length_1774_cov_2.011053"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..782)
                     /locus_tag="PLT69_11740"
     CDS             complement(294..782)
                     /locus_tag="PLT69_11740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOJ15157.1"
                     /translation="MGGDATLPQESTPAKGSGDGCVFCKIVSGAAPAYKVYEDETTLC
                     FLDIHPYTKGHCLVIPKRHVTWWHEMTEEETASVFKTARMCARKMVKAFGCDFVFLYA
                     RGRRIPHTHIFLIPTHKDDVLDRFFHALEKFQESPSELARLKDANDMVQAMEILRETP
                     GE"
     gene            complement(1165..>1774)
                     /locus_tag="PLT69_11745"
     CDS             complement(1165..>1774)
                     /locus_tag="PLT69_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15158.1"
                     /translation="GCGMNQDTLERIFDPYFTTKPKGEGTGLGLAIVHTIVRDMGGAI
                     RVSSIPGKGSSFHVYLPSHTGCVREKVFPTKAPTGGNEHVLVVDDERAIVDITSQMLK
                     RLGYRVTAFMDSVEALEHFRRSKEGYDVVITDLTMPKMNGDLLAEQIVSIRPDIPVIL
                     CTGYSSEVSRERVLRAGVTEVLAKPVSLDLLAQTVRNVLDGR"
BASE COUNT          300 a          609 c          532 g          333 t
ORIGIN      
        1 ctctgagggg gatgaaacgt catgcgtcca cccatgatca gaggtggctg tacggtatcc
       61 cgtgctgtgg acgatggcgg ccggcgcccg gatgagccgg gctcgaccag gcgtcatcga
      121 gaacaccacc tctgtgcagg tccgcccatc ccggcccggg acatcgtcat gggcagggcg
      181 acggctcagg cgaccctggg gaggcggcgg tgcgacgcga tcgccgctca ccagccttca
      241 gccaggcatc acggcctagc tcttcgacga cgccggcccg cctcgccttg acgtcactct
      301 ccaggcgtct ccctgaggat ctccatggcc tggaccatgt cgtttgcgtc cttgagccta
      361 gccagctccg acggtgattc ctggaacttt tccagggcgt ggaagaagcg gtcgagcacg
      421 tcgtccttgt gggtgggtat gaggaagatg tgcgtgtggg ggatgcggcg cccccgggcg
      481 tacaggaaca cgaaatcaca cccgaaggcc ttcaccatct tccgggcgca catccgcgcc
      541 gtcttgaaca cggaggccgt ctcctcttcg gtcatctcgt gccaccaggt gacgtgacgc
      601 ttgggtatga ccaggcagtg acccttcgtg tacgggtgga tgtccaggaa gcacaaggtg
      661 gtctcgtcct cgtacacctt gtacgccggt gcagccccac tgacgatctt gcagaacacg
      721 cacccgtccc cggagccctt cgcaggcgta gactcctggg gcaatgtcgc atctcctccc
      781 atcccggacc tccttcctcg tactcgaaac acgacctggc agacacgggc ggaatatacc
      841 ccaccgcacg atccttgaca attcccatgc catacggacc gagaggccga tgccgcaggg
      901 acactccggc catccccacg cggcgtcggc cgacgactcc tcgcgggctc aacagtgccg
      961 catgatcctt tctccaacga ttggacaggc cctgggttct tccaggtgag ctttcagcca
     1021 gcgcctacca ccaccccagg accggcgacg catgacttcg cggagaccgg gcaggcacct
     1081 ccaccctccc tcgttccttc tggaagatac cggggccggt cgcacccgcc tcagcggacc
     1141 ttcgccggac gagcatgggg gctttcacct tccgtcgagg acgttccgca cggtctgggc
     1201 gagcaggtcg aggctcaccg gcttggccag cacctcggtc acccccgccc tgagaacccg
     1261 ctccctggag acctcgctgg agtatcccgt acagaggatg accggtatgt cgggcctgat
     1321 gctgacgatc tgctctgcga ggaggtcgcc gttcatcttc ggcatggtga ggtccgtgat
     1381 caccacgtca tagccctctt tgctgcgcct gaagtgctcg agggcctcca cgctgtccat
     1441 gaaggcggtc acccggtagc cgagcctctt cagcatctgg gaggttatgt ccacaatggc
     1501 ccgctcgtcg tccaccacga gcacgtgctc gttcccgccg gtcggggcct ttgtgggaaa
     1561 gaccttctct ctcacacaac cggtgtgcga ggggaggtac acgtggaagg acgacccctt
     1621 gcccgggatg ctgctcaccc gtatggcccc tcccatgtcc ctgacgatcg tgtgcacgat
     1681 cgcgagcccg agcccggtcc cctcaccctt gggcttggtg gtgaaatagg ggtcgaagat
     1741 cctctcgagg gtatcctggt tcatcccgca accc
//
LOCUS       DAOSCR010000579         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44912_length_1765_cov_2.937427, whole genome shotgun sequence.
ACCESSION   DAOSCR010000579 DAOSCR010000000
VERSION     DAOSCR010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44912_length_1765_cov_2.937427"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1282)
                     /locus_tag="PLT69_11750"
     CDS             complement(122..1282)
                     /locus_tag="PLT69_11750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ15159.1"
                     /translation="MPVAARMLDFMAKASWIRKMFEEGARLKSIHGADKVYDFSLGNP
                     DVPPPPRVIESMSALARESFHGYMPNAGYPEVRNVLAAHLGGIYGVALTGEEIVMTCG
                     AGGAMNVVLKALLDPGDEVIALAPYFVEYGFYADNHGGRLVTVPCRDDFLPDIRAIER
                     AIGPSTKAIIVNSPNNPTGRVYPESVIEDLARLVRSYPHVAVISDEPYRAIVYDAREV
                     PSILAYIPNSIVVSSASKELSLAGERIGYIAVNPSMDRKDDLVAAMILANRILGFVNA
                     PALMQRVIAACVRERVDTGIYERRRNTMTSVMDDTGLTYAPPEGAFYLFVRSPLSDEV
                     AFCNLLLEERVLAVPGRGFGWPGWVRFAYCVNETVILNAHDGILRAAKRAGV"
     gene            1553..>1765
                     /locus_tag="PLT69_11755"
     CDS             1553..>1765
                     /locus_tag="PLT69_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15160.1"
                     /translation="MTGRIRSPYIISVLLAVMVGVIYYNTLDNSPTNWDDPVLFTRTA
                     YRGFTLEHVKDVLTFHEGSTYQPLRDL"
BASE COUNT          299 a          566 c          591 g          309 t
ORIGIN      
        1 cgctccggtg gcgccgagca tcgagacctt cgccgacgac gtgcccttct gatctcgaga
       61 cacgggccgg gcagttcgtc cagggcggct cgatgcggcc tttcccgggc cgcacccagg
      121 ctcagacccc tgcccgcttg gccgccctga ggatgccgtc atgggcgttc aagatcacgg
      181 tctcgttgac gcagtaggcg aacctcaccc agccgggcca gccgaacccc ctgccgggga
      241 cggcgagcac gcgctcctcg agcaggaggt tgcagaacgc cacctcgtcc gacaggggcg
      301 agcggacgaa gagatagaac gcgccctcag gcggggcgta ggtcaggccc gtgtcgtcca
      361 tgacgctcgt catggtgttc ctacgccttt cgtagatccc cgtgtccacc cgctccctga
      421 cgcacgcagc gatcacccgc tgcatgaggg cgggcgcgtt gacgaacccg aggatgcggt
      481 tcgcgaggat catggcggcc acgagatcgt ccttgcggtc catggagggg ttgacggcga
      541 tgtacccgat gcgttcgccc gcgagggaga gctccttgga ggccgaggac accacgatgg
      601 agttgggtat gtacgcgagg atcgaaggca cctccctggc gtcgtagacg atggccctgt
      661 acggttcgtc agagatcacc gccacgtggg ggtatgatct cacgagcctt gccaggtcct
      721 cgatgacgga ctcggggtag accctgcccg tggggttgtt cggcgagttc acgatgatcg
      781 ccttggtgga gggaccgatt gcgcgctcga tcgcccgtat gtcagggagg aagtcgtcgc
      841 ggcagggaac cgttacgagg cgcccgccgt ggttgtccgc gtagaaaccg tactccacga
      901 agtaaggcgc cagggcgatc acctcgtcac cggggtcgag gagcgccttg agcaccacgt
      961 tcatggcgcc gccggccccg catgtcatga cgatctcctc ccccgtgaga gcgaccccgt
     1021 agatcccgcc caggtgtgcg gcgagtacgt tgcgcacctc ggggtagccc gcgttcggca
     1081 tgtacccgtg gaaggactcc cttgcgaggg cagacatgct ctcgatgacc cggggcggag
     1141 gcggcacgtc cgggttgccc aggctgaagt cgtacacctt gtctgcgccg tggatggact
     1201 tgaggcgcgc tccctcctcg aacatcttcc gtatccagga agccttggcc atgaaatcga
     1261 gcattcgagc agccaccggc atggtacacc ctcctcgcca ttgaatggtg tgcatgatac
     1321 cagcccgtct tcccgatgta cagccgaggc ggaggggtgg ggccgggatg ctcccgggtg
     1381 tctgcagccc cggcgaaggg cgcgcgacgg acggctgggt gtgggcgccg gccgcacgca
     1441 ccctttctgc cggggtattg cgtattgtcg atgcagtccg gggatgctat gtggggttca
     1501 tcatagaggg catggggcgc gacgaatcgc cccggcctgg aaaggaccgg acatgacagg
     1561 gaggatccgc tcgccttaca tcatctccgt gcttctcgcc gtgatggtcg gggtgatcta
     1621 ctacaacacc ctcgacaaca gcccgaccaa ctgggacgac ccggtcctct tcaccaggac
     1681 ggcataccgg ggattcacct tggagcacgt caaggacgtg ctgaccttcc acgagggctc
     1741 cacgtaccag ccgctgcgtg acctg
//
LOCUS       DAOSCR010000580         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44913_length_1765_cov_2.909942, whole genome shotgun sequence.
ACCESSION   DAOSCR010000580 DAOSCR010000000
VERSION     DAOSCR010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44913_length_1765_cov_2.909942"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLT69_11760"
     CDS             complement(<1..221)
                     /locus_tag="PLT69_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15161.1"
                     /translation="MARFEEKSIPAVFQNRAEKFGERAICAFKNARGVYEDISWNKMN
                     TIVRNLGYFLIAHGIRPGDKVALFSPNRW"
     gene            550..843
                     /gene="gatC"
                     /locus_tag="PLT69_11765"
     CDS             550..843
                     /gene="gatC"
                     /locus_tag="PLT69_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOJ15162.1"
                     /translation="MRITEQEVLHVAHLARLSIGGEEAARFRRELGSILEYMDMLSEV
                     DTSGVEPFVCVHEQASATRDDEPAETLPLRLVLAQSPAAGHGAFEVPLVIEEG"
     gene            847..>1765
                     /locus_tag="PLT69_11770"
     CDS             847..>1765
                     /locus_tag="PLT69_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOJ15163.1"
                     /translation="MLDKARRFRTEDLSPVDELERLLERCRASDLNAYITLNGDEALD
                     AAEQALQRLDEGDDDPLVGLAIAVKDNICTKGLRTTCASRMLADYVPPYDAHVIRLLR
                     RKNAVILGKTNMDEFAMGSTTVTSAFGPARNPWDPMRTPGGSSGGSAAAVAAGLCDAA
                     LGSDTGGSIRQPASFCGITGLKPTYGSVSRYGLVAFASSLDQIGPMARSARDVAVLFD
                     AIRGHDPRDSTSAEAGCPPIPLEGMDLRGITIGVCRSFFSEGLDRDVERSVRHGLAVF
                     EDLGAKVVDMDLPHLRYSVAAYYLIAPAEA"
BASE COUNT          314 a          558 c          593 g          300 t
ORIGIN      
        1 tcccagcggt tgggggagaa gagcgcgacc ttgtcaccgg gtcgtatccc gtgcgcgatg
       61 aggaaatacc cgaggttgcg cacaatggtg ttcatcttgt tccacgagat gtcttcgtag
      121 acccccctcg cattcttgaa cgcgcagatg gcgcgctcgc cgaacttctc ggcccggttc
      181 tggaatacgg ccgggatgga tttctcctcg aaccttgcca tgatgccctc cttccttgat
      241 cattctgtgg tctgaggcaa aacgcaatga atataaagca gtatacgaga aacattgccg
      301 gagatcaaac caaaaattga cgaggataaa tcaacggcag gctctccttg aggacggctg
      361 agtattggta taatgaatga aagttccttg gtgccgtgcg gggccgaagg tgcggggcgt
      421 catcgcggca agtccgccgt gacatgcccc tcggagagct ggggaagtgc gtcgggcggg
      481 cgcgggcggc ccgggcgttt gttgccgggc tccattccta gctggcaaac gcgacgggag
      541 gtgagagggg tgaggatcac cgagcaggag gtgctccacg tggcgcacct cgcccgtctc
      601 tcgatcgggg gcgaagaggc ggcgaggttc cggagggagc tgggttcgat actggagtac
      661 atggacatgc tctcggaggt cgatacatcc ggggtggagc ccttcgtctg cgtgcacgag
      721 caggcgtccg cgacgaggga tgacgagccc gcagagacgc tccctctccg gctcgtgctc
      781 gcccagtcgc ctgcggcggg tcacggcgcc ttcgaggtgc ctctcgtcat cgaagagggg
      841 tgacggatgc tcgacaaggc ccggaggttc cgcaccgaag acctttcccc ggtggacgag
      901 ctcgagcgcc tgctcgagcg ctgccgcgcg agcgacctga acgcgtacat caccttgaac
      961 ggcgacgagg ccctggatgc ggccgagcag gccttgcaga ggctcgacga gggcgacgac
     1021 gacccgctcg tgggcctcgc catagcggtc aaggacaaca tctgcacgaa gggcctgcgc
     1081 accacctgcg cctcccggat gctcgccgac tacgtgccgc cctacgacgc ccacgtgata
     1141 cggctcctca ggcgcaaaaa cgcggtgatc ctcggcaaga cgaacatgga cgagttcgcc
     1201 atggggtcga cgaccgtaac gagcgcgttc ggcccggcca ggaacccgtg ggaccccatg
     1261 cgcacccccg gcgggtcgag cggcgggtcg gccgccgcgg tcgccgccgg gctctgcgac
     1321 gctgcgctcg gatcggacac cggcgggtcc atccgccagc ccgcgagctt ctgcggcata
     1381 accggcctga agcccaccta tggttccgta tcccgctacg gcctcgtcgc cttcgcctca
     1441 tcgctcgacc agatcggccc catggcgagg agcgcgcgtg acgtggccgt gctcttcgac
     1501 gccataaggg ggcacgatcc gagagactcg acgagcgcgg aggcgggttg ccctcctatc
     1561 ccgctcgaag ggatggacct ccgtgggatc accatagggg tctgcaggtc cttcttctcc
     1621 gaggggctcg acagggacgt ggagagatcg gtgaggcacg gcctcgccgt gttcgaagac
     1681 ctcggggcga aggtcgtcga catggacctg ccgcacctca ggtattccgt cgccgcctac
     1741 tacctcatcg cgccggccga ggcca
//
LOCUS       DAOSCR010000581         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_44942_length_1764_cov_2.616150, whole genome shotgun sequence.
ACCESSION   DAOSCR010000581 DAOSCR010000000
VERSION     DAOSCR010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44942_length_1764_cov_2.616150"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..910)
                     /locus_tag="PLT69_11775"
     CDS             complement(143..910)
                     /locus_tag="PLT69_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOJ15164.1"
                     /translation="MDAVLSLETLASLVTLASIEIVLGVDNIVFLSIMVEKLPAQRRK
                     SARVFGLGLAMVTRILLLFFLSAMMRLTRPLFSLAGHAFSARDLILLGGGIFLLAKAT
                     FEIHENLEGASPHAEPKGAGAGFAAVVLQIAVLDIVFSLDSVITAIGLARELWIMVAA
                     IVIAVLFMMFLSGAVILFVERHPTIKMLALSFLLLIGVALVADGLSVHIPRGYIYFAM
                     AFSVFVEVLNLKLMKKKAPEPVRLRRLEGVSVDEDTP"
     gene            complement(924..1610)
                     /locus_tag="PLT69_11780"
     CDS             complement(924..1610)
                     /locus_tag="PLT69_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOJ15165.1"
                     /translation="MAADEYVFVPGQYGEKITTLVSMPDDYQSGTKPAVILAHGAGND
                     MFHPLLVHMAHLLENSGYLTVRFNFPFRDRGRRNPDSDEALMASWMSVFSYFRDHPLY
                     QCARIVAAGKSLGGRVALQASAAGLIDPTGLVFFGYPLHPPGKKDILRDAPLHRVQAP
                     MLFFAGSSDPFCDTGILRDVLGRVNAPWSLEVIEGADHSFKVPKDSALTGEQVFARIL
                     GVTLHWLENL"
BASE COUNT          338 a          565 c          570 g          291 t
ORIGIN      
        1 cacctcgtac catgccggtc accctctcct ggctgtcccc aagcgccgat ccaccgcgcg
       61 gccatgccgc ccacacgggc cgggtgctga tcgccgtcat cgaggccgac ggagaagacc
      121 ccccccttcc cacagcggcc gttcaggggg tgtcttcgtc cacggacacg ccctcgagtc
      181 tcctgagtct taccggttcc ggggcctttt tcttcatcag cttcagattg aggacctcca
      241 cgaacaccga gaacgccatg gcgaagtaga tataaccgcg gggtatgtga accgagagcc
      301 cgtcggcgac gagcgcgacc ccgatgagca gcaggaagct cagggccagc atcttgatgg
      361 tgggatgccg ttccacgaac agtattaccg caccggagag gaacatcatg aaaaggacgg
      421 cgatcacgat cgccgcaacc atgatccaga gctcgcgcgc aagccctatg gccgtgatca
      481 ccgagtcgag ggaaaagacg atatcgagca ctgcgatctg caggacgacc gctgcgaaac
      541 cggcccccgc ccctttcggc tcggcatgcg gggatgcccc ctccaggttc tcgtggatct
      601 cgaaggtggc cttcgcgagc aggaagatgc cgccgcccaa caggatcagg tcacgggcgg
      661 aaaaggcatg cccggcaagc gagaagaggg gcctggtgag cctcatcatg gcgctcagga
      721 agaagaggag gagtatccgc gtgaccatgg cgaggccgag accgaagacg cgggcgctct
      781 tgcgcctctg ggcagggagc ttctcgacca tgatcgagag gaagacgatg ttgtctacgc
      841 cgaggacgat ctcgatggag gcgagcgtga cgagcgacgc cagcgtctcg aggctcagga
      901 cggcatccat ggcggcgctc cccttacagg ttctcgagcc agtgcagggt gaccccaagg
      961 atgcgcgcga agacctgttc cccggtgagc gccgagtcct tcggcacctt gaaggagtgg
     1021 tcggcccctt cgatgacctc gaggctccac ggggcgttca cgcgcccgag cacgtctctc
     1081 agtatgcccg tgtcgcagaa cgggtcggag gaaccggcga agaagagcat gggcgcctgc
     1141 acgcggtgca ggggcgcatc cctgaggata tccttctttc cgggcgggtg cagggggtac
     1201 ccgaagaaga cgaggcccgt cgggtctatg aggcctgccg cggacgcctg gagcgcgacc
     1261 ctgccgccca gggacttgcc tgcagcaacg atgcgcgcgc actggtagag cgggtgatcc
     1321 ctgaagtagg agaacacgct catccatgat gccatgagcg cctcgtcgct gtcggggttc
     1381 ctcctgccgc ggtccctgaa ggggaaattg aacctgaccg tgaggtaccc cgagttctcg
     1441 aggaggtgcg ccatgtgcac gaggagggga tggaacatgt cgttgcccgc cccgtgagcc
     1501 aggatcacgg ccggcttcgt cccgctctgg tagtcgtcgg gcatgctgac aagcgtcgtg
     1561 atcttctcgc cgtactgacc gggtacgaac acatactcat ccgccgccat cctggcacct
     1621 cccttcgcgt tttcccgacc catcccggag ggtgatcagg ccgcgtgaat ctccttcaat
     1681 cacacatggg catgccttgt caaaagctca ggccgggcgt gcgcaggttc cgcacggtct
     1741 cgccgcggcc cgtacccgga ggac
//
LOCUS       DAOSCR010000582         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_45509_length_1749_cov_2.862456, whole genome shotgun sequence.
ACCESSION   DAOSCR010000582 DAOSCR010000000
VERSION     DAOSCR010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45509_length_1749_cov_2.862456"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PLT69_11785"
     CDS             complement(<1..1704)
                     /locus_tag="PLT69_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ15166.1"
                     /translation="MKKARWMMLLACVGLVLSSSACPLGNITVTILQTSDLHHHASGY
                     GPYADYTPLASDGDIVLGGYARLAALIKGIKERQNRRGMPTLLFDSGDFLMGTVYDLA
                     NENPPAFAFFQSMGYDAVTLGNHEFDWSPSGLALLLANAAANGFNVPVVATNMDVPEG
                     CALKGLVDSGVISRVKVLSHPCGVKIGIIGLMGRDADSKAPVARPVSFNHDYAFIQSQ
                     VDYLRKVEKVRVVIALSHGGVNEDGSGDDVDLANNVKGIDIIASGHYHTATSRPIVAG
                     PSRTIIFSPGEYGKYLSRLDVTYNVFTRKLVDYTFDLIPVDDGIVGDAGMDGLVKAYD
                     AGISAVLVSKGLPALKDPVSSTTFPLEMVPCSVTGLGALAADSLRAVANSLAPYNGGD
                     PFDVGIVASGVIRDEILGGVSGIITFADAYNVLPLGISPYDQSVPGYPLMSAYASGED
                     IYTICEVSLSLAPLMGSDYYLNFSGLRIDYDPSGAPTFSGVQSVEVYDPSDVFCLGPT
                     EPVDPDGTYRIVVDLYALQMLSVVNDYLIYYGLPPISPQNPSRIDRDPLTSDVQELKE
                     WM"
BASE COUNT          348 a          524 c          536 g          341 t
ORIGIN      
        1 catccattcc ttgagctcct gcacgtccga cgtgagtgga tctcggtcga tcctcgacgg
       61 attctgaggg cttatgggcg gaagaccata gtagatgaga tagtcgttca ccaccgagag
      121 catctggagt gcatagaggt ccacgacgat gcggtatgtc ccgtcaggat cgacgggctc
      181 tgtcggcccc agacagaaga cgtcagaagg atcgtagacc tccacgctct ggaccccact
      241 gaaggttggt gcacccgatg ggtcgtagtc gatcctgagt cccgagaagt tgaggtagta
      301 atcggatccc atgagggggg ccaggctgag ggagacctcg catatggtgt agatgtcttc
      361 tccggatgca tatgccgaca tgagcgggta ccctggcaca gactggtcgt acggggagat
      421 cccgagaggc aggacgttgt acgcatccgc gaacgtgatg atgccgctca cgcccccaag
      481 gatctcgtcc ctgatgacgc cgctcgccac gatgcccacg tcgaacgggt cacccccgtt
      541 gtatggggcc agggaattgg cgacagccct cagcgagtcc gctgccagtg caccgaggcc
      601 tgtaactgaa caaggtacca tctcgagggg gaaagtcgtg ctcgagaccg ggtccttgag
      661 ggcgggaagc cccttgctca cgagcaccgc gctgatgcct gcgtcgtagg ccttcacgag
      721 cccgtccata ccggcatccc cgacgatacc gtcatcgacc ggtatcaggt cgaacgtgta
      781 gtccacgagc ttcctggtga acacgttgta cgtcacgtcg agcctgctga ggtacttgcc
      841 gtattcgccg ggagagaaga tgatggtacg actcggtccg gcgactatcg gccgggatgt
      901 ggcggtgtgg tagtggcccg acgcgatgat gtctatcccc ttgacgttgt tggcgagatc
      961 cacgtcatcg ccgctgccgt cctcgttgac ccccccgtgg gacaacgcga tgaccactct
     1021 caccttctcc accttcctca ggtagtccac ctggctttgg atgaaggcat aatcgtgatt
     1081 gaagctcacg ggcctggcaa caggggcctt cgagtccgca tccctgccca tgagacctat
     1141 gatgcctatc ttcacgccgc aggggtgcga gaggaccttg accctcgaaa tgacaccgga
     1201 atccacgagc cccttgaggg cgcagccctc aggcacgtcc atgttggtcg ccaccaccgg
     1261 gacgttgaac ccgttggctg ccgcgttcgc cagaaggagc gcgagacccg agggcgacca
     1321 gtcgaactcg tgattgccca aggtcacggc atcgtacccc atggactgga agaaggcgaa
     1381 cgccgggggg ttctcgttgg cgaggtcata gacggtgccc atgagaaaat cgccggagtc
     1441 gaaaaggagc gtgggcatgc ccctcctgtt ctgcctttcc ttgatccctt tgatgagggc
     1501 ggcaagacgc gcataccccc caaggacgat gtcaccgtct gaagcgaggg gcgtatagtc
     1561 ggcgtacggg ccgtacccgc tcgcgtggtg gtgcaggtca gaggtctgca ggatcgtgac
     1621 ggtgatgttg cccagcgggc aggcgctgct cgacagcacg agcccgacac aagccagcag
     1681 catcatccaa cgtgcctttt tcataattca cctccctgtc ttcacgaaat caatacgcca
     1741 tggtcttct
//
LOCUS       DAOSCR010000583         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_45542_length_1748_cov_3.620201, whole genome shotgun sequence.
ACCESSION   DAOSCR010000583 DAOSCR010000000
VERSION     DAOSCR010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45542_length_1748_cov_3.620201"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1748)
                     /gene="dnaE"
                     /locus_tag="PLT69_11790"
     CDS             complement(<1..>1748)
                     /gene="dnaE"
                     /locus_tag="PLT69_11790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ15167.1"
                     /translation="KTACSFGQEAVAVTDHGVMHGVVEFYDKAVEAGIRPVIGCEVYV
                     APGDMTAREDIPGLPGSFHLILLAKDNKGYENLMKLSSLAHTKGFYDKPRVDRAALEA
                     LSEGVIGLSACLKGEIPYWLLRNEEAKAKEALDFYRRIYKGEFYLEIQHNGLPDQDAV
                     NPLIVELGRRTGTGVVATNDCHYLRPEDVEAHEVLLCLKTQKTLGDASRMTFSSDQLY
                     FKPTDVMERDFGFLPEAVSLSGEIAAKCDVAIPKNRYCFPVYPEESGRSLEEVIEEKA
                     RAGLVERMGPEVPETYVKRLEEELEIIKNMGFAGYFLIVADYIRYAKEHGIPVGPGRG
                     SAAGSLVAYCLRITDIDPIRWNLLFERFLNPERRSMPDIDVDFCQNRRDEVIAYVRDR
                     YGADYVCQITTFGNMKARAVIRDVGRVLGIPFGEVDRIAKLVPPRLDITLDEALQEEP
                     RLAELMEKDPRVNKLMAVARVLEGLSRHSSTHAGGVIISDRQPLTDHIPVYVDKHGML
                     ISQYDMKRIERVGLIKFDMLGLKTLTVIEKTLAILKGEGIELDLSSLPLDDEATYRLI
                     GEGDTTGVFQLESSGM"
BASE COUNT          273 a          575 c          542 g          358 t
ORIGIN      
        1 catgcccgag ctctcgagct ggaagacccc cgtggtgtcg ccctccccga tgagacggta
       61 cgtcgcctcg tcgtccaggg ggagggatga caggtccagc tcgatcccct cgcccttgag
      121 gatggccagc gtcttctcga tcacggtgag ggtcttcagc ccgagcatgt cgaacttgat
      181 gagcccgacc cgctcgatgc gcttcatgtc gtactggctg atgagcatcc cgtgcttgtc
      241 gacgtagact gggatgtgat cggtgagggg ctgcctgtcc gagatgatga cccctcccgc
      301 atgggtggag gaatgcctgc tcaggccctc caggacccgc gcgacagcca tgagcttgtt
      361 caccctcggg tccttctcca tgagttcggc gagcctcggt tcttcctgga gcgcctcgtc
      421 cagggtgatg tcgagacgcg gggggacgag cttggcgatg cggtcgacct cgccgaaggg
      481 aatcccgagg acccttccca cgtcgcggat gacggccctc gccttcatgt tgccgaacgt
      541 ggttatctgg cacacgtagt ccgcgccgta gcggtccctc acgtatgcaa tgacctcgtc
      601 ccgcctgttc tgacagaaat cgacgtcgat gtccggcatg ctcctgcgct cggggttgag
      661 aaacctctcg aagaggaggt tccaccggat ggggtcgatg tccgtgatcc tcaggcagta
      721 ggcgaccagg gacccggccg cgctccccct gccggggccg accggtatcc cgtgctcctt
      781 cgcgtagcgg atgtaatcgg ccacgatgag gaagtagcct gcgaaaccca tgttcttgat
      841 gatctccagc tcttcctcga gcctcttcac gtaggtctcg ggcacctcgg gccccatcct
      901 ctccacgagg cccgccctcg ccttctcctc gatgacttcc tcgaggctcc tgcccgattc
      961 ctccgggtag acggggaagc agtacctgtt cttggggatc gccacgtcgc acttggccgc
     1021 gatctcgccg ctcagcgaga cggcctcggg gagaaaaccg aaatcgcgtt ccatgacgtc
     1081 ggtgggcttg aagtagagct ggtcgctcga gaacgtcatc ctcgaggcgt cgccgagggt
     1141 cttctgcgtc ttgaggcaca agagcacctc gtgggcctcg acgtcctcgg gcctcaagta
     1201 gtggcagtcg ttcgtggcca cgaccccggt gccggttctc cttcccagtt ccacgatcaa
     1261 agggttgacc gcgtcctggt ccggcaggcc attgtgctgg atctcgaggt agaactcgcc
     1321 cttgtagatc ctccggtaaa agtcgagcgc ctccttcgcc tttgcctcct cgttcctgag
     1381 gagccagtag gggatctccc ccttgaggca ggcggacagg cctatgaccc cttcgctcag
     1441 cgcctcgagg gccgccctgt ccacgcgcgg cttgtcgtag aagcccttgg tgtgcgcgag
     1501 ggaggagagc ttcatgaggt tctcgtagcc cttgttgtct ttggccagga ggatgaggtg
     1561 gaagctgccg gggagcccgg ggatgtcctc ccttgccgtc atgtccccgg gcgcaacgta
     1621 gacctcgcag ccgatcacgg gccggatgcc agcctcgacg gccttgtcgt agaactcgac
     1681 cacgccgtgc atcaccccgt ggtccgtcac cgccaccgcc tcctggccga aggagcacgc
     1741 cgtcttga
//
LOCUS       DAOSCR010000584         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_45550_length_1748_cov_3.036621, whole genome shotgun sequence.
ACCESSION   DAOSCR010000584 DAOSCR010000000
VERSION     DAOSCR010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45550_length_1748_cov_3.036621"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1157)
                     /locus_tag="PLT69_11795"
     CDS             complement(360..1157)
                     /locus_tag="PLT69_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ15168.1"
                     /translation="MNRIILILSSISMLLLASGCARHTLGEENSALTAELAACRSGMA
                     KAGEENERLKAELSQARSKLEAAQTSLSESMDARQELLDKNIRCMEENQALLKQISRF
                     KTLTQERKDALARLTKAEQTLLSSLERERQANLLYVVKTESAVTVVIPQSSLFPSEFS
                     AWLTPKGIDLVRKVAKAIKDMRPSGLSVAGHTDGQPLPPSVSRIYPTLWDLAMARSVA
                     VLNVLEAAGIGKEKLSAVSYADTRPMADEKTDAGRAMNRRVEIVVLP"
     gene            1362..>1748
                     /locus_tag="PLT69_11800"
     CDS             1362..>1748
                     /locus_tag="PLT69_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ15169.1"
                     /translation="MIVARYMFGRLLAVFSGTAAALFGVMFAVQWMRLGRALGIGDVD
                     ILLLTLVPLSTFVIPMSLLFTVLLVIEKLSVDSEIVAMRASGVRDTLLFMPIVVFSLC
                     LALVHLAVSTTIGPLAMKAVHGDLMRS"
BASE COUNT          266 a          564 c          573 g          345 t
ORIGIN      
        1 ttggccttca cctggcgccc ccggcgatcg aggcgtaccg gcagggctca tgaaaggcgc
       61 ctgggacgcg ggctcgcatg cctcaaaagg ccgccccgag gatgacggtc ccggcggcgg
      121 tttcccaggt tgccgctgcc gggcttgaac ctttacgcgg acggccgggg catgtgccct
      181 cacgccgcgg gaacaccaat gttggaccgc cagcttgaac ctttctgttc ggccgggtcg
      241 aggggcgggt gagggccggg gcgctcacgc ctgcgggcaa gaccgcgaca ccgtgtgctc
      301 ccggggcgtc gccacgaacc tgtgcgtgcc ttccccgaga ggtgcgcccc actcttgggt
      361 cagggcagca ccacgatctc aacccgcctg ttcatggcgc gcccggcatc cgtcttctcg
      421 tcggccatgg gcctggtgtc ggcgtacgag acggcggaga gcttttcctt gccgatgcca
      481 gcagcttcga gtacgttcaa caccgccacg gacctcgcca tggcgagatc ccaaagcgtg
      541 ggatagatcc tcgagaccga cggcgggaga ggctgtccgt cggtatggcc ggccaccgac
      601 agaccggaag ggcgcatgtc cttgatcgcc ttcgcgacct tcctcaccag gtcgataccc
      661 ttcggcgtga gccatgcgct gaactccgag gggaacagcg atgactgcgg tatcaccacg
      721 gtcaccgcgc tctcggtctt gaccacgtag aggaggtttg cctggcgttc ccgctcgagg
      781 gacgacagga gcgtctgctc ggccttggtg aggcgggcca gcgcgtcctt gcgctcctgg
      841 gtgagcgtct tgaacctgga gatctgcttc aagagggcct ggttttcctc catgcagcgg
      901 atgttcttgt cgagaagctc ctgccttgcg tccatggact cggacaagga ggtctgggcg
      961 gcctccagct tggaacgcgc ctgcgacagc tccgccttca ggcgctcgtt ctcttcgccc
     1021 gccttcgcca tgccggaccg gcacgcagcg agctcggccg taagggccga gttttcttcc
     1081 ccgagggtgt gccgcgcgca gcctgatgcg aggagaagca tggatatgct cgataaaatg
     1141 agaattattc tgttcatttg cagggacacc ataaaccctc gggcgcgttc atgtcaacga
     1201 gcgcgtgggc gcatcgtggg ggggccttac gatgcacacg gccggcccgt agatgcaaaa
     1261 aagcggtgtg cgcaccctcg cgaggccctc cccacggtgt ccgtctccgg gaacgcgaag
     1321 tggactttgt cctggattcg caggtataat cgaatcccgc catgatcgtg gccaggtaca
     1381 tgttcgggcg cctgctggcg gtcttctccg gcaccgcggc ggcgctcttc ggggtcatgt
     1441 tcgcggtcca gtggatgcgc ctgggccgcg cgctgggcat tggggacgtg gacatcctgc
     1501 tgctcaccct cgtgccgttg tcgaccttcg tcatacccat gtctctcctg tttaccgtcc
     1561 tcctggtgat cgagaagctc tcggtggact cggagatcgt cgcgatgagg gcgagcgggg
     1621 tgagggacac cctcctcttc atgcccatcg tggtcttcag cctctgcttg gcgctggtcc
     1681 acctcgcggt gtccacgacg atcggtccgc tcgccatgaa ggccgtccac ggcgacctga
     1741 tgaggtct
//
LOCUS       DAOSCR010000585         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_45601_length_1747_cov_2.401300, whole genome shotgun sequence.
ACCESSION   DAOSCR010000585 DAOSCR010000000
VERSION     DAOSCR010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45601_length_1747_cov_2.401300"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLT69_11805"
     CDS             <1..561
                     /locus_tag="PLT69_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15170.1"
                     /translation="GSIGIPFPDNDVRLVDVEEGIHEVKQGEPGEIVMKGPLIMKGYW
                     NNPEETKAQIRDGWLHTGDVAVTDTDGYIYIVDRKKDMIIAGGFNIYPREIDEVLYQH
                     PKVAEAVSIGVPDEYRGETVKAFIVLKPGERATEREIIDFCKERLTAYKVPKMVEFRD
                     TLPKSAVGKILRKILREEEAQRRGKS"
     gene            complement(578..>1747)
                     /locus_tag="PLT69_11810"
     CDS             complement(578..>1747)
                     /locus_tag="PLT69_11810"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ15171.1"
                     /translation="RLAPQRSPRHVVWKRVMDMNDRCLRSIILGLEDRGLNGVMREDG
                     FEITAASEIMAVLCLSKDLREVKERIENIIVGYDYLKKPVFARDIGGAGAMAALLKHA
                     INPNLVQSVEGVPVFVHGGPFANIAHGCNSLVATNLALRLADYVVTEAGFGVDLGAEK
                     FFHIKCRTGGLTPSAAVMVVTVRAYRLHGMENIRKHLDTLDRFGVPSVISVNRFASDK
                     EGDLEELAGRCRDLGAEVVVTDFRESGGEGGLELAQKVVDLCSRQAAFRMLYDLSMPL
                     ADKVRTVAREVYGASGVEFTPQAENDLVAIENMGYGNLPVCIAKTQSSLTDNPKIPGF
                     PKEPFTIKVSSARVLSGAGFVVVYTGRILAMPGLPRVPAALAIDVDENGTISGLF"
BASE COUNT          338 a          555 c          561 g          293 t
ORIGIN      
        1 gggtccatcg gcatcccgtt cccggacaac gacgtgaggc tcgttgacgt ggaggagggc
       61 atccacgagg tgaaacaggg cgagcccggc gagatcgtca tgaaaggccc cctcatcatg
      121 aagggatact ggaacaaccc cgaggagacc aaggcccaga tacgggacgg gtggctccat
      181 accggggacg tggcggtcac ggacaccgac gggtacatct acatcgtcga ccgcaagaag
      241 gacatgatca tcgcaggcgg tttcaacatc taccccaggg agatcgacga ggtgctctac
      301 cagcacccga aggtggccga ggcggtgagc ataggcgttc ccgacgagta ccggggggag
      361 acggtgaagg ccttcatcgt gctgaaaccg ggggagcgcg ccaccgagcg ggaaatcatc
      421 gatttctgca aggagaggct cacggcatac aaggtcccca agatggtcga gttcagggac
      481 accctgccga agagcgcggt cggcaagatc ctgaggaaga tcctgaggga ggaggaggcc
      541 caaaggaggg ggaagtcgtg agggcgtgat gcggccctca gaagagcccg ctgatcgtgc
      601 cgttctcgtc cacgtcgatg gctagggctg cagggaccct gggcaggccg ggcatggcca
      661 ggatccggcc cgtgtacacc accacgaacc ccgccccgga caagacgcgt gcggaagaga
      721 cctttatggt gaaaggttcc ttcggaaatc ccgggatctt gggattgtcc gtcagggaag
      781 actgtgtctt ggcgatgcag acgggaaggt tcccataccc catgttctcg atggccacga
      841 gatcgttttc ggcctggggc gtgaactcca cgccggaagc cccgtacacc tcccgcgcca
      901 cggtcctcac cttgtcggca aggggcatgg agagatcgta gagcatccgg aacgccgcct
      961 gcctcgagca cagatcgacc accttttggg cgagctcgag ccccccttcg ccgcctgatt
     1021 ccctgaagtc cgtgacgaca acctccgccc ccaggtcgcg gcagcggccg gccagttcct
     1081 cgagatcgcc ctccttgtcg gatgcgaacc ggttgaccga gatcaccgac ggtacgccga
     1141 accggtcgag ggtgtccagg tgcttgcgga tgttctccat cccgtgaagt cggtacgcac
     1201 gcaccgtgac caccatgacg gcggccgacg gcgtgagccc ccctgtcctg cacttgatgt
     1261 gaaagaactt ctcggcgccc aggtccaccc cgaagcccgc ctcggtgacc acgtagtcgg
     1321 ccagcctgag cgccaagttc gtggccacga gtgagttgca cccgtgcgcg atgttcgcga
     1381 agggcccacc gtggacgaac accggcaccc cttccaccga ctgcacgagg ttcgggttga
     1441 tcgcgtgctt gaggagggcg gccatggccc ccgccccccc tatgtcccgg gcgaacacgg
     1501 gttttttgag gtagtcgtac cccactatga tgttctcgat gcgttccttc acttccctga
     1561 gatccttgga caggcacaac acggccatga tctcgctggc ggccgtgatc tcgaacccgt
     1621 cctcgcgcat gacgccgttg agccccctgt cctccaggcc gaggatgatg ctcctcaggc
     1681 acctgtcgtt catgtccatg acgcgcttcc agacgacgtg ccgcgggctg cgctgcgggg
     1741 cgaggcg
//
LOCUS       DAOSCR010000586         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_45876_length_1739_cov_2.663895, whole genome shotgun sequence.
ACCESSION   DAOSCR010000586 DAOSCR010000000
VERSION     DAOSCR010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45876_length_1739_cov_2.663895"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          281 a          478 c          641 g          339 t
ORIGIN      
        1 cccagaagtt ggggtgtgca cccagaagat ggggtgtgca aaccgtaaag acggtgtttc
       61 ccctgtcccg gagggctctg catctctgca tcgtgggggg gcgtaaaggg ctgccgatgg
      121 aggggtcatg tgaggaaggc cgtccagccc gccttacctg agggcgcctc gtgagagggg
      181 ttgtacacta cgcgtgacgc caggcgcgaa gagcttgcct gtgcgagccc gccgcggcca
      241 cgtagatcgc tcggctccag ggccacgcgt gccggccggc gcgcagacct tgcctgtgcg
      301 tgtcgagagg gctaaagaag tgtggccgcg gtcggggaac gcgcggctgg atatgccgcg
      361 ctccattgcc cgtcgtgggt gacgtgccgt tggtgttcca tgtgatcgca ggtgccgctg
      421 agaggctcgg ctgacctccg gtaaggcggg tggatccgct ccttgcgtgt ggtgcgatcg
      481 cagaggcccg gctcaatgaa tgaggctcgt cgccgtgggg aagatcatcg gctttgagcc
      541 ggtgcgaccc ggtaccggtc gcctctacga tgcctaaggc gtttgcccca aggacgctca
      601 aggtgttgtc gtcctttggg aagctcctgg gtgtggtccc aacggatcga gatcctgaga
      661 gggtccgtcg agctccacga ggacttgccc ccacctgtgc ctggtctggg gggaggcggg
      721 ggtcgggagg gtgaaggccg gggtgtgcgg ggaagggggg cgatcgcggc agaccgatcg
      781 ggccatgtgc acgagccagg gtccccagtg ggctgtcgcc gcctgaagtg caggaacgag
      841 ggaggcttgt cctgccatgg gcgcgatcga gtacccggct gcctcgtacg gccggggacg
      901 cccaagcgct ggataccgag ggtcccgggt atcgccagct ggagaccctc tcgaacccga
      961 gggcccaggc ggccggagcc ggagcggtcc atctcgtcga aacccatctc cctagacact
     1021 ctccccttct atccagaggg atccgaacat gcgtgatcgc gaggcctggc gccctcggtg
     1081 atggaagtcg gagaagagag aggaaaaggt atgcgaattg catgttagtc ggttgacacg
     1141 atggagcgtt ttcacaataa tgaggattca ttactatagg gagttgatcg atcgccctga
     1201 aagtactatg atgcgtacca tggcgagacc agtgagcgtg aaccttgcct gcccggtgat
     1261 catgcacggt gggccggacc gcgagggaag acagggtgat ggcttctgtg ccgctttctt
     1321 ccagggtcat gccttcggcc tgggccgagg cccttctccg gggtggactc tctccgccgg
     1381 ttctgttgcg ctcgatggtg catccatgct cgatggtgca tccatggacc aaggcctctt
     1441 tggaccctgt ggcaggggct tggtgaaggg tgcgaggcgc gcccgcgttt ttgtaccgtc
     1501 ccttccttca aggcctatgg gtcttcgggg aaggcgggcg cggcgcatgg gccgagccct
     1561 gttcacggtg ggttctcaag gatacgaacg cgcgcgacag gctgctggcg tgcgcgaagg
     1621 agggtgagct gtgaggatgc gcatggggga tctcggaaag gttgtcttct gtctggtctt
     1681 ggcgttgacg atcgtctcct gctcgggcag cgacggttcg ggagggaagg cctcgggcg
//
LOCUS       DAOSCR010000587         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_46548_length_1722_cov_2.153569, whole genome shotgun sequence.
ACCESSION   DAOSCR010000587 DAOSCR010000000
VERSION     DAOSCR010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46548_length_1722_cov_2.153569"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..>1722)
                     /gene="acs"
                     /locus_tag="PLT69_11815"
     CDS             complement(217..>1722)
                     /gene="acs"
                     /locus_tag="PLT69_11815"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOJ15172.1"
                     /translation="IAMLACTRIGAIHTIVFGGFSSDALRDRILDSEANLLITCDGTY
                     RGNKAVPQKDNADKAAEQCPTIKKMIVVKRTGTPVNWVEGRDVWWEDEMKDAPYYCEP
                     EPMNAEDPLFILYTSGSTGKPKGVMHTTGGYLVYVSYTHQIIFDYHPEDIYWCTADIG
                     WVTGHSYIVYGPLYNGATSIMFEGVPSYPGFDRFWDVVEKFKVDIFYTAPTAIRAIAK
                     EGDHWVDKHDLSSIRILGSVGEPLNPEAWRWYYNKIGRGECTIVDTWWQTETGGILIT
                     PLPGAIDIKPAKATVPFFGVEPCIVDDDGKEIEGPGRGNLCIKRPWPGQMRGVWGDPK
                     RFEETYFVQFPGKYFTGDGAERDELGYYKITGRVDDVINVSGHRMGTAEIESALVSHP
                     DVAEAAVVGFPHDIKGQGIFAYVTLNTGVAKSEDLKKALVAHVRKEIGPIATPDQILW
                     ADALPKTRSGKIMRRILKKIAAADFSDFGDTSTLADPTVVDTLVAGRKELK"
BASE COUNT          262 a          525 c          574 g          361 t
ORIGIN      
        1 gaatccctcc atggcgaaca cttgcatcgc cgcattcaat atcatgcgat atttctcttc
       61 acccctcatg gtgtcccctt tatgttgtca tggcaggaga ggctgccatg gaacgaaacg
      121 ctcagtccgt gcactcaggt atgtatgatc gcgttgtgta tgatcgttcc gggggcgggg
      181 ctcggcccgc ccccgggtta tctcgtgatg cctaggctac ttgagctcct tcctccccgc
      241 cacgagggtg tccacgacgg tcggatccgc cagcgtcgag gtgtcgccga agtcggagaa
      301 gtcggctgcc gcgatcttct tgagaatccg gcgcatgatc ttcccggagc gggtcttggg
      361 cagggcgtcc gcccagagga tctggtccgg ggtcgctatc gggccgatct ccttgcgcac
      421 gtgcgcgacg agcgccttct tgaggtcttc gctcttggcc acgccggtgt tgagcgtcac
      481 gtaggcgaag atgccctggc ccttgatgtc gtgcgggaat cccacgaccg ccgcctcggc
      541 gacgtccggg tgggaaacga gcgcgctctc gatctcggcc gtgcccatgc ggtggccgga
      601 gacgttgatg acgtcgtcca cgcggccggt gatcttgtag tagccgagtt cgtcgcgctc
      661 ggcgccgtca ccggtgaagt acttgcccgg gaactggacg aagtaggtct cctcgaagcg
      721 cttggggtcg ccccacacgc ccctcatctg gccgggccag ggcctcttga tgcagaggtt
      781 gcccctgccg ggaccctcga tctccttgcc gtcgtcgtcc acgatgcagg gttcgacgcc
      841 gaagaagggg acggtggcct tggcgggctt gatgtcgatg gcgcccggca acggggtgat
      901 gaggataccg cccgtctcgg tctgccacca ggtgtcgacg atggtgcact cgccgcggcc
      961 gatcttgttg tagtaccatc tccacgcctc ggggttcagc ggttcgccca cggaacccag
     1021 gatgcggatg gaggagaggt cgtgcttgtc gacccagtgg tcaccctctt tcgcgatggc
     1081 gcggatcgcc gtgggcgccg tgtagaagat gtcgaccttg aacttctcga ccacgtccca
     1141 gaacctgtcg aagccgggat agctgggaac gccctcgaac atgatgctcg tcgcgccgtt
     1201 gtacagcggc ccgtacacga tgtagctgtg gcccgtcacc cagccgatgt cggccgtgca
     1261 ccagtagatg tcctcgggat ggtagtcgaa gatgatctgg tgcgtgtagg acacgtagac
     1321 caggtagccg cctgtggtgt gcatgacgcc cttgggcttg ccggtggacc cggaggtgta
     1381 gaggatgaag agcgggtcct ccgcgttcat gggctcgggc tcgcagtagt acggggcatc
     1441 cttcatctcg tcttcccacc acacgtcccg gccttccacc cagttcacgg gcgtgccggt
     1501 cctcttgacc acgatcatct tcttgatggt ggggcactgc tcggccgcct tgtcggcgtt
     1561 gtccttctgg ggcacggcct tgttgccgcg gtaggtgccg tcgcaggtga tgaggaggtt
     1621 ggcctcgctg tcaaggatgc ggtcgcgaag ggcgtccgag gagaacccgc cgaacacgat
     1681 ggtgtggata gcgccgatgc gggtgcaggc cagcatggcg at
//
LOCUS       DAOSCR010000588         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_46716_length_1717_cov_2.929603, whole genome shotgun sequence.
ACCESSION   DAOSCR010000588 DAOSCR010000000
VERSION     DAOSCR010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46716_length_1717_cov_2.929603"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLT69_11820"
     CDS             complement(<1..573)
                     /locus_tag="PLT69_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOJ15173.1"
                     /translation="MGRKEGEAMETNPGVVSEDADVIRKVVEELCRPESYSEVYHRPT
                     LEGVMPSLEVLSEVVERLRMVLFPAYFSDPEMTPATMPYIVGSHLERVERLLAEQIKR
                     GFGFFCEQTDRASCIECDERSRVLARRFIVKVPEIRRLLATDVMAAFEGDPAAKSTSE
                     TIFCYPSIKAMTNYRIAHELHLLGVEIIPRI"
     gene            complement(582..1205)
                     /locus_tag="PLT69_11825"
     CDS             complement(582..1205)
                     /locus_tag="PLT69_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOJ15174.1"
                     /translation="MDKVGWVYSDKVLDHFMHPRNILEDEQAFQADGVGVTGNVKCGD
                     QMIVAIKVDAERGVITDCKWKTFGCASAIASTSIMSEMVKGMRLEDAFRLSPRDINKE
                     LGGLPEHKIHCSVLGDKALRAAINDYYEKTGQAEKIQREEARVVCSCMNVTDHEIEEA
                     VLEGARSLYEVQEKTKVATVCGECTEEVERLIEFYTNKHFCATEGRT"
     gene            complement(1205..>1717)
                     /locus_tag="PLT69_11830"
     CDS             complement(1205..>1717)
                     /locus_tag="PLT69_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ15175.1"
                     /translation="PFCPFIRGGHQERGRRAGTENTIGIAALGKAVELRDLEMDAEGE
                     RLRAMKERLKEGIAAAIPDVHFVGHPTDCLAGTLNVSFEGAEGEAILLYLDLAGIAVS
                     TGSACASGSLDPSHVILATGLSPECAHGSIRISMGRENTMEDVDYFLDVLPGVISRIR
                     KMSSAYAGRK"
BASE COUNT          258 a          614 c          503 g          342 t
ORIGIN      
        1 gatgcggggg atgatctcca cgcccaaaag atgcagctcg tgggcgatgc ggtagttggt
       61 catggccttg atgctcggat agcagaagat cgtctcgctc gtgctcttgg ccgcagggtc
      121 cccctcgaag gcggccatga cgtcggtggc gagcagcctc ctgatctccg gcaccttcac
      181 gatgaacctc cgcgcgagaa ccctcgagcg ctcgtcgcac tcgatgcagg aagccctgtc
      241 cgtctgctcg cagaagaagc cgaaaccccg cttgatctgc tccgcgagca ggcgttccac
      301 cctctccagg tggctgccca cgatgtaggg catggtcgcc ggggtcatct ccgggtcgct
      361 gaagtaggcg gggaacagca ccatgcggag gcgttccacc acctcggaga ggacctccag
      421 ggagggcatc acgccctcga gcgtcggcct gtggtagacc tcgctgtagg actccggcct
      481 gcagagctcc tcgacgacct tccgtatcac gtccgcgtct tcgctcacga ccccggggtt
      541 cgtctccatg gcctcgcctt ccttccgtcc catgggtgag ttcacgtccg cccctcggtg
      601 gcgcagaagt gcttgttggt gtagaactcg atgagcctct ccacctcctc ggtgcactcc
      661 ccgcacacgg tcgccacctt cgtcttctcc tggacctcgt agagcgacct cgcgccttcc
      721 agcaccgcct cctcgatctc gtggtcggtc acgttcatgc acgagcagac cacgcgcgcc
      781 tcctccctct ggatcttctc ggcctggccg gtcttctcgt agtagtcgtt gatggccgcg
      841 cgcagcgcct tgtctcccag gacgctgcag tggatcttgt gctctggcag accgcccagc
      901 tccttgttga tgtccctggg cgacagcctg aacgcatcct cgagcctcat acccttgacc
      961 atctccgaca tgatcgaggt gctcgcgatg gcgctcgcgc acccgaaggt cttccacttg
     1021 cagtccgtga tgacgccacg ctcggcgtcc accttgatcg ccacgatcat ctggtcgccg
     1081 cacttgacgt ttccggtcac ccccacgccg tcggcctgaa acgcctgttc gtcctcgagg
     1141 atgttcctcg ggtgcatgaa gtgatcgagg accttgtcgg agtaaaccca ccctacctta
     1201 tccatcactt cctccccgcg taggcgctcg acatcttcct gatgcgggaa atgacacccg
     1261 gcaggacgtc gaggaagtag tcgacgtcct ccatggtgtt ctccctcccc atgctgatcc
     1321 gtatggaccc gtgcgcgcac tccgggctca gccccgtggc gaggatcacg tgcgaggggt
     1381 cgagcgaccc ggaggcgcag gccgaccccg tcgacaccgc gatgcccgcg aggtcgaggt
     1441 acaggaggat ggcctccccc tccgcaccct cgaaggagac gttcagggtc ccggccaggc
     1501 agtcggtggg gtgtcccacg aagtgcacgt ctgggatcgc ggcggcgatg ccttccttca
     1561 gccgttcctt catcgcgcgc aggcgttcgc cttccgcgtc catctccagg tccctgagct
     1621 cgaccgcctt gccgagcgcc gcgatcccga tggtgttctc ggtcccggcc ctccgccccc
     1681 tctcctggtg ccccccccgg atgaaggggc agaacgg
//
LOCUS       DAOSCR010000589         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_46721_length_1717_cov_2.655235, whole genome shotgun sequence.
ACCESSION   DAOSCR010000589 DAOSCR010000000
VERSION     DAOSCR010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46721_length_1717_cov_2.655235"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLT69_11835"
     CDS             complement(<1..515)
                     /locus_tag="PLT69_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOJ15176.1"
                     /translation="MRKGIVAWQAAYAFVLVTAVGGCAYISMDLGRLVEMQPFEERVI
                     REGGPDKVLVLEILGPITTTAFRDGFMPRQGTLERLDSILELAGKDRRIKGVILKIDS
                     PGGGITASNLLYREILEYKEKRRTSVVACITGQGTSGAYMAALAADSIVALPTSVVGN
                     VGVLLPSISLEG"
     gene            complement(508..>1717)
                     /locus_tag="PLT69_11840"
     CDS             complement(508..>1717)
                     /locus_tag="PLT69_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOJ15177.1"
                     /translation="DQCAFACTNDASAIDELADTVARAVGSGEFSRDFSLLRRTVGSV
                     VRMQVDPRGVDPVLKLSRVREADSAARGMDPAVVQVRVMYADGMKRVIVANSLGFVAQ
                     DRRDSIIFVVQGIASDGSVMETGYEPVGGCRGFELLDETPPEEIARRAVGRALTMLRA
                     RRSPAGVMPVVLSSEAGGTMIHEAIGHGLEADLAGEGLSVYSGKLGQEVASKAITVID
                     DGTIEGVRGSMGCDDEGNPTCRTVLVEGGVLKGYMTDLVTSRKYGLPLTGNARRESYR
                     HRPIPRMTNTMIAPGTLDPQEIVRLVHRGLFVKKMGGGQVNTVTGDFVFNVAEGYLIE
                     NGQIAEPVRGATLIGNGPRILSSIDKVACDLGFGIGTCGKDGQGVPVADAQPTLLIPE
                     ITVGGEIRDA"
BASE COUNT          258 a          607 c          554 g          298 t
ORIGIN      
        1 ccctcgaggc tgatggaggg aaggagcacg cccacgttgc cgactaccga ggtcggcagg
       61 gccacgatgg agtccgcggc cagggcggcc atgtacgcac ccgaggtccc ctggccggtg
      121 atgcacgcga ccacgctcgt gcggcgcttc tccttgtact cgaggatctc cctgtagagg
      181 aggttggacg ccgttatccc ccctcccggc gagtctatct tgaggatgac gcccttgatg
      241 cgcctgtctt tgccggccag ctccaggatc gagtccaggc gctcgagcgt gccctgcctg
      301 ggcatgaacc cgtccctgaa cgcggtggtc gtgatggggc ccaggatctc gagcacgagc
      361 accttgtcgg gcccgccctc cctgatcacc cgttcctcga agggctgcat ctccacgagc
      421 ctgcccaggt ccatggatat gtaggcgcag ccgcccacgg ccgtgacgag cacgaaggcg
      481 tacgcggcct gccatgcaac gataccctta cgcatcgcgt atctcccccc caacggtgat
      541 ctccgggatg agcagggtgg gctgggcgtc ggccacgggc acgccctgcc cgtccttgcc
      601 gcaggttcct atgccgaaac cgaggtcgca agcgaccttg tcgatcgacg aaaggatcct
      661 cgggccgttg ccgatcaggg tggccccgcg cacgggctcg gcgatctgtc cgttctcgat
      721 gaggtaccct tccgccacgt tgaacacgaa gtcgccggtg accgtgttca cctgcccgcc
      781 gcccatcttc ttcacgaaca gcccacggtg tacgagcctg acgatctctt gggggtcgag
      841 ggtcccggga gcgatcatgg tgttggtcat gcgggggatc ggcctgtgcc tgtacgactc
      901 gcgcctggcg tttcccgtca ggggcaggcc gtacttgcgc gaggtgacga ggtccgtcat
      961 ataccctttc agcaccccac cctcgacgag gacggtccgg catgtcgggt tcccctcgtc
     1021 gtcgcatccc atgctgccgc gcaccccctc gatggtgccg tcgtcgatga cggttatggc
     1081 cttggaggcc acctcctggc ccagtttccc cgagtagacc gacagcccct cgcctgcgag
     1141 gtcggcctcg aggccgtggc cgatggcctc gtggatcatc gtgcccccgg cctcgctcga
     1201 gagcaccacc ggcatgacgc ccgccggcga cctccttgcc ctgagcatgg tgagggccct
     1261 gccgacggcc ctgcgcgcta tctcctccgg gggggtctcg tccaggagct cgaacccccg
     1321 gcagccgccc accggctcgt aaccggtctc catgacggaa ccgtcgctcg ctatgccctg
     1381 caccacgaag atgatcgagt cgcgccggtc ctgggccacg aagcccaggg agttcgcgac
     1441 gatgacccgc ttcatgccgt cggcgtacat gacgcggacc tggaccacgg ctgggtccat
     1501 gcctcgtgcc gcactgtcgg cctcgcggac ccgggagagc ttcaggacgg gatcgacgcc
     1561 cctggggtcg acctgcatgc gcacgactga accgacggtg cgcctgagga gggagaagtc
     1621 cctcgagaac tcgccggagc cgaccgcgcg ggccacggtg tcggcgagct cgtcgatcgc
     1681 cgaggcgtcg ttcgtgcatg cgaacgcgca ctgatcg
//
LOCUS       DAOSCR010000590         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_46793_length_1715_cov_3.307831, whole genome shotgun sequence.
ACCESSION   DAOSCR010000590 DAOSCR010000000
VERSION     DAOSCR010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46793_length_1715_cov_3.307831"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..787)
                     /locus_tag="PLT69_11845"
     CDS             complement(125..787)
                     /locus_tag="PLT69_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOJ15178.1"
                     /translation="MTGLHGGGLVFVAGRVLEEGMDMPVFFDSPIYTYLVLPLLIFSA
                     RIMDVSLDTIRIIFINRNLRYLATAVGFFEVLIWLMVIRQIFQQLDNPVCFVAYAAGF
                     AAGNFVGIIIENRLSIGRVIIRIITRQEADDLVSYLRSEGYGITVIDAEGATGPVEVI
                     FTIVERSDIGRVVEIIKRFNPQSFYSVEDVRFVSEAVTPYRRLPTRRWGRFTSRMRRD
                     DR"
     gene            complement(926..1600)
                     /locus_tag="PLT69_11850"
     CDS             complement(926..1600)
                     /locus_tag="PLT69_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOJ15179.1"
                     /translation="MEKTPATFFRKLHDPVSGLTHCIGAVLAVAGLVALIVKATNPVR
                     PWHLSTYTVFGAGMILLYTASTLYHWLPVSSRTKAHLRRLDHMMIFVLIAATYTPFCL
                     IPLRGAWGWGIFGTVWTVAALGIAFKMVWINAPRWLSAAMYVCMGWIALAGIVPLVRA
                     LQGGALFWLFAGGVLYTAGALVYATKKPDPIPRVFGFHEIFHIFVMLGTMAHFWTVYK
                     YVSMFS"
BASE COUNT          317 a          564 c          545 g          289 t
ORIGIN      
        1 aaccggccgc ccgtgccttt tccgcctccc gggacggtga gcgggggtac gcgcttgaag
       61 cgtgggtctc tgcgggccgg gggcccgttc acgtccccgt tccaaacggg tgccttcctt
      121 aagctcagcg atcgtccctc cgcatgcgcg acgtgaacct gccccacctc ctcgtcggca
      181 aacggcggta gggggtcacg gcctcgctga cgaagcggac gtcctccacc gagtagaacg
      241 actgggggtt gaaccgcttg atgatctcca ccacgcgccc tatgtcgctg cgctcgacga
      301 tggtgaagat gacctcgacc ggtcccgtgg ccccctcggc gtcgatcacg gtgatcccgt
      361 acccttccga gcggaggtac gacaccaggt cgtccgcctc ctggcgcgtg atgatccgga
      421 tgatgaccct cccgatggag agcctgttct cgatgatgat gcccacgaag ttccccgctg
      481 cgaaccctgc cgcgtacgcc acgaagcaca cggggttgtc cagctgctgg aagatctggc
      541 ggatcaccat gagccagatg aggacctcga agaaccctac ggccgtagcc aggtatctga
      601 gattgcggtt gatgaagatg atcctgatcg tgtcgaggct cacgtccatg atgcgggctg
      661 agaagatgag caggggcagc accaggtagg tgtagatcgg cgagtcgaaa aagacgggca
      721 tgtccatgcc ctcttctaac acccggccgg ccacgaacac aaggccgccc ccgtggagtc
      781 cggtcatgcg cctttccacc cagtgcaggg tatcaccccg ggcggttgat gatcgaagga
      841 cccggacagg ccagccctct cacgccccag tggccttttc cacgctcacc ccgggcgaga
      901 tctcgggcgg tgtgggccgc cgtgctcagg aaaacatgct cacgtacttg tacacggtcc
      961 agaagtgggc catggtgccc agcatgacga agatgtggaa gatctcgtgg aacccgaaca
     1021 cgcggggtat gggatcgggc ttcttggtcg cgtacacgag ggcgccggcg gtgtagagga
     1081 cacccccggc gaagagccag aacagcgctc ccccctggag ggccctgacg agcggcacga
     1141 tgccggcgag cgcgatccac cccatgcaca cgtacatggc cgcggaaagc cacctcggcg
     1201 cattgatcca caccatcttg aaggcgatgc cgagcgccgc gacggtccac accgtgccga
     1261 agatgcccca cccccacgca ccgcgcagcg ggatgaggca gaacggggtg taggtcgccg
     1321 cgatgagcac gaagatcatc atgtggtcga gccgcctcaa gtgcgccttc gtcctgcttg
     1381 agaccggcag ccagtggtag agcgtgctcg ccgtgtagag gaggatcatg cccgccccga
     1441 acaccgtgta cgtgctcagg tgccagggcc tcacagggtt cgtcgccttc acgatgagag
     1501 ctaccaggcc ggccacggcg agaacggcgc cgatgcagtg ggtgagaccg ctcaccgggt
     1561 cgtgcaactt ccggaaaaac gttgcaggtg tcttttccat acaggtgtct tcttccctcc
     1621 cggccgcagt ggccgccatc gaccggtgga gcatgggcac gacgccccgg acccccgttc
     1681 gagtgtcgtg ccggccatgt tctcctatcc cggcg
//
LOCUS       DAOSCR010000591         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_46801_length_1715_cov_2.719880, whole genome shotgun sequence.
ACCESSION   DAOSCR010000591 DAOSCR010000000
VERSION     DAOSCR010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46801_length_1715_cov_2.719880"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PLT69_11855"
     CDS             <1..1639
                     /locus_tag="PLT69_11855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ15180.1"
                     /translation="DFGLWGDRAVTGMHVQSIVLYAFMVLACYGFLRRLLQIFHDDEE
                     EGYLWAAMASVLYAVHPVHVESVAWLFARKEPLLGIFTFLSLWAFIEARVTSWWYYLP
                     CLATLVLAVLAKPTALVAPAVMFVLDLAIQKRRPDPAFWKKRLFVYLPMLAFTVPMAY
                     RLVDMMQEAGGVKPYHGGGFWTNLLAVSQIFVSYIKLAGFTVNYSADYPIPLYTDPFS
                     WRPWVYVAVNVILLASAVYAFMRKRLVYAFFVAWHYLFVLPVSHVFPIAQIMADRYAL
                     LPSLSWCVLVGYLLAKLWTVRKTVWSLSPGFFTALSVGLFCFVTLFYAYMTFRQNDIW
                     QNSQSLWEDTLAKYPDSSPANVNLAAIYIKQGRPLEAQELAIRAIRVLPYDYLAISNL
                     ALAQVMMGQYDNAISNYKAALRLKPSLIEARMGLAHAYWEKGDYENAYSVYMQLYQEG
                     VVAGSRHAPTMYFRLGYMTWKYGDPRDARAFLAAAEPGLRGDLFKLSELAGVYTSMND
                     FRNAYRVYRTVYDRLEEGEPKAKLARLLSALEKKMRQ"
BASE COUNT          304 a          547 c          529 g          335 t
ORIGIN      
        1 cgacttcggc ctctggggcg acagggcggt gaccgggatg cacgtgcaga gcatcgtgct
       61 gtacgcgttc atggtgctcg cctgttacgg gttcttgaga aggctccttc agatcttcca
      121 cgacgacgag gaggagggtt acctttgggc ggccatggcg agcgttctct atgccgtgca
      181 cccggtacac gtggagagcg tcgcgtggct cttcgcgcgc aaggagccgc tgctcgggat
      241 cttcaccttc ctgtcgctgt gggcgttcat cgaggcccgc gtgacatcct ggtggtatta
      301 cctgccctgc cttgcgaccc tcgttctcgc ggtactcgcc aagccgaccg cgctcgtggc
      361 gccggcggtc atgttcgtgc tggacctcgc gatccaaaaa cgtcggcccg accccgcatt
      421 ctggaagaag aggctcttcg tctacctccc catgctcgcc ttcacggtcc ccatggccta
      481 caggctcgtg gacatgatgc aggaggcggg cggggtgaag ccctaccacg gcgggggctt
      541 ctggacgaat ctcctggccg tgtcccagat cttcgtgagc tacatcaagc ttgcggggtt
      601 cacggtgaat tactcggcgg attacccgat acccctctac accgatccct tttcgtggcg
      661 tccgtgggtg tacgtggcgg tcaacgtcat ccttctggcc agcgcggtct acgccttcat
      721 gagaaaaagg ctcgtctacg ccttcttcgt ggcatggcac tacctgttcg tcctgcccgt
      781 ctcccacgtg ttccccatcg cgcagatcat ggcggacagg tatgcgctcc tgccctcgtt
      841 gagctggtgc gtccttgtcg gttacctcct ggcgaagctg tggacggtga ggaagaccgt
      901 gtggtcgctg tcgccgggtt tcttcaccgc gctctcggtg gggctgttct gtttcgtcac
      961 cctgttctac gcgtacatga ccttccgcca gaacgacatc tggcagaaca gccagagcct
     1021 ctgggaggac acccttgcca agtacccgga ctcgagcccg gcaaacgtca acctcgccgc
     1081 catctacata aagcagggca ggcccctgga ggcccaggaa ctggccatcc gagccatcag
     1141 ggtccttccc tacgactacc tggccataag caacctggcc cttgcccagg tcatgatggg
     1201 gcagtacgac aacgccatca gcaactacaa ggcggcgctc cgcctcaagc cctcgctgat
     1261 cgaggccagg atggggctcg cgcatgccta ctgggaaaag ggtgactacg agaacgccta
     1321 cagcgtgtac atgcagctct accaggaagg cgtcgtggcg ggctcgagac acgctcccac
     1381 catgtacttc aggctcgggt acatgacatg gaaatatggc gatccccgtg acgcgagggc
     1441 cttcctggca gccgccgagc cgggtctgag aggggacctg ttcaagctct cggaactcgc
     1501 aggcgtgtac acgagcatga acgacttcag aaacgcatac cgcgtctacc gcaccgtcta
     1561 cgaccgactc gaggaagggg aacccaaggc caagctcgcc aggctcctct ccgccctgga
     1621 aaagaagatg aggcagtgag ggcgacgggt ccgaccgctc cgccggggcg acgcgtggcg
     1681 gcatctcggt gggcagaggg gcagggacgg acgga
//
LOCUS       DAOSCR010000592         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47034_length_1709_cov_2.610641, whole genome shotgun sequence.
ACCESSION   DAOSCR010000592 DAOSCR010000000
VERSION     DAOSCR010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47034_length_1709_cov_2.610641"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLT69_11860"
     CDS             <1..581
                     /locus_tag="PLT69_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15181.1"
                     /translation="SAIYFRARRDLYTPGALYRLDLATEKVTAVSFMAMSSFDVSPDG
                     SSIVCAAPMDEAAPTSKDCLWIMDASGAHARPLGNTSGASCPAFSPDGARVAYACPDK
                     ASLVTQIHILDLEGLRQQRVTSSPYPKNRPSWSPDGKILCCEAQVDTDGPAATVIFAV
                     NADGTDEGMLGCSSAPSTGGPARASCPSFSPR"
     assembly_gap    599..608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            757..>1709
                     /locus_tag="PLT69_11865"
     CDS             757..>1709
                     /locus_tag="PLT69_11865"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOJ15182.1"
                     /translation="MVYMAADNDLWSAGIADINEMEAVGSSIDVNVVVQAEFKRAYPE
                     GAPGNAVRGLVTPDDDLLAISSRLSDIGRKDMSDPETLLDFIAWAKESYPAKRYALVI
                     WNHGNGWKDGDARPLGSRGVILDDSSGSSLMSLPELAQALALSGVRLDLVDFDACYMA
                     MYEVAYELADLSGYLAFSEDTTPADGNPYTEILWGLSLNPDMDGRELAMLVVSEYRRC
                     YGTPPATRATKSAFDLSLAPLLHASITSLAGTIMSGSDRGADLIGLARESSVHCSGKR
                     YYHDLGSFLDALSSASTPGVVREAALRCRQTLQLAVIDSTS"
BASE COUNT          294 a          585 c          539 g          281 t
ORIGIN      
        1 catcggccat ctatttcagg gcgagaagag acctctacac ccctggcgcc ctctaccggc
       61 tcgacctcgc caccgagaag gtcacggccg tgagcttcat ggccatgagt tccttcgacg
      121 tctcgccgga cggttcgtcc atcgtctgcg cagcgcccat ggacgaggcc gcgcccacct
      181 cgaaagactg tctctggatc atggacgcat ccggtgcgca cgcgagaccg ctcggaaaca
      241 cgagcggggc ctcgtgtccc gccttctcgc ccgatggggc gagggtagcg tatgcctgcc
      301 ccgacaaggc gagcctcgtc acccagatcc acatcctcga cctggaaggg cttcggcagc
      361 aaagggtgac ctcgagcccg taccccaaga acaggccgtc atggtcgccc gacgggaaga
      421 tcctgtgctg cgaagcgcag gtggacacgg acggtcccgc agcgaccgtg atcttcgcgg
      481 tgaacgcgga cgggacggat gagggcatgc tcggatgctc gtcagcgccc tcgacgggcg
      541 gaccggcaag ggcgtcgtgc ccgtcgttct ccccgaggta gacgggccgt gaaccaccnn
      601 nnnnnnnngg tggacatccc gcacttaagc cccacgcacc cggggcgccg cctcgcctct
      661 ttcttccttg cgctcgcaac gctcgctttc gcgtgggggt gttcttcctc gggcggaaca
      721 ggctcagacg ccgccaaaga ggcggcctgg accgtcatgg tctacatggc cgcggacaac
      781 gacctctgga gtgcgggcat cgccgacatc aacgagatgg aggccgtggg ctcgtcgatc
      841 gacgtgaacg tggtggtgca ggcggagttc aagagggcat acccggaagg cgccccggga
      901 aacgccgtca gggggctggt gacccctgat gacgacctgc tcgcgatctc gagcaggctc
      961 tccgacatcg gcaggaagga catgagcgac cccgagaccc tcctcgactt catcgcctgg
     1021 gcgaaggaat cgtaccctgc caagaggtac gccctcgtca tctggaacca cggaaacggc
     1081 tggaaggatg gcgacgctcg ccccctggga tccaggggcg tgatcctgga cgactcctcg
     1141 gggtcatccc tcatgtcgct gcctgaactg gcgcaggcgc tggccctcag cggcgtccgc
     1201 ctcgacctcg tcgacttcga cgcgtgctac atggccatgt acgaggtggc gtacgaactc
     1261 gccgacttga gcggctatct cgcgttctcg gaggatacga ccccggcgga cggcaacccc
     1321 tacacggaga tcctctgggg tctttcgctg aacccggaca tggacgggcg agagctggcc
     1381 atgctcgtgg tctcggaata ccggaggtgc tacggaacgc ccccggcgac gagggcgacc
     1441 aagtccgcct tcgacctgtc cctggccccc ctcctccatg cgtcgatcac ctcccttgcc
     1501 ggcacgatca tgtccgggtc cgacagggga gcagacctca tcggcctcgc ccgggaaagt
     1561 tccgtccact gctcgggcaa gagatactac catgacctgg gctcgttcct ggacgccctc
     1621 tcctctgcct ccacgccggg tgtcgtccgg gaagcggccc tcaggtgcag gcagaccctg
     1681 cagctcgccg tgatcgacag cacctcgat
//
LOCUS       DAOSCR010000593         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47157_length_1706_cov_3.513022, whole genome shotgun sequence.
ACCESSION   DAOSCR010000593 DAOSCR010000000
VERSION     DAOSCR010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47157_length_1706_cov_3.513022"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(582..>1706)
                     /locus_tag="PLT69_11870"
     CDS             complement(582..>1706)
                     /locus_tag="PLT69_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOJ15183.1"
                     /translation="RCDPESSNMGYFGWPMSASDTMTRLYTGQGSLLTRQMEFFPSHF
                     PHDSWIVDSALELFRREDPDVAYILLAQCDDAGHCLGNAPDPDEFVEDQEAPSLPDGC
                     EERPAYRLVSRRNRLLYKEAILDAVREVDVQFGRLVKGLGELGVLDDAVVVVLSDHGS
                     VNHLWSPDFSSTDFVGILKDAGILKPRGALDGMDMYAFSVSSYGVLYWRGDKRLVGAA
                     KKVLESHRARNPESGSLECPWWVLDRKDMMHGLEGVCPPGELYHPFYAGPQGEGMVMW
                     PDLFVFAKNGWQIPVYNGHVPNVGIGAPSWAPPWRVYNGGHGSVDTTPILAAVRVPGG
                     RRGVNDRPVLISDLAVTAASLLGVPLEGKKTGRDLAGELS"
BASE COUNT          266 a          604 c          561 g          275 t
ORIGIN      
        1 ctcgatcctc ttgaacggtg tatccatgtt cccagggata atacgaggcg cgcatcgaat
       61 gcaaggggag cgcttgcatc gttttggcag gcgaccttcc cgcatgttct cgtgaaagac
      121 ccctcgggag gcgtccggaa gggcaccatc gccgcgctct tgttgataca ccggagaaac
      181 gcgccagtta cgggtgggcc tggagccccc gcctggtccc ccgagatcgg cccgggggcc
      241 cgagcggtcc cggcccgctg tcaccccggc acgcccaggc ccatgcgggg acagggggaa
      301 ctaccccatg tccccctggc gtgacccggg aagcaagacc gtgggccttg ccttcatcga
      361 cgtctccacg ggggcgtggt gcgcggcgga acccgacgga gaggactggc tcccacccgg
      421 gatcgcgagg gcggcgccca tgcgggtcgt ccattccaca cagtacagcc cctcaaggcg
      481 accacccggg gcgcggggcg acccaggccg gatcgccctc acgcaccgag agcctgcgtc
      541 gagaccggag ccggctgccg cccggaccct tccgggagaa ctcacgagag ctcgcctgcc
      601 aggtccctgc ccgtcttttt cccctcgaga ggcacgccca gcagggaggc ggcggtcacc
      661 gcgaggtcgc tgatgagcac aggcctgtcg ttgacgcccc ttcttccccc gggcaccctc
      721 accgcggcca gtatgggcgt cgtgtccacg gagccgtgcc cgccgttgta cacccgccag
      781 ggcggggccc atgacggcgc gccgatgccc acgttgggca cgtggccgtt gtacacgggg
      841 atctgccagc cgttcttggc gaagacgaag aggtcgggcc acatcaccat gccctcgccc
      901 tgagggcctg catagaaggg gtgatagagc tctcccggcg ggcagacgcc ctcgaggccg
      961 tgcatcatgt ccttgcggtc gagcacccac caggggcact cgagggagcc gctctccggg
     1021 tttctggccc ggtgcgactc gagcaccttc ttggcggccc ccacgagacg cttgtccccg
     1081 cgccagtaca acacgccgta gctgctcacg ctgaaggcgt acatgtccat cccgtcgagc
     1141 gcgccgcgcg gcttgaggat ccccgcgtcc ttgaggatgc ccacgaagtc agtcgaggag
     1201 aagtcggggc tccacaggtg attcaccgac ccgtggtcgc tcaacacgac gacgaccgca
     1261 tcgtcgagca cgcccagttc gcccaggccc ttcacgagtc gtccgaactg gacgtccacc
     1321 tcccggacgg cgtccaggat ggcctccttg tagaggagcc tgttcctcct gctgacgagc
     1381 ctgtatgcgg gcctctcctc gcagccgtcg ggcagggacg gggcctcttg gtcctcgacg
     1441 aactcgtccg ggtccggcgc gttgcccagg cagtgtcccg cgtcgtcgca ctgcgccagg
     1501 aggatgtagg ccacgtcggg gtcctcgcgc ctgaacagct ccagcgcgga gtccacgatc
     1561 cagctgtcgt gggggaagtg gctcgggaag aactccatct gcctcgtgag gaggctgccc
     1621 tggcccgtgt acaggcgtgt catcgtgtcg ctggcgctca tgggccaccc gaagtatccc
     1681 atgttcgacg actctgggtc gcaccg
//
LOCUS       DAOSCR010000594         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47159_length_1706_cov_3.300424, whole genome shotgun sequence.
ACCESSION   DAOSCR010000594 DAOSCR010000000
VERSION     DAOSCR010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47159_length_1706_cov_3.300424"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLT69_11875"
     CDS             <1..1091
                     /locus_tag="PLT69_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ15184.1"
                     /translation="RAGARIALCAPTGRAARRMSEATRFEASTIHRLLEYNPGSSTFL
                     KNENRPIEADLVVVDECSMVDITLMHHLLKAVRPGTTLVLVGDVDQLPSVGPGDCLRD
                     VISSVVAEVVRLDTIYRQEKTSGIVDNCHRINKGAMPVVSTDLSGDFTFVPEEDPERI
                     LGLVVEHVTSRLPSMHGLDPLSDIQVLSPMHKGVLGVDNLNAVLQRAVNPSGRPFKKR
                     HGEFRLGDKVMQTRNNYELEVFNGDIGRLVDMDERDGSCTVDFEDRLVAYSQADALDL
                     VVAYATTIHKAQGSEYPCVVMPVHTQHALMLARNLIYTAVSRARKIVVTIGSERALRY
                     GIENTRSSRRYTLLARRLAQEASSPATP"
     gene            1185..>1706
                     /gene="kdsA"
                     /locus_tag="PLT69_11880"
     CDS             1185..>1706
                     /gene="kdsA"
                     /locus_tag="PLT69_11880"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOJ15185.1"
                     /translation="MRTVQVRDVTIGGGTLVLIAGPCVIESRDHCLFLAERIAETARR
                     RGVPCIFKASFDKANRTSASSFRGPGLTEGLAVLAEVKAATGLPVVSDIHEPGQAPQA
                     SEVLEMIQIPAFLCRQTDLLAAAGRTGLPVNIKKGQFLAPWDMAHAVEKVRLAGNDSV
                     VLTERGTSLGYNNL"
BASE COUNT          321 a          590 c          541 g          254 t
ORIGIN      
        1 acagggcggg cgcccgtata gccctgtgcg cgcccacggg cagggccgcg aggcggatga
       61 gcgaggccac gagattcgag gcctcgacca tccaccgtct cctcgaatac aaccccggtt
      121 catcgacctt cctcaagaac gagaacaggc ccattgaggc ggacctggtg gtcgtcgacg
      181 agtgctcgat ggtggacata acgctcatgc accacctcct gaaggccgtg agacccggca
      241 cgaccctcgt cctcgtcggc gacgtcgacc agctcccgtc ggtcggcccg ggcgactgcc
      301 tccgggacgt gatctcatcg gtcgtggccg aggtggtgag gctcgacacc atctaccggc
      361 aggaaaagac gagcggcatc gtggacaact gccaccggat caacaagggc gccatgccgg
      421 tcgtgtcgac ggacctctcg ggcgacttca cgttcgtccc ggaggaggat cccgaacgca
      481 tcctcgggct cgtggtggag cacgtgacct ctcgactacc gtccatgcac ggcctcgacc
      541 cgctctcgga catccaggtg ctgagcccca tgcacaaggg ggtcctgggc gtggacaacc
      601 tgaacgccgt gctgcagcgg gccgtgaacc cttcgggcag gccattcaag aagcgccatg
      661 gggagttcag gctcggcgac aaggtcatgc agaccaggaa caactacgag ctcgaggtct
      721 tcaacgggga cataggcagg ctcgtggaca tggacgagcg cgacggatcg tgtacggtgg
      781 acttcgaaga caggctcgtc gcatactcgc aggcggacgc cctcgacctg gtcgtcgcgt
      841 acgccaccac catccacaag gcccagggat cggagtaccc gtgcgtcgtc atgccggtgc
      901 acacccagca cgcgctcatg ctggccagga atctcatcta caccgccgtg tcccgcgcca
      961 ggaagatcgt cgtgaccatc ggttcagagc gcgccttgag gtacggcatc gagaacacca
     1021 ggagcagcag gcgctacacg ctgctggccc ggcgcctcgc gcaagaggcc tcatcccccg
     1081 ccacaccata ggcgttccca gtgccccgcg gcacagggaa gcggaccggg gccgcttgac
     1141 acccccaccc ccatacgtta catctgctcc cagggagtat acgcatgcgc accgtacagg
     1201 taagggacgt caccatcggg ggcggcacgc tcgtgctcat cgccgggcca tgcgtgatcg
     1261 agtccaggga ccactgcctc ttcctggccg aacgcatagc cgagacggct cgcagaaggg
     1321 gcgtcccttg catcttcaag gcctccttcg acaaggcgaa ccgcacctca gcatcgtcgt
     1381 tcagggggcc cgggctcacc gaaggcctcg ccgtcctcgc cgaggtgaag gcggccaccg
     1441 gcctgcccgt ggtctcggac atccacgagc ccggccaggc cccgcaggca tccgaggtcc
     1501 tcgaaatgat ccagatccct gccttcctct gccgccagac ggacctgctc gccgcggccg
     1561 ggaggacggg gctgcccgtc aacatcaaga agggccagtt cctggcgccc tgggacatgg
     1621 cgcacgcggt ggagaaggtg aggctcgcgg ggaacgactc ggtggtcctc accgagcgcg
     1681 gcacgtccct cgggtacaac aacctg
//
LOCUS       DAOSCR010000595         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47311_length_1703_cov_1.398058, whole genome shotgun sequence.
ACCESSION   DAOSCR010000595 DAOSCR010000000
VERSION     DAOSCR010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47311_length_1703_cov_1.398058"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..306
                     /locus_tag="PLT69_11885"
     CDS             61..306
                     /locus_tag="PLT69_11885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOJ15186.1"
                     /translation="MAEQTARAIENARCILNACGLGLENGVKTEVFLRDMDDFAGMNA
                     AYATFFTAEPRPARTTVEVSRLPRDVLVEISCVAYRG"
     gene            complement(346..1110)
                     /locus_tag="PLT69_11890"
     CDS             complement(346..1110)
                     /locus_tag="PLT69_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOJ15187.1"
                     /translation="MALLVALAGLLVGSFLNVCIYRIPRGESVVWPSSRCPACSTPIR
                     AWDNVPVLSYLVLGGRCRACGHPISVRYPLVELLSALLAVGMLYRFGPGPAFVIYYAW
                     SCVLVVITFKDIDHRIIPDRFSLGGMAAGIALVPFMPISYTDALAGLALGGGLLIAVI
                     YAYYLLTKREGMGGGDVKLLAMIGVYTGWKGVLFTIFAASLAGTAFGIPWALSRREGG
                     SAMKAAVPFGPFLALGALVYVFFGDRIIEWYFGFLA"
     gene            complement(1112..1537)
                     /locus_tag="PLT69_11895"
     CDS             complement(1112..1537)
                     /locus_tag="PLT69_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15188.1"
                     /translation="MKIDPNMIIGAVTPQAGRPAKGAGGSFEEILGSVQEKRTEGAVR
                     IGGPLPGMPVDPVALNALKAGEEALDKLETYRRALENPALGLKDLEPMVVDLSRMKDR
                     MEEAVSFLSDADPLRAVMSEMAGVLGAEVARFRRGDLVG"
BASE COUNT          321 a          572 c          545 g          265 t
ORIGIN      
        1 gtgttcgtgt caggatcgat ccccctcgat cccgcaaccg gcgaggtcgt gtaggggggt
       61 atcgcggaac agaccgctcg cgccatcgag aacgcgagat gcatcctgaa cgcttgcggg
      121 ctcggcctcg agaacggcgt gaagaccgag gtgttcctca gggacatgga cgactttgcg
      181 ggcatgaacg cggcatacgc gacattcttc acggcggagc cgaggcccgc gcgcacgacc
      241 gtggaagtct ccaggctccc cagggacgtg ctcgtggaga tctcgtgcgt ggcctaccgt
      301 ggatgacgcg ccggtctcag gcgccccgtg aggcaccaga cctgatcatg cgaggaaacc
      361 gaagtaccac tcgatgatcc ggtcgccgaa gaacacgtaa acgagcgcgc caagcgccag
      421 gaagggcccg aagggcaccg cggccttcat ggcgctgccc ccttccctcc tggacaaggc
      481 ccaggggatg ccgaaggccg tacctgcgag cgaggcggcg aagatggtga agaggacccc
      541 cttccagccg gtgtacaccc ctatcatggc caggagcttc acgtcccccc cgcccatccc
      601 ctctctcttc gtgagcaggt agtacgcgta gatcacggcg atgagaagcc ccccgccgag
      661 cgcgaggccc gccagggcgt cggtgtaaga gatcggcatg aaggggacca gcgcgatccc
      721 cgccgccatg ccgcccaggg agaacctgtc cgggatgatg cggtggtcta tgtctttgaa
      781 ggtgatcacg acgagcacgc acgaccatgc gtagtagatg acgaaggcgg ggccgggccc
      841 gaaccggtac agcatgccca cggcgagcag tgcgctcagg agctcgacca ggggataccg
      901 cacggaaatg ggatgcccgc atgcacgaca cctcccgccg aggaccaggt agctcaagac
      961 cgggacgttg tcccatgccc gtatgggggt cgagcaggcg gggcagcgcg acgagggcca
     1021 gacgacggac tcccctctcg gaatgcggta aatgcagacg ttcaggaagc tccccacgag
     1081 gagacccgca agcgctacga ggagggccat gtcagcctac caggtccccc ctcctgaaac
     1141 gggccacctc tgcccccagg acgccggcca tctcggacat gaccgcccgc agcgggtcgg
     1201 catcgctcag gaacgagacg gcttcttcca tccggtcctt catccgggaa aggtccacga
     1261 ccatgggttc gaggtccttg agccccaagg cggggttctc cagggcccgc ctgtaggtct
     1321 ccagcttgtc gagggcttcc tccccggcct tcagtgcgtt gagggcgacc gggtccaccg
     1381 gcatgccggg aaggggcccg ccgatcctta cggcaccctc ggtgcgcttc tcctggacac
     1441 tgcccaggat ctcctcgaaa gacccccccg cccctttggc gggcctgccc gcctgcggcg
     1501 taaccgcgcc gatgatcatg ttcgggtcta tcttcatctt tcctccctcc ccgttcaaag
     1561 caaggacggt gccagtcccc acctcccttg tcggcatcgg gtcgatccct cgtaagcggt
     1621 ggaagctttg acccgggagg ggaaaaaatg tccggcctca agccttctct cacggcgaag
     1681 agggagcgag cctctcgatg aag
//
LOCUS       DAOSCR010000596         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47324_length_1702_cov_3.241044, whole genome shotgun sequence.
ACCESSION   DAOSCR010000596 DAOSCR010000000
VERSION     DAOSCR010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47324_length_1702_cov_3.241044"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..669)
                     /locus_tag="PLT69_11900"
     CDS             complement(64..669)
                     /locus_tag="PLT69_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15189.1"
                     /translation="MSDGKDVNVTIKIADAGPGGWIAYSIATWIAWAFLCGFVGPKAL
                     LLMACISLACTIPYLGAAITQLKLGNVAGGVTWLYFGAFFAFCSALTYFVSYFAPIYG
                     WELDAKVLGFEWAVLGVTLILTTPIFLKYAPASASISVIGADVGIAFLVLIYFGYASP
                     AVMQISGWGFFVAGLFGIVMTVGGILGAAGMKFPMGAPLIK"
     gene            complement(828..1046)
                     /locus_tag="PLT69_11905"
     CDS             complement(828..1046)
                     /locus_tag="PLT69_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOJ15190.1"
                     /translation="MDHKVTATMPGLVARVVVNVGEKVAEGQEVAVINCMKTEISVKA
                     DKPGAVKEILVKEWDEMQVGDPMVVLEI"
     gene            complement(1077..>1702)
                     /locus_tag="PLT69_11910"
     CDS             complement(1077..>1702)
                     /locus_tag="PLT69_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HOJ15191.1"
                     /translation="DVPPYLGSKSTCIFGSYGGFEGRKLAPGDVLKFGKPARALDTLA
                     GRKLKKGVKPEFARTWELRAIPGPNTCPDYVTKEGMDYLYENEHKIQHTSNRSAYRIE
                     PVPEYFFARADGGVGGSHPSNIVDHGYAIRGTMNVCGNTPILLIADGPTLGGYINVLH
                     VINADLWKIGQGTPARDKFTFVYCTREQAIEERKKQRALFTEDNVTK"
BASE COUNT          313 a          556 c          495 g          338 t
ORIGIN      
        1 gtccagcgag caccctgccc ccatgcaccc aggcatgaac ctcgagtctc ctgtcggtcc
       61 cggtcatttg atgagcggcg cacccatggg gaacttcatg cctgctgcac cgagaattcc
      121 tcccaccgtc atcacgatgc cgaacagccc ggccacgaag aacccccacc cgctgatctg
      181 catgaccgca ggcgacgcat agccgaaata gatgagcacg aggaacgcga tgcccacatc
      241 ggctcctatg accgagatcg acgcggatgc cggcgcgtac ttcaggaaga tcggggtggt
      301 gagtatgagg gtcacgccga gtaccgccca ctcgaacccg agcaccttcg catccagctc
      361 ccacccgtat atcggggcga aataactgac aaaataggta agggccgagc aaaaggcgaa
      421 gaacgccccg aagtacaacc aggtgacgcc acccgccacg ttcccgagct tgagctgggt
      481 aatggccgct ccgagatacg ggatcgtgca cgcaagcgat atgcaggcca tgagaaggag
      541 cgccttcggc ccgacgaaac cgcagaggaa tgcccaggcg atccacgtgg cgatggagta
      601 ggctatccaa ccgccaggcc ctgcatcggc gatctttata gtgacgttga catcttttcc
      661 atctgacatg aacgcacctc ctgtcggtat atgaggtcac ggggcagacg gaggtcccgg
      721 ggacagagat cgagggttct cccccgcatc cctccatcct caggtctcag cgccgcccgc
      781 atgaccggtc tcctgtccgt cctttcgtca tcatccaggc aggggaatca gatctcgagg
      841 acgaccatgg ggtctcccac ctgcatctcg tcccactcct tgacgaggat ctccttgacc
      901 gcgccgggct tgtccgcctt gacgcttatc tcggtcttca tgcagttgat caccgcgacc
      961 tcctgtccct ccgccacctt ctcacccacg ttcacgacca cccgggccac gaggccgggc
     1021 atggtagcag taaccttgtg atccatatgt cctccttgtg ctggtctgtc gctgcgtcac
     1081 ttggtgacgt tgtcctccgt gaagagcgcg cgctgctttt tccgctcctc gatggcctgt
     1141 tccctcgtgc agtagacgaa cgtgaacttg tctctcgcgg gggtcccctg cccgatcttc
     1201 cagaggtcgg cgtttatgac gtggagcacg ttgatgtaac cgccgagcgt cgggccgtct
     1261 gctatgagga ggatcggcgt gtttccgcac acgttcatgg tcccgcggat ggcgtagccg
     1321 tggtcgacga tgttcgaggg gtgcgacccg cccacaccgc cgtccgcgcg tgcgaagaag
     1381 tattcgggca ccggctcgat gcggtacgcc gagcggttcg acgtgtgctg tatcttgtgc
     1441 tcgttctcgt agagataatc catgccctcc ttggtgacgt agtccgggca ggtgttcggc
     1501 cccgggatgg ccctgagttc ccaggtccga gcgaactcgg gtttgacccc tttcttgagc
     1561 ttcctgcctg caagcgtatc gagcgcgcgg gccggcttgc cgaacttcag gacgtcgccg
     1621 ggggcgagct tcctcccctc gaacccgccg tagctgccga atatgcaggt ggacttgctc
     1681 ccgagatacg gcggcacatc ga
//
LOCUS       DAOSCR010000597         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47327_length_1702_cov_3.170006, whole genome shotgun sequence.
ACCESSION   DAOSCR010000597 DAOSCR010000000
VERSION     DAOSCR010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47327_length_1702_cov_3.170006"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1613
                     /locus_tag="PLT69_11915"
     CDS             288..1613
                     /locus_tag="PLT69_11915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOJ15192.1"
                     /translation="MRICMIGSGYVGLVTGACLAEMGNDVVCVDENADKISMLNRGEI
                     PIYEPGLKEIVNRNASEGRLRFTTDLEEAVGKSLLCFICVGTPQDKDGSADLSAVLEV
                     ARRLGDIMDGYRIVVDKSTVPVGTGEMVRATIRKRLEERGLGHVEFDVVANPEFLKEG
                     NAVQDFMRPDRVVIGTDNVRTAEILKELYSPFVRTTNNPILVMDIRSAELTKYASNCF
                     LAAKVTFMNELAILCDRLGANIDLVREGMGTDERIGPRFLLAGPGFGGSCFPKDVQAL
                     IATARSNNHTLEICEATWRANQRQKRYVADKVISCFAGEDLSKVTAAVWGLTFKPKTD
                     DVRESPAIDIINYLLEHGITVKAYDPQGMPNARALFGDRITYAQDAYTALAQADCLII
                     ITEWNQFKSPDIQRMKSLMKRPIVVDARNILQPNEMRAEGFAYHGIGRL"
BASE COUNT          368 a          569 c          484 g          281 t
ORIGIN      
        1 cgaggtagct cagtcggtag agcaggggac tgaaaatccc cgtgtcggcg gttcaattcc
       61 gtccctcggc accactcggc cgtaccgaca ccccctgaga acaccagaga catcaggcca
      121 gtcaacacat ctcgtatccg actccgaccc acacttgatt ccatagaaac cttaggacac
      181 cctgccgacg cgcctgatcg tgagacccgc acgagcacgg cacttgtgaa cctcgggaaa
      241 aatcggctat ctgataccca cgaactcccc caccacaagg agccgccatg agaatttgca
      301 tgatcggcag cgggtacgtc ggcctcgtga caggcgcgtg cctcgctgag atgggcaacg
      361 acgttgtctg cgtggatgag aacgctgaca agatctccat gctcaaccgg ggcgaaatcc
      421 ccatctacga gcccggcctg aaggagatcg tgaaccgaaa cgcctcggag gggcggctcc
      481 gcttcaccac ggacctcgag gaagccgtgg gcaagagcct gctctgcttc atctgcgtag
      541 ggacgccgca ggacaaggac ggctcggccg acctttccgc ggtgctcgag gttgcccgcc
      601 gtctcggcga catcatggac ggctaccgca tcgtcgtgga caagtccacc gtgcccgtgg
      661 gcacgggcga gatggtgcgg gccacgatca gaaaaaggct cgaggagcgc ggcctcggcc
      721 atgtcgagtt cgacgtggtc gcgaaccccg agttcctcaa ggaaggcaac gccgtgcagg
      781 acttcatgag gccggaccgc gtcgtcatcg gcacggacaa cgtccgcacc gccgagatcc
      841 tgaaagagct ctacagcccc ttcgtgcgca ccaccaacaa ccccatcctc gtcatggaca
      901 tacgcagcgc ggagctcacc aagtacgcgt cgaactgctt ccttgccgcg aaggtcacgt
      961 tcatgaacga gctcgcgatc ctctgcgaca ggctcggcgc caacatcgac ctcgtgcgcg
     1021 agggcatggg gacggacgag cgcatcggcc cgcgctttct gctcgccggc ccgggattcg
     1081 gcggctcgtg ctttcccaag gacgtccagg cgctcatcgc cacggcgagg tcaaacaacc
     1141 acaccctcga gatctgcgag gcgacctggc gcgccaacca gcgccagaag cgctacgtgg
     1201 cggacaaggt catctcctgc ttcgcgggcg aagacctctc caaggtcacg gccgccgtct
     1261 ggggcctcac cttcaagccc aagacagacg acgtgcgcga atccccggcc atcgacatca
     1321 tcaattacct gctcgaacac ggcatcaccg tcaaggccta cgacccgcag ggcatgccga
     1381 acgcccgcgc cctcttcggc gaccgcatca cctacgccca ggacgcctat accgccctcg
     1441 cccaggccga ctgcctgatc atcatcaccg agtggaacca gttcaagagc cctgacatcc
     1501 agcgcatgaa atccctcatg aaacgcccca tcgtcgtgga cgcgagaaac atcctccagc
     1561 ccaacgagat gcgcgccgag gggttcgcat accatgggat cgggaggctg tgaggaggct
     1621 ctcatcaagt ttcttgatta agtttaatta aggttcatga caactttagg gggttagatc
     1681 gtaacatttt gaggagcagt tt
//
LOCUS       DAOSCR010000598         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47435_length_1700_cov_2.282067, whole genome shotgun sequence.
ACCESSION   DAOSCR010000598 DAOSCR010000000
VERSION     DAOSCR010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47435_length_1700_cov_2.282067"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PLT69_11920"
     CDS             <1..1359
                     /locus_tag="PLT69_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ15193.1"
                     /translation="ALSSAVTFYAQAKGGSVRTLDEIPICARVANSVVSYAMYLAKTF
                     VPMNLAAFYPFPPRIEPAAVIVSGAALALVSALAALWWKKRPYLATGWLWYLGTLVPV
                     IGLVQVGKQSMADRYTYVPLVGVFVMLTWGLADLAGGGARAKKAKTAAAVAVVGLCMV
                     LTYRQVGYWENGETLFRRMISSTKNNSIGHYNLGCVLQAGKDYDEAMTHYEEALRIDP
                     KFLRPRYNLAGILLSQGRYDEAIGHYKTSLEILPGQAKVLNNLGTAYFKKGDLARAIR
                     SFEEACSSDPRYKEARKNLDEALRARETLVLRLEGELERARRNTRDVQARLEAADTCR
                     SLGRYDEAEELYRECLALDGSSERALKGLAVLARDRSRWDEALSWLERLAAARPSDPL
                     PLYDIACIHALAGRREEAVESLRASVRKGFSDFSLLARDPDLASIRDHPYVASLLGSG
                     QK"
     gene            1477..>1700
                     /locus_tag="PLT69_11925"
     CDS             1477..>1700
                     /locus_tag="PLT69_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOJ15194.1"
                     /translation="MTRRRKIILSLAAGIVFSAAALRVSFANVPMGDLAAALSSVDPL
                     WVAVSIVLGYASYIARTLRWLVILRPVKKVP"
BASE COUNT          292 a          571 c          565 g          272 t
ORIGIN      
        1 gcgctctctt cggccgtcac cttctacgcc caggcaaaag ggggctcggt caggaccctg
       61 gacgagatcc ccatctgcgc gagggtcgcc aactcggtgg tgtcctacgc catgtacctc
      121 gccaagacct tcgtgccgat gaatctcgca gccttctacc ccttcccccc gaggatcgag
      181 cctgcggcgg tcatcgtctc gggtgcggcc ctcgccctcg tgagcgcatt ggcggccctg
      241 tggtggaaga agaggccgta cctcgctacc gggtggctct ggtacctggg caccctcgtg
      301 ccggtcatcg ggctcgtgca ggtgggcaaa cagtccatgg cggaccgtta cacctacgtc
      361 ccgctcgtgg gcgtgttcgt catgctcacc tgggggcttg cggacctcgc ggggggaggc
      421 gcccgggcga agaaggccaa gacggccgcg gcggtggcgg tcgtgggcct gtgcatggtc
      481 ctcacctaca ggcaggtggg gtactgggaa aacggcgaga ccctcttccg gaggatgatc
      541 tcgtccacaa aaaacaactc catcggccac tacaacctgg ggtgcgtgct ccaggcggga
      601 aaggactacg acgaggccat gacgcactac gaggaggcgc tgaggataga ccccaagttc
      661 ctgaggccgc gctacaacct cgcaggcatc ctcctttcac agggcaggta cgacgaggcc
      721 atcgggcact acaagacctc cctcgagatc ctgcccggcc aggcaaaggt cctcaacaac
      781 ctcggcacgg cgtacttcaa gaagggggac ctcgccaggg ccatacggtc gttcgaggag
      841 gcctgttcga gcgacccccg gtacaaggag gcacggaaga acctcgatga ggcgctcagg
      901 gcgagagaga ccctcgtact ccgcctcgag ggcgagctcg agcgggcccg ccggaacaca
      961 cgcgacgtgc aggcgcgcct ggaagccgcg gacacgtgcc gatcactggg gaggtacgac
     1021 gaggctgaag agctctaccg ggaatgtctc gcgctggacg gctcctccga aagggccctg
     1081 aaggggctgg ccgtcctggc gagggaccgc tcacgctggg acgaggccct ctcctggctt
     1141 gagaggctcg ctgccgcgag gccttcggac ccgctgcccc tctacgacat cgcctgcatc
     1201 catgcgcttg cgggccggag ggaagaggcg gtggagagcc tccgcgcgtc cgtgaggaag
     1261 gggttcagcg acttctccct cctcgcgcgc gaccctgacc tcgcgtccat cagggaccac
     1321 ccctacgtgg cgtccctgct cggaagcggg caaaagtgag accgagggca gccctggaca
     1381 cgcccgtgtt gcaaccacgg gcgcctttcg gctatggtgc cagcagcaca tgacgcctgc
     1441 tcggcacggc tgcgccgcag gccatcgaag gagaccatga ccaggaggcg caagatcatc
     1501 ctctccctgg cggccggcat cgtgttctcg gccgcggcgc tgcgcgtctc tttcgcgaac
     1561 gtgcccatgg gagacctggc ggcggcgctg tcgtccgtcg accccctgtg ggtggccgtg
     1621 tccatcgtcc tcggctatgc cagctacatc gccaggaccc tcaggtggct cgtgatcctc
     1681 aggcccgtga agaaggtccc
//
LOCUS       DAOSCR010000599         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47439_length_1700_cov_2.080851, whole genome shotgun sequence.
ACCESSION   DAOSCR010000599 DAOSCR010000000
VERSION     DAOSCR010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47439_length_1700_cov_2.080851"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1056
                     /locus_tag="PLT69_11930"
     CDS             142..1056
                     /locus_tag="PLT69_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ15195.1"
                     /translation="MERQTSTGRTFTSDLLLLTTAAIWGLAFVAQRAGMEHVGPFGFN
                     GVRFMLGAMVLLPLLARRSASRREQGQGEDPRWRVPVGGGLAAGLVLFAGASFQQVAM
                     VYTQAANAGFITGLYVVIVPVIGILIGHRTNAGTWVGAVLAAVGMYLLSVSGPLEFAY
                     GDMLVLVGAVFWACHVHIIGYLSPKTDALAVSFIQYTVCACLSLAVSFLLEANTLDGY
                     LGAAVPILYGGVMSVGVAYTLQVVAQKKAKPSHAAVILCLEAVFAALGGWVLLGETLT
                     GKGLAGCALMFAGMLSSQLMNAAEDKGA"
     gene            complement(1350..>1700)
                     /locus_tag="PLT69_11935"
     CDS             complement(1350..>1700)
                     /locus_tag="PLT69_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ15196.1"
                     /translation="FAVPRDGFLELMDEKAFRDEFIAMLMRRQRYLAGRILYLAGFDV
                     EERFFRFLIEHYGTGPVYRVDMAKKDLAAAIGTIPETLSRLVNRLKDVGVISWEGKTL
                     EVDEHYLGHFLDTP"
BASE COUNT          265 a          563 c          587 g          285 t
ORIGIN      
        1 ccgcggacgg tcgcttccac gggtatgccc gcggcacgag cacgcgcctt tgtccttgac
       61 tcggccgagc ccggccgggt aatccgcagg gaacacgacg aggcgaaccc ccgccgaggc
      121 ggggagacga ggcgcgacac catggaaagg cagacatcga caggcaggac cttcacttcc
      181 gacctcctcc tcctgaccac cgcggccatc tggggcctcg ccttcgtggc gcagcgcgcg
      241 ggcatggagc acgtggggcc cttcggcttc aacggcgtcc gtttcatgct gggcgcgatg
      301 gtgctcctgc cccttctggc gaggaggtcc gcgtcgcggc gggagcaggg tcagggtgaa
      361 gacccgcgat ggagggtccc ggtgggaggc gggttggctg cagggctcgt cctgttcgcc
      421 ggcgcctcgt tccagcaggt cgccatggtc tacacgcagg cggccaacgc cggtttcatc
      481 acgggcctgt acgtggtcat cgtgcccgtc atcggcatcc tgatcggcca caggacgaat
      541 gcagggacgt gggtgggggc ggtgctcgcg gcggtgggca tgtacctcct gagcgtctcg
      601 ggcccgctcg agttcgccta cggcgacatg ctcgtcctcg tgggggcggt cttctgggcg
      661 tgccacgtgc acatcatcgg gtacctctcc ccgaagaccg acgcgctcgc cgtctcgttc
      721 atccagtaca cggtgtgcgc ctgcctgagc cttgcggtgt cgttcctcct cgaggcgaac
      781 accctcgacg ggtacctcgg cgcagccgtc cccatcctgt acggcggcgt catgtcggtg
      841 ggcgtggcgt acaccctcca ggtggtggca cagaagaagg cgaagccctc gcacgcggcc
      901 gtcatcctgt gcctcgaggc cgtgttcgcg gccctcggcg ggtgggtgct cctcggtgag
      961 accctcacgg gaaagggcct tgcgggctgc gcgctcatgt tcgcgggcat gctctcctct
     1021 cagctcatga acgccgccga ggacaagggg gcctgaaccc agcctcgaga cggcccagcg
     1081 cagacccatg gaccggcgtg ttacggtgag ggggataagg gcggcccgag tgcaggctca
     1141 cacaggggcc ggcgaggcgt ccccacgcgc ctgtgtcctg ggaaaagccg tacaggcgag
     1201 cccgacagcg gcgcccggct acggggaatg catgtgcatc atacccgtct gccaggcttt
     1261 atccggggaa ctggacgccg aagggccctg gccgccttcc ctcgccgccc gcagaagagg
     1321 gcctcggggc ttgggacaag ccgaaggcct cagggcgtgt caaggaaatg accgaggtag
     1381 tgctcgtcga cctcgagggt ctttccctcc cacgagatca cgcccacgtc tttcaggcgg
     1441 ttgacgaggc gcgagagggt ctcggggatc gtgcctatgg ccgctgcgag gtccttcttc
     1501 gccatgtcca cccggtagac cgggccagtc ccatagtgct cgatgaggaa cctgaagaac
     1561 cgttcctcca cgtcgaagcc ggccaggtag aggatgcgac ccgcaaggta acgctgcctt
     1621 cgcatgagca tggcgatgaa ctcgtccctg aaggccttct cgtccatgag ttccaggaag
     1681 ccgtcgcgcg gcacggcaaa
//
LOCUS       DAOSCR010000600         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47741_length_1692_cov_3.740379, whole genome shotgun sequence.
ACCESSION   DAOSCR010000600 DAOSCR010000000
VERSION     DAOSCR010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47741_length_1692_cov_3.740379"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1126)
                     /locus_tag="PLT69_11940"
     CDS             complement(137..1126)
                     /locus_tag="PLT69_11940"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ15197.1"
                     /translation="MRPGFVTRLVNGPLFDPVLYVRHLNRRRALLFDCGRFSGLSNRE
                     ILALDAVFVTHTHMDHFMGFDCILRTVLHRGTPLHVYGPEGMGMRMAARLAAYTWNLT
                     SGYGLEVFVHEVLRDGVLLWCARAGEGFRLRRCGTEPREGTCIVETPRYRVDAVILDH
                     NVPCLGYVVSEPVHVHVRPEALKKHGFRTGPWIGDLKDKVAQGLLDRTLLVPTCRGEE
                     EWKVGDLSDWLFFTSPGHRIGYFTDFRASRENVERIRSAACGLDTLYIEAYYLSEREE
                     EAYLKAHVSARQAGVVARLTGASRVVPMHVSPRYHDRFGDIVSELERARLGGE"
     gene            complement(1143..>1692)
                     /locus_tag="PLT69_11945"
     CDS             complement(1143..>1692)
                     /locus_tag="PLT69_11945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOJ15198.1"
                     /translation="GEITRELHESIREIHGFLEPILTNLSEEDVQGISGRLTHVSSLV
                     KETSERTLDLLFARQERAVADNAQFRAIARSIISGDKKNALAGLKTLKDHNEELVAEL
                     MRISELQIHADLVDQIVRKVCRVIENIEERLVDLIRRHASAHEPMEPEGLSLMGPAVP
                     GLDTQAASSQDEVDSLLKSMGL"
BASE COUNT          278 a          576 c          536 g          302 t
ORIGIN      
        1 gggcgcaccc tcgggatggc accgagcccc gacgcgccgt atgcctcggg caggggaccc
       61 ccatccggat cgtaccgcct tcatgccgga gcgagctcca cccatggccc tcgggggatg
      121 ggggcgcggg caggactcac tcccccccca gcctcgccct ctccagttcg gagacgatat
      181 cgccgaagcg gtcgtggtac ctgggggaga cgtgcatggg gaccaccctc gaggcgccgg
      241 tcaggcgagc caccacaccc gcctgcctgg cgctcacgtg agccttcagg tacgcctcct
      301 cctcgcgctc cgagaggtag tacgcctcta tgtagagcgt gtcgagaccg caggcggccg
      361 agcgtatcct ctcgacgttt tcccgggagg ccctgaaatc ggtgaaatag ccgatcctgt
      421 gccccggcga tgtgaagaag agccagtccg acaggtcgcc caccttccat tcctcttccc
      481 cccggcacgt ggggacgagg agcgtccggt ccaacagccc ctgggccacc ttgtccttga
      541 gatcgcctat ccatggcccc gtcctgaaac catgcttctt gagcgcctcc ggccttacgt
      601 gtacgtgcac gggctcggag accacgtagc cgagacacgg cacgttgtga tcgaggatga
      661 ccgcgtccac gcggtagcgc ggcgtctcga caatacatgt gccctccctg ggctctgtgc
      721 cgcatcgtct caggcgaaaa ccctcgcccg ccctggcaca ccagaggagg acgccgtctc
      781 gaaggacctc gtggacgaac acctcgagcc cgtatccgga cgtgaggttc cacgtgtacg
      841 ccgcaaggcg ggccgccatg cgcatcccca taccctcggg cccgtagacg tggagggggg
      901 tgcccctgtg gagcacggtc ctcaggatgc agtcgaatcc catgaagtga tccatgtggg
      961 tatgggtcac gaacacggcg tccaaggcga ggatctctcg gttcgagagc ccggagaacc
     1021 gcccgcagtc gaacaggagg gcccgtctcc ggttgaggtg acgaacgtac agcaccgggt
     1081 cgaacagggg gccgttgacg agcctggtga cgaagcccgg cctcatgggg agagccgaac
     1141 cctcagagcc ccatggattt cagcaggctg tccacctcgt cctgggagga cgccgcctgg
     1201 gtatcgaggc ccggcaccgc gggtcccatc aggctgaggc cttcgggctc catgggttcg
     1261 tgggcgctcg cgtgccgcct gatcaggtcc accaggcgct cctcgatgtt ctctatgacc
     1321 ctgcagacct tcctcacgat ctggtccacg aggtcggcgt ggatctgcag ctcgctgatg
     1381 cgcatgagct cggcgacgag ttcctcgttg tggtccttga gcgtcttcag gcccgccagg
     1441 gcgttcttct tgtctcccga aatgatgctc ctggctatgg ccctgaactg ggcgttgtcc
     1501 gccacggccc tctcctgcct ggcgaacagg aggtcgagcg tcctctcgga ggtctccttg
     1561 acgagcgagg agacatgcgt gagcctgccc gagatcccct ggacgtcctc ctcggagagg
     1621 ttcgtgagga tcggctcgag aaacccatgg atctccctga tggactcgtg gagctccctc
     1681 gtgatctccc ct
//
LOCUS       DAOSCR010000601         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47757_length_1692_cov_3.031155, whole genome shotgun sequence.
ACCESSION   DAOSCR010000601 DAOSCR010000000
VERSION     DAOSCR010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47757_length_1692_cov_3.031155"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PLT69_11950"
     CDS             <1..1357
                     /locus_tag="PLT69_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOJ15199.1"
                     /translation="GIVFNGSKIGFSSLSMRRARGGEGLFEIRSLAAFAFRFLMVDKK
                     FSLKAYDLVDENLALVEFSYDYDLDGNVMHLEGRLQDGSLLTRVVTHGKVEEKVFAAC
                     GPVYPTSVLYLYPAFRGLATGKVYEMTVYDGETRSVSPARLEVLACEESDLFEGAGFK
                     VRVQLHSQEATAWIDRLGRPLLETAMGGVLVSGLEDDRRAREYLARTSLNKGDALLEM
                     SLVPTVTKIDRPREAETMEVVLVGVDSALSIPSDCRQVCTRQADGVHCRIERDAAPCP
                     CGGKDDREGGGSLRPSLEVQSGDARISALAESISAGRKGAREVASAVVAWMGANIRKE
                     AIDVFSALDVLERKKAECQGHALLYAALARASGIPTKVVSGLVYSAEHGGFLYHAWAE
                     SCLDGAWVAVDPTFGQLPADATHVKLVEGESPGDLIALAGFVGRISAGVLSCTYGGPE
                     P"
     gene            1423..>1692
                     /locus_tag="PLT69_11955"
     CDS             1423..>1692
                     /locus_tag="PLT69_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HOJ15200.1"
                     /translation="MLKKVLPALAARLIKTWCLTCRVRARINESAERNAVAEFGGAVY
                     ATWHQRMFYFFHDFGSRHVTMMISRSKDGDIANEVALRLGFLSVRG"
BASE COUNT          285 a          523 c          593 g          291 t
ORIGIN      
        1 gggcatcgtg ttcaacggga gcaagatcgg gttctccagc ctgagcatga ggagggcccg
       61 agggggggaa gggctcttcg agatccggtc cttggcggcg ttcgccttcc gtttcctcat
      121 ggtggacaag aagttctccc tcaaggcgta cgaccttgtc gatgaaaacc tggccctggt
      181 cgagttttcg tacgattacg acctcgacgg aaacgtcatg cacctcgagg gtcgtctcca
      241 agacggctcc ctcctgaccc gcgtggtcac ccatggcaag gtggaggaaa aggtcttcgc
      301 cgcatgcggc cccgtgtacc cgacgagcgt gctgtacctc tacccggcat tcagggggct
      361 tgcgaccggc aaggtgtacg agatgaccgt gtacgacggc gagaccagga gcgtgtctcc
      421 tgccaggctc gaggtgctcg cctgcgagga gagcgatctc ttcgagggcg ccgggttcaa
      481 ggtcagggtg cagctccact cgcaggaggc gaccgcctgg atcgaccgcc tcggacgccc
      541 cctcctcgag acggccatgg gcggtgtgct cgtctcgggc ctcgaggacg accggcgggc
      601 acgcgagtac cttgcgcgca cctcgctgaa caagggggac gccctgctcg agatgagcct
      661 ggtgccgacc gtgacgaaga tcgaccggcc cagggaggcc gagacgatgg aggtcgtgct
      721 cgtgggcgtg gattccgccc tctccatccc ctcggactgc cgccaggtgt gcacccggca
      781 ggcagacggc gtgcactgca ggatcgagag ggacgctgca ccctgcccct gcggcggcaa
      841 ggacgaccgg gaagggggag gctccctgag gccgagcctc gaggtccaaa gcggcgacgc
      901 gaggatatcg gccctggccg agtccatctc tgcaggcagg aagggcgccc gggaggtcgc
      961 ctccgccgtc gtcgcgtgga tgggtgcgaa cattcgcaag gaggccatcg acgtcttttc
     1021 ggccctggac gtgctcgagc ggaagaaggc cgagtgccag ggccatgccc ttctctacgc
     1081 ggcgctcgcc cgggcgtcag ggatccccac gaaggtggtg agcggcctcg tctattcggc
     1141 cgagcacggc gggttcctct accacgcctg ggccgagagc tgtctcgacg gcgcctgggt
     1201 ggctgtggac ccgaccttcg gccagctgcc cgcggacgcg acacacgtga agctcgtcga
     1261 gggggagtcg cctggcgatc tcatcgccct ggcggggttc gtgggaagga tcagtgccgg
     1321 ggtcctgtcc tgcacgtatg gggggcctga gccttgaccc ggtttcccga catggtgcaa
     1381 gaatcgccgc gtatgcccct gttcccgaaa gtgaaggatg gcttgctgaa gaaggttctc
     1441 cccgcactcg cggcacggct catcaagacc tggtgcctga cctgccgtgt cagggccagg
     1501 atcaacgagt ccgcagagag aaacgccgtg gccgagttcg gcggggcggt gtacgccacg
     1561 tggcaccaga ggatgttcta cttcttccat gacttcggtt ccaggcacgt caccatgatg
     1621 ataagcagga gcaaggacgg cgatatagcc aacgaggtgg cccttcggct cgggtttctc
     1681 tcggtgaggg gg
//
LOCUS       DAOSCR010000602         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47826_length_1690_cov_3.916208, whole genome shotgun sequence.
ACCESSION   DAOSCR010000602 DAOSCR010000000
VERSION     DAOSCR010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47826_length_1690_cov_3.916208"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..>1690
                     /locus_tag="PLT69_11960"
     CDS             262..>1690
                     /locus_tag="PLT69_11960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ15201.1"
                     /translation="MRELRRVTDPTISVIVISWRLHPDNLRSFQILQEQRSENFELIF
                     VDNGGQPGEFTCLEPFIDTLVRLNENTGAYLARNIGAAFSRAPILLFLEDDGIPEKNL
                     IRAHLELFDKYDIIAGRGLYVPKTDNPLNNQQGHYYVGDTEYPTFAHVEGNTTYRADV
                     FFQAGGWDDEIRFGGGGLELSFRLLSIDPDKRKQIYSPAPVIYHDYVTDEAHLENKLA
                     KQALSFERLRRKYPLWDETMKNYRLNIGRDDLLIRRGDATAGQDVPAISLVVTVGPDH
                     RQAMRTLASLGCQDVSGVELVLAYPGEGDTRDAVKEWIAANPAFAVRTFEHAPGEDAS
                     VLDQAVGQARGRYILWISPGVELVDPEAIRILKSTIDDHFDADILYSHKLAVHDPAKE
                     PGMVLFKDWYARKDDLADAMAVGLCIPTDMLLVRKDLYRLVGGYKTLPPGRCQETFVA
                     RVAGMKRYNLKLIDEVLYRSPHDEPY"
BASE COUNT          387 a          546 c          478 g          279 t
ORIGIN      
        1 catgccgaga agttcaaatt ggacccgcaa cagggtgtat cggagggcca tggaggaaca
       61 tgcacttccc ctcatgtcct cggtggaacc gacacgttcg caaggaaccc acagactgac
      121 aaacccttcg acgtctctcc atccgacctc gacgccaccg acgacaatgc ccgcaggcga
      181 caggggcaca ggctcagaga cgctctggat cggaagggct ggtcacacaa atatgacatc
      241 tatgcacggt atttcgaaca cgtgagagaa ctgaggcgcg tcacggatcc cacgatctcc
      301 gtcatcgtca tctcctggcg gctgcacccg gacaacctca ggagcttcca gatccttcag
      361 gagcaacgct cggagaactt cgagctgatc ttcgtggaca acggcggaca gccgggggaa
      421 ttcacctgtc tcgaaccctt cattgacacc ttggtgcgcc tgaacgagaa caccggtgca
      481 tacctggccc gcaacatcgg ggcagcgttt tccagggcac cgatcctcct cttcctcgag
      541 gatgacggga tacccgagaa gaatctcatc agggcccacc tggaattgtt cgacaagtac
      601 gacatcatcg ccggcagggg actctacgtg cccaagaccg acaaccccct gaacaaccag
      661 cagggccact actacgtggg agatacggaa taccccacct ttgcccacgt ggaggggaac
      721 accacctacc gcgccgacgt cttcttccag gccgggggct gggacgacga gatccggttc
      781 ggggggggcg ggctcgagct ctctttccgc ctgctgagca tcgacccgga caagagaaag
      841 cagatctact cccctgcccc ggtcatttac cacgactatg tcacagacga ggcccatctc
      901 gagaacaagc tcgccaagca ggccctatcc ttcgaacggc tcaggaggaa ataccccctc
      961 tgggacgaga ccatgaaaaa ctaccggctc aacatcggca gggacgacct cctcatcagg
     1021 aggggagacg caacggccgg ccaagatgta cccgccattt ccctggtcgt caccgtcggc
     1081 cccgaccata ggcaggccat gagaactctg gcaagcctgg gctgccagga tgtctccggc
     1141 gtcgaattgg tccttgcata cccgggcgag ggggacacga gggatgcggt gaaggaatgg
     1201 atcgctgcca accccgcatt cgccgtccgc acattcgagc atgcgcccgg ggaggacgcc
     1261 tccgtgctgg accaggccgt cggacaagcc cggggcaggt atatcctgtg gatcagtcca
     1321 ggcgtcgaac tcgtcgaccc cgaggccata agaatcctca agagcaccat cgatgaccac
     1381 ttcgatgcgg acatcctcta cagccacaag cttgccgtgc atgaccctgc gaaagaaccc
     1441 gggatggtcc tgttcaagga ctggtacgcc aggaaagacg acctggcgga tgccatggcg
     1501 gtcggcctgt gcatccccac ggacatgctc ctcgtcagga aagacctgta caggctcgtg
     1561 ggcggctaca agacgctccc accgggacgc tgccaggaaa cctttgtcgc cagggtcgcc
     1621 ggcatgaaga ggtacaacct caaactcatc gacgaggtgc tctaccgttc gccgcatgac
     1681 gagccctaca
//
LOCUS       DAOSCR010000603         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47873_length_1689_cov_3.091187, whole genome shotgun sequence.
ACCESSION   DAOSCR010000603 DAOSCR010000000
VERSION     DAOSCR010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47873_length_1689_cov_3.091187"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLT69_11965"
     CDS             complement(<1..538)
                     /locus_tag="PLT69_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOJ15202.1"
                     /translation="MKRFVLGICISVCMVLAIGAQTLHAEEMYKIEVLQVANVRPFKL
                     CFDGFMQELAKNGLVQGKNVTVRRTVIDFDIEKGGVWSKVGVLLRIKSEAQRIAGVKP
                     DLVLTIGTPATKYAKDTIIQAGIPLVFASVAFPQSAGCKSLTEAGKGFTGATLYMSVN
                     DALKIVKLAFPKANVMGII"
     gene            complement(791..>1689)
                     /locus_tag="PLT69_11970"
     CDS             complement(791..>1689)
                     /locus_tag="PLT69_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOJ15203.1"
                     /translation="YRRSLQGFLGELASSGFVAGRNLEVRRKVIGFDMERANLWSKIA
                     VLMQIKSEAMRIADEKPDLVLTIGTPSTKFAKDKIIQAGIPLVFTAVAIPTAAGCTSL
                     THAGQGFTGATLYMDMKEALRLVKQAFPGLTTIGMVHSDDENGVAHVEEAKKTAPTMG
                     MRVLSRQVDKNSPLTPVLEELRAEGVEAFAVPLDTYYGMRDYRACRELESFSLARKIP
                     VFSFAMMKVPGAILYVGSDFQEVGALSGQLAAKIIRKEADPDTLPVARQEDLRVLVDP
                     NMLEKLGWKLPQEILQIAQSVY"
BASE COUNT          274 a          573 c          492 g          350 t
ORIGIN      
        1 agatgatccc catgacgttc gccttgggga aggcgagctt gacgatcttc agggcgtcgt
       61 tcacgctcat gtagagggtc gcccccgtga accccttgcc tgcctcggtc agagacttgc
      121 acccggcgga ctgggggaag gccaccgatg cgaagaccaa cgggatcccc gcctggatga
      181 tcgtgtcctt cgcgtacttg gtcgcgggcg tgcctatggt gagcacgagg tcgggcttga
      241 ccccggctat cctctgggcc tcgctcttga tgcggagcag gacccccacc ttcgaccaca
      301 cgcccccctt ctcgatgtcg aaatcgatga ccgtcctcct gaccgtgacg ttctttccct
      361 gcacgagacc gttcttggcg agttcctgca tgaagccgtc gaaacagagc ttgaaaggtc
      421 gaacgttggc aacctggagt acctctatct tgtacatctc ctccgcgtgc agggtctgtg
      481 cgccaatcgc caggaccatg cagaccgata tgcatatacc caggacaaac ctcttcatga
      541 acatccctcc ttcatgcccc tcggtttttt ccctcaagtt ccctcgtgtc ctaccgccgc
      601 tatccggtga tgcattcgtc catggctgct ccatcagcgg gtcatggacg gtcctctccg
      661 gcacctcggt cgccgcccac aggctccttc ccgtatacgc gcaaggcaag ccgcgacctc
      721 gacgcccgag aaccgcggcc tcggattccc tgcatggcag gaccaggggc caagccctgg
      781 cccgcctctt ttagtacacg ctctgcgcga tctgcaggat ctcctgcggc agtttccacc
      841 ccagcttctc gagcatgttg gggtccacca ggaccctgag atcctcctgc ctcgccacgg
      901 ggagcgtgtc cgggtcagcc tccttcctga tgatcttcgc ggcgagctgg cccgagagcg
      961 cccctacctc ctggaagtcc gacccgacgt agaggatcgc gccaggcacc ttcatcatcg
     1021 cgaagctgaa cacggggatc ttgcgtgcga gcgagaagct ctccagttcc cggcaggccc
     1081 tgtagtctcg catgccgtag tacgtgtcga gggggacagc gaacgcctcg acgccctcag
     1141 ccctgagctc ctcgaggacc ggggtcaggg ggctgttctt gtccacctgc ctgctcaaga
     1201 ccctcatgcc catcgtcggc gcagtcttct tggcctcttc cacgtgcgca acgccgttct
     1261 cgtcgtcgct gtggaccatg ccgatggtcg tgagcccggg gaaggcttgt ttgacgagcc
     1321 tgagggcttc cttcatgtcc atgtagaggg tggcgccggt gaacccctga ccggcatgcg
     1381 tgagcgacgt gcagcctgcc gcagtcggga tcgccacggc cgtgaacacc agggggatgc
     1441 ccgcctggat gatcttgtcc ttggcgaact tggtggacgg cgtccctatg gtgaggacga
     1501 ggtcgggctt ctcgtccgct atgcgcatgg cctcggactt tatctgcatg agcacggcga
     1561 tcttgctcca gaggttcgcc ctctccatgt cgaacccgat gaccttgcgc ctcacctcga
     1621 ggttcctgcc ggccacgaag ccgctcgacg cgagctcccc gaggaacccc tggagggatc
     1681 tgcggtacg
//
LOCUS       DAOSCR010000604         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_47970_length_1687_cov_3.014706, whole genome shotgun sequence.
ACCESSION   DAOSCR010000604 DAOSCR010000000
VERSION     DAOSCR010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47970_length_1687_cov_3.014706"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1433)
                     /locus_tag="PLT69_11975"
     CDS             complement(51..1433)
                     /locus_tag="PLT69_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ15204.1"
                     /translation="MGEFDELIEEFVTESKEHIADIEDDLIRIESDMENVDPEVINRV
                     FRAAHSIKGSSKFLDLVNIGELAHRMEDVLNLIRSGKLSPTSEVASVLLLSADTLKTM
                     LDDVNKSNDMDISEHLRELQKILDSPETVKGGAKSACAYTNGPSPLAAFDVEPSMIRG
                     KLASSQVYVITLTPGSCDIEHVLSELGNLGEVIAESAANDGSLSVLFSTIMEPDMLPL
                     ALGVPRNAYVQIDESMLKAVRAQPKAAPDEKAKPRPKATPRPMQAARPEAPAANQTRE
                     PVLTGECSEISYDDADTQGALASLNEDIRAARASENTSYITFTLDNENYAVPIQSIEE
                     IIGLQEISLLPNVPDFIKGVINLRGEIVPIMDLRIKFGLPPKEYNQFTVFLIMRVDER
                     LMGLVVDNVADVLVIDPSKVQKRPSFSAKISTAFINGVYKDSQDELVILMDVPALIRP
                     EEWEADRPRA"
BASE COUNT          270 a          512 c          530 g          375 t
ORIGIN      
        1 cacctggata acgctcggga tggccggcct gctggcgagg acctttctct tcaggcccgg
       61 ggccggtccg cctcccactc ctcgggcctg atgagcgcgg ggacgtccat gaggatcacg
      121 agctcgtcct ggctgtcctt gtagacgccg ttgatgaacg ccgttgatat cttggcggag
      181 aacgagggcc tcttctgcac cttcgaagga tcgatcacga ggacgtccgc cacgttgtcc
      241 accacgagcc ccatgagcct ttcgtcgacg cgcatgatga ggaacacggt gaactggttg
      301 tactccttgg gtgggagacc gaacttgatc ctgaggtcca tgatgggcac gatctctccc
      361 ctgaggttga tcaccccctt gatgaagtca ggcacgttcg gcaggaggct gatctcctgg
      421 aggccgatga tctcctcgat gctctggatg ggcaccgcgt agttctcgtt gtcgagggtg
      481 aaggtgatgt aagaggtgtt ctccgaggcc ctggcggcgc ggatgtcctc gttgagggat
      541 gcgagcgccc cctgtgtgtc cgcgtcgtcg taggagatct cgctgcactc ccctgtcagg
      601 acgggttccc tcgtctggtt ggccgcaggg gcttcaggcc ttgccgcctg catgggcctc
      661 ggtgtggcct ttggcctggg tttcgccttc tcgtccgggg cggctttggg ctgagcccgt
      721 acggctttga gcatggattc gtcgatctgg acgtacgcgt tcctgggaac cccgagtgcg
      781 agcgggagca tgtccggttc catgatcgtg gagaacaaga cggagagcga accgtcgttt
      841 gcggcgcttt ccgcgatgac ttcaccgagg ttgccgagct cggagaggac gtgctcgatg
      901 tcgcatgaac cgggtgtgag cgtgatgacg tagacctgtg atgaggccag tttgcctcgt
      961 atcatcgatg gctccacgtc gaacgcggcc aggggcgagg gaccgttcgt gtacgcgcac
     1021 gcagacttgg ccccgccctt gaccgtctcg ggcgagtcca ggatcttctg gagctccctg
     1081 aggtgctcgg agatgtccat gtcgttgctc ttgttgacgt cgtcgagcat ggtcttgagc
     1141 gtgtccgcgg acaaaaggag cacgcttgcc acctcgctcg tgggcgagag tttgcccgag
     1201 cggatgaggt tcaggacgtc ttccatgcgg tgggccaact cgccgatatt gacgaggtcg
     1261 aggaacttcg agctcccctt gatggagtgc gccgccctga agacgcggtt gatgacctca
     1321 ggatccacgt tctccatgtc cgactcgatc ctgatgaggt cgtcctcgat atccgctatg
     1381 tgctctttcg actcggtcac gaattcctcg atgagctcgt cgaattcgcc catggtgcac
     1441 tccctcctgt gggtaggtct tggtatacca tcggcagata ccggcctacg acttaacccc
     1501 ctctcgggtc gacccaccac gctctccctg cagcagcctg caagcgtgcc cacggcatcc
     1561 acacagcccc tggatcatcc ccctgcgggt gcccatgggc catggcgtct tcaccgaggg
     1621 tgcatccttc atgttcttca cgcggccgtg cgccaagagc gaaaacaaaa ggcgcctcct
     1681 ccttccc
//
LOCUS       DAOSCR010000605         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48017_length_1686_cov_2.294911, whole genome shotgun sequence.
ACCESSION   DAOSCR010000605 DAOSCR010000000
VERSION     DAOSCR010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48017_length_1686_cov_2.294911"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PLT69_11980"
     CDS             <1..1545
                     /locus_tag="PLT69_11980"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ15205.1"
                     /translation="GDRLCIPVLLYHCFGMVMGNLGCLTHGATMVYPGEAFEPLAALI
                     DDEEWIRFSVIEAISHLNHERLPSFIKEQMGRFRNDDLTMCALLEASGASGSRELAHF
                     LVELLEEASMTIECEVVGAILRLLSPEEIAGLHADKARAVKEVIERHVGSVDGDLQLS
                     MFRALEAVGDGRSVQVLLEDARSLDPDARPEVFDAIEETMAAIGDVEAVSSLLEGEEK
                     IVVLGSRVLGRIGGSRASKIVAQCIPASQGPAKRALVDALVEIDVHGNRETFKALLSD
                     RDGHVLRSSITALGLCGGQEDIASLEPFLSHPYPDVREAALGAIVEIDTDQAQQVFTR
                     MMGKDDPESRRMALDGLSRLSSPLIGKYVVECLRDPSNEVRLMAATIVRDREIPLDTE
                     AMRDLLRDPSDAVRCVAIDMVGQRKVEGLRPVLEEWMEGDDMRIASHALDALGAFGDD
                     RAVESLVRVVRKGSDFLRISAVRVLGGLGREDIVGEIEAFVDDPNPDVARAVMDAMDR
                     LRGEGF"
BASE COUNT          303 a          510 c          605 g          268 t
ORIGIN      
        1 ggcgaccggc tgtgcatccc ggtcctgctc tatcactgct tcggcatggt gatgggtaac
       61 ctcggctgcc tgacccacgg cgccaccatg gtctatccgg gcgaggcctt cgagcccctg
      121 gctgctctca tcgacgacga ggaatggatc cgtttcagcg tgatcgaggc gatatcgcac
      181 ctcaaccacg agagactgcc ctccttcatc aaggagcaga tgggcaggtt caggaacgac
      241 gacctcacca tgtgcgccct gctggaggcc tccggcgcga gcgggtcccg ggagttggcg
      301 cactttctgg tcgaactgct cgaagaggcg tcgatgacca tcgagtgcga ggtggtgggc
      361 gcgatcctga ggctcctcag cccggaggag atagccgggc ttcatgctga caaggcaagg
      421 gcggtcaagg aggtcatcga gcggcacgtg gggtccgtgg acggagacct ccagctctcc
      481 atgttcaggg cgctcgaggc cgtgggcgac gggcggagcg tccaggtgct cctggaggac
      541 gcccgctcgt tagaccccga cgccaggccg gaagtcttcg atgccatcga ggagaccatg
      601 gccgcgatcg gggacgtgga ggcggtctcg agcctcctcg aaggcgagga gaagatcgtc
      661 gtgctcggat ccagggtgct gggacgcatc ggcggatcga gggcgtcgaa gatcgtggcg
      721 caatgcatcc ccgccagcca ggggccggcc aagagggcgc tcgtggacgc gctcgtggag
      781 atagacgtcc acggaaaccg ggagacattc aaggccctcc tttctgaccg ggacgggcac
      841 gtcctcaggt ccagcatcac ggcgctcggc ctgtgcggcg ggcaggagga catcgcctcc
      901 ctggagccct tcctctccca tccctacccg gacgtgaggg aggcggctct cggggcaatc
      961 gtggagatcg acacggacca ggcccagcag gtcttcaccc ggatgatggg gaaggacgat
     1021 cccgagtcgc gcaggatggc ccttgacggc ctgtcgaggt tgtcgagccc gcttatcggg
     1081 aaatacgtcg tcgaatgcct gagagacccg agcaacgagg tccgtctcat ggccgccacc
     1141 atcgtccgcg accgggagat acccctcgac accgaggcga tgagggacct gctccgggac
     1201 ccgagcgacg cggtcaggtg cgtcgccatc gacatggtgg gacagagaaa ggtggaggga
     1261 ctcaggccgg tcctggagga atggatggaa ggcgacgaca tgaggatcgc gtcccacgcg
     1321 ctcgatgcgc tcggcgcctt cggggacgac cgtgcggtgg agtccctggt ccgggtcgtg
     1381 agaaagggct cggacttcct gaggatatcg gccgtcaggg tgctgggggg cctcggcagg
     1441 gaggatatcg taggggagat cgaggcgttc gtggacgacc ccaacccgga tgtcgcgagg
     1501 gccgtcatgg atgcgatgga caggctcagg ggggagggct tctgacatgg aaccgccggt
     1561 gctcgaaagg ctcaaggcga tcgatgacct gcccacgctc ccctcggtgg cgctcgaggt
     1621 gctcagcctc gcgcacaggc cggacgtgac gatccagctc gtggccgaat gcatccacag
     1681 ggaccc
//
LOCUS       DAOSCR010000606         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48041_length_1685_cov_3.628221, whole genome shotgun sequence.
ACCESSION   DAOSCR010000606 DAOSCR010000000
VERSION     DAOSCR010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48041_length_1685_cov_3.628221"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLT69_11985"
     CDS             complement(<1..783)
                     /locus_tag="PLT69_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOJ15206.1"
                     /translation="MAMRWKLRERLTALVLCTGLIPLIVVIVYTGIRISGHLKHQASD
                     YLKVKVDAFARMAEVRSASIEGNLDIIKEQLMKSLKNELINEASKERYFETGYLTIFT
                     SDGTCVYHPKAEFRNNKTLYNENDFVKKALSKKQGSFLYTFGGQDKISYLAYIKPLDW
                     VIWAAVPASEALSEVRMLQVELFAFLIVTAIVVAVLGILFSSRIASRARQISNSMMDI
                     AQGEADLSKRLPVLSTDEIGEIASWFNTFVENLEEVIGKVKQA"
     gene            complement(848..>1685)
                     /locus_tag="PLT69_11990"
     CDS             complement(848..>1685)
                     /locus_tag="PLT69_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ15207.1"
                     /translation="LKHMIRNSIDHGIESPEGRRRKGKPETGTIWLKAYQREGNIYIE
                     ISDDGRGIDKAKVLAKAVEKGIAEPGRSYTDKEIYEMLFAPGLSTADRVSELSGRGVG
                     MDVVKRNIEELRGTVEISSEENKGSTFKVNLPLTLAIIDGMMIRVGSEILTIPLAMID
                     KSVRPSRTEIKTVEGKGELVDIRGDYLPLVRLYDLFRIPSEKTDPTEALVVVLHGAEN
                     RFGILVDDVLGQTQAVIKSIDKNFKKIEGTSGATILGNGRVSLILDVHGIEKMAFVGS
                     AM"
BASE COUNT          280 a          525 c          477 g          403 t
ORIGIN      
        1 cgcctgcttc accttgccga tcacctcctc gaggttctcg acgaacgtgt tgaaccagga
       61 ggcgatctcc ccgatctcgt cggtggacag gaccgggagc cgtttggaga ggtccgcctc
      121 gccctgggct atgtccatca tcgagtttga gatctggcgt gccctcgagg ctatcctgct
      181 cgagaagagg attcccagga ccgccaccac gatcgctgtc acaatgagga aggcgaagag
      241 ctcgacctgg agcatcctca cctcgctcag ggcctcgctc gcaggcaccg ctgcccagat
      301 cacccagtcg aggggcttga tgtatgccag gtagctgatc ttgtcctgtc cgccgaaggt
      361 gtagaggaaa gaaccctgtt tcttgctcaa ggccttcttc acgaagtcgt tctcgttgta
      421 cagggtcttg ttgttgcgga actcggcctt cgggtggtag acgcacgtgc cgtccgaggt
      481 gaagatggtg aggtagcccg tctcgaagta cctttccttc gacgcctcgt ttatgagctc
      541 attcttgagg gatttcatga gctgttcctt gatgatgtcg aggttgccct cgatcgaggc
      601 gctcctgacc tcggccatcc tcgcgaacgc atccaccttg accttcaggt agtcagaggc
      661 ctgatgcttg aggtgaccgc tgatcctgat gccggtgtag acgatgacca cgatgagcgg
      721 tatgagcccc gtacagagta cgagcgccgt gagcctttct ctgagtttcc acctcatcgc
      781 catgacgtta tctcctttta cgaattgtgg tatatgccag ggtgtttgta tgccgcctcg
      841 aaccctctca catggccgac cccacgaagg ccatcttctc gatgccgtgg acgtccagga
      901 tcagggacac cctcccgttc ccgaggatgg tggccccgga agtgccctcg atcttcttga
      961 agttcttgtc gatgctcttg atcaccgcct gtgtctggcc gagcacgtcg tccacgagga
     1021 tgccgaagcg gttctctgcg ccgtgaagca ccacgacgag cgcctctgtg ggatccgtct
     1081 tctcggacgg gatcctgaag agatcgtaga ggcgcaccag cggcaggtag tcacccctga
     1141 tgtccacgag ctcgcccttg ccctcgacgg tctttatctc cgtccttgac ggcctgaccg
     1201 acttgtctat catggccagg ggaatggtga ggatctcgct gccgaccctg atcatcatgc
     1261 cgtcgatgat ggccagcgtg agcggcaggt tgaccttgaa ggtggagccc ttgttctcct
     1321 cggaagagat ctcgacggtt cccctcagct cctcgatgtt cctcttcacc acgtccatcc
     1381 cgacccccct gccggaaagc tcgctcaccc tgtctgcggt ggagagcccc ggagcgaaga
     1441 gcatctcgta gatctccttg tcggtatacg acctgcccgg ctccgcgatg cccttctcta
     1501 cggcctttgc gagcaccttc gccttgtcga tgcccctgcc gtcgtcggat atctctatgt
     1561 agatgttccc ctcgcgctgg taggccttga gccagatggt gcccgtctcg ggcttgccct
     1621 tccttctcct tccctcggga gactcgatgc cgtggtcgat cgagttgcgg atcatgtgct
     1681 tgagg
//
LOCUS       DAOSCR010000607         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48149_length_1683_cov_3.123464, whole genome shotgun sequence.
ACCESSION   DAOSCR010000607 DAOSCR010000000
VERSION     DAOSCR010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48149_length_1683_cov_3.123464"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..960)
                     /gene="pyk"
                     /locus_tag="PLT69_11995"
                     /pseudo
     CDS             complement(37..960)
                     /gene="pyk"
                     /locus_tag="PLT69_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            complement(957..>1683)
                     /locus_tag="PLT69_12000"
     CDS             complement(957..>1683)
                     /locus_tag="PLT69_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HOJ15208.1"
                     /translation="NGALNLSVQDGWWVEGYRPDLGWSIGNGEVYDNLEYQDHVESKA
                     IYDLREREIIPLFYDRGLDGLPRGWISMMKKSMRTLCPVFNSNRMVEEYTDRCYVTAS
                     LTLKPLVAEGYKNARQLVKWKDTIASRWAEVAVLDMSHEGGDEVSVHSEMTIRAEVKL
                     GAISPSDVSVRAYYGTVDDAGNLSAAKDVLMDHMKEMGKGVHLFETRITCDTTGRFGY
                     SVRVLPSHPSLIHPVDMGLICWA"
BASE COUNT          256 a          597 c          527 g          303 t
ORIGIN      
        1 gcggcccatg gccgactccc cggagagcat cactcagtcg gtcccgtcga gcaccgcgtt
       61 cgcgacatcg gtggcctctg ccctggtggg gcgggggctc gaggtcatgg actcgagcat
      121 ctgggtcgcc gtgatcaccg gcttgcccag caccaccgcc tggtggatga tctgcttctg
      181 caccacggcc atgcgctcga tgggcacctc caccccgaga tcgcccctgg cgaccatgac
      241 cccgtcggag gccctcacga taccctcgag gtcatcgagc gcgcaggcgc gctcgatctt
      301 cgccaccacg aacgggtggt accccatccc ggcggcctcg ccgcgcagtc tcgtcacgtc
      361 ctctgcccgc gagacgaacg acatgcacac ggcgtccacg cccttggcgg cgcagaagcc
      421 aaggcacgtg cggtcagtgg gcgtgagcgg gtctatcccg agcgagacgc ctggcaggtt
      481 gagccccttt cgcgacctga gctcgccccc gacgaccacc gtgcagcgca cctcggcgcc
      541 ctccacgcgg tccaccgcga gctggacgag cccgtcgttg atgaacagcg tgtccccggg
      601 cttgacgacc ctgggcagtt cagggaagct gacagacacc ctgcggccgt ccccaacgat
      661 atcttccgtg gtgagcgtga accgggcgcc cgccacgagc tccacgggct cgtcggcgat
      721 acggccgatg cgcatcttgg gccccggcag gtcggccatg acggtgatcc tccgtgccgt
      781 cctcctctcc gcctccctca cggcctcgat gaccctggca tggcggtcga gatcgccgtg
      841 ggaaaagttg atcctcgcca cgctcatgcc cttttcggcg agccgctcga ggatcgcggg
      901 ctcgctggaa gcgggcccga tggtgcagac gatcttggtc ctgcaggagg ggaccctcat
      961 gcccagcata tgagccccat gtccaccggg tggatgaggc tcgggtgcga gggcaggacc
     1021 cgcaccgagt agccgaacct gcccgtggtg tcgcaggtga tgcgcgtctc gaagaggtgc
     1081 acgcccttgc ccatttcttt catgtggtcc atcagcacgt ccttggcggc cgagaggttc
     1141 ccggcgtcgt cgaccgtccc gtagtaggcc ctgacgctca catccgaggg gctgatggcg
     1201 ccgagcttca cctcggcccg tatggtcatc tcgctgtgga cggacacctc gtcgcccccc
     1261 tcgtggctca tgtcgaggac cgcgacctcc gcccacctcg aggcgatcgt gtccttccac
     1321 ttcacgagct ggcgggcatt cttgtaccct tccgcgacca gcggcttcag ggtgagggat
     1381 gccgtcacgt agcagcggtc ggtgtactcc tcaaccatgc ggttggagtt gaacacgggg
     1441 cacagcgtgc gcatggattt cttcatcatg gatatccagc ccctgggaag gccgtccagg
     1501 cccctgtcgt agaacagggg gatgatctcg cgctcccgca ggtcgtagat cgccttgctc
     1561 tccacgtgat cctggtactc gaggttgtcg tacacctcgc cgttgcctat ggaccagccg
     1621 agatccggcc tgtatccctc gacccaccag ccgtcctgca ccgagaggtt cagcgcgccg
     1681 ttg
//
LOCUS       DAOSCR010000608         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48271_length_1680_cov_3.088615, whole genome shotgun sequence.
ACCESSION   DAOSCR010000608 DAOSCR010000000
VERSION     DAOSCR010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48271_length_1680_cov_3.088615"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..811
                     /locus_tag="PLT69_12005"
     CDS             41..811
                     /locus_tag="PLT69_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ15209.1"
                     /translation="MLSVKNIETWYDLIRALHGISFEIKEGAIVALLGSNGAGKSTTL
                     KTIMRLLDDDQPEKGTIEFMGRRIDRLDTEEIVRMGISYVPEGREVFPELTVMENILM
                     GAYTRRDRKGVQEDIETVFGHFPILKQRAGQQAGHLSGGEQQMLAIGRALMSRPKLLM
                     LDEPSLGLSPLLTREIFSIIRDINERGVTILLVEQNVNMALKYSTFAYLMENGRIVRA
                     DKPEVLREDEDVKEFYLGVATEQSVKGYKRYRRKVRFR"
     gene            811..>1680
                     /locus_tag="PLT69_12010"
     CDS             811..>1680
                     /locus_tag="PLT69_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15210.1"
                     /translation="MRHGKTPDIGILDDPSVKTLPQALRAIVRRQPDRVAMRMKEYGI
                     WHDITWRQYYENVKKTAMGLYALGVRRGDKVAIVGENKPEWLYSALGVMSVGATFVGV
                     YTTNPAPECEYVVGHSESVVFFCEDEEQFDKALTFKDRTPSLRKAVVWDMEGLRHFDD
                     PILMSFDDLLALGEATNAENPDLFDLLVDDGEAGDIASIIYTSGTTGPPKGAMIAHEN
                     YLWIARQTRKITRMSAEDETISFLPLNHVYEQIFDLMMHLRVGHVVNFTENTDTVMND
                     LKDVSPTLFHAVPR"
BASE COUNT          370 a          511 c          537 g          262 t
ORIGIN      
        1 accccgaagt catagccgca tacctgggcg aggaggattg atgctctcgg tcaagaacat
       61 cgagacgtgg tacgacctca tcagggccct gcacggcatc tcgttcgaga tcaaggaggg
      121 ggccatcgtg gccctgctcg ggagcaacgg agcgggcaag tccacgacgc tcaagaccat
      181 catgcgtctc ctcgacgacg accagcccga aaaggggacc atcgagttca tgggcaggcg
      241 catcgacagg ctggataccg aagagatcgt gcgcatgggt atcagctacg tgcccgaggg
      301 acgcgaggtg ttcccggagc tgacggtcat ggagaacatc ctcatgggtg cgtacacgcg
      361 aagggacaga aagggcgtcc aggaggacat cgagaccgtg ttcggccact tccccatcct
      421 caagcaacgc gcagggcagc aggcgggcca cctgagcggc ggcgagcagc agatgctggc
      481 catcggcagg gcgctcatga gcaggccgaa gctcctcatg ctcgacgaac cgtctctcgg
      541 cctctccccg ctcttgacca gggagatctt ctccatcatc agggacatca acgagcgggg
      601 ggtgaccatc ctgctcgtgg agcagaacgt gaacatggcg ctcaagtact ccaccttcgc
      661 atacctcatg gaaaacggcc gcatcgtcag ggcggacaag ccggaggtgc tcagggagga
      721 cgaggacgtc aaggagttct acctcggggt tgccacggag cagtcggtca agggctacaa
      781 gagatacagg agaaaggtga ggttcaggtg atgcggcacg ggaaaactcc ggatatcggc
      841 atcctcgacg acccgtctgt gaagacactg ccccaggcgc tcagggcgat cgtcaggagg
      901 caacctgatc gcgtggccat gcgcatgaag gagtacggca tatggcacga catcacctgg
      961 cggcaatact acgagaacgt gaagaagacc gccatgggcc tgtacgcgct cggggtcaga
     1021 cggggagaca aggtcgccat cgtgggggag aacaagccgg agtggctcta cagcgcgctc
     1081 ggcgtcatgt ccgtgggtgc gaccttcgtg ggggtgtata ccaccaaccc cgcccccgag
     1141 tgcgagtacg tggtgggaca ctccgagagc gtggtcttct tctgcgagga cgaggaacag
     1201 ttcgacaagg ccctgacgtt caaggacagg acgccgagtc tcaggaaggc ggtcgtctgg
     1261 gacatggagg ggctccgcca cttcgacgac cccattctca tgagcttcga cgacctgctg
     1321 gccctcggtg aggccacgaa cgccgagaac cccgacctct tcgatctcct cgtggacgac
     1381 ggcgaggccg gcgacatcgc gtccatcatc tacacctcgg ggaccacggg cccgcccaag
     1441 ggggccatga tcgcccacga gaactacctc tggatcgcga ggcagacgag gaagatcacc
     1501 cgcatgtcgg cagaggacga gaccatatcg ttcctcccgc tgaaccacgt ctacgagcag
     1561 atcttcgacc tcatgatgca cctgcgggtc ggccacgtgg tgaactttac cgagaacacg
     1621 gacacggtca tgaacgacct caaggacgtc tcccccaccc tcttccacgc ggtgccgcgc
//
LOCUS       DAOSCR010000609         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48397_length_1677_cov_3.316893, whole genome shotgun sequence.
ACCESSION   DAOSCR010000609 DAOSCR010000000
VERSION     DAOSCR010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48397_length_1677_cov_3.316893"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PLT69_12015"
     CDS             <1..154
                     /locus_tag="PLT69_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOJ15211.1"
                     /translation="VEGLSNEEIAQALGLSVPAVKSRLHRGRLILRDMLSGYFRKYTL
                     EGDHPA"
     gene            151..399
                     /locus_tag="PLT69_12020"
     CDS             151..399
                     /locus_tag="PLT69_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15212.1"
                     /translation="MKCLVCSSLFPDYMENCLSQDVIDELEHHFRFCEKCRVCFSTYR
                     LTVMLSSKVEQPAAVSPESVERLTSLLIQRFFNGARGA"
     gene            735..>1677
                     /locus_tag="PLT69_12025"
     CDS             735..>1677
                     /locus_tag="PLT69_12025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ15213.1"
                     /translation="MSSLHEIQTRFTPGARRRIKDAPLNICLYTYRGKPTSGGQGVYV
                     KRLSRALKDLGHNVEVVSSPPYPNIDKDIPLHKIPSLDLYNPDDLFRVPSLRELRNPL
                     NLLEWLGVSTGGFPEPFTNSIRYFSFMRRNLHRYDVVHDNQCLAYGILGVKFLGIPTV
                     ATIHHPITVDRDVEVASVTPLWRKLKVRRWYSFLSMQGRVSRRISHIITVSKASRKDI
                     SRAFSIPLERFRVVPNGINMDIFHPVDGVQRLENHIMVTNSADTPLKGLRYLIEAVAS
                     LRKERPVHLTVIGSPKKDGEIERLVGSLGAREYITFTG"
BASE COUNT          337 a          552 c          484 g          304 t
ORIGIN      
        1 cgtcgagggc ctgtcgaacg aggagatcgc ccaggcgctc ggcctgagcg tgccggccgt
       61 gaagtcgcgg ctccaccggg gccggctcat cctcagggac atgctctcgg gatacttcag
      121 gaaatatacc ctcgagggag accatcccgc atgaaatgcc tggtctgttc ttctctcttc
      181 ccggactaca tggagaactg cctctcccag gacgtgatcg acgagcttga gcaccacttc
      241 aggttctgcg agaagtgcag ggtctgtttc tccacctaca ggctcacggt catgctctcc
      301 agcaaggtcg agcagcctgc ggcggtgagc cccgagtccg tggaaaggct caccagcttg
      361 ctcatccagc gcttcttcaa cggcgcccgc ggagcatagg gggcacggcc cgacctcgct
      421 caggcgggac ggccccgtct gacgggccga cctgcatgtc gtaccaccgt cccgggttca
      481 tcctgccgca tgaccccagg gcgccctcca tggcgggacg gcctcgatgg agcgcgagcg
      541 aagccgccag agacccaatc cgacggacga ccggggggaa atcgttctct ttcttcacac
      601 cattcgtttc tatgggcaga aatgaatgga ttttccttgc cttgtcagtg aagagttgct
      661 aagataccga ttcgcctgta gacgggaagt ccacgtcgtc gagcacgatc accaaaagag
      721 gatgttattg cagtatgtca tcgttgcatg agatacagac tcgtttcacc cccggggcac
      781 ggaggcggat caaggacgca cccctcaaca tctgtctcta cacctaccgc ggcaagccga
      841 catcaggcgg ccagggggtg tacgtcaagc ggctgagccg ggccctcaag gacctcgggc
      901 acaacgtcga ggtcgtgtct tcacctccgt atccaaacat cgacaaggac atccccctcc
      961 acaagatccc gagcctcgac ctatacaacc cggacgatct cttccgggtg ccgagcctca
     1021 gggagctgag gaaccccctg aacctcctcg agtggctcgg cgtgtccacc gggggcttcc
     1081 ccgagccgtt caccaacagc atccgctact tctccttcat gaggaggaac ctgcaccgct
     1141 acgacgtggt ccacgacaac cagtgcctgg cctacggcat cctgggcgtg aagttcctcg
     1201 gcatcccgac ggtggccacc atccaccacc ccattaccgt ggacagggac gtggaggtgg
     1261 catccgtcac ccccttgtgg cgcaagctca aggtgaggag gtggtactcg tttttgtcca
     1321 tgcagggacg ggtatcccgc aggatctccc acatcatcac ggtgtccaag gcctcccgga
     1381 aggacatcag ccgcgccttc tccatcccct tggagaggtt cagggtggtg cccaacggca
     1441 tcaacatgga catcttccat ccagtggacg gcgtgcaacg gctcgagaac cacatcatgg
     1501 tcaccaacag cgccgacacg ccgctgaagg gcctgcgcta cctcatagag gcggtggcgt
     1561 ctctcaggaa agagcggccc gtgcacctca cggtcatcgg ctcccccaag aaggacgggg
     1621 agatcgagcg actcgtgggg tcgctcggag cccgggaata catcaccttc accggaa
//
LOCUS       DAOSCR010000610         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48481_length_1675_cov_2.054321, whole genome shotgun sequence.
ACCESSION   DAOSCR010000610 DAOSCR010000000
VERSION     DAOSCR010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48481_length_1675_cov_2.054321"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLT69_12030"
     CDS             <1..972
                     /locus_tag="PLT69_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15214.1"
                     /translation="VAALVFSGPGHPEKARKGDPAHGIAAPGILGAPGVSPDAMLEEE
                     YVRELRKYYGPTISKKSTQASLFFLRNQLAGLGKGGAPGLFERILKRAFPANAKDILE
                     TIAKLDLYEKWLVDNRRMLLSLPPEQRLEVLWRKRTELFGPEAREMFSGEMLAVEART
                     ARVRETLDALDEAADTPMDDRLELYEDVLRQTYENTPEAFILQQKELLARVFFSIDSV
                     QDELRRMDPVARQQEINRIRRKMGMSEEEMAARTKRDQDRELRWQIGLRYMEEREALE
                     SRYTGAELEERLKALRQEYFDDEATTIELEEQQGFFRFKRPRIYGRN"
     gene            1231..>1675
                     /locus_tag="PLT69_12035"
     CDS             1231..>1675
                     /locus_tag="PLT69_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ15215.1"
                     /translation="MKKKDDATFGARIRALRLEKGLSLEDLAGETGYPPELLGRVEDG
                     LVAPPVALVLQLGRAFRVDIEQLAGETERRATQRRARSHKKRVASYAYTPLTNPAPDK
                     HLRAYLVSLEAHEDHKGVEYHHEGEEFIYVLEGSLVVRVGRNVTTL"
BASE COUNT          358 a          518 c          582 g          217 t
ORIGIN      
        1 gtcgccgccc tggtcttctc cgggcccggg cacccggaga aggcacgaaa aggcgaccct
       61 gcacacggca ttgccgcccc ggggatcctc ggcgcgcccg gcgtctctcc ggacgccatg
      121 ctcgaagagg agtacgtaag ggagctcagg aagtactacg gccctaccat ctcgaagaag
      181 tccacccagg cgtcgctctt cttcctcagg aaccagctgg caggcctcgg caaaggggga
      241 gcgcccggcc tcttcgagcg gatcctgaaa cgggccttcc cggcgaacgc gaaagacatc
      301 ctcgagacca tcgcgaagct ggacctctac gagaagtggc ttgtggacaa caggcgcatg
      361 ctgctttccc tgcctccgga gcagcggctg gaggtgctct ggcggaagag gaccgaactc
      421 ttcggcccag aggccaggga gatgttctcc ggcgagatgc tcgccgtgga ggcccgcacg
      481 gcgagggtga gagagacctt ggacgccttg gacgaagcag cggacacccc catggacgac
      541 aggctcgagc tctacgagga cgtgctcagg cagacctacg agaacacccc cgaggccttc
      601 atcctccagc agaaggaact cctcgccagg gtgttcttca gcatcgactc ggtgcaggat
      661 gagctcaggc gcatggaccc ggtcgcacgc cagcaggaga tcaacaggat caggcggaag
      721 atgggcatga gcgaggagga gatggcggcc aggacgaaga gggaccagga cagggagctc
      781 aggtggcaga tagggctcag gtacatggag gaacgggagg ccctcgagag ccggtacacg
      841 ggcgccgagc tcgaggaacg tctcaaggcc ctcaggcagg agtatttcga cgacgaggcc
      901 acgaccatag agctcgagga gcaacagggg ttcttcaggt tcaagaggcc ccggatctac
      961 gggaggaact aggggggata gggacgtccc cgcgacccat gagcctgcag agagccgggt
     1021 atgccatgga tcgacggggg gacagagacg gcctcgggca gaggatcccg caggtcatgc
     1081 acggccggcc ggttcccgcc gggaggaagg cctcatcctc tgccgggggg ccttcccgat
     1141 gacatgccgg cctcacgtga gacccagcat cgacaaggga agcctcaggc ttacaccgct
     1201 cgagagagga cggagaaggg agagcaccgc atgaagaaaa aggacgatgc aaccttcggg
     1261 gcgcgcatcc gggccctgag gctcgagaag gggttgagcc tcgaggacct cgcgggtgaa
     1321 accgggtacc cgccggagct cctcggccgg gtcgaagacg ggctcgtggc tccgccggtg
     1381 gcgctcgtcc tgcagctggg cagggcattc agggtggaca tcgagcagct agcaggggag
     1441 acggaacggc gcgccaccca gagacgcgcc aggagccaca agaagcgggt cgcctcctac
     1501 gcctacaccc ccctcaccaa cccggccccc gacaagcacc tgcgcgccta cctggtgagc
     1561 ctggaagctc acgaggacca caagggcgtc gaataccacc acgagggcga ggagttcatc
     1621 tacgtcctgg agggcagcct ggtcgtcagg gtgggcagga acgtcacgac cctcg
//
LOCUS       DAOSCR010000611         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48698_length_1669_cov_3.192069, whole genome shotgun sequence.
ACCESSION   DAOSCR010000611 DAOSCR010000000
VERSION     DAOSCR010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48698_length_1669_cov_3.192069"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..810
                     /locus_tag="PLT69_12040"
     CDS             172..810
                     /locus_tag="PLT69_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOJ15216.1"
                     /translation="MTDLLAITVSSFFLAFSGAAMPGPLMTATIGESARRGPVAGPAL
                     VAGHGILELLLVILLMLGLAPFLKQDMVFAVIAMAGALMLAGMGVSMLLKVPGLTLSR
                     EASGGGMSRLVAAGAVLSLSNPYWTLWWSTIGLTYILYSARLGNAGVALFFAGHLLGD
                     LAWYSALSFAVGRGRRFMSDRSYRVIVALCSLFLVVLAAVFAVSGLRTFLSV"
     gene            1314..>1669
                     /locus_tag="PLT69_12045"
     CDS             1314..>1669
                     /locus_tag="PLT69_12045"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ15217.1"
                     /translation="MRDRAAASVMASFIGDALALGVHWEYSTRKLRQEFGRVDSYLDP
                     GPGSYHRTRKKGEFTHYGDQAYVLLESVASSGAFDAEDFSRRWRRLFEDYDGYLDQAT
                     RTTLENLSRGVPFLEAG"
BASE COUNT          238 a          521 c          606 g          304 t
ORIGIN      
        1 ggaccgcgtg ccgcctgtgg cccggtacaa agacgcaggc gggcgggtcg tggaggcact
       61 ctctttgcct cttgtgaaca gccgcatcga tatgcccggc ggcaagggag gaggcccgag
      121 atcgctcatg gacaccgtgc gtccgaaggg aacgcttcat gggggaaccc ggtgacagac
      181 ctcctggcga taaccgtttc ctctttcttc ctggcctttt ccggcgcagc catgccgggc
      241 cccctcatga ccgccacgat cggggagagc gcacgcaggg gtccggttgc gggcccggcg
      301 ctcgtggcag gccacggcat cctggaactc ttgctcgtga tcctgctcat gctgggtctc
      361 gcgcccttcc tcaagcagga catggtcttc gcggtcatag cgatggccgg tgccctcatg
      421 ctggcaggca tgggcgtctc catgctcctc aaggtccccg ggctcacctt aagccgggaa
      481 gcctcgggcg gcggcatgtc caggctcgtg gccgcagggg cggtcctcag cctttcgaat
      541 ccctactgga cgctctggtg gtccaccatc ggcctcacct acatcctcta cagcgcgcgt
      601 ctcgggaacg cgggcgtggc cctcttcttc gcgggacacc tcttgggcga cctcgcctgg
      661 tacagcgccc tttccttcgc cgtgggaagg gggaggcggt tcatgagcga ccggtcgtac
      721 cgggtgatcg tggccctgtg ctcgttgttc ctggtggtgc ttgccgcggt cttcgccgtg
      781 agcggcctgc gtacgttcct gtccgtctga ggcggtgcag acgggcggtg gccggggctc
      841 ggtcaagccc cctcgggccg cgtctgtgtt ccgactggtg cggcccggtc ctcgcccgtg
      901 cttgggacgt catgggtacc ccgtgacccg ccggcggtgc cgatgcaccc atcgggaagg
      961 catgcacccg gcatggtccg ggagggctcg cgtggagggg gttgcgcgtg gccccgcccc
     1021 gtacgtgcac ggcaacaacg gggcacgggg gaccatgtct ctgggtgcgg ctgcactctc
     1081 gggagggttc atgccgaggc gtgcagagcg ggctgcccgg gcggcgagtc gcgatggcca
     1141 tggcctcgcg ggggtaggaa gcacgtgcga cgcacgacgg gggtgtgccc ggggttgcct
     1201 tcttgcatct gcaccttact gttctgtgag ggatggatcg ctcatgcccg cggtgtctcg
     1261 tgaggagagg tcatggacgc cgaaggcgga aaaagaggaa ggggagggaa accatgaggg
     1321 acagggccgc ggcgtcggtc atggcgtcgt tcatcgggga cgcgctcgcg ctgggcgtgc
     1381 actgggagta ttcgacgagg aagctcaggc aggagttcgg ccgcgtggac tcctacctcg
     1441 acccggggcc ggggtcgtac caccgcacga gaaagaaggg ggagttcacc cactacgggg
     1501 accaggccta cgttctcctg gaatcggtcg catcctcggg cgcgttcgac gccgaagact
     1561 tctccaggag atggcggagg ctgttcgagg attacgacgg gtatctcgac caggcgacga
     1621 ggaccacgct cgagaacctc tcgcgggggg tgcctttcct ggaggccgg
//
LOCUS       DAOSCR010000612         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_48779_length_1667_cov_3.076303, whole genome shotgun sequence.
ACCESSION   DAOSCR010000612 DAOSCR010000000
VERSION     DAOSCR010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48779_length_1667_cov_3.076303"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLT69_12050"
     CDS             <1..328
                     /locus_tag="PLT69_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ15218.1"
                     /translation="ASAFGSQRITVAVDAQRTRARESGCESAVSGGNVTAARHAVARA
                     LQCEELGAGVPLPTSMDGDGTLKGNDIFFTRAVVGEMNIPMVASVGAGTLEDFGKAAK
                     GATRAS"
     gene            493..>1667
                     /locus_tag="PLT69_12055"
     CDS             493..>1667
                     /locus_tag="PLT69_12055"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOJ15219.1"
                     /translation="MKKLRTAAIVFVCLLVVFTITGFYVLPPVLVSVASKNLSKALER
                     RVTIQEIKVNPYTLVVTVKGLSISDLDRHRTFASFDSLMVNAEWASLFKRAPVVREVR
                     LHRPSVRIVRFEDNTYNVSDLLHKDAKKGKSAPMLFSVSNIQVTGGTVIMDDRPVHKI
                     HTAEDISLSIPFISNMPHLTEVFVQPAFHAVINKTPITLTGRSKPFSESLESTLSLDL
                     KDIDIPRYLAYVPAKFGFALESCRADLSATITFRQFKDKRTPESSVSGRMVLSDVVLA
                     ELDGIRLLSIPSLTVDVAASSFLSKEVHVRKIGVHAPVLALRRDRSGTLNLSWALKQT
                     KATGKPPEQPKETPPPEPVLLTIDEISLEKGALSYLDSSGGSPVKIVAADLTARAYD"
BASE COUNT          318 a          543 c          499 g          307 t
ORIGIN      
        1 cgccagtgcc ttcggttcac agaggatcac cgtggccgtc gatgcccaga ggaccagggc
       61 cagggaatcg ggatgcgagt ccgctgtgtc gggcggcaac gtcacggcgg ccaggcacgc
      121 ggtggcccgg gccctccagt gcgaagaact cggcgcaggt gtgccgctgc ccacgagcat
      181 ggacggcgac ggcacgctca agggcaatga catcttcttc acccgggccg tcgtgggaga
      241 gatgaacatc cccatggtgg cgtccgtggg tgcgggaacg ctggaagact tcgggaaggc
      301 cgcgaagggg gccacgcgag catcgtgagc gtcgcccagg tctttttctt caagaccttc
      361 ggcctctccc atgtgatgga cttcctgggt ccccggggca tcgaggtcgc ggcctgagac
      421 tgtttgcaca ccgcccacga gtgcccggcg gcctgagcgc catcgagaca tttccggaaa
      481 gggcgagggg acatgaagaa gctccgcaca gcagccatcg tctttgtctg cctgctggtc
      541 gtgttcacga tcaccgggtt ctacgtcctg cccccggtgc tcgtctcggt ggcgtccaag
      601 aacctctcca aggcgctcga gcgccgggtg accatccagg agatcaaggt caacccctat
      661 accttggtcg tgacggtcaa gggcctttcc atcagtgacc tggacaggca caggaccttc
      721 gcgtccttcg actcgctcat ggtcaatgcc gagtgggcat ccctgttcaa acgggccccg
      781 gtggtcaggg aggtgcggct ccaccgtcct tcggtcagga tcgtccggtt cgaggacaac
      841 acctataatg tttccgatct cctgcacaag gatgcgaaga agggaaagtc agcgcctatg
      901 ttgttctccg tgtcgaacat ccaggtcacg ggcggcaccg tgatcatgga cgatcggccg
      961 gtgcacaaga ttcacacggc cgaggacata tcgctgtcca tcccgttcat ctccaacatg
     1021 ccccacctca ccgaggtctt cgtgcagccc gccttccacg ccgtgatcaa caagaccccc
     1081 ataaccctta ccgggaggtc gaaacccttt tccgaatcac tggaaagcac gctctccttg
     1141 gatttgaagg acatcgacat cccacgatat ctcgcctacg tgcccgcgaa gttcgggttt
     1201 gccctcgagt cgtgcagggc cgatctgagc gcgaccataa ccttccggca gttcaaggac
     1261 aagcgcacac ccgagtccag cgtctccggt cgtatggtcc tgagcgacgt ggtcctggct
     1321 gagctcgacg gcatacggct tttgtccata ccttcactca cggtcgatgt cgcggcctcc
     1381 tcgttcctga gcaaagaggt ccatgtccgg aagataggcg tccacgcgcc ggttctcgcc
     1441 ctcaggagag acaggtcggg cacgctgaac ctgtcttggg ccctgaagca gaccaaggcc
     1501 acgggcaagc cccctgaaca gcccaaggag acgccgccac cggaacccgt gctgctcacc
     1561 atcgacgaga tctccctgga aaagggcgcc ctatcctatc tggacagctc gggcggctcg
     1621 cccgtgaaga tcgtcgccgc agacctgacc gccagggcgt acgacat
//
LOCUS       DAOSCR010000613         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49036_length_1661_cov_1.816314, whole genome shotgun sequence.
ACCESSION   DAOSCR010000613 DAOSCR010000000
VERSION     DAOSCR010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49036_length_1661_cov_1.816314"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1661
                     /gene="selB"
                     /locus_tag="PLT69_12060"
     CDS             153..>1661
                     /gene="selB"
                     /locus_tag="PLT69_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOJ15220.1"
                     /translation="MKRIVIGTAGHVDHGKTSLIRAMTGVDCDRLKEEKERGLTIELG
                     FTSLELPSGEQVGVVDVPGHVKFIRHMLSGASGIDLVMLVVAADEGVMPQTVEHIQIC
                     SLLGIQRGVVVLTKVDMVDDDLFELAHADIREFVEATFLRGAPIVPVSSVTGAGIDEL
                     KKTLDEQIATLKERRITGVPVLPVDRVFTVKGFGTVVTGTMKQGVFQEDQEVEVLPAG
                     RSARVRNIQVHGQETDRAQAGMRTAINLQGFSREEVERGQWVVPVGVFKPTRLMDARL
                     DLLDKPPRGEVKIHLGTAELTGELSMHEVDGLAVARIRLKEPVIAASGDRFIIRSISP
                     AKTIAGGMVINPSPHRRFSPDIARDLLSDERPRQVAGMVRDAGLRGISRKDLAAVFAE
                     EPSSLDKTLSELLSSGRIVRFDPNNDLYVFEANARSLKEMMLAKVKEYHEQHASSSGI
                     SREHLRSSLKGSVDPKLFHKLLTDLVKKGELQEVGPDIRAKGFTPSLGDAMEA"
BASE COUNT          322 a          516 c          554 g          269 t
ORIGIN      
        1 cgcctgtcca tgtccttata ggcggttata cggcctgatg ccccctgacg acaagcccaa
       61 taacgtcaaa ctttcgctcg ggggaataag gccctcctcg agggcaacga tgaattgacg
      121 gcgatagcgg aatctgttta aaccactcca ttatgaagag aatcgtcatc ggcaccgcag
      181 ggcacgtgga ccacgggaag acctcgctca tcagggccat gacgggcgtg gactgcgacc
      241 gcctcaagga ggagaaggag cgcgggctca ccatcgagct cgggttcacc tcgctggagc
      301 tgccctccgg ggaacaggtg ggcgtggtgg atgtgcccgg gcacgtgaag tttatccggc
      361 acatgctctc gggcgcctcg gggatcgacc tggtgatgct ggtggtagcc gcggacgagg
      421 gggtgatgcc ccagaccgtc gagcatatcc agatctgcag cctcctgggt atccagcggg
      481 gcgtcgtggt gctcaccaag gtcgacatgg tggacgacga tctgttcgag ctggcgcatg
      541 ccgatatccg tgagttcgtg gaggctacct tcctccgcgg cgctcccatc gtgcccgtga
      601 gctccgtgac cggagcgggc atcgacgagc tcaagaaaac cctcgacgag cagatcgcaa
      661 cgctcaagga gcggaggatc acgggggtac cggtcctgcc cgtcgaccgg gtattcacgg
      721 tcaagggctt cggaaccgtg gtaaccggga ccatgaagca gggggtgttc caggaggacc
      781 aggaggttga ggtcctgccc gccggccgaa gtgcccgcgt gaggaacatc caggtgcacg
      841 gccaggagac cgaccgggcc caggccggca tgcgcaccgc catcaacctc cagggcttct
      901 cacgcgagga ggtcgagcgg gggcagtggg tcgtgcccgt gggcgtgttc aaacccacga
      961 ggctcatgga cgcccggctc gacctgctgg acaagccccc caggggcgag gtcaagattc
     1021 acctgggaac ggccgagctc acgggcgagc tgagcatgca cgaggtggac ggcctcgccg
     1081 tggcgaggat ccgcctgaag gagccggtca tcgcggcatc tggcgacagg ttcatcatcc
     1141 ggagcatctc cccggcaaag accatcgccg ggggcatggt catcaacccc tcgccgcacc
     1201 ggaggttctc gcccgatatc gccagggacc tgctgagcga cgagaggccg cgccaggtgg
     1261 cgggcatggt ccgcgacgcg ggcttgaggg gcatatctcg gaaagacctg gccgcggtct
     1321 tcgccgaaga gccctcttcc ctggacaaga ccctgtccga gctcctgtct tccgggcgga
     1381 tcgtccgctt cgacccgaac aatgatctct acgtattcga ggcgaacgct cggtcgctca
     1441 aggagatgat gctcgcaaag gtgaaagaat accacgagca gcatgcctcc tcgtccggga
     1501 tctcccgcga gcatctccgc tcgtcactca aggggagcgt ggatcccaag ctcttccaca
     1561 agctcctgac cgacctggta aagaagggcg agctccagga ggtcggcccg gacatccgcg
     1621 caaaggggtt caccccctcg ctgggagacg ccatggaggc g
//
LOCUS       DAOSCR010000614         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49090_length_1659_cov_3.788030, whole genome shotgun sequence.
ACCESSION   DAOSCR010000614 DAOSCR010000000
VERSION     DAOSCR010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49090_length_1659_cov_3.788030"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..779
                     /locus_tag="PLT69_12065"
     CDS             150..779
                     /locus_tag="PLT69_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ15221.1"
                     /translation="MRRPRTSEELERAKDRIIDAALAIIVEEGFPCLTMRRLASRLHM
                     SAPNLYNFFASKDEIYITIVIKGFRMLQEELERAASGASTPEEKARAMIEAYVDFGTK
                     NSPYYDIMFTMPTPKYKAYQGTPYEKLSEVEYRISMDIAGLAAQTLRELSRGGVQKDE
                     DLTHDLVHIWCLVHGMVSLANSNIIGYVASDVKAAYQRIVDDIVERYRG"
     gene            1059..1218
                     /locus_tag="PLT69_12070"
                     /pseudo
     CDS             1059..1218
                     /locus_tag="PLT69_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1415..>1659
                     /locus_tag="PLT69_12075"
     CDS             1415..>1659
                     /locus_tag="PLT69_12075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ15222.1"
                     /translation="MTYEFDGREYEKASAHQQEWGNRLISELNLTGNERILDLGCGPG
                     LYTKRLSDAGYDVTGMDYSRRSIAYAKEHDTETRYIYR"
BASE COUNT          405 a          462 c          480 g          312 t
ORIGIN      
        1 aggcattcac catggggaag gatctcgatg ccttgggggg gttcgcctca cgcgtcgcga
       61 ggcgcacgca ggtgaagatc ggctcgggga tgctcggggg ggtatctcgt gttgacattt
      121 gaggccgttg cggttaaggc ttggtgctca tgaggaggcc caggaccagc gaggagctcg
      181 agcgcgcaaa agaccggatc atagacgccg cgctcgccat catcgtggag gagggtttcc
      241 cctgcctcac catgaggagg ctcgcgtccc ggcttcacat gagcgccccg aacctctaca
      301 acttcttcgc gagcaaggac gagatctaca taaccatcgt gatcaagggg ttcaggatgc
      361 tccaggagga gctcgagcgg gccgcgtcag gcgcatccac gcccgaggag aaggcgaggg
      421 cgatgatcga ggcctacgtg gacttcggga cgaagaacag cccctactac gacatcatgt
      481 tcaccatgcc cacgcccaag tacaaggctt accaggggac gccctacgag aagctctcgg
      541 aggtggagta caggatctcg atggacatcg ccggtctcgc cgcacagacc ctcagggagc
      601 tctcgagggg aggcgtccag aaggacgaag acctcaccca cgacctggtc catatctggt
      661 gcctggtgca cgggatggtc tccctggcca acagcaacat catcggctac gtggcaagcg
      721 acgtcaaggc ggcctaccag cgtatcgtgg acgacatcgt ggagcgatac aggggataga
      781 acccgcttcg agcggacagg cggccttgtt cgaggatccc cggtgcctgc cgggaagaga
      841 acgtccccca ggaagaaagc tgggcagaga acaggtgaga aggctttccc gcccgcccaa
      901 gccgcttgac cgggtgcgcg aagccttgcg ttcccgccac tacaaccccc gcacggagca
      961 gacctcttgc cattgattca gacgccacaa ccgctttcac aatgtgctcc atcccgccga
     1021 aatggccggg ccgggaatca acgccttgtt gacccacctg ccacgcattc cgtcattcct
     1081 tcgccacgca actccttgaa cgcggctacg accatcagga ccgttcaaga actcctcggt
     1141 cacaaggaca tcaaaactcc gatgattcac acccatgtcc tgaaccgggg ctgcaagggc
     1201 gtcaaaagtc cggtggacaa ttgggagagg agaagccaag gtgttttatt tagaagccat
     1261 atatccccct gcgatcattg cagaacatgg cctagcttct gcaagtgctg gagttacggc
     1321 agaactgcag gaggggtctt atgcatcgac gtcgcggccc gaaggtgtta tgaagaaacc
     1381 atataaccat tgttatacaa aagattaata ggttatgact tacgaatttg acggtagaga
     1441 atatgaaaaa gcttcggctc atcagcagga atggggcaat agattaatct ctgaattaaa
     1501 tctcaccggt aatgagagaa tacttgattt aggctgtggt ccgggacttt acaccaaaag
     1561 gctgtcagat gcaggttatg acgttaccgg catggattat tccagacgct cgattgctta
     1621 cgcaaaggaa cacgataccg aaaccagata tatataccg
//
LOCUS       DAOSCR010000615         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49306_length_1654_cov_3.248906, whole genome shotgun sequence.
ACCESSION   DAOSCR010000615 DAOSCR010000000
VERSION     DAOSCR010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49306_length_1654_cov_3.248906"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1452)
                     /locus_tag="PLT69_12080"
     CDS             complement(187..1452)
                     /locus_tag="PLT69_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOJ15223.1"
                     /translation="MDRVFALVDCNNFYASCERVFDPRLAGRPVVVLSNNDGCVIARS
                     EEAKALGIRMGQPFFECAHIARLHGVHVFSSNFPLYGDISRRVMETLSRFTPSIEFYS
                     IDEAFLDLSGMERDLTAYCAAVRDTVLKWVGIPVSIGVGPTKTLAKAAAKAAKRDPSL
                     GGVLDIRGREEETLASLEVEDVWGVGRRHARMLASCGIRTALDLRDAPVDWVRSRMTV
                     TGLRTVMELRGVSCLRLDEVEPARKSIMCSRSFGRKVYALAELEEAASAYASRAAEKL
                     RSQGCAASLVQVVLIEFPFNEGFPATRVCSGELPVATSSTPEFIRAAKALLRRIYVEG
                     PAYWKVAVVLSGIVARGAVQLNLFSPGGRGGKDLALMEAVDTVNRRWGRGTLSFAACG
                     FDRPWWMRQARRSAPFTTSWDHLPTARAI"
     gene            complement(1456..>1654)
                     /locus_tag="PLT69_12085"
     CDS             complement(1456..>1654)
                     /locus_tag="PLT69_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOJ15224.1"
                     /translation="DRALEPKNGSVVIAVVDGEFTVKRYRHSRGRSVLAADNPDFRPI
                     EPAGHMAVEVWGVVTYVIHKE"
BASE COUNT          273 a          562 c          577 g          242 t
ORIGIN      
        1 actccgaagg acagagacct ggatcaaaag tgcgaaacat tcttgacacc acccccaccc
       61 cataggccgg ccgggcgggg gaggtccggg cggccagggc ctctcggccc ttggagggca
      121 cggcaggcag ggtgagcggg ggagatgcgg cgatcggcgc tggggacgaa ggcctcgggt
      181 gacccgtcag atcgccctgg ccgtgggcag gtggtcccac gaggtggtga agggcgcgga
      241 cctcctggcc tggcgcatcc accacggcct gtcgaacccg catgccgcga aggagagcgt
      301 gccgcggccc caccgacggt tcacggtgtc cacggcctcc atgagggcca ggtctttgcc
      361 gcctctgccc cccggcgaga agaggttgag ctgcacggcg cctctggcca cgatgcccga
      421 gagcacgacg gccaccttcc agtaggcggg cccctcgacg tagatgcgtc tcaacagggc
      481 cttggcggcc cggatgaact cgggcgtgga ggaggtcgcg acgggaagct cccccgagca
      541 gacgcgggtc gcgggaaacc cctcgttgaa gggaaactcg atgagcacca cctggacgag
      601 cgaggcggcg cacccctggg acctgagctt ttcggccgca cgcgacgcgt aggcggaggc
      661 ggcctcctcc agctcggcca gcgcgtagac cttcctgccg aaggacctgg agcacatgat
      721 ggacttcctt gccggctcga cctcgtccag ccgcaggcag gagacgcccc tgagctccat
      781 gaccgtgcgc aggcccgtga ccgtcatgcg gctcctcacc cagtcgaccg gcgcgtccct
      841 gaggtcgagc gcggtgcgta tcccgcagga cgcgagcatg cgcgcgtggc gtcggcccac
      901 accccagacg tcttccacct ccagggacgc gagggtctcc tcttccctgc cgcggatgtc
      961 gagcacgccg ccgagcgagg ggtccctctt cgccgccttg gccgccgcct tggccagggt
     1021 cttggtgggt ccgacgccga tggagaccgg gatgcccacc cacttgagca ccgtgtccct
     1081 gacggccgcg cagtaggcgg tcagatcccg ttccatgccc gagaggtcga ggaaggcctc
     1141 gtcgatggag taaaactcga ttgagggggt gaagcgggag agcgtctcca tgacccggcg
     1201 cgagatgtcc ccgtagaggg ggaagttgga cgagaacacg tgcaccccgt ggagccttgc
     1261 gatgtgcgcg cactcgaaga agggctggcc catccgtatg cccagggcct tggcctcctc
     1321 ggagcgggcg atcacgcacc cgtcgttgtt cgagagcacc acgacgggcc tgccagcgag
     1381 ccgcgggtcg aagacccgct cgcaggacgc gtagaagttg ttgcagtcga ccagcgcgaa
     1441 gaccctgtcc atgactcact ccttgtggat cacgtaggtc accacgcccc agacctccac
     1501 cgccatgtgg cccgcgggct cgatgggcct gaagtcgggg ttgtcggcgg cgagcacgct
     1561 cctgccccgg gaatgccggt agcgcttgac cgtgaactcg ccgtcgacga ccgcgatgac
     1621 gacgctgccg ttcttcggct cgagggcgcg gtcg
//
LOCUS       DAOSCR010000616         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49428_length_1651_cov_3.501253, whole genome shotgun sequence.
ACCESSION   DAOSCR010000616 DAOSCR010000000
VERSION     DAOSCR010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49428_length_1651_cov_3.501253"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLT69_12090"
     CDS             complement(<1..711)
                     /locus_tag="PLT69_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOJ15225.1"
                     /translation="MCRIAGIWTPPRPGIAETCVAMRDSMSAGGPDDAGIFVDERAGI
                     ALGHRRLSIIDLTRTGHQPMTSSDGRYTICYNGEVYNYRELREGLEAKGHTFRGTSDT
                     EVVLALFSAYGPTSVESLVGMFAFAVWDGLDRRLFLVRDRLGVKPLYYFHDGETFAFA
                     SELKALHAGLGKGLTVDVDSLAEFLHYGYISGPRSIYRHVRKLEPGCMLAFDRDTAPA
                     VSRYWDPAEHIGPPRRVLD"
     gene            complement(814..>1651)
                     /locus_tag="PLT69_12095"
     CDS             complement(814..>1651)
                     /locus_tag="PLT69_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOJ15226.1"
                     /translation="GGDELFGGYPRYTRALELYRENGLVRRAARIPGLLMFFRLVTPS
                     LLDRLYHRIRPALPGRFRNFKNPGSALHSLLVGEEPMTFERLYLTMMSHWNDPAGIVI
                     SGREPSTAVNDPSRWLDSPSHEQRMMFLDLVTYLPDDILVKVDRAAMAVSLETRVPFL
                     DHRVVEFAWTIPVEKKIRAGQGKWILKKLLGRYVPMPLVDRPKMGFGMPIDAWLRGPL
                     REWAEDLLEEHRLRREGFFNPAPIREKWEEHLEGKSDWHYLLWDVLMFQAWLEEQSRR
                     LT"
BASE COUNT          263 a          530 c          546 g          312 t
ORIGIN      
        1 gtccaggaca cgccttgggg ggccgatgtg ctccgcaggg tcccagtacc tcgagaccgc
       61 gggggcggtg tccctgtcga aggcgagcat gcagcccggc tcgagcttgc ggacgtgcct
      121 gtagatggag cggggcccgg agatgtaccc gtagtggagg aattcggcca gagagtcgac
      181 atcgacggtg agccctttcc ccaggcccgc atgcagcgcc ttgagctcgg aggcgaaggc
      241 gaaggtctct ccgtcgtgga agtagtagag gggtttcacc cccagccggt cgcgtacgag
      301 gaacaggcgc ctgtcgaggc cgtcccacac ggcgaaggcg aacatgccca cgagggactc
      361 gacgctcgtg gggccgtagg ccgagaacaa ggcgagcacc acctcggtgt cggaggtgcc
      421 cctgaaggtg tggccctttg cctcgagccc ttctctgagc tccctgtagt tgtacacctc
      481 gccgttgtag cagatggtgt acctgccgtc tgagctcgtc atcggctggt ggccggtccg
      541 ggtgagatcg atgatggaaa ggcgcctgtg gccgagcgcg atccccgccc tctcgtcgac
      601 gaagatcccc gcgtcgtccg ggccgcctgc gctcatggag tcgcgcatgg cgacgcaggt
      661 ctcggcgatg ccaggacggg ggggtgtcca gatgcccgct atcctgcaca tggcctcgag
      721 acggtcggtg cgccccgtcg gtggccgtgg gccgcacccc cggttgcagg cgtgtgtgat
      781 ggttctctca aggcttcccg ttccatgtgc cggctaggtc agccgcctcg actgctcctc
      841 gagccatgcc tggaacatga gcacgtccca caggaggtaa tgccagtcac tcttgccttc
      901 gaggtgttct tcccatttct cccgtatggg ggcggggttg aagaagccct ccctcctgag
      961 cctgtgctct tcgagcagat cctcggccca ttccctgaga ggccccctca gccacgcgtc
     1021 gatgggcatg ccgaagccca tcttgggcct gtccacgagg ggcatgggca cgtaccttcc
     1081 caacagcttc ttcagtatcc acttgccctg gcccgccctg atcttctttt cgaccgggat
     1141 ggtccaggcg aactccacga ccctgtggtc gaggaagggc acccgggttt cgagcgatac
     1201 cgccatggcc gccctgtcca ccttcacgag gatgtcgtcg ggcaggtacg tgacgaggtc
     1261 gaggaacatc atcctctgct cgtgactggg cgagtcgagc cagcgggacg ggtcgttcac
     1321 ggcggtcgag ggttccctgc cggagatgac gatcccggcg gggtcgttcc agtgggacat
     1381 catcgtcagg tagaggcgtt cgaacgtcat gggttcctcg ccgaccagca ggctgtgaag
     1441 cgctgagccg gggttcttga agttcctgaa tctcccgggc agtgccggcc gtatcctgtg
     1501 gtagagcctg tccagcaggc tcggcgtgac caacctgaag aacatcaaga gcccggggat
     1561 cctcgccgct ctcctcacca agccgttctc cctgtacagc tccagggccc tggtatagcg
     1621 cgggtaaccg ccgaagagct cgtcgccgcc g
//
LOCUS       DAOSCR010000617         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49573_length_1648_cov_2.349027, whole genome shotgun sequence.
ACCESSION   DAOSCR010000617 DAOSCR010000000
VERSION     DAOSCR010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49573_length_1648_cov_2.349027"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLT69_12100"
     CDS             <1..213
                     /locus_tag="PLT69_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ15227.1"
                     /translation="EVDILKGKVRPGDIFLLCSDGLTDMIEDVEIAACLSPPRRLDDM
                     ARDLVDRANRAGGKDNVTVVLAVVLP"
     gene            210..1142
                     /locus_tag="PLT69_12105"
     CDS             210..1142
                     /locus_tag="PLT69_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOJ15228.1"
                     /translation="MRPGPRIPVVLCVALLALAPVTCPPPEAHGLALTRVVQVPRLEA
                     EKAITDWLARSGHEVVRTERGQSVELVATRKGTARRIVLVHHSPLATEVAAPEGGPWA
                     QDLWSFLDAYEETVGGTREYQANTIPEEVRRRSASTVCILAKVRGGPIQLSGFVVDDG
                     GLVLCTAHLLRNPEEITITTASGGRARGVLVKVDYERDLALIDCPGKPGPAVELGGRR
                     GAVLEGEQVYSVGCPGLNGLTINAGTVSGPPRLVNGRVFVQVRMPVSPGSSGSPVFDA
                     AGALVGVVQGRVKPERRMGLVIPMDTVIEFVKER"
     gene            1144..>1648
                     /locus_tag="PLT69_12110"
     CDS             1144..>1648
                     /locus_tag="PLT69_12110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ15229.1"
                     /translation="MNYGRYEIIREIGRGSMGMVYLARDPQIGRDIALKVLRPDRVES
                     EDFVRRFLREARAIGRMSHPNVVTVHDVGEDHGTVYIAMELLEGEPLNVYAAKRTLRL
                     EDIAGIGAQIARCLDYAHGRGIVHRDVKPQNIIVSPDGTVKLTDFGIAHIEDPEAGSQ
                     TQAGEILG"
BASE COUNT          299 a          496 c          613 g          240 t
ORIGIN      
        1 gaggtcgaca tcctgaaggg gaaagtgagg cctggcgaca tcttcctgtt gtgctccgac
       61 gggctgaccg atatgatcga ggacgtggag atagcggcct gcctgagtcc gcctcgtcgt
      121 ctcgacgaca tggcgcggga tctcgtggac cgtgcgaaca gggcgggcgg caaagacaac
      181 gttaccgtgg tcctggcggt ggtgttgccg tgaggccggg cccgaggata cccgtggtgc
      241 tgtgcgtcgc cctcctcgcc ctcgccccgg tgacgtgccc ccctcctgag gcgcacggcc
      301 tggccctcac gagggtcgtg caggtcccgc ggctcgaggc ggaaaaggcg ataaccgatt
      361 ggctcgcccg gtcaggccac gaggtcgtgc gcaccgagcg gggacagtcc gtggaactgg
      421 tcgcaacgag gaaggggacc gcccggcgga tcgtcctggt gcaccactcc ccgctcgcca
      481 ccgaggtcgc ggcgccggag gggggaccgt gggcccagga tctctggtcg ttcctcgacg
      541 cctacgaaga gacggtcgga gggacaagag agtaccaggc caatacgatc ccggaagagg
      601 tgaggcgacg ctcggcgtcc acggtctgca tcctcgcgaa ggtcagaggc gggccgatcc
      661 agctgagcgg gttcgtggtc gacgacggcg gcctcgtcct gtgcactgcg cacctgctca
      721 ggaaccccga agagatcacg atcaccaccg caagcggcgg gagagccagg ggggttctcg
      781 tcaaggtgga ttacgagagg gacctcgcgc tcatagactg ccccgggaag ccggggccgg
      841 ccgtcgaact cggcggaagg cgcggcgcgg tcctcgaggg agagcaggtc tactcggtgg
      901 gctgtcccgg cttgaacggg ttgaccatca atgcggggac ggtgagcggc cccccgaggc
      961 tcgtcaacgg gagggtgttc gtccaggtgc gcatgccggt ttcaccgggc agcagcggga
     1021 gcccagtctt cgacgccgcg ggagcgctcg tgggggtcgt gcaggggagg gtgaagcccg
     1081 agaggaggat ggggctcgtc atccccatgg acaccgtgat cgagttcgtg aaggagcggt
     1141 gagttgaact acgggaggta cgagatcata agggagatcg gcaggggctc catgggcatg
     1201 gtgtacctgg cccgggaccc gcagatcggg cgggacatag cgctgaaggt gctcaggccg
     1261 gacagggtcg aaagcgagga cttcgtcagg cgcttcctca gggaggcgag ggccatcggc
     1321 cgcatgtcgc acccgaacgt ggtgaccgtc cacgacgtgg gggaggatca cgggaccgtc
     1381 tacatcgcca tggaactgct cgagggcgaa cccctcaacg tgtacgcggc gaagaggacg
     1441 ctccgcctcg aagacatcgc gggaatcggg gcccagatcg cccgatgcct ggactacgcc
     1501 cacggcaggg gcatcgtgca cagggacgtc aagccgcaga acatcatcgt gagcccggac
     1561 ggcacggtga agctcaccga tttcggcatc gcccacatcg aggaccctga ggccggttcc
     1621 cagacccagg cgggagagat cctcggga
//
LOCUS       DAOSCR010000618         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49593_length_1647_cov_2.930276, whole genome shotgun sequence.
ACCESSION   DAOSCR010000618 DAOSCR010000000
VERSION     DAOSCR010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49593_length_1647_cov_2.930276"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLT69_12115"
     CDS             complement(<1..842)
                     /locus_tag="PLT69_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ15230.1"
                     /translation="MDKFFNPTSVVVIGASNSPFNLGATICRALNEFSPYPGKVYAVN
                     RKGEDVHGCPGFTRVTDIPDNVDLAVVIAPSAVVPAFLEQCGEKGIRHVVIESSGFSE
                     AGEQGKALQGKIDGIRSRYGIRVMGPNCLGVLNTENKFCCFYGIQKEDFEIFAGESGE
                     VSYIIQSGGIVVIVLESFRTDVVKAAKIVSIGNKSDIDEADMLEYFENDPKTKVIGMY
                     IENVTKGRKFLETARRVTKPVLVFKVGRTQEGVRAALSHTAGMANNDLIFDAACRQAG
                     IIRV"
     gene            complement(892..1434)
                     /locus_tag="PLT69_12120"
     CDS             complement(892..1434)
                     /locus_tag="PLT69_12120"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ15231.1"
                     /translation="MDTEQVILGLLMSGPKSGYKIKNITGRLMMAYNLSLNQIYPALR
                     KLEAANLVKKEVVIQTGKPNMHVYGLTPLGREAFYQAITAPPVPIDYQLDFLTRAFFF
                     RFLGEEEVVRQFEQEISSLEEQLEDLEQIRPEVEARGEEDGLFIFSTIVDLIQTLRDR
                     YTGELARRKKRLAGKGDGMT"
BASE COUNT          282 a          515 c          482 g          368 t
ORIGIN      
        1 tgcacgcgga tgattcccgc ctggcggcag gcggcgtcga agataaggtc gttgttggcc
       61 atgcctgcgg tgtgggacag ggcggccctg accccctcct gggtccttcc gaccttgaag
      121 acgaggaccg gcttggtgac cctgcgcgcc gtctcgagaa acttccggcc cttcgtcacg
      181 ttctcgatgt acatgccgat caccttcgtc ttggggtcgt tctcgaagta ctcgagcatg
      241 tcggcctcgt cgatgtcgct cttgttcccg atgctcacga tcttcgcggc cttgaccacg
      301 tccgtcctga aggattcgag cacgatgacc acgatgccgc cgctctggat gatgtacgac
      361 acctcgcctg actccccggc gaagatctcg aagtcttcct tctggatacc gtagaagcag
      421 cagaacttgt tctcggtgtt tagcacgccg aggcagttgg ggcccatgac cctgatcccg
      481 tagcgcgatc gaatcccgtc gatcttgccc tggagggcct tgccctgctc gcctgcctcc
      541 gagaaccccg agctctcgat cacgacgtgg cggatcccct tttccccgca ctgttcgagg
      601 aaggcgggca cgaccgcaga cggcgcgatg acgacagcca ggtccacgtt gtccggtatg
      661 tcggtgaccc tcgtgaaacc tgggcacccg tggacgtcct cccccttgcg gttgaccgcg
      721 tagaccttcc cggggtacgg cgagaattcg ttcaaggccc tgcagatggt tgcgcccagg
      781 ttgaagggcg agttcgaagc gcctatgacg acgaccgagg tgggattgaa gaacttgtcc
      841 atggatgctc ctttctcgtt gcgtgctctc aattccctgc cggaagcctt gctaggtcat
      901 gccatcacct ttcccggcga gcctcttttt cctccgggcc agttcccctg tgtacctgtc
      961 cctgagggtc tgaatgagat ccacgatggt cgagaagatg aagaggccgt cctcctctcc
     1021 ccttgcctcg acctcgggcc ttatctgctc gagatcctcg agctgctcct cgagcgagga
     1081 gatctcctgc tcgaactgcc ggacgacctc ctcttcgccg aggaacctga agaagaacgc
     1141 cctggtgagg aagtccagct ggtagtcgat ggggacgggc ggcgccgtga tcgcctggta
     1201 aaacgcctcc ctccccaggg gggtgaggcc gtagacatgc atgttcggct tgcccgtctg
     1261 gatgaccacc tctttcttca cgaggttcgc agcctcgagc ttcctgagcg cggggtagat
     1321 ctggttcagg ctcaggttat aggccatcat gagccttccc gtgatgttct tgatcttgta
     1381 gccgctcttg ggccccgaca tgagcaggcc caggatcacc tgttcggtat ccatcacgca
     1441 cccctgcgca gggacgaagg cctcgtagtt tcgtctacca tattgtagaa gaaaatatta
     1501 gaaaatcttt gtgttgtcaa gcattgtctt gcctccccac gtatgattgc cttacacccg
     1561 gcgtacctct tacgggaggg aggcgcgaag ggagtgaaga gagggcacgc ccggggtgtg
     1621 ggccccccgg gatgacccac acccttg
//
LOCUS       DAOSCR010000619         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49630_length_1646_cov_4.174104, whole genome shotgun sequence.
ACCESSION   DAOSCR010000619 DAOSCR010000000
VERSION     DAOSCR010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49630_length_1646_cov_4.174104"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLT69_12125"
     CDS             complement(<1..568)
                     /locus_tag="PLT69_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15232.1"
                     /translation="MVHAATLARVYLLAVLPCLETLSELDPRTQAIAGAWNGDIRLSV
                     AARGPHCTVGIRGGRVAVFDRALAGPDVALFFPHPLFLNNLFKGKHLVVALPWKGLTR
                     VKGLLVFSRLASRMQAVLEGRAGSPQLRAGLTIGLLARSLAVAATWDPLFKQDASRLE
                     GVAEFSIRGGPGAHVHFQGGKATAYPGHA"
     gene            complement(714..>1646)
                     /gene="ychF"
                     /locus_tag="PLT69_12130"
     CDS             complement(714..>1646)
                     /gene="ychF"
                     /locus_tag="PLT69_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOJ15233.1"
                     /translation="LARIVKTETVVPTTLKLFDIAGLVKGASEGEGLGNQFLGHIRGV
                     DAIVHVVRCFEDPQVSHVYADIDPVRDLEIVLAELVMADMDLVERRMETLHKGMRLGR
                     KDMEAGELELLESMKAFLSKGVCVVDSGLEYDQSRMKAWGILTAKPYLVVANVGENDL
                     IQPSKALEALTSWGDSRGKKVIPVCTRFELEASELEEDERREFLSSIGIEKSGTVALI
                     EACYELLDLVTFYTPVGKTLRAWTLKKGGTAFDAAGLIHTDIQQGFIKAEIVSLTDFL
                     SHGGLHGAREAGCVLTEGRDFVLRDKDVLIVHFR"
BASE COUNT          282 a          552 c          511 g          301 t
ORIGIN      
        1 acgcatgtcc cgggtacgcc gttgccttcc ctccctggaa gtgcacgtgc gcgccgggcc
       61 cgcctctgat cgagaactcc gcgacaccct cgagcctcga tgcatcctgc ttgaacaggg
      121 ggtcccaggt cgccgccacc gcaagcgagc gcgccaggag gcctatcgtg agaccggccc
      181 tgagctgtgg cgacccggcg cggccctcga gcaccgcctg catgcgcgat gcgagcctcg
      241 aaaagacgag gagccccttg accctcgtca ggcctttcca tggcagagcg acgacgaggt
      301 gtttcccctt gaagagattg ttcaggaaga gcgggtgcgg gaagaagagg gccacgtccg
      361 ggcctgcgag cgcccggtcg aacacggcga cacgcccccc tctgatcccc acggtacagt
      421 gcgggcctct tgcggccacg ctgagcctga tgtccccgtt ccacgcgccc gcgatggcct
      481 gcgtccgggg atcgagctcg ctcagggtct ccaggcacgg caggacggcg aggaggtaga
      541 cccttgccag ggttgcggca tgcaccatcg gcgatcacct ttcaggggtg cctggtaagg
      601 cgatatgaca tcgagtataa ccacgtgcgg tcattgatgc aacagggttt cgcaggtggg
      661 tccgcaaacg gcacggggtg tgcggatcgt gccttgggtt cggggaagca gggtcacctg
      721 aagtggacga tgagaacgtc cttgtccctc agcacgaagt cccttccttc cgtgagcacg
      781 cagcccgctt ccctcgcgcc gtggagaccc ccgtgggaga ggaagtcggt caaggacacg
      841 atctcggcct tgatgaagcc ctgttggatg tccgtgtgga tgagcccagc tgcgtcgaag
      901 gccgtgcccc ccttcttgag tgtccacgcc ctgagcgtct tccccacagg ggtgtagaag
      961 gtgacgaggt ccaggagttc gtagcacgcc tctatcagcg caaccgtgcc agacttctcg
     1021 atgccgatcg acgagaggaa ctccctccgc tcgtcttctt ccagctccga ggcctcgagc
     1081 tcgaaccggg tgcacacggg tatcaccttc ttgcccctgg agtcccccca tgacgtgagc
     1141 gcctcgaggg cctttgacgg ctggatgagg tcgttctccc ccacgttggc caccaccagg
     1201 tagggcttgg cggtgagtat cccccacgcc ttcatcctcg actggtcgta ctcgagcccc
     1261 gagtcgacga cgcagacccc cttggacaga aaggccttca tggactcgag gagctccagc
     1321 tcccccgcct ccatgtcttt cctgccgagc ctcatcccct tgtgcagggt ttccatccgt
     1381 ctctccacga gatccatgtc cgccatgacg agttccgcga gcacgatctc gagatcgcgc
     1441 acagggtcga tatccgcgta gacgtgtgac acctgggggt cctcgaagca cctcaccacg
     1501 tggacgatgg cgtccacccc ccgaatgtgg cccaggaact ggttgccgag cccttcgccc
     1561 tcggacgcgc ccttgacgag accggcgatg tcgaagagct tgagggtggt ggggaccacg
     1621 gtctccgtct tgacgatcct cgcaag
//
LOCUS       DAOSCR010000620         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49634_length_1646_cov_2.974230, whole genome shotgun sequence.
ACCESSION   DAOSCR010000620 DAOSCR010000000
VERSION     DAOSCR010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49634_length_1646_cov_2.974230"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLT69_12135"
     CDS             complement(<1..837)
                     /locus_tag="PLT69_12135"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOJ15234.1"
                     /translation="MDETRDTDLARLMEQAGVSGVEELHGHLPAGVLLGRPLDLEGPV
                     DELSLRRRFTASPSRVCFAGGGIYHHHIPAVVDAVASRQEFLTAYTPYQPEISQGTLQ
                     AAFEFQSMMASLAGMEVSNASMYDGATALAEAALMALRTGGITRMAVARSVNPAYRKV
                     LCTYLRAMPGVKVEEIPYDAATGRLDAQAARDLAGKDAALFVQQPNYFGVIEDMTEVG
                     AAASGAGFFGVVVAEAVSLGILPAPGGTGCDVVVGEAQSLGNPPSAGGPLLGFFCARK
                     KHV"
     gene            complement(844..1245)
                     /gene="gcvH"
                     /locus_tag="PLT69_12140"
     CDS             complement(844..1245)
                     /gene="gcvH"
                     /locus_tag="PLT69_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOJ15235.1"
                     /translation="MDTLIPEGLFYTKDHEWARVEDGKARVGITDYAQHELGDIVYVE
                     LKPAGTTVKTGDALGTVESVKAVSEVYSPVGGVVAEVNVELDGSPELVNQDCYGKGWM
                     VVIAFEGAGPSKTAGIMDAAAYKEYLAKEAK"
     gene            complement(1288..>1646)
                     /locus_tag="PLT69_12145"
     CDS             complement(1288..>1646)
                     /locus_tag="PLT69_12145"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOJ15236.1"
                     /translation="FAVDLNKGDFMGRSALLEQLRQGIGRKLSGVEITDRGVPRSGCR
                     VFKGGRVVGAVTSGSVSPVTGYGMALAYLEPGLCEGETLEVEVRDKMLASRVKKPPFA
                     PATIEEGEALFTHGTR"
BASE COUNT          243 a          590 c          520 g          293 t
ORIGIN      
        1 cacgtgcttc ttccgtgcgc agaagaaccc caacaggggc ccgccggcgc tcggcgggtt
       61 tccgagagac tgggcctcgc ccacgaccac gtcgcagccg gtaccgccgg gggcgggcag
      121 gatgccgagg gagacggcct ccgccacgac gacaccgaag aagcctgcgc ccgatgcggc
      181 ggcgccgact tccgtcatgt cctcgatgac gccgaagtag ttcggctgct ggacgaacag
      241 ggccgcgtcc ttccccgcaa ggtcccgggc cgcctgggcg tcgagcctgc cggttgccgc
      301 gtcgtacggg atctcctcca ccttcacccc gggcatggcc ctgaggtacg tgcacaacac
      361 cttcctgtag gcggggttca ccgagcgcgc gacggccatg cgcgtgatgc cccccgtgcg
      421 cagcgccatg agggccgcct cggccaaggc ggtcgccccg tcgtacatgg aggcgttcga
      481 gacctccatg cccgcaagcg aggccatcat gctctggaac tcgaaggccg cctggagcgt
      541 gccctggctt atctcgggct ggtacggcgt gtaggcggtg aggaactcct ggcgggacgc
      601 cacggcgtcc accaccgcgg ggatgtggtg gtggtagata ccacctcccg cgaagcacac
      661 cctcgagggg gaggccgtga acctcctcct caggctgagc tcgtccacag ggccctcgag
      721 gtcgaggggg cggccgagga ggacgcctgc ggggaggtgc ccgtggagtt cttccacacc
      781 cgagaccccg gcctgctcca tgagccttgc caggtccgtg tcacgcgtct cgtccatgcc
      841 cggctacttg gcctccttcg cgaggtattc cttgtacgct gcggcgtcca tgatgcccgc
      901 ggtcttgctg ggccctgccc cttcgaacgc gatgaccacc atccacccct tgccgtagca
      961 gtcctggttc acgagctcgg gggaaccgtc gagctccacg ttcacctcgg cgacaacgcc
     1021 gcccacgggc gagtagacct cggagaccgc cttgaccgac tcgacggtgc cgagggcgtc
     1081 gccggtcttc accgtggtgc ctgcaggctt gagctccacg tagacgatgt ccccgagctc
     1141 gtgctgcgcg tagtcggtga tgcccacgcg ggccttcccg tcctcgaccc tggcccactc
     1201 gtggtctttc gtgtagaaca acccttcggg aatgagcgta tccatctgtg cccctctctt
     1261 tttatgtctt cttggggttg agcccgttca acgtgtgccg tgcgtgaaca gggcctcacc
     1321 ctcttcgatc gtggcgggtg cgaacggggg tttcttcacc ctggaggcga gcatcttgtc
     1381 cctcacctcg acctcgaggg tctcgccctc gcagagcccc ggctccaggt aggccagggc
     1441 catgccgtat cccgtaacgg gcgacacgct ccccgaggtc accgcgccca cgaccctgcc
     1501 tcccttgaag actcgacagc cggacctagg cacccccctg tcggtgatct cgacgcccga
     1561 gagcttcctg ccgatgcctt gccgcagctg ctcgagaagg gcgctccttc ccatgaaatc
     1621 acccttgttc aggtcgaccg cgaagt
//
LOCUS       DAOSCR010000621         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49676_length_1645_cov_3.349057, whole genome shotgun sequence.
ACCESSION   DAOSCR010000621 DAOSCR010000000
VERSION     DAOSCR010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49676_length_1645_cov_3.349057"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..609
                     /locus_tag="PLT69_12150"
     CDS             73..609
                     /locus_tag="PLT69_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15237.1"
                     /translation="MPETSPGKRRYLACADYEMSRIAAIERATLVESALTAVFFFLCS
                     LPFVFVSWRFFGQTAARLRVMNEEIARHRDRLEELVHVRTMELEEANERLKHELGERK
                     RAEESLSVQKRELEDALAQVRVLSGLIPICSSCKKIRDDKGYWNQIERYIQEHSDAQF
                     SHSICPECLRRLYPDIKT"
     gene            680..>1645
                     /locus_tag="PLT69_12155"
     CDS             680..>1645
                     /locus_tag="PLT69_12155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOJ15238.1"
                     /translation="MRVALVQLPLQSHDYAYSLENVPLASGYLASRLARCGAAKIHVE
                     ICPMEVANLGGDAAIEEWIVQTRPDVVGMSCSVWNVERSLHICARTARRLGGVTFVMG
                     GPEVAVDNHLLLRHEAFSLGVTGEGEDTFLELVESLREGAEVPGGIPGLLAKREGRIQ
                     WTGPRPLVGRLDDIASPYLSGLLQVSFAGTMLMETLRGCPMRCTYCHYHKSAPFVRAF
                     SLERVAREVAWGRERGVGEITLVDPCFAKRPGVLELLEAVVREGSDELALSLELNAED
                     VSPGLVDALAKARLVHVEVGLQSVNEATLATVGRKFDRDAFTRGVR"
BASE COUNT          280 a          512 c          578 g          275 t
ORIGIN      
        1 gaggcgtacg cgagcggcaa ggtcgcatac gtgaactact cggaccggtg gggtaccttc
       61 aggtccgtgg ccgtgcccga gacctccccc ggcaagaggc gctacctcgc ctgcgcggac
      121 tacgagatga gccgcatcgc ggccatcgag cgcgccaccc tcgtcgaatc cgccctcacg
      181 gcggtgttct tcttcctctg ctcgctgccg ttcgtgttcg tgtcctggag gttcttcggc
      241 cagaccgccg cccgcctgag ggtcatgaac gaggagatcg cccgccacag ggatcgtctc
      301 gaggagctgg tgcacgtgag gaccatggag ctcgaggagg cgaacgaaag gctcaagcac
      361 gagcttggcg agcgcaagag ggcggaggag tcgctctccg tccagaaacg cgagctcgag
      421 gacgcgctcg cgcaggtgag ggtgttgagc gggctcatcc ccatctgctc ctcgtgcaag
      481 aagatccggg acgacaaggg atactggaac cagatcgagc gctacatcca ggagcactcc
      541 gacgcacagt tctcgcacag catctgcccg gagtgcctca ggcgcctcta tccggacatc
      601 aagacgtgac gtgcatgagg gttcgataca ggtatcccgt ctacggcgca ggtcaaaccc
      661 cggccggggg gcaagggcga tgagggtcgc gctcgtccag cttcctctcc agtcgcacga
      721 ctacgcctac tccctcgaga acgtcccgct cgcatccggt tacctcgcct ctcggctggc
      781 aaggtgcggc gcagcgaaga tccatgtgga gatctgtccc atggaggtcg cgaacctcgg
      841 gggggatgcc gccatagagg aatggatcgt ccagacgcgg cctgacgtgg tgggcatgag
      901 ttgttccgtg tggaacgtcg agcgctccct gcatatctgc gcgaggacgg cgaggcgctt
      961 gggcggcgtc accttcgtga tgggggggcc tgaggtggcc gtcgacaacc acctcctcct
     1021 tcgccacgag gccttctccc tgggagtgac aggggagggc gaggatacct tcctggaact
     1081 cgtggagtct ctccgcgagg gggccgaggt gccgggcggg atccccgggc tccttgcgaa
     1141 gcgggaggga cgcatccagt ggaccggccc caggcctttg gtggggaggc tcgacgacat
     1201 agcctctccg tacctctcgg ggctcctcca ggtatcgttc gcaggcacga tgctcatgga
     1261 gaccctcagg gggtgcccca tgcgctgcac gtactgccac taccacaaga gcgcgccctt
     1321 cgtccgcgcc ttctccctcg agagggtggc aagggaggtg gcctggggga gagagagggg
     1381 tgtgggcgag atcacgctgg tggacccgtg cttcgcgaag aggccggggg tgttggaact
     1441 gctcgaggcc gtggtccggg aggggtcgga cgagcttgcc ctgagcctgg agctcaacgc
     1501 cgaggacgtg agtccaggcc tcgtggacgc cctcgcgaaa gcccgcctcg ttcacgtgga
     1561 ggtcgggctc cagagcgtga acgaggcgac gctcgccacc gtgggccgca agttcgacag
     1621 ggacgccttc accaggggtg tcagg
//
LOCUS       DAOSCR010000622         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_49694_length_1645_cov_2.033962, whole genome shotgun sequence.
ACCESSION   DAOSCR010000622 DAOSCR010000000
VERSION     DAOSCR010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49694_length_1645_cov_2.033962"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>454
                     /locus_tag="PLT69_12160"
     CDS             <1..>454
                     /locus_tag="PLT69_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOJ15239.1"
                     /translation="GARALAVMKHVGVNVAADPLMTLPYTLVRGGIVLVSADDPELFS
                     SQNEQDNRHYARFAKIALLEPSSSQEAKDFTVEAFKISEEFGTPVMLRTTTRISHSMG
                     VVELGEASPMKAPTLERHPAEWVMLPANARRRHPVIEERILALKKFAET"
     assembly_gap    455..464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <465..1522
                     /locus_tag="PLT69_12165"
     CDS             <465..1522
                     /locus_tag="PLT69_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ15240.1"
                     /translation="MSDASLGIIASGVAYQYAKEAFPNASFLKLGMPYPLPERLVRDF
                     ASRVKDLWIIEELDPFVEEMVKALGIPCRGKERVPLCGELNPDIVKAAVLGLEIPRPA
                     PQALPPRPPSMCPGCPHRAMLYALKRNKCFIAGDIGCYTLGFLPPLDAIDTCLCMGAG
                     INHAHGMSKVFGPGEQKIAAVIGDSTFFHSGITGLANLAYNSGCALVVILDNRTTAMT
                     GSQNHPGTGVTISGEKTTDIDLESLVKALGIPWVRTVNPYAVNETRQAVKEALDSKGP
                     AVIISRAPCVLLKTRAVPGTVLEVDPETCTGCKVCISLGCPALEFDGEKAHINGVCVG
                     CNVCAEICPAGAIGGGS"
     gene            1519..>1645
                     /locus_tag="PLT69_12170"
     CDS             1519..>1645
                     /locus_tag="PLT69_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ15241.1"
                     /translation="MNVLMTGVGGQGIILASDILSEVVMRAGFDVKKSEIHGMAQR"
BASE COUNT          298 a          517 c          551 g          269 t
ORIGIN      
        1 ccggggcgag ggcgctcgcg gtgatgaagc acgtgggggt caacgtggcc gccgacccgc
       61 tcatgaccct gccctacacg ctcgtcaggg ggggtatcgt gctcgtgagc gccgacgacc
      121 cggagctctt ctcctcccag aacgagcagg acaacaggca ctatgcgcgc ttcgcgaaga
      181 tcgccctgct cgagccctcg agttcgcagg aggcgaagga cttcacggtc gaggcgttca
      241 agatcagcga ggagttcggt accccggtga tgctcaggac caccacgcgg atctcgcact
      301 ccatgggcgt ggtcgaactc ggggaggcgt cgccgatgaa ggcgccgacc ctggagaggc
      361 accccgccga gtgggtgatg cttcccgcaa acgccaggcg gagacacccg gtcatagagg
      421 agcgtatcct ggccctcaag aagttcgcgg agacnnnnnn nnnnagatga gcgacgcctc
      481 gctgggcatc atagcctcgg gggtggccta ccagtacgcc aaggaggcgt tcccgaacgc
      541 atccttcctg aagctcggca tgccgtaccc cctgcccgag aggctcgtcc gcgactttgc
      601 gtccagggtg aaggacctgt ggatcataga ggagctggac ccgtttgtgg aggagatggt
      661 caaggccctg ggcataccgt gcaggggaaa ggagagggtc cctctctgcg gcgagctgaa
      721 ccctgacatc gtgaaggctg cggtcttggg cttggagatc cccaggcccg ccccgcaggc
      781 cctgcctccc aggccgcctt ccatgtgccc ggggtgtccc cacagggcca tgctgtacgc
      841 gctgaagagg aacaagtgct tcatcgccgg cgacatcggc tgctacaccc tcggctttct
      901 gccgccgctc gacgccatag acacctgcct gtgcatgggc gcgggcatca accacgcgca
      961 cggcatgtcc aaggtcttcg ggccgggcga acagaagata gccgcggtca tcggcgactc
     1021 caccttcttc cactcgggca tcacggggct ggccaacctc gcctacaaca gcgggtgcgc
     1081 cctggtggtg atcctggaca accgtaccac ggcgatgacc gggtcgcaga accatcctgg
     1141 gacgggcgtc accatctcgg gcgaaaagac gacggacata gacctcgaat ccctggtgaa
     1201 ggctctgggg atcccgtggg tccgcaccgt caacccgtac gccgtgaacg agacccggca
     1261 ggcggtgaag gaggcgctcg actcgaaggg cccggcggtg atcatatccc gcgcgccgtg
     1321 cgtcctcctc aagaccaggg ctgtgcccgg gaccgtgctc gaggtggacc ctgagacctg
     1381 caccgggtgc aaggtctgca tctcgctcgg gtgcccggca cttgaattcg acggtgagaa
     1441 ggcgcatatt aacggggtct gcgtggggtg caacgtgtgc gccgagatct gccctgcagg
     1501 ggcaatcgga ggtgggtcat gaacgttctc atgacgggcg tgggcggcca gggcatcatc
     1561 ctggcttccg acatcctctc cgaggtcgtg atgcgcgcag gtttcgatgt caagaagtcg
     1621 gagattcacg gcatggccca gaggg
//
LOCUS       DAOSCR010000623         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_50000_length_1638_cov_3.176879, whole genome shotgun sequence.
ACCESSION   DAOSCR010000623 DAOSCR010000000
VERSION     DAOSCR010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50000_length_1638_cov_3.176879"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1316)
                     /locus_tag="PLT69_12175"
     CDS             complement(264..1316)
                     /locus_tag="PLT69_12175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ15242.1"
                     /translation="MQVPARQGVKAAISVIIPMYNEEANAWETVSRVRKLLEEAGRPF
                     EIVTVNDGSTDGTLDVLDRLAQEDGRVRVVSYWKNGGRGKALRYGFAAARCPYVATID
                     ADLSYDPLYILEMARVLDEENDVDMVLVSPYMEGGKAVGVPAARLLVSWLGNKVLQLA
                     MPQKIHTLTCIVRCYRKEVLDSLDLESDGKEIHLEILSKALAMGYHIREIPAVLTSRK
                     KGSSKFSFRSTAVSHLLFTIFERPTLLFGFLGVLAVLTGIILGLYVVYLYTHGLLNPN
                     RPLITLMVLFLLGGVQILSFGFLATQVHFLRKEVLLGRRAIARLRRIALGEDPGMHAG
                     SNRTAAPGKEDVAPPA"
BASE COUNT          256 a          535 c          562 g          285 t
ORIGIN      
        1 ggcgcggcgt cataccgaag acccgggtgt gcgctgtctc tcggtcaggg gagcgagggc
       61 cgtcctgtcg cctcgaggag acgcccggag gggatggctg cgcgcccctg ccccgcactc
      121 tgcgccgcgg cgtcatggtc gcagcagggg cttgcccgac gggcggccgg tccacctctt
      181 gcggacccta tgacacgcgc cggggattgc atcttcatgg cgatggggca cacgggctga
      241 cggtccacct cttgctgacc ctatcacgcg ggtggggcga cgtcttcctt tcccggcgcg
      301 gccgtcctgt tcgaccccgc atgcatgccg ggatcttccc ccagggcgat gcgcctgagg
      361 cgggcgatgg ctctgcggcc gaggaggacc tccttgcgga ggaagtgcac ctgcgtggcc
      421 agaaacccga aggagagtat ctggacgccg ccgaggagga acaggaccat gagggtgatg
      481 agggggcggt tcgggttcag gagaccgtgc gtgtagaggt agaccacgta caggcccagg
      541 atgatccccg tgaggacggc cagcacgccc aggaagccga agagcaaggt cggcctctcg
      601 aatatggtga agaggaggtg ggagaccgcg gtcgacctga acgagaactt ggaggacccc
      661 ttcttcctgg aggtgagcac ggccggtatc tccctgatgt ggtaccccat ggccagggcc
      721 ttggagagta tctccaggtg gatctccttg ccgtccgact cgaggtcgag cgaatcgagg
      781 acctccttcc ggtagcatct cacgatgcac gtgagggtgt ggatcttctg gggcatcgcg
      841 agctggagga ccttgttgcc gagccaggaa accaggagcc gggccgccgg tacgccgacc
      901 gccttccctc cctccatgta cgggctcacg agaaccatgt ccacgtcgtt ttcctcgtcg
      961 agcacgcggg ccatctcgag gatgtagagg gggtcgtagg agagatccgc gtcgatggtc
     1021 gccacgtagg ggcaccgggc cgccgcgaaa ccgtacctga gcgccttgcc cctccccccg
     1081 ttcttccagt aggacaccac cctgacgcgc ccgtcctcct gcgcgagcct gtccagcacg
     1141 tcgagggtcc cgtccgtgct cccgtcgttc accgtgacga tctcgaacgg gcggcccgcc
     1201 tcctcgagga gtttccggac gcgcgagacg gtctcccacg cgttggcctc ctcgttgtac
     1261 atggggatga tcacgcttat cgcggccttg actccctgcc tcgcgggcac ctgcataccg
     1321 tctcgaacca tggggggatc ataaccgaga acggtccttt tgtacacctc ttccccgtct
     1381 gggcgagcgg ctggaatgta tccggcgggt gcgggcgctc gacctcccgc gcgaggccgg
     1441 gcggccgcgg gcaagaccac gggggcggcc ctgtcgggcg gcggcgaggc tcgttgaggt
     1501 ccccacgggg tgcccgccgg tcacccgcga cgaggcgtcg tcagatcagg ctcgtggaga
     1561 aatacctctc ggcccggtcg ggcaggacgg ttgcaatgat cttgtccctg ccgtacttgt
     1621 tcgccatctg caccaccg
//
LOCUS       DAOSCR010000624         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_50148_length_1635_cov_2.986709, whole genome shotgun sequence.
ACCESSION   DAOSCR010000624 DAOSCR010000000
VERSION     DAOSCR010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50148_length_1635_cov_2.986709"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..>1635
                     /locus_tag="PLT69_12180"
     CDS             310..>1635
                     /locus_tag="PLT69_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOJ15243.1"
                     /translation="MNVLRDVSDRGVLVVDDLPFDTVTPVGAYIALVGRSQGFLLESV
                     PVAYSFVGRFAEDGVVELDAGQADCWSRIPLGAPLDFASANGTLPPFIGGYVGYLGYD
                     MVRGVERLPRPKEPSGFPDALLGRADSVLVFDHLRQRLRLLSVIPEPGVSDEEALRRG
                     RESFDEVESALRGTSIDSTPRQVSIRSVKDVSPDEREFTRAVLSAKEYLRRGDIMQVV
                     LSRRMGVEIEGDPFDIYRRLRRINPSPYLFYINLRGVTLIGSSPEEMVRLAGDTVTTV
                     PIAGTRPRGADPEEDDALAAELMSDEKERAEHLMLVDLARNDLGRVCVPGSVRVTARE
                     KVRFYSHVMHIVSLVEGTRRKGVSAVDVLKACFPAGTVSGAPKVRAMEIIDELEGRCR
                     GPYAGAVGYLSHNGNMDTGIVIRTVFMRSGTAYLQAGAGIVWDSTPKREL"
BASE COUNT          293 a          478 c          586 g          278 t
ORIGIN      
        1 gcgctcggcg ggagcccgta tcgaaacggg tcttcacgtc ccgggccgcg cgtgagaggg
       61 ggtgacggcc tcgggaaaaa atctctcaag aaaaaaggct tgacagccga tgagtgtttc
      121 tatatagaga aacaccatgg tgaacgtacg agtggaccag aagaagatca ggtggtggtg
      181 gcgcgccgga aatactggtg tgggaggagg tctgccctag ggtacagtgt tcggccacac
      241 gttgaagcgg caagctcctc cgagagcttg ccgctttttt tttcaggttc gggaaaggtg
      301 atgggacaga tgaacgtatt gagagacgta tcggacaggg gggtgctcgt cgtggacgac
      361 ctgcccttcg acacggtgac ccccgtgggg gcgtacatag ccctggtggg ccggtcgcag
      421 gggtttctcc tggagtcggt ccccgtggcg tacagcttcg tgggcaggtt cgcagaagac
      481 ggcgtcgtgg agctggatgc cggacaggcc gattgctggt cgaggatccc gctgggcgct
      541 cccctggact tcgcctccgc gaacggcacg ctcccgccgt tcataggcgg ctacgtgggg
      601 tacctcgggt acgacatggt gaggggcgtg gagaggctgc cgaggccgaa ggagccttcc
      661 gggttcccgg acgcgctcct ggggagggcg gattcggtcc tcgtgttcga tcacctgcgc
      721 cagcggctca ggctgctctc ggtcatacca gagcccggcg tatccgacga ggaggcgctc
      781 cgtcgcggcc gggagtcctt cgacgaggtg gagtccgcgc ttcgggggac gagcatcgac
      841 tccacgccgc gacaggtcag catacggagt gtcaaggacg tatcgccgga tgagagggaa
      901 ttcacccggg ctgtgctctc cgcaaaggag tacctccgcc gcggagacat catgcaggtg
      961 gtcctctcca ggaggatggg cgtcgagatc gagggggacc ccttcgacat ctaccggagg
     1021 ctcaggcgca taaacccctc cccctacctc ttctacatca acctccgtgg agtcaccctc
     1081 ataggttctt cgcccgagga gatggtgagg ctcgcgggcg acaccgtcac cacggtgccc
     1141 atagccggca cacggccgcg gggggccgac cccgaagagg acgatgcgct cgcggcggag
     1201 ctcatgtccg acgagaagga aagggccgag cacctcatgc tcgtggacct cgcgagaaac
     1261 gacctgggga gggtctgcgt gccggggtcg gtccgcgtca cggcgcgcga gaaggtgcgc
     1321 ttctactccc acgtcatgca catcgtctcc ctcgtggaag gcacgaggcg caaaggcgta
     1381 tccgccgtcg acgtcctcaa ggcctgtttc ccggcgggga cggtgtcggg cgcgcccaag
     1441 gtgagggcca tggagatcat cgacgagctg gaaggccgct gccgggggcc gtacgcgggg
     1501 gcggtcggct acctctccca caacggcaac atggacacgg ggatcgtcat caggacggtg
     1561 ttcatgcgtt cgggcacggc atacctccag gcgggcgcgg gcatcgtgtg ggactcgacg
     1621 ccgaagcggg agttg
//
LOCUS       DAOSCR010000625         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_50435_length_1629_cov_2.590216, whole genome shotgun sequence.
ACCESSION   DAOSCR010000625 DAOSCR010000000
VERSION     DAOSCR010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50435_length_1629_cov_2.590216"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="lnt"
                     /locus_tag="PLT69_12185"
     CDS             <1..1046
                     /gene="lnt"
                     /locus_tag="PLT69_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOJ15244.1"
                     /translation="LEFAQVAELGGVFLVGAVVMAFNVALFQAVRGRYAGLVSALVVV
                     CACTVFGWARLHFTDFEGPGLTVGVVQANVPQDEKWRPERTDEVLGTYLELSRKALSE
                     GARLVVWPETACTFYLFRQWPQTERVLELSRQVDAGFVVGSPAYEDGRFYNRAYLLRR
                     GRIEGFYDKVHLVPFGEYLPFERQLRRFFGSLTSEVGDFVPGQSLRPVGDLGVLVCFE
                     SVFPGLARTLVRSGARLIVNISNDAWFKTWSTPWQHLQQACFRSIETRRYMVRSVNHG
                     ISAVVDPFGRVKGSIGLLEEGVIVAEVEMIDFMSLYTRFGPLVPFLWAVSSTAWALAS
                     LTRARPGGKARNS"
BASE COUNT          276 a          502 c          574 g          277 t
ORIGIN      
        1 tcctggagtt cgcgcaggtg gccgagctgg ggggggtgtt cctcgtgggc gccgtcgtga
       61 tggccttcaa cgtcgccctc ttccaggccg tgaggggccg gtacgcgggt ctcgtgtcgg
      121 ccctcgtcgt ggtgtgcgca tgcacggtgt tcgggtgggc gaggctccac ttcacggact
      181 tcgagggacc cggcctcaca gtgggcgtcg tccaggcgaa cgtcccccag gacgagaagt
      241 ggaggcccga acgcaccgac gaggtgctcg ggacctacct cgagctctcg aggaaggccc
      301 tgtcggaagg ggcccggctc gtggtgtggc ccgagaccgc ttgcaccttc tacctgttca
      361 ggcagtggcc ccagaccgaa agggtcctcg agctgagcag gcaggtggac gcgggcttcg
      421 tggtgggaag cccggcctac gaggacgggc ggttctacaa ccgtgcctac ctcttgagga
      481 gggggaggat cgagggcttc tacgacaagg tgcacctcgt acccttcggg gaatacctgc
      541 cgttcgagcg ccagctgaga cgtttcttcg ggtcgcttac ctccgaggtc ggggacttcg
      601 tgcccgggca aagcctcagg cccgtgggcg atctcggtgt cctcgtctgc ttcgagagcg
      661 tctttcccgg gctcgcgagg acgctcgtgc gatccggcgc gaggctcatc gtgaacatct
      721 ccaacgacgc gtggttcaag acgtggtcca cgccgtggca gcacctccag caggcctgtt
      781 tcaggtcgat cgagacgagg aggtacatgg tccgctcggt gaaccatggg atctcggcgg
      841 tggtggaccc gttcggcagg gtgaagggga gcatcggcct cctcgaggag ggggtgatcg
      901 tggccgaggt cgagatgatc gatttcatga gcctgtacac ccgcttcggc cccctcgtcc
      961 ccttcctgtg ggcggtgtcc tcgaccgcgt gggccttggc gtcgttgaca cgtgcccggc
     1021 caggtggtaa agccaggaac tcatgataac ggacatccag cagcagatgg cgcccctcgg
     1081 tgagcggttg aacgccatga gggggtatct ttgagatccc cgcgcgcgag aagaggctcg
     1141 ccgagatcga cgagctcctg agccggcccg agatctggag cgaccaggca aggacgaggg
     1201 atctcaactc cgagcgctcc aggatctcgc ccatcctcca gcagtggaag accgtcacgg
     1261 gcatctggga agacctcaac gtcctggtcg agctcgccca ggaggacgag cacgcgctgg
     1321 cctcggacat cgagacctcg ctgaaggacc tggagcgcca catagcggag atcgagaaga
     1381 cccacatcct ctccggcagg gacgacccca agtcggcctt cctcgagatc cacgcgggcg
     1441 ccggcgggct cgaggcccag gactgggcgg agatgctcct ccgcatgtac ctgaggtggg
     1501 cggacaagaa cgggtacgcg acccgcatca tcgacatctc ctccgacgag gaggccggga
     1561 tcaagcacgc gacggtggag atatccggcc agtgggcctt cgggctgctc aagggcgaga
     1621 ccggggtgc
//
LOCUS       DAOSCR010000626         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51092_length_1615_cov_3.146154, whole genome shotgun sequence.
ACCESSION   DAOSCR010000626 DAOSCR010000000
VERSION     DAOSCR010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51092_length_1615_cov_3.146154"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLT69_12190"
     CDS             complement(<1..801)
                     /locus_tag="PLT69_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ15245.1"
                     /translation="MENTGKEIVPKVLRQDIGDAELSYLLYDVDGRPMIMLHATGFLP
                     WLWHPIARGLTDTWRVYAPYICDHRVSDPQEGGLSWLTVAEDLVRFVRELGIHRPALV
                     GHSMGATVAAIACGAFGLDALGLVLFEPIFLPEEIYQTPMSVDQHPLASRAIRRKDTW
                     ADEAEARSYLRSKPLFRNWTDEMLDLYVEYGMRRLDAGGLVLACSPVREASIFMGGLR
                     FDPWGVIPKVTCPVLVLEGEKSENRAFIDLQQATRSFPQGTYRLVEGAG"
     gene            complement(1087..>1615)
                     /locus_tag="PLT69_12195"
     CDS             complement(1087..>1615)
                     /locus_tag="PLT69_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15246.1"
                     /translation="LARGDLGRLFSAPASEMRDMLVFSITPKEVTSVQIEYRGESRTF
                     KRMERGPDAKGADKAENAATDHSWEDASGDASSTAVLDSVLATLSKVYCGEYLPDDRK
                     AALGSPVTVITITGRSEARLSIFEREGEQVPAVSSQNPSPFVMPDYKQEELDKALAKI
                     FPGRATAKGAQTPRG"
BASE COUNT          258 a          547 c          515 g          295 t
ORIGIN      
        1 gcccgcgccc tcgacgaggc ggtaggtgcc ctggggaaac gaccgtgtcg cctgttggag
       61 gtcgatgaag gcgcggttct cgctcttttc gccctcgagg acgagcaccg ggcatgtgac
      121 cttggggatg acgccccatg gatcgaacct gaggccgccc atgaagatcg atgcctcccg
      181 cacaggcgag cacgcgagca ccaggccgcc ggcgtcgagc cttcgcatcc cgtactcgac
      241 atagaggtcg agcatctcgt ccgtccagtt cctgaagagg ggtttcgatc tcaggtagga
      301 cctcgcctcg gcttcgtcgg cccaggtgtc tttccgcctt atcgcccgag aggcgagggg
      361 atgctgatcc acgctcatgg gggtctggta gatctcctcg ggcagaaaga tcggttcgaa
      421 gaggaccagc cccagcgcgt ccaacccaaa agcgccgcag gctatggccg ccaccgtcgc
      481 gcccatggag tgcccaacga gtgccggcct gtgtatcccc agctcccgga cgaaccggac
      541 gaggtcctcg gccacggtga gccagctgag gcctccctcc tgcgggtcgg acacacggtg
      601 gtcgcagatg tagggggcat ataccctcca cgtgtccgtc aggccccgtg caatggggtg
      661 ccagagccag gggaggaagc ccgtcgcgtg gagcatgatc atgggccggc cgtcgacatc
      721 gtagaggagg tacgagagct ccgcgtcccc gatatcctgc ctcaacacct tgggtacaat
      781 ctctttgccc gtgttttcca tatacgaacc cctcgtgccc tccgcagcat gcccttagcg
      841 gccccgggtc tcacctcggg ccacggggcc ctcgaacgcc gcttggtgat gtaaacccca
      901 gggaacgccc ctgtctctct gacccgggcc tccatgggca ccgcccggct cccggccgct
      961 gccgcggggt gctgtcgacg cgaagcgccg tccaggagcc ggtatgcccc gctctacgac
     1021 ccgctcgttg tcctggtcga caaggcaccg cacagcatac aaggcgcctg caggttgtcg
     1081 ctgtcgtcat cccctcggtg tctgtgcccc tttcgcggtg gcacgaccgg ggaagatctt
     1141 cgcgagggcc ttgtcgagct cttcctgctt gtagtccggc atgacgaagg gggaggggtt
     1201 ttgcgaggag acggcgggga cctgctcacc ctcccgttcg aagatgctga gcctcgcctc
     1261 gctcctcccc gtaatcgtga tcaccgtcac cggggaacca agagcggcct tccggtcgtc
     1321 gggcaggtac tctccgcagt agaccttgga cagggtggcg aggaccgagt cgagcacggc
     1381 cgtggacgag gcgtcaccgg aagcgtcctc ccaggagtga tcggtcgcgg cgttttcggc
     1441 cttgtctgcc cccttcgcgt cgggtcccct ctccatgcgc ttgaacgtcc tgctttcacc
     1501 ccggtactcg atctgcacgg atgtcacctc tttcggggtg atgctgaaca ccagcatgtc
     1561 ccgcatctcg gaggcagggg ccgaaaacag gcggcccagg tctcccctgg cgagg
//
LOCUS       DAOSCR010000627         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51298_length_1611_cov_2.546915, whole genome shotgun sequence.
ACCESSION   DAOSCR010000627 DAOSCR010000000
VERSION     DAOSCR010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51298_length_1611_cov_2.546915"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(722..>1611)
                     /locus_tag="PLT69_12200"
     CDS             complement(722..>1611)
                     /locus_tag="PLT69_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ15247.1"
                     /translation="THRGLRVHTETGQGTFPVEVDRTQMDHVLLNLFLNAHQAMPKGG
                     DLTVSVDRCELGPQLSALLDLPQGDYVRITVKDTGEGMDEQTMMRIFDPFFTTRAREG
                     GTGLGLASVYGIVKNHGGTVTVTSAPGKGSEFTVYLPVSRKPLVMSTDRDALGARAGT
                     VSGCLLLIDDDEMILEVASEMLTFAGFDVVTAHGGLAGLEYYRAFGDRVDLVILDMVM
                     PDMSGAEVFEKLREMDPTVKVLLSSGYTLNNEAKDIMEKGCNGFIQKPYKIEEITSKI
                     NDIFSRIPGGETPGEHASA"
BASE COUNT          276 a          549 c          494 g          292 t
ORIGIN      
        1 tcgccgagct ctcgggcgaa gaggtcaaag acccgaagaa aaagaagctc ttcggaaggt
       61 tctgacgaaa agccgggcag acctgcaccc tcgcatgccg agcttcaggg aagcggcacg
      121 cttcaggacc tcccccttcc tcaccgggtg cacgaggccc ctctcttcgc agaggcgcac
      181 tgacgcccac ggatggagag gagagatcgg cctcccaaag gccccacccg gcgccgccca
      241 gtcgtgcctc atgcgcgaaa gaccccttgg atgccaaaga agtggcatgg gcatgcttcg
      301 gagtcgttct ttgcgccgag ccttcgtcgt gcaggaaagg cacaaggtgg agagaccgtc
      361 ttcgagttca cgacatgccg cctcagcctg cgggcccgca cccccagggc tccgcgaaga
      421 ggccgcctgg gtggctgccc caagcccttg cccctcttct catgcttccc ccgtacatac
      481 cctcccccgt gagaatgaac gagcgtggcc tcattcctca tgacgatttc cggcctggtt
      541 tccgatgacg atctaacacc gacggccgtc gaggcctgag gcgacatccc acgagcggcc
      601 ctaaggctac gagagtacgg gaaaggtctt gcgcggcctc ggcacgccgg ggtcgacagg
      661 acaggggggt cgggtcaccg aggggccgag cgaagcgcgg gcgccgcatc ggcccggttc
      721 ttcaggcgct tgcgtgctcg cccggcgtct cgccgcccgg gatcctcgag aaaatgtcgt
      781 tgatcttgga cgttatctcc tcgatcttgt agggcttctg gatgaacccg ttgcagccct
      841 tttccatgat gtccttcgcc tcgttgttga gcgtgtaccc gctcgagagg aggaccttca
      901 ccgtcgggtc catctctctg agcttctcga acacctcggc gccgctcatg tcgggcatca
      961 ccatgtccag gatcacgagg tcgaccctgt cgccgaacgc ccggtagtac tccaggccgg
     1021 ccaagccgcc gtgggcggtg accacgtcga accccgcgaa ggtgagcatc tctgaagcca
     1081 cctcgaggat catctcgtcg tcgtcaatga gcaggaggca cccggagacg gttcccgccc
     1141 gggcgccgag cgcgtccctg tcggttgaca tgacgagggg cttcctcgat accggcaggt
     1201 acacggtgaa ctcggacccc ttgccgggcg cgctggtgac cgtcacggta ccgccatggt
     1261 tcttgacgat cccgtacacc gacgccaggc cgagccccgt acccccctcc ctcgccctgg
     1321 tggtgaagaa cgggtcgaag atgcgcatca tggtctgctc gtccatgccc tcgccggtgt
     1381 ctttcaccgt tatcctcacg taatcgccct gcggcaggtc caagagggcc gagagctgcg
     1441 gtccgagctc gcaccggtcg accgagaccg tcaggtcccc ccccttgggc atggcctggt
     1501 gggcgttcag gaacaggttc aacaggacgt gatccatctg cgtcctgtcg acctccacgg
     1561 ggaaggtccc ctgcccggtc tccgtgtgaa ccctgagtcc gcggtgggtt t
//
LOCUS       DAOSCR010000628         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51668_length_1603_cov_3.341731, whole genome shotgun sequence.
ACCESSION   DAOSCR010000628 DAOSCR010000000
VERSION     DAOSCR010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51668_length_1603_cov_3.341731"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="pgeF"
                     /locus_tag="PLT69_12205"
     CDS             <1..581
                     /gene="pgeF"
                     /locus_tag="PLT69_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOJ15248.1"
                     /translation="PDNRIVCLRQVHSDRIVTVDDAPFEGFPEADGVVSADPSTVLCI
                     RTADCVPVLLWDEVLQVSGALHAGWRGLALGIVAKGLDLMRSMGCRDIHVGLGPAIGP
                     CCYTVGPEVVRALGTEPSTSSTGSLSVDLHAVALRQVTAFGVDPSRIHVLRSCTCCTA
                     SYFSYRRDGPAAGRNLSLIGGDACSLPGLPAL"
     gene            551..1168
                     /gene="coaE"
                     /locus_tag="PLT69_12210"
     CDS             551..1168
                     /gene="coaE"
                     /locus_tag="PLT69_12210"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811595.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOJ15249.1"
                     /translation="MFVAGLTGSIATGKSTVSSILEDLGAFIVDADRAAREVVLPGSR
                     AFEEIVSLFGEKILGPDGAIDRARLARIVFRDEGMRRALEALVHPEVMKRMSEQVAGV
                     ASSRPDAVVILDVPLLFETGMHEGLDEVIVVYCPEDEQVRRLVLRDSLSVEEALARVR
                     AQIPIEEKRRIATLVIDNSGPLESTRAQAERVYRMLESRALLRVP"
     gene            1211..>1603
                     /locus_tag="PLT69_12215"
     CDS             1211..>1603
                     /locus_tag="PLT69_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ15250.1"
                     /translation="MAAWYIPTMRHVIEFSTSAREELVDITARVRDLVARHGERCSLC
                     ALFAQGATAALMIQENWDPNIPKDVLGCLRNLVPRGAWLHDEEDGNGDAHIKAGIVGP
                     SEVIPVEDGRLMLGTWQNIIFCEFDGPRR"
BASE COUNT          252 a          524 c          564 g          263 t
ORIGIN      
        1 tccccgacaa ccgcatcgtc tgcctgagac aggtgcactc ggaccgtatc gtgacggtgg
       61 acgacgcacc ctttgagggt ttccccgagg ccgacggcgt ggtcagtgca gacccctcga
      121 ccgtgctgtg catcaggacc gcggactgcg ttcccgtcct cctctgggac gaggtcctcc
      181 aggtatcggg cgccctccat gccggctggc gtggcctcgc cctgggcatc gtcgccaagg
      241 gcctggacct gatgcggtcc atggggtgcc gagacatcca cgtgggtctc ggccccgcca
      301 taggcccatg ctgctacacg gtcggccccg aggtggtgag ggcgctcggg acagagccct
      361 ccacgtcgag cacgggctcc ctctcggtgg acctccacgc cgtggcgctc aggcaggtga
      421 cggccttcgg ggttgacccc tcgcggatac acgtccttcg ctcgtgcacc tgctgcacgg
      481 cgtcgtactt ttcctaccgg agggacggcc cggctgcggg gaggaacctc tcgctgatag
      541 ggggtgacgc atgttcgttg ccgggcttac cggctctata gccaccggca agtccacggt
      601 cagctccatc ctcgaagacc tgggcgcctt catcgtggac gcggaccggg ccgcccggga
      661 ggtggtgctc ccgggcagca gggcgttcga ggagatcgtg tccttgttcg gggaaaagat
      721 cctcggtccc gacggcgcca tagaccgcgc gcggctcgcc cgcatcgtgt tcagggacga
      781 gggcatgcgc agggccctgg aggccctcgt gcacccagag gtcatgaagc ggatgagcga
      841 gcaggtggcg ggcgtcgcct cgtccaggcc cgacgccgtg gtcatcctgg atgtccccct
      901 gctcttcgag acgggcatgc acgagggcct ggacgaggtc atcgtggtgt actgccccga
      961 ggacgagcag gtccgccgcc tcgtgctgag ggactcgttg agcgtggagg aggcccttgc
     1021 gcgcgtgagg gcgcagatcc ccatcgagga gaagaggcgg atcgccacgc tcgtcatcga
     1081 caacagcggg ccgctcgaga gcacccgcgc ccaggcggag agggtgtacc gcatgctcga
     1141 gtcccgggcc ctgctccggg taccctgagt cgcgcgggcg tcgcgcagga agggattcct
     1201 tgaagcaggg atggccgcgt ggtatatccc caccatgcgt cacgtgatag agttctccac
     1261 gagcgcccgt gaggaactcg tggacatcac cgctcgggtg agggatctcg tcgccaggca
     1321 cggcgagcgg tgttcgctgt gcgcgctctt cgcccagggc gcaaccgcgg cgctgatgat
     1381 ccaggaaaac tgggacccga acatcccgaa agacgtgctc ggctgcctga ggaacctcgt
     1441 gccgaggggc gcctggctcc acgacgagga ggacgggaac ggcgacgccc acatcaaggc
     1501 gggcatcgtg gggccttccg aggtcatccc cgtggaagac gggaggctca tgctcgggac
     1561 gtggcagaac atcatcttct gtgagttcga cggaccccgc agg
//
LOCUS       DAOSCR010000629         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51734_length_1602_cov_2.742081, whole genome shotgun sequence.
ACCESSION   DAOSCR010000629 DAOSCR010000000
VERSION     DAOSCR010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51734_length_1602_cov_2.742081"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1602
                     /locus_tag="PLT69_12220"
     CDS             30..>1602
                     /locus_tag="PLT69_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15251.1"
                     /translation="MDITDLKKAEEEARLSSRRYQDILDSMQEAYYEVDLKGNFTFFN
                     FRAIERLGYTLEELNGMNYRRYMDEENARKVYEAFHGVFLTGEPVTGFAWELKDKSGN
                     PIFVEASISLKRDESGSPVGFRGVVRDITEKRKAEDELRASRQRYRDILESMEEGYFE
                     VNLRGEFVSFNEAAMRMTSLSAEELMGMHYHAYAPPETARRMYETFNTVYRTRRPMRM
                     VDYKVIHKDGSLHDNELTVWPKLDKNGEVVGFHTLVRDVTERKKAEDALRVSEERYRA
                     IFENTGSASILIAEDTTVLLCNSTFAKLTGYSKEEIEGKMSWTVFVHEDDLERMKTHH
                     VQRRKDPSLAPSSYEFRLKTRSGEIRDMLLSVVMIPGTKEGVVSCLDITELKRTAEAL
                     KRSEERFRDLARLLPETVFEADKTGRLTFVNDISFATFGYTREDLERGLSLFDMIVPG
                     EREKAAETVARIMAGHHLGLSEYTAMRKDGVTFPVLVHSTPIVQDGRVSGIRGFLIDI
                     TEKKNLESQLMRAQKM"
BASE COUNT          357 a          451 c          535 g          259 t
ORIGIN      
        1 ccggcactgg cgagagcgtg gcctcgtgca tggacatcac ggacctgaaa aaggccgagg
       61 aggaggcgag actgagctcg cggcgttacc aggacatcct cgacagcatg caggaggcgt
      121 actacgaggt cgacctcaag gggaacttca cgttcttcaa cttcagggct atagagcgtc
      181 tgggctatac cctggaagag ctcaacggga tgaactaccg ccgatacatg gacgaggaga
      241 atgcgcgcaa ggtctacgag gccttccacg gggtttttct caccggcgag ccggtgacgg
      301 gattcgcctg ggaactgaag gacaagtcgg gcaatcccat attcgtggag gcgtccatct
      361 cgctgaagag ggacgagagc ggctcccctg tcgggttcag gggcgtggtc agggatatca
      421 ccgagaagag aaaggcggag gatgagctca gggccagccg gcagcgttac cgggacatcc
      481 tcgagagcat ggaggaaggt tacttcgagg tgaacctcag gggggaattc gtgtccttca
      541 acgaggcggc catgaggatg acgagtctca gcgcggagga gctcatgggg atgcattacc
      601 acgcctatgc ccctcccgag acggcgcgcc gcatgtacga gaccttcaac accgtgtaca
      661 ggacccgcag gccgatgcgc atggtggatt acaaggtcat ccacaaggac ggctcgctcc
      721 acgacaacga gctcaccgtg tggcccaagc tggacaagaa cggcgaggtg gtgggtttcc
      781 acaccctggt tagggacgtg accgagcgca agaaggccga ggatgccctg cgggtgagcg
      841 aagagcgtta ccgcgcgatc ttcgagaaca cggggagcgc ttccatcctc atcgcggagg
      901 ataccaccgt gctcttgtgc aattccacct tcgccaagct cacgggttac agcaaggagg
      961 agatcgaggg aaagatgagc tggaccgtct tcgtccacga ggacgacctc gagcggatga
     1021 agacccacca cgtccaacgc aggaaagacc cgtcgctcgc cccgtcgtcc tacgagttca
     1081 ggctcaagac gcgctccggg gagatcaggg acatgctcct ctccgtcgtg atgatccccg
     1141 ggacaaagga gggcgtagtc tcgtgcctgg acatcacgga actcaagagg accgcggagg
     1201 cgctcaagag gagcgaggag cggttcaggg acctggcgag gctgctcccc gagacggtgt
     1261 tcgaggcgga caagacgggg aggctcacct tcgtgaacga catctcgttc gccacgttcg
     1321 gctatacgag ggaggacctc gagcgtgggc tctcgttgtt cgacatgatc gtcccaggcg
     1381 agagggagaa ggcggccgag accgtcgcac ggatcatggc gggccatcac ctcggcctca
     1441 gcgagtacac ggcgatgcga aaggacggcg ttaccttccc cgtcctggtc cattccacgc
     1501 ccatcgtgca ggatgggagg gtctcgggta tcagggggtt cctcatcgac atcaccgaga
     1561 agaagaacct cgagtcgcag ctcatgaggg cccagaagat gg
//
LOCUS       DAOSCR010000630         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51782_length_1601_cov_3.091850, whole genome shotgun sequence.
ACCESSION   DAOSCR010000630 DAOSCR010000000
VERSION     DAOSCR010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51782_length_1601_cov_3.091850"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLT69_12225"
     CDS             complement(<1..350)
                     /locus_tag="PLT69_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOJ15252.1"
                     /translation="MNVINRDRLFEMVSVGTPVEGEYLRGLDLSSLDLGCANLRGSDL
                     GKVNLTDVNLEESHLERANLDEAVFKNTNLSGAHLSETSLKDTDLSSCTLTRITLVNT
                     RLTRVTIAGSDMRKA"
     gene            complement(347..>1601)
                     /locus_tag="PLT69_12230"
     CDS             complement(347..>1601)
                     /locus_tag="PLT69_12230"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOJ15253.1"
                     /translation="AREKIGNQWVVFARGVKPEAGSDGYVKFHFEKERPRVVIKEDPT
                     GKVNLRDLNLIQNVKKGDILCEVIQPRPGKNGITVRNEEIPGKEGTPAKLPGGTNVSY
                     SADRTQMFASLDGLVRWAENMVIVEPVYTVHDVDASVGNIRFNGSVVVQGEVGDGYEI
                     HAGKDITIASTVGRVILEAGGDIRIAGGILGQEKALVTANGGIHVKFVQDAHVRAEKE
                     IVVSDYIRNSLVSAAGPLVVKNPKGFIVSSKVSSDTWIYCNTVGAEDAGIPTELSIGH
                     SPVLRQEEERLKAEIEEKALDFLRLHSSLVKLRAIRSSSELTRQQEMLYERILEAMES
                     LRKTLLGKNARLIEIANKMSRAFLGNIYIEGRAYPGTTIRIGMASITLSEPADNIHYS
                     IHDGQVVSHGFICVPEVKKILEAKE"
BASE COUNT          231 a          517 c          485 g          368 t
ORIGIN      
        1 gccttcctca tgtcacttcc tgcgatggtc acccgggtga gcctggtgtt cacgagggtg
       61 atcctggtca gggtgcagct gctcagatcc gtgtccttga gggaggtctc gctcaggtgg
      121 gcgcccgaga ggttggtgtt cttgaacacg gcctcgtcca ggttcgcgcg ttccaggtgc
      181 gattcctcga gattgacgtc cgtgaggttg accttgccga ggtccgatcc cctgaggttg
      241 gcgcacccga ggtcgaggga cgagaggtcg agacccctga ggtactctcc ctcgaccggc
      301 gtgccgacgg agaccatctc gaagagcctg tcgcggttga tgacgttcat tccttggcct
      361 ccaggatctt cttcacctcg gggacgcata tgaacccgtg cgagaccacc tggccgtcat
      421 gtatggagta atggatgttg tccgccggct cggagagcgt gatgctcgcc atgcctatgc
      481 gtatcgtggt ccccgggtat gccctgccct cgatgtagat gtttcccaag aaggcgcggg
      541 acatcttgtt cgcgatctcg atgagccgtg cattcttgcc gaggagggtc ttcctgaggc
      601 tctccatggc ctcgaggatc ctctcgtaga gcatttcctg ctggcgcgtg agctcgcttg
      661 acgacctgat cgccctgagc ttcacgagcg acgagtgcag cctcaaaaag tcgagggcct
      721 tctcctctat ctccgccttg agcctctcct cctcctgcct gagcaccggg ctgtggccga
      781 tgctgagctc ggtggggatc ccggcgtcct ccgcccccac ggtgttgcag tagatccacg
      841 tgtccgagga caccttgctg gagacgatga accccttcgg gttcttgacc accaagggcc
      901 ctgcggcgga gacgagactg ttgcggatgt agtcgctcac cacgatctct ttttccgccc
      961 tcacgtgggc gtcctggacg aatttgacgt gtatgccccc gttggccgtc accagggcct
     1021 tctcctggcc gaggatgcct cccgctatcc tgatgtcgcc ccccgcctcc aggatgaccc
     1081 tccccaccgt ggaggcgatg gtgatgtctt tgcctgcgtg gatctcgtac ccgtcgccca
     1141 cctcgccctg gacgacgacc gagccgttga accggatgtt gcccacggat gcgtcgacgt
     1201 cgtggaccgt gtagacgggt tccacgatga ccatgttctc ggcccacctg acgagcccgt
     1261 ccagggatgc gaacatctgg gtcctgtccg ccgagtagct cacgttcgtg ccgcccggca
     1321 gttttgccgg ggttccttcc ttccccggta tttcctcgtt cctgacggtg atgccgttct
     1381 tgcccggacg gggctggatc acctcgcaga ggatgtcgcc cttcttcacg ttctggatga
     1441 ggttcaggtc gcggaggttg accttgccgg tggggtcttc cttgatgacc acccggggcc
     1501 gctccttctc gaagtggaac ttcacgtagc cgtcggagcc ggcttccggc ttgacacccc
     1561 tggcgaagac cacccactgg ttcccgatct tctcccgggc c
//
LOCUS       DAOSCR010000631         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51927_length_1598_cov_2.778354, whole genome shotgun sequence.
ACCESSION   DAOSCR010000631 DAOSCR010000000
VERSION     DAOSCR010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51927_length_1598_cov_2.778354"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLT69_12235"
     CDS             complement(<1..813)
                     /locus_tag="PLT69_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOJ15254.1"
                     /translation="MDKFADKPIALPEGIDMEKHVVATYWVQSDRTMDMALMGQILAI
                     EQTTGTWVPVPGETPEVRAKHVAKVIGVYEAPFYEYGLPEDVKARQYIVQIAFPAANV
                     EDQLPMLLTATIGNISLVPNFKLLDLRMPRETLEGYQGPAFGIDGWYEALGIEKNRPL
                     LDNKIEACSGYALEVGCKLFREVAMGGCDVVKDDELIANMKYNDAVERVKKYMEIERQ
                     VYEETGEHTLYTVNVTDRLPRMFDLAKRCIDAGANAMMVNYLAVGPEAMRALV"
     gene            complement(847..>1598)
                     /locus_tag="PLT69_12240"
     CDS             complement(847..>1598)
                     /locus_tag="PLT69_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ15255.1"
                     /translation="KAFEDNKDRGGVAGILVEPIGGESGTHPVHPDWNRRIRELCDHY
                     GALLIFDEVVTGFRLGMGGAQKLLGITPDLTVLGKIIGHGYPSAGAVGGRKDVMSVCQ
                     GGLGEKIYIGGTLAANPITTAACYYALRLMEEHDAPGKAAAYADRLTKAMNDLFSTRP
                     DLGFFVYNFGPIMHYVTTSFFSIDLSQPDAFTQIMSRKQAADDYQMVTTAHGLCTLAG
                     TRMYTCMQHDDEALAKTIETWEYLLSLIPKA"
BASE COUNT          237 a          516 c          509 g          336 t
ORIGIN      
        1 aaccagggcg cgcatggcct cgggacccac ggcgaggtag ttcaccatca tggcgttggc
       61 ccccgcatcg atgcaccgct ttgccaggtc gaacatgcgg ggcagccggt cggtcacgtt
      121 caccgtgtag agcgtatgct cgcccgtttc ctcgtatacc tgccgctcga tctccatgta
      181 cttcttcacg cgctccaccg cgtcgttgta tttcatgttg gcgatcagct cgtcgtcctt
      241 gacgacgtcg cagccgccca tggccacctc gcggaagagc ttgcagccca cctcgagagc
      301 gtatccgctg caggcctcga tcttgttgtc gagcaggggc cggttcttct cgatccccag
      361 cgcctcgtac cagccgtcga tgccgaaggc aggcccctgg tagccttcca gggtctccct
      421 gggcatcctc aggtcgagga gcttgaagtt gggcaccagc gagatgttgc cgatggtggc
      481 cgtgagcagc atgggcagct ggtcctcgac gttggcggca gggaaggcga tctgcacgat
      541 gtactgcctg gccttgacgt cctcgggcag gccgtactcg tagaagggcg cctcgtagac
      601 gccgatcacc ttggccacgt gcttggccct gacctcgggc gtctccccgg gcaccggcac
      661 ccaggtgccg gtggtctgct cgatggccag gatctggccc atgagcgcca tgtccatggt
      721 ccggtctgac tggacccagt aggtagccac cacgtgcttc tccatgtcga tgccttccgg
      781 cagggcaatg ggcttgtcgg cgaatttgtc catggtttct cctttatcgg gctcggcttt
      841 tcgtcactat gccttcggga tgagcgaaag caggtactcc caggtttcga tcgtcttcgc
      901 gagggcctca tcgtcgtgct gcatgcaggt gtacatgcgg gtgcctgcga gcgtgcacag
      961 gccgtgggcc gtggtgacca tctggtagtc gtccgccgcc tgcttgcggc tcatgatctg
     1021 ggtgaaggcg tcgggctggg agaggtcgat ggagaagaac gacgtggtca cgtagtgcat
     1081 gatgggcccg aagttgtaca cgaagaaccc caggtcgggc cgcgtggaaa acaggtcgtt
     1141 catggccttc gtgagccggt cggcgtaggc ggccgccttt cccggggcgt cgtgctcctc
     1201 catgagcctg agcgcgtaat agcaggcagc cgtggtgatc gggttggccg cgagggttcc
     1261 cccgatgtag atcttttcgc ccaggccgcc ctggcacacg ctcatcacgt cctttctccc
     1321 gcccacggct ccggcggacg ggtagccgtg cccgatgatc ttgcccagca cggtcaggtc
     1381 gggcgtgatg cccaggagct tctgtgcacc ccccatgccg agcctgaacc cggtcaccac
     1441 ctcgtcgaag atcaacagcg caccgtagtg gtcgcagagc tcccgtatcc tgcggttcca
     1501 gtccgggtgg accgggtggg tccccgactc tccgccgatg ggctccacca ggatgcccgc
     1561 gactccgccc ctgtccttgt tgtcttcgaa ggccttct
//
LOCUS       DAOSCR010000632         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_51934_length_1598_cov_2.549579, whole genome shotgun sequence.
ACCESSION   DAOSCR010000632 DAOSCR010000000
VERSION     DAOSCR010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51934_length_1598_cov_2.549579"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLT69_12245"
     CDS             <1..500
                     /locus_tag="PLT69_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15256.1"
                     /translation="LADLAVDAVYDAGGGLWHELEVEANVNPARPLSLKHLNLLQRSR
                     GRDLATVQHSTSLVVSSRRGDSFSFGYEYLRDGSKLLRAQADALIAGGLAAGIEARYD
                     HEKGKFEEHAAELRYTSQCWAVSLTRRVDASDDLERSRSTWSVNVKLLGVGDVPVTSP
                     PDREG"
     gene            504..1061
                     /locus_tag="PLT69_12250"
     CDS             504..1061
                     /locus_tag="PLT69_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ15257.1"
                     /translation="MIPDKDAIRDLLEKQKVPSHILEHQEVVCRVALALARALRKAGY
                     PVDLGLVEASALLHDICKMDGIATGQDHAGMAEEVLVRAGYPEVACVVGQHVRLRSFR
                     LDEAMVVNYADKRVMHAQVVTLEERFKDLMERYGTDEARRARIREHASACEEMERIIA
                     GAIGAGVQELEGLCLVPLDETVDGL"
     gene            complement(1020..>1598)
                     /locus_tag="PLT69_12255"
     CDS             complement(1020..>1598)
                     /locus_tag="PLT69_12255"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOJ15258.1"
                     /translation="KAWVKQFLDAQVGAIEGEEALFTILSLSTGVFKFVGGMKSPKTS
                     ISTNWEYLLIEGMRRADEMSLALERGDIHDADVAPVDESTRMLVKNLSSLSECAGIAV
                     VRSEDDVLYQSGTITKEVDVPAVAGFFVNLPDILGELCTRRPRRVSYIDQGRLVIVCP
                     FKIFVVVMLFKKSALPPDVLQTIDRLIQRYEA"
BASE COUNT          292 a          492 c          553 g          261 t
ORIGIN      
        1 ccctggccga cctcgctgtc gacgcggtct acgacgcggg cggcggcctg tggcacgaac
       61 tcgaggtgga agcgaacgtg aacccggcga gacccctctc gctgaagcac ctgaacctgc
      121 tgcagcgctc ccgcgggcgg gatctcgcca ccgtgcagca ctcgacctct ctcgtggtgt
      181 cgtcgaggag gggagactcg ttctccttcg gctacgaata cctcagggac ggatcgaagc
      241 tccttcgcgc ccaggcggat gccctgatcg cagggggcct tgccgccggc atcgaggcca
      301 ggtacgacca cgagaagggg aaattcgagg agcacgcggc ggagctcagg tacacgtccc
      361 agtgctgggc ggtctccctg acgcggaggg tggacgcatc ggacgacctc gagagatcga
      421 ggagcacgtg gtcggtcaac gtgaagctgt tgggggtggg cgatgtgccc gtgacgagcc
      481 ccccggaccg cgaggggtga cgaatgatcc cggacaagga cgccatcagg gacctgttgg
      541 agaaacagaa ggtgccgagt cacatacttg agcaccagga ggtggtgtgc agggtcgccc
      601 tcgcactggc gagggcgttg cgcaaggccg ggtacccggt cgacctcggc ctcgtggagg
      661 cctccgccct cctccacgac atctgcaaga tggacggcat cgccacgggg caagaccatg
      721 cgggaatggc cgaggaggtg ctcgtgagag cggggtaccc ggaggtcgcc tgtgtggtgg
      781 gccagcacgt gaggctccgc tcgttcaggc tcgacgaggc gatggtggtc aactacgcag
      841 acaagagggt catgcacgcg caggtggtca cgctcgaaga gcgcttcaag gatctcatgg
      901 agcggtacgg gaccgacgag gcgaggcggg ccaggatccg cgagcatgca tccgcctgcg
      961 aggagatgga gaggatcatt gccggtgcga tcggcgcggg cgtgcaggag ctcgaggggc
     1021 tatgcctcgt accgctggat gagacggtcg atggtctgta ggacgtccgg cgggagggcc
     1081 gacttcttga acagcatcac gaccacgaag atcttgaacg ggcacacgat cacgagcctt
     1141 ccctggtcta tgtaggacac ccgcctcggc ctgcgggtgc acagctcgcc caggatgtcc
     1201 ggcaggttga cgaagaatcc cgccacggcc gggacgtcga cctccttcgt tatggtgccg
     1261 ctctggtaga ggacgtcgtc ttccgacctg accacggcga tcccggcgca ctcgctcaag
     1321 gagctgaggt tcttcacgag catgcgggtg gactcgtcca cgggagccac gtcggcgtcg
     1381 tggatgtccc cgcgttccag cgcgaggctc atctcgtcgg cacgacgcat cccctctatg
     1441 aggaggtact cccagttcgt agagatgctc gttttcggcg acttcatccc gcccacgaac
     1501 ttgaacacgc ccgtggacaa tgacaggatg gtgaacaacg cctcctcgcc ctcgatggcg
     1561 cccacctgcg cgtcgaggaa ctgcttgacc cacgcctt
//
LOCUS       DAOSCR010000633         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_52219_length_1592_cov_3.811971, whole genome shotgun sequence.
ACCESSION   DAOSCR010000633 DAOSCR010000000
VERSION     DAOSCR010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52219_length_1592_cov_3.811971"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLT69_12260"
     CDS             <1..194
                     /locus_tag="PLT69_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005391924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15259.1"
                     /translation="ARLFAEQLEARGVRVTPGDIYGPGWTQHVRISLLGPRPEELKRG
                     LEIVRDHLGRARQPGLTEF"
     gene            514..1227
                     /gene="sfsA"
                     /locus_tag="PLT69_12265"
     CDS             514..1227
                     /gene="sfsA"
                     /locus_tag="PLT69_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOJ15260.1"
                     /translation="MQEPLYPWPELTRGILIERQKTFTARVLLDDGSLVRAHCPNTGR
                     MLTCSEPLRPVYLAHSTNPGRSTSHTWEMIEMPGSLVVVDTMVANRLALAAVCRGRVP
                     ELACYETARPEVAFHGRSRFDLLLEAPGKPGCIVEVKSSTLVEDGVAMFPDAVSKRAL
                     AHVEELGRMGRLGFRTVMLFLIMRNDALAFRPASHVDPAFCRALSAALGDGLEVMAYR
                     ADVGLAGVSMGHRVPVQPW"
BASE COUNT          274 a          543 c          536 g          239 t
ORIGIN      
        1 ccgcacgcct gttcgccgaa cagctcgagg cgcgcggggt gagggtcacg ccgggagaca
       61 tctacggtcc agggtggacc cagcatgtcc gcatatcgct cctcgggccc cgccccgagg
      121 agctcaagag agggctcgag atcgtgcgcg accacctcgg ccgcgcacgc cagccgggcc
      181 tcaccgagtt ctgagctcga ggccggcttc aggcgccgtc ctcgagacgc tcctccgcac
      241 ctcgactcct cccgcccgcc aaggccaacc ggccttggga gttcttccgt ccgtttcccg
      301 gggacacggc ccgaggccgc ttgcctttgg gaccgccgaa ggccgcgtgc tcctttccag
      361 gtctcggcga catccctggg caggggcatc cccgagcgtg aaccgggggc attcccggga
      421 gcgcaggtca cgcctgccgc gacgagagga tcggtgaggc gcctccagcc gcgaaggtgg
      481 cgcaaggcgc ccgctgcatg gtaagggacg accatgcagg agccgttgta cccctggccg
      541 gaactcacca gggggatcct catcgagaga cagaagacct tcacggcacg tgtcctgctg
      601 gacgacggca gcctcgtaag ggcgcactgc ccgaatacgg gcaggatgct cacgtgctcg
      661 gagcctctca ggccggtgta cctcgcccac tcgaccaacc cgggccgctc cacgagccat
      721 acctgggaga tgatcgagat gccgggctcc ctcgtggtgg tcgacaccat ggtcgccaac
      781 aggctcgcct tggccgccgt ctgcaggggg cgggtccccg agctcgcctg ctacgagacg
      841 gcacggccgg aggtcgcttt tcacggccgt tcgcggttcg acctcctgct ggaagcgccg
      901 ggcaagcccg gctgcatcgt ggaagtcaag agttcgacgc tcgtggagga cggggtcgcc
      961 atgttcccgg acgccgtctc caagcgcgcc ttggcccacg tggaggagct gggacggatg
     1021 ggacgcctgg ggttcaggac agtgatgctg ttcttgatca tgcgcaacga cgccctcgcc
     1081 ttcaggcctg cgtcccacgt cgaccccgcc ttctgcaggg cgctctctgc cgccctgggc
     1141 gacggcctcg aggtcatggc ctaccgtgca gacgtcggtc ttgcaggcgt atccatgggc
     1201 cacagggtgc ctgtccagcc gtggtgagca ggcatcgatc ctgcatgaaa cccgtcggcc
     1261 gggaaagggg cgcaagcgac gcccgccccg tcgcacccgg gcttcccgcc atggagtgcg
     1321 tcacccggtg gcgacgaccg ggtggaagac gccccggccg gaatccgtgt aaaggaaccg
     1381 aatggcttga aaaaaggccg gctgcatcct aatgagagag aggaaggtga acggccatga
     1441 agaagcccgc atggacaccg agcaaggacg aagccctggc cggcggcgag ggtgcccgcc
     1501 ggaaagaccc ggctcagcgt cttgcccgag taaaggacgc cccaaggaat ttcttcctgg
     1561 agatcgcgca ctccctcatg gacaagaaca gg
//
LOCUS       DAOSCR010000634         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_52434_length_1588_cov_2.519896, whole genome shotgun sequence.
ACCESSION   DAOSCR010000634 DAOSCR010000000
VERSION     DAOSCR010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52434_length_1588_cov_2.519896"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..773)
                     /locus_tag="PLT69_12270"
     CDS             complement(399..773)
                     /locus_tag="PLT69_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOJ15261.1"
                     /translation="MKVSDREFARIVARALDNIPEEIMRHVENVAVTIEDRHSPEVLE
                     EMGLEPDEPLFGLYTGVALTEREISSPELMPDTIVIFKEPLLEYCTSREELEEEIEIT
                     VVHEIAHYFGISEERLAELGYD"
     gene            complement(826..1326)
                     /locus_tag="PLT69_12275"
     CDS             complement(826..1326)
                     /locus_tag="PLT69_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOJ15262.1"
                     /translation="MAAPQDITPAGQSAFFKDPSAPDNEHETDKLTRNVILSAYRYLG
                     SPYRYGGTSPSGFDCSGFVRYVFEENGISLSRSSRTQVHEGMPVDLSSLKPGDLVFFK
                     MRRGVRAAVDHVGIYVGEGFFIHASSRGAKEISLDSLESSPYRDTMVGARRILESPSD
                     ESFVVE"
BASE COUNT          253 a          481 c          559 g          295 t
ORIGIN      
        1 tcagggagag tctcctggag acgaagcgtg acctcgtcgt gaaggaatac ctcgcccagg
       61 ccaaggacga caagcgcggc tggcacaagg tgaggatcga gagggacaag atcgcgtcgc
      121 acggcgggaa ggggcagcgc ggggatacgc cgccgcccga ggggctgtac tgacgggggg
      181 gcaggcgcgg cggcggcccc gccgcgcgca ccccgctcga ggcagaggct ccccgaggtg
      241 gtttgccctg agctgccgtc gaggaccagc caacctgaaa agcaccgggg gtcgcgcacc
      301 ccgggctggg gaacattgcc ggatcaggca accgggcggc tgagggcaag cccggtctcg
      361 gcgtgttgtg ctgacgcagg tcgtgaaggt acgccggttc agtcgtagcc gagttccgcc
      421 agccgctcct cgctgatgcc gaagtagtgc gcgatctcgt gcacgaccgt gatctctatc
      481 tcctcttcga gttcttccct gctcgtgcag tactcgagga ggggctcctt gaatatcacg
      541 atcgtgtccg gcatgagctc gggggacgag atctccctct cggtcagggc cacgcccgtg
      601 tagaggccga agagcggctc gtcaggttcc aggcccatct cctcgagcac ctcgggggag
      661 tggcggtcct cgatggtgac ggccacgttt tccacgtggc gcatgatctc ctcggggatg
      721 ttgtcgagcg ccctggccac gatccttgcg aactctctgt cggaaacctt catggtcctt
      781 gcgtacggtg tacggcagtg ctggtcatgt ggcaaggcgt gcccgtcatt cgacgacgaa
      841 ggactcgtcg ctggggctct cgaggatcct tcgcgccccc accatggtgt cgcggtaggg
      901 gctcgactcg agcgagtcca gcgagatctc cttggctccg cgggaagagg cgtggatgaa
      961 gaacccttct cccacgtaga tccccacgtg gtcgaccgcg gccctgacac cccgtcgcat
     1021 cttgaagaag acgaggtcgc ccggtttcaa cgaggagagg tcgacaggca tcccctcgtg
     1081 gacctgggtg cgtgagctgc gggacaggga gatgccgttc tcctcgaaga cgtagcgcac
     1141 gaagcccgag cagtcgaacc ccgagggcga ggtcccccca tagcggtagg gggagccaag
     1201 gtagcggtac gcggacagga tgacgttgcg ggtgagcttg tccgtctcgt gctcgttgtc
     1261 gggcgcggac ggatccttga agaacgctga ctgccccgcc ggcgtgatgt cctgaggggc
     1321 ggccacgaga gctggttttt cgtgaggcga gacatgagcg gcggtgtgcc tggcttggct
     1381 cgagtgacgg ccgcctttgc ccgcgtgtcg cgacgtcacc ctcgtgcggc ttgtgtgttt
     1441 cttgcccgaa gagagggcct cgcccttctt tttcttggga gaaagggcgg cctttgcgtg
     1501 gtgatccttc cgggcatggg cacggccggc cgtccggttc gttgcgctac ccgtcttatc
     1561 gcgccccagg gaagatgcgc ctttttgg
//
LOCUS       DAOSCR010000635         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_52483_length_1587_cov_3.334204, whole genome shotgun sequence.
ACCESSION   DAOSCR010000635 DAOSCR010000000
VERSION     DAOSCR010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52483_length_1587_cov_3.334204"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /gene="rplI"
                     /locus_tag="PLT69_12280"
     CDS             <1..474
                     /gene="rplI"
                     /locus_tag="PLT69_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ15263.1"
                     /translation="PKRRIYMNVVLLETIEGLGSVGQEVKVKDGYARNYLIPKGLALL
                     ATDKNIKAFKSKIEARIRSEAKSREHAQKLSEQLASVTLRFKAKTGQEGKLFGSITTS
                     DIFEQLEAKGFEVDKKKIHLAEPIRHTGTHEVDVRLFPGVVASVKVEVVPEDAAD"
     gene            476..>1587
                     /gene="dnaB"
                     /locus_tag="PLT69_12285"
     CDS             476..>1587
                     /gene="dnaB"
                     /locus_tag="PLT69_12285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOJ15264.1"
                     /translation="MDDKSLSLPYSREAEEYILGGILMENTALDSVCTIVSAEDFYIE
                     RNRILFEQMVRLMDKGLPIDLVSLTESVERTGKLDKVGGAAYISQLADIVPTTANIEY
                     YARIVRDKSILRSLINGASEIIRQARSQAGEVADILESAEQIVYAINREIEQSGRGLT
                     KIGQPIQNLYEKLRRMASGEVDPGIVSTGFERLDIQLLGGLHRSDLIIIAGRPSMGKT
                     SLALNIAQNVAVRERLPVAVFSLEMGIDQLALRMLAAEAWVDQGLVRSSMSVQRLRKE
                     DWSKLIEASEKLYEAPIYIDDTPGVSPMEIRSKVRRLMSQVDLRLIVVDYLQLMSLKA
                     RTDSREQEISEISRTLKAIAKEFNVPLIAVSQLNRRV"
BASE COUNT          345 a          477 c          507 g          258 t
ORIGIN      
        1 ccgaaaagga gaatatacat gaacgttgtg cttcttgaga ccatcgaggg cctggggagc
       61 gtcggccagg aagtgaaggt gaaggacggc tacgcgagga actacctcat cccgaagggt
      121 ctcgcgctgc tcgccaccga caagaacatc aaggccttca agagcaagat cgaggcacgc
      181 atcaggtccg aggccaagtc gcgcgagcac gcccagaaac tgtccgagca gctcgcatcc
      241 gtcaccctcc ggttcaaggc gaagaccggt caggaaggca agctcttcgg ctccataacc
      301 acctcggaca tcttcgagca gctggaagcc aagggtttcg aggtggacaa gaagaagatc
      361 cacctcgcgg agcccataag gcacacggga acccacgagg tcgacgtgcg gctcttcccg
      421 ggtgtcgtgg cgagcgtgaa ggtcgaggtg gtgcctgaag acgcggccga ttgagatgga
      481 cgacaagtct ctctccctcc cgtacagcag ggaggcggag gagtacatcc tcggcgggat
      541 actcatggag aacaccgccc tcgattcggt gtgcaccatc gtgtccgccg aggatttcta
      601 catcgagcgc aacaggatct tgttcgagca gatggtgcgc ctcatggaca aggggctgcc
      661 catcgatctg gtgtccctta ccgagtcggt cgagaggacg gggaagctcg acaaggtggg
      721 cggcgcggcc tacatatcgc agcttgcgga catcgtcccc acgaccgcga acatcgagta
      781 ctacgcacgg atcgtcaggg acaagtccat cctcaggtcc ctgatcaacg gcgcctcgga
      841 gatcatccgg caggcgaggt cccaggcggg cgaggtcgcg gacatcctcg agtcggccga
      901 gcagatcgtc tacgccatca acagggagat cgagcagtcc ggccgcggcc tcaccaagat
      961 agggcagccc atccagaacc tctacgaaaa gctcaggcgc atggccagcg gcgaggtgga
     1021 cccgggcatc gtgtcgaccg gtttcgagcg cttggacatc cagctcttgg gtgggttgca
     1081 caggtccgac ctcatcatca tcgcaggcag gccttccatg gggaagacct cgctcgcctt
     1141 gaacatcgcc cagaacgtgg ccgtcaggga gaggctgccg gtggccgtct tctccctcga
     1201 gatggggatc gaccagctcg ccctgcgcat gctcgcggcg gaggcctggg tggaccaggg
     1261 gctcgtgagg tcgtccatga gcgtgcagcg gctcaggaag gaagactgga gcaagctcat
     1321 cgaggcttcc gaaaagctct acgaggcgcc catctacatc gacgacacgc ccggggtctc
     1381 gcccatggag atcaggtcca aggtgcgtag gctcatgagc caggtcgacc tgcgactcat
     1441 cgtggtcgat tacctccagc tcatgtctct caaggcgaga acggactcga gggagcagga
     1501 gatctcggag atctcgcgca ccctcaaggc catagccaag gagttcaacg tcccgctcat
     1561 agcggtgagc cagctgaaca ggcgcgt
//
LOCUS       DAOSCR010000636         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_52823_length_1580_cov_2.550164, whole genome shotgun sequence.
ACCESSION   DAOSCR010000636 DAOSCR010000000
VERSION     DAOSCR010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52823_length_1580_cov_2.550164"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLT69_12290"
     CDS             complement(<1..836)
                     /locus_tag="PLT69_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HOJ15265.1"
                     /translation="MNPHVFREYDIRGVVGQDLTFEFVERLASALGVYYSERGASRVS
                     LGMDARLSSPDFKEILKQGLVSSGIDVVDLGMVPTPVMYFSLFKTDVHGGIEVTGSHN
                     PPDNNGFKIALGKTTIYGEEILKLRRLMEEGRRVGGRGRSRSYDIVPEYVDDIVSRIR
                     PGSRRLRVVIDPGNGVGGAPAMAVCEALGVEAFGICLEPDGRFPNHHPDPTTEEGIAM
                     LRDEVIRLKADLGIGYDGDADRIGVVDARGRTIYGDMITTVLAGEILRERPGAKIIGE
                     VK"
BASE COUNT          256 a          579 c          472 g          273 t
ORIGIN      
        1 cacttcacct ccccgatgat cttggcgccg ggccgttccc tcaggatctc gccggcgagg
       61 acggtggtga tcatgtcgcc gtagatggtc ctcccgcggg cgtccacgac gcctatgcgg
      121 tcggcgtcgc cgtcgtagcc gatgccgagg tccgccttca gcctgatcac ctcgtctctc
      181 agcatggcga tgccttcttc cgtggtcggg tcgggatggt ggttggggaa gcgcccgtcg
      241 ggctcgaggc agatgccgaa ggcctccacg ccgagagcct cgcacaccgc catggcggga
      301 gcccccccga cgccgttccc agggtcgatc accaccctga gccttctgga tccaggccgg
      361 atcctggaga cgatgtcgtc cacgtactcg ggcacgatgt cgtacgagcg ggaccggccc
      421 ctgccgccca cccggcgccc ctcctccatg aggcgcctga gcttcaagat ctcctccccg
      481 tagatcgtcg tcttcccgag ggcgatcttg aacccgttgt tgtccggcgg gttgtgggaa
      541 cccgtcacct cgatgccgcc gtgcacgtcc gtcttgaaga gcgagaagta catgacaggg
      601 gtggggacca tgccgaggtc caccacgtcg atgccgctcg agacgagccc ctgcttgagg
      661 atttccttga agtccggcga gctgagcctg gcgtccatgc cgaggctcac ccgtgatgcg
      721 ccgcgttcgc tgtagtagac gcccagcgcc gaagcgagcc tctccacgaa ctcgaaggtc
      781 aggtcctgac ccaccacccc ccgtatgtcg tactcgcgaa acacgtgagg attcatactc
      841 cccccattca taccgtctcg attggcgacc cagcagcccc cgaaggggac cgtccgcgtt
      901 cacggaccgg gccccgcccg tacctcaaca aaaccgaggg atccctgccc gcctctcccg
      961 cccggccgca ggcccaccat gcccagaagc gtcgatggct ccgtcccggc ccttgaggaa
     1021 gtgcattgcc tccgtcatgc gacgcccagc ccgtggaatg gagtaccccg cccctttgcc
     1081 ggcctgagag gcccttctct ttcacccgat catacccaag cgcccggagc gtggtcaagg
     1141 aacatggggt cttcccgctc aagggaaaga gcacctttct cgagggtatc aacggccatc
     1201 gccctgttgt caagggacca ggccgttgag ttgcgctctc tggctcgaca tcccctctcg
     1261 tgacccggct gtcccccaaa cccttgggcc caatcaaacc acgaccttga cgaggaaagg
     1321 gatgaagagg aatgagaggg ggacgtggcg cccgcccggc cctccagccc acggccgcct
     1381 gcgcccctgg ggtcttctgg tgaagccaca cgcaacccgg ccctccctcc caatgccgtc
     1441 tgggtttccc gcccgtcccg tcggtctcga cgacttcgag cgcgcccgtc ggctgcacgt
     1501 tcggcgagaa cgcgacgccc cactccggac tcgtggacgg ccgggccggg gcgacggcac
     1561 tcggagcatc caggattgac
//
LOCUS       DAOSCR010000637         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53081_length_1574_cov_2.815010, whole genome shotgun sequence.
ACCESSION   DAOSCR010000637 DAOSCR010000000
VERSION     DAOSCR010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53081_length_1574_cov_2.815010"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLT69_12295"
     CDS             <1..262
                     /locus_tag="PLT69_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ15266.1"
                     /translation="LRPAFIVFTDISRDGMLTGPDIEATVSLARSVSTPVIASGGVSS
                     LEDIRALAATGVVAGVITGKAIYEGRFDVAQAVRASRSRAGA"
     gene            360..695
                     /locus_tag="PLT69_12300"
     CDS             360..695
                     /locus_tag="PLT69_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOJ15267.1"
                     /translation="MDCIFCRIVSGEVPSTKVYEDELVYAFEDIQPLAPVHVIVVPKR
                     HVATLNELDDSDVWFSMLKAAKETAKIKGVAETGYRIAVNCGRDGTQIVMHLHLHVLG
                     GRLLDGKLG"
     gene            complement(798..>1574)
                     /locus_tag="PLT69_12305"
     CDS             complement(798..>1574)
                     /locus_tag="PLT69_12305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ15268.1"
                     /translation="LHRGLLGELLQRRAAREAVLQVGQHLGDVAAGLGCRGLRPEVGP
                     LKGGGLGGDVVVSENGLSYAVDVLTGDRTGFYLEHRLTRSRIAAYAKGRRVLDLFSYS
                     GSFSVCALAAGAVSVASVDSSAPAQALLRKNMELNRIRPFVWRHHRDDAVSFLEADRD
                     SYDLVLVDAPASVSEYREYRRVLDRAVARLGSPGILFVAAGFNAQFSGPDLLKAVHKS
                     ASGLSRNVRVLETLSGPADFPWLPSHPEGLFQGGFVLYLD"
BASE COUNT          283 a          516 c          558 g          217 t
ORIGIN      
        1 gcttcggccg gccttcatcg tgttcacgga catctcgagg gacgggatgc tcaccgggcc
       61 ggatatcgag gccaccgtct cgctggcgcg ctcggtgtcg accccggtga tagcgtcggg
      121 aggggtgagc agcctcgagg acatccgggc tcttgccgcc accggggtcg tggcgggggt
      181 catcacggga aaggcgatct acgaaggccg cttcgacgtg gcacaggcgg tgcgagcctc
      241 gagatcccgg gcgggcgcgt gagccggcgg tcccggccgc cggtgtcccg aggaaagaac
      301 cggggcgaag cgagcaagac gacgccctgc aaggatatgc gaatgtacgg gaggtaggca
      361 tggactgtat cttctgcagg atagtctcgg gcgaggtccc ctccaccaag gtgtacgagg
      421 acgagctcgt ctacgccttc gaggacatcc aacccctggc gccggtgcac gtgatcgtgg
      481 tgcccaagcg tcacgtggcc accctgaacg aactcgacga cagcgacgtc tggttctcca
      541 tgctgaaggc ggccaaggag acggcgaaga tcaagggcgt cgccgagacc ggttaccgga
      601 tcgcggtgaa ctgcggcagg gacgggaccc agatcgtcat gcacctgcac ctccacgtcc
      661 tcggcggcag gctcttggac ggcaagctgg ggtgagcaag gcggggtgac gaagacccgg
      721 ggtgacgcct cgtcgtccat accgggaacg ggccccctcg cggcgccgcc catgcgatgg
      781 ccgagagggg cggccgctca gtcgaggtaa agcacgaacc cgccctggaa caatccttcc
      841 gggtgcgagg ggagccaggg gaaatctgcg ggcccggaca gggtctcgag aacgcgcacg
      901 tttcgagaca ggccagatgc gctcttgtgg acggccttga gcaggtccgg ccccgagaac
      961 tgcgcgttga aacccgcggc cacgaagagg atgcccggcg agcccaagcg cgccacggcg
     1021 cggtcgagga ccctcctgta ctcccggtac tccgacacgg acgcaggcgc gtccacaagc
     1081 acgaggtcgt atgaatccct gtccgcctcg aggaaactca cggcgtcgtc cctgtggtgt
     1141 ctccacacga aaggcctgat gcggttcagt tccatgttct tgcggagcag cgcctgggcg
     1201 ggggcagacg agtccacgga ggcgaccgag accgccccgg ccgcgagcgc gcacacggag
     1261 aaggaccccg agtacgagaa caggtcaagc accctgcggc ctttcgcgta cgcggcgatc
     1321 ctgctcctgg tgagcctgtg ctcgaggtag aacccggtcc tgtcgccggt gaggacgtcc
     1381 accgcatacg agaggccgtt ctcgctcacc accacatccc cgcccaagcc gccgcccttg
     1441 agcgggccga cctccgggcg gaggccgcgg cagccaaggc ccgccgcgac atcgccgaga
     1501 tgctggccga cctgcaggac cgcctcgcgg gcggcgcggc gctggagcag ctcgccgagg
     1561 agaccgcgat ggag
//
LOCUS       DAOSCR010000638         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53254_length_1570_cov_2.912211, whole genome shotgun sequence.
ACCESSION   DAOSCR010000638 DAOSCR010000000
VERSION     DAOSCR010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53254_length_1570_cov_2.912211"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..933
                     /locus_tag="PLT69_12310"
     CDS             76..933
                     /locus_tag="PLT69_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOJ15269.1"
                     /translation="MVDYINTSQPVMSDETFRLLRDFIYEHSGIFFPDNKKAQLENRL
                     ALRIRANGLEDFDKYYYLLRYDPDAARELRALFDSVTTNETSFFRSPPQIQAFQDKVL
                     PEIMAAKERRGEKTLRLWSAGCSTGEEPYTMAIVLSQVMNGRRDWDVKVVASDISEKA
                     LRSAKEAVYGEYSLRSVPPEIKRDFFVAAGSSYRIVDEVKDMVEFQFLNLSDTRRVQL
                     MRGFDVIFCRNVLIYFDDQVKKRFVSQLYDCLNHGGYLFIGHSESLHNISRAFKLVHF
                     PGALAYKKE"
     gene            945..1319
                     /locus_tag="PLT69_12315"
     CDS             945..1319
                     /locus_tag="PLT69_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15270.1"
                     /translation="MEKILIVDDSSTVRKFLATTLKTKNFRCVEASDGFDALEKLAQN
                     PDVRLIISDLNMPNMDGIEFISAVRKDPQFKDIPIIMLTTDASPENRKLAFETGANLY
                     LVKPSPAHIILFKVQSLLYADK"
BASE COUNT          344 a          508 c          454 g          264 t
ORIGIN      
        1 agatggagga catcggccag gaggtcaggg catacctgtc cgcgatatcg gacgaagaag
       61 acggggagcg tgccgatggt tgattacatc aacacctccc agcccgtgat gtcggacgag
      121 accttccggt tgctgcggga cttcatctac gagcactcgg gcatcttctt cccggacaac
      181 aagaaggccc agctcgaaaa ccggctggcc ctgcggatca gggccaacgg cctcgaggat
      241 ttcgacaagt actactacct gctcaggtac gatccggatg cggcccggga gctcagggcc
      301 ctgttcgact ccgtgaccac caacgagacg agcttcttca ggagccctcc ccagatacag
      361 gccttccagg acaaggtcct tcccgagatc atggcggcca aggagcgccg gggcgagaag
      421 acgttgaggc tctggtccgc cgggtgctcg acgggggagg agccgtatac catggccatc
      481 gttctgtcac aggtcatgaa cgggagacgc gactgggacg tgaaggtcgt cgccagcgac
      541 atcagcgaga aggcgctgcg ctcggcgaag gaggccgtct acggcgagta ttccctgcgc
      601 agcgtgccgc ccgagatcaa gagggacttc ttcgtggcgg caggttcctc gtacaggatc
      661 gtggacgagg tcaaggacat ggtcgagttc cagttcctga acctcagcga caccaggcgc
      721 gtccagctca tgcgggggtt cgacgtcatc ttctgcagga acgtgctcat ctacttcgac
      781 gaccaggtga agaagcggtt cgtctcgcag ctgtacgact gcctcaacca cggcgggtac
      841 ctcttcatcg gccactcgga gtcgcttcac aacatatcgc gcgcattcaa gctcgtgcat
      901 ttccccggcg ccctcgcgta caaaaaggaa tagcggagag tcccatggaa aagatcctca
      961 tcgtcgacga ttcgagcacg gtgcggaagt tcctcgccac gacgttgaag acgaagaact
     1021 tcaggtgcgt cgaggcgtcc gacgggttcg acgccctcga gaaactcgcc cagaatcctg
     1081 acgtgagact catcatctcg gatctgaaca tgccgaacat ggacggcatc gagttcatat
     1141 ccgccgtcag gaaggacccc cagttcaagg acatccccat catcatgctc accacggacg
     1201 cgtcgcccga gaaccgcaag ctcgccttcg agacgggggc gaacctctac ctcgtcaagc
     1261 cgtcgcctgc gcacatcatc ctgttcaagg tccagagcct cctctacgcc gacaagtgac
     1321 caccgctctc ccgggcgcat gacgcccgat cctcctgacc gtcacgccgg tcgacgagaa
     1381 cggttcacct tttctcaatc gatacccgaa gcggagaaga aagacccacc cgaaggcgtg
     1441 gcctcgcccg tacaagacat ccgcgaattc cctatccctg gcgctacgag aagaaagagc
     1501 cccccgaaga cgcggcctcg cccgagccgc cccccgccaa gacacgacct gccccctgga
     1561 cgacgaagag
//
LOCUS       DAOSCR010000639         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53299_length_1569_cov_3.467635, whole genome shotgun sequence.
ACCESSION   DAOSCR010000639 DAOSCR010000000
VERSION     DAOSCR010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53299_length_1569_cov_3.467635"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1569)
                     /locus_tag="PLT69_12320"
     CDS             complement(<1..>1569)
                     /locus_tag="PLT69_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ15271.1"
                     /translation="LMGSNMQRQAVPLLFPVSPLVGTGIEQLVARDSGVCVVAHRSGV
                     VIDVDSQRIVVKADTVGRKSDDTGIDIYRLTKFKRANQNTSFNQKPIVRKGQHVQKNQ
                     IIADGPATKDGELALGRNLTVAFMPWGGYNYEDAILISERVVKEDFYTSVHIEEFEVM
                     ARDVKLGSEEITRDIPNAGEEALKDLDESGIIRIGAEVKPGDILVGKVTPKGETQLSP
                     EEKLLRAIFGEKASEVKDTSLRVPPGIEGTVIDARVFNRRGQEKDERTLQIEQAEIEK
                     LRKDQDDEIDIITQGARARIEDLLVDKKLTAPLLSHLGDAVIGKKGTVITREMLSQLA
                     MDDFKDVAVENGEETAQEIEKIIASMERQKKLILDFYDAKIERLSAGDDLPPGVNKMV
                     KVYVAIKRKLSVGDKMAGRHGNKGVISRILPEEDMPFFADGRPVDIVLNPLGVPSRMN
                     VGQILEAHLGWAMHGIGEVINKMVEEHVPEEEIKGLLKKVYGSRRISRMLDKADREEL
                     LDLAAKIREEGFCV"
BASE COUNT          216 a          546 c          462 g          345 t
ORIGIN      
        1 ctcacgcaga aaccctcttc cctgatcttc gcagcgaggt cgaggagctc ctcgcggtcg
       61 gccttgtcga gcatcctgct tatcctgcgg gagccgtaca ccttcttcag caggcccttg
      121 atctcctcct cggggacatg ctcctccacc atcttgttga tcacctcccc gatgccgtgc
      181 atcgcccagc cgaggtgcgc ctcgagtatc tgcccgacgt tcatcctcga tggcacgccg
      241 agcgggttga gcacgatgtc caccggtcgg ccgtcggcga agaacggcat gtcctcctcg
      301 gggagtatcc tcgagatgac gcccttgttc ccgtggcggc ccgccatctt gtcgccgacg
      361 gagagcttgc gcttgatggc tacgtacacc ttcaccatct tgttcacgcc cgggggcagg
      421 tcgtcgccag ccgacagccg ctcgatcttc gcgtcgtaga aatccaagat gagcttcttc
      481 tgccgctcca tggaggcgat gatcttctcg atctcctggg ccgtctcctc gccgttctcc
      541 acggcgacgt ccttgaagtc gtccatggcc agctgcgaga gcatctcgcg ggtgatcacc
      601 gtgcccttct tgccgatcac cgcgtcgccc aggtgcgaca gcagcggcgc ggtcagcttc
      661 ttgtcgacga ggaggtcctc gatgcgggct ctcgcgccct gggtgatgat gtcgatctcg
      721 tcgtcctggt ccttgcggag cttctctatc tcggcctgct ctatctggag cgtgcgctcg
      781 tccttctcct gcccacgcct gttgaaaacc ctggcgtcga tgacggtccc ctcgatgccc
      841 ggcggcacgc gcagcgaggt gtccttgacc tcgctcgcct tctcgccgaa tatcgcgcgg
      901 agcagcttct cctcgggcga gagctgcgtc tcgcccttgg gcgtgacctt gcccacgagg
      961 atatcgcccg gcttgacctc ggcgccgatg cggatgatgc ccgactcgtc gaggtccttg
     1021 agggcctcct cacccgcgtt ggggatgtcg cgggtgatct cctcgctgcc gagcttcacg
     1081 tcgcgggcca tgacctcgaa ctcctctatg tgcaccgagg tgtagaagtc ttccttcacg
     1141 acccgctcgc ttatgaggat cgcgtcctcg tagttgtagc cgccccaggg catgaacgcc
     1201 acggtgaggt tccttcccag ggcgagctcg ccgtccttgg tcgccgggcc gtccgcgatg
     1261 atctggttct tctgcacgtg ctggcccttg cgcacgatgg gcttctggtt gaacgaggtg
     1321 ttctggttgg cgcgcttgaa cttcgtgagc cggtagatgt ctatgcccgt gtcgtccgac
     1381 ttcctgccca ccgtgtccgc cttcaccaca atcctctggc tgtccacgtc gatcacgacc
     1441 ccgctgcggt gcgccaccac gcagacgccc gagtccctcg ccacgagctg ctcgatgccc
     1501 gtgcccacga gaggcgagac ggggaagagc aggggcacgg cctgacgctg catgttcgac
     1561 cccatgagc
//
LOCUS       DAOSCR010000640         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53346_length_1568_cov_4.258427, whole genome shotgun sequence.
ACCESSION   DAOSCR010000640 DAOSCR010000000
VERSION     DAOSCR010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53346_length_1568_cov_4.258427"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLT69_12325"
     CDS             complement(<1..202)
                     /locus_tag="PLT69_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15272.1"
                     /translation="MDHGILVLDFITHPIYEFLHERFGLTDGNHFYQVTFTWLYMIFL
                     VLMGFLVSRGLKLVPGRGVCIMG"
     gene            complement(204..587)
                     /locus_tag="PLT69_12330"
     CDS             complement(204..587)
                     /locus_tag="PLT69_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOJ15273.1"
                     /translation="MRIAAKDPVQRRIEFIAYTLWALASVAGLSLSGADFALGVFLGG
                     LVCIANYHLLWRHARSSVTFPAKQGSGYMIRRYILRLALTGLILYVLIARLHIDVLGL
                     LLGLSVIMLGIMSYACYTYICAGGH"
     gene            complement(571..837)
                     /locus_tag="PLT69_12335"
     CDS             complement(571..837)
                     /locus_tag="PLT69_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ15274.1"
                     /translation="MGTISDKEKRDRKKLYLTVADLSTMGFAMVLSIVLGLVAGFFLD
                     SHLGTEPIFTFVFLFGGIMAGFRMMYKTYMRFFSEDKTDHEDRR"
     gene            1166..>1568
                     /locus_tag="PLT69_12340"
     CDS             1166..>1568
                     /locus_tag="PLT69_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOJ15275.1"
                     /translation="MNVPERYLAAYPVIGYTSSESLPERILRAGEGEDRKRILVEVLL
                     PMVLKANERVFAQRMVVERIARSLPSLDRHEEETLWALARIYRVEATTREEAVRELLM
                     RVDVLPPSLVLAQAAIESGWGTSRFSHDGNNV"
BASE COUNT          344 a          432 c          494 g          298 t
ORIGIN      
        1 cgcccatgat gcacacgccc cggccgggca cgagcttcag gcccctggag accaggaagc
       61 ccatcagcac gaggaagatc atgtagagcc aggtgaaggt gacctggtag aagtggttcc
      121 cgtctgtcag cccgaagcgc tcgtgcagga actcgtagat ggggtgcgtg atgaagtcaa
      181 ggacgagaat gccgtgatcc atactaatgt cctcctgcgc agatgtaggt gtagcacgcg
      241 tagctcatga tcccgagcat gatcacggac agcccgagca agaggcccag gacgtctatg
      301 tggagccggg cgatgagcac gtagagaatc aggccggtca gcgccagcct gaggatgtat
      361 cgcctgatca tgtagccgga accttgcttt gccgggaagg tcaccgagga tctcgcgtgt
      421 ctccagagga gatggtagtt ggcgatgcac accaagcccc cgagaaaaac gccaagggcg
      481 aagtccgctc ccgacagcga caggcccgcg acggacgcca aggcccagag ggtgtaggcg
      541 atgaactcga tacgcctctg cacggggtct ttagcggcga tcctcatggt cggtcttgtc
      601 ctcgctgaag aacctcatgt aggtcttgta catcatccgg aagcctgcca tgatgccgcc
      661 gaagaggaac acgaaggtga agatgggctc ggtgccgagg tgggagtcga ggaagaaccc
      721 ggccacgagc ccgagcacga tcgaaagcac catggcgaaa cccatcgtgc tcaggtccgc
      781 aacggtcagg tagagcttct tgcggtccct cttctccttg tcgctgatag tacccatggg
      841 tagagatatc ctctcgacga agtgaacacc atactactga atcgtcattc ataatgcaac
      901 agagattttc tcggcgccgt accggctata acctaaaggt atcattggtt aaaatagcat
      961 gccgccggcg aggtatattt ttcttgccat gtgaaagatt ttgagataaa caattgaagt
     1021 ttgaggatat gaaagcgtta acgtgtgaca tatggacggt tcacggactg acaaggctca
     1081 ggtgcgcacc cgggctctca gcttgacgtg ttttctggcg tcattcgtcg tcgcatcgtt
     1141 gatacccgcc gagatcagca cgggtatgaa cgtgcccgag aggtatctgg cggcataccc
     1201 cgtcatcggg tacacctcct cggagagcct gcctgagcgg attctccggg cgggagaggg
     1261 cgaggacagg aaacgcatcc tcgtcgaggt gctcctgccc atggtgctca aggcgaacga
     1321 gcgggtgttc gcgcagcgca tggtggtcga gcgcatcgcc aggtctctcc cctccctcga
     1381 caggcacgag gaggagacct tgtgggcctt ggcgcgcatc tacagggtgg aggcgacgac
     1441 cagggaggag gccgtgcgag agcttctcat gcgggtggac gtcctccccc cttcgctcgt
     1501 cctggcgcag gccgccatcg agtcggggtg ggggacctcg aggttcagcc acgatgggaa
     1561 caacgtct
//
LOCUS       DAOSCR010000641         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53407_length_1567_cov_3.005291, whole genome shotgun sequence.
ACCESSION   DAOSCR010000641 DAOSCR010000000
VERSION     DAOSCR010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53407_length_1567_cov_3.005291"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /gene="lptA"
                     /locus_tag="PLT69_12345"
     CDS             complement(<1..468)
                     /gene="lptA"
                     /locus_tag="PLT69_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOJ15276.1"
                     /translation="MICSLFAATAVSGADLKAHERGTDTVIIEADRLDVNATQSTAVF
                     KGNVRAARGTMSLRADTVTLVYDKQTRKVDTLSASGKVSITWDDRRAECLRLVYHVQD
                     QLMELIGDVTITKGRESLSGQRVTVDLKRDTQTAEGGDERVRERVEDQAVSRIL"
     gene            complement(458..985)
                     /locus_tag="PLT69_12350"
     CDS             complement(458..985)
                     /locus_tag="PLT69_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15277.1"
                     /translation="MSRYAALAAAVLLCALILVLQSPEGDKTEGGNSPRVRRLLNVTM
                     VERSHTTGRTVRITASEVIEDHLGTAHLVGLKAEDAGGSFLEAPRAVYDMKTSVLKVE
                     GPTSITAGGWLTMKLNGISWDLENDSGGTAEAVTIEGRGSVITAPRAAFSSRFSTITL
                     SGGVHAEISRDVLDL"
     gene            complement(978..1481)
                     /locus_tag="PLT69_12355"
     CDS             complement(978..1481)
                     /locus_tag="PLT69_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOJ15278.1"
                     /translation="MNRISCMVFDVDGVLTDGGITITDAGDEIKTFNSRDGHGIKLLL
                     RAGIQVAIITGRSSRVVEHRARELGIQYVIQGSKDKKEALLDLAERLGIEPSNMAYMG
                     DDVVDLPAMALCGLSFAPADALEVVKERSDVVTAREGGKGAAREAAEILLKRMGLYEQ
                     VMERYGV"
BASE COUNT          237 a          540 c          464 g          326 t
ORIGIN      
        1 caggatccgc gagaccgcct ggtcctccac ccgttccctc accctctcgt ctccaccctc
       61 tgcagtctgc gtgtccctct tgagatccac cgtcacccgc tggcccgaga gactctctcg
      121 gcccttggtt atggtcacgt cgcctatgag ttccatgagt tggtcctgga cgtggtacac
      181 gagccgcaag cattcggcac gcctgtcgtc ccaggtgatg gacaccttcc ccgatgcgct
      241 gagggtgtcg accttccggg tctgcttgtc gtacacgagc gtcacggtat cggccctgag
      301 gctcatggta ccccttgcgg ccctgacgtt gcccttgaac accgccgtgg attgcgtcgc
      361 gttcacgtcc aacctgtccg cttctatgat caccgtatcg gtaccccttt cgtgtgcctt
      421 gaggtccgca ccggagacgg ccgttgccgc gaagaggcta cagatcaaga acgtcgcgag
      481 atatctctgc atggactccg ccggaaaggg ttatggttga gaacctcgat gagaaggccg
      541 cccgtggggc cgtgatcacg ctccccctgc cctcgatggt gaccgcctcc gcagtgccgc
      601 cggagtcgtt ctccaggtcc cacgagatgc cattcagttt catggtcagc cagccgccgg
      661 ccgttatgga cgtcgggcct tccaccttga gaacagaggt tttcatgtcg tagacggcac
      721 gaggcgcttc caggaaggat cccccggcat cctcggcctt cagcccgacg aggtgcgccg
      781 tcccgaggtg gtcctcgatc acctcgctcg ccgttatcct gaccgttctg ccggttgtgt
      841 gcgacctctc caccatggtc acgttcagga gcctgcgtac ccgtggggag ttgcccccct
      901 cggtcttgtc cccctccggt gactggagga cgaggataag ggcgcagagc agcacggccg
      961 cggcgagggc ggcataccta gacaccgtag cgctccatga cctgttcgta gaggcccatc
     1021 cgtttcaaca ggatttccgc ggcctcccgg gccgcccctt tcccgccctc ccgggcggtc
     1081 accacgtcgg aacgctcttt caccacctcg agggcgtccg ccggtgcgaa gctcaggccg
     1141 cacagggcca tggcggggag gtccaccaca tcgtccccca tgtaggccat gttcgagggt
     1201 tctatgccga ggcgctcggc cagatccaaa agcgcctcct tcttgtcctt ggagccctgg
     1261 atcacgtact ggatgccgag ctctcgagcc cggtgctcca ccacgcggga cgacctcccg
     1321 gtgatgatgg ccacctggat gcctgcgcgc agcagcagct tgattccgtg gccgtccctg
     1381 ctgttgaacg tcttgatctc gtcccccgcg tcggtgatgg tgatgccccc gtcggtgagc
     1441 accccgtcca cgtcgaagac catgcaggag atcctgttca tccggaggcc tctcgtatcc
     1501 tgagcaggct ctcgatgagg ggttcgagac ggtcgagcct gaggctgttg gggccgtcgc
     1561 tcttggc
//
LOCUS       DAOSCR010000642         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53515_length_1565_cov_3.334437, whole genome shotgun sequence.
ACCESSION   DAOSCR010000642 DAOSCR010000000
VERSION     DAOSCR010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53515_length_1565_cov_3.334437"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..302
                     /locus_tag="PLT69_12360"
     CDS             102..302
                     /locus_tag="PLT69_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15279.1"
                     /translation="MSDRSLCPICAWRGDCNKKFKAGAGVHCPDFSRDLTIKNPELEA
                     EADRQADQKSGAKKPKKGLWPI"
     gene            complement(310..>1565)
                     /locus_tag="PLT69_12365"
     CDS             complement(310..>1565)
                     /locus_tag="PLT69_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15280.1"
                     /translation="VDPQGQAGGGVSEEWLSTTIEARLIARGVPKEVYETLSVRVTTL
                     GKALSLDAVLRTAPPRAFHKDIPGSDAVSGTLDEMIDAIFPKGPGETQPETTKSPEGP
                     KAPATGEAPPQAAPSQPHPKTQEGLASVSLPFMPTSIASSGNDLFSSDRKTVYRIEGG
                     ETRQVWKAPGAWEILRLYPYEGSLLVLAASNGVLRTFSVRGDKTEKTWNKAVLPVGDG
                     LVSTYLRFDDETADPVFPFSPASREAGSPVLPPGKVDPLSAAALSPGRGSMSHRIVSF
                     DRDDRLTVLEGKDVLWVSGKAAGATPLFVNAPTKRERRPGSDSSPSASTYYVLRPRIV
                     VAGSRVLTFRNEQGLGSMLPRLGLFTSSEVLAYEPTGDGFQPRVLARFPDGHCVDVAV
                     AGSKAAVLVVEGKQATVRFLDLSGY"
BASE COUNT          277 a          516 c          497 g          275 t
ORIGIN      
        1 tgtccataag ccaacttata gcacttttca cgtgccaaag ttgtcatgaa ccttttcttt
       61 tcccgttgac aaggcggctt tcgctcccct accttgtagc catgtccgac agatcactgt
      121 gccccatatg cgcgtggaga ggcgactgca acaagaagtt caaggcaggt gcaggagttc
      181 actgccccga cttcagccgg gaccttacga tcaagaaccc cgagctcgag gccgaggctg
      241 accggcaggc agaccagaag agcggcgcca agaagcccaa gaagggcctc tggcccatct
      301 gagcgcctgt caatagccgg acaggtcgag gaacctgacg gtcgcctgct tgccctcgac
      361 gacgaggaca gcggccttgc tcccggccac ggccacatcc acgcagtgtc catccggaaa
      421 acgcgcaagg accctgggtt ggaagccgtc gcccgtcggc tcgtaggcga gcacctctga
      481 cgaggtgaac aggccgagcc tcggcagcat ggagccgaga ccctgttcgt tcctgaacgt
      541 cagcaccctc gaccccgcga ccacgatcct cggtctcagg acgtagtacg tcgacgcgga
      601 agggctcgag tctgacccgg gcctcctctc ccgcttggtc ggtgcgttta cgaagagagg
      661 cgtggcgcct gcggccttgc cggataccca caggacatcc tttccttcga ggaccgtgag
      721 cctgtcgtcc ctatcgaagg agacgatgcg gtgcgacatc gatcctcggc cggggctcaa
      781 cgcagccgcg ctcaaggggt ccaccttccc gggcggcaac accggtgaac ccgcctcccg
      841 ggaggccggg gagaacggga acacggggtc ggcggtctcg tcatcgaacc tcaggtaggt
      901 ggagaccagg ccgtcaccga cgggcagcac ggccttgttc caggtcttct cggtcttgtc
      961 tccgcggacc gagaaggtgc gcagcacccc gttcgatgcg gccagcacga gcagcgagcc
     1021 ctcgtagggg tacagtctca ggatctccca tgcgccgggc gccttccaga cctgccgggt
     1081 ttcgccgccc tcgatcctgt aaaccgtctt tctgtcgctc gagaagagat cgttccccga
     1141 agaggcgatc gaggtcggca tgaaggggag gctcaccgat gcgaggcctt cctgggtctt
     1201 tggatgcggc tgggaggggg cggcctgtgg gggtgcctca cccgtggccg gggccttggg
     1261 gccctcaggc gacttcgtcg tctcgggctg cgtctccccc ggtcccttcg ggaagatggc
     1321 atcgatcatc tcgtcgaggg ttccggaaac ggcgtccgag ccaggtatgt ccttgtgaaa
     1381 tgcgcgcggc ggagcggtac ggagcacggc gtccaaggac agggccttcc cgagcgtggt
     1441 cacgcgcaca gagagggttt cgtagacctc cttcggcacc ccccgggcta tcaagcgggc
     1501 ctcgatcgtc gtcgacagcc attcctcgga gacaccccct ccagcctgcc cctgcgggtc
     1561 gacct
//
LOCUS       DAOSCR010000643         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53770_length_1560_cov_3.946844, whole genome shotgun sequence.
ACCESSION   DAOSCR010000643 DAOSCR010000000
VERSION     DAOSCR010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53770_length_1560_cov_3.946844"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1560
                     /locus_tag="PLT69_12370"
     CDS             <1..>1560
                     /locus_tag="PLT69_12370"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOJ15281.1"
                     /translation="SPGPCLAYMLDVEADSIKRDRERIEAWGNVIVKGRDVTLTADRV
                     TYDTKTEEILATGSCRLEDSRVVVRAETISYNAQTGDLVLGRGSLSSVEGSMRITGES
                     IERFESDHIRAQNVEFTSCLGEPPDWSLFVGDLDIPLGGYGQGRDVRFRVRDHTLVYF
                     PWLFFPAKFYRHSGVLFPEMGHGSDYGYRLGLPLYLVLSRSADMTVTPTYLSQRGLLV
                     KAEARYCVDAAQSGFLYLEGLKDREGGQEKAGTIEQAPPDERWFLKARHDGTPLRWDV
                     NLASTADYFRDIGAFMNDNPLQERDASPELNDLGDSRMEALVSRAQWAAGAGPLSWSV
                     SGIYTQDLTVKDNGLTVQQLPRTTLRLSQWRVPTTPLMTSAGIGAVRAVTQDASDAVE
                     NDAWAELAMPVGVQPYFTITPSVREYYRRTRTTSAGASLHEEDTGERWQERKVTLSTA
                     LYGQKMGSGLRHQVVPGVTWLYRSERGDARERDADPGGCPWDIDLGRIDESNDVTVSL
                     ANYLRDASGGSL"
BASE COUNT          294 a          490 c          534 g          242 t
ORIGIN      
        1 tcgcccgggc cttgcctcgc ctacatgctc gacgtggagg cggactcgat caagagggac
       61 agggaaagga tcgaggcctg gggcaacgtg atcgtgaagg gcagggacgt cacgctcacg
      121 gccgaccgtg taacctacga cacgaagacc gaggagatcc tcgcgacagg ctcgtgcagg
      181 ctcgaagaca gcagggtggt agtcagggcc gagaccatca gttacaacgc gcagacgggc
      241 gacctggtcc tgggcagggg gtcgctctcg tcggtcgagg ggagcatgag gataacgggc
      301 gaatccatcg agcggttcga gagcgatcac atccgggccc agaacgtcga gttcacctcg
      361 tgcctgggag agcctcccga ctggtctctc ttcgtgggcg atctcgacat cccgctcggc
      421 ggctacggcc agggaaggga cgtcaggttc agggtgaggg accataccct tgtctatttc
      481 ccctggctgt tcttcccagc gaagttctac aggcactcgg gcgtgctctt ccccgagatg
      541 gggcacgggt cggattacgg gtaccgcctc ggcctcccct tgtacctggt cctctcgagg
      601 tccgcggaca tgacggtcac gcccacgtac ctctcccaga gggggctcct ggtcaaggcc
      661 gaggcgcgct actgcgtgga cgccgcacaa tctgggttcc tctacctcga gggcctcaag
      721 gacagggagg gtgggcaaga aaaggctggc acgatcgagc aggcgccgcc cgatgaacgc
      781 tggttcctga aggcccggca cgacgggacg ccgctcaggt gggacgtgaa cctggccagc
      841 acggccgact atttcaggga cataggcgct ttcatgaacg acaaccccct ccaggagaga
      901 gatgcctcgc ctgaattgaa cgatctcggc gacagcagga tggaggccct cgtgtcccgt
      961 gcccagtggg ccgcaggcgc ggggccgctg agctggtccg tctccgggat atacacccag
     1021 gacctcacgg tgaaagacaa cggcctcacg gtgcagcagc tgccgaggac cacgctcagg
     1081 ctctcgcagt ggagggtccc caccaccccg ctcatgacct cggccgggat cggcgccgtg
     1141 cgcgccgtga cccaggatgc ctccgacgcg gtggagaacg acgcatgggc cgagctcgcc
     1201 atgccggtgg gagttcagcc atacttcacg atcacgccgt ctgtgcggga atactaccgg
     1261 agaacccgga ccacctccgc gggggcctcc ctgcacgagg aagacaccgg cgagaggtgg
     1321 caggaaagga aggtgaccct ctccaccgcc ctgtacgggc aaaagatggg gtcgggcctc
     1381 aggcaccaag tcgtgccggg cgtcacgtgg ctgtaccgct cggagcgcgg ggatgcccgg
     1441 gagagggatg ccgaccccgg gggctgcccc tgggatatcg acctggggag gatcgatgag
     1501 tcgaacgatg ttaccgtaag cctcgcgaac tacctcaggg atgcctcggg cggctccctg
//
LOCUS       DAOSCR010000644         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_53839_length_1559_cov_3.199468, whole genome shotgun sequence.
ACCESSION   DAOSCR010000644 DAOSCR010000000
VERSION     DAOSCR010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53839_length_1559_cov_3.199468"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLT69_12375"
     CDS             complement(<1..302)
                     /locus_tag="PLT69_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOJ15282.1"
                     /translation="MALLGTLKGFGVTEIFQLISQQMKTGSLVLTSTKATVTINFENG
                     VIVGLSTNTWERDPMADVLMKAGLLQEKDFKAALESAKKNSQPWYEILVSKGQLKRV"
     gene            complement(304..>1559)
                     /locus_tag="PLT69_12380"
     CDS             complement(304..>1559)
                     /locus_tag="PLT69_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15283.1"
                     /translation="CLVFALPLFFDVGAGILVAWWFLVLWNYLLASEKRIVYAFIFVV
                     LISGWLTHVAAGFITYPETQLSREMFSVQNRSATQADLDSLASWSRQHPDDAEPLNAL
                     ALHAIARSEWQEGIRLLEACIDLEPANPRYYNHLAVALMGTGRTKEALKALDYAAKLK
                     PDSVIYYYNMSRIYQSTYNFYESESAIAQASGIDSRLVRQLLDTENKVGRTRLILEKT
                     PVSRYLARQMRPSPVLGEAADALWHWVFGLIPRRASAFLGAGFFVTLLLMSFIPRDKF
                     SKQCARCGKVYYSGTFTKSGSPMCLQCSWLDSKAKKQEVTIVQHKSEDIRSFKAQSFR
                     KLLRLDMVLPGLGCFMAHRTGTALIRLVVLCACLAALAMGSGVIVSFVPVHVNAGLAI
                     RTAAVVVWASLLLRVYRRPPLRFGG"
BASE COUNT          288 a          463 c          509 g          299 t
ORIGIN      
        1 acccgtttga gctggccctt ggatacgagg atctcgtacc agggctggga gttcttcttg
       61 gcgctctcca gggcggcctt gaagtccttc tcctggagga gccccgcctt catgagcacg
      121 tcggccatgg ggtctcgttc ccatgtgttc gtactcagac cgacgatgac cccgttctcg
      181 aagttgatgg tgaccgtggc cttcgtcgag gtgagcacga gagaccccgt cttcatctgc
      241 tgggagatca gctggaagat ctccgtgaca ccgaaaccct tgagtgtgcc cagaagcgcc
      301 atgtcagcct ccgaacctga ggggagggcg cctgtacacc ctcagcagga gtgacgccca
      361 gacgactacg gcagccgttc gaatcgcaag cccggcgttt acgtggaccg gcacgaagga
      421 tacgatgacg ccgctgccca tggcgagcgc cgcgaggcat gcgcacagga cgacgagcct
      481 gatgagggcc gtgcccgtgc ggtgggccat gaagcagccc agccccggca ggaccatgtc
      541 gagcctgagg agtttccgga agctctgggc cttgaaactc ctgatgtcct ccgacttgtg
      601 ctggacgatc gtcacttcct gcttcttggc cttcgagtcg agccacgagc attgcaggca
      661 catcgggctg ccggatttcg tgaacgtgcc ggagtagtac accttcccgc accgggcgca
      721 ttgcttcgag aacttgtccc tgggaatgaa gctcatgagg aggagggtga cgaagaaccc
      781 cgctcccaag aaggccgagg cgcgtctcgg tatcagcccg aagacccagt gccacaaggc
      841 gtccgcggcc tccccgagga caggggaagg cctcatctgc cgggcgaggt acctcgagac
      901 cggggtcttc tcgaggatga gacgggtcct gcctaccttg ttctctgtgt caaggagctg
      961 cctgacgagc ctggagtcta tcccgctcgc ctgtgcgatc gcgctttcgc tctcgtagaa
     1021 gttgtaggtg gactggtaga tcctgctcat gttgtagtaa tagatcacgg agtccggctt
     1081 cagtttggcg gcgtagtcga gcgccttgag ggcttccttg gtcctgccgg ttcccatgag
     1141 ggcgacggcg agatggttgt aatagcgggg gttcgcgggc tccaggtcta tgcaggcctc
     1201 aaggagcctg atcccctcct gccattcgga cctggcgatg gcatgcagcg cgagcgcgtt
     1261 caagggctcg gcgtcgtccg ggtgctggcg cgaccacgag gcgagggagt cgaggtcggc
     1321 ctgggtcgcg ctccggttct ggaccgagaa catctccctg ctgagctgtg tctcggggta
     1381 cgttatgaag cccgccgcca cgtgggtgag ccaccccgat atgagcacga cgaagatgaa
     1441 tgcatacacg atgcgcttct cggatgccaa caggtagttc cacagaacga ggaaccacca
     1501 ggccacgagt atgcctgcgc ccacgtcgaa gaacaggggg agggcgaaga cgaggcaca
//
LOCUS       DAOSCR010000645         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54025_length_1555_cov_3.470667, whole genome shotgun sequence.
ACCESSION   DAOSCR010000645 DAOSCR010000000
VERSION     DAOSCR010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54025_length_1555_cov_3.470667"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PLT69_12385"
     CDS             <1..891
                     /locus_tag="PLT69_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ15284.1"
                     /translation="ERLAAETCVESITGVDIRRPGFSSPRLEFLARDVRDPLDPVFEG
                     RNIDTVVHAAYILPPIHDTALMEDINVNGTKNVFQTAVRHNVEQVVECSSATAYGFHP
                     DNPPVLTEESPLKGNEDFTYAKNKREIEEWLEGFSAEHPETLFTVIRPCFVVGPGFAN
                     PLARHLCRKICMLPDKTAPFQFIHEDDLVEIIFLLAKNRKGGVYNLAADGTMTFDEML
                     AMLGSIPLRLPLWLLGPLNEIAWKLRMRFLTEFPSSSLKLTIYPWIVSNEKVKRELGY
                     AFRYTTREAFEDFVRSVKGR"
     gene            908..>1555
                     /locus_tag="PLT69_12390"
     CDS             908..>1555
                     /locus_tag="PLT69_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ15285.1"
                     /translation="MDPKGRHVLVTGAAMGIGRALASRFAERGANLVLVDLSAQEAAL
                     SEFAARLAARHGTRTLKCLCDLTEDDGPERVYQSVAAAGIEVSILVNNAGVCWYGRFG
                     DMPPERLESVILLNCMAYAKMCRLFLPPMIARDEGGVLNVSSVSAFQPVPMLALYAAT
                     KAFTQSLTEAVRLELPPRSKVVVATLNPPFTRTHLIEDAGVPSDYVPVKMSLMDAD"
BASE COUNT          298 a          491 c          501 g          265 t
ORIGIN      
        1 gaacgccttg ctgccgagac ctgcgtcgag agcatcaccg gcgtcgacat ccggcggccc
       61 gggttctctt cgcccaggct cgagttcctg gccagggacg tgagggaccc gctggaccct
      121 gtcttcgagg ggaggaacat agacaccgtt gtacacgccg cctacatcct gccacccatc
      181 cacgacacgg ccctcatgga agacatcaac gtgaacggca cgaagaacgt ctttcagacc
      241 gccgtgcgcc ataacgtgga gcaggtcgtc gagtgctcgt cggccaccgc ctacgggttt
      301 caccccgaca acccgccggt cctgaccgag gaaagcccgc tcaagggaaa cgaggacttc
      361 acctacgcga agaacaagcg cgagatcgag gagtggcttg aggggttctc agccgagcac
      421 ccggaaacgc tcttcacggt cataaggccc tgtttcgtgg tgggcccggg gtttgcgaac
      481 ccgctcgcca ggcacctctg caggaagatc tgcatgctgc ccgacaagac ggcccccttc
      541 cagttcatcc acgaggacga tctcgtggag atcatcttcc tgctcgcgaa aaacaggaag
      601 gggggggtct acaacctcgc cgcagacggg accatgacgt tcgacgagat gctcgcgatg
      661 ctcggctcca tccccctgag gctccctcta tggctcctgg ggccgctcaa cgagatcgcc
      721 tggaagctca ggatgaggtt cctcacggag ttccccagct ccagcctgaa gctcaccata
      781 tacccgtgga tcgtgagcaa cgaaaaggtc aagcgggagc tgggatacgc gttcaggtac
      841 accacgaggg aggcgttcga ggatttcgtg cgctccgtga aggggcgctg acatgcgagg
      901 aggtgacatg gatcccaagg gcaggcatgt gctggtgacg ggcgcggcca tgggtatcgg
      961 ccgcgcgctg gcttcccggt tcgcggagag gggggcgaac ctggttttgg tggatctctc
     1021 cgcccaggag gcggcccttt cggagttcgc agcccggctt gcggccaggc acgggacgag
     1081 gaccctgaag tgcctgtgcg acctcacgga ggacgacggg cctgaacgtg tgtatcaaag
     1141 cgtggctgcg gcagggatcg aggtgagcat cctggtgaac aacgcaggcg tgtgctggta
     1201 cggaaggttc ggggacatgc cgccggagcg cctggagtcg gtaatcctgc tcaactgcat
     1261 ggcgtacgcg aagatgtgca ggctctttct cccccccatg atcgcgaggg acgagggggg
     1321 tgtcctcaac gtatcctcgg tctccgcctt ccagcccgtg ccgatgctcg ccctctacgc
     1381 ggcgacgaag gccttcaccc agagcctcac cgaggccgta cgcctggagc tcccaccgag
     1441 gagcaaggtg gtcgtcgcga cgctcaaccc gcccttcacc aggacgcatc tcatcgagga
     1501 tgcaggcgtg ccgtcggact acgtccccgt gaagatgtcc ctcatggacg cagac
//
LOCUS       DAOSCR010000646         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54234_length_1551_cov_4.252005, whole genome shotgun sequence.
ACCESSION   DAOSCR010000646 DAOSCR010000000
VERSION     DAOSCR010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54234_length_1551_cov_4.252005"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLT69_12395"
     CDS             complement(<1..827)
                     /locus_tag="PLT69_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15286.1"
                     /translation="MPSPDIPTRYPKEIVDLARVFSSPMLLGPPMGEPLLALVNHLFS
                     PEEACVALHIPRYIPTPLEKIAKKAARDPGEIKVLLEAMAERRVIWSSERGYCLLPLI
                     PGMFEYLLMDGRDSPWHREYARLINALCASGYQREYYRNKSPLIRNIPVDEQVAVHGK
                     VMDEVTMSRMIEDYDRMAVLNVCQCRQSLAFAGEKCLRSSPLDGCLVFGSFADTVVER
                     KSGRYVSKEEMRSIVRDRWEKNLVFMTANVSHAGPNAICTCCSCCCHFLESVNRFGGT
                     "
BASE COUNT          285 a          480 c          503 g          283 t
ORIGIN      
        1 gtccccccga accggttgac ggactcgagg aagtggcaac agcacgagca gcacgtgcag
       61 atggcgttcg ggcccgcatg cgagacgttc gccgtcatga acacgaggtt cttttcccag
      121 cgatcccgca cgatggagcg catctcctcc ttgctcacgt agcggccgct cttgcgttcg
      181 accaccgtgt ccgcgaagct gccgaagacc aggcagccgt ccagggggct cgagcggagg
      241 catttctcgc ccgcgaacgc aagggactgg cggcattggc acacgttgag gaccgccatg
      301 cggtcgtagt cttcgatcat cctcgacatg gtgacctcgt ccatgacctt gccgtgcacg
      361 gcaacctgct cgtccacggg gatgttcctg atcagggggc tcttgttcct gtagtactcc
      421 ctctggtagc cgctcgcgca gagggcgttg atcagcctcg catactccct gtgccacggc
      481 gaatccctgc cgtccatgag caggtactcg aacatgccgg ggatgagagg cagcaggcag
      541 taccccctct cggaagacca gatgacccgc cgctctgcca tggcctcgag gagcaccttg
      601 atctcccccg gatctcttgc cgccttcttg gctatcttct cgaggggcgt cgggatgtac
      661 ctgggtatgt gcagggcgac acaggcctcc tccggggaga acaggtggtt gacgagggcc
      721 aagagcggtt cccccatggg cgggcccaac agcatgggcg agctgaagac cctggccaga
      781 tcgacgatct ctttcgggta ccgagtcggt atgtcgggcg aaggcatgga ggccaccttt
      841 ctgaaccata tccgtgtcaa cggctgagaa ttcttgtagc acaaaacgtg tcgcaaagaa
      901 cccctgtcaa aacactccat ggacgcatgc acggttttct ccagagagcg gcctcccgga
      961 ttcgttggcg tgcgctgcag cgcttcacgg gcggaaaggc cgtgaggtcg gccgggcgtt
     1021 ccttgagggt tcgcggctcg agaggctcac gccggaagaa ggaaacgcga tctgtcaagg
     1081 gcctccccca tgcgttccgc agcctcgatg cagatgatgc agatccggac gcgcagtgcg
     1141 tgcagggcgc gtacagcccg caggacgccc tgtcgagtga ggtcccttga gaagaggtcg
     1201 gagagtgcct gtgcggaagc gacaggagtt ttccttgagg gagagggggg tctcccggct
     1261 gaaggctgcc gctctggtgc atgtgcacgg tctggtccgg ccacggcgag ggttggacag
     1321 gcgaagacag ccaagctgta ggatgggttg tgtacccgaa cgtgtcgctc gagcgtcttc
     1381 ctacatccag ccgatccttc gcatcgcgct caccaccaga gagacgccca ccagcgtgac
     1441 gaacgccccg aaggcgagtc tcagcgttgg gcccgagagc ctgttggcga gacgggcgcc
     1501 tgcccatgcg ccgactatga gcgcgagggc gatgatgacg cccgccttca c
//
LOCUS       DAOSCR010000647         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54392_length_1548_cov_3.907569, whole genome shotgun sequence.
ACCESSION   DAOSCR010000647 DAOSCR010000000
VERSION     DAOSCR010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54392_length_1548_cov_3.907569"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLT69_12400"
     CDS             complement(<1..345)
                     /locus_tag="PLT69_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein"
                     /protein_id="HOJ15287.1"
                     /translation="METASVKRCIALYSGGLDSILAIRLLERQSFEVAALYFCTPYFG
                     LDALLDPEAFEEKAFKRYGVRVKVVDFTEDMAAIIRAPRHGYGKHLNPCIDCKIGMLR
                     RARGLLETENASF"
     gene            complement(520..597)
                     /locus_tag="PLT69_12405"
     tRNA            complement(520..597)
                     /locus_tag="PLT69_12405"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(560..562),aa:Asp,seq:gtc)
     gene            complement(615..690)
                     /locus_tag="PLT69_12410"
     tRNA            complement(615..690)
                     /locus_tag="PLT69_12410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Val,seq:tac)
     gene            complement(709..984)
                     /locus_tag="PLT69_12415"
     CDS             complement(709..984)
                     /locus_tag="PLT69_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ15288.1"
                     /translation="MTKTELISVVANKAGIPKAAAEKALNATLDAIQDALKKGDKVTL
                     VGFGTFDVAKRAARQGVNPRTREAIKIKASKSPRFKAGKAFKEAVNK"
     gene            complement(1068..>1548)
                     /locus_tag="PLT69_12420"
     CDS             complement(1068..>1548)
                     /locus_tag="PLT69_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOJ15289.1"
                     /translation="ETTVVPGKGKLLLTGKMGEVMQESAQASMSYVRSRAQSLGLDAN
                     FYDKLDIHIHIPEGAVPKDGPSAGVTMATAVVSALTSRSVKADLAMTGELTLGGRVLP
                     VGGLKEKLLAAKEAGVTTVLIPAENEKDLSDTPREILKGIKVHLVEHMDEIIALALN"
BASE COUNT          265 a          502 c          448 g          333 t
ORIGIN      
        1 gaacgatgcg ttctcggtct cgaggagccc tctggcccgc ctcagcatcc cgatcttgca
       61 gtctatgcag gggttcaggt gctttccgta cccgtgcctc ggcgcgcgga tgatcgcggc
      121 catgtcttcc gtgaagtcga cgaccttcac cctcaccccg tacctcttga aggccttctc
      181 ctcgaaggcc tcggggtcga gcagcgcgtc gaggccgaag tagggcgtgc agaagtagag
      241 cgccgccacc tcgaacgact ggcgctccag gagcctgatg gccaggatgc tgtcgagacc
      301 ccctgagtag agcgctatgc acctcttgac ggacgccgtc tccatgaccc accacgtcta
      361 ccccaaacag gcctgcatga acagagtcct tttgcccgga accgacgctc gtggacgtgt
      421 cttctctctt ctatcgggac ccatgcacgg gggcgggggt gtgctttcga gaaacgaaag
      481 tctatacccc acgaagaagc atggggtata gaccgtgcat ggcgggagcg acgagacttg
      541 aactcgcgac ctccggcgtg acaggccggc gttataaccg atcttaacta cgcccccgta
      601 ctctctcgtc ttcttggtag gcggaacagg gtttgaacct gtgaccttcg gcttgtaagg
      661 ccgacgctct cccggctgag ctacccgccc tgaacgtagt cttggccgct acttgttgac
      721 ggcttccttg aaagcctttc ccgccttgaa gcgcggcgac ttggaggcct tgatcttgat
      781 cgcctccctg gtcctcgggt tgacgccctg acgcgcggcc ctcttcgcca cgtcaaaggt
      841 gccgaagccc accagggtca ccttgtcacc cttcttgagg gcgtcctgaa tagcgtccag
      901 tgtagcattg agcgcctttt cggccgccgc cttggggatg cccgccttgt tcgccacaac
      961 ggatatcaat tctgtctttg tcaaagcata cctcctagaa agttctttat tttcccatga
     1021 ccgcgcgtcc tggaacggta acaatatttg aaaatcctgt caatagctca attgagggcg
     1081 agcgcgatga tctcgtccat atgttcaacg agatgcacct tgatcccctt gaggatctcc
     1141 ctcggggtgt ccgagaggtc cttctcgttc tcggcgggga tgagcaccgt cgtgaccccg
     1201 gcctccttgg ccgcaaggag cttctccttg agcccgccga cgggcaggac ccggccgccg
     1261 agcgtcagct cgccggtcat ggcgaggtcg gccttcacag aacgcgaggt gagggcggaa
     1321 accacggccg tggccatggt gacgcccgcg gacggcccgt ccttcggcac cgccccctcg
     1381 ggaatgtgga tgtggatatc cagcttgtcg tagaagttcg cgtccaggcc caggctctgt
     1441 gcgcgcgatc tcacgtagct catgctcgcc tgggcggact cctgcatgac ctctcccatc
     1501 ttgcccgtga gcaggagctt gcccttgccg ggcaccaccg tggtctcg
//
LOCUS       DAOSCR010000648         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54526_length_1545_cov_3.088591, whole genome shotgun sequence.
ACCESSION   DAOSCR010000648 DAOSCR010000000
VERSION     DAOSCR010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54526_length_1545_cov_3.088591"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1344
                     /locus_tag="PLT69_12425"
     CDS             262..1344
                     /locus_tag="PLT69_12425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ15290.1"
                     /translation="MLDLCVVTVLAVCLLATPALSSSAYVTVLLYHRFDENAFPTTTT
                     GSAQFERQLAYLRDHGYSVISLDHLARCVDKAAPFPERAVVITIDDGFISEYERAVPI
                     LKKYGYPFAIFVFTNGIGARGYMNWDQLRSVASMGGIVGSHTKSHPRLMNMSDQAIEK
                     EVMGSREILEKGLGRPVAYFAYPFGQYDERVRRIAHKAGFRLMLTSDPGSVGPGVERD
                     LVPRQAVVGMGMSLKDFARKLENPPLVVSGRDPGPGTLKGPTVPEVRLTIAEAHLYDP
                     GQVNVFLSEKGRIPFSFDPSTGALVCTGPFHLTRKTNRILVSARRKSDGLFALHSYLI
                     VLPGVWPDMRYTLLDRPTSALTGQDD"
     gene            complement(1332..>1545)
                     /locus_tag="PLT69_12430"
     CDS             complement(1332..>1545)
                     /locus_tag="PLT69_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15291.1"
                     /translation="LPGHHEPQRWAGPPRVQVSSRLVTGVTECPYVTARRLGNITNDH
                     GVSTAIQGSRPFRGRFSRAGRQVQSS"
BASE COUNT          268 a          500 c          517 g          260 t
ORIGIN      
        1 ctgaggcgga tacgtcgggc ctgcggcagg gcccacgacc cgtacgagct tgcccggggt
       61 ctcgagtgct actctgaaca agggcccctc tacgtggcca aggtcgccag ggtcataagc
      121 atgaatgacc ttcgcgagta cgacagttac aggctccggt agggtgaccc cgtcatgcaa
      181 cggcacgaag gaaggctgcc cgctcggggc ttggtgggcg acagcccctg ccggccgcgg
      241 cgttctggga ccgcccgagg cgtgctcgac ctgtgtgtgg tcacggtcct ggcggtgtgc
      301 ctcctggcga cgcccgcctt gtcgtcgagc gcctatgtga ccgtgcttct ctatcaccgt
      361 ttcgacgaga acgcctttcc caccacgacg accggctccg cacagttcga gagacagctc
      421 gcgtacctga gggaccatgg ttactcggtg atcagcctcg atcacctggc acggtgtgtc
      481 gacaaggcgg cgccctttcc cgagagggcc gtggtcatca ccatcgacga cgggttcata
      541 tccgagtacg agcgcgcagt cccgatcctc aaaaagtacg ggtacccctt cgcgatcttc
      601 gtcttcacca acgggatagg ggcgagggga tacatgaact gggaccagct caggtcggtc
      661 gcctccatgg gagggatcgt gggatcccac accaagagtc acccgcggct catgaacatg
      721 agcgaccagg ccatcgagaa agaggtcatg ggttccaggg agatcctgga aaagggtctc
      781 ggaaggcccg tggcctattt cgcgtacccg ttcggccagt acgacgagag ggtgaggcgc
      841 atcgcgcaca aggcgggatt caggctcatg ctgacgtccg acccggggag cgtggggccg
      901 ggcgtcgagc gtgacctcgt gccgaggcag gccgtggtgg gcatggggat gtccctcaag
      961 gatttcgccc gcaagctgga gaacccgcct ctcgtggtgt cgggccggga ccccgggccc
     1021 gggaccttga agggccccac cgtgcctgag gtgaggttga cgatagccga ggcacacctc
     1081 tacgacccgg gccaggtgaa cgtcttcctc tcggaaaagg ggcggatccc cttcagcttc
     1141 gacccgtcga ccggcgccct cgtatgcacg ggcccgtttc acctcacgcg caagacgaac
     1201 cggatcctcg tgtcggcgag gaggaagtcc gacggtctct tcgccctcca ctcgtacctc
     1261 atcgtgctgc ccggcgtctg gccggacatg aggtataccc tcctcgaccg gccgacttcg
     1321 gcgctgaccg gtcaagacga ctgaacctgt cggcccgccc gagagaagcg gcccctgaaa
     1381 ggcctcgatc cctggatggc cgtcgagacg ccatggtcgt tcgtgatgtt cccgagccgc
     1441 cgagccgtca cgtaagggca ctcggtgacg cccgtgacga ggcgggagga gacctgcacc
     1501 cggggcgggc cggcccaccg ctgcggctcg tgatgcccgg ggagg
//
LOCUS       DAOSCR010000649         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54574_length_1544_cov_3.098724, whole genome shotgun sequence.
ACCESSION   DAOSCR010000649 DAOSCR010000000
VERSION     DAOSCR010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54574_length_1544_cov_3.098724"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /gene="aroC"
                     /locus_tag="PLT69_12435"
     CDS             <1..1036
                     /gene="aroC"
                     /locus_tag="PLT69_12435"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOJ15292.1"
                     /translation="DADLKRRQQGYGRGGRMAVERDRVDVLAGLRGGLTLGSPVLLAV
                     WNADHENWKDLMDPWEIRPGREFTTPRPGHADLAGAARFRHKDLRNVLERSSARETAA
                     RVACGALLKSFLSELGVSAHAWVTRIGPVAFEGPFDRQARDASSVFCPDERATREMER
                     VIDEAAQAGDSVGGEFAVVVEGLPAGIGSSTQWDQRLDALLARHLMSIPAVKAVQVGA
                     GVTCGTLPGSLVHDPILPGSPKRRPTNNAGGMEGGMTNGEPVVLRCTMKPIPTLLKGM
                     PTVDIRDGRAVTSAYERSDVCAVPAASVVGEAAAVIAVAQAVCASFAQPSMEALTAAF
                     SEHRKYWEAL"
     gene            1033..>1544
                     /locus_tag="PLT69_12440"
     CDS             1033..>1544
                     /locus_tag="PLT69_12440"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ15293.1"
                     /translation="MIFLTGFMGSGKTTVGSRLAERLGYTFVDLDGLISDKAAGPIRE
                     IFAYAGEKAFRFLEREALAEVAAMPWSTVVATGGGAPVDASNRALMKACGTVVYLAAS
                     FETLRRRIPEDPERPLWGQDAQRLLEERKAAYEDADLVVNTDGLTVAEVVEELANLAP
                     GMVHPVPVPVP"
BASE COUNT          254 a          520 c          561 g          209 t
ORIGIN      
        1 cgacgccgac ctcaagaggc gccagcaggg gtacggcagg ggcgggagga tggccgtcga
       61 gcgtgaccgc gtggacgtgc tggccggact tcgcggcggc ctcacgctgg gcagcccggt
      121 gctcctggcc gtctggaacg ccgatcacga gaactggaaa gacctcatgg acccgtggga
      181 gatcaggccc ggcagggagt tcaccacgcc gagacccggc cacgcggacc tggcgggcgc
      241 cgccaggttc aggcacaaag acctcaggaa cgtcctcgag cgctcgagcg cgcgggagac
      301 cgcggcaagg gtggcatgcg gcgctctcct gaagtcgttc ctttccgaac tgggggtgag
      361 cgcgcacgcg tgggtgacga ggatcggccc cgtcgccttc gagggcccct tcgacaggca
      421 ggcgagggac gcatcgagcg tcttctgccc cgacgagcgg gcgacccgcg agatggagcg
      481 ggtcatagac gaggccgccc aggccgggga ttcggtgggg ggcgagttcg cggtcgtcgt
      541 agagggtctg cccgcaggca tcggctcgtc cacgcagtgg gaccagcgtc tcgacgccct
      601 gctcgccagg cacctcatgt ccatccccgc cgtcaaggcg gtgcaggtag gggccggcgt
      661 gacctgcggc accctgcctg gctcgctcgt ccacgacccc atcctgcccg gcagccccaa
      721 gaggaggccc acgaacaacg ccggcggcat ggagggcggg atgaccaacg gtgagcccgt
      781 agtcctgcgt tgcaccatga agcccatacc caccctactc aagggcatgc ccaccgtgga
      841 catacgggac ggccgggccg tcacgagcgc gtacgagcga tcggacgtct gcgcggtacc
      901 cgcggcctcg gtggtgggcg aggccgctgc ggtcatcgcc gtcgcccagg ccgtctgcgc
      961 gagcttcgcc cagccgagca tggaagcgct caccgcagcc ttctccgaac accggaagta
     1021 ctgggaggcg ttgtgatctt tctgaccggc ttcatgggct cgggcaagac caccgtgggc
     1081 tcgaggctcg cagagaggct cggctacacg ttcgtggacc tcgacgggct catcagcgac
     1141 aaggcggccg gacccatccg ggagatcttc gcctacgcgg gcgagaaggc gttcaggttc
     1201 ctggaacgcg aggccctcgc ggaggtcgcg gccatgccgt ggtccaccgt ggtcgcgacc
     1261 ggcgggggcg caccggtgga cgcgtccaac cgcgccctca tgaaggcgtg cgggacggtc
     1321 gtgtacctgg ccgcctcgtt cgagacgttg agacgccgca tacccgagga cccggaaagg
     1381 cccctgtggg gccaagacgc ccagagactc ctcgaggaga ggaaggcggc gtacgaggat
     1441 gcggacctcg tcgtgaacac cgacggcctg accgtggcag aggtggtcga agagctcgcg
     1501 aacctcgcgc ccggcatggt gcaccccgtg cccgtgcccg tgcc
//
LOCUS       DAOSCR010000650         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54828_length_1539_cov_3.144205, whole genome shotgun sequence.
ACCESSION   DAOSCR010000650 DAOSCR010000000
VERSION     DAOSCR010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54828_length_1539_cov_3.144205"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLT69_12445"
     CDS             <1..446
                     /locus_tag="PLT69_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ15294.1"
                     /translation="YLPSYGAEMRGGVTSCTVAISDEEIASPVASSPDYLVIMDNESL
                     MRLQSMAASGGEIYVNTSIVTGRPGRTDVEIVEIPATEIARGCTGEQYANMVMLGALV
                     CRTRQVRLETMIERMEEILGKAKARFKDTNIEALKAGYDYLSKEL"
     gene            451..>730
                     /locus_tag="PLT69_12450"
     CDS             451..>730
                     /locus_tag="PLT69_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15295.1"
                     /translation="MSVKQYRLQTANVSGKLSYLSDVLAKHGVNIRAISVGQESKDVS
                     SICVVVDDTKKAKNAFAVENIEFEEVDVLAVEMPDHPGGMNAVLKPLRD"
     assembly_gap    731..740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1002..>1539)
                     /locus_tag="PLT69_12455"
     CDS             complement(1002..>1539)
                     /locus_tag="PLT69_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15296.1"
                     /translation="VGRNIALGNLVESTALVQAMKIGLLNLPSTLPGPGTVIAWILMG
                     VEDFYNLDGIVSLVTAIALFAGHDAHDVEGLEELALSIVGRVYGIEDAGSDGSRKRTA
                     WRLFSRLVPRQYAVKGVNRWVKGFLRRLLRLRRKSRLLPMGLGVAASAWDAYNLVVKA
                     GMLAQAEATRMARVGARS"
BASE COUNT          317 a          488 c          474 g          250 t
ORIGIN      
        1 cctacctgcc ctcctacggt gcagagatgc gcggaggggt gacctcttgc accgtggcca
       61 tatccgacga ggagatagcc tcaccggtgg cctcttcccc ggattacctc gtgatcatgg
      121 acaacgagtc cctcatgagg ctccagagca tggccgcctc gggcggcgag atctacgtca
      181 acacgagcat cgtcaccggg aggccaggca ggaccgacgt ggagatcgtg gagatcccgg
      241 ccaccgagat cgctcgggga tgcacgggtg aacagtacgc gaacatggtc atgctcggcg
      301 ccttagtgtg caggacgcgg caggtgaggc tcgagaccat gatcgagcgc atggaggaga
      361 tcctcggaaa ggcgaaggct cgcttcaagg acacgaacat cgaggctttg aaggcggggt
      421 acgactacct gtcgaaggag ttatgaggac atgtccgtca aacagtaccg gctccagact
      481 gccaacgtat cgggaaagct ctcctacctc tccgatgtcc tcgcaaagca cggggtgaac
      541 atcagggcca tttccgtggg ccaggaatcg aaggacgtat cgtccatctg tgtcgtggtg
      601 gacgatacga agaaggcgaa gaacgccttc gcggtcgaga atatcgagtt cgaggaggtg
      661 gacgtcctgg cggtggagat gcccgaccat ccgggcggca tgaacgccgt gctcaagccc
      721 ctgcgtgacg nnnnnnnnnn caacgtgatc accatgtacc cctacatcgg gagggtcgcc
      781 aacccgatca tcatcatgga ggtggaccac ctcaagaccg cgacggacgt cctcaagagg
      841 aactgggtca agctctggga caaggagctc tatcggctct agcagggatc ttagccggat
      901 ccccggtgca cggtggaaaa cccggcaaac gcaacacctg gacgcccacg gtcacctgca
      961 cgaccccctc gagaccgcct ggtcggtggg tgacgttccc ctcatgatcg agccccgacc
     1021 cgcgccatgc gcgtcgcctc ggcctgggcg agcatccccg ccttgacgac gaggttgtag
     1081 gcgtcccatg cggatgcggc gacgcccagc cccatgggca ggagcctcga cttcctcctc
     1141 aggcgcaaaa gcctgcgaag gaagcccttc acccacctgt tgaccccctt cacggcatac
     1201 tgcctcggca ccaggcggga gaagagcctc cacgccgtcc tctttcgaga cccatcggac
     1261 cctgcatcct cgataccgta cacgcggccc acgatcgaga gcgccagctc ctccaggccc
     1321 tcgacgtcat gcgcgtcgtg acccgcaaac agggctatgg ccgtgaccag cgacacgatc
     1381 ccgtcgaggt tgtagaagtc ctcgaccccc atgaggatcc acgcgatcac ggtccccggg
     1441 cccggcaggg tggaggggag gttgaggagg ccgatcttca tggcctggac gagcgccgtg
     1501 gattcgacaa gattgcccag ggctatgttc ctgccaacg
//
LOCUS       DAOSCR010000651         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_54878_length_1538_cov_3.372893, whole genome shotgun sequence.
ACCESSION   DAOSCR010000651 DAOSCR010000000
VERSION     DAOSCR010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54878_length_1538_cov_3.372893"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..>1538)
                     /locus_tag="PLT69_12460"
     CDS             complement(357..>1538)
                     /locus_tag="PLT69_12460"
                     /inference="COORDINATES: protein motif:HMM:NF023809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOJ15297.1"
                     /translation="KDLDRSASIRELIHAGVTAFKIEGRLKDAAYVKNITAFYRRLID
                     SAISSESGLGKSSSGHCRTSFTPDPRKTFHRGGTPFFLREGDDDIACPITPKSTGEEV
                     GTVRSAGASWFTLEAGPGGIEAGDGICYVDPTGVVRGAKVVGRDGEKILVFGHAEGLV
                     PGVNIMRNHDHRFWRMLKSSTGVRHVEVSLSFGETPRGFRLRATDEDGITGEVVLDNP
                     KVPARDPEAAMATLRRQLGKAGGSMFSVRSIEIDSAAYFIPVSSINKMRRDVLAGLEE
                     ARISSHPREARRRVHGPLPPYPVTTLDYRFNVSNSLARRFYERHGVALIEDAFELKRP
                     RPPAVVMTSRHCIRRLLGACLRTKDGPRLPARLFIEHSGRTFALAFDCDACLMHVLTT
                     R"
BASE COUNT          257 a          493 c          507 g          281 t
ORIGIN      
        1 cctacgcggt cgacgcgggc gagctggcca ggaccaaggg cgagtacgtg gtcatgattt
       61 ccaacctgag caccaagtcc catgccgtgg gtacggtcgt catcacgtat ccggccggtc
      121 cgcaagcgag agagaaggtg gaaggccaga gacacaagag ggatgagtag aacgctcacg
      181 caccgccgtt gtcatgggta aaggcggtgc gtgttttttt gtgcgtgcgc gcctgtcgcc
      241 cgccgtcgag catgggcatg gaccagcccg ggacattccc ctcgtggcgg ctcctgccgg
      301 aacggagcgg gacgaggggg cgtttccctg cctgggaccg agtccgcctt ggcctgtcag
      361 cgcgtggtga gcacgtgcat gagacacgcg tcgcagtcga aggcgagcgc gaaggtcctg
      421 cccgagtgct cgatgaagag cctagcggga agtcgtgggc cgtctttcgt gcgcaggcac
      481 gcacccagaa gcctccggat gcaatgcctg gaggtcatga cgacggccgg cgggcgcggc
      541 cttttcagct cgaacgcgtc ctctatgagc gccacgccat gcctctcgta gaaacgcctt
      601 gcaagagagt tgctcacgtt gaacctgtag tcgagcgtgg tgacggggta gggtggcaac
      661 gggccgtgta ccctacggcg agcctcacgc gggtgggacg agatcctcgc ctcctccagc
      721 ccggcgagca cgtcgcgccg catcttgttt atcgaggaga cggggatgaa atacgccgcg
      781 gagtcgatct cgatcgacct cacgctgaac atgctcccgc cggcctttcc cagctgcctc
      841 ctcagcgtgg ccatggcggc ctccgggtcc ctggccggga ccttggggtt gtcgaggacg
      901 acctctccgg tgatcccgtc ctcgtccgtg gccctgagcc tgaacccccg cggtgtctcc
      961 ccgaaggaca gggacacctc gacgtgccta acgcccgtcg atgacttcag catgcgccaa
     1021 aagcgatggt cgtggttcct catgatgttc accccgggga cgagtccttc ggcatggccg
     1081 aacacgagga tcttctcccc gtccctgccc accaccttgg cgcccctgac gacccctgtg
     1141 gggtcgacat agcagatccc gtctcctgcc tcgatcccgc cgggcccggc ctcgagggtg
     1201 aaccatgagg cgcccgcaga cctcacggtg cccacctcct cgcccgtgga cttcggggtg
     1261 atgggacagg cgatgtcgtc gtcgccttct ctcaggaaga acggggtgcc gccgcggtgg
     1321 aaggtcttgc ggggatcggg ggtgaagctc gtgcggcaat gcccgctgga agacttcccg
     1381 aggccgcttt cagaagagat cgccgaatct atcaggcgcc ggtagaaggc ggtgatgttc
     1441 ttcacgtatg ctgcatcctt gagccttccc tcgatcttga aggccgtcac cccggcatgg
     1501 atgagctccc tgatggaggc gctccggtcg agatcctt
//
LOCUS       DAOSCR010000652         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_55070_length_1535_cov_1.986486, whole genome shotgun sequence.
ACCESSION   DAOSCR010000652 DAOSCR010000000
VERSION     DAOSCR010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55070_length_1535_cov_1.986486"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1535
                     /gene="mnmG"
                     /locus_tag="PLT69_12465"
     CDS             <1..>1535
                     /gene="mnmG"
                     /locus_tag="PLT69_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOJ15298.1"
                     /translation="ETAISYRVLNTKKGPAVRATRTQNDRRLYEAAVRRRVESAGIHL
                     RQVRVDSLVLDGTRITGVKDRFGVLYRARAVVITAGTFLQGLIHIGDTRQKAGRAGEE
                     ASYELAESLEALGFEMGRFKTGTPPRLDVHTIDLSALQRQDSQEGCMPLGFDSRGPHL
                     PQVPCYLARTSPATHELVRRHIGKSPLYSGAITGTPARYCPSLEDKVMRFGDRDGHQV
                     VIEPEGLDTREVYASGLGNSMPVEIQWEIVRSVPGLERAEIVRPAYAIEYAYVKPTQL
                     MRTLETKRVEGLYLAGQVNGTSGYEEAAGQGLVAGVNAALKIKNEPPFVLDRSTAYIA
                     VMIDDLVTKGVSEPYRLFTSRAEHRLVLRETNAVFRLSGEALKIGLIGRDRFERVQAV
                     EAEVSSLREHLRKTTVTVPRDLPGALSPGNDDAPRTTLERLLKRPGITMEHLRDRGLV
                     PVSSELAGIEAEIGIKYEGYIARQLEETRRLRDMEGVEIPPTFDYAAVPGLSNELRCR
                     LDA"
BASE COUNT          291 a          514 c          518 g          212 t
ORIGIN      
        1 cgagaccgcg atctcgtacc gtgtgctcaa caccaagaag ggaccggcgg tgcgcgccac
       61 caggacccag aacgacagga ggctctacga ggcggcggtg aggaggcgcg tggagtccgc
      121 gggcatccac ctcaggcagg tgcgggtgga ttccctcgtt ctcgacggga ctcgcataac
      181 cggcgtcaaa gaccgcttcg gggtcctgta cagggcccgc gcggtcgtga tcaccgccgg
      241 gacgttcctc cagggcctca tccacatcgg cgacacccga cagaaggcgg gcagggccgg
      301 ggaggaggcc tcctacgagc tggcggagag cctcgaggcc cttgggttcg agatggggcg
      361 gttcaagacc gggaccccgc cgcgcctcga cgtgcacacc atcgatctct cggcgctgca
      421 acggcaggat tcacaggagg gctgcatgcc gctggggttc gactccaggg ggccgcatct
      481 cccccaggtc ccctgctacc tggcgaggac ctcgcccgcc acccacgagc tcgtcaggag
      541 gcacatcggc aaaagcccgc tctactccgg cgcgatcacc ggcacacccg ccaggtactg
      601 cccgtcgctc gaggacaagg tgatgcgttt cggtgacagg gacggccacc aggtcgtcat
      661 cgaacccgag ggtctcgaca cccgggaggt ctacgcatcc gggctcggaa acagcatgcc
      721 cgtggagatc cagtgggaga tcgtccgctc ggtccccggg ctcgagcggg cggagatcgt
      781 gcgacccgca tacgccatag agtacgccta cgtgaaaccc acccagctca tgaggacgct
      841 cgagaccaag agggtcgaag ggctgtacct cgcgggacag gtcaacggca cctccggcta
      901 cgaggaggcg gccggccagg gactggtggc cggtgtcaac gcggcgctca agatcaagaa
      961 tgagcccccg ttcgtcctcg accgttcgac ggcctacatc gcggtcatga tcgacgacct
     1021 cgtcacgaaa ggcgtgagcg agccctacag gctcttcacc tccagggccg agcacaggct
     1081 cgtgctcagg gagaccaacg cggtcttccg gctgagcggc gaggcgctca agatcggcct
     1141 catagggaga gaccggttcg agcgcgtcca ggccgtggag gcggaggtct cgtccctgag
     1201 ggaacacctc cggaagacga cggtgaccgt gcccagggac ctgcccgggg cgctctcccc
     1261 cggcaatgac gacgccccgc gcacgacgct cgagaggctc ttgaagagac ccgggatcac
     1321 catggaacac ctcagggaca gaggcctcgt ccctgtctcg agtgagctag ccggtatcga
     1381 ggcggaaatc gggatcaagt acgaggggta catcgcccgc cagctcgagg agaccaggcg
     1441 cctcagggat atggagggcg tcgagatccc cccgacgttc gactacgccg ccgtccccgg
     1501 gctctccaac gagctcaggt gcaggctcga cgccg
//
LOCUS       DAOSCR010000653         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_55154_length_1533_cov_3.303112, whole genome shotgun sequence.
ACCESSION   DAOSCR010000653 DAOSCR010000000
VERSION     DAOSCR010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55154_length_1533_cov_3.303112"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            733..1410
                     /locus_tag="PLT69_12470"
     CDS             733..1410
                     /locus_tag="PLT69_12470"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ15299.1"
                     /translation="MGVSKSMRRILTSSAFHDVLYRFVRAYSATFSLSVTNEKAWMEH
                     VAAGGRVILCTWHQQFFTAISHFKNYERFRPSLMISKSMDGSIIAGVARRCGWHTVAG
                     SSSKGGAEALKEMIGSVRKNGLAGHVVDGPRGPAGRVKPGLIRMAHEAEACVVPFFTE
                     ASSAWFFDSWDNFMLPKPFARVRLVFLDMMSLAPTTDRDEFERQRLAVEEAMRPHLKG
                     RLSGPGA"
BASE COUNT          265 a          490 c          540 g          238 t
ORIGIN      
        1 cccgcacggc aggacgcctg ccgaggggca cggcctaccc ccgcagacga tccctcgagc
       61 tgcatggggc cgggagcggg ctaggtgcct gtgctgtcgg tgtcatgggg gacatccagc
      121 agacgatccc tcaagctgaa ttgcccggga ggccgcccct tcgccgcctg agcccgagcg
      181 tgcagggcac gtcaagggcc cggtcccgcg ccgcccacgg ccctaagcac cgacccggtt
      241 gctctgacag ggtcgggcag gcgggaaacg agtcggcggt gaacggacga gaacgttggt
      301 cgtgtcgcat ggtccacgcg ccgcccttgc atgagggggc atccctcccg aaagaagtgc
      361 cggaccgttc tcgatggacc cacgagacac ccgggcctgg ccccgggcct cgccctggga
      421 gaaggcgggc gagaggcccc ggccaccggg ttcggccgtg acccaggcaa gaggagggca
      481 tgccgggggt acatcgagga aaggcctggc gcgagcgggg gcggtggggt gcggacggcg
      541 gaagagatcg agtgtgcccg tgcactgtga gaccggggcc accgtggacg gtcaaagaac
      601 tgtgtcaggg tgctttctga ccctttcccc ctttgacttc cacgaggcat tcgatcatag
      661 atggcaagac gagggtccgc gagggtggtc ccctcgcgtc acccagcgtc cccttgcatg
      721 gaggtgaaca ccttgggtgt ctccaagtcg atgagacgca tactcacgtc gagcgccttc
      781 cacgacgttc tctaccggtt cgtccgcgcc tacagcgcca cgttcagcct gtcggtgacg
      841 aacgagaagg cctggatgga acacgtggcc gcaggcggca gggtcatcct gtgcacgtgg
      901 caccagcagt tcttcacggc catctcccac ttcaagaact acgaacgctt caggccttcc
      961 ctcatgatca gcaagagcat ggacggttcc atcatcgcgg gcgtggcgag aaggtgcggg
     1021 tggcacacgg tggccgggtc gtcctccaaa ggcggcgccg aggcgctgaa ggagatgatc
     1081 gggtctgtca ggaagaacgg cctggcaggc cacgtggtgg acggcccacg cggccctgca
     1141 ggcagggtca agccggggct catccggatg gcccacgagg cggaggcctg cgtcgtaccc
     1201 ttcttcaccg aggcctcgag cgcctggttc ttcgacagct gggacaactt catgctgccc
     1261 aagccgtttg cgcgtgtgcg gctggtcttt ctggacatga tgagcctcgc gcccacgacg
     1321 gaccgtgatg agttcgagag gcagcgcctg gccgtcgagg aggccatgag gccgcacctg
     1381 aaaggcaggc tctcggggcc gggtgcgtag ctcctcatca aggcgcggcc gttgcagcaa
     1441 ggtgggctaa acggcgcgga gatcagcgcg gaccttcagc gcacaagccg caccccttgc
     1501 cgggtgaagg cattcccggt gggcgcgccg ccc
//
LOCUS       DAOSCR010000654         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_55195_length_1532_cov_5.868653, whole genome shotgun sequence.
ACCESSION   DAOSCR010000654 DAOSCR010000000
VERSION     DAOSCR010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55195_length_1532_cov_5.868653"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..433)
                     /locus_tag="PLT69_12475"
     CDS             complement(152..433)
                     /locus_tag="PLT69_12475"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOJ15300.1"
                     /translation="MYETVTIQILGQEYRVRSGEEGDHVRSLSRYINEKVSDIQKSGK
                     AVTTAELVAMTMLHMADDVAKARKELEILRETVSDRISRIIDRIAAASP"
     gene            complement(438..650)
                     /gene="zapB"
                     /locus_tag="PLT69_12480"
     CDS             complement(438..650)
                     /gene="zapB"
                     /locus_tag="PLT69_12480"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HOJ15301.1"
                     /translation="MEILDILEQKVCELVETVNELRRKNEELLIMLKEKEEEAQGLRR
                     EMDHLLGEKEKVRQKVAHLIRCVEAL"
     gene            871..1362
                     /locus_tag="PLT69_12485"
     CDS             871..1362
                     /locus_tag="PLT69_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ15302.1"
                     /translation="MEGPYLETLYELVRRLNPSPDITDVFETVVNRLPHVLDTKYCSL
                     FIINPASGELELRAHNHPDIGENPFISVGKQRESIMNLALERGSSLIIKDIEEEIGLP
                     KKDKYETKSFMCILIRRNEEVLGVLNLADKASGGFSREDMLFATIVCELLGAILGPGQ
                     ALS"
     gene            1373..>1532
                     /locus_tag="PLT69_12490"
     CDS             1373..>1532
                     /locus_tag="PLT69_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ15303.1"
                     /translation="MSFTCGIVGLPNAGKSTLFNLVTKAGAPAENYPFCTIDPNIGVV
                     NVPDENLEA"
BASE COUNT          301 a          481 c          416 g          334 t
ORIGIN      
        1 aacgttcacg accagggaac acaaccctcg agagccttca gcgtcacccg cctctcagcg
       61 gcgggatcgt gggaatgtgg atgcgggcct gggccccctg ttcgtctgta cccgggatcg
      121 ggatgtcacg ctcgggccgg ggaggcgcct ctcacggcga tgcggccgct atccgatcga
      181 tgatcctgga tatcctgtcg gatacggtct ccctgaggat ctccagttct ttacgggcct
      241 tggccacatc gtccgccatg tggagcatgg tcatggccac cagttcagcc gtggtcacgg
      301 ccttgcccga cttctggatg tccgaaacct tttcgttgat atacctcgag agggacctga
      361 cgtggtcccc ttcttccccg gatcgcaccc ggtattcctg cccgagtatc tgtatggtta
      421 ccgtctcgta caaggtctta cagtgcttcc acgcacctta taaggtgtgc caccttttgt
      481 ctgacctttt ctttctcacc caagagatga tccatctctc gcctcaagcc ttgcgcttct
      541 tcctcctttt ctttgagcat tatgagcaat tcttcgttct ttctcctcaa ttcgttgacc
      601 gtctcgacga gctcgcatac cttctgttcc agaatgtcga gtatctccaa gagagagtct
      661 ccttccaccg ttggaaaaga attatacatc catcgtgccc agggaagtca aggcccgcgt
      721 aacaaggtgg gttttcacga atcaccgccg caaggtaccc ttcgagcgtg cacagggtca
      781 ccatgcatcg cgatcgatgc cgtggtgaaa ttcaccaatg acctctcggc ggccgatatg
      841 atagaatcat gtcgtcaggg agggttccgc atggaaggtc cttacctcga aacgctttac
      901 gagctggtca ggcggctcaa cccgtcgccc gacatcaccg atgtcttcga gaccgtggtg
      961 aaccggctgc cccacgtgct ggacacgaaa tactgctcgc tgttcatcat caaccccgcc
     1021 tcgggcgagc tcgagctcag ggcgcacaac caccccgaca tcggcgagaa ccccttcatc
     1081 tcggtgggaa agcagaggga gagcatcatg aacctggccc tcgaacgggg cagttcgctc
     1141 atcatcaagg acatcgagga ggagatcggg ctgcccaaga aggacaagta cgagaccaag
     1201 tccttcatgt gcatcctcat acgtcgcaac gaagaggtgc tcggggtgct gaacctcgcc
     1261 gacaaggcct cgggtggttt ctcccgcgag gacatgctct tcgcgacgat cgtctgcgag
     1321 ctcctgggag cgatcctggg cccgggccaa gccctttcct gagaggggcg ccgtgagctt
     1381 cacctgcgga atcgtggggt tgccgaacgc agggaagtcg acgctcttca acctcgtcac
     1441 gaaggccggc gcccctgccg agaactaccc cttctgcacc atcgacccca acataggcgt
     1501 cgtgaacgtc ccggacgaga acctcgaggc gt
//
LOCUS       DAOSCR010000655         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_55854_length_1519_cov_2.727459, whole genome shotgun sequence.
ACCESSION   DAOSCR010000655 DAOSCR010000000
VERSION     DAOSCR010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55854_length_1519_cov_2.727459"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(889..>1519)
                     /locus_tag="PLT69_12495"
     CDS             complement(889..>1519)
                     /locus_tag="PLT69_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ15304.1"
                     /translation="EKLGAKKIGFLYQNDAYGKDGLAGCKQRLKAYNMTLVEEVPVEP
                     SEKDLSSQLLRLKNSGAEVVFMWVNPTTAVITLKTSATIGFKPQWFSSDTLSDYPLMD
                     TLTGGLWEGVITGAFGMPPLSDHPLMVKYREAVKRFAAYAKERYRDCVDIDGVRFTDK
                     DAWGLVRPSNTQPVIVLRFEAKNSHELSAIEGDTRKRLEEIVRELSGGR"
BASE COUNT          266 a          504 c          490 g          259 t
ORIGIN      
        1 ccaaacaggc gagaaagatg ggcctggcgc tccatggtct cattgcatgc cccccatacc
       61 ctgtctgtcg cgtcctgtgt gcaacctatc gtaaatacgc gatgcacgca accccttcga
      121 cgctggaggc cggcgcgcat ccttgggctc gccgcgaagg cggtgggaga cgtcggtccc
      181 gcgcagacag gcccgcccgg ctgggttctc caggggcctt ttccccagag agggtttccg
      241 gccgggcctg ctttccgccg gcttgcggag ggggcaggaa gaacgggcac agggacccgg
      301 gcgggcatgg gccatgcgca ccgcctgcgc cgcatccggg tgagcgagaa aagggacccg
      361 ggcgggcata agccatgcgc cgactgcccc aggctggtga ccgccagcga caagggaccc
      421 gatcgggcat gggccatgcc cttgccccgc atgcctgacc cacgggcgcc acggttgttc
      481 tcccggctca cgccgcaccg tggaagaccg gggacgaggt ctccccggcg agagaagaga
      541 aggcgggacc ttcccggggg gttgaccccc tgccctaaga cccgacaggg aactctacaa
      601 ggggcaccga gacgccccgg cgggcgcaag ggacgaccac cggccccggc gctcggagat
      661 gtgatcgact ctcgcacccg gtctcgtggg ggcacaaccg tgccctgcgt gcgcaggagt
      721 tccaggcagc ctttcactca cgggatcggg gccgacaaac gggggtcaag gacgaacacg
      781 gagccacacc cgccgcaacg ggcgcgagac gtgcacggtg actgccgttt ctctataccc
      841 gagcgctgaa cttaaaacct gcgtgcgagc cgggggccga ccccgcgttc accgaccgcc
      901 cgagagctct ctcacgattt cctcgagcct cttcctcgta tcaccctcga tggcactcaa
      961 ttcatggctg tttttcgcct cgaagcgcag cacgatcacg ggctgggtgt tcgaaggtct
     1021 cacgagcccc cacgcgtctt tatcggtgaa cctgacgccg tcaatgtcca cgcagtctcg
     1081 gtagcgttct ttcgcgtagg ccgcgaaacg cttgaccgcc tcgcgatact tgaccatcag
     1141 cgggtgatcg gacagggggg gcatgccgaa cgccccggtg atcacgcctt cccagaggcc
     1201 ccctgtcagg gtgtccatga gagggtagtc ggacagggtg tcggaggaga accactgggg
     1261 cttgaagccg atcgtggcgc tggttttgag cgtgatgact gcggtggtgg ggttcaccca
     1321 cataaacacg acctcggcgc ccgagttctt aagcctgagc agctgggatg acaggtcctt
     1381 ctcgctgggc tccaccggaa cctcttcgac cagggtcatg ttataggcct tgagccgctg
     1441 cttgcagcct gcaagcccat ccttgccgta cgcgtcgttc tggtagagga acccgatctt
     1501 ctttgcaccc agcttctcg
//
LOCUS       DAOSCR010000656         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_56058_length_1515_cov_2.820548, whole genome shotgun sequence.
ACCESSION   DAOSCR010000656 DAOSCR010000000
VERSION     DAOSCR010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56058_length_1515_cov_2.820548"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /gene="dprA"
                     /locus_tag="PLT69_12500"
     CDS             <1..982
                     /gene="dprA"
                     /locus_tag="PLT69_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ15305.1"
                     /translation="AAETELRSTEFLRPSQIRSILVGPDEARVDEVVRALEKVRARAV
                     SLMDREYPAALRAIDAPPAVLYVRGSLEDIEPAVAVVGTRSPSVYGKRTAFFLARDLS
                     LSGVSVVSGLARGIDREAHLGALEGIAKTVAVLGSGIDTVYPKEHEDLASRIERAGAV
                     VSELPPGTKPEAGNFPRRNRIISALAQAVVVVEATTRSGAMITARLGLEQGKVVMAVP
                     GNVTNLRSQGPHHLIRQGAVLVERAADVIAEIAPSIASTLSRQPLKQPGSRTDEILNM
                     ASSSPVTIEEIAQHLGIDVSEATRRVSTLEIEGSLERVTPNRFIARDVHG"
     gene            975..>1515
                     /locus_tag="PLT69_12505"
     CDS             975..>1515
                     /locus_tag="PLT69_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOJ15306.1"
                     /translation="MAKTLLIVESPAKARTIKRYLGNRFDVEATMGHIKDLPKSTMGI
                     DLENGFIPSYRIIADKKDVVKKIKARAKSADLVYLASDPDREGEAIAWHVAEEIGKPP
                     ESVRRITFHEITKRAVEEAIKSPKELNRSLYDAQMARRSMDRIVGYTMSPLLWKKVRR
                     GLSAGRVQSVALRLVCMRQE"
BASE COUNT          294 a          481 c          521 g          219 t
ORIGIN      
        1 ggccgccgag acggaactgc gctccacgga gttcctcagg ccctcgcaga tcaggtcgat
       61 ccttgtggga ccggacgagg cgcgcgtcga cgaggtggtg cgggcgctcg agaaggtgcg
      121 cgcacgggcg gtatccctca tggaccgcga gtatccggcc gccctgaggg cgatagatgc
      181 cccccccgcg gtcttgtacg tcaggggtag tctcgaggac atcgagccgg ccgttgcggt
      241 ggtgggtacc cgttcaccct cggtgtacgg aaagcgcacg gccttcttcc tcgccaggga
      301 cctgagcctg agcggcgtct cggtggtctc gggcctcgcc agggggatcg accgggaggc
      361 ccatctgggt gcgctcgaag ggatagcaaa gacggtggcc gtgctgggct ccggcatcga
      421 taccgtctac ccgaaggagc acgaggatct cgcctcgagg atcgagcggg caggcgccgt
      481 cgtctcggag ttgcccccgg gcacgaagcc ggaggccggg aacttcccca ggaggaaccg
      541 catcatctcc gccctggccc aggccgttgt cgtggtcgag gcgacgacga ggtccggggc
      601 catgatcacc gcgcgcctcg gcctcgagca ggggaaggtg gtcatggccg tgcccgggaa
      661 cgtgacgaac ctgaggtcgc aggggcccca ccacctcata cggcaggggg cggtcctcgt
      721 cgagagggcc gcagatgtca tcgccgagat agcaccctcc atcgcctcga ccctgtcccg
      781 ccagccactc aagcaacctg gttccaggac cgatgaaatc ctcaacatgg catcgtcctc
      841 gccggtcacc atagaagaga tcgcacaaca cctgggcata gacgtgagcg aggcgacccg
      901 gagggtgagc acgctcgaga tcgaggggtc gctcgagagg gtgacaccga accgcttcat
      961 cgcgagggac gttcatggct aagacgctgc tcatcgtgga gtcgcccgca aaggcacgca
     1021 cgatcaaacg ctacctggga aacaggttcg acgtggaagc caccatgggc cacatcaagg
     1081 acctgccgaa gagcaccatg ggcatagacc tggagaacgg cttcatcccg tcgtacagga
     1141 tcatcgcgga caagaaggac gtggtgaaga agatcaaggc ccgggcgaag tccgcggacc
     1201 tggtctacct cgcgtcggac cccgaccggg agggcgaggc catcgcctgg cacgtcgcgg
     1261 aggagatcgg caaacccccg gaatccgtcc gccgcatcac cttccacgag atcaccaaga
     1321 gggccgtgga ggaggccatc aagagcccga aggagctcaa ccggtccctc tacgacgcgc
     1381 agatggcgag acgctccatg gaccggatcg tggggtacac catgagcccg ctcctctgga
     1441 agaaggtccg cagggggctg tcggcgggca gggtgcaatc cgttgccctg aggctcgtgt
     1501 gcatgcgcca ggaag
//
LOCUS       DAOSCR010000657         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_56880_length_1500_cov_3.414533, whole genome shotgun sequence.
ACCESSION   DAOSCR010000657 DAOSCR010000000
VERSION     DAOSCR010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56880_length_1500_cov_3.414533"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLT69_12510"
     CDS             <1..351
                     /locus_tag="PLT69_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOJ15307.1"
                     /translation="LEEIDADLAESIRQKMFVFEDLINIDNRGIQAILKEITNEDLSL
                     ALKTASEGLKSHILKNMSSRAAEMLLEDMEVMGPVKLSDVEMAQQNIVRVARKLEASG
                     KIVIGGKGGEDVLV"
     gene            338..982
                     /locus_tag="PLT69_12515"
     CDS             338..982
                     /locus_tag="PLT69_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOJ15308.1"
                     /translation="MSSSKIIRDDDPKSCTVSPYRIREMDAKRVVQDAAQQAEEIIKK
                     ARAEKEAIEMEAYRKGLEQGQAQGQMMAVKRVEPLLEALSKAVREIKDLRQSIVETHK
                     DQVVELLVLMTERIVHRIVTLDPDIVLETVKQACNHVAEADEIRIRLHPSDFEYIRDI
                     ERIMAQNLTTTNGLVFVEDSTVGRGGVILETAFGEIDATIRSQIDHMREVLLEK"
     gene            979..>1500
                     /gene="fliI"
                     /locus_tag="PLT69_12520"
     CDS             979..>1500
                     /gene="fliI"
                     /locus_tag="PLT69_12520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HOJ15309.1"
                     /translation="MTDLSRQILQIRELDPIVVRGKVTNIVGLVVEGHGPGSAMGGMC
                     EIFPRSKGESVMAEVVGFRDKRVLLMPLGDLNGIGPGSLIVARRSQPTVKVGHGLLGR
                     VIGGMGEPLDGKGPHVLEHELPLYGNPFNPLRKRRIEKPLELGIRSIDGLLTVGKGQR
                     MAIMAGSGVGKSVL"
BASE COUNT          325 a          456 c          503 g          216 t
ORIGIN      
        1 ctcgaggaga tcgacgcgga ccttgcggag agcatacgcc agaagatgtt cgtgttcgag
       61 gacctcatca acatcgacaa caggggcatc caggccatcc tcaaggagat cacgaacgag
      121 gacctgtctc ttgccctcaa gaccgcgagc gaggggctca agtcccacat cctcaagaac
      181 atgtcctcga gggcggcgga gatgctcctg gaggacatgg aggtcatggg cccggtcaag
      241 ctgtcggacg tggagatggc ccagcagaac atcgtgcgcg tggcgaggaa actggaggcg
      301 tccggcaaga tcgtgatcgg cggaaagggc ggagaggatg tcctcgtcta agatcatcag
      361 ggacgacgac ccgaagagct gcaccgtctc gccgtaccgc atacgcgaga tggacgcgaa
      421 gagggtcgtg caggacgcgg cccagcaggc cgaggagatc atcaagaagg cccgcgcgga
      481 gaaggaagcc atagagatgg aggcctaccg caagggcctg gaacagggtc aggcccaggg
      541 gcagatgatg gccgtcaaac gggtcgagcc cctgctcgag gccctgtcca aggccgtgag
      601 ggagatcaag gacctgcgcc agtccatcgt ggagacacac aaggaccagg tcgtcgagct
      661 cctcgtcctc atgaccgaac ggatcgtgca ccgcatcgtc accctcgacc cggacatcgt
      721 gctcgagacg gtgaagcagg cgtgcaacca cgtcgccgag gccgacgaga tacgcatacg
      781 gcttcaccct tcggacttcg agtacatccg cgacatcgag cgcatcatgg cccagaacct
      841 cacgacgacc aacggcctgg tcttcgtcga ggactccacg gtcggccgtg gcggcgtgat
      901 cctggagacg gcgttcggtg agatcgacgc caccatacgg tcccagatcg accacatgcg
      961 ggaggtactc ctggagaaat gaccgacctg agccgccaga tcctccagat cagggaactc
     1021 gaccccatcg tggtcagggg caaggtcacg aacatcgtcg gcctcgtcgt cgagggccac
     1081 ggcccgggca gcgcgatggg cgggatgtgc gagatcttcc cgagatccaa aggggagtcc
     1141 gtcatggcgg aggtcgtggg tttcagggac aagcgcgtgc tcctcatgcc gctgggtgac
     1201 ttgaacggca tcgggccggg aagcctcatc gtggcgagaa ggtcccagcc cacggtgaag
     1261 gtgggccacg gcctgctggg ccgcgtgata ggcggcatgg gcgaaccact ggacgggaag
     1321 ggcccgcatg tcctcgagca cgagctcccc ctgtacggca accccttcaa cccgctgagg
     1381 aaaaggcgca tcgagaaacc cctcgaactc ggcatacgct ccatcgacgg cctgctcacc
     1441 gtgggcaagg gccagcgcat ggccatcatg gcgggctcgg gcgtcggcaa gagcgtgctc
//
LOCUS       DAOSCR010000658         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_57448_length_1491_cov_3.216574, whole genome shotgun sequence.
ACCESSION   DAOSCR010000658 DAOSCR010000000
VERSION     DAOSCR010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57448_length_1491_cov_3.216574"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1491)
                     /locus_tag="PLT69_12525"
     CDS             complement(<1..>1491)
                     /locus_tag="PLT69_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOJ15310.1"
                     /translation="AGPAAIMGAMGYRRRTGFLAGLTVAQISEFSLIVVASGLALGHI
                     GQEIVGLVTVVGVVTILASTYMILYSDRLYQALSRWLRAFERANPYREAQCNDCFIMP
                     RVDVVLVGLGGYGRELAEHLLERGRHVIGVDFDPEALEYCAKRGIPVLYGDMADPETI
                     DGLPFLQARWVVSTTRNPAVDVQIVRHLRAKGFGGRIALTARTKEDAEAYLHFKPDVV
                     LRPFFDAAEQGADALTGAWHALSDKTGWPVSFVEIRVKAGSTFAGHAIRDIPLRSELG
                     VAIAAVSRAGRVFFNPEPDLRIFPSDRVVLMGPPEAVKRAETMIHRLADAPGEDDGSA
                     VSLVEVQVGPGSPQAGKTLGETGFRRVTGPSSWGSGGATSTSSPPGPTNACSQETRCS
                     CSEPRMHSSASASASSARPETRPGHESLRVPSDTTRRGCLSRCQEGRRRAVMPPRPSV
                     GWRPGFGGDEGRPLSCVAGVFRAGGPMPLVAVSATRGLSCACSAGVR"
BASE COUNT          235 a          541 c          503 g          212 t
ORIGIN      
        1 cgcacacccg ccgagcatgc acaagacagc ccgcgggttg ccgaaacggc gaccaggggc
       61 atgggacccc cggcgcggaa aacacccgcc acgcatgata ggggccggcc ttcgtcgcct
      121 ccgaagcccg gtctccagcc caccgagggc cgaggcggca tgaccgcacg gcgtcggcct
      181 tcctgacacc tggagaggca gccccggcgg gtggtgtccg aggggacccg cagggactca
      241 tgccccggcc gagtctccgg ccgcgcgctc gaagccgagg ccgaggcgct cgagtgcatc
      301 cgcggttccg agcacgagca gcgtgtctcc tgcgagcacg cgttcgtcgg gcccggggga
      361 gatgacgtac tcgtcgcccc gcctgatccc cacgacgagg gccccgtaac ccggcgaaac
      421 cccgtctccc cgagcgtctt tcccgcctgc ggcgagccag ggcccacctg cacctcgacg
      481 agggagacgg ccgagccgtc gtcttcgccc ggcgcgtcgg cgagcctgtg gatcatggtc
      541 tcggcgcgct tgacggcctc gggcggcccc atgagcacga cgcggtccga ggggaagatc
      601 ctcaggtcgg gctcggggtt gaagaagacc cttcccgccc tgctcaccgc cgcaatggcc
      661 acgccgagct cggacctgag cgggatgtcc ctgatcgcgt gcccggcgaa ggtgctgccc
      721 gccttgacgc ggatctcgac gaaggagacc ggccagccgg tcttgtcgga gagcgcgtgc
      781 caggcgccgg tcagcgcgtc cgccccctgc tccgcggcgt cgaagaacgg cctgagcacc
      841 acgtcaggct tgaagtgcag gtatgcctcg gcgtcctcct tcgtgcgcgc ggtcagcgcg
      901 attctccccc cgaagccctt tgccctcagg tgccgtacga tctgcacgtc caccgcgggg
      961 ttgcgcgtgg tgctcacgac ccagcgggcc tggaggaacg ggaggccgtc gatggtctcg
     1021 gggtccgcca tgtcgccgta gaggaccggg atacccctct tcgcgcagta ctccagggcc
     1081 tcggggtcga aatccacgcc tatgacatgc ctgccccgct ccagcaggtg ctcggcaagc
     1141 tccctgccgt atccgccgag ccccacgaga accacgtcca ccctcggcat gatgaagcaa
     1201 tcgttacact gcgcctctcg gtagggattg gcgcgctcga acgccctgag ccacctcgag
     1261 agcgcctggt agaggcggtc ggagtagagg atcatgtagg tggatgcgag gatggtcacc
     1321 acgcccacca ccgtcacgag accgactatc tcctggccga tgtgcccgag ggcgagcccg
     1381 gatgcgacca cgatcaggga gaactcgctg atctgcgcca cggtgaggcc ggcgaggaac
     1441 cccgtcctcc tccggtagcc catggcgccc atgatggcgg ccggcccggc g
//
LOCUS       DAOSCR010000659         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_57605_length_1489_cov_2.021618, whole genome shotgun sequence.
ACCESSION   DAOSCR010000659 DAOSCR010000000
VERSION     DAOSCR010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57605_length_1489_cov_2.021618"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /gene="gspE"
                     /locus_tag="PLT69_12530"
     CDS             <1..1187
                     /gene="gspE"
                     /locus_tag="PLT69_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOJ15311.1"
                     /translation="DITAMETEAPVIRLVNRLMVQAYRERASDIHVEPYPNDVKVRFR
                     VDGILHDVLSLPKRLHPALVSRIKVMASLNIAEKRLPQDGRIGVKLGDHSVDLRISTV
                     PTVNGERVVMRILDKSSVLLGLEELGFYPDDLATVERLIRQEHGIILVTGPTGSGKTT
                     SLYSMLSRINSPDKNILTIEDPIEYQLKGIGQIPVNVKVGLTFASGLRSIVRQDPDVI
                     LVGEIRDLETAEIAIQAALTGHLVFSTLHTNDSASAVTRLIDMGIEPFLVTSSVNAIV
                     AQRLTRKICSHCRQPYHPESETLLEVGLSRELLGSEGYLWRGAGCKECLGTGYKGRTG
                     IYEILLMSDSIKSTILKTSDSNIIKKQAVSEGLHSLREDGARKVKDGITTIEEVLRVT
                     QQ"
BASE COUNT          282 a          506 c          474 g          227 t
ORIGIN      
        1 aggacatcac cgccatggag acggaggccc cggtcatccg cctcgtgaac aggctcatgg
       61 tgcaggcata ccgggagcgg gcctcggaca tccacgtgga gccgtacccc aacgacgtga
      121 aggtccgttt ccgggtcgac gggatcctcc acgacgtgtt gagcctcccc aagaggctcc
      181 accctgcgct cgtgtcgcgc atcaaggtca tggcatccct caacatcgcc gagaagcgcc
      241 tcccccagga cgggaggatc ggcgtgaagc ttggcgacca ttccgtcgac ctgaggatct
      301 ccaccgtgcc cacggtgaac ggcgaacgcg tggtcatgag gatcctcgac aagtcgtcgg
      361 tgctgttggg gctggaggag ctgggattct accccgacga tctcgccacg gtggagcggc
      421 tcatacgcca ggagcacggt atcatcctgg tgaccgggcc gaccggctcg ggcaagacga
      481 cgtccctcta ctccatgctc agcaggatca actcgccgga caagaatatc ctcaccatcg
      541 aggaccccat cgagtaccag ctcaagggca tcggccagat acccgtcaac gtcaaggtgg
      601 gtctcacctt cgcatcgggc ctgcgatcca tcgtcaggca ggaccccgac gtgatcctgg
      661 tgggcgagat ccgagacctc gaaaccgccg agatagcgat ccaggctgcg ctcacgggcc
      721 acctcgtctt ctccacgctc cacacgaacg actcggcaag cgccgtgaca aggctgatcg
      781 acatgggcat cgagcccttc ctcgtcacct cgtcggtgaa cgccatcgtc gcccagcggc
      841 tcacgcgcaa gatctgctcc cactgcaggc agccctacca ccccgagagc gagaccctcc
      901 tcgaggtggg gctctccagg gaactgctcg ggagcgaggg gtacctctgg aggggcgccg
      961 gctgcaagga atgcctgggt accggttaca agggcaggac cggcatctac gagatcctcc
     1021 tcatgagcga ctcgatcaag tcgacgatcc tcaagacctc ggactccaac atcatcaaga
     1081 agcaggcggt ctcggagggg ctgcacagcc tgagggagga cggggcgcgg aaggtgaaag
     1141 acggcatcac caccatcgag gaggtcctca gggtcaccca gcagtgaccg gcccctggcc
     1201 gacgaaggtg cccgctcgag ccggggatgg tccaaaccgg ccgggacacc cgggcaagcc
     1261 ggcccccggc gtggatctcg cccctgcata cggcctcgat gggtgaggct tcgagcctcc
     1321 tcgccgatgg cgactggtca tcgatcttcc cggcgggtca gccctcgagg gcgcccatcc
     1381 cctcggcccg ggcggtgccg gcggcaaccc ggtgcatcga gcccctttgg gcggtaccgt
     1441 gatgtggggg cgcccccgaa cgggggatcc cgcttgcgcc tggagagca
//
LOCUS       DAOSCR010000660         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_57639_length_1488_cov_2.983950, whole genome shotgun sequence.
ACCESSION   DAOSCR010000660 DAOSCR010000000
VERSION     DAOSCR010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57639_length_1488_cov_2.983950"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="hflX"
                     /locus_tag="PLT69_12535"
     CDS             <1..876
                     /gene="hflX"
                     /locus_tag="PLT69_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ15312.1"
                     /translation="SDANLLVFDHELTPSQARSITEITDIRVIDSTQLILDIFARRAR
                     SREGKIQVELAQLRYNMSRLVGKNPFLSRLAGGIGTRGPGETKLEIDRRRAKERMDRL
                     EREAGRIALKRRLTRARRSRQRLPVISIIGYTNTGKSTLLNALTNSGVLTADMPFATL
                     DPSSKRLRFPRDTEAIITDTVGFIRDLPSDLRAAFRATLEELEDAQLLLEVIDLSDPN
                     IERRIEAVEEILRELGLAKKPRLKVFNKADRVGEQAAAQWASRYGGVAVSALDRSSLR
                     PLIERVEACFLDTPA"
     gene            complement(999..>1488)
                     /locus_tag="PLT69_12540"
     CDS             complement(999..>1488)
                     /locus_tag="PLT69_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOJ15313.1"
                     /translation="VNATRGEIRKYELVSKVFQLLNAYRIAMGRDVELLSRRFSYERI
                     HATFAIARGKMTFDDFSLESDSLQVFSAGTYDLDTRAIDAVVGVQPLESLDRTIGMIP
                     VVGWVFTGDSGRFIVVSMRVSGTMDDPKVALAPVDTLSNTVVATMLRSLKLPSKLVDD
                     SI"
BASE COUNT          262 a          526 c          472 g          228 t
ORIGIN      
        1 tccgacgcga acctcctcgt gttcgaccac gagctcacgc cctcgcaggc gaggtccatc
       61 acggagatca cggacatccg cgtcatagac agcacccagc tcatcctgga catcttcgcg
      121 cgcagggcga ggtcccgcga gggaaagatc caggtggagc tcgcccagct gcgctacaac
      181 atgtcgaggc tggtgggcaa gaaccccttc ttgagcaggc tcgcgggcgg catcgggacg
      241 cgggggcccg gcgagaccaa gctcgagatc gaccgcaggc gcgccaagga gcgcatggac
      301 aggctcgagc gcgaggccgg gaggatcgcc ctcaagcgca ggctcaccag agcgcgccgc
      361 tcacgccaga ggctgccggt gatctccatc atcggctaca cgaacaccgg caagtccacc
      421 ctcctcaacg cgctcaccaa cagcggcgtc ctgaccgcgg acatgccctt cgccacgctc
      481 gacccgtcca gcaagcgcct gcgcttcccg agggacaccg aggcgatcat caccgacacg
      541 gtgggcttca tccgcgacct cccctccgac ctcagggccg ccttccgggc gacgctggag
      601 gagctcgagg acgcccagct cctcctcgag gtcatcgacc tgtccgaccc caacatcgag
      661 cgcaggatag aggcggtcga ggagatcctc agggagctgg ggctggcgaa gaagcccagg
      721 ctcaaggtct tcaacaaggc cgaccgcgtg ggcgagcagg cggccgcgca gtgggcgtcc
      781 cgctacggcg gcgtcgcggt ctcggccctc gacaggtcgt cgctcaggcc cctcatcgag
      841 cgcgtggagg cctgcttcct ggacaccccc gcctgagggg aggcccctgc cccggcgccg
      901 tcttttaggg cggcctccca tgggcctctg caaggggagg tcctcgcggg gggtgtgcac
      961 ccgtggccct ctcacttagg cagcctcttc tggatggcct agatggagtc gtccacgagc
     1021 ttggagggca gcttgagcga gcgcaacatg gtcgcgacca cggtgttcga cagggtgtcc
     1081 accggcgcga gcgcgacctt ggggtcgtcc atggtcccgc tcacccgcat gctcaccacg
     1141 atgaacctgc ccgagtcacc ggtgaacacc caccccacca ccggtatcat gccgatggtc
     1201 ctgtccaggc tctccagggg ctgcacgccc acgacggcgt cgatcgccct cgtgtcgagg
     1261 tcgtaggtgc ctgccgagaa cacctggaga gagtcgctct cgagcgagaa gtcgtcgaaa
     1321 gtcatcttcc ccctggcaat ggcgaacgtg gcatgtatcc gctcgtagga gaaacgcctg
     1381 ctcaacagct ccacgtcgcg gcccatggct atcctgtacg cgttgagcag ctggaacacc
     1441 ttggacacga gctcgtactt gcggatctcg cccctggtcg cgttcacc
//
LOCUS       DAOSCR010000661         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_57688_length_1487_cov_2.742318, whole genome shotgun sequence.
ACCESSION   DAOSCR010000661 DAOSCR010000000
VERSION     DAOSCR010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57688_length_1487_cov_2.742318"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLT69_12545"
     CDS             <1..570
                     /locus_tag="PLT69_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOJ15314.1"
                     /translation="IRGYGPFLRERDPVERLRLFDTFRILCAVREGPYGVQALNRIVE
                     RVLASEGLIDPVDQFYENRPVLITENDYTVSLFNGEVGVVARGDEGVRVCFPGPRGEA
                     RMVSTTRLPAHETAFAMTVHKSQGSEFDHVLVILPDRDIPLLTRELVYTAVTRARQSV
                     TVWGVEKVFVQAVSRATRRVSGLRERLWA"
     gene            802..>1487
                     /locus_tag="PLT69_12550"
     CDS             802..>1487
                     /locus_tag="PLT69_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15315.1"
                     /translation="MARNITKAELAERVKELEREVSLLRRSFSERNRLDELFLETVGE
                     ACYLFDLKGNVMGFNREAQGMLGYEPGELPGLNYKRFTSPETARRLYECFHRIYTTGV
                     PQRMVDYEVIRKDGSRRIHEMSAGLVRDERGDPAGFRVLVRDVTEQRLMKALLEEGER
                     RYRTIFENTGTATIVIAADTTVLLANANFARLVGYSKEEIEGKMSWTVFVHPEDLERM
                     RRYHFMRREAP"
BASE COUNT          287 a          420 c          531 g          249 t
ORIGIN      
        1 atccgggggt acggtccctt cctgagggag agagaccccg tggagaggct gagactcttc
       61 gacacgttcc ggatcttgtg tgccgtgagg gagggaccgt acggcgtcca ggccctcaac
      121 cggatcgtcg agagggtgct cgcttcagag gggctcatcg accctgtgga tcagttctac
      181 gagaacaggc cggtcctcat taccgagaac gactacacgg tgagcctgtt caacggcgag
      241 gtgggggtgg tcgcgcgcgg tgacgagggc gtgcgggtgt gtttccccgg tccacggggc
      301 gaggcgagga tggtgtcgac cacgaggctg ccggcccacg agaccgcctt cgccatgacc
      361 gtgcacaaga gccagggttc cgagttcgac cacgtgctcg tgatcctgcc ggacagggac
      421 atccccctcc tgacgaggga gctcgtgtac acggccgtca cgagggccag gcagagcgtg
      481 acggtgtggg gcgtggagaa ggtgttcgtg caggcggtct cccgagccac caggcgcgtc
      541 tcggggttga gagaaaggct ctgggcgtaa ggatcggatc gcccgggaga cggtccaggc
      601 gggtcgccgt cgcctggccg ggttgccgtt tgctcatggg cgggtcttcg tgatcggcgc
      661 tcgagcgtcc ctgttggcgg tgcgtgaaca tggccgttcg gcagtcatac cgggagagaa
      721 ccttgccgat ataccttaag gggaattttc gaggtgggaa gaagcaccag gccggggaag
      781 gcacggttcg tggaggctga catggcgagg aatatcacca aggccgagct tgccgaaagg
      841 gtcaaggaac tcgagaggga ggtttccctc ctcagaagga gcttcagcga gagaaaccgt
      901 ctcgacgagc tcttcctgga gaccgtgggc gaggcatgct acctgttcga cctcaaggga
      961 aacgtcatgg ggttcaaccg cgaggcgcaa ggcatgctcg gctacgagcc cggcgagttg
     1021 cccgggctca actacaagcg cttcacctct cccgagaccg caaggcgcct gtacgagtgc
     1081 ttccaccgca tctacaccac cggtgtaccc cagcgcatgg tggactacga ggtcatccgc
     1141 aaggacggga gcagaaggat ccacgagatg tccgcggggc tcgtgcggga tgagcgcggg
     1201 gaccctgccg gtttccgcgt gctcgtgcgg gacgtgaccg agcagaggct gatgaaggcg
     1261 ctcctggaag agggagagag gcgttaccgt accatcttcg agaacaccgg cacagcgacc
     1321 atcgtcatag cggcagacac aaccgtcctg ctcgccaacg cgaatttcgc gaggctcgtg
     1381 ggctattcaa aggaggagat cgaggggaag atgagctgga cggtcttcgt ccaccccgag
     1441 gacctggagc ggatgaggcg ctaccacttc atgcggaggg aggcccc
//
LOCUS       DAOSCR010000662         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58013_length_1481_cov_3.107994, whole genome shotgun sequence.
ACCESSION   DAOSCR010000662 DAOSCR010000000
VERSION     DAOSCR010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58013_length_1481_cov_3.107994"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLT69_12555"
     CDS             <1..652
                     /locus_tag="PLT69_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOJ15316.1"
                     /translation="IHRAMLISKHMKSQRLAAVTKAAIAANHEINSPLTTILLKLDML
                     MGERQVPEPVKRSLAEIKHEVITIKNVVKRMLEISDVIDTTYARNEKMLDLRASQGAA
                     QGRQQHPGETASVHDEAGFESPPSTRSGPVLSPEAKSVSFDQIPGFEDYLEDKPSRSD
                     RGERSAGVLPHHDKPGFEDFLEDSTSGKKPGNQPSPKGQRDTDGTLTPGFEDLTDK"
     gene            949..>1481
                     /locus_tag="PLT69_12560"
     CDS             949..>1481
                     /locus_tag="PLT69_12560"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOJ15317.1"
                     /translation="MERLVYGNGRIQWGIYAEPVEEVNYLDYDLRTPMGLRIPRPLRR
                     LIANQFHFLGVIDPSVIVAAAVVDLKYLESAFLYVFDRRTHELVEVSDIAPGCARRAS
                     ISPTPESPSSRYASGRLSIGLSRDRFEAASRAIQVELSLEASHAAPLRICTRAGYTRW
                     VYTQKTSPLRVKGTLRLG"
BASE COUNT          300 a          483 c          466 g          232 t
ORIGIN      
        1 gatccaccgc gccatgctca tatcgaagca catgaaatcc cagcgtctcg ccgcggtgac
       61 caaggccgcc atcgccgcga accacgagat caactcgccg ctgaccacca tcctcctcaa
      121 gctcgacatg ctcatgggag agcgccaggt ccccgagccg gtgaagaggt ccctggcgga
      181 gatcaagcac gaggtcataa ccatcaagaa cgtcgtgaaa cggatgctcg agatctccga
      241 cgtgatcgac acgacgtatg caaggaacga gaagatgctc gacctcaggg catcccaggg
      301 tgcggcccag ggcaggcagc agcatccggg cgagaccgcc tccgtccatg acgaggccgg
      361 gttcgagagc cccccgagca ctcggtcagg tcccgtgctt tctccagagg cgaagtccgt
      421 gtcgttcgac cagatacccg ggttcgagga ctacctggaa gacaagccct cccggagcga
      481 tcgaggggaa cggtcggccg gggtcctgcc gcatcatgac aagccgggct tcgaggattt
      541 cctcgaggac tcaaccagcg gcaaaaagcc tggaaaccag ccatccccca agggacagag
      601 ggacaccgac ggcaccctca cccccggctt cgaggacctg accgacaagt gacagtcggc
      661 cgcggcgcgc cttgccgcgc actcggctgc agcccagaac gagtgcaggg cttcaggaag
      721 gatcagggca ggaagtgggc cttcccggag cttctcctca ccacccgcga aaggcctcga
      781 ccccccgtgt cgcatgaggc tggaccgccc ggccgggctc ttcaaaccgg ttgatcctcg
      841 ggtcatcatc gcgtattgat gggaaagggc gtcgctcacc ggcgtttcgg tacctgacac
      901 cgagggcgta ccgtgctcgc cgggatcacg tgaggggagg tccggaagat ggagaggctc
      961 gtctacggca acggccggat acagtggggg atctatgctg aaccggtcga ggaggtcaac
     1021 tacctcgact atgacctgcg cacacccatg ggcctccgta ttccacgacc cttgaggcgt
     1081 ctcatcgcga accagttcca cttcctcggc gtgatcgacc cgtcggtcat cgtggcggcg
     1141 gctgtggttg acttgaagta cctcgagagc gccttcctct acgtcttcga caggcgcacc
     1201 cacgaactcg ttgaggtgag cgacatcgcg cccggttgtg cacggagggc gtccatctct
     1261 cccacgcctg agagcccatc gagcaggtat gcatcgggca ggctgtccat agggctctcg
     1321 cgggacaggt tcgaggccgc gagcagggcc atccaggtgg agctctcact ggaagcgtcc
     1381 cacgcggctc ccctgcgcat atgcaccaga gcgggctaca cgaggtgggt ctacacccag
     1441 aagacgagcc cgctgagggt caaggggacg ctgaggctcg g
//
LOCUS       DAOSCR010000663         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58148_length_1479_cov_3.744382, whole genome shotgun sequence.
ACCESSION   DAOSCR010000663 DAOSCR010000000
VERSION     DAOSCR010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58148_length_1479_cov_3.744382"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLT69_12565"
     CDS             <1..979
                     /locus_tag="PLT69_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15318.1"
                     /translation="VVLDLAPSRFFSVHLVFGAAVVSLALISYLTAFRVLGGPGYASA
                     SLFAGEHTGLFGSLLSVFSSGIPWVFMLIPAFIQGDGPSDQDRWRRLLPLRIACIAAP
                     LALWLTGRSMPGMSALLVAPASVLAGAWAASSLEGTTRRRPIDSWMLLCSGISVLAVA
                     SAAMIRTALSDDGVSIALAAWAAVFTAMALAFAVLSIKRRTVGRLVLVAACSACLGWM
                     TASSEPWGAWERKAAFARDASVHSPLVVHEDDLVMRGLLGLAGTGCMVVPRSAVPLGA
                     KAYLAASSHDLDGLVTSLSERMEATVVDALHERTSYAVILIGPPPEVRP"
BASE COUNT          196 a          539 c          477 g          267 t
ORIGIN      
        1 cgtcgtgctc gatctcgcgc cctcgcgatt cttctccgtg cacctggtct tcggcgcagc
       61 cgtcgtctcg ctcgcgctca tctcctacct cacggccttc agagtcctcg ggggcccggg
      121 ctacgcaagc gcatcgctct tcgccggcga gcacacgggt cttttcgggt cactgctctc
      181 ggtcttctct tcgggcatcc cctgggtgtt catgctgata cctgcgttca tccagggaga
      241 cggcccgtcg gaccaggatc gctggaggcg cctgctgccc ctgaggatcg cctgcatcgc
      301 cgctcccctt gccctgtggc tcaccggccg ttcgatgccc gggatgtccg ccctccttgt
      361 cgcgcccgcg tccgtactcg ccggggcgtg ggcggcctcc tccctcgagg gcaccacgag
      421 acggcgcccc attgactcgt ggatgctctt gtgctcgggt atcagcgttc ttgccgtcgc
      481 gtctgcggcc atgatccgta ccgcgctctc agacgacggc gtgagtatcg cccttgcggc
      541 ctgggcggct gtcttcaccg ccatggcgtt ggctttcgcg gtgctgtcga tcaagcgccg
      601 caccgtggga aggctcgtcc tcgtagccgc gtgttccgcg tgcctgggct ggatgaccgc
      661 gtcctcggag ccgtggggcg catgggagag gaaggccgcc ttcgcgcgcg atgcatccgt
      721 gcactccccc ctggtcgtcc acgaggacga cctcgtcatg cggggcctcc tgggtcttgc
      781 gggcacgggg tgcatggtgg tgccgaggtc ggccgtcccg ctcggcgcga aggcatacct
      841 ggctgcgagc tcgcacgacc tcgacggtct tgtcacgtcg ctgagcgaac gcatggaagc
      901 gaccgtcgtc gatgcgctcc acgagaggac cagctacgcc gtcatcctca tcggcccgcc
      961 cccagaggtc aggccctgac gaaggcccac gcctcgacgg tgcaagccgt caatgaccgg
     1021 agaagttcgc tctttcaaga acgccgaagc gagcggcatc ctcgtcacaa cggcgaaggc
     1081 cgaaccccac gtggccggcg cccgggacgt tccttgagat ccggccttga cccaacgcac
     1141 accgccaggc cgattccccc accatgacca tgcccgctga cagcgggcaa gacccgagcc
     1201 ttggcgagag cgactctccc gggcacggcc gcgaccttga cccgagacac tcgatcccgg
     1261 gatgggtgcg ttcaccaggg agaaccgatc ggggccacgc cttctcgagg cgcctgcacg
     1321 aggtcgaccc cttcgtcggc acaccccctc atcacgtccg actctcgtgg ggtcgaaccg
     1381 tttcgccccg gggcggacct ctcaaggagg gcaggggcga tgcggccccg aggccttctc
     1441 ttgtctcgtc cccgggggcc cgccgggggg ccggggagg
//
LOCUS       DAOSCR010000664         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58161_length_1479_cov_2.665730, whole genome shotgun sequence.
ACCESSION   DAOSCR010000664 DAOSCR010000000
VERSION     DAOSCR010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58161_length_1479_cov_2.665730"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLT69_12570"
     CDS             complement(<1..362)
                     /locus_tag="PLT69_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15319.1"
                     /translation="MMKILCALGTAVFLLLAARPGFGATDVIVGGTETKTRVVIYLGD
                     NDTISPELEAKDLTLSVKFPYRVAQPRTISDRFLIDTLVFDGTRATITMKKPFAHTWQ
                     LKQVPSRLVIDIADRSGGR"
     gene            complement(934..>1479)
                     /locus_tag="PLT69_12575"
     CDS             complement(934..>1479)
                     /locus_tag="PLT69_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ15320.1"
                     /translation="NLEEEVAQGRFSEDLYYRLNVIPIHIPPLRERTEDIPLLVKHFL
                     AKYADGKAKKAPEFSKKAMSTLMRYRWPGNVRELENLVERMVVLCDSDTIDVADLPDK
                     ISAGPAEASPLAPFISLPEEGIDLSTAVNEFERSIIIQALNRSNWVKNRAAKLLNLNR
                     TTLVEKIKKQKLERPTEPSMT"
BASE COUNT          228 a          456 c          499 g          296 t
ORIGIN      
        1 cgcccccccg atcggtcggc gatgtctatg acgaggcggg acggaacctg cttgagttgc
       61 caggtgtgtg cgaagggttt cttcatggtg atcgtcgccc tcgtcccgtc gaagacgagc
      121 gtgtcgatca ggaaacggtc cgagatggtc ctcggctggg ccaccctgta gggaaatttg
      181 acggagagcg ttagatcctt cgcctcgagc tcggggctta tggtatcgtt gtccccgagg
      241 tagatgacca cgcgggtctt ggtctccgtc ccccccacga tcacgtccgt ggcgccgaag
      301 cccggccgtg ccgcgaggag gagaaagacc gcggtcccca gggcgcagag tatcttcatc
      361 acatgggttc gagttgtgtt ctcgatcttc accgaggcac ggtgatacaa ctttcatgcc
      421 atcgcggcgg cgccggccgc ggggtgggta ggcccggcgg aggaccctgt ccgaggggtg
      481 ctgcgggcag gacacccgga aagggggaag accgtgtgcc cgtgggctgc cgtaaggttt
      541 cttggaccgc gcacggccgg tctccaaaga aagaccgtgt gcccgtgggc ccccggtcac
      601 gtaagcgacg cgcctcgaac ccgggcgcgt gcggcctgta cttgtccaga tacgtccgta
      661 ccatcccggg ttacctcgaa cccgcgcgcg tgcggcctgc acccgatccc acgcgccgag
      721 aagaggggag agcgtgaggc aggccgccgt gcagcccgtc tcgtatgcac acaacaccgt
      781 gcatcccttc tgcctcggga tgcgatacgc ggtgtacatg cggcgcctcg gtgctcccgt
      841 gagacggaca tgctgcgggg accctgacga tgtgagaccc ttccttggaa ggagggcttc
      901 gatgcgcccg tgagacggcc gtgcccggcc gggtcaggtc atgctcggct cggtcggtct
      961 ctcgagcttc tgcttcttga tcttctccac gagggtggtg cggttcaggt tgaggagctt
     1021 ggccgcgcgg ttcttcaccc agttgctccg gttcaaggcc tgtatgatga tgctccgctc
     1081 gaactcgttc accgcggtgg acaggtcgat gccctcctcg ggcaggctga tgaagggcgc
     1141 aagcggcgag gcctcggcgg ggcccgccga gatcttgtcc gggaggtcgg ccacgtctat
     1201 ggtgtcgctg tcgcacagga ccaccatgcg ttcgacgagg ttctccagtt cgcggacgtt
     1261 gccgggccag cggtacctca tgagtgttga catggccttc ttgctgaact ccggggcctt
     1321 cttcgccttc ccgtccgcgt acttcgccag gaagtgcttc acgagcagcg gtatgtcctc
     1381 ggtacgctcc ctcaaggggg ggatatggat cggtatgacg ttcaggcggt agtagaggtc
     1441 ttcactgaac cgaccctggg cgacctcctc ttccaggtt
//
LOCUS       DAOSCR010000665         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58515_length_1473_cov_2.544429, whole genome shotgun sequence.
ACCESSION   DAOSCR010000665 DAOSCR010000000
VERSION     DAOSCR010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58515_length_1473_cov_2.544429"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PLT69_12580"
     CDS             <1..986
                     /locus_tag="PLT69_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ15321.1"
                     /translation="GVGDDALAGLVDRLGVDRLVSGEVDISKFKDESRSRRYDVRMRL
                     RPEDRANPSDLGNIAVRAADGRLVELSNLVTIVEGGGPTSISRYNRQRSTWVFANLEN
                     KPLAEAMAELDSIASRILPAGYRTAYVGQAETMGESFKYLLFALLLGIVLAYMVLAAQ
                     FESFLHPITVLLAMPLSFIGAFGALLVTGMTISIVSFIGLILLMGLVKKNSILLVDYT
                     NTLRERGYSRRDAILQAGPVRLRPILMTTFAMVFGMLPVALGIGEGSDIRAPMGVIVI
                     GGLLTSLFLTLAVVPAAYDLFDDWKQGIGRWVRSRMRTGHAQESPEGGDAR"
     gene            983..1273
                     /locus_tag="PLT69_12585"
     CDS             983..1273
                     /locus_tag="PLT69_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15322.1"
                     /translation="MKMVFVVYSRAADYDVIKALKDAGITAYTKMERACGEGLETEPK
                     LHTHTWPGENCVLFAAVGIEEYERLTGVIRELKARHPRAGLKAFVLPMEEII"
BASE COUNT          277 a          444 c          486 g          266 t
ORIGIN      
        1 ttggggttgg cgatgatgcc cttgcgggct tggttgatcg cctcggggtt gaccgcctcg
       61 tgagcggcga ggtcgacatc tcgaagttca aggacgagtc gcgctcgagg cgctacgacg
      121 tgaggatgcg cctcaggccc gaggacaggg caaacccctc ggatctcggc aacatagcgg
      181 tgagggccgc cgacgggagg ctcgtggaac tctccaacct ggtcaccata gtcgagggcg
      241 gggggccgac cagcatcagc aggtacaacc gccagaggtc cacgtgggtc ttcgccaacc
      301 tcgagaacaa gcccctggcc gaggccatgg ccgagctcga ctcgatcgcc tcgaggatcc
      361 tgcccgcagg gtacaggacg gcgtacgtgg gccaggccga gaccatgggt gaatctttca
      421 agtacctcct gttcgcgctc ctcttgggca tcgtcctcgc ctacatggtg ctcgcggccc
      481 agttcgagag cttcctccat cccataacgg tcctcctggc catgccgctg tcgttcatcg
      541 gagcgttcgg cgccctgctc gtgacgggca tgaccataag catcgtgagc ttcatcgggc
      601 tcattctcct catggggctc gtgaagaaga actccatcct gctcgtggac tacacgaaca
      661 cgctgagaga gcgcggctac tcgaggaggg acgccatcct ccaggcaggg cccgtgaggc
      721 tcaggccgat cctcatgacc accttcgcca tggtgttcgg catgcttccc gttgcgctgg
      781 gtatcggcga gggctccgac atcagggccc ccatgggggt gatcgtgata ggcggtctcc
      841 tgacctcctt gttcctcacg ctcgcggtgg tgccggctgc gtacgacctc ttcgacgact
      901 ggaaacaggg gatcgggaga tgggtgaggt cgcgtatgcg tacagggcat gctcaagagt
      961 ctcccgaagg gggcgatgcg agatgaagat ggtcttcgtg gtctattcga gggcggccga
     1021 ttacgacgtg atcaaggcgc tcaaggatgc gggcataacg gcctacacca agatggaacg
     1081 cgcctgcggg gaagggctgg agaccgagcc gaagctccac acccacacct ggccggggga
     1141 aaactgtgtc ctcttcgccg cggtgggcat cgaggagtac gagcgcctca ccggggtaat
     1201 ccgggaactc aaggccaggc atccacgggc tggactcaag gccttcgtcc tgcccatgga
     1261 ggagatcatc tagcgacgaa ggtgcctcga tgcctcgtgg cgactttcgg tccagccggg
     1321 ccggcatgga tgggatcgtc ttgcgcaagg cgccgcgcat agcgtatgat cactgtctgc
     1381 agagagcgcc gacgtggtca tgatgaaaga catcctctac gagaagaccg agcagaggct
     1441 gctcgccaag gagctcgacc cggcgaaatc gac
//
LOCUS       DAOSCR010000666         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58554_length_1472_cov_3.500353, whole genome shotgun sequence.
ACCESSION   DAOSCR010000666 DAOSCR010000000
VERSION     DAOSCR010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58554_length_1472_cov_3.500353"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLT69_12590"
     CDS             complement(<1..769)
                     /locus_tag="PLT69_12590"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ15323.1"
                     /translation="MKSTSFDVHALPVLPEIADAVIRMALDEEVTVAKIADLIEKDQA
                     LTVRILELANSSFYRRSRQIHTVKEAVVLIGAEAVRTLVLGISVMEIFPDRQGSRLDH
                     RAFWRHCLGSALYAEEIMGAKNHALGAKAFCAGLLHDVGKLVLESVRPDEYGRVLEQA
                     REGLRPLDAIEREILGLSHADAGRDVLAHWKLPGIYEETVWCHHEPVLILDDDQYRVS
                     GAVHVANALAHMSGMGSSGNFFPQAVAEALLKSLALGD"
     gene            complement(766..>1472)
                     /locus_tag="PLT69_12595"
     CDS             complement(766..>1472)
                     /locus_tag="PLT69_12595"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ15324.1"
                     /translation="LGLLVDSGFEELEIGCQSLDDPVLEASGRGHTVKDVVEACRLVR
                     GFGLRLGIQLMPGLPSEDLASFVTTVKRSAALKPHAARIYPTVVMAGTRLEGLYRTGA
                     YTPLTLGQAVRMTLYAVAVLERQGCTILRMGLPDPGAQNVVAGPYHPSLGFLVRSEAY
                     RVMAGLALSRHGGGARLVVNPRSLCELLGPGRANARELTFAYGCDDTLPVGTLRVEAG
                     TDRACIQLQDILEYIL"
BASE COUNT          255 a          511 c          466 g          240 t
ORIGIN      
        1 catcgccgag cgccaggctc ttgaggaggg cctcggcgac cgcctgcggg aagaagttgc
       61 ccgacgaacc catgccgctc atgtgggcaa gcgcgttcgc cacgtgcacc gcgcccgaga
      121 cccggtactg gtcgtcgtcc aggatcagca cgggctcgtg gtggcaccac acggtctcct
      181 cgtagatgcc ggggagcttc cagtgggcca gcacatcccg cccggcgtcg gcgtgggaaa
      241 ggccgaggat ctccctctcg atggcgtcca ggggcctcag gccttcccgt gcctgctcga
      301 gcacacggcc gtattcatcg ggtctcaccg actccagcac gagcttgccc acgtcgtgga
      361 gaagacccgc gcagaacgcc ttggcgccga gggcatggtt ctttgcgccc atgatctcct
      421 cggcgtagag ggccgagccg aggcagtgtc gccagaacgc gcgatgatcg agcctggacc
      481 cctgcctgtc ggggaagatc tccatgacgc tgatccccag caccaaggtc ctcacggcct
      541 cggccccgat gaggacgacc gcctccttga ccgtgtggat ctgccgcgac ctcctgtaaa
      601 agctcgagtt cgcgagctcc aggatgcgta cggtaagggc ctggtccttc tcgatgagat
      661 cggcgatctt ggctaccgtg acctcctcgt ccaaggccat cctgatcacc gcatccgcga
      721 tctccggaag caccgggagc gcatgcacat cgaaagatgt cgatttcata aaatatattc
      781 tagaatgtcc tgcagttgta tacaagcacg atctgtaccg gcctccaccc tcagcgtccc
      841 cacgggtaac gtatcgtcac aaccgtatgc gaacgtgagc tcgcgcgcat tggcgcggcc
      901 cggccccaag agctcgcaga ggctcctcgg gttcacgacg agccgggcgc cgccgccgtg
      961 gcgcgacagg gcgaggccgg ccatgaccct gtacgcctcc gatcgcacga ggaacccgag
     1021 ggaggggtgg tagggcccgg ccaccacgtt ctgcgctccg gggtcgggca gccccatgcg
     1081 caggatggtg cagccttgcc gctcgagcac ggccaccgcg tagagggtca tcctgacggc
     1141 ctgccccaag gtgaggggcg tgtacgcacc cgtgcggtag agaccctcga gccttgtccc
     1201 ggccatgacc acggtggggt agatcctcgc cgcatgcggc ttcagggccg cggagcgctt
     1261 caccgtggtc acgaaagacg cgaggtcctc cgagggaagc ccgggcatga gctggatgcc
     1321 cagcctcagc ccgaaccccc tcacgagcct gcaggcctcg acgacgtcct tgaccgtatg
     1381 ccccctgccg ctcgcctcga gcacagggtc gtccagggac tggcagccga tctcgagctc
     1441 ctcgaagccg ctgtccacca ggagcccgag tt
//
LOCUS       DAOSCR010000667         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58559_length_1472_cov_2.959068, whole genome shotgun sequence.
ACCESSION   DAOSCR010000667 DAOSCR010000000
VERSION     DAOSCR010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58559_length_1472_cov_2.959068"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLT69_12600"
     CDS             complement(<1..632)
                     /locus_tag="PLT69_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ15325.1"
                     /translation="MTWTDTGLQPELFAHVDEHFAHFMERLSGSPSKELALAAALLSR
                     VCREGHTCLDLADPLSALDPGSRASLEKLIPGVEAWVDSLRGLPVVGGPGDYRPLVLD
                     GENRLYLHRIHEDELFLSNRLRELSSQVRPLDVDRLREVLTKTLKGACDEGTVRQKAA
                     VIAALRSSLCVVTGGPGTGKTTLVLRMLALMMENDPGLVVRLAAPTGKAAR"
     gene            complement(629..>1472)
                     /locus_tag="PLT69_12605"
     CDS             complement(629..>1472)
                     /locus_tag="PLT69_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HOJ15326.1"
                     /translation="LTRGVSSEHVDQARDIDHDPLATQPDMAERKDAFSFPAGAASGV
                     LLHEIFEKIDFAAGAGEIQEMCRQALVSHGFDPGWTDTVSSMVQRVLGADLGGFSLRD
                     VGATNRLSELEFFYPLRLVGWRELGDALEACIGCDLPAGQGLKTPRLVFDPIRGFVRG
                     FMDLVFSHGGKFYLVDWKSNRLGENPSVYSPASLQEAMLRNNYVLQYLLYLVGLDLYL
                     ASRVPGYRYETHFGGVFYVFLRGVNPGWGAGSGIYYARPSTAALGRIRAALVGGPGTG
                     GPLP"
BASE COUNT          244 a          513 c          490 g          225 t
ORIGIN      
        1 cgggccgcct tgccggtcgg cgccgccagg cgtaccacga gcccgggatc gttctccatc
       61 atgagggcga gcatcctcaa cacgagcgtc gtcttgcccg tgcccggccc gccggtgacc
      121 acgcacagcg agctcctgag cgcagcgatg acagcggcct tctgccggac ggtcccctcg
      181 tcgcacgcgc ctttgagcgt cttggtcaac acctccctca gccggtccac atcgagcggc
      241 ctcacctgag agctcagctc tctcagcctg ttcgacagga agagctcgtc ctcgtggatg
      301 cggtggaggt agagcctgtt ctccccgtcg aggacgaggg gcctgtagtc accgggcccg
      361 cccaccaccg gcagcccccg caggctgtcc acccaggcct cgaccccagg tatgagcttc
      421 tccagcgacg ccctggaacc cgggtcgagg gccgacagcg ggtccgccag gtcgaggcag
      481 gtgtgtcctt ccctgcacac cctgctgagc aatgcagcgg ccagggccag ctccttggac
      541 ggcgagcccg acagtcgctc catgaagtgg gcgaagtgtt cgtcgacgtg ggcgaagagt
      601 tccggctgca ggccggtatc ggtccatgtc acggcaaagg cccccctgtc ccgggaccac
      661 cgacgagcgc cgcccgtata cgcccgagcg ccgccgtgga gggtctcgcg tagtagatac
      721 ccgaccccgc gccccatccg gggttcacac ccctgaggaa cacgtagaac acgccgccga
      781 agtgggtctc gtacctgtac ccgggcacgc gggaggcgag gtagaggtcg agaccgacga
      841 gatagaggag gtactggagc acgtagttgt tcctgagcat ggcctcctgc agcgaggcgg
      901 ggctgtagac cgaggggttc tcgccgaggc ggttcgactt ccagtccacg aggtagaact
      961 tcccgccgtg cgagaacacg aggtccatga aacccctcac gaagccgcgg atagggtcga
     1021 agacgagccg gggggtcttc agcccctggc cggcggggag gtcgcagccg atgcacgcct
     1081 cgagggcgtc gccgagttcg cgccagccta cgagcctgag cgggtagaag aactcgagct
     1141 cgctcaggcg gttggtggca ccgacgtccc tcaggctgaa gccgcccagg tccgcaccga
     1201 gcaccctctg caccatgctc gagaccgtgt ccgtccaccc cgggtcgaac ccgtgggaga
     1261 caagcgcctg ccggcacatc tcctggatct cgccggcgcc ggcggcgaag tcgatcttct
     1321 cgaagatctc gtggaggagc accccggagg cagccccggc agggaaggag aaggcgtcct
     1381 tcctctctgc catgtccggc tgcgtcgcga gcgggtcgtg gtcgatgtcc cgcgcctggt
     1441 ccacgtgctc gctcgagacg cccctggtca gg
//
LOCUS       DAOSCR010000668         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58850_length_1467_cov_3.293909, whole genome shotgun sequence.
ACCESSION   DAOSCR010000668 DAOSCR010000000
VERSION     DAOSCR010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58850_length_1467_cov_3.293909"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..438
                     /locus_tag="PLT69_12610"
     CDS             130..438
                     /locus_tag="PLT69_12610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ15327.1"
                     /translation="MKNRRRQRRVRIPEKVGATVTYCDALKNAPKGLVGDISGGGIYM
                     FTDETLEKDSYVTMRIHTERYLGKPVYVQGLVVRTDESGMAIQFTYANDEDISSLLNF
                     "
     gene            complement(460..792)
                     /locus_tag="PLT69_12615"
     CDS             complement(460..792)
                     /locus_tag="PLT69_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOJ15328.1"
                     /translation="MTEAEKLYATLKPVQEARGYEFNADTTLVMELWEGLLFNKRRYG
                     YMSCPCRLASGDRGRDRDIECPCAYREQDVREYGSCYCGLYVSRQWNEGKIPRLYVPE
                     RRPVQETG"
     gene            complement(789..1043)
                     /locus_tag="PLT69_12620"
     CDS             complement(789..1043)
                     /locus_tag="PLT69_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOJ15329.1"
                     /translation="MKKSVMMFTLSTCSHCKAAKRFLNEHNVEFSFKDVDLLEAGERE
                     EALDEVRGYNPKLTFPTIVIDGRQVIVGFQDREIKEALGL"
BASE COUNT          288 a          477 c          421 g          281 t
ORIGIN      
        1 ctgcgacgat tctcggttcg gccgtcccta agtcttctcg agacgcgctt cggtgtgatg
       61 aaatccggca ttcggggcag aagcgagatc cccgctcgtc gcggaaaaac agtctgggag
      121 gacgttctca tgaagaaccg ccgaaggcag cgaagggttc gtatcccaga gaaggtgggg
      181 gccaccgtca cctactgcga cgccctcaag aacgccccga agggtctcgt gggcgatatc
      241 agcggcgggg gcatctacat gttcaccgac gagaccctgg aaaaggactc ctacgtcacc
      301 atgaggatcc acacggagcg ttacctcggg aaacccgtct atgtgcaagg gctcgtggtg
      361 cgcaccgacg agagcgggat ggcgatccag ttcacgtacg ccaacgacga ggacatctcc
      421 tccctcctca acttctgagg aggaacccgg ccgcttgtct caccccgtct cctggacggg
      481 ccttcgctcc gggacgtaga gacgggggat cttgccctcg ttccactgcc tcgagacgta
      541 aagaccgcaa tagcagctcc cgtattccct cacatcctgc tcccggtagg cgcacggaca
      601 ctcgatgtcc cggtccctcc ctctgtcgcc ggaggcgagc ctgcacgggc agctcatgta
      661 tccatagcgc ctcttgttga agaggagccc ttcccaaagc tccatgacga gggtggtatc
      721 cgcgttgaac tcgtaacccc tggcctcctg cacgggcttg agcgtcgcgt agagcttctc
      781 ggcctcggtc atagcccgag cgcctccttg atctccctgt cctggaaccc gacgatcacc
      841 tgccgaccgt cgatgacgat ggtgggaaag gtcagcttgg ggttgtatcc cctcacctcg
      901 tcgagcgctt cttccctctc cccggcctcg agcaggtcca catccttgaa ggaaaactcc
      961 acgttgtgct cgttcaagaa cctcttggcc gccttgcaat gactgcaggt actcagggtg
     1021 aacatcatga ccgatttctt catggatcct ccttgtcttg catacaccat aaggccgatg
     1081 atcgggcgtt tcaaggccgg tgcggtcccc gttcactcgg tagagcgcgg caccccgtcg
     1141 ctccgggcgc aggcggtgtg cacgcaaccg gccaggcaca cacggctcgc tggtacgtgc
     1201 agccagggcc ccgaatgtgc atgacgccac gagaacgtgc atgacgccac gagaacgcac
     1261 ccgcaccgcc gctcggctca tgccgtcagc acccaagagg cccaccctcg gtgagccctc
     1321 agcgtgttga cagaacgacg cttttcggac atcatggtgg cgacagagca gtggaggtca
     1381 cccatggaac ccatagagca gaacgtcggg accatagaca gggtcaatcg cgtgttgatt
     1441 gcactgggca ttctcatatt cctgacg
//
LOCUS       DAOSCR010000669         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_58912_length_1466_cov_3.512403, whole genome shotgun sequence.
ACCESSION   DAOSCR010000669 DAOSCR010000000
VERSION     DAOSCR010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58912_length_1466_cov_3.512403"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PLT69_12625"
     CDS             complement(<1..1165)
                     /locus_tag="PLT69_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ15330.1"
                     /translation="MNKRLVFVSALVLIVLSPFVPRLAVAATKPYVFGLLLVGPYNDK
                     GYSQAHYEGGRYVEKTVPGTKMIYIDKVNPSDRPGVTIVQLVDDMVEKGARLVIANSD
                     DMKDGIREAARKHPNIHFVHVSGDDVLTGKAPSNLVNIFGRMEYGKMMAGFAAALTTK
                     TGKIGYLGPLINEETRRLASAAYLGARYAWEKVIKRNPNDLTFQVTWIGFWFNIPGVT
                     ADPSQVAKNFYDSGFDVVISGIDTTEAVMVAKQKVNEGKKVWAIPYDYVGACEGGSSV
                     CLGVPYFNWGPGYASLVKKSMSGHWKPEWIWLGPDWKDINNPATSTVGFMVGDALSQE
                     ARKALKDFIAGLASGKVQLFKGPLNFQDGRVFLKKGEVATDRQIWYLPMLLQGM"
BASE COUNT          251 a          459 c          453 g          303 t
ORIGIN      
        1 tcatgccctg gaggagcatg ggcagatacc agatctgcct gtcggtggcc acctcgccct
       61 tcttgaggaa gaccctgccg tcctggaagt tgagcggccc cttgaacagc tgcaccttgc
      121 cggaagccag gcctgcgata aagtctttga gcgcctttct cgcctcctgc gacagggcgt
      181 cgccgaccat gaagcccacc gtcgaggtcg ctgggttgtt gatgtccttc cagtcgggac
      241 cgagccatat ccactccggc ttccagtggc cggacatcga tttcttcacg aggctcgcat
      301 acccgggtcc ccaattgaag tacggcacgc cgaggcatac cgacgacccg ccctcacatg
      361 cgccgacgta atcgtagggg atcgcccaga ccttcttgcc ctcgttcacc ttctgcttgg
      421 cgaccatcac cgcctcggtg gtgtcgatgc cggagatcac gacgtcgaag ccggagtcgt
      481 agaagttctt ggccacctgc gagggatccg cggtgacgcc tggtatgttg aaccagaacc
      541 cgatccaggt cacctggaag gtgaggtcgt tcgggttgcg cttgatcacc ttctcccagg
      601 cgtaccgggc tcccaggtac gcggcggagg cgagccgcct cgtctcctcg ttgatgaggg
      661 ggccgaggta gccgatcttg ccggtcttcg tggtgagggc cgcggcgaac ccggccatca
      721 tcttcccgta ctccatcctg ccgaagatgt tgaccaggtt cgacggggcc ttgcccgtca
      781 gcacgtcgtc gccggagacg tgcacgaagt ggatgttcgg gtgcttcctc gccgcctccc
      841 tgatcccgtc cttcatgtcg tcggaattgg ctatgacgag cctggcgccc ttctccacca
      901 tgtcgtctac gagctgcacg atggtcacgc cgggcctgtc ggacgggttc accttgtcga
      961 tgtagatcat cttcgttccc gggacggtct tttccacgta acgcccgccc tcgtagtgag
     1021 cctgtgagta ccctttgtcg ttgtacggtc ccaccagcag caggccgaac acgtaaggtt
     1081 tcgtcgctgc gactgcaagc cgtgggacaa agggcgacaa aacgatcagc accagtgcgg
     1141 aaacgaagac cagcctcttg ttcatggtga ccatccccct ctgcaaggtt tgtggatgaa
     1201 cctgtgtcgt cgacctgcaa gtatggtgca gagtatatgg ggaaacgccc cggagggcaa
     1261 ggtgcaagga tgccctgggt gacgcggggg gtacgagtct tggagtatgg gggttgcccc
     1321 ccatggaaag cgggccccac gacggtcgac ggggcccgca gacgtcctgt tcgggcaaag
     1381 tgaccgacta cccggccacc cgcttgatcg tcctgtccgc gatgaggtcg ttgagctcct
     1441 ggaggaggac cttctcgtgg gcgtcg
//
LOCUS       DAOSCR010000670         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_59092_length_1463_cov_3.171165, whole genome shotgun sequence.
ACCESSION   DAOSCR010000670 DAOSCR010000000
VERSION     DAOSCR010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59092_length_1463_cov_3.171165"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="tssK"
                     /locus_tag="PLT69_12630"
     CDS             complement(<1..885)
                     /gene="tssK"
                     /locus_tag="PLT69_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HOJ15331.1"
                     /translation="MERPLLWHQGLFLQPQHFQLADLYHSSLLWPVYRYLSPHAWGVA
                     RMDIRAASLGTGRFHIDSGEFIFPDATYAVVPGNAVVEPRSFEDAWKDGGRPLNVYLG
                     LRRFNRMGPNVTERRKEPPSAGLNTRFVADEAPVECVDLHLDGPPAQVTRMTYVLKIL
                     WETEKDFFGEYETIPVARLERRGEEVVVSEDFIPPSVTCEASQALVRMIRDIRDLIAS
                     RGFQLEGYKRDRGVHTAEFGSRDMVYLLALRSLNRFIPRLSHLLETPTHPWVVYGVLR
                     ELVGELSTFSERCSVVGEL"
     gene            complement(953..>1463)
                     /gene="tssJ"
                     /locus_tag="PLT69_12635"
     CDS             complement(953..>1463)
                     /gene="tssJ"
                     /locus_tag="PLT69_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HOJ15332.1"
                     /translation="IAAALCAWSCASGPAQVRWDYEKDALELHLTADRNLNFKDKKAH
                     ALVLCVYQLSSPNAFEQLAGSRDGLYRLLECQAFDPQTVSVCKQVVAYPGKDVTVKLD
                     RAEGARYVGIVAGYYALERGKITRIYRIPEVTERRGFLWLSKVRKPGRLSVNLVLGAR
                     NLEDPPPSK"
BASE COUNT          239 a          488 c          473 g          263 t
ORIGIN      
        1 cagctcgcct acgacggagc accgttccga gaacgtggag agctccccga cgagttctct
       61 caagaccccg tagacgaccc acgggtgggt gggggtctcg aggaggtggg agagcctcgg
      121 gatgaagcgg ttgagcgagc gcagggccag gaggtagacc atgtccctgg aaccgaactc
      181 cgcggtgtgg acgccgcggt cgcgcttgta gccctccaac tggaaccccc tcgacgcgat
      241 gaggtcgcgg atgtccctga tcatccgcac gagcgcctgc gatgcctcgc acgtgacgct
      301 cggggggatg aagtcctcgg acaccacgac ctcttccccc ctcctctcga gcctggccac
      361 gggaatggtc tcgtactcgc cgaagaagtc cttctccgtt tcccacagga tcttcagcac
      421 gtaggtcatc ctcgtgacct gcgcgggggg cccgtcgagg tggagatcga cgcactcgac
      481 gggcgcctcg tcggcgacga accgcgtgtt caacccggcc gacggcggct ctttccgcct
      541 ctcggtcacg ttcgggccca tgcggttgaa cctgcgcagg ccgaggtaga cgttcaaggg
      601 ccttcccccg tccttccagg cgtcctcgaa cgagcggggc tcgaccaccg cgttgcccgg
      661 gaccacggcg tacgtcgcgt cggggaagat gaactccccg gagtctatgt ggaacctgcc
      721 cgtgcccagc gaggccgccc tgatgtccat cctggcgaca ccccacgcgt gcggggagag
      781 gtagcggtac acgggccaga ggagagagga gtggtaaagg tcggcgagct ggaagtgctg
      841 cggctggagg aacagcccct ggtgccacaa gagaggtctt tccatcggat gtccttctcc
      901 ttttgtgtaa ggtgggggtc tttctcccct tgcggggcac gaaacgccgg gttcacttcg
      961 aaggcggcgg atcctcgagg ttcctggcgc cgagcacgag gttcacgctc agcctgccgg
     1021 gcttcctgac cttcgagagc cacaggaacc ccctgcgttc ggtcacctcg ggtatccggt
     1081 atattctcgt gatcttgccg cgctccagcg catagtaccc ggccacgatc cccacgtacc
     1141 tggccccctc ggccctgtcc aacttcacgg tcacgtcctt gccgggatag gccaccacct
     1201 gcttgcacac cgagaccgtc tgcgggtcga acgcctggca ctcgaggagc cggtagagcc
     1261 cgtccctgct cccggcgagc tgttcgaagg cgttggggct gctcaactgg tacacgcaga
     1321 ggaccagggc gtgcgccttc ttgtccttga agttgaggtt cctgtcggcc gtgagatgca
     1381 gctcgagggc gtccttctcg tagtcccacc tcacttgggc cgggcccgag gcgcacgacc
     1441 acgcacaaag ggccgccgcg att
//
LOCUS       DAOSCR010000671         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_59216_length_1461_cov_3.687767, whole genome shotgun sequence.
ACCESSION   DAOSCR010000671 DAOSCR010000000
VERSION     DAOSCR010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59216_length_1461_cov_3.687767"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..>1461
                     /gene="serA"
                     /locus_tag="PLT69_12640"
     CDS             366..>1461
                     /gene="serA"
                     /locus_tag="PLT69_12640"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ15333.1"
                     /translation="MKILVSDKLSEAGVDILKKGGFTVDVKTGLKPEELKAIIGDYDA
                     LIVRSATKATADIIEAGRNLKVIGRAGAGVDNIDSAAATKRGIVVMNTPGGNTVTTGE
                     HAIALMMSLTRNIPKADAFMKEGKWEKKALEGREVTGKTLGIIGMGRVGSVVASRALG
                     LKMNVIVYDPFITRELAESQGIELVSLDDIYARSDYITVHTPKTKETTGLIGKEAFSK
                     MKKGVFIINCARGGIIDEAALVEALDAGIVAGAAIDVFVKEPPEDWTLAKHPKVVATP
                     HLGASTLEAQENVALAIAEQVVDYLKNGVIRNAVNAPSVSPEMLPKVRPYIELAEKMG
                     MFMGQIIGQVISDRLKKVSIVYTGAASGVDT"
BASE COUNT          308 a          452 c          483 g          218 t
ORIGIN      
        1 acgggtccac atcgagagcg tggcgcgttt ctgggacttc ctcctggcgc tcctcgagag
       61 gctttcggcc aaggcctgct gagaggggcc gggagacccc gcccctccca cggggtggaa
      121 cgaggagttc gcccgcccga gcgggacttc tcccggaagg tgctggaagg cccaccgttc
      181 ccagggtcga cccgggggac cccgccatcc cttcacgcct caaggggtgc acagaccacc
      241 ggtccctcgc acgcagccag gggggatgcc cacgaacgcc cgtgccgtct tgtgttgaca
      301 cctcctgggg cttttggtag aagggcaccg ggttcattcc cattcatccg ccaaaggagg
      361 gggccgtgaa gatcctggta agtgacaagc tgtcggaagc tggggtagac atcctgaaga
      421 aaggcggttt cacagtagac gtcaagacgg gcctcaaacc ggaagaactc aaggccatca
      481 tcggcgacta cgacgcgctc atcgtccgtt cggccacgaa ggcaacggcc gacatcatcg
      541 aggcgggcag gaacctcaag gtgatcggtc gggccggtgc aggcgtcgac aacatagaca
      601 gcgcagcggc gaccaagcgc ggcatcgtgg tcatgaacac gccgggcggc aacacggtga
      661 ccaccggcga acacgccata gccctcatga tgtcgctgac caggaacatc cccaaggcgg
      721 acgccttcat gaaggagggc aagtgggaga agaaggccct cgagggaagg gaagtcacgg
      781 gcaagaccct gggcatcatc ggcatgggcc gcgtggggag cgttgtcgca tcgagggccc
      841 tgggcctcaa gatgaacgtc atcgtgtacg acccgttcat cacgagggag ctcgccgaga
      901 gccagggcat cgagctggtg agcctcgacg acatctacgc ccggtccgac tacatcacgg
      961 tgcacacgcc caagacgaag gaaaccaccg gcctcatcgg caaggaggcc ttctcgaaga
     1021 tgaagaaggg cgtcttcatc atcaactgcg ccaggggcgg gatcatagac gaggcggccc
     1081 tcgtcgaggc gctggatgcg ggcatcgtgg caggcgccgc catagacgtg ttcgtcaagg
     1141 aacctcctga ggactggacc ctggccaagc accccaaggt ggtggccacc ccccacctcg
     1201 gggcgtctac cctcgaggcc caggagaacg tggcgctcgc gatcgccgag caggtggtgg
     1261 actatctcaa gaacggcgtc atccggaacg cggtcaacgc accctctgtg tccccggaga
     1321 tgctcccgaa ggttcggccc tacatcgagc tcgccgagaa gatgggcatg ttcatggggc
     1381 agatcatcgg acaggtgatc tcggacaggc tcaagaaggt gtccatcgtc tataccgggg
     1441 cggcatcagg ggtcgacacg a
//
LOCUS       DAOSCR010000672         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_59549_length_1455_cov_3.823571, whole genome shotgun sequence.
ACCESSION   DAOSCR010000672 DAOSCR010000000
VERSION     DAOSCR010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59549_length_1455_cov_3.823571"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLT69_12645"
     CDS             <1..580
                     /locus_tag="PLT69_12645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ15334.1"
                     /translation="PGILERVMASSDPKVTERLVPVLARMDRDVAGGFLLKLLRHPFG
                     KVRLEVVRQVVRMGLHAKEIFRLIDDEDENVSRMVVGVLGASRSREAERLLMEYLKRR
                     TFGRTEDAHGLSCYEALGKCGSSECISFLRGRLMEKPWMPPFWKRTERLGAALALARL
                     GSAESRKVLEKASRSLWPSLRYIVRKAMEAKR"
     gene            577..>1455
                     /locus_tag="PLT69_12650"
     CDS             577..>1455
                     /locus_tag="PLT69_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ15335.1"
                     /translation="MIGEGERRGGSSQVEDKRMTFLLGERLIVGLYRLIQATRIHHDN
                     NQVVVEALGRCTAALDALLGGGEVPVQVWRDRLYVCGERLPFRKDVAGVMTDLVEFMN
                     ARSLGGIVFSRVAGEDLAGCLLAFVRLLNTAHGHQDPVGWLEDALREKGISCIEVREI
                     QDEEAGTPERNVRKRAVDMYFNALKVVKGVASKMDRGVAGVRKARRVAQEVVDLINED
                     PSIMLALATIRDYDDYTYMHSVNVAILAVSLGRYVGLSRVSLEHLAVCGLFHDLGKIE
                     IPKGILQTQVSHADFWL"
BASE COUNT          269 a          402 c          510 g          274 t
ORIGIN      
        1 ccccgggatc ctcgagcgtg tcatggcgtc gtcagacccg aaggtcacgg agcgtctcgt
       61 gccggttctg gccaggatgg acagggacgt agccggcggt ttcctgttga aactccttcg
      121 acaccccttt ggcaaggttc gtctggaggt ggtgagacag gttgttcgca tgggattgca
      181 tgcgaaggag atcttcaggt tgatcgacga cgaggacgag aacgtcagca ggatggtcgt
      241 cggcgtactg ggcgcctcca ggagcaggga ggcggagagg ctgctcatgg agtacctgaa
      301 gcggcgaacc ttcgggcgca ccgaggacgc tcatgggttg tcctgctacg aggcgctcgg
      361 caagtgcggt tcgtcggaat gcatttcttt cctgaggggg cgattgatgg aaaagccgtg
      421 gatgccgcct ttctggaaga ggaccgagcg gttgggagct gcgctcgcgc tggccaggtt
      481 gggcagtgcg gagtcgagga aggtcctcga gaaggcgtcc aggagtctgt ggccgtctct
      541 caggtatatc gtccgcaagg ctatggaggc gaagcggtga tcggtgaggg agaacgaagg
      601 ggcggctctt cgcaggtgga agacaagcgg atgaccttcc tcctcggtga gcgactgatc
      661 gtgggcctgt accggctcat ccaggcgacg aggatccacc atgacaacaa ccaggtcgtc
      721 gtcgaagccc tcggccgatg cacggcggcg ctcgatgccc tcctgggcgg gggcgaggta
      781 cccgtccagg tgtggcgcga ccggttgtac gtgtgcggtg agaggttgcc tttccggaag
      841 gacgtagccg gcgtcatgac cgacctcgtg gagttcatga acgcccgctc actcggcggt
      901 atcgtcttca gccgcgtcgc cggggaggac ctcgcgggct gcctccttgc tttcgtgagg
      961 cttctcaaca cggcgcacgg tcaccaggac cccgtggggt ggctcgaaga cgctctcagg
     1021 gagaaaggga tttcgtgtat cgaggttcga gagatacagg acgaggaggc cggcaccccg
     1081 gagaggaacg tgcgcaaacg ggccgtcgac atgtatttca acgccctcaa ggtggtcaag
     1141 ggcgtcgcct cgaagatgga caggggggtc gcgggcgtca ggaaggccag gcgtgtcgca
     1201 caggaggtgg tcgatctcat caacgaggac ccctccatca tgctcgccct tgcaaccatc
     1261 agggattacg atgattacac gtacatgcac tcggtcaacg tggccatcct cgccgtatcc
     1321 ctcggcaggt acgtgggcct ctcgagggtc agcctggagc acctggccgt ttgcgggctc
     1381 ttccacgacc tcggaaagat cgagatcccc aaggggatac tccagacgca agtctctcat
     1441 gccgacttct ggctg
//
LOCUS       DAOSCR010000673         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_59634_length_1454_cov_2.369550, whole genome shotgun sequence.
ACCESSION   DAOSCR010000673 DAOSCR010000000
VERSION     DAOSCR010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59634_length_1454_cov_2.369550"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLT69_12655"
     CDS             <1..253
                     /locus_tag="PLT69_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ15336.1"
                     /translation="DVIDSVHLSPSLQMKVVVPGKKKLGQLSSLISAYERSLIVDALK
                     DAKGNQSEAARLLGTTKRIIQYKLDKYGIDPKRFKPRKP"
     gene            947..>1454
                     /locus_tag="PLT69_12660"
     CDS             947..>1454
                     /locus_tag="PLT69_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOJ15337.1"
                     /translation="MCRLFAITSDEPLSPMTAIQALDVMKEGHDGSGVGLLMSDLGGE
                     LEQFKGSPILSGIFTQEGIKRLDRYMMELGLLTKYKMSIRPNTTPPRGTPRRDVYLIR
                     VYEYPYEWEGLSQDEIEQRLLAIRLDLRRMGEDDGSMMVFSFWPDTIMIKEVGDPMAV
                     AGYLGLDRQ"
BASE COUNT          292 a          450 c          479 g          233 t
ORIGIN      
        1 ggatgtcatc gacagcgtgc acctgtcgcc ctcgctccag atgaaggtgg tcgtgcccgg
       61 gaagaagaag ctgggccagc tctccagtct gatcagtgcc tatgagcgct cgctgatcgt
      121 ggacgcgctc aaggacgcga aaggaaacca gagcgaggcg gcgagactcc tcgggaccac
      181 caagcgcatc atccagtaca agctcgacaa gtacgggata gacccgaaga ggttcaagcc
      241 aaggaagccg tgatgcaagc ccgggcctgg agctggcacc ctgggcgcgg tccgtgcgtc
      301 gagggggtcg aggcatcccc ccggtgagcc gacggcatcc cggccgcacc cgtcacacgg
      361 gcgctgagcg agccccgatg gaccggttca ccccagaccc gccgtcctcg attgtcgccg
      421 agctgaagag gcccccggct tctgcgtgac ccgcatccct gcggacccat cccacggtga
      481 gactgcacgc acaaagtggt ttgtatcgcg gccggtttcc cgtaagctgt ggtctcgcct
      541 gtcttgcgaa ggcgggtcaa gaaacgtgat cgacacgagg agccctgccg tcacccccag
      601 ggacgtgcag gccccccccc gggggatggc cggccgccat ggagaatgca gggctgcgag
      661 cggtcctgcc cgggcgccgg gccgatcatc gggcgggaaa gaggcgcccc gggggtttca
      721 atggcacaaa agtgttccat ttcgatcgta tgtgacgata aggtgccacc tggcaccgac
      781 ccccggccat gaagaccggc ggacccgtcg taccgggtct tccgggcgtc atgcaagcgc
      841 gctcgcctct ttctcgctgg agtaaaaaag acggcatcgg gggtttgggt gcgtggcacg
      901 cttctggcta atacccccac cgatcgaaca tggaggtagc aagaacgtgt gccgtttgtt
      961 cgccatcacg agcgacgaac ccctctcccc catgacggct atacaggccc tggacgtcat
     1021 gaaggaagga cacgacgggt ccggcgtcgg gctcctgatg tccgacctgg ggggcgagct
     1081 cgaacagttc aaggggtcgc ccatcctcag cgggatcttc acgcaggagg gcatcaagag
     1141 gctcgacagg tacatgatgg agctcggcct cctgacgaag tacaagatgt ccataaggcc
     1201 gaacacgaca ccccccaggg ggaccccgag gcgtgacgtg tacctcatac gcgtctacga
     1261 gtacccgtac gagtgggaag ggttgagcca ggacgagatc gaacaaaggc tcttggccat
     1321 acgcctggat ttgaggagga tgggcgagga cgacggatcc atgatggtct tctcgttctg
     1381 gccggacacc atcatgatca aggaggtggg cgacccgatg gcggtggccg gctaccttgg
     1441 actcgaccgc cagg
//
LOCUS       DAOSCR010000674         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_59683_length_1453_cov_2.917740, whole genome shotgun sequence.
ACCESSION   DAOSCR010000674 DAOSCR010000000
VERSION     DAOSCR010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59683_length_1453_cov_2.917740"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLT69_12665"
     CDS             <1..824
                     /locus_tag="PLT69_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ15338.1"
                     /translation="RMPAMEGTEFLARVRSMSPDTVRIMLTGYADLATAMDAINRCGV
                     YKFIEKPWRNAELVGVVKESLERHRIVTVMRDSDEPALLSLASAIELKDPYTKGHCDS
                     VAGYAVAVGKAIGMPENEVRQMKYGCWLHDCGKIGVPEAILNKPGPLTEEEFEIVRKH
                     PEWGAELARQARLSEIVVNVIACHHERFDGTGYPRGLKAGAIPIHARIACIADVFDAL
                     TSDRSYRARHSHVEAVGMLYSMKGSFFDPELAEVFLTLVAEEVAGEKTAGDQDGE"
     gene            814..>1453
                     /locus_tag="PLT69_12670"
     CDS             814..>1453
                     /locus_tag="PLT69_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ15339.1"
                     /translation="MGSEGTRQRTEVLFVDDEENILKALSRLFMDEDFTVRTAASGQA
                     GLEILEASGSIGVVVSDHRMPGMSGVEFLTRVKERKPETLRILLTGYGDIATIKDALN
                     KAGACRYITKPWVDDELVQAVREAVVRFELTRENARMAEVIRAQNEQLKEWNSQLEIM
                     VQEQTIELTRQNEKLKALLQRERATIRGVIGALSGLIELRNKRVRNHSRNVAE"
BASE COUNT          300 a          434 c          510 g          209 t
ORIGIN      
        1 accggatgcc cgccatggag ggcaccgagt tcctcgccag ggtcagatcc atgagcccgg
       61 acacggtgag gatcatgctg accggttacg ccgacctggc gacggccatg gacgccatca
      121 accgctgcgg ggtctataag ttcatcgaga agccatggcg gaacgcggag ctcgtgggtg
      181 tggtcaagga gtccctggag aggcacagga tcgtcacggt gatgcgtgac tcagacgagc
      241 cggcactgct gtcgctggcc agcgccatcg agctcaagga cccgtacacc aaggggcact
      301 gcgacagcgt ggccggctac gccgtcgcgg tgggcaaggc gatcggcatg cccgaaaacg
      361 aggtcaggca gatgaaatac ggctgctggc tccacgactg cgggaagata ggggtgcccg
      421 aggcgatcct gaacaagccc ggcccgctca ccgaggagga gttcgagatc gtgaggaaac
      481 accccgagtg gggcgccgag ctcgccaggc aggcgcggct gtcggagatc gtcgtgaacg
      541 tcatcgcctg ccaccacgag cggttcgacg gtacggggta cccgaggggg ttgaaagcgg
      601 gcgccatccc catacatgcc cgcatcgcgt gcatcgcgga cgtcttcgac gccctcacct
      661 cggaccgctc ctaccgcgcg aggcacagtc acgtggaggc ggtgggcatg ctctattcca
      721 tgaaaggctc cttctttgac ccggagctcg cggaggtgtt cctcaccctc gtggctgaag
      781 aagtcgccgg ggagaagacg gcgggggatc aggatgggga gtgaaggaac ccggcaaagg
      841 acagaggtcc tcttcgtaga tgacgaggag aacatcctga aggcgctctc caggctgttc
      901 atggacgagg acttcacggt caggaccgcg gcctccggcc aggccgggct cgagatcctc
      961 gaggcgagcg ggtctatcgg ggtggtcgtc tccgaccaca ggatgccggg catgagcggc
     1021 gtggaattcc tgacccgcgt caaggaacgc aagccggaga ccctgcgcat cctgctgacg
     1081 ggttacgggg acatcgcgac gatcaaggac gcgctcaaca aggcgggcgc ctgtaggtac
     1141 atcaccaagc cctgggtcga cgacgagctg gtgcaggcgg tgcgcgaggc ggtggtgcga
     1201 ttcgagctca cccgcgagaa cgcgcgcatg gccgaggtca tcagggccca gaacgagcag
     1261 ctgaaggagt ggaactccca gctcgagatc atggtccagg aacagaccat cgagctgacg
     1321 aggcagaacg agaagctcaa ggccctcctc cagcgcgagc gggccaccat caggggcgtg
     1381 atcggcgcct tgtcagggct catcgagctc aggaacaagc gggtgaggaa ccactcgcga
     1441 aacgtcgccg agc
//
LOCUS       DAOSCR010000675         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_60320_length_1442_cov_3.437635, whole genome shotgun sequence.
ACCESSION   DAOSCR010000675 DAOSCR010000000
VERSION     DAOSCR010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60320_length_1442_cov_3.437635"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..874
                     /locus_tag="PLT69_12675"
     CDS             164..874
                     /locus_tag="PLT69_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOJ15340.1"
                     /translation="MRKIHAVLLVAFASLLLQACSHSTMDALREKEFTEDEIYPPVDE
                     TGEGVKSPGSLWSENAKINDMFSDSRARRVGDIVTVRIIESSSADKEAKTEASRTSST
                     DHSVSSLLGLPLDQSSVFGYKLKPEVSTDSSSEFEGDGKTSRRGTLTAVVPARITRVL
                     PSGNYLIKGKKQIRVNDEAQYIVVSGMIRPDDILWNNSVLSTTIADLKVDYYGKGILG
                     DQQGKGFLARVIDKVWPF"
     gene            885..>1442
                     /locus_tag="PLT69_12680"
     CDS             885..>1442
                     /locus_tag="PLT69_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOJ15341.1"
                     /translation="MSRKAVVLACMLVCVTNAAWAVRVKDLVQIQGVRENKLVGYGLV
                     VGLNGTGDKAGTSTQYTVNALANMLRNMGILVDPEEIKMKNVAAVIVTSSLPPFAKSS
                     QKLDCVVSSIGDATSLAGGTLVATPLMGPDGKVYAIAQGPISVGGFAVAGASGTGVTK
                     NFPTVGTIPNGAVVERTMEATLGPNL"
BASE COUNT          283 a          409 c          518 g          232 t
ORIGIN      
        1 ggtcgcggag gccggcggcg tggaggtgga ggcgaaaggg gtggcggtca gggacgcccg
       61 caccgggcag agcgtgaagg tgaagaacct cgcatcaggc cggctcgtcg ttggcaccgt
      121 tattgctcag gatctggtag aggtcgggct gtaggaggaa gacatgagga agatacatgc
      181 ggtactgctg gtcgcctttg cctccttgct ccttcaggcg tgctcgcaca gcaccatgga
      241 cgccctgagg gagaaggagt tcaccgagga cgagatctac ccccccgtgg acgagacagg
      301 agaaggcgtc aagagtcccg gctcgctgtg gtccgagaac gccaagatca acgacatgtt
      361 cagcgacagc agggcgcggc gcgtgggcga catcgtcacg gtgaggatca tagagagctc
      421 gtccgcagac aaggaggcca agaccgaggc gtcgaggacg agcagcaccg accactcggt
      481 ctcgagctta ctcgggcttc cgctcgacca gtcgtcggtc ttcgggtaca agctcaagcc
      541 cgaggtgtcc acggacagct cttccgagtt cgagggggac gggaagacct cgaggagggg
      601 taccctcacg gcggtcgtcc cggcgcgcat cacgagggtg ctcccctccg ggaactacct
      661 gatcaagggg aagaagcaga tccgggtcaa cgacgaggcc cagtacatcg tggtgtcggg
      721 catgatcagg cccgacgaca tcctgtggaa caactcggtt ttgtccacca ccattgcgga
      781 cctgaaggtg gactactacg gcaaggggat cctgggcgac cagcagggca aggggttcct
      841 ggcccgggtc atcgacaagg tctggccgtt ctagggggtg tgtcatgtcg agaaaggccg
      901 tcgttctggc ttgcatgctg gtatgcgtga cgaacgccgc ctgggcggtg cgggtgaagg
      961 atctcgtcca gatccagggg gtgcgtgaga acaagctcgt ggggtacggg ctcgtggtcg
     1021 gcctcaacgg gacgggcgac aaggcgggaa cgagcaccca gtacacggtc aacgcgctgg
     1081 ccaacatgct caggaacatg ggcatactgg tcgatcccga ggagataaag atgaagaacg
     1141 tggccgcggt gatcgtcacc tcgtccctgc cgccgtttgc caagagctcg cagaagctcg
     1201 actgcgtggt gtcttccata ggggacgcca catcgctcgc cggggggacg ctcgtcgcca
     1261 cgccgttgat ggggcctgac gggaaggtct atgccatcgc gcagggaccg atctcggtgg
     1321 gagggtttgc cgtggcgggg gcctccggca ccggggtcac caagaacttc cccacggtgg
     1381 gcaccatccc gaacggggcg gtggtcgagc ggacgatgga ggccaccctc gggccgaacc
     1441 tc
//
LOCUS       DAOSCR010000676         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_60876_length_1433_cov_3.214804, whole genome shotgun sequence.
ACCESSION   DAOSCR010000676 DAOSCR010000000
VERSION     DAOSCR010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60876_length_1433_cov_3.214804"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLT69_12685"
     CDS             complement(<1..969)
                     /locus_tag="PLT69_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ15342.1"
                     /translation="MKHVELALKLAEEISGLEGTGARLPTVREVARREGVSLVTAQRA
                     YRHLSDLGIVVSRRGSGTFAAEPIGRGPIDLCGIRVGEEQLRWVLPYLRGDLDGLCTY
                     DPPEGYGPLRESAGAWLARMGVRGDPIVTSGSQQAIFLVGMACLKQGDVVVVEEPGYQ
                     GAARIFRSLGASVRAVPYLAHPRDVDALAALEPRLFYTMPQGHIPTGATMPAEVRLRL
                     LERAAGSGFLVVEDDPFSDILGVSPLKSMDEQEVVVHLRSLSNILGPGLRMGITVAPR
                     AMRDRLVQLKEINDLSISGVLQRILCAIFSSDAFAGHLRALAKELPL"
     gene            1063..>1433
                     /locus_tag="PLT69_12690"
     CDS             1063..>1433
                     /locus_tag="PLT69_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOJ15343.1"
                     /translation="MEQDDRVMVNRGLATMLVGGVIMDVTNVEQARIAEDAGAVAVMA
                     LERVPAHIRAAGGVARMTDPEIVLGIKEAVSIPVMAKVRIGHFMEARVLEALEVDFID
                     ESEVLTPADEEHHIDKRAFRVP"
BASE COUNT          258 a          454 c          490 g          231 t
ORIGIN      
        1 aaggggcaac tccttggcaa gggctcgcag gtgtccggcg aaggcgtccg acgagaagat
       61 cgcgcagagg atcctctgca gcacgcccga gatcgagagg tcgttgatct ccttgagctg
      121 cacgagacgg tcgcgcatcg cgcgcggggc gaccgtgata cccatgcgga ggcccggacc
      181 caggatgttc gaaaggcttc tcaggtggac gaccacctcc tgctcgtcca tggacttgag
      241 gggggacacg cccaggatat ccgagaaggg gtcgtcctcg accacgagga aacccgatcc
      301 tgccgccctt tcgaggagcc gaaggcggac ttcggctggc atggtggcgc ccgtggggat
      361 gtgtccctgg ggcatcgtgt agaagagtct cggctcgagg gcggccaagg cgtccacatc
      421 ccgggggtgg gcgaggtacg ggaccgccct caccgaggcc ccgagtgacc tgaagatccg
      481 tgcggcaccc tggtagccgg gttcctcgac caccacgacg tcgccttgct tgaggcaggc
      541 catcccgacg aggaagatgg cctgctggct gcctgaggtg acgatcgggt cccccctgac
      601 gcccatcctg gcgagccagg cccccgcgga ctccctgagc ggcccgtagc cctcgggcgg
      661 gtcgtacgtg cacagcccgt cgaggtcacc cctgagatac ggcaggaccc atctcagctg
      721 ttcctctccc acccggatgc cgcacaggtc gatgggtccg cgtccgatag gctcggccgc
      781 gaaggtgccc gaacccctcc tcgagacgac gatcccgagg tcggagagat gcctgtacgc
      841 cctctgtgcc gtgacgaggc tcaccccctc cctccgggcc acctcgcgga ccgtcggcag
      901 cctcgctccc gtcccttcca ggcccgagat ctcctccgcg agcttgagcg cgagttctac
      961 gtgcttcatg caggtcgccc cttgactggc atgaacagac gtgtaacatt catataacaa
     1021 aaagtcaatt gttatataac aattgaccag ggggtgggag tcatggagca ggacgatcgc
     1081 gtcatggtca acagggggct cgccacgatg ctggtgggcg gcgtgatcat ggacgtcacg
     1141 aacgtggagc aggcgcgcat cgcagaggac gcgggggcgg tggccgtcat ggcgctcgag
     1201 cgggtgcccg cgcacatacg cgccgcaggc ggggtcgccc ggatgaccga cccggagatt
     1261 gtcctgggga tcaaggaggc ggtgagcatc cctgtcatgg ccaaggtgag gatcgggcac
     1321 ttcatggagg cgcgtgtcct cgaggcgctc gaggtggact tcatcgacga gagcgaggtg
     1381 ttgacgcccg cggacgagga acaccacata gacaagaggg cgtttcgggt ccc
//
LOCUS       DAOSCR010000677         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_61517_length_1423_cov_3.202485, whole genome shotgun sequence.
ACCESSION   DAOSCR010000677 DAOSCR010000000
VERSION     DAOSCR010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61517_length_1423_cov_3.202485"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..846)
                     /gene="hisH"
                     /locus_tag="PLT69_12695"
     CDS             complement(235..846)
                     /gene="hisH"
                     /locus_tag="PLT69_12695"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOJ15344.1"
                     /translation="MIAVIDYRAGNLRSVERALLHVGARCTVTAEHERILEADRVVFP
                     GVGAAGAAMETIRESGLDAVIREVVARKTPFLGICLGTQVIFETSEEDDAACLGILRG
                     TVKRFPEGCGKIPHMGWNTLKLVRDHPLLSGVGRDSWFYFVHSYYPEPSDATDVVART
                     SYGIEFASVVARSNVFATQFHPEKSGEPGLAILRNFCAWDGRE"
     gene            complement(886..1305)
                     /locus_tag="PLT69_12700"
     CDS             complement(886..1305)
                     /locus_tag="PLT69_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15345.1"
                     /translation="MKLVKAVLPAVLLVTCLALPGCDTDRDEIVAPIRLEMPTDDLQK
                     EIGVSGFILGTFIMLNGYLVIIASCIVLLVKESTFSKILDLVRMAAPMIGSGERPGGG
                     AESLLAPLTDAATRVKVGYIGLLAGLVLAYIGAWIAL"
BASE COUNT          247 a          497 c          444 g          235 t
ORIGIN      
        1 ccgggtcaac ccacgggcgc atgccctgtg ccagcccgtc ttgcgacacg ccggggacgg
       61 accttagcgc aggccgcacg tcgaggggtt ccgccgacag gcgtgcttgc acggctctct
      121 catccggcgc gggaagcacc cacgcatgcg gcccagggcg gcaagccgag gcagggtatc
      181 gacccccgcc cgttcgtccg ccacgacccc tttgccgcct tcgccgtcca agccctattc
      241 cctgccgtcc cacgcgcaga agttcctcag gatggccagg cccggctcgc cgctcttctc
      301 ggggtggaac tgggtggcga acacgttgga acgggccacc acgctcgcga actcgatccc
      361 gtacgacgtc ctcgccacca cgtcggtcgc gtccgatggc tcggggtagt aggaatggac
      421 gaagtagaac cacgagtccc gccccacgcc tgacaagagc gggtggtcgc ggacgagctt
      481 caaggtgttc cagcccatgt gggggatctt tccgcaccct tcggggaacc tcttcaccgt
      541 tcccctcagg atgccgaggc aggctgcatc gtcttcctcg ctcgtctcga agatgacctg
      601 ggtccccagg cagatgccga ggaatggggt cttccttgcc accacctccc gtatgaccgc
      661 atccagcccg gattccctta tggtttccat cgcggccccc gcggcgccga ccccggggaa
      721 gaccaccctg tcggcctcca gaatccgctc gtgctccgcc gtcaccgtgc agcgggcgcc
      781 gacgtgcagg agcgcgcgct ccaccgacct caagtttcct gcacgataat cgatgacggc
      841 gatcatgcga cgggcgatgc ccggccccca cgcgggggcc gaaccctaca aggctatcca
      901 ggcccctatg taggcgagca cgaggcctgc aagcagcccg atgtaaccca ccttgaccct
      961 cgtcgccgcg tccgtcaggg gcgcgagcag ggactcggcc cctcccccgg gacgttcccc
     1021 ggacccgatc atgggcgcgg ccatgcgcac gaggtcgagg atcttcgaga aggtcgattc
     1081 cttgaccaaa aggacgatgc agctcgcgat gatgacgagg tagccgttga gcatgatgaa
     1141 cgttccgagg atgaagcccg agacgcctat ctccttctgg aggtcgtcgg tgggcatctc
     1201 gaggcgtatg ggggcgacga tctcgtcacg gtcggtgtcg cacccgggaa gtgcgaggca
     1261 tgtcaccagc agaacagccg gaaggactgc tttgaccagc ttcaccacaa gggcctccag
     1321 atcgggtttg ctggagacta tacccgcagg ctcgagttcg tgtcaacacc ctcacccctg
     1381 cggtgacgag acggatcttg cctccgggaa ggaggaggcg cct
//
LOCUS       DAOSCR010000678         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_61973_length_1416_cov_2.572373, whole genome shotgun sequence.
ACCESSION   DAOSCR010000678 DAOSCR010000000
VERSION     DAOSCR010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61973_length_1416_cov_2.572373"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..1251)
                     /locus_tag="PLT69_12705"
     CDS             complement(415..1251)
                     /locus_tag="PLT69_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ15346.1"
                     /translation="MRFRPLAGFCEIRTTFAAATVCTGAQAPHLMHGRGIAMHDMKSG
                     KVFRYSVLLAAVLVMTGCSLLSVPVIRNASRNDVVNLGDSIFALSGKILDYLHSWSGQ
                     TFRRYALSGSMIVHIEDQYSDAKADNPNIATIFMDGGGNDILIPATLFDPYNCKVDWW
                     ESGLSSTCKALINDIYVDVVDLLNQMGRDGVDNIIYLGYYRLKNGLIGSTSLNPAVDY
                     GDAKLALAVQNATAVPNYRVFIDPRSSITSSDIIYDGVHPSDSGSMKLATLIWAKLQP
                     LL"
BASE COUNT          240 a          438 c          490 g          248 t
ORIGIN      
        1 ccatggagga ccccagcggt gatcaggata ccctcaggcg ccgcctgatg caaccgtcgc
       61 cggcctgcga cgcgcaggat agcccttgac gtcacgctct cccctgtcgc tcgcgcatgg
      121 cggcctcgcg agggcggctg cacgggagac gggaaagtcc cgggcgaagg ccttgggcgc
      181 aaaggcccgc cgcgccctgc gggcgccgcc gggaaagggc ggaggcagtg cccggggtga
      241 ggcggctttc ccgtcgccgc ccggccccgg cgggagcgag cagccgttct ctgtcggccg
      301 ggccctcccg gccgagcgcg tgcatcgaga cccctcggcc gtgaagcccg cttcacgcgg
      361 ggcggcgcag gatacgggga gcggggtctc tgcgaacccg ctcccccatg ccttctacag
      421 gagcggctgg agtttggccc agatgagggt ggccagcttc atggaccccg agtcgctcgg
      481 atggacaccg tcgtagatga tgtcggagct cgttatggat gaccgtgggt cgatgaaaac
      541 cctgtagttc gggacggccg tggcgttctg gaccgcgagg gcgagcttgg cgtccccgta
      601 gtccaccgcg gggttgagcg aggtgctgcc gatgagcccg ttcttgagcc ggtagtaccc
      661 gaggtagatg atgttgtcca ccccgtccct ccccatctgg ttgaggaggt cgacgacgtc
      721 cacgtagatg tcgttgatga gcgccttgca cgtcgaggag agaccggact cccaccagtc
      781 caccttgcag ttgtacgggt cgaacagcgt ggcgggtatg aggatgtcgt tcccgccgcc
      841 gtccatgaag atggtggcga tgttcgggtt gtccgccttt gcgtcggagt actggtcctc
      901 gatgtggacg atcatggagc cggacagggc gtaacgcctg aacgtctggc ccgaccacga
      961 gtggaggtag tccaggatct tgccggacag cgcgaagatg gaatccccga ggttcaccac
     1021 gtcgttgcgg ctcgcgttcc tgatgacagg cacgctgagg agcgagcacc cggtcatcac
     1081 gaggacagcc gcgagcagga cggaatacct gaaaaccttc cctgacttca tgtcgtgcat
     1141 tgcaatcccc cttccatgca tgagatgggg cgcctgggcg cccgtgcata ccgtggcggc
     1201 ggcaaaggtc gtcctgatct cgcagaaacc cgccaggggg cggaacctca tggaattatt
     1261 gtcttacata tatgacaatc atgggggtgc gtcaatggac atcgtgtaaa acttgggtaa
     1321 agcggcacag gcgcctgggc agggggtggc ggcggtctct cggcccttcg ccccgttcca
     1381 tcgacacaag ggcggcacct cgccgagacc gtgccc
//
LOCUS       DAOSCR010000679         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_62410_length_1409_cov_2.395864, whole genome shotgun sequence.
ACCESSION   DAOSCR010000679 DAOSCR010000000
VERSION     DAOSCR010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62410_length_1409_cov_2.395864"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1086
                     /locus_tag="PLT69_12710"
     CDS             280..1086
                     /locus_tag="PLT69_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ15347.1"
                     /translation="MILAATAMAAPAEAARKNLVLATTTSTQDSGLLDVLIPIFEKKT
                     GYFVKTIAVGSGQAMAMGSKGEADVLLVHSSAAEKKFMAEGYGVSRKLVMHNDFVIVG
                     PSADPAGIKGMKSAAEALKRIASRGMVFVSRGDSSGTHAKETDLWKAAGIDPEGRRYY
                     QQTGLGMGQTLWVAAEKKAYTLTDRGTYLALKEKLGLDILVEKDKGLLNVYHVIEVNP
                     ARFPRVNAAAAKAFTDFMVGKEAQAIIADFGMDTYGSPLFFPDAGKPEYE"
     gene            1145..>1409
                     /locus_tag="PLT69_12715"
     CDS             1145..>1409
                     /locus_tag="PLT69_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate transporter permease"
                     /protein_id="HOJ15348.1"
                     /translation="MELIVEGVRKAILLLVTFDPEVYGITWLSLKVSGTATFISLIIG
                     ISLGTVVALSRFPGRGLVVSLINTGMGVPPVVVGLFVTIFLWRN"
BASE COUNT          272 a          440 c          480 g          217 t
ORIGIN      
        1 ggtccgtacc ctgaggggat acagcgaacg ggacaccggc agggtcatgt atccacggca
       61 ggacgggtcg agaccgccac ggcacgacac cttgagcccc cactgagcgc cggggtgttt
      121 tcgtgcatgc aggggttcca tgcaacaggg ttggaagacg agacgggatc ggaagaagac
      181 aggggaggca acgagcgcat gatgggccgc aggcaagggg tcgagagacg agtgcatatc
      241 ccgaggggag gcttcccgtc gcctgcatgg atcttggtca tgatccttgc tgcgacggcg
      301 atggccgccc ccgcggaggc ggcccggaag aacctcgtcc tggccaccac cacgagcacg
      361 caggactcgg gcctcctgga cgtgctcatc cccatcttcg agaagaagac cgggtacttc
      421 gtgaagacca tcgccgtggg ctcgggccag gccatggcca tgggctccaa gggcgaggcg
      481 gacgtcctgc tcgtgcactc ctcggccgcg gagaagaagt tcatggccga gggctacggc
      541 gtgagccgga agctcgtcat gcacaacgac ttcgtcatcg tgggcccgag cgccgaccct
      601 gcgggcatca agggcatgaa gtccgccgcc gaggccctca agcggatcgc gtccaggggc
      661 atggtgttcg tctcccgggg tgacagctcg ggcacccacg ccaaggagac ggacctctgg
      721 aaggcggcag gcatcgaccc cgagggccgg aggtactacc agcagaccgg gctcggcatg
      781 ggacagacgc tctgggttgc cgccgagaag aaggcctaca ccctcacgga ccgcggcacc
      841 tacctggctc tcaaggagaa gctgggcctg gatatcttgg tggagaaaga caaggggctc
      901 ctcaacgtct accacgtgat cgaggtgaac cccgccaggt tccccagggt gaacgcagcc
      961 gcagcgaagg ccttcaccga cttcatggtg ggcaaggagg cgcaggcgat catcgccgac
     1021 ttcggcatgg acacctatgg cagccccctc ttcttcccgg atgccggcaa gcccgaatac
     1081 gagtaagcaa aacaggggag ggcggggatg accccaccct ccccgccgcc atgaggacag
     1141 gaccgtggaa ctgatcgtag aaggcgtcag gaaagcgatt ctcctcctgg tcacgttcga
     1201 cccggaggtg tacgggatca cctggctgtc gctcaaggtg tccgggaccg ccaccttcat
     1261 cagcctgatc atcggcatct cgctcgggac cgtggtggcg ctcagccgtt ttcccgggcg
     1321 cgggctcgtg gtgagcctca tcaacacggg catgggcgtg ccgcccgtgg tggtgggcct
     1381 gttcgtgacc atcttcctgt ggcgcaacg
//
LOCUS       DAOSCR010000680         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_62583_length_1406_cov_2.741673, whole genome shotgun sequence.
ACCESSION   DAOSCR010000680 DAOSCR010000000
VERSION     DAOSCR010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62583_length_1406_cov_2.741673"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLT69_12720"
     CDS             <1..454
                     /locus_tag="PLT69_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOJ15349.1"
                     /translation="SQYMNQHYNLDDMDYGDLAAKVKLIAIDEMRHAEIFAERIKELG
                     GEPTTEPAAKVERGQKVEAISPFDAGLEDDTIDVYNTFIQVCRESGDSISMKLFETVI
                     AEEQVHFNYFDNVTEHIKSLGAAYLSQIAGTPAETGGFTKGFVTQQGA"
     gene            complement(855..>1406)
                     /locus_tag="PLT69_12725"
     CDS             complement(855..>1406)
                     /locus_tag="PLT69_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOJ15350.1"
                     /translation="DRMARENVKLFKEKGVKKIITLCPHCFSTLANDYRQYGFEGEVV
                     HHADLIDSLIKQGRLKLKKKVDAGRVVFHDSCYLGRHNDIYESPRSVISQATGKAPIE
                     MDRNRAESFCCGAGGGRMWMEEHLGTRINLERVGEALTKNPDTICVTCPYCMTMFDDG
                     LKDKNADKVMVKDIAEVVAEALQ"
BASE COUNT          250 a          452 c          439 g          265 t
ORIGIN      
        1 aagccagtac atgaaccagc actacaacct cgacgatatg gactatggcg acctcgccgc
       61 caaggtgaag ctcatagcga tcgacgagat gcgccacgcc gagattttcg ccgagaggat
      121 caaggaactc gggggtgaac ccacgacaga gccggccgcc aaggtggagc ggggccagaa
      181 ggtggaggcg atctccccct tcgacgccgg tctcgaggac gacaccatcg acgtgtacaa
      241 caccttcatc caggtgtgca gggagagcgg cgacagtatc agcatgaagc tgttcgagac
      301 cgtcatcgcc gaggagcagg tccatttcaa ctacttcgac aacgtcacgg agcacatcaa
      361 gagcctcggc gcggcatacc tgtcccagat tgccgggacc ccggcggaaa cgggagggtt
      421 caccaagggc ttcgtcaccc agcagggcgc ctgacagcgg cccttcgccc gtcatgggcc
      481 cccggccggc ccctcgaggg tgctggccgc cgccctgttt tggcgtcgat tatggcgcgc
      541 tccgaagcgg ccccttgagg gtgcggcccg ccgcgaggtt cggggatacc cggcgccgat
      601 gaccgggcat acatgcactg cggacacgag ctgtggccgt accgagggag accgcctgcg
      661 cctcgcccct ccgatggggc tgaacgggat ctccccggca ccttgacccc ctcgctcgag
      721 acaggggccc cgatatgcga agctccccct aggggccctt gcctgcgttc ttcttggctc
      781 gagatgggag aggagatatg cgaaggcccc gggtgataca cccggggcct tcctggcttg
      841 atttggggat gcgcctactg gagcgcctct gcaacgacct ccgctatgtc cttcaccatc
      901 accttgtccg cgttcttgtc cttgagaccg tcgtcgaaca tggtcatgca gtagggacag
      961 gtcacacaga tcgtgtccgg gttcttcgtc agggcctcgc caacgcgttc cagattgatg
     1021 cgggtgccga ggtgctcttc catccacatc ctgccgccgc ctgcgccgca gcagaacgac
     1081 tctgccctgt tgcgatccat ctcgatgggg gccttgccgg tcgcctggct gatcaccgag
     1141 cggggagact cgtagatgtc gttgtggcgg ccgaggtaac aggagtcgtg gaagaccacc
     1201 ctgcctgcat ccaccttctt cttgagcttg agccgtccct gcttgatcag gctgtcgatg
     1261 aggtccgcgt ggtggacgac ctcgccctcg aagccgtact gccggtaatc gttggcgagg
     1321 gtggagaagc agtgggggca gagcgtgatg atcttcttca cgcccttttc cttgaagagc
     1381 ttcacgttct ccctcgccat cctgtc
//
LOCUS       DAOSCR010000681         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_62920_length_1401_cov_2.287519, whole genome shotgun sequence.
ACCESSION   DAOSCR010000681 DAOSCR010000000
VERSION     DAOSCR010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62920_length_1401_cov_2.287519"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLT69_12730"
     CDS             <1..447
                     /locus_tag="PLT69_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15351.1"
                     /translation="GALVDVGLIYKVGSFQVSGRQGTLQAGLSMNNLVGNDLGDAADI
                     DEHLDVGFASRIGDDTVIALDYVDILGEVGEDDSVGKRIHLGVEYTPKTGLKLRLGLN
                     QGYPTFGVGLSTGKVKLDLATYAEERGTYAGQEHDRRYIIRLGFSL"
     gene            complement(1103..>1401)
                     /locus_tag="PLT69_12735"
     CDS             complement(1103..>1401)
                     /locus_tag="PLT69_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15352.1"
                     /translation="ATVQFAPGDTMTFTVRADDGDFDMQTLVWTEYNADTDQILNGPG
                     EVDLPEQEGQIMTYRYALTRTVTGPAGNRRMELQVKDARGNLSNAYPIEYLVTD"
BASE COUNT          231 a          495 c          440 g          235 t
ORIGIN      
        1 ggcgcgctcg tggacgtagg tctcatctac aaggtcgggt cattccaggt gtcgggcagg
       61 cagggcacac tccaggccgg cttgagcatg aacaacctcg tcggcaatga cctgggagac
      121 gcagccgaca tagatgaaca cctcgacgtc ggcttcgcat cccgcatcgg cgacgacacc
      181 gtcatcgcac tcgattacgt ggacatcctc ggcgaggtag gcgaagacga cagcgtgggc
      241 aagcggatac acctgggcgt cgagtacacg ccgaagaccg ggctcaagct caggctcggg
      301 ctcaaccagg gctaccccac gttcggcgtg ggtctctcga ccgggaaggt gaagctcgac
      361 ctggcgacct acgcagagga gaggggcacc tatgccggcc aggagcacga caggcgatac
      421 atcatcaggc tcgggttctc cctctgacac ctcgacccgt cccggccggg cccgccgtcc
      481 ctgaacggga cgcacagacg ccggaggccg gtcccgaggc tcgcaccccg atacgcccac
      541 gtggcccggc acgagcctca ggccgcatga tgaggaacgc aggtgtcgag ccctgcctcg
      601 cccacgggcc caccccctcg agccgccgcc gggctgccac gttccctcga cgcgcatccg
      661 ggatgcgagg aaaaccgacc tttggcactc gcgaagcggg gcaatccttt cttgggccgt
      721 tcacacgaac cctgcaggca catcccgagg gcaacgcacc aggctgccgg gcttcgcctg
      781 cgcgcaccct ggagagggtg caccttcgtc accctctcac gaccgccgag cccccctgat
      841 ccgggcccat cccggcccgg ccgcttcccc acgtgcgccc tgcatcttcc ccggcaggca
      901 ctcccgagag accgggcgcc gcattatggg ggcccgacgt cgcctaggga gcgatgggcg
      961 tctttgcggt ttcctacatc ctcatggcgg gcggcacggt ctttctgcaa gggccttcga
     1021 ggcggttacg gtgcgcacgc cgactcgcac caccctgcag gtgccgtggg tttccactga
     1081 atcgccgacg aggcggggac ggtcaatcgg tcaccaggta ctcgatgggg tatgcgttgc
     1141 tcaggttccc ccgcgcgtcc ttgacctgca gctccatcct cctgttcccg gctggccccg
     1201 tcacggtcct ggtcaacgcg tacctgtacg tcatgatctg gccttcttgt tcaggaaggt
     1261 ccacctcccc cgggccgttc aggatctggt ccgtgtcggc gttgtactcg gtccacacga
     1321 gcgtctgcat gtcgaaatct ccgtcgtcgg cgcggaccgt gaacgtcatg gtgtcgccgg
     1381 gtgcgaactg gaccgtggca t
//
LOCUS       DAOSCR010000682         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_62954_length_1400_cov_3.524164, whole genome shotgun sequence.
ACCESSION   DAOSCR010000682 DAOSCR010000000
VERSION     DAOSCR010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62954_length_1400_cov_3.524164"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLT69_12740"
     CDS             <1..772
                     /locus_tag="PLT69_12740"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HOJ15353.1"
                     /translation="PEIHVVRSEELKNTSAAGTAATQGAAGTPSNLPTGRGGPEAAPG
                     EAGRNAGSIVRNYEIGRNQTESIFSPGEIKRLTVSVVVDGTYKTDKDGNKLFVARQAS
                     ELKEIENAVKNAVGFNADREDLISVSCMPFAQDEVDLGSLSKEKNKELIMTAARYALF
                     FVLVLLVLLLVVRPLLNWLTKSVRVVEHVHAREREVGYEEPAMIEGSEGVPRIEASAK
                     SEEMKRAVQGKRKTIENITKNDMNTATAVVKTWLQESS"
     gene            769..1374
                     /locus_tag="PLT69_12745"
     CDS             769..1374
                     /locus_tag="PLT69_12745"
                     /inference="COORDINATES: protein motif:HMM:NF026192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15354.1"
                     /translation="MTLKTKKMDGPQKAAIFLMAMGDDFTREVIKGLNEHEVTLLGRR
                     MAELENATIPVDTVRTIMDEFEQLSNEVSGVTGRGFTYLQNALVAAFGPEKAKPILES
                     ISQAMDTAAFSSLKLVDPGILADYLKGEHPQTIALVLAHLDHQKVAQVIKEMPEKLQP
                     EIVFRIANLGMVPAGIIEDVDEVLKKEIQAWAAWRARGSAA"
BASE COUNT          315 a          431 c          459 g          195 t
ORIGIN      
        1 cccggagata cacgtggtgc gctccgagga actcaaaaac acctccgctg ctggcaccgc
       61 cgccacgcag ggggccgccg ggaccccgtc gaacctgccg accggcaggg gcggccccga
      121 ggccgccccc ggggaggccg gcaggaacgc gggcagcatc gtgagaaact acgagatcgg
      181 caggaaccag accgagagca tcttctcgcc gggcgagatc aagcgcctga cggtctccgt
      241 ggtcgtggac gggacgtaca agacggacaa ggacggcaac aagctcttcg tggccaggca
      301 ggcatccgaa ctgaaagaga ttgagaacgc ggtgaagaac gccgtggggt tcaacgcgga
      361 cagggaagac ctcatctctg tctcgtgcat gccgttcgcc caggacgagg tggacctcgg
      421 ctccctatcc aaggagaaga acaaggagct catcatgacc gcggcgaggt acgcgctctt
      481 cttcgtgctc gtgctgctcg tgctcctcct ggtcgtacgt cccctcctca actggctcac
      541 gaagtccgtg agggtggtgg agcacgtgca tgcccgagag cgggaggtgg gctacgaaga
      601 acccgccatg atcgagggtt cggagggggt cccccggata gaggcctcgg ccaagagcga
      661 ggagatgaag cgcgcggtac agggtaaacg caagaccatc gagaacatca cgaagaacga
      721 catgaacacc gccacggcgg tggtgaagac ctggcttcag gagagctcat gacgctgaaa
      781 accaagaaga tggacggccc gcagaaggcc gcgatcttcc tcatggcaat gggtgacgac
      841 ttcacccgcg aggtgatcaa gggcctcaac gagcacgagg tgacgctcct cggcaggcgc
      901 atggccgagc tggagaacgc caccataccg gtggacacgg tcaggaccat catggacgag
      961 ttcgagcagc tctcgaacga ggtcagcggt gtcacgggcc gggggttcac ctacctccag
     1021 aacgccctcg tggcggcgtt cggccccgaa aaggccaagc cgatcctgga atccatatcc
     1081 caggccatgg acaccgccgc gttctcgtcg ctcaagttag tggacccggg catcctggcg
     1141 gactacctca agggcgagca cccccagacc atcgccctcg tgctggccca cctcgaccac
     1201 cagaaggtgg cgcaggtcat caaggagatg cccgaaaagc tccagcccga gatcgtcttc
     1261 cgcatagcga atctcggcat ggtgcccgcc gggatcatcg aggacgtcga cgaggtcctc
     1321 aagaaggaga tccaggcatg ggcagcgtgg agagcaagag gctcggcggc atagagaccg
     1381 tcgccgagat catgaaccag
//
LOCUS       DAOSCR010000683         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_62963_length_1400_cov_3.176208, whole genome shotgun sequence.
ACCESSION   DAOSCR010000683 DAOSCR010000000
VERSION     DAOSCR010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62963_length_1400_cov_3.176208"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLT69_12750"
     CDS             <1..385
                     /locus_tag="PLT69_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOJ15355.1"
                     /translation="FRPPAEAPDEEFDLTLTTGRIYYQFHTGTMSRRIEILEREAPQP
                     VLEINPEDAKRYGIRTNDLVEVESRRGSIRLKAEVTTRVPRKVLFTTFHFAESPVNRL
                     TTSAFDPVSEIPELKGCAVKIRRCS"
     gene            382..>1400
                     /locus_tag="PLT69_12755"
     CDS             382..>1400
                     /locus_tag="PLT69_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ15356.1"
                     /translation="MKLIEKTKIKGVLAEWSKDFNVLCPSLTPNGDCLFDDFDPQTFT
                     LEYRKPSMPQKRSCFPQAEKIFDVREGSFVPAYNHKKTVLFGIRACDHMGLRQSKSFF
                     TRDIVDPFFGCRSEDTVIVVHACSWPQNETCFCTTVHSGPYATADFDIQLFDMGTDFL
                     VEAGSDKGRRMISSSPFRDFDVDDAQERLERVKHMAFNSIPIVEEVAEAMDILKAGGA
                     NETVWEHLGRKCIACGGCVYVCPTCTCFTIHDRVTSKDSGERVRTWDTCLFEGFTKEA
                     SGHNPRPTQALRLKRRHEHKLLHYNPVDIQTALSGCVGCGRCSDSCPVHIGTLEVVKA
                     ITDHI"
BASE COUNT          300 a          445 c          427 g          228 t
ORIGIN      
        1 attccggccc cccgcggaag cgccggacga ggagttcgat ctcaccctga ccacggggcg
       61 catctactac cagttccaca cggggaccat gtcccggcgc atagagatac tcgagcgcga
      121 ggccccgcag ccggtgctcg agatcaaccc ggaagacgcg aagagatacg gcatccgcac
      181 caacgacctc gtggaggtcg agtccaggag agggagcatc cggctgaagg ccgaggtgac
      241 cacgagggtg cccagaaagg tcctcttcac gacgttccac ttcgcggaat cgccggtgaa
      301 caggctcacc acgtccgcct tcgatcccgt aagcgagatt cctgagctca agggttgcgc
      361 ggtgaaaatc aggaggtgct catgaagctc atcgagaaga cgaagatcaa gggcgtcctc
      421 gcggagtggt ccaaggactt caacgtgttg tgccccagcc tgacgccgaa cggcgactgc
      481 ctgttcgacg atttcgaccc gcagaccttc accctggagt acaggaaacc gtcgatgccc
      541 cagaagcgct cgtgcttccc ccaggcggaa aagatcttcg acgtcaggga aggctcgttc
      601 gtccctgcat acaaccacaa gaagacggtc ctcttcggga tcagggcgtg cgaccacatg
      661 gggctcaggc agtcgaagag cttcttcacc agggacatag tagacccctt cttcgggtgt
      721 cggtcggaag acacggtgat cgtcgtgcac gcctgcagct ggccgcagaa cgagacgtgc
      781 ttctgcacga ccgtgcacag cggtccatac gcaacggccg acttcgacat ccagctcttc
      841 gacatgggca ccgacttcct ggtcgaggcg ggatcggaca agggcaggcg gatgatctcc
      901 tcgagcccgt tcagggattt cgacgtggac gacgcccagg aacgcctcga gcgcgtcaag
      961 cacatggcgt tcaactccat accgatcgtg gaggaggtcg ccgaggcgat ggatatcctc
     1021 aaggccggcg gcgcgaacga aaccgtctgg gaacatctcg gcaggaagtg catagcctgc
     1081 ggcgggtgtg tctacgtgtg cccgacctgc acctgcttca ccatccatga ccgcgtcacc
     1141 tccaaggact cgggtgagcg cgtacggaca tgggacacct gcctgttcga gggcttcacc
     1201 aaggaggcct caggtcacaa cccgaggccg acccaggccc tgcgcctcaa gaggaggcac
     1261 gagcacaagc tcctccacta caaccccgtg gacatccaga cggcgctgag cggttgcgtc
     1321 ggctgcggga ggtgttcaga ctcctgcccc gtccacatag ggaccctcga ggtggtcaag
     1381 gccatcaccg accatatcta
//
LOCUS       DAOSCR010000684         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_63020_length_1399_cov_2.957589, whole genome shotgun sequence.
ACCESSION   DAOSCR010000684 DAOSCR010000000
VERSION     DAOSCR010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63020_length_1399_cov_2.957589"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..740
                     /gene="rfbC"
                     /locus_tag="PLT69_12760"
     CDS             198..740
                     /gene="rfbC"
                     /locus_tag="PLT69_12760"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOJ15357.1"
                     /translation="MKISESTLAGAFIIEQDLHEDARGAFMEVFNAPVLERHGLGAAF
                     VQDNCSLSRRAGVIRGLHFQRDPHAQAKLVWVVTGSIFDVIVDLRPGSRTRFKWESFI
                     LTDERPCMLYIPKGFAHGFCTLKDDTRVFYKVDSPYNPGSEAGIRWDDPDLGIPWPTV
                     SPILSEKDARLPLLRELAGS"
     gene            1112..>1399
                     /locus_tag="PLT69_12765"
     CDS             1112..>1399
                     /locus_tag="PLT69_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15358.1"
                     /translation="MTIMCIMGSGRLWWITSVHGHEDGSGCVRRYRRIPFNRLRPGAS
                     YGFDLYTVRGGTMQLLAARGVPLNSERLGLSGTQGVQVFVHAADWEQACRET"
BASE COUNT          210 a          468 c          475 g          246 t
ORIGIN      
        1 gaatgcctct cgggcatgca tggaggtcac ctggcggcgg tggctcacct tcgcgcactt
       61 ggcgggcctg cgcgcgcatc gtccgtggaa tgccgccgac tgggcaggcg agcgcgtgcc
      121 ctccttcttt atggccttca gcgctctttc tttaggtgct tgatagacga cccgcccatg
      181 aggtaatctc gcactcgatg aagatcagcg agtccacgct tgccggcgcg ttcatcatcg
      241 agcaggacct ccacgaggac gcgagggggg ccttcatgga ggtcttcaac gcgcccgttc
      301 tcgagaggca cggcctgggc gccgccttcg tgcaggacaa ctgttcgctc tcgaggcgcg
      361 ccggcgtgat ccggggcctg cacttccagc gcgaccccca cgcacaggcg aaactcgtct
      421 gggtcgtcac gggatccatc ttcgacgtga tcgtggatct gaggccgggc tcacggacgc
      481 ggttcaagtg ggagtcgttc atcctcaccg acgagagacc gtgcatgctc tacatcccga
      541 aaggcttcgc acacgggttc tgcacgctca aggacgacac gagggtcttc tacaaggtgg
      601 actcgccgta caaccctggc tccgaggcgg gtatccgctg ggacgacccc gatctcggca
      661 tcccgtggcc cacggtgtcc cccatcctgt cggaaaagga cgcgcgcctg ccgttgctga
      721 gggagctcgc gggttcgtag ctcgcccggg tcgagcccgt tctcaccggc tggcgcatgg
      781 acttgcggtg agcaccccgc tttggggaag cccacgtcgt ggccctgcag actgaggagc
      841 gcgggcgtca cccggcgcaa ggcgctgctg gaggggcccc tcgccgttgc atccacgggc
      901 ggcccggcgg cgacggtccc cgcccgcgca agcacagggt cggcgcggcc attgcccgtg
      961 ggtgcgccca ggacgccccg ccccggacgg tccctgcgcg tgcgcgccgt cccccatggt
     1021 ctatcaacct cgtggcctcg atgcatccct gccccagccc ttaaggacgt gtcttgccgc
     1081 cgggtgggag cggttcaagg tatccgcatc gatgacgatc atgtgtatca tggggtcggg
     1141 acgcctgtgg tggatcacga gcgtgcacgg gcacgaggat ggctcggggt gcgtgcggcg
     1201 gtaccggagg atccccttca accggctgag accgggcgcc agctacggtt tcgacctcta
     1261 caccgtgagg gggggcacga tgcagctctt ggccgcgcgt ggagtccccc tcaattcgga
     1321 gaggctcggc ctgtcaggca cgcaaggggt tcaggtcttc gtccacgccg cggactggga
     1381 gcaggcctgc cgagagacc
//
LOCUS       DAOSCR010000685         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_63095_length_1398_cov_2.616530, whole genome shotgun sequence.
ACCESSION   DAOSCR010000685 DAOSCR010000000
VERSION     DAOSCR010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63095_length_1398_cov_2.616530"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PLT69_12770"
     CDS             <1..1125
                     /locus_tag="PLT69_12770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ15359.1"
                     /translation="HVLFVMNEMPRVYEKTRLFLPSKDYLNMRLTGERAATFDSVHLF
                     WLTDVRDLARIRYDKDLVRESGIDPSKLPPLVASTDVLGHIAPGVADEMGLRQGVKVV
                     AGSPDHQCACIGSAAVEDYQGHLYVGTSSWVQCLVPFKKTDVLHSIASFPTAVGDRYQ
                     AVNEQDMAGGALGFLAGLVNGFSAMSAGAAGQGISYNALDEIAKGVPPGSRGVVVTPW
                     LNGERTPVDDMHVRAGIHNLSTTTTAEDIVRAFLEGVALNTRWSLKYVERFVGRRLDP
                     LTIVGGGASSDVWCRIFADCLGRGLRRPPEPIRANARGAAFIAAVALKDITFSDIPSL
                     MEYERVFEPGREASRIYDELFDAFVDIYRQNRGLYRALNG"
BASE COUNT          235 a          457 c          475 g          231 t
ORIGIN      
        1 cacgtcctgt tcgtgatgaa cgagatgccc cgtgtctacg agaagacccg cctcttcctc
       61 ccgagcaagg attacctgaa catgaggctc acgggagagc gggcagccac cttcgactcg
      121 gtccacctct tctggctcac cgacgtgagg gatctcgccc gcatccgcta cgacaaggac
      181 ctcgtgcgcg aaagcgggat cgacccttcc aagctgcccc ccctggtggc ctccacggac
      241 gtgctcgggc acatcgcgcc aggggtcgca gacgagatgg gcctgaggca gggggtcaaa
      301 gtggttgccg gctcgccgga ccaccagtgc gcgtgtatcg gctcggcggc ggtggaggat
      361 taccaggggc acctctacgt tggcacctcg tcgtgggtgc agtgcctcgt ccctttcaag
      421 aagacggacg tgttgcattc catcgcgtcc ttccccacgg cggtgggcga caggtaccag
      481 gcagtgaacg agcaggacat ggcgggaggg gccctcgggt tcctcgccgg tctcgtgaac
      541 ggtttcagcg cgatgtctgc cggcgccgca gggcagggga tctcctacaa cgcgctcgac
      601 gagatcgcga aaggcgtccc cccgggcagc cggggcgtcg tcgtgacgcc gtggctcaac
      661 ggtgagcgca cccccgtgga cgacatgcac gtgcgcgcgg gcatacacaa cctcagcacg
      721 acgacgacag ccgaggacat cgtccgggcc ttcctcgagg gcgtggccct gaacacgcgc
      781 tggagcctga agtatgtcga gcgcttcgtg ggccgcaggc tggaccccct caccatcgtg
      841 gggggcggcg catcgtcgga cgtgtggtgc cggatattcg ccgactgtct cggacggggc
      901 ctgaggaggc cgccggagcc catacgcgcc aacgcccgcg gcgccgcctt catcgccgcc
      961 gtggcgctca aggacatcac gttttcggac atcccctcgc tcatggagta cgagagggtc
     1021 ttcgagcccg ggcgagaggc atcccgcatc tacgacgagc tcttcgacgc gttcgtggac
     1081 atctaccggc aaaaccgggg gctgtaccgg gcgctcaacg ggtagcctca ccccggtagc
     1141 cgacgagccg atgcgtcttg gggctgcgta cggggagggt cgcatgtccg gcaaaagagc
     1201 ggctcctcag gcgccaggcg tcgtgcacgc aaggaagagg aatcttcccc gcacccaggg
     1261 gtcatgggcg caccatgtgc gcatcgagaa ggcatgcgct cgagttttcc ccgcacccag
     1321 gggtcaatgg ccgggttcgt gaatccggcg cgcggtgatc ttgagttttc cccgcaccca
     1381 ggggtcagtg gttggcgt
//
LOCUS       DAOSCR010000686         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_63698_length_1389_cov_3.263118, whole genome shotgun sequence.
ACCESSION   DAOSCR010000686 DAOSCR010000000
VERSION     DAOSCR010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63698_length_1389_cov_3.263118"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            688..>1389
                     /locus_tag="PLT69_12775"
     CDS             688..>1389
                     /locus_tag="PLT69_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ15360.1"
                     /translation="MACKSLMVALAVAAALLASWVQPPPLHAANPDEGAPRLIRVAID
                     DNYPPYIFRNDKGQIQGILVDLWRLWEQKTGIRVVLLAMDWSHALDTMKSGGADVIDT
                     IFFTEARSRIYDYSKPYATINVPVFFHRTLSGISGVGSLLGFRIGVKRGDACIDVFKS
                     HGIEKGLVEYDSYEAIVKDAAMGRIRVFCIDEPPAFFFLNKYDILDQFHYTDPLYSGE
                     FHRAVAKGNTRLLAIV"
BASE COUNT          248 a          492 c          417 g          232 t
ORIGIN      
        1 gatccactgt ggagtcttgc cgcaaccgca ccctctatta tacaccatgg ggccgaaggg
       61 tccaggggct ttcacgcgcg gcgtccccgg taccacgccc accccttccc ccctcctcac
      121 gcccccaccg gggacgtccg agcgcacctc accgcagcct cacgaggggg gtcgcggggc
      181 tcatcccgcg gtgttttggc gacttcggta cctctaggca cccacgagac ccgtcttcca
      241 ggtctttttc ctcgcgcacc ccatcgcagg cggcaccgct ggtcaccccg acacgcatcc
      301 cccacccctt gccggtcctc gtcgccggag cataccgccg gagtttcgga cctcgctcgg
      361 ccatcgaacg gcctccctcc cctggtcgtc tcgcttccgg gaaacccggg tgccgtaccg
      421 tggacacgca ccgtcatgag gagaaaaagg cggcgctcca tcccaggagg cgtagccgaa
      481 gggggatctc cccacaggaa gactccaggg gagaaacgag ggggcgttcc ataggccggc
      541 cccggtcccc tccgacatgg cctgcggccc tggcccggcc cacaccttga cctctcgaga
      601 aaccggaata tatttgtcgg tatggggatc atcctgagag ggggggaaga ccgaccgcgt
      661 cgccggtggt ctgcgccgag ggcaggcgtg gcatgcaaga gcctcatggt cgccctcgcc
      721 gtagcggcag ccctgctcgc gagctgggta cagccgccac cgctccacgc ggcgaacccg
      781 gacgagggcg cacccaggct gatcagggtg gccatcgacg acaactaccc gccctacatc
      841 ttcaggaacg acaagggcca gatccagggc atcctcgtcg atttgtggag gctctgggaa
      901 cagaagacgg ggatccgcgt cgtgctgctc gccatggact ggtcgcacgc cctggacacc
      961 atgaagagcg ggggggcgga cgtcatcgac acgatcttct tcaccgaggc acgctcgcgc
     1021 atctacgact actcgaagcc gtacgccacc atcaacgtcc ccgtcttctt ccacaggacc
     1081 ctttcgggca tcagcggcgt gggctccctc ctcgggttca ggataggcgt caagcggggg
     1141 gatgcctgca tagacgtgtt caagtctcac ggcatcgaga agggcctcgt ggaatacgac
     1201 agctacgagg cgatcgtgaa ggacgcggcc atgggcagga tcagggtctt ctgcatcgac
     1261 gagccgcctg ccttcttctt cctgaacaag tacgacatcc tcgaccagtt ccattacacc
     1321 gacccgctct actctggcga gttccaccgc gccgttgcca aggggaacac ccggctgctc
     1381 gcgatcgtg
//
LOCUS       DAOSCR010000687         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64066_length_1384_cov_2.988713, whole genome shotgun sequence.
ACCESSION   DAOSCR010000687 DAOSCR010000000
VERSION     DAOSCR010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64066_length_1384_cov_2.988713"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLT69_12780"
     CDS             <1..379
                     /locus_tag="PLT69_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ15361.1"
                     /translation="GGVDLNEDGKALLKGLVPVFKGLDRKLIIEGHTDTTGSREKNWD
                     ISTQRALSARFELERDGIPPDQIEMVAGYASTRPFIKSNPADPKNRRVSIVVDYKTPR
                     YQPSPGQIMQKPPLPDSPLGLGS"
     gene            762..1283
                     /locus_tag="PLT69_12785"
     CDS             762..1283
                     /locus_tag="PLT69_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOJ15362.1"
                     /translation="MTGWSEEEIANKELMAPCGLYCGVCGVYIATRDDNLKFKTAMGN
                     LYGTKPEETECLGCMQPDPPRKLYGFCTMCTIRECVKSKGFYSCHQCADWPCHQITNF
                     GLATGRKVMLETIPLWRELVARLGDEEGSREWARRVCERYHCPACDSPLFRGAQRCRS
                     CGRKVADELDGSL"
BASE COUNT          278 a          442 c          456 g          208 t
ORIGIN      
        1 aggcggcgtc gatctcaacg aggacgggaa ggcccttctc aagggtctcg tcccggtctt
       61 caagggcctc gaccggaagc tcatcataga ggggcacacc gacacgaccg ggtcacgcga
      121 gaagaactgg gacatctcca cgcaacgcgc actctcggcc aggttcgaac tcgagagaga
      181 cgggataccg ccggaccaga tcgagatggt cgccgggtac gcctcgacaa ggccgttcat
      241 caagagcaac cccgctgacc caaagaacag gcgggtgagc atcgtcgtgg actacaagac
      301 cccccgctac cagccgtcgc cggggcagat catgcagaag cccccgctcc cggactcacc
      361 cctcggcctg gggtcgtgag ttctccggtt gctcgtccgt agacactctc cgacacgtga
      421 cacatcgcca gggaggggct ccggcccagc caagagccgg gaaagggccg gcggccgtgc
      481 ccgtccagtc ccgccgtcga gggaacgtat gacggcgggg tgccgagaca ggcgccatgc
      541 catggttcga cggccggccg catacgagcg cacgcaggag acacaggccg gatgcgcgaa
      601 ggccaatgtc tcgaccttcc catgagcggg cttgtcgcat cttgcccgtg cacccggccc
      661 acagcgcacg gccctttaca caggcgtcct ttttgggtca tcatggtgtc gctggaaaga
      721 cacggccggg acccggccgg ccgacccgaa ggaggcccaa gatgacagga tggtccgagg
      781 aggagatcgc caacaaggaa ctcatggcgc cctgcggcct gtactgcggg gtctgcgggg
      841 tgtacatcgc caccagggac gacaacctca agttcaagac cgccatgggg aatctctacg
      901 gcacgaaacc cgaggagacc gagtgcctgg gctgtatgca gcccgacccg ccgaggaagc
      961 tctatggttt ttgcacgatg tgtaccatca gggagtgcgt caagtcgaag gggttctatt
     1021 cctgccacca gtgcgccgac tggccgtgcc accagatcac gaacttcggg ctcgcgacag
     1081 ggaggaaggt catgctcgag accatccccc tctggaggga gctggtcgcg aggctcggcg
     1141 acgaggaggg aagcagagag tgggcccgca gggtgtgcga gcggtaccac tgccctgcct
     1201 gcgattcccc cctgttcagg ggcgcacagc gctgccgctc gtgcgggagg aaggtggccg
     1261 acgaactcga cgggtcgttg tgagatgggg tggaggctca tgaccttcca gcgacgcttt
     1321 cgtatccatg cccacgaaca gaccgcccat gatcggctgc ctgcggagga acgctcatga
     1381 tcca
//
LOCUS       DAOSCR010000688         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64086_length_1384_cov_2.419112, whole genome shotgun sequence.
ACCESSION   DAOSCR010000688 DAOSCR010000000
VERSION     DAOSCR010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64086_length_1384_cov_2.419112"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="tal"
                     /locus_tag="PLT69_12790"
     CDS             <1..1086
                     /gene="tal"
                     /locus_tag="PLT69_12790"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOJ15363.1"
                     /translation="LHELARVGQSVWFDYIQRSLISSGELSRLIREGVRGITSNPAIF
                     EKAITQSGDYDVEIAVLAREGKDVLEIYESLVMKDIALACDVFMELYAESRGVDGYVS
                     VEVSPHIAYDADSTVREASRIFELLAKPNVMIKVPATKEGLTAIEELTARGVNVNATL
                     IFSVKRYEEVARAHLRGLQRRLDGGLPLDSTASVASFFVSRIDTAVDAELDRMGMKGL
                     QGKAAIASARLAYARFKELFSGGPWDRLASRGARVQRPLWASTGTKNPGYPDTMYVDN
                     LVGAHTVNTLPPATLGAYIDHGRTAATLETGLSDAEKTMEELEAAGIDLASITARLEE
                     EGVKAFQRSFDTLLEGIGRKRDEVTGG"
     gene            1083..>1384
                     /locus_tag="PLT69_12795"
     CDS             1083..>1384
                     /locus_tag="PLT69_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15364.1"
                     /translation="MTAIELVLGPYRTAVESRLSALTRARVVDRIHDLDHTVWKMDPH
                     EITNRLGWLHCPETMRGRIPELERFTAEARGAGYIDAVVLGMGGSSLAPEVFAKTFP"
BASE COUNT          268 a          438 c          460 g          218 t
ORIGIN      
        1 ctacacgaac tcgccagggt cgggcagtcg gtttggttcg actacataca gcggtctctc
       61 atctcgagcg gtgagctctc caggctcata cgcgaggggg tgaggggcat aacgagcaac
      121 cccgccatct tcgagaaggc catcacccag agcggcgact acgacgtgga gatcgccgtg
      181 ctcgccaggg aaggcaagga cgtcctcgag atctacgagt cgctcgtgat gaaggacatc
      241 gcactggcct gcgacgtctt catggagctc tacgcggagt cgaggggcgt cgacggctac
      301 gtgagcgtcg aggtctcccc ccacatcgcc tacgacgccg actccacggt gagggaggca
      361 tcccgtatct tcgagctcct ggccaagccc aacgtgatga taaaggtccc tgccacgaag
      421 gagggcctca ccgccatcga agagctcact gctcgagggg tgaacgtgaa cgcgaccctc
      481 atcttctcgg tgaaacggta cgaggaggtg gcgcgggccc acctcagggg gctccagagg
      541 aggctcgatg gcgggctgcc cctcgattcc acggcgtcgg tcgcctcgtt cttcgtgagc
      601 aggatcgaca ccgccgtgga cgccgagctc gaccgcatgg gcatgaaagg cctccaggga
      661 aaggccgcca tagcgagcgc ccgcctggcc tatgcgcgtt tcaaggaact gttctcgggt
      721 ggtccgtggg atcgcctcgc atcgcgtggc gccagggtcc agaggccgct gtgggcgagc
      781 accggaacca agaacccggg gtacccggac accatgtacg tggacaacct ggtgggcgcc
      841 cacaccgtga acaccctccc gcccgcgacg ctcggcgcat atatcgatca cggccggacg
      901 gcggcgaccc tcgagaccgg ccttagcgac gccgagaaga cgatggaaga gctcgaggcc
      961 gccgggatcg atctcgcatc cataacggcc aggctcgagg aagagggtgt aaaggctttc
     1021 cagcgatcgt tcgatacgct cctggaaggc atcgggagaa aacgtgacga ggtgacgggg
     1081 ggatgacggc gatcgaactt gtcctggggc cgtacaggac cgctgttgaa tccaggctct
     1141 cggccctgac cagggcccgc gtggtggacc gcatccacga tctcgaccac acggtgtgga
     1201 agatggaccc tcacgagatc acgaaccgcc tgggctggct tcattgcccg gagaccatgc
     1261 gcggcaggat acccgagctc gagcgattca ccgccgaggc gaggggcgcg ggctacatag
     1321 acgccgtcgt gctcggcatg ggcgggtcgt cccttgcccc cgaggtcttc gcgaagacct
     1381 tccc
//
LOCUS       DAOSCR010000689         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64326_length_1380_cov_3.578113, whole genome shotgun sequence.
ACCESSION   DAOSCR010000689 DAOSCR010000000
VERSION     DAOSCR010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64326_length_1380_cov_3.578113"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLT69_12800"
     CDS             complement(<1..354)
                     /locus_tag="PLT69_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ15365.1"
                     /translation="MAGIAIGVMALIVVLSVMNGFEKDIKAKLLGTQSHIVISHYEGG
                     VRDPRGVEKALSTIDQVEAATPFILSQGLVTSAGGTSGAVIRGIDPQSAYKVIRLKDI
                     LTKGTADLDDSGVLIG"
     gene            complement(435..>1380)
                     /gene="lysS"
                     /locus_tag="PLT69_12805"
     CDS             complement(435..>1380)
                     /gene="lysS"
                     /locus_tag="PLT69_12805"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOJ15366.1"
                     /translation="RIISAIRAYMDERGFLEVETPMMQAIPGGATAKPFRTHHNALDM
                     DLYMRIAPELYLKRLLVGGFEKVYEINRNFRNEGISTRHNPEFTMMEFYHAYADHEDL
                     MRFTEDMISHVAREVLGTTVITYQGQEIDLGAPWARMTLKEAACAVGGVAPEVLEDRA
                     KALSFCSKAGIEVSGGESLGDLQLAIFEKTAEDKLVGPVFITSYPREVSPLSRPNGND
                     PSVVDRFELYIAGREIANAFSELNDPVDQRSRFEEQVRRKEGPGEVDEDYIRALEYGM
                     PPAAGEGIGIDRLVMLLTDSPSIRDVILFPLLRKGDLP"
BASE COUNT          216 a          439 c          445 g          280 t
ORIGIN      
        1 cccgatgagc acccctgagt cgtcgaggtc tgcggtgccc ttggtgagga tatccttgag
       61 cctgatgacc ttgtaggcgc tttgcgggtc gatccccctg atcaccgccc ccgaggtgcc
      121 gcccgcggag gtaaccagcc cctgggagag gatgaagggt gtcgcggcct cgacctggtc
      181 tatggtggac agcgccttct ccactccccg ggggtcgcgc acgccgccct cgtagtggga
      241 gatgacgatg tgcgactggg tgccgagcag cttggccttg atgtccttct cgaagccgtt
      301 catgaccgag aggaccacga tgagcgccat gaccccgatg gcgatgcccg ccacggagag
      361 gccggaggtg aagcttatga aacgctggcc tttcttggct ctcaggtact tctttgcgac
      421 gaggaactcg aatctcaggg caggtctcct tttcgcagca ggggaaagag gatcacgtcc
      481 ctgatggagg gactgtcggt caggagcatg acgagcctgt ctatgcctat gccctcaccg
      541 gcggccgggg gcatgccgta ctcgagggcg cggatgtaat cctcgtccac ctcgccgggg
      601 ccttccttgc ggcgtacctg ttcctcgaac ctcgagcgct ggtcgaccgg gtcgttgagc
      661 tcgctgaagg cgttcgcgat ctcccgtccc gcaatgtaga gctcgaagcg gtccaccacg
      721 gaggggtcgt tcccgttggg cctcgacagg ggggatacct ccctcgggta cgaggtgatg
      781 aacacgggcc ctaccagctt gtcttccgct gtcttctcga agatggcgag ctggaggtct
      841 cccaggcttt caccccccga cacctcgatg cccgccttcg agcagaagga caaggccttc
      901 gccctgtctt ccagcacctc gggggccacg ccgcctacgg cgcaggccgc ctccttgagg
      961 gtcatgcgcg cccacggcgc gcccaggtcg atctcttgcc cctggtaggt gatcaccgtc
     1021 gtccccagca cctctctcgc cacgtgcgag atcatgtcct cggtgaagcg catgaggtcc
     1081 tcgtggtcgg cgtaggcatg gtagaactcc atcatggtga actcggggtt gtggcgggtg
     1141 gagatcccct cgttcctgaa gttgcggttg atctcgtaga ccttctcgaa cccgcccacc
     1201 aggagccgct tgaggtagag ctcgggcgct atcctcatgt agaggtccat gtcgagggcg
     1261 ttgtggtggg tccggaaggg cttcgccgtc gcgccgcccg ggatggcctg catcatgggg
     1321 gtctccacct cgaggaagcc cctctcgtcc atgtacgccc tgatggcaga gatgatcctg
//
LOCUS       DAOSCR010000690         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64447_length_1379_cov_2.092900, whole genome shotgun sequence.
ACCESSION   DAOSCR010000690 DAOSCR010000000
VERSION     DAOSCR010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64447_length_1379_cov_2.092900"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(707..>1035)
                     /locus_tag="PLT69_12810"
     CDS             complement(707..>1035)
                     /locus_tag="PLT69_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOJ15367.1"
                     /translation="AKPRALEMVLADLRDRVNTEFDPKVVVHVPQVVRAYLERVAAKG
                     GTAEVELLPHELKPGMVVSRNVTSGTGVLLLSQGTRLTEKNIETLCRMHRIDPSREGV
                     FVWMNR"
     assembly_gap    1036..1045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1046..>1379)
                     /locus_tag="PLT69_12815"
     CDS             complement(<1046..>1379)
                     /locus_tag="PLT69_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ15368.1"
                     /translation="IARAMKLPQADVETIMVASLLHDIGLIGVPDVMLLKAESEMNAD
                     EIAEFRLHPIRGQAAVDQIEDLRPVSALIRHHHERYDGKGYPDGVKGEEIPLGARIMA
                     VADHIDSTM"
BASE COUNT          233 a          448 c          409 g          279 t
ORIGIN      
        1 cgctatgcgc ttcctggcct ttcccactgg atgcacccca tgaacgtcac cccaggcatc
       61 cttgtgccca ggctccggtc aaatacgata ccagatcctt acctgataac acacctgaag
      121 atgttcgtac cacagaaata aagaaagatg acaacctgtt cgcctggttg agaaaaatga
      181 tattccctga tgacacgatc gtactcccgg ctcatcacca gcaacccttc gcgtgactgg
      241 ccgcctcttc cgccatgatg caccaccgat tgttttcgat atcccgttgc aggatgcatc
      301 cgccaggtta ttcaggccgg gggccttctc actagccggc atcttcgaga agacgccgac
      361 cctatgctgg tgcgatcaca tggcatggtc gtctcgtttg ccgtcaagag aagccagcca
      421 tgccctttca tgcgcagagc cctcagcgat gaagctttgc ccagggctca cccctggggg
      481 cacccggcgc ccgccgaggt ccttgtgaca ggcgttgcga cgggtgcgcc ctgtcgcaga
      541 ggtatgacct ccctgcggat gtcttgtgca cctcgttgcc cgagggtcac cctcgaggga
      601 gggtgttgtc gaaggagccg cgtgggaaga gctggaaaga gccgggccag cacaccggct
      661 gttcgcggcc gcttgaaggg tggacgcggc cgaggcgcct gctggctcac cggttcatcc
      721 agacgaagac gccctctctc gagggatcta tccggtgcat gcggcaaagc gtctcgatgt
      781 tcttctcggt gagcctcgtc ccctggctca agaggagcac gcccgtgccg ctcgtgacgt
      841 tcctggacac caccatgccg ggcttgagct cgtgcggcaa cagctccacc tccgccgtgc
      901 cgcccttggc agccacgcgc tcgaggtacg cccggaccac ctggggcacg tgcacgacga
      961 ccttggggtc gaactccgtg ttgaccctgt cccggaggtc tgcgaggacc atctcgagcg
     1021 ccctcggctt cgccgnnnnn nnnnncatgg tagagtcgat gtggtcggca acggccatga
     1081 tccgtgcgcc gagcgggatc tcctcgccct tcaccccgtc cgggtagccc ttcccgtcgt
     1141 atcgctcgtg gtggtggcgg atcagggctg agacgggcct gaggtcctct atctggtcga
     1201 cggcggcctg tccccttatg gggtggagcc tgaactcggc tatctcatcg gcgttcatct
     1261 cgctttctgc cttgaggagc atgacatctg gcaccccgat gagcccgatg tcgtgcagga
     1321 gagaggccac catgatggtc tccacgtccg cctgggggag cttcatggcc ctggcgatg
//
LOCUS       DAOSCR010000691         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64625_length_1376_cov_3.707040, whole genome shotgun sequence.
ACCESSION   DAOSCR010000691 DAOSCR010000000
VERSION     DAOSCR010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64625_length_1376_cov_3.707040"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLT69_12820"
     CDS             <1..834
                     /locus_tag="PLT69_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15369.1"
                     /translation="DGHPRQDRPCGHGRTLPGGIDFSPADTPIEAYTKLNETVPDTGR
                     IIADRDEIVRIFENVQKLSRGFGDMLMIHRSKAIPAEFFYLRPEQDNMVLRIVRISDL
                     RLPFQPAVDMVYPFYLFRGTDVMLFTARVLDMVKNILEVSWPDTLRHMSRRTVLRYLV
                     TGESPLTATLVHPMNSQRVKVLVWDISIEGMGVEILSDSPPFIEGMNLPSVVIHLGGT
                     LLKATGVVRSVRADAVLEKTQVGIEFTGGAPQYQDKILSYILEMNLPAETLIPKVNHA
                     R"
     gene            complement(822..>1376)
                     /locus_tag="PLT69_12825"
     CDS             complement(822..>1376)
                     /locus_tag="PLT69_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ15370.1"
                     /translation="YAATKKANELMAHTYAALYGLPCTGLRFFTVYGPWGRPDMALFT
                     FTKAILEGRPIDVYNFGDMKRDFTYIDDIVEGLVRVMHLVPSPNPAWNAAEADPGTSF
                     APYRIYNIGGSRPVDLMRFIEVLEDALGRKAEKNLLPLQKGDVRQTWADADDLERAVG
                     FRPGVSVEEGVARFVSWYLGYYRA"
BASE COUNT          257 a          449 c          420 g          250 t
ORIGIN      
        1 gacggacatc cacggcagga tcgtccatgt gggcacgggc gtaccctccc gggtggcata
       61 gacttctcgc ccgccgacac gcccatcgag gcctacacga agctcaacga gaccgtcccc
      121 gacacgggga ggatcatcgc cgacagggac gagatcgtgc gtatcttcga aaacgtgcag
      181 aagctttcga ggggcttcgg cgacatgctc atgatccacc gttccaaggc catccccgcg
      241 gagttctttt acctgaggcc ggaacaggac aacatggtgc tcaggatcgt gcgcatctcg
      301 gacctcaggc tcccgttcca gcctgcagtg gacatggtgt accccttcta cctcttcagg
      361 gggacggacg tgatgctctt cacggcccgg gtgctggaca tggtgaagaa catcctcgag
      421 gtctcctggc cggataccct gcgccacatg agcaggagaa ccgtcctgcg ctacctggtg
      481 acaggcgaat cgcccttgac cgcgactctc gttcacccca tgaactcgca gagggtcaag
      541 gtcctcgtct gggacatcag catcgagggc atgggcgtcg agatcctgag cgatagcccc
      601 cccttcatcg agggcatgaa cctcccgtcg gtcgtgatcc acctgggcgg gacgctgctg
      661 aaggccacgg gcgtggtacg ctcggtgagg gcggatgccg tcttggaaaa gacccaggtg
      721 ggcatcgagt tcacgggtgg cgcgccacag taccaggaca agatcctctc gtacatactc
      781 gagatgaacc ttcccgcgga gacgctcatc ccgaaggtca atcacgcccg gtaatagccc
      841 aggtaccagc tcacgaacct cgccacgcct tcctcgacgc tcacgccggg cctgaacccg
      901 acggcacgtt ccaggtcgtc cgcgtcggcc cacgtctgcc tgacgtcgcc cttctgcagc
      961 ggcagcaggt tcttctcagc cttcctcccc agcgcatcct cgagcacctc gatgaacctc
     1021 atgaggtcca ccggcctcga gccgccgatg ttgtagatgc ggtacggcgc gaaactggtc
     1081 ccgggatcgg cctcggcggc gttccacgca gggttcgggg aaggcacgag gtgcatgacg
     1141 cgcacgagcc cctccacgat gtcgtcgatg tacgtgaagt cgcgcttcat gtcaccgaag
     1201 ttgtagacgt cgatgggcct gccttcgagt atggccttgg tgaaggtgaa cagcgccatg
     1261 tctggcctgc cccaaggccc gtagacggtg aagaacctca gcccggtgca cggcaggccg
     1321 tacagcgcgg cgtacgtgtg ggccatgagc tcgttggcct tcttggtggc cgcata
//
LOCUS       DAOSCR010000692         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64717_length_1375_cov_2.589394, whole genome shotgun sequence.
ACCESSION   DAOSCR010000692 DAOSCR010000000
VERSION     DAOSCR010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64717_length_1375_cov_2.589394"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1375)
                     /gene="topA"
                     /locus_tag="PLT69_12830"
     CDS             complement(<1..>1375)
                     /gene="topA"
                     /locus_tag="PLT69_12830"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOJ15371.1"
                     /translation="VCMRQEEIESFVPREYWTVEVFLRTPAQATFKAKVVDPKEIPDE
                     QAARKVSEAIERAAAIRVESVTRQVRSKHPLPPFITSTLQQAASSRLRFSASKTMRIA
                     QQLYEGLPIGGEEITGLITYMRTDSPVVSEEALRAVRDYIPETYGEAYLPEKPRRYKA
                     KKTAQEAHEAIRPTDVRLTPETLGPHLNKDQLALYDLIWKRFVASQMSSASFDQTTVD
                     ISAGDIGLRATGSVMTFDGFLRLYDTNGEDEQDTLPDSLAEGDILSCERIEPIQHFTQ
                     PPPRYTEASLIKELEDKGIGRPSTYAPTVSTLVERGYVSMEGRTLVPTELGRDVNSLL
                     VKHYPNILDIGFTARMEDSLDLIEEGRSGYLDVMGDFYRTYSAEHEAASRNMENLRAE
                     ARPAGIACPACGRDMVVKLGKNGYFLGCSGYPECTNTMEFTRDASGRIVPAPKEETAH
                     TDQTCEKC"
BASE COUNT          187 a          435 c          461 g          292 t
ORIGIN      
        1 gcacttttcg cacgtctggt cggtgtgggc cgtctcttcc ttcggggcgg gcacgatcct
       61 gcccgatgcg tccctcgtga actccatggt gttcgtgcac tcggggtacc ccgagcagcc
      121 gaggaagtag ccgttcttgc ccagcttgac caccatgtcc ctgccgcacg ccgggcacgc
      181 tatgcctgca ggcctcgcct cggcccgcag gttctccatg ttcctgctcg cggcctcgtg
      241 ctcagcggag taggtgcggt agaagtcccc catgacatcg aggtagccgg acctgccctc
      301 ctcgatgaga tcgagggaat cctccatcct cgcggtgaac ccgatgtcga ggatgttcgg
      361 gtagtgcttc acgaggagcg agttcacgtc cctgccgagt tccgtgggga ccagggtgcg
      421 gccctccatg ctcacgtacc ccctctccac cagcgtggag acggtcggcg cgtacgtcga
      481 aggcctgccg atgcccttgt cctccagctc cttgatgagg ctcgcctcgg tgtacctggg
      541 cggcggctgg gtgaagtgct ggatcggctc gatgcgttcg cacgacagga tgtcgccctc
      601 ggcgagggag tcgggcagcg tgtcctgttc gtcctcgccg ttggtgtcgt acagcctcag
      661 gaacccgtcg aaggtcatga ccgatcccgt ggcgcgcagg ccgatgtcgc cggcgctgat
      721 gtccacggtg gtctggtcga atgacgcgct cgacatctgg gaggcgacga acctcttcca
      781 tatgaggtcg tagagcgcga gttggtcttt gttgaggtga gggcccagcg tctcgggggt
      841 gagcctcacg tccgtgggcc tgatcgcctc gtgggcctcc tgggcggtct tctttgcctt
      901 gtacctgcgg ggtttttcgg gaaggtacgc ctcgccgtag gtctcgggga tgtagtccct
      961 gaccgcccgc aacgcctctt cggagacgac cggcgagtcc gtccgcatgt aggtgatgag
     1021 ccccgtgatc tcctcgccgc cgatgggaag gccctcgtag agttgctggg ctatgcgcat
     1081 ggtcttgctg gcggagaacc tgagcctgct ggatgccgcc tgctgcaggg tcgaggtgat
     1141 gaagggcggc agggggtgtt tcgacctgac ctgcctggtg accgactcga cccttatggc
     1201 cgcggcccgc tcgatggcct ccgagacctt gcgcgcggcc tgttcgtcgg ggatctcctt
     1261 ggggtcgacg accttggcct tgaaggtcgc ctgcgcaggc gtcctgagga agacctccac
     1321 ggtccagtat tccctgggca cgaaggactc gatctcttcc tggcgcatgc acacg
//
LOCUS       DAOSCR010000693         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_64718_length_1375_cov_2.540152, whole genome shotgun sequence.
ACCESSION   DAOSCR010000693 DAOSCR010000000
VERSION     DAOSCR010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64718_length_1375_cov_2.540152"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /gene="amrA"
                     /locus_tag="PLT69_12835"
     CDS             <1..429
                     /gene="amrA"
                     /locus_tag="PLT69_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOJ15372.1"
                     /translation="KGRLRGCIGTLEARESILEGIRRNAINAAFKDPRFPPLGKDELA
                     RVRIEVSVLTEPKPLPYKDPDDLVRKLRPGVDGVIIRKGWATATFLPQVWEQLEDPRE
                     FLSHLCLKAGLDAQAWERGGLEVETYQVQCLEEAEAHDNE"
     gene            complement(1189..>1375)
                     /locus_tag="PLT69_12840"
     CDS             complement(1189..>1375)
                     /locus_tag="PLT69_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15373.1"
                     /translation="KGSPVPAVLPQECIEFDRTGQNKGAVHVVVQHLAVDGAIERVTV
                     WPPSAARRGSAPVFPVP"
BASE COUNT          215 a          425 c          492 g          243 t
ORIGIN      
        1 aagggccgcc tcagggggtg catcgggacc ctcgaggcgc gcgagtcgat tctcgagggc
       61 atacgacgca acgcgatcaa tgcggcgttc aaggacccca ggttcccgcc cctgggaaag
      121 gacgagctgg ccagggtccg catagaggtg agcgtcctca ccgagcccaa gcctcttccc
      181 tacaaggacc cggacgatct cgtccggaag ctcagaccgg gtgtcgacgg ggtcatcata
      241 cggaaggggt gggccacggc gaccttcctc ccccaggtct gggaacagct ggaagacccg
      301 cgggagttcc tctcacacct gtgcctcaag gccgggcttg acgcccaggc ctgggagagg
      361 ggcggtctcg aggtggagac ataccaggtc caatgcctcg aggaggcaga ggcccacgat
      421 aatgaataga ccctgccgcc tgcctgctta ggtgcagagg tcccggacga tgcatggacg
      481 ctcttttttg tgagcgccga ggtcaaccgg cctggattct cgccccgata accggttgag
      541 cccactcttt caggctggtg ggtggaggcg tggatcacgg gccggagacc tgagcgcatg
      601 gggtcatccc tccctggagg gtgtgcccgg tttcccattc accatgcggc ccggtgatga
      661 cccgggtcgt gcccgtgtca ttcccctctg gagggcgtgc ccgggttgcc atgcgggaaa
      721 agcttccccc tgatgccgcc tcgtcctgca ggacctctgc cggagacggc ccagttgtcg
      781 cacccggccg ctcgtggatg gggggtgcgg gccggatgtc agggtttcgc ccggcgggca
      841 aggggcaagg cttcctcacg cgtgtagcgg gcgttcgcgt gggacagcgc ttaccgcgac
      901 tatcgccaat gactcctccc gcgtggggcg ggcgttgtgc ggttggcgct tgacggcgcg
      961 gaatgcaccc ggaagaaaag ctgttgtggg ggcgtcgagc tgttcgaaca cctgtgcacc
     1021 agaggggttc agggcttgcc gatgtcgtga ttgcccggtc ggagaggagc tgttcccgcc
     1081 gcgtatcgct tcccctctgg ggatccagga ggcgattccc gagggcaggc cccggtcaga
     1141 cgaggggctt gccgcccctt gggtgatgtg ggcggggatg ggccatgttc agggtacggg
     1201 aaagaccggg gcgcttcctc gtctggccgc cgaaggcggc cagacggtga ccctctcgat
     1261 ggcgccgtcg accgcaaggt gctggaccac gacgtggacg gcgcccttgt tctggccggt
     1321 cctgtcgaac tcgatgcact cctggggcag caccgcgggc acgggcgatc ctttg
//
LOCUS       DAOSCR010000694         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_65432_length_1365_cov_2.173282, whole genome shotgun sequence.
ACCESSION   DAOSCR010000694 DAOSCR010000000
VERSION     DAOSCR010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65432_length_1365_cov_2.173282"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1097
                     /gene="mraY"
                     /locus_tag="PLT69_12845"
     CDS             18..1097
                     /gene="mraY"
                     /locus_tag="PLT69_12845"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ15374.1"
                     /translation="MLYLLLYPLHTTFTAFNVFKYITFRTIYATITALLITLLLGPWF
                     MEQMTKLSLRERINGHTPKTHKEKSGTPTMGGILIASAIVVSTLLWVNPSSIYVWLGL
                     AVLVSYALLGFIDDYIKTVKGNMRGLPGKLRLAIEFAVAAAVGVALVSTAGFDDTIAI
                     PFFKRVRLDLGPLYVPFVMFVIVGTANAVNLTDGLDGLAIGPCTIAAATYIAFAYVTG
                     NFKAATYLQVPFIPGVGELSVFLGALVGAGMGFLWYNTYPAQMFMGDVGSLGLGGILG
                     AVAVMTKQEMLMLIVGGVFFLEVVSVILQVGFFKVTNGKRIFRMAPIHHHFELKGWPE
                     PKIIVRFWIISIFLAMVAISTLKLR"
     gene            1103..1237
                     /locus_tag="PLT69_12850"
     CDS             1103..1237
                     /locus_tag="PLT69_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15375.1"
                     /translation="MQNSAEAIDYLKALLLIYQDQADPLVSTIIAQISQRLEGLKSCA
                     "
BASE COUNT          236 a          459 c          419 g          251 t
ORIGIN      
        1 caagcaggtg gcctgacatg ctctacctcc tcctctaccc cctccacacg acgttcacgg
       61 cgttcaacgt cttcaagtac atcacgttca ggacgatcta cgccaccatc acggccctcc
      121 tgatcaccct gctgctcggg ccctggttca tggagcagat gaccaagctg agcctcaggg
      181 aacggatcaa cggccacacg ccgaagaccc acaaggaaaa gtcggggacg ccgaccatgg
      241 gcggcattct catagcgtcg gccatcgtcg tctccacgct cctgtgggtg aacccatcca
      301 gcatatacgt gtggctcggg ctcgccgtgc tcgtgagcta cgcgctcctc ggcttcatcg
      361 acgactacat caagaccgtc aaggggaaca tgcgcggcct gcccggcaag ctgcgcctcg
      421 ccatcgagtt cgcggtggcc gccgcggtgg gggtggcgct cgtctccacc gccggcttcg
      481 acgacaccat cgcgatcccg ttcttcaagc gcgtgcgcct cgacctcggg ccgctgtatg
      541 tcccgttcgt catgttcgtc atcgtgggca ccgccaacgc cgtgaacctg accgacggcc
      601 tggacgggct cgccataggc ccctgcacca tcgcggccgc gacgtacatc gcgttcgcct
      661 acgtcacggg caacttcaag gcggccacct acctccaggt accgttcatc ccgggggtgg
      721 gcgagctgag cgtgttcctg ggggccctgg tcggcgccgg catgggcttc ctctggtaca
      781 acacctaccc ggcgcagatg ttcatgggcg acgtggggtc gctcggcctc ggggggatcc
      841 tcggcgccgt ggcggtcatg accaagcagg agatgctcat gctcatcgtg ggcggggtct
      901 tcttcctgga ggtggtctcg gtgatcctcc aggtggggtt cttcaaggtc accaacggca
      961 agaggatctt ccgcatggcc ccgatccacc accacttcga gctgaagggc tggcccgagc
     1021 cgaagatcat cgtgcggttc tggatcatat ccatattcct ggcaatggtg gccatctcca
     1081 ccctgaaact gaggtgaaga ccatgcagaa ctccgccgaa gccatcgatt acctgaaggc
     1141 gctcctgctc atctaccagg accaggccga ccccctggta tcaaccatca tcgcccagat
     1201 ctcgcagagg ctggaggggc tgaagtcatg cgcgtagtgg tgtgggggac gggcgtcacc
     1261 gggctcgaga cggcccgcgt gatgagagcc agggggcacg aggtgatcct cgtggacgag
     1321 tcgcctcccc cgcgggatgt cgggatggag gtgcggcccc ccacc
//
LOCUS       DAOSCR010000695         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_65883_length_1358_cov_2.686876, whole genome shotgun sequence.
ACCESSION   DAOSCR010000695 DAOSCR010000000
VERSION     DAOSCR010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65883_length_1358_cov_2.686876"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..968
                     /locus_tag="PLT69_12855"
     CDS             12..968
                     /locus_tag="PLT69_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ15376.1"
                     /translation="MPIAVFTTHFETDGRIVKAVDDISYCLDTGETLGVVGESGCGKT
                     VSALSLLRLIKDPPGRIVQGEIMFEGKDLLALSEEEIRAVRGNRIAMIFQEPMTSLNP
                     VHTIGFQVMEPLMIHKGLSRSDAMRECIELLKTVQIPDADIRARAYPHQFSGGMRQRV
                     MIAMGLACAPRLIIADEPTTALDVTIQAELLELMKRLTVVKGTALIIITHNLGVVARY
                     ADKVMVMYAGRIVEKADTRDLFRAPGHPYTRALLESVPRLDQDLKVKLRPIEGQPPDL
                     AMLPSGCAFHPRCPQCMDRCRHEAPALKNVAERHEVACWIHD"
     gene            961..>1358
                     /locus_tag="PLT69_12860"
     CDS             961..>1358
                     /locus_tag="PLT69_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ15377.1"
                     /translation="MTKKTGHPMIRVRDLKKHFPVYRGVVFRRAVGDIRAVDGVSFVI
                     GEGETLGLVGESGCGKTTTGRTVLQLERPTSGEVLFMGRDITAIDKYERRRLARHMQI
                     IYQDPFSSLNPRMTVRDIVGEPLLIHGMTR"
BASE COUNT          265 a          432 c          438 g          223 t
ORIGIN      
        1 cgcaactcgg cctgcccatc gccgtcttca ccacgcactt cgagaccgac ggccgcatcg
       61 tgaaggccgt ggacgacatc tcctactgtc tcgatacggg cgagaccctg ggtgttgtcg
      121 gtgagagcgg ctgcggcaag acggtgagcg cgctctccct gctgcggctc atcaaggacc
      181 cgccgggcag gatcgtgcag ggggagatta tgttcgaagg caaggacctc ctggccctga
      241 gcgaagagga gatccgcgcg gtgcggggca accggatcgc catgatcttc caggaaccca
      301 tgacctcgct caatccggtg cacaccatag gcttccaggt catggaaccc ctgatgatcc
      361 acaagggcct ctcccgcagc gatgcgatga gggagtgcat cgagctcctg aagacagtgc
      421 agatccctga cgccgacatc agggctcggg cctaccccca ccagttcagc gggggcatga
      481 ggcagcgcgt catgatcgcc atgggcctcg cctgtgcgcc gaggctgatc atcgcggacg
      541 aacccaccac ggccctggat gtgaccatcc aggcggagct cctggagctc atgaaacgcc
      601 ttaccgtggt caaaggcacg gcgctcatca tcatcaccca caacctggga gtggtggcgc
      661 gctatgcgga caaggtcatg gtcatgtatg cgggaaggat tgtcgagaag gcggacacca
      721 gggacctgtt ccgtgcgccg gggcacccct atacgagggc cctcctggag tcggtccccc
      781 ggctggacca ggatctcaag gtcaagctca ggcccatcga gggccagccc ccggacctcg
      841 ccatgctgcc ctcgggatgt gcgttccatc cccgttgccc ccaatgcatg gaccggtgca
      901 ggcatgaggc accagcgctg aagaacgttg cagaacgaca cgaggtggcg tgctggatcc
      961 atgactaaga agacgggcca tcccatgatc agggtcagag acctgaagaa gcacttcccc
     1021 gtgtaccggg gcgtggtgtt caggcgcgct gtcggcgaca tcagggctgt tgacggcgtg
     1081 agtttcgtca tcggtgaggg cgagacgctg gggctcgtgg gcgagagcgg gtgcgggaag
     1141 accaccaccg gcaggaccgt gctccagctc gagcggccca cctccggcga ggtactcttc
     1201 atgggccgcg acatcaccgc catcgacaag tacgagcgga ggcgacttgc aaggcacatg
     1261 cagatcatct accaggaccc gttctcctcg ctgaatcccc ggatgaccgt ccgtgacatc
     1321 gtgggcgagc cgctcctgat ccatgggatg acccgcag
//
LOCUS       DAOSCR010000696         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_65902_length_1358_cov_2.257866, whole genome shotgun sequence.
ACCESSION   DAOSCR010000696 DAOSCR010000000
VERSION     DAOSCR010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65902_length_1358_cov_2.257866"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLT69_12865"
     CDS             <1..256
                     /locus_tag="PLT69_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ15378.1"
                     /translation="APVLVSATLGIASAVLTESALSFLGIGVQPPTPSWGNMLTQGQA
                     VLGIAWWLSFFPGMAILLTVLGYNLLGEGIRDAVDPRLRQ"
     gene            complement(369..1022)
                     /locus_tag="PLT69_12870"
     CDS             complement(369..1022)
                     /locus_tag="PLT69_12870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ15379.1"
                     /translation="MKDGYRFVGFQYELTGALYSLGRIPRCKTAMLKHLKPGDRVLVA
                     GVGHGTEAVEASRLGAHVTAVDISETMLKHFRRRIERDRPPVPIDVVHDDIFNVREEA
                     SYDMVIANFFLNVFSKTRVKEVADHLGTLVRPGGRFVVGEFVLPEPGWMGVIQRINWY
                     IAISFYSLTTEAVFHPVWDYPALVRGAGCEIEEIEYFTVCGVRLYWSILGRKKAEAA"
BASE COUNT          208 a          496 c          401 g          253 t
ORIGIN      
        1 ggcgcctgtc ctcgtgtccg cgaccctggg catcgcgtcc gcggtgctca ccgagagcgc
       61 cctgtccttc ctcggcatcg gggtgcaacc cccgacaccg agctggggca acatgctcac
      121 ccagggccag gcggtgctcg gcatcgcctg gtggctgtcg ttcttcccgg gcatggccat
      181 cctcctgacc gtgctcgggt acaacctcct gggagagggg atcagggacg cggtggaccc
      241 gaggctcagg caatagacga gccctggcgc cgcttcgcgg agtttgaccg aggcctcagg
      301 gccgccgggg ctcgcccgcc gggctcacca tctccagggg tctccggcca cgcagagggc
      361 cctcatcttc aggccgcctc ggccttcttc ctcccgagga tcgaccagta gagcctcacc
      421 ccgcagactg tgaagtattc tatctcctcg atctcgcatc ccgcgcccct gaccagggca
      481 gggtagtccc acacggggtg gaagaccgcc tcggtggtca ggctgtagaa agagatcgcg
      541 atgtaccagt tgatcctctg gatgaccccc atccagccgg gctcgggcag cacgaactcc
      601 ccgaccacga agcgcccccc cggcctcacg agggtgccca ggtggtccgc cacctccttc
      661 acccgcgtct tggagaagac attgaggaag aagttcgcta tcaccatgtc gtaggaggcc
      721 tcttccctca cgttgaagat gtcgtcgtgg accacgtcga tcggcaccgg gggcctgtcc
      781 cgctcgatgc gcctcctgaa gtgcttgagc atggtctcgg agatgtccac ggccgtgacg
      841 tgcgcaccga gcctgctcgc ctccacggcc tcggtcccgt ggccgacccc tgcgacgagg
      901 accctgtccc cgggcttgag gtgcttgagc atggcggtct tgcacctggg gatgcgtccc
      961 aggctgtaga gagcccctgt caactcgtat tgaaacccca cgaatcggta accgtctttc
     1021 atcctcacaa cctccttcac ggcaggaacc ttgtgcaggg ttcggcctcg gccggttccg
     1081 catccactcc caaggctggc acaaagaggc gcgcctctcg gccttcactt gtaccatgcc
     1141 ggctcgatca ttggctaggg gcaacatcca ccgcgggcat gtgcgggcga agggcttcgg
     1201 acctcgccgt gcgcccttct cgcgctcatc ccctcttctc cggggcacac cttcgcaggc
     1261 ccctttctcg acgcgaggac ccaccccttt cgcccttgcg cctcgacgcc tttgtcacga
     1321 tgtccgggtc tgagcagccc gcctccccga ccaacttc
//
LOCUS       DAOSCR010000697         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_66666_length_1347_cov_3.115325, whole genome shotgun sequence.
ACCESSION   DAOSCR010000697 DAOSCR010000000
VERSION     DAOSCR010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66666_length_1347_cov_3.115325"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLT69_12875"
     CDS             <1..885
                     /locus_tag="PLT69_12875"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ15380.1"
                     /translation="VQADGKRRCAGEMVSYVRPVTELIRARRSWRTYQASRPRPEDAE
                     QIRSFIAGLGRPPFGSRVRIVLAELGHTVGDRVKGTYGVISGARSFLVGAMDQSESRP
                     EDFGYLFESVILCATSLGYGTCWVGGTFDRSAFADAVGLAGRETVCAVSPVGVPAKAR
                     STIDRLFAATAGSHTRKPFAELFFMDDFESPMNERSAGSHLAALEMVRLAPSSSNRQP
                     WRVLVKDGAYHFYLRRTPLYSLLFPKVDLQRLDMGIAMFHLEQTLCEQGVQGSWRTAA
                     PSGDVRAGAMEYVASFVP"
     gene            930..>1347
                     /locus_tag="PLT69_12880"
     CDS             930..>1347
                     /locus_tag="PLT69_12880"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HOJ15381.1"
                     /translation="MQRSGPEHRHRGEVVSGRNALEEVFRSGRRTVYEVFALEESAPR
                     WRDTPWHQALRVVDRRELDRIAGTREHQGVAARVSDYPYAALEDLFGLDVVLVLDSVE
                     DPQNLGAAIRAAHALAGAGVVIPRHRACPVTPAVVRA"
BASE COUNT          213 a          438 c          491 g          205 t
ORIGIN      
        1 gtccaagccg atggcaagag gaggtgcgcc ggggagatgg tgtcgtacgt acggccggtg
       61 acggagctca tccgcgcgcg gcgctcgtgg aggacctacc aggcctcccg gcccaggccc
      121 gaggacgccg agcagatccg ttctttcatc gccgggctcg gcaggccgcc gttcggctcg
      181 agggtgagga tcgtgctcgc cgagctcggt catacagtcg gcgacagggt aaagggcacc
      241 tacggcgtga tctcgggcgc aaggagcttt cttgtgggcg cgatggacca gtccgagtcc
      301 aggcccgaag acttcgggta cctcttcgag tcggtgattt tgtgcgcgac ctccctgggg
      361 tacggaacgt gctgggtcgg cgggaccttc gacaggtcgg ccttcgccga cgccgtgggc
      421 cttgccggga gagagacggt ctgcgccgtc agcccggtgg gggtgccggc caaggcccgc
      481 tcgaccatcg acaggctgtt tgcggccacg gccgggtcgc acacgaggaa gcccttcgcg
      541 gaactcttct tcatggacga tttcgaaagc cccatgaacg agcgctcggc cgggtcccac
      601 ctcgcggccc tcgagatggt ccgcctcgcg ccctcctcgt cgaaccggca gccgtggagg
      661 gtgctcgtga aggacggggc ataccacttc tacctcagga ggacgcccct gtactcgctc
      721 ctgttcccca aggtggacct ccagcggctg gacatgggga tcgccatgtt ccacctcgag
      781 cagaccctgt gcgaacaggg ggtccagggc tcctggcgca ccgccgcccc gtcgggggac
      841 gtccgcgccg gggccatgga gtacgtcgcg agcttcgtgc catgaaaccg aggcccccga
      901 agaaaccgtg gcggcgcccg gagcccgccg tgcaacgctc gggtcccgag caccgtcaca
      961 ggggagaggt cgtctcgggc agaaacgcgc tcgaggaggt gttccgctcc ggcaggagga
     1021 cggtctacga ggtcttcgca ctggaggaaa gcgccccgag atggcgggac acgccgtggc
     1081 accaggcgtt gagggtcgtg gacaggcgcg agctcgacag gatagccggg acgagggaac
     1141 accagggggt ggcggccagg gtctcggact atccgtacgc ggccctggaa gacctcttcg
     1201 ggctcgacgt cgtcctcgtg ctcgactccg tcgaggaccc gcagaacctc ggggccgcga
     1261 tcagggcggc gcacgcgctg gcaggcgccg gggtagtgat accgagacac cgggcgtgcc
     1321 cggtcacccc ggccgtggtc agggctt
//
LOCUS       DAOSCR010000698         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_66868_length_1344_cov_3.207137, whole genome shotgun sequence.
ACCESSION   DAOSCR010000698 DAOSCR010000000
VERSION     DAOSCR010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66868_length_1344_cov_3.207137"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1344)
                     /locus_tag="PLT69_12885"
     CDS             complement(<1..>1344)
                     /locus_tag="PLT69_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ15382.1"
                     /translation="GIPKRDLTLEDPNCVFQLMKKHYSRYTLKLVADICGGDPKDYEQ
                     VYDTFTATYKPDKSAVIMYAMGTTQHTYGSQNVRAFAMIQLLLGNIGVLGGGVAALRG
                     ESNVQGSTDHGALFHILTGYLKAPMASQQTLQKYLEDNTPKSNDPKSANWWGNTPKYT
                     VSLLKAWWPDVPHDQAYVYLPKNQDGVNYSIIPLYGAMASGKIKGLFCWGMNPAVSTP
                     SARMVRRALAKLDWLVAVDLWETETSAFWQKDAGVNPADIKTEVFLLPAASSVEKEGS
                     ISNSGRWAQWRYKAIDAPGHAEDDLWIMTQIVKRLKDMYAKEGGAFPDPILNLGWKVG
                     KLSKPSSREVAKEINGYFLVDKDIEDKATGEIKKFKAGDMVPGFALLQDDGSTCSGCW
                     IYCNSVTNTENKMMKRDNSDPSGMGLYHNWAWCWPVNRRILYNRAGVDANGKPSA"
BASE COUNT          195 a          418 c          434 g          297 t
ORIGIN      
        1 ccgcagaagg cttgccgttc gcatcgacac cggcccggtt gtagaggatc ctgcggttga
       61 ccggccagca ccaggcccag ttgtggtaca ggcccatgcc ggaggggtcg gagttgtcgc
      121 gcttcatcat cttgttctcc gtgttcgtca cggagttgca gtagatccag cagccgctgc
      181 aggtgctgcc gtcgtcctgg agcagcgcga agcccggcac catgtcgccc gccttgaact
      241 tcttgatctc gccggtcgcc ttgtcctcga tgtccttgtc cacgaggaag tagccgttga
      301 tctccttggc gacctccctc gacgagggct tgctgagctt gccgaccttc cagcccaggt
      361 tgaggatggg atccgggaag gccccgcctt ccttggcgta catgtctttg aggcgcttga
      421 cgatctgggt catgatccag aggtcatcct cggcgtgccc cggtgcgtcg atggccttgt
      481 agcgccactg cgcccagcgg ccggagttcg agatggaacc ctccttctcc accgaggatg
      541 cagccgggag caggaagacc tcggtcttga tgtcggccgg gttcaccccc gcatccttct
      601 gccagaaggc gctcgtctcg gtctcccaga ggtccaccgc cacgagccag tcgagcttgg
      661 ccagcgccct cctcaccatg cgtgcgctcg gcgtgctcac cgccgggttc atgccccagc
      721 agaacaggcc cttgatcttg ccgctcgcca tggcgccgta gagcgggatg atcgagtagt
      781 tgacgccgtc ctgattcttg ggcaggtaga cgtacgcctg atcgtgcggg acgtccggcc
      841 accaggcctt gagcaggctg accgtgtact tcggggtgtt gccccaccag ttggcgctct
      901 tggggtcgtt gctcttgggc gtgttgtcct cgaggtattt ctgtaacgtc tgctgggacg
      961 ccataggcgc cttcaggtat cccgtcagga tgtggaacag ggcgccgtgg tcggttgagc
     1021 cctgcacgtt gctctcgccc ctgagcgcag ccacccctcc gcctaggacg cctatgtttc
     1081 cgagcagcag ctggatcatg gcgaaggcgc gcacgttctg tgagccgtag gtgtgctggg
     1141 tcgtgcccat tgcgtacatg atcaccgccg acttgtcggg cttgtaggtg gcggtgaagg
     1201 tgtcgtagac ctgctcgtag tccttggggt cgccgccgca gatgtcggcc acgagcttga
     1261 gcgtgtagcg ggaatagtgc ttcttcatga gctggaacac gcagttcggg tcctcgaggg
     1321 tgagatccct cttggggatg ccgt
//
LOCUS       DAOSCR010000699         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_66877_length_1344_cov_2.855702, whole genome shotgun sequence.
ACCESSION   DAOSCR010000699 DAOSCR010000000
VERSION     DAOSCR010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66877_length_1344_cov_2.855702"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..>1344)
                     /locus_tag="PLT69_12890"
     CDS             complement(209..>1344)
                     /locus_tag="PLT69_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15383.1"
                     /translation="YIARHHAGARLGNDYATGAYLMWASKGRTKVFIDARYFPYKTWY
                     DEYAAFEEEENQASFEELAEGYGCDVWCVSIDSPLAWRFLSSSRWALAMYGRSAAVFV
                     RPGEVRAMVRPDAERLAEGPGHYRKIELARFALRAGDDKTAAIVSRGACRDVAGSVRP
                     CLSAETALVVGDSLARKRRYREAVQVLRSGAKCTEGLLPAVFARWDGCNARVAELHVH
                     LGMALLAMMRPAEAATEFRKALAIEPGRVDASRLLAETVKRLDALSRTAAGLEEEARR
                     RPSDLDVHRRLAAVLVMRGDLNRAYDISFALAKADPRHPEDLYNLACIQAMRGSTQEA
                     LEYLGRALDAGLPDPTIIETDPDLDPIRDTRAFRELLSRSKKG"
BASE COUNT          221 a          471 c          431 g          221 t
ORIGIN      
        1 ccacctacgt catcatccct gccccgggcg cggcaagaga cagcattcgc gggccaggcg
       61 catgggggag gaggggtatg gtttcggcaa gagggggggg tggtatgctc gcgggcaagg
      121 ctcatggggg acaaggattt accagaactt tcccatccaa aacattccac ttgcgcacca
      181 ggcgcatgcg ggacaacgac cttcggcgtc accccttctt cgagcgggac aggagctccc
      241 tgaaggcgcg ggtatccctg atggggtcga gatcagggtc cgtctcgatg atggtggggt
      301 ccggcaggcc tgcatccagg gccctcccca ggtactcgag cgcctcctgc gtgctgccgc
      361 gcatggcctg tatgcaggcg aggttgtaca ggtcctcggg atgtctcggg tcggccttcg
      421 caagggcaaa ggagatatcg taggccctgt tgagatcgcc ccgcatcacc aggacggctg
      481 caagtctcct gtgcacgtcc aggtcggaag gcctcctcct cgcctcctcc tcgaggcccg
      541 cggccgtcct ggacaaggca tcgagccgct ttacggtctc ggcgaggagc ctcgaggcgt
      601 ccacgcggcc gggctcgatc gcgagcgcct tcctgaactc cgtagccgcc tcggcaggcc
      661 gcatcatggc caagagcgcc atgccgaggt gcacgtgcag ctcggccaca cgggcgttgc
      721 acccgtccca cctggcgaac acggcgggca gcaggccttc tgtgcacttc gctcccgagc
      781 gcagcacctg caccgcctcc cggtacctcc gcttgcgcgc cagggagtcg cccacgacaa
      841 gggcggtctc ggcgctcagg cacggtcgta ccgagccggc cacgtccctg catgcgcccc
      901 tggagacgat cgccgccgtc ttgtcgtccc cggccctcag agcgaacctc gcgagctcga
      961 tcttccggta atgaccgggc ccctctgcga gcctttccgc atccggccgc accatggccc
     1021 gaacctcgcc gggcctgacg aacacggcag cagagcggcc gtacatggcg agggcccacc
     1081 tcgaagacga gaggaaccgc cacgcgagcg gggaatcgat ggagacgcac cacacgtcgc
     1141 acccgtaccc ctccgccagc tcctcgaacg acgcctggtt ctcctcttcc tcgaaggccg
     1201 cgtactcgtc gtaccaggtc ttgtagggga agtacctcgc gtcgatgaac accttggttc
     1261 tgccctttga cgcccacatg aggtaggcgc cggtcgcgta gtcgttccca aggcgcgccc
     1321 cggcgtgatg ccgggcgatg tagc
//
LOCUS       DAOSCR010000700         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_67030_length_1342_cov_2.592075, whole genome shotgun sequence.
ACCESSION   DAOSCR010000700 DAOSCR010000000
VERSION     DAOSCR010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67030_length_1342_cov_2.592075"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1342)
                     /locus_tag="PLT69_12895"
     CDS             complement(<1..>1342)
                     /locus_tag="PLT69_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15384.1"
                     /translation="MGRLFEKSPLVELAGVVRMQEGTACILSVHEPYRLKRARRDDLR
                     GAVVLELAPSKTASKRTMEDLGLKLLEAGDYASAISILEPVSPSLGFSGLTTLARAYW
                     AASYPYAKGETASKAFSIMDRAVTAAAAGPEAEAAALEYCSWLVASGRTKDASRMLGG
                     LEGSSDPAVRIHAMILAMAALNRDGSYDDAYALSHKLSSHKDMPERLKGLFLATLADT
                     LLGLKDFARAHDQYLRALAMDPGSARSDPGVFGRMGEAALKMNDFASARVHLTDAVNL
                     AAASDRHRFLVMLGDAFYELGEKDRSAAAFLQAEQAASTTGELSLAMLKRARIVAEKH
                     TDERGRLSDKGYNEVMDIYASIEENLRKDKPDDALLRLVKVREAQAYARHQEIDRALA
                     TYLEVWKGGKRDDDLGRYARAEAMRIIIEQCRIYHRDNRYDKTLELYLRFKDSFV"
BASE COUNT          196 a          434 c          451 g          261 t
ORIGIN      
        1 gacgaaggag tccttgaacc tgaggtagag ctcgagcgtc ttgtcgtacc tgttgtcacg
       61 gtggtatatc ctgcactgct cgatgatgat gcgcatcgcc tcggcccgtg cgtacctgcc
      121 gaggtcgtcg tccctcttcc cgcccttcca cacctcgagg taggtcgcga gagcccggtc
      181 gatctcttgg tggcgggcat aggcctgtgc ctctctcacc ttcacgagcc tgaggagcgc
      241 atcgtcgggc ttgtccttcc tgaggttttc ctcgatggag gcatagatgt ccatgacctc
      301 gttgtacccc ttgtcggaaa gccgcccacg ctcgtcggtg tgcttctccg ccacgatgcg
      361 ggccctcttg agcatggcca gggagagttc accggttgtg gacgcggcct gttccgcctg
      421 caggaaggcg gccgccgagc ggtccttctc gccgagctca tagaaggcgt cgccgagcat
      481 gacgaggaac ctgtgccgat cgctcgcggc tgcgaggttg accgcgtccg tcaggtgaac
      541 cctggccgag gcgaagtcgt tcatcttcag ggccgcctcg cccatcctcc cgaagacgcc
      601 cgggtcggac ctggcgctgc ccgggtccat ggcgagggcc ctgaggtact ggtcgtgggc
      661 gcgggcgaag tccttgagac cgaggagggt gtccgccagg gtggcgagga agagcccctt
      721 cagcctctcg ggcatgtcct tgtgggagga gagcttgtga gacagcgcat aggcgtcgtc
      781 gtacgacccg tcccggttca acgccgccat ggcgaggatc atggcatgga tccggaccgc
      841 tgggtcggag gacccctcga gtccgcccag catgcgcgag gcgtcctttg tcctcccgga
      901 ggcgacgagc cacgagcagt attcgagcgc ggccgcttcc gcctcgggac ctgccgcagc
      961 agcggttacg gcgcggtcca tgatggagaa ggccttcgat gcggtctcgc ccttggcgta
     1021 ggggtacgat gccgcccagt acgcccttgc cagggtggtc aggccgctga acccgagcga
     1081 gggggagacg ggttcgagga tgctgatcgc ggatgcgtag tcccccgcct cgaggagttt
     1141 caggccgagg tcctccattg tgcgttttga tgcggtcttc gagggcgcga gctcgaggac
     1201 gaccgctccc ctcaggtcgt cccttcgcgc cctcttgagc ctgtacggct cgtgcacgct
     1261 gaggatgcag gccgtcccct cctgcatcct caccacgccg gcgagctcca ccagggggga
     1321 tttctcgaac aggcgcccca tg
//
LOCUS       DAOSCR010000701         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_67657_length_1334_cov_2.394058, whole genome shotgun sequence.
ACCESSION   DAOSCR010000701 DAOSCR010000000
VERSION     DAOSCR010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67657_length_1334_cov_2.394058"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLT69_12900"
     CDS             <1..197
                     /locus_tag="PLT69_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15385.1"
                     /translation="TNCLLNALANHLHRKRFSFHPYAWEIAGIVRSGCMDRETGLAKV
                     TEEEDEVMVRRAADLLGIGT"
     gene            complement(332..>1334)
                     /locus_tag="PLT69_12905"
     CDS             complement(332..>1334)
                     /locus_tag="PLT69_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOJ15386.1"
                     /translation="EKLLGPMKGKRKVLILTHENPDPDSIAAAFGLKYLIRRLAGIPS
                     VIAYGGIIGRAENRAMVKHLAIDLMPLSKVSARNYSVIAAVDCQPHTGNLMLPKGVFP
                     NIIIDHHPLRKTSLRADYHDIARDVGSTSTIITHYLRTLGIEPDRKVATALYYGIKSD
                     TRDLGRQSTDNDIRASTYLFPHVLLKKLSRIEHPDLPRQYFQELTNVLTNAHVYGDVV
                     VARLEFMSWPEMIGEFADELVQMEGVRWCLCYGRHNGYLLFSIRAKRTKDMAGILAHK
                     IAVGLGTGGGHETFAAGKIDVKRFLKEVNTKGAEPVPLVDPSQMTCRVRREPMAPDT"
BASE COUNT          202 a          408 c          454 g          270 t
ORIGIN      
        1 ccacgaactg cctcctgaac gcgcttgcga accacctgca cagaaagcgg ttctcgttcc
       61 acccctacgc atgggagatt gcgggcatcg tccgctcggg gtgcatggac cgggagaccg
      121 gcctggccaa ggtgaccgag gaggaagacg aggtcatggt caggcgggcg gcggatctcc
      181 tgggtatcgg cacctgacgg ccccggcttg accctcgaga tcgtccggga cgggtacacg
      241 aggggcacag gctcggcccc cttcggtgtt cacctccttg gggggcgccc ggcggtgatg
      301 acgcctcgtt cgcggccggg cggctggtct ctcaggtgtc aggggccata ggttcccgcc
      361 tgaccctgca ggtcatctgg gacgggtcca cgaggggcac gggctcggcc cccttggtgt
      421 tcacctcctt gaggaagcgc ttgacgtcga tctttccggc cgcgaaggtc tcgtggcctc
      481 ctcctgtccc cagcccgacg gcgatcttgt gcgcgaggat acccgccatg tccttggtgc
      541 gcttcgccct gatggagaag agcaggtacc cgttgtgcct gccgtagcac aggcaccacc
      601 tcacgccctc catctgcacg agctcgtcgg cgaactcccc tatcatctcg ggccagctca
      661 tgaactcgag cctggcgacc acgacgtcac cgtagacatg cgcgttggtg agcacgttgg
      721 tgagttcctg gaagtattgc ctcggcaggt cggggtgttc gatgcgggag agcttcttga
      781 ggagcacgtg ggggaagagg taggtcgagg ccctgatgtc gttgtccgtg gactgcctgc
      841 caaggtccct cgtgtccgac ttgatcccat agtacagggc ggtcgccacc ttccggtccg
      901 gctctatgcc gagcgtcctc aggtagtgcg tgatgatggt cgaggtgctg cccacgtccc
      961 tggctatgtc gtggtaatcc gccctgagcg aggtcttccg cagggggtgg tggtctatga
     1021 tgatgttggg aaacaccccc ttgggaagca tgaggttgcc ggtgtgcggc tggcagtcca
     1081 cggccgcgat caccgagtag ttgcgggccg agaccttgga aagcggcatg aggtcgatcg
     1141 cgaggtgctt gaccatggcc ctgttctcgg ccctgccgat gatgccgccg taggcgatca
     1201 cgcttggtat gcccgcgagc ctccggatga ggtacttgag cccgaaggcc gcggctatgc
     1261 tgtcggggtc cggattctcg tgggtgagga tgagcacctt tcgcttgccc ttcatgggcc
     1321 caaggagctt ctcg
//
LOCUS       DAOSCR010000702         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_67695_length_1333_cov_3.411581, whole genome shotgun sequence.
ACCESSION   DAOSCR010000702 DAOSCR010000000
VERSION     DAOSCR010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67695_length_1333_cov_3.411581"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLT69_12910"
     CDS             complement(<1..640)
                     /locus_tag="PLT69_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ15387.1"
                     /translation="MRGLASKGKMDYILAMFEHLPSLVFADAEGRVMDFPDLAMVARS
                     GDYLLPAAKEEVIPLPEGSQLYVLPERMPVGLDRKTGEVVVLESNPYEASSPVSAVAV
                     FLPAAYTQTYLAAWERSHRACELPLLAYTALGWQDGFVTTAVRTDPSTRQDPNTFDRE
                     KIEKGILQWLQDLPGNRLVEHLVSCATCYGCPAALNLFQGREEAPLPTSPSCN"
     gene            803..>1333
                     /locus_tag="PLT69_12915"
     CDS             803..>1333
                     /locus_tag="PLT69_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15388.1"
                     /translation="MCEHNGNIYTPRPVTIARTLPQIEHHRLFQLSLEDGSSWEEFGH
                     QPGQFIEVSVFGKGEAPISISSPPTRCDHLEICVRRAGKVTGAMFEMGKGSRLSIRGP
                     YGRGFPVETFKGHKLLFVAGGLGLAPLRSLLLYVLDKRKDFDDLILMYGTNRPDNVLF
                     KYELLSFFDRDDIKYLY"
BASE COUNT          257 a          415 c          414 g          247 t
ORIGIN      
        1 cgttgcagga cggggacgtg ggcagggggg cctcctccct cccctggaag agattcaagg
       61 ccgccgggca cccgtaacag gtcgcgcacg acacgaggtg ttccacgaga cggttaccgg
      121 gcaggtcctg gagccactgg aggataccct tctctatctt ctccctgtcg aaggtgttcg
      181 ggtcctgcct ggtggacggg tccgtcctca ccgcggtcgt cacgaacccg tcctgccagc
      241 ccagggccgt gtaggcgagc aggggcagct cgcacgccct gtgcgaacgt tcccaggccg
      301 cgaggtaggt ctgcgtgtac gccgcgggga gaaagacggc cacagccgat acgggcgagg
      361 acgcctcgta cgggttcgac tcgagcacga ccacctcccc tgtcttgcgg tcgaggccca
      421 ccggcatcct ttcggggagc acgtagagct gggagccctc gggcagaggg atgacctctt
      481 ccttagccgc gggaaggagg tagtccccgc tccttgcaac catggcgaga tcggggaaat
      541 ccatgaccct gccctctgca tccgcaaaga cgaggctggg gagatgctcg aacatggcga
      601 ggatgtagtc catctttccc ttggacgcaa gacctctcac gtgcggggta aacctccccc
      661 gcgttgggga aagttgcccc atgcaccggt tcctgcagca taccggctgt cgatacagcc
      721 agtgttttac ggtgactacg atcatggcac cgatcttgcg aagattttcc cgtgagatga
      781 gtatatccgc gaggaggttc gcatgtgtga gcacaacggg aacatctaca ctccccggcc
      841 ggttaccatc gccaggacgc tcccacagat agaacaccac cgcctgttcc agctcagcct
      901 ggaggacggg tcttcatggg aagaattcgg tcaccagccc ggacagttca tcgaggtctc
      961 cgtcttcggc aagggcgagg cgcccatcag catatcatcg ccgcctacca ggtgcgacca
     1021 cctcgagatc tgtgtgaggc gtgcaggcaa ggtgacaggg gcgatgttcg agatgggcaa
     1081 ggggtcgagg ctctccatca ggggaccgta cggcagaggt tttcctgtcg agacgttcaa
     1141 gggccacaag ctcctcttcg tggccggagg tctcgggctt gcgcccctga ggtcgctgct
     1201 cctctacgtg ctggacaaga gaaaggactt cgacgacctc atcctcatgt acggcaccaa
     1261 caggccggac aacgtcctct tcaagtacga gctcctgagc ttcttcgacc gggacgacat
     1321 caagtacctc tac
//
LOCUS       DAOSCR010000703         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_68220_length_1326_cov_4.251770, whole genome shotgun sequence.
ACCESSION   DAOSCR010000703 DAOSCR010000000
VERSION     DAOSCR010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68220_length_1326_cov_4.251770"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLT69_12920"
     CDS             complement(<1..228)
                     /locus_tag="PLT69_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ15389.1"
                     /translation="MKREEKAHIVQVLHDELQRAQAVFVTDYMGLNVERITRLRRDVA
                     NSGGSYRVVKNTLLRRATGGTNADVVSDLFVG"
     gene            complement(361..1065)
                     /gene="rplA"
                     /locus_tag="PLT69_12925"
     CDS             complement(361..1065)
                     /gene="rplA"
                     /locus_tag="PLT69_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ15390.1"
                     /translation="MARSGKKYREARKKIDPQKKYPLGDALGLVLDSSFAKFDESVDV
                     ALRLGVDPRHADQMVRGSVVLPHGTGKTVRVLVFAKGEKEKEARDAGADYVGAEDLAE
                     KIQGGWLEFDKVIATPDLMGMVGKLGKILGPRGLMPNPKVGTVTFDVAKAVADMKAGR
                     VDFRVDKVGIVHCSVGKVSFGKEKILDNVKALLEMVMKLKPASSKGTYLKGFAVSSTM
                     GPGVRIDTVEAANLLK"
     gene            complement(1085..>1326)
                     /locus_tag="PLT69_12930"
     CDS             complement(1085..>1326)
                     /locus_tag="PLT69_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOJ15391.1"
                     /translation="DRSFTFVTKSPPASTLLLKAAGIEKGSQAPNKDKVGKVTRAQVM
                     DIARQKMNDLNAQDMDNAFKIIAGTARSMGIEIVD"
BASE COUNT          201 a          453 c          367 g          305 t
ORIGIN      
        1 ccccacgaac agatccgaga cgacatccgc gttcgtgccg cccgtggccc gcctgagcag
       61 ggtgttcttg accacgcgat aggacccgcc cgagttggcg acgtcccgcc tgagcctggt
      121 gatcctctcc acgttgagcc ccatgtagtc cgtcacgaag acggcctgtg cacgctggag
      181 ctcatcgtgc aggacctgta cgatgtgtgc cttctcttcc ctcttcaatg aaacacctca
      241 cccctttctc ttttggattc atcctgtcaa agggtgcctc ggggtatccg tgcccgggtc
      301 tcggcgggaa tgtttaagcg caaaagcggc gctcccgctg tctttgacca ggatcttttc
      361 tcacttcaga agattcgcgg cctctaccgt gtctatcctc acgcccggcc ccatggtgct
      421 ggacaccgcg aaccccttga gataggtgcc cttggacgac gcgggcttga gcttcatgac
      481 catctcgagc aacgccttga cgttgtcgag gatcttctcc ttgccgaagg acaccttgcc
      541 caccgagcag tggacgatgc ccaccttgtc gaccctgaag tccactcggc ccgccttcat
      601 gtccgccacc gccttggcga cgtcgaaggt gaccgtaccc accttggggt tgggcatgag
      661 gccgcggggg ccgaggatct ttcccagctt gcccaccatg cccatgaggt cgggcgtggc
      721 gatgaccttg tcgaactcaa gccagccgcc ctgtatcttt tccgcgaggt cctccgcccc
      781 gacgtaatcg gcgcctgcgt ccctggcttc cttctccttc tcgccctttg cgaacacgag
      841 gaccctcacg gtcttcccgg taccgtgggg gagcaccacc gaaccgcgga ccatctggtc
      901 ggcgtgcctg gggtcgaccc cgagcctgag ggccacgtcc acgctctcgt cgaacttcgc
      961 gaaagaactg tccaacacca gccccagggc gtcaccgagg gggtacttct tctgcggatc
     1021 gatcttcttc ctcgcctctc tgtatttctt accgcttctg gccatgttcg cgtttctcct
     1081 tcactcagtc gacgatctcg atccccatgc tccgggcggt gcctgcgatg atcttgaagg
     1141 cgttgtccat gtcctgggcg ttgaggtcgt tcatcttctg ccgggcgatg tccatgacct
     1201 gcgccctggt gacctttccc accttgtcct tgttgggcgc ctgggagccc ttctctatcc
     1261 cggcggcctt gaggagcagg gtggatgctg gcgggctctt ggtcacgaag gtaaacgacc
     1321 gatcct
//
LOCUS       DAOSCR010000704         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_68231_length_1326_cov_2.894571, whole genome shotgun sequence.
ACCESSION   DAOSCR010000704 DAOSCR010000000
VERSION     DAOSCR010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68231_length_1326_cov_2.894571"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="dnaX"
                     /locus_tag="PLT69_12935"
     CDS             complement(<1..937)
                     /gene="dnaX"
                     /locus_tag="PLT69_12935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ15392.1"
                     /translation="MYHVIARKFRPQRFEDVVGQEHVTKVIGNAIELQRLHHAFIFSG
                     PRGVGKTSVARILARAINCERGMSSTPCNECTLCQDITASRAVDVFEIDAATHTQVDK
                     IRDLIENARYAPAVARYKTFIIDEVHMLSRSAFNALLKTLEEPPPHVVFILATTEPAK
                     VPLTVLSRCQRYDFRRIPVEGIVGRLREVAQREGIPVDEGALVTIALQADGSMRDAQG
                     MLEHVWSASTGLIDSDAVSKAFGLVGRATMRDLVAALVKKDAPVVLDVVERVHEGGQD
                     LAQLYRSLVEYVRNMMVIKAGFACRGLPQEDALFLR"
BASE COUNT          216 a          449 c          421 g          240 t
ORIGIN      
        1 ctctcaggaa caaggcgtcc tcctgcggca gccccctgca ggcgaacccc gccttgatga
       61 ccatcatgtt cctcacgtat tccacgaggg accggtagag ttgcgcaagg tcctgccccc
      121 cctcgtgcac cctctccacc acgtcgagca ccacgggggc gtccttcttg acgagcgcgg
      181 ccacgaggtc gcgcatggtc gccctaccca cgagcccgaa cgccttcgag accgcgtctg
      241 agtctatgag gcccgtggaa gccgaccaga cgtgctcgag catgccctgg gcgtccctca
      301 tgctgccgtc cgcctgcagc gctatggtca cgagagcgcc ctcgtccacg gggatgccct
      361 ccctttgcgc aacctcgcgc agcctgccca cgatcccctc gaccgggatc ctcctgaaat
      421 cgtagcgctg gcagcgggac agcaccgtca gcggcacctt ggccggctcc gtcgtcgcga
      481 ggatgaagac cacgtgcggg gggggctcct cgagggtctt cagcagggcg ttgaaggcgc
      541 tgcgcgacag catatgcacc tcgtcgatga tgaacgtctt gtaccgggcg actgcgggcg
      601 cgtacctcgc gttctcgatg aggtcccgta tcttgtccac ctgggtgtgc gtcgcggcgt
      661 cgatctcgaa cacgtctacg gccctcgatg ccgtgatgtc ctggcacaac gtgcactcgt
      721 tgcacggggt gctcgacatc ccgcgttcgc agttgatggc gcgcgcgagg atgcgggcga
      781 ccgacgtctt cccgacaccc ctcgggccgg aaaagatgaa ggcgtggtgc agcctttgga
      841 gctcgatggc gttgcctatg accttggtca cgtgttcctg gccgacgacg tcttcgaatc
      901 tctgaggtct gaatttcctg gctatgacat gatacatccg tgcatcaccg ttcgagaggg
      961 atcgatcatc accgtatcac gagcgacccg cctgttcaac gcccttcggt gtgatcgcgt
     1021 gaagaggagg cgagcaacgg ggccttgcgc cgtcacgcgg tctttccggc aggggtgaac
     1081 gccgcgtggg tgccctgccc tcgaggttcg gcataaaacc gcccttgtct ccaggggtta
     1141 ccggttggcc gcgtcgcgag aggcacgcaa gagcgagctt cacggtgggg caggccctcc
     1201 tccagcacgc cgtggggata agcggcctcc ccgcatgggg gaaggccttg cagagggtgc
     1261 cccgagggca gcaatgaacc gagaccgccg gcacccctct caggccgacc gtgcctcaac
     1321 cggggc
//
LOCUS       DAOSCR010000705         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_68447_length_1323_cov_3.242114, whole genome shotgun sequence.
ACCESSION   DAOSCR010000705 DAOSCR010000000
VERSION     DAOSCR010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68447_length_1323_cov_3.242114"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLT69_12940"
     CDS             complement(<1..467)
                     /locus_tag="PLT69_12940"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOJ15393.1"
                     /translation="MNRASFFEGIVQWELTNEKLGFSGKLPVFYYDNTSMTVTYTAPT
                     AMVRRLLPRADMHPVEIVRGKALVAFTAFEYRSTDIDPYNEFSISFPITFGKKGIPGL
                     TALSMMARRYFTAYVWQLPVTTERARIGGVEMYGYPKFLADITFSDERDMLAC"
     gene            complement(567..>1323)
                     /locus_tag="PLT69_12945"
     CDS             complement(567..>1323)
                     /locus_tag="PLT69_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ15394.1"
                     /translation="ALADIDHGGLEQTAALVDGRVRVTTHVVDVSRRERVFAFAQDAV
                     SLHGSVDLVINNAGVALGDLLETVPIEDFEWIMAVNFWGVVHGTMAFLPHLRLRPEGH
                     IVNVSSINGILPNPNNGPYCASKFAVKGYTETLAQELEGTNIRVSCVHPGGIRTGIAR
                     HAKFNRGLCSLTKEGAVSLYEKELFRMDAGEAARVIIAGIKRDRRRILVGTDALVLDM
                     LTRLFPVTALRLCSAFSRYMARKYACTGDGNTG"
BASE COUNT          227 a          431 c          407 g          258 t
ORIGIN      
        1 ctgcaggcga gcatgtctcg ctcgtcggag aaggttatgt cagcgaggaa cttggggtac
       61 ccgtacatct cgaccccgcc gatacgtgcg cgttcggtcg tcacggggag ctgccacacg
      121 taggcggtga aatacctcct cgccatcatg gagagggcgg taagccccgg tatgcccttc
      181 ttcccgaagg tgatggggaa cgagatggag aactcgttgt acgggtcgat gtcagtcgac
      241 cggtactcga atgcggtgaa ggcgaccagc gccttgcccc tgacgatctc caccgggtgc
      301 atgtccgccc tcggcagcag ccgcctcacc atggccgtgg gcgcggtgta ggtgaccgtc
      361 atgctcgtgt tgtcgtagta gaagaccggc agcttgcccg aaaaccccag tttctcgttg
      421 gtgagttccc attgcacgat cccttcgaag aacgaagccc tgttcataga tcctcccttt
      481 cgatcccccg ggggtctggc cccgggtcat agtcgtctcg agcctggaca cgtttcccct
      541 cgatgaccca atgccctccc tgcagcctaa ccggtatttc catcccctgt gcaggcgtac
      601 ttgcgggcca tgtacctgct gaaggcggaa cacagtctca gagccgtcac gggaaagagc
      661 ctcgtgagca tatccaggac caaggcgtcc gtcccgacga ggatgcggcg ccggtccctc
      721 ttgatgccgg cgatgatgac gcgggcggcc tcgccggcat ccatcctgaa gagctccttc
      781 tcgtagaggc tcacggcccc ctccttcgtc agggagcaca acccgcggtt gaacttggcg
      841 tggcgggcga tccccgtcct gatgcccccc gggtgtacgc agctcacgcg tatgttcgtg
      901 ccctcgagtt cctgggcgag ggtctcggtg tatcccttga ccgcgaactt ggatgcacag
      961 tacgggccgt tgttgggatt gggcaggatc ccgttgatgc tggacacgtt cacgatgtgc
     1021 ccctcgggcc tcagcctcag gtgggggaga aacgccatgg tcccgtggac cacgccccag
     1081 aagttcacgg ccatgatcca ctcgaagtcc tctatcggga cggtctctaa aaggtcgccg
     1141 agggcgacgc ccgcgttgtt gatgacgagg tccaccgaac catgcaggct cacggcatcc
     1201 tgcgcgaacg cgaagacccg ctccctcctg gagacgtcca ccacgtgcgt ggtgaccctc
     1261 acccggccgt ctacgagggc cgcggtctgc tcgagacccc cgtggtcgat gtccgccagc
     1321 gcg
//
LOCUS       DAOSCR010000706         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_68895_length_1318_cov_2.303246, whole genome shotgun sequence.
ACCESSION   DAOSCR010000706 DAOSCR010000000
VERSION     DAOSCR010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68895_length_1318_cov_2.303246"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1318)
                     /locus_tag="PLT69_12950"
     CDS             complement(<1..>1318)
                     /locus_tag="PLT69_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15395.1"
                     /translation="VITDIYGRTQELAFSFIQETTLLAEGFSQFSCNVGVPRVTSAES
                     IVYEEDEAAFSLFYRRGLSDVFTSGAYVQGIRDRGLMGAVGTWATGAGVVNLDAALGF
                     ARHRDRGIAGKLAFVHRPRPTSTSPAVSLQLEAEYVGSGFCRPMDLFGNDGNDLRLAG
                     YVAVPLALGFSSSLGAGYTFTGGKDARDRYELSAGLRRLWFNKKVSTSLAVQGLRDVD
                     GGHEKRVFFGLVWMFPDEKQSVSAGMASGGSHDLQWDYGPASSSPGNVHAQVKVGRRS
                     EQESLRAKAGYVGNCGVVEVSQEVIHPSDPSQADVLNETTLSLGSSLVYVDGYWALSR
                     PVNQGFVLVKGVKNLEGVQVAVNPGPEGCQAVSSTYMPGVLPTLSPYNIRNLRVEPVD
                     PPEGFLPERTSYTLFPTYKSGYAITVGSDPTVIVVGVLRDRETGEPL"
BASE COUNT          234 a          464 c          381 g          239 t
ORIGIN      
        1 gagcggctct cccgtttccc tgtctcgcag caccccgacg acgatgaccg tggggtccga
       61 tcccacggtg atcgcatacc cggacttgta tgtcgggaag agcgtgtagc tggtcctctc
      121 cgggaggaac ccctccggcg ggtcgacggg ttcaacacgc aggttccgta tgttgtaggg
      181 ggagagcgtc ggaaggaccc ccggcatgta cgtgctcgag acggcctggc agccttccgg
      241 tcccggattg acggccacct gcaccccttc gaggttcttg acgcccttca ccagcacgaa
      301 cccctggttc accggccggg agagggccca gtacccgtcc acgtatacca gggagctccc
      361 caggctcagg gtcgtctcgt tcaagacatc ggcttggctt ggatcactgg ggtggatcac
      421 ctcctgcgac acctcgacca ccccgcagtt acccacatac cctgctttcg ctctcaggga
      481 ttcctgttcg ctccgccggc ctaccttcac ctgggcatgg acattccccg gcgaggagga
      541 ggcgggcccg taatcccact ggaggtcgtg gctccccccg gaggccatcc cggccgatac
      601 gctctgcttc tcgtcgggga acatccagac gaggccgaag aacaccctct tttcgtgccc
      661 cccgtctacg tccctcaacc cctgaaccgc gaggctcgtc gagaccttct tgttgaacca
      721 cagccgcctg agccctgcag agagctcata gcggtccctt gcgtccttgc cgcctgtaaa
      781 ggtgtaccct gcaccaaggc tcgacgagaa cccaagcgcc aggggcaccg cgacataccc
      841 ggccagacga aggtcgttcc cgtcgtttcc aaagaggtcc atgggtctgc aaaacccgga
      901 accgacgtac tccgcctcga gttgcaggct tacggccggc gaggtcgaag tcggcctcgg
      961 cctgtggacg aaggcgagtt tcccggctat gccccggtct ctgtgcctcg cgaaaccgag
     1021 tgcggcatcg aggttgacga cccccgcccc cgtcgcccag gtacccaccg cgcccatcag
     1081 ccccctgtcc cgtatcccct gcacgtacgc accggaggtg aagacgtcgc tcagccccct
     1141 ccggtagaac agggaaaagg cggcctcgtc ctcttcgtac acgatggatt ccgcggaggt
     1201 caccctcggc acacccacgt tgcacgaaaa ctgcgaaaac ccttcggcga ggagcgtcgt
     1261 ctcttggatg aaggaaaaag cgagctcctg tgtcctgccg tagatgtccg tgatgacg
//
LOCUS       DAOSCR010000707         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_68941_length_1317_cov_4.174326, whole genome shotgun sequence.
ACCESSION   DAOSCR010000707 DAOSCR010000000
VERSION     DAOSCR010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68941_length_1317_cov_4.174326"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PLT69_12955"
     CDS             complement(<1..1295)
                     /locus_tag="PLT69_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ15396.1"
                     /translation="MTPAQFEDKTRKNFLKKLFFSLISENVTVTSDEIEALYHLMNDT
                     YDLNYLVVSPQDFLASVQVGDDEVKKFFEENKERYRVPARITVTLVDFPTSRYLDQAR
                     VSEEEARDYFESHKGEFSRPEAVQLRNILITVPEGSDAKTQGEKTALAERIMAEIKAG
                     KDFAALAKTYSEDPTSGPKGGDLGEVPLGSLPEEIAKAVSGMQPGDVKGPFRSKNGLH
                     IVKLEARKAAKETPFEEVAQAIVGKLSTQRAKIVAREEAKKAFMELYESKKPDVEAFA
                     KARGLAPRIVGPASEEELKAAFIEPEAVKKAFTFATGELGEVVETPEGYALYQVSRKE
                     LSRIPELKDIAALVREDAKVKAAARQALEHARKLCETETARLVAMNPQSTGEFTRSAA
                     SVPGLESIKGLMDQVDDLTKPKAFDAGTKAYVVWIKSRKA"
BASE COUNT          191 a          436 c          395 g          295 t
ORIGIN      
        1 gccttcctgg acttgatcca cacgacgtac gcctttgtcc ccgcatcgaa ggccttgggt
       61 ttcgtcaggt cgtccacctg gtccatgagg cccttgatcg actcgaggcc gggaaccgag
      121 gccgccgatc tcgtgaactc gcccgttgac tgcgggttca tggccacgag ccgggcggtc
      181 tctgtctcgc acagcttgcg cgcatgttcc agggcctgcc tggcggccgc cttcaccttc
      241 gcgtcctcgc gcacgagcgc cgcgatgtcc ttgagttcgg ggatccggga cagctccttc
      301 ctgctcacct ggtacaacgc atacccctcg ggcgtttcca ccacctcgcc gagctcaccc
      361 gtggcgaagg tgaaggcctt cttgacagcc tcaggttcga tgaaggccgc cttgagttcc
      421 tcctcggaag ccgggccgac gatcctgggg gcaaggcccc gcgccttcgc gaaggcctcg
      481 acatcgggct ttttactctc gtagagctcc atgaaggcct tcttggcctc ctcgcgtgca
      541 acgatcttgg cacgttgggt cgagagcttc ccgacaatgg cctgggcgac ctcttcgaag
      601 ggtgtctctt tggcggcctt gcgcgcctcg agcttcacga tgtgaaggcc gttcttcgac
      661 ctgaagggcc ccttcacgtc gccgggttgc atcccgctca ccgccttcgc gatctcctcg
      721 ggcagcgagc ccaggggcac ctcgccgagg tctccgccct tgggaccgct cgtggggtct
      781 tcggaatagg tcttggccag ggccgcgaaa tccttgccgg ccttgatctc cgccatgatc
      841 ctctctgcga gcgccgtctt ttctccctgg gtcttcgcat cggacccttc ggggaccgtg
      901 atcaggatgt tcctgagctg caccgcttca ggacggctga actcgccctt gtgggactcg
      961 aagtaatccc gggcctcctc ctcgctcacc cgcgcctggt cgaggtagcg ggaggtcggg
     1021 aaatccacga gcgtgaccgt gatcctggcg ggcacccggt atctttcctt gttctcctcg
     1081 aagaacttct tgacctcgtc atccccgacc tggaccgagg caaggaagtc ctgggggctg
     1141 acgacgagat agttgaggtc gtaggtgtcg ttcatgaggt ggtacagggc ctcgatctcg
     1201 tcggaggtga ccgtcacgtt ctcgctgatg agcgagaaga agagcttctt caggaagttc
     1261 ttcctggtct tgtcctcgaa ctgcgcgggc gtcatcccct cgtactcgag gtatctc
//
LOCUS       DAOSCR010000708         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_68945_length_1317_cov_3.545959, whole genome shotgun sequence.
ACCESSION   DAOSCR010000708 DAOSCR010000000
VERSION     DAOSCR010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68945_length_1317_cov_3.545959"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLT69_12960"
     CDS             <1..435
                     /locus_tag="PLT69_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ15397.1"
                     /translation="LWADKLALMLKVRWGTVNPAKGDKLPEYERFDLGGLGSIRGFKW
                     GEVGPQDSLGNFIGGRRMVVMNIEATFPIGPIPGLYGVLFHDEGNCYDKRIDLTNLKK
                     SYGAGIRWVTPMGPLRLEYARVINPEDWEEPSRWEFSIGSFF"
     gene            472..1035
                     /locus_tag="PLT69_12965"
     CDS             472..1035
                     /locus_tag="PLT69_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOJ15398.1"
                     /translation="MKRHVTWVCILLTVLFVSCLPAGSRAEELKIACIDSRRVITESM
                     VGKDAYEKLKKLSEQKESELKKQEAKIKSLSDEIQAKSATMSASAKEDLQQRYEKEVK
                     EYNRSYKDAQDDLRNTEMNLLKPWTKELEAIIKDYGEKNKIDLILDKTNPAVIYGSKK
                     VDITDAIISLFDKRYQEKSSQQKQKKE"
     gene            1038..>1317
                     /locus_tag="PLT69_12970"
     CDS             1038..>1317
                     /locus_tag="PLT69_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOJ15399.1"
                     /translation="MRLSRITELLGGSLAGEDREITGASTLEEATPSDISFFANRKYR
                     EAALRTRAGALVTSEPLDAPCSQIVTDNPYLYFARVLGLLHPEPQRPRG"
BASE COUNT          310 a          399 c          406 g          202 t
ORIGIN      
        1 ctctgggcgg acaaactcgc cttgatgttg aaggtgagat ggggtacagt gaaccccgcc
       61 aagggcgaca agctccccga gtacgagcgg ttcgacctgg gcggcctcgg gagcatcagg
      121 gggttcaagt ggggggaggt cggtccccag gacagcctgg gcaacttcat cggcggcagg
      181 cggatggtgg tcatgaacat cgaggcgacc ttccccatag gaccgatccc cggcctctac
      241 ggcgtcctgt tccacgacga gggaaactgc tacgacaagc gcatcgacct gacgaacctg
      301 aagaagtcct acggcgccgg catcaggtgg gtgacaccca tggggccgct caggctggag
      361 tacgcgaggg tcatcaaccc tgaggactgg gaagagcctt cacgatggga gttctcgatc
      421 gggagcttct tctagccctc acgcaaagaa cgaactttaa agggagaacc tatgaaacga
      481 cacgtcacgt gggtatgcat cctcttaacg gtcctcttcg tgtcctgcct cccggccggg
      541 tcgcgggcgg aggaactcaa gatcgcctgt atcgactcca ggcgcgtcat caccgagtcc
      601 atggtgggca aggacgccta cgagaagctg aagaagctct ccgagcagaa ggaatcggag
      661 ctgaagaaac aggaggcgaa gatcaagagc ctgtcggacg agatccaggc caagtccgcc
      721 accatgtcgg catcggccaa ggaggatctc cagcagcgct acgagaagga agtcaaggaa
      781 tacaaccgtt cctacaagga cgcccaggac gacctgagga acacggagat gaacctcctc
      841 aagccgtgga cgaaggaact ggaggcgatc atcaaggact acggcgagaa gaacaagatc
      901 gacctcatcc tcgacaagac caacccggcg gtcatctacg gctcgaagaa ggtcgacatc
      961 accgacgcca tcatctccct cttcgacaag cgctatcagg agaagtcctc ccagcagaag
     1021 cagaagaaag agtagccatg aggttgtcca ggatcacgga gctcctgggc gggtcgctcg
     1081 cgggggagga ccgggagatc accggcgcat ccaccctgga ggaggcgacc ccgtccgaca
     1141 tatccttctt cgccaacaga aagtaccgcg aggccgcctt aaggacgcgt gcgggcgcct
     1201 tggtgacgtc cgaacccctg gacgccccgt gctcgcagat cgtcacggat aacccgtacc
     1261 tctacttcgc ccgcgtcctg ggtctcctcc accccgagcc gcagaggccc cgcggcg
//
LOCUS       DAOSCR010000709         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_69254_length_1313_cov_2.865660, whole genome shotgun sequence.
ACCESSION   DAOSCR010000709 DAOSCR010000000
VERSION     DAOSCR010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69254_length_1313_cov_2.865660"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLT69_12975"
     CDS             complement(<1..688)
                     /locus_tag="PLT69_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ15400.1"
                     /translation="MIGAITGDVIGSVYEWRRIKTKDFPLFTPKSRFTDDTVLTVALA
                     EAILEGADYPDLMRRYYHRHPNAGYGGMFRRWALDPQAAPYHSFGNGAAMRIAPAAYA
                     SRTLEEALEKAAHFTSCTHDHPEGIKGAQATAAAVFMARSGASRESIKSFVEDTFGYD
                     LSRTLEEIRPHYRFDPTCQGSVPEAITAFLESTSYEDAVRNAVSLGGDSDTLACMTGA
                     MAEAFYGGVPE"
     gene            complement(685..1071)
                     /locus_tag="PLT69_12980"
     CDS             complement(685..1071)
                     /locus_tag="PLT69_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ15401.1"
                     /translation="MGDPLGAREHLDLGLSYELTGDLAGARREYLRAASLDPSWATPW
                     FNLGNLSYASGDLASAEKHYEEALRRNADDPDVLNNLALTKHGLGKDEEALALVRRAL
                     SMRECPAYLDTLGTIERAMKPEGGTP"
     gene            complement(1074..>1313)
                     /locus_tag="PLT69_12985"
     CDS             complement(1074..>1313)
                     /locus_tag="PLT69_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15402.1"
                     /translation="RPVVALVDLGFWVFSRPHYVVVTGWGPGGVRAHTGTEADKVFPK
                     EEFERIWRKRGSVYLLVLPPSPWPGPAAPAPPSGS"
BASE COUNT          188 a          447 c          441 g          237 t
ORIGIN      
        1 cctccggaac gccgccgtag aacgcctccg ccatggcacc cgtcatgcag gccagcgtgt
       61 cgctgtcccc tcccagggag accgcgttcc gcacggcgtc ctcgtagctc gtcgactcga
      121 ggaacgccgt gatcgcctcg gggacgctgc cctggcaggt ggggtcgaac cggtagtgcg
      181 gcctaatctc ctccagcgtc cgcgacaggt cgtacccgaa cgtgtcctcc acgaacgact
      241 tgatggactc cctcgacgcg ccgcttctgg ccatgaacac tgctgccgcg gtggcctgcg
      301 cgccttttat cccctcgggg tggtcgtgcg tgcacgaggt gaagtgcgcg gccttctcga
      361 gcgcttcttc cagcgtgcgg gaggcgtagg ctgcaggggc gatcctcatg gcggcgccgt
      421 ttccgaaact gtggtagggc gcggcctggg gatcgagcgc ccaccgcctg aacatgccgc
      481 cgtaccccgc gttcgggtgg cggtggtaat accgccgcat gaggtcgggg tagtcggcgc
      541 cctccaggat cgcctcggcg agcgcaaccg tgagcaccgt gtcgtctgtg aagcggctct
      601 tgggggtgaa cagggggaaa tccttggtct tgatgcgtct ccactcgtac accgaaccga
      661 tgacgtcccc ggttatcgcg ccgatcacgg cgtgcctcct tcgggtttca tcgcccgctc
      721 gatggtgccg agggtatcca ggtatgcggg gcattccctc atggacagcg ccctgcgcac
      781 cagcgccagg gcctcttcgt cttttcccag gccgtgcttg gtgagcgcca ggttgttgag
      841 aacgtccggg tcgtccgcgt tcctcctgag cgcctcttcg tagtgcttct ctgcggaggc
      901 gaggtcgccg ctcgcgtagg agaggttgcc gaggttgaac cagggcgtcg cccaggatgg
      961 atcgagcgat gcggccctca gatactcccg ccgggccccg gcgaggtcgc ctgtgagctc
     1021 gtagctcagg cccaggtcga ggtgctcgcg ggcgcccagt gggtcgccca tggtcacgat
     1081 cccgagggcg gtgcaggcgc agcaggcccc ggccagggcg agggcggcag cacgagcaag
     1141 tatacggagc cccttttccg ccatatcctc tcgaactcct ctttcgggaa caccttgtcg
     1201 gcctcggtcc ccgtatgggc acggacgccg cctgggcccc acccggtgac caccacgtag
     1261 tgcggcctgg agaacaccca gaagccgagg tccacgagcg cgacgaccgg cct
//
LOCUS       DAOSCR010000710         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_69260_length_1313_cov_2.754372, whole genome shotgun sequence.
ACCESSION   DAOSCR010000710 DAOSCR010000000
VERSION     DAOSCR010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69260_length_1313_cov_2.754372"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLT69_12990"
     CDS             complement(<1..1097)
                     /locus_tag="PLT69_12990"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOJ15403.1"
                     /translation="MRIAVLTGGGDCPGLNGAIKWVTKTALDPVLEKKRGFKCEVVGI
                     REGWKGLVYVDPDDPESVARHTMHLDEEVVRTWDRYGGTNLGTSRTNPFNPQKDRSDQ
                     VIENIRKLGIDVIVAIGGEDTLGVAYKLHRRGVRTVGIPKTIDGDLVGTEYSLGFDSA
                     VNVIMEEIDRLRTTAGSHNRIFVVETMGRHAGWLALHGGECSGAYIILIPEVPFTMER
                     ICELLQERRGREVQYSIIVVSEGAKVGGTQEFYKDDRVDEFGHRSLGGIAAYISDEIQ
                     KRTGLESRYVNLSHLQRGGSPSARDRLMARWFGIAAVDMIVNEDFGRMVSFLHGEITT
                     VPMKEVIDRLRLVDVDKYYDVERYNGKRTIRA"
BASE COUNT          213 a          445 c          379 g          276 t
ORIGIN      
        1 gcgcggatgg tgcgcttgcc gttgtagcgc tccacgtcgt agtacttgtc cacgtcgacg
       61 agcctgaggc ggtctatgac ctccttcatg ggcaccgtgg tgatctcgcc gtggaggaag
      121 ctcaccatcc tgccgaagtc ctcgttcacg atcatgtcga cagcggcgat cccgaaccag
      181 cgtgccatga gcctgtccct cgccgaaggc gaaccgcccc tctgcaggtg gctcaggttc
      241 acgtacctag actcgagacc ggtcctcttc tggatctcgt cggagatgta ggccgcgatc
      301 ccccccaagg agcggtggcc gaactcgtcc accctgtcgt ccttgtagaa ctcctgggtt
      361 ccacccacct tggccccctc cgagaccacg atgatgctgt actggacctc cctgcccctc
      421 cgttcctgca ggagctcgca tatgcgttcc atggtgaagg gcacctcagg gatgaggatg
      481 atgtatgcgc cgctgcactc cccgccgtga agcgcgagcc accccgcatg cctgcccatg
      541 gtctccacca cgaagatcct gttgtgggag ccggccgttg tcctgagcct gtctatctcc
      601 tccatgatca cgttcaccgc cgagtcgaac ccgagggagt actcggtccc cacgaggtcg
      661 ccgtcgatgg tcttgggtat accgaccgtc ctgacgccgc ggcggtggag cttgtaggcc
      721 acgccgaggg tgtcctcgcc accgatggcg acgatcacgt ctatgccgag cttcctgatg
      781 ttctcgatga cctggtccga gcggtccttc tgggggttga acgggttggt cctcgacgtg
      841 cccaggttgg tgccgccgta acggtcccag gtgcggacga cctcctcgtc gaggtgcatg
      901 gtgtgcctgg caacgctctc cgggtcgtcg gggtctacgt acacgagacc cttccaccct
      961 tccctgatgc ccaccacctc gcacttgaac cccctcttct tctcgagcac agggtcaagc
     1021 gcggtcttcg tcacccactt gatcgccccg ttcagcccgg ggcagtctcc gcctcccgtc
     1081 aaaaccgcta tcctcatggt aaggttccct ccgatcggag tgatgtgtcg gcatccaggc
     1141 ttcacagcct cccgtgcgac cattataatc tcactgaaca tctcggcaag tcgcttgtgg
     1201 aaattgagcg ccgggcgcgg gcggtccagg tctggacgac atcagcccgc gcacagggac
     1261 ggggggatct catgaaggtg gtgttccacg aggatttcta ccggcactac aca
//
LOCUS       DAOSCR010000711         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_69498_length_1310_cov_2.994422, whole genome shotgun sequence.
ACCESSION   DAOSCR010000711 DAOSCR010000000
VERSION     DAOSCR010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69498_length_1310_cov_2.994422"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLT69_12995"
     CDS             complement(<1..365)
                     /locus_tag="PLT69_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOJ15404.1"
                     /translation="MGDGLTFRVLAYAVMVAHAAFVLFVVAGGVAVRWSPRLAWIHLP
                     CVFWGIVVEAFGLTCPLTPLENEFRARAGIDPYAGDILIHLMDSMIYPGWLTRDIQVV
                     LAVIVAAVNVAVYGSLLVR"
BASE COUNT          268 a          441 c          350 g          251 t
ORIGIN      
        1 ttcctcacca ggagagaccc gtagaccgcc acgttcaccg ccgcgacgat cacggcgagc
       61 acgacctgga tatccctcgt gagccacccg gggtatatca tggaatccat gaggtggatc
      121 aggatgtcgc ccgcgtacgg gtcgatcccc gccctcgccc tgaactcgtt ctcgagcggg
      181 gtgaggggac acgtgagccc gaaggcctcc accacgatcc cccagaacac gcacgggagg
      241 tggatccagg ccagcctggg cgaccacctg acggccacgc ccccggccac gacaaagagg
      301 acgaacgccg cgtgagcgac catcaccgcg tatgcgagga cccggaaagt gagcccatct
      361 cccataccat cgaaaggtaa tgactgaggc ccgggcgtac aagcgtattg gttgtctttt
      421 tagcgttccg gcgggaggcc catcggggcc gcgccatcaa tcggcgtatc ccccctgaag
      481 gcggccacgg gagaaaccct ttcactcttt tcacatcctt cattcaggtc catccaggtc
      541 tgccgtcaac gacacaggcc caacgccacg aacctcgccg ccttcgccag gaccatcacc
      601 cgttcccctg gagggctgcc ccgggacacg ccctcgagcc tggcagtgcg cctctctgca
      661 ggccttacgg gtttcatgcc ctgtcgatgc cggcaaccaa acacctctct tccccgggtc
      721 gtcgtgcttt tccctcaggt ccccacaacg attattgcgc gcacgccatg gccttttgcc
      781 ctttgctgat cggccatgat catgggcatg tccatgatcg tccaggggct tgccataaga
      841 gaatcacgaa gaatagtcca ttttcgcgag ctatcgagac atcttttcgc cgcttccgtg
      901 atatcgggat gttacaaggg cgaccggtcc ccgcgatgtt tctatcccct tgttttccat
      961 tgcgatttct cgaaaccttg actgccgtgg cctatcctgg tagtgaacac acgtacacac
     1021 aacgacatcg gggccatgca tcctggccga atcaatgacc gacagcgctg agaacgagga
     1081 ggtatgtcat gtcatcgaga tatgctctcg ctccagttgt ctgcctgaca gtcttgatgt
     1141 tcgtcgctgc atccagtcat cagctgtatg cgcgtccctt gccggaaaag acgaacccgt
     1201 ccgagctcgc ccgcatccac agcacgggca aaccggaggc ccggtcacac gcggccgggc
     1261 atgagcaaag ggatgccaag actgtttccc cgacaaaggc acaaccccaa
//
LOCUS       DAOSCR010000712         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_69956_length_1304_cov_3.056845, whole genome shotgun sequence.
ACCESSION   DAOSCR010000712 DAOSCR010000000
VERSION     DAOSCR010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69956_length_1304_cov_3.056845"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PLT69_13000"
     CDS             complement(<1..1231)
                     /locus_tag="PLT69_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOJ15405.1"
                     /translation="MDELVKIFLEESEDEIRELETGLMRLEEDSGDEDTINRVFRAAH
                     TIKGSAGLVGFEEVSNYTHTIESILDRIRKKELTVTKALVSSLLGGVDFLKRMISAEV
                     EGSPVDSNEVEEALKHIKRFLGMKGVSRDAQGKAVEATGKTPEKVYLITMKFRPDILS
                     TGQDPLMLIRELMDMGEIIESRAFTEDIPGFYELQPTTCYTSWEIVLKTSRPISEINN
                     VFIFVMDENEISIHDISSQYKNGVDLSLAEKPIGEILVEKGIVRETDVIEALREHKTT
                     GQALVEKGKVSRETVEKMALAQSESRKVAKATTIRVDTDKLDRLMNLVGEMVISVARV
                     SQCANEITDASVSKSLTAATASLERISRDLQEQVMRVRMVPVEGTFNRFKRVVRDMSF
                     ELGKKIEIKMSGTETELD"
BASE COUNT          212 a          407 c          365 g          320 t
ORIGIN      
        1 tgtcgagctc ggtctccgtg cccgacatct tgatctcgat cttcttgccg agctcgaagg
       61 acatgtccct gaccaccctc ttgaacctgt tgaacgtccc ctcgaccggc accatgcgaa
      121 ccctcatgac ctgttcctgg agatccctgg agatgcgctc cagcgatgcg gtcgcggcgg
      181 tgagggattt cgacaccgag gcgtcggtta tctcgttggc gcactgggag acgcgggcca
      241 cggagatcac catctcgccg accaggttca tgagcctgtc gagcttgtcc gtgtccaccc
      301 tgatggtcgt cgccttcgcc actttccggc tttccgactg ggcgagggcc atcttctcca
      361 ctgtttccct cgaaaccttg cccttctcga caagtgcctg gcccgtggtc ttgtgttccc
      421 tcagtgcctc gatcacgtcc gtctccctca cgatcccttt ctcgaccagg atctcgccga
      481 tgggtttctc ggccagggag aggtccaccc cgttcttgta ctgggaggag atgtcgtgga
      541 tcgatatctc gttttcgtcc atgacgaaga tgaagacgtt gttgatctcg gagatcggcc
      601 tggatgtctt cagcacgatc tcccaggacg tgtagcacgt ggtcggctgg agctcgtaga
      661 aaccggggat gtcctcggtg aaggcccggg actcgatgat ctcgcccatg tccatgagtt
      721 ccctgatgag catgaggggg tcttgcccgg tggacaggat gtcaggcctg aacttcatgg
      781 tgatcaggta taccttttca ggcgtcttcc cggtcgcctc gaccgccttg ccttgcgcat
      841 ccctggaaac acccttcatg ccgaggaagc gcttgatgtg cttgagggcc tcttccacct
      901 cgttcgagtc gacgggcgag ccctcgacct cggccgagat catcctcttc aagaagtcca
      961 ccccgccgag gagcgaggag acgagggcct tggtgaccgt cagttccttc ttccgtatcc
     1021 tgtcgaggat gctctcgatg gtatgggtgt agttgctcac ttcctcgaaa ccgacaagac
     1081 cggcagagcc cttgatcgtg tgggccgcgc ggaagacccg gtttatggtg tcctcgtcgc
     1141 cgctatcctc ctccagcctc atgagcccgg tctcgagctc cctgatctcg tcctcgctct
     1201 cttcgaggaa tatcttcacg agttcatcca tggtcttgac ctacctcatg aatttcttgg
     1261 tcacgtatac gagctggtcc ggcttgaagg gcttgacgag ccag
//
LOCUS       DAOSCR010000713         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_70184_length_1301_cov_3.745586, whole genome shotgun sequence.
ACCESSION   DAOSCR010000713 DAOSCR010000000
VERSION     DAOSCR010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70184_length_1301_cov_3.745586"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLT69_13005"
     CDS             <1..249
                     /locus_tag="PLT69_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ15406.1"
                     /translation="FIALIGGRGTIAGPVIGALLLRPVSEFTRIYLSSVLPGLHLVIF
                     GAILILVMIYQPFGLVRPLSRFYDRMLDALSGEKKKER"
     gene            257..982
                     /locus_tag="PLT69_13010"
     CDS             257..982
                     /locus_tag="PLT69_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ15407.1"
                     /translation="MALIEIKDISKHFGGLKAVDQVSISIEKGQIYGLIGPNGAGKST
                     LFNCVAGLYPPTSGKVIYDGEDITGWKPWNVCRKGLARTFQIVKPFQKRTVLYNVMVG
                     SFLKTSNPSEARDRAMEVLKRFNLADKWNILAGNLTIADRKRLEIARAMATEPDVLLL
                     DESMAGLRPNEVDETLDEIRKIRDSGVTIFMIEHIMRAIMTVSDRITVIQFGKKIMDG
                     TPQEVANDERVIKAYLGESYGAA"
     gene            969..>1301
                     /locus_tag="PLT69_13015"
     CDS             969..>1301
                     /locus_tag="PLT69_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ15408.1"
                     /translation="MALLEIRNIDCFYGDVQVIFGVSMHVDEGEVISLIGANGAGKST
                     LLRTVSGLMKPRSGEILFENEPVHDLRPEQIVERGIIQVPEGRRLFSLMTVRENLEVG
                     AYNSRAYPH"
BASE COUNT          289 a          390 c          401 g          221 t
ORIGIN      
        1 ttcatcgccc tcataggcgg acggggcacc atagctgggc cggtcatcgg ggcccttctc
       61 ctgaggcctg tgagcgagtt cacgaggata tacctgagct ccgtgctgcc tggactgcac
      121 ctggtgatct tcggggccat cctcatcctg gtcatgatct accagccctt cggactggta
      181 aggcccctgt cacggttcta cgacaggatg ctcgacgcgt tgagcggcga gaagaagaaa
      241 gagaggtgat gctcccatgg cgctcatcga gatcaaggac atatccaagc acttcggcgg
      301 gctcaaggcg gttgaccagg tgagcatatc catcgagaag ggccagatct acggcctgat
      361 aggccccaac ggcgcgggga agtcgacctt gttcaactgc gttgccggcc tgtacccccc
      421 cacctcgggc aaggtcatct acgacggcga ggacatcacg gggtggaagc catggaacgt
      481 ctgcaggaag gggctcgcgc gcaccttcca gatcgtcaag ccgttccaga agaggacggt
      541 cctctacaac gtcatggtgg gttctttcct caagacctcg aacccgtcag aagcccggga
      601 cagggccatg gaggtcctca agcgcttcaa cctcgccgac aagtggaaca tcctggcggg
      661 aaacctcacc atagccgacc gcaagcgact cgagatagcc agggccatgg cgaccgagcc
      721 ggacgtcctc ctcctcgacg agagcatggc cggcctcagg ccgaacgagg tcgacgagac
      781 cctcgacgag atcaggaaga tacgggacag cggcgtgacc atcttcatga tcgagcacat
      841 catgagggcc atcatgaccg tctccgacag gatcacggtg atccagttcg gcaagaagat
      901 catggacggg acgccgcagg aggtggcgaa cgacgagcgg gtcatcaaag catacctggg
      961 ggagagctat ggcgctgctt gagatcagga acatcgactg cttctacggt gacgtgcagg
     1021 tcatcttcgg cgtttccatg cacgtcgacg agggcgaggt catcagcctc atcggcgcca
     1081 acggcgccgg aaagtccacc ctgctcagga cggtctccgg cctcatgaaa ccgagatcag
     1141 gcgagattct cttcgagaac gagccggtgc acgacttgag gcccgagcag atcgtggaac
     1201 gggggatcat ccaggtaccc gaaggcagga gactcttcag cctcatgacc gtgcgagaga
     1261 acctcgaggt cggggcatac aactccaggg cctatcctca c
//
LOCUS       DAOSCR010000714         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_70250_length_1301_cov_1.810594, whole genome shotgun sequence.
ACCESSION   DAOSCR010000714 DAOSCR010000000
VERSION     DAOSCR010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70250_length_1301_cov_1.810594"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLT69_13020"
     CDS             complement(<1..688)
                     /locus_tag="PLT69_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ15409.1"
                     /translation="MEPMGTLRPNLLSPRDLPWRIVWDRDRCTLCGRCTAACPTRSIE
                     LGVHRKRSLEVKAGLAVKPSNVYAVYHGIDQVAHASRVCVGCGLCSQVCPTAAIFPVR
                     SEEIDKVRFHVSEGGEPRRRGGRRNDPNSLLDRIKFIRISMLTDPALDAGRHEFELRT
                     LLGRIPPPEDMLEEASRPDGWVPPVREVYPLMVGGMSFGALSPTMWEGLQVGVAYLNE
                     ELGMPVRICTG"
     gene            complement(652..>1301)
                     /locus_tag="PLT69_13025"
     CDS             complement(652..>1301)
                     /locus_tag="PLT69_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOJ15410.1"
                     /translation="GYLGLDRQALSARIILAQGRQNTNYAITLYACHPFFIQGYATMT
                     NGENTAFVPIREYLVSRGFPGYTGYQSDSEVFTHILHYTHRRLGLPIQAYKHVLTPLK
                     DEELARHQDARFLRAVKHSLRFLTIDGPNCVIGCLPDKTLFMLQDSKKLRPGVAGGRP
                     GLFAFSSEMCGLDAAIPDRDTKNDRQPMKYDLVSVDASRTEIRTWNQWEPFGRTC"
BASE COUNT          206 a          421 c          427 g          247 t
ORIGIN      
        1 cgcccgtgca tatcctcacg ggcatcccga gctcctcgtt caggtacgcc acccccacct
       61 ggagcccctc ccacatggtg ggagacaagg ccccgaacga catgccgccg accatgagcg
      121 ggtagacctc gcgaacgggg ggcacccacc cgtccggcct cgaggcctct tcgagcatgt
      181 cctcgggggg cgggatcctc ccgaggagcg tcctcagctc gaactcgtgg cggcctgcgt
      241 ccagcgctgg gtcggtgagc atcgatatcc gtatgaactt gatgcggtcg agcaggctgt
      301 tcgggtcgtt cctcctgccc cccctcctcc tcggctcacc cccttcagag acatggaacc
      361 tcaccttgtc gatctcttcg ctcctcaccg ggaagatcgc tgcagtgggg cacacctgcg
      421 agcacagccc gcagcccacg cagaccctcg acgcgtgggc cacctggtct atgccgtggt
      481 agacggcgta cacgttcgaa ggcttgactg caaggccggc cttcacctcc agcgaccgct
      541 tcctgtggac gccgagctcg atcgagcgtg tcgggcaggc ggcggtgcac ctgccgcaca
      601 gggtgcacct gtccctgtcc cagacgatgc gccaggggag gtctctcggg ctcagcaggt
      661 tcggccgaag ggttcccatt ggttccatgt gcgtatctcc gttctcgatg cgtcgacgct
      721 cacgaggtcg tacttcatgg gctgacggtc gttcttcgtg tccctgtccg ggatcgcggc
      781 atcgagcccg cacatctcgg aagagaaggc gaagagcccc gggcggccgc cggcgacacc
      841 gggcctgagc ttcttgctgt cctggagcat gaacagcgtc ttgtcaggga ggcacccgat
      901 gacgcagttc ggcccgtcga tggtgaggaa cctgagcgaa tgcttgacgg ccctgaggaa
      961 cctggcgtcc tggtggcggg caagctcctc gtccttcagc ggggtgagga cgtgcttgta
     1021 cgcctggatc ggcaggccca gcctgcggtg cgtgtagtgc aggatgtggg tgaacacctc
     1081 ggagtcggac tggtaacccg tgtatcccgg gaaccctctc gacacgaggt attctcgtat
     1141 gggcacgaac gccgtgttct cgccgttggt catggtcgcg tacccctgga tgaagaaggg
     1201 gtggcacgcg tacagggtga tggcatagtt ggtgttctgg cgaccctgcg cgaggatgat
     1261 cctggcgctt aaggcctggc ggtcgagtcc aaggtagccg g
//
LOCUS       DAOSCR010000715         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_70291_length_1300_cov_3.012048, whole genome shotgun sequence.
ACCESSION   DAOSCR010000715 DAOSCR010000000
VERSION     DAOSCR010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70291_length_1300_cov_3.012048"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PLT69_13030"
     CDS             <1..1228
                     /locus_tag="PLT69_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ15411.1"
                     /translation="AGCLSVEATDPLYILYTSGTTGRPKGVMRDNGGHAVALAWSMEN
                     IYNVKPGDVYWAASDVGWVVGHSYIVYGPLIHGCTTVLYEGKPVGTPDPGAFWRVISE
                     HRVKVLFTAPTAFRAIKKEDPSGSYVKKYDISCLKHLFLAGERLDPDTYHWASDLLKI
                     PVIDHWWQTETGWAIAANCMGIEQLPVKAGSPTKPVPGYDVRIIDEAAREVGPGTEGV
                     IGIRLPLPPGCLPTLWENDEGYVSSYLTEYPGYYATGDGGYMDPDGYIYIMGRVDDVI
                     NVAGHRLSTGGMEEVVARHKDVAECAVIGVEDGLKGQVPIGFVVLKAGVDRPHEEITR
                     EIVDMVRNEIGALACLKDIAVVTRLPKTRSGKILRSTMRKIADGKEYTTPSTIEDPAA
                     LQEIAGAARTIGYPKQ"
BASE COUNT          251 a          413 c          449 g          187 t
ORIGIN      
        1 ggctggatgc ctctccgtcg aggccacgga ccccctctac atcctctaca cctcgggcac
       61 gacggggaga cccaaggggg tcatgcggga caacgggggc catgcggtgg cgctcgcctg
      121 gagcatggag aacatctaca acgtcaagcc cggcgacgtc tactgggccg cgtccgacgt
      181 gggttgggtc gtggggcact cgtacatcgt gtacggaccg ctgatccacg gctgcaccac
      241 cgtgctctac gagggcaagc ccgtgggaac cccagaccct ggtgcgttct ggagggtgat
      301 cagcgagcac agggtcaagg tgctcttcac cgcgccgacg gcattcaggg ccatcaagaa
      361 ggaggacccc tcaggatcct atgtcaagaa gtacgacatc tcgtgcctga agcacctctt
      421 cctcgccgga gagcggcttg accccgacac ctaccactgg gcgagcgacc tcctcaagat
      481 cccggtcatc gaccactggt ggcagacgga aaccgggtgg gccatcgcgg ccaactgcat
      541 ggggatcgag cagctgcccg tgaaggctgg ttcgccgacg aaaccggtcc cgggatacga
      601 cgtgcgcatc atcgacgaag cggcgcgcga ggtcggccca gggaccgagg gcgtgatagg
      661 catcaggctg cccctgccgc cggggtgcct gcccaccctc tgggagaacg acgagggtta
      721 cgtgtcgtcg tacctcaccg agtaccccgg ctactacgcc accggcgacg gcgggtacat
      781 ggatcccgac gggtacatct acatcatggg cagagtggac gacgtgatca acgtggcggg
      841 gcacaggctg tcgaccggcg gcatggaaga ggtcgtggcc aggcacaagg acgtggcgga
      901 gtgcgcggtc atcggcgtcg aggacggcct gaaggggcag gtgcccatcg ggttcgtcgt
      961 cctcaaggcc ggcgtggaca ggccgcacga ggagatcacc agggagatcg tcgacatggt
     1021 gcgcaacgag atcggcgccc ttgcctgcct gaaggatatc gcggtggtga ccaggctgcc
     1081 caagacgcgc agcggcaaga tcctccgatc gaccatgcgc aagatagccg acggaaagga
     1141 atacacgacg ccctccacca tcgaggaccc cgcggccctg caggagatcg ccggcgcggc
     1201 caggaccatc gggtatccga agcagtgaga gcatgagtcg ccggggggcc tgaccgcggc
     1261 cccccgggtt cgtcctcgag ggccgtatga ccgggccgtg
//
LOCUS       DAOSCR010000716         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_70623_length_1296_cov_2.574537, whole genome shotgun sequence.
ACCESSION   DAOSCR010000716 DAOSCR010000000
VERSION     DAOSCR010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70623_length_1296_cov_2.574537"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1296)
                     /gene="tssI"
                     /locus_tag="PLT69_13035"
     CDS             complement(<1..>1296)
                     /gene="tssI"
                     /locus_tag="PLT69_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HOJ15412.1"
                     /translation="LVSEDTSIDPRALIGKGATVFVRLAQDETRPINGVISRFRQERL
                     GVPEGPERLMHMATYTAVLVPWTWLLSKSMNSRIFQKKDVVQIVEQVFGEWKFSHYEF
                     RLTGSYAKREYCVQYHESDLDFVSRLLEEEGIFYFFEHERSRHVLVMADSPDAHRDCP
                     VQSTVRCLLDKGAEAVQAEDVITHLGWGEEIRIASYVTTDYNYLMPSTDLLVEMPTSI
                     EVGTGKREVYDFPGRYGTRADGERYAELRMQAEETQVTKLHGVGNCRTFSSGYRFTLE
                     DYHRKEMNGRPYVLVDVEHEAREPVELTGSAPEASYTNTFTCIPHGVAFRPLRLTPKP
                     VVQGVQTATVTGPPGEEIYTDEHGRVKVQFPWDREGLRNEETTCFIRVGQVWAGQGWG
                     AGLWESANIRVHLTGQVVVTTGSLPHGQGVETTFAQIVA"
BASE COUNT          195 a          448 c          400 g          253 t
ORIGIN      
        1 cggcgacgat ctgcgcgaag gtcgtctcga cgccctggcc gtgcggcagc gagccggtgg
       61 tcaccaccac ctggccggtc aggtgcacgc ggatgttcgc gctttcccac aggccggcgc
      121 cccagccctg gccggcccag acctggccca cgcggatgaa gcatgtggtc tcctcgttcc
      181 tgaggccctc gcggtcccag gggaactgga ccttcaccct cccgtgctcg tcggtgtaga
      241 tctcctcgcc cggaggcccg gtgaccgtgg ccgtctgcac gccttgaacc acgggtttcg
      301 gggtgagccg aaggggcctg aatgcgactc cgtggggaat gcacgtgaag gtgttcgtgt
      361 acgacgcctc gggtgcggac cccgtgagct ccacgggttc cctcgcctcg tgctccacat
      421 ccacgaggac gtacggcctg ccgttcatct ccttgcggtg gtagtcctcg agcgtgaacc
      481 tgtatccgct cgagaacgtc ctgcagttgc ccacaccgtg gagtttcgtg acctgggtct
      541 cctcggcctg catgcggagc tctgcgtagc gctcgccgtc cgccctcgtc ccgtaccgcc
      601 cggggaaatc gtacacctcc cgcttgcccg tccccacctc gatgctcgtc ggcatctcca
      661 ccagcaaatc ggtgctcggc atgaggtagt tgtagtcggt ggtcacgtag gaggcgatcc
      721 tgatctcctc gccccagccc aggtgcgtga tcacgtcctc ggcctgcacc gcttctgccc
      781 ccttgtcgag caggcatctc accgtggact ggaccgggca gtccctgtgg gcgtccggcg
      841 agtcggccat gacgagcacg tgccgcgagc gctcgtgctc gaagaagtag aagataccct
      901 cttcctcgag gagcctgctc acgaagtcga ggtcgctctc gtggtactgc acgcagtact
      961 ccctcttcgc atacgaaccg gtcagcctga actcgtagtg ggagaacttc cactcaccga
     1021 agacctgctc cacgatctgg accacgtcct tcttctggaa gatcctcgag ttcatggatt
     1081 tcgagaggag ccacgtccag gggaccaaga ccgccgtgta ggtggccatg tgcatgagcc
     1141 gctccgggcc ctcggggacg cccaggcgtt cctgcctgaa cctcgatatg acgccgttga
     1201 tcggcctcgt ctcgtcctgg gcgagcctca cgaacacggt cgcgcccttt ccgatgagcg
     1261 ccctcgggtc gatggacgtg tcctcggaga cgaggg
//
LOCUS       DAOSCR010000717         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_71017_length_1291_cov_2.978964, whole genome shotgun sequence.
ACCESSION   DAOSCR010000717 DAOSCR010000000
VERSION     DAOSCR010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71017_length_1291_cov_2.978964"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLT69_13040"
     CDS             complement(<1..661)
                     /locus_tag="PLT69_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ15413.1"
                     /translation="MPPDAALTHELCTELCRISFEAGVQVGVILNRLGGVEYVMAGTA
                     TGISIPPLERMRTHPGRLQGLRLIHTHLDGSPLSFEDLTDLAKLRLDLVYAVEVRPDG
                     GPGLAYGAHLLPRGEDGRLWKLMEPVPTHRVGMPFDEFISSLEDEINRTQDTLVKGAH
                     EDRAILVVVSAAPADELQDMIHEFRALAEAASITVVDVVVQRRPKPDPRYVIGKGKVN
                     EL"
     gene            complement(762..>1291)
                     /locus_tag="PLT69_13045"
     CDS             complement(762..>1291)
                     /locus_tag="PLT69_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOJ15414.1"
                     /translation="SYRVPVEHEMKQWFVVQTNPKEDEVACLVLRQQGVEIYQPFMEK
                     YVFHARRKVLKKYPLFPNYLFVQIAPTEEDYHKVRWCRGVRRILQDNYQPVPIEDEFI
                     RSLSSLEDKESGIIKKPVSFAPGDLVRIKSGPLKDLYGVFEAWGSDEGRVRILIEMVN
                     NRAKVLVHPSIIEKA"
BASE COUNT          198 a          422 c          417 g          254 t
ORIGIN      
        1 acagctcgtt caccttgccc ttgccgatga cgtagcgggg gtccggcttc ggtctcctct
       61 ggaccaccac gtccacgacc gttatggacg cggcctcagc cagggccctg aactcgtgga
      121 tcatgtcctg gagctcgtct gcgggcgccg cggagacgac cacgaggatc gcgcggtcct
      181 cgtgcgcgcc cttgaccagg gtgtcctgcg tccggttgat ctcgtcctcg agcgaggaga
      241 tgaactcgtc gaagggcatg cccacgcgat gggtggggac gggttccatg agcttccaga
      301 ggcgcccgtc ctccccgcgg ggcaggaggt gggcgccgta ggcgagtccg gggccgccgt
      361 cgggcctcac ctcgacggcg tagacgaggt cgagcctgag cttcgccagg tccgtcaggt
      421 cctcgaagga gagcggcgac ccgtccaggt gggtgtggat gagcctcagg ccctgcagcc
      481 ttcccgggtg ggtcctcatg cgctcgaggg gggggatcga tatgccggtg gcggtgccgg
      541 ccatgacgta ctccacgccg cccaggcggt tgaggatgac gccgacctgg acgccggcct
      601 cgaacgagat gcggcagagc tcggtgcaca gctcgtgggt cagggcggcg tccgggggca
      661 cccgcctccg gtagagtctc tccagggccc tttcctgcga ggccttcagc gtcttgctgg
      721 tcccgaagat cttcctgata agccggcctc cccgcctgct ctcaggcctt ctcgatgatc
      781 gacgggtgca cgagcacctt cgcgcggttg ttcaccatct cgatgaggat ccgcaccctt
      841 ccctcgtccg acccccaggc ctcgaagacc ccgtagaggt ccttcaacgg cccggacttt
      901 atccgcacca ggtcaccggg agcaaaggac acgggcttct tgatgatgcc ggattccttg
      961 tcttcgaggc tgctcagcga acggatgaac tcgtcctcga tgggtacggg ctggtagttg
     1021 tcctggagga tcctcctgac ccccctgcac cacctgacct tgtggtagtc ctcctccgtg
     1081 ggagcgatct gcacgaagag gtagttgggg aacagggggt acttcttgag gaccttgcgc
     1141 ctggcgtgaa acacgtactt ctccatgaaa ggctggtata tctccacccc ctgctgcctg
     1201 aggaccaggc aggcgacctc gtcctccttg gggttggtct gtaccacgaa ccattgcttc
     1261 atttcgtgct ccaccggcac tctatagctc a
//
LOCUS       DAOSCR010000718         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_71419_length_1286_cov_3.165719, whole genome shotgun sequence.
ACCESSION   DAOSCR010000718 DAOSCR010000000
VERSION     DAOSCR010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71419_length_1286_cov_3.165719"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..521)
                     /locus_tag="PLT69_13050"
     tRNA            complement(447..521)
                     /locus_tag="PLT69_13050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Val,seq:gac)
     gene            863..>1286
                     /locus_tag="PLT69_13055"
     CDS             863..>1286
                     /locus_tag="PLT69_13055"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOJ15415.1"
                     /translation="MRIPPHSLAFDFDGVIADTFRLFVRMARQRYNYSFEYEDITDYE
                     FLKCIDMDRSHAFEIIEVLTNEPHELDLFPNVGAREVLGRVGSVSGVLVVTARPFAEP
                     VELWFRRHLPEIDSGCLKVEATGINTAKLEVLTRLGVTH"
BASE COUNT          243 a          431 c          381 g          231 t
ORIGIN      
        1 gcagcgtcgc tgaacaccgt cacgtgacca ttgagcggcg cgtcgccggt gttggcgatg
       61 gtgaccgagc cacccaggcc ctccccgccg accggcacgc ccgggcagac gtatacacct
      121 atcacggaaa acatgcacca cccgagcccg aacatctctt tttttaattc tttgggtccc
      181 cgtccggccc accaaccgca ctgggagccc acagcaccat ggccctcgcc gagaagatac
      241 cgcgcactct ccgattcacc ggctgagacg gatcgacgct ttccaacggg ttctcttttg
      301 gggaaaagac cgtcgacgcg tcttctgctg tgagcgcccg tatccgcgcc agagggatac
      361 acccacgaag gaacgaggaa ggcattcggt ggtctttgga tcgccgcagg atgaatgcac
      421 ctcaatgggt gcattgacga ggcaagtggt aggcacgcag ggatttgaac cctggacccc
      481 caccgcgtca aggtggtgct ctccccctga gctacgtgcc tatgcgacgc agttttcgtt
      541 agcccgtatg tccgttcctt gtcaaccggt ttttcgtgcc ccggcctgaa cctccagccg
      601 cgccatggcg acgtgtccct cgtatgcttc agcctgctcg atgccgaggc ccgcacgccc
      661 ctgccgaggc cccgagaagg gtcccgcgcc ttggaggtcg gcacctgcct tgaacgaaaa
      721 ccaggggccc ccggccgtcc cccgaaccct tccggcgaag agagcgttct tcatggagag
      781 ccggctctcg cacgacgccc gtttgcacgc gcgagcgatc tgtggtacac aggccggtac
      841 cgaaaaacca cggggacacc cgatgagaat accgccgcat tcactcgcct tcgacttcga
      901 cggggtcatc gccgacacct tcaggctctt cgtccgcatg gcccgccaga gatacaacta
      961 ctccttcgag tacgaggaca taaccgacta cgagttcctc aagtgcatcg acatggacag
     1021 gtcgcacgcc ttcgagatca tcgaggtgct gaccaacgag ccgcacgagc tggacctgtt
     1081 cccgaacgtg ggcgcgaggg aggtgctcgg acgggtgggc tcggtctcgg gcgtgctcgt
     1141 ggtgacggcg aggccctttg cggaaccggt cgagctctgg ttcaggcgcc acctgccgga
     1201 gatagacagt ggttgcctca aggtggaggc caccggcatc aacacggcca agctcgaggt
     1261 gctcacgagg ctcggcgtca cccatt
//
LOCUS       DAOSCR010000719         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_71508_length_1285_cov_3.477236, whole genome shotgun sequence.
ACCESSION   DAOSCR010000719 DAOSCR010000000
VERSION     DAOSCR010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71508_length_1285_cov_3.477236"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLT69_13060"
     CDS             complement(<1..942)
                     /locus_tag="PLT69_13060"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ15416.1"
                     /translation="MKSDASLTPMMRQYLEIKRECPDAVLFYRMGDFYEMFYDDAVEA
                     SRILGLALTTRDKGKEHPVPMCGVPFHSAQAYIHRLIKAGRKVAVCEQSEPEAGSRGL
                     MRREIVRVVTPAIALEDDHLERDRANYLMSLWRDPKSRTVGLAFIDVSTGAFRATELD
                     GEDRLLSEIARVAPSEVLIAQDFDLPEGLAVTRIDEPMTLERARDLLNQHFRTVTLDG
                     FGLKDAPLATVASGMALSYVRKTCGTAIEHITTLRFYSQGFSMALGRTTIRNLELFET
                     IGSSRDNTLYAVLNHTRTPMGARMLADWIAFPLVDVER"
     gene            complement(932..>1285)
                     /locus_tag="PLT69_13065"
     CDS             complement(932..>1285)
                     /locus_tag="PLT69_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15417.1"
                     /translation="ELESGVLIEVEDKTKHLSGDLYMVHMAVTFSGELGDKDLELLAY
                     CPSGRLAITRVLNRPGVHKDDLERVKTELVESFIATNKAYMVHPRFVERFKKTSLERF
                     KEQEEKARRMAGHEE"
BASE COUNT          207 a          413 c          399 g          266 t
ORIGIN      
        1 ccgctcgaca tccacgaggg ggaaggcgat ccagtccgcg agcatgcgcg cgcccatggg
       61 tgtcctcgtg tggttcagga cggcgtacag ggtgttgtca cgggaagagc ctatggtctc
      121 gaagagctcc aggttccgta tcgtggtccg gccgagtgcc atggagaagc cctggctgta
      181 gaacctcagg gtggtgatgt gctctatggc cgtcccgcac gtcttcctca cgtaggaaag
      241 ggccatcccc gaggccaccg tcgcaagcgg cgcatctttg aggccgaagc cgtccagggt
      301 gaccgtacgg aagtgctggt tcaggaggtc ccgggctctc tccagggtca tgggctcgtc
      361 gatcctggtc acggcgagcc cctcgggcag gtcgaagtcc tgtgctatca agacctcgct
      421 gggcgccacc ctcgcgatct ctgataaaag cctgtcctct ccgtcgagct ccgtcgcccg
      481 gaaggccccc gtggagacgt cgatgaaggc caggcccacg gttcggcttt tcgggtcacg
      541 ccagagggac atgaggtagt tcgccctgtc cctctcgagg tggtcgtcct cgagcgctat
      601 ggcaggggtc accacgcgga cgatctccct gcgcatgagc cccctcgacc ctgcctcggg
      661 ttcggactgc tcgcagaccg cgaccttccg gccggccttg atgagcctgt ggatgtacgc
      721 ctgggcgctg tggaacggca caccgcacat ggggacggga tgttccttcc ccttgtccct
      781 cgtcgtgagc gcgaggccca ggatcctcga ggcctccacg gcgtcgtcgt agaacatctc
      841 gtagaagtca cccatccggt agaacaggac cgcgtccggg cactccctct tgatctcgag
      901 atactggcgc atcatggggg tcagcgaggc gtcactcttc atgaccggcc atcctcctgg
      961 ccttctcctc ctgctccttg aatctctcga ggctcgtctt cttgaacctc tcgacgaagc
     1021 gggggtgaac catgtaggcc ttgttcgtgg cgatgaacga ttcgacgagt tcggtcttga
     1081 cccgctcgag gtcgtccttg tggacgcccg gcctgttgag aaccctcgtg atcgcgagcc
     1141 tgccggacgg gcagtacgcg agaagctcta agtctttgtc accgagttcc cccgagaagg
     1201 ttaccgccat gtgcaccatg tagagatcgc ccgagaggtg tttcgtcttg tcctcgacct
     1261 ctatgagcac cccgctttcg agctc
//
LOCUS       DAOSCR010000720         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_72290_length_1276_cov_3.157248, whole genome shotgun sequence.
ACCESSION   DAOSCR010000720 DAOSCR010000000
VERSION     DAOSCR010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72290_length_1276_cov_3.157248"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLT69_13070"
     CDS             complement(<1..469)
                     /locus_tag="PLT69_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ15418.1"
                     /translation="MEPVAIMPCLDMLEGRVVKGVHFVDIRDAGDPVEHARFYQDQGA
                     DELAMLDIAATQENRKTRLEWVKNVSSAIEIPLTVGGGISSLEDIEAVLEAGADKVSM
                     NSAAVRNPRLVSEASRAFTAGRITIAIDARRNPAMPSGFELVISGGRLPVGQDA"
     gene            complement(780..>1276)
                     /locus_tag="PLT69_13075"
     CDS             complement(780..>1276)
                     /locus_tag="PLT69_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ15419.1"
                     /translation="SANLAASCMVKGDPAQLEQVFTNLCDNAAHAMGKTGGEITVSLA
                     RATVPEGEREALGLPAGRCIVLRVSDTGPGIPGYALSRVFDPYFTTTWKGEGSGLGLS
                     IVHGIVKAHEGAVTCTSAEGQGACFTVYLPEYVDDKELGQGTKDSAWDSGRTDGLDPS
                     GAHR"
BASE COUNT          183 a          450 c          413 g          230 t
ORIGIN      
        1 ctgcgtcctg gccgaccggg agcctgccgc ccgagatcac gagctcgaag ccggacggca
       61 tcgccgggtt cctgcgcgca tcgatcgcga tggtgatcct ccccgcggtg aaggcccggg
      121 acgcctcgct gacgagccga gggttcctca cggctgcgga gttcatggag accttgtccg
      181 ccccggcctc gaggacggcc tcgatatcct cgagcgatga gatgccgccg cccacggtca
      241 gcgggatctc tatcgccgag gacacgttct tgacccactc gagcctcgtc ttgcggtttt
      301 cctgcgtggc cgcgatgtcg agcatggcga gctcgtccgc gccctggtct tggtagaacc
      361 tcgcgtgttc gaccgggtct cccgcgtccc ggatgtcgac gaaatgcacg ccctttacga
      421 cccttccttc cagcatatcc aggcacggca tgatggcgac cggttccatg tcgggtctcc
      481 tccggaggtc atgatcttcg tgggttgaac cgtcagtccg ctcgagtgta cacgcaaggg
      541 cgtccacgaa tcaagcctct gcacgcggcg ttcgtgccac gtgcgacggc ccgaccgggt
      601 gccggggaac gatccatggg aaggcccgcg tctcgcatgc gcaagaggcc tcctctccga
      661 tcgggatgcg caaggccttg cgggggcgtc ggcccagggt gagaaatcga ggcgtcccgg
      721 tgacggacgt gtgaccgtcc ccggccggga ccgcgggcat gcatcaccgg cctgtccctt
      781 cagcggtgag ccccgctcgg gtcgagcccg tccgtccgcc cgctgtccca ggcgctgtcc
      841 ttggtgccct ggccaagctc tttgtcgtcc acgtactccg gcaggtacac cgtgaagcag
      901 gccccttggc cttcggcgct cgtgcaggtg accgccccct cgtgggcctt cacgatgccg
      961 tgcacgatgg agaggccgag ccccgagccc tcgcccttcc atgtcgtggt gaaatagggg
     1021 tcgaagaccc gcgagagggc gtaaccgggt atcccggggc cggtatcgct caccctcaac
     1081 acgatgcagc ggcccgcagg caaccccagc gcctccctct cgccctcggg gaccgtcgcc
     1141 cttgcgaggc tcacggtgat ctcgcctccg gtctttccca tggcatgggc ggcgttgtcg
     1201 cacaggttcg tgaagacctg ctccagctgg gccgggtcac ccttgaccat gcacgatgcc
     1261 gccaggttgg ccgaga
//
LOCUS       DAOSCR010000721         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_72488_length_1274_cov_2.340443, whole genome shotgun sequence.
ACCESSION   DAOSCR010000721 DAOSCR010000000
VERSION     DAOSCR010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72488_length_1274_cov_2.340443"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..407
                     /gene="rpoZ"
                     /locus_tag="PLT69_13080"
     CDS             213..407
                     /gene="rpoZ"
                     /locus_tag="PLT69_13080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOJ15420.1"
                     /translation="MARVTVEDCLEKVPDRFSLAIAAAKRTKQLMKGAVPLSRRKENR
                     YVVTALRELAEGKIAVHERS"
     gene            404..>1274
                     /gene="dnaJ"
                     /locus_tag="PLT69_13085"
     CDS             404..>1274
                     /gene="dnaJ"
                     /locus_tag="PLT69_13085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOJ15421.1"
                     /translation="MTKRDYYEILGVSRTATPEEIKKAYRQLALRYHPDRNPGDREAE
                     ARFKEAAEAYEVLSDPSKRRTYDQYGHEGLSRTGGFHEFTSFDDIFTSFSDIFGDMFG
                     FPGTRRGSRRRPVKGADLRYDAAITLEESAKGTEIELEIPKAFPCDSCGGTGAEAGTS
                     PERCATCGGKGQVYRSQGFFTISTTCPSCRGEGQVIKKPCRPCKGTGRVVHKRKLKVK
                     IPPGVDTGSTMRVPGEGELGEYGGPPGDLYVVVTVKPHEVFVRQGDDLYVEIPLSFVQ
                     AALGTTVKVPTLEG"
BASE COUNT          259 a          400 c          434 g          181 t
ORIGIN      
        1 caggcggcct caggggagcc cctcgtgaga ggatcccccg ggggtgaatc ccgtgcacac
       61 cggagacgct tttcccgatc cacacggacg tcaccccatg gcaggggatg cgggcgaaag
      121 gggcgctccc cagggggctg caggatatgc cttgactcgg gcgaacattt gtggcatgag
      181 ggcaaacacc tgaaaatccg ggaaggtata ccatggcacg agtcaccgta gaagactgtc
      241 tcgagaaggt tcctgatcgt ttctcgctcg ccatcgcagc ggccaagcgc acgaagcaac
      301 tcatgaaggg tgccgttccg ctgagcaggc gcaaggaaaa ccggtacgtg gtcaccgccc
      361 tgagggagct cgccgagggg aagatcgcgg tacacgagag gtcatgacca agagggatta
      421 ctacgagatt ctcggcgttt cccgaaccgc gacccccgag gagatcaaga aggcctaccg
      481 ccagctcgct ctcaggtatc accctgaccg caacccgggc gaccgggagg ccgaggcccg
      541 cttcaaggag gcggccgagg cctacgaggt cctgagcgac ccgtccaaga gacgcaccta
      601 cgaccagtac ggccacgagg gcctgtcccg gacgggcggc ttccacgagt tcacgagctt
      661 cgacgacatc ttcaccagct tctcggacat cttcggggac atgttcgggt tccccgggac
      721 gagaaggggt tcccgcagga ggcccgtaaa gggtgctgac ctcaggtacg acgccgccat
      781 caccctcgag gaatccgcga agggcaccga gatcgagctc gagataccca aggcattccc
      841 ctgtgactcc tgcgggggca ccggggccga ggccgggacc tcgcccgaga ggtgcgcgac
      901 ctgcgggggc aagggccagg tctacaggtc ccaggggttc ttcaccatca gcacgacgtg
      961 cccgagctgc aggggggaag gccaggtcat aaagaagccc tgcaggccgt gcaaggggac
     1021 ggggcgggtc gtgcacaagc ggaaactcaa ggtgaagata ccgccgggcg tggacaccgg
     1081 ctcgaccatg cgggtcccgg gcgagggcga gctcggggag tacggcggcc ccccggggga
     1141 tctctacgtg gtggtgacgg tcaagcccca cgaggtcttc gtcaggcagg gcgacgacct
     1201 ctatgtggaa atcccgctgt ccttcgtcca ggcggccctg ggcaccaccg tgaaggtccc
     1261 caccctcgaa ggcg
//
LOCUS       DAOSCR010000722         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_72827_length_1270_cov_2.960494, whole genome shotgun sequence.
ACCESSION   DAOSCR010000722 DAOSCR010000000
VERSION     DAOSCR010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72827_length_1270_cov_2.960494"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLT69_13090"
     CDS             <1..1134
                     /locus_tag="PLT69_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ15422.1"
                     /translation="SIRPMKRSGPRIGIALDSGGAMGGAHVGVLDVLAGEGFRPEVIV
                     GSSAGAAVGAVFAAGKLDEFASLISDMSFIKSLGLYVDPVFPYSGLLAGNRARAFLRE
                     LLGDAAIEDLPVKFAAVATDLLTGETVAIDSGPLADAVMASIAIPGIFRPVVHMGRLL
                     TDGGVSDPLPIDILRNYAPDIVIACNLHPRMTTRYSQANRRKIVEAERSARSLDDDLA
                     SGMIERVIGLVKSQIPSTGEVLKALPRALAERLTAENRGAILNSDIVKWLRETIDEKA
                     TFLDGIKELATVRPAEKWRMSIVDIILNVMNIQQYQKNRLMLKYEPPDILIEPDVVDI
                     AALEFNKSALAMEEGRRKAAEAVVELRRLVTRWGGQSGRSDPK"
BASE COUNT          243 a          411 c          431 g          185 t
ORIGIN      
        1 agcattcgcc ccatgaagag gagtggaccc cgcataggga tagccctgga cagcggcggt
       61 gccatgggcg gggcccacgt gggggtcctg gacgtcctcg caggcgaagg gttccggccg
      121 gaggtgatcg tggggtcgag cgccggtgcg gccgtgggcg ccgtcttcgc ggcggggaaa
      181 ctggacgagt tcgcgtcgct catctcggac atgtcgttca tcaagtccct gggcctctac
      241 gtcgacccgg tgttcccgta ctcgggtctc ctcgccggga acagggcccg tgcgtttttg
      301 agagaactcc tgggggatgc ggccatagag gacctgcccg tgaagttcgc cgcggtggcc
      361 acggaccttc tcacggggga gaccgtggcc atagactcgg gccccctggc ggacgcggtc
      421 atggcgagca tcgccatacc cgggatcttc aggcccgtcg tccacatggg gaggctgctc
      481 accgacggcg gcgtatcgga cccgctcccc atcgacatcc tcaggaatta cgccccggac
      541 atcgtgatcg cgtgcaacct ccacccgcgc atgaccacca ggtactcgca ggcgaacagg
      601 cgcaagatcg tggaggcgga gagaagcgcc cgctccctgg acgatgacct cgcatcgggc
      661 atgatcgagc gcgtcatagg gctcgtcaag tcccagatac cgtccacggg ggaggttctc
      721 aaggcgctgc cccgtgccct cgcggaaagg ctcacggccg aaaacagggg tgccatcctc
      781 aactccgaca tcgtgaaatg gctcagggag accatcgacg agaaagcgac cttcctcgac
      841 gggatcaagg agctcgccac cgtgaggccc gccgagaagt ggcggatgag catcgtcgat
      901 atcatcctga acgtgatgaa catccagcag taccagaaga accgcctcat gctcaagtac
      961 gaacccccgg acatcctcat cgagcccgac gtcgtggaca tagccgcgct cgagttcaac
     1021 aagagcgcgc tcgccatgga ggaaggcagg cgaaaggccg cagaggccgt tgtcgaactg
     1081 aggcgcctcg tgacgcgctg gggcggccag tcgggccggt cagaccccaa gtgaaggggg
     1141 agccggggcg agggacgaca gccccgccgg aaaccgtcgg aggccgcccg gcgacccgga
     1201 cgggccggaa acctgaagtg ttgcccccct gagacgctcc cacgcggatc ggggcggccc
     1261 ttccagagac
//
LOCUS       DAOSCR010000723         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_73336_length_1264_cov_2.789082, whole genome shotgun sequence.
ACCESSION   DAOSCR010000723 DAOSCR010000000
VERSION     DAOSCR010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73336_length_1264_cov_2.789082"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PLT69_13095"
     CDS             complement(<1..1208)
                     /locus_tag="PLT69_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOJ15423.1"
                     /translation="MNREQIDRDIEAIYNLGFFSYVDVRLETRPDGVKVVYVVQENPP
                     VKEIRIEGNSIYSDEELMKEVFTVPGSIFNRVFFRHDLERIQNKYREAGYVLVRMQDV
                     RFENGIVEVQIVEPRVGEIIIQGNTRTQTNVIRREIPIKEGDVFNATYMRHSLNKLQR
                     LGYFEDVNVGFEPTDDPEVMNIVITVTEQRTGRIGVSLGHGSSSGWSGGLVYEDTNWQ
                     GKGHRAVVGFETGDREQYWISYEEPFMDEEYYAWKVGVYKHQWEDIDYIDDDGDTVFE
                     YDEDRTGYYIGAGRKFKHDPRMNWYVTLDWHDSSVDPTWWHSEEPVDYEAVRNLLSSG
                     KTFSVLGTVQRTNTDRYLSYPKGDVEILNIQQAMSIMGSDWDYTKYWIEGRYYTPLTF
                     LQDMFGAELGT"
BASE COUNT          252 a          385 c          291 g          336 t
ORIGIN      
        1 gtgccgagct ccgccccgaa catatcctgc agaaaggtaa gtggtgtata atatcgcccc
       61 tctatccagt atttggtata gtcccagtcg cttcccatta tgctcatggc ctgctgtatg
      121 ttgagaattt ccacgtcgcc tttgggatag ctcagatacc tgtccgtgtt tgtcctttga
      181 accgtcccca agacggaaaa agtcttaccg ctgctcaaaa ggttcctgac agcctcatag
      241 tctacgggct cttcggagtg ccaccaggtg gggtctaccg aagagtcgtg ccaatcgagc
      301 gtcacatacc agttcatgcg ggggtcgtgc ttgaactttc tgccggcgcc gatgtaatag
      361 ccggtcctgt cttcatcgta ttcgaaaacc gtgtcgccat cgtcgtcgat gtagtcgata
      421 tcttcccatt ggtgcttgta aactccgacc ttccaggcgt aatactcctc gtccataaaa
      481 ggctcctcgt aggagatcca atattgttcg cggtcacccg tctcgaaacc cacgacggcc
      541 ctgtgcccct ttccctgcca gtttgtgtct tcgtacacca agccgccgct ccaaccgctg
      601 ctcgagccgt ggcccaggga gacgcctatg cgccccgtcc tctgctctgt gacggttatg
      661 actatattca tcacttcagg atcatcggta ggctcaaagc ccacgttcac gtcttcgaaa
      721 taccccaacc tctggagctt gttgagggaa tggcgcatgt atgtggcgtt gaagacatcg
      781 ccttctttta tcggtatctc cctgcggatc acgttcgtct gcgtcctcgt gttgccctgt
      841 attatgatct ctcccacgcg gggctccact atctgcactt cgacgatccc gttttcgaag
      901 cgcacatcct gcatcctcac gagaacatag ccggcttccc tatatttatt ctgaatgcgc
      961 tccaagtcgt gccggaagaa gacgcggttg aagatgcttc cagggacagt gaagacctct
     1021 ttcatcagct cttcatcgct atatattgaa ttgccctcaa tcctgatctc cttcacggga
     1081 ggattttcct gcacaacata gacgaccttc acgccgtcgg gcctggtctc caacctgacg
     1141 tctacgtatg aaaaaaaccc caagttgtag atggcctcta tgtccctgtc gatctgttcc
     1201 ctgttcaagg gatcccccac cttggtcccg atcaccccga ggatgtactg cgaggctatc
     1261 tccc
//
LOCUS       DAOSCR010000724         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74050_length_1256_cov_2.781848, whole genome shotgun sequence.
ACCESSION   DAOSCR010000724 DAOSCR010000000
VERSION     DAOSCR010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74050_length_1256_cov_2.781848"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1131
                     /locus_tag="PLT69_13100"
     CDS             316..1131
                     /locus_tag="PLT69_13100"
                     /inference="COORDINATES: protein motif:HMM:NF018699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HOJ15424.1"
                     /translation="MTVKELIREARLGEARSVLIEEVKQRPGDTRARTLLFQVLALLG
                     EWEKAARHLEMIAPAGGAHAGVSAFMDITKAELERREVIGEWKAPPVVPGVPPYIEEY
                     LAYLDALKERDETGARELLGRIDSARPRVSGTINGEPFSDFTETDARLYAFLEAFVHD
                     RYVWVPFEAIREIVLAEPASSFDLIWAGADIVTWEGLSLRCYLPVTYPDTSSLGDDEA
                     RLGRKTTWERICGGLSMGVGQHVYQAGDREVALLDIRTGEFTMEVEGHGGKDA"
BASE COUNT          219 a          363 c          470 g          204 t
ORIGIN      
        1 ggcgaggtcg tcgagttggg cgaggtcgat catcgaggcg gtcttcatgg gcgggtcata
       61 tgatgccgcc gaggtcgcgg ccttcgagag gggcctggtg gagagagatt tcgtgcgcct
      121 gtggaacgtg tgcgcgggcg gcaaggtatg cgctggcgct ccctgaggcg ggctctccgg
      181 ccccgaaggc cagggggcgc ggtcatccgg ctgtcggcgt cgttgggtgg ggacgggcgg
      241 tcttgatccg ggccgacgcg aagctatcat tcgccaagtg gatatggtag ggaaaagaca
      301 gaggatgtat ttgtcatgac cgtgaaggag ctcatcaggg aggctcgcct cggcgaggcc
      361 cgctcggtgc tgatcgagga ggtgaagcag aggcccgggg acacgcgcgc ccggaccctc
      421 ctcttccagg tcctggccct cttgggcgag tgggagaagg ccgcccgcca cctggagatg
      481 atcgcccccg ccggcggcgc acacgccggg gtgtccgcct tcatggacat aaccaaggcg
      541 gagctcgaga ggcgcgaggt gatcggggag tggaaggcgc ctcccgtcgt acccggtgtg
      601 ccgccttaca tcgaggagta tctcgcgtac ctggatgcgc tcaaggagcg cgacgagacc
      661 ggcgcgaggg aactgctcgg gcgcatcgac agcgcccggc cgagggtgtc ggggacgatc
      721 aacggcgagc ctttctccga cttcacggaa acggacgcac gcctgtacgc cttcctcgag
      781 gccttcgtgc acgacaggta cgtgtgggtc cccttcgaag cgatccgtga gatcgtcctt
      841 gccgagcccg cctcgtcctt cgacctcatc tgggcgggcg ccgatatcgt gacatgggaa
      901 gggctctcgc tcaggtgtta cctgccggtg acctaccccg atacgtcgag cctcggcgac
      961 gacgaggcca ggctcgggag gaagaccacc tgggaacgga tctgcggagg gttgtccatg
     1021 ggggtggggc agcacgtata ccaggcaggg gacagggaag tggctctcct cgacatcagg
     1081 accggggagt ttaccatgga ggtggaggga catggcggga aggacgcctg aagtagagct
     1141 cctgctgaag cctatcgacg gcgggaaccc ctccggcggg gacctgcggt acagcgaggt
     1201 gtatgaccgc atcaaggagg ccaggcgcgc cgacgacatg ctcaaccagg gcgact
//
LOCUS       DAOSCR010000725         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74160_length_1255_cov_2.326667, whole genome shotgun sequence.
ACCESSION   DAOSCR010000725 DAOSCR010000000
VERSION     DAOSCR010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74160_length_1255_cov_2.326667"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLT69_13105"
     CDS             complement(<1..309)
                     /locus_tag="PLT69_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15425.1"
                     /translation="MKVRTIPKTTSLATAGTGAPPQGRARIPWDAAGAGLPNILDMDK
                     HGLRGLFAGEPYRGDQVYGWLFSKGVQDFNDMTDLPKDLRARLASQWAIAYPEVVSHK
                     V"
     gene            complement(365..441)
                     /locus_tag="PLT69_13110"
     tRNA            complement(365..441)
                     /locus_tag="PLT69_13110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Arg,seq:ccg)
     gene            complement(449..>1255)
                     /locus_tag="PLT69_13115"
     CDS             complement(449..>1255)
                     /locus_tag="PLT69_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15426.1"
                     /translation="RHDGGDRWIGTGGTSPVGHSGYHPGGMRVGGSSRMKSAVKVALE
                     RRYKDYTEDTRLGPSQISDALKRLRHMVPAGPKDRLSIEKTIRATMKNAGEIEIVFDA
                     SLKDKLKVILMIDNGGFSMDPYVHVVQVLFNHARNNFKDLQVFYFHNTIYNRVWRDPQ
                     RYEKPVYLEELARLDPETRLIIVGDASMAPYELFSRNGSIMYNSKSDPSIVKLEFLAK
                     TFRHSVWLNPKYEYTWAHTQTIGAIREIFPMFEITLKGLEEAVGYLTRRN"
BASE COUNT          193 a          391 c          400 g          271 t
ORIGIN      
        1 caccttgtgc gagaccacct cgggatacgc tatggcccat tgggaagcga gccgggcccg
       61 caggtccttc ggcaggtcgg tcatgtcgtt gaagtcttgc acccccttcg agaagagcca
      121 gccgtacacc tggtcacccc ggtagggttc gcctgcgaac agccccctga ggccgtgctt
      181 gtccatgtcg aggatgttgg gaagccccgc gccggcggcg tcccacggga tgcgggcacg
      241 gccttgcggc ggggcgcccg tacccgccgt ggcgagcgag gtcgtcttcg gtatggttcg
      301 caccttcatg agcactggga aagggttcag gacccttgag cctcgccggg cgaggcgttc
      361 gtcgtggcgc gcccgacagg gatcgaacct gtgaccttag gctccggagg cctacgctct
      421 atcctactga gctacgggcg cgcacccgtc agttccttct cgtcaagtac cccacggcct
      481 cctccagccc cttgagcgtg atctcgaaca tggggaagat ctcccgtatc gcgcctatgg
      541 tctgcgtgtg ggcccaggtg tactcgtatt tcgggttgag ccatacacta tgtctgaacg
      601 tctttgcaag gaattccagc ttcacgatgc tggggtcgct cttggagttg tacatgatgg
      661 agccgttgcg gctgaagagc tcgtaggggg ccatggacgc gtcacccacg atgatgagcc
      721 tggtttccgg gtcgagcctc gcgagctctt ccaggtacac gggcttctcg taccgctgcg
      781 ggtctctcca gacgcggttg tatatggtgt tgtggaagta gaacacctga agatccttga
      841 agttgttcct cgcgtggttg aacaggacct gcaccacgtg gacgtacggg tccatggaga
      901 acccgccgtt gtcgatcatg aggatgacct tgagcttgtc cttcaaggag gcgtcgaaca
      961 cgatctctat ctcgcccgcg ttcttcatgg tggcccggat ggtcttctct atgctcaggc
     1021 ggtctttggg gcccgcgggc accatgtgcc tgagtctctt gagggcgtcc gagatctgtg
     1081 acgggccgag cctggtgtcc tcggtatagt ctttgtacct gcgttccagg gccaccttga
     1141 ccgcggactt catccgcgac gacccgccca cgcgcatgcc ccccgggtgg taccccgagt
     1201 ggcccacggg cgaggtgccg ccggtgccga tccacctgtc gcccccgtcg tggcg
//
LOCUS       DAOSCR010000726         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74376_length_1252_cov_2.767753, whole genome shotgun sequence.
ACCESSION   DAOSCR010000726 DAOSCR010000000
VERSION     DAOSCR010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74376_length_1252_cov_2.767753"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLT69_13120"
     CDS             <1..225
                     /locus_tag="PLT69_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ15427.1"
                     /translation="RESGAIEQDADVICFIYREDFYTKDKGMPPTGIAEVIVAKHRNG
                     PTGTVELVWMPEFTRFGDLDVKHQEPQGKR"
     gene            883..>1252
                     /locus_tag="PLT69_13125"
     CDS             883..>1252
                     /locus_tag="PLT69_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632290.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOJ15428.1"
                     /translation="MKKGSKTREPLRFVTATALFDGHDASINIMRRVLQDAGAEVVHL
                     GHNRSALEIVNAAIQEGAHAVAVSSYQGGHLEFFKYLVDLLADKGCGYVKVFGGGGGV
                     IVPHEIRELEAGLIKLEEDQS"
BASE COUNT          257 a          344 c          460 g          191 t
ORIGIN      
        1 agggagtccg gcgcgataga gcaggacgcg gacgtgatct gcttcatcta cagggaagat
       61 ttctacacca aggacaaggg catgccgccg accggcatcg ccgaggtcat cgtggccaag
      121 cacaggaacg gccccaccgg cacggtcgaa ctcgtgtgga tgccggagtt cacgaggttc
      181 ggagacctcg acgtcaagca ccaggagccg caaggcaagc gttgaggagc gcgcgggcag
      241 gttcccgccc gcacctgaag accttcttct tgaaaagaac gacggaaggc atgcgacgca
      301 aggcgtgcgc cgcgaccgtg aagggccgtg tcgcccgtga caatgccaag aaggggatgg
      361 gggaagagaa aacgaaaggt cacgaaggcc gtgctcgggg cagaggggtc gccctgaaca
      421 cgcatctacc gggggcaggc cagtaggggt cgcaccgcag gtacacgggg ggcggggaat
      481 gacccgcgcg ggcggccggg gggcctcgcc ggggtatggt gtttgggacg atgtatccgt
      541 caggctcttc gtggggagtg atccacgagg gctgccggcg gtcacgctga ggcggggctg
      601 acgcacgggg aagtggaggg tgcggccgtt cagcgacagg atccttgtcg tgccgcttcg
      661 ttccagggca tgcctcgagt gggcccacga ggtcgcgggc gatccgcact ggtgaaagca
      721 ggggagacca cctccggccg ttcaagggga agacatgtcc cgactgcgta gtgattcggg
      781 atgcactcgc gatgtcgcgc ctgcatgagg aaatagaaat ggttgcatcg atatggtaat
      841 ggaagttgag taagcatgta tgacaagggt ggggtctgaa ccatgaagaa gggatcgaag
      901 acgagggaac ctctccgttt cgtgaccgcc acggcgctgt tcgacggcca cgacgcatcc
      961 atcaacatca tgcgccgcgt cctccaggac gccggcgcgg aggtcgtaca cctgggtcac
     1021 aaccgttcgg cgctcgagat cgtgaacgcg gcgatccagg aaggggccca cgcggtggcg
     1081 gtcagctcct accagggcgg gcacctcgag ttcttcaagt acctcgtgga cctgctcgcc
     1141 gacaaggggt gcggttacgt caaggtcttc ggcggcgggg gcggggtcat cgtgccccac
     1201 gagatcaggg agctcgaggc ggggctcatc aagctcgagg aagatcagag cg
//
LOCUS       DAOSCR010000727         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74451_length_1251_cov_3.020903, whole genome shotgun sequence.
ACCESSION   DAOSCR010000727 DAOSCR010000000
VERSION     DAOSCR010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74451_length_1251_cov_3.020903"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLT69_13130"
     CDS             <1..740
                     /locus_tag="PLT69_13130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ15429.1"
                     /translation="RNIEACLQSVTWADEILVVDSFSTDTTLEIARRYTDRILRHEYV
                     YSAAQKNWAIPKASNEWVLVVDADERVTPGLRQEIADLLEQGPSFDGYWIPRRNFLFG
                     REIRHSGWGSDSVLRLFKRDVGRYRDKRVHAEVEISPAGRLRHHLEHHSVPSLADWVA
                     KINRYSTWKARDKHEKGMAAPLVHLVVRPPARFLKDFVLRRGFMDGWRGFVIASLSAF
                     AELVMAAKVMELRMEESERNPGSGKPG"
     gene            737..>1251
                     /locus_tag="PLT69_13135"
     CDS             737..>1251
                     /locus_tag="PLT69_13135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ15430.1"
                     /translation="MRIVHVNLERTWRGGERQVVYLMEGLARLGHECHLVARKNGLLA
                     GRAREKGIPVLEVRKPFVARGFVLGGFDVVHAHETRALQMAALWKGWNTSPVVFTRRV
                     DNPPSTNPVTRLVYGKIDRMAVISRRVGEVMASWGFDPGRTRVIPSSVHPLGDPDDAT
                     VSGLRRRFDGR"
BASE COUNT          243 a          355 c          452 g          201 t
ORIGIN      
        1 agcgcaacat cgaggcctgc ctccagagcg tgacctgggc tgacgagatc ctcgtcgtcg
       61 actcgttcag caccgacacg acgctcgaga tcgcgaggag gtacacggat cgcatcctcc
      121 ggcacgagta cgtctattcg gctgcgcaga agaactgggc gataccgaag gcgtcgaacg
      181 agtgggtgct cgtcgtggat gcggacgaga gggtcactcc cggcctcagg caggagatcg
      241 cggacctgct cgagcagggg ccctccttcg acgggtactg gatcccgagg agaaacttcc
      301 tcttcggcag ggagataagg cattcgggat ggggctcgga cagcgtgctg aggctcttca
      361 agagggacgt ggggaggtac cgggacaaac gggtccacgc ggaggtggag atcagtcccg
      421 caggccgcct gaggcaccat ctcgagcacc acagcgtccc atcgcttgcg gactgggtcg
      481 ccaagatcaa caggtattcg acctggaagg ccagggacaa gcacgaaaag ggcatggccg
      541 cgccactcgt gcacctggtg gtacggccgc ccgccaggtt cctcaaggac ttcgtcctga
      601 ggagagggtt catggacggc tggaggggtt tcgtcatcgc gtccttgagc gcgttcgcag
      661 agctcgtcat ggcggccaag gtgatggaac tcaggatgga ggagagcgaa cgaaaccccg
      721 gttcggggaa gcccggatga ggatcgttca cgtgaacctc gagcgcacct ggcggggcgg
      781 ggaacggcag gtcgtgtatc tcatggaagg gctcgcccgg cttggccacg agtgtcatct
      841 cgtggcgagg aagaacgggc tcctggcagg gcgggcaagg gagaagggca taccggtgct
      901 ggaggtgcgt aagccgttcg tcgcccgggg ctttgtgctc ggagggttcg acgtggttca
      961 cgcccacgag acgagggctc tgcagatggc cgccctctgg aaggggtgga acacatcgcc
     1021 cgtcgtgttc acccggaggg tggacaaccc cccatcgaca aacccggtga cccgcctcgt
     1081 ctatggaaag atagacagga tggccgtgat ctcccgcagg gtgggcgagg tcatggcgag
     1141 ctggggcttc gatcccggcc gcacgagggt tatcccgagt tccgtccacc ccctggggga
     1201 tccggacgat gcgacggtca gcgggctcag gcgaaggttc gacggcagga a
//
LOCUS       DAOSCR010000728         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74560_length_1250_cov_2.451883, whole genome shotgun sequence.
ACCESSION   DAOSCR010000728 DAOSCR010000000
VERSION     DAOSCR010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74560_length_1250_cov_2.451883"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..481)
                     /locus_tag="PLT69_13140"
     CDS             complement(251..481)
                     /locus_tag="PLT69_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15431.1"
                     /translation="MDPDTTKKVDDLLGMVEGISSEIDVKAELDRIVERLGAIEQKVE
                     RLARRVEALERDQRERKKAAQKLKEELEDLAR"
     gene            complement(471..>1250)
                     /locus_tag="PLT69_13145"
     CDS             complement(471..>1250)
                     /locus_tag="PLT69_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15432.1"
                     /translation="DPVLIREQTDEERAETQTPVAAAEAQPNPEAFQEVEGAKAQAAA
                     ATADARPAPAPQASVSTQVGTTSVDMVSMAVETIEDINAEFSQILFERRDIKRRLEEH
                     DRIVEEYERENERLRENIARLERTAGEHPHLSREITFLNEQIEDADHYIRRVMTMLGT
                     VNASYDSELAKSRALEERLERMSRDLKEKARLDVKVSILEKDLSFSTARIRELESRLE
                     EEAMKRRPLEEEIAELKSALDKVYSSLSHIRLKAKREVYGS"
BASE COUNT          154 a          437 c          390 g          269 t
ORIGIN      
        1 ccacggccct cggcaccctt tctcaccggg caccgggccc ggcagtcgag catggggcgg
       61 cctaggggac acggcctcgg ttttcaacag gcgggaactt caccggcacg acacgcggtg
      121 cagccccggc gccacgaacg accggcgtct cgcccgctgt gacgcccaca tcgcaaagga
      181 cttgcccgcg cccatcgttg gcgccacgga cttttgacgc cccgcccgct cccacggcga
      241 gcggggctgt tcacctggcg aggtcctcga gctcctcctt cagcttctgg gcggccttct
      301 tccgctcccg ctggtcgcgc tccagggcct ccacgcgcct ggcgagcctc tcgaccttct
      361 gctcgatcgc tcccagacgc tccacgatcc tgtccagctc cgccttgacg tcgatctcgg
      421 aagagatccc ctctaccatt ccgagcaggt cgtcgacctt cttggtggtg tcaggatcca
      481 tacacctcgc gtttcgcctt gaggcggatg tgcgagaggg aggagtagac cttgtcgagt
      541 gcgctcttga gctctgcgat ctcttcctcg aggggtctcc tcttcatggc ctcctcctcg
      601 agcctgctct ccagctcgcg gatgcgggcc gtggagaagc tcaggtcctt ctcgaggatc
      661 gagaccttca cgtccagcct ggccttttcc ttgaggtccc tgctcatgcg ctcgaggcgc
      721 tcctcgaggg ccctgctctt ggcgagttca gagtcgtagc tggcgttgac ggtgccgagc
      781 atggtcatga cgcgcctgat gtagtggtcg gcgtcctcga tctgctcgtt gaggaaggtg
      841 atctcccggc tcaggtgggg atgctccccg gcggtgcgct cgagcctggc gatgttctcc
      901 ctgagcctct cgttctcgcg ctcgtactcc tccacgatac ggtcgtgttc ctcgagcctg
      961 cgcttgatat ccctcctctc gaagaggatc tgggagaact cggcgttgat gtcctcgatg
     1021 gtctccacgg ccatggagac catgtcgacc gaggttgtgc ccacctgcgt ggaaaccgag
     1081 gcctgtggcg ccggtgcggg ccgggcgtcc gccgtcgccg ctgcggcctg cgcctttgcg
     1141 ccctcgacct cctggaaggc ctcggggttc ggctgcgcct cggctgcggc gacgggggtc
     1201 tgggtctcgg cccgttcctc gtcggtttgt tcccgtatga gtaccggatc
//
LOCUS       DAOSCR010000729         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74645_length_1249_cov_3.043551, whole genome shotgun sequence.
ACCESSION   DAOSCR010000729 DAOSCR010000000
VERSION     DAOSCR010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74645_length_1249_cov_3.043551"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLT69_13150"
     CDS             complement(<1..1035)
                     /locus_tag="PLT69_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOJ15433.1"
                     /translation="MRVQSIRVKINASIIAACIIVALFFGVVFSSFEIQRRASRIQEI
                     GTLMEAVYEQNREELANEIFARHTEALASTLDAITSVKGICHIAVYDLEGRIIMATGK
                     TVQRLMAQSTRKVLNRGPSVREVSISKEAYLEYASRIEVIGERVGYIAMYYDLTGLEE
                     EGARTGMAFLALLVTTLVVLSLVLNRILTGAVLSPMTGLRQAIQKLQLGNLGEQVSVT
                     SKDEIGEVMCAFNDFSVMLLEQRQALDHSLKMQKQYAKELEDANRMLEHLNVRLEDMV
                     RERTRELLAANEQLKEQIAERRRSEKARKELDDRLARSEKMEALGLLAGGVAHDLNNV
                     LSGIVSYPDLL"
BASE COUNT          208 a          389 c          386 g          266 t
ORIGIN      
        1 gaggaggtca gggtagctca cgatgcccga gagcacgttg ttgaggtcgt gggccacgcc
       61 ccctgccaag agcccgaggg cctccatctt ctcggagcgt gcgagcctgt cgtccagctc
      121 tttccgcgcc ttctcggacc tcctgcgctc ggcgatctgc tctttgagct gctcgttggc
      181 cgcaaggagc tccctcgtcc gttcccgcac catgtcctcg agacgcacgt tcaggtgctc
      241 gagcatcctg ttcgcatcct ccagttcctt ggcgtactgc ttctgcatct tcagggaatg
      301 gtcgagcgcc tggcgctgct ccaggagcat gacggaaaag tcgttgaagg cgcacatcac
      361 ctcgccgatc tcgtctttgg aagtgaccga gacctgctcc ccgagattcc cgagctggag
      421 tttctgtatg gcctgcctga gcccggtcat cggggaaagg accgccccgg tcaggatcct
      481 gttcaggacg agggagagga ccacgagcgt cgtgaccaag agcgccagga acgccatacc
      541 ggtccgtgcc ccttcctcct cgagtcccgt gaggtcgtag tacatcgcga tgtatcccac
      601 ccgctcgccg atgacctcta tacgggatgc atactccagg tacgcctcct tcgagatcga
      661 gacctcgcgc accgaaggcc ccctgttcag gaccttccgt gtgctttgcg ccatgagtct
      721 ctgcacggtc ttgcccgtgg ccatgatgat gcggccttcc aggtcgtaga ccgcgatgtg
      781 gcagatgccc ttgacggagg tgatggcgtc cagggtggat gccagtgcct cggtgtgccg
      841 tgcgaagatc tcgttggcga gttcctcgcg gttctgttcg tagacggcct ccatgagggt
      901 gcctatctcc tggatacgcg atgcgcggcg ctggatctcg aacgaggaga acactacccc
      961 gaagaagagc gccacgatga tgcacgcggc tatgatcgag gcgtttatct tgaccctgat
     1021 actctgtact ctcatggcga tcgagaccct gctcatcgtc ttttcggaag ggcagacgcg
     1081 agagttgagg tctcgtgcat cggcccgtgc acgcctgggc ggccccgacg tcgtgttccg
     1141 gatggcgacc ccttgatggg actgcgcccg tgtcaaaggg gtttccttgc atgtcgtgac
     1201 gggaaagggt gaccggggtt acacccgggc acagacggag aggaccctt
//
LOCUS       DAOSCR010000730         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_74924_length_1246_cov_2.736356, whole genome shotgun sequence.
ACCESSION   DAOSCR010000730 DAOSCR010000000
VERSION     DAOSCR010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74924_length_1246_cov_2.736356"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1246)
                     /locus_tag="PLT69_13155"
     CDS             complement(5..>1246)
                     /locus_tag="PLT69_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ15434.1"
                     /translation="RRMYLVCDGNPPARIPRHVMGVLPKDASVLNAMAQLYREMRQSF
                     ELGDMLITSLGEKDQQIKHNRRQLLANSRRYNAIVQNANDIIFIVGRTGRITFCNEKF
                     TRCLAVGGTSPVGTSITRYAVEEDRSELGKALARGFDECAPVRLEVRMPLASGRVGIL
                     SLLFTPLVEEGRAYALSVIGRDVTELRAMQKRLAIQAGDLSQMMNGLAHELRNPLTVI
                     GAYVRRLVRSPGEDKRKWDEALSGIYSSIGRIEAMIERVEAYERLVGMDAACVPLDLE
                     KLVTSTVAEAGAGIPVEVDVQPDVRALGDEEHVRTALVRILENALQTSTPKVVVKVWR
                     DDAYAYVSVRDFGPGVKDKVQTILSPFYSTDPMKIGLGLTEARIAMVKIRGDLEVVRQ
                     AGPGAVFTLKLLLDMRKSERS"
BASE COUNT          195 a          438 c          373 g          240 t
ORIGIN      
        1 gttctcaaga ccgttccgac ttcctcatgt cgaggaggag cttgagcgtg aacaccgccc
       61 ccgggcccgc ctgccttacg acctcgagat cccccctgat cttgaccatg gcgatgcgcg
      121 cctcggtgag gccgaggccg atcttcatcg ggtccgtgga atagaacggg gaaaggatgg
      181 tctgcacctt gtccttcacc cctggcccga agtcgcggac cgagacgtac gcgtaggcat
      241 cgtcgcgcca caccttcacg accaccttcg gcgtcgacgt ctggagggcg ttctccagta
      301 tcctcacgag cgcggtccgc acgtgttcct cgtccccgag ggcccggacg tccggctgca
      361 cgtcgacctc cacgggaatc ccggcccccg cctccgccac ggtcgaggtc accagcttct
      421 cgaggtcgag gggcacacac gcggcatcca tgccgacgag cctctcgtac gcctcgaccc
      481 gctcgatcat ggcctcgatg cgtcctatgg aggagtagat gcccgagagg gcctcgtccc
      541 acttcctctt gtcttccccg ggcgatctca cgagccttcg cacgtaggcg ccgatcacgg
      601 tgagggggtt tctcagctcg tgtgcgagcc cgttcatcat ctggctcagg tctccggcct
      661 gtatggcgag cctcttctgc atcgccctca gctccgtgac gtcacgcccg atgacggaca
      721 aggcatacgc gcgcccctct tccacgagcg gggtgaacag cagcgacagt atccccaccc
      781 tgccggacgc aagcggcatc cgcacctcga ggcggacggg cgcgcactcg tcgaaccccc
      841 tggcgagggc ctttcccagc tcgcttctat cctcctccac cgcatatcgc gtgatactgg
      901 tccccaccgg cgaggtcccg ccgacggcga ggcaccgcgt gaacttctcg ttgcagaagg
      961 tgatcctgcc ggtgcgcccg acgatgaaga tgatatcgtt cgcgttctgt acgatggcgt
     1021 tgtaccgcct gctgttcgcg agcagctgcc tgcggttgtg cttgatctgc tgatccttct
     1081 cgccgagcga cgtgatgagc atgtccccca gttcgaacga ctgcctcatc tcgcggtaga
     1141 gctgcgccat ggcgttgagc accgaggcgt ccttgggaag cacgcccatg acgtggcggg
     1201 ggatcctggc aggggggttc ccgtcgcaga cgaggtacat cctcct
//
LOCUS       DAOSCR010000731         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_75062_length_1244_cov_3.664424, whole genome shotgun sequence.
ACCESSION   DAOSCR010000731 DAOSCR010000000
VERSION     DAOSCR010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75062_length_1244_cov_3.664424"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1242
                     /locus_tag="PLT69_13160"
     CDS             103..1242
                     /locus_tag="PLT69_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOJ15435.1"
                     /translation="MPHQLNAEQEMIRLMARDFARKELEPYAAERDREGIFPLDVVKK
                     MGDLGFLGMMVPPEYGGAGAGAVSYCLAVQEIAYSCASTAVTMSVANLSTEPLLKFGD
                     ESQKRRYLERLARGELLGCFALTEPGAGSDPGSLRLRAEDRGDHFLLNGTKVFITHGA
                     YADVVNLIARTGPDKGNKGLSAFIVERGTPGFRVGATEKKMGLRASNTVELVFEDCKV
                     PRENLVGRPGLGFTIAMTALDSGRIGIASQATGIARACLDEAVAYARQRRQFGRTIGS
                     FQAIQWMIADMATSIEASHLLTLEAARSKDLGLPFTSQASMAKLFASETANKAAYHCL
                     QIHGGYGYTMDFKVERLYRDAQATTIYEGTSEIQRLVIARRFLKE"
BASE COUNT          250 a          398 c          409 g          187 t
ORIGIN      
        1 cgagcatcca cttcaactcc gcgctccacc acacgctcag caacccgtcg gacctcaaga
       61 ccgagctcct cgtggtcata tacgtaccgt aggaggcgcg cagtgcccca ccaactcaac
      121 gccgaacagg aaatgatcag gctgatggcc cgcgacttcg cccggaagga gctcgaaccg
      181 tatgcggcag agcgcgacag ggaagggatc ttccctctgg acgtggtgaa gaagatgggg
      241 gaccttgggt tcctgggcat gatggtgccc ccggaatacg gcggcgccgg ggccggcgcc
      301 gtgagctact gcctggcggt acaggagatc gcgtattcct gcgcctccac cgcggtcacc
      361 atgtcggttg cgaacctgtc caccgaaccc ctgctcaagt tcggggacga atcccagaag
      421 cgccgctacc tcgagaggct ggccaggggg gaactgctcg gctgcttcgc gctgaccgag
      481 ccgggtgcag gttccgaccc cgggagcctc aggctcaggg cagaagacag gggcgatcac
      541 ttcctcctga acggcaccaa ggtgttcatc acccacggcg cgtacgctga cgtcgtcaat
      601 cttatcgcca ggacagggcc ggacaagggg aacaagggac tgagcgcctt catcgtggag
      661 agggggaccc cagggttccg cgtgggggcg acggagaaga agatgggcct gagggcttcc
      721 aacacggtgg aactcgtgtt cgaggactgc aaggtcccga gggagaacct cgtcgggagg
      781 cccggcctcg ggttcaccat cgccatgacc gcgctcgaca gcggccgcat agggatcgcg
      841 tcgcaggcca cggggatcgc gcgagcctgc ctggacgagg cggtcgcgta cgcgaggcag
      901 aggaggcagt tcggcaggac catcggctcc ttccaggcaa tccagtggat gatcgcggac
      961 atggccacct ccatcgaggc gtcgcacctg ctgaccctcg aggcggcgag gagcaaggat
     1021 ctcggcctcc cgttcacctc gcaggcctcc atggcgaagc tcttcgcatc tgagacggcg
     1081 aacaaggccg cctaccactg cctccagatc cacggcgggt acgggtacac catggacttc
     1141 aaggtggaga ggctctaccg agacgcgcag gccacgacca tctacgaagg cacatccgag
     1201 atccagcgcc tcgtcatcgc aaggcgtttc ctcaaggagt gaag
//
LOCUS       DAOSCR010000732         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_75345_length_1241_cov_2.998314, whole genome shotgun sequence.
ACCESSION   DAOSCR010000732 DAOSCR010000000
VERSION     DAOSCR010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75345_length_1241_cov_2.998314"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..965)
                     /locus_tag="PLT69_13165"
     CDS             complement(60..965)
                     /locus_tag="PLT69_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOJ15436.1"
                     /translation="MFATRRILPAAILVALLAPSLHAGQVVMTDDERSWARDALRQEA
                     SAQARPGKNTVAVLAFRSRSQGPDITPLQKGFAYLLQTDLSRIEGITVVERLKIQALL
                     EELDLGASGLVEEGTAPRVGRLLGARFLVGGELAASSGQDLKVRSDVLQVPDQSTLGS
                     PSAEGMLEQVFDIEKKILFEVVSLLKVKLSARERQELSRAPTQNYRAFVLFCKGIDAA
                     DRGNLAAARNYFDRALALDPAFTPARNAVEELTTRGLSPKKPGSSALIEAQAEQNSST
                     LSITRTTATIREFRPSATGQIRVTW"
BASE COUNT          191 a          385 c          444 g          221 t
ORIGIN      
        1 gggcgggggc gggtttctct gtgggtgcgg gccgagggat gttccatgtc aagccctcct
       61 taccatgtca cgcggatctg gccggtggcc gaaggcctga actccctgat cgtcgccgtg
      121 gtcctcgtga tggagagggt cgaggaattc tgttcggcct gtgcctcgat cagggcgctg
      181 gaaccgggtt tcttggggga gagccccctg gtggtgagct cttccacggc gttgcgggct
      241 ggggtgaacg cggggtcgag cgcgagggcc ctgtcgaaat agttccgtgc cgcggcgagg
      301 ttgcccctgt ccgcggcgtc gatgcccttg cagaagagga cgaaggcgcg gtagttttgc
      361 gtcggcgccc tggagagctc ctgtctctcc ctggcggaca gcttgacctt gaggagggag
      421 accacctcga agaggatctt cttctcgatg tcgaagacct gttcgagcat gccctcggcc
      481 gaggggctgc cgagggtgct ttggtcgggc acctggagga cgtcggagcg gaccttgagg
      541 tcttgcccgg aggaagccgc gagctctccc cccaccagga agcgggcccc cagcagcctc
      601 ccgacgcgcg gcgcggtccc ctcttccacg agcccggatg caccgaggtc gagctcctcg
      661 agcaaggcct ggatcttcag ccgctccacc acggtgatcc cctcgatgcg ggagaggtcg
      721 gtctggagga ggtaggcgaa gcccttctgg agcggggtga tgtcggggcc ttgcgaacgg
      781 ctcctgaagg cgagcaccgc gacggtgttt ttccccggcc tggcctgcgc ggacgcctcc
      841 tgccggaggg catccctggc ccacgaccgt tcgtcgtccg tcatgacgac ctggcccgca
      901 tgcagtgagg gcgcgagcag cgccacgagg atcgcggccg gtagtattcg gcgtgtcgcg
      961 aacatgtcac accccctcaa tcgggctcat gagcatctca tcgtgatggg aaaggcgtcg
     1021 atcgccttgc caagtgaacc acacaacagc tcccgtgtca aatggatggg gagcggcacg
     1081 ttagccctgc cgacacctgt atccctcgtc cacgagggag gctgctcggg tggggtcaca
     1141 ggcggcggga tcgcccgggc agggaaacgg ggaacgactc ggtctccatg cagagccctc
     1201 gagggctggc tcccttcacc ggcaggtcag cgccgggaag g
//
LOCUS       DAOSCR010000733         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_75531_length_1239_cov_3.013514, whole genome shotgun sequence.
ACCESSION   DAOSCR010000733 DAOSCR010000000
VERSION     DAOSCR010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75531_length_1239_cov_3.013514"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..>1239
                     /locus_tag="PLT69_13170"
     CDS             168..>1239
                     /locus_tag="PLT69_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ15437.1"
                     /translation="MDDDLLSELREFEMLYRTSRMDVLRTVIDQEAESEGVDRVFIMV
                     TSPGKKILASSNMNSWKDIPTSTSAPTSMEGVHYESFSSKTHPDGVRMAHRALGDGNV
                     ITIGMDPKGDRAVLNAFAEVSLTAILFVLITGTLTGWYISKKAMLGVERVRLTASGID
                     SRNISSRVPLGHEGEEIKNLASTFNSMLDRIESLITELKEVTTNIAHELRSPLTRIRS
                     MAESTLTEYTDRSRRQEAASLILEECDHMAGIINTMLEIAQTDAGILRIARERVDIGD
                     LIRKAHELFLVVAEDNGISFDIEIPPEDLFIEGDTVRLQRCISNLLDNAFKFTPRGGR
                     VTISARRLSDQVEVVVADTGEGI"
BASE COUNT          285 a          357 c          358 g          239 t
ORIGIN      
        1 gatccacacc atccgcgggg cagggtacat ccttgaagaa agggagtaga ctcttcagca
       61 ccgtatcctc gcggcttgcc ctgtggtatg ccgtgctgtt cacagcgtcg ggcctcttga
      121 gcttcatgat cgtctatgtc atgctatctt tgacccttga ggagcgcgtg gacgatgacc
      181 tgctctcaga gctcagggag ttcgagatgc tctaccgcac aagcaggatg gatgtcctca
      241 ggacggtcat cgaccaggag gccgaatcgg aaggcgtcga ccgtgtgttc atcatggtca
      301 cctcgcccgg gaagaagatc ctcgcctcgt cgaatatgaa cagctggaag gacatcccca
      361 cgtccacaag cgccccgacc tccatggaag gtgtgcatta cgagtcattc tcctcgaaga
      421 cccacccgga tggagtcagg atggcccacc gggccttggg agatggcaac gtcataacca
      481 tcggcatgga cccgaaaggc gacagggccg tgctcaatgc attcgccgag gtgtcgctga
      541 cggcgatact cttcgtcctg atcacaggca ccctcacggg atggtatatc tcgaaaaagg
      601 ccatgctcgg tgtggaaagg gtacggttga cggcctcggg catagactcg aggaacatat
      661 cttcacgcgt accccttggc catgaaggag aagagatcaa aaatcttgca tccaccttca
      721 actccatgct cgaccggatc gagtccctca tcaccgagct gaaggaggtg accaccaata
      781 tcgcccatga gctgaggagc cccctgacga ggatacggag catggcagaa tccacgctca
      841 cggagtatac ggacagatcc cgtcgccagg aagccgcctc cctgattctc gaggaatgcg
      901 atcacatggc gggcatcatc aacaccatgc tcgagatagc acagaccgat gcgggtatcc
      961 tccggattgc cagggaacgg gtcgatattg gcgatctcat ccgaaaggca cacgagctct
     1021 tcctcgtcgt tgccgaagac aacgggatat cattcgacat cgagatccca cccgaggacc
     1081 tgttcatcga gggagatacc gtgcgtctac agcggtgcat ctccaatctt ctggacaacg
     1141 ccttcaagtt tacgccccgg ggtggcaggg tgacgatatc cgcgcggaga ctgtcggacc
     1201 aggtggaagt ggtcgtggca gatacggggg aaggcatcg
//
LOCUS       DAOSCR010000734         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_75692_length_1237_cov_3.043147, whole genome shotgun sequence.
ACCESSION   DAOSCR010000734 DAOSCR010000000
VERSION     DAOSCR010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75692_length_1237_cov_3.043147"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLT69_13175"
     CDS             complement(<1..439)
                     /locus_tag="PLT69_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810907.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOJ15438.1"
                     /translation="MNQIDLMKELHVQSDKKIVMLVLDGLGGLPGPSGKTELEEARTP
                     NMDRLCRQAVCGLSTPITPGITPGSGPGHLGLFGYDPLEYTIGRGVLEALGIGLTLRP
                     TSIAARGNFCTLDPQTGVITDRRAGRIPTSKCAELVAKLRAITL"
     gene            complement(682..>1237)
                     /locus_tag="PLT69_13180"
     CDS             complement(682..>1237)
                     /locus_tag="PLT69_13180"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOJ15439.1"
                     /translation="VSVKMRIGCSPDPEAMCDFASMLEDAGADFIVLHARTAADGLSR
                     KARWEYIARLKERLRVPVVGNGGVTRAEDALEMFSSTGCDGVMVGRQAVIQPWIFRDI
                     KALMEGSQPEPPPPLEDVILDLHRLLLEHFGEDVALKRFKTGVVWLSQNLTFGHHLTT
                     LVGRQKTMEGAASVVRDAFLKGIS"
BASE COUNT          204 a          400 c          412 g          221 t
ORIGIN      
        1 ccagggtgat ggccctgagc ttcgccacga gctccgcgca cttcgatgtg gggatcctgc
       61 ccgcacggcg gtcggtgatc acccccgtct gagggtcgag ggtgcagaag ttcccccttg
      121 ccgctatgct ggtcggcctg agggtgagcc ctatgccgag cgcctcgagc acccccctgc
      181 cgatcgtgta ctcgagggga tcgtaaccga agaggcccag gtggccgggc ccgctcccag
      241 gggtgatccc gggggtgatg ggcgtggaca gaccgcacac ggcctgcctg cagagcctgt
      301 ccatgttggg agtgcgggcc tcctcgagct cggtcttgcc ggaagggccc ggcaggcccc
      361 cgaggccgtc caggacgagc atgacgatct tcttgtcgga ctgtacgtgg agctccttca
      421 tcaggtcgat ctggttcatg agcctctcct taggaagcag gatgggtcga tcgccgctct
      481 ggtgcgttct cgatcaagaa cggttataca acctccccgg aaaaattcaa ggaccaacga
      541 gacggcgccg agccgcctcc atcgagaggg cacggaaggc gtcgcccgtg gggaaagggt
      601 gcatggccgg ggcgtcgagc tccccagggg gtcgtgagcc tgcctgcgcc ggcacgtgag
      661 gttgagtgag cgtacccgga ctcagctgat gcccttgagg aatgcgtccc tgacgaccga
      721 ggccgcacct tccatggtct tctgcctccc gacgagcgtg gtgaggtggt ggccgaaggt
      781 gaggttctga gacagccaca cgacgccggt cttgaagcgc ttgagggcca cgtcctcgcc
      841 aaagtgctcg aggagaaggc ggtgcaggtc caggatcacg tcttccagcg gcgggggagg
      901 ttcgggttgc gagccttcca tgagcgcctt gatgtccctg aagatccatg gctgtatcac
      961 cgcctgcctg cccaccatga cgccgtcgca cccggtcgag ctgaacatct cgagggcgtc
     1021 ttcggcgcgc gtgacgccgc cgtttcccac cacgggcacg cgtaagcgct ccttgagcct
     1081 ggctatgtat tcccacctcg ccttcctcga cagcccgtcg gccgcggtcc tcgcgtggag
     1141 cacgatgaaa tccgcacccg catcctcgag catcgaggcg aagtcgcaca tggcctcggg
     1201 atccggggag cagccgatgc gcatcttcac cgatacc
//
LOCUS       DAOSCR010000735         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_75864_length_1235_cov_3.117797, whole genome shotgun sequence.
ACCESSION   DAOSCR010000735 DAOSCR010000000
VERSION     DAOSCR010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75864_length_1235_cov_3.117797"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="glnS"
                     /locus_tag="PLT69_13185"
     CDS             complement(<1..1023)
                     /gene="glnS"
                     /locus_tag="PLT69_13185"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOJ15440.1"
                     /translation="MAGPTKNAPPSGATDPSRPVDFIRAIINDHMRTGRFEGKVHTRF
                     PPEPNGYLHIGHAKSIFLNFGIAQEYEGLCNLRFDDTNPSKEDQEYIDSIIEDVRWLG
                     MDWGDRMFFASDYFEKTYELAEELIKKGLAYVCDLTPEEVRQYRGTLTEPGRESPYRN
                     RSVEENLELFRRMRAGEFEDGSRTLRAKIDMASPNMNMRDPVIYRILRATHHRTGDDW
                     CIYPMYDFAHPISDSLEGITHSICTLEFEDHRPLYDWFLEKLGMFHSQQIEFARLNLS
                     YTLMSKRRLLELVELGVVEGWDDPRMPTIAGLRRRGYTPEAIRKFCDRIGVSKKDSMV
                     DLALLEH"
BASE COUNT          188 a          380 c          400 g          267 t
ORIGIN      
        1 gtgctcgagc agcgccaggt ccaccatgct gtctttcttc gacacgccga tgcgatcgca
       61 gaatttcctg atggcctctg gcgtgtaccc cctgcggcgc agcccggcga tggtcggcat
      121 gcgcggatcg tcccagccct ccaccacgcc cagttccacg agctccagga gcctccgctt
      181 gctcatgagg gtgtagctga ggttcagcct cgcgaactcg atctgctggg agtggaacat
      241 gccgagcttc tccaggaacc agtcgtacag cgggcggtga tcctcgaact cgagcgtgca
      301 gatggaatgg gtgatgcctt cgagggagtc ggagatgggg tgggcgaagt cgtacatggg
      361 gtagatacac cagtcgtcgc ccgtccggtg gtgcgtcgcc ctgaggatcc tgtagatcac
      421 ggggtccctc atgttcatgt tgggagaggc catgtctatc ttcgccctga gcgtcctcga
      481 gccgtcttcg aactcgccgg ccctcatccg cctgaacagc tcgagattct cctccacgct
      541 gcggttgcga tacgggctct ccctgcccgg ctcggtgagg gtcccccggt actgcctgac
      601 ctcctccggg gtgaggtcgc acacgtaggc gagccccttt tttatgagct cctcggccag
      661 ctcgtaggtc ttctcgaagt agtccgaggc gaagaacatc cggtcccccc agtccatccc
      721 gagccacctc acgtcctcga tgatggagtc gatgtactcc tggtcctcct tggaggggtt
      781 cgtgtcgtcg aagcgcaggt tgcacaggcc ttcgtactcc tgcgcgatgc cgaagttcag
      841 gaagatcgac ttggcgtggc ctatgtgcag gtagccgttc ggctccggcg ggaagcgcgt
      901 gtggacctta ccctcgaacc tcccggtcct catgtggtcg ttgatgatgg ccctgatgaa
      961 gtcaacgggc ctggacgggt ctgttgcgcc tgaaggtggt gcgttcttcg tcggtcctgc
     1021 catggacggg tacctcggca ctcacgttga gttctatgtc ttataccaca gggacgaggc
     1081 gttttgaacg gctgaacgca tggtccaagc ccgtgacggg cggcggtgcc tcacctccgc
     1141 cgcgagcggc ggcgtcgtgc aagccggcgt cttgggcggc gtatggggcg tgacgagcgg
     1201 cttcgtgaac ggggacgtcc tctgcgggct cttgg
//
LOCUS       DAOSCR010000736         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_75867_length_1235_cov_2.795763, whole genome shotgun sequence.
ACCESSION   DAOSCR010000736 DAOSCR010000000
VERSION     DAOSCR010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75867_length_1235_cov_2.795763"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLT69_13190"
     CDS             complement(<1..950)
                     /locus_tag="PLT69_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOJ15441.1"
                     /translation="MDVFIDYPKRLEKIRGQMRASGIDLLIGTRTVSLSYVAGAFVPW
                     RSTVLVSTDGYASLTTLLIDLERMKHESWLSNIHGYAPLPGMDLWDVTVHQIRSLNLD
                     KARIGVEVGHSPRGNTGYLFSTEFDLLRQALPDAALVPANHVMDRASYVKEPGEIALM
                     RQAAAMADSAVERVKEALCVGMTEAEIAGIGEMELRRLGSSYHWAVTGSSEVASGERA
                     CWAMNGTTPPSTKLVQKGENIIVDFHPCCNLYYSDLAHNFIIGKPNAEQARLADAFAR
                     TAETLVNALKAGSTVGQVFRAVHDTVSGLGYAGFTIPSFG"
BASE COUNT          204 a          401 c          403 g          227 t
ORIGIN      
        1 tagccgaagg acgggatcgt gaaccctgca tacccgagcc cgctcaccgt gtcgtgcacc
       61 gcccggaaga cctgccccac cgtgctgccg gccttcaggg cgttgacgag ggtctcggcc
      121 gtccgtgcga aggcatcggc cagcctcgcc tgctctgcgt tcggcttccc gatgatgaag
      181 ttgtgcgcca ggtccgagta gtagaggttg cagcagggat gaaagtccac gatgatgttc
      241 tcgcctttct ggacgagctt ggtgctgggc ggggtggtgc cgttcatggc ccagcaggcg
      301 cgttcgccgg atgccacctc cgaggacccg gtcacggccc agtggtatga actccccagg
      361 cgcctgagct ccatctcgcc gatgccggcg atctcggcct cggtcatgcc cacgcacagt
      421 gcctccttca cccgctcgac cgcgctgtcc gccatggcgg cggcctgacg catgagcgct
      481 atctcgcccg gctccttgac gtacgaggcc ctgtccatca cgtggttggc cgggacgagg
      541 gcggcgtctg gcagggcctg cctgagcagg tcgaactccg tggagaagag gtagcccgtg
      601 ttgccgcggg gcgagtgacc gacctccacc cctatcctgg ctttatcgag gttcaggctc
      661 ctgatctggt gcacggtgac gtcccagagg tccatgcccg gcagcggggc gtagccgtgg
      721 atgtttgaga gccaggactc gtgtttcatc cgttccaggt cgatgagcag cgtggtgagc
      781 gacgcgtacc cgtccgtcga gacgagcacg gtgctcctcc aggggacgaa ggccccggcc
      841 acgtagctca ggctcacggt gcgcgtgccg atgaggaggt ctatgccgct tgcgcgcatc
      901 tggccgcgga tcttctcgag gcgtttgggg taatcgatga acacgtccat ggggtcctcc
      961 tttctcgtgt gtcatgggtg cgcacggttg gtgccgaccg gcgggcgcca cgcgcctcga
     1021 tgcccacgag gccccggcgc atgagaagcc ctgcgtgctg ccggccccct gcacgcccac
     1081 cataccatcc agggccgggc cccgtcatcc atgatcctgg acacgtgcga acgctctatg
     1141 atacatgctc tcgaatccag aaaatacacc atgtaatggg aggacgacac atgctgacca
     1201 agagcggaaa gagcctcgaa gagctgatca agaag
//
LOCUS       DAOSCR010000737         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_76129_length_1232_cov_3.933730, whole genome shotgun sequence.
ACCESSION   DAOSCR010000737 DAOSCR010000000
VERSION     DAOSCR010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76129_length_1232_cov_3.933730"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1232
                     /gene="bamA"
                     /locus_tag="PLT69_13195"
     CDS             <1..>1232
                     /gene="bamA"
                     /locus_tag="PLT69_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOJ15442.1"
                     /translation="YYGVEVSTSTTPTEGGVVLTFTVVENEQLYIRGISFDGNENIKA
                     GKLSGVMETKTRWPLGIFGHEGAYDSALLDNDLLRIEQYYADNGYMQAKVGRPRVDIR
                     EKDGIYITIPVSEGPLFTVGAVEIEGDLVEPKERLMEKVALSTGSPMSKSALQQSVEN
                     LRSVYMDKGYAYAKIRPVTEEEGENRLAVTFRIDKGKPVVVENIQIRGNTKTRDKVIR
                     RELEIEESKTFSSTAIKDSKDNLSRLGYFSTVNIDPVPKSDSSMDLLVDVQETTTGAF
                     SFGFAYSSSDGPMGTTSLSENNFFGLGYKVKGSIDYGPKRKSYSIDAEDPWFLDYNLS
                     LGGRVYNLEREYIYYTKRSVGGNIRLSYPLFERVRHSVIYAYENVHELDDIDPAYLQS
                     LSEEEIEGGVTSSVINTI"
BASE COUNT          287 a          381 c          377 g          187 t
ORIGIN      
        1 atactacggt gtcgaggtga gcacctccac gacaccgacc gagggagggg tcgtcctcac
       61 gttcacggtg gtagagaacg agcagctcta cataaggggc atcagcttcg acggaaacga
      121 gaacatcaag gcagggaaac tctcgggcgt catggagacc aagacgaggt ggccgctcgg
      181 catcttcggg cacgaggggg cctacgacag cgcgctgctc gacaacgacc tcctgcgcat
      241 cgagcagtat tacgcggaca acgggtacat gcaggccaag gtgggcaggc ccagggtcga
      301 catcagggaa aaggacggca tctacatcac gatcccggtg tccgagggcc cgctcttcac
      361 cgtgggtgcc gtggagatcg agggcgacct cgtcgagccc aaggagaggc tcatggagaa
      421 ggtggccctg agcacgggga gccccatgag caagagcgcg ctccagcagt ccgtcgagaa
      481 cctgcgctcc gtttacatgg acaagggtta cgcctatgcg aagatcaggc cggtgaccga
      541 agaggagggg gaaaaccgcc tggccgtcac cttccgcatc gacaagggca agccggtggt
      601 cgtggaaaac atccagatca ggggcaacac caagaccagg gacaaggtca tcaggcgcga
      661 gctggagatc gaggaatcga agaccttctc ctccaccgct atcaaggaca gcaaggacaa
      721 cctctcgcgc ctgggctact tcagcacggt gaacatcgac cccgtaccca agagcgactc
      781 gtccatggac ctcctggtcg acgtccagga gaccaccacg ggcgcgttct ccttcgggtt
      841 cgcctactcc tcctcggacg ggcccatggg cacgacgagc ctgagcgaga acaacttctt
      901 cgggctcggc tacaaggtga aaggcagcat agactacggt cccaagcgga agagctactc
      961 catagacgcg gaggacccct ggttcttgga ctacaacctg tccctcggcg gccgcgtgta
     1021 taacctcgag cgcgagtaca tctactacac gaagcggtct gtcggcggaa acatccggct
     1081 gagctacccc ctcttcgagc gggtccgcca ctccgtcatc tacgcgtacg agaacgtcca
     1141 cgaactcgac gacatcgacc cggcctacct ccagtcactg tccgaggagg agatcgaggg
     1201 cggggtcacc agcagcgtca tcaacaccat ct
//
LOCUS       DAOSCR010000738         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_76249_length_1231_cov_2.386905, whole genome shotgun sequence.
ACCESSION   DAOSCR010000738 DAOSCR010000000
VERSION     DAOSCR010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76249_length_1231_cov_2.386905"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(494..1183)
                     /locus_tag="PLT69_13200"
     CDS             complement(494..1183)
                     /locus_tag="PLT69_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ15443.1"
                     /translation="MNVTWRILIIEDDQPLALGLRINLESEGYEVMHALSAEEGLRIL
                     DNSSVDLVILDLMLPGIDGIEALQRIRQSDVKLPVLVLTARSGIDDKVEGLRSGADDY
                     LTKPFHLEEFLLRVKRMLDRYQWYKSEEVLVIGDRKVDFNTLEITGPSGVNHRITPHE
                     ANLIQYLASHQSRIVTRSELLKNVWGLDPDLETRTVDTFVSRIRKYLEDDPGNPRHIV
                     SIRGKGYRFVP"
BASE COUNT          195 a          376 c          404 g          256 t
ORIGIN      
        1 ttcctggtag gccttcatgc cgagggcggc ctcctcgctc tccgacacga ggagcaactg
       61 cttcctcccc gtgtagggcg aggtggcgca ccctgcgatg aaggcgcacg tggcgaggat
      121 cagaacgaag acgccgggcg catgaacgcg ggctttctct ctcatggtca tgacctcctg
      181 tgacctgttg tgtgggcatg catccttgcg gttgatgaat gtgatgttaa ggcaaacgcc
      241 ctgccggttg caaggacgag gcaggttcgt gtgcaacctc ctcgggggtt gcccgtgagg
      301 gtctcttcgg ctcctctcga gcctccaggc gtcggccggg tgcatgagcg cctcatgcct
      361 gcacggggtt cgtggagctg gcccgcacga gacggaggcc ttcctcggcg gccgccggat
      421 gaaagcctgg agcccctcgt acgatgctcg cgtaccgggc gggcacaggg tggaacaccc
      481 gggccatgct gattcatggg acgaaccggt atcccttccc cctgatgctc acgatgtgcc
      541 tgggattccc ggggtcatcc tcgaggtact tcctgatgcg cgagacgaag gtgtccacgg
      601 tcctcgtctc gagatcgggg tccaggcccc acacgttctt cagcagctcc gaccgggtca
      661 cgatcctcga ctggtgggag gccaggtatt ggatgaggtt cgcctcgtgc ggggtgatcc
      721 tgtggttgac tcccgagggc ccggtgatct cgagggtgtt gaagtccacc ttcctgtccc
      781 cgataacgag gacttcctcc gacttgtacc actggtagcg gtcgagcatc cttttgaccc
      841 tgaggagaaa ctcttccagg tggaacggct tggtgaggta gtcgtccgca cccgagcgga
      901 gcccctcgac cttgtcgtcg atgccggagc gagcggtcag cacgaggacc gggagcttca
      961 cgtccgactg ccggatccgc tgcagggcct ctatgccgtc gatgccgggc agcatgaggt
     1021 cgaggatcac gagatccacg ctcgagttgt cgaggatcct cagcccttcc tccgcgctca
     1081 gcgcgtgcat gacctcgtag ccctccgact cgaggttgat cctcaagccg agggccaggg
     1141 gctggtcgtc ctcgatgatg agaatgcgcc aggttacgtt catgtcttgg cgagtggcag
     1201 ccgtatgctg aacgtgctgc cgtgttctct g
//
LOCUS       DAOSCR010000739         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_76336_length_1230_cov_2.658723, whole genome shotgun sequence.
ACCESSION   DAOSCR010000739 DAOSCR010000000
VERSION     DAOSCR010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76336_length_1230_cov_2.658723"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..981
                     /locus_tag="PLT69_13205"
     CDS             514..981
                     /locus_tag="PLT69_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15444.1"
                     /translation="MMRGFTMVVLGLAVAGLLLGAGASTVSAGQAGKAPTGGMGERRE
                     ILDALGRRVRDLHGLDVVFSAASLKVKDGWAWVFACPTSKDGANRYEPVAALLRMQGG
                     AWKVVELPCLEEDNPDCLTAPTYLSSLKRRFPGLPAEILPRDAEKELGSVCTP"
BASE COUNT          195 a          387 c          449 g          199 t
ORIGIN      
        1 gctgcgctca cccatgggag gcacaacatc cggcacccag gcgcggagtc attcgtgcac
       61 gccaggggtg cacccggcaa accgccgggg agtgggctgc ccaggacctg ccggtcgaag
      121 gcccccggcc tcacaggctc cacgcatggc ctcggccgaa gtccgcctga gcccaggggc
      181 ctcttcgatc caccctgaac gcacgggcgg gcacgtggtc ttcgatgggg aatgcacccg
      241 tgtcgtaggg ggaaacgcct cgtgaggatc gagggggtag gccttcggca tgaccttcac
      301 cttttcgtgt tgcccgccgc atcgttcgaa cggtccttcg gtgtccagga agcaactggc
      361 cgaatcgatc ccgattcatc ccgtctcgcg cctggcggcc cgtgcatggg cagaaccccg
      421 tcccgaagga tgaacgcgcc gttgtcgccg tatacgcacc cttgcatgga acgggctgag
      481 agcctatcaa tcggtctcat ggaggtgcgt cgcatgatga gaggtttcac gatggtcgtg
      541 ctcgggcttg ccgtggcggg tctcctgctc ggtgcagggg cgtccacggt atcggcagga
      601 caagcgggga aagcgcctac gggcggcatg ggcgagagga gggagatcct ggatgccctc
      661 gggaggcggg tcagggatct ccacgggctc gatgtggtct tcagcgccgc cagcctgaag
      721 gtgaaggacg gttgggcatg ggtgttcgcc tgcccgacgt ccaaagacgg cgcgaacagg
      781 tacgagcctg tggcagcctt gctccgcatg cagggcggtg catggaaggt ggtggaactg
      841 ccctgcctcg aggaggacaa ccccgactgc ctgactgcac cgacgtacct ctcctcgctc
      901 aagaggagat ttccgggcct gcccgccgag atactcccga gggatgcgga gaaggagctc
      961 gggtctgtct gcacgccttg acgaggacgg gccgctgtcg gaggggcggg gacgcgaagc
     1021 cggatcccgc ccttgccctg ctggatgtgt ccgcggggga tgtggcgggc gagaacgccc
     1081 cgtggagcgc cttcccgcgg ggcccctgac cacgtgggaa ggcggcggag gcgcgtgagc
     1141 agcccaacgg gccgcttccc gggccggggc cgtcgcaggc gggcctgccg gtatcgacct
     1201 gcggtgcgcg tcacggcctt ggtgaccatg
//
LOCUS       DAOSCR010000740         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_76925_length_1224_cov_2.256630, whole genome shotgun sequence.
ACCESSION   DAOSCR010000740 DAOSCR010000000
VERSION     DAOSCR010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76925_length_1224_cov_2.256630"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..145)
                     /locus_tag="PLT69_13210"
                     /pseudo
     CDS             complement(1..145)
                     /locus_tag="PLT69_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            80..337
                     /locus_tag="PLT69_13215"
     CDS             80..337
                     /locus_tag="PLT69_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15445.1"
                     /translation="MDGEKHLQDLAVADDGGIELQAGSKVTITSGGWVVTVDASGQIT
                     ASGTSITLNAGGSSVEIGASGISLTTGGMITLTGSMIKNNC"
     gene            348..758
                     /locus_tag="PLT69_13220"
     CDS             348..758
                     /locus_tag="PLT69_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15446.1"
                     /translation="MAPRKKASAMARVIDLEEGRRLVLTREGNSDRITVTGPDGGVRL
                     TITLTDRGPVLHLEGACITIETDGDLSISAGSLALNARDGISLSTGGDAAVKVEGDLS
                     IRARTHTIEAELGDVNVRANDDVRIEGERILMNC"
     gene            complement(773..>1224)
                     /locus_tag="PLT69_13225"
     CDS             complement(773..>1224)
                     /locus_tag="PLT69_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ15447.1"
                     /translation="PAGEAPRVGKVQVPLIVQYGPSVRTFREVPVTIGSAPDADLVMA
                     HAGMAPRHVMIFSSGGAYWVKDLTGRGMVTINGRPVVTEAQMNSMDLVSLGPGLPTFK
                     YLGEGRLAEIEQEPAHKPETREAHTQPQDAPKPVKKPQKSIFDIFKR"
BASE COUNT          217 a          372 c          439 g          196 t
ORIGIN      
        1 gctcgtccga gtgcgtgaag atcgccgagc ggtactgcac gccgatatcg ttgccctggc
       61 ggtcgcgcga ggtggggtcg tggatggcga aaaacacctc caggatctcg cggtagctga
      121 cgatggcggg atcgaactcc aggcgggcag caaggtcacc atcaccagcg gtgggtgggt
      181 cgtcacggtg gacgcctccg ggcagataac cgcgagcggc acctccatca ccctgaacgc
      241 gggcggctcc tcggtggaga tcggcgcttc cggcatcagc ctcaccacgg gagggatgat
      301 cacgctcacc gggtccatga tcaagaacaa ctgttgagga gggtacgatg gccccacgaa
      361 agaaggcgtc ggccatggcc agggtgatag acctcgagga aggtcgtcgg ctcgtcctga
      421 ccagggaggg aaactcggac aggatcaccg tgaccgggcc tgacggcggg gtgagactca
      481 cgatcaccct gaccgacagg gggcctgtcc tccacctcga gggtgcctgc atcacgatcg
      541 agaccgacgg cgatctctcc atcagcgccg gcagcctcgc cctgaacgca cgcgatggga
      601 tctccctgag cacgggaggg gacgcggcgg tgaaggtgga aggcgacctg tccatcaggg
      661 cgaggacgca caccatcgag gccgagctcg gcgatgtgaa cgtccgggcg aacgacgacg
      721 tgaggatcga gggcgagagg atcctcatga actgttgagg ggcaagggcg tgtcagcgct
      781 tgaatatgtc gaagatggac ttctggggtt tcttgacggg cttgggcgcg tcttgcggct
      841 gcgtgtgcgc ctctctcgtc tccggtttgt gggcgggctc ctgctcgatc tccgcgagcc
      901 tcccctcgcc gaggtacttg aaggtgggga ggccggggcc gagggacacg aggtccatgg
      961 agttcatctg cgcctcggtg acgaccggcc tgccgttgat ggtcaccatg cccctgcccg
     1021 tgaggtcctt gacccagtag gcgcccccgc tcgagaagat catgacatgg cgcggggcca
     1081 tgcccgcgtg ggccatgacg aggtccgcat ccggcgcgga gccgatcgtg acgggcacct
     1141 ccctgaacgt gcggaccgac ggcccgtact ggacgatgag ggggacctgg accttgccga
     1201 cccggggcgc ctcacctgca ggcg
//
LOCUS       DAOSCR010000741         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_77026_length_1223_cov_2.321062, whole genome shotgun sequence.
ACCESSION   DAOSCR010000741 DAOSCR010000000
VERSION     DAOSCR010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77026_length_1223_cov_2.321062"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLT69_13230"
     CDS             complement(<1..819)
                     /locus_tag="PLT69_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOJ15448.1"
                     /translation="MPAQRPEIWVFGDYRNYFQNRVTLQLLSRATDLAEKRGALVCAT
                     VFGWDTGEWVGEYIAHGARKVYLVEDRRLGRYSCDLYVGLMERLARRYRPEIILVGAT
                     SFGREFAPRVAKRLKTGLTADCVGLDIDENGLLVQTAPAFGGNLLTRIVTPQKRPQMA
                     TVRPGVFREIPHDHGATGEIVRIDFPEDMPPDRVRLVSSERTLPVPRHLGNAEIVVCG
                     GRGMGSKGRFRNLYELARLLGGEVGATRPAVYARWADEDALVGQADKHIRPKVLF"
     gene            complement(825..>1223)
                     /locus_tag="PLT69_13235"
     CDS             complement(825..>1223)
                     /locus_tag="PLT69_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15449.1"
                     /translation="AYVDEVTVEGGTARLRRVSDGFLETLEIDLPCLVTVNTTNRSPR
                     YVPLSGLEDAFGEVNILSVGVADLGIDPALVGADGSPTRIVDVTAPRSGKENVVLTGA
                     PKKIVEELLERFESRISGLIRQDIRKSDAR"
BASE COUNT          184 a          441 c          382 g          216 t
ORIGIN      
        1 gaagaggacc ttgggccgta tgtgcttgtc cgcctgcccg acgagggcat cctcatccgc
       61 ccaccgggca tacaccgccg gtctcgtggc gccgacctcc ccgcccagga gcctggcaag
      121 ctcgtagagg ttcctgaacc tccccttgct ccccatgccc ctgcccccgc acacgacgat
      181 ctccgcgttc cccaggtgcc tcggcaccgg cagggtgcgc tcggaggaga cgagcctcac
      241 cctgtccggg ggcatgtcct cagggaaatc gatgcggacg atctccccgg tggcgccgtg
      301 atcgtggggg atctccctga acaccccagg cctgacggtc gccatctggg gccgcttctg
      361 cggtgtcacg atcctcgtga ggagattgcc gccgaaggcc ggtgcggtct gcacgagcag
      421 gccgttctcg tcgatgtcca ggccgacgca gtcggccgtg agccccgtct tgagcctctt
      481 cgccaccctc ggcgcgaact ccctgccgaa ggaggtggcg cccaccagga tgatctcggg
      541 acggtaacgc ctggcgaggc gttccatgag gccgacgtag aggtcgcagg aataccggcc
      601 gaggcgcctg tcctccacga ggtagacctt cctcgcgccg tgggcgatgt actccccgac
      661 ccactcgcct gtgtcccagc cgaacacggt ggcgcacacg agagcgcccc tcttctcggc
      721 gaggtcggtc gcccgcgaga ggagctgcag ggtgaccctg ttctggaaat agttccggta
      781 gtcaccgaag acccagattt ccggccgctg tgctggcatg gccctcacct cgcgtcgctc
      841 ttcctgatgt cctgcctgat gagcccgctg atcctggact cgaaccgctc gaggagttcc
      901 tccacgatct tcttgggcgc acccgtgagc accacgtttt ccttgccgga cctcggcgcc
      961 gtcacgtcca cgatcctcgt gggcgaaccg tccgccccga cgagcgcagg gtcgatcccc
     1021 aggtccgcaa cgcccacgct caggatgttc acctctccga aggcgtcctc gaggccggag
     1081 agcgggacgt acctggggct cctgttcgtg gtgttgaccg tgaccaggca cgggagatcg
     1141 atctcgaggg tctcgaggaa gccgtccgag acgcgcctga gcctggcagt gcccccttcc
     1201 accgtgacct cgtcgacgta cgc
//
LOCUS       DAOSCR010000742         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_78699_length_1204_cov_3.904265, whole genome shotgun sequence.
ACCESSION   DAOSCR010000742 DAOSCR010000000
VERSION     DAOSCR010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78699_length_1204_cov_3.904265"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /gene="tuf"
                     /locus_tag="PLT69_13240"
     CDS             <1..182
                     /gene="tuf"
                     /locus_tag="PLT69_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOJ15450.1"
                     /translation="TDVTGDIKLPEGVEMVMPGDNVTMEVKLISPIAMEEGLRFAIRE
                     GGRTVASGVIVKVIE"
     gene            213..521
                     /gene="rpsJ"
                     /locus_tag="PLT69_13245"
     CDS             213..521
                     /gene="rpsJ"
                     /locus_tag="PLT69_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOJ15451.1"
                     /translation="METQRIRIRLKGYDHKLIDQSVQEIVDAARKTGAKVAGPIPLPT
                     KIERFCVLRSPHVDKKSRDQFEIRTFKRLLDILDPTQQTVDSLMKLDLSAGVDVEIKL
                     "
     gene            540..1169
                     /gene="rplC"
                     /locus_tag="PLT69_13250"
     CDS             540..1169
                     /gene="rplC"
                     /locus_tag="PLT69_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOJ15452.1"
                     /translation="MIKGILGKKLGMSQVFNENGDIVPVTLIQAGPCFVIQKKVKDRD
                     GYDALQLGFGPKKESRVNKPLKGHQDKAGKGYFSFLREIPCDDVSSVEVGAEVKVTDV
                     FSKGEVIKVTGVSKGKGYAGVVRRHGFGGLPASHGSLILNESGSIGTNTTPGKVIKGK
                     RMAGHLGNRRVTVKGLKVVDIIEDMNMIAVRGAVPGSKGQLVILRKQEG"
BASE COUNT          284 a          334 c          400 g          186 t
ORIGIN      
        1 ctacggacgt gacgggggac atcaagctgc ccgagggggt ggagatggtg atgcccgggg
       61 acaacgtgac gatggaggtg aagctgattt cgccgatcgc gatggaggag ggtttgcgat
      121 tcgccatccg cgagggcggc aggaccgtgg cctccggcgt catcgtcaag gttatcgagt
      181 aagctcagag aaacgacagg agaataccgg caatggagac ccaacggata cgcatacgac
      241 tgaaaggcta cgaccacaag ctcatcgacc agtccgtcca ggagatcgtc gatgcggcac
      301 gcaagaccgg cgccaaggtg gccgggccca tcccccttcc gacgaagatc gagcgtttct
      361 gcgtcctgag gtctccccac gtggacaaga agtcgagaga ccagttcgag atccgcacgt
      421 tcaagcgcct cctcgacatc ctcgacccga cccagcagac ggtcgactcc ctcatgaagc
      481 tcgacctgtc ggccggcgtc gacgtggaga tcaagctcta gcacgaagcg gagaaagcca
      541 tgatcaaggg tatactcggc aagaagctcg gaatgagcca ggtgttcaac gaaaacgggg
      601 acatcgtccc ggtgacgctc atacaggcgg gtccctgctt cgtgatacag aagaaggtga
      661 aggacagaga cggctacgac gccttgcagc tgggattcgg accgaagaag gaatcccggg
      721 tcaacaagcc gctcaaggga caccaggaca aggccggcaa gggatacttc tcctttctcc
      781 gcgagatccc gtgcgacgac gtctcctcgg ttgaagtcgg cgcggaggtg aaggtgaccg
      841 acgtgttcag caagggcgag gtcatcaagg tcaccggtgt ctccaaggga aagggatacg
      901 cgggcgtggt gaggaggcac gggttcggag ggctgccggc gagccacggg tccctcatcc
      961 tcaacgagag cgggtccatc ggcacgaaca cgaccccggg caaggtcatc aagggcaagc
     1021 gcatggccgg acacctggga aaccgcaggg tgaccgtcaa gggcctcaag gtcgttgaca
     1081 tcatcgagga catgaacatg atcgccgtgc gcggggcggt tcccgggagc aagggacagc
     1141 tggtcatcct gcgcaagcaa gagggttgat acgatgcctg tggtcgacgt ttacaacata
     1201 gctg
//
LOCUS       DAOSCR010000743         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_78841_length_1203_cov_2.076655, whole genome shotgun sequence.
ACCESSION   DAOSCR010000743 DAOSCR010000000
VERSION     DAOSCR010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78841_length_1203_cov_2.076655"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLT69_13255"
     CDS             complement(<1..287)
                     /locus_tag="PLT69_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ15453.1"
                     /translation="MPVTVEPWHWLVFGMALLIAELFAGSMVLLWFGLSALVIAILVF
                     LAPALGLTWQLLAWAVCSGLLTVLWFLFLRPKMADRTKAGAGYEALVGETG"
     gene            complement(466..864)
                     /gene="mscL"
                     /locus_tag="PLT69_13260"
     CDS             complement(466..864)
                     /gene="mscL"
                     /locus_tag="PLT69_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005298202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOJ15454.1"
                     /translation="MGMIKEFKEFAMRGNVVDMAVGIIIGAAFGKIVSSLVADVIMPP
                     IGVLLGGVDFTDLAITLKAASDQAAAVTLNYGKFIQTVVDFLIIAFAIFLLVKGINAF
                     KRKEEEKPAEPTKEVVLLTEIRDLLGQQRK"
BASE COUNT          211 a          392 c          392 g          208 t
ORIGIN      
        1 atgcccgtct cgcccaccag cgcctcgtaa cctgccccgg ccttcgtcct gtccgccatc
       61 ttgggcctca ggaagaggaa ccagagcacg gtgagcaggc ccgagcacac cgcccaggcc
      121 agaagctgcc acgtgagccc caaagccggc gccaggaaga cgaggatcgc gatcacaagg
      181 gctgacaggc cgaaccagag gagcaccatg ctccccgcga agagctccgc gatgagcagg
      241 gccatgccga agacgagcca atgccatggt tccacggtta cgggcatgtt cccctccata
      301 ccccaaaacg agaaggcgtg tccatgtccc tgcccttcac ccaaggggct tctctgaggt
      361 gcacacggtc aggcccccgg ggccgtgccg tgcgcccccg ggtgtccccg gcgtccatgg
      421 gctcggcaga gggggtcccc ccccactggc ctttgcgatc ccctgtcatt tccgctgctg
      481 tccgagcagg tccctgatct cggtgagcag gaccacttcc ttggtgggtt ctgcgggctt
      541 ctcctcttcc ttgcgcttga acgcgttgat gcccttgacg agcaggaaga tggcgaaggc
      601 tatgatgagg aagtccacca cggtctgtat gaacttgccg tagttcaggg tcacagccgc
      661 cgcctggtcg gatgcggcct tgagggtgat ggcgaggtcg gtgaagtcca cgccgcctaa
      721 gagcaccccg atgggcggca tgatgacgtc cgccaccagc gatgacacga tcttgccgaa
      781 cgcggcgccg atgatgatgc ccaccgccat gtccaccacg ttgcccctca tcgcgaattc
      841 cttgaactcc ttgatcatgc ccatgccggt acctcctaaa agtttgtaaa aaggtgcggg
      901 attcccaagg tgagcggctc gaggaaagag tagcccagaa cccacggcga tgcaaggatc
      961 cagaaggggg atggggagga tcgcgcatgg tcttcttggg ccgcttcatc gcctgggccg
     1021 ggggcggggc cgcctttggc ggcactcgat gacctgcatg gggggcggga cgccgcagct
     1081 tgtgggttcg cccccgtctc gtgggcgtgc cagtcccggg cttgcgggtg cgacctcgcg
     1141 ccggcggata gggttcatgc cggcctcgat ccccgccggg acagggaaga tggcggtgcg
     1201 ggg
//
LOCUS       DAOSCR010000744         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_79583_length_1195_cov_3.251754, whole genome shotgun sequence.
ACCESSION   DAOSCR010000744 DAOSCR010000000
VERSION     DAOSCR010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79583_length_1195_cov_3.251754"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLT69_13265"
     CDS             <1..380
                     /locus_tag="PLT69_13265"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOJ15455.1"
                     /translation="SWVTLVVNIAFCLALMPLMKHSGIALASTLSVFAQLAVQHRYLA
                     KLGVRLPKGFVMDVFKMVLASIAMGALIVPFALLDLWSGGPRAEAAGVLAVSILLGAF
                     VYFLVAWMLGASGLRRFVGRKGS"
     gene            677..805
                     /locus_tag="PLT69_13270"
     CDS             677..805
                     /locus_tag="PLT69_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15456.1"
                     /translation="MYLTRREILEQLERMGIRTLAELKRACREFESYWFSQRYRAR"
     gene            866..>1195
                     /locus_tag="PLT69_13275"
     CDS             866..>1195
                     /locus_tag="PLT69_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15457.1"
                     /translation="MWPERLNEYIVDKTLELTPDFIRNKADVIPVLFQVSLLSDVDVR
                     IEKISDLFLNFLDRITPYDVAFVYMWEPQNVWFCRGLQGEIPDSIESGDIFTCMIREV
                     AKPLLIKD"
BASE COUNT          243 a          321 c          358 g          273 t
ORIGIN      
        1 tatcctgggt gacactggtt gtgaacatcg cgttctgcct cgcattgatg cccctcatga
       61 aacattccgg catagcgctt gcgagcaccc tctcggtgtt cgcccagctg gccgtgcagc
      121 accgttacct cgcgaagctg ggggtgcgcc tgccgaaggg cttcgtcatg gacgtcttca
      181 agatggtcct ggcctccatc gccatgggcg ccctcatcgt gccgttcgcg ctcctcgacc
      241 tgtggtccgg cggtcccagg gcggaggcgg cgggtgttct ggccgtctcc atcctgctcg
      301 gggcgttcgt gtacttcctg gtcgcatgga tgctcggcgc ctcggggttg cgtcggttcg
      361 tcggccgcaa ggggtcgtga aggctcgagc tgccggcccc aaggacacgg cttgaagggg
      421 ttaaagatac acgtcagccg caaaggtcct tgaaggctca ggaagatgaa aatgtccata
      481 tgccagtata gccgcgtatg ggtatgaaat atatttcttt tcgtgagtaa caaaagatct
      541 acggatgaat cgatcctcat gtctcttttc ctcagatatt caatggaata acagcacgtt
      601 ggaaacatgg ggatccgtgg catcatgttt gctacctcat catgtcatac agcggagtcg
      661 catgggggtc cgggacatgt acctcacgag aagggaaata ctcgaacagc ttgaacggat
      721 gggcatcagg acccttgccg agctgaagag ggcctgcagg gaattcgagt cttactggtt
      781 ttcccagcgt taccgcgcaa gatgaatccg ggacccggat gcttgtcttg ccttgcgttg
      841 aggtggggag agggcgggat cttgtatgtg gcctgaacga ctgaacgaat atatcgtaga
      901 caagacgctc gaactcaccc cggatttcat acggaacaag gcggacgtca tcccggtgct
      961 cttccaggtc tccctcctgt ccgacgtgga cgtgaggatc gagaagatct cggatttgtt
     1021 cttgaacttc ctggaccgca taacacccta cgacgtggcc ttcgtgtaca tgtgggagcc
     1081 ccagaacgtc tggttctgca gggggctcca gggagagatc cccgacagca tagagtcggg
     1141 ggacatattc acctgcatga tacgcgaggt ggcaaagcct ctcttgatca aagac
//
LOCUS       DAOSCR010000745         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_79647_length_1195_cov_1.800877, whole genome shotgun sequence.
ACCESSION   DAOSCR010000745 DAOSCR010000000
VERSION     DAOSCR010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79647_length_1195_cov_1.800877"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..174)
                     /locus_tag="PLT69_13280"
                     /pseudo
     CDS             complement(1..174)
                     /locus_tag="PLT69_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(165..1049)
                     /gene="era"
                     /locus_tag="PLT69_13285"
     CDS             complement(165..1049)
                     /gene="era"
                     /locus_tag="PLT69_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOJ15458.1"
                     /translation="MSTGTKSGYVSILGRPNVGKSTFLNRVLEEKISITTSKPQTTRN
                     RILGIYNDPDSQIVFLDTPGIHEPHKALNRYMVETALATVSDADVALVMADHLDTPDK
                     LSVVVEPVARAAKPAVLALNKVDLMDQSGARARLEQLAAVHPFVHACAVSSTTGTGID
                     GLLSVVKSLLPEGPRYFPEDMITDCPVRFLCAELIREKVFTVTREEIPYATAVEIERF
                     DEGADPVVISATIHVERPSQKAIVIGAKGSMLKEIGTQARKEIERLLGRRVFLELFVK
                     VTKDWTKDPKRMRDLGYR"
BASE COUNT          188 a          392 c          379 g          236 t
ORIGIN      
        1 gtcggtaaac agcgcctcgc gccctcccgg cagcttcacg cgccgcgtcg tcgggtcgag
       61 ggtggcgaac agcatgtcct cagccaggac gttcgcgccc gtgagcgcgt tgaagagcgt
      121 ggacttgccc gcgttgggcc tccccacgat gaccactacc ggcatcagcg gtaccccagg
      181 tccctcatcc tcttgggatc cttcgtccag tccttggtga ccttcacgaa gagttccagg
      241 aagactctcc ggccgagcag gcgctctatc tccttgcgcg cctgcgtgcc tatctccttg
      301 agcatggagc ccttcgcccc tatcacgatc gccttctgcg aaggccgctc cacgtggatc
      361 gtagccgata tgaccaccgg gtcagcgccc tcgtcgaagc gctcgatctc gaccgccgtg
      421 gcatagggta tctcctcacg ggtcacggtg aacaccttct cccgtatgag ctccgcgcag
      481 agaaacctga ccgggcagtc cgtgatcatg tcctcgggga agtacctggg cccctcggga
      541 agcagcgact tgacgacgga aagaaggccg tcgatgcccg tgcccgtcgt ggacgacacc
      601 gcgcacgcgt ggacgaaggg gtggacggct gcgagctgct cgagcctcgc ccgggcgccg
      661 ctttggtcca tgaggtccac cttgttgagg gcgaggaccg cgggctttgc ggccctggcc
      721 accggttcga cgacgaccga gagcttgtcc ggcgtgtcga ggtgatccgc catgacgagc
      781 gccacgtccg cgtccgagac ggtcgcaagc gcggtctcca ccatgtagcg gttcagcgcc
      841 ttgtgtggtt cgtggatgcc cggggtgtcg aggaatacga tctgcgagtc agggtcgttg
      901 tagatgccga ggatgcggtt cctggtggtc tgtggtttgc tcgtcgttat ggagatcttc
      961 tcctcgagca cgcggttgag gaacgtggac ttgccgacgt tcggcctgcc gaggatggag
     1021 acgtaccctg acttcgttcc cgtcgacatc tcatgcgagc tcgagcttca tctggcgggc
     1081 gagaaccgag tgcaccggca gcctgacgga gaccgacgtc cccttccctt gctcgcttac
     1141 gacctctatg gtcccgccgt gacgcttcac gatcccgtag ctcaccgaga gcccg
//
LOCUS       DAOSCR010000746         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_80382_length_1187_cov_2.428445, whole genome shotgun sequence.
ACCESSION   DAOSCR010000746 DAOSCR010000000
VERSION     DAOSCR010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80382_length_1187_cov_2.428445"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLT69_13290"
     CDS             complement(<1..314)
                     /locus_tag="PLT69_13290"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ15459.1"
                     /translation="MKTDIEIAQSCELKPIAEVASSLGLSEDEIVPYGRHIAKVPLSV
                     MGRLADVPPGRLVLVTAMSPTPMGEGKTTTTIGLGQALWRLGRRAVMAIREPSLGPCM
                     GV"
     gene            complement(340..>1187)
                     /locus_tag="PLT69_13295"
     CDS             complement(340..>1187)
                     /locus_tag="PLT69_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOJ15460.1"
                     /translation="GIIAAITEKLGAYTINIEVSDVVAREDIFLMDLMCDIGKSALPV
                     ENLKPALKDTMRVIGIATMFQTEDVFNKKKKIVLFDIHSSFMDAASMREVMVHASIDP
                     RSVFREDPARTDASFMHETVGMLGALPVEVFKTVLEHARITPGTRELVQALKAMGYRI
                     GLVSSAFDVFTDFLRERLGLDYAFGLELPVDEDSQTVIGDLPEGLMSVPDRAAIVGQI
                     VGRERIDPEDVTVISDREAHFDATPGIRVAFDTKVLLDLMNSHALSRQTLTGMLRSFG
                     IRDAR"
BASE COUNT          196 a          383 c          376 g          232 t
ORIGIN      
        1 ttgacgccca tgcacgggcc gagcgacggc tccctgatcg ccatgaccgc acgcctgccc
       61 agcctccaga gggcctgccc gagccctatg gtggtcgtcg tcttcccttc gcccatgggg
      121 gtcggggaca tggccgtgac gagcacgaga cgtcccggcg ggacatcggc gagccttccc
      181 atcacggaca gggggacctt ggcgatgtgc ctcccgtagg ggacgatctc gtcctccgaa
      241 agcccgaggc tggatgcgac ttcagcgatg ggcttcagct cgcatgactg tgcgatctcg
      301 atgtctgtct tcatgcgacg agccgcctga ggtccctctt caccgggcat cgcgtatgcc
      361 gaagctcctg agcatgccgg tgagcgtctg cctgctcaac gcgtggctgt tcatgaggtc
      421 gagcaggacc ttggtgtcga acgcgaccct gatgcccggg gtcgcgtcaa agtgcgcctc
      481 cctgtcggag atcacggtga cgtcctcggg gtcgatgcgc tccctcccca cgatctgacc
      541 gacgatggcg gccctgtccg gcacgctcat gaggccctcc ggcaggtcac cgatcaccgt
      601 ttgagagtcc tcgtccacgg gcagttcgag gccgaaggca tagtccaacc ccagcctttc
      661 cctgaggaag tccgtgaaga cgtcaaacgc gctggatacg aggccgatcc ggtaccccat
      721 ggccttgaga gcctgaacga gctcccttgt cccgggcgtg atccgcgcgt gctccagaac
      781 cgtcttgaag acctccaccg gcagggcgcc gagcatcccg acggtctcgt gcatgaagga
      841 cgcatccgtc cgtgcgggat cttccctgaa gaccgagcgg gggtcgatgg acgcgtgcac
      901 catgacctcc cgcatggatg cagcgtccat gaagctcgag tggatgtcga acagcacgat
      961 tttcttcttc ttgttgaaca cgtcctcggt ctggaacatg gtggcgatgc ctatgacccg
     1021 catggtgtcc ttgagggcgg gtttcaggtt ctccacgggc agcgcgctct tgccgatgtc
     1081 gcacatcagg tccatgagga agatgtcctc ccgggccacg acgtcggaga cctcgatgtt
     1141 gatcgtgtat gcgccgagct tctcggtgat ggcggcgatg atgccgg
//
LOCUS       DAOSCR010000747         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_80547_length_1185_cov_3.521239, whole genome shotgun sequence.
ACCESSION   DAOSCR010000747 DAOSCR010000000
VERSION     DAOSCR010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80547_length_1185_cov_3.521239"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLT69_13300"
     CDS             <1..585
                     /locus_tag="PLT69_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOJ15461.1"
                     /translation="AVAARKVESLARAEARVRVVAPWVCDEIASTPGVEVCCRPYVED
                     DLEGAYLVVAASNDPEVNQAVSKDAARRGVLCNVVDTPRLCSFIVPSVVERGPIKVAI
                     STGGLSPALSRRLRLVVGSAIGDEYVTLAKVLGAIRPIVTSQPGSPDDHRRVFEVLID
                     SELLDAVRAGDRGRAEHILSAALGRPIDLREIMG"
     gene            585..>1185
                     /gene="ccsA"
                     /locus_tag="PLT69_13305"
     CDS             585..>1185
                     /gene="ccsA"
                     /locus_tag="PLT69_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOJ15462.1"
                     /translation="MHGLTLTLYALAGLGFVVQLIAGNTRFLGVLRVLYLLAASTLLS
                     SFVAAWVSSGLPSAATPQQALSMLVLFSSLALVPLVFRERTAALAAFFLPAAAIVLAF
                     SAPFSGPPGPVALAPHRMWYVLHTVSVIAGEALLLVASVSSGAYLLHERMIRHGDIHL
                     KISRLPPLTVLDWITAVSLASGFCAISAGMITGGLSATAT"
BASE COUNT          156 a          414 c          395 g          220 t
ORIGIN      
        1 gcggtcgcgg ctcgaaaggt ggaatccctg gcgagggccg aggcacgcgt gagggtggtg
       61 gccccctggg tctgcgacga gatcgcatcc acccccggcg tggaggtgtg ctgccggccc
      121 tacgtcgagg acgacctcga gggtgcgtac ctcgtggtgg cggcctcgaa cgaccccgag
      181 gtgaaccagg cggtgagcaa ggacgcggca cgacgagggg tcttgtgcaa cgtggtcgac
      241 accccgaggc tgtgttcctt catcgtcccg tcggtggtgg agaggggtcc catcaaggtg
      301 gccatatcca cgggcgggct ctctcccgcc ctgtcaagga ggttgcgcct ggtcgtgggg
      361 tcagccatcg gagacgagta cgtgacgctc gccaaggtcc tcggtgccat ccgccccatc
      421 gtcacgtccc agcccgggtc gcctgacgac caccggaggg tcttcgaggt gctcatcgac
      481 tcggagctcc tcgatgccgt gcgcgcaggg gacaggggcc gcgcggagca catcctctcg
      541 gcggccctgg gcaggcccat agacctgcgg gagatcatgg ggtgatgcac ggactgaccc
      601 tgaccctcta cgcgctcgca ggcctgggtt tcgtggtcca gctcattgcc ggaaacacga
      661 ggttcttggg cgtcctgagg gtcctctacc tgctcgcggc ctccaccctc ctctcctcgt
      721 tcgtcgcggc ctgggtatcg tcgggccttc cgagcgcggc cacgccgcaa caggcgctct
      781 ccatgctcgt cctcttcagt tccctggccc tcgtcccgct cgtgttcaga gaacgcaccg
      841 cggccctggc ggccttcttc ctccccgccg cggcgatcgt cctggcgttc tcggccccgt
      901 tttccggccc tcccggaccg gtggccctcg cccctcacag gatgtggtac gtgctccaca
      961 ccgtgagcgt gatcgcgggc gaggccctgc tcttggtggc ctccgtctca tcgggcgcct
     1021 acctcctcca cgagcgcatg atcaggcacg gggacatcca cctgaagatc tccaggctgc
     1081 cgcctttgac agtgctggac tggatcacgg ccgtttccct ggcatcgggg ttctgcgcga
     1141 tctcggcggg catgataacg gggggtctct cggcgacggc caccc
//
LOCUS       DAOSCR010000748         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_80553_length_1185_cov_3.114159, whole genome shotgun sequence.
ACCESSION   DAOSCR010000748 DAOSCR010000000
VERSION     DAOSCR010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80553_length_1185_cov_3.114159"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLT69_13310"
     CDS             <1..352
                     /locus_tag="PLT69_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15463.1"
                     /translation="VIIRNADLTYTDMWGRKTGEATMPLPDIAGIVAGDLPEGPYLLR
                     TTADGHARVFYAWGWLGVDEARMPRELHAWGMSGLDVTVDTSTADVRLEVKYGDDTLT
                     ILLEEIEGGRWATQ"
     gene            386..718
                     /locus_tag="PLT69_13315"
     CDS             386..718
                     /locus_tag="PLT69_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15464.1"
                     /translation="MMTVTCPTCGKKIVWDDFQPLEIKCSKCGERLNLHREFRRNIEI
                     REHGLPGRRFSCPRCRTVISRRWFVKCPGCNYWVFGPFVFYDKLAAALLIGIAYVVFS
                     AIYLIYFH"
     gene            756..1163
                     /locus_tag="PLT69_13320"
     CDS             756..1163
                     /locus_tag="PLT69_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOJ15465.1"
                     /translation="MSDTFISPEKVKALAQRMKALAISERDLVEIFVRARGRGGQKVN
                     KTSSCVHLKHVPTGIEVKCDKSRSREANRFFARRILADKIEDLASEGSSRRAVEIARA
                     RKRKATRARKARKKLELKRGSQTEASDAGGPGD"
BASE COUNT          260 a          332 c          407 g          186 t
ORIGIN      
        1 ggtcatcata aggaatgccg acctcacgta cacggacatg tgggggagga agacagggga
       61 ggccacgatg cccctgccgg acatcgctgg gatcgtcgcg ggtgacctgc ccgaagggcc
      121 gtacctcctc aggacgaccg cggacgggca cgcacgggtc ttctacgcat ggggatggct
      181 gggcgtggac gaggcacgca tgccccgtga actccatgcc tgggggatga gcgggctcga
      241 cgtgacggtc gacacatcga cggctgacgt gagactcgag gtgaagtacg gcgacgacac
      301 actcaccatt ctcctcgaag agatcgaggg cggcaggtgg gcgacgcaat gagaacgcgc
      361 aagggaaagg ggtgaacaag gccatatgat gactgtcaca tgcccgacgt gcgggaagaa
      421 gatcgtgtgg gacgatttcc aacccttgga aatcaagtgc tccaagtgcg gcgagcgcct
      481 gaaccttcac cgggagttca ggcggaacat cgagatcagg gaacacggcc tcccgggaag
      541 gcgtttctcc tgcccgaggt gcaggacggt catatccagg aggtggttcg tcaagtgccc
      601 ggggtgcaac tactgggtct tcggcccgtt cgtcttctac gacaagctgg cggccgcgct
      661 cctcatcggg atcgcctacg tggtcttctc ggcgatctac ctcatctatt tccactgaga
      721 agccccaagg gatgggcgtt tgagtcacgc ggcccatgtc cgacacgttc atcagcccgg
      781 aaaaggtcaa ggccctcgca caacgcatga aggcgctcgc gatcagcgag cgggatctcg
      841 tggagatctt cgtgagggcc aggggaaggg gaggccagaa ggtgaacaag acctcctcct
      901 gtgtacacct caagcacgta cccacaggga tcgaggtcaa gtgcgacaag tcgcgctcga
      961 gggaggccaa caggttcttc gcgcgaagga tcctcgcgga caagatcgag gatctcgcct
     1021 ccgagggatc ttccaggcgg gccgttgaga tcgccagggc caggaagcgg aaggcgacaa
     1081 gggcgaggaa ggcgagaaaa aagctcgagc tgaagagagg ctcgcagacg gaagcctccg
     1141 acgccggcgg gcccggggac tgagtgtccg cgcgtacttg ggatc
//
LOCUS       DAOSCR010000749         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_81014_length_1181_cov_3.075488, whole genome shotgun sequence.
ACCESSION   DAOSCR010000749 DAOSCR010000000
VERSION     DAOSCR010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81014_length_1181_cov_3.075488"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..667
                     /locus_tag="PLT69_13325"
     CDS             332..667
                     /locus_tag="PLT69_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOJ15466.1"
                     /translation="MGILLRWIIMTASVMIAAYLLPGVTVQGFWAALITALVLGLVNA
                     LLRPVLILLTLPLTVVTLGLFIFVVNGLLVLMAAWIVDGFHVAGLGWAILFSIVVSIV
                     SFVFHQVFG"
     gene            complement(863..>1181)
                     /locus_tag="PLT69_13330"
     CDS             complement(863..>1181)
                     /locus_tag="PLT69_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15467.1"
                     /translation="ITGGVNIFPTEIESAIKRHDKVMDVAVVRYPHPDLGEVPAAVIQ
                     PKDGQSIDASEIVEHLKSLAFTSFKIPRHIEFVKTLPRHIDGKMMKRQIEDRFWQGIE
                     RRG"
BASE COUNT          151 a          381 c          415 g          234 t
ORIGIN      
        1 gggtggggcg gggtttgccg ggcggatggc tgggcccgtt ctcctggagg atccatgcgc
       61 aaggacgcca aagaaacgcg gccttgcgcg tgggaggaga ggccggggtt ctgcctgcgg
      121 gcgggcctcc accgggtggg tgctcatcca gccctgcctg atgcccgttc cgactgctgc
      181 atgtgcagcc gggaagggcc gcgttcggac gtgagacgcg gcccggccgc gccttgggcc
      241 tcgagggcgg acctcatcga gggcatcacg gcctctggca cgcttgaaac atcccgatcg
      301 cgggtgtacg atgcaggcag ggggaatcgc catgggtatc ctgctcaggt ggatcatcat
      361 gacggcgtcg gtcatgatcg cggcctacct cctgcccggt gtcacggtgc agggcttctg
      421 ggccgccctc atcactgcgc tcgttctggg gctcgtcaac gccctgctca ggcccgtgct
      481 catcctgctg accctgcccc tcaccgtggt gacgctcggc ctcttcatct tcgtcgtcaa
      541 cgggctgctc gtgctgatgg ccgcctggat cgtcgacggc ttccacgtgg ccgggctggg
      601 gtgggcgatc ctgttcagca tcgtcgtgtc catcgtgagc ttcgtgttcc accaggtgtt
      661 cggctgagga aggctcggtc caggccctca ccggatccct gcggtggctc gaggggcgtt
      721 ttcggcctgc acggcggcgc agcgaccgcg acaccctgac cggtggcgta aggcacttcc
      781 gagggagggc catgcccctc tccagcctgt catgggcgag gggctgtcgg tctgccccgg
      841 tggccccacg ccgggcgccg cctcaccccc tgcgttcgat gccctgccag aagcggtcct
      901 cgatctgcct cttcatcatc ttgccgtcta tgtggcgcgg cagggtcttc acgaactcga
      961 tgtggcgggg gatcttgaag ctcgtgaagg ccagggattt cagatgctcg acgatctcgg
     1021 aggcgtcgat cgactggccg tccttgggct ggatgaccgc ggcgggcacc tcgccgaggt
     1081 cggggtgggg gtaccggacc acggcaacgt ccatgacctt gtcgtggcgc ttgatcgccg
     1141 actcgatctc ggtggggaat atgttcaccc cgccggttat g
//
LOCUS       DAOSCR010000750         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_81114_length_1180_cov_2.846222, whole genome shotgun sequence.
ACCESSION   DAOSCR010000750 DAOSCR010000000
VERSION     DAOSCR010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81114_length_1180_cov_2.846222"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..>1180)
                     /gene="murD"
                     /locus_tag="PLT69_13335"
     CDS             complement(219..>1180)
                     /gene="murD"
                     /locus_tag="PLT69_13335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOJ15468.1"
                     /translation="EIEVASSMLRGTIVGVTGTNGKTTTTTLVHMILEAMGYDAGIGG
                     NISPPLISLVDRDPDYLAVEISSFQLEWIENFRPKIAVCLNITPDHLDRYRSMEEYVH
                     YKLRIFENQTADDIAVISGDDPYLKGLHLRPKTAVFSTLGAPEGDGAYVDGGRIVFTG
                     SIAGGGPRVPPAEAVGSGVIEDMLAASIAGRYLGVDVDVMERVFMGFKVIHHRYERVD
                     TIGGVTFVDDSKATNVGALDKALSTLGTPVTLILGGKDKGGDFSDIALRYRPLIRMAV
                     VMGEAADRISRRWAESCRASGRETWRRRSGWHTRHPGRATRCS"
BASE COUNT          197 a          399 c          361 g          223 t
ORIGIN      
        1 gacccagccc gctgcccagg cgttccttgg agatgaagaa gctcgacgac aggatcatca
       61 ccgtgcccat catgatcagg accacggtga ccaggaagaa gatcacgtcg aagtctatct
      121 cgaggacgtg tgcagcctga tccctcggac gcattccctg aacacctcac ccctgtgtgc
      181 gtagctgttg aacatgtcga agctcgcgca gcccgggctc aagagcaccg tgtagcccgg
      241 cctggatgcc tcgtatgcca gcctgaccgc ctcctccatg tctcgcgccc tgacgcacgg
      301 cacgattccg cccatctccg cgatatgcga tccgccgcct cgcccatgac caccgccatc
      361 cgtatgagcg ggcggtacct gagcgcgatg tccgagaagt cacccccctt gtccttgcct
      421 cccaggatga gcgtcacggg ggtgccgagc gtggacaggg ccttgtccag ggcccccacg
      481 ttggtcgcct tggagtcgtc cacgaaggtg acgccgccta tggtgtccac gcgctcgtag
      541 cggtgatgga tcaccttgaa acccatgaac acccgttcca tgacgtcgac gtccaccccg
      601 aggtacctgc ccgcgatcga ggcggcgagc atgtcttcga tgacccccga gcccacggcc
      661 tcggccggcg ggacacgcgg accgcccccg gcgatcgagc cggtgaacac gatcctcccc
      721 ccgtccacgt acgcgccgtc gccttctggg gcgccgagcg tcgagaagac ggcggtcttc
      781 ggcctcaggt ggagcccctt caggtatggg tcgtcgccgc tgatgacggc gatgtcgtcc
      841 gcagtctggt tctcgaagat cctgagcttg tagtgtacgt actcttccat gctccggtaa
      901 cggtcgaggt ggtcgggcgt gatgttcagg cacaccgcga tcttgggcct gaagttctcg
      961 atccattcca gctggaacga gctgatctcg acggccaggt agtccgggtc cctgtcgacc
     1021 agggatatga gcggtggcga gatgttgccg ccgatgcccg cgtcgtaccc catggcctcg
     1081 aggatcatgt ggacgagggt ggtggtcgtg gtcttcccgt tcgtccccgt gacgcccacg
     1141 atggtgcccc tgagcatgga cgatgcgacc tcgatctccg
//
LOCUS       DAOSCR010000751         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_81536_length_1176_cov_4.234612, whole genome shotgun sequence.
ACCESSION   DAOSCR010000751 DAOSCR010000000
VERSION     DAOSCR010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81536_length_1176_cov_4.234612"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLT69_13340"
     CDS             <1..496
                     /locus_tag="PLT69_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ15469.1"
                     /translation="LILTHGHVDHIGSVSFFRERYGCTVVAHDLDADPIERGDPGPTA
                     AGWYGVKLPRVKVDRRLYAGSEVVLVGETELHCLHTPGHTPGSVSVYLDRDGTRILFG
                     QDIHGPFSTEFGSDMTAWRASMEALLALGADILCEGHFGIYAPRERVERYIRGYLDRY
                     ARRW"
     gene            763..>1176
                     /locus_tag="PLT69_13345"
     CDS             763..>1176
                     /locus_tag="PLT69_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ15470.1"
                     /translation="MARSYNIAVIPGDGTGPEVVAEGIKVLEAVGAAEGISFDFTTYD
                     FGGERYLKTGETLPDSALEELRKFDAIYLGAIGHPDVKPGILEKGILLRIRFELDQYI
                     NLRPVKLYPNVETPLRDKGPEHIDYVVVRENSGGVY"
BASE COUNT          219 a          364 c          410 g          183 t
ORIGIN      
        1 gctcatcctc acgcacggcc acgtggacca catcgggtcg gtctcgttct tccgcgagag
       61 atacgggtgc acggtcgtgg cccacgacct cgacgccgac cccatcgagc ggggggaccc
      121 ggggcccacc gcggccgggt ggtacggtgt caagctgccg agggtcaagg tggacaggag
      181 gctgtacgcc gggtccgagg tggtcctcgt gggcgaaacc gaactccatt gcctgcacac
      241 ccccgggcat acgcccggct ccgtctcggt ctacctggac cgcgacggca cgcgcatcct
      301 gttcggacag gacatccacg gcccgttcag caccgagttc ggctcggaca tgaccgcctg
      361 gagggcctcc atggaagcgc tgctcgccct cggcgcggac attctgtgcg agggccattt
      421 cggcatctac gcgccaaggg agcgcgtgga gagatacatc aggggctacc tcgaccggta
      481 tgcaaggcgg tggtgaggcc gcctgcgatg aaccatgggc ggggtcgccc caaggccggg
      541 acggacggcg gcctctcccc gggcatgtgc gcgtggggat gaaaaaccgg gatcgcccgg
      601 agacacggtg cagcccccgg gcagcatcca ggttcacgcc tgccgcacga ggcgcatcag
      661 ggcttgactg caggcgagct ttcagtgtat cgcgcatacc cattgcgtcc gtctgcgagc
      721 agggtgcaga atgaagaaag tcaagatcca gggggatatg ccatggcgcg gtcgtacaac
      781 atagccgtca tcccggggga cgggacgggg cccgaggtcg tggccgaggg gatcaaggtc
      841 ctcgaggcgg tcggcgctgc ggaagggatc tctttcgatt tcacaaccta cgacttcggg
      901 ggtgaacgat acctcaagac cggcgagacg ctgcccgact cggcgctgga ggagctcagg
      961 aagttcgacg ccatctatct gggcgccata ggccacccgg acgtgaagcc ggggatcctc
     1021 gagaagggga tcctcctgag gatccgcttc gagctggacc agtacatcaa cctcaggccg
     1081 gtgaaactct acccgaacgt ggagacgccg cttagggaca agggtcccga gcacatcgac
     1141 tacgtggtgg tgagggagaa ctcggggggc gtctat
//
LOCUS       DAOSCR010000752         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_81670_length_1175_cov_2.347321, whole genome shotgun sequence.
ACCESSION   DAOSCR010000752 DAOSCR010000000
VERSION     DAOSCR010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81670_length_1175_cov_2.347321"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1175
                     /locus_tag="PLT69_13350"
     CDS             <1..>1175
                     /locus_tag="PLT69_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOJ15471.1"
                     /translation="VTLMNTSRACGLDLGLLSECSELLEEVSRTYRHLLDDRTSIVDA
                     NVLLHQIPGGMISNLVNQLREMDALPRLKDVYGELPRVRKDLGQVPLVTPTSQIVGIQ
                     AVNNVLFDKPGERYSMITAQVKDLCYGLYGKTPAPIDEQVRTKALKDHPRGSTPIHVR
                     PADILEPELEKAREETAGLARDIDDVLVYALYPTTGKRFLRWKYGLEEIPAEVTGEGT
                     SMPSRQGPPPAQGKDAAEGKCTGGDSLRRDVQEGLGGPRRDENAGFPVGATRYRLYVD
                     GQCVEFALEQVAHPAGGLVSRGGSQRPGLELREKRGLPADPVHVRHEVRNEEGVAREG
                     TGGEAPGSPGDGDTPVTAPMPGMVVRYVLGEGERVAKGDVLVVLEAMKMEIFNTAPS"
BASE COUNT          242 a          357 c          414 g          162 t
ORIGIN      
        1 cgtgacgctc atgaacacga gcagggcctg cgggctggat ctcgggctgt tgagcgagtg
       61 cagcgagctc ctcgaggagg tgtcgcgaac ataccgtcac ctcctcgatg accgcacctc
      121 catcgtggac gcgaacgtcc ttttgcacca gatacccggg ggcatgatct ccaacctcgt
      181 gaaccagctc agggagatgg acgccctgcc caggctcaag gatgtctacg gcgaactccc
      241 gagggtccgc aaagacctgg gccaggtccc cctcgtcacg cccacgagcc agatcgtagg
      301 tatccaggcg gtcaacaacg tcctcttcga caagccgggc gaacgctact ccatgatcac
      361 cgcccaggtg aaggacctct gttacggcct ctacgggaag acacccgcgc cgatcgacga
      421 acaggtgagg acgaaggcgc tcaaggacca cccgagggga tcgaccccca ttcatgtcag
      481 gccggcggac atactggagc cggagctcga aaaggcgcgc gaggagaccg ccggccttgc
      541 ccgcgacatc gacgacgtgc tcgtctacgc cctgtacccg accaccggca agaggttcct
      601 caggtggaag tacgggctcg aggagatccc cgccgaggtg acaggcgagg ggacatccat
      661 gcccagccgg caaggaccgc ccccggctca gggaaaggac gccgcggagg gaaagtgcac
      721 aggaggagac agcctgagaa gggatgtcca ggaggggttg ggcggaccga gacgggatga
      781 aaatgccggc ttcccggtcg gggcgacgag gtacaggctg tacgtggacg ggcaatgcgt
      841 cgagttcgcg ctcgagcagg tcgcacatcc tgcaggcggt ctcgtatcca gggggggctc
      901 gcagaggccg ggtctcgagc tgagggagaa gagggggctt cccgcagacc cggtgcacgt
      961 cagacacgag gtgcgaaacg aggaaggcgt cgcaagggag ggcacgggcg gtgaggctcc
     1021 aggctccccc ggcgacggtg acaccccggt gacggcgccc atgcccggga tggtggtcag
     1081 gtacgtcctc ggcgagggtg agcgcgttgc gaaaggggac gtgctcgtgg tcctcgaggc
     1141 catgaagatg gagatcttca atacggcgcc cagtg
//
LOCUS       DAOSCR010000753         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_81752_length_1174_cov_2.806971, whole genome shotgun sequence.
ACCESSION   DAOSCR010000753 DAOSCR010000000
VERSION     DAOSCR010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81752_length_1174_cov_2.806971"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLT69_13355"
     CDS             complement(<1..186)
                     /locus_tag="PLT69_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15472.1"
                     /translation="MRRLILTFTFAAIASLVMSACSTSSTTGLLRGKDSSHALIELLR
                     QAESGDSQAQFNVGLIHC"
     gene            1010..>1174
                     /locus_tag="PLT69_13360"
     CDS             1010..>1174
                     /locus_tag="PLT69_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOJ15473.1"
                     /translation="MKKILSGNEAVARGAFEAGVSFAAAYPGTPSTEILETISELYKD
                     IHAEWAPNEKV"
BASE COUNT          222 a          357 c          384 g          211 t
ORIGIN      
        1 gcagtggatc aggcccacgt tgaactgcgc ctgggaatca ccgctttcgg cctgtctcaa
       61 gagctcgatg agcgcatgcg aggagtcctt gccgcgcaag agacccgtgg tggacgaggt
      121 gctgcaggcg ctcatgacga gcgaggcgat ggccgcaaac gtgaacgtca ggatcaagcg
      181 tctcatgggt atccctcccg tcaagccatt gaactccatg ttctcctcga ttcatccgta
      241 taagcaagaa tcggaaatta catagtacca cataaacatc cattgttcaa gaacccaagt
      301 gttcgttatg cacggtaaac gtaataagcg agtcgtctct cgggtgggtt cgctctgacc
      361 gggcacctcg tctccttccc ggcggcgccg tggcgctggt accccgggcc cgcaggtctt
      421 ggggcctgcg accgagggcc ttaaggcatg aaaaggaaga gggttgcacc gatgaacgtc
      481 ctcctcacgg ctgggccatg cctcgcgggc ctgcccccct ggcgacgcgc acggcacctc
      541 acgtgcacca ggcgcccggc ggccacatcc tgcacccgtc tcccccgggg cgactccggg
      601 gggatgtctc cggctccgtg gtcgcatcgc gggcacacgc ggtttggacc cagggtcgca
      661 ggcatcgccg ttacgtgcga ggcgtcgtcc ctcgacggcg cgtctctggc cccatgtggg
      721 tgggtgtgcg cgaggggggc ttcttgcacc gtggtggtac ccgcgtgcct cggaggtgag
      781 cgccagaaat gccttgacag gatgtccgca ttcacgggat attaggcggc gatggagcga
      841 gaaacgccca ggacctgcgg gtacggctat tattacagca gactgtatcg ctgcgcctgc
      901 ccgtagtccc gggtgcgtgc gggaaaggac acggggccat gggcagaaac ccatggccct
      961 ttttccttag gagtataact acgttcgaac aggggcgagg atacacgtga tgaagaagat
     1021 cctcagcggc aacgaggcgg tggcgcgcgg ggcgttcgag gcgggggtgt ccttcgcggc
     1081 ggcgtacccc ggcacaccca gtaccgagat cctcgagacc atctcggagc tctacaagga
     1141 catacacgcc gagtgggccc cgaacgagaa ggtc
//
LOCUS       DAOSCR010000754         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_81938_length_1172_cov_3.123545, whole genome shotgun sequence.
ACCESSION   DAOSCR010000754 DAOSCR010000000
VERSION     DAOSCR010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81938_length_1172_cov_3.123545"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="argS"
                     /locus_tag="PLT69_13365"
     CDS             complement(<1..838)
                     /gene="argS"
                     /locus_tag="PLT69_13365"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ15474.1"
                     /translation="MKEAIEHLLREAIGDLVRSGAIPPVDTDRVSLEVPPEKTFGDFS
                     TNIALVSAKKAKMPPQKLASTIASTLARHDDVFERVEVAGPGFINLHVAPGVWIKELR
                     RIVELKASYGRSNAGRGRKVQVEFVSANPTGPLHVGHGRGAAVGDTLARILDAAGFDV
                     QTEYYVNDVGNQMNNLGASVLHRYRELHGVESAFPENGYKGDYIREIAAELKAERADS
                     LMGMPEDEALRVCRDFAAASIMNGIRKDLELFNVRFDRWFNESSLYEAGKVALFAIDE
                     AHC"
BASE COUNT          187 a          392 c          360 g          233 t
ORIGIN      
        1 cgcagtgcgc ctcgtcgatg gcgaacagcg cgaccttgcc ggcctcgtac aggctcgact
       61 cgttgaacca cctgtcgaac ctgacgttga acagctcgag gtccttcctg atcccgttca
      121 tgatggacgc ggcggcgaaa tcgcgacaga ccctcagggc ctcatcttcc ggcatgccca
      181 tcaggctgtc ggcgcgctcc gccttcagct ctgccgcgat ctccctgatg tagtcgccct
      241 tgtagccgtt ttccggaaag gcgctctcga cgccgtggag ctcccggtag cgatggagca
      301 ccgatgcgcc caggttgttc atctggttgc ccacgtcgtt gacgtagtac tcggtctgca
      361 cgtcgaaacc cgccgcgtcc agtatgcggg cgagggtgtc ccccacggcg gcgcccctgc
      421 cgtgccccac gtgcaggggg cccgtggggt tggcgctcac gaactccacc tgcaccttcc
      481 tgcccctgcc cgcattcgac ctgccgtagg aggccttgag ctccacgatg cgcctgagct
      541 ccttgatcca gacgccgggc gcgacgtgga ggttgatgaa gccggggccg gccacctcca
      601 ccctctcgaa cacgtcgtcg tgccgggcga gcgtcgatgc gatggtggag gcgagcttct
      661 gggggggcat cttggccttc ttcgcggaca cgagcgcgat gttcgtcgag aagtcgccga
      721 aggtcttctc cggcggcacc tcgaggctca ccctgtcggt gtccacgggt gggatggctc
      781 cggaccggac gaggtctcct atcgcctccc tcagcaggtg ttcgatcgct tccttcatcg
      841 gatcctcggt gtcgtaacct ttcgaggggg cgcaccccct ctgtcccggt gcctctttat
      901 tactaaaacc acctccttgt ccagtcgtga atgagagggt ggacgcatgc atcctgcgtg
      961 aaggccataa cgcggcgggg cctcaccccc gccgccgtcc gtaccggtcc gcccgtcccc
     1021 acccgggtgc tcggatattg acatgggggg cccgagcttc tataaggtga ttgttagcag
     1081 acagagtcat cgagtgctaa ggtccgccgc catgaccgag ttgagcgaca ggaacaagaa
     1141 gatactgaac gccatcatac gggaatacgt cg
//
LOCUS       DAOSCR010000755         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_82270_length_1169_cov_2.807899, whole genome shotgun sequence.
ACCESSION   DAOSCR010000755 DAOSCR010000000
VERSION     DAOSCR010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82270_length_1169_cov_2.807899"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLT69_13370"
     CDS             complement(<1..332)
                     /locus_tag="PLT69_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ15475.1"
                     /translation="MIWETVLLAIRTIRRNVLRSSLTILGSVIGVASVITMVTLGKGA
                     TLQVASEIASLGSNMLQARPGQGFRPPGGARSEAPPFKLTDADAIREQVGGLEAVAPL
                     ATKGARAL"
     gene            complement(329..1018)
                     /locus_tag="PLT69_13375"
     CDS             complement(329..1018)
                     /locus_tag="PLT69_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ15476.1"
                     /translation="MELMNITKVYGAGEAAVHALRGVDLRVDKGEFVAIMGPSGSGKS
                     TCMNILGCLDTPTSGSYLFRGVDVGGLGRDGRALLRRNHLGFVFQGFNLLSRTSALEN
                     VELPLVYRGVPARLRHKRAREALELVGLAGWEGHTPSELSGGQQQRVAIARAIVIEPS
                     VILADEPTGNLDSVSGREIMDLLCRFNRERGITVIMVTHDEHMAAYARRTIRFFDGLI
                     ASEDHNGGACT"
BASE COUNT          171 a          418 c          389 g          191 t
ORIGIN      
        1 tacaaggccc gtgcgccctt ggtcgcgaga ggggccacgg cctcgagccc gcccacctgc
       61 tcccttatgg cgtcggcgtc tgtgagcttg aacggcgggg cctccgagcg cgcgccgccg
      121 ggcggcctga acccctggcc cggccttgcc tggagcatgt tgctgcctag ggacgcgatc
      181 tcggaggcga cctgcagggt cgctcccttg cccagggtga ccatggtgat gaccgaggcc
      241 acgccgatca cgctccccag gatggtgagg ctcgagcgca acacgttgcg ccggatcgtg
      301 cggatcgcga gcagcaccgt ctcccagatc acgtgcacgc ccccccgttg tgatcctccg
      361 aggcgatgag gccgtcgaag aaccggatcg tgcgccgggc ataggccgcc atgtgctcgt
      421 cgtgcgtcac catgatcacg gttatcccgc gctccctgtt gaacctgcag aggaggtcca
      481 tgatctccct tcccgagacg gagtcgaggt tcccggtggg ctcgtccgcg aggatcacgg
      541 aaggctcgat cacgatggcg cgtgctatgg cgaccctctg ctgctggcct cccgagagct
      601 cggagggcgt gtgaccctcc cacccggcga gcccgacgag ctccagcgcc tcgcgcgcac
      661 gcttgtgcct gaggcgggcc ggcacgcccc tgtagacgag ggggagctcg acgttctcca
      721 gggccgaggt gcggctcagg aggttgaacc cctggaacac gaagcccagg tggttcctgc
      781 ggagcagggc gcgaccgtcg cggccgaggc cccccacgtc cacgcccctg aacaggtagg
      841 acccggaggt gggcgtgtcg aggcagccga ggatgttcat gcacgtggac ttgcccgagc
      901 ccgacggccc catgatggcc acgaactcgc ctttgtccac gcgcaggtcc acgcccctga
      961 gcgcgtgcac cgcggcctcg cccgcgccgt agaccttggt gatgttcatg agctccatga
     1021 gggagtgcgg gccgtttccg ctcacagccg tcctcacccc ttcttcgccg cgtcgaccac
     1081 caggggcgtg cccggggtca gatcgccgct cttcacctcg gtcatgctcc cgtccgtcga
     1141 gcccgtcacg atctcgatcg gcacgagac
//
LOCUS       DAOSCR010000756         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_82413_length_1168_cov_2.130279, whole genome shotgun sequence.
ACCESSION   DAOSCR010000756 DAOSCR010000000
VERSION     DAOSCR010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82413_length_1168_cov_2.130279"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLT69_13380"
     CDS             complement(<1..754)
                     /locus_tag="PLT69_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ15477.1"
                     /translation="MISVEGLWKSYGSQVLLEGVSFKVNQKERVGLVGRNGHGKTTLM
                     RIIAGLEEPDTGTVIIPRNYRIGYVTQHLDFTEATVLAEGMKGLRGDGRDQAWKVEKV
                     LMGLGFSAEDMHRHPHEFSGGFQVRLNLAKVIVSEPDMLLLDEPTNYLDITSIRWIER
                     FLSSWPRELILITHDRTFMDRIVTHTMGIHRRKVRKVEGDTSKYYTQIAQDEEVYERT
                     RVNEERKRKEIEEFIAQFRAKARLVGLVQSRIR"
     gene            complement(751..>1168)
                     /locus_tag="PLT69_13385"
     CDS             complement(751..>1168)
                     /locus_tag="PLT69_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15478.1"
                     /translation="LHRCRGAAETLVVEGFFDTLLVDQVRLIPVVGIGRGGDAVRMLD
                     KAVLFGCRSFTLVPGASREGLARAAETARAMAEGGLDVHVMELPKGYGDLDEFIRKTC
                     LDRFGALLKGRKRAGEWLERVGPHVSGTTPRQRGGH"
BASE COUNT          183 a          409 c          342 g          234 t
ORIGIN      
        1 gccttatgcg cgactgcacg agtccaacga gcctcgcctt ggccctgaac tgcgcgatga
       61 actcctcgat ctccttgcgc ttgcgctcct cgttgacccg cgttcgctcg tagacctcct
      121 cgtcctgtgc aatctgggtg tagtacttgg aggtgtcccc ttccaccttg cgcaccttgc
      181 gcctgtggat gcccatggtg tgggtcacga tcctgtccat gaaggtgcgg tcatgggtga
      241 tgaggatgag ttccctcggc cacgacgaga gaaaccgctc gatccacctg atggacgtga
      301 tgtccaggta gttcgtgggc tcgtcgagga gcagcatgtc cggctcggag acgatcacct
      361 tcgccaggtt gagcctcacc tggaagccgc ccgagaactc gtggggatgc ctgtgcatgt
      421 cctcggccga aaacccgagg cccatgagca ccttttccac cttccacgcc tggtccctgc
      481 cgtcgcctcg aaggcctttc atgccctccg cgagcacggt cgcctcggtg aagtccaggt
      541 gctgcgtgac gtagccgatg cggtagttcc tcgggataat gacggtgccc gtatcgggct
      601 cctcgagccc ggctatgatg cgcatgaggg tcgtcttgcc gtggccgttg cggccgacga
      661 ggcccacgcg ctccttctgg ttgaccttga aggagacgcc ctcgagcaag acctgggacc
      721 cgtaactctt ccagagaccc tcgacgctga tcaatgcccg cccctctgac gtggtgtcgt
      781 gcccgagacg tgcggcccca ccctctccaa ccactcgccg gccctctttc tgcccttcag
      841 gagggcgccg aacctgtcga gacacgtctt ccttatgaat tcgtcgagat ccccgtatcc
      901 cttcgggagc tccatgacgt ggacgtcgag gccgccttcg gccatggccc ttgcggtctc
      961 ggccgcccgg gcgagcccct cccgggatgc accgggcacc agggtgaagc tcctgcagcc
     1021 gaagaggacc gccttgtcca gcatgcgcac ggcatcccct cccctgccga tccccacgac
     1081 cgggatcagc ctgacctggt cgacgagcag ggtgtcgaag aatccctcga ccaccaacgt
     1141 ctccgcagct ccccggcatc ggtgcagc
//
LOCUS       DAOSCR010000757         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_83090_length_1161_cov_2.690778, whole genome shotgun sequence.
ACCESSION   DAOSCR010000757 DAOSCR010000000
VERSION     DAOSCR010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83090_length_1161_cov_2.690778"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLT69_13390"
     CDS             complement(<1..947)
                     /locus_tag="PLT69_13390"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOJ15479.1"
                     /translation="MGMTIAEKIIAAHAGMETVRTGEIVRVTVDVALANDITAPIAIQ
                     EFRKADRPALAHPDRVVFVLDHFTPTKDIASANQCKFVRDFALEHGMKHFYEGGEAGV
                     EHALLPEKGLVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGQAWMKVPPS
                     LKFEFTGELRPHVGGKDLILHVIGLIGVDGSLYKVMEFTGDTISRLCMADRMSMCNMA
                     IEAGGKAGIIAPDAVTEEYVSKRASRPYRVFASDDDAAYERVVSVDSASIEPLVAFPH
                     LPENVRPAREAGHVRIDQVVIGSCTNGRIEDLRVAAGFL"
     gene            complement(958..>1161)
                     /locus_tag="PLT69_13395"
     CDS             complement(958..>1161)
                     /locus_tag="PLT69_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOJ15480.1"
                     /translation="KSFSIKAITGGTDAQGEVTIELEDKGKTAVGQGSDPDILVACAK
                     ALVNAMNRMEFLKGRKAMKTPSL"
BASE COUNT          171 a          399 c          368 g          223 t
ORIGIN      
        1 ttgaggaagc ctgccgccac gcgcaggtcc tcgatgcgcc cgttggtgca ggaaccgatc
       61 accacctggt cgatcctcac gtggccggcc tcccgtgccg ggcggacgtt ctcgggcagg
      121 tgggggaacg ccacgagggg ctctatggag gctgagtcga cggagacgac ccgttcgtat
      181 gccgcgtcgt cgtcgctcgc gaacacccgg taaggccgtg acgccctctt tgacacgtat
      241 tcctcggtga ctgcgtccgg cgcgatgatc cctgccttgc cgcccgcctc gatggccatg
      301 ttgcacatgc tcatgcggtc ggccatgcac aggcgcgata tggtatcgcc cgtaaactcc
      361 atgaccttgt acagcgagcc gtcgacccct atcaagccta tcacgtggag gatcaggtcc
      421 ttccctccca cgtggggcct gagctccccg gtgaactcga acttcaggct cggcggaacc
      481 ttcatccacg cctgcccggt gagcatggcg gcagcgaggt cggtgctgcc cacaccggtg
      541 gcgaacgcgc cgagcgcgcc gtaggtgcac gtgtggctgt ccgcgccgat gaccaggtcg
      601 ccgggcagca cgagcccctt ctcggggagc agcgcgtgct ccacacccgc ctctccgccc
      661 tcgtagaagt gtttcatgcc gtgctcaaga gcgaagtcgc gcacgaactt gcactggttg
      721 gccgaggcga tgtccttggt gggcgtgaag tggtcgagga cgaacaccac cctgtcggga
      781 tgggcaaggg ccggcctgtc agcctttctg aactcctgga tggcgatcgg cgccgttatg
      841 tcgttcgcca gggccacgtc caccgtgacc cgcacgatct cccccgtccg gaccgtctcc
      901 atgcccgcgt gggccgcgat gatcttctcg gcgatggtca tgcccatggc accaccctca
      961 gaggctcggg gtcttcatgg ccttcctgcc cttgaggaac tccatgcggt tcatcgcgtt
     1021 cacgagggcc ttggcgcacg cgacgaggat gtccgggtcc gagccctggc cgaccgccgt
     1081 cttgcccttg tcctccagct cgatggtcac ctctccctgg gcgtcggtcc cgccggtgat
     1141 ggccttgatg gagaagctct t
//
LOCUS       DAOSCR010000758         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_83683_length_1155_cov_4.412727, whole genome shotgun sequence.
ACCESSION   DAOSCR010000758 DAOSCR010000000
VERSION     DAOSCR010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83683_length_1155_cov_4.412727"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLT69_13400"
     CDS             <1..255
                     /locus_tag="PLT69_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ15481.1"
                     /translation="PFLFALTKDSTTDRVIGELSYPVYLSHLMIIPFFGVLYRPGQPV
                     TTVAAVVFATLMVSLLAIFVVQVPVDRYRGRRLANSKAVP"
     gene            387..>1155
                     /locus_tag="PLT69_13405"
     CDS             387..>1155
                     /locus_tag="PLT69_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15482.1"
                     /translation="MRAMRARGEKRATARAWPQGTQRLLWCALIAACAFALYLQTAGF
                     SFVEYDDPAYVTQNPHVAEGLTWKSVAWALTSVYGSNWHPLTWLSHMLDIELFGMNAG
                     MHHLVNVFFHAANGVLLFLALEAMTGYRARSAVVALLFTVHPLHVESVAWVAERKDVL
                     STTFWMLAMAAYASYARNPSKARYALVALAFALGLTAKPMLVTLPFVLLLLDFWPLGR
                     VSDAVLGRAPGVSGGGAAGRAPLTRLDGEKIPLVILSA"
BASE COUNT          183 a          395 c          359 g          218 t
ORIGIN      
        1 cccttcctct tcgcgttgac gaaagacagc accacagacc gcgtcatcgg cgagctctca
       61 taccccgtct acctgtcaca cctcatgatc atcccgttct tcggggtgct gtacaggccg
      121 gggcagccgg tgacgaccgt ggcggccgtc gtcttcgcca ccctcatggt ctcgctgctc
      181 gcgatcttcg tcgtgcaggt gcccgttgac cgctaccggg gcagacgact ggcgaattcc
      241 aaagccgtac cctgaaagac gtacaccagc gtgaacatgg tcacacgagc gaacataccc
      301 ttcagcaatc aggttcccgg cgtatcggcg ctcggccttg caggatgcgg gcacggccga
      361 ggcgggcatg ggctttgggg gtgcctgtga gagccatgag agcccgggga gaaaagaggg
      421 cgacagccag ggcatggcct caaggaacgc agaggctgtt gtggtgcgcc ctcatcgctg
      481 cctgtgcgtt tgcgctgtac ctgcagaccg cgggtttctc cttcgtggag tacgacgacc
      541 cggcctacgt gacccagaac ccccacgtcg cagaaggtct cacgtggaag tccgtcgcct
      601 gggcgctcac ctccgtctac ggctcgaact ggcacccgct gacctggctc tcgcacatgc
      661 tcgacatcga gctcttcggg atgaacgcgg gcatgcacca cctcgtgaac gtgttcttcc
      721 acgcagcaaa cggcgtcctg ctcttcctgg cgctcgaggc catgaccggg taccgggcga
      781 ggagcgccgt ggtggcgctg ctcttcacgg tccacccgct ccacgtggag tctgttgcct
      841 gggtggccga acgcaaggac gtgttgagca ccacgttctg gatgctcgcc atggccgcgt
      901 acgcatcgta cgcgagaaac ccctcgaagg cgaggtacgc gctcgttgcg ctggccttcg
      961 cgctcgggct cacggccaag cctatgctcg tgaccctacc cttcgtgctg ctcctgctcg
     1021 acttctggcc cctcggccgc gtttccgacg cggttttggg acgtgccccc ggcgtttccg
     1081 gagggggcgc ggcgggacgc gcacccctca cacgcctgga cggcgagaag atccccctcg
     1141 tgatcctctc ggcgc
//
LOCUS       DAOSCR010000759         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_84290_length_1150_cov_2.020091, whole genome shotgun sequence.
ACCESSION   DAOSCR010000759 DAOSCR010000000
VERSION     DAOSCR010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84290_length_1150_cov_2.020091"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1150)
                     /locus_tag="PLT69_13410"
     CDS             complement(<1..>1150)
                     /locus_tag="PLT69_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ15483.1"
                     /translation="MVIYPEGTFYQYVKPEDVPEIIEEHIIKGRPVKRLMYKEPVTEK
                     TIPHMLDIPFFGKQKLIALKNRGLIDAEKIDEYIARDGYMGAAKALTQMTPDEVIEEV
                     KKSGLRGRGGGGFPTGLKWSFARKSPGDVKYILCNADEGDPGAFMDRSILEADPHAVL
                     EGMIIGGYAIGANEGYIYCRAEYPLAIKRLTIAIDQAKEYGLLGRNILGTDYSLDIFI
                     YQGAGAFVCGEETALMNSIEGKRGMPRPRPPFPATKGLWQKPSVLNNVETFANIPQII
                     LNGGDWYASMGTERSKGTKVFALTGAVNNVGLVEVPMGTPIGDIIFDIGGGIKKGKQY
                     KAAQLGGPSGGCIPAEALNTPTDYEEIVKLGAIMGSGGLIVMDEDTCMV"
BASE COUNT          169 a          365 c          372 g          244 t
ORIGIN      
        1 caccatgcag gtgtcctcgt ccatgacgat gagcccgccc gagcccatga tcgcgccgag
       61 cttcacgatc tcctcgtaat cggtcggcgt gttcagggcc tccgccggga tgcacccacc
      121 ggacgggccg ccgagctggg ccgccttgta ctgcttgccc ttcttgatgc cgccgccgat
      181 gtcgaagatg atgtcaccga tcggggtgcc catgggcacc tcgacgagac ccacgttgtt
      241 caccgcgccc gtgagtgcga agaccttggt gcccttggac cgctcggtac ccatggaggc
      301 gtaccagtcc cccccgttga ggatgatctg cgggatgttg gcgaaggtct ccacgttgtt
      361 gagcaccgag ggcttctgcc agaggccctt ggtcgcgggg aacgggggac gcggcctcgg
      421 catgccgcgc ttgccttcta tggagttcat gagcgccgtc tcctcgccgc agacgaaggc
      481 gccggctccc tggtagatga agatgtcgag gctgtagtct gtcccgagga tgttcctgcc
      541 gagcaggccg tattccttgg cctggtcgat ggctatggtg agccgcttga tggcgagcgg
      601 gtattcggcg cggcagtaga tgtagccctc gttcgcgccg atggcgtatc cgccgatgat
      661 catgccctcg agcacggcat gggggtcggc ctcgaggatg gagcggtcca tgaacgcgcc
      721 ggggtcgccc tcgtccgcgt tgcagaggat atacttcacg tcaccgggcg acttgcgggc
      781 gaaggaccac ttgagccccg tggggaaccc gccgcccccc ctgccacgca gacccgattt
      841 cttgacctcc tcgatcacct cgtcaggggt catctgggtg agggccttgg ccgcgcccat
      901 gtacccgtcg cgcgctatgt actcgtcgat cttctcggcg tctatgagcc ccctgttctt
      961 gagcgcgatg agcttctgct tgccgaagaa cgggatgtcg agcatgtggg ggatggtctt
     1021 ctcggtcacg ggctccttgt acatgagcct cttgaccggg cgtcccttga tgatgtgctc
     1081 ctcgatgatc tcgggcacgt cttccggctt cacgtactgg tagaacgtcc cctcggggta
     1141 gatgaccatg
//
LOCUS       DAOSCR010000760         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_85609_length_1137_cov_2.811460, whole genome shotgun sequence.
ACCESSION   DAOSCR010000760 DAOSCR010000000
VERSION     DAOSCR010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85609_length_1137_cov_2.811460"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="cfbD"
                     /locus_tag="PLT69_13415"
     CDS             complement(<1..850)
                     /gene="cfbD"
                     /locus_tag="PLT69_13415"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HOJ15484.1"
                     /translation="MYTLRDLDADVVLMHGPAGCGFMAARRLEEAGVRVMTTGMRESD
                     LIFGAEEKLIRILRSIDDRFRPRLIGVVGTCASMIIGENLDGAVKKAGVRATVLPVDI
                     HGCSGPNTTGAIAALEAAAAHRLITYEERDRQKRLLERATLIERERGLTSKSYLEPHP
                     GTTKLSAAQAIVRTLRRGGKVAVALNAKKETAYGFADVLRAVEHASSEVGGETYHIGN
                     LDPNIGLPRIRRYASDILRDLKEADVEVEITGGLDEYAVSGEKAAEALARCDADLRVI
                     VGVPHTV"
     gene            complement(892..>1137)
                     /locus_tag="PLT69_13420"
     CDS             complement(892..>1137)
                     /locus_tag="PLT69_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15485.1"
                     /translation="ECLDAYGCRDIGVAIEPVSLRVCEKLDLLAVRSAVEEHPAVTGL
                     YILPPGGGCEGFTAIGSIDEVKERHEVMLWCTKEGYI"
BASE COUNT          178 a          400 c          335 g          224 t
ORIGIN      
        1 gcaccgtgtg cgggacgccg acgatcacgc gaaggtcggc gtcgcatcgc gcgagcgcct
       61 cggccgcctt ctccccgctc acggcatact cgtccagccc gccggtgatc tccacctcga
      121 catcggcctc cttcaggtct cgaaggatgt ccgacgcgta cctgcggatg cgggggagtc
      181 cgatgtttgg atcgagattg ccgatgtgat aggtctcgcc gcccacctcg gagctcgcat
      241 gctcgacggc tcgcagaacg tcggcgaacc cgtatgcggt ctcttttttt gcattgaggg
      301 ccacagccac cttccctccg cgccgcagcg ttcgcacgat ggcctgggcg gcgctcagct
      361 tggtcgttcc gggatgcggc tccagatagc tcttggacgt gaggcctctc tccctctcga
      421 tcaaggtggc ccgttccagc agcctcttct gccggtccct ctcctcatac gttataagtc
      481 gatgcgcggc agcggcctcc agcgcggcga tggccccggt ggtgttggga ccggagcagc
      541 cgtgtatgtc gaccggcagc acagtagctc ggacgccggc cttcttcacc gcaccgtcaa
      601 ggttctcgcc gatgatcatc gatgcgcatg tgccgaccac gccgatcagc cttggacgga
      661 aacggtcgtc tatcgacctc aggattcgga tgagcttctc ctcggcgccg aagatcaggt
      721 cgctctcccg catgcccgtc gtcatcacgc gaacgcccgc ctcctccagc ctccgtgccg
      781 ccatgaaccc gcagcccgcc ggaccgtgca tcaggacgac atcggcgtcg agatcgcgca
      841 gcgtgtacat ggccgcgatg atcgggttgg gcctcggatg aagtatgtcg ttcatatgta
      901 tccctccttg gtgcaccaca gcatgacctc gtgtcgttcc ttcacctcat ctatggagcc
      961 gatcgccgtg aagccctcgc accctccccc cgggggcagg atgtacaggc cggtcaccgc
     1021 gggatgctcc tctaccgcgg acctcacggc caagaggtcg agcttctcgc acacccttag
     1081 gctgacgggt tcgatagcaa ccccgatgtc acggcagccg taggcgtcca ggcactc
//
LOCUS       DAOSCR010000761         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_85924_length_1134_cov_3.607044, whole genome shotgun sequence.
ACCESSION   DAOSCR010000761 DAOSCR010000000
VERSION     DAOSCR010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85924_length_1134_cov_3.607044"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLT69_13425"
     CDS             complement(<1..1060)
                     /locus_tag="PLT69_13425"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ15486.1"
                     /translation="MDSRENPKREIGATRTLQNFSGAVEHVKFHHSQTLYTVLRVRAK
                     APLDSSVTVIAHMPMPRTGDLFRFTGTWETHPVYGEQFHALSYEAAPPVTVEGIRAYL
                     ASEVEEIGPGLAGRVVEAFGLDTLRVIEEAPERLESVPGIGPKRARAITSAWGSRRAT
                     HDVLVFLYSQGLSPALAARVVNHYGDRAAEVVRTDPYRLALDIPGVGFLTADRIAMNT
                     GIPRDAPERLKAGVLYTLEAAAARGHAFLPRRALCHEAARLLNVDAHAAEATCDSLGE
                     ALLVAMEPDPGGSRGKDAVYLPGILNAEREAARLLACIAHAPKRPLGIDPSTAVAWME
                     ASRGVSLSERQRQAVRMAA"
BASE COUNT          161 a          393 c          394 g          186 t
ORIGIN      
        1 tcgcggccat ccgtaccgcc tgcctctgcc tttccgacag gctcacgccc ctcgacgcct
       61 ccatccacgc caccgccgtc gaggggtcga tgccgagcgg cctcttgggt gcgtgcgcta
      121 tgcatgcgag caggcgcgcc gcctctctct cggcgttgag gatgccgggg aggtagacgg
      181 cgtccttgcc cctcgagccc ccagggtccg gctccatcgc gacgaggagc gcctctccga
      241 gggaatcgca ggtggcctcg gcggcgtgtg catccacgtt cagcaggcgc gccgcctcgt
      301 ggcagagcgc acggcggggg aggaacgcgt ggcccctggc ggcggccgcc tcgagcgtgt
      361 agagcacgcc cgccttgagg cgctcgggcg catcacgggg gatgcccgtg ttcatggcta
      421 tgcggtccgc cgtgaggaac cccactcccg gtatgtccag ggcgaggcgg tagggatccg
      481 ttctgacgac ttccgccgcc cgatccccgt agtggttcac cacccgtgcc gcgagggctg
      541 gagagaggcc ctggctgtag aggaagacga gcacgtcgtg ggtcgccctg cgcgaccccc
      601 acgccgatgt gatggcacgc gccctcttgg gcccgatccc cggcacgctc tccagacgct
      661 cgggggcctc ctcgatcacg cgcagggtgt ccagcccgaa cgcctcgacg acccggccgg
      721 caagtcccgg cccgatctcc tccacctcgg aggcgaggta cgcgcgtata ccctcgacgg
      781 tcacgggcgg cgccgcctcg taggacaggg cgtgaaactg ttccccgtag acggggtgcg
      841 tctcccatgt cccggtgaag cggaagaggt cgcccgtgcg cggcatgggc atgtgggcga
      901 tcacggtgac ggaggagtcc aggggggcct tggccctcac gcggagcacg gtgtaaagcg
      961 tttgggaatg gtggaacttc acgtgctcca ccgccccgga gaagttctga agggtccttg
     1021 tggcaccgat ttcgcgcttg gggttttctc gactatccat ggtgaaaccg cttcactggg
     1081 gcccccgcag gcgtgcgggc ctctcgagga cgtccaacaa ccccttggcg cagt
//
LOCUS       DAOSCR010000762         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_86130_length_1132_cov_3.223770, whole genome shotgun sequence.
ACCESSION   DAOSCR010000762 DAOSCR010000000
VERSION     DAOSCR010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86130_length_1132_cov_3.223770"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..862)
                     /locus_tag="PLT69_13430"
     CDS             complement(29..862)
                     /locus_tag="PLT69_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ15487.1"
                     /translation="MALVRHRVAFAGLVILLGLIVMAAFAPVLAPYDPNEQDLYQVLQ
                     GPSRQHLLGTDDVGRDLLSRVIYGSRVTLAMGILSTLFAAVIGILAGLVAGYKGGIWD
                     AVIMRITDTFMCMPALVLALVIVAALGPGMKSVVISITVLAWTGFARITRGQVMIVRE
                     LPYVEAAHALGASGYRIMFRHLLPNVMAPLIVTATISVGMNIMLESAVSFLGIGVQQP
                     TPSWGNELRIGYSYLEIVPLFSIAPGLMITLAVLAFNFLGDGLRDALDPRLRGEEKAG
                     S"
     gene            complement(922..>1132)
                     /locus_tag="PLT69_13435"
     CDS             complement(922..>1132)
                     /locus_tag="PLT69_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ15488.1"
                     /translation="WGYVVAGAFIVEYMFAIPGLGRMGVNAIFANDFPVIQATLVMVA
                     VNILVVNLLVDLLYAYLDPRVRVQR"
BASE COUNT          228 a          358 c          372 g          174 t
ORIGIN      
        1 ttccttacct cgagcagcat ggccggctct agctccccgc tttctcttcc ccgcgcaggc
       61 ggggatccag ggcgtcccgg agaccgtctc ccaggaaatt gaaggccagg accgcgagcg
      121 ttatcatcag cccgggcgca atggagaaca ggggcacgat ctccaggtat gagtagccga
      181 tcctcagctc gttcccccag ctcggcgtgg gctgctggac acctataccc aggaaggaca
      241 cggcgctctc cagcatgatg ttcatgccca cggaaatggt tgcggtcacg atgagcgggg
      301 ccatgacatt ggggaggagg tgcctgaaca tgatacggta ccctgaagcg cccagggcat
      361 gcgcagcctc cacgtagggg agctcccgca cgatcatgac ctggccccgg gtgattcgtg
      421 cgaaacctgt ccacgcgagc acggtgatgg agatgaccac gctcttcatg ccgggcccca
      481 aggcggccac gatgaccagg gccaggacca gggcgggcat gcacatgaag gtgtccgtga
      541 tgcgcatgat gacggcgtcc cagatcccgc ccttgtaccc tgccacgagc cccgccagaa
      601 tgccgatgac cgcggcgaac agggtggaga gtatccccat ggccagagtg acccgggagc
      661 cgtagatgac acgggagagc aggtcgcggc ccacgtcgtc cgtgccgagc aggtgctgcc
      721 gggaggggcc ctggaggacc tggtagagat cctgctcgtt cgggtcgtag ggggccagga
      781 caggcgcgaa ggccgccatg acgatcagtc cgaggagaat gacgagtccc gcaaacgcca
      841 cccgatgccg aacaagggcc attgccgtct ccatcaaaac ggagcggtga gccctcggtg
      901 catcatcccg tgtgcggccg ttcatcgctg caccctcacc cgcgggtcga ggtaggcata
      961 caggaggtcg accaggaggt tcacgaccag gatgttgacg gccaccatga cgagcgtggc
     1021 ctggatcacg gggaagtcgt tcgcaaagat cgcgttgacc cccatgcggc ccagtcccgg
     1081 gatggcgaac atgtactcga cgatgaaggc gccggccacc acgtatcccc ag
//
LOCUS       DAOSCR010000763         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_86251_length_1131_cov_2.902416, whole genome shotgun sequence.
ACCESSION   DAOSCR010000763 DAOSCR010000000
VERSION     DAOSCR010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86251_length_1131_cov_2.902416"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLT69_13440"
     CDS             <1..286
                     /locus_tag="PLT69_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15489.1"
                     /translation="SASLVRGKNSWRKALDRLLTGIRAGVSLLVALSSKSRSCTGEGD
                     IDGGSSGVSAVMASESLAGEGTRRPLIFIAHSFYGGISAVRLTMITTKGE"
     gene            819..>1131
                     /locus_tag="PLT69_13445"
     CDS             819..>1131
                     /locus_tag="PLT69_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ15490.1"
                     /translation="MEKKDLQRLSLEKPSGGATRPAGRTLLAALAAGAACVAAAALVW
                     YFSTRSTEVKVASVARMYPSQLVSELEASGYVVAQRKADVAAKVTGRITEMLVREGSV
                     VR"
BASE COUNT          200 a          325 c          399 g          207 t
ORIGIN      
        1 ctcggcctcg ctcgtgcggg ggaagaactc gtggagaaag gccctggatc gcctgctgac
       61 gggtattcgc gccggtgtga gcctcctggt cgcgctctct tcgaagagca ggtcctgtac
      121 gggggagggt gatatcgatg ggggttcttc tggtgtctcc gcggtcatgg cctcggagtc
      181 tcttgccggc gagggaacca gacggccgct gatcttcatc gcacactcct tttacggtgg
      241 gatatcggct gtgagactga cgatgataac gacgaaagga gaatgacggt gacgttgtgc
      301 ggtgaggcct cgaaccttcg cgcaaccggc ttcatggtcg gttgtcaatg cctggcggca
      361 tggtccgtgg ggcatgccga ccccagttct gaatgccgtc gcatggtcgc gaccacctcg
      421 gcccgatcgc tgtacacccg cctgcaccaa cctttcggca agatatgctc gatttgttct
      481 tttcaccatt cgatcgaaaa atgcaagaga gaaaatgcgc ctatgcgaaa tttccctccg
      541 ccgttccacc aagcgggagg aggtgaggcg gccgacccct cgtggccggg gacgtgctcc
      601 ccggctccat gactggcctg catcgtttgt cgaggacgaa aggggccgcc ttcccctgtg
      661 ttcacgagat gcggggtgcg ggagggcgtg tgggatgacg gcacacggag agaacggggg
      721 gaaggccttg ccgcctgcct gcatgagaat gcgaatgcgg gagggagggg gtctgtgata
      781 ggatggtgcg gcaggcacga acggggagaa gcccaggggt ggagaagaag gatcttcaga
      841 ggctttcact ggagaaaccc tccggaggcg cgacacggcc cgcggggagg accctgctcg
      901 ccgctctcgc ggccggtgcg gcgtgcgtcg ccgccgcggc gctcgtatgg tacttctcca
      961 cgaggagcac ggaggtcaag gtcgcctcgg tcgcccgcat gtacccatcc cagctcgtat
     1021 cggaactgga ggcgagcggc tacgtggtcg cccagcgcaa ggccgacgtg gcggccaagg
     1081 tcaccggccg catcaccgag atgctggtcc gcgaagggag cgtcgtcagg c
//
LOCUS       DAOSCR010000764         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_86961_length_1125_cov_2.551402, whole genome shotgun sequence.
ACCESSION   DAOSCR010000764 DAOSCR010000000
VERSION     DAOSCR010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86961_length_1125_cov_2.551402"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLT69_13450"
     CDS             <1..492
                     /locus_tag="PLT69_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ15491.1"
                     /translation="GRTSRARQLARDTRARVERVHASTSGAYRPRVLVCVGNNMGSEG
                     ITDIFVAGKGSFHHELLLLAGARNAFAGQGPYRGVSLEAILVMDPDVIIDMVPEEGMR
                     PASRREILSSWKRLSALGAVRHDRVYVMAKDYAVIPGPRFVDLLEDMADVIHQKGAGN
                     GRP"
     gene            479..>1125
                     /locus_tag="PLT69_13455"
     CDS             479..>1125
                     /locus_tag="PLT69_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ15492.1"
                     /translation="MAGRETVIEAQRLSFRAGRRSILEGVSFHAAAGETVCIIGPNGA
                     GKSTLLKCLAGINETSAGALTICGRPLASYSRRSLARILSYVPQAYGHELPFFVHDFV
                     MMGRYAHIGPLSLPGKADRDAVEHAMATTGTIALADRHMATLSGGELQRVFIAAALAQ
                     QTCVMLLDEPTTFLDPKHRFEIHGLLMKISRDHGVTTVIATHDLAFALQSADRIIA"
BASE COUNT          205 a          369 c          361 g          190 t
ORIGIN      
        1 gggcgaacct cgagggcccg ccagctcgca cgtgacacca gggcgagggt cgagagggtc
       61 cacgcctcca cgagcggcgc ataccggccg agggtgctcg tatgcgtggg caacaacatg
      121 gggtctgagg gcataacgga cattttcgtt gcgggcaagg gatcgttcca tcacgagctc
      181 ctcctcctcg caggcgccag gaacgccttt gccgggcagg gcccgtaccg gggggtctct
      241 ctcgaggcga tcctggtcat ggatcccgat gtcatcatag acatggtccc cgaggagggc
      301 atgaggcccg cttcaaggcg ggagatcctc tcgtcgtgga agaggctctc tgccttgggg
      361 gccgtcaggc acgaccgggt atacgtaatg gccaaggatt acgcggtgat cccaggtcca
      421 cggttcgtgg atctgctcga ggacatggcc gacgtgatcc accagaaagg ggcgggaaat
      481 ggccggccgt gagaccgtga tcgaagcgca gaggctcagc tttcgcgcag gccgccggag
      541 tatcctcgaa ggggtctcgt tccatgccgc cgcgggcgag acggtatgca tcataggccc
      601 caacggagca gggaagtcca ccctcctgaa gtgtcttgcg ggcatcaacg aaacctcagc
      661 cggcgccctg acgatctgcg gacgacctct cgcgtcctac tcgaggcgct ctctcgcgag
      721 gatcctgagc tacgtgccgc aggcctatgg ccacgagctg ccgtttttcg tccacgactt
      781 cgtgatgatg ggacgctacg cccatattgg ccccctatcc cttcccggca aggcagaccg
      841 tgacgccgtc gagcatgcca tggccacgac gggcaccatc gcgcttgcgg acaggcacat
      901 ggccaccctg agcgggggcg agctgcagcg cgtgttcatc gcggcggccc tcgcgcagca
      961 gacctgcgtc atgctcctgg acgagcccac cacctttctc gaccccaagc accggttcga
     1021 gatccacggg ctcctcatga agataagccg ggatcacggc gtgaccaccg tgatcgccac
     1081 gcacgatctc gccttcgcac tccagtcggc ggaccgaatc atcgc
//
LOCUS       DAOSCR010000765         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_89017_length_1107_cov_3.488593, whole genome shotgun sequence.
ACCESSION   DAOSCR010000765 DAOSCR010000000
VERSION     DAOSCR010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89017_length_1107_cov_3.488593"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLT69_13460"
     CDS             complement(<1..851)
                     /locus_tag="PLT69_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15493.1"
                     /translation="MRKNVMCDEELYRVVFETAGTAIIVIDEDTTILLANSHFEKLSG
                     YSKEELEGRMSWTAFIAAEDVGRMKEYHEARRKDPGSAPQQYEFVFVDRSGGKRDILL
                     NVSLIPGTGRSVASCMDITERKEAERFLKKSEQNYRNILESIQEAYYEVDLEGNFTFF
                     NARAWQELGYPEGRLLGMNFRQYIHPDDVDKVFEVYHKVFVTGRPEGQVTFTVITHEG
                     KAMPVETSVSLRKDETGRVVGFKGVVRDVSVRAAYEAALRESEERYRLLAENSSDVIW
                     TLSLEGV"
BASE COUNT          204 a          372 c          278 g          253 t
ORIGIN      
        1 aagaccccct cgagcgagag cgtccatatc acgtcggagg aattctccgc cagcagcctg
       61 tacctctcct cgctctccct gagtgcggcc tcgtatgccg ccctcaccga cacgtccctc
      121 acgaccccct tgaacccgac gaccctgcct gtctcgtcct tcctgaggga gaccgaggtc
      181 tccacgggca tggccttccc ctcgtgagtg atcacggtga acgtcacctg cccctcgggc
      241 ctgccggtca cgaacacctt gtggtacacc tcgaacacct tgtccacgtc atcgggatgt
      301 atgtactgcc tgaagttcat gccgaggaga cgtccctccg ggtaccccag ttcctgccac
      361 gccctcgcgt tgaagaaggt gaagttgccc tcgagatcca cctcgtagta cgcctcctgg
      421 atgctctcga ggatgttccg gtagttctgt tcgctcttct tgaggaaacg ttccgcttcc
      481 ttgcgctcgg tgatgtccat gcaggatgcg acgctcctgc cggtccccgg gatcagggaa
      541 acgttcaaga ggatgtccct cttgccgccc gagcgatcga cgaacacgaa ctcgtattgc
      601 tgaggggcgc tcccgggatc cttccttctc gcctcgtgat actccttcat gcgcccgaca
      661 tcctctgcgg cgatgaaggc cgtccagctc atcctgccct cgagttcctc cttcgagtac
      721 cccgagagtt tctcgaagtg cgagttggca agcaggatgg tcgtgtcctc gtctatcacg
      781 atgatggctg taccggcggt ctcgaacacc acccggtaga gctcttcgtc acacataacg
      841 ttcttcctca tcctgccctg gccctccacc atggatcagc tctcacggaa tcatccatcc
      901 atcgaccgag tcgaacgtcc cctcaccttc cgggcgctca tcccatgcac aaaccgtacc
      961 tgcagatcgg gaaatcacct tgcgatatca acctgttcgg cagatcccgc gacttcatga
     1021 gggccctctc acaaagaaag aggggttttt cgtaaggttc atgacaactt tagggggtta
     1081 gatcgtaaca ttttgaggag cagtttg
//
LOCUS       DAOSCR010000766         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_89021_length_1107_cov_3.163498, whole genome shotgun sequence.
ACCESSION   DAOSCR010000766 DAOSCR010000000
VERSION     DAOSCR010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89021_length_1107_cov_3.163498"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..>1107)
                     /locus_tag="PLT69_13465"
     CDS             complement(338..>1107)
                     /locus_tag="PLT69_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOJ15494.1"
                     /translation="GPQRSYLEEAGSDPSRLRIAFTTRSPLGTPVDPECTNAVTDAAR
                     LLSELGHDVEEDEPDIDGHALAKSYFTMYFGEVAADIAEMEQVLARKVTHKDVEEPTW
                     SLKLLGDAFSSGEFVLAVRRWNTFARSMAAFHQRYDLYLTPTVAAPPVRIGELKPKPA
                     EETAMKIVNALGLGRLIRMSGIADKLAVENLSKTPFTQLANLTGQPAMSVPLYWSETG
                     LPIGVQFVARFGDEATLFRLAGQLERARPWAEKRPAI"
BASE COUNT          171 a          355 c          357 g          224 t
ORIGIN      
        1 gcacctgcca gaagctcagc gtcgtgccgt agaagtctgt gcagggcttg tccgggaacg
       61 aaccggcggg cagctggaac atggtctgcg ccgggatgcg cgggtagcgg atcgtcgtgg
      121 ggacattgta gtcgtagtgc acgtgccacg gtttctgctc catcgtgaaa cctcctcctg
      181 tctcgattga taaaaaaggc gttttgttca ggccgccttt gggatgcgac cattcacgca
      241 cgagcaggcc tcagggccga aggccccgtg aacacctatc atcactcccg ccggggggcc
      301 gagatcccct tcggcgaaaa atcgggcctt cttcgcttca gatcgcgggc ctcttttccg
      361 cccacggtcg tgccctctcg agctggcccg cgagcctgaa cagggtggcc tcgtcaccga
      421 agcgggcgac gaactgcacc cctatgggca gcccggtttc gctccagtac agggggaccg
      481 acatggcagg ctgccccgtg aggttcgcca gctgggtaaa gggggtcttg gacaggtttt
      541 ccaccgcgag cttgtcggcg atgccgctca tgcgtatcag cctgccgagc ccgagcgcgt
      601 tcacgatctt catggccgtc tcttccgcgg gcttgggttt cagctcgcct atcctcaccg
      661 ggggcgcggc caccgtgggg gtgaggtaga ggtcgtacct ctggtggaac gctgccatgc
      721 ttcgcgcaaa ggtgttccac cgcctgacgg ccagcacgaa ctctcccgac gagaaggcgt
      781 ctcccaggag cttgagggac caggtgggtt cctccacgtc cttgtgagtg accttcctcg
      841 cgaggacctg ctccatctcc gcgatgtccg ccgcaacctc cccgaagtac atggtgaagt
      901 agctcttcgc gagggcgtgg ccgtcgatgt ccggctcgtc ctcctccacg tcgtgcccca
      961 gctcggacag cagtctcgcc gcatccgtga cggcgtttgt gcactcgggg tctacggggg
     1021 tgcccagggg cgatctcgtc gtgaaggcta tgcggagcct tgacgggtct gatcccgcct
     1081 cctcgaggta tgacctctgg gggcctg
//
LOCUS       DAOSCR010000767         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_89152_length_1106_cov_3.163654, whole genome shotgun sequence.
ACCESSION   DAOSCR010000767 DAOSCR010000000
VERSION     DAOSCR010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89152_length_1106_cov_3.163654"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLT69_13470"
     CDS             complement(<1..519)
                     /locus_tag="PLT69_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ15495.1"
                     /translation="MARFAVQGGYVIKSAHDVLENGYVIVDGQTIADVTHELPPDIRE
                     VLGGPHDIVIPGLVNTHTHAPMTLFRGMADDLPLMTWLREHIFPAEARHVDREFVYVG
                     SLLAVWEMVRSGTTAFCDGYFYEEEVGRAAKEIGVRAWIGEGILQFPTPSLSDPARTI
                     EHSREIIERWKDD"
     gene            complement(534..>1106)
                     /gene="recR"
                     /locus_tag="PLT69_13475"
     CDS             complement(534..>1106)
                     /gene="recR"
                     /locus_tag="PLT69_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ15496.1"
                     /translation="RRLPGIGEKTATRLALAILADKGDLSRCLVQALEAVRREIRLCP
                     ECFNLTDGDLCAVCADVSRDRSLICVVEEPSDVLSIESSNVYKGLYHVLHGLVSPLSG
                     IGPNDIRLPELMERLKKYQDQVRELIVATNPSVDGEGTFHHICSLVERSGVAVVVSRI
                     ASGIPMGGELKYMDQVTIASALTYRRSTQC"
BASE COUNT          177 a          378 c          337 g          214 t
ORIGIN      
        1 gtcgtccttc cagcgctcga tgatctccct gctgtgttct atggtcctgg cggggtctga
       61 cagcgacggc gtcgggaact ggaggatccc ttccccaatc caggcccgca ccccgatctc
      121 cttggccgcc ctgcccacct cctcctcgta gaagtagccg tcgcagaacg cggtcgtgcc
      181 gctgcgcacc atctcccaga cggcgaggag cgagcccacg tacacgaact cccggtcaac
      241 gtgtctcgcc tccgcgggga agatgtgctc cctgagccat gtcatgagcg ggagatcgtc
      301 ggccatgccc ctgaagagcg tcatgggggc gtgcgtgtgg gtgttgacga gcccggggat
      361 cacgatgtca tgggggcctc ccaggacctc gcgtatgtcg ggggggagct cgtgcgtgac
      421 atcggctatc gtctgcccgt cgacgatgac gtatccgttc tcgagtacgt cgtgggcgct
      481 tttgatcacg tacccgccct gcacggcgaa ccttgccatt gacgctcccc ctttcagcac
      541 tgtgtgctcc tgcggtacgt aagggcgctc gcgatggtca cctggtccat gtacttcaac
      601 tctcccccca tggggatccc cgaggcgatc ctgctcacca ccacggcgac gccggatctc
      661 tccaccaggg agcagatgtg gtggaaggtg ccctcaccgt cgacggacgg gttcgtggcc
      721 acgatgagct ccctcacctg atcctggtat ttcttcagcc tctccatgag ctcgggcagc
      781 cgtatgtcgt tcggccctat gccggacagc ggcgacacca gcccgtgcag gacgtgatag
      841 aggcccttgt acacgttcga ggactcgatg gacagcacgt cgctcggttc ctcaaccacg
      901 catatcagcg acctgtcgcg cgagacgtcc gcgcacacgg cgcacagatc cccgtcggtc
      961 aggttgaagc actccgggca gagcctgatc tcccgcctta ccgcctcgag ggcctgcacg
     1021 aggcagcggg aaagatcccc cttgtctgcg aggatcgcca gcgcaagcct ggtagcggtc
     1081 ttctcgccga tgccgggcag gcgcct
//
LOCUS       DAOSCR010000768         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_89404_length_1104_cov_3.219256, whole genome shotgun sequence.
ACCESSION   DAOSCR010000768 DAOSCR010000000
VERSION     DAOSCR010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89404_length_1104_cov_3.219256"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1104)
                     /gene="tig"
                     /locus_tag="PLT69_13480"
     CDS             complement(<1..>1104)
                     /gene="tig"
                     /locus_tag="PLT69_13480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOJ15497.1"
                     /translation="RGFRKGKAPRHILRTYYGDYIDGELSRKLVREKFEEAVREKDLF
                     VVSMPEFDNAQPKENEEFSFTATFDVKPEVDPAVYTGFDLEKPRVEVSDGDVDAVIER
                     LRQTFAEVKEVEDPAYGAAEGDYVVVNVSSESDTSLDRERMTVEAGARSAFPGLEHAV
                     TGMKVGDTKEVEVTFGDDHFLEDKRGKTFTLKIQVLAVRNRVLPDVDDEFARMVHQGA
                     QNVEELRKAIREDLQSRVESEARLVLERQVEEKLLEANTFDVPESMVRLQAIMMLQGM
                     SQRLASQGVRLQDVYPDADQLRDESMASAERLVKTSLLVEAIAKKHGIEATDEDVEKE
                     MAKLAEKYSMSVEAVKGRFEEQGRIDEMRYGILE"
BASE COUNT          166 a          399 c          307 g          232 t
ORIGIN      
        1 ctcgaggatc ccgtagcgca tctcgtcgat gcgcccctgt tcctcgaacc tgcccttcac
       61 cgcctccacg ctcatggagt acttttcggc aagcttggcc atttcctttt ccacgtcctc
      121 gtccgtcgcc tctatgccgt gcttcttcgc gatggcctcg acgaggagcg acgtcttcac
      181 caggcgttcc gccgaggcca tggactcgtc cctgagctga tccgcgtcgg ggtagacgtc
      241 ctggagcctc acgccctgcg aggcgaggcg ctgcgacatc ccctggagca tcatgatggc
      301 ctggagcctg accatggact ccgggacgtc gaaggtgttc gcctccagga gcttctcctc
      361 cacctggcgc tccagcacca agcgcgcctc ggactcgacc ctcgattgga gatcctcccg
      421 gatcgccttt ctgagctctt ccacgttctg cgcaccctgg tgcaccatcc tcgcgaactc
      481 gtcgtccaca tccggaagca cccggttgcg cacggcgagc acctggatct tcagcgtgaa
      541 ggtcttgccc cgcttgtcct cgaggaaatg atcatccccg aaggtgacct ccacctcctt
      601 cgtgtccccc accttcatgc ccgtcacagc gtgctcgagc cccgggaagg cgctgcgggc
      661 gccggcctcc acggtcatgc gttcgcggtc gagcgaggtg tcggactccg aggacacgtt
      721 gaccaccacg taatcgccct cggccgctcc atacgccggg tcctccacct ccttgacctc
      781 ggcgaaggtc tgcctgaggc gctcgatgac ggcgtcgaca tccccgtcgc tcacctcgac
      841 cctcggtttc tcgaggtcga agccggtata gacggcaggg tccacctccg gcttcacgtc
      901 gaaggtggcc gtgaaggaga actcctcgtt ctccttgggc tgtgcattgt cgaactcggg
      961 catggacacg acgaagagat ccttctccct gacggcctcc tcgaatttct cgcggacgag
     1021 cttgcgggag agctcgccat cgatgtagtc gccgtagtac gtcctcagga tgtgcctcgg
     1081 ggcctttccc ttccggaacc ccct
//
LOCUS       DAOSCR010000769         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_89456_length_1104_cov_2.042898, whole genome shotgun sequence.
ACCESSION   DAOSCR010000769 DAOSCR010000000
VERSION     DAOSCR010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89456_length_1104_cov_2.042898"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1104)
                     /locus_tag="PLT69_13485"
     CDS             complement(<1..>1104)
                     /locus_tag="PLT69_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ15498.1"
                     /translation="IDYLVEHDEPLVEYAPEEHDYETRMIGQHVAGLVPDGATIQLGF
                     GRIPDAAARALGNRRGLSIVSETITNSVMELMKAGVVARAEPVAVSMCIGDAELFRFV
                     HKNPAVSMRSLAGLCDPQDLLGRPCFVAVNGAVEIDLTGQSCMNLSERGAYLGALGQP
                     LFHRMAQLSREGKAIIALRSTSRDTSISRIVPKFTDPRMGVFTTQSEVEYVVTEQGCV
                     NLFGKSIRERSLELITVAHPRFRSWLLGEAKRLCYIYPDQVMPPEDAVYPAEYEHVGV
                     FDGKEVFIRPARITDERAVQNLFYSMSNDDRFHRFLIQIKAFHHAQAQRMVNCDYRDT
                     FAMVAEDRTGGSTQVVAVAQICKDSCGDGPQEG"
BASE COUNT          187 a          361 c          346 g          210 t
ORIGIN      
        1 ccccttcctg tgggccgtct ccgcacgaat ccttgcagat ctgcgccacc gccacgacct
       61 gggtcgaccc gcctgttcgg tcctctgcga ccatggcgaa ggtgtccctg tagtcgcagt
      121 tgaccatgcg ctgcgcctgg gcgtgatgga acgccttgat ctggatgagg aagcggtgga
      181 acctgtcgtc gttgctcatg gagtagaaca ggttctgcac ggcccgttca tcggtgatcc
      241 gggccggcct gatgaacacc tccttgccgt cgaagacccc tacgtgctcg tactcggccg
      301 ggtagacagc atcctcgggg ggcatgacct ggtccgggta gatgtagcag agccgtttcg
      361 cctcgcccaa cagccatgag cggaacctgg gatgggcgac ggtgatgagc tcgagcgagc
      421 gctctcgtat ggacttcccg aagaggttca cgcagccctg ctcggtgacc acgtattcca
      481 cctcgctctg cgtggtgaac acgcccatcc gcgggtccgt gaacttcggc acgatcctcg
      541 atatgctcgt gtcgcgcgag gtggacctga gcgctatgat cgccttcccc tccctggaga
      601 gctgggccat cctgtggaag aggggctgcc cgagggctcc caggtacgcg ccgcgttcgc
      661 tcaggttcat gcacgactgc ccggtgaggt cgatctcgac ggcgccgttg accgcgacga
      721 aacacggcct gcccaagaga tcctgcgggt cgcacaagcc cgcaagggaa cgcatcgaca
      781 cggcggggtt cttgtgcacg aaccggaaga gctcggcatc gcctatgcac atggatacgg
      841 cgacaggctc agccctcgcg acgacacccg ccttcatgag ctccatgacc gagttcgtga
      901 tggtctccga gacgatcgac aacccacgcc tgttccccaa ggcgcgggcc gccgcatccg
      961 ggatcctgcc gaacccgagc tggatcgtgg caccgtcggg cacgagcccg gcaacgtgtt
     1021 gcccgatcat cctggtctcg tagtcgtgtt cctcgggtgc atactccacg agcggctcgt
     1081 cgtgttcgac gaggtaatcg atgt
//
LOCUS       DAOSCR010000770         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_90312_length_1097_cov_2.006718, whole genome shotgun sequence.
ACCESSION   DAOSCR010000770 DAOSCR010000000
VERSION     DAOSCR010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90312_length_1097_cov_2.006718"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /gene="ssrA"
                     /locus_tag="PLT69_13490"
     tmRNA           complement(<1..344)
                     /gene="ssrA"
                     /locus_tag="PLT69_13490"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(383..949)
                     /locus_tag="PLT69_13495"
     CDS             complement(383..949)
                     /locus_tag="PLT69_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOJ15499.1"
                     /translation="MDRIREIIEESAGLHGRLGELIPQIRAAARVMADALARGNKIMF
                     AGNGGSAADAQHLAAELVNRFLKDRRPLAGLALTTDTSVLTAVGNDFNFEEIFSKQVR
                     ALGRAGDVLVGISTSGGSRNVIRALMEAGSMGIARIALIGEGGAMRDHAETVIAVPSR
                     NTPRVQEVHILVGHILCELVENEFTHIV"
BASE COUNT          201 a          369 c          303 g          224 t
ORIGIN      
        1 gggaatcgaa cccccgtccg aaggccttca gccaaaggct cctacatgct tagtctgtgt
       61 attcaaaccc agacgcgccc ggcccacaga caggctctgc acgtcccgac ccccctttgg
      121 ttccctgccg gcccgagggg ctctgaccgc caggtttgcc cattctcatt tgacgcccgt
      181 acgcaggtca atgggcaggc ccgcgcagga cgtggccgct cttaggcagc cagtgcgtaa
      241 gaataatcgt cggcacttat tggttttctg cctgttttac gaggtagcag acctcggcat
      301 gctcctggta cctcaactgc ccccgtcgaa cccattacgc ccccttacgt agaatctata
      361 gtagtatcga tgatcgagta tgtcaaacga tgtgcgtgaa ttcgttctcg accagctcgc
      421 agaggatgtg ccccacgagg atgtggacct cctgcaccct gggcgtgttc cgggaaggaa
      481 ccgcgatgac tgtctccgcg tggtcccgca ttgccccgcc ctcgccgatg agcgctatcc
      541 tggcaatgcc catcgatccg gcctccatga gcgcgcggat gacattcctc gagccgccgc
      601 tcgtggagat tcccacgagc acgtcccctg ccctgcccag ggcccttacc tgcttggaaa
      661 agatctcctc gaagttgaag tcgttgccca ccgcggtgag aaccgaggtg tccgtggtca
      721 gcgcaagccc ggccaggggc cgacggtcct ttaggaaccg gttcacgagc tcggcggcaa
      781 ggtgctgggc atcggctgca gaaccgccgt tgcccgcaaa catgatcttg ttgccgcggg
      841 caagggcgtc ggccatcacc cgggccgccg cccgaatctg gggtatcagc tctcccaggc
      901 ggccgtgcag ccctgcgctc tcctcaatga tctctcggat gcggtccatt gcgctccctt
      961 gcctcgtaaa gggcattctc tgaaattacc agaccatata agcgagatcc acgtcgaaga
     1021 caagcccaat cgggccggtt cgcctcctgt gtcggcgacg aacggcctcg gtacacctat
     1081 acccttcgat ttaccat
//
LOCUS       DAOSCR010000771         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_90817_length_1092_cov_3.176471, whole genome shotgun sequence.
ACCESSION   DAOSCR010000771 DAOSCR010000000
VERSION     DAOSCR010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90817_length_1092_cov_3.176471"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLT69_13500"
     CDS             <1..199
                     /locus_tag="PLT69_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15500.1"
                     /translation="IDSTSISVSDSDPALGMCGLSIYLPEADEATQEDLSLYGELACN
                     RDRAEGGETWADLLSAVLLGR"
     gene            358..666
                     /locus_tag="PLT69_13505"
     CDS             358..666
                     /locus_tag="PLT69_13505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOJ15501.1"
                     /translation="MITVIATMRAKKGAEAEVEDALREMTGKVAGEEGTLEYVLHRSV
                     KDPCLFVMYERYRDKDALASHSSSPHFLKLLERIGPAIDGQPSIEILGEIARAHRTAT
                     "
     gene            complement(732..>1092)
                     /locus_tag="PLT69_13510"
     CDS             complement(732..>1092)
                     /locus_tag="PLT69_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOJ15502.1"
                     /translation="ARRPVDRHDPFLYHKTTRRRVYEEALAEAASYDDVVLYNHGGYV
                     TESTRANVVARIDGGLVTPPVDCGLLPGTFRQWMLENSLARERPITVQELLEAERVFL
                     VNSVRGMYPVVVDGHGA"
BASE COUNT          184 a          353 c          381 g          174 t
ORIGIN      
        1 gatcgacagc acctcgatct cggtctccga cagcgacccg gcgctcggga tgtgcggcct
       61 gtccatctac ctgccggagg ccgacgaggc gacccaggag gacttgtccc tctacggaga
      121 gctcgcgtgc aacagggaca gggccgaggg gggtgagacg tgggccgatc tcctctccgc
      181 ggtcctcttg ggcaggtagg gggggtttgg ggcggttgcc ttcggccgcg cgcaggatgt
      241 accgaacgtc ccgaagaacc cgattccctt gcgccgggca aggtcttcct ggtacccttg
      301 aagcatcgac ggcccctggt gcatgcgggg cctgaatcac gatcgagggg gggagcgatg
      361 atcaccgtga ttgcgaccat gagggcgaag aagggcgccg aggccgaggt ggaagacgcc
      421 ctgagggaga tgacgggcaa ggttgccgga gaggagggca ccctcgagta cgtgctccac
      481 cgctccgtga aggacccctg cctgttcgtc atgtacgaga ggtaccgcga caaggatgcc
      541 ctggcatcgc acagctcgag cccgcacttc ctcaagctcc tggaacggat aggaccggcg
      601 atcgacggcc agccctcgat cgagatcctc ggggagatcg cccgcgccca ccgcacagcc
      661 acctgagaaa acgaccctgc cgtcccccgt ccgggggcgg ccctgtccgc accacgggag
      721 gtccgggatc atcaggcgcc gtgtccgtcg accacgacgg ggtacatccc cctgaccgag
      781 ttcacgagaa agaccctctc ggcctcgagg agctcttgga cggttatggg ccgctcccgt
      841 gcgagggagt tctcgagcat ccactgcctg aaggtgcccg gcaggaggcc gcagtccacg
      901 gggggggtga cgagcccgcc gtcgatgcgg gcgaccacgt tggccctggt ggactcggtg
      961 acgtacccgc catggttgta cagcaccacg tcgtcgtaac tcgccgcctc ggcgagggct
     1021 tcctcgtaga cgcgcctcct cgtggtcttg tggtagagga acgggtcgtg cctgtccacg
     1081 ggtctgcgcg cg
//
LOCUS       DAOSCR010000772         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_91079_length_1090_cov_2.626087, whole genome shotgun sequence.
ACCESSION   DAOSCR010000772 DAOSCR010000000
VERSION     DAOSCR010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91079_length_1090_cov_2.626087"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLT69_13515"
     CDS             complement(<1..459)
                     /locus_tag="PLT69_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ15503.1"
                     /translation="MTGTIVFWVVVGIIVALVITVVGIYNSLVALRNQVRNAWSQIDV
                     QLKRRHDLIPNLVETAKGYMKHERETFDAVTKARAHAEAARGVPQIAQAEGRLTEAIS
                     RFMAVMENYPELKANQNFLAVQEELASTENRIAFARQAYNDQVLDFNDQAA"
     gene            complement(456..854)
                     /locus_tag="PLT69_13520"
     CDS             complement(456..854)
                     /locus_tag="PLT69_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOJ15504.1"
                     /translation="MNCPACTSNLVTLELNGVEVDYCFTCQGIWLDNGELERLLSMAE
                     GDEGMVSGLMPAAVSEKRRRCPVCRRVMEKVETKRQGGVLLDRCDRHGLWFDRLELRK
                     VLEMGCAPGVMSPLTRILDDMFAARGRGCV"
BASE COUNT          171 a          389 c          330 g          200 t
ORIGIN      
        1 cgcggcctgg tcgttgaagt cgagcacctg gtcgttgtag gcctggcggg caaaggcgat
       61 cctgttctcc gtggaggcga gctcctcctg cacggccagg aagttctggt tggccttgag
      121 ctcggggtag ttctccatca cggccatgaa ccgggagatg gcctccgtga gacggccttc
      181 cgcctgggcg atctggggaa cgcccctggc ggcctcggcg tgcgccctgg ccttggtcac
      241 ggcgtcgaag gtctcgcgct cgtgcttcat gtagcccttg gcggtctcca caaggttggg
      301 gatcaggtcg tgcctgcgct tgagctgcac gtcgatctgc gaccacgcgt tcctcacctg
      361 gttcctcagg gccaccagcg agttgtagat cccaaccacg gtgatgacga gcgccacgat
      421 gatcccgacc accacccaga agacgatcgt ccctgtcata cgcaccccct ccctcgagcc
      481 gcgaacatgt cgtcgaggat cctcgtaagc gggctcatca cacccggcgc acagcccatc
      541 tcaaggacct tcctcagctc gagcctgtcg aaccacaggc cgtggcggtc gcacctgtcc
      601 aggaggacgc caccctgtct tttcgtctcc accttctcca tcaccctgcg gcacaccggg
      661 caccgcctcc tcttttcgga gacagccgcg ggcatcaagc cggacaccat gccctcgtcc
      721 ccctccgcca tgctcaacag gcgctccagc tcgccattgt ccagccagat gccctggcag
      781 gtgaagcagt agtcaacctc caccccgttc agctccaggg tcaccaggtt gctcgtacag
      841 gcgggacagt tcatggcggc gctcccttcc cttgttgtcg gacttcggca ggtcgatctt
      901 gagacccctg gcgacacggt cgacggggcg tccgccgtgt ctggcccgcc acgggctcgt
      961 ggggagcgcg agcaggccac cccccggcca tgcgctcgtc gaggatgccc atgcacacga
     1021 gcagcagggg gctcacccgg tgcccggggc ccccacaggg attttatgcg tcaggcgatc
     1081 ggcggaggca
//
LOCUS       DAOSCR010000773         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_91191_length_1089_cov_3.376209, whole genome shotgun sequence.
ACCESSION   DAOSCR010000773 DAOSCR010000000
VERSION     DAOSCR010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91191_length_1089_cov_3.376209"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..>1089
                     /locus_tag="PLT69_13525"
     CDS             415..>1089
                     /locus_tag="PLT69_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15505.1"
                     /translation="MMAYQVFSIHGKGAMIEVRRVTGRGMLKEFVMLPWSARIYEKDP
                     AWVPPLLHDMYTMLDPRKGYFYQIGEAELFIAYQDGRPVGRISAHVNRLYEQRYDRDT
                     GFFGFFESIDDVGVARALFDTAEAWLTSRGKKVMNGPQSFSIYDSVGFEVEGAHRMPT
                     VGLFHFAPYYRELAEACGLKKCIDWHCFLVKKSDYDVYAPYLRDVKDRLLSSSEFTFK
                     RLDKRVM"
BASE COUNT          202 a          355 c          342 g          190 t
ORIGIN      
        1 gggggcatcc tccgcggcac ctcctgtgct cgtcgagggt ctccaatggc gagcgagcca
       61 ggggcaggga cccaccgccc gagtcccggc ctggttctcc ggcgactcat cgatacccac
      121 ctgcctcgtc gccagggcgc ggcccgagga gatccaggcg ctcgtgctcg acatcccggt
      181 gatccctgca gaaggtcccc cggtgacgac atcccagcag ggaacaggcg cgcctgcgac
      241 ccctcccctg agaagcgtgg aggtccggga ctggtggcct tgcactgcgg atcaaaggca
      301 tcgtccctgc aatccctacc cacaccaagc tgggagccct gtgggaagac gcctcgttcg
      361 catctcgtgc gggccgcgaa acgcgtcttc ttgcaagcgg ggataaatgg tgcgatgatg
      421 gcgtatcaag tattctccat ccacgggaag ggtgccatga tcgaggtgag gagggtgacg
      481 ggcaggggca tgctcaagga attcgtgatg ctcccttgga gcgcgcgtat ctacgagaaa
      541 gacccggcgt gggtcccccc cctgctccac gacatgtaca ccatgctcga ccccaggaaa
      601 ggctacttct atcagatcgg cgaggcggag ctcttcatcg cctaccaaga cggcaggccc
      661 gtgggtcgga tcagcgccca cgtgaaccgc ctctacgagc agcgatacga cagggacacc
      721 gggttcttcg gcttcttcga gtcgatcgac gacgtcgggg tcgcccgggc actgttcgac
      781 acggccgagg cctggctcac gtcgcggggc aagaaggtca tgaacgggcc ccagagcttt
      841 tccatctatg actcggtcgg cttcgaggtc gagggcgccc accgcatgcc cacggtgggc
      901 ctcttccact tcgcgcccta ctaccgggaa ctcgccgagg cctgcggctt gaagaagtgc
      961 atcgactggc actgtttcct cgtgaagaaa tcggactacg acgtctacgc cccgtacctc
     1021 agggacgtga aggacaggct cctctcttcg agcgagttca cgttcaagag gctcgacaag
     1081 cgcgtcatg
//
LOCUS       DAOSCR010000774         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_91305_length_1088_cov_4.126815, whole genome shotgun sequence.
ACCESSION   DAOSCR010000774 DAOSCR010000000
VERSION     DAOSCR010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91305_length_1088_cov_4.126815"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1088
                     /locus_tag="PLT69_13530"
     CDS             <1..>1088
                     /locus_tag="PLT69_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOJ15506.1"
                     /translation="VTHKTERGGVVLDIDDEASLLAHASRLQDAFAGIPHKLLVQRMA
                     PRGTELIVGARRDPVFGPVVLVGAGGILAELIRDTVVELAPVDRGRALAMLGRLKARA
                     LLEGFRGAPRLDVEAVSSVIEAVSRLVSERDDIVEVDINPLVVSTSGALAVDALVRLG
                     HEAHVPKKRPRTPEGGMEKFFEPGSIALLGASRSHGKGGNIILRNLVRAGFKGAILPI
                     NPSGHDILGMRSYKRLTDVPGPVDLAMIVIPKRAVREALADCAAKNVGAVILCTGGYA
                     DAGPEGAAEQKQLVSFARSHGMRLMGPNSIGVINPGKGLATSIVGLEPLKPGGVALVG
                     QSGVFSSGWGRWIAESAPFGVSKVACIG"
BASE COUNT          170 a          372 c          384 g          162 t
ORIGIN      
        1 cgtcacgcac aagacagagc gcggcggcgt ggtgctggac atcgacgacg aggcgtcgct
       61 cctcgcgcac gcctcccgac tgcaagacgc gttcgccggc ataccccaca agctcctcgt
      121 ccagcgcatg gcgcctcgtg gaacggagct catcgtgggc gcaaggcgcg acccggtctt
      181 cggccctgtc gtcctcgtcg gcgcgggcgg gatcttggcg gagctcatcc gggacaccgt
      241 ggtggagctc gcgcccgtag accggggccg ggcgctcgcc atgctcggcc gcctcaaggc
      301 cagggccctg ctcgaggggt ttcggggcgc gccgcgtctc gacgtcgagg cggtctcgtc
      361 cgtgatagag gccgtgtccc gcctcgtgtc tgaaagagac gacatcgtcg aggtggacat
      421 caacccactc gtcgtgtcga cctcgggcgc actcgcggtg gacgcgctcg tgcgcctcgg
      481 gcacgaggcc cacgtcccga aaaagcgccc acgtacgccc gaaggcggca tggagaagtt
      541 cttcgagccg ggctccatcg ccctcctcgg cgcgtcgagg agccacggca agggaggcaa
      601 catcatcctg agaaacctcg tcagggccgg gttcaagggc gcgatcctcc ccatcaaccc
      661 ctcgggccac gacatcctgg gcatgaggag ctacaagagg ctcaccgacg tgccgggccc
      721 ggtggacctc gccatgatcg tgatccccaa gcgggccgtc cgggaagctc tcgccgactg
      781 cgcggcgaag aacgtgggcg ccgtgatcct gtgcacgggc ggctatgcgg atgcaggccc
      841 cgagggtgcg gccgagcaga aacaactcgt ctccttcgcg cgttcgcacg gcatgcgcct
      901 catggggccc aacagcatag gggtcatcaa cccgggcaag gggctcgcga cctcgatcgt
      961 ggggctcgag cccctgaagc cgggcggcgt ggcgctcgtg ggccagtcgg gcgtcttctc
     1021 gtcgggctgg ggcaggtgga tcgcggagag cgcgcccttc ggcgtctcga aggtagcgtg
     1081 catcggca
//
LOCUS       DAOSCR010000775         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_91868_length_1084_cov_2.564626, whole genome shotgun sequence.
ACCESSION   DAOSCR010000775 DAOSCR010000000
VERSION     DAOSCR010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91868_length_1084_cov_2.564626"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLT69_13535"
     CDS             <1..207
                     /locus_tag="PLT69_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOJ15507.1"
                     /translation="DEPPSTKDVRGTNRCLIFVAKHPSRPRATIVSVIDNLTKGASGQ
                     AVQCMNLMFGLEETLGLDSLPLYP"
     gene            334..>1084
                     /gene="amrB"
                     /locus_tag="PLT69_13540"
     CDS             334..>1084
                     /gene="amrB"
                     /locus_tag="PLT69_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOJ15508.1"
                     /translation="MEGPVRKAVIAGSWYPGDPESLRRDIRRYLDSACVEPPEGKPVA
                     LVSPHAGYLYSGPVAAFAYKAEEGLSYTTVVVISPSHRVAFPSVSVWPSGAYETPLGR
                     IEVDEALCSELLEWPVFTDDTRPHAREHALEIQLPFLQVTLGSFKLCPLIMGSQDADV
                     CESVARALHEAVLSPEDTLVVASSDLSHFHRSATAERMDLAVAKYLESFDITSLSKAL
                     DRSEVEACGGGPILAAMIYAKLLGRTTSRVLR"
BASE COUNT          192 a          370 c          338 g          184 t
ORIGIN      
        1 gacgagcctc cgtccaccaa ggacgtgcgg ggcacgaacc ggtgtctcat cttcgtcgca
       61 aagcacccgt ccagaccccg cgccaccatc gtgagcgtca tcgacaacct gaccaagggg
      121 gcctcgggcc aggccgtaca gtgcatgaac ctcatgttcg gcctcgagga gaccttgggc
      181 cttgactcgc ttcccctcta cccttaatgt tcctggcaca acctatcacg ggcccaggct
      241 ccgcaaggag gaccaacggc gattcggggg cctgaggaac gccccacgac aacggggatt
      301 gcttgcgagg gcccggcctc tcggaggcgc accatggagg gaccggttcg gaaagccgtg
      361 atcgcgggga gctggtaccc cggcgatccc gagtccctga ggagggacat caggcgctac
      421 ctcgacagcg cgtgcgtcga gccgcccgaa ggcaagccgg tcgcgctcgt gtcgccccat
      481 gcgggatacc tctactccgg gccggtcgcc gcctttgcct acaaggcgga ggaaggcctt
      541 tcctacacta cggtggtggt cataagcccc tcgcacaggg tggcgttccc ctcggtgtcg
      601 gtgtggccga gcggggccta cgagacgccg ctcgggagga tagaggtgga cgaggccctc
      661 tgctccgaac tgctcgaatg gccggtgttc accgacgaca cccggccgca cgcacgggag
      721 cacgcactcg aaatacagct tccctttctc caggtcacgc tcggcagctt caagctctgc
      781 ccgctcatca tgggcagcca ggacgcagac gtgtgcgaat ccgtggcaag ggcgctgcac
      841 gaggccgtcc tttcccccga ggacaccctg gtggtcgcaa gctctgacct ttcccacttc
      901 catcgatcgg ccacggccga gcgcatggat ctcgccgttg ccaagtacct agagtcgttc
      961 gacatcacca gcctttccaa ggcgctcgat cgctcggagg tcgaggcctg cggcggcggg
     1021 ccgattcttg cggccatgat ctatgcaaaa ctcctgggca ggaccacatc tcgggtgctc
     1081 aggt
//
LOCUS       DAOSCR010000776         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_92781_length_1077_cov_3.311155, whole genome shotgun sequence.
ACCESSION   DAOSCR010000776 DAOSCR010000000
VERSION     DAOSCR010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92781_length_1077_cov_3.311155"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT69_13545"
     CDS             <1..283
                     /locus_tag="PLT69_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOJ15509.1"
                     /translation="KFLGGATVAELTPGLREDMRIDPKVQGVVVTGVASNSEAAGTGL
                     RPGDVVLSINGKPTRTIDDFKRVVAGLKGMKMSISLYRQGAVMNLTIIR"
     gene            300..752
                     /gene="dtd"
                     /locus_tag="PLT69_13550"
     CDS             300..752
                     /gene="dtd"
                     /locus_tag="PLT69_13550"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ15510.1"
                     /translation="MRTVVQRVSRASVKTSGVQVCSIDKGLCCLVGVEKGDGEGDLDY
                     TARKIVGLRIFEDEEGAMNLDVQTTGGDILLVSQFTLLADARKGRRPSFADAEDGAQA
                     RELFDALVAKVRGIFPGRVETGVFGAMMEVDIVNNGPVTILLDSRRRF"
     gene            complement(912..1067)
                     /locus_tag="PLT69_13555"
     CDS             complement(912..1067)
                     /locus_tag="PLT69_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOJ15511.1"
                     /translation="MDVDKNPGTAQRFGIRSIPNLLVFKNGAKVGDIVGAMPESKLIE
                     RIRDILG"
BASE COUNT          184 a          318 c          393 g          182 t
ORIGIN      
        1 caagttctta gggggggcta ccgtggccga gctcacgccg ggcctgaggg aggacatgcg
       61 cattgacccc aaggtccagg gggtcgtggt gacgggggtg gcgtcgaact cggaggccgc
      121 cggcacgggg ctgcgccctg gagatgtggt cctctcgatc aacgggaagc ccaccaggac
      181 gatcgacgac ttcaagaggg tcgttgccgg gctcaagggc atgaagatga gcataagctt
      241 gtacaggcag ggcgcggtca tgaacctgac catcatccgc taggtcgtcc ggggggcgtg
      301 tgaggacggt ggtccagagg gtgagcaggg ccagtgtgaa gacctccggc gtgcaggtct
      361 gctccatcga caaggggctg tgctgcctgg tgggagtgga gaaaggcgac ggcgaggggg
      421 acctcgacta cacggcgcga aagatcgtgg gcctcaggat cttcgaggac gaggagggcg
      481 ccatgaacct cgacgtgcaa accacaggcg gcgacatcct cctcgtgtcc cagttcaccc
      541 tgctggcgga cgcccgcaag gggaggcggc cgtcttttgc ggacgcggag gacggcgcac
      601 aggccaggga gctcttcgat gccctcgtgg ccaaggttcg cgggatcttt cccggcaggg
      661 tggagaccgg cgtgttcggg gccatgatgg aagtggacat cgtgaacaac gggccggtga
      721 ccatcctgct cgacagcagg agacggttct gagccctgac cctcaacgcc ggcgggtttc
      781 ttccctcgtc catgcgagcg gcgatgcggg ccttctttcc cggccgggcg ccgtcgctca
      841 cccccggcgt caaccggccg tcgaggccgc gaggtctcgg ggggccgggg tgaaaagcac
      901 cgccaccccg ctcagccgag gatgtctcgt atgcgctcga tgagtttcga ctcgggcatg
      961 gcgcccacga tgtctcccac cttggcgccg ttcttgaaga cgaggagatt cggtatcgac
     1021 ctgatgccga acctctgggc ggtgcctggg ttcttgtcca cgtccagctt ggcgaag
//
LOCUS       DAOSCR010000777         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_92799_length_1077_cov_2.705479, whole genome shotgun sequence.
ACCESSION   DAOSCR010000777 DAOSCR010000000
VERSION     DAOSCR010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92799_length_1077_cov_2.705479"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="PLT69_13560"
     CDS             <1..>1077
                     /locus_tag="PLT69_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15512.1"
                     /translation="VLIIPVVTLGYFLFSTNITPQALPAMGVHLVIILALSIAAVLVT
                     VHESNAGIASGLGMTVAALQALERGSRDVLPIPLLTKGEIGVIGQYVNVLARSLKSSE
                     EMLEKAFRSSPVGIAIWRLNGGVVLNVNESFTDITGYRLEDVAGRSVDELGLFTSPSE
                     YERAVETLVQGEKVKNREMELSTKWGVTRTAAVSAEIITLAGESCIIAAMEDITEKRT
                     LEREILLVGERERQKIGQDLHDDLSPHLIGIEVMSGLLKKRLEARQVPTIDEVEKIRA
                     LIEQAISKTRRLSRGLCPVFLADHGLESLLQEMSRTIAEVHGIECSFSYERPIVLEDI
                     TACTHIYYIVHEAVHNAVKHGKAD"
BASE COUNT          209 a          345 c          356 g          167 t
ORIGIN      
        1 cggtcctcat cataccggtg gtcacgctcg gctacttcct gttctcgacg aacataaccc
       61 cgcaggcgct gccggccatg ggcgtgcacc tggtcatcat cctcgccctg tcgatcgcgg
      121 cggtcctcgt gacggtccac gagtcgaacg cgggcatagc atcgggcctc gggatgaccg
      181 tggccgccct gcaggccctg gaacgcggct cgagggacgt cctgccgatc ccgcttctca
      241 ccaagggcga gatcggcgtc atcggccagt acgtgaacgt gctcgccagg tcgctcaaga
      301 gctccgagga gatgctcgag aaggccttca ggtcgagccc ggtgggcata gcgatctggc
      361 ggctcaacgg cggggtcgtc ctgaacgtca acgagagctt cacggacatc acggggtatc
      421 gcctcgagga cgtcgccggg aggtccgtgg acgagctcgg gctgttcacg tccccctcgg
      481 agtacgagcg tgcggtcgag acgctcgtcc agggagagaa ggtgaagaac cgcgagatgg
      541 agctctcgac gaagtggggc gtcacccgca ccgcggccgt gtcggcggag atcatcacgc
      601 tggccggcga atcctgcatc atagccgcca tggaggacat cacggagaag aggaccctgg
      661 aacgcgagat cctcctcgtg ggcgagcggg agcgccagaa gatagggcag gacctccacg
      721 acgacctcag tccccacctc atagggatcg aggtcatgag cgggctcctc aagaagaggc
      781 tcgaggcgcg ccaggtgccc accatcgacg aggtggagaa gatcagggcg ctcatcgaac
      841 aggcgatctc gaagacgagg aggctctcca gggggctctg ccccgtgttc ctcgcggatc
      901 acgggctcga gtcgctcctg caggagatgt cccgcaccat cgccgaggtc cacggcatcg
      961 agtgcagctt ctcctacgaa cgccccatcg tgctggaaga catcaccgcc tgcacccaca
     1021 tctattacat cgtgcacgag gcggtgcaca acgcggtgaa gcacggcaag gcggaca
//
LOCUS       DAOSCR010000778         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_93282_length_1073_cov_3.539293, whole genome shotgun sequence.
ACCESSION   DAOSCR010000778 DAOSCR010000000
VERSION     DAOSCR010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93282_length_1073_cov_3.539293"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="PLT69_13565"
     CDS             <1..>1073
                     /locus_tag="PLT69_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15513.1"
                     /translation="VSFFILIVLPLTITGIIISKGVIKVGEEATQANLRILDDNQKQS
                     IAGRAQNVADAVAQFLSDREKDIRIASILPRDEQSYVTFLKSNTRGVVESSSAGIVKV
                     PLSVYREIAFLDKSGKEALKVTVDGAVPAERLKDMSNPANGDYGNEDYFLKAKALAPG
                     EFYLGPVVGRHVSRQEFEAGKRFEGIMRMAAPVFDSGGFAGVVELALDFRHVMEFTDH
                     IVPTEYGMVFAKVNPDDMNYTFLVGRDGTMLAHPAQYLLGGVGPDGNPVPVLDDKNYN
                     DLVKTGGGVMNVLNMGFLDENLPKIHALASSGKSGSFTYTVDNRRIFIAYAHIPFYGS
                     IYTRPEGFGWVGITVDIDRYHKL"
BASE COUNT          228 a          326 c          335 g          184 t
ORIGIN      
        1 gtgagcttct tcattctcat cgtgcttcct ctcaccatta ccgggatcat catcagcaag
       61 ggggtgatca aggtcggtga agaggccacg caggcaaacc tgagaattct cgacgacaac
      121 cagaaacagt cgatagcagg cagggcacag aatgtggcgg atgccgtcgc gcagttcctg
      181 tcggaccgcg agaaggacat ccgcatcgcg tcgatactgc ccagggacga acagtcctac
      241 gtcaccttcc tgaaatcgaa caccaggggc gtggtggagt cctcgagcgc gggcatcgtc
      301 aaggtccccc tttccgtgta ccgcgagatc gccttcctgg acaagtccgg caaagaggcc
      361 ctcaaggtca ccgttgacgg tgcggtgccg gcggagaggc tcaaggacat gtccaacccg
      421 gcaaacggcg actacggcaa cgaggactat ttcctcaagg ccaaggcgct cgcgcccggc
      481 gagttctacc tcggccccgt ggtcggcagg cacgtgagca ggcaggagtt cgaggcggga
      541 aagcggttcg agggcatcat gaggatggcg gcgcccgtgt tcgactcggg cggcttcgcg
      601 ggggtcgtgg aactcgccct cgacttccgc cacgtgatgg agttcaccga ccacatcgtg
      661 cccacggagt acggtatggt gtttgcgaag gtgaaccccg acgacatgaa ctacaccttc
      721 ctcgtgggac gggacggaac gatgctcgcg cacccggccc aatacctcct cggcggcgtg
      781 ggtccggacg ggaacccagt gcccgtgctc gacgacaaga actacaacga cctcgtcaag
      841 accggcgggg gtgtcatgaa cgtcctgaac atgggcttcc tggacgagaa cctccccaag
      901 atccatgcgc tggcctcatc gggcaagtcg ggatcgttca cctacaccgt ggacaaccgc
      961 aggatcttca tcgcgtacgc ccacataccg ttctacgggt cgatctacac gaggcccgag
     1021 gggttcggat gggtgggcat aacggtggat atcgacaggt accacaagct cag
//
LOCUS       DAOSCR010000779         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_93681_length_1070_cov_3.432512, whole genome shotgun sequence.
ACCESSION   DAOSCR010000779 DAOSCR010000000
VERSION     DAOSCR010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93681_length_1070_cov_3.432512"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLT69_13570"
     CDS             complement(<1..211)
                     /locus_tag="PLT69_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15514.1"
                     /translation="MWSDRDAFPVTPGGDCTVFIQGRDANGRVDSRILEERIQEAVRQ
                     GCTKIEVQAYGQHGIGGRLWFTGGSH"
     gene            complement(201..>1070)
                     /locus_tag="PLT69_13575"
     CDS             complement(201..>1070)
                     /locus_tag="PLT69_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOJ15515.1"
                     /translation="WDEILHALPDMKEDPCAIEIKYGQGAKPGDGGLLMWYKVNSLIG
                     AIRGVPVGVSLPSPPTHQTKYSIEESVAKMIQSMYMAWGFRVPVYPKISGTTTALAVL
                     NNIARNPYAAALAIDGEDGGTGAAYNVSMNHMGHPIASNVRDAYLNLVRIGKQNEIPI
                     VAAGGVGKNGNLAANAAALIMLGASAVQVGKYIMQACGGCVGSETDRCNICNIGLCPK
                     GITSQDPRIYRRLDPEDVAQRLVDVFVSFDVELKKIMAPLGRSTSLPIGMSDALGIDD
                     EGAAKRLGIQYVV"
BASE COUNT          155 a          363 c          326 g          226 t
ORIGIN      
        1 cgtgcgaccc cccggtgaac cacagccttc ctccgatccc gtgctgcccg taggcctgca
       61 cctcgatctt cgtgcacccc tgcctgacgg cctcctgtat gcgttcctcg aggatgcggg
      121 agtcgaccct gccgttggcg tcccttcctt gaatgaacac ggtgcagtcg ccgcccggtg
      181 tcacgggaaa ggcatctctg tcagaccaca tactgtatcc ccagtctctt ggccgccccc
      241 tcgtcgtcga tgcccagggc gtcggacatg ccgatgggca gcgacgtgga ccttcccagg
      301 ggggccatga tcttcttgag ctccacgtcg aagctcacga acacgtccac cagcctctgc
      361 gccacgtcct ccgggtcgag cctcctgtat atcctcgggt cctgagaggt tatccccttc
      421 gggcagaggc ctatgttgca gatgttgcac ctgtcggtct cggacccgac acagccgccg
      481 caggcctgca tgatgtactt ccccacctgg acggcgctcg cgccgagcat gatgagcgcc
      541 gccgcgttgg ctgcgaggtt gccgttcttc ccgacaccgc ctgcggccac gatcggtatc
      601 tcgttctgct tgccgatcct gaccaggttg aggtaggcgt ccctgacgtt gctcgcgatc
      661 gggtggccca tgtggttcat ggacacgttg taggcggcgc ccgtaccgcc gtcttcgccg
      721 tcgatggcca acgcggccgc gtacgggttg cgcgcgatgt tgttcaggac ggcgagcgcc
      781 gtggtcgtac ccgagatctt ggggtatacg ggcaccctga atccccaggc catgtacatg
      841 gactggatca tcttggccac cgattcctcg atggagtact tcgtctggtg cgtgggcggc
      901 gaggggaggc tcacaccgac aggcacccct cgtatggcgc cgatgaggct gttcaccttg
      961 taccacatga gcaggccgcc gtccccgggc ttggccccct ggccgtactt gatctctatg
     1021 gcgcaggggt cttccttcat gtcgggcaag gcgtggagga tctcgtccca
//
LOCUS       DAOSCR010000780         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_93811_length_1069_cov_2.910256, whole genome shotgun sequence.
ACCESSION   DAOSCR010000780 DAOSCR010000000
VERSION     DAOSCR010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93811_length_1069_cov_2.910256"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="PLT69_13580"
     CDS             complement(<1..>1069)
                     /locus_tag="PLT69_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ15516.1"
                     /translation="TRIINAVERGAKLLLFDPREIQLAPFASLHLRQNFGTDVALVNG
                     MMNIIIQEDLYDRPYVESRTEGFDELKALVSRYTPKVVEQITGVRSADLYRAAVMYAQ
                     ADKAMIVYAMGITQHVTGTDNVKTLANLAMLTGHVGRHSTGVNPLRGQNNVQGACDMG
                     GLPGVFTGYQRVNDPASVQKFEKAWETTGLNTKPGLTITGMVEAAHSGSLKALYVMGE
                     NPLLSDPDLTHAEHALGNLDFLVVQDIFLTETAALADVILPAACFAEKDGTYTNTERR
                     VQLSRKAVEPPGQARADWEIVSELLSRLGCCGTYPDTESIMREINALTPSYAGITYRR
                     LASGYGLQWPCPNEEHPGTVFL"
BASE COUNT          155 a          328 c          371 g          215 t
ORIGIN      
        1 gcaggaatac ggtgcccggg tgttcctcgt tggggcaggg ccactggagc ccgtaccccg
       61 aggcaagcct ccggtacgtg atgcccgcgt acgagggggt cagggcgttg atctcgcgca
      121 tgatcgactc ggtgtccggg taggtcccac agcagccgag cctcgagagg agctcggaca
      181 cgatctccca gtcggccctc gcctggccgg gcggctccac cgccttcctg gaaagctgga
      241 cgcggcgctc ggtgttcgtg tacgttccgt ctttctctgc gaagcaggcc gccggcagga
      301 tgacgtccgc cagggccgcc gtctcggtga ggaagatgtc ctgcaccacg aggaagtcga
      361 ggttgccgag ggcgtgttcc gcgtgggtga ggtcggggtc gctcaagagg gggttttcgc
      421 ccatgacgta gagggccttg agggacccgg agtgtgccgc ctcgaccatg ccggtgatgg
      481 tgagcccggg cttggtgttc agcccggtgg tctcccatgc cttctcgaac ttctggaccg
      541 atgccgggtc gttcacccgc tggtagcccg tgaacacccc tggcagcccc cccatgtcgc
      601 acgccccctg gacgttgttc tgccccctga gcgggttcac accggtcgaa tgcctcccga
      661 cgtggccggt gagcatcgcg aggttggcga gggtcttgac gttgtcggtg ccggtgacgt
      721 gttgcgtgat gcccatggcg tagacgatca tggccttgtc tgcttgcgcg tacatgaccg
      781 cggcacggta gaggtccgcg gacctcaccc ccgttatctg ttccacgacc ttgggcgtat
      841 accgcgagac gagcgccttc agctcgtcga acccctcggt gcgggactcc acgtatggcc
      901 ggtcgtagag gtcttcctgg atgatgatgt tcatcatgcc gtttacgagc gcgacgtcgg
      961 tgccgaagtt ctgcctgagg tggagcgatg cgaagggcgc cagctgtatc tcgcggggat
     1021 cgaagaggag gagctttgcg ccccgttcca ccgcgttgat gatacgggt
//
LOCUS       DAOSCR010000781         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_94079_length_1067_cov_2.719368, whole genome shotgun sequence.
ACCESSION   DAOSCR010000781 DAOSCR010000000
VERSION     DAOSCR010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94079_length_1067_cov_2.719368"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1067
                     /locus_tag="PLT69_13585"
     CDS             <1..>1067
                     /locus_tag="PLT69_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ15517.1"
                     /translation="APLKRKRLLRTHVLGAMVRTGACALIWVLCLAALFKGAVGPGSF
                     VGASASLLFVVLMNVPMLLVLRFTSSKGWFEFFSFCVNVLETFGYTAFIYFVGGFRST
                     YLTPIYAAMIFYVGVMAPMRYPLVLAGVSSGMFSVMVYLEHFGYLPHQNVFIRYAYTW
                     DMIVFVLFILTAVLFLIACMAAYTSVVLNEAKKNLKKKNLALEKVNAMLSEEIHERIK
                     AEALLRASEEKLKDIFDNVPDALFTHDLQGRFSDMNPCFREMIGLDQGAPLPGDLSIR
                     DLVPGRFWPAVDAYLEDIVRKGRCEGVMSIVGMDGKKRILEFKNTLMKDGCGRPVGVR
                     GCARDITERTRAEKERAKLQE"
BASE COUNT          206 a          323 c          328 g          210 t
ORIGIN      
        1 ggcgcccctg aagaggaaga gactcctcag gacgcacgtc ctcggggcga tggtgaggac
       61 cggcgcgtgc gccctcatat gggtcctctg cctggccgcc ctcttcaagg gcgccgtcgg
      121 cccggggtcg ttcgtcggtg cgagcgcatc gctgctcttc gtcgtcctca tgaacgtgcc
      181 gatgctcctt gtcctcaggt tcacctcgtc gaagggatgg ttcgagttct tttccttctg
      241 cgtcaacgtg ctcgagacgt tcgggtacac ggcgttcatc tacttcgtcg gcgggttcag
      301 gtccacgtac ctgaccccga tctacgccgc catgatcttc tacgtggggg tcatggcgcc
      361 catgaggtat ccgctcgtcc tcgcgggcgt aagctccggc atgttctcgg tcatggtcta
      421 cctggagcac ttcggttacc ttccccacca gaacgtgttc atccgctatg cgtacacctg
      481 ggacatgatc gtgttcgtgc tcttcatcct gacggcggtc ctcttcctca tcgcctgcat
      541 ggccgcctac acctcggtgg tcttgaacga ggcgaagaaa aacctcaaga agaagaacct
      601 cgccctcgag aaggtcaacg ccatgctctc ggaagagata cacgagagga tcaaggccga
      661 ggcattgctc agggcgagcg aggagaagct caaggacata ttcgacaacg tcccggacgc
      721 cctgttcacc cacgaccttc aaggacgctt ctccgacatg aacccttgtt tcagggagat
      781 gatcggcctc gatcaaggcg cccccctccc cggtgacctg agcatacggg atcttgtccc
      841 ggggaggttc tggcccgccg tcgacgcata cctcgaggac atcgtcagga agggcaggtg
      901 cgagggggtg atgagcatcg tgggcatgga cgggaaaaaa cgtatcctgg agttcaagaa
      961 cacgctcatg aaagacggct gcggaaggcc cgtgggcgtg agggggtgcg ccagggacat
     1021 caccgagagg acgagggcag aaaaggagcg cgccaagctc caggaac
//
LOCUS       DAOSCR010000782         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_94102_length_1067_cov_2.131423, whole genome shotgun sequence.
ACCESSION   DAOSCR010000782 DAOSCR010000000
VERSION     DAOSCR010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94102_length_1067_cov_2.131423"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLT69_13590"
     CDS             complement(<1..469)
                     /locus_tag="PLT69_13590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ15518.1"
                     /translation="MKVRLVHIQGPLAGQIQEFTEPSISIGRHPSCQVTFPADLKTVS
                     RTHALIVREGNRCKLIDKSTNGTFVNGRRVQGEVFLKDGDVIMFTDGGPKVSFLAELV
                     DAAPPRPPTPPESTDAAFGRQEAAAQAQGTPARGAQAPAPRQGPPPSVPQAPAG"
     gene            574..>1067
                     /locus_tag="PLT69_13595"
     CDS             574..>1067
                     /locus_tag="PLT69_13595"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOJ15519.1"
                     /translation="MRIEARGLTHVGMRRSSNEDAFLVREDLGMCTVADGMGGAAAGE
                     VASAFFMDAALEVFGRADPLGVAEDLVKNAFFLANGRILDHAGSHPECSGMGCTAEIL
                     AVRGDTVVIGHVGDSRTYRFRDGELRQLTVDHSLVQSQVDSKAITAEQARVHPMRHVI
                     LRAVG"
BASE COUNT          159 a          328 c          407 g          173 t
ORIGIN      
        1 cacctgcagg cgcctggggc acggagggtg gcggcccctg ccggggggcg ggggcctgcg
       61 cgccgcgcgc gggcgtgcct tgggcttggg ccgcggcctc ctggcgtcca aaagccgcgt
      121 ccgtgctctc gggcggcgtc ggtgggcggg ggggcgcggc gtccacgagt tccgcgagga
      181 agctcacctt gggcccgccg tcggtgaaca tgatcacgtc gccgtccttg aggaagacct
      241 cgccttggac cctcctgccg ttgacgaacg tcccgttggt gctcttgtcg atgagcttgc
      301 agcggtttcc ctcgcgcacg atgagggcgt gcgtccggga gacggtcttg agatcggccg
      361 ggaacgtcac ctggcaggaa gggtgtctcc cgatgctgat ggatggctcg gtaaactcct
      421 ggatctggcc tgcaagcggg ccctggatgt ggacgagccg caccttcatg accgggatct
      481 ccctcatacc gaaggtgaat accggctcgc acagggcatg tcaaggggaa gggcccgcgg
      541 cggggacggg ttgaagaggg cggagggggc cctgtgagga tcgaggctcg aggccttacg
      601 cacgtgggga tgaggcgttc gtccaacgag gacgccttcc tcgtccgcga ggacctcggc
      661 atgtgcacgg tggcggacgg catggggggg gcggccgccg gggaggtcgc gagcgccttc
      721 ttcatggatg ccgcactcga ggtgttcggc cgggccgacc ccctcggcgt cgcggaagac
      781 ctggtgaaga acgccttctt cctcgccaac ggccggatcc tcgaccatgc ggggagtcac
      841 cccgaatgct cgggcatggg ctgtaccgcc gagatcctcg ccgttcgcgg ggacacggtg
      901 gtcatcggcc acgtgggaga cagccggacg tacaggttca gggatgggga gctccgtcag
      961 ctcaccgtgg accactcgct cgtccagtca caggtggaca gcaaggcgat aacggcggag
     1021 caagccaggg tgcatcccat gcgccacgtg atcctcaggg cggtggg
//
LOCUS       DAOSCR010000783         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_94486_length_1064_cov_2.642220, whole genome shotgun sequence.
ACCESSION   DAOSCR010000783 DAOSCR010000000
VERSION     DAOSCR010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94486_length_1064_cov_2.642220"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLT69_13600"
     CDS             <1..769
                     /locus_tag="PLT69_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOJ15520.1"
                     /translation="EGVDITVEPVKDYRFALILDGEGLEDGVTETDPQKTGLRPLEVR
                     PLTERSRKTASLLNAWIGKAFEVLKNESPANGCTLRGIAKDPGLPSYREVYGLNACAI
                     ATYPMYKGLARLVGMDVLEAGDTIASEIETLKSAWDRYDFFFFHVKKTDSAGEDGDFD
                     AKVRVIEETDAVLPELLALKPSVLIITGDHSTPASMKSHSWHELPVLFVADNIRPDGV
                     TEFGERACMAGGLGHLRHTDLMPLAMAHADRLTKYGA"
     gene            771..>1064
                     /locus_tag="PLT69_13605"
     CDS             771..>1064
                     /locus_tag="PLT69_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HOJ15521.1"
                     /translation="MAASRPRVAILFGGVSTEHDVSVVSGASIARSIDTDRFDPVYVG
                     ITKEGRWLLGDGAFDALRGGGTEGVEPVILSPDPQHKGFVHIESGRLTGVDVVF"
BASE COUNT          200 a          329 c          358 g          177 t
ORIGIN      
        1 ggagggggtt gacataacgg tggagccggt caaggactac cggttcgccc tcatcctcga
       61 cggtgaaggt ctcgaagacg gcgtgaccga gaccgatccc cagaagacgg gcctcaggcc
      121 gttggaggta aggccgctca ccgagcgttc gcgcaagacc gcctctctcc tcaacgcgtg
      181 gatcggcaag gcgttcgagg tcctcaagaa cgagtccccg gcgaacgggt gcactctcag
      241 gggcatcgcc aaggaccccg ggctgccatc ctaccgcgag gtctacgggt tgaacgcgtg
      301 cgcgatagcc acctatccca tgtacaaggg tctcgcacgg ctcgtgggca tggatgtgct
      361 ggaggccggc gacaccatag cgagcgagat cgagacgctg aagtccgcct gggacaggta
      421 cgacttcttc ttcttccatg tcaagaagac ggactcggca ggcgaggacg gagacttcga
      481 cgccaaggtg agggtcatcg aggagacgga cgcggtcctc cccgagctcc tagcgctcaa
      541 accgagcgtg ctcatcataa cgggcgatca ctcgacgccg gcttccatga agtcgcactc
      601 gtggcacgag ctgccagtgc tcttcgtcgc ggacaacata cgaccggacg gcgtcaccga
      661 gttcggggaa cgcgcctgca tggcgggagg cctcgggcac cttcggcaca ctgacctcat
      721 gcccctggcc atggcgcatg cggacaggct gacaaagtac ggtgcttgac atggccgcgt
      781 cgagaccgcg cgtagccatc ctcttcgggg gtgtctccac ggagcatgac gtctccgtgg
      841 tctcgggggc ttccatcgcc cggtccatcg atacggacag gttcgatccc gtgtacgtgg
      901 ggatcaccaa ggaaggcagg tggctcttgg gcgacggggc cttcgatgcg ctgagggggg
      961 gcgggaccga aggcgtggaa cccgtgatcc tctcgcccga cccgcagcac aaggggttcg
     1021 tgcacatcga gagcggcagg ctcaccggtg tggacgtcgt cttc
//
LOCUS       DAOSCR010000784         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_94882_length_1061_cov_3.238569, whole genome shotgun sequence.
ACCESSION   DAOSCR010000784 DAOSCR010000000
VERSION     DAOSCR010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94882_length_1061_cov_3.238569"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLT69_13610"
     CDS             <1..740
                     /locus_tag="PLT69_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ15522.1"
                     /translation="FDIQKVLERLLLKGSIVVKKAYCDWERYRDYKPVMHEASFELIE
                     IPHLRQSGKNSADIRLVVDALDLCYTKSHVDTFVIISGDSDFSPLVSKLRENNKIVIG
                     VGVKNSSSELLIANCDEFIFYDDLVRESEAKRKKTRKQASRQAAAQTKAEHRENGEED
                     KRQKAIDLVVETVEALFDERGEEDKLWGSMVKQALRRRMPGFNESYYGFRSFSRLLEE
                     ARDRGLLELELDEKSGSYIIKNVIHDE"
     gene            complement(820..>1061)
                     /locus_tag="PLT69_13615"
     CDS             complement(820..>1061)
                     /locus_tag="PLT69_13615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ15523.1"
                     /translation="RGFLQKGLMDRYGRWAGFVLTTAVYSLVHLWSFNFMLVCAALVA
                     GAFWGFLYMRLGRLDVVIVSHALWSSVIFAVFPVA"
BASE COUNT          245 a          318 c          349 g          149 t
ORIGIN      
        1 agttcgacat ccagaaggtg ctcgagcggc tgctgctcaa ggggtcgatc gtcgtcaaga
       61 aggcgtactg cgactgggaa aggtaccgcg actacaagcc ggtcatgcac gaggcgtcct
      121 tcgagctcat cgagatcccg cacctcaggc agtcgggcaa gaactcggca gacatcaggc
      181 tcgtcgtgga cgcgctggac ctctgctaca cgaagtccca cgtggacacc ttcgtcatca
      241 tcagcgggga ttcggacttc tcccccctgg tgagcaagct gagggagaac aacaagatcg
      301 tcatcggcgt cggcgtgaag aactcgtcat ccgagctcct catcgcgaac tgcgacgagt
      361 tcatcttcta cgatgacctc gtccgcgaga gcgaggccaa gaggaagaag accaggaagc
      421 aggcatccag gcaggctgcg gcgcagacaa aggccgaaca cagagagaac ggcgaggaag
      481 acaagaggca gaaggcgatc gacctcgtgg tcgagaccgt ggaagccctg ttcgacgagc
      541 gcggcgaaga ggacaagctc tgggggtcca tggtcaagca ggccctcagg cgccgcatgc
      601 ccggtttcaa cgagagctac tacgggttca ggtccttcag caggctgctg gaggaggcga
      661 gggacagggg gctgctcgag ctcgaactgg acgagaagtc ggggagttac atcatcaaga
      721 acgtgatcca cgacgagtga ggcaccaggc ccgcgcaggc cgcgacaggc ggccttcggc
      781 tcgtcgccgc agggcggccg catgggcgtc tttcccgctt caggccacgg gaaagaccgc
      841 gaagatcacc gagctccaga gggcgtggga gacgatcacc acatcgagcc tgccgagcct
      901 catgtagagg aaaccccaga aggcgccggc cacgagggcc gcgcagacga gcatgaagtt
      961 gaacgaccag aggtggacca gggagtagac ggccgtcgtg agcacgaacc cggcccatct
     1021 cccgtacctg tccatgagcc ccttctgcag gaacccccgc c
//
LOCUS       DAOSCR010000785         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_95509_length_1056_cov_3.168831, whole genome shotgun sequence.
ACCESSION   DAOSCR010000785 DAOSCR010000000
VERSION     DAOSCR010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95509_length_1056_cov_3.168831"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1056)
                     /locus_tag="PLT69_13620"
     CDS             complement(<1..>1056)
                     /locus_tag="PLT69_13620"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOJ15524.1"
                     /translation="ALRPVLGAAVSLFDLKAALDEAVHYRERARRRLLDVYKRGIASQ
                     AGGDISVVLLGRPYTVLCSDLNKGVADIFASQGVRVFYQDMVSQKEAPSSWINAMLQD
                     VPWRYAAGVLEAASAIAHQEGVYPVLVTSFRCTPDTMTLECFKRIMHESAKPYLVIQL
                     DGHDSKAGYETRIEAAVRSFRNHHRLGRGPARVRLPDILPSRSLRRPIVYIPDWDRFS
                     CRLLAANLRREGLDARLLEHSSSSVTEGLRYNTGQCIPIHIIAQEYMDTMRRHDVDPQ
                     RCALWIPRGEIPCNLKLIPHHIKAALQAHGHGMERAEVYHGELSMIDLSVRASFGVYF
                     SFMFGGLASGQTRITGLL"
BASE COUNT          175 a          340 c          349 g          192 t
ORIGIN      
        1 agcaggcccg taatgcgcgt ctgcccgctc gcaaggccgc cgaacatgaa ggagaagtac
       61 accccgaagg acgcacgcac cgagaggtcg atcatggaga gttccccgtg atacacctct
      121 gcacgctcca tgccgtggcc gtgggcctgc agcgccgcct ttatgtggtg gggaatgagt
      181 ttgaggttgc acgggatctc ccccctgggg atccacaggg cgcagcgctg ggggtccacg
      241 tcgtggcgtc tcatggtgtc catgtattcc tgggcgatga tgtggatggg gatgcactgc
      301 cccgtgttgt acctgaggcc ctcggtcacg ctcgagctcg agtgttcgag gagtcgtgcg
      361 tccaggcctt ccctcctgag gttggccgcg agcagcctgc acgagaacct gtcccagtcg
      421 gggatgtaca cgatgggccg cctgagcgat ctcgacggca ggatgtccgg cagcctcacc
      481 cgggccggtc ccctgcccag gcggtggtgg ttcctgaacg acctcacggc cgcttcgatc
      541 ctcgtctcgt accccgcctt cgagtcgtgg ccgtcgagct ggatcaccag gtagggcttg
      601 gcgctctcgt gcatgatcct cttgaagcac tcgagggtca tggtgtcggg tgtgcaccga
      661 aacgaggtga cgaggaccgg gtacaccccc tcctggtgcg cgatggcaga ggcggcctcg
      721 agcacccccg cggcatagcg ccaaggcacg tcctggagca tggcattgat ccaggacgag
      781 ggcgcctctt tctgtgagac catgtcctgg tagaacaccc tgactccctg cgacgcgaag
      841 atgtccgcga cgcccttgtt gagatcgctg cacaagaccg tgtacggccg gcccaggagc
      901 acgacggaga tatccccgcc ggcttgcgat gcgatacccc tcttgtacac gtccaggaga
      961 cgtctcctgg cgcgctcccg gtagtgaacg gcctcatcga gcgccgcctt gaggtcgaag
     1021 aggctcacgg cggcgccgag caccggcctg agggcc
//
LOCUS       DAOSCR010000786         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_95645_length_1055_cov_3.340000, whole genome shotgun sequence.
ACCESSION   DAOSCR010000786 DAOSCR010000000
VERSION     DAOSCR010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95645_length_1055_cov_3.340000"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1055
                     /locus_tag="PLT69_13625"
     CDS             <1..>1055
                     /locus_tag="PLT69_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ15525.1"
                     /translation="AIGLLAGGVAHDLNNILSGLVSYPELLLMELPEGSPLEKPLLTI
                     KQSGEKAASIVQDLLTMARRGVRVSERLNINNVVRDYLASLDHERLAKEHPGVRLEVE
                     LAPDVVDIEGSPIHIFKTVMNLVSNAFESIEKEGVVRISTFNRTLRRPLVGHDKIGEG
                     TYAVLVVADTGVGMTEQDKQHIFEPFYTRKVMGRSGTGLGMAVVWGAVKDHNGHLELE
                     SEPGKGTVFTLSFPECPVSREGEGSTVSHGCRHDDVEGEGSAAWEVSSEGPRPRILVV
                     DDMEEQRELARMILQRLGYEVETASSGEEALDLVKDSPPDLVLLDMIMGDGMDGLETY
                     RRIREVRPGQKVIIMSG"
BASE COUNT          210 a          313 c          366 g          166 t
ORIGIN      
        1 gccatcggcc ttctggcggg cggcgtggcc catgacctca acaacatcct ctcgggcctg
       61 gtgagctatc ccgagctcct cctcatggag ctccccgagg ggagcccgct cgaaaagccc
      121 ctgctcacca tcaagcagtc cggcgagaag gcggcatcca tcgtgcagga tctcctcacc
      181 atggcgaggc gcggggtgag ggtctccgag cgtctgaaca tcaacaacgt cgtcagggac
      241 tacctggcaa gccttgacca cgagaggctc gcgaaggagc atccaggggt gaggctggag
      301 gtggagttgg ccccggacgt ggtggacatc gagggctcgc ctatccatat cttcaagacc
      361 gtcatgaacc tcgtctccaa tgccttcgag tcgatcgaaa aagagggtgt cgttcgcatc
      421 tccaccttca acaggacgct tcgcaggccg ctcgtgggcc acgacaagat cggggaggga
      481 acgtacgcgg tgctcgtcgt tgcggacacc ggggtgggca tgaccgagca ggacaagcag
      541 cacatcttcg agccgttcta cacgaggaag gtcatgggaa ggagcgggac cggcctcggc
      601 atggccgtgg tgtggggggc ggtcaaggat cacaacggac acctggagct cgagagcgag
      661 cccggcaagg ggacggtctt caccctctcc ttccccgagt gcccggtttc ccgtgagggg
      721 gaaggctcca ccgtcagcca cgggtgccgg cacgatgacg tcgagggcga ggggagcgcg
      781 gcatgggaag tgtcctcgga agggccgcgg ccgaggatcc tcgtggtcga cgacatggag
      841 gagcagcggg agctcgcccg catgatcctt caacgactgg gatacgaagt cgagaccgcc
      901 tccagcgggg aagaggcgct cgacctcgtg aaggactcac ccccggacct cgtcctgttg
      961 gacatgatca tgggcgacgg catggacggg ctcgagacct accggaggat acgcgaggtg
     1021 agaccgggcc agaaggtgat catcatgagc gggtt
//
LOCUS       DAOSCR010000787         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_95794_length_1054_cov_2.718719, whole genome shotgun sequence.
ACCESSION   DAOSCR010000787 DAOSCR010000000
VERSION     DAOSCR010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95794_length_1054_cov_2.718719"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1054
                     /locus_tag="PLT69_13630"
     CDS             <1..>1054
                     /locus_tag="PLT69_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOJ15526.1"
                     /translation="REIASRELGNEYSRIFLKLLSEGFAILRSAGMSTFEDPQEALDR
                     SRAIRRDAVERMPELLEEFARAAGENGAVVLWARDARHACDQIVGLARERGVRFVAKG
                     KSMITEEIGLNRALEESGVEVFETDLGEFVTQTMGLPPFHLVGPAINIPPDKIGEAFF
                     AKGVIREPDSDPVRLGKAVRAHLREKFRALDMGVVGVNMAIASTGTLVNVENEGNIRM
                     IKSSPRTLVAVMSIEKVVPTLEDALHLLRVLCRNCTGQKMSGYVTFDSGPAGRGEKDG
                     PEELFVVIVDNKRSAIYQDPLARQVLRCIRCGACLNTCPVYARIGGYPYGGVYSGPMG
                     AVLSPLLHGIERTSDLF"
BASE COUNT          188 a          333 c          364 g          169 t
ORIGIN      
        1 cagggagatc gcctcgaggg agcttggaaa tgaatacagc cggatcttcc tgaagctcct
       61 ctcggagggg ttcgcgatcc tgcgctcggc cggcatgtcc accttcgagg acccccagga
      121 ggccctcgac cgttcccgcg cgatacgtcg cgacgccgtc gagcgcatgc cagaactcct
      181 ggaggagttc gcccgcgccg caggggaaaa cggggccgtg gtcctgtggg ctcgtgacgc
      241 tcggcacgcg tgcgaccaga tcgtcgggct cgcccgcgaa cgcggggtga ggttcgtggc
      301 caagggcaag tccatgatca ccgaggagat cggcctgaac agggcgctgg aagagagcgg
      361 cgtggaagtg ttcgagacgg acctcggaga gttcgtcacc cagaccatgg ggctcccccc
      421 ctttcacctc gtggggcccg cgatcaacat acccccggac aagatcggcg aggcgttctt
      481 cgccaagggt gtcattcgcg agcccgactc cgaccctgtg cggctcggca aggcggtcag
      541 ggcccacctc agggagaagt tcagggcgtt ggacatgggc gtcgtgggcg tcaacatggc
      601 gatagcctcc accggcaccc tggtcaacgt ggagaacgag gggaacatcc gcatgatcaa
      661 gtcctccccg aggaccctcg tggccgtgat gagcatcgag aaggtggtgc ccacgctcga
      721 agacgcgctg cacctcctca gggtcctgtg caggaactgc accgggcaga agatgtccgg
      781 ttacgtgacg ttcgattcgg gaccggcggg gaggggggag aaggacggcc cggaggaact
      841 cttcgtggtg atcgtggaca acaagcgctc cgcgatctac caagaccccc tcgcccggca
      901 ggtgctcagg tgcatcaggt gcggcgcctg cctgaacacc tgccccgtgt acgcgaggat
      961 cggcggctac ccctacgggg gggtgtactc gggcccgatg ggggccgtcc tgtcgccgct
     1021 cttgcacggg atagagcgca cttccgacct cttc
//
LOCUS       DAOSCR010000788         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_96886_length_1046_cov_2.406660, whole genome shotgun sequence.
ACCESSION   DAOSCR010000788 DAOSCR010000000
VERSION     DAOSCR010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96886_length_1046_cov_2.406660"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..>1046
                     /locus_tag="PLT69_13635"
     CDS             459..>1046
                     /locus_tag="PLT69_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15527.1"
                     /translation="MAGEGQVRGPDEGGTGGDSGGQGPTGTAEVGEETEPPSQDAPGE
                     ESGCSTSGYMEVRGVSALRKESPDEDPSWELGLQVEGKTAPWNAYKVSLVSVQDGKVI
                     EPSHGGAFHAFNRVYQDMTPYIDIDEAYLDVYTGRVDIRLGIQKFAWGRLDEINPTDN
                     LNPEDLTEGGTNDEVDRKIGVPALKVNAYSDAANVE"
BASE COUNT          212 a          329 c          387 g          118 t
ORIGIN      
        1 gcaccctcga tagccttgca tgcccggccg acgatgtgcc gttcgccgcg ccgacgacgg
       61 atgtgcagcc cgtacccctc gacagtgtcg catgggcatc cgcgagtcca gcgcaaacgg
      121 cgcctctcgt actcgcacaa caggacacgc cccgagcgat ggggcaggcg ccgtccccac
      181 gcggtgcgga tacagccgca gggccgcaag agggtgcggg ggccgctgag ggcggtcaag
      241 gggatgcctc accgggcgaa ccccctgcgc aagggccgac ggctggagag gccggggggg
      301 atggaggccg ggaaaggtcc gccgggctcg aggaaggcgg ccgggcaggg gatggaacct
      361 ccgggcaacc ggtgaacgcg cctcctccgg gcgtcgcgga tgcagcctcc acgcaggaca
      421 gtcccacggg agacgcccag gcccaagagg aagggcccat ggctggtgaa gggcaggtcc
      481 gagggcccga cgagggaggt acgggagggg attcaggcgg gcaaggtcca acggggacag
      541 ccgaggtcgg ggaggagacc gaacccccct cgcaggacgc cccgggggaa gagagcgggt
      601 gctcgacaag cgggtacatg gaggtcaggg gcgtctctgc attgagaaag gaaagtcccg
      661 acgaggaccc atcctgggag ctcggtctcc aggtcgaggg gaagacagcg ccgtggaacg
      721 cgtacaaggt ctcgctcgtg tcggtccagg acggcaaggt catcgagccg agccacggcg
      781 gcgccttcca tgccttcaac cgcgtctacc aggacatgac cccctacatc gacatagacg
      841 aggcatacct cgacgtctac acgggccggg tggacatcag gctcgggatc cagaagttcg
      901 cctgggggag gctcgacgag atcaatccca ccgacaacct caaccccgag gatctcacgg
      961 aaggcgggac gaacgacgag gtggaccgca agatcggcgt gcccgcgctc aaggtgaacg
     1021 cctactcgga cgcggcgaac gtcgag
//
LOCUS       DAOSCR010000789         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97026_length_1045_cov_2.431313, whole genome shotgun sequence.
ACCESSION   DAOSCR010000789 DAOSCR010000000
VERSION     DAOSCR010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97026_length_1045_cov_2.431313"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1045
                     /locus_tag="PLT69_13640"
     CDS             <1..>1045
                     /locus_tag="PLT69_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOJ15528.1"
                     /translation="GSIDREGTLKANTVFRFDGFNDNSYRAFFSRLSVEERQQYIEKL
                     VMRTAPGGVLTSCEILPRNMHDTSEPIVVRAGFEVKEWAIPGNDAVMLPMIRFGRSVG
                     MANVLIGKMGLKTRRYPYVTEVACGVQESLRIDLGDSVGEVVSLPDYEPVENHGTAWN
                     MNVSVQGRVLAMDNVFKLKVPEYSPEEYLELQETLKIIEADSRKMPIFSPAPSAPLVQ
                     SKAWYTSSRDYDPDALVLDELHEYDVIDDTTWTETRRMKMKVFTYAGVKNYSDLRIHY
                     NPACEKVEVLNAVVTSPEGVQTSIQEHEINIMDADWVADGPRYPGSKIMVVSLPGVDE
                     GSVIDYTIKRTKTG"
BASE COUNT          236 a          301 c          318 g          190 t
ORIGIN      
        1 cgggagcatc gacagagaag gcaccctgaa ggccaacacc gtattccgtt tcgacggatt
       61 caacgataat tcataccgcg cgttcttctc gcggctctcg gtcgaggaac gccagcagta
      121 tatcgagaag cttgtcatga ggaccgcacc cggaggtgtt ctcacctcgt gcgagatcct
      181 gccccgcaac atgcacgaca cgtctgagcc catcgtagtc cgggccgggt tcgaggtcaa
      241 ggaatgggcc atcccgggca atgacgcggt gatgctcccc atgatccgtt tcggcaggag
      301 cgtgggcatg gcgaatgtgc tcatcggcaa gatgggcctg aagacgcgga gataccctta
      361 tgtcaccgag gtggcgtgcg gggtccagga atcgctcagg atcgatctgg gcgactcggt
      421 gggcgaggtg gtgagcctgc ccgactatga accggtggaa aaccacggga ccgcttggaa
      481 catgaacgtg tccgtccagg gcagggtgct ggccatggac aacgtcttca agctcaaggt
      541 gcctgaatat tcgccagagg aatacctcga gctccaggaa accctgaaga tcatcgaggc
      601 ggattcccgc aagatgccca tcttctcgcc cgctccgtcc gccccgctgg ttcagagcaa
      661 ggcctggtac acgtcatccc gggactatga tcccgatgcc ctcgtcttgg acgagctcca
      721 cgagtatgac gtgatcgacg acaccacctg gaccgagacc cggcgcatga agatgaaggt
      781 gttcacctac gccggcgtga agaactacag cgatctgcgc attcactata atcctgcgtg
      841 cgaaaaggtc gaggtgctca acgcggtggt gacatctccc gaaggggtgc agacttcaat
      901 ccaagagcat gagatcaaca tcatggacgc cgactgggtc gcggacggcc cgagatatcc
      961 gggctcgaag atcatggtag tgagccttcc gggcgtggat gaaggaagcg tcatcgacta
     1021 taccatcaag cgcaccaaga ccgga
//
LOCUS       DAOSCR010000790         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97064_length_1045_cov_2.080808, whole genome shotgun sequence.
ACCESSION   DAOSCR010000790 DAOSCR010000000
VERSION     DAOSCR010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97064_length_1045_cov_2.080808"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>1045)
                     /locus_tag="PLT69_13645"
     CDS             complement(106..>1045)
                     /locus_tag="PLT69_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15529.1"
                     /translation="AALEQAPDLMMEVIAQREAIRKTAWDAVRRKQYWAVVGSGVNKV
                     ASDEVRIKLSELCYKTISSDIIEDKKHIDLSSEPLILVCASGSPPMVLDDIVKDVAIF
                     KAHASTVVVIADRGAGESFENVADAVIEVPGADFPLCVILNTLAGHIWGYYAALSLDE
                     QARTFKRFRTELQERAKEHKRLQYTVYESVKDRELHRLVDAFEADFKAWRVKGELASL
                     SNDVASDITLLLKYARGKLPVEDFWMEFEGKRVSSSPLDMLDLTLKRPWKSSRGPWTQ
                     SDTRRRPSPWARAGRWRSPRGPCSRCWSNSGSPRRT"
BASE COUNT          168 a          353 c          317 g          207 t
ORIGIN      
        1 cgggcatgcc cgcctcgtcc aacccttcga cccggtagag ggtgtagccc ttgatcctga
       61 cgatcgcgcc ctggagcctc ttgaggaaga gcacctcccg ggccctcacg ttctccgggg
      121 agaacccgag ttcgaccagc accgagaaca cgggcccctt ggggacctcc accttcctgc
      181 tcgtgcccac ggtgacggtc ttcgcctggt gtcggattgc gtccacgggc ctcgagagct
      241 cttccacggc ctcttgagcg tgaggtcgag catgtcgagc ggggacgagg aaacgcgctt
      301 tccctcgaac tccatccaga agtcctcgac cgggagcttg cccctggcat acttgaggag
      361 gagcgtgatg tccgaggcga cgtcgttgct cagagaagcg agctcgccct tcaccctcca
      421 cgccttgaaa tcggcctcga acgcgtcgac gaggcggtgg agctccctgt ccttgacgct
      481 ctcgtacacg gtgtactgga gcctcttgtg ctccttggcc ctctcctgga gttcggtccg
      541 gaaacgcttg aaggtccttg cctgctcgtc caggctcagc gccgcatagt agccccagat
      601 gtggccggcc agggtgttga ggatgacgca gaggggaaag tctgcccccg gcacctcgat
      661 gacggcgtcg gcgacgttct cgaaactctc ccccgccccc cggtctgcga tcaccaccac
      721 ggtggatgca tgcgccttga agatcgccac gtccttcacg atgtcgtcga ggaccatggg
      781 cgggctcccc gatgcgcaca cgaggatgag gggttcggac gagaggtcga tgtgcttctt
      841 gtcctcaatg atgtccgagg aaatggtctt gtaacagagc tctgagagct ttatccgcac
      901 ctcgtcggag gcgaccttgt tgacgccgct gcccaccacc gcccagtact gcttcctccg
      961 caccgcgtcc caggcggtct tccttatggc ctcccgctgc gcgatgacct ccatcatgag
     1021 gtcgggcgcc tgctcgagcg cggcg
//
LOCUS       DAOSCR010000791         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97137_length_1044_cov_2.974722, whole genome shotgun sequence.
ACCESSION   DAOSCR010000791 DAOSCR010000000
VERSION     DAOSCR010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97137_length_1044_cov_2.974722"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLT69_13650"
     CDS             <1..319
                     /locus_tag="PLT69_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOJ15530.1"
                     /translation="RLMAKISHLPHLLSYASMGLAEDVHIHRQLLGAGFRDFTRIAAS
                     DPLMWKDILMDNRDNVLPLVEAYIEELHRLKTLVEEDRATELETELSRFAQIRRALYE
                     SPR"
     gene            303..>1044
                     /locus_tag="PLT69_13655"
     CDS             303..>1044
                     /locus_tag="PLT69_13655"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ15531.1"
                     /translation="MKAHGEITPPPDKSITHRCFMIGSLAHGTTVIRNALESEDIAST
                     RKALAALGASIRKDGDSYLVDGSTLREPTHVVDAGNSGTTARLVSGIVSGIEGITVMT
                     GDESLIKRPMARVIKPLELMGARFMARSGSFLPMAVRGGKLRGIFHPMEVASAQVKSA
                     LLLAGLFAEGTTTVNEPSTSRDHTERMLAGFGATVEVRSKSVSVTGPQQLTAREITVP
                     GDPSSAAFPVVWAAATPGSSVLIRGVCLN"
BASE COUNT          206 a          340 c          341 g          157 t
ORIGIN      
        1 caggctcatg gcaaagatca gccacctccc ccacctgctg agctacgcgt ccatggggct
       61 cgcggaggac gtgcacatcc acaggcagct cctgggcgca gggttcaggg acttcacccg
      121 catcgccgca tccgaccccc tcatgtggaa ggacatcctc atggacaaca gggacaacgt
      181 cctgcccctc gtggaggcat acatcgagga gctgcatcgg ctgaagacgc tcgtcgaaga
      241 ggaccgcgca acggaactcg aaacggagct ttcccgcttt gcacagatca ggagggcgct
      301 ctatgaaagc ccacggtgag ataacgcccc cgccggacaa gtcgatcacg caccggtgct
      361 tcatgatcgg ctctctcgcg cacgggacca cggtgatacg aaacgccctg gagtccgagg
      421 acatcgcgag cacccgcaag gcgctcgcgg ccctgggcgc gtcgatccgc aaagacggcg
      481 actcctacct cgtggacggg tcaaccctga gggaaccgac acacgtcgta gacgcgggca
      541 actcgggcac cacggcgagg ctcgtctcgg gcatcgtctc ggggatcgag ggcatcaccg
      601 tcatgaccgg cgacgaatcc ctcatcaaga ggcccatggc acgcgtcata aagcccctgg
      661 agctcatggg cgcgaggttc atggcgaggt cgggctcatt cttgcccatg gcggtcaggg
      721 gggggaaact cagggggatc tttcacccca tggaggtggc atccgcccag gtcaagtcgg
      781 cgctgctcct ggcaggcctc ttcgccgagg gcaccacgac ggtgaacgag ccatccacga
      841 gccgggatca caccgagagg atgctcgcgg ggttcggggc gacggtggag gtgagatcga
      901 aatcggtgag cgtcaccggg ccgcagcagc tcaccgcacg ggagataacc gtgccgggag
      961 acccttcgtc cgccgccttc ccggtcgtgt gggccgccgc gacgccggga tcgtcggtgc
     1021 tcataagggg agtctgcctg aatc
//
LOCUS       DAOSCR010000792         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97264_length_1043_cov_2.799595, whole genome shotgun sequence.
ACCESSION   DAOSCR010000792 DAOSCR010000000
VERSION     DAOSCR010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97264_length_1043_cov_2.799595"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..>1043
                     /locus_tag="PLT69_13660"
     CDS             453..>1043
                     /locus_tag="PLT69_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOJ15532.1"
                     /translation="MDHLEIFYRFGVSLVIGLLIGIQREHAYGEEDHAAAANVGLFAG
                     ARTFSLLGLYGCAAAFLGDTAGAPWVTAVLALPAGLLIVVAYALGAWRGWIGMTTQVA
                     GLMTIVVGILCSKGHLVLATALGVATMTLLALKLEIQTFARRVSRQDIYATVKFAVIA
                     ALVLPVLPREGLGPAPFDVIVPYDVWLMVVFISGISF"
BASE COUNT          138 a          343 c          391 g          171 t
ORIGIN      
        1 gtgggaaggg cggggtcttc tccgtgaccc tctttgcgaa ggctcaaggg gaagctctcg
       61 cggccggctg gggagacgaa aacgggcacg gtgtccccgg gcgccgaagc cgtggcctcg
      121 ctcgtcccct ctccgggccg tgcggcatgg agcccgtgac aatgtttcgg cccggggctc
      181 ggggccgcaa caggcaggcg cagccgggcg ggcctgcctg ggcccggccg gccgggtgcc
      241 cgaaacgggt gccgtacgcg cttggggcac cgcctgcggg cggacccggg tggatgggcg
      301 acggggatcg aaaggcgagc gtcgcgcggc ccaaggatgc gcgcggggtt cacgatgcgc
      361 cctgcaggcg gggaaagccc gcctgcgctg acggaatcct tgcccgtcgc cgcagacccc
      421 cgcagtgggc acgctaccct gagaggtagg ccatggatca cctggagatc ttctaccggt
      481 tcggcgtctc tctcgtcata gggctactca taggcatcca gcgcgagcac gcatacggcg
      541 aggaggatca cgccgccgcg gcgaacgtcg gcctcttcgc cggggccagg acgttctcgc
      601 tcctcggcct ctacggttgc gcggccgcct ttctgggcga cacggcgggg gcgccctggg
      661 tcacggccgt gctcgcactg cccgcagggc ttctcatcgt ggtcgcgtat gcgctgggcg
      721 cgtggcgggg ctggatcggc atgaccacgc aggtggcggg tctcatgacg atcgtcgtcg
      781 ggatcctctg cagcaagggt cacctggtgc tcgcgacggc cctcggcgtg gcgaccatga
      841 ccctgctcgc gctcaagctg gagatacaga cgttcgcgag gagggtgtcc cgccaggaca
      901 tctacgcgac ggtcaagttc gcggtgatag cggccctggt cctgccggtg ctgccgcgcg
      961 agggcctggg gcccgcgccc ttcgacgtga tcgtgccgta cgacgtgtgg ctcatggtgg
     1021 tcttcatctc ggggatcagc ttc
//
LOCUS       DAOSCR010000793         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97287_length_1043_cov_2.258097, whole genome shotgun sequence.
ACCESSION   DAOSCR010000793 DAOSCR010000000
VERSION     DAOSCR010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97287_length_1043_cov_2.258097"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1043)
                     /locus_tag="PLT69_13665"
     CDS             complement(<1..>1043)
                     /locus_tag="PLT69_13665"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ15533.1"
                     /translation="ESIEGLSPAIAIEQKAMSNNPRSTVGTVTEIYDYLRVLFARVGR
                     PHCWECGRPIASMTVQQMTQAVASLGQGKRVMILAPVVADRKGEYARLFDRLAKDGFV
                     RVMVDGKPYDLDEPPKLDKNRKHTIEVVVDRIVIREGISPRLHDSLETACRLSDGVVR
                     VEEDGGVSHVYSERHACPVCNTFLRELSPRVFSFNNPHGACSACHGLGVLIEVDPGLV
                     VPDPSRSLSRGAVAPWGVPPGTFAMRTIRRLARLLSFDEDTPFEDLPESTRNVILYGG
                     VIGGPYGERFEGVIPNLARRLAESENGEIGEDTACYLSTRTCPSCRGARLKKEVLHVL
                     VGSKNIAELTDMS"
BASE COUNT          168 a          359 c          331 g          185 t
ORIGIN      
        1 aagctcatgt ccgtcaactc ggcgatgttc ttcgacccca cgagcacgtg gagcacctcc
       61 ttcttgagcc tcgcgcccct gcacgacggg caggtcctcg tgctcaggta gcacgcggtg
      121 tcctcaccga tctcaccgtt ctcgctctcg gcgagtctcc tcgccaggtt ggggatcacc
      181 ccctcgaacc tctccccgta aggccccccg atgacgccgc cgtacaggat gacgtttctc
      241 gtcgactccg ggagatcctc gaagggggtg tcctcgtcga atgacaacag gcgagccaga
      301 cgcctgatgg tcctcatggc gaacgtcccc ggcggcacgc cccatggagc gacggctccc
      361 ctcgagaggg acctcgacgg gtcgggcacc acgagccccg ggtccacctc gatgaggacg
      421 cccaggccgt ggcaggccga acaagcgccg tgggggttgt tgaaggagaa caccctcggc
      481 gagagctctc tcaggaaggt gttgcacacc gggcaggcat gcctctcgga gtagacgtgg
      541 gagacgccgc cgtcctcctc gaccctgacg acgccgtccg agagcctgca ggcggtctcc
      601 agggagtcgt gcaggcgcgg ggatatcccc tccctgatca cgatgcggtc caccacgacc
      661 tcgatggtgt gtttcctgtt cttgtccagc ttcggcggtt cgtccaggtc gtagggcttc
      721 ccgtccacca tgacccgcac gaacccgtcc ttggcgagac gatcgaagag cctcgcgtac
      781 tcgcccttgc ggtcggcgac gacgggggcg aggatcatca ccctcttccc ctggccgagg
      841 gacgccacgg cctgcgtcat ctgctggacg gtcatggacg cgatcggcct cccgcactcc
      901 cagcagtggg gcctgcccac ccgggcgaag agtacgcgga gatagtcgta gatctcggtg
      961 accgtgccga ccgtagacct ggggttgttg gacatggcct tctgctcgat ggcgatggcg
     1021 ggcgagagcc cctcgatgga ttc
//
LOCUS       DAOSCR010000794         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97783_length_1039_cov_3.658537, whole genome shotgun sequence.
ACCESSION   DAOSCR010000794 DAOSCR010000000
VERSION     DAOSCR010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97783_length_1039_cov_3.658537"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PLT69_13670"
     CDS             complement(<1..951)
                     /locus_tag="PLT69_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ15534.1"
                     /translation="MPVRINDIIDEISTYVPDSGLRLVEKAYVFSARVHQGQTRLNGE
                     PYLNHPLEVAYIVAQMRLDVVSVAAALLHDTIEDSLTKREDIKAEFGEEVASVVDGLT
                     KISRIDFRSEEEKQAENFRKMILAMSKDLRVLLIKLIDRLHNMRTLAFHTRQNQLRIA
                     RETLDIYAPLANRLGIHWLQTELENLSFMYLEPEDYRILKAKMDSIAREKEDYLRNFM
                     ARVRLKLEEAGIKSDIKSRIKHIYSVYQKLKRQNISLSNVYDVLGIRIIVDTEAECYA
                     ALGVIHGSWKPIQQRIKDFIAQPKPNGYKSLHTTVIGPGGD"
BASE COUNT          172 a          328 c          288 g          251 t
ORIGIN      
        1 gtcgcccccg ggccctatca cggtggtgtg cagcgacttg tacccgttcg gcttgggctg
       61 ggctatgaag tcttttatcc gttgctgtat cggcttccat gaaccgtgaa tgacacccag
      121 ggccgcgtag cactcggcct ccgtatccac tatgatcctg atcccgagca cgtcgtagac
      181 gttggacagc gatatgttct ggcgcttcaa cttctggtag acgctgtata tgtgtttgat
      241 ccggctcttg atgtccgact ttatccccgc ctcctccagc ttgagcctga cccttgccat
      301 gaagttcctc aggtaatcct ccttctccct ggcaatggag tccatcttcg ccttgaggat
      361 ccggtaatcc tcgggctcga gatacatgaa gctcaggttc tcgagctcgg tctggagcca
      421 gtgtatgccg agcctgttcg cgagcggcgc gtagatgtcc agcgtctccc tcgctatccg
      481 caactggttc tgcctggtgt ggaacgcgag cgtcctcatg ttgtgaaggc ggtctatgag
      541 cttgatgagc agaaccctga ggtccttgct catcgcgagg atcatcttcc tgaaattctc
      601 tgcctgtttc tcttcctccg accgaaagtc gatccggctt atcttggtga ggccgtccac
      661 caccgaggcg acctcctcgc cgaactccgc cttgatgtcc tccctcttgg tgaggctgtc
      721 ctcgatggtg tcgtggagga gcgccgccgc gaccgacacg acgtccaggc gcatctgggc
      781 cacgatgtac gccacctcca gggggtggtt gaggtatggt tcgccgttga gacgcgtctg
      841 cccctggtga accctggcgg aaaagacata ggccttctcg acgagcctga gccccgagtc
      901 aggcacgtag gtggatatct cgtctatgat gtcattgatc cgtacgggca acgctcacac
      961 cctcggattc gagcagcccg acgcagtggg acaccgcctg tcccacgccc accttctcga
     1021 cgcccgaccc cttgcgcat
//
LOCUS       DAOSCR010000795         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_97940_length_1038_cov_2.499491, whole genome shotgun sequence.
ACCESSION   DAOSCR010000795 DAOSCR010000000
VERSION     DAOSCR010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97940_length_1038_cov_2.499491"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLT69_13675"
     CDS             complement(<1..860)
                     /locus_tag="PLT69_13675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ15535.1"
                     /translation="MALHPEDAVHKRGIDVRTLHEVVSIDRGSRLVTVKDLRTGRFAE
                     EEYGVLVISTGARPVVPEIEGMDLPGVFFLKAFQDGIDLKAFIANQKPKNAVIMGGGY
                     IGVEMAESFAKTGMDVTVVEAAGRVMPVMDPEMSALVEDALLKGGVKVITGRKVTALE
                     GDVAVRSVVLDGGGALDAKCVLVSVGVAPNSGIAAKAGLALGPRDAVLVDRYQRTSDP
                     DIFAAGDCCTVYHRALCKDVYIPLGLTANRQGRICGENVAALLKGGMFRPFPGVVGTA
                     VTRVFDLEVAR"
BASE COUNT          165 a          381 c          317 g          175 t
ORIGIN      
        1 cgcgccacct cgaggtcgaa caccctggtg acggccgtac cgacgacacc ggggaagggc
       61 ctgaacatcc cccccttgag gagggccgcg acgttctcgc cgcagatcct gccctggcgg
      121 ttcgctgtca gcccgagggg tatgtacacg tccttgcaca gggcccggtg gtacacggtg
      181 cagcagtctc cagcggcgaa gatgtccggg tcggaggtgc gctggtagcg gtccaccagc
      241 acggcgtccc tgggacccag ggcgagcccg gccttggcgg cgatgccgct gttgggggcg
      301 acgccgacgc tcacgaggac gcacttcgcg tccagggcgc cgccgccgtc gagcacgacc
      361 gagcggaccg ccacgtcgcc ctcgagagcg gtgaccttcc tgcccgtgat caccttcacc
      421 ccgcccttga gcagggcgtc ttccacgagc gcgctcatct cggggtccat gaccggcatg
      481 accctcccgg cagcctcgac caccgtcaca tccatgcccg tcttggcgaa cgactccgcc
      541 atctccaccc cgatgtagcc gccgcccatg atcacggcgt tcttcggctt ctggttcgcg
      601 atgaacgcct tcaggtcgat gccgtcctgg aatgccttga ggaagaacac gcccggcagg
      661 tccatgccct cgatctcggg cacgaccggc ctcgctcccg tggagatcac gaggaccccg
      721 tactcctcct cggcgaacct ccccgtcctc aggtccttca ccgtgactag gcgcgagccc
      781 ctgtctatgg agacgacctc atgaagggtc cgcacgtcta tgccgcgctt gtggacggcg
      841 tcctcgggat gcagcgccac cagttcgtca tgttcgcgta cctgaccaga tacgtagtac
      901 ggtatccctc aggcgccgta ggagatgtag gcctccctgc cgaagacgac gacctccgcg
      961 tcgggcgctt cgcgcttgat gacgctcgca gcgctcatgc ccgaggccac ggccccgatg
     1021 acgacaacac gtctcatg
//
LOCUS       DAOSCR010000796         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_98354_length_1035_cov_2.548980, whole genome shotgun sequence.
ACCESSION   DAOSCR010000796 DAOSCR010000000
VERSION     DAOSCR010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98354_length_1035_cov_2.548980"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..980)
                     /locus_tag="PLT69_13680"
     CDS             complement(411..980)
                     /locus_tag="PLT69_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15536.1"
                     /translation="MFKTLKSDLQAVLKRDPAAKSIWEIMLCYPGFRAVRMHRRANRL
                     YRHKWHLLARMTSEWCRFTTGIDIHPGASIGERFFIDHGTGTVIGETCIIGSNVRLYQ
                     GVTLGAKSFPKDEAGRLVKGIPRHPIVEDDVIIYSGTTILGRVTIGRGSVIGGNVWLD
                     HDVAPYSTVIQADGASTVVVPSGKGASIP"
BASE COUNT          178 a          321 c          337 g          199 t
ORIGIN      
        1 cgaggtggac acgggtgcgc tcgtgatcgt gatcgccctc atacccgcct ggtggatcgt
       61 gggtgtcctc gtcaagacat ggtcagtgaa gtgatgcgca ccgaacgccc ttgggcactt
      121 ccttgaaggg ggggtcttcc ccggggcccg cgggcctcag ggagaagctc ccctcgagag
      181 aagcgaagat ccggatcttc accgaggctc gggatcctgg tggaggggtt ctcctcaaga
      241 gaggcggggt ccccggcctt ccccagggcc cgcgggcctg agtgctcatc accggacgcc
      301 gacgagctct gtcctcggcg gacgacgacg ccgtcttttg cacgccccct tgcggcgctc
      361 gaggggaaca tccccgcagg tcaagagcgg gcggccgtct tgccgggtgc ctaggggatc
      421 gaagccccct tgccggacgg gaccaccacc gtggatgcgc cgtccgcctg gatgaccgtg
      481 ctgtagggcg ccacgtcgtg atccagccac acgttgccgc cgatcaccga gccccttcct
      541 atcgtgaccc tcccgaggat ggtggtcccc gagtagatga tcacgtcatc ctccacgatg
      601 ggatgacggg ggatgccctt cacgagcctg ccggcctcgt ccttggggaa gctcttcgcg
      661 ccgagcgtaa cgccctggta gagcctgacg ttggagccga tgatgcaggt ctcgcctatg
      721 accgtgccgg tcccgtggtc gatgaagaac cgttccccga tggaagcgcc cgggtggatg
      781 tcgatccccg tggtaaagcg gcaccattca gaggtcatcc gcgcgagcaa atgccatttg
      841 tgacggtata agcggttggc tctgcggtgc atgcgcacgg cgcgaaagcc aggatagcag
      901 agcatgatct cccagatgga ttttgcagcg gggtctcgtt tgagcaccgc ttgcagatcg
      961 cttttcagcg ttttgaacat atggcttcgt gttcctttcg cggcggaatg cccgcctgaa
     1021 acaataaaaa taagc
//
LOCUS       DAOSCR010000797         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_98939_length_1031_cov_2.738730, whole genome shotgun sequence.
ACCESSION   DAOSCR010000797 DAOSCR010000000
VERSION     DAOSCR010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98939_length_1031_cov_2.738730"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          186 a          316 c          338 g          191 t
ORIGIN      
        1 ccaccacctt cgattccctc ctttcgcatc gagccggatg cggagcccgc cgcgcaaggt
       61 tctcctgcac caagcccacg tccaggtgct cacgccgata tgtgaggcag tcgccgccgc
      121 atgaccttcc caagcctggt gtgcgcattc ggacaggcgt gccctttctc gagaggccgt
      181 ctttgtctgg tccggcctgg ggtcgcctgt gtgcggcacg ctggggcgcg aggccgcaag
      241 ccatgggcgc ccacgagggt cctcagcccg accgacagac gtaaaccgcc cccatcgcga
      301 gccgatgtcg cccctgtccg cggtcgtacc cgattcccgt gagcatgagt cgagccgggt
      361 tcatgagagc ggtctcgacg gttccattgg tcatctccga gggctgggag gtcgacagat
      421 gccgtctgca tgaaccgcgg gcggaccggg cctgagcgag cgtaaggaac ggcaccttga
      481 gagggcggtc actatccgtt cgaagggaag gagtgccccg gccatgtccg gtggcttccc
      541 ggcggcttgt ccgcccgtca ggtgcgcgag ccaggggcgt ggcgcgaggg ttcgtaaggg
      601 cgcatcaagc cgcaggggaa gcggaagccc tcgtgcggtg ttccgtgagc cgtgacaagg
      661 cgaaacgccg tgtaacgctc ataaaaagca agaaaccatg agaaatatcg ccctgtggat
      721 tctcttgagg tccaggcggg tcgaatccct ctatggcctc tatttttccg tccttgtgtt
      781 tttatactga aggagtatga aggctcggaa agaaaaaggc ctccggtata ggggggcgac
      841 ccagtgagcc tgcagagcgg gaggacacgg accagcgatg gaaagagcgg cgtcttcgag
      901 tacttcgttc ttctttaaga tcctcgtctt catggcagtg cttgccctgt tctcggggtg
      961 tgcaaagaag accccggagc cgggcgtcga gacgccccgc acgaccgagg ccgctcccgc
     1021 cccaggacat g
//
LOCUS       DAOSCR010000798         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_99634_length_1026_cov_3.244078, whole genome shotgun sequence.
ACCESSION   DAOSCR010000798 DAOSCR010000000
VERSION     DAOSCR010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99634_length_1026_cov_3.244078"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLT69_13685"
     CDS             complement(<1..339)
                     /locus_tag="PLT69_13685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOJ15537.1"
                     /translation="MKDDIVARRYAKALYDLGREEGLTDVFLSDLETVLSVMAASEEF
                     RSVMESPLYDIELKRRILSQVASEVKVHAYTKSFLDILLEKDRFVYADSILEAYRQII
                     DESSGRVRAVV"
     gene            complement(342..938)
                     /locus_tag="PLT69_13690"
     CDS             complement(342..938)
                     /locus_tag="PLT69_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOJ15538.1"
                     /translation="MRRRLADTAVRVAPALALFVLAAAGIALGSEGGGGHGEGGTTKW
                     LDLLWRTVNFIILAALLWKLLADKVKKYFIERREEIAQLIEEADRAKADAQAQFAEMK
                     KKLANVERDIEEIKTAIMGELDSEKARIIEEGRLAAERIIQQAKWTADQEVVKARKEL
                     KEQVVDMAADLASGIVTKSMTPEDQKRILEEYLDRVAR"
BASE COUNT          160 a          369 c          275 g          222 t
ORIGIN      
        1 cacgacggcc ctcaccctgc ccgaagactc gtcgatgatc tgccggtacg cctcgagtat
       61 ggagtcggcg tacacgaacc ggtccttctc gagcagtatg tcgagaaagc tcttcgtgta
      121 agcgtgcacc ttcacctcgg atgcgacctg ggacaggatc ctccgcttga gctcgatgtc
      181 gtagaggggg ctctccatca ccgacctgaa ctcctcgctg gcggccatca ccgacaggac
      241 ggtctccagg tcggagagaa acacgtcggt cagcccctct tccctcccca ggtcgtagag
      301 ggccttcgca tatcgccgtg caacgatgtc gtctttcaag gtcacctcgc taccctatcg
      361 agatattcct cgagtatccg cttctgatcc tcgggggtca tgctcttcgt cacgatgccg
      421 gacgcaaggt ccgcggccat gtccaccacc tgttccttga gctccttgcg ggccttgacg
      481 acctcctggt cagccgtcca cttggcctgc tggatgatcc gctcggccgc gagcctgcct
      541 tcctctatga tccgggcctt ctcgctgtcc agctctccca tgatggccgt cttgatttcc
      601 tcgatgtccc tctccacgtt cgcgagcttc ttcttcatct cggcgaactg cgcctgggca
      661 tcggccttcg ccctgtccgc ctcctcgatc agctgggcga tctcttccct gcgctctatg
      721 aaatacttct tcaccttgtc ggccaggagc ttccacaaca gcgccgcgag gatgatgaag
      781 ttgaccgtgc gccagaggag gtccagccac ttggtcgtgc cgccctcacc gtggccgccg
      841 cccccctcgg acccgagggc gatgccggcc gccgcgagaa cgaagagcgc aagggccggg
      901 gccacccgaa cagccgtgtc agcaagacgc ctcctcatgc aaccctcctc cccaggatct
      961 gttcggctat cgtcgcggcc atgaccccga gctcgggctc gagctccttc ttggcctgcg
     1021 ctatct
//
LOCUS       DAOSCR010000799         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_99933_length_1024_cov_3.817337, whole genome shotgun sequence.
ACCESSION   DAOSCR010000799 DAOSCR010000000
VERSION     DAOSCR010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99933_length_1024_cov_3.817337"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /gene="rpsL"
                     /locus_tag="PLT69_13695"
     CDS             complement(<1..249)
                     /gene="rpsL"
                     /locus_tag="PLT69_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ15539.1"
                     /translation="MPTLNQLVRKGRKAVGRRQKTRALQACPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNGYEVTSYIPGEGHNLQEHSVVMIR"
     gene            complement(407..1024)
                     /locus_tag="PLT69_13700"
                     /pseudo
     CDS             complement(407..1024)
                     /locus_tag="PLT69_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          150 a          339 c          289 g          246 t
ORIGIN      
        1 cctgatcatc accaccgagt gctcctggag gttgtgtccc tcgcccggga tgtacgacgt
       61 cacctcgtag ccattggtga gcctgacgcg ggcgaccttc ctgagggccg agttcggctt
      121 cttcggcgtg gtggtgtaca ccctcacgca tacgcccctc ttctgcgggc acgcctggag
      181 agccctcgtc ttctgccgcc tcccgacggc cttcctgcct ttcctgacga gctggttcag
      241 tgtgggcatc gatccttctt ccttctcacg taatcgctga gaaaatccct gaccgcttga
      301 tcagacagac gcagtcttct atcaacgcga acttcgatct gtcaaggact ttctaagcca
      361 gttcctcttc gtaaaggtcg taatcgctga cctctatatc caccttcctg taacgcggga
      421 aacccgtccc ggcggggatg agcctgccca tgatgacgtt ctccttgagc cccaccaggt
      481 ggtccacctt gccctccacg ctcgcctcgg tgagcacgcg ggtggtctcc tggaaggagg
      541 ccgccgagat gaagctctcg gtgttgaggc tcgccttcgt gatgccgagc aggtacggct
      601 cggcgagcgc aggcctgagc ccctggctga tcatgcgctg gttctcctcc tcgaagacga
      661 acttgtccac gaattcgccc tcgatcatct ccgtgtcgcc ggggtccttg atcttgaccc
      721 tcttgagcat ctggcggacg atgacctcga tgtgcttgtc gttgatgcgc acgccctgca
      781 acctgtagac ctgctggatc tcgttgacga ggtacttggc gagctccttc tcgcccctga
      841 tcctgaggat gtcgtgcggg tcgaccgcgc cgtccatgag cggctcgccg gcccggacca
      901 ggtcgccctc gagcacggtc acgtgcttgc tcttgggtat catgtactcc ttgggctggc
      961 ctatctcggg cgtgatggtg accttgcgct tgcccttggt gtccttgccg aaggtcacct
     1021 cgcc
//
LOCUS       DAOSCR010000800         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_100116_length_1023_cov_2.506198, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000800 DAOSCR010000000
VERSION     DAOSCR010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100116_length_1023_cov_2.506198"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLT69_13705"
     CDS             complement(<1..931)
                     /locus_tag="PLT69_13705"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOJ15540.1"
                     /translation="MERDLNHLLSLGYEKDEVLAAALHAVRENYLTKVAVQSMIGEEV
                     CFQGATAKNAALVAAFEQRLERPIFVSKYPHLTGALGAALILDEEHSGATAFRGTGIH
                     KRTIELCTEVCTICANHCKLTVAEIDGQTVASGFLCGREYDSKAGPRSNPSGFDLMRE
                     RKRAFSRKRPKTYTNSVVIGLPDALHMSEDIEFWRFFFDRLGVRTVTRPASEARHRGR
                     APSVGAEFCMPVRHLFDSVWKLLPEVDHVFLPVYLEDEDEAQRKGARRQYCYVTQYAP
                     SLVALRLAGEAGPGRILTPVVEPGQVHVRTTMSL"
BASE COUNT          168 a          353 c          316 g          186 t
ORIGIN      
        1 acaggctcat cgtcgtccgt acgtgcacct gacccggctc cacgaccggg gtgagaatcc
       61 tgcccggtcc cgcctcgccc gcgagacgca aggctacgag ggagggggcg tactgcgtga
      121 cgtagcagta ctgcctgcgc gcccctttcc tttgcgcctc gtcctcgtcc tccaggtaga
      181 cgggcagaaa gacgtgatcc acctcgggaa ggagcttcca cacgctgtcg aacaggtgcc
      241 tgaccggcat gcagaattcc gcccccaccg agggcgcccg ccctctgtgc cgcgcttccg
      301 acgcaggccg ggtgacggtc cttacgccca gccggtcgaa gaagaagcgc cagaactcga
      361 tgtcttcgct catgtgcagt gcatccggga gcccgatgac cacggagttt gtgtacgttt
      421 tcggtctctt gcgtgaaaag gccctcttgc gctccctcat gaggtcgaac ccggaagggt
      481 tcgaccgggg gcctgccttc gagtcatact ccctgccgca caagaagcca ctcgccaccg
      541 tctgcccgtc gatctcggcc accgtgagct tgcaatggtt cgcgcagatg gtgcacacct
      601 cggtgcacag ctcgatcgtc ctcttgtgga tcccggtccc cctgaacgcg gtggcccccg
      661 agtgttcttc atcgaggatg agggccgcgc cgagggcacc ggtcaagtgc gggtacttcg
      721 acacgaagat gggcctttcc aggcgctgct cgaacgcagc gaccagcgcc gcgttcttcg
      781 cggtggcgcc ctggaagcac acctcctcgc cgatcatcga ctgcaccgcc accttggtga
      841 ggtaattctc gcgcaccgca tgcagggcgg cggccaggac ctcgtctttc tcgtacccga
      901 ggctcaggag gtggttgagg tcgcgttcca tgaacacggt gcaacggtcg ctcgacgcag
      961 gggcccccct cccgagggcc cgttcggaaa cttcggcaag cgggcacccg agcctcgagg
     1021 ctt
//
LOCUS       DAOSCR010000801         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_100256_length_1022_cov_2.539814, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000801 DAOSCR010000000
VERSION     DAOSCR010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100256_length_1022_cov_2.539814"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLT69_13710"
     CDS             <1..697
                     /locus_tag="PLT69_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ15541.1"
                     /translation="GLLKIRSIYGRISRALVGACPHTPPAALVEALENTQEALRTALG
                     RDACAEAMAMLVMDHPQLVEHSLRVALIGSALVGRMAGTRVSRSASVAIAEGFIVHDI
                     GMERVPQAITLKASPLDTFERSLVQMHPSWGCRSIKGFGLEDDEVRSIVACHHERPGG
                     RGYPSGKAWDDLPLPVRVCAVADVFEALTSPRPYRDKLATFEALRQVYREFTQNGPDE
                     LFGALVELCSSFG"
BASE COUNT          154 a          331 c          356 g          181 t
ORIGIN      
        1 gggcctcctc aagatcagga gtatctacgg caggatatcc cgcgcactgg tcggtgcatg
       61 cccgcatacg cccccggcag ccctcgtgga ggcgctcgag aacacgcagg aggccttgag
      121 gaccgctctc ggccgggacg cctgtgccga ggccatggcc atgctcgtca tggaccaccc
      181 gcagctggtg gagcactccc tgcgcgtggc cctcatcggg tcggccctcg tgggaaggat
      241 ggccggcacg agggtctcga ggagtgcgtc ggtggcgatc gcggaagggt tcatcgttca
      301 cgacataggc atggagcgcg tgccgcaggc gatcacgctc aaggcctcgc cgctggacac
      361 cttcgagaga tcgctcgtcc agatgcaccc gtcctggggg tgcaggagca tcaagggctt
      421 cggactcgag gatgacgagg tgaggtccat tgtcgcgtgt caccacgaga ggccgggtgg
      481 gcgagggtat ccctccggga aggcatggga cgatctcccc ctgcctgtga gggtgtgcgc
      541 ggtggcggac gtcttcgagg cgttgacctc gcccaggccg tatagggaca agctcgcgac
      601 gttcgaggcg ctcaggcagg tctaccggga gttcacgcag aacggccccg acgagctgtt
      661 cggcgcgctc gtcgagctgt gttcgtcctt cgggtgagaa ccccggactc ccacacccgg
      721 catcgcgcac cttctcgagg ggttcggcct taagacccag caggggcgac cgacaatcga
      781 ccccagggtc tgtccatgcc gagacgtggt acgcatcctc ggcgggcacc gcggtgtcca
      841 gtcggtgcat ggtcgatcga tcccttcccc ggaccgtctc ttgcacgggc tccgtacccg
      901 gccgacggcg caggcggggt gctggtcctt gacttgtccc gggaatcgtt gtttattcgc
      961 gctatgttcc tcatcgtctc cggcggtcag ccgccgggcc gggcgctgat cgcgctggcc
     1021 gc
//
LOCUS       DAOSCR010000802         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_100863_length_1018_cov_2.713396, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000802 DAOSCR010000000
VERSION     DAOSCR010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100863_length_1018_cov_2.713396"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLT69_13715"
     CDS             complement(<1..231)
                     /locus_tag="PLT69_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ15542.1"
                     /translation="MKGRVKIFTYGCQMNDLDSLKMYTELSKAGYVPTDSTREADVIV
                     VNTCSVRQKACEKAMSNLGRLASVKLRKPSAVI"
     gene            535..975
                     /locus_tag="PLT69_13720"
     CDS             535..975
                     /locus_tag="PLT69_13720"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HOJ15543.1"
                     /translation="MCANKRGVTLMEVIITVVIVGIIAAIAIPAYTGYVNRTRRAEAV
                     TALETVALYEEKAFAEANTYESLPDLIANRRLPDPNANSRYYQIDVDPINGDYAEGFI
                     ATATPINEQAGDGIVFGIDSNGNRGTVSGGVVVQNQDLWNSLRR"
BASE COUNT          203 a          296 c          331 g          188 t
ORIGIN      
        1 gatgaccgcg gacggtttcc tgagcttgac cgacgcgagc ctgcccaggt tgctcatggc
       61 cttctcgcag gccttctggc ggaccgagca cgtgttcacc acgatgacat cggcctctct
      121 cgtggaatcc gtcgggacgt accccgcctt ggagagctcg gtgtacatct tgaggctgtc
      181 caggtcgttc atctggcagc cgtaggtgaa gatcttcact ctgcctttca tgagggcatc
      241 catatatgct gctgttctgc gcgtcaagga ctgcagcggg gagcctgggg cttccgaacc
      301 ccgtgcggga gcctgggctt cgaaggccga ggatgcccgc ccccggttgt ctttcggcac
      361 ggccgggaag agcgcgtgca acgtgccggg ggttttcgcc atacaggccg tctccggtag
      421 ggtagacatg gtgtgggccg acacacggtt tgaggccgca cactttcatc tcttgaaaag
      481 gcccgatccg gccgatacag tatatgagtc ctacccggag aaggaggtcc tgacatgtgt
      541 gcgaacaaaa ggggtgtgac cctcatggag gtcatcatca cggtggtcat cgtggggatc
      601 atcgccgcca tagcgatccc agcctacacg gggtacgtca acaggacgag gagggcggag
      661 gcggtgacag ccctcgaaac cgtcgccctc tacgaggaga aggcctttgc cgaggcgaac
      721 acctacgaga gcctgcctga tctcatcgcg aacaggaggc ttccggaccc caacgcgaac
      781 agcaggtatt accagataga cgtggatccc atcaacgggg actatgccga gggattcatc
      841 gcgactgcga ccccgatcaa cgagcaggcg ggtgacggca ttgtcttcgg gatcgactcg
      901 aacggcaaca ggggcaccgt gtccggcggg gtggtcgtcc agaaccagga cctctggaac
      961 agcctgagga gatgagcctt ggggatccga ggacccgagg ctggaagact tcagccga
//
LOCUS       DAOSCR010000803         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_101303_length_1015_cov_4.454167, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000803 DAOSCR010000000
VERSION     DAOSCR010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101303_length_1015_cov_4.454167"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLT69_13725"
     CDS             complement(<1..388)
                     /locus_tag="PLT69_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ15544.1"
                     /translation="MMDRFPTSRIVPVAIAAAVVIVLLSMGQYRSLSSGLSPVREGYY
                     IAERVITAPFRFAASAWADYVALLDTRRQNKELIRELEVLKFKCQAVRGLELENEKLR
                     AMLDFKTSHPELKLSPARIVTHDITAM"
     gene            complement(385..>1015)
                     /gene="mreB"
                     /locus_tag="PLT69_13730"
     CDS             complement(385..>1015)
                     /gene="mreB"
                     /locus_tag="PLT69_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HOJ15545.1"
                     /translation="VYIVAEPMAAAIGAGLPVTEPISSMIVDIGGGTTEVAVISLSGI
                     VYTNSVRVAGDRIDQAIMQYIKREYNLLIGERTAEIIKIMIGSAAADDVEKTIEVKGR
                     DLVDGIPKIFTISSEEIRKACQGPVRTIIDAVRVALEQTPPELAADIVDRGIVLTGGG
                     ALFKNLDKLLREETGLPVIIAEDPLSTVARGAGKLLDDIELLRDVTIPS"
BASE COUNT          165 a          326 c          316 g          208 t
ORIGIN      
        1 acatggcagt tatgtcgtgt gtcacgatgc gcgccgggga gagtttcaac tccgggtgcg
       61 aggtcttgaa gtcgagcatg gcgcgaagct tctcgttctc cagctcgagg cccctcacgg
      121 cttggcactt gaacttcagg acctccagct cacggatgag ttccttgttc tgtcggcgtg
      181 tgtccaggag ggccacgtag tcggcccacg ccgacgccgc gaacctgaac ggggcggtga
      241 tgacccgctc ggctatgtag tacccctccc tgaccgggct cagcccactc gagagcgagc
      301 ggtattggcc catggacagc agcacgatga cgaccgctgc ggctatggct accgggacga
      361 tcctcgatgt ggggaaacgg tccatcaact cggaatggtc acgtccctca acagctctat
      421 gtcgtcaagg agcttgcccg cgcccctggc gaccgtcgac aacgggtcct ccgctatgat
      481 gacaggcaga ccggtttctt cacgcaggag cttgtcgagg ttcttgaaca gcgcgccgcc
      541 cccggtgagc acgatgcccc tgtcgacgat gtccgccgcc agctcaggcg gcgtctgctc
      601 gagggcgacc cgtaccgcgt cgatgatggt cctcaccggt ccctggcagg ccttgcggat
      661 ctcctcggag cttatggtga agatcttcgg gatgccgtcc acgaggtccc tccccttgac
      721 ctcgatggtc ttctccacgt cgtccgccgc ggcggaaccg atcatgatct tgatgatctc
      781 tgcggtacgc tcgcctatga ggaggttgta ctccctcttg atgtactgca tgatggcctg
      841 gtcgatcctg tcgccggcga cgcgtacgct gttcgtgtac acgatgcccg acagcgagat
      901 gaccgcgacc tcggtggttc ccccgccgat gtccacgatc atggagctga tgggctcggt
      961 caccgggagt cctgcgccga tggcggcggc catgggctcg gccacgatgt agacc
//
LOCUS       DAOSCR010000804         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_101604_length_1013_cov_3.078288, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000804 DAOSCR010000000
VERSION     DAOSCR010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101604_length_1013_cov_3.078288"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..>1013)
                     /locus_tag="PLT69_13735"
     CDS             complement(103..>1013)
                     /locus_tag="PLT69_13735"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOJ15546.1"
                     /translation="EHMSGVPYISQDARWGCRLQDKVFVDALIRALHCGSHVQPLDDT
                     SPIKGLPLFDVYKGKPYIMGQTAEFVAQMHGITRQEMDEVALRSHNNAERATKEGDFK
                     EEIVPVELPQKKGKPPVIFDKDEHFRPNLTMDDLASLPPAFIPKTGKVTAGNSSGLND
                     GASALVLMGADKAKELGIAPLARIRAFGRGAVHPSIMGLGPVPAVKDLLRRNPDLNLE
                     SFELIELNEAFAAQYLGCEKELGLNREITNVNGSGIGLGHPVGSTGSRIIVTLIHALK
                     KRGKTLGLATLCGGGGVSMATAVEML"
BASE COUNT          145 a          328 c          336 g          204 t
ORIGIN      
        1 atcaaaagtg cgaaacattc ttgacaccac cccgggctgc ccccacactc gagaggcact
       61 gggatgtgag gccgctcttg tggtgtcggc cgcgatcagc cgtcagagca tctccacggc
      121 cgtggccatg gagaccccgc ctccgccgca cagggtcgcg aggccgaggg tcttgccccg
      181 cttcttgagc gcgtggatga gcgtgacgat gatgcgggag ccggtgctgc ccaccgggtg
      241 gccgagccct atgccgctgc cgttgacgtt ggtgatctcg cggttgagcc cgagctcctt
      301 ttcgcacccc aggtattggg ctgcaaaggc ctcgttgagc tcgatgagct cgaagctctc
      361 caggttcagg tcggggttcc tcctgagcag gtccttgacc gccgggacag ggcccaggcc
      421 catgatcgag gggtggaccg cccctctccc gaaggcgcgg atgcgtgcca ggggggcgat
      481 gccgagttcc ttggccttgt ccgcccccat cagcacgagg gccgaggcgc cgtcgttgag
      541 ccccgaggag ttccctgcgg tcaccttgcc ggtcttgggt atgaaggccg gcggcaggct
      601 ggcgaggtcg tccatggtga ggttcggcct gaagtgctcg tccttgtcga agatcacggg
      661 tggcttgccc ttcttctggg gaagctccac gggcacgatc tcctccttga agtccccctc
      721 tttcgtcgcg cgctcggcgt tgttgtggct cctgagcgcc acctcgtcca tctcctgcct
      781 cgtgatcccg tgcatctggg ccacgaactc cgcggtctgg cccatgatgt agggtttgcc
      841 cttgtacacg tcgaacaggg gcagcccctt tatcgggctc gtgtcatcga gcggctgcac
      901 gtgtgagccg cagtggagcg cccgtatgag cgcgtcgacg aagaccttgt cctggagacg
      961 gcagccccac ctggcgtcct gggagatgta cggtacgccg gacatgtgct cca
//
LOCUS       DAOSCR010000805         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_102074_length_1010_cov_3.654450, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000805 DAOSCR010000000
VERSION     DAOSCR010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102074_length_1010_cov_3.654450"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLT69_13740"
     CDS             <1..247
                     /locus_tag="PLT69_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ15547.1"
                     /translation="TSMDGDGTLAGYDIPFTRAIADAVSIPVVASGGAGTLEDFSRAV
                     LEGHARILLAASVFHFRTFSIAQVKEHLRAKGIPVCS"
     gene            complement(424..>1010)
                     /locus_tag="PLT69_13745"
     CDS             complement(424..>1010)
                     /locus_tag="PLT69_13745"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOJ15548.1"
                     /translation="SMIESVRPGEITSEILNLSELEIRECTHCNFCLTGQEEGRYCSI
                     LDDAQAVFEKVERADIVILASPVYFMRTSAMMAAFIDRLRVFVFGNLARSRLRNKVGV
                     SAAVSWMRNGGLETTHLTHLYAFMTLEMIPVGLHGGTCPLGASGVSGTRGALGREGGR
                     AQGVELDEAGLESAARVMARAIELARMVRKDGSP"
BASE COUNT          162 a          372 c          303 g          173 t
ORIGIN      
        1 cacgagcatg gacggtgacg ggacgctcgc gggctacgac ataccgttta cgcgcgccat
       61 agccgacgcg gtgagcatac cggtggtcgc atccggcggg gcggggacgc tggaagactt
      121 cagccgcgcc gtgctcgagg gccacgcccg catcctcctt gcagcctcgg tgttccactt
      181 caggaccttc agcatcgccc aggtcaagga acacctcagg gcgaaaggta tcccggtctg
      241 ttcctgaggc cccctcggcg cctgttcgac ggcatggcgt atccgtgcag cgaggccgcg
      301 cctgtcggcg tgaggtcttc gcatgccccc ggcatcgtcg agccatggca cacgaggctc
      361 acgccttctc ccgccgcccc ctagcctcag acgccctctt cgccccgtgc cagacgagcc
      421 gcatcacggg ctcccgtcct tcctcaccat ccgcgcaagc tcgatcgccc gtgccatgac
      481 gcgggccgcc gactcgagcc cggcctcgtc cagctcgaca ccctgggccc gccccccttc
      541 ccggcccaaa gcccccctgg tgcccgagac cccggacgcc cccagggggc atgtccctcc
      601 atggaggccg accgggatca tctcgagggt catgaaggcg tagaggtgcg tgaggtgggt
      661 ggtctcgagc ccgccgtttc tcatccagga caccgccgcg ctcaccccca ccttgttcct
      721 cagacgagag cgcgcgaggt tgccgaagac aaagaccctg agcctgtcta tgaaggcggc
      781 catcatcgcg ctcgtgcgca tgaagtacac ggggctggca aggatcacga tgtccgcgcg
      841 ttccaccttc tcgaacaccg cctgggcgtc gtccaggatg gagcagtacc tgccctcttc
      901 ctggccggtc aagcagaagt tgcagtgggt gcattccctg atctcgagct cggaaaggtt
      961 caggatctcc gaggttatct ctcccggccg cacgctctcg atcatcgact
//
LOCUS       DAOSCR010000806         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Deltaproteobacteria bacterium isolate AMR_MDS_1852
            NODE_103267_length_1002_cov_3.630412, whole genome shotgun
            sequence.
ACCESSION   DAOSCR010000806 DAOSCR010000000
VERSION     DAOSCR010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863381
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Deltaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Deltaproteobacteria bacterium
            Bacteria; Myxococcota; Myxococcia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:57:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,751
            CDSs (total)                      :: 2,721
            Genes (coding)                    :: 2,689
            CDSs (with protein)               :: 2,689
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 9 of 32
            Pseudo Genes (incomplete)         :: 18 of 32
            Pseudo Genes (internal stop)      :: 6 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Deltaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103267_length_1002_cov_3.630412"
                     /isolate="AMR_MDS_1852"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026735"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLT69_13750"
     CDS             complement(<1..388)
                     /locus_tag="PLT69_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOJ15549.1"
                     /translation="MNTVVCLKQVPDTETLIKVAGTSIATEGIKWVINPYDEFAVEEA
                     IRQKEKMNAGKVTIVSLGPDRAIESIRTALAMGADDAVHINDGAYYDTADPYATAQVL
                     AAAIKGLTYDAVFLGKQAIDDDAGQVP"
     gene            complement(446..>1002)
                     /locus_tag="PLT69_13755"
     CDS             complement(446..>1002)
                     /locus_tag="PLT69_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ15550.1"
                     /translation="GFYNSKVSEIAREAHVADGTIYLYFKNKDDILISLFEEELTRII
                     RTLKTELESIDDPRDKLIKFCDNHLTIVESDRALAEVMQVELRQSNKFMREYKNKHFI
                     AYLNIIADIVAQGQEEGIFDDGLRPELCARVIFGSLDELSTFLITAKRRRYDVHEVAV
                     MVGKMFLNGMLKAKQPAEKDTGKG"
BASE COUNT          161 a          312 c          280 g          249 t
ORIGIN      
        1 ccggcacctg tccggcgtcg tcgtcgatgg cctgcttgcc gaggaacacc gcgtcatagg
       61 tgagcccctt gatggcggcg gccagcacct gggcggtggc gtacgggtcg gccgtgtcgt
      121 agtacgcgcc gtcgttgatg tgcaccgcgt cgtccgcgcc cattgccaag gcggtgcgga
      181 tcgactcgat ggccctgtca gggcccagcg agacgatggt caccttgccc gcgttcatct
      241 tctccttctg acggatcgcc tcttcgacgg cgaactcgtc gtacggattg atgacccact
      301 tgataccctc tgttgcgatg gatgtacccg cgaccttgat caaggtctcc gtgtcaggta
      361 cctgcttcaa gcacacaacg gtattcacac caaacctcct tcctgggttt tttatccctc
      421 gggcttctct gtgtcgagcc ctctcttatc ccttgcccgt gtccttttca gcgggctgct
      481 tcgccttgag catgccattc aagaacatct tgccgaccat gaccgccacc tcgtgaacgt
      541 cgtacctcct gcgctttgcg gtgatcagga aggtggagag ctcgtccaag gacccgaaga
      601 tgaccctcgc gcagagctcg ggcctcaatc catcgtcgaa gatcccctct tcctgcccct
      661 gagccacgat gtccgcgatg atgttgaggt aggcgatgaa gtgcttgttc ttgtattccc
      721 gcatgaactt gttcgactgc ctgagctcca cctgcatgac ctccgcgagc gccctgtcgg
      781 actccacgat ggtgaggtgg ttgtcgcaga acttgatcag cttgtcgcgc gggtcgtcta
      841 tggattcgag ttccgtcttg agggtcctga tgatcctcgt gagctcctcc tcgaagagcg
      901 atatgaggat gtcgtccttg ttcttgaagt agagatagat cgtcccgtcc gccacgtggg
      961 cttctctcgc gatctccgag accttggagt tgtagaatcc ct
//
